LOCUS       DAOPXN010000001        59413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_470_length_59413_cov_5.202180, whole genome shotgun sequence.
ACCESSION   DAOPXN010000001 DAOPXN010000000
VERSION     DAOPXN010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 59413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59413
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_470_length_59413_cov_5.202180"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..3388
                     /gene="recJ"
                     /locus_tag="PKM49_00005"
     CDS             245..3388
                     /gene="recJ"
                     /locus_tag="PKM49_00005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNY81227.1"
                     /translation="MKCEWVKLCPDQAIIDRISQRHGISGILARILANNGIVEDVEIE
                     EYLYPERTGFYDPFLMQDVDKVVDYLIKIRQKKEPIVIYGDYDVDGVTSTALVYSILH
                     QLGWKVNYYIPSRLEEGYGLSQAAIIDLFNKKNRNIITVDCGISSFKEVDYANFLNFK
                     VIITDHHEVQEKLPNALAVVNPKRRDDRYPDKGLAGVGVAFKVMDALLRRLGNPIDIM
                     EYLDLVTLGTVADIVPLKGENRVIVRKGMRLIAETKRIGLKKLMEISGINVEQIMTQD
                     IGFKIAPRLNAVGRLESAYAALKLLLSQDEAEGMEIARYLSSQNEKRQFIENQIYQDA
                     ETMLKNDRDLQEAPVIVLWKEDWHPGVIGIVSSRLSAKFYKPTLLLSLDYKDGVARGS
                     GRSIEQVNIMDLLSRANVKGLEYGGHPMAAGFTLPAQELTVFREQIFEAYRALYKEEV
                     FTSKIYLDAELPIDEINESLLDSLEILKPFGQCNHEPAFLIKNASVDRLKILGNHSQH
                     IRLILKQGSRFLDCIGFNLSERLEEFRYIRPNLLKADCVGNLKAFWHFGSKQIQFSIK
                     DIHFYLDPAVKEVERTTKALFSISKKNEPTSAEPGSENLETLHADMRQRLFSKYPLLQ
                     KAFSDQSRYAIFASPKTQDAVLSLKCLNAISKGQRLLLVTPSASFSVFRSQVFKQFDI
                     FPTVSNVVLELSSSSSACTIVTIPYLIAHYEELKRDFHEFVFLDIEYLFHEKMEARPV
                     LRQLADLLRTKTPPFTYVGSFLLPEKKAALTTFFPLSGLLTEPMKSTRIRLVDRRGIA
                     DPLSYIQQQVKNGEFVCVSVQKPERTVGLTKEFGKMLSEHFHNGEVVFYNQQLKPFQK
                     SKIEDLVMENKARLLITTPDFSSHLKFPANANLCILDPLKNPLEVLTVSNAVGTQKSN
                     AILHLLYDTKPTQGHSADRKHRLEKMILSVLKLASSEHRAPWEKVRDLFVSKGETTRF
                     EAFSLRTFVEEIAPAGKYDNLSEREKGLFKSLQTHPDFIEFKWEWFITDIYERAFYSY
                     DARKIVELFDLPYLPLV"
     gene            3404..3679
                     /locus_tag="PKM49_00010"
     CDS             3404..3679
                     /locus_tag="PKM49_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81228.1"
                     /translation="MPVYTQCNTDSELNALFIFCDIQEITEAFEAIKKMRFFAKIVPT
                     PKDVFPAKSLSIAVKKEDLGILSDKLQELKIVPIKSVTFEKCVFSYP"
     gene            3696..4349
                     /gene="ruvX"
                     /locus_tag="PKM49_00015"
     CDS             3696..4349
                     /gene="ruvX"
                     /locus_tag="PKM49_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNY81229.1"
                     /translation="MILACDYGKKRIGIAFADTESGVIVRSGVLEQTKSAVERIRRIV
                     QEKSVQKIIVGLPLNLRCSFTESTDAAVSFASSLFDRVSRAVFFIDERFTTASVYVTG
                     RLLGKSEAESRIAVDAGSAISLLDTYLKNPKTALPFSRKTLSPEDVRRMIEERTSQGS
                     FGDKPIELVINGLSRFSPFSDTQRLWALREQNPWHFSKYRAIEKPEAIVEYSFHYGH"
     gene            4350..4426
                     /locus_tag="PKM49_00020"
     tRNA            4350..4426
                     /locus_tag="PKM49_00020"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4384..4386,aa:Arg,seq:ccg)
     gene            4445..5122
                     /locus_tag="PKM49_00025"
     CDS             4445..5122
                     /locus_tag="PKM49_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HNY81230.1"
                     /translation="MILIESAFKDELEPIVQQLVVFEEGKVFDRDYTRGVIGRNEIVA
                     CSGMTGKVETSVMTQKLIELFHPACVFFVSCAVGLAPDLNPGDIVLGEAYREHDRPSL
                     DDTLLPLITGNIEFAYRLRQEYPHLKSGVIISGDTIPTDAQNRRALYLTHRASAADMH
                     SAAAAIVCRMNGIGFLSFQTIVGRTDFPSLEAYDRQRRTHAAAAATFLASYIEKNFLS
                     ISCAEKK"
     gene            5179..6162
                     /locus_tag="PKM49_00030"
     CDS             5179..6162
                     /locus_tag="PKM49_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNY81231.1"
                     /translation="MDHTGLESEIDSLRPQIRSVFSELHRVIVGQEEMLEGIMIALMT
                     KGNLLIEGLPGLAKSLAVSTYAKISELSFHRIQFTPDLLPGDITGTLIFNMRNETFVV
                     EKGPIFAHFILADEINRAPAKVQSALLEAMQEKQVSIGKTTYPLPKPFLVIATQNPIE
                     QEGTYPLPEAQVDRFLLKLEINYPTFNEEKAILEQKGVDQDIPLNPCLSLPLIETLQR
                     VTDEVYVDEKLVTYIAKIIYATREPTRYKLDELIPFIRYGASPRGSLSLLKASKAYAV
                     IQGRAFVTADDIKRIAYPALRHRILLTFEAEADGLQTEEIISRILQGLPVP"
     gene            6159..7076
                     /locus_tag="PKM49_00035"
     CDS             6159..7076
                     /locus_tag="PKM49_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNY81232.1"
                     /translation="MKRQDPEPSAEFLEKRKEILRRIRLLRLFAERSISSQTLGIYRS
                     FFPGTGMDFSEIRGYYFGDDIRNISWPTTAKNRKLYVKSFKEDRSLNVVIAVDCSRSM
                     GFWSKTRRQENMAYIAACLAFSALKAGDKAGLVLFSDRIVDYLPAKNAMRNGVVIIDA
                     VLRANPSGIADFSVLHGFLLSILKEPSAIFLISDFFLSEPEIDDLVAKLRVLRVRHRV
                     YPFSFWDQAEFVLPELGYARVRDMETQRTEWCKISEPLIEDYHRQMEKKRTYLRESFE
                     KAGILYLPLLEGEDYGKRLFDFFKKKPPL"
     gene            7141..8079
                     /locus_tag="PKM49_00040"
     CDS             7141..8079
                     /locus_tag="PKM49_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81233.1"
                     /translation="MPYAVAAAGGPPTYPVIGTRVRTFAAYCDQTEVTLGETFRVVVT
                     ARYFPHELVTCGGLEALHESLALTGVSTVSHLLAVNYVEETWIYTLTPTTAGAFLFPP
                     LYASFWEPGTPANQAVKLSSRPLYLDVYALAAPYGFPEGELKKVAPISPWARWIGWLI
                     SLGLLIPCGTILRKAFVRPKPAKAQPEKPEGLEALELLEELKKEYYLLLKDSKTINTP
                     EDVKEFYYRLSHFVRGYFDAAYQTQMSMKTPLEIRSVFSGTKEIEMKTLEEVLSFLVR
                     CDSVKYRPLAPLSEPIDSDIRWLKKITASISKRKIG"
     gene            8090..9064
                     /locus_tag="PKM49_00045"
     CDS             8090..9064
                     /locus_tag="PKM49_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY81234.1"
                     /translation="MFRFLIPTAFLFFIIVPIALWAAYKRNKRKVFSFSNADMLFALK
                     KPKATVEKCSWFLKIAALILFVFACARPVQTRDVSLFTQQASLDLLFTIDISSSMNET
                     ATANTSDKSGLARIEYAKQALYALLDITERERAGLVVFAQNPYTVLPLSFDHTPLRYY
                     ASRLQDYPGTIEDGTAVWDSLMVSAERLGTVDSPAKVIILITDGANNCGFFTEDQVLE
                     RLLRDDIRLYVVHVGNYFSFTLTTRGEIDKIRKVSERSSGVFFTFSEERNIQKIAKQI
                     ESLERSIEEKQERLRYHQDYYLYLLVPALVCLSVSLNLDWIYRKGYLL"
     gene            9061..10071
                     /locus_tag="PKM49_00050"
     CDS             9061..10071
                     /locus_tag="PKM49_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY81235.1"
                     /translation="MKFANPSYFLLLFGLIPYAFFLFLRYRKAEKRRRADFSAEAIKE
                     LYPSRSIAAFFVRNLFMLAALLFALIALTNPYTRESLISAPRPVKDVMIALDVSLSMN
                     ARDVFPSRFEAARRELLQVLKVPNLRASLLLFSGKTLLTLPFTQDRQALSLFAQELQP
                     GYIRSEGTNYQALFETIIEEYDHSLSLSKELGWITSRAPTHVIVLTDGDGIEWPEEPL
                     FYEMRKRDIILWVEAFGSVEGAAIPLYNENGEFTDYLTLNGVRYHSKNQEALLRNWVE
                     STRGYYVSYSTTERLSEKVFQIQDKMGTDAVFQDYLAIKNTVFPAFLALAILSLLIGM
                     VL"
     gene            10068..10919
                     /locus_tag="PKM49_00055"
     CDS             10068..10919
                     /locus_tag="PKM49_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY81236.1"
                     /translation="MRRMDRRLKGFLFALIGMLSVALLLSSRPIPLDPTLPRSVLKAA
                     ENPRDIVSNNDAAADYYLLGERNLARVYIERALQEGNPDDGHRDELLTNYGDILYQLG
                     YLEESLEAYQTALYLNPINERASTHYEIAYNRYLRHLETQISEEGGIESERTEEREGE
                     ESGDAEGERGQESDQNKKESEKGSKPQEETGQSSSDDPEENPEQKEHKGKRQDKQDKA
                     EDENVSGTGQQAEGSTNPESDQREHSVGFATLEDYYRKINQDDTRQIYKFLFPDEGDH
                     GEKPRLP"
     gene            10894..12954
                     /locus_tag="PKM49_00060"
     CDS             10894..12954
                     /locus_tag="PKM49_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81237.1"
                     /translation="MEKNRVCPRLKGILVFIVLYALSFVLGFALQLSISVDPPFQQIE
                     RGTPFLIRYVIFDYEGEEKNLTVSLDTQAAFSGNFDEFLASVIDHAIKPFQELGSIDR
                     ELEDGAVSGGIICSSALVGTHELEVLVFEKDRLVKSESLVITVTENHRLSQALNARWE
                     KKKKPEHFGLSFLSENAVFYNEQYSFSMIAVEKVSASETLKLSGFYYYTPALSQAKAL
                     RYAAYLTETLGGETYALKMADVVVNPNPLLVEQAKVKVYHPYLRWIDASGKEIEDLSY
                     EDEYETVLYQKPTDSSFFQADPIVLKEWEASPALQTGEIGTVRFSIESRSLFFGSTFK
                     MISMPSAQTSLTLRRSWITESEGIRRIDRTTHYEYDFLPESAATYTLTIEPFAYTVTR
                     TGETRVFQTDPLTIVVTQRLEDKPFTGILNPLKPLRENSARVKNKQEFEKGLLYALEE
                     NWEEATRVFSRLAEEEPLSDDIWYNLGWVAFSSDQVGLSQLCFYRAFTIVETEDTQYN
                     IRFIETAKGLSGRPLAKVKRIDGLAHRWLGVGGVVCLALFSFSITLRLRRLSVFKRRR
                     EAEHPEKHRRLSRALLAFIALICLAFCAANLLREYAFERQAVVLFESPAYGGPAFDYP
                     AIGTIAPGNVGKCLAQRDGFSYMEYLAPWAIDAPFSGDVTSAVRYWVPSENLEVILST
                     LFTE"
     gene            13196..13657
                     /locus_tag="PKM49_00065"
     CDS             13196..13657
                     /locus_tag="PKM49_00065"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY81238.1"
                     /translation="MDSFNGQDTLERTGSAVYEDFYLREDRGKPAINARIALGAFIVQ
                     TLLKTTDEETVEHIEEIPYIQCFLGLGAFTTEPIIASSSLSQIRERLSEEDRQAINEM
                     LMDAIKGEENHWKTEKEEELVVEESIKREMDRMREVITERFRLWRYGPAED"
     gene            13819..14130
                     /gene="rplU"
                     /locus_tag="PKM49_00070"
     CDS             13819..14130
                     /gene="rplU"
                     /locus_tag="PKM49_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNY81239.1"
                     /translation="MYAIIHASGKQYRVEEGQVLYTEKQPFEKDERIVFDRVLMVKDE
                     GVTIGKPYIEGAKVIGTVQEHVREPKVLIIRFKGRKQYRRTRGHKQWKTAILIDKIER
                     A"
     gene            14147..14458
                     /locus_tag="PKM49_00075"
     CDS             14147..14458
                     /locus_tag="PKM49_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HNY81240.1"
                     /translation="MICCTVFRTATGYRGFEVTGHAGMARKGKDVLCAAVSILTTHTA
                     MTIQEELGKKGTGKQTAGYFRFEITDTDETTDMLFRAFLKRMIGLSEEYPRKIKVEVK
                     T"
     gene            14461..14769
                     /gene="rpmA"
                     /locus_tag="PKM49_00080"
     CDS             14461..14769
                     /gene="rpmA"
                     /locus_tag="PKM49_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNY81241.1"
                     /translation="MHLDIQLFGHRKSGGGKNGRDGNPKYLGVKKGDGSKVTPGNIIL
                     RQRGTKIYPGLNVGIGRDFTLFALAEGVVRFKDKHKRKYANVIVDTPADTAATAVSES
                     "
     gene            14845..15285
                     /gene="rplM"
                     /locus_tag="PKM49_00085"
     CDS             14845..15285
                     /gene="rplM"
                     /locus_tag="PKM49_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNY81242.1"
                     /translation="MIYEQKSFRLKQEEITHPWYVIDADNQILGRLATRVSILLTGKN
                     DPRYTPGVDSGAFVVIVNAEKIQMTGQKWDQKIYVRHSKYPGNTKELAAKDALKKKPG
                     FIIEKAVKRMLPKNRTGLKMIKRLKVYAGAEHPHQAQKPVEITV"
     gene            15307..15708
                     /gene="rpsI"
                     /locus_tag="PKM49_00090"
     CDS             15307..15708
                     /gene="rpsI"
                     /locus_tag="PKM49_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNY81243.1"
                     /translation="MENPDYYGTGRRKTSVARVHLRPGTGKIKLNGKPLTALKDYLLR
                     DSLVIEAMKPLTLTGLADRFDLVLKLSGGGLSGQAGALKMGIARALLEYNNDLRPTLK
                     AAMLLQRDSRMVERKKYGLKKARRAPQYSKR"
     gene            15847..17748
                     /gene="dnaG"
                     /locus_tag="PKM49_00095"
     CDS             15847..17748
                     /gene="dnaG"
                     /locus_tag="PKM49_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNY81244.1"
                     /translation="MELKEIVREVKDAVSIVDLISSYQIQIQRSGKYYKALCPFHGEK
                     TPSFFIYPEKKRYHCFGCGATGDAIKFVQEYEKITFIDALKELASRYGVSIQSLSDRT
                     NPLKQLYVDTFETLAKQYHLSLLHHPNALKYLEDRGYGKSEAQQFQIGYCDLRFPMKS
                     FQQWLKGIGSEPENEQRWKEWGLINQDGSSRFYRRIIFPIRDRYGACIGFSGRLLERA
                     EDQLDPTEKKDSGFDAPKYLNSPQTPFFNKSKVLYLEHVARKPIQEFGFAIVVEGFFD
                     AIRLHANGISNTVAAMGTAFGAEHFRVLDRLTKQYLFIFDSDEAGKKAAERAFFSLPS
                     EVRAMGCFLSEAKDPDEYLRRIGKDRFIQEMAAAKPLEEFLIDFLKDRFEITQSVGQD
                     EYLRASLPILTKVKEIGNLPSYEKVVGIVAETLGIERPKVESWYREHITKTATTKHTP
                     EKTGSVPIEPREKTGTEKTETRALSTGNRLSRWAGEENPAEEHILAIYLERSSFRKEI
                     ETFVQEHTAYLNDDFCSFFDKARKEFEPDDATNHLNEFFPKEFLHRVFRFMNRPFFIK
                     DDPARISKALGDCFGIIRARREVSQMKNLERQIRDTTDLEEKKRLMKQSVELIGASKK
                     KSKIEGRPF"
     gene            17982..19079
                     /gene="rpoD"
                     /locus_tag="PKM49_00100"
     CDS             17982..19079
                     /gene="rpoD"
                     /locus_tag="PKM49_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNY81245.1"
                     /translation="MTELATIGSKKGYITYEDIDAYLPAEMEGFDSNFLEMVYETLEK
                     NKIKIIEKEPEVDESGSEEPVEDELVERMISEDGPAIFDNNSFNKDGVKMYLKEIGRI
                     PLLTPTQERTLARKAQIGDERAKQKLVVANLRLVVSIAKRYIGRGLSFLDLIQEGNIG
                     LLKAVEKFDWKKGYKFSTYATWWIRQAITRAIADQARTIRVPVHMVETINKINKIRRE
                     YLQEYGNYPTTNELATLTGRSMEKIEEILQADKETVSLESPVGGDEDSSVGDFVPDNE
                     MINPEESAMLMLLRDEITEVLNTLGPREATVLKMRFGLIDGRQKTLEEVGKYLNVTRE
                     RVRQIEVKALRKLRHPTRSKHLKTLMNYLNK"
     gene            19146..19826
                     /locus_tag="PKM49_00105"
     CDS             19146..19826
                     /locus_tag="PKM49_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNY81246.1"
                     /translation="MEYYSSMLIVIPAIIFAIFAQSAVSTTFSKYSQVRSETGYTGKQ
                     LARTLLDSHGLSAIAIEPVPGVLSDHFDPRQRVVRLSESTFQNGSVAALGVVAHEVGH
                     VIQMQERYRPMVLRNAVVPIAQFGSSFSWLIFFFGLFLSIPALVQVGIGLFLAVVAFS
                     LVTLPVEFNASRRALTLLQTHLLMSQEELSMVKKVLRAAAMTYLASTLMAVLNLVRML
                     MISKRSGD"
     gene            19832..20245
                     /locus_tag="PKM49_00110"
     CDS             19832..20245
                     /locus_tag="PKM49_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81247.1"
                     /translation="MGIETTLSNLVGQSQNYQFRVKESDLWQEDPWVLDLKVLSSRAL
                     IHRLFEFSHQFLIEHVFGAELAFAVTDLEYRHLAPTPLGSAITVRLTVDYTLRNHVYF
                     QFVAFDEHEEIANGRISRVVLSKDFIGRNIQHKLL"
     gene            20242..21777
                     /locus_tag="PKM49_00115"
     CDS             20242..21777
                     /locus_tag="PKM49_00115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81248.1"
                     /translation="MRKPRNSADFAEFSTVKAFSEEETQYDRHKGYGDVDVALIFPSD
                     YDQTVGNLGYHKVFQILNTVEGVNCERFYYDPSFTKFYSLDSFRPIDEFSIWAFSIHF
                     ELDIFNIVEMLRKKGIPLRSVERRGEHPFILIGGSLTYFNACPLWELAGIILYGDAED
                     PLPTLVKTIVPAVVKGKRRQEVLETLSEMETVALPPLEKEHRTLSVFSSLSRFPSRSS
                     FVHPKGPFGRKALIEVGRGCLRRCSFCVTGFTRSPARFLPFDILKKEIEDLIDKEQAA
                     CGKAPAFGFISATPTDYPHLPELLAFLERSSLDFGFSSIRLESISDALLSGLRRGDQQ
                     SITIAPEAATERIRRVLNKGITDGQLADALERIARAGFREVKCYCLFGIEEETEEDLL
                     AFARLVEEWRKLGFYKIHLSFNPLIPKPNTPFESRVLESEGTLRRKKKLLENALRGLC
                     QTQFESLRNSQLQSLLAKGDRSLSKHLVSGHSGQTIQKQLLQNVRTQTRTKDKGSSHA
                     TIR"
     gene            21849..23354
                     /locus_tag="PKM49_00120"
     CDS             21849..23354
                     /locus_tag="PKM49_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81249.1"
                     /translation="MFPKPRFWIVVILLSLWLELSLFALPQSHSVQIAIPSDPADTID
                     PHRAAGALTFEILYNLYEALLEVDPAGQLIPVLARSYTVSEDGREYRFLLKDNVVFHN
                     GKSFSAADVVYSFRRILDPQTGYPKTSNYQMIEAVFAEGPDTVVFRLKQNYAPFLALV
                     SKIYIVPDGATKEQLDSAAIGTGPFVLKEWARDQYLHLVKNERYHQEGLPYLEEAYFR
                     IIPDENSRLIALKSGAIDVIPKADTSSLYEFVGNPAYKVLEAPMNLVQVLALNNKKPP
                     FEKLEVRQALQYAIDRDLCIELVAEGLAKPLYTHLPPEDYYYRDFSAAYTYNPAKAKE
                     LLKQAGYPNGCPFTIQIAQLYPFHIRTGEVIIQMLNEAGFKAKLQVIEWSKWLSDVYG
                     AREYQATLIGHVGEADPYLLLDRFYQTSNRNYMNVFIPEIDQLLDASLVVSEPEKRLP
                     FIHRILKILVENAVAYWIMEPEEIVILSSKVSGWVIYPVYVDALKRVQKAP"
     gene            complement(23376..24014)
                     /gene="nadD"
                     /locus_tag="PKM49_00125"
     CDS             complement(23376..24014)
                     /gene="nadD"
                     /locus_tag="PKM49_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HNY81250.1"
                     /translation="MRPNDVYLGGSFNPIHHGHILLVQRLLDYVWVRRCLIVPTGASP
                     FKEGLGTNQPYAVRLRWVEKAFEGIERVSVLKLESPYRDEEGRMRSVFYTNETLDRFY
                     RLYGYYPYLGIGSDALASLHHWKDWQKLLEKTQVVVFGRSAGLAPPHNEEWASTYCQR
                     IHFLDTPLCDLSSTDIRNRAILGKSLRGFVPEPIEREVAEAYRLGPLRDTCP"
     gene            complement(24016..25131)
                     /locus_tag="PKM49_00130"
     CDS             complement(24016..25131)
                     /locus_tag="PKM49_00130"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81251.1"
                     /translation="MGKVFFPWLLKHPKYFRQAFTLLKAFEKAEEKRAQQLARGLLIP
                     PVVILSITSGCNLSCEGCFACATGTVSQPQEAGGNAGPGPSLDYTQWKRIIAEASQAG
                     VFCFLIAGGEPFLFPETFQLVRDFPHLLFVVFTNGTMMSDSILQDLKKAHNLLVAVSL
                     EGDETLTDLRRGKGTYRKAMDTLRILEKNGVLNGISVTINRLNYGFWAKEEHIDRMIE
                     SGVRIAFLTEMIPVSGGEEDYPDTSYGMAYATMLTDEERSTFRQRVLYYREHKNLFII
                     HSPGDEEAFGGCVSAGKGFIHITPYGDVTPCPVSNIATDNLKTCSFENAMRSELFKRI
                     REEEHLLETGNGPCALFSHQKEVEALRKEVGAYRTGT"
     gene            complement(25850..27475)
                     /locus_tag="PKM49_00135"
     CDS             complement(25850..27475)
                     /locus_tag="PKM49_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HNY81252.1"
                     /translation="MFPFNVTQNRRPKIQYPVFGSNGIVATSQYLAAQAGLRMLEQGG
                     NAIDAAVATAIALTVVEPTSNGIGGDAFAIVLFEDQLYGLNSSGYAPASLSLEVLKRE
                     GLQEIPRYGPIPVTVPGAPRAWKELHSRFGVLPFDALFQPAIEYAQNGYPVSVLTGKG
                     WEKAFQTYRQQPSTSLFGEWFRTFAPSGRAPFPGELWSSNAHARTLKELAETGTDSFY
                     TGRLSREIVRCVRSFGGFLESSDLADYQAEWVEPLSVDFRGYTVWELPPNGQGIVALM
                     ALGFLEPFSLETLSEVQRTHLSIEAVKLAFKIATETIGEPRSMPKDARSYIKKAALEA
                     GAKTLSLDSVSDTTASSLPGGTVYLCAADRSGNMVSYIQSNYMGFGSGLVVPGTGISL
                     HNRGCNFSLQSGHPNCLRGKHRPYHTIIPGFLSRGREWIGPFGVMGGFMQPQGHTQLL
                     IRLLVEKAHPMDALNAPRWQWLKDRTVAFEEPYDESIRKELARFGHYIDTVPTSALFG
                     RGQVIWRSAKDSTGRYVFCGGTDGRSDGSVAVF"
     gene            complement(27769..28839)
                     /locus_tag="PKM49_00140"
     CDS             complement(27769..28839)
                     /locus_tag="PKM49_00140"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNY81253.1"
                     /translation="MLEWKRTSEIRAGDVIAETLTEYETGETIVHMGETVSVSIKERL
                     VRHGIEWILTESRETYKSPIKPILSPEVLEEMEKGVKKVYDAVQREQKIPVEEVSTLS
                     RQVVRNMLSNYGDVVVPFFSQMRTFDEYTFCHQVNVSIIAGLIAIEVYSGDRETAEAI
                     VAGALIHDIGKLVVPKELLNKRTSLADNEFELMKKHVLYGHQMALDSGIDNPIILHEI
                     LYHHERIDGSGYLSGLKGREIPQSARIIVIADVFDALTSDRVYKMRWSPYQAIGYIIQ
                     QAGKIFDPYIASCFLRLFGIYPVGTRLLLSNGREVVVKGNRKNALTTPIVADIEQEEE
                     LIYLDRQKGLFVERVLYDHKED"
     gene            29153..31231
                     /locus_tag="PKM49_00145"
     CDS             29153..31231
                     /locus_tag="PKM49_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY81254.1"
                     /translation="MEYEQIKALYRLAKEFLSAEPVDLLRTLKKSVRIIHSLFPRCSL
                     WLWVIGREREPWTCYWSHCHTVGDQSRWPDGLEGPLSNGERGGEKAADRPGIRIISEG
                     KTKARVFIPLEHQRRALGFLEILHNKTVRIEEGEWMAFYRELSSIFSGVLLRLNRAPA
                     EEIDLSRIRLPGMIWETEPDGRYRYVGPRSEELYGYLPEEMIGQPRQDFFEGPSETIP
                     SDLEPPIVPRTNEMCSKYARVKRKGGKWQQIIASEMPLFDESGKWSGFRGIDHIYPEP
                     SSTGPFPGVNQELERRVTERTQAFQRMIERYKTVQMRLEMVLWAANQGIWDWDLQTDT
                     IYHNENYYTLLGFSKRDFPDPNKVWRQLTHPDDIDLVLKALDDHYSGSKPYYEVEYRM
                     RKKNGEYAWILDKGRIVSYGENLVPLRMVGVHSDITERRAFEEELRQAKQAAEDANRS
                     KSEFIANVNHELRTPLTVIMGLAETLLMEMPDNKKEFPIHILKNAKQLLLLINDIIDL
                     SKIESGKFSLAKKSFQLAETLDYIRQSFALETEKKGISLKILGPSIVLYGDHVRLKQI
                     LTNLVANAIKFTSQGGVTLTCEISSEEESKVVLLFEVQDTGIGIGESELNKIFERFYQ
                     IDGSNHRKYSGTGLGLSIVKQLVSLMNGSVWVKSQIGEGTTFYVRIPFALPNRPLKPS
                     TNTEREVTTE"
     gene            31445..31717
                     /locus_tag="PKM49_00150"
     CDS             31445..31717
                     /locus_tag="PKM49_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNY81255.1"
                     /translation="MNKKEMVDKLALETGVTKKEAAQFLDAFVDVVSNELSNGGNVRL
                     VGFGTFMVKNTVERNGVDPRTKKPIKIPARKVPKFTAGKDLKLRVK"
     gene            31787..31861
                     /locus_tag="PKM49_00155"
     tRNA            31787..31861
                     /locus_tag="PKM49_00155"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31819..31821,aa:Val,seq:cac)
     gene            complement(32705..34291)
                     /locus_tag="PKM49_00160"
     CDS             complement(32705..34291)
                     /locus_tag="PKM49_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81256.1"
                     /translation="MGLGKSMKDKVSSIAIGTIIDQAVKYIHKDPQNNFEKITANLGK
                     LESLFSSSGGKFTKFLGWANANPGSKQWFIELLSRDENQVRTFMKNMFVNISLGWMDN
                     NEELMEKHGITAPYTILISPTMRCNLRCQGCYAGEYAQQGDLSADELRRIIKEAKSLG
                     TFFFTILGGEPFVRFWELADIFEDNSDCLFQVFTNGTLITEEVADRLKELKNVVVAFS
                     VNGDAKETEFMRGPGVYEKVLASMKLMKERNLLFGMSLVLTKHNFDIMTNPEFYLGWR
                     DRGVIYAWNFLFMPVGKDASVDLMPTPEQRYRYGEFIQKFRSEQPFYIMDFWADAPFV
                     HGCIAGGRRYIHINHKGDVEPCIFAHFATDNIRDKSLVKCLQSPFFADIRMHQPHTDN
                     LLRPCMIIDNPEVLRSVVKKHAARPTHEGAEVIFTQLADDLDAYSASAAEYLDPIWEE
                     SWQKYILDMETRGASYGEGFDRLQYRLGLEQFNERIENLKKNDPEFAQLLADLAESAS
                     EEYGKDPVRQRKMIGEIEVK"
     gene            complement(34421..35461)
                     /locus_tag="PKM49_00165"
     CDS             complement(34421..35461)
                     /locus_tag="PKM49_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNY81257.1"
                     /translation="MSQNLSQQNLSADPPGSPDEEQKGYTGMDLRRLSKRNRLFVSFV
                     IFILVCIVAYMVLRTQTPYSDHAFSVQGRAVRLVLGSQGDHETLLRAAQLELERVFNR
                     FSFFSPESETTRLNRISETNRKTDAETYAFLTKLEDLSEKTLWHYDFTIGIHYRPYAL
                     FELPTLETYRYPTDLNLEDGSVHSDHPFLFFFGKAVKGYALDCVYESIRKTDPKATGY
                     CEIDGDIRFFGPKAGGADWIAGGWNNQELYLSSGALFHHPTLTPPEGTAAYEDHLLLS
                     PDKGEPIKPIEYAAVIAPTALEAACFVVAAQTLDSEILFQTLSRIGAAAYLITDGKAH
                     YSTRWTLYTYVR"
     gene            complement(35471..37018)
                     /locus_tag="PKM49_00170"
     CDS             complement(35471..37018)
                     /locus_tag="PKM49_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81258.1"
                     /translation="MDKQKKNTKENTKENLTVDQVGESFEVEFLPRRVLLWRSFKKHK
                     LGILSMWILAFFYLIVIFADFLSPMSPYKQNASIGFAAPSKIYWKHKGEWIGPYIFPL
                     ETARDPITYQRSFYETSELRMVKGVGTNGSPFVYTLGENNVTALVAQVKIDEIGVGAT
                     GEGLFKTTTKDWNMVRIEKEGIFKEPLVNVSVEKDTLDLYRSNPRYVKTLQQIGDLAQ
                     IKTVESLQKISVKYADGKTESISLIALEEYDFKVYPIKWFVRSWEYKLFGFIPSNLHL
                     FGVDSPAVLYLFGGDNYGRDIYTRILFGGRTSLSVGLIGILITFTIGLFLGGISGYYG
                     GWADEILMRLTEILISIPTLYLLISLRAILPPDLPSTTTYLMVVVILSFIGWPGMSRV
                     IRGMVLSLKQKEFVEAAKAMGYPSLRIIWRHIIPNTATYIIVAASLSIPGYILGEAGL
                     SFLGFGIREPQSSWGLMLAQAQDLVALRNYTWLLLPGLFLFIAILAFNLFGDALRDAF
                     DPRSLGH"
     gene            complement(37029..38000)
                     /locus_tag="PKM49_00175"
     CDS             complement(37029..38000)
                     /locus_tag="PKM49_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81259.1"
                     /translation="MLKFIIRRLILSIPVLLGVSILSFLIISAAPGNFLDVFRLDPSI
                     SRETVQRLEREFGFDKDPFTQYLTWLFQVVKGNWGYSFQFKQPVFNLIWQRLGATLLL
                     SLTTLVFSWAIGIPLGIYAALKQYSFKDQLSSVIAFLGLSIPSFFFSLLFLYFASRTR
                     LFPIGGMLSVNSSQMNTWELIVDYLWHVIGPAVTLGFSGLAGIMRQMRGQFLDQLHMD
                     YVEFAKAKGMPRKVVFYKHALRNAINPLITIFGYSLSGLLGGAVLTETVFGWPGMGRL
                     VIQALTAQDLYLVMASLMVSSLMLIIGNLAADILLALVDPRIRYRFH"
     gene            complement(38061..39800)
                     /locus_tag="PKM49_00180"
     CDS             complement(38061..39800)
                     /locus_tag="PKM49_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81260.1"
                     /translation="MKKAFSVLLVLCFLLGIVFAGIVPSENLQYIVEDVHGKVGGTLY
                     YYSISDPKTFNPAFAQETSSTDITGIMWDSLVDFGKNDDIRGGNLALDWEVTPEGNGW
                     TFTIRKGLQWSDGHPLTAEDVYWTFKNIWFVPENCLSTYDVITDAQGRPPQIEKLDEN
                     RVKIWYREPYAVGLRQIGGVQVFPKHIVEPVLAAGKWKEFWTLADVNKLVGSGPMLVS
                     EYLPDQRVVLKRNPYYFRVDPAGNRLPYFDTLIFISVPNLNTAQLKFEAGELDYLAPQ
                     AAQYPNLKAQAAAKKWDVGICGPTYSTLFLAFNQHSPTKEQQYWFRNKKFRNALSCAI
                     DRETMIEVIYNGLAQPQWGPVSPASPFYDPAVEDLAATFDIAKSRAILKEAGFTWNAE
                     GKLIDDRGIQVKFLLTTNAGNNQRETACNLMVDTFGQIGIDVIFNPIDFNTLVGALTA
                     TGNWETMVMGLTGGPDPHSGSNVNRIDGDLHFWNIHPDVGTWADPDNYWISNEEWQID
                     RIFRDQVREMDEEARWQMFADYQRLFVEEAPLVYTVQALRLYAKKAELKGTDPGSSSS
                     PPILWNLWGLYRE"
     gene            complement(39850..40869)
                     /locus_tag="PKM49_00185"
     CDS             complement(39850..40869)
                     /locus_tag="PKM49_00185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNY81261.1"
                     /translation="MPETGSTALLEVKDLRKYFPIKSGFLIQRTVAQLKAVDGVSFAI
                     DKGRTFGIVGESGCGKTTIGRTIIRLLNPTGGHILLEGQDIATLKERPLKDYRKKMQI
                     VFQDPTSSLNPRMTVGQTLSEPLLFHEMVHSKKEAEERMSQLLSSVGLKPYHVDRYPH
                     QFSGGQRQRIAIARAICVDPSLIVLDEPTSALDVSVQAQIIKLLKELQGKLNAGYIFI
                     SHNLSVVRYISDQVGIMYLGKMVEKGDTNEIFDKPEHPYSKALLAATPIPDPKTRRKR
                     KELLTGQVPSPINRPSGCFFRTRCKYAMPACEKEYPEMVRIAEHHWVACHIYSLGEPD
                     RIVEK"
     gene            complement(40862..41896)
                     /locus_tag="PKM49_00190"
     CDS             complement(40862..41896)
                     /locus_tag="PKM49_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81262.1"
                     /translation="MNEPILKVEELRTYFDLTEGTVKAVDDVSFSLSENEVIGIVGET
                     GSGKSVTAKSLVGLIAKPGRLAGGRVFFKTPEFPERQKDGFLDLAQLQGDEYTKIRGK
                     HISIIFQDPMTSLDPLYTVADQMTETIMFHQKVNEKEALDIAVEMLDKVGFSNPRERV
                     HQYPFELSGGQRQRVVIAIAISCNPEILIADEPTTALDVTVQAQILELMKDLQAEFRS
                     GLIFITHDLAVIAEMSDRVMVMYGGKTMEYTDVYTIFENPMHPYTHALLSCIPRMDRR
                     DQVLEPIPGQPPMMLNAPNLCPFLPRCQRKIERCLKELPSLIRMEDHYIRCFNPIERS
                     SASRRGGKHA"
     gene            42258..44639
                     /gene="leuS"
                     /locus_tag="PKM49_00195"
     CDS             42258..44639
                     /gene="leuS"
                     /locus_tag="PKM49_00195"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNY81263.1"
                     /translation="MYDFRAIEKKWQDIYAAEEPFRIDLEGAKKPFYNLMMFPYPSAS
                     GLHIGNMFSFIGSDVYGRYMKLLGYDVFEPIGFDAFGIHSENYALKTGRHPAELTPES
                     IRFFREEQLKKIGAIYDWRSEVNTSAPEYYRWTQWIFLKLFEAGLAEKRESEVNWCPS
                     CKTVLADEQVIEGQCERCNATIVKKTMSQWFFKITKYADRLLENLEKIDWTEITKNIQ
                     KSWIGRSKGTTISFPIESTDEQIEVFTTRADTVFGVTFLTLAPEHPLVERIVQEERRE
                     AYQEFLSQVAKMDDATRTSISRSKTGIFTGAYARHPFTNEKIPIWVGDYVLYAYGTGA
                     VMGVPAHDERDFLFAKKYDLTIRKVIACSDDQLPYTEKGEMVHSGEYSGWASERFISQ
                     AETLAPFLKSKVQYHLHDWCLSRQRYWGPPIPMLYCENCGTVPVPEKDLPVLLPVTDD
                     YIPDGSGLSPLARHEAFLWATCPVCGGKARRETDVSDNFFDSAWYFLRYVSPDDSKKA
                     FNSERVQKWLPVDMYVGGNEHANLHLMYARFITMALHDIGLLPFEEPFTKFRGHGMII
                     KDGQKMSKSKGNTVNPNDYFETHGVDTLRTYLMFMGNFLEGGDFRESGLDAVKRFIGR
                     VWDLTALPSGPSSLKAKIKMAETIKRVQFAFENIKYNTGVAALMELVNSLQDEQALEP
                     SLIVDIARLLAPYAPFISEEIHAMYGGKGTILNTRFPTGYDVYLEQALAEIPVQINGK
                     LRGKITIAQNATQEQVMEKVLEDGRLTAYFQGREVKKVIYLQDKIINLILGSM"
     gene            44822..46027
                     /gene="folP"
                     /locus_tag="PKM49_00200"
     CDS             44822..46027
                     /gene="folP"
                     /locus_tag="PKM49_00200"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNY81264.1"
                     /translation="MLFKRFLCSDFRENAERFFVTEDTYALCAYRLSQQERCLLLDIC
                     LPYLTALNLSEIRIPMNGMPNEGFLFLGERPRWEQTIWKLLRSRSDLTIVVLTQILEL
                     LYNRNLRPITLPSGKQLSFERPLFMGVINRTTDSFYAQSRIESDQEALEKALEMEEQG
                     AQLIDVGGESSRPGSLGIPEQEEEERVIPAIRLLAKHIKIPLSVDTTKASIAKKAIDA
                     GADIVNDISGLSPDSELFEYVANQKIPAVIMHIQGTPKTMQTRPTYRHVVREVQGRFE
                     LVLNALGERGYPLHQAILDPGFGFGKTLPDHLYLWSELASFLRFRLPVLVGTSRKSFL
                     GATTGTSTPAERLPETLATTASGIARSVNIFRVHDVKENFQVAKMAFFMMGFVCEGVN
                     ERGVHSETR"
     gene            46014..47519
                     /locus_tag="PKM49_00205"
     CDS             46014..47519
                     /locus_tag="PKM49_00205"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNY81265.1"
                     /translation="MKLAEVKAVLEKNGLLLSIANYQGNENLELDSVSIDTRNIVPET
                     LFICRKGVKFDSHALVPEAVRLGAKALIVEKPVDADQIPMLLVSDSRYAEAVVSSAFF
                     DFPHEKLRVYGITGTNGKTTITTLIHYVLTRKGKKGSLLGTVKNIIVDRYYGSEHTTP
                     GALEVLKETHETIAKGGEFLSMEVSSHALSMKRVESIRFDYAILSNITQDHLDFHKTM
                     GDYANAKYHLFELLKADGKAILNLDDEWSTKFASRLPLDKTILSYGLYNEEKMPDYAA
                     KNPKVTIEGLSFELWVKRVKVGTVRSQLIAEYNVHNLLSVIALLHREGLSFDEISAYI
                     SEFRGVEGRFELYPHVGNDIDLVIDFAHTPDALEKTLGTARALTRNRLIVVFGAGGDA
                     DQSKRPIMGQVVAERGDVLIITTDNPKSEKPFDIIRQIESGIRTDVPYLLVEDRKMAI
                     ETAINIASKGDMIILAGKGHEHYQIFDHAYLPFSDREIAFQMIRTLKKSAS"
     gene            47545..49083
                     /locus_tag="PKM49_00210"
     CDS             47545..49083
                     /locus_tag="PKM49_00210"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNY81266.1"
                     /translation="MIFTEWIELLSAPGLRNKAILEQLFNPSEETARGIELRFCHDSR
                     EARAGDIYIAFPGERVDGHDYVDEAFLAGARLAIVSEIRPYNGHCLYVEDTRRFVQEL
                     AARWWKTLNPKGIGITGSNGKTTTKEWVKDILNQFLPPETVFFNPGNQNTEIGLPLCI
                     LNGLNRRHRWAVLEMGLAKPGDLSTLTAMFPLVCSVLLNVGSAHIGNFEDFEGLFREK
                     SEIIRTSAPGSSIVLYGNAPSIRALGSACPDRQVTYFGESLPDADGDRSVSLESYRID
                     LRGERPVTELKLKVLTRTITLSFPVFLHRGFVMDLCAALAVCSTIIDTDRLIDFSPDL
                     LTLPKDRFEIRSLRGNWMIFDHYNASYESLCTALDLLVEMKRIRFIPHFHLVLGSVLE
                     TGRHSNEIHTRIAEKILSSDPLSVYLFSRESSILETQRAMERARDGRQAASRIPITCF
                     SSSDPEAIAKGIAHDLSVSHNHIFYFKASRMVRMEEVVESVMDLLDRGNHHNSDLQIQ
                     HSSI"
     gene            49013..49957
                     /gene="mraY"
                     /locus_tag="PKM49_00215"
     CDS             49013..49957
                     /gene="mraY"
                     /locus_tag="PKM49_00215"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNY81267.1"
                     /translation="MSWIFLIEGIITILIYKYSIPLYKRWGIVQYVREEGPRLHNYKQ
                     GTPTAGGVVFLLVFIAFYTLSQWFSASSFYYPVLLTAVLFGLIGFTDDFLCVRRKNAK
                     GLPARVKLIAQLAISLIVYFFSHHGIRSTTISLVPISRTIDLGWFYPVFFVLYLTAFS
                     NSANLTDGLDGLSGGNALISALGAQIFLFLVGVKDYSMIFLAGALIGFLWFNVKPASI
                     FMGDAGSLSIGALLGIIPLLTGYSLFFVFLCLIYSLELLSVVLQVGSFKLFHRKIFLM
                     SPIHHHFELLGWKESTIVLRFWALNFLGVILALSFVII"
     gene            50036..51889
                     /locus_tag="PKM49_00220"
     CDS             50036..51889
                     /locus_tag="PKM49_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNY81268.1"
                     /translation="MILKAETLTFGFGDGRLFENVAFQIDEGDRIALLGANGSGKTSL
                     LKLIVGEYTPVEGRVVLRNGLSIGYQTQFRITDPRRTLWKECESLFSSLKETLRESTE
                     ITHDMLSFEKRIRSVLKGLGFEEPDWEREVSTFSGGEQTRISLCKLFLLDYDLLLLDE
                     PTNHLDLASVIWLQNFLKSYQGTLLMVSHDRAIVEAVANRIFEINARKIWVFNTAYDR
                     YLLQRDRMNDTLRKRKDNIVKEIERQEALIEQFRRWNREKAFKLMHAREKVVDRLKDQ
                     LKEYEGMEETSAEIGRLPVPDRTEWIVIQGKGIEKSFEEKRVLHHIDFTINRGQKIVL
                     VGKNGIGKTTFLKILAGVDPHHGGVIEKGAKTRIGYLTQTLDFRDESRDVFSELREIV
                     PAWKDYEIRKYAGRFGFIGEDVFKTISALSGGEKLKLALAKIILEKPNFIILDEPTNH
                     LDIDSIAKLQSVLSEYEGSMLVVTHDQWLIQNTFETVWLLTENAITYPRSVEDYFESV
                     RESPATFRKKTEPEKRKVEEYQAQKTLKNRLRSIETQLKEIERDYRDLECETLAIRRD
                     MNLYAHEYQKLETLLQKEKQIASDMESLIHKLDALETEHQKTLNEMEGLSC"
     gene            51883..53394
                     /gene="hutH"
                     /locus_tag="PKM49_00225"
     CDS             51883..53394
                     /gene="hutH"
                     /locus_tag="PKM49_00225"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HNY81269.1"
                     /translation="MLTIRGSGLTLSDYIRVAVEGETVTLDPSCVSLIEQSAQAVENI
                     VRKENTVYGINTGFGALASVKISPEKTKELQRNIILSHAAGVGEPAPKEVVRGMMLYR
                     ANSLAKGFSGVRLQTVEQLLKMLNAHITPIVPGQGSVGASGDLAPLAHVAMAMLGEGD
                     VWFQGKRVKAIAAMQDAGIPSLEPIQKEGLALLNGTQYIASLLSYGLFKAFRLFDWAL
                     LTASASIDALKGSTVPFDSRIQEVRNHPGQEFVAQRIRGYLRDSEIRKSHERCDKVQD
                     AYSLRAIPQVLGAVYDALLYAKGVLEREINSATDNPLVFGEEVLSGGNFHGEPVAFVG
                     DFCSIALCELGNISERRVDRMVNPLVSNLPPFLARGESGLNSGFMLWQYTAAALVSEN
                     KTLCFPASVDSIPTSAYQEDHVSMGPIAARKLLSIIENDTKIVAIELMIALRALEFLQ
                     PLRSGPALMKALEPVYALLSPYQNDRFFGDDFERIYRYISHNAAATFFETAIS"
     gene            53482..54600
                     /gene="dnaN"
                     /locus_tag="PKM49_00230"
     CDS             53482..54600
                     /gene="dnaN"
                     /locus_tag="PKM49_00230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNY81270.1"
                     /translation="MRFSVLKQNLEYVASKMAAIVSVKAIKPILFGALIEVEDGKIKV
                     SATDMETSVKMSLGESFLEGEGAFVVDAKLLEEVARNTLSDQVHFTFEENRLTYRAQN
                     SNYDLPFMEPDDFPPIHFIEEGQSLSFPRTVLIEMIEDVLYTCATDEMMKSLYCILWE
                     SEGKLLRLVGADGFRLSLSEQDIFNTEALPEFEDFRFLVSLKAMKELLNYLKEIKDDV
                     VTLVYDGKRLCLGNESHRLLVRLMDYDYPDYRRVIPKTAKTKVVLDREEFLKKLRFVY
                     VTTRTSGESVRFIFTDNELQMLARASDRGEADVEMAVQKEGNDLQIAFNPKFVIESVQ
                     HARQPKLELSFLDESSPMQINETDMPRTINIIMPMRMI"
     gene            54603..55103
                     /gene="ispF"
                     /locus_tag="PKM49_00235"
     CDS             54603..55103
                     /gene="ispF"
                     /locus_tag="PKM49_00235"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015127132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNY81271.1"
                     /translation="MRRVGLGYDIHRLATGRRLVLGGVDIPFEKGLTGHSDADVLVHA
                     IMDALLGAAGLFDIGHYFSPSDSRWAGISSLVLLEKVKGMVCGERFFRIENIDSVIVA
                     ENPRLSPYIDQMRKNLSEVLKIPCSSIMVKATTNEGLGPEGLGEGISAHAVVLLESAV
                     ASPKSR"
     gene            55123..55914
                     /locus_tag="PKM49_00240"
     CDS             55123..55914
                     /locus_tag="PKM49_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNY81272.1"
                     /translation="MARIVVGRYIPGDSILHRTHPAVKLVSLMVFVPSIFWVGAVWLQ
                     IATLLFILFGIGLSGISPIYFFRVFRFLWFFFTLIWVFQMLQAEGYILMRIGPFRLTD
                     LSVTRSIVLCMKILNGFLSSVIFSATVSPIQLSDSIESLLRLWKVKPETAQDFSMVFN
                     LSLKFFPILFDEADRLIKAQKSKGASLDYGGVPSRLKAAQRVVLPLLFNTIKRADDLS
                     IALEARGYTSGEKRVKYRRQRVGWKDWCFLTVVIGFSALMISFSG"
     gene            55922..56614
                     /locus_tag="PKM49_00245"
     CDS             55922..56614
                     /locus_tag="PKM49_00245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNY81273.1"
                     /translation="MDSFFDHQFLSETRKIVAIDEAGRGPLAGPVCSCCVFLEKEEED
                     LLLSELPFLDDSKKTSREKRKRIVRWTLDHRIPFAIGMADVEEIDCENILRATEKSME
                     RAVAKLGLPFSLALVDGAHLRLNFPHRLVPKGDAVSVRIALASNLAKHYRDSLMESYD
                     TQYPRYGFAEHKGYGTLKHRKAIREYGASPFHRLTFAPLCRETDLATLSAWVAGGEIS
                     SHRYEIILKKRG"
     gene            56621..57922
                     /locus_tag="PKM49_00250"
     CDS             56621..57922
                     /locus_tag="PKM49_00250"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HNY81274.1"
                     /translation="MRMVDLIQKKREGGELSQNEIEYLIGEFVRGHIPDYQMAAFLMA
                     VYFQHMTEAETFFLTRAMEYSGQVMDLSTIKGIKVDKHSTGGVGDKTTVILAPIVAAC
                     GVKVAKLSGRGLGHTGGTIDKLEAIPGFCTSLDFDQWVKQVNEIGIAVAGGNEQLVPA
                     DKKIYALRDVTATVEEVSLIASSIMSKKLASGADAFVLDVKVGSGAFMKTLPEARTLA
                     ALMVKIAARSGKKACAFLTNMDMPLGKSVGNALEIIECVETLKGEGPRDLTDICLSLA
                     IKMVEYGAAISEQEAKNRVLERFENLDALNKFAEFVRAQGGDTAFIEEPGLLLADVKV
                     LPWFARADGWIGRMETEEIGKAAMVLGAGRETKESVIDPAVGLVLEKKTGDAVERNEI
                     LGYLYYREEKRAEEALEILNRAIDYDTEPVEAPRLILDIVA"
     gene            57975..>59413
                     /locus_tag="PKM49_00255"
     CDS             57975..>59413
                     /locus_tag="PKM49_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81275.1"
                     /translation="MRYLFVLLLVCVGCIGPAFSQELFYVKQHGDYVRIKEPDVIEMS
                     GKLLISVTALRLFGAGVTNFGESSKYYIVDPIEQKTLIADPLKGITLDFSEKVDGFVI
                     VINRMTYINADLAFHFLGFEFEHEERYYVYKPQHRLTLREARIDGRTVKFEFAESFFN
                     EQDVIKVQTKGSAAEITLFPVDASQGIIQDLTLKESDTFFSLFQVEFNVPVAYRIDRA
                     GSSVAVYFSHVDPYLEEIETVANGIQWYRKKETLAGYTLRVSYLEILLKVAEIEISPE
                     LALGGIGYKEKVSAMAKRNTAYAGINGCYFDTVTNFPIGILIKDGKLLSEPYYYPRPY
                     FVQTSDGKYAILNLDQEIQLSLGASLFLVKGVNKFSKNGDVIIYTEEFSQQIPRQGDR
                     EYVVVENNRVISKEYAIRAPRNGYVVMLSPIGVSKYVKIGDSAEIRTIVSGYPYEIQF
                     AIEGGPQILEGGKLISSVDTEKVRYGERL"
BASE COUNT        15155 a        15115 c        15237 g        13906 t
ORIGIN      
        1 atctttaaga catgccaact taaaagttgg cgttcccaag cgtatcgcgc cgtcgcgaaa
       61 agaagaagcg cttacgccgc gatgctgcgc tcgagaactc tcccagagat aagataaaac
      121 caaaccaaac aaaagttcgg cgttcacatt ttggtgtacc ccgatataat acccacacgg
      181 ggcattccat tcgcttcccc ctatggtata attgtaccaa ataaatgaga gggagtaagg
      241 agtgatgaaa tgcgaatggg taaagttatg tcctgatcag gctatcatcg accggatttc
      301 ccagcggcac gggatatcgg gcatattggc ccgaattttg gcaaacaacg gcatcgtcga
      361 agacgtagag atagaagaat acctctatcc cgagcggacc ggtttttatg atccctttct
      421 gatgcaggat gtggataagg ttgtcgacta ccttatcaaa attcgtcaaa agaaagaacc
      481 gatcgtgata tacggagact atgacgtgga cggcgtgacg tccaccgctt tggtttactc
      541 catccttcac caactggggt ggaaggtcaa ctactacata ccgtcgcgct tagaagaggg
      601 gtatgggctg agtcaagccg ctatcatcga tttattcaac aagaaaaacc gcaatatcat
      661 caccgtcgac tgcggaattt cctcttttaa ggaagtagac tacgcgaact ttctcaactt
      721 caaggtcatc atcaccgatc atcatgaagt ccaggagaag ctcccgaacg cgttggcggt
      781 tgtgaacccc aaacgaaggg acgaccgcta tccggataaa gggttggccg gtgtcggcgt
      841 cgcgttcaaa gtgatggatg ccttactgcg ccgcttgggc aatccgatcg acattatgga
      901 atacctcgac ctggtcactc tgggtaccgt cgcagatatc gtgccactga aaggcgaaaa
      961 ccgcgtgatc gtccgaaagg gaatgcggct catagcggaa acgaagcgca tcggcctgaa
     1021 aaagctgatg gaaatatccg gcatcaacgt cgagcaaatt atgactcagg atatagggtt
     1081 caaaatcgcg ccccgtttga atgccgtcgg ccgtttggaa agcgcctatg ccgcgttaaa
     1141 actgcttcta tcccaagacg aggccgaagg gatggagatc gctcggtacc tctcctcgca
     1201 aaatgagaaa cgccaattca tagaaaacca aatctatcaa gacgcggaaa cgatgctaaa
     1261 aaacgatcgc gatctgcagg aggcgccggt gattgtctta tggaaagaag actggcaccc
     1321 cggtgtgata ggaatcgtct cttcacgtct ttccgctaag ttttacaaac cgaccctgtt
     1381 gctgtccttg gattacaagg acggtgtcgc cagagggtcg ggaaggagca tcgaacaggt
     1441 caacattatg gatttgttgt ctcgggccaa cgtgaaaggt ttggagtacg gaggccaccc
     1501 tatggcggcc ggattcactc tccccgcaca ggaattaacg gttttccgcg agcaaatctt
     1561 cgaagcttac cgtgcgttgt acaaggaaga ggtgttcact tccaagatct acttggacgc
     1621 cgagctgccg attgacgaga tcaatgaatc tcttctcgac tccctcgaaa tcctgaagcc
     1681 tttcggccaa tgcaaccacg aaccggcgtt tttaatcaaa aacgccagtg tggaccggtt
     1741 gaaaatcctc ggaaaccatt ctcaacacat ccgcttgatc ttgaaacaag gaagccgctt
     1801 tttggattgt atcgggttca atttgtcaga acggctagaa gagttcagat acatacgccc
     1861 gaatttgcta aaagccgact gcgtaggcaa tttgaaggcc ttctggcatt tcgggtcgaa
     1921 acagatacag ttttcaataa aagatatcca tttctacctc gatcctgcag taaaagaagt
     1981 agaacggacg accaaagcgc tgtttagcat ttcgaaaaaa aacgaaccca catctgcgga
     2041 gcctggcagc gaaaacctgg aaacccttca cgcggatatg cggcaacgtc tgttctccaa
     2101 gtaccctttg ctgcaaaaag ccttttccga tcagagccga tacgccattt tcgccagtcc
     2161 gaaaacacag gatgcggtgc tttcgcttaa gtgtttaaac gcaatctcga agggacagag
     2221 gctgttgctg gtaacgccca gcgcctcttt ttccgtcttt cgttcacagg tttttaaaca
     2281 gttcgacatc tttcctacgg tctcaaacgt cgttttagag ctttcgtctt catcgagcgc
     2341 ctgcacgatc gtgacgattc cttatctgat cgcccactac gaggagctaa aacgggactt
     2401 ccacgagttt gtttttttgg atatcgagta ccttttccac gaaaaaatgg aggcgcggcc
     2461 cgttcttagg caattggcag acctgctgcg aacgaagact cctccgttta cctacgtggg
     2521 atcctttctc ctacccgaaa aaaaagccgc cttaaccacc tttttcccgt tgagcgggct
     2581 cttgaccgaa ccgatgaaga gcaccagaat ccggctcgtc gatcgccgcg gcatcgctga
     2641 cccccttagt tatattcagc agcaggtgaa aaacggggag ttcgtttgcg tgtccgttca
     2701 aaaaccggaa cgcaccgtcg gattgaccaa agagttcgga aagatgcttt ccgagcattt
     2761 ccataacgga gaagtcgtct tctacaacca acagctgaaa ccgttccaaa agtccaagat
     2821 cgaggacctc gttatggaga acaaagcgcg tctgctgatt actaccccag atttcagcag
     2881 ccatctcaaa tttcccgcaa atgcgaacct ttgtattcta gaccccctaa agaatccctt
     2941 ggaagtcctc acggtgtcga acgccgtcgg aacgcaaaag tcgaacgcca tcctccacct
     3001 gctctacgat accaagccga cccagggcca ttcggcggac cgaaaacata gactggaaaa
     3061 aatgatcctt tctgtgctaa aattagccag ctcagaacat agggcccctt gggaaaaagt
     3121 acgcgacctt ttcgtttcga aaggagaaac tacccggttc gaagccttta gcttacggac
     3181 cttcgtggaa gaaatcgcac cggcgggtaa atatgataac ctttcggaac gagaaaaagg
     3241 gctgtttaaa tcgttgcaga cgcaccctga ctttatagaa tttaagtggg aatggtttat
     3301 taccgatatc tatgaaagag ccttttatag ctatgatgca aggaaaatcg tggaattgtt
     3361 cgacctaccc tatttaccgt tggtttaacg cggaggtgtc ttcttgcccg tgtacacgca
     3421 atgcaataca gacagtgaac tgaacgcgtt attcattttt tgtgatatcc aagagattac
     3481 ggaggccttt gaggccatca agaaaatgag atttttcgct aagattgtcc ccacgcccaa
     3541 ggatgttttc ccggcaaaat ctttgagtat cgcggtaaaa aaggaagatt tgggcattct
     3601 cagcgataag ctccaagaac taaagatcgt accgattaag tccgtgactt ttgaaaagtg
     3661 cgttttttcc tatccctaag gctactattt gaaaaatgat ccttgcctgt gattacggca
     3721 agaaacggat cgggatagcc ttcgccgata cagaatcggg cgttatcgtt cgatctggcg
     3781 ttctcgaaca aacgaagagc gcggtcgagc gtataaggcg tatcgttcag gagaaaagcg
     3841 tccagaaaat catcgtcgga ctcccgttga acttgagatg ctccttcacc gagtctaccg
     3901 acgccgcggt gagttttgcc tcttctttgt ttgatcgggt tagtcgggcg gtgtttttca
     3961 tcgacgaacg gtttaccacc gcctccgtct atgtaaccgg caggctgttg gggaaaagcg
     4021 aagccgagag tcgcatcgcg gtggatgccg gaagcgccat aagccttttg gacacgtatc
     4081 tgaaaaatcc aaaaaccgcc ttgcccttct ctcggaaaac cttgtcacca gaggacgttc
     4141 gaagaatgat cgaagagcga acttcccagg gcagctttgg agacaagccg atagaactgg
     4201 taatcaatgg gctcagccgg ttctcacctt tttcggatac ccagcgattg tgggccttga
     4261 gagaacaaaa tccttggcac ttttccaagt atcgggcaat cgaaaaaccg gaagcgattg
     4321 tagagtattc ctttcattat ggacattagg tgcccgtagc tcaattggat agagtattag
     4381 actccggatc taaaggttgt gggttcaaat cccaccgggc acgccatcat ttggaggtga
     4441 cggtatgatc ctaatagaga gcgcctttaa ggatgaactg gaaccgattg ttcaacaact
     4501 cgtcgtattt gaggaaggaa aggtcttcga tagagactat acgcgagggg tcatcgggcg
     4561 aaacgaaatc gtcgcatgtt cagggatgac gggcaaggtg gaaaccagtg tgatgactca
     4621 aaaactgatc gaactcttcc accccgcgtg cgtctttttc gtttcttgcg ccgttggcct
     4681 cgctccggac ctcaaccctg gagacatcgt cctaggcgag gcctaccgag aacacgaccg
     4741 tccgtctttg gacgacaccc ttcttccgtt aatcaccggg aatatcgagt tcgcgtaccg
     4801 gttgcgccaa gagtatccgc acttaaaatc cggagtgatc atcagcggcg acacgattcc
     4861 gacagacgct cagaaccggc gcgccctata tcttacccac cgggcttcag ccgccgatat
     4921 gcatagcgca gctgccgcta tcgtttgcag gatgaacgga atcgggtttt tatctttcca
     4981 gacgattgta ggtcggaccg atttcccctc tctcgaagcc tacgaccgcc agcgtagaac
     5041 gcatgctgcc gctgccgcca cgtttttggc ttcctacata gaaaagaact ttttaagcat
     5101 ctcctgcgcg gaaaaaaaat agcggtcttt actccgccga gccctatagt ccagttactt
     5161 cacttaggag acgataccgt ggatcataca ggcctagaat cagagatcga ctctcttcga
     5221 ccccagattc ggtccgtttt ttcggagttg catcgggtga tcgtcgggca ggaagagatg
     5281 ttggaaggga taatgatcgc gctgatgacc aagggaaacc tcctcatcga agggttgccc
     5341 ggtttggcaa aatcgctggc cgtcagcacc tacgcaaaga tctccgagct ttcttttcac
     5401 cggattcaat tcaccccgga tctactcccc ggagatatca ccgggactct gatcttcaat
     5461 atgagaaacg agactttcgt cgtcgagaag ggtccgattt tcgcgcactt catcctcgct
     5521 gacgaaatca accgggcgcc ggccaaggtc caatcggctt tattggaggc gatgcaagag
     5581 aaacaggtgt cgataggcaa gacaacctat ccgttaccca agccctttct cgtcatcgcc
     5641 acacaaaacc ctatagaaca agaaggaacc tatccgctcc cagaagcgca ggtggaccgg
     5701 tttcttctga aactcgagat caattatcct accttcaatg aagaaaaagc tattctcgaa
     5761 caaaaaggag tggaccaaga cataccgcta aacccttgcc tgtccctccc attgatagaa
     5821 acgctccaaa gggtcaccga tgaagtctac gttgacgaaa aactcgtgac ctatatcgcc
     5881 aagatcattt acgcgacgcg agaaccgacg cggtataaac tagacgagct catccccttc
     5941 attcggtatg gagcctcacc cagagggagc ctgagtttgc tgaaagcatc caaagcctac
     6001 gccgtcatcc aaggacgggc ctttgtcacc gctgacgata tcaaacgaat cgcttatccc
     6061 gccctgcgcc accggatcct attgaccttc gaagcagaag cggacggact tcaaaccgaa
     6121 gagatcatca gccggatcct ccaaggactc ccggttccat gaaaagacag gatcccgagc
     6181 cctctgcgga gttcttggag aagagaaagg agatactcag aaggatccgc ctgttgcggt
     6241 tgttcgcgga aaggtccatc agttctcaaa ctctcggtat ctaccgctcc ttttttcccg
     6301 gcaccgggat ggacttctcg gagattcgag gatattattt cggagacgac atacgcaaca
     6361 tctcttggcc caccaccgcg aagaaccgga aactctacgt caaatccttc aaggaagacc
     6421 ggtcgcttaa cgttgtaatc gcggtcgact gttcccgttc gatgggattc tggagtaaaa
     6481 cgcggcggca ggagaatatg gcttatatcg ccgcctgttt ggcgttctct gcgcttaaag
     6541 cgggagataa agccggctta gtgctttttt ctgatcgcat cgtcgattac ctcccggcga
     6601 agaatgcgat gagaaacggc gtcgtgatca tagatgcggt tctacgcgcg aacccgtcgg
     6661 gaatcgccga tttttctgtt ttacatggat tcctcttgtc gattttgaaa gaaccttcgg
     6721 cgatcttcct gatctcggac tttttcctct ctgaacccga aattgatgac ctcgtcgcga
     6781 agttgcgcgt gctgcgcgtt cggcaccgcg tctatccttt cagcttttgg gaccaagccg
     6841 agttcgttct cccggagctc ggatacgccc gggtgcggga tatggaaaca caacggaccg
     6901 aatggtgtaa gatcagcgaa ccgttaatcg aagactacca tcgtcagatg gagaaaaagc
     6961 gcacctatct gcgggagtct ttcgaaaaag cagggatttt ataccttcca ttactggaag
     7021 gagaggacta tggtaaacgc cttttcgatt tttttaagaa gaaaccaccc ttataaaggg
     7081 acctctcgaa taggggtatc tatcgctctt ttcgcccttt ttctgctcgg tttgggagga
     7141 atgccatacg ctgtggccgc ggccggcggc cctccgacct accctgtaat agggacacgg
     7201 gttcgaacgt tcgccgccta ctgcgaccag acggaagtga ccttgggcga aacgtttcgg
     7261 gtcgtggtca cggctcgtta ctttccccac gagctggtaa catgcggagg gctggaagcc
     7321 cttcacgaat cgcttgccct caccggggtc tctacagtct cacacttgtt ggcggttaat
     7381 tacgtggaag aaacgtggat ctacaccctt acccctacga cggcaggagc ttttcttttc
     7441 ccaccgctat acgcctcttt ctgggagcct ggaacgcccg cgaaccaagc ggtgaagctt
     7501 tcgagccgtc ccctctattt ggacgtctac gccctcgcgg ctccatacgg ctttcctgaa
     7561 ggggagctga aaaaagtggc gcctatttcg ccttgggcgc gttggatcgg ttggctgatc
     7621 tccttgggac ttctgatccc ttgcggaacg attctccgga aggcgttcgt tcgccccaag
     7681 cccgcaaaag cgcaaccgga gaagcccgaa ggtttggagg cgctcgagtt attagaagaa
     7741 ctgaagaaag agtattatct cttgttgaag gattccaaaa cgatcaacac ccccgaagac
     7801 gtgaaagagt tctactatcg actctcgcac ttcgttcgag ggtatttcga cgccgcttat
     7861 cagactcaga tgtcgatgaa aacgccctta gagatacgat ccgttttttc aggtacgaaa
     7921 gagatcgaaa tgaagacgct agaagaggtg ctcagcttct tggttcggtg cgactcggtc
     7981 aaatacaggc cgctcgcccc gctatcggag cctatcgact ccgacatccg atggctgaag
     8041 aaaataaccg cttccatctc taaaaggaag atcgggtaat cggaggaaca tgttccgttt
     8101 tttgataccc acggcttttc tgttttttat catcgttccg atcgcgttgt gggccgctta
     8161 taagagaaac aagcggaaag tcttctcttt ttctaacgcc gatatgctct tcgcacttaa
     8221 aaaaccgaag gccactgtcg agaagtgttc ttggtttcta aaaatcgccg ctttgatcct
     8281 gttcgtgttc gcttgcgccc gtcccgtcca aacgagggat gtgtcgctgt tcacgcaaca
     8341 ggcctcttta gaccttctgt ttaccatcga tatctcttcg tcgatgaatg aaaccgccac
     8401 ggccaacaca agtgataaat cggggctggc gcggatcgaa tacgcaaaac aagccttgta
     8461 cgccttactg gacatcaccg agagagagcg cgcggggttg gtcgtcttcg cccagaatcc
     8521 gtatacggtt ttacctctca gtttcgacca cacgccttta cggtactacg cctctcgttt
     8581 acaggactat ccgggcacca tcgaagacgg aacggccgtt tgggattcgc tgatggtctc
     8641 cgccgaacgc ttgggaaccg tggattctcc cgccaaagtc atcatactca tcaccgatgg
     8701 cgccaacaat tgcggttttt ttacggaaga ccaggtttta gaacggttgt tgcgcgatga
     8761 tatacggctt tacgtcgtgc atgtcgggaa ctacttctct tttacgctca cgacgagagg
     8821 cgaaatcgac aagattcgca aggtatccga acgctcttcg ggggtgtttt tcaccttttc
     8881 cgaagagagg aacatccaaa agatcgcgaa acagatcgaa tcgctggagc gctcgataga
     8941 agaaaaacag gagcgtctgc gctatcatca agactactac ctttacctgc tcgtccccgc
     9001 actggtctgt ctttctgttt ccttaaacct cgattggatt tacaggaaag ggtatctcct
     9061 atgaagttcg ccaacccctc ctacttttta cttctgttcg gactcatccc ctacgcgttt
     9121 tttttattcc tgcggtatcg taaagccgag aaacgcaggc gggcggattt cagcgcggag
     9181 gccatcaaag agctttatcc ttcccgttct atcgcggcgt ttttcgttcg aaaccttttc
     9241 atgctggcgg cgttgctctt cgccttgatc gccctcacca acccatacac gcgggagtcc
     9301 ctgatatccg cgccccgccc cgtcaaagac gtgatgatcg ctctggatgt cagtctctct
     9361 atgaatgcgc gcgacgtgtt tccctctcgg ttcgaggcgg cgagacgaga gttgttgcag
     9421 gtgttgaagg tccctaatct tcgcgcgagt ttgctgttgt tctccggaaa aactctgttg
     9481 accttgccat tcacccaaga caggcaagcg ttgtcgctat tcgctcaaga actgcagccc
     9541 ggatatatcc gttcggaagg caccaactac caagcgctgt tcgaaaccat aatcgaagaa
     9601 tacgatcatt ctctctcttt gtcgaaagag ttaggctgga tcacctcgag agcccccaca
     9661 cacgtcattg tgttaaccga cggagacggc atcgaatggc ctgaagagcc cttattttac
     9721 gagatgcgca aacgggatat cattctgtgg gtcgaagcct tcggttcggt ggagggcgca
     9781 gctatccctc tctataacga aaacggagag ttcacggact acttaacgct gaatggcgta
     9841 cgttatcact caaagaacca ggaagccttg ctcagaaact gggtagagtc aacgcgggga
     9901 tactacgtct cgtattctac gacagaaagg ctttcagaaa aagttttcca aatccaggac
     9961 aagatgggaa ccgacgcagt ttttcaagac tatttggcga taaagaatac cgtattccct
    10021 gcttttctcg cgctggcgat cctctcttta ctgatcggaa tggtcttatg agacggatgg
    10081 accgtagatt gaaaggattt ctcttcgctc tgatcggtat gctgtcggtc gccctccttc
    10141 tgtcttcgcg cccgatcccg ctcgacccaa cccttcctag aagcgtgctc aaggcggcgg
    10201 aaaaccctcg agatatcgtg agcaacaacg atgcggcagc tgattattac ctcttgggag
    10261 agcggaacct cgccagggtc tatatcgaac gggccctcca ggaaggaaac cccgacgatg
    10321 gccatcggga tgagctgtta acgaactatg gcgatatttt gtaccagctg gggtatttgg
    10381 aggagtcttt agaagcctac caaaccgcgc tctatctgaa tcccatcaac gaacgcgctt
    10441 ctacccatta cgagatcgcg tataatcgtt acctaaggca cctagagact caaatctccg
    10501 aagaaggcgg gatagaatca gaaaggacgg aggaaagaga aggggaagaa agcggggacg
    10561 ctgaaggtga gaggggccaa gaaagcgatc agaacaagaa agagagcgaa aaaggctcta
    10621 aacctcaaga agaaaccggc caatcgagct ctgacgatcc ggaagagaac ccggaacaga
    10681 aagaacacaa gggaaaaaga caggacaagc aagacaaagc cgaagacgaa aacgtttccg
    10741 gtacgggaca gcaggcagag gggtcgacaa accccgaaag cgatcagcgc gagcatagcg
    10801 tgggcttcgc cactttggaa gactactatc ggaagataaa ccaggacgat acccggcaaa
    10861 tttacaagtt cctttttccg gacgaaggtg accatggaga aaaaccgcgt ttgccctagg
    10921 ctcaaaggca tcttggtttt catagtctta tacgcgctct ctttcgtttt ggggttcgca
    10981 ctgcaactga gtatttcggt tgacccccct tttcagcaga tagaaagagg aacgcctttc
    11041 ctgatccgct acgtgatttt tgactacgaa ggggaagaaa agaacctgac ggtctctttg
    11101 gatacccaag ccgccttttc cggaaacttt gacgaattct tagcttccgt tatcgatcat
    11161 gcaatcaagc ccttccaaga actggggagc atagaccgag agctcgaaga tggggccgtc
    11221 tccggcggta tcatatgttc gagcgcgctc gtcggcacgc acgaattgga agtactcgtc
    11281 ttcgagaagg atcgcttggt caagtccgaa tccttagtga tcacggtcac cgaaaatcat
    11341 cgcctaagcc aggccttaaa cgcgcgttgg gaaaaaaaga agaaaccgga gcactttggc
    11401 ctgtcgttcc tctcggaaaa cgcggttttc tataacgaac agtactcctt ttcgatgatc
    11461 gcggtggaga aggtcagcgc gtcggagact ctaaaacttt ccggcttcta ttactacact
    11521 cccgcgctct ctcaggccaa agccttgcgg tacgcggcgt acctcaccga aacgctcggc
    11581 ggggagacct acgcgctcaa gatggcggac gtcgtcgtga accccaaccc tctattggtg
    11641 gaacaggcga aagtgaaggt ttatcacccg tacctgcgat ggatagacgc ttctggcaaa
    11701 gagatagaag acctctctta cgaggacgaa tacgaaaccg tcctctatca gaaacccacc
    11761 gactcctcct ttttccaagc cgatcccatc gttctcaaag agtgggaagc ctcccccgcc
    11821 ctacagacgg gagaaatcgg aaccgttcga ttttctatcg aaagccgaag cctctttttt
    11881 gggtctactt tcaagatgat ctcaatgcca agcgcgcaga cctccctcac cttacgccgt
    11941 tcttggatca ccgaatccga aggaatcagg agaatcgaca ggaccaccca ttacgaatac
    12001 gattttttac cggaatcagc ggcgacgtat acgctcacga tcgaaccgtt cgcctatacg
    12061 gtaacgagaa cgggagaaac gcgggttttt caaacggacc cgctaacgat cgtggtcaca
    12121 caaaggctag aggataagcc gttcaccggg atcctcaacc cattgaagcc gctgagagaa
    12181 aattcagctc gcgtgaagaa taagcaagaa ttcgaaaaag gcctcctcta cgctctcgaa
    12241 gaaaactggg aagaagccac gcgcgtcttc tcgcgattag ccgaagaaga gccgctttcc
    12301 gacgacattt ggtacaatct ggggtgggtc gccttttcca gcgatcaggt cgggctctct
    12361 caactatgtt tttatcgcgc cttcacgatc gtggaaacgg aggacactca atacaacatc
    12421 cgtttcatag aaacggccaa ggggttgagc gggcgaccgc tcgcgaaagt gaagaggatc
    12481 gatgggctcg cccatcgctg gctaggcgtc ggaggcgtcg tctgcttggc tctattctct
    12541 ttctcgataa ccctacgtct taggcgtcta tccgttttta agaggaggag agaagccgag
    12601 cacccggaaa aacacagacg cctgtcccgt gctcttctgg ccttcattgc cctgatctgt
    12661 ctcgcatttt gcgccgcgaa cctcctacgg gaatacgcct tcgagcggca agccgtggtt
    12721 ctatttgaaa gccccgctta cggcggaccc gctttcgact atccggccat cgggacgatc
    12781 gccccaggga acgtagggaa atgtctagcg cagcgcgacg ggttttccta tatggaatac
    12841 ctcgcgccct gggcgatcga cgcgcctttt tcgggggacg tcacctctgc agtccgttat
    12901 tgggttcctt cagagaactt ggaggtgatc ctgagtactt tgttcacaga atagccaagt
    12961 gaaaaaccat gcgcttgagc ctatgttgaa cccatcgtcg gataatcagt ataatccaaa
    13021 tatagtatta tggttacagg aataaggtag tcaatcatga taaaatatcg ttagaatatt
    13081 ctcgatcttt cgatactgag gtgagcagaa tggcccgcca taataaaaca gacagacagc
    13141 aagaaccgca accgtcaaaa agaggattac gcgatttttt gaatgaaaac aacaaatgga
    13201 ttcgtttaat ggacaagata ccctggagcg aactggaagc gcggtatacg aagactttta
    13261 tctccgagaa gacaggggga agccggcgat caacgcgcgg attgccttgg gggcttttat
    13321 cgtccagaca ctgctcaaga cgacagacga agaaacggtg gagcatattg aggaaattcc
    13381 gtatattcaa tgctttctgg gactgggagc gttcacgacg gagcccatca tcgcgagcag
    13441 ttcgttgtcc cagatacgcg aacgcttaag cgaagaagac cgacaggcga tcaatgagat
    13501 gttgatggac gcgattaaag gagaagagaa ccactggaaa accgagaaag aagaggaact
    13561 ggtggtagaa gaatcgatca agagggaaat ggatcgtatg cgcgaagtga ttaccgagag
    13621 atttcgccta tggaggtatg gccccgcaga ggattagcaa attcccgaat gttcatatac
    13681 ttgatcctgt tttgttacct gaaaggagaa ttcgattcgc aaaagcggtt gtgatataat
    13741 accccgatta tgcttgaaga aggttataaa gccgaaaaaa ggtaccttcc cagcaactga
    13801 aaaaaaggag ggaagacgat gtatgccatt attcatgcga gcggcaagca gtaccgtgtg
    13861 gaagaggggc aagttttgta caccgaaaaa cagcctttcg aaaaagacga acgtatcgtg
    13921 ttcgaccgtg tgctgatggt caaagacgag ggagtcacga taggaaagcc ctatatcgaa
    13981 ggggcgaaag tgatcggcac cgtacaagaa cacgttcgcg agccaaaagt gctaattatc
    14041 cgctttaaag gtcgaaaaca gtatagacgc acacgcgggc acaagcaatg gaaaacggcc
    14101 atcctcatcg acaagataga aagggcctga gcgcaagagg aggctcgtga tctgttgcac
    14161 ggtttttcga accgcgacgg gctatcgagg atttgaggta accggtcacg cggggatggc
    14221 gcgaaaaggg aaagatgtgt tgtgcgccgc cgtgagtatc ttgacgacac acacggcgat
    14281 gacgattcaa gaagagttgg gaaagaaagg aacgggtaag cagacggcag ggtatttcag
    14341 attcgagatc acggacacag acgagactac ggatatgcta ttcagagcat ttttgaaacg
    14401 gatgatcggc ctatcggaag aatacccgcg gaaaattaag gtcgaggtga aaacatgaaa
    14461 ttgcatctag atatacagct gttcgggcat agaaagagcg gtggcggaaa gaacgggaga
    14521 gacggcaatc cgaagtatct gggcgttaag aaaggagacg gatccaaggt cacccccggc
    14581 aacatcatcc tcagacagcg cggcaccaag atttacccag ggttaaacgt tgggatcggc
    14641 agggatttca cgctttttgc cctcgccgaa ggcgtcgtgc ggtttaagga caagcacaaa
    14701 cgtaaatacg ctaacgtcat cgtcgatacc cccgcagaca ctgccgcaac ggccgtatcg
    14761 gaatcgtaaa accgaaagaa agcgcacgtc gaaggaacat ttcgcggatc gattgatcca
    14821 gcggatctgg caagaggagg aaagatgatt tacgaacaaa agagttttcg tttaaagcag
    14881 gaagagatta cgcatccgtg gtacgtcatc gatgcggaca atcaaatcct gggcaggtta
    14941 gcgacacgcg tttccatttt gttaaccgga aagaacgacc cgcgttatac ccccggagtc
    15001 gattcgggag cctttgtggt catcgtgaac gcggaaaaga ttcaaatgac cggtcagaaa
    15061 tgggatcaga agatatacgt ccgacactcc aagtatccgg gaaatacgaa ggaattggcg
    15121 gcgaaagatg cgctgaagaa aaaacccggc ttcatcatcg aaaaggccgt gaaacggatg
    15181 ttgccgaaaa accggacggg attaaaaatg attaagcggc tcaaggtgta cgctggggcg
    15241 gaacacccgc atcaggccca gaaacctgtc gagatcacgg tataaggagg ataaaagata
    15301 cctattatgg agaatccaga ctactacggc accggtagaa gaaagacctc cgtcgcgaga
    15361 gtccacttac ggcccggaac aggaaaaatc aaactcaacg gaaaacccct caccgcctta
    15421 aaggactacc tcttgagaga ctccttagtg atcgaggcga tgaaaccgct caccttgacg
    15481 ggattggccg atcgattcga tctcgtgctg aaactgtcgg gcgggggact ttcgggtcag
    15541 gcgggggcgc tgaagatggg aatcgccaga gcccttctcg aatataataa cgacctgagg
    15601 ccgactctga aagcggcgat gctactccag cgagattccc gaatggtcga acggaagaaa
    15661 tacgggttga aaaaagcgag aagagcgcct cagtactcta agcgttagaa taaactcgtg
    15721 cttgggcgcg tcttagtagt actcaaatgc gagtgacagc atccaataga agagggaaga
    15781 gcgttcttcc cttcttttgt gtggaaaaga acgggaggcc cctcttcctt attaggagtt
    15841 ctcgatttgg agcttaaaga gattgtgcga gaagtcaaag acgcggtcag catagtcgat
    15901 ctgatctctt cctatcaaat ccagatccag cggtcaggga agtactacaa agcgctctgc
    15961 ccttttcacg gagaaaaaac tccgtccttt ttcatttacc ctgaaaaaaa acgttaccac
    16021 tgtttcggct gtggcgccac aggcgatgcg atcaagttcg ttcaggaata tgagaagatc
    16081 acgttcatag acgcgctgaa ggagctggca tcccgctacg gcgtctccat acagtcccta
    16141 tcggacagaa ccaacccctt gaagcagctc tacgtcgata ccttcgaaac cttggccaaa
    16201 cagtaccatc tctccttact ccatcatccg aacgccttga aatacctgga agatcgcgga
    16261 tacgggaaaa gcgaggcgca acagtttcag atcggatact gcgatcttcg gtttccgatg
    16321 aagtcctttc agcaatggct gaaaggaatc ggaagcgagc ccgaaaacga acagagatgg
    16381 aaagaatggg ggctcatcaa tcaggacggg tcgagccgtt tctacagacg tatcatcttt
    16441 ccgatccgcg accgttacgg cgcttgtatc ggattttctg gtaggctgtt ggagcgggct
    16501 gaagaccagt tggatcccac ggaaaaaaaa gatagtggtt tcgatgcgcc taagtatctg
    16561 aactccccac agacaccgtt tttcaataaa agtaaagttt tgtatttgga gcatgtggcg
    16621 cgaaaaccga tccaggagtt cggattcgcc atcgtcgtcg aggggttttt cgacgcaata
    16681 cggctacacg ccaatggtat ctctaacacc gtcgcagcga tgggcaccgc tttcggcgcc
    16741 gagcacttcc gcgttttaga caggttgacc aaacagtacc tgtttatctt cgacagcgac
    16801 gaggcgggta aaaaggccgc agagcgggcg tttttctcct taccgtcgga ggtccgcgcc
    16861 atgggatgct ttttgagcga ggcgaaagat ccggacgagt accttcgacg tatcggaaaa
    16921 gatcgcttca tccaagagat ggctgcggcg aaacctctcg aagaattctt gatcgatttt
    16981 ttaaaagatc ggttcgagat cacccaatcc gtaggccaag acgaatacct cagggcttcg
    17041 ctacccatcc tgacaaaagt gaaagagatc ggtaatctcc cttcctatga aaaggtcgtc
    17101 ggtatcgtcg ccgaaacgtt aggaatagag cgtccgaagg tggagtcctg gtatcgagaa
    17161 catataacca aaacggcgac cacgaaacat acgccggaga aaaccggctc cgttcctatc
    17221 gaaccgaggg aaaagacggg caccgagaaa accgaaacgc gggccctctc tacgggaaat
    17281 cgcctttcga ggtgggcagg cgaagagaac ccagccgaag agcatattct ggctatctat
    17341 ctagaaaggt cttcgtttcg caaagagatc gagaccttcg tccaggaaca cacggcatac
    17401 ctcaacgacg acttctgctc gttttttgac aaagccagga aggaattcga accggacgat
    17461 gccaccaacc acctcaacga gtttttccct aaagaatttt tacatcgcgt ttttcgtttt
    17521 atgaatcggc cgttctttat aaaggatgat cccgcgcgga tttctaaagc gctcggagat
    17581 tgtttcggga tcattcgcgc gcggcgggag gtttcgcaaa tgaagaacct ggagcggcag
    17641 atacgagaca caacggacct ggaagaaaag aagcgcttaa tgaaacaaag cgtcgaattg
    17701 atcggcgcat cgaagaaaaa aagcaaaatt gaagggaggc ctttctgatg gaggagaaat
    17761 ccgaggtagg taagaacctt cgaaaagaag aaacgccgac acccaaacgg agcgccaaaa
    17821 aaccgagcgc atccaagtct ggcggcctta cgaaatcgaa atcggggacg tctaccacga
    17881 aaaagaaaaa aaccgtcgcg ctggacctcc agtcaataaa gcttccggaa tgcttaacag
    17941 aggctaactt acgaggcgat gccgtagaca tcaaaaagag tatgacggaa ttggccacga
    18001 ttggttcaaa aaaagggtac atcacctacg aggatatcga cgcctatttg cccgcagaga
    18061 tggaaggatt cgacagtaat tttcttgaga tggtctacga gacgctcgag aaaaacaaga
    18121 tcaagatcat agagaaagag ccggaggtgg acgaatcggg gtccgaggaa ccggtagagg
    18181 atgagcttgt cgagcggatg atcagcgaag acgggcctgc catcttcgac aacaacagtt
    18241 tcaacaaaga cggggttaag atgtacctca aagagatcgg ccggattccc ctcctgacgc
    18301 ccacccaaga aagaaccctc gcgaggaagg cgcaaatagg agacgaacgg gcgaaacaaa
    18361 aattggtcgt cgcgaacctt cgactcgtgg tgagtattgc caaacgctat atcggaaggg
    18421 gcctttcttt cttggacctc atccaagaag ggaacatcgg gctgctcaaa gcggtcgaga
    18481 aattcgattg gaaaaaaggg tataagttca gcacctatgc cacctggtgg attcggcaag
    18541 ccattacgcg agcgatagcc gatcaggcgc ggacgatccg cgttccggtg catatggtgg
    18601 aaacgatcaa caagatcaac aagatacgga gagaatacct tcaagaatac gggaactacc
    18661 ccacgacgaa cgagctggct accttgacgg gtcggagtat ggagaagatc gaggagatcc
    18721 tccaggccga caaagagacc gtctccttgg aatctcccgt cggcggagac gaggactcct
    18781 cggtcgggga tttcgtgccc gacaacgaaa tgatcaaccc ggaagagtcc gcgatgctga
    18841 tgctgttacg cgacgagatc accgaagtac tcaacacatt gggaccccgt gaggcgacgg
    18901 tgctcaagat gcgttttggg ctgatcgacg gacgccagaa aaccctcgag gaggttggaa
    18961 aatacctgaa cgtcacgcgg gaacgggtgc ggcaaataga ggtgaaggcg ctgcggaaac
    19021 tccgtcaccc gacgcgcagc aaacatttga aaaccttgat gaactaccta aacaaataat
    19081 ccaccgcttg tttcgtcatt gataagagac aagacaaaaa cgagacacgg gaggtgtaaa
    19141 aaagcgtgga atactattcc tcgatgctga tcgtgatccc cgccatcatc ttcgcgattt
    19201 tcgcgcaatc ggccgtgagc acgactttct ccaagtactc gcaggtccgt tccgaaaccg
    19261 ggtacaccgg aaaacaactg gctcgtacgt tgttggactc gcacggccta tccgctatcg
    19321 caatcgaacc ggttccgggc gttctatccg atcatttcga tccgagacaa cgggtggtgc
    19381 gactatcgga atccaccttt cagaacgggt cggtagcggc gttgggcgtc gtcgcccacg
    19441 aagtcggcca tgtgatacag atgcaagaac ggtatagacc gatggttcta cggaacgcgg
    19501 tggtccctat cgcccaattt gggtcttctt tttcctggct catctttttt ttcggacttt
    19561 tcttatcgat cccggcgctc gttcaagtgg ggatcggttt gttcctcgcg gtcgtcgcct
    19621 tttcgctggt gacgcttccg gtcgagttca acgcgagcag gcgagcttta acgctacttc
    19681 aaacgcatct gttgatgtcc caagaagaac tgtcgatggt caaaaaggtg ctccgcgccg
    19741 cggcgatgac ctatttggct tccacgttaa tggccgtact caacctcgta agaatgctaa
    19801 tgatttcaaa aaggtcaggt gattgacaac gatgggaatt gaaacgactt tgagcaacct
    19861 cgtcggacaa tctcagaact accaatttag agtgaaggag agcgaccttt ggcaggaaga
    19921 cccctgggtg ttggacttaa aagtcctgtc ctccagggcc ctcattcatc gattattcga
    19981 gttcagccac caatttttaa tcgagcacgt cttcggagcg gaattagcct tcgccgtcac
    20041 cgatctcgag taccggcacc tcgcgccgac tcccttgggt tcggcgatca cggtgcgatt
    20101 aaccgtagat tacacgcttc gaaaccacgt ttatttccaa ttcgtggctt tcgacgaaca
    20161 cgaagagatc gcgaacggcc gtatcagccg agtcgtttta tccaaagact ttatcggcag
    20221 gaacattcag cataagctgt tatgagaaaa cctcggaaca gcgctgactt cgcggagttc
    20281 tctaccgtca aggcgttttc cgaagaagag actcaatacg atcggcacaa gggttacgga
    20341 gacgtggacg tagcccttat cttcccttcc gactacgatc aaactgtcgg taacctggga
    20401 tatcacaaag tctttcaaat actcaatacg gtagaagggg tcaattgcga acgattctat
    20461 tacgatccct cttttaccaa gttttattcc ctggattcct ttcggcccat cgacgagttt
    20521 tcgatctggg ctttttcaat ccatttcgag ctggacatct ttaatatcgt agagatgctg
    20581 agaaaaaagg gcattccgct acgaagtgtc gaacgaagag gcgaacaccc attcatcctc
    20641 atcggcggat cgctcacgta cttcaacgcc tgtcctttat gggagctcgc cggtattatt
    20701 ctctatggcg acgcggagga tccgctccca acgcttgtga agacgatcgt ccccgctgtg
    20761 gtgaaaggga agcgcaggca agaggtgtta gaaacgctat ccgaaatgga gacggtcgcc
    20821 ttgccccctt tggaaaagga gcatcgaacc ctatcggttt tctcatcgct ctcccgcttt
    20881 ccgagccgtt ccagcttcgt acacccgaag gggccctttg gacgtaaggc cttaatcgaa
    20941 gtcggacgcg gatgcttgcg acgttgttct ttttgtgtta ccggcttcac acgctctccc
    21001 gctcgcttcc ttcccttcga tatcctgaag aaagaaatcg aagacttgat cgataaagag
    21061 caggcggctt gcggaaaggc gcccgcattt ggatttatct ccgcgacccc tacggattac
    21121 ccgcacctac cagagttact ggcctttctg gagagatcct ctctagactt cggcttttcc
    21181 tcgatccgtt tggagtcgat ttccgacgcg cttctctcgg ggttaaggcg tggagatcag
    21241 cagtccatca ctatcgcgcc tgaagcggcg accgaacgga tacgccgggt gttaaacaaa
    21301 gggatcacgg acggtcagct cgcggacgcg ttggaacgca tcgcacgcgc aggctttcgc
    21361 gaagtcaagt gttactgcct ttttggaatc gaggaagaaa ccgaggagga cctactggca
    21421 ttcgcgcgct tagtcgaaga atggagaaaa ctcggattct acaaaataca tctgagcttc
    21481 aaccctctca tccccaaacc aaacacgcct tttgagagtc gagttttgga gagcgaagga
    21541 accttgcgcc gaaagaaaaa gttactggaa aacgcgctac ggggactgtg tcaaacgcaa
    21601 ttcgagagtt tgcgcaatag tcagcttcaa tctcttttag cgaagggtga tcggtcgttg
    21661 tccaaacact tggtctcggg acactccggg caaacgatcc aaaaacagct gctccagaac
    21721 gttcgcacgc aaacgcggac gaaagacaaa ggctcctctc acgctaccat tcgttgatgc
    21781 tttttcggaa tatgggccgt ctgtattcgg gtaccctaat gcgtaggaat atggaggtgt
    21841 gacgtaacat gtttccaaaa cctcgtttct ggattgtggt gatccttctc tcgttgtggc
    21901 tcgaactatc cctattcgct ctacctcaaa gccatagtgt acaaatcgcc atcccttcgg
    21961 atccggccga cacgatagac ccgcatcgcg cagccggcgc acttaccttc gagatcctgt
    22021 acaacctgta tgaagccctt ttggaagtcg accccgccgg tcagctgatc cccgttctcg
    22081 cccgttccta caccgtgtcg gaagatggaa gagaataccg gtttttactg aaggacaacg
    22141 ttgtctttca caacggtaaa agcttttcgg ctgcggatgt agtctattcc tttcggcgta
    22201 tcttggaccc tcagaccggt tacccgaaaa catcaaacta tcagatgatt gaagcggttt
    22261 tcgcggaggg cccggacacg gtggtttttc gtttgaagca aaactatgcc ccgtttttgg
    22321 cgctagtatc gaagatttat atcgtcccag atggcgcgac caaagagcaa ctggactctg
    22381 ccgcaatcgg caccgggcct ttcgtcctca aagaatgggc tagggaccaa tacctccacc
    22441 tggttaaaaa cgagaggtac catcaggaag ggctgcccta cctcgaggaa gcctattttc
    22501 ggatcattcc ggacgaaaac tcacgactca tcgcacttaa gtcaggcgcg atagacgtta
    22561 tccccaaggc ggacacctcc agtctctatg agttcgtggg aaaccccgcc tacaaggttt
    22621 tagaagcccc tatgaatttg gtacaagtgt tggctttgaa caacaaaaaa ccccctttcg
    22681 agaagcttga ggtccgccaa gcgctacaat acgccatcga tcgggacctg tgcatcgagc
    22741 tggtcgccga ggggttggct aagccccttt acacgcacct tccgcctgag gattattact
    22801 atcgtgattt ttctgcagcg tacacgtaca accccgctaa agcgaaagaa ctactcaaac
    22861 aagctggcta tccgaacggc tgccctttca ccatccaaat cgcccagctc tacccttttc
    22921 atatacgtac gggggaagtg atcatacaga tgctcaacga agccggcttc aaggcgaaac
    22981 tgcaagtgat cgaatggagt aaatggcttt ccgacgttta cggcgcccgg gaataccagg
    23041 cgacgctgat cgggcacgtg ggagaggccg atccgtacct tctgctggat cgtttctacc
    23101 aaacgtcgaa tcggaattat atgaacgtct tcattcccga gatcgaccaa ctgttggacg
    23161 cttcgttagt cgtatcggaa cccgaaaaac gtttaccttt catccatcgt attttgaaga
    23221 tactggtcga aaacgcggtc gcttactgga ttatggagcc ggaagagatc gtcatcctct
    23281 cttcgaaagt gtccggatgg gtgatctatc cggtctacgt ggacgcgttg aagcgggtgc
    23341 agaaagcccc ctgaccttcg gacttcgcat aagaattaag ggcaagtgtc acgaagcggg
    23401 cccagacgat aggcttccgc gacttctcgc tcgataggct cagggacgaa ccctcgcaga
    23461 ctcttcccaa ggatggctcg gttgcggatg tctgtggagg agaggtcgca caaaggcgta
    23521 tccagaaaat ggatacgttg gcaataagtg gacgcccact cctcgttgtg tggaggagcc
    23581 agcccggctg aacggccgaa aaccacgacc tgcgtttttt ccagcagctt ctgccagtct
    23641 ttccaatggt gtaggctggc gagggcgtcc gatccgatcc ctaagtacgg gtagtaaccg
    23701 tacaagcgat agaaacggtc cagcgtctca ttcgtataga aaacggagcg catacggcct
    23761 tcttcgtctc gataaggcga ttccagcttc agaacgctca cgcgctctat cccttcgaat
    23821 gccttttcca cccaacgcaa acgtaccgca tagggttgat tagtcccaag cccctcttta
    23881 aacggagaag cgccggtcgg aacgatcaaa caccgacgaa cccatacgta gtcgagcaag
    23941 cgctgaacca gcaagatatg gccgtgatgg atcgggttaa aggaacctcc gagatagaca
    24001 tcgttgggcc gcagtctatg ttccggttcg ataggcgccc acctcttttc ttaaggcctc
    24061 cacctctttc tgatgagaaa agagggcgca cggaccgttt ccggtttcca aaaggtgctc
    24121 ttcctcacga atacgcttga agagttcact acgcatcgcg ttttcgaaag agcaggtttt
    24181 taggttgtcc gtcgcgatat tggagaccgg acacggggtg acgtctccgt aaggggtgat
    24241 gtggatgaac cctttgcctg cagacacgca tccgccaaag gcttcttcgt ccccgggtga
    24301 gtgtatgata aacaggttct tgtgttcacg atagtacaac acgcgctgac gaaaggtaga
    24361 ccgctcttcg tccgttagca tagtcgcgta tgccataccg tacgaggtat cggggtagtc
    24421 ttcctctccg ccactcaccg gaatcatctc ggtcagaaac gcgatacgaa ccccactctc
    24481 gatcatacga tcgatgtgtt cctctttcgc ccaaaagccg tagttcagtc ggttgatcgt
    24541 tacggagatc ccattcaaaa ccccgttctt ttcaagaatc cggagggtgt ccatcgcttt
    24601 tcggtaagtg cctttcccgc gccgaagatc ggttaaggtc tcgtcgccct ctagactcac
    24661 ggctaccaac aggttgtggg ccttcttcaa atcctgaagg atcgaatcgc tcatcatggt
    24721 gccgttcgtg aaaacaacga aaaggagatg aggaaagtcg cgcaccaact gaaaggtttc
    24781 cggaaaaaga aagggttctc cgccggcgat gagaaaacag aaaactcccg cttgggaggc
    24841 ttcggcgatg atgcgtttcc attgggtgta gtctagagaa gggccaggcc ccgcgtttcc
    24901 gccagcctct tgcggttgcg aaaccgttcc ggtagcgcag gcgaagcaac cctcacaaga
    24961 aagattgcat ccggaggtaa tgctcagaat cacgaccggg gggatcagca accctcgagc
    25021 cagttgttgg gctctttttt cttctgcctt ttcgaaggcc ttgagcaagg tgaaagcctg
    25081 ccgaaaatac ttcggatgtt tcaataacca cggaaaaaac acctttccca tccgcggatt
    25141 caccagatca aacgcggata tgagcctttt tatcctcgac tgctgatttc tagattcagt
    25201 ctcgttcttt atcaatttta tctcactcct ctcctttgag ccgaatccga ctatctcgaa
    25261 aaataggccg taagcgtccc ttcttttcgc gacggcgcga tacgcttggg aacgccaact
    25321 tttaagttgg catgtcttag aaataaaaca gggttcacac ggagggcaca gagaacacag
    25381 agaaaaaatt ctcgtcaacg ccctgggaac gccgaactat agttcggcat gttctttaaa
    25441 aaagaaagac agcagagcgg gccgcgggac ttcgtgggct gggaacacca aactttagtt
    25501 tggcatggtt tataaaaaaa gcaagaaagc atcgcggcgt aagcgcccct tcttttcgcg
    25561 acggcgcgat acgcttttat gcgagctgat cgcgacgtaa aaaagacata aggcggatcg
    25621 cgggggcgta aaaaccactt accccgcgat aatcttcgta ctcttcgcgg ggtcaaaaaa
    25681 atacacttct taacccctta aagtgtcaaa tgagctctaa tgttggttcc ttcaggagac
    25741 ttttttccct cctctttttt ccattttttt gttgcgcctc gatattatac ccactcgcta
    25801 ttcaggcact ttcctctccg gccaaaaaag agcggtcgcc gggaatagat taaaatacgg
    25861 cgacgctacc gtcagaccgc ccgtcggtcc caccgcagaa aacatagcgc cccgtcgagt
    25921 ccttcgccga ccgccagatc acttgccctc tcccgaatag ggcggaggtt ggaactgtat
    25981 cgatataatg gccaaagcga gcgagctctt ttcgtatcga ctcgtcgtat ggctcttcga
    26041 aggcgaccgt tcgatctttt aaccattgcc agcggggcgc gttcagcgca tccatcgggt
    26101 gcgccttctc caccaaaagc cgtataagga gttgcgtgtg gccctggggt tgcataaacc
    26161 ctcccatcac cccgaagggt ccgatccact cacgccctcg actgaggaac cccggaatga
    26221 tcgtgtgata cgggcgatgt ttcccccgta ggcagttggg gtggcctgat tgcaacgaga
    26281 agttgcaccc gcgattgtgt agcgagatac cggtacctgg cacaactaaa ccgctcccga
    26341 aacccatata attgctttgg atataagaga ccatatttcc cgagcggtcc gccgcgcaca
    26401 aatagaccgt accgccgggg agactcgaag cggtggtatc ggatacggag tctaaggaaa
    26461 gggttttcgc gcccgcctcc aacgcggcct ttttgatata ggagcgagcg tctttaggca
    26521 tcgaccgcgg ttccccgatc gtttccgtcg cgatcttgaa cgcaagctta accgcttcta
    26581 tcgacaagtg cgtgcgctga acctccgaaa gggtttccag ggagaagggc tcgagaaacc
    26641 ccaaggccat caatgccacg atcccctggc cattgggtgg gagctcccaa acggtatacc
    26701 cacgaaaatc gacgctcaac ggctccaccc attcggcttg atagtcggcc aagtcagaag
    26761 actcgagaaa tccgccaaac gaccgaacgc agcgaacgat ctctcgagaa agccgaccgg
    26821 tatagaagga atcggttcca gtctctgcca gctctttcag ggttcgcgca tgggcattag
    26881 aactccacag ctcaccggga aaaggcgcgc gtccagaggg cgcgaaggtc cggaaccatt
    26941 ctccgaaaag cgaagtgctc ggttgctgtc gataggtttg gaatgctttt tcccaacctt
    27001 ttcctgtcaa aacagaaacg gggtatccgt tttgagcgta ctcgatcgcg ggttggaaca
    27061 atgcgtcgaa aggcagaacc ccgaatcgtg aatgaagctc tttccacgcg cgaggggctc
    27121 cgggaacggt taccggaata ggcccataac gcgggatttc ttggagtccc tcccgtttca
    27181 acacctcgag cgataggcta gccggagcgt acccgctgct attcaagccg tacagctggt
    27241 cttcaaacag gacaatggcg aaagcatccc caccgatccc gtttgatgtg ggctcgacca
    27301 cggtaagcgc gatcgcagtc gcaaccgcgg cgtcgatcgc gtttccgccc tgctcgagca
    27361 tccgtaaacc tgcttgcgcg gctaaatact gcgaagttgc cacaatcccg ttcgagccga
    27421 ataccgggta ttgtattttc ggccgccggt tctgggtcac gttaaatgga aacattcttt
    27481 ccctccctct agagaagcga tcttttcacg ctattatatc ccttcttagc ctaaaagagt
    27541 caccaacgcg tggaatagcc ttctcgtcct gcgcgacgcg gctccactat ccaccccgtt
    27601 atcatacccg agcagagtgc catcgatgaa aaagcgcgga ggaaagacct gtgggcaaaa
    27661 agccctagtt tgcagaatat cggaaggaac atcggatcaa attcccggag gggtggcgtc
    27721 caataaaaat agagggtggt cgagggaaat tcggatcgat cgcggctttc aatcttcctt
    27781 gtggtcgtat aagacgcgtt cgacaaagag gcccttctga cgatccagat aaatgagctc
    27841 ttcctcttgc tcgatgtcag ccacaatcgg ggtggtgagc gcgttcttcc ggttcccctt
    27901 cacgacgact tcccgaccat tcgacaacaa caagcgggtg ccgaccggat atatcccgaa
    27961 aagccgaaga aaacaggaag cgatataggg gtcgaagatt tttcccgctt gctggatgat
    28021 ataaccgatc gcttggtaag gagaccaccg catcttgtac acccggtcgc tcgtcaaggc
    28081 atcgaatacg tcggcaatga cgatgatgcg cgcgctttgc gggatttctc ttccttttaa
    28141 cccgcttaaa taaccggatc catctatgcg ttcgtgatgg tagaggatct cgtgtaagat
    28201 aataggattg tctatccccg aatccaaagc catttgatga ccgtacagga cgtgtttttt
    28261 catcagttcg aattcattgt cggcgagact cgttcgtttg ttcaatagct ctttcggaac
    28321 gacgagtttt ccgatatcgt ggatcagagc gcccgcgacg atcgcttcgg ctgtctcccg
    28381 atcacccgaa tagacctcga tcgctatcaa ccccgcaata atcgaaacgt tgacttggtg
    28441 gcaaaaggta tactcatcaa aagtgcgcat ctgagagaag aaagggacga ccacatcccc
    28501 atagtttgac aacatattcc ggaccacttg ccgagagagg gtggatacct cttcaaccgg
    28561 tatcttctgc tccctttgga cggcatcata gaccttcttt accccttttt ccatctcctc
    28621 tagtacttca gggctcagaa tcggcttaat cggcgattta taggtctccc ttgactccgt
    28681 caatatccat tctattccgt gacggaccaa cctctctttg atcgaaacag agaccgtttc
    28741 gcccatatgc acgatcgttt ctccggtttc gtattcggtc aatgtttccg cgataacatc
    28801 tcctgcgcgg atttctgatg tgcgtttcca ttcgagcatt tttacccctc gatttcttta
    28861 tcggtgtatt cgttggcttg agtcttcagt aggatgaatg cccctttatt atacacgttt
    28921 tttgagcaga tatgaagctt tcacgacata ttccaactga gttaactatc cgatgccccc
    28981 acccactaca accctgatgt gaagccaaag acccacttgc gcgttcttca gaccgcctcg
    29041 aaaaaaaagc caagatgatc actaaactgt aaattttacg agaacgatgt gatttgagta
    29101 gaaatacgct ataatctacg gcgatgagga gagggggata gctggaacac agatggaata
    29161 cgaacagata aaggcgcttt accggttggc gaaggaattt ttgtctgcgg aacccgtgga
    29221 cttactacgt accttgaaaa aaagcgtgag aattatccat tctctattcc cgcggtgcag
    29281 tctgtggttg tgggtgatcg gaagggagcg agaaccttgg acgtgttatt ggagccactg
    29341 tcacacggtt ggggaccagt cccgatggcc cgacgggctc gaaggccctc tttcaaacgg
    29401 agagcgaggc ggtgaaaaag cagcggatcg accgggtatc cgtatcatat cggagggcaa
    29461 aacaaaagcg cgggtcttta tcccccttga acaccaaaga cgtgcgttag gatttctcga
    29521 gattctccat aacaaaacgg ttaggataga agaaggggag tggatggctt tttaccgtga
    29581 gctctcttcg attttctctg gggtccttct gcgcctaaac agagcccccg cggaagagat
    29641 cgatctttct cggattcggc tccccggaat gatctgggaa accgaaccgg atggccgata
    29701 ccggtatgtc ggtccgaggt cagaagaatt gtatgggtat ctgccagaag agatgatcgg
    29761 gcaaccacgt caggattttt ttgaagggcc ttcagagacg attccctcag atctggaacc
    29821 tcccatcgtt ccccggacta atgaaatgtg ttcgaaatat gcccgggtga aacgaaaggg
    29881 gggcaaatgg cagcagataa tcgccagcga gatgcctcta ttcgacgaat ccggtaaatg
    29941 gagcggcttt cgtggcatcg atcacatcta tcccgaacct tcatcaacgg gcccctttcc
    30001 cggtgtaaac caagagttgg aacgccgcgt gaccgaacgg acccaagcct ttcagcggat
    30061 gatcgaacga tacaagaccg ttcaaatgcg actggagatg gtgttatggg ccgcaaacca
    30121 agggatttgg gattgggatt tgcagacaga cacgatctat cacaatgaga attattacac
    30181 gcttttgggt ttcagcaaac gcgattttcc cgatccgaat aaggtatgga ggcagctaac
    30241 ccatccggat gatatcgatc tcgtcctaaa agctttagac gatcattact ccggcagcaa
    30301 gccttactac gaagtcgaat atagaatgag aaagaagaac ggcgaatacg cgtggattct
    30361 cgataaaggc cgtatcgtca gctatggcga gaatctcgtg ccgttgagaa tggtgggggt
    30421 ccattccgac atcacagaaa ggcgcgcctt cgaagaggag ctaaggcaag caaaacaagc
    30481 ggccgaagac gccaatcgca gcaaatcgga gtttatcgca aacgttaacc atgagctacg
    30541 gaccccttta acggtgatta tgggattggc tgaaacgctc ttaatggaaa tgcccgataa
    30601 caagaaggag ttcccaatcc atatcctgaa aaacgctaaa cagctattgc ttttaatcaa
    30661 cgacatcatc gacctttcga aaatcgaatc cggaaagttt tcattagcga agaaatcgtt
    30721 ccaactcgcg gagacgttgg actatatccg ccaaagtttt gcgctagaaa cagagaaaaa
    30781 aggaatatcc ctaaaaatcc tagggccttc catagtcttg tatggagacc acgtacggct
    30841 caaacaaatc ttgacgaacc ttgtcgccaa cgcgataaaa ttcacatccc aaggcggcgt
    30901 gacccttacc tgtgaaattt cctccgaaga agagagcaaa gtcgtcctcc tcttcgaggt
    30961 tcaagatacg ggtatcggca ttggagaaag cgaactcaac aagattttcg aaaggtttta
    31021 ccaaatcgac gggtctaatc atcggaaata ctccgggaca ggccttggcc tttcgatagt
    31081 caaacagctg gtgagcctta tgaacggaag cgtctgggtg aaaagccaaa tcggtgaagg
    31141 cactaccttc tacgtccgta tcccgttcgc gttgccgaac cgtcctctaa aaccctctac
    31201 gaatacggaa cgagaagtaa ctacggaata aactatggac tgtggcgcct tacagatgtt
    31261 gtttttttct accccgtggc cgcttttcta actcaccgag tcttcttttt tcctttcgcc
    31321 ccgtggaagt ccctttgaga agaacggaat cgccgttttc tttgttttcg attccccatt
    31381 tgtgatatat ttcctcctga gagaagaaaa cacacatttc gttgtttcta agggggtaag
    31441 gcaaatgaat aagaaagaga tggtggacaa gctcgcattg gagacggggg tcacaaaaaa
    31501 agaggcggct cagtttctcg acgctttcgt ggacgtcgtt tccaacgaat tatcgaatgg
    31561 cggaaacgtg aggctcgtcg gtttcggcac cttcatggtc aaaaacacag tcgaacgcaa
    31621 tggcgtcgat cccaggacca aaaaacccat aaaaatcccg gcgcgaaaag tacctaaatt
    31681 taccgccggc aaagacttga aactcagggt caagtaaaaa agcggggaaa cccgcttttt
    31741 tacttgactt ttcctcaccc gtatgctaaa ataggcctga aagttcgggt gtgtagctca
    31801 gcgggagagc gcttccttca cacggaagag gtcgtaggtt caaaccctgt cacacccacc
    31861 aaaaaaagag acctttttgc ggtatgtttg tcgatatgaa gggtctcttt ttttgtggga
    31921 aaaggtctga aatccctgat ttagacccga acctatgaac gcagcgactc gctggtgggt
    31981 ataatatcga gacgcaacaa ataaacggga agaagagagg gaaaagtata acccccgaag
    32041 gagccaactt tagagctcat tagaccctta caggggggta aagaagggta ttttccgatc
    32101 ccgcgaagag tacgaagatt atcgcggggt aaccagcggg ctgacttcga aagcccgcgt
    32161 agtccggtga tccgctcttt gccccttcta cgccgtgaag ctcgtgtgtg ttatcgcggg
    32221 gtaagtggtt ttacgacccc gcgatccgcc ctatgccctt ctcaaaagcg tatcgcgccg
    32281 tcgcgaaaag caggggcgct tacgccgcga tgctcgtggg tgttatcgcg gggtaagtgc
    32341 gttttacgcc tccgcgatcc gccctatgcc cttctcaaaa gcgtatcgcg cccacgcaaa
    32401 aagaaggggc gcttacgccg cgatgcagcg cacgggaata ctcccagtga aaagataaaa
    32461 ccacgccaaa ctcaaactaa agtttggcgt tcccagggcg ttcacgagaa tctttctctg
    32521 tgttctctgt gccctccttg agaaccctgt ttttcacgac atatacaaaa gcgtatcgcg
    32581 ccgtcgcgaa aagagcgggc gcttacgcca cgacgactat atggactaaa aatcgtgcca
    32641 tcgattctgg tcttgtttct tgatggcgcc ttataacttg acccgcacgc gtctcactca
    32701 gtttctattt cacttcgatc tcgccgatca tctttctctg gcgaacgggg tccttgccgt
    32761 actcttccga agcgctttcc gccaagtcgg ccaacaactg ggcgaactcc ggatcatttt
    32821 tcttgaggtt ttcgatccgt tcgttgaact gttcaagacc caaacggtac tgtagccggt
    32881 cgaagccttc tccgtaagaa gcgccccgtg tctccatatc aagaatgtat ttttgccagc
    32941 tctcttccca aatcgggtcc agatattccg cggcagaagc cgaatacgcg tccaaatcgt
    33001 cggccaactg cgtaaagatc acctcggccc cttcgtgggt cggacgggcg gcatgctttt
    33061 tcacaacgct ccgcagcact tccggattgt cgatgatcat acaagggcga agcaaattgt
    33121 cggtatgggg ttggtgcata cgaatatccg cgaagaacgg ggattgaagg catttcacca
    33181 aagacttgtc tcggatgtta tcggtcgcga aatgcgcgaa aatgcagggt tccacgtctc
    33241 ctttgtggtt gatatggata taacgtctcc ccccggcgat acagccgtga acaaacgggg
    33301 cgtccgccca aaagtccatg atatagaaag gttgctcgga gcggaacttt tggatgaatt
    33361 ctccgtaacg gtaccgttgt tcaggcgttg gcatcaaatc gacggacgcg tcctttccaa
    33421 ccggcataaa caagaaattc caagcgtaaa tgaccccgcg gtctcgccaa cccagataga
    33481 actctggatt cgtcataata tcgaaattgt gtttggttaa cacgagggac atcccgaata
    33541 aaagattgcg ctctttcatc aatttcatag acgcgagcac cttttcataa acgcctgggc
    33601 cgcgcatgaa ttccgtttct ttggcgtcgc cgttcacact aaaggcgacg acaacgttct
    33661 tcagctcctt caggcgatcg gcaacctcct cggtaatgag tgtcccattc gtgaaaacct
    33721 gaaacaaaca atcgctgttg tcctcaaaga tatcggcgag ctcccaaaac cggacgaaag
    33781 gctctcctcc taagatcgtg aagaaaaacg tcccaaggct cttcgcttcc ttgataattc
    33841 tccgtaattc atcggccgaa aggtctccct gttgcgcgta ttcacccgca taacaaccct
    33901 gacacctcaa attgcatcgc atcgttgggc tgatcagaat cgtataaggc gcagtgatcc
    33961 cgtgtttttc catcaactct tcgttattgt ccatccagcc caaactgatg ttcacgaaca
    34021 tatttttcat aaaggtgcgt acttggttct catctctgga aagcagctcg atgaaccatt
    34081 gtttgctgcc agggttcgcg ttcgcccacc ctagaaattt cgtaaacttt ccgccggaag
    34141 aagagaagag gctctccagt ttcccgagat tggcagttat tttctcaaaa ttgttttgcg
    34201 ggtccttgtg gatatacttc acggcttggt cgatgatcgt accgatcgca atcgacgaaa
    34261 ccttgtcttt catagacttt cctaaaccca tggttatcct cctcgatgat atgagactca
    34321 ccccattata tttttctccc aattttacca cttagtcaaa ttttgtcaag actcgattat
    34381 tgaccgtttg gtaaaatttt aatgaccgcc cgtcattttt ttaacggacg taagtataga
    34441 gagtccaacg cgtgctgtaa tgcgctttcc cgtcagtgat taggtatgct gcggccccga
    34501 ttcgagagag cgtttgaaat aggatttccg agtcaagtgt ttgagccgcc actacgaaac
    34561 aggccgcctc caacgcggtt ggcgcgatga ctgcggcgta ttctatcggc tttatcggtt
    34621 ctcctttatc gggtgacaag agcagatggt cttcataggc ggccgtccct tcaggtggag
    34681 tcagtgtcgg atggtgaaac aaagctccag aagaaagata gagttcttga ttattccatc
    34741 cgcccgcgat ccaatcggcg ccccccgcct taggcccgaa aaaccggata tcgccgtcta
    34801 tctcacaata ccctgtggct ttcggatcgg ttttccgaat cgactcgtac acgcaatcca
    34861 acgcgtagcc cttgaccgct tttccgaaaa agaagaggaa aggatgatcg gagtggacgc
    34921 tcccgtcttc gagattgagg tcggtgggat agcgataagt ttcgagtgtc gggagttcaa
    34981 agagagcata tgggcgataa tgaatcccga tggtgaagtc ataatgccaa agggtttttt
    35041 cggagaggtc ctcaagtttc gtcagaaagg cgtacgtttc tgcgtctgtc tttctattcg
    35101 tttctgaaat gcggttcaac cgggtcgttt cgctttccgg tgaaaagaag gagaaacggt
    35161 tgaagacgcg ttctaactcg agttgcgccg cacgcagcag ggtctcgtgg tctccctggc
    35221 taccgagtac caatcgcacc gcccggcctt gtaccgaaaa ggcgtggtcg gagtaagggg
    35281 tctgggttcg aaggaccata tacgcgacaa tacagaccaa aatgaaaatg acaaaactga
    35341 cgaaaagccg atttcgtttg gaaaggcggc ggaggtccat acccgtatac cctttttgct
    35401 cctcatcagg agagccgggc ggatcggccg aaaggttttg ctgcgatagg ttttgagaca
    35461 tatagacggt ctagtgccct aacgacctcg gatcgaaggc gtcccttaag gcgtctccaa
    35521 aaaggttgaa agccaaaatc gcgataaaca ggaacaaacc cggaagcagc agccaggtgt
    35581 aattacggag cgccacgagg tcctgcgctt gcgccagcat caacccccaa gaagactgag
    35641 gttctcgtat accaaacccc aaaaaggata accccgcttc tcccaaaata tatcctggga
    35701 tgctcaaaga ggctgctacg atgatatagg tggccgtatt ggggatgata tgcctccaga
    35761 taatgcgaag ggagggatac cccatcgctt tcgccgcttc gacgaactcc ttctgcttca
    35821 aggatagtac catcccacgg atgacccggg acatccctgg ccagccgatg aaggagagga
    35881 tgacgacgac catcaaatag gtggtcgtgg aaggtaaatc aggaggtaaa atcgcacgca
    35941 acgagatcag aaggtagagc gtcgggatag aaatgagaat ttcggtgagc cgcatcaaga
    36001 tttcgtccgc ccaccctcca tagtaaccgg aaatccctcc gaggaaaagg ccgatagtga
    36061 aagtgatcaa aatccctatc aatccgacag acagggaggt tcgccccccg aaaaggatgc
    36121 gggtgtagat atccctcccg tagttgtctc ccccaaacag gtatagaacg gccggagaat
    36181 ctacaccgaa caggtgaagg ttcgagggga tgaagccgaa cagtttgtac tcccaagatc
    36241 gaacgaacca tttgatcggg tacactttga aatcatattc ttcgagagcg ataagagaga
    36301 tcgattccgt ttttccatcg gcgtatttca ccgagatctt ttgtagagat tcgacggttt
    36361 ttatttgtgc gaggtcgccg atttgctgta atgttttcac atacctggga ttgcttcggt
    36421 ataggtccaa agtgtctttt tcgacggaga cgttcacgag cggttcttta aaaataccct
    36481 ctttctcgat tctcaccata ttccaatctt tggtcgttgt tttgaatagt ccctcaccag
    36541 tagctccaac gccaatttca tctattttca cttgcgcgac gagcgcggtg acattgtttt
    36601 cgcctaatgt gtaaacgaag ggtgacccgt tggttccgac ccctttgacc atacgcaact
    36661 ccgaagtctc gtagaaagac cgctgatagg tgatagggtc tcgagccgtc tcgagcggga
    36721 agatatacgg gccgatccat tctcccttat gtttccagta gattttcgaa ggggcggcaa
    36781 acccgatact cgcgttttgc ttgtaaggac tcatcggcga caaaaaatcg gcgaagatca
    36841 cgatgaggta gaagaaagcg agtatccaca tcgaaaggat gccgagtttg tgctttttga
    36901 atgaacgcca cagaagcaca cgcctcggta aaaactctac ttcgaaagat tccccaacct
    36961 gatcgaccgt caggttctct ttcgtattct ctttcgtatt ctttttctgt ttatccatag
    37021 cctcacccct aatggaaacg gtatcggatt ctcgggtcta cgagcgctaa aagaatatcc
    37081 gcggctaagt ttccgataat gagcatcaac gaactgacca tcaaggaagc catcaccaga
    37141 taaagatcct gggcggtaag cgcttggatg accaaacgtc ccattccggg ccatccaaac
    37201 acagtttctg tcaagacggc tcctcccaac aaaccggaca aagagtaacc aaatatggtg
    37261 atcaatgggt tgatcgcgtt acgtaacgcg tgcttataaa aaacgacctt ccgcggcatc
    37321 cctttggctt tggcaaattc gacgtaatcc atatggagtt gatctaagaa ttggccccgc
    37381 atctggcgca taatcccggc caaacccgaa aaccctaaag tcaccgccgg cccgatcacg
    37441 tgccaaaggt aatcgacgat caactcccag gtattcatct gcgagctgtt gaccgacagc
    37501 attcccccga tgggaaaaag tcgggtccga gaggcgaagt atagaaatag tagagagaaa
    37561 aagaagctcg gaatcgaaag ccctaggaag gcgatcacag aagacagctg gtctttgaag
    37621 ctgtattgtt tgagggcggc gtagatgccc aatggaatac cgatcgccca cgaaaaaacg
    37681 agggttgtca agctcaacag taacgtagcg cccaatcttt gccagatcag gttgaacacc
    37741 ggctgcttga actgaaagga atacccccaa ttccctttta ccacttggaa caaccaggtg
    37801 aggtattgtg tgaacgggtc tttgtcaaac ccgaactccc gttccaaccg ttggacggtt
    37861 tctcggctga tcgaagggtc tagccggaac acatctagaa agtttccggg agccgccgaa
    37921 atgattagga aggaaaggat agacactccc agcaaaacgg gtatagacag gatgagccgt
    37981 cttatgatga actttaacac gtcgatttta cctccgaaag agaaatgggg gattctcgcg
    38041 aatccccctg aacaggtagg ttattctcga tacagacccc agagattcca caggatcgga
    38101 ggcgacgagc tgctgccagg atccgtgccc ttcaactccg cctttttcgc gtacagtctc
    38161 aacgcttgga ccgtgtacac gagcggcgct tcttccacga agagtctttg atagtccgcg
    38221 aacatctgcc agcgtgcctc ttcatccatc tctctcactt ggtcgcggaa aatccggtcg
    38281 atttgccatt cttcgttaga gatccaatag ttgtcggggt cggcccaagt tccgacatcg
    38341 ggatggatgt tccagaagtg tagatccccg tcgatgcgat tgacgttgga tccggagtgt
    38401 ggatccggcc cgccggtcaa gcccatcacc atcgtttccc agttcccggt ggcagtcagg
    38461 gcgcccacca aagtgttgaa gtcgatcggg ttgaaaatga catcgatccc gatctgtccg
    38521 aaggtgtcga ccatgaggtt acaggccgtt tctctttggt tgttaccggc gttggttgtc
    38581 agaaggaact tgacctggat accgcgatca tctatgagtt ttccttcggc gttccacgtg
    38641 aagccggctt ctttgagtat cgcccttgat ttcgcaatgt cgaaggttgc ggccaagtct
    38701 tcgacggctg gatcgtagaa cggagacgcc ggagagacag gcccccattg cggttgcgct
    38761 aaaccgttgt aaatgacctc aatcatagtt tcacgatcga tcgcgcagga tagcgcgttt
    38821 ctgaattttt tgtttctaaa ccagtattgt tgctctttcg tgggagagtg ttggttgaac
    38881 gcaaggaata acgtggaata agtcgggccg caaatgccga cgtcccattt cttcgccgcg
    38941 gcttgagctt ttaggttggg gtattgggcg gcttgagggg ccaagtaatc cagttctccc
    39001 gcttcgaact tcaactgggc ggtgttcaag ttcggcacag aaatgaatat cagggtgtcg
    39061 aagtagggca gccgattgcc cgctgggtcg actcggaaat aataaggatt tctcttgagt
    39121 acgactcttt ggtcgggcag gtattcggaa acgagcattg gaccagaacc gaccagctta
    39181 ttgacatcag ccaacgtcca gaattctttc cattttccag ccgctaaaac gggttcgacg
    39241 atgtgcttgg ggaaaacttg aacgcccccg atttgtcgta atcccacagc gtaaggctct
    39301 cggtaccaaa tcttgactcg gttttcgtcg agcttttcga tttggggggg tctgccttgg
    39361 gcatcggtga tcacgtcgta agtgctcaag cagttttcag ggacaaacca gatgtttttg
    39421 aacgtccagt acacgtcttc tgccgtcaac gggtgcccgt cggaccattg gagacctttg
    39481 cggatcgtga aggtccaccc gttaccttcc ggggtgactt cccaatctaa cgcgaggttc
    39541 ccgcctctga tatcgtcgtt ctttccgaaa tcgaccaagc tatcccacat aatgccggtg
    39601 atgtcggtag agctcgtttc ttgggcgaag gccgggttga aggttttcgg gtcagagata
    39661 gagtagtaat aaagggtccc tccaaccttt ccgtgaacgt cttcgacgat gtactgcagg
    39721 ttttccgaag gaacgatacc cgcgaatacg atgccgagta ggaaacagag cacaagcaac
    39781 acactaaacg cctttttcat gtttttccct cctttgattt ttgggagtac tgcgctgatg
    39841 cgcattcttc tatttttcta caattctgtc tggttcgccc aaagagtaaa tatgacaggc
    39901 gacccaatgg tgttcggcga tgcgaaccat ctcggggtac tctttttcac aagccggcat
    39961 cgcatacttg catcgcgtcc ggaaaaaaca tcccgatggt cggttgatcg ggctgggcac
    40021 ctgtccggtg aggagctctt tccgttttcg gcgcgtcttg gggtctggga tcggcgtggc
    40081 ggccaacagc gctttggaat acgggtgctc cggtttgtcg aagatctcgt tggtgtcccc
    40141 tttctcgacc attttcccga ggtacataat acctacttgg tcgctgatat agcgaacgac
    40201 cgataagtta tggctgatga agatatatcc cgcgttcagt tttccttgca actctttgag
    40261 cagcttgatg atctgagcct gaaccgaaac atccaatgcg gaggtcggct cgtccaagac
    40321 gatcagagac gggtcgacgc agatggcgcg cgcgatggct atacgctggc gttggcctcc
    40381 cgagaattgg tgcggatatc ggtcgacatg gtaaggtttc agcccgaccg acgacaggag
    40441 ctggctcatc cgttcttcgg cttccttctt cgaatgaacc atttcgtgga aaagaagcgg
    40501 ttcggaaagg gtttggccta cggtcattcg cgggttgaga gaagaggtgg ggtcttgaaa
    40561 cacgatctgc atcttcttgc ggtagtcttt taatggccgc tctttgagag tggcgatgtc
    40621 ttgcccttcc aagagtatat gcccacctgt cggattcaat aaccggatga tcgttcttcc
    40681 gatggtggtc ttcccacaac cggactcgcc gacaatcccg aaagtacggc ctttgtcgat
    40741 cgcgaagctg acaccgtcaa ccgccttcaa ctgcgcaacc gttctttgaa tgagaaaccc
    40801 cgatttaatc gggaaatatt tccgaaggtc cttcacttcc aatagggctg tagagccggt
    40861 ctcaggcatg tttgcctcct cgcctgctgg cagagcttct ctctataggg ttgaaacacc
    40921 ggatgtagtg atcctccata cggatcagcg acggtagctc tttcaaacac cgctcgatct
    40981 ttctctggca ccggggaaga aaaggacaaa gattgggcgc gtttagcatc atcgggggtt
    41041 gacccggaat cggttctaaa acctggtccc gacggtccat cctcgggata cagctgagta
    41101 acgcgtgtgt gtaggggtgc attggatttt cgaagatcgt atagacatcc gtatactcca
    41161 tcgtttttcc gccatacata accataactc gatccgacat ctcggcaatc acagccaaat
    41221 cgtgggtgat gaagatcaat ccacttctga attcggcttg aaggtctttc ataagctcca
    41281 atatttgagc ttgcaccgtg acatctaacg cggtggtcgg ttcatccgcg atcagtattt
    41341 ccgggttgca ggagatggcg atcgcgatga ctactctctg acgttgaccg cctgaaagct
    41401 cgaatggata ctggtgaacg cgttcgcggg gattcgaaaa acctaccttg tccaacatct
    41461 caacggcgat atccaaggcc tctttttcgt tcaccttctg atggaacata atcgtttcgg
    41521 tcatctgatc ggctaccgtg tagagagggt ccaaagaggt catcgggtct tgaaagatga
    41581 tgctgatatg ctttccgcgg atcttcgtgt attcgtcccc ttgaagctgc gccaagtcta
    41641 gaaacccgtc cttttgccgt tctggaaact cgggagtttt gaagaaaacc ctaccgcccg
    41701 ccaatcttcc cggttttgcg atcaaaccaa cgagggattt tgcggtgacg ctcttccctg
    41761 aacccgtttc tccaacgatc ccgatcactt cgttttcaga tagtgagaaa gaaacgtcgt
    41821 ctacagcttt tactgtgccc tcggtaaggt cgaagtacgt tcgaagctcc tctactttca
    41881 atatcggttc attcaaatca gacacctcgt cagcactatt cggtttggta attattacga
    41941 cacatagcgt taggcaatta tagcacggtt ttttcttttc gtgcacaccc gttcgtaaac
    42001 cgaatgaatt cacttatgga aagctcccga cctcggatag aaatggccgt ggctatcgtg
    42061 tcaactccgc tctacaaacg gatctttctc gaatcgacaa acttatccag cgcatttcgg
    42121 gacctttcac cacctctcaa actcgggcgc attcgagcat tttgacgcag ccgcttgtga
    42181 tataataggc cttaagtttc aggaaactcg aactcttttt tagcgcggga gttaagtttt
    42241 cgggagggct gagcgggatg tacgacttta gagcgatcga aaaaaaatgg caagacattt
    42301 atgcagcgga ggagccgttt cgaatagatt tagaaggagc gaagaagccc ttttacaacc
    42361 tgatgatgtt tccttatccg agcgcttcgg gtttgcacat cgggaatatg ttctcgttca
    42421 tcggttcgga cgtctatggc cggtatatga aactcttggg atatgatgtt ttcgaaccga
    42481 tcggctttga cgcgttcgga atccacagcg agaactacgc cttaaaaacg ggaaggcacc
    42541 cagccgagct cactcctgaa agcatacggt ttttccggga ggagcagctg aaaaaaatcg
    42601 gggcgatcta cgactggcgt agcgaagtga acacctccgc tccggaatac tatcgttgga
    42661 cgcaatggat ttttctaaaa ctgttcgaag cgggattagc cgaaaaacga gaaagcgaag
    42721 tcaattggtg cccttcctgc aagacggttt tagcggacga acaagtgatc gaaggacaat
    42781 gcgagcgttg caacgccact attgtgaaaa agacgatgag tcaatggttt ttcaaaatca
    42841 cgaaatacgc ggaccgcttg ctcgaaaact tggaaaagat cgactggaca gagatcacga
    42901 aaaacattca gaaaagctgg atcggtcgtt ccaaaggcac caccatttct ttcccaatcg
    42961 agtcgacgga cgaacagatc gaggtcttca ccacgagggc agacacggtc tttggggtga
    43021 ccttcctgac tttggcccca gaacaccccc tcgttgaaag aatcgtgcaa gaagagcgga
    43081 gagaggccta tcaggaattc ttatctcagg tcgccaagat ggacgatgcc acgcgcacct
    43141 ctatcagtcg atccaagacc ggtattttca caggcgcata tgcccgccac ccctttacga
    43201 acgaaaaaat cccgatttgg gtgggcgatt acgtgctgta cgcttacggc acaggcgcgg
    43261 ttatgggcgt gcccgcacat gacgaacgcg actttctctt cgcgaaaaaa tacgatttga
    43321 cgatacgaaa ggtgatcgcg tgctccgatg atcaattgcc ctataccgag aaaggggaga
    43381 tggttcatag cggagagtat tccgggtggg caagcgagcg cttcatctcc caagccgaaa
    43441 ccttggctcc tttcttgaaa agtaaagtac agtatcacct tcatgattgg tgtctgtccc
    43501 ggcaacgcta ctgggggcct cctattccga tgctttactg cgaaaactgc ggtaccgtcc
    43561 cggtgcccga aaaggacctt cccgtcttgt tgcccgtcac cgatgactac atcccagacg
    43621 gtagcggtct atcgcctttg gccaggcacg aagccttcct atgggcaacc tgccccgttt
    43681 gtggagggaa agccaggcga gaaacggatg tcagcgacaa cttttttgat tccgcgtggt
    43741 actttttacg gtatgtctct ccggacgatt caaaaaaggc tttcaattcc gaacgggtgc
    43801 agaaatggtt gccggtcgat atgtacgtgg gagggaacga acacgcgaat ctccatctga
    43861 tgtacgctcg ttttatcacg atggctttgc acgatatcgg gcttctacct ttcgaagagc
    43921 ccttcacgaa gttccgagga cacggtatga tcatcaaaga cgggcaaaag atgagcaaat
    43981 cgaagggcaa cacggtgaac ccgaacgatt acttcgagac tcacggcgtc gataccctcc
    44041 ggacttactt aatgtttatg gggaatttcc tagagggagg tgatttccga gaaagcggtt
    44101 tggacgcggt aaaacggttt atagggcgag tgtgggactt aaccgctctg ccgtccggtc
    44161 cgagctctct gaaagccaag atcaagatgg ctgaaacgat caagcgagtt cagttcgcct
    44221 tcgaaaacat caaatataat accggtgtcg ccgcgttgat ggaactcgtc aacagcttgc
    44281 aggacgaaca agcgctcgaa ccgagcctta tcgtcgatat cgcccgtttg ctggcgccgt
    44341 acgcgccttt catcagcgag gagatccatg cgatgtacgg ggggaaaggg accatactca
    44401 atacccgctt ccctaccgga tacgacgtct atctcgaaca agccctggcg gaaatacctg
    44461 tgcaaatcaa cggtaaattg aggggaaaga tcacgatcgc acaaaacgcg acccaggaac
    44521 aggtgatgga aaaggttctg gaagacggga ggctaaccgc ttattttcaa gggcgtgagg
    44581 tgaaaaaggt catctacctg caagataaaa tcatcaacct gatcctcggg tctatgtaac
    44641 gattgttgag aaaccgatga acccttcttt ggataaagcg tagatcaaaa cgccgataaa
    44701 cttgaaatag ctggctgaat ggtgtatact acagaacggg aagttgtttc ctattctatt
    44761 ttatctgttt tctctcttga tggtatgtga gttgtccgtt tctatgtggg gggtttaagg
    44821 aatgcttttc aagcgttttt tgtgttccga ctttcgagaa aatgcggaac ggttctttgt
    44881 tacagaagac acatacgcgc tgtgcgccta ccgtctttca caacaagaac gttgcttgct
    44941 tctcgatatt tgccttcctt atctgacggc tttaaacttg tctgagattc gcatccctat
    45001 gaacggcatg cccaatgagg gctttctgtt cttgggagaa cgacctcgat gggagcagac
    45061 gatctggaag ctcctgaggt ccaggtcaga cctcacgatc gtcgtactaa cccaaatact
    45121 cgagcttctc tacaatagaa acctcagacc gataaccctc ccttcaggaa aacaactttc
    45181 tttcgaaaga cctctattca tgggggttat caatcgaacc acagacagtt tttatgctca
    45241 aagtcgtatc gaaagcgacc aagaagcttt ggagaaagcc ttagagatgg aagagcaagg
    45301 cgcccaactc atcgatgtcg gcggagaatc ctcccgcccc ggctctttgg gcatccccga
    45361 acaggaagag gaagagcggg tgatccctgc gatccggtta cttgcaaaac atatcaaaat
    45421 accactctcc gtggatacca ccaaggcgtc tatcgccaaa aaagctatag acgcgggcgc
    45481 cgatatcgtc aacgatatct cgggtctatc gccagattcc gagctgttcg aatacgttgc
    45541 gaatcagaaa atcccggctg tgattatgca tatacaaggg actcccaaga cgatgcagac
    45601 gcgcccgact tatcggcacg tcgtacggga agtccaaggg cgatttgaac tcgttttaaa
    45661 cgcgttggga gaaagaggtt acccgctcca tcaagctatt ctcgatcccg gcttcgggtt
    45721 cggaaaaact cttcccgacc acctctatct ctggtccgaa cttgccagtt ttttgcgctt
    45781 tcgattgccc gtcctcgtcg gaacttccag aaaatcattt ctcggcgcga caaccggtac
    45841 ctcgacacct gccgaaaggc ttcccgagac cctcgcgacg acggcctccg gtatcgcccg
    45901 ttccgtaaat atctttcgag tgcacgatgt gaaggaaaac tttcaggttg ccaagatggc
    45961 attttttatg atgggttttg tatgtgaagg ggtcaacgaa aggggggttc atagtgaaac
    46021 tcgctgaagt gaaggccgtt ttggaaaaaa acggactgtt gctcagtata gcgaattatc
    46081 aaggaaacga gaatctcgaa ctggattccg tcagtatcga cacccggaat atcgttccag
    46141 aaacgctatt tatctgtcgt aaaggcgtga aattcgattc gcatgccctc gtaccggagg
    46201 ccgtacggct cggcgccaaa gcgctcatcg tcgaaaaacc ggtagacgcc gaccagattc
    46261 cgatgttgct ggtatccgac agccggtatg cggaggcggt cgtttcttcc gcttttttcg
    46321 acttcccgca cgaaaaattg agagtatatg gcatcacggg tacgaatgga aagacgacga
    46381 ttaccacgct catccattac gtcttaaccc gaaaaggaaa gaagggatcg ctgttaggca
    46441 cggttaaaaa catcatcgtc gaccgttact acggctccga acacacgacc cccggagccc
    46501 tggaggtctt gaaagaaacc cacgaaacga tagccaaagg tggggagttt ttgagtatgg
    46561 aagtgtcttc ccacgcgctc tcgatgaagc gagtcgaatc gatccgtttc gattacgcca
    46621 ttctctccaa tatcacccaa gaccatttgg actttcacaa aactatggga gactacgcca
    46681 atgcaaaata ccatcttttc gagttgctca aggccgatgg aaaagcgatt ctcaacctag
    46741 acgacgagtg gtctaccaaa ttcgcgtccc ggctgcccct cgataaaacc attctctcct
    46801 atgggctcta caatgaagaa aagatgccgg attacgccgc gaaaaaccca aaagtcacca
    46861 tcgaggggct ctcttttgag ctttgggtaa aaagggtgaa agtcggaacg gtgagaagcc
    46921 aattgatcgc agagtacaac gttcacaatt tgctgtccgt aatcgcgttg cttcaccgcg
    46981 aggggttatc tttcgacgaa atctccgcgt acatttcgga gttcaggggc gtcgaggggc
    47041 gcttcgaact ctatccgcac gtcggtaacg acatcgatct cgtgatcgat ttcgcgcata
    47101 ctcccgacgc gctcgaaaag accttgggta ccgcgcgcgc tttaacgaga aatcgcctga
    47161 tcgtagtctt cggtgcggga ggcgacgctg accaatccaa aaggccaatt atgggccaag
    47221 tggttgcgga acgcggggac gtgctcatta tcaccaccga caatcctaaa tccgaaaaac
    47281 ccttcgacat catccgtcaa atagaatcgg gcatacgcac cgacgtgccg tacctactgg
    47341 tcgaagaccg aaaaatggcg atagaaacgg cgatcaacat cgccagcaaa ggagatatga
    47401 tcattttggc ggggaaaggc catgaacact atcaaatatt cgatcacgcc tatctgccct
    47461 tcagtgaccg ggagatcgcc tttcaaatga ttcggacctt gaagaaaagc gcttcttagc
    47521 ggtctccgaa agaatcggcc gactatgatt ttcactgaat ggatagaatt gttgagcgcg
    47581 cccggccttc ggaacaaggc gatcctagaa caactattca acccctcgga agaaaccgcg
    47641 cgaggaattg aactacgatt ctgtcacgac tcacgggagg cgcgcgcggg agacatctat
    47701 atcgccttcc ccggagagcg cgtggatggt cacgactacg tcgacgaggc cttcctagca
    47761 ggcgctcgct tagccatcgt cagcgaaatc cggccatata acggccattg cctgtatgtg
    47821 gaggatacgc gccgattcgt ccaagaactc gcggctcgct ggtggaaaac cctcaaccct
    47881 aaaggcatcg ggatcaccgg gtccaacggc aaaacgacga cgaaagagtg ggtaaaggac
    47941 atccttaacc aatttctccc tcccgagacg gtctttttta atcccgggaa tcagaacacc
    48001 gaaatagggt tgcccttatg cattctgaac ggattgaacc gtcgacatcg atgggctgtc
    48061 ctcgaaatgg gccttgccaa acctggtgat ctgtcaacgc tgacagcgat gttcccattg
    48121 gtttgcagcg ttttgttgaa cgtcggaagc gctcatatag ggaatttcga agacttcgaa
    48181 gggcttttcc gtgagaaatc cgagattata cgaacctcag ctcctggttc ctctatcgtt
    48241 ctatacggga acgctccgag tatccgcgcg ttagggagcg cgtgcccgga taggcaggtc
    48301 acctatttcg gggagagtct tccggacgcc gatggcgatc gctccgtcag tttagagtct
    48361 taccgtatcg atcttcgcgg tgagagaccg gtcacggagc taaaacttaa ggtcctaacc
    48421 cggacgatta cgctctcatt tcccgtcttt ttacatcgcg ggttcgttat ggacctgtgc
    48481 gcggcccttg ccgtttgctc caccatcatc gataccgacc gattgatcga tttctcgccg
    48541 gatttgttga cacttccaaa agaccgtttc gagatacgtt cgctgcgagg aaattggatg
    48601 attttcgatc attacaacgc ttcctacgag tcgctgtgca ccgctttgga tctactggtc
    48661 gagatgaaac ggataagatt catcccccac ttccatctcg tgttgggatc ggtcttggaa
    48721 actggccggc attcgaatga aatccacacg cgtatcgcag aaaagatctt gtcgagcgat
    48781 ccgctttccg tttatttatt tagccgcgaa tcttctatcc tcgagacgca acgcgcgatg
    48841 gaaagagccc gtgatggtcg acaagccgct tctcgtatcc ctatcacctg tttctcgtca
    48901 tcggatccgg aggcaatcgc caaggggatt gcccacgact tgtctgtcag ccacaatcat
    48961 atcttctact tcaaagcttc acgaatggtg agaatggagg aagtggtaga gagtgtcatg
    49021 gatcttcttg atcgagggaa tcatcacaat tctgatctac aaatacagca ttcctctata
    49081 taagcgctgg ggcatcgttc aatacgttag agaagagggc ccacggcttc acaattacaa
    49141 gcaggggacg ccaaccgccg gaggcgtcgt cttcttgctc gttttcatcg ctttctacac
    49201 tctatcccaa tggtttagtg cgagctcctt ttactatcca gtcctcctca cggcagttct
    49261 tttcgggttg atcggtttca ccgacgactt tctgtgtgtc agaagaaaga acgccaaagg
    49321 tctgcccgcc agagtgaagc tgatagcgca attggccatt tcgctgatcg tctatttttt
    49381 ctctcaccac gggattcgat cgacgacgat ctcgctcgta cccatcagcc gaacgatcga
    49441 tctgggatgg ttctaccccg tgttcttcgt cctctacctc accgccttct caaattccgc
    49501 caatctcacg gatggattgg atggcctttc cggcggaaat gccttgattt ctgcgttggg
    49561 cgcacaaatc ttcctgttct tggtcggcgt taaagactat tcgatgattt ttttagccgg
    49621 cgctttgatc gggtttttat ggttcaacgt gaaacccgct tcgatattta tgggagacgc
    49681 cggctcgctc tccatcggcg cactgctcgg aatcatcccg ctgttaaccg gatatagcct
    49741 gttcttcgtg ttcttgtgtt tgatctattc tctcgaactc ttaagcgtag tgctccaggt
    49801 cggctctttc aagctcttcc atcgaaaaat attcctcatg agtcctatac accatcactt
    49861 cgaactcctt ggatggaagg aaagcaccat cgtgctgcgt ttctgggcct tgaacttcct
    49921 tggagtcatc ctcgccctgt cttttgttat aatatagccg acctgatcga tcacccggat
    49981 aggcagaccc ctttttcgtc gatacgggtc aatacgatac tagcaggtag agtctatgat
    50041 tttgaaagcc gaaacgctaa ccttcggatt tggagatgga cgtttgtttg agaacgtcgc
    50101 ttttcagata gacgaaggag accgcatcgc cctcttgggc gcgaacggct ccggcaaaac
    50161 ctcgttgctc aagttgatcg taggagaata cacccccgtc gaggggcgtg tcgttttgcg
    50221 gaacgggttg tctatcggat accaaacgca attccgaatc accgaccccc gacgaaccct
    50281 atggaaagaa tgcgaaagcc tattctcgag ccttaaggag acgttacgcg aatcgacgga
    50341 gattacccac gatatgctct ctttcgaaaa acgtattcgc tctgttctca aaggccttgg
    50401 atttgaggaa ccggactggg aaagagaagt gtccacattc agcggcggcg aacagacccg
    50461 catttcttta tgcaagctgt ttcttctaga ctacgatttg cttcttctgg acgagccgac
    50521 aaatcatttg gatttggctt ccgtgatctg gcttcaaaac ttcctcaaat cctatcaggg
    50581 caccttattg atggtcagcc acgaccgcgc gatcgtggaa gccgtcgcca atcgcatatt
    50641 cgagattaac gcccgaaaaa tctgggtctt taacaccgca tacgatcgct atttgcttca
    50701 gcgagatcgt atgaacgata ccctccgaaa acgcaaagac aatatagtca aagagatcga
    50761 aaggcaagaa gcgcttatcg agcagtttag gcgctggaac cgagagaaag ccttcaagtt
    50821 gatgcacgcg cgagaaaaag tggtggatcg gttgaaagac caattgaaag agtacgaagg
    50881 gatggaagag acgagcgccg aaatcggaag gctaccggta ccggaccgga ccgaatggat
    50941 cgtgattcaa ggaaaaggga tcgaaaaatc cttcgaggaa aagagggtgc tgcatcacat
    51001 cgatttcacg atcaatcgtg gccagaagat cgtgcttgtg ggaaagaacg gcattgggaa
    51061 aaccaccttt ttaaaaatac tcgcaggagt cgaccctcac cacggaggcg tcatcgaaaa
    51121 aggggcgaag acccgaatcg gttatctcac ccaaacgctg gatttccgtg acgagtcccg
    51181 agacgtcttc tctgagttgc gagagatcgt ccccgcgtgg aaagactacg agatacgaaa
    51241 gtacgccggt cggttcggct ttatcggaga agacgtattc aaaaccataa gcgcattgag
    51301 cggcggagaa aaactgaagc tcgccttggc gaagatcatc ctagaaaaac cgaacttcat
    51361 catcctagac gagcccacaa accatctgga tatcgactcg atcgccaaac tccagagcgt
    51421 tctctcggag tatgaaggat cgatgctcgt cgtcacgcac gatcagtggc tgatccaaaa
    51481 caccttcgag acggtgtggt tgttaaccga aaacgccatc acctatcccc gttccgtaga
    51541 ggactatttc gagtcggtac gcgaaagccc ggcgaccttt cgaaaaaaaa cggagcctga
    51601 aaaacggaag gtcgaagagt atcaggctca aaaaaccctg aaaaaccgtt tgcgcagcat
    51661 cgagactcag ctgaaggaaa tagagaggga ctatcgagat ttggagtgtg aaacccttgc
    51721 catacgccgg gatatgaacc tgtacgccca cgagtaccaa aaactcgaaa ccttgctaca
    51781 aaaagaaaag caaatcgcaa gcgatatgga aagcttgata cataaactcg acgccttgga
    51841 aaccgaacac cagaagacgt tgaacgaaat ggagggactc tcgtgctaac gattcgcggt
    51901 tccgggctca ccctaagcga ttacatccgg gtggccgtag agggagaaac cgtaacgctc
    51961 gacccttctt gcgtttccct gatagaacaa agcgcgcaag cggtcgaaaa catcgttcga
    52021 aaggagaata cggtatacgg gatcaacacc gggtttggcg cgctcgcgtc cgtaaaaatc
    52081 tctcctgaaa aaacgaaaga acttcagaga aatatcattc tgagccacgc ggcgggagtc
    52141 ggggaacccg ctcccaaaga agtcgtcaga ggaatgatgc tctaccgggc caactccctg
    52201 gcgaaagggt tttccggggt ccgcttgcaa accgtagaac aacttctcaa gatgttgaac
    52261 gcgcacatta caccgatcgt tccgggacag ggctccgtag gagcgagcgg agacctcgcg
    52321 cctttggccc acgtcgctat ggcgatgtta ggcgaaggag acgtttggtt tcaaggaaag
    52381 cgggtcaaag cgatcgcggc tatgcaagac gccggtattc cctccttaga accgatacaa
    52441 aaagagggat tggcgttgtt gaacgggacc caatacatcg cctctcttct cagttacggg
    52501 ttattcaagg cctttcgact cttcgattgg gcgcttttaa ccgcgagcgc cagtatcgac
    52561 gcgctgaagg gttcgacagt gccttttgat tcgcgtatcc aggaggtgcg caaccatccg
    52621 ggccaggaat tcgtcgcgca aaggatccgc ggctacttga gggatagcga aatcaggaaa
    52681 tcccatgaac gttgcgacaa agtccaagac gcctatagct tacgagcgat tccacaggtt
    52741 ttaggagcgg tttacgacgc cctcctctat gcgaagggcg ttctggaaag ggagatcaac
    52801 tcagccaccg acaaccccct cgtgttcgga gaggaggtcc tttcgggagg taattttcat
    52861 ggggaaccgg tggccttcgt cggtgacttc tgctcgatcg ccttatgcga gttgggaaat
    52921 atatcggaac ggcgcgtcga ccggatggtc aatccgcttg tctccaacct gccgcccttc
    52981 ttggcgcgcg gagaaagcgg cctgaattcg ggattcatgc tttggcagta cacggcggcg
    53041 gctctagtat cagagaacaa aaccttgtgt tttccggctt ccgtcgattc catccccacc
    53101 tccgcctatc aggaggatca cgtgtcgatg ggccctatcg ccgcccgtaa actcttgtcc
    53161 atcatagaaa acgacaccaa aatcgttgct atcgaactga tgatcgcgtt gcgcgctttg
    53221 gagttcttgc aaccgttacg atccggtccc gcgttgatga aagcgctgga gcccgtttac
    53281 gccctgctct ctccatacca aaacgaccgg tttttcggag acgatttcga acggatctat
    53341 cggtatatct ctcataatgc ggcggcaact ttttttgaaa cggccatatc gtgaatcctt
    53401 gtttccgata cctcccaccg aggtagcgga gagtgatatc cacaaagtcg atgtgagcct
    53461 acatagaacg aggagggtta tatgagattt tccgtattga aacaaaatct cgaatacgtc
    53521 gcttcgaaaa tggccgcgat cgtgtctgtc aaagcgatta aaccaatttt gttcggcgcg
    53581 ttaatcgagg tcgaggatgg gaaaataaag gtgagcgcga ccgatatgga aacgtccgtt
    53641 aaaatgagtc tcggcgagag ttttctggaa ggcgaaggcg cattcgtcgt cgatgcaaaa
    53701 ttgttggagg aagtggcgag gaacaccctt tcagaccaag ttcacttcac gttcgaagag
    53761 aatcgcctca cctatcgcgc gcaaaattcc aactatgacc ttccatttat ggagccggat
    53821 gactttcctc ctatccactt cattgaagag ggtcagagcc tttcctttcc cagaacagtt
    53881 ttaatcgaaa tgatcgagga cgtcctctat acctgcgcca cggacgaaat gatgaaaagt
    53941 ctgtactgca tcttgtggga atccgagggg aaattgcttc gcttggtggg agcggacggc
    54001 ttccgccttt ccttgagcga gcaagacatt ttcaatactg aagctcttcc cgagttcgag
    54061 gatttccgtt tcttggtctc attaaaggcg atgaaagaac tgttaaacta tctgaaagag
    54121 attaaagacg acgtggtcac actggtttac gatggcaaac gcctttgttt gggaaatgaa
    54181 tctcaccggc ttttggtgcg attgatggac tatgattatc ccgattaccg tcgtgtgatt
    54241 cctaaaactg cgaaaacgaa ggtcgtcctc gatcgagaag agtttttgaa aaaactgcgg
    54301 ttcgtatatg tcactacacg aacgagcgga gaaagcgttc gtttcatttt cacagacaac
    54361 gagctacaga tgctggctcg agcgtccgat cgcggagagg cggacgtaga gatggcggta
    54421 cagaaagagg gaaacgacct ccagatcgcc ttcaatccga aattcgtgat cgaatcggtc
    54481 cagcacgcca ggcaaccgaa gttggagctg agcttcctag acgaatcgag tccgatgcaa
    54541 atcaacgaaa cggatatgcc acggacaatc aacatcatca tgcctatgag gatgatctag
    54601 gtgtgagaag ggtcggctta ggatacgata tccaccgatt ggcgacgggg agaaggctcg
    54661 tgttaggcgg cgtggacatc ccttttgaaa aggggttgac aggccactca gacgcggacg
    54721 tgctggttca tgcgattatg gatgcgcttc ttggcgcggc gggcctcttt gatatcggcc
    54781 attacttttc cccttccgat tcccgttggg ccggtatctc ttctctagtg ctgctggaga
    54841 aagtcaaggg gatggtttgt ggagaacggt tcttccgtat agaaaacatc gacagtgtca
    54901 tcgtggccga aaatccccgc ttaagtccat atatcgacca gatgaggaag aacctctcgg
    54961 aggtgttaaa aatcccctgt tcgtcgatta tggtgaaggc gaccacaaac gaaggcttgg
    55021 gcccggaagg gttaggcgaa ggaatcagcg cccacgccgt cgttctgctg gaaagcgccg
    55081 tcgcaagccc gaaaagtcga tgagaagaaa ggatgaccga cgatggcgag gatcgtggtg
    55141 gggcggtaca taccgggtga ttctatccta caccgaacac acccggccgt gaaactggtc
    55201 tctttgatgg tttttgtgcc gtccatcttt tgggtagggg ccgtgtggct gcaaatagca
    55261 accctcctct tcatcttgtt cggaatcggg ttaagcggga tttcgccgat ctattttttt
    55321 cgcgtctttc ggttcctttg gttttttttc acgctcatct gggtattcca aatgctccaa
    55381 gccgaagggt atatcctgat gagaatcgga ccctttcgac ttacggacct gtcggtgact
    55441 cggtcgatcg tgttgtgtat gaagatcctg aacgggttct tatcctccgt catcttctct
    55501 gcgacggtat ccccgataca gttatcagat tctatcgagt ccttgttgcg cctctggaag
    55561 gtgaaaccgg aaacggctca ggatttttcg atggtattca acctatctct gaagtttttt
    55621 ccgatcctct ttgacgaggc agaccggttg ataaaagccc aaaaaagcaa aggggcatcg
    55681 ctcgattacg gtggtgttcc ttcgcgtttg aaggcggcgc aaagggtcgt cttgccgctg
    55741 cttttcaaca cgatcaaacg ggcggatgac ctctctatcg ctctggaagc gagagggtat
    55801 acctcagggg aaaaacgcgt caaataccgg cgccaacgag tcggttggaa agactggtgc
    55861 tttttaacag tggtcatagg tttttcggct ttgatgatct cctttagcgg ctagggggct
    55921 tatggattca ttttttgatc atcaattttt gtctgaaaca cgaaaaatcg tcgcgataga
    55981 tgaagccggg agaggccctt tggcgggacc cgtgtgctct tgctgtgtat tcttagagaa
    56041 ggaagaggag gacctcctcc tttcggagct tcccttcctg gacgactcta agaaaacctc
    56101 tcgggaaaaa cgtaaacgga tcgtccggtg gacgctcgac caccgaatac cgttcgcaat
    56161 cggcatggct gacgtagaag agatagattg cgaaaacatc ctccgcgcga ccgaaaaatc
    56221 gatggaacga gcggtagcga agctcgggct acccttttcg ctggcgctgg ttgatggagc
    56281 ccatttacgg ttgaactttc cccaccgcct cgttccgaaa ggcgacgccg tctccgttcg
    56341 catcgccttg gcgtcgaacc ttgcgaaaca ctatcgggac agtctgatgg aaagctacga
    56401 tacacaatat ccgcggtatg gcttcgccga gcacaaagga tacggaacgc tcaaacatcg
    56461 caaagcgata cgcgaatacg gtgcctctcc gtttcatcgg ttgaccttcg cccctctgtg
    56521 tagagaaacg gatttggcca ccctatcggc gtgggtagcc ggcggagaga tttcgtctca
    56581 taggtatgag atcatcctta aaaaaagggg ctgaaagaca atgcgaatgg tcgacttgat
    56641 acaaaaaaaa cgagaaggag gcgaactctc gcaaaacgag atagagtatc tcatcggtga
    56701 gttcgttcgt ggccatatcc ccgattacca aatggccgcg ttcttgatgg ccgtctactt
    56761 tcagcatatg accgaagcgg agaccttctt tctcactcga gcgatggaat actccggcca
    56821 ggtgatggat ctatcgacga tcaaagggat taaggtggat aaacactcca ccggcggtgt
    56881 aggtgacaaa acgacggtta tcctggcccc catcgtcgcc gcttgcgggg taaaagtcgc
    56941 gaagctctcc ggtcgtggac tgggccatac cggcggaacc atagacaaac tggaagcaat
    57001 tccgggtttt tgcacctcgc tagacttcga tcaatgggtg aaacaagtca atgagatagg
    57061 gatcgcggtc gcaggaggta atgaacagct ggttccggcg gataagaaga tctacgcgct
    57121 ccgagatgtc acggcaaccg tggaagaggt ctcgctaatc gcgtccagca ttatgagcaa
    57181 aaaattggcc tcaggggctg atgcgttcgt tctcgacgtc aaggtaggat ccggcgcgtt
    57241 tatgaaaacc ttaccggagg cgcggacttt ggccgcattg atggtgaaga tcgctgcccg
    57301 ttcagggaaa aaggcgtgcg ctttcttgac caatatggat atgcctctcg gaaagagcgt
    57361 gggtaacgcc ctagagatca tcgaatgcgt cgaaacccta aaaggcgaag gacctcgaga
    57421 cctgacggat atctgtcttt cactggcgat aaaaatggta gaatatggtg cggctatctc
    57481 cgaacaggaa gcgaaaaatc gggtattgga acgtttcgaa aacctagacg cgttgaacaa
    57541 gttcgccgaa ttcgttcgtg cgcaaggagg agacaccgcc tttatcgaag aacctggcct
    57601 gttgctcgcc gacgtcaagg tcctgccttg gtttgccagg gcggacggat ggatcgggcg
    57661 aatggagact gaagagatcg gaaaggccgc gatggtcttg ggcgccggta gagaaacaaa
    57721 agaaagcgtt atcgaccccg ccgtcggttt ggttttggag aaaaaaaccg gagacgcggt
    57781 ggaaagaaac gaaatcctgg ggtacttgta ctaccgggaa gaaaaacgcg cggaagaagc
    57841 gctcgagatt ttgaatcgcg cgattgacta cgatacggaa ccggtggagg cgccaagatt
    57901 gatcttggat atcgtcgcat gaccgttggc tgaacggtct gtgttatttt ctctttatag
    57961 gtggggggtt gcttatgagg tacctattcg ttttgttgct ggtttgcgtg ggatgtatcg
    58021 gtccagcgtt ttctcaagag ttgttctacg tgaaacaaca cggagattat gtgcgtatca
    58081 aagagccgga cgtcatagaa atgagcggca agctgttgat cagcgtgacc gccctgcgac
    58141 tattcggggc aggcgtcacc aatttcggag agtcatcgaa gtactacatc gtcgacccca
    58201 tagagcaaaa aacattgatc gcggacccgc tgaaggggat cactctggac ttctccgaaa
    58261 aagttgacgg attcgtgatc gtcatcaaca ggatgaccta catcaacgca gacctcgcct
    58321 ttcatttttt ggggttcgag tttgagcacg aggaacggta ttatgtctac aaacctcaac
    58381 atcggttgac gctcagggaa gccagaatcg atggaagaac cgtgaaattt gagttcgctg
    58441 aatccttttt taacgagcag gacgtgatca aggtccaaac caaaggaagc gccgccgaga
    58501 tcacgctctt cccggtcgac gcctctcagg ggattatcca agacttgact ctgaaagaga
    58561 gcgatacctt tttttctctc ttccaagtag agtttaacgt gccggtggcc tatcggatcg
    58621 atcgcgcagg tagcagcgtg gcggtgtatt tttctcacgt cgacccgtac ttagaagaga
    58681 tcgaaacggt ggcaaacggt atacagtggt accgaaagaa agaaactttg gcgggataca
    58741 ccctacgcgt gtcctattta gagatcctat tgaaagtggc cgagatcgaa atctcaccgg
    58801 agctagccct aggtggcata ggctataaag aaaaggtgag cgcgatggcg aaaaggaaca
    58861 cggcctacgc tgggatcaac gggtgttact tcgacaccgt cacgaatttc ccgatcggta
    58921 tactcattaa ggacgggaag ctgttaagcg agccttatta ctatccgcga ccctattttg
    58981 tgcaaacgag cgacgggaag tacgccatcc ttaatctcga ccaagaaata caactgagtt
    59041 taggggcctc cttattccta gtgaaagggg tcaacaagtt cagtaaaaac ggcgacgtca
    59101 tcatctacac ggaggagttc agccaacaga taccacgaca gggggaccga gaatacgtcg
    59161 tcgtggagaa caaccgcgtg atttctaaag agtatgccat tcgggcaccg aggaacgggt
    59221 acgtcgtgat gctctctcct atcggggtgt caaaatatgt taaaataggg gattcggcgg
    59281 aaatacgaac gatcgtttcc ggttacccgt atgagataca atttgcgata gagggcggcc
    59341 cacagatctt ggaaggcgga aagctgatct ctagcgtcga tacagagaaa gtgagatacg
    59401 gcgaacggct gtt
//
LOCUS       DAOPXN010000002        35925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_1120_length_35925_cov_5.342292, whole genome shotgun sequence.
ACCESSION   DAOPXN010000002 DAOPXN010000000
VERSION     DAOPXN010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 35925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35925
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1120_length_35925_cov_5.342292"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            769..1467
                     /gene="thyX"
                     /locus_tag="PKM49_00260"
     CDS             769..1467
                     /gene="thyX"
                     /locus_tag="PKM49_00260"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNY81276.1"
                     /translation="MVHLVLDKGYVQEVDLLGNDSRAVEAARVSYQQGLKSVDRDRSL
                     IDFLVENGHESPFEHIVFTFRIKAPLFVARQWFRHRIGSFNEISARYSEMSEEFYIPQ
                     TSRSQDAVNRQASVVEDAPEKDRLLREVVAEHSRQTYERYRELLSSGVARELARIVLP
                     LNLYTLWYWTVNARSLMNFLSLRADEHAQWEIQQYALAVGAIFKERCPWTFTAYMKYK
                     YAGRLLELLQRECD"
     gene            1469..1822
                     /locus_tag="PKM49_00265"
     CDS             1469..1822
                     /locus_tag="PKM49_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HNY81277.1"
                     /translation="MKLIDISKILEAKVYSGEEKLNDLEIDRAGAADMMSDVLAFAKP
                     GMLLITGLNTPQVVRTASIVGIATVVIVRKKQIPAETIQAAKMFGIPLLHSPLPMYTA
                     CGVLYAHNLKDIRAF"
     gene            1838..2794
                     /locus_tag="PKM49_00270"
     CDS             1838..2794
                     /locus_tag="PKM49_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNY81278.1"
                     /translation="MKPTVDKLIDQMQMLFKDLAAKDLMVPNVISISPDRTMEQAKEL
                     MRLKKISGLPVVENDILVGLVSIEDIIKALERHRIHDPIYKIMTKNLVVVSPDETLPQ
                     IFDKFTRTGFGRFPVVDDQKRLVGIITKEDILRGILEEFRLLYVRDKRRQDVIDGEAL
                     NRSLITGEEINKLGADFAFSIDYVEVSLSGVGAARLKDFLKGRGIEEEIARKVAIASY
                     EAETNVVIHSGSSGNIYCFLYPEYIRVRVEDHGKGIEDLDKAMQEGYSTATDQIREMG
                     FGAGMGISNMHRYSDKMVIVSEVGKGVIVEMQFFLKDAANSA"
     gene            2807..4135
                     /locus_tag="PKM49_00275"
     CDS             2807..4135
                     /locus_tag="PKM49_00275"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HNY81279.1"
                     /translation="MPKGFPKEFFWGTATAAYQVEGADAEDGKGPSIWTSFSRQPGRI
                     ADGHTGDIACDHYHRFEEDLALMKELGVNAYRFSISWPRVLPEGRGPVNTPGMDFYQR
                     WVDRLLENQITPFVTLYHGDLPLALQERIGGWAGEDIDRYFGDYAALMFRTLGDRVTY
                     WITLNEPMRFSFIAYGKGEYTPGEQNLKTAFLVAHHLLRAHAQATARLRELLPNGKVG
                     LINAASWIEAASESGEDIQTAFIANQLLHDWFFLPVLTGKYPEEAAQFVRGLGLLPVF
                     DPQEMSRIAKAPDFWGINYYTRRRVKMDMSRPIFFAYAPPERETTESGLEIYPQGLTA
                     FLKIAQKEYGSIPIFVTANGMADKDVLSNGVVADERRIAYLKSHIEAVQRAIAEGVDV
                     RGYFVWSLLDGFEWTQGYDKRYGLIYVDYEKSLTRIKKDSYYYYKNYLAL"
     gene            4160..4699
                     /locus_tag="PKM49_00280"
     CDS             4160..4699
                     /locus_tag="PKM49_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81280.1"
                     /translation="MKKTLLVFILIGMLVFLTGCQFEPEETYRVSNLIEMRILPYTRT
                     ELKITTPATVLQKKDTKDGALVLYGTIFEAVYTTPNAKEIKLTVGRTDTLRIADELWR
                     RYRKEDAPPFWTWVNYLPFFYGSYETQSATKFAMVWFSKKWVFRIVGPDEQTVRDFYR
                     KLTSFIEKKEVFNGSIREI"
     gene            4825..5823
                     /gene="murB"
                     /locus_tag="PKM49_00285"
     CDS             4825..5823
                     /gene="murB"
                     /locus_tag="PKM49_00285"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNY81281.1"
                     /translation="MTHQKAMNQGDRDANDENGDLARQEDSSRLMIDRRFVERLYRLE
                     TTVRVNEALSKHTTFGIGGRVPLYVQPLERRALPEIVPLLKESGRECKILGIGSNVLP
                     DDGPLSFIVLDTTGLIGYSQESEGFRVEAGYPLRQLSRLFYARGWSGLEFSAGIPGTV
                     GGGVLMNAGAYGSQIGDLVLEVEAFDWDSECLRVYPQGECAFSYRSSRFSQSGELITR
                     VLLKTPPGERAEIGDRMREFERQRINKQPLEQGSAGSIFKKPAADFHVGKVIEELGLK
                     GRRYGGARVSSLHGGFIVNEGSATSADVRALIDLIRTRVHQAYGIELELEIRLWSH"
     gene            complement(5944..6678)
                     /locus_tag="PKM49_00290"
     CDS             complement(5944..6678)
                     /locus_tag="PKM49_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81282.1"
                     /translation="MQEVTQTVSVTDIYGFFQLLITYWYIALPFSIALLFASRWFPKV
                     LIAALGFVGGLFFLMPLLKTIPAVNELLVSNPSLDLPIALFVGVICAVVVLGLFKFVF
                     FLGGVLIGFFIGVWLWNIANPWIIDQIRLQTPSFEIPQWTVWVFAGVFALIVGIFAIL
                     SHEKTVSFLSILSGALILDFYLLFLLGQSNPGLFGMITKPSGANEAPALTTNGILVFV
                     GALLVLMFIGYRFSYSHWGARKTKNP"
     gene            complement(6717..7547)
                     /gene="amrB"
                     /locus_tag="PKM49_00295"
     CDS             complement(6717..7547)
                     /gene="amrB"
                     /locus_tag="PKM49_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNY81283.1"
                     /translation="MRRKPVVSGVFYQDNLIALRNQIEKLFLGPIGPGQLPDFPTKPL
                     QKSLGAVLPHAGYVYSGSVSAWGIWELAKRGKPETIVLIGPSHTGMGKPISIWNKGSW
                     ETPFGQVEVDEKLADLLLSRYNYASGDFEGHMGEHSLEVNLPMLQYIYGKDFRILPVS
                     MMDQRKQTALDLGNQFSFLKTLKPLLIIASTDLNHYESESVTRSKDQEVIDAILSMNV
                     ERLYSVISEQSITMCGFGPAATLMSSGEWKPKLLKHATSGEITGDYQRVVGYVSMLFE
                     "
     gene            7885..9243
                     /locus_tag="PKM49_00300"
     CDS             7885..9243
                     /locus_tag="PKM49_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81284.1"
                     /translation="MRRAVVSCFLLFCYVSTLFGLEASLSFYLLSKHDKVVRNFGLAG
                     GFHTEVYRDDLQIVLDWQLRNFQAGDFKINFPLLAGAFKSDKPIYYYPQESFLGYDNG
                     TSQFKIGYFPLNEGAGHHYPLWISDNSGAYPAIRFEWNPEHWFTFTNAILFMRQGVQD
                     WKSENNLQTAKTLYYRKAGFRPFYCWEFGFEESMLFLGRTLDLPYLISFFPYQSMQEL
                     RNNFDAPWKEKFNDNGMIGFYTKFYLDPVDVYASVFIDDFNPTNLKAANNKLAWNVGL
                     KWLITDRISLSLESAGASKYTYQRHSLSVPPYMYVRYETEATLPIEYNMIGYRYGPNS
                     GVFNVEAAYRADRWGGSVNYEFLVFGKRAPLEQWDEHGGTMPVLTGLPWLNDPVLQYE
                     NTIGLNLYGDVLENLRLNGSLGITFVKNDGLEKGKSDTRFSFAFGAQVKIPLTALIDR
                     LW"
     gene            9347..9796
                     /locus_tag="PKM49_00305"
     CDS             9347..9796
                     /locus_tag="PKM49_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNY81285.1"
                     /translation="MDKLDLLNIALRIESTGYEGYAQLAEKTKGKMKEFFSHLAAQER
                     EHFETFKTIFQKIEKHEEPQSWGDLEALGYWQAYAQVAIFPKISNPKVPATMQEAIHA
                     AVEAEKESIIFYNGIKEYFEDKTEIQKIIQEEQKHLLDLLSLPDARK"
     gene            complement(9908..10270)
                     /locus_tag="PKM49_00310"
     CDS             complement(9908..10270)
                     /locus_tag="PKM49_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNY81286.1"
                     /translation="MTLYERIYEVIRHIPRGRVATYGQIARILGVCGPRAVGYALNSS
                     KDPTLPWHRVVNSKGRSSLEQEGAQLQLELLKLEGVTVDQRGAIDLSLFLWEGPTYEW
                     MRGRLVGEGTSGRASEDR"
     gene            complement(10305..11405)
                     /gene="wecB"
                     /locus_tag="PKM49_00315"
     CDS             complement(10305..11405)
                     /gene="wecB"
                     /locus_tag="PKM49_00315"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNY81287.1"
                     /translation="MRIGLIFGTRPEAIKMAPVYLELVSRGHQASIIATAQHRQMLDQ
                     VLEIFGLEPHIDLNVMTERQSLSSLTSRLCQSLGEAFERQPLDVVLVQGDTTSTFVGA
                     LAAFYHKIPVGHVEAGLRTANRYDPFPEEINRRLTTQLSSWHFAPTAHAKESLLREGI
                     DPSKVFITGNTVIDALLWVLRHKREATERILRTYALEKGSYLLVTMHRRENWGERMEA
                     VCGALLRILNDRPELKIIFPVHLNPRVREVVFPLLQDRPRVILCDPVGYLDFVGLMAG
                     SKIILSDSGGIQEEAPTLGKPVLVLRETTERPEAVEAGTALLTGTDPKRVYDTVMALL
                     KDTERYDRMSRAANPYGSGEASKNIADIILTG"
     gene            complement(11518..12855)
                     /locus_tag="PKM49_00320"
     CDS             complement(11518..12855)
                     /locus_tag="PKM49_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNY81288.1"
                     /translation="MRILLINPWIADVAAYDFWLKPVGLLILGGYLSRSGHELHLIDC
                     LDRYDPQLARATGKLPRSKANGTGKFLCREIEKPRAIKWLPRRYKLYGWPEELVARRL
                     RELKERWDPEIVMVTSAMTYWYPAVWKMIRLVKETLPRARVLLGGNYPVLLPDHAALS
                     GADAICIRSDWVGVRVFLEAQGVSTIEGPDSLIPLYDLYPGAMSHLVFLTSVGCPFHC
                     SYCATGLIHRFFQHPFEELAVAIEAACQSHKCQNVAFFDDALLVDHTSHFDLLLKMLI
                     QRGVPTSGISFHLPNGIHARLLTKETARLMRQANVQTIKVGLETLDLALQAQTGSKVT
                     TEEFVRSIEILRSVGFSKTEVSAYLMVNLPSQSIEDVEAAVRLCERLDIGFNLNEYTP
                     IPRTAQYEDLVQSGKLPREVDPVLLNNTILPYWWPHGVSADEIAALKKRVRVG"
     gene            13005..13304
                     /locus_tag="PKM49_00325"
     CDS             13005..13304
                     /locus_tag="PKM49_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNY81289.1"
                     /translation="MFVLGNFLLALAQIIQLILYAYIFILILNSILSFMGNSYQLYQI
                     KKVVSGLAGLMLNPLRKVFKPIGNVDYTPFIAILILVFIQSFVVQTLIDIGSRLR"
     gene            13301..14128
                     /locus_tag="PKM49_00330"
     CDS             13301..14128
                     /locus_tag="PKM49_00330"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNY81290.1"
                     /translation="MSVAVGILFNPHKPAIEKYLDILIERLARKGLKTPFALSTEGPE
                     FVSRSEFPSPIDYLLVLGGDGSVLRAMQATLQKQIPVIGVNFGRFGFLTRFEIEEVID
                     PDFNLSEITVSRRLVLGVRALKGVESLTGYALNDVVVHRPLERNIEHYHIRINGESLP
                     FIAGDGVILSTPTGSTAYSLSAGGAIIYPQAYVFQITLLAPHTMARSPIILDDGSVIQ
                     LEKNPPSSLYYSLDGFTVKTGDSLEVRRAPYDFLLASPKGESLSAIISEKFLWGRRG"
     gene            14131..15753
                     /locus_tag="PKM49_00335"
     CDS             14131..15753
                     /locus_tag="PKM49_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81291.1"
                     /translation="MLREMRVQNALLFKQAEMAFEDGFCAVTGETGAGKSMFVSLLRS
                     LSGELKGRDLLGPFGDRFSIETLWDGSEQVTRLLSENDLPQEDGLLLKVAGTEERLSY
                     RVNGSLASANVMREIANALVSIHSQNAFQNLRNPLFQTAIVDGYRPQALEEIARRYRE
                     GFRRYQELKALSGALPTDTREMARALDFLAFQIEEIEKARLVVGEDERVFAELRVLSN
                     FEFLKNNLLGALAQIEGEGDFDSIADRLGEIASKLKRVNGIETAVEGWEKLTVELSDT
                     LHQLRSEISAYLERMDYDEGRLREVSARYDLIESLKRKYGKDIPDVLARLEAFREEKA
                     ALQEKMSLAEHLDEKIEAAKHVLSSIDAEWRALREKNAAAIEASVVRELADLRMEKTR
                     LAHEISPEEDFTAHGGHTLEWTASINPGMPFLPISKIASGGELSRIFLALELALKESL
                     SVKTLVFDEVDSGVGPRMGHVVGDKIKQLAQNGFQVFVVTHLPQVAARADQHFVVRKM
                     SDEAQSLSVIVEARGTERDREIREMAGEQEAP"
     gene            complement(15796..16365)
                     /gene="fliS"
                     /locus_tag="PKM49_00340"
     CDS             complement(15796..16365)
                     /gene="fliS"
                     /locus_tag="PKM49_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HNY81292.1"
                     /translation="MQSKQLYTNLRPGFPRRSPAVQEPAASPNVVATVGEPPIQKQKP
                     APTLFHHPQNQNYFEQSIFTASPAKLVEMLYAGALSFIEQAKNAIAEKDYITANTKIV
                     RAEDIIVELNISLDLEKGQVIATNLRDLYNYLYKRLLEANSKKDTKILDEVYQFVKEL
                     KDIWGEVMKKEPSNPQDSPVDRKILDLSR"
     gene            complement(16387..16995)
                     /locus_tag="PKM49_00345"
     CDS             complement(16387..16995)
                     /locus_tag="PKM49_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81293.1"
                     /translation="MDSMIQVSVNGRVKEEDEKIYQNFGDFIAKQQVKGKVLAEVKIN
                     GHDIPLSALKELELSVFEGDEKIELVFKEFRPFTVELIGNVKNYLNHITTRLPFYAER
                     ILSGDIEALREINDLGEGLNSVLTMKENIFAISGCQETDFRSLKGLEPAFSELLNEIN
                     ESMGRKDWVRLSTALSHELPDKLKTFSAFFDEATVLLQTNSM"
     gene            complement(17002..17565)
                     /locus_tag="PKM49_00350"
     CDS             complement(17002..17565)
                     /locus_tag="PKM49_00350"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNY81294.1"
                     /translation="MNRLYEKELFREYVFKGKILRLRLDQVELENGSKARREVVEHDG
                     AVAVLPFDADGYVHLVTQYRKPIDRSLQEVPAGRLEVGEDPIACAKRELREETGLHGG
                     EVIPIGYIYTTPGFCDEKIYLFVAFDLRPGENHPDPDEFLNVERLPWEAFIERCAQGQ
                     ITDAKTLALALRARAKVEAYFSDKKSG"
     gene            complement(17589..19985)
                     /locus_tag="PKM49_00355"
     CDS             complement(17589..19985)
                     /locus_tag="PKM49_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY81295.1"
                     /translation="MFSSWKKAIFYTLLVIGAFVMLMPFAWMILTSVKLDTEVESWPP
                     KWTSNSFAKQRNVKLNLVRSADVALDFSSLSIEEFFNLASIIGSKKKGEKTLVYSVND
                     DTPYRGIFELNLNAGAGKNPTYARAIPRAEFDKFLTEQGALRPLPDEVQSILDTVGMV
                     DDPIVYLPSLMNELFFSSHAFLNRIGVVTSSDGVLNKVNAYLVSNGVKLVEHRALLPS
                     EQDEPQTALLKAAYRDWLERLVRTFETQKGIYTVANQFFRKGQPLLSQADLEEISAKY
                     AQTFADGLTYTGLAAALNVSAEEAEALVSEQDWAVIRLVDRNIREPLKSFQNLLRLGI
                     TTYRFYTRLQTDQLSSGTLRFQFRKEADIKQDILEQIKNSSLTQEHKRLTQEVLEGHP
                     VDTSVNALLKELDRIFTSHLSERGIDPSKIQSTLLNLKDFVGTLNSVFLDHPELKEPI
                     MRAFLGVGDPVDVLNASSVPASQRRSLVDQLNRLKTLFRGQPDALLYVLIHERFVENE
                     VVEYYSEVYSRYGYRMDVLEAPKEVKDVRFRSYKSIEIVLDGIQPYWFWDETQQLQVS
                     FTFKEIFQNVFQSYVDAWVMGKYFGNYYFNTVFVALVTTLLDVLFACMAAFAFSKLNF
                     FGKNFIFMVFLATMMVPGEVLLVPNYITLARFGWMDTYLALIVPWVVSVFVIFLMRQH
                     FMTIPDELYDAGKIDGISKWGFLWKVMAPLSKPVIITGALLKFVGSWNSFLWVLIVTK
                     SPQVRTLPVGLSTFSTEVGTLYNKLMAASTFSMIPVIILFLFVQKYFIQGIARTGLKG
                     "
     gene            complement(20003..21325)
                     /locus_tag="PKM49_00360"
     CDS             complement(20003..21325)
                     /locus_tag="PKM49_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81296.1"
                     /translation="MVYVGKARKRYWRETFHAYLFLLPAFIILGAFVFWPIGYSLILS
                     FFKWDFTNQRAPFFHGFTNYTQLFKLQMALPYSFWQACVYSAFYIVAAFLLARLVFLW
                     THYFKAFESHRLGRERLLRMTAIYAALTVLGLLFAHNGLIWPLFGTVVLMGSLYFFEN
                     RRPYAFKKPMGLWSLAVSVLLLYFALKRISLVRYELFDFLLLTKESSVFVKSIFNTIY
                     YVILTTPAEIVISLFIALLLNLPLKLKPFFRTAYFVPYVTSVVAISLVWRWIFNDEYG
                     LFNYFLSLIDISKVKWLTQETWTIPTIAIVSIWKSVGYNAIIFLAGLQSIDKSYYEAA
                     KVDGASNLQNFYHITWPLLSPTTFFILIVSVIGNFKVFTQVYVLYQALPGPYNNSGMT
                     MVYYIFDRFYAHQQMGEASAAAYVLFMIILVLTFFQFRVGKQRVQYVG"
     gene            complement(21414..21866)
                     /locus_tag="PKM49_00365"
     CDS             complement(21414..21866)
                     /locus_tag="PKM49_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNY81297.1"
                     /translation="MNHTPPKGKIAFVLAKGLPLDFIEELLDLVKANRFEIGRIIGVE
                     REIETVKETLFDLCMQGFSLIIVFGAIGVEVDDVVPEATASIIDKTLPGLERTIMYLL
                     LENDEYSVVNRAVAGLYKKSILFNLPAKPKLIRLVLENILGVLQKAVR"
     gene            complement(21863..23023)
                     /locus_tag="PKM49_00370"
     CDS             complement(21863..23023)
                     /locus_tag="PKM49_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81298.1"
                     /translation="MDRMFVKGVALETLIAEPGFVELFRDENSGVLLSLIDSYFDPTS
                     SFIFGTPSIHLYVRSSAEEFVAFAYLDLKSTTFSFNASETDHPIAAYIKRIFSNYVWG
                     ELNVYRYESFTLDDIIDELHFFAGLNTYVLGFAASDETLFNELDQRVSDLNMIFNARE
                     SRRIAHGISLMNMVARESSEYKDQTTHYLVPTVGVEGLEVWLGTNSTFFVHLTEMDGN
                     CDSANPTEVEERKSLLKFLFNIVFLRFCSYLSKRYEIQYYTISKIERAFILEGKDQNT
                     VRDFLSCIRTINQKAVDFFRQELLPICEDFSKILNAALKKYQQISTFYDESNFIYITI
                     LALTDETSEYQSVFESLFHQNINKLIEQLLLIITQVDRAIARYVEEGKAERI"
     gene            complement(23024..23392)
                     /gene="acpS"
                     /locus_tag="PKM49_00375"
     CDS             complement(23024..23392)
                     /gene="acpS"
                     /locus_tag="PKM49_00375"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNY81299.1"
                     /translation="MSKIGIDIVDMERLTPQMIDRIRMRVFSKSEIAEMEALKNPRRR
                     IEYFAGRFAAKEAFFKALGIGIFNTVLDQVEVLADETGRPYLRVSEAVRSAYIGEYAS
                     CSVSISHDGSYAVAVVLLEK"
     gene            complement(23412..24032)
                     /gene="nth"
                     /locus_tag="PKM49_00380"
     CDS             complement(23412..24032)
                     /gene="nth"
                     /locus_tag="PKM49_00380"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNY81300.1"
                     /translation="MDAKQVAHVCERIISFFPRIHHETDPFRVLISTIISQRNRDEVT
                     ERVSAKLFGLYPTAKHLAAQEPEALYPALQESGLYRQKSKWIVEIAKRVDEAGGVVPN
                     DLEALCRYPGVGRKTANIVLSVSFGIPALAVDTHVHRISNRIGFVHTKTPEQTEVALT
                     SLLPASLWGDLNGSMVEMGRNVCKPLKPVCSACPVFDDCEWSEKHR"
     gene            complement(24041..24913)
                     /gene="atpG"
                     /locus_tag="PKM49_00385"
     CDS             complement(24041..24913)
                     /gene="atpG"
                     /locus_tag="PKM49_00385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNY81301.1"
                     /translation="MGRGALHTIRRRIDSTESTQHITHAMEMVAAAKVNKVFRLWKGF
                     TPYLASLSAMIDECVIADPDLEHPLVEKKKPEEEKILFLIITSDMGLCGSYNLDILRM
                     AEEEEERRGDAFKGYFICGTKGIGYLRYRNKKIYGSLDRFFGTPDFSVADLLSGRILS
                     LIESGEVNTVKVISSHFKSSLIQKPAITDLLPLKPKPPQKAQWQREFEYEPERNVLLN
                     SLIPLYLSSVLLHLLLEAQVSELYSRQNAMRNATENAEHLIERFTLAFNKARQGFITQ
                     EIIEIVNGAEALKE"
     gene            complement(24927..26435)
                     /gene="atpA"
                     /locus_tag="PKM49_00390"
     CDS             complement(24927..26435)
                     /gene="atpA"
                     /locus_tag="PKM49_00390"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNY81302.1"
                     /translation="MKIKPEEITQILRDKITGFEKTAELEETGQIVQVGDGIATVYGL
                     KKVKASELVLFENGSKGLALNLDEDSVGVVVLGEYHNLKEGQLVKRTGKIVSVPVGEA
                     MLGRVVNPLGEPLDGKGPIVTQDFREIEFKAPGVIYRQPVNTPLQTGLKSIDSMIPIG
                     RGQRELIIGDRQTGKTAIALDTIINQKGKNAKCIYVAIGQKAASVARIVDQLEQFGAM
                     DYTTVVFASASDPASMLYIAPYTGCAIGEHFMYQGQDALVVYDDLTKHAASYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAARLNPSLGGGSLTALPIIETQANDLSAYIPTNV
                     ISITDGQIYLEAGLFYAGFRPAINIGLSVSRVGGTAQIKAMKKIAGTLRLDLAQYRDL
                     ESFSQFASELDEATRSQLTRGEKLMELLKQNQYSPIPVEEQVISIFAGVRGFLDTIPT
                     HRIRAFEEQLIRYVKHQHPEILSKLKEEKDLSTELTRSIEEAIKAFKKVFVG"
     gene            complement(26441..26995)
                     /locus_tag="PKM49_00395"
     CDS             complement(26441..26995)
                     /locus_tag="PKM49_00395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNY81303.1"
                     /translation="MKNPSQKVILVYAKALLTGDTALASQALRVLQETLTLDLYRSIV
                     FHPSIPKREKIAALAALLRAKGCYSEVMERFLTEVLEKRREKILLDLSPVFDRLLAES
                     QKGTEGTIFTPFPLSEEQKGFVSKELIAYFEAKGYETPIHFSFALQPDLLGGIKACVD
                     DHILDLSLQAQLLKAKRHLNAEER"
     gene            complement(26992..27489)
                     /gene="atpF"
                     /locus_tag="PKM49_00400"
     CDS             complement(26992..27489)
                     /gene="atpF"
                     /locus_tag="PKM49_00400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNY81304.1"
                     /translation="MIHLEFNLTSILMLLQFILLAFFLYKFLYKPFFKMSDERRKRIQ
                     EDFSLAEKAKLEAEASQREAKQLLDNAVAQAEGIIANAKKTAEDYAIRERIDAKQKAE
                     SLLRKAQEEIEAQKKEAIQEISEKSVSLAVYLASKLLERQVDEKAQSEYLKSFLAKIK
                     QGGGR"
     gene            complement(27585..27827)
                     /gene="atpE"
                     /locus_tag="PKM49_00405"
     CDS             complement(27585..27827)
                     /gene="atpE"
                     /locus_tag="PKM49_00405"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HNY81305.1"
                     /translation="MEMSAAIVQAAKYLGAGICMGLAAIGPAIGEGNIGAHAMDAMAR
                     QPEMAGNLRISMIIADAIAETTGLYGLLISFMILFA"
     gene            complement(27967..28812)
                     /gene="atpB"
                     /locus_tag="PKM49_00410"
     CDS             complement(27967..28812)
                     /gene="atpB"
                     /locus_tag="PKM49_00410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNY81306.1"
                     /translation="MTLSRTEKTFVIAMVIAIIVVWILALPEMGGMTDLSDAGIGTRW
                     VYTFPGSEGSFFTRINFLTVGVGLLITLFLIGVARGIRKTFSLVPNRKQSLVEYLLQF
                     FYDLTGEAINDKRYVKPVFVIAMTLFLFIAASNILSGIPGINVSIRNQETYLTFFTDT
                     WYVPTSDLNTNGTYALIVLVISHIFGIKAKGFKAWAKSFFEPIAIMFPMNVIGELAKP
                     VSHSCRLFGNIFGGGILVFILSYILKFLFLPMFFWGFFGMFFGLIQALIFSVLTIVYI
                     GSQVN"
     gene            complement(28799..29221)
                     /locus_tag="PKM49_00415"
     CDS             complement(28799..29221)
                     /locus_tag="PKM49_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81307.1"
                     /translation="MRRRIFRAFFPRFDLKAMTTEALLKTSVYGIIYLNSGLFLIGFF
                     FFSHPFGYGVLAGTAAALMKVWVYAEQTRRMEKKTQYVSASAFFLRYSIDALAMGISA
                     FFSIQTLLGCFFGTISLKWVMVWLSFALRNGGKKDDPQ"
     gene            complement(29218..29517)
                     /locus_tag="PKM49_00420"
     CDS             complement(29218..29517)
                     /locus_tag="PKM49_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNY81308.1"
                     /translation="MSEESEKKPNPFKELRHLNILYIFGITIIANTFVGGAIGYFLDK
                     YTFKNRVLLVIFLVLGIASGLYQGILGLLKEAREMESAKAEPKAENDRPKNKGAP"
     gene            complement(29514..29969)
                     /locus_tag="PKM49_00425"
     CDS             complement(29514..29969)
                     /locus_tag="PKM49_00425"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNY81309.1"
                     /translation="MDVRIINSILKSTVKIFTEAVNMKIEFEKPQIAKELAKGYDLVT
                     LVGFNGSLSGNLVYAFDTQVALSVVQKMMGMPYNQLDELAMSAIGELANMISGNIATN
                     MEQIGKPIDITPPSVILGKEIRVNVDGVILKIITKYEDKTFEVDMVLRE"
     gene            complement(29972..31507)
                     /locus_tag="PKM49_00430"
     CDS             complement(29972..31507)
                     /locus_tag="PKM49_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNY81310.1"
                     /translation="MASFSKLNSAVLVGLHARHVLVESFVDAQAVIHEIDIVGLGDTA
                     IKESKKRVKSAISSNKLNIPQGRIVINLAPGNLKKEGTLLDLPIAVAILACGNDVERE
                     IPPGLFFFGELGLNGVLRRVQGMLPLLIALSKSKEPKTIVIPQENVEEAKLVPEIDVR
                     LAADLWEVLSFLNGGELPRVPSDRALEPDLPIEDDFSEIHGQYQAKRALEIAAAGGHN
                     VLMKGPPGSGKTMLAKRLPGLLPPLREEEFLETASIYSIVGLLNSEILRRKRPFRSPH
                     HSASRAAIVGGGVDSRPGEVSLAHNGVLFLDEIPEFSRDVLEALRQPLEDRIVSVARA
                     KGTLTYPASVALVAAQNPCPCGNYGDPDLVCTCSARDIVNYNRKISGPLLDRIEIVIE
                     VPRLTYKEYTSKEGGEGSASIRERVVKARETQNRRFRKTHHRGFTNASLNSKMIQEYC
                     RLDGEAETLLEQAVNRYHLTGRGINKVLKVARTISDLEGSETIQAVHLAEAIQYREKI
                     TEG"
     gene            complement(31531..32409)
                     /gene="pdxS"
                     /locus_tag="PKM49_00435"
     CDS             complement(31531..32409)
                     /gene="pdxS"
                     /locus_tag="PKM49_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HNY81311.1"
                     /translation="MESKSTWTVKTGFADMFKNGVIMDVTDARQAKIAQDAGAVAVMA
                     LDMVPADIRKTGGVARMASIKVIKEIMNTVSIPVMAKCRIGHIAEARVLEAIGVDFID
                     ESEVLTPADDRFHVNKHEFKVPFVCGCRNLGEALRRIAEGAAMMRTKGEAGTGNIVEA
                     VKHARQVWEEVRKLQNTPDEELVTVSKETGAPIDLIRTVKKLGKLPVVNFAAGGVATP
                     ADAALMMILGMDGVFVGSGIFKSEDPTKMASAIVQAVRHWNDFNMLAKISEDLPPAMK
                     GIDISELEVHLQERGW"
     gene            complement(32414..33700)
                     /locus_tag="PKM49_00440"
     CDS             complement(32414..33700)
                     /locus_tag="PKM49_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNY81312.1"
                     /translation="MPRFDPSLFKIPIDRIRAGYYSDKYFTRYVDVLRREGVSHHALY
                     QVFPRSEACIVGIEEALAILQSCTGYYNEPDKAADIFSDILDAERKLQKALYIRDYHA
                     ADGATKEKWSLRDRLNALWVDKWDSIRVLALTDGDMAKDAEIIMTIEGDPVYFGSLET
                     VFLGVISRASSTATAVKKVVNASRGKPVVLFSARFDHYWVQATDGYAAFKAGAFGVST
                     DANADYWGSKGLGTIPHALIACYKGDTYRAAEAFDRWIEPEVNRIVLVDWDNDCIGTT
                     KEVLKRFYENTTGKTLSPATKDLSTIIGPGRHKIWGVRFDTSGSLRDRSVTPHDERSL
                     GVSPELVWKARMEFDRIGAEKLKIMVSGGFNADRINLYERLEVPVDVYGVGSSLLREK
                     VDFTADIVRMDGIPCAKYGRGQGDYTRLKEVHYKEE"
     gene            complement(33734..34933)
                     /locus_tag="PKM49_00445"
     CDS             complement(33734..34933)
                     /locus_tag="PKM49_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNY81313.1"
                     /translation="MNLSKRVFAMQASPIRRLIPFAEEAKARGTSVFFLNIGQPDIPT
                     PPVFMESIRNYKEEVVAYSHSAGLLELRKAFARYFERWQTDLKPEEIIITSGGSEAVV
                     FAVAAVADPDEEIIVIEPFYANYKGFAGMVNVRLTPVAANPEGGYRVPEDKAFERVIN
                     PKTRAILFSNPCNPTGAVYTKDEVLRVLKIAEKYDLMVIIDEVYREFVFDGLQPFSAL
                     EFPEYRNRIILIDSVSKRYSSCGARVGVFATKNKEVYAQVMKMAQARLSPPTMAQVGT
                     LGLLTLGEDYIYSVRDEYRLRRDKVYEELSRIPGLAFKKPQGAFYLSVKLPIDNSEKF
                     VKWMLSDFSHNGHTTMVSPLDGFYATPGAGEQEIRIAYVINAEKMKKACEVFRVGLEE
                     YIRLQRA"
     gene            complement(35071..35253)
                     /locus_tag="PKM49_00450"
     CDS             complement(35071..35253)
                     /locus_tag="PKM49_00450"
                     /inference="COORDINATES: protein motif:HMM:NF025436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HNY81314.1"
                     /translation="MEPISADYMALRNAELSNQVQTAVMKKTMDAALEQSEQLLATLP
                     KMPTEPGLGNILDRRV"
     gene            complement(35654..>35925)
                     /locus_tag="PKM49_00455"
     CDS             complement(35654..>35925)
                     /locus_tag="PKM49_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNY81315.1"
                     /translation="GVFHGLMETIENFRYEVIVDGKEDEEKKTFTLAGPTCDSVDMIY
                     QQIDLPANITYGDIVYFKNTGAYTVEYGTYFNGIRPPKTYILDYA"
BASE COUNT         8297 a         9426 c         9634 g         8568 t
ORIGIN      
        1 ctcccttttt tagttgtctt tcggtcaacc ggattataac acaccactca tctttgcgga
       61 catccagtgt gggtataatc tcggggtaca ccaaattggg aacgcagaac tttaattcgg
      121 catgtttttt cgcttttcag taagctctcg aggcccttaa acgcttacac tgcgatggcc
      181 ttacgaaagc gcatcgcgcc atcgcgaaaa aaaaggtgcg ctgacgccgc gatgaccgtg
      241 ggggttatcg cggggtaaat ggtttttacg cccccgtgat ccgccttttt tttacgccgc
      301 gatcagctcg catagaagcg tatcgcgccg tcgcgaaaag aaggggcgct tacgccgcga
      361 tgactatatg gccaaaaaac cgtgcgatcg attctggcct tgtttcttaa ttacaccccg
      421 tagcttgaca cacacggcgt ccagtgtgtg ttataaaaag atggaaaaga gacgaaaaaa
      481 acggaagaaa acaggatggc ctcctaagga acgatggaac caaaaccgaa acctcaagct
      541 gttcggagcg gcggccttag agaatcttgc ctttcaagaa gtgtaacttg tctagtaaaa
      601 aactcgatgc tccaaaacag agaacacaat aaccgtttct gaactttccg gctgtttttt
      661 tggtagaata gattgataag cggactttgg aatgagagca cgggttaccc tgtccgggta
      721 cgcgccgcga cgggtgccga aaggatgagc gatacgaaaa acgatcgttt ggttcacctc
      781 gttttggaca aagggtacgt tcaggaggtc gatcttctcg gaaacgacag tcgcgcggta
      841 gaagccgcgc gggtctctta ccaacaaggg ttgaaaagtg tcgatcgcga tcgttctttg
      901 atcgattttt tggtggagaa cgggcacgag agtccgtttg agcacatcgt cttcacgttt
      961 cggataaaag cgccgctctt tgtggccagg caatggtttc gccaccgaat cggcagtttt
     1021 aacgaaatca gcgctcggta cagtgaaatg tcggaggagt tctatatccc gcagaccagt
     1081 aggagtcagg atgccgtcaa ccgtcaggcg agcgtggtgg aagacgcacc agagaaagat
     1141 cgccttctga gggaggtcgt ggcggagcac agccgacaaa cctatgagcg ataccgggag
     1201 ttgttgtctt cgggggttgc ccgagaactc gccaggatcg tgttgccatt aaacctttat
     1261 acgctgtggt attggacggt caacgcgcgg tcgttgatga actttctctc cctgcgcgcc
     1321 gacgagcacg cgcaatggga gattcaacag tacgcgttgg cggtgggggc gatcttcaag
     1381 gaacggtgtc cttggacatt caccgcatat atgaaatata aatacgcggg gagattgttg
     1441 gagcttctcc agagggagtg tgattgagat gaaactcatc gatatttcga aaatattaga
     1501 ggcaaaggtt tactccggag aagaaaagtt gaatgatctc gagatcgatc gagccggagc
     1561 ggccgatatg atgagcgacg ttttagcctt tgcgaaaccg ggtatgttgc taatcaccgg
     1621 gttgaacacg cctcaagtgg tgcgaacggc cagtatcgtc ggcatagcga cggtcgtgat
     1681 cgtcagaaag aagcagatac cggcggagac gatacaggcg gccaagatgt tcggcattcc
     1741 tcttctacac tctccgttac cgatgtacac cgcctgtggg gtgctgtatg cgcacaacct
     1801 caaggatatt cgcgcgttct agttaaaagg ggggcctatg aaaccgacag tcgataaact
     1861 gatagaccag atgcaaatgc tgtttaagga tttagcggcc aaagacctga tggtcccgaa
     1921 tgtcatttcg atcagccccg accgtacgat ggaacaggcg aaggagttga tgcgattaaa
     1981 aaagatatcc ggtctgccgg tcgtcgaaaa cgacatcctg gtggggttgg tcagtatcga
     2041 agacatcatc aaagcgttgg aacggcatcg gattcatgac ccgatttaca aaatcatgac
     2101 gaaaaacctg gtcgtggtga gtcctgacga gactctgccg cagatattcg acaagtttac
     2161 cagaacgggt tttggtcgat tccccgttgt ggacgaccaa aaacgattgg tgggcattat
     2221 cacgaaagag gacatattgc gcgggatttt ggaagagttt cgcctgctct atgtccggga
     2281 taaaagacgt caggatgtca tcgacggaga ggcgctgaac cgttcgttga tcacgggaga
     2341 ggagatcaac aagttgggcg cggacttcgc cttttcgatc gattacgtcg aagtcagcct
     2401 atccggagtc ggcgccgcca ggttgaagga tttcctgaag ggcaggggca tcgaagaaga
     2461 gatcgcacgc aaagtcgcta tcgcctctta tgaggcggaa accaacgtgg tgatccattc
     2521 ggggagctcc ggaaacatct attgcttcct ctacccggag tacatacgcg ttcgagttga
     2581 ggaccacggg aaaggcatcg aggacttgga caaagcgatg caggaagggt attcgacggc
     2641 gactgatcaa attcgcgaga tgggcttcgg ggctggaatg ggtatatcca acatgcaccg
     2701 ctactccgat aagatggtca tcgtctccga ggttggaaaa ggggtaatcg tagagatgca
     2761 atttttcttg aaagatgccg ccaacagtgc ctgagggggc gttaacgtgc caaaggggtt
     2821 tccaaaagag ttcttttggg gaacagcgac ggcggcctat caggtagagg gcgccgacgc
     2881 tgaggacggc aaaggccctt ccatttggac ctccttttct cgccaacccg gacgtatcgc
     2941 cgacggacac accggagata tcgcctgcga ccattaccat cgcttcgaag aggatttggc
     3001 gttgatgaaa gaactgggag tcaacgccta tcgattctcg atctcttggc ccagggtttt
     3061 acccgaagga aggggacccg tcaatacgcc ggggatggat ttttatcaaa gatgggtcga
     3121 ccgcttgctc gaaaaccaaa tcacaccgtt cgtcaccctc tatcacgggg atctccccct
     3181 cgccttacaa gagaggattg gcggctgggc cggagaggat atcgatcggt acttcggcga
     3241 ttacgcggcg ttgatgtttc gtacgctcgg tgatcgtgtc acatactgga taacccttaa
     3301 cgaaccgatg cgcttctcct tcatcgctta cggaaaggga gagtacaccc ccggtgagca
     3361 aaacctcaaa acggccttcc ttgtcgccca tcacctgctt cgcgcgcacg cccaggcgac
     3421 cgcgcgcctc cgcgagctgc taccgaacgg aaaggtcggg ttgatcaacg cagccagctg
     3481 gatcgaagcc gcgagcgaga gcggagagga catacaaacg gccttcatcg cgaaccagtt
     3541 gctacacgat tggttttttc ttcccgtcct caccggaaag tatccggagg aagcggcgca
     3601 gttcgtacgc gggctgggac tcctgccggt ctttgacccg caagagatga gtcgtatcgc
     3661 gaaagcaccg gacttttggg gtattaacta ctatacccgg cgtcgcgtga aaatggatat
     3721 gagtcggccg atattcttcg cctacgcacc tccagaacgc gaaaccaccg aatccggttt
     3781 ggagatctac ccgcaagggc tcacggcgtt cttaaaaata gcgcaaaaag aatacgggag
     3841 cattccgatc ttcgtcaccg cgaacgggat ggcggataag gacgtcttaa gtaacggcgt
     3901 tgtcgccgac gaaaggcgta tcgcttatct gaaatcccat atcgaggccg ttcagcgagc
     3961 gatagccgag ggagtggacg tgagaggtta cttcgtttgg tcgctgctgg acggctttga
     4021 atggacccag ggctacgaca aacgatacgg cttgatctat gtcgactacg agaagagcct
     4081 gacgcgtatt aaaaaagaca gttattacta ttacaaaaat tatttggccc tctagcgaag
     4141 gagaaagcga ggcggaagat tgaagaaaac tctattggtg ttcatcctta tcggtatgct
     4201 cgtgttcttg accggttgcc agttcgaacc ggaagagacg taccgcgttt cgaacttgat
     4261 tgagatgaga atcctaccct acacgcgaac ggagctcaag ataacgaccc cggctaccgt
     4321 tctacagaag aaagacacga aggacggcgc actggtgttg tatggcacca tcttcgaggc
     4381 ggtttatacg acgcccaacg cgaaagaaat caagctgacg gtcggcagga ccgataccct
     4441 gaggattgcg gacgagctat ggcgtcgata ccggaaagaa gacgcacccc ctttctggac
     4501 ttgggtcaac tacctcccgt tcttttatgg ttcctacgaa acacagagcg ctacgaagtt
     4561 cgcgatggtc tggttctcga aaaagtgggt ctttcggatc gttggacccg acgaacaaac
     4621 cgttcgggac ttctaccgta aactgacctc ttttatcgaa aaaaaagagg tgttcaacgg
     4681 gagcatacgg gagatttgaa cctctaccat aaacagaaca cggccctcga agaaatatgt
     4741 tgtcctttcg agatatcgtc gcgctcgacg ggtccgactc ctcgataggt gtcgcccccc
     4801 cacggacggg ggccatatgg aaggatgaca caccagaaag ctatgaatca aggagataga
     4861 gacgcaaatg acgaaaatgg agacctggca aggcaagagg attcaagccg cctgatgata
     4921 gaccggcgtt ttgtcgagag gttgtaccgt ttggagacca cggttcgcgt caacgaagcg
     4981 ctttcgaagc atacgacctt cgggataggg ggccgggttc ctttgtacgt gcaacccctg
     5041 gaacggcgcg ctttaccgga gatcgttccc ttacttaaag agagcggccg ggagtgcaag
     5101 atcttgggta tcgggtcgaa cgtgctgccg gatgacgggc cgttgtcctt catcgttttg
     5161 gatacgacgg gcttgatcgg ttattctcaa gaatccgaag gctttcgggt agaggcgggt
     5221 tatcccctca ggcagctcag ccgactattc tacgctcgcg ggtggtctgg cttggagttt
     5281 tccgcgggga ttccgggaac ggttggtggc ggcgttctga tgaacgccgg ggcttacggg
     5341 tctcaaatag gcgatctcgt gctcgaagtg gaagcctttg actgggattc cgagtgtctc
     5401 cgcgtctatc ctcaaggaga atgcgccttt tcttaccgga gcagtcgatt ttctcaaagc
     5461 ggcgagctga ttacgcgggt gctgcttaaa acgccgcccg gagagagggc ggagatcggg
     5521 gatcggatga gagaattcga gcggcaacga ataaataagc agcccttgga acaaggaagc
     5581 gctggtagca tattcaagaa accggcagca gacttccacg ttggaaaagt gatcgaggag
     5641 ctggggttga agggacgccg ttacgggggg gcacgggttt cctcgcttca tggcggtttc
     5701 atcgtcaacg aaggttctgc gaccagcgcc gatgtccgcg cgttgatcga tttgatacga
     5761 acaagggtac accaggcgta cggaatcgag ttagaactcg agatacgcct atggagccat
     5821 tgataggcga ttcactctat agggttaaga agagagaata aagggggtaa ggcctacgtc
     5881 ttagtctcac ccctgtaata cccccttgaa aagagcggag aaacgaaaga atctccgctt
     5941 tttctatggg tttttcgttt ttcgtgcgcc ccaatgtgaa taagaaaagc gatacccgat
     6001 aaacatcaag acgagcagcg ccccgacgaa gaccaaaatc ccgttggtgg ttaaggctgg
     6061 cgcttcgttg gcgccggaag gtttcgtaat catcccgaaa aggccggggt tggactgccc
     6121 caataaaaac aagaggtaga aatcgaggat aagggcaccc gacaggatgg acaggaagga
     6181 taccgtcttt tcgtgcgaaa ggatcgcaaa gatcccgacg atgagggcga acacgccggc
     6241 gaagacccaa acggtccact gaggtatctc gaagctcggg gtctgcaggc ggatttggtc
     6301 gataatccaa ggattggcga tgttccaaag ccaaacgccg atgaagaaac cgatcagcac
     6361 cccacctaag aagaaaacga acttaaacaa ccctaggacc acaacggcgc aaatcacgcc
     6421 gacgaaaaga gcgatgggta ggtctaggga cggattactg accaataatt cgttcaccgc
     6481 cgggattgtc ttcagtaagg gcatcaggaa gaacaagccc ccaacgaagc ccaacgcggc
     6541 gatgagcact ttagggaacc atcgactggc aaaaagcaac gcgatagaga acggcagggc
     6601 gatgtaccaa taggtaatca aaagttgaaa aaaaccgtaa atatctgtta cagaaacggt
     6661 ttgggtaacc tcttgcatcg taactctccc ccttaagcgc taacgttttg gtatgttcat
     6721 tcaaagagca tgctcacgta accgaccacg cgctgatagt ctccggtgat ctcgccactt
     6781 gtcgcgtgtt tgagaagttt gggcttccat tcaccagaag acatcaacgt tgcggcgggg
     6841 ccgaagccgc acatcgtaat gctttgttca gatataacgg agtacaggcg ctcgacgttc
     6901 atcgagagaa tcgcgtcgat cacttcctga tccttacttc gtgtgacgct ttcggactca
     6961 taatggttaa gatcggtaga agcgatgatc aatagcggtt tcaaggtttt caggaaagag
     7021 aactgattgc ccaagtccaa tgccgtctgc ttgcgttgat ccatcataga gacgggcagg
     7081 atacgaaaat cctttccata gatatactga agcatcggta ggttcacttc cagcgagtgc
     7141 tcgcccatat ggccctcaaa gtctccggag gcgtaattat accgggacaa cagtaggtcc
     7201 gccaattttt cgtcgacttc gacttggccg aagggagttt cccacgatcc tttgttccag
     7261 atactgatcg gttttcccat tccggtatgg gaggggccta tcaagacaat cgtctccggt
     7321 ttgccccgtt tggctaattc ccagataccc caggcgctga ccgagccgct atagacgtat
     7381 ccggcatgtg gaagaaccgc cccgaggctt ttttgtaggg gtttggtggg gaagtcaggc
     7441 aattggcccg ggccgatcgg gcctagaaag agcttctcaa tctggtttct gagcgctatc
     7501 aaattgtctt ggtagaagac tcccgacact acaggttttc gacgcaacgg attctcacct
     7561 ctcttttgtt ttggaaaatt caactgtacg gaatcagcgc ccccctttat gaacgttttt
     7621 tcgccgttcc attaatcttc cgctatagat tatagcataa ttggatgatt cagatatctg
     7681 cgtaagtgga aaactcatag agccggagga ggaaaaagcg ccttcgcaaa tacaagggag
     7741 ggtctctgtg agcgctggat ttctgggaac gccgaactaa agttcggtgt tcccagcaac
     7801 gagggatgcc gggttcttcg cactgtgtag ggttttatgc tacaatcgga ggtgaagacg
     7861 cgttttcgtt ctgaagaggt gattatgcgc cgggcggttg tctcatgttt cctcttgttt
     7921 tgttacgtca gtaccctatt cggccttgag gcctccctat ctttctatct cttgtccaaa
     7981 cacgacaagg ttgtccggaa tttcgggttg gctggagggt tccacaccga ggtctataga
     8041 gacgacctcc agatcgtgct cgattggcag ctgcgaaact tccaagcggg tgatttcaag
     8101 atcaatttcc cgctcctcgc gggcgcgttc aagtcggata aaccgatcta ctattatcct
     8161 caagagagct ttttgggcta cgataacggg acttcgcagt tcaaaatcgg gtattttccc
     8221 ctgaacgaag gtgcgggaca ccactacccg ctctggattt cggataactc aggcgcttat
     8281 ccagccatac gcttcgaatg gaatccggaa cattggttca ctttcacgaa cgcgattctt
     8341 tttatgaggc agggtgtgca ggattggaag tcagagaaca accttcaaac ggcgaaaacc
     8401 ctctactaca ggaaggcggg tttccggccc ttctactgtt gggagttcgg gttcgaagag
     8461 tctatgctct tcctcggccg cacgctagac cttccctacc tgatttcttt ttttccctac
     8521 cagtctatgc aagaacttcg taacaatttc gacgcccctt ggaaagaaaa attcaacgac
     8581 aacggtatga tcggattcta tacgaagttc tacctggatc cggtagacgt gtacgcctca
     8641 gttttcatag acgatttcaa ccccacaaac ttgaaagcgg cgaacaacaa gcttgcctgg
     8701 aacgttggtc tgaaatggtt aattaccgac cgtatctctt tgtctttgga gagcgcggga
     8761 gcgtcgaagt acacctatca gcggcacagc ctctctgtcc cgccttatat gtacgtacgc
     8821 tatgaaaccg aagcgaccct gccgatcgag tacaatatga tcgggtatcg gtacgggccg
     8881 aacagcggtg tgttcaatgt ggaagcggcc tatcgggccg atagatgggg aggctctgtg
     8941 aactatgaat ttctggtttt cggcaaacgg gcgcccctcg agcagtggga cgagcatggc
     9001 gggactatgc cggttctcac cggtctgcct tggctgaacg atccggtctt gcagtacgag
     9061 aacacgatcg gactgaatct ctacggagat gtcttagaaa acctccggct caatggaagt
     9121 ttggggatca ccttcgtgaa aaacgacggc ctcgagaaag ggaagagcga cactcgcttt
     9181 tctttcgcct tcggcgctca agtgaagata cctctgaccg ccctgattga cagattatgg
     9241 taaaatattg ggtaaacgag actcccatag agattgtatc ccataactga atcgccggat
     9301 ctcacggaga tctttcggtt ttaaaccata aggaggcaaa cgcgctatgg acaaactgga
     9361 ccttctgaat atcgcgctac gtatcgaatc aaccgggtac gagggatatg cccagctcgc
     9421 ggaaaaaacg aagggtaaga tgaaagagtt cttttcacac ctcgcggcgc aggaacgcga
     9481 acatttcgaa acatttaaaa cgatcttcca gaaaatcgaa aagcacgaag aaccccaaag
     9541 ctggggagac ctcgaggcgt tgggctactg gcaagcctac gcccaagtcg cgatctttcc
     9601 gaaaatctcg aaccccaaag tcccggctac aatgcaagaa gcgatccacg ccgcggtcga
     9661 agcggaaaaa gagagtatca ttttttacaa cgggatcaaa gagtacttcg aggataaaac
     9721 cgaaattcaa aagatcattc aagaagagca gaaacacctg ctcgatctcc tcagtttgcc
     9781 ggacgcgagg aaataaaccc ggccggattc gcgcttccct ctggcgaggg acgtatcgat
     9841 agaggaaacg agagtttcct ctatcgtttt tctatggttt catctcatct ccggagttac
     9901 gctttcgtca tcgatcttcc gaagctcgac cggaggttcc ttcacccacg agacgcccac
     9961 gcatccactc gtaggtaggc ccttcccata ggaaaagaga gaggtcgata gcgcccctct
    10021 ggtcgaccgt tacgccttcg agcttcaaca actccagttg gagttgcgcg ccctcttgct
    10081 ccaagctgct ccttccttta ctgttcacca cgcgatgcca agggagagtc ggatctttgg
    10141 agctgtttaa agcatagccg actgcgcgtg gtccgcacac gcccaagata cgggcgattt
    10201 gtccgtacgt cgccacgcgt ccccgcggaa tgtgtcggat gacctcgtat atcctttcgt
    10261 atagcgtcat gtcgcgccct tctttacgtg ctccttcgtt tcggttaacc ggtcaagatg
    10321 atgtcggcga tgtttttgga ggcctctcct gagccgtaag gattcgcagc gcgactcatc
    10381 cggtcgtacc gttccgtgtc tttgaggagc gccatcaccg tgtcatagac ccgtttcggg
    10441 tcggtccccg tcagcagggc agtccccgct tccaccgcct cggggcgttc ggtcgtttct
    10501 ctgagcacca gcacgggttt tcctaaggtc ggagcttctt cctggatacc gccggagtca
    10561 ctgaggatga tcttcgaacc ggccatcaac cccacgaaat cgaggtatcc cacggggtcg
    10621 cagaggatca cccgcgggcg gtcttgtaga agtggaaaca cgacttcgcg gacgcgcggg
    10681 ttcaaatgaa cggggaagat gattttcaat tcggggcgat cgtttagtat acggagcagc
    10741 gcgccacaca ccgcctccat ccgctctccc cagttttcac ggcggtgcat cgtgaccaat
    10801 agataggacc ccttttccag tgcataggtc ctcaatatcc gttcggtcgc ttccctttta
    10861 tgccgtagaa cccataggag cgcgtcaata acggtgtttc cggtgatgaa gacttttgaa
    10921 ggatcgatcc cctccctcaa taaggactct ttggcgtggg cggtcggcgc gaaatgccaa
    10981 ctgctcagct gcgtggtgag ccgtcggttg atctcttcgg ggaagggatc gtaacggttc
    11041 gcggtgcgca acccggcctc cacatgcccg acaggaatct tgtgatagaa cgcggccagc
    11101 gcgccgacga aagtggaggt cgtgtcacct tgaacgagca cgacgtctag gggttgccgc
    11161 tcgaaggcct ctcccaacga ctgacacaaa cgagaggtca agctcgacag gctttgtcgt
    11221 tcggtcatca cgttcaggtc tatgtgcggc tccaaaccga agatttccaa tacctgatcc
    11281 aacatctggc gatgctgcgc agtcgcgata atcgaggcct gatgtcctcg cgagacgagc
    11341 tcgagataga ctggggccat cttgatcgct tcgggtctcg ttccaaaaat caaaccaata
    11401 cgcatcctct gcctcctcca tagtaaccta tttcacatca atataacgat ggctcatcga
    11461 ttccgatcgt gaaggtttta ccttagtgag cgcgtgttcc cgcagagtta ccccacacta
    11521 ccccacgcgc acgcgctttt ttagtgcagc gatctcgtcc gcggaaaccc cgtgcggcca
    11581 ccaatatgga aggatcgtat tgttcagaag caccgggtcg acctctcggg gtagttttcc
    11641 gctttgaacg aggtcctcgt actgtgcggt ccgtggaatt ggcgtgtatt cgttgaggtt
    11701 gaacccgatg tctagcctct cgcacagccg gactgccgcc tcaacatctt cgattgactg
    11761 gctggggagg ttgaccatta ggtaagcgct gacctccgtt ttactaaacc cgacggaacg
    11821 caggatctct atggaacgca cgaattcttc cgtcgtcact tttgatcccg tctgagcttg
    11881 aagtgcgagg tcgagggttt ccaaccccac cttgatcgtt tggacattcg cctgccgcat
    11941 caacctcgca gtttctttgg ttaacaggcg cgcgtgtatg ccgttgggta ggtgaaagct
    12001 gatgcccgaa gtcggaacgc cccgttggat aagcattttc aagagcaggt caaaatgcga
    12061 cgtgtggtcg accagaaggg cgtcgtcgaa aaaggccaca ttttggcatt tgtgagattg
    12121 gcacgctgct tctatagcga cggccaactc ttcgaacggg tgttggaaga aacggtgaat
    12181 caaccccgta gcgcagtagg agcaatgaaa gggacacccg actgacgtta ggaaaaccag
    12241 atggctcatc gcgcctggat acaggtcgta cagcggaatc agcgagtccg gcccttcgat
    12301 ggtcgagaca ccctgcgcct ctagaaagac ccgaacaccc acccaatccg agcgtatgca
    12361 gatcgcgtcg gcgccactca aagcggcgtg gtcgggtagc aggacggggt agtttccgcc
    12421 gagtagaact cgcgctctcg gtagggtttc tttgaccaaa cggatcatct tccacacggc
    12481 cggataccag taggtcatcg cggaggtgac catcacgatc tcggggtccc accgctcttt
    12541 caactccctc aagcggcgcg ccaccaactc ctcgggccaa ccatacagtt tgtaccgtcg
    12601 aggcaaccat ttgatcgctc gcggcttttc tatctctcgg cagaggaatt tgcccgtgcc
    12661 gttggctttt gatcgcggaa gctttcccgt cgcacgcgcc aattgcgggt cgtatcgatc
    12721 caaacaatcg atgaggtgta actcatgacc agatcgggag aggtatcctc ccaagatcag
    12781 cagccccacg ggtttcaacc aaaaatcata agcggcgaca tcggcaatcc acggattgat
    12841 caggagaatc ctcatctcgt tcctttcgaa tactgtattg cgcgagtttt aaggcttggt
    12901 gcccgcttcc gggcagaagc cgagagatta taccacgtca tcgggtgtag tataatacag
    12961 gcgtcaaaga aactggttca caacggactc tctggaggtg tcagatgttt gttttaggta
    13021 attttctttt agcgctggct caaatcatac agctgattct atacgcctac atcttcatcc
    13081 tcatcctcaa ctcgatcctc agctttatgg gcaacagcta tcagttgtac cagattaaaa
    13141 aagtcgtctc aggattggcc gggctgatgc tcaatcccct ccgcaaggtc ttcaaaccca
    13201 tcgggaacgt cgactacacc ccgttcatcg ccattttaat cttggtcttc atccagtctt
    13261 ttgtcgttca aacccttata gatataggct caaggttgag atgagcgtcg cggtcggcat
    13321 ccttttcaac ccacacaaac ccgcaataga gaagtatttg gatatattga tcgagcgatt
    13381 ggcgcgcaaa gggctgaaga cgccctttgc cctttcgacg gaagggccgg agttcgtttc
    13441 tcgttcggaa tttccctcac cgatcgatta cttgctggta ttggggggag acgggagcgt
    13501 gttacgcgcg atgcaagcga cgcttcagaa acagataccg gtgatcggcg tgaacttcgg
    13561 acggtttggg tttctcactc gttttgagat cgaagaggtc atcgatccgg acttcaatct
    13621 gtccgaaatc actgtttctc gccgcttagt cttgggtgtt cgggctctca agggagtgga
    13681 gagcctgacg gggtacgctt tgaacgatgt ggtcgttcat cgccccttag agcgcaacat
    13741 cgagcattat cacatacgga tcaacgggga gtcgctgccc ttcatcgccg gcgatggcgt
    13801 gatcctctcc accccgaccg gatcgacggc ttattccttg tccgccggcg gggcgatcat
    13861 ctatccgcaa gcctacgttt ttcaaatcac attgctggcc ccgcacacca tggcccgatc
    13921 tccgatcatc ttggacgacg gttccgtgat ccagctcgag aaaaacccgc cgagttctct
    13981 ttattactcg ttggacggat tcaccgtcaa gaccggagat agcctggagg tcaggcgcgc
    14041 tccgtatgac tttctattgg cttcaccgaa aggagaatcc ctgtcggcga tcatctcaga
    14101 aaagtttttg tggggaaggc ggggttaacg gtgctgcgcg aaatgcgggt gcagaacgcg
    14161 ttgttgttca aacaagccga gatggccttt gaagacgggt tctgcgcggt aaccggagaa
    14221 acaggggcgg ggaagtcgat gtttgtttct ctcttgcgct ccttgtccgg agagttgaag
    14281 ggaagggacc ttttgggtcc cttcggagac cggttttcaa tcgaaacgtt gtgggatggt
    14341 tccgagcagg tcacgcggct gttgtccgaa aacgacctgc cacaagagga tggcttgctg
    14401 ttgaaagtgg caggaacgga ggagcggttg tcctatcggg tcaacgggtc cttggcttcc
    14461 gccaacgtga tgcgcgaaat cgccaacgcc ttggtctcga tacattccca gaacgccttt
    14521 caaaacctga ggaacccgct ctttcaaacg gcgatcgtgg acgggtaccg cccgcaagcc
    14581 ctggaagaga tcgcgcgccg atatcgcgag gggttccgaa ggtatcagga gttgaaagcg
    14641 ctgtccggcg cgctacccac ggatacgcgg gagatggcgc gcgccctcga ctttttagcc
    14701 tttcagatag aggagatcga aaaggcacgg ttggtcgtcg gcgaggacga gcgggtcttc
    14761 gcggagctga gggtgctgtc caatttcgaa tttcttaaaa acaacctctt aggggcttta
    14821 gcgcaaatcg agggagaagg cgatttcgat tcgatcgcgg accgtttggg cgagatcgcg
    14881 tccaaactga agagggtcaa cggcatcgaa acagcggtcg agggttggga gaagctgacg
    14941 gtagagctct cggataccct acatcaactc cgttcggaga tttcggcgta cctggaacgg
    15001 atggactacg acgaggggcg gctccgagag gtcagcgctc ggtacgatct gatcgaaagc
    15061 ctcaaacgaa agtacgggaa ggatattccg gatgtgttgg cgcgactgga ggcttttcgc
    15121 gaggaaaagg cagcgctgca agagaaaatg agcctggccg agcatttgga cgaaaagata
    15181 gaagcggcga aacacgtact ctcgagtatc gatgcagagt ggcgcgccct tcgagaaaag
    15241 aacgcggccg ctatagaagc gagcgtggtc cgggagctgg cggacctgag gatggaaaag
    15301 acacggctcg cgcacgaaat ctctccggaa gaggatttca cggcacacgg gggccatacc
    15361 ttggagtgga ccgcttcgat caaccccggt atgccttttt tgccgatatc gaagatcgcg
    15421 tcgggcggag agctctcgcg tatctttctt gcattggaac tggcgttgaa ggagtcttta
    15481 tcggtaaaga cgctggtctt cgacgaagtc gactcgggtg tcggtcctcg tatggggcac
    15541 gtcgtgggag acaagatcaa acagcttgcc caaaacggct tccaagtgtt cgttgtgacc
    15601 catcttccgc aagtcgcggc tcgagcggat caacatttcg ttgtccggaa gatgtcagat
    15661 gaggcgcaat ccctctcggt aatcgtcgaa gcgagaggaa ccgaaaggga tcgggagata
    15721 cgggaaatgg ctggagaaca ggaggcgcct tgatgatcgt cgcgttcaag cgcgcgacct
    15781 ctttcctcgg gttccttatc gcgaaagatc gagtatcttt cgatctacgg ggctatcctg
    15841 gggattgctc ggttcttttt tcatcacctc accccaaatg tccttgagct ccttcacgaa
    15901 ctgatagacc tcgtcgagta tttttgtgtc tttcttgctg ttcgcttcta ataagcgttt
    15961 gtacaggtaa ttgtagaggt cgcgcaggtt ggtcgcgatc acttgtccct tctctaggtc
    16021 caacgagatg ttcaattcca cgatgatatc ttccgcgcgc acgattttcg tgttcgcggt
    16081 aatgtagtct ttttcggcta tcgcgttttt ggcctgttcg atgaagctga gggctccggc
    16141 gtacaacatc tcgacgagct tcgcgggaga cgcggtgaag atcgactgct cgaaataatt
    16201 ctggttttga ggatgatgaa ataacgtagg ggccggtttc tgtttttgga tgggcggttc
    16261 accgaccgtc gctaccacat tcggcgaagc ggcgggttcc tggacagcgg ggctccttct
    16321 cggaaatccg ggcctaagat tagtgtataa ctgtttgctt tgcaaaagag agcacctccg
    16381 tagcgcttac atactgttcg tttgaagcaa cacggtcgct tcatcgaaaa acgcggaaaa
    16441 ggttttcagt ttatccggca attcgtgaga gagggcggtc gacaaacgca cccaatcctt
    16501 tctgcccatc gattcgttga tttcgttcag tagctcggaa aaggccggtt ccaagccttt
    16561 caaagaacgg aaatccgttt cctggcagcc ggatatcgcg aagatatttt ctttcatcgt
    16621 gaggaccgaa ttcaacccct cgccaagatc gttaatctcg cgcaacgcct ctatgtcccc
    16681 actcaggatt ctctccgcat agaagggtaa ccgagtggtg atgtggttga ggtagttctt
    16741 gacgttgccg atcagttcaa cggtgaaggg tcgaaactct ttgaagacga gctcgatctt
    16801 ttcgtctcct tcgaaaaccg agagctcaag ctctttcaaa gccgagagcg gtatatcgtg
    16861 cccattgatc ttgacctctg ccagtacctt tcccttcact tgttgcttcg cgataaagtc
    16921 cccgaagttc tggtatatct tttcgtcttc ctctttcacg cgcccgttga cactcacctg
    16981 gatcatcgaa tccacactcc ctcatccgct ctttttatcg gaaaaatagg cctccacttt
    17041 agctcgagct ctcaaagcaa gggctaaggt ctttgcgtcg gttatctgtc cttgggcgca
    17101 acgctcgatg aacgcttccc aaggaagtcg ttccacattc aagaactcgt ccggatccgg
    17161 gtgattctcg cccggtctca gatcgaaagc gacgaagagg tagatctttt catcacaaaa
    17221 accgggagtg gtgtagatat accctatggg gatcacttcg ccgccgtgta atcccgtttc
    17281 ttcccgtagc tctcgtttcg cgcaggcgat cggatcctct cctacttcga gccggccggc
    17341 gggcacctct tggagactcc ggtcgatcgg ctttctgtac tgcgtgacga ggtgtacgta
    17401 tccatcggcg tcgaaaggca acacggcgac ggctccgtcg tgttcgacca cctctctacg
    17461 cgccttgctc ccgttctcga gctccacttg atcgaggcgc agccgcaaga tcttcccctt
    17521 gaacacgtat tcgcgaaaga gttctttttc atacaatcgg ttcatacctc tcctttcggg
    17581 acaacacgtc aacctttcag accggttcta gcgattcctt ggatgaaata tttctggacg
    17641 aataaaaaga gaatgatgac cgggatcatc gaaaaggtgg aagccgccat cagcttattg
    17701 tacaatgttc ccacctcggt gctgaaggta gatagcccaa ccgggagcgt tcggacctgt
    17761 gggctcttcg tgacgatgag tacccagagg aatgagttcc aacttccgac gaacttcaat
    17821 agcgctccgg tgatgatgac cggtttcgag agcggcgcca tcaccttcca tagaaatccc
    17881 catttcgaga taccgtcgat cttgccggca tcgtacagtt cgtccgggat ggtcataaag
    17941 tgctgccgca ttagaaagat cacgaacacg ctgacgaccc aaggcacgat gagcgccagg
    18001 taggtgtcca tccacccgaa cctggccagg gtgatgtagt tcggcaccaa aagcacctcg
    18061 ccgggtacca tcatcgttgc caagaagacc atgaagatga agttcttccc gaagaagttc
    18121 aatttcgaga aggcgaaggc ggccatacag gcgaagagca cgtctagaag ggtcgtgacg
    18181 agggcgacga aaaccgtgtt gaaatagtaa tttccgaagt actttcccat aacccaagcg
    18241 tccacgtagc tttgaaacac attttggaag atttctttaa aggtgaagct cacttggagt
    18301 tgctgagtct catcccagaa ccagtagggt tggattccgt ccagtacgat ctcgatcgat
    18361 ttgtaggagc ggaaacggac atccttcacc tcttttggcg cctccaacac gtccatccgg
    18421 tatccatagc gtgagtacac ctcgctatag tactcgacga cctcgttttc gacgaaacgt
    18481 tcgtgaatga ggacatacaa gagggcgtcc ggttgtcctc ggaaaagcgt cttcaggcgg
    18541 ttcaactgat cgacgagcga ccgacgctga gaggcaggaa cggacgaagc gttaagcacg
    18601 tcaaccgggt ccccgactcc caagaacgct ctcataattg gttctttgag ctccggatgg
    18661 tctaaaaaga cggagttcaa ggtgcccacg aagtccttta aattcagcaa ggtcgattgg
    18721 atcttggacg gatcgatgcc cctctcgctc aagtgtgacg taaaaatccg gtccagttct
    18781 ttcagcagcg cattgaccga ggtgtctacg ggatggccct ccagcacttc ttgcgtcaaa
    18841 cgtttgtgct cctgagtaag ggagctgttt ttgatctgtt cgaggatgtc ctgtttgata
    18901 tccgcttctt tcctgaactg aaaccgaagc gtgccggagc tgagctggtc ggtttgtagg
    18961 cgtgtataga atcggtaggt ggtgattcct aatcgtaaaa ggttttgaaa gctcttcaac
    19021 ggctcgcgaa tgttgcggtc gacaaggcgg atcaccgccc agtcttgctc gctcacaagg
    19081 gcttctgcct cttcggcgct cacattcagc gctgcggcga gccccgtgta ggtcaagccg
    19141 tcggcgaagg tctgggcgta cttggcagag atctcctcca aatccgcttg ggataggagt
    19201 ggctgtccct ttctgaaaaa ctgatttgcg acggtataga tccctttttg ggtttcgaaa
    19261 gttcgaacga gtcgctcgag ccagtctctg taggcggctt ttagtagggc agtttgaggt
    19321 tcgtcctgtt ctgaggggag cagggctcgg tgttctacga gtttgacacc gttagaaacc
    19381 aagtaagcgt tcaccttgtt caagacgccg tcggaggagg tcaccacccc gatgcggttg
    19441 aggaacgcgt gactggaaaa gaacagttcg ttcatcaggg aaggcagata gacgatgggg
    19501 tcatccacca tccctacggt gtctaaaatg ctctgtacct cgtcgggcaa tggtcggagc
    19561 gcaccctgct cggtgaggaa cttatcgaat tcggcgcgcg ggatcgcgcg cgcgtaggtg
    19621 ggattcttcc ccgcgccggc gttcaggttc agctcgaaga taccgcgata aggcgtgtcg
    19681 tcgttgacgg agtaaacgag cgtcttttcc cctttcttct tgctcccgat gatgctggcc
    19741 aagttgaaaa actcttcgat cgagagcgag gagaagtcga gggcgacgtc ggcggagcgg
    19801 accaaattga gcttcacgtt tctctgtttg gcgaaactgt tgctggtcca tttgggaggc
    19861 cagctttcca cctcggtgtc caacttgacg gaagtgagga tcatccacgc gaagggcatc
    19921 aacatcacga acgcgccgat aaccagaagg gtatagaaga tcgctttttt ccaagaggag
    19981 aacatcgcta tcacccgacc cctcaaccga cgtattgtac gcgctgtttc ccgacgcgga
    20041 actggaagaa ggtaagcacc aaaataatca tgaagagcac ataagcggct gcgctggcct
    20101 ctcccatctg ctggtgcgcg tagaatctgt caaaaatgta atacaccatc gtcatcccgc
    20161 tgttgttgta aggcccggga agggcttgat acaacacgta gacctgcgtg aacactttga
    20221 agttgccgat gaccgatacg atgaggatga agaaggtggt gggagagaga agcggccagg
    20281 tgatgtgata aaagttttgt aggttggacg cgccgtccac cttcgccgcc tcatagtagg
    20341 acttgtcgat ggattggagc ccagccaaga agatgatggc attgtacccg acgctcttcc
    20401 aaatcgacac gatcgcaatg gtcgggatcg tccaagtctc ctgggtcaac catttcacct
    20461 tcgagatatc gatgagcgac aaaaagtagt tgaacagacc gtactcgtcg ttgaagatcc
    20521 agcgccacac caaggagatc gccacaacgg aggtgacata ggggacgaaa tacgcggtgc
    20581 ggaaaaaggg tttcaacttc agcggtagat tgagcagcaa cgcgatgaac aaactgatca
    20641 cgatctccgc cggagtcgtc aggatgacgt agtaaatcgt gttgaaaatc gatttcacga
    20701 acaccgagga ctctttggtg agcagcagga agtcgaacag ttcgtatcgg actaaggaaa
    20761 tccgcttgag ggcgaagtag aggaggagga ccgacacggc gaggctccat agacccatgg
    20821 gctttttaaa ggcgtaaggc cttctgtttt cgaaaaaata caggctcccc atcaagacga
    20881 ccgtaccgaa gagcggccag atcaagccat tgtgggcgaa caacaaccct aagaccgtca
    20941 gagccgcgta gattgcggtc attctcagga gtcgttcgcg gcccaaccga tgagactcga
    21001 aagccttgaa ataatgggtc cataggaaga cgagacgtgc gagcaggaag gccgcgacta
    21061 tgtagaaggc ggagtacacg caggcttgcc agaaagaata cggcagcgcc atctggagtt
    21121 tgaacagttg cgtgtagttg gtgaagccgt ggaaaaacgg cgccctctgg ttggtgaaat
    21181 cccatttgaa aaagctgagg atgagcgagt aacctatcgg ccaaaacacg aaagcgccga
    21241 ggatgatgaa agcgggcaac aaaaacaagt atgcgtgaaa ggtttcgcgc caataacgtt
    21301 ttcgggcttt gccgacatag acgatgcgat ttttcacgtg gacctaagac ctcccctgtc
    21361 aacaggtgtg gattcagatg ctctatgagc gtccgaaaga actgacgccg ctcttatctg
    21421 acggctttct gaagcacgcc caggatattt tccagcacca ggcggatgag cttgggcttt
    21481 gcgggcaagt tgaatagaat gctttttttg tataagccgg ccaccgcgcg attgacgacg
    21541 gagtattcgt cgttctcgag caagagatac ataatggtgc gttccaggcc gggaagggtt
    21601 ttatcgatga tagacgcggt ggcttcgggg acgacatcgt ccacctcaac gccgatcgct
    21661 ccgaacacga tgatcaaaga gaacccttgc atacataggt cgaaaagggt ttccttgacg
    21721 gtttcgatct cccgctcgac accgatgatc ctgccaatct cgaaccgatt ggctttcacc
    21781 aaatcgagga gctcctcgat gaagtccaaa gggagtccct ttgcgagcac gaaagctatt
    21841 tttccttttg ggggtgtgtg attcatatgc gctctgcctt cccttcctcg acatatcgtg
    21901 cgatagcccg atccacctgg gtaatgatga ggagcaattg ttcgatcagc ttgttgatgt
    21961 tttggtgaaa caagctctca aacaccgatt gatactcgct ggtttcgtct gttaacgcta
    22021 aaatcgtgat atagataaaa ttcgattcat cgtagaaggt cgaaatttgt tggtactttt
    22081 ttaacgccgc gttcaggatc ttgctgaaat cctcgcatat cggtaacagc tcctgacgga
    22141 aaaagtctac ggccttctgg ttaattgtgc gtatgcagga tagaaaatcg cgtaccgtgt
    22201 tttgatcctt cccttccagg atgaaggccc gttcgatctt agaaatcgta tagtactgga
    22261 tctcatagcg cttgctcaaa tacgagcaaa acctcaggaa cacgatatta aacaagaact
    22321 tcaataggct tttcctttcc tcaacctcag ttggattagc cgagtcacag tttccgtcca
    22381 tttctgtgag gtggacaaaa aacgtcgagt tcgtccccaa ccagacctcg aggccctcga
    22441 cacccacggt cggaacgaga tagtgcgtgg tttggtcttt gtactcactg ctctcccggg
    22501 cgaccatatt catcagtgat atgccgtggg cgattcgtcg agactccctg gcgttaaaaa
    22561 tcatattcag atcgctcact ctctggtcga gctcgttgaa tagcgtctcg tcactggcgg
    22621 caaaaccgag aacgtaggtg ttcaacccgg cgaaaaagtg cagctcatcg ataatgtcgt
    22681 ccagggtgaa gctctcgtat cggtagacgt tcaactctcc ccaaacatag ttagagaaga
    22741 tgcgtttgat gtaggccgca atagggtggt cggtttcgct cgcgttgaag gagaaggttg
    22801 tgcttttaag gtccaagtaa gcgaacgcga cgaactcttc agcggaggag cgaacataaa
    22861 ggtggatcga gggcgtgccg aagatgaagg aggaagtggg atcgaaatag gagtcgatga
    22921 gggacaacag aacacccgag ttttcgtcac gaaacagttc cacgaacccg ggttcagcga
    22981 taagggtctc cagcgcgact ccctttacaa acatacgatc cactcacttc tccaatagta
    23041 cgacggcgac cgcgtaacta ccgtcgtgtg aaatcgatac ggaacaactc gcgtattcgc
    23101 caatataggc gcttcttaca gcctcggaga ccctgaggta gggccttccg gtttcatccg
    23161 ccaacacctc tacttgatcg aggacggtat tgaagatgcc gatgcccagc gctttgaaaa
    23221 aggcttcttt ggcagcgaaa cgtccggcga agtactcgat ccggcgccgc gggttcttta
    23281 gcgcctccat ctccgcgatt tcgctcttcg aaaagacgcg catccgtatc cgatcgatca
    23341 tctgaggagt taggcgttcc atatccacga tgtcaatccc tatctttgac atcggcgagt
    23401 cccttcaggc gtcatcggtg tttttccgac cattcgcaat cgtcaaagac cgggcaagcc
    23461 gaacaaactg gtttcaaagg tttgcacacg tttcgcccca tctctaccat cgacccgttt
    23521 aagtcacccc agagggatgc gggcaagagg ctcgtgagcg cgacttccgt ttgttcgggg
    23581 gttttcgtat ggacgaaccc gatacggttg gatatccgat ggacgtgggt gtcgaccgct
    23641 agcgccggta ttccgaaaga gaccgaaagc acgatattcg ccgtcttcct acccactccg
    23701 ggatatcgac acagcgcttc caggtcgttc ggtaccaccc ctcccgcttc gtcgacgcgc
    23761 ttggcgatct cgacgatcca tttcgatttc tgtcgataca accccgattc ctgcaaggcc
    23821 gggtacagcg cctccggctc ttgggccgcc aggtgtttcg ccgttggata gagcccaaat
    23881 agcttagccg aaacccgttc ggtgacctca tcgcgattgc gttggctgat gatggtcgag
    23941 atcaacaccc gaaagggatc ggtctcgtga tgtatcctgg gaaaaaacga gatgatccgc
    24001 tcgcacacgt gtgcgacttg cttggcgtcc atcgtcgtcg ttattctttc agggcttccg
    24061 cgccgttgac gatctcgatg atctcttgcg tgatgaatcc ctgacgcgct ttattaaagg
    24121 ctagcgtaaa ccgctctatt aagtgctccg cattctcggt ggcgtttcgc atcgcatttt
    24181 gacgggagta gagctcgctc acctgggcct ccagcaatag gtgaagcaat acggacgaaa
    24241 ggtacaacgg aataagcgag ttgaggagaa cgttgcgctc cggttcgtat tcgaactcgc
    24301 gctgccattg cgccttctgc gggggtttgg gtttcagggg cagcagatcg gtgatggccg
    24361 gtttctgtat caaagagctt ttgaaatggc tcgagatgac cttgaccgta ttgacctcgc
    24421 cggactcgat gagcgagagt atccgccccg ataacaggtc ggccaccgaa aaatccgggg
    24481 taccgaagaa tctgtccagt gagccgtaga tcttcttgtt gcgatatcgc agatagccga
    24541 tgcctttggt cccgcagatg aaataacctt tgaaggcgtc tccgcgcctt tcttcctctt
    24601 cctcggccat tctgagtata tccaggttgt acgacccaca caagcccata tcggaggtga
    24661 taatcaagaa caggattttc tcttcctcgg gtttcttctt ttccaccaaa gggtgttcca
    24721 aatccggatc cgcgataacg cactcgtcga tcatcgcgct aagggaagcc agataagggg
    24781 tgaacccttt ccacagccgg aagactttgt tgactttcgc tgccgctacc atctccatcg
    24841 cgtgggtgat gtgttgcgta gattccgtgg agtctattct tctacggata gtgtggagcg
    24901 cgcctctgcc caaatcctta tcccccttat cctacgaata ccttcttgaa tgctttgatg
    24961 gcctcttcga tcgaacgagt gagctctgtg gacaaatctt tttcctcttt cagtttcgat
    25021 agtatttccg gatgctggtg tttgacataa cggatgagct gttcctcgaa ggccctgatc
    25081 cggtgggtcg gtatcgtgtc cagaaacccg cgcacacccg cgaagatgct gatgacctgt
    25141 tcttcgaccg ggatcggaga gtattggttt tgcttgagca gctccatcag cttctctcct
    25201 ctggtcaatt gggaccgcgt ggcctcgtcc agctcgctcg cgaactgcga aaaggattcc
    25261 aggtctcgat attgcgccag gtccagacga agggttccgg ctatcttctt catcgctttg
    25321 atttgggcgg tgccgccgac gcgcgaaacc gacagtccga tgttgatcgc cggcctgaac
    25381 cccgcgtaga agaggccggc ttcgaggtag atctggccgt cggtgataga aatgacgttc
    25441 gtgggtatat aagcggagag gtcgttcgcc tgggtttcta tgatcggtaa agctgtcaat
    25501 gacccgcctc ccagcgaagg gttcaaacgc gcggcccgct cgagcaatcg ggagtgtagg
    25561 tagaagacgt ccccaggata ggcttcgcgg ccaggcgggc gacgcaagag cagcgagagt
    25621 tcccgataag aggcggcgtg tttggtgaga tcgtcataaa cgaccaaagc gtcctgccct
    25681 tggtacataa agtgctcgcc tatcgcacag cccgtgtagg gcgcgatgta cagcatagat
    25741 gccgggtcgc tcgcagacgc gaatacgacg gtggtgtaat ccattgcgcc gaactgttcc
    25801 agctgatcga cgattcgggc gacggaggcg gccttttggc cgatggcgac gtagatgcac
    25861 ttggcgtttt tacctttttg attgataatc gtatcgaggg cgatggcggt ttttcccgtt
    25921 tgcctgtcgc cgatgatcag ttcacgttgg ccccttccga taggaatcat cgaatcgatg
    25981 gacttgaggc ccgtttggag aggagtgttg accggttgtc gatagatgac acccggcgct
    26041 ttgaattcta tttcgcggaa atcttgtgtc acgatagggc cttttccgtc caagggctcg
    26101 cctaaggggt tcaccacgcg gcccagcatc gcctctccaa ccggtacgga aacgatttta
    26161 ccggtccgtt tcaccagctg cccctctttg aggttgtggt attcccccaa gacgacgacc
    26221 ccgacggagt cttcgtcaag gttgagggcg aggcctttcg aaccgttttc gaacaggacg
    26281 agctcgctgg ccttcacctt tttcaaaccg tagaccgtcg cgataccgtc gccgacctga
    26341 acgatctgcc ccgtttcttc caattccgcc gtcttctcga acccggttat cttgtctctt
    26401 aatatttgag tgatctcctc gggttttatc ttcactcaag tcacctctct tcggcattta
    26461 ggtgccgctt tgctttcaac aactgggctt gtaacgaaag atccaatatg tggtcgtcca
    26521 cgcatgcctt aatcccgccc aacagatcgg gttgaagcgc gaaagagaaa tggataggcg
    26581 tctcgtatcc tttcgcttcg aaataggcga tgagctcctt ggaaacgaac cccttttgct
    26641 cttcggacaa ggggaaggga gtgaaaatcg tgccctcggt ccctttttgg ctttccgcca
    26701 acaaccggtc aaagaccgga gacaaatcaa ggaggatctt ctctcggcgt ttctccaaca
    26761 cctcggtgag aaaacgctcc atcacctcgc tgtaacagcc cttcgcccgc aacagggcgg
    26821 cgagcgcagc gattttttct cttttgggaa tcgaaggatg aaagacgatg gaacgataca
    26881 ggtccaaggt caaggtttcc tgcaagacgc gcaacgcttg agaagccaac gcggtatccc
    26941 ccgtcagtag ggctttcgcg taaaccagga tgaccttttg ggaggggttt ttcaccggcc
    27001 acctccctgt ttgatcttcg ccaaaaagct cttgaggtac tcgctctgcg ctttctcgtc
    27061 cacctgacgc tccagcagtt tagaagcgag atacacggct agactgaccg acttttcaga
    27121 gatctcctga atcgcctctt tcttttgggc ttctatctct tcttgggcct ttcgtaacag
    27181 gctttcggcc ttctgtttcg catctatccg ctcgcgaatc gcgtagtctt cggcggtttt
    27241 ttttgcgttg gcgattatcc cctccgcctg agcgaccgcg ttgtccaaga gctgtttcgc
    27301 ttccctctgg gaggcttcag cctccaactt cgctttctcc gccaagctga agtcttcctg
    27361 tatccgctta cgcctttcgt cggacatctt gaagaacggc ttgtacagaa acttgtatag
    27421 aaagaacgcc aataatatga attggagcaa catcaggata gaagtcagat taaactcgag
    27481 atgaatcatc ttaaccccct ttctctccgg cgggttttcc tgcgctatat gacaatagcc
    27541 tttgcctaca agacctcaga acgtaggttg ggcgagagag acttttaggc aaaaagaatc
    27601 atgaacgaga tcaataggcc gtacaacccg gtcgtttccg ctattgcgtc ggcgatgatc
    27661 atactgatgc gtaaattgcc cgccatttcc ggctgcctgg ccatcgcgtc catcgcgtgc
    27721 gccccgatgt ttccttcccc tattgcgggt ccgatcgcgg ctaagcccat acagattcca
    27781 gcgcccaaat actttgctgc ttgaacgatc gctgcactca tttccataac tcgcactctc
    27841 ccttctctat tttaataaac cccgtgatac actggggcga cgcttcgtac cactgacgat
    27901 ctcgaactct atttcatacc tgtgaaggtt tgctttctcc gttgttttga tgcggagagg
    27961 atccggtcaa ttgacctggg atccgatgta tacgatcgtg agaaccgaaa agatcaacgc
    28021 ctgtatgagc ccgaagaaca tcccgaaaaa cccccagaag aacataggca ggaaaaggaa
    28081 cttcaggata tagctgagga tgaacacaag gatgccgccg ccaaagatgt tcccaaaaag
    28141 acggcaagag tgggagaccg gtttggcgag ctctccgatg acgttcatag ggaacataat
    28201 ggcgatcggt tcaaaaaagg atttcgccca ggctttgaac cccttcgcct taatgccgaa
    28261 gatatgagaa atgaccagga cgatcagcgc gtaggtgcca ttcgtattga ggtctgaggt
    28321 tgggacatac caggtgtccg tgaagaaggt caaataggtt tcttggttgc gtatcgaaac
    28381 gttgatgccg ggaatcccgc tcagtatgtt ggaagccgcg ataaacagga ataacgtcat
    28441 cgcgatcacg aagaccggtt tgacgtatcg tttgtcgttg atggcctctc ctgtcaagtc
    28501 gtagaagaac tggagcaagt actccaccag ggattgtttt cggttcggca cgagtgaaaa
    28561 ggtcttgcgg atacctctag ccacgccgat caagaatagg gtgatgagca ggccgacacc
    28621 gacggtaagg aaattgatac gggtgaaaaa ggaaccttcg ctaccgggga aggtatacac
    28681 ccatctggtt ccgatgcccg cgtcactcag gtcagtcata ccgcccattt ccggcaaggc
    28741 aagaatccaa accacgatga ttgcaatcac catcgcgatg acaaaggttt tctctgtcct
    28801 actgagggtc atctttctta cctccatttc tcagagcgaa gctgagccaa accataaccc
    28861 atttcaacga aatcgtcccg aaaaaacaac ccaacaaggt ttgaatggag aaaaacgccg
    28921 atatgcccat tgccagcgca tctatcgaat accgtaaaaa aaaggccgaa gcgctcacgt
    28981 actgtgtttt tttctccata cggcgggtct gctcggcgta gacccacacc ttcatcaacg
    29041 ccgcggcggt gcctgccaaa accccataac cgaagggatg ggagaaaaag aaaaaaccga
    29101 ttaaaaagag tccggaattc agatatatga taccatagac agaggttttt agcaaagcct
    29161 ccgtcgtcat cgccttgagg tcgaatcggg ggaaaaaagc gcggaagatc cgccgtctca
    29221 aggcgccccc ttatttttgg ggcgatcgtt ttcggccttc ggctcggctt tggccgactc
    29281 catctctctc gcctctttca acagtcccag gatcccttgg tatagcccgg aggcgatgcc
    29341 gaggacgaga aagatgacga gtagcacgcg gtttttaaag gtgtatttat ccaggaaata
    29401 accgatcgcg ccacccacga aggtgttggc gataatcgta atcccgaaga tgtacaagat
    29461 gttgaggtgc cgaagctctt tgaaggggtt cggctttttt tccgactctt cactcattct
    29521 ctgagaacca tatcgacctc aaaggtctta tcctcatatt tcgtgatgat cttcaagatg
    29581 accccgtcca cgttcacgcg tatctcttta cctaggatga ccgaaggcgg agtgatgtcg
    29641 atgggttttc cgatctgttc catattcgtc gcgatatttc ccgagatcat attggcgagt
    29701 tcaccgatcg cgctcatcgc cagctcgtcg agttggttat acggcattcc catcatcttc
    29761 tgaacgacgc tcagcgccac ttgcgtatcg aaggcgtaga cgaggtttcc ggacaggctg
    29821 ccgttgaagc cgacgagggt gacgaggtcg taccctttgg ccaactcttt cgcgatttgg
    29881 ggcttttcaa attcgatctt catattgacg gcttccgtga agatcttgac ggtgctcttg
    29941 aggatactgt tgatgatgcg gacatccatt ttcatccctc cgttattttc tctcgatatt
    30001 gtatcgcttc agcgagatgg acggcttgga tggtttcgct tccttccaaa tcggatatcg
    30061 ttctcgccac tttcaacact ttgttgatcc ctcttccggt taaatggtat cggttgaccg
    30121 cctgctccaa tagggtttcg gcttctccat ccaaacggca gtattcttgg atcattttag
    30181 aattcaggga agcgttggtg aatcctctgt gatgagtttt tcgaaagcgt cggttctggg
    30241 tctcccgggc cttcaccacc cgttcgcgga tggaggcgga cccttcgccc ccttcttttg
    30301 aagtgtactc cttgtaagtc aatcgcggga cttcgatgac gatctcgatc cggtcgagca
    30361 gcgggcctga aatcttacgg ttgtaattga cgatatcgcg cgcactgcag gtgcaaacca
    30421 ggtccgggtc cccgtagtta ccgcacgggc aggggttttg cgcggcgacc aacgccacac
    30481 tggcgggata ggtcagagtc cctttcgcgc gcgcgacgct cacgatacga tcctccaggg
    30541 gttgtctgag cgcctccaaa acgtcccggc tgaattcggg gatctcgtcc aaaaacaaaa
    30601 cgccattatg ggccaaactc acctctccgg gtctggaatc gaccccgccc cccacgatcg
    30661 ccgcccgaga ggccgagtgg tgaggggagc ggaagggcct tttgcgacgt aggatctccg
    30721 aattcagcaa tccgactatg ctgtagatgg aagcggtctc caagaactcc tcttcgcgta
    30781 gcggaggaag caagccgggc agacgtttgg ctagcatcgt ctttccggaa ccgggcggcc
    30841 ctttcattaa cacattgtgc ccgccggcag cggcgatttc taaggcccgc ttggcctgat
    30901 actgcccgtg tatctccgaa aagtcgtctt ctataggcag gtccggttcc agggccctat
    30961 cggaagggac tctcggaagc tcgcccccgt taaggaagga taagacctcc cacagatcgg
    31021 ccgctaaccg aacgtcgatt tcggggacga gcttggcctc ttctacgttt tcttgtggga
    31081 tgacgatggt tttaggttcc ttcgatttcg ataaggcgat caacaacggc aacatccctt
    31141 ggactctacg caggacgccg ttaagtccta gctccccgaa gaaaaacaat ccggggggaa
    31201 tctccctctc cacgtcattt ccgcaggcga ggatggccac cgcgatcggc aggtccagca
    31261 aggtgccttc tttttttaaa tttccggggg ccaaattgat gacaatccgc ccctgcggga
    31321 tattcagctt attactggaa atagcgcttt taacacgctt tttgctctct ttgatggcag
    31381 tgtcgcctaa cccgacgatg tcgatctcgt ggatcaccgc ttgggcgtcg acgaaagact
    31441 cgaccagcac gtgccgcgcg tgtaaaccga ccaacaccgc gctattcaac tttgagaacg
    31501 acgccattcg gtcaccttcc gatgatcacc ttaccaacct ctttcttgta aatgaacctc
    31561 cagttcggaa atgtctatac ctttcattgc tgggggcagg tcttcgctga tcttggccag
    31621 catattgaaa tcgttccaat gccgtaccgc ttgcacgatc gccgaggcca tcttcgtcgg
    31681 gtcttcggat ttgaagatac cggatccgac gaaaacgccg tccattccga ggatcatcat
    31741 caacgcggcg tcggccgggg tggcgactcc gcctgcggcg aaattgacta cgggcaactt
    31801 tccgagtttt tttaccgttc gaatcaaatc gatcggcgca ccggtctctt tggagacggt
    31861 gactaactcc tcatctggcg tattctgtag ctttcggacc tcttcccaca cctggcgcgc
    31921 gtgcttgacc gcttcaacga tgttaccggt gccagcctcg cctttggtac gcatcatcgc
    31981 ggcgccttcg gcgatcctgc gaagcgcttc cccgaggttc cggcatccgc agacgaaggg
    32041 tactttgaac tcgtgcttgt tcacgtgaaa gcgatcgtcg gctggcgtta ggacttctga
    32101 ctcatcgatg aagtccacgc cgatcgcctc taaaacccgc gcctcggcga tgtgtccgat
    32161 acggcatttc gccatcaccg gtatgctgac cgtgttcatg atttccttga tcaccttgat
    32221 gctggccata cgggctactc cgccggtctt gcggatatcg gccggcacca tatcgagcgc
    32281 cataaccgcc acggcgcccg cgtcctgggc gatcttcgct tggcgcgcat ccgtgacgtc
    32341 cataatcacg ccgttcttaa acatgtccgc aaatccagtt ttgacggtcc atgtagactt
    32401 gctttccatt cttctattcc tccttgtaat gcacttcttt caatcgagtg tagtcaccct
    32461 gccctctccc gtacttcgcg cagggaattc cgtccattct gacgatatcg gccgtgaaat
    32521 ccaccttttc tcgcaaaagg gaagacccga ctccgtacac gtcaacgggc acttccagcc
    32581 gctcgtacag attgatgcgg tcggcgttga agccgccgct caccataatc ttaagctttt
    32641 cggccccgat ccggtcgaat tccattctcg ctttccaaac gagctcgggg gagactccca
    32701 aagaacgttc atcgtgcggg gtaaccgatc ggtcccgtaa agagcccgaa gtgtcgaatc
    32761 gcacacccca gatcttatgt cgcccaggcc cgatgatagt cgacaggtct ttcgtcgcgg
    32821 gagaaagggt tttcccggtc gtgttttcgt aaaaccgctt caacacttct ttcgttgtac
    32881 caatacagtc gttgtcccaa tcgaccaaaa cgatccggtt cacttccggt tctatccatc
    32941 ggtcgaaggc ttccgcggcc cgatatgtat ctcccttgta gcaggcgatc agggcgtgcg
    33001 ggatcgttcc gaggcctttg cttccccagt agtccgcgtt tgcgtcggtc gataccccga
    33061 acgcgccggc tttgaacgcg gcgtagccgt ccgtcgcctg aacccaataa tggtcgaacc
    33121 tcgcgctgaa aaggacgacc ggtttacctc tagaggcgtt gaccactttc ttcacggcgg
    33181 tcgccgtgct cgaagccctg gagatcacgc caagaaagac ggtttccaga gagccaaaat
    33241 agacgggatc gccctcgatc gtcataatga tctccgcgtc tttcgccata tctccgtcgg
    33301 ttaaggccaa aacgcgtatg ctgtcccatt tgtctaccca cagggcattc agcctgtccc
    33361 tcagcgacca tttctctttt gtggcgccgt cggccgcgtg gtaatctctt atatatagcg
    33421 ctttctgcag cttccgttcg gcgtccagga tatcggagaa gatatcggcc gccttatccg
    33481 gttcgttata gtatccggta caggactgta ggatcgccaa ggcttcttct atccccacga
    33541 tacacgcttc cgatcgtggg aagacctggt atagagcgtg atgggacacc ccttctcttc
    33601 gtagcacatc cacgtagcgg gtgaagtact tgtcgctgta gtaacccgcg cggatacgat
    33661 ctatcgggat cttaaagagg ctcgggtcaa accgaggcat ccgccgtacc gtctttcgca
    33721 gcttcttcta gagttacgct cgttggagcc gtatatattc ttccaaaccg acccggaaga
    33781 cttcgcacgc ttttttcatt ttttcggcgt tgatcacata ggcgatccgg atctcttgct
    33841 ctccggcgcc cggcgtggcg taaaacccat cgagcgggga gaccattgtg gtatggccat
    33901 tgtgagagaa atcggagagc atccatttca cgaacttctc gctgttgtcg atcggcagtt
    33961 tcacagaaag gtaaaacgcc ccctgcggct ttttaaaggc caagcctggg attcgagaca
    34021 gctcctcgta caccttatcc cttctcaatc ggtattcgtc tcgaacggag tagatataat
    34081 cctctcctaa cgtcagcaac cccagcgtac cgacctgggc catcgtcggg ggagagaggc
    34141 gagcctgggc cattttcata acctgcgcgt aaacctcttt gttcttcgtc gcgaagacac
    34201 ccacccgggc gccacacgag ctgtaccgct ttgatacgct atcgatgagg atgatccgat
    34261 ttcgatattc gggaaactct agggcgctga agggttgtaa cccgtcgaag acgaactcgc
    34321 gatagacctc gtcgatgatg accatcaaat cgtacttttc cgcgatcttc aacaccctga
    34381 gtacctcgtc tttcgtatac acagcgccgg tggggttgca ggggtttgag aagaggattg
    34441 cgcgggtttt gggattgatc actctctcga aggctttgtc ttccggtacc cggtaacctc
    34501 cttcgggatt ggccgcgaca ggggtcaaac ggacgttcac cataccggcg aaccccttat
    34561 aattggcata aaacggttcg atcacgatga tctcttcatc ggggtcggcg acggcggcga
    34621 cggcgaagac cacggcttca cttccgccgc tggtaatgat gatctcctcg ggtttgaggt
    34681 ccgtctgcca gcgctcgaaa taccgcgcga aagctttgcg tagctccaat aggccggccg
    34741 agtgggaata ggcaacgacc tcttctttgt agttgcgtat gctttccata aagaccggtg
    34801 gcgtcggaat atcgggttga ccgatgttga gaaagaatac gctcgttcct ctagctttgg
    34861 cttcttcggc gaaagggatc aagcgcctga tcggagaagc ttgcatcgca aaaactcttt
    34921 tggataaatt catctttcta acctcccgga tggttcagca ttctaaccgt agtatacctc
    34981 aaacccttcc gatattcaat ctatagacga acgcattcaa acctcgaaaa aaaaaggcgg
    35041 gtttccccac ctttttctcc acagcgcgtc ttaaaccctt cgatcgagga tgttgcccaa
    35101 ccctggttcg gtgggcatct tcggtaaagt ggcgagcaat tgttcgcttt gctcgagggc
    35161 tgcgtccatg gttttcttca ttaccgcagt ctgcacttga ttggaaagct ctgcatttct
    35221 caaagccatg taatccgccg aaatgggctc catattccca cctcctgaca agtgctgttc
    35281 catttatatc ggcagcaaac cgattatact ttattggcta caaggtgcct gtcacgtgtg
    35341 tgggtcaagc tatggggtgt aatcaagaaa caaagccaaa atcaaccgca cgatttttag
    35401 ttcacgtagt catcgtggcg taagcgccct ttcttcatac gacggtacga tacgcatttg
    35461 tacgggctca tcgtggcgta gaaggggcaa agggcagatc gcggggtcgt aaaaatcact
    35521 taccccgcga taacctcaca cgggcatctc ggcgtaaaaa ggataaaagg cggatctcct
    35581 cctcctatct ttttttgttg cgcctcgata ttatacccac gcctgtcacg tcttttccat
    35641 tcagttcgca gaatcaagcg tagtccaaga tataggtttt cggaggtctg atcccgttga
    35701 aataggttcc atactcgacc gtgtacgcgc cggtgttctt gaaatagacg atatctccgt
    35761 aggtgatgtt ggcgggtagg tcgatctgct gatagatcat atcgacgctg tcacaagtcg
    35821 ggccggccag agtgaaagtt ttcttctctt cgtcttcctt gccgtcaacg atcacttcgt
    35881 atcggaagtt ttcgatcgtt tccatcaaac cgtggaagac gccgg
//
LOCUS       DAOPXN010000003        28180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_1646_length_28180_cov_5.259947, whole genome shotgun sequence.
ACCESSION   DAOPXN010000003 DAOPXN010000000
VERSION     DAOPXN010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 28180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28180
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1646_length_28180_cov_5.259947"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            257..1204
                     /locus_tag="PKM49_00460"
     CDS             257..1204
                     /locus_tag="PKM49_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81316.1"
                     /translation="MKNQPIVFRFLWVGAIALAAICIPIVSIRLFLALAAGVLFALQF
                     RKLSPLIIVFLVIAALIIGIGNVIPRFLPLPVWGFGSDLFRNFAYENSYRYNPEVKSS
                     DYSKLIKADTTVNGAPVVIVRVPGVKISFDKDSDKISFPSLLKARTSGSQLTFEAGNF
                     PQDSAAEIVIGSKTPLDRLSLDVSFSEISADEGVFTVKRLEIRTNYLSFDGALVCDDI
                     NVTTNALDWRGSFEAKEARMQSNAASIFVTVRGMENLVLTAQVVYGEIKYLDRWEGSR
                     KARITGTFGSVTFMKPKDSTGKLELQNSSQMVSLQIQEY"
     gene            1220..1798
                     /locus_tag="PKM49_00465"
     CDS             1220..1798
                     /locus_tag="PKM49_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81317.1"
                     /translation="MLPKIEAFFMKEMKEKTGRLAILSLFLLVPFYPFRIFFLFLLCT
                     HMLGWDVQHKRYSSLMALPYMPKQLFWLSYLFLVVISVETQLIGAALGAGVFNMGWAP
                     LGIHLLGSLLFVTSYYGISMLAVTFGLDHFAIPLLVFIVDLTLGGIGFTTNNPYSLIS
                     PVHQKSLPLAIIPAVALLLGSAYLFEKKGAYK"
     gene            1795..2613
                     /locus_tag="PKM49_00470"
     CDS             1795..2613
                     /locus_tag="PKM49_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81318.1"
                     /translation="MNVQIKGLRHAFGAKTVLNGISFEVKEGEIFSLIGPNGAGKTTT
                     LRCLYGDIFPDGGSILLNGQRVSSKLKEKIAVVTESRFSFSRFTGEDYIKLWKALYPS
                     WDEKVFGSFINHYHFELQARADTYSMGWKTLLNLALAFSSGADLLILDEPTQNLDPLI
                     RQEVLNILHTFVAREGKTIIISSHEIFELEEITSSFAVLREGDILYTDHLDDAKENHR
                     IVSQSEKVSYGEVIGLVKNETLIRTTEEVGRYPTFQEIILGYLQGKTRFLPFSR"
     gene            2630..2989
                     /locus_tag="PKM49_00475"
     CDS             2630..2989
                     /locus_tag="PKM49_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNY81319.1"
                     /translation="MLQPVEKHSGVPAYVQIIHMIKKEILMGNMTESSRLPPVRELAA
                     IFEVNINTVLKALDKMSQEEIVQSEQGVGYFIRKTQDVNREAMPVLGEAIRKLKQYGI
                     GLEMTKLLLDELWRTDP"
     gene            3800..4087
                     /locus_tag="PKM49_00480"
     CDS             3800..4087
                     /locus_tag="PKM49_00480"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNY81320.1"
                     /translation="MKTIGEVIDGLSRKKGVFSEIKVRTIFTRWESVVGPLLAEKTKP
                     VSLSRGVLTVWCAESVWASELKFYTKDILAKMKEQLRDVRDIRSIKIVYKE"
     gene            4093..6042
                     /locus_tag="PKM49_00485"
     CDS             4093..6042
                     /locus_tag="PKM49_00485"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase subunit B"
                     /protein_id="HNY81321.1"
                     /translation="MSKDYGAKNIQVLKGLEPVRKRPGMYIGSTGKAGLHHLVTEVVD
                     NSIDEAIAGVCKNIEITLFEDGSVEVKDDGRGIPVGIHEEEKKSTLELVMTVLHAGGK
                     FNKESYKFSSGLHGVGVSVVNALSEWLQVWVMQGGKLYTQRYARGVPLDKVQEVGEAE
                     GHGTIVRFKPDPTVFTTTQFEFEVLNNRFMELAFLNPGLTIKLKDLRENEEIKYCFEG
                     GLTEFCAYLSKGYKNLHPKPIYLSGRYNEIEVEIALQYILSDEEIMRTYVNDVNTIEG
                     GTHLTGFRTALTRLVNDYIKKFSLLSNGRQQKTKKVEKKTEEEELVIQGGDIREGLVS
                     ILSLSVPEPQFEGQTKSKLGNEEAQEAVAFVVRERLSEYFDMYSDVARLILDRIIQAA
                     KARLAAKKAREMVKRKNALEYSTLPGKLADCSTKERAKSEIFIVEGPSAGGSAKQARD
                     REFQAILPLRGKILNVEKASLEKLLKNETISDIITAIGTGIDDTFDIERSRYGKVFIM
                     TDADVDGAHIRTLLLTLFFRHFRPMIKAGMIYIALSPLFRVAHGKDEYFVYTEEEMRE
                     VTNQLREAGKKNISVQRYKGLGEMNPEQLWKTTMDPQKRRMARVSLEDPELANSILMT
                     LMGSDVEDRRRYIQHHALQVTNLDI"
     gene            6058..6774
                     /locus_tag="PKM49_00490"
     CDS             6058..6774
                     /locus_tag="PKM49_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81322.1"
                     /translation="MRLRALSGLTILLALGVCVILCSWFYRKTFEKRNVPIERLLILN
                     AVGEPHGSSGINNPARTSSSSLENQLSRYAGFGYLFYMDGLNRVLRPLVGYEWAGQEI
                     YNGKITLLLREQQAVFLSETEDGERWLVTDQGRRFWRVHPEDERIYADLPVFFGGTWK
                     EQEPVIARFLTYSMTFRNYVSSVSVTEKVLYIRNGVLLIVNQWENLDRLNHNEFIRTM
                     RQKTAYDLYSNGLFFPINRK"
     gene            6788..8170
                     /locus_tag="PKM49_00495"
     CDS             6788..8170
                     /locus_tag="PKM49_00495"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNY81323.1"
                     /translation="MAESTEVLTVVDIGSHTLKGAVAGFEGGRTTILAYSRTKARGFE
                     QGELKDIVALRESLETLLKDLGSQLSKRVDSYFIISFVEKTTFLSEETRNLEISPREP
                     VLINEDHVYELFTRIASQTARKDEPEENYRLLDPRRAVQHVIPQRYLLDETKGVLNPI
                     DMEASVLGMKASILSIDATVKDSLTNIFSGIAGEKPTVFISPFVASEVILNGKEKERG
                     VVCLDIGHSFLTVSGYLNGSVFFLKPLDQGLRHVIKDIAQVFKTSFDEAERMLIQYGK
                     IAIKDTSKDTITYTLLDGKSSKQITRAQLAIVIYAKTREIMNAVKREMRAITLRMAEE
                     GEKGIPGGLVITGAGSKIDGMVEFLRDAFKLPVRIGHAGTGGTDFQLPDELLDPTFSA
                     CAGNIYWFHTIGGLEALEGHALSSSLSTSESEGKPSRKKKSRAVRRPAREKEGPGTWN
                     KIAEFLKKLV"
     gene            8221..9276
                     /gene="ftsZ"
                     /locus_tag="PKM49_00500"
     CDS             8221..9276
                     /gene="ftsZ"
                     /locus_tag="PKM49_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNY81324.1"
                     /translation="MAFEIDDGKDIRRPPKRNPTIKVFGVGGAGNNAVNRMIESGIHG
                     VIFVVANTDLQVLEDAKADIKIQLGKELTRGLGAGGNPDVGEKAAYESKDDILAVLED
                     TDMLFITAGLGGGTGTGAAPVIAKIAKEMGILTIGTVTTPFYFEGVNRWNNAKKGLGK
                     LKENVDTLIKISNNKLLEEFPQDITVVDGFLKADEALHQGIKGISEVITRRGLINQDF
                     ADIESVMRDAGVAFLGIGIGKGEDRAQQASVRAMDSKLLERPVDNATAVILNISSNKN
                     ITMKEMNLAAAVIRQRCSETVDMKFGLILDDDLPEDEVRVTLIAAKFEDNEDLLSMDS
                     DIPAVYRMGLNEFLRGD"
     gene            9280..11016
                     /locus_tag="PKM49_00505"
     CDS             9280..11016
                     /locus_tag="PKM49_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNY81325.1"
                     /translation="MSTSIFQYKRLGEILIENQYLTEKEIELAVSLQKQEKKPLGQIL
                     VEKGFVTWNDIATALSKQYSLPFLESVAEIEPDPDALSILSKHNIENWRAIPFSKDED
                     RNLKVLIDQTNSLPSIKRDIQFITGYTPSFYIVSKPNLDYLLKELFGSAIDSVEDLKS
                     SIEVTSGKPQAVMELEPSAQMGDIDKESTADSAPVIKFVNEMVMGAVTQEASDIHIEP
                     QEKNVRVRFRIDGLLRKIAEYPVKLHSSIVSRIKIISKLDIAERRLPQDGKFFVRVKG
                     EQYDLRVSTMPSIFGEKIVLRLLRVSNSTKRLDDLGLSDYNYKRLSALLESPYGILLV
                     TGPTGSGKSTTLAGVLNQLKAVTNNMITVEDPVEYSITGTTQCQVQPEIGLTFARYLR
                     AILRQDPDIIMVGEIRDKETAILAMEASMTGHLVLSTLHTNTASAAIYRLVNMGIEAD
                     VLGVSLIGIVAQRLVRTLCDSCKKKAPLASEEMEKVATQLLPGHSEYSVCVPEGCQRC
                     RKTGYRGRTAIHEVLIMDKRIRALMLENTPERIIEENARKAGMRTMFEDGIYKALMGI
                     TSIDEVKRVAVE"
     gene            11009..12322
                     /locus_tag="PKM49_00510"
     CDS             11009..12322
                     /locus_tag="PKM49_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81326.1"
                     /translation="MNKPVEPPNRTSKDYVYHTKPPEVIREQLRERKERNPKGKRTRR
                     IVFWTVLFNLVILFAIFGIVFLSGQRGQKYEYIQNVTPFVLRISAAGEYVYGETPEII
                     IRISNSSSSLRECSIEKFEFYIKSETKTGKNVFTFVFPPKNTFLLDRYDSRAVYDFKR
                     DHPIFELQPGGYYIVCRFVVNHTPVELSKPFSINEVMSTTLFLRDDFWTPDIGDGAPE
                     SRIDAYIRIRNSAPKSLHYRFGAYNLKLTQSGSVVRSVKEDVSQKEVFLSSGESLDIP
                     LQPMRAPDLVGDYMLEWSFFIDDRLQEGQNRVYVRELSSTVAPEKLRILSYSVKAVAQ
                     GERYQSEILLANDLNDPLFLRIEGFAFYIYRDRSELYRYQAERALNALVPAHSTRTIF
                     RSEEWRELRFDRPGTYLIRVVLNTNQGEKVFEEEIFVLEKPVRTP"
     gene            12332..13831
                     /locus_tag="PKM49_00515"
     CDS             12332..13831
                     /locus_tag="PKM49_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY81327.1"
                     /translation="MKETDFKVARDPLLGEIRLSPLEMLIADIPSIQRLRSVSQLSGA
                     EKVYPGATHTRFLHSLGVMHIAGLYAEHLQLEPHKSRLLRLSGLFHDIGHGPYSHQFD
                     EVVYARAKIAGGHDDQRARILREVVPEQMLEAYRNRMKEYWRKEVQKDLRLSGFLESA
                     ASDTDEAILAAFAELCREVNALFEGEALGTPFFNIVQGPLGADRMDFVLRDAYYSGTS
                     QYGTVDLQRIIRNSSIVAVKGVERLCYDEKVIDNIYAVLFGRFMMYKNVYFHKTARAA
                     DLMLQKILELLYKPLGLEAIVHSPTEFVKWSEPYFLQQLWKLYHNRDTLSAENKTLVE
                     AAFQLWEDYSSRNLWKMIREVSFSIAGLDPGSIANSVGMETLSRLKSSIQKFLNQNGE
                     LQPEERRRFEHMLAAPEEVFHIDTPYKLSLAHPAEFFTNQVYIVSSTQENRKLIDFEE
                     FLKENPFYQTLSNSLLQIVRIYVDSADRDLLQKYRLVPKKEPLNLTTRW"
     gene            13840..15717
                     /locus_tag="PKM49_00520"
     CDS             13840..15717
                     /locus_tag="PKM49_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNY81328.1"
                     /translation="MKKRRITLWFIDTALILLSGLAALFIRFGWDFASIGRFTPAVLV
                     SSLVAICSLFLNGIYRIVWAYMTTREIGIVFRAFALGYLFVFVVDFSTDWVLPRSIGI
                     VMFTVGAILVITSRMWWSWYAHREYKTVREQASRRIAIIGAGESGVSLSEDLRRNPQS
                     GKVVLFIDDSERKIGREIRGIRVLGPISRVNDYVKEYRIDELLIAMPSAPSSQIQRVL
                     SSIDLLKVKVKILPNLLELIDQQPSSRLLREISIVDLLGRDQVAIDLGQIKSAFHNKT
                     VMVTGAGGSIGSEITRQVATMGIKHLLLLGRGENSIYEIDQEMTKKHPYLKIKRIIAD
                     ITDQARLEQVFETERPQFVFHAAAHKHVPLMEENPSEAFRVNSIGTKRLLDLSLRYEV
                     EGFLMISSDKAVKPSSIMGVSKRMAEMYIRAKAAESQSSKTRLTIVRFGNVLGSRGSI
                     IPKFERQIKAGGPVTITHPEMRRFFMTIEEASSLVLQTAAFKENGSLYVLDMGEMVYI
                     KDLVEKMISLAGYVPGQDIQIVYTGCREGEKLYEELFTDAEKPGPTAHPKIFRAREEE
                     PCSVEQVESWIFLLYERAKNNQKEALRQLIHQIIPENCLEEGEEYDAKRTERVSQTQI
                     R"
     gene            15674..16510
                     /locus_tag="PKM49_00525"
     CDS             15674..16510
                     /locus_tag="PKM49_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNY81329.1"
                     /translation="MLRELKEYLKPKSVEEAWAIYRDPVKSPALFLTGGLSVAQREDQ
                     TTKTLVDLSGLLGESITETPAAYILDGGVRIADCIKKIDIPFLQEALQTVGSRQIRNM
                     STIAGTLGQRYSWSDTLTALLALDATVDGYNGSAVSLPLERYLKGRAPLLITKVKIPK
                     RFHRGAFFKFSKVSYDIATLNLAVVFRIEREADPMENTVVQECRITCGARPGVAILLP
                     RTSEAVQGKTFSAVRRDLLPIQSIAALESEVSSSAEVTAEYRQQLVRTYVERGLRRLI
                     EK"
     gene            complement(16591..18321)
                     /gene="ade"
                     /locus_tag="PKM49_00530"
     CDS             complement(16591..18321)
                     /gene="ade"
                     /locus_tag="PKM49_00530"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HNY81330.1"
                     /translation="MRIIDIIPVARSEKPADVILKNCKVVNVFSGLIEETNVALFRKR
                     IAGVGDYTWGEKTIDLHGQYLVPGFINAHMHIESTMLEPREFAKAALSRGTTTVIADP
                     HEIANVIGLKGVEFFINYTEGIPMNIYFMLPSCVPATDYETNGSKVRVMDMIGFLEKY
                     PRVLGLGEVMNYPGILNRQEELLTMIELFRHKYKKIDGHAPLLRGKDLNAYISAFIRS
                     DHESTQPEEAMEKLSRGMQVLLRYGTGERDLENLVSIVNPVTQPFLSFCTDDKHPEDI
                     VKGDIDEMIRLVISRGVDPVTAIRIATINTARHYDLRSMGAIAPGYKADMVVVEDLQR
                     FSPKIVFKDSQIVAEDGVCVAKFPDPLVKIDTVMHTIFCPHYQAEDFGFNLKTERTMI
                     RSIEVTGVSVVTHERLTEVTVKEGEPDISEHDIAKIAVINRYTPEKQFSLGFISGTGI
                     KRGALALSVGHDSHNISVTGTSDSDMALAVNHVITCNGGMVVALDGEILACLPLPVAG
                     LMSDQPLKVVIAQNRRLLDAAKKTGCRLANPFMALSFVQLEVIPELKITNLGLMDTNT
                     FRFVDAVFPI"
     assembly_gap    18572..18671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19105..19893
                     /locus_tag="PKM49_00535"
     CDS             19105..19893
                     /locus_tag="PKM49_00535"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HNY81331.1"
                     /translation="MELRSWNRIRMYDLSQPISHLTPAWPTYEPLQIKFFKRLAPNGA
                     NGQLLTHSNHVGTHLDGSLHFCTHGRDIASIPLDELVGPGAIVDLSDIAEDYGIYTSK
                     DIEERVEVREGDILIINTGYHRYAFDQPEADEVRYMVKHPGPTMEFAQWCEKKRIKWI
                     GVDCGSADHPMNTKIRDWMPEKAAECEAFFQKKFGRSLDDLFPPDHYQLMHVRLFPQN
                     IIHAENLGGEIDLLLNKRTTIGCFPWRFEGGESCICRIVAFDEI"
     gene            19912..20796
                     /locus_tag="PKM49_00540"
     CDS             19912..20796
                     /locus_tag="PKM49_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNY81332.1"
                     /translation="MRAFDLFRPKDLYQAFDRLELNGHETMLMSGGTDLLIWIKKGLV
                     SPKEVVDLGSIDSLSHTELRGNDGVLIGTNVSLNRIISHPEIKRHCPALIEACEAHSD
                     AVVRNKATLVGNVCSAVPSGDTIPPLLCYEAEVIIQTSCSKRQLPLSQFLVGPRKKNL
                     SKTEIVTHLWIPFPKAELSVGVYRRASRRRALDLAQAAVACTVFLHQGRPEYRLAVGA
                     LSPVPVRIREGERVLNETYDIDKEKVERVVNSVQKAIRPITDVRGSREYRFAVTAELV
                     RESLSLCQTRIKTEGGGR"
     gene            20799..21299
                     /locus_tag="PKM49_00545"
     CDS             20799..21299
                     /locus_tag="PKM49_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY81333.1"
                     /translation="MPKRALSFVLNGERVEWEVEASDTLLRALRENGLTSVKRGCEEG
                     ECGACTVVIDDEAQKSCLVLALEVEGKSVLTTEGLPAAPGKLHPIQQAFVDEGAVQCG
                     FCTPGFIMTTYAFLKAHPNPTDEEIRHALSGNLCRCTGYLSIIKAVKAAAAALSNQSG
                     AAEGGR"
     gene            21302..23674
                     /locus_tag="PKM49_00550"
     CDS             21302..23674
                     /locus_tag="PKM49_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HNY81334.1"
                     /translation="MSEKRVFTDTGIGQRVERKFDREKVAGTLQYADDLPFGKDLLHA
                     RAVRSPIASGNIRSIDARKALAIPGVVAVITGKDFPYRFGLYLKDRTPIAIDRVRYVG
                     EPVALVVATSESIADRAAELIHVDYQELPAVFDMEKAANDTSILVHPDLEHYERVSFI
                     NPQPGTNIGNWFKIRRGDIEEAFAKSDYIVEERMECPQIAHGFLETHCCVAKQDMGTG
                     DITVWSSAQSVFAVREILAEGLGIDNNRIRVIAPPIGGGFGGKAGMTIEALCLSAAMN
                     PAVKGRPVKLFIPREEVMLSSWVRQAYIATIKMGITKEGKILGIKNAFYFDTGISAEY
                     GVNPVRSAGYTSTGTYYIPNVWTDSYAVYTNKPFGGAYRGFGLPELMSAMEVVVDIAA
                     KKIGMHPVEFRLKNLLRPGLPTCTGMPMSPHALDKIIEKARAAIRFDEKESPVREGFK
                     RGKGMALAIKAPAKPADAASSAIVKINADGTAEVQAATMDMGQGAYTAYAQMVSEELG
                     IPYDRIRLDYPDSATSPYDWQTVASRSCWAMGAAVKAAATDARQQLLALYAEYWQVEP
                     EDIVLKNGFVLCQKKNLSEPFGPKLQNGFPMPDEKLKGGPIIGRGRFVPADVVNPDAE
                     TGQSPKSAVHFTVGAIAIDVEVSESTGEVAVNRVVAGYDVGKAISPVNIQGQIEGGTV
                     QGISAALFEGLVFDEKGKLLNQDFTDYKIATIEEVDFPIDSFWEETPEEISPYGNRGV
                     GEHAMISPAPALNNAVYNAIGIRFHRYPLTRERVFMAARSKTETTEDWYE"
     gene            complement(23747..24328)
                     /locus_tag="PKM49_00555"
     CDS             complement(23747..24328)
                     /locus_tag="PKM49_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81335.1"
                     /translation="MKRYFAVFLFLSIVSIMGFSQIGTNLFGGFNYGLSTPASSPSVL
                     TDAASAIHTIDFGLLVDYEIAEGFAIGVGVGGAIPVTSGVKVKGEGGKDSDLKYFLDG
                     LLGISYAYPLKPVVLRADVLGGVSWFDLTRIGEFGFVVKAKFSLGFTVNNFTIYVGAG
                     YEMRQYNVKPAANETGKFTISSIPIELGVTIRF"
     gene            complement(24414..24860)
                     /locus_tag="PKM49_00560"
     CDS             complement(24414..24860)
                     /locus_tag="PKM49_00560"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HNY81336.1"
                     /translation="MPDKDRDIQEKEWMEEEDDNLCDCGCPHQHDEDDCCQCDEPMEG
                     EGDDLFVFSFRNEKDEEVYFALLDEFQHGEKEYWICQKVDVDQMEEPAFDEENDELYV
                     FIKEEEDGETVLSELESEEELETVVEEWQKAIEAESEKEEEASEEE"
     gene            complement(24873..25868)
                     /locus_tag="PKM49_00565"
     CDS             complement(24873..25868)
                     /locus_tag="PKM49_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81337.1"
                     /translation="MVETGKTTVREEVLTVKNLVKYFPIRGGVFKTIVGWVQAVDDIS
                     FHVYRGETFGLVGESGCGKTTVAMTLMRLYNPTSGSAYFGGKDILNIHGNELRNLKKD
                     IQMIFQDPYSSLNPRMRVKNIIAEGIKTHRLATSKQDLLDKVAEMMEKVGLSVDHMYR
                     FPHEFSGGQRQRIGIARALAVEPQLILCDEPVSALDVSIQAQVVNLLKDLQNHFGLTY
                     VFVAHDLAVIKHISDRIGVMYLGKMVEMTDKRNLFNHPMHPYTRALISAIPIPNPELK
                     KNRTILVGDVPSPINPPSGCRFHTRCPIAQAICKEQEPLFEEKSMGHFVACHYAK"
     gene            complement(25868..26881)
                     /locus_tag="PKM49_00570"
     CDS             complement(25868..26881)
                     /locus_tag="PKM49_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81338.1"
                     /translation="MPEKNILLEVKDLRTYFKTEDGIVKAVDGIDYVVYSGETLGIVG
                     ESGCGKSVNALSIMRLLDEKGQIASGEILMDGVDILKLSESAMRDIRGNDVAMIFQEP
                     MTALNPVFTVGDQIMEAIILHQGKKKEEAKQMAIEMLKKVGIPEPAKRVDEYPHQLSG
                     GMRQRAMIAMALSCNPKLLIADEPTTALDVTIQAQILELMLQLQKELGMAIIMITHDL
                     GVIAETCQRVAVMYAGKIVEYTDVRTLFKNPKHPYTWGLLNAIARLDIEQKELYNIPG
                     IVPDPLHFPKGCKFHTRCPFADSLCKAEEPEINEIEPNHHVRCFHIDRLEEAKLKSKA
                     GER"
     gene            complement(26892..27782)
                     /locus_tag="PKM49_00575"
     CDS             complement(26892..27782)
                     /locus_tag="PKM49_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81339.1"
                     /translation="MNTESRRVLRKLVKNPTAMLGIFLLIFFILVAIFAPVLAPPEPG
                     KDPFKMLPAKTAEGKMIWSSNPIAPDRAHPFGVIGKYDVWYGVIWGTRSAFRIGLIVS
                     IISSLIGIVVGSISAYFGGWIDEILMRITDMFMAIPFLVAAMIMTTILGKGLDKVTIA
                     LIIFGWMQVARLIRGNILQAKEEQYVLAAKALGIRDYSIIVKHLLPNTIFPVIVQISM
                     RMGSLVITAASLSFLGVGAEIGYADWGTLLNYARNYMLQLDKSWFAVVYPGIAMVLFV
                     LAWNLVGDALRDIFDPKMRA"
     gene            complement(27806..>28180)
                     /locus_tag="PKM49_00580"
     CDS             complement(27806..>28180)
                     /locus_tag="PKM49_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81340.1"
                     /translation="LWWAYILRITRSSMLEVMRKDYVRTARAKGLSEKVVINKHVKRN
                     ALIPVATVSGGMIMQLFAGTVIVETIFARPGLGRFTATSAVTLDYFSVIGAALFFSLI
                     LIVTNLLVDISYALIDPRIRLS"
BASE COUNT         6894 a         7243 c         7584 g         6359 t
ORIGIN      
        1 gctgccaggt ggtgtgatgg acactcacac acataaatcg agagcggtac atctccgact
       61 ccacggcgcc ttctcttgac actacttggt tacctcgcga cgaacctctt tgttatgtca
      121 ccccatacct tgacccacac acccaaaggt tttttgacga tcctctcctt ccacaatgat
      181 ttttctctcc gcttgacata tcagtgattt agtgttatac ttaggtagta cactatacta
      241 ctaatggggg gataccatga agaaccaacc gattgtgttc cgtttccttt gggtaggcgc
      301 catcgccctt gcggcgatct gcataccgat agtctcgatc cggctctttt tagcgctggc
      361 ggccggagta ctcttcgcgc tccaatttcg gaagctctcg ccgttgatca tcgttttctt
      421 ggtgatcgcc gcgctgatca tcgggatagg aaacgtcata cctcgatttc taccgttgcc
      481 ggtttgggga ttcggctcgg accttttccg gaatttcgct tacgaaaact cctatcggta
      541 caacccggag gttaagtctt cggattattc caagctgatc aaagccgata ccacggtaaa
      601 cggcgccccg gtggtgatcg tgagggttcc gggggtgaag atatccttcg acaaagattc
      661 cgacaaaatc tctttcccaa gccttctaaa agcgagaacc tccggttctc aattgacgtt
      721 cgaagcaggt aattttccac aagactcggc tgccgagatc gtcatcggga gtaaaacgcc
      781 cttggaccgc ctctcgctag acgtcagctt cagtgaaatc tcagcggatg aaggggtctt
      841 cacggtcaaa cggctggaaa tccgaacgaa ctacttgtcc ttcgacggcg cgctggtgtg
      901 cgatgacatc aacgtgacca cgaatgcgtt ggactggcgc ggatcgtttg aggcgaaaga
      961 ggcgaggatg caatcgaacg cggcgtcgat cttcgtgacg gttcggggta tggaaaacct
     1021 cgtgctcacg gcgcaggtgg tctacggaga gatcaagtat ctggaccgct gggaaggatc
     1081 tcggaaagcc cggattaccg gtaccttcgg atccgtcacg tttatgaaac ccaaggacag
     1141 cacgggaaaa ttggaactgc aaaactcttc gcagatggtc agtctgcaga ttcaagaata
     1201 ttgacgggag gttggaacga tgttgcccaa aattgaagcg ttctttatga aagagatgaa
     1261 agaaaaaacg ggaaggctgg cgattttatc actcttccta ttggttccct tttacccgtt
     1321 tcgtatattc ttcttgttcc tcctgtgcac ccatatgctg ggttgggatg tccaacacaa
     1381 acggtactcg tctttaatgg cgctgcccta tatgcccaaa cagttgttct ggctctccta
     1441 cctgtttctg gtcgtcatct ccgtagagac ccagttgatc ggcgcggcgc tgggcgcggg
     1501 tgtattcaat atgggctggg cccccctcgg gatacacttg ctcggctcat tgctcttcgt
     1561 cacgtcctat tacggaattt cgatgttggc ggtcaccttt gggctcgacc atttcgcgat
     1621 cccgcttctc gtgttcattg tcgacctgac gttgggtgga atcgggttca ccacgaacaa
     1681 cccctactcc ttgattagtc cggttcacca gaaatctctt ccgctcgcga taattccggc
     1741 ggtggccctg ctgctgggat ccgcctactt gttcgaaaaa aagggggcat ataaatgaac
     1801 gttcaaatca aagggttacg ccatgccttc ggcgcaaaaa cggttttaaa cggtatctcc
     1861 tttgaggtaa aagaagggga gatcttctcc ctcataggcc cgaacggggc gggcaagacg
     1921 accaccttgc ggtgtcttta cggcgatata ttccccgacg gcggatcgat cctgctgaac
     1981 ggtcaaaggg tttccagcaa gttgaaagag aagatcgcgg tcgtgacaga aagccgtttc
     2041 tcgttcagcc gcttcaccgg agaggattac atcaagctgt ggaaggcgct ttacccctct
     2101 tgggacgaaa aggtcttcgg aagcttcatc aatcattacc actttgagct ccaagcgcgc
     2161 gcggacacct attctatggg ttggaaaacc ttgctcaacc tcgcgttagc gttctcgagc
     2221 ggtgcggacc ttctcatatt ggacgagccc acgcaaaacc tcgatccttt gattcggcaa
     2281 gaggtgctca acattttgca cacgttcgtc gcgagggagg gaaagacgat cattatctcc
     2341 tctcatgaaa ttttcgagct cgaagagatc acctcctcct ttgcggtctt aagggaaggg
     2401 gacatcctat acaccgatca cctcgacgac gcgaaagaga accatcggat cgtcagtcag
     2461 tcggaaaagg tcagttacgg ggaggtaatc ggtctcgtca agaatgaaac cttgatacgg
     2521 acgaccgaag aggtggggcg ttatccaacg ttccaggaaa tcatcctggg gtatcttcaa
     2581 ggcaaaacgc gcttcttgcc tttttccagg tgatcatagg ggggattcta tgctccagcc
     2641 agtagagaaa cacagcggtg tcccggctta cgttcagatc atccatatga taaaaaaaga
     2701 aatcctgatg gggaatatga ccgaatcgag ccgacttcct ccggttcgcg agttggccgc
     2761 tatctttgag gtcaacatca atacggtcct gaaagctctg gataagatgt cccaagagga
     2821 gatcgtgcag tcggaacagg gggtcggtta cttcatccga aaaacccagg atgtgaatcg
     2881 ggaagcgatg ccggttttgg gagaggcgat ccgtaaactc aaacagtacg ggatcgggct
     2941 tgaaatgacg aaactcttgc tcgacgaact ttggcggaca gatccttgac ggccctgacg
     3001 cgcattatgc ttgattaagc cgaaccgggc gtttcatgac acttgcccaa ccggcgggag
     3061 gagaatgtca tcgccctccc gcctttctct cttttttggt gtgggtcaag ctttgggggt
     3121 gactaagagg ttcatcgcgg ggtaaccgaa gggaccccgc gatccccacc ctttgccatt
     3181 ttttcctctg tgttctctgt gccctctgtg cgaaccctga ttttgatctt tggaacgaat
     3241 gaaaaccagg gtatcgcgcc gtcgcgaaga gaaggggcgc ttacgccgcg atgcccgcgc
     3301 ggggttatcg cggggtaacc gaagggaccc cgcgatcccc cttttgttct ttttacaccg
     3361 cgatgatgtc gggggtatcg cgccgtcgcg aagagaagga gcgcttacgc cgcgatgccc
     3421 gtgtgggggt atcgcgccgt cgcgaagaga aggagcgctt acgccacgat gcccgtgtgg
     3481 ggtatcgcgc cgtcgcgaga agaaggggcg cttacgccgc gatgcccgtg tgggggtatc
     3541 gcgccgtcgc gagaaaaagg ggcgcttacg ccgcgatgcc cgcgttctct gtgttctctg
     3601 tgccctctgt gcgaaccttg tgttatcgag acaggccgag tggaagtcgg ccccactaaa
     3661 agaaggggcg cttacgtcgc gatgaccaca cggattagaa actgtgcgat tgtttctggc
     3721 tttgtttctt gattacaccc catagcttga ctcgcacttt atacacgaaa atgcaccgaa
     3781 atatggtaaa atagcagata tgaagacgat cggcgaggtc atcgacggac tttcgaggaa
     3841 aaaaggagtt ttttcggaga tcaaagtcag aaccatattc acgcgctggg aatcggtcgt
     3901 cggtccgttg ttggccgaaa agaccaaacc ggtttccttg agtcgtggag ttctgacggt
     3961 ctggtgtgcc gaaagcgttt gggcctcgga gctgaagttt tacacgaaag atatcctcgc
     4021 gaagatgaag gaacaactgc gagacgtccg ggatatccgt tcgattaaga tcgtctataa
     4081 ggagtgaaag ggttgtcgaa ggattacgga gctaaaaata tccaggtttt gaaggggctg
     4141 gaacccgttc ggaaaaggcc tgggatgtat atcggatcca ccggtaaggc cgggttgcac
     4201 catttggtca ccgaagtggt ggataacagc atagacgagg cgatcgccgg tgtgtgcaaa
     4261 aacatcgaga tcaccctttt tgaagacggg agtgtggagg tcaaggacga tgggaggggc
     4321 atcccggttg ggatccacga agaggagaaa aagagcaccc tcgaactcgt gatgaccgtc
     4381 ttacacgccg gaggaaagtt caacaaagag agctataaat tcagcagcgg tttacacggt
     4441 gtcggcgttt ccgttgtgaa cgccttgtcg gaatggcttc aggtttgggt gatgcaaggc
     4501 ggaaagctgt acacgcagcg ttatgcccgg ggggttcccc ttgataaggt gcaagaggtg
     4561 ggagaggcgg aaggacacgg aacgatcgtg cgattcaaac cggacccgac ggttttcacc
     4621 accacgcaat tcgaatttga ggtcttgaac aaccgcttta tggagcttgc ctttttgaat
     4681 ccgggactca ccataaaatt gaaggacctg cgggagaacg aagagatcaa atactgcttt
     4741 gaaggggggc tgacggagtt ctgcgcctac ctttccaaag gctataagaa tctccatccc
     4801 aaacccatct atctctccgg aaggtacaac gagatcgaag tggaaatcgc gctccaatac
     4861 atcctgagcg atgaggagat catgcggacc tatgtgaacg atgtcaacac gatcgaaggg
     4921 ggaacgcacc tcaccggttt ccgaaccgcg ttgacccggc tggtcaatga ctacatcaaa
     4981 aaattctctc tactgtcgaa cggaagacag caaaagacga aaaaagtcga gaaaaaaacc
     5041 gaagaggaag agcttgtcat acagggcgga gacatacgcg aaggcttggt ttctattctg
     5101 agtttgagcg ttcccgaacc gcagtttgag ggccagacca agtccaaact cggcaacgaa
     5161 gaggcgcaag aggcggtcgc cttcgtggtg cgagaacggt tgtccgagta cttcgatatg
     5221 tatagcgatg tggcgcgcct catcttagac cgcatcatac aagcggccaa ggcgcgactg
     5281 gccgcgaaaa aagcgcggga gatggtgaaa agaaagaacg ccctggagta ctcgaccctc
     5341 cccgggaaac tggcggactg ctcgacgaaa gaacgcgcga agtccgaaat tttcatcgtg
     5401 gaagggcctt cggcgggtgg ttctgcgaaa caggctcgcg atcgggaatt tcaagcgatc
     5461 ttgcccttgc gcgggaagat cctgaatgtc gaaaaagcct cgttagaaaa gttattgaaa
     5521 aacgaaacaa ttagtgatat tattacggca ataggaaccg gcatcgacga caccttcgat
     5581 atcgaacgca gcaggtacgg gaaagtgttc attatgaccg atgcggacgt cgacggcgca
     5641 cacatacgga ccctgctgtt gaccctattt ttccggcatt tccggccgat gatcaaagcc
     5701 ggtatgatct atattgcctt atccccgctt ttccgcgttg cgcacggaaa ggacgagtat
     5761 ttcgtctata ccgaagaaga gatgagggaa gtcacgaacc aattgagaga agcaggtaaa
     5821 aaaaacatct cggtgcaacg gtacaaaggg ctgggggaga tgaaccccga acagttgtgg
     5881 aagacaacga tggacccaca aaaacggagg atggcccggg tctctttgga agaccccgag
     5941 ttggcgaatt ccattttaat gacattgatg ggttcggatg tggaggaccg acgacgttac
     6001 atccagcatc acgcgttaca agtcaccaat ctcgatatat agtcggtgga gacccccttg
     6061 agactcaggg ccctatccgg tttgacgata ctgttggctt tgggggtatg cgtgatacta
     6121 tgttcgtggt tctatcgcaa gacctttgaa aagcgaaatg tcccgatcga gcgtctcctc
     6181 attctcaatg ccgtgggaga gccacacggg tcttcaggca taaataaccc cgcccggacg
     6241 agttcgagct ctttggagaa ccaactgagc cggtatgccg ggtttgggta ccttttctat
     6301 atggacggcc tgaatcgagt gttgaggccg ctggtcgggt atgagtgggc ggggcaggag
     6361 atttataacg ggaagataac actcctatta agggagcagc aggcggtttt tttatctgaa
     6421 accgaggacg gcgaaaggtg gctggtcaca gaccaggggc gtcggttttg gcgggtgcat
     6481 ccggaggacg agcgtatcta tgcggacctg cccgtctttt tcgggggcac gtggaaagag
     6541 caggagccgg tgatcgcgcg gttcctcacc tattcgatga cctttcgcaa ctatgtttcc
     6601 tcggtcagtg tcaccgagaa ggtgttgtac atccgaaacg gcgttctctt gatcgtgaac
     6661 caatgggaga acttagaccg cttgaatcat aacgaattca tacggacgat gagacaaaag
     6721 acagcctatg acttgtacag caatggttta ttctttccca taaaccgtaa atgatgaagg
     6781 agggaaaatg gcggaatcca cagaagtgtt aacggtcgtc gatatcggtt cgcatacgct
     6841 gaaaggagcg gtcgccggct ttgaaggcgg gagaacaact attttagcct attcgagaac
     6901 gaaagcgaga ggttttgagc aaggcgaact gaaagatatc gtcgcgctga gagaatcgct
     6961 ggaaaccctt ttaaaagact tgggaagtca attgtcgaaa agagtggatt cgtactttat
     7021 catctctttc gtggaaaaaa cgacgttttt aagcgaagag acccgtaact tagagatcag
     7081 cccccgtgaa ccggtcttga tcaacgaaga ccacgtttat gagctgttca cacgcatcgc
     7141 cagccaaacg gcccgaaaag atgagccgga agaaaactat cgtcttctcg acccacgtag
     7201 ggctgttcaa cacgtcatcc cccagaggta tctcctcgac gaaaccaaag gggtcctcaa
     7261 tcccatcgat atggaagcct ccgtcctggg catgaaggcc tccatcctct cgatagacgc
     7321 gacggtcaaa gactcgttga cgaatatttt ttccggcatc gcgggcgaaa aaccgaccgt
     7381 tttcattagc cccttcgtag cctctgaggt tatcttgaac ggaaaagaaa aggagcgcgg
     7441 ggtcgtttgt ctcgatatcg gccactcctt tctcaccgtc agcggctacc tcaacgggtc
     7501 ggtctttttc ctaaaaccgc tcgatcaagg gctcaggcac gtgatcaaag atatcgcgca
     7561 agtgttcaaa acctccttcg acgaagccga gcggatgctc atccaatacg ggaaaatcgc
     7621 aatcaaagat accagcaaag atacgatcac ctatacgctg ctcgacggaa agagcagcaa
     7681 acagatcaca cgggcgcagt tggcgatcgt gatttacgcg aaaacgaggg agatcatgaa
     7741 cgcggtgaag cgggagatgc gcgccatcac cttgcggatg gctgaagaag gggaaaaagg
     7801 cattcccggc ggactcgtga ttacgggagc gggctccaag atagacggaa tggttgagtt
     7861 cttgcgggac gccttcaagc tgccggttcg aatcggtcat gcaggcacgg gcggaactga
     7921 ctttcaactt ccagacgaac tgctggatcc gaccttttcg gcttgcgctg gaaatatcta
     7981 ttggttccac acgatcggtg gtttggaagc gctggaaggc cacgccctct cctcctcctt
     8041 gtcaacctcc gaaagcgagg gcaagccatc caggaaaaag aaaagccgag ccgttcgcag
     8101 gcccgcacga gaaaaagagg ggcccggtac ttggaataag attgcagaat tcttaaaaaa
     8161 actggtttaa tagagtatac gggaacaggg ataaacggag aacaggagga gggggttttc
     8221 ttggcttttg aaatagacga cgggaaggat atccgaagac caccgaaacg caacccgacc
     8281 atcaaggtct tcggtgtcgg aggagccggt aacaatgcag tgaatcgtat gatcgaaagt
     8341 gggattcacg gggtcatttt cgtagtggcg aatacggact tgcaggtgtt ggaggacgcc
     8401 aaggccgaca ttaaaatcca gctgggtaaa gagctgacga gagggttagg cgccggtggg
     8461 aatcccgatg tcggggaaaa ggcggcctac gaaagtaaag acgatatttt ggcggtcttg
     8521 gaagacaccg atatgctctt catcaccgcc ggactgggag ggggaacggg aaccggcgct
     8581 gcaccggtca tcgcgaaaat agccaaagaa atgggcatcc tgacgattgg caccgtcaca
     8641 acccctttct attttgaagg cgtcaaccgc tggaacaacg cgaaaaaggg tttggggaaa
     8701 ttgaaagaaa acgtggatac cttgatcaaa atttccaaca acaagctgct cgaggagttc
     8761 ccacaggata tcaccgttgt ggacgggttc ctgaaagcgg atgaggcgtt gcaccaaggg
     8821 atcaagggga tcagcgaggt cattacccgt agaggtctta tcaaccaaga ctttgccgat
     8881 atcgagtcgg tgatgagaga tgccggtgtc gcgttcttgg gcatcggtat cgggaaaggt
     8941 gaagaccgcg cgcaacaagc ctcggtcaga gcgatggaca gcaaactact ggaaaggccg
     9001 gtcgataacg cgacggctgt tattctcaac atatcgtcta acaagaacat cacgatgaaa
     9061 gagatgaacc tggctgccgc cgtcattcgg cagcggtgca gtgaaacggt cgatatgaag
     9121 tttggcttga tcttggacga tgatctgcca gaagacgagg tgagagtgac tctgatcgct
     9181 gccaaattcg aggataacga agacctcttg agtatggaca gcgatatccc tgcggtctac
     9241 cgcatgggat tgaacgagtt cctaaggggg gattgatcgt tgtctacctc aattttccaa
     9301 tataaacgtt taggcgaaat cctgatagaa aaccagtatc tcacagaaaa agagatcgag
     9361 ttggcggtca gcttacagaa gcaagagaag aaaccgctcg ggcagatttt ggtggagaaa
     9421 gggttcgtca cgtggaacga catcgctacg gcgctttcga aacagtactc gctccccttt
     9481 ctagagtcgg ttgccgaaat cgagccggat cccgatgcgc tcagcatcct cagtaagcac
     9541 aacatcgaaa actggcgggc gattccattt tcaaaagacg aagaccgcaa cctcaaagtc
     9601 ctgatcgatc aaacgaacag ccttccttcg atcaaaagag atatccagtt cataaccgga
     9661 tacacccctt ccttttatat cgtgtcgaaa ccgaacctgg attatttgct gaaggaactg
     9721 ttcggaagcg ctatcgattc cgttgaggat ttgaaaagct ccatcgaagt cacttcggga
     9781 aagccacagg ccgtgatgga attggagccc tccgcgcaaa tgggtgatat cgacaaagag
     9841 agcaccgccg attccgcgcc cgtgatcaaa ttcgtgaacg aaatggttat gggcgccgtc
     9901 acgcaagagg ctagcgatat ccatatcgaa cctcaggaaa aaaacgtgcg ggttcgcttc
     9961 agaatcgacg gattactccg gaagattgcg gagtaccccg tcaaactgca cagctcgatc
    10021 gtttcgagga ttaagattat ctccaagctg gacatcgcag aacgccggct tccgcaggat
    10081 ggcaagtttt tcgttcgggt gaaaggggag caatacgact tgcgggtctc gaccatgcct
    10141 tccattttcg gagaaaagat tgtgttgcgg ttattgcggg tctcgaattc taccaaacga
    10201 ctggacgacc tcgggttatc cgattacaat tacaaacgac tgtccgccct tttggagtct
    10261 ccgtatggga ttctgctcgt caccggtcca acggggagcg ggaaatcgac cactctcgca
    10321 ggggtattga atcagctcaa ggcggtgaca aacaatatga tcacagtcga agacccggtg
    10381 gagtacagta tcaccggcac cacgcaatgc caagtgcaac ccgagatcgg gttaaccttc
    10441 gcgcgttatc tacgcgctat tctacgccaa gaccccgata tcattatggt cggagaaatc
    10501 cgggataaag aaaccgcgat tcttgcgatg gaagcctcga tgaccggtca cttggttctc
    10561 agcacgctcc acacgaatac cgcttcggcc gcgatctata gactggtcaa catgggcatc
    10621 gaggccgacg tcttgggggt ttccctcatc ggtatcgtgg cgcaacgctt ggtgcgcacg
    10681 ctgtgcgatt cgtgcaaaaa aaaggcgccc ctggccagcg aagagatgga gaaggtcgcc
    10741 acacagctcc tcccaggtca ttcggaatac agcgtctgcg ttcccgaagg ctgtcagcgt
    10801 tgcagaaaga ccggttatcg cggtagaacg gcgatacatg aggttctcat aatggataag
    10861 agaatccgcg ctttgatgtt ggaaaatacc cccgaacgca tcattgaaga aaacgccagg
    10921 aaagccggaa tgcggacgat gtttgaagac ggaatttaca aagcgttgat gggaatcact
    10981 tcgatcgacg aagtgaagag ggttgcggtt gaataagccc gtcgaaccac caaatcggac
    11041 ctccaaagac tacgtttatc acacgaaacc gccggaggtc atccgcgagc agttgcggga
    11101 acgaaaagaa cgaaacccca aaggcaagcg aacgcgaaga atagtttttt ggacggtgct
    11161 tttcaacctc gtgatccttt tcgctatttt cgggatcgtt tttttatccg gacagcgcgg
    11221 gcagaagtac gagtatatcc agaacgtaac cccgtttgtt ttacgcattt ccgcggcggg
    11281 cgaatacgtc tacggcgaaa cgccggagat cattattcgc atttcgaaca gctcgtcgag
    11341 cctgcgggag tgttcgatag aaaaattcga attctatata aaatccgaga ccaaaaccgg
    11401 caagaacgtg ttcacgttcg tttttccgcc taaaaacacc ttcttgctgg atcgatacga
    11461 ttcgcgggcg gtctatgatt ttaagcgtga ccacccgatc ttcgagttgc agccgggcgg
    11521 ctattatatc gtgtgtcgct ttgtcgtgaa ccacacgccg gtcgaactct ccaagccttt
    11581 ttcgatcaat gaagtgatga gcaccacgct ctttttacga gatgatttct ggacccccga
    11641 catcggggac ggcgccccgg agtcccggat agacgcgtat atccgcatca ggaatagcgc
    11701 ccctaaaagc ctccattatc gatttggcgc ctacaacctg aaactcaccc aatcagggag
    11761 cgttgtgagg agcgtcaagg aggatgtgtc tcaaaaggag gttttcttgt catccggtga
    11821 gtcgctggat attccgttgc agccgatgcg cgcgccagac ctcgtcgggg attatatgct
    11881 cgaatggtcc tttttcatcg acgaccgctt gcaagaaggc caaaatcgag tgtacgtccg
    11941 agagctgtcg tcgaccgtgg ccccggagaa gttgcggatc ttgtcttatt ctgtcaaagc
    12001 cgtggcgcaa ggtgaacgct atcaatcgga aatactgttg gccaatgatc tcaacgatcc
    12061 ccttttctta cgtatagagg gcttcgcctt ctatatctat cgggaccgta gtgaactcta
    12121 tcgctatcag gcggaaagag ccctcaatgc gctcgtgccg gcccattcca ctcggaccat
    12181 ctttaggtcc gaagagtggc gggagttgcg atttgatagg ccggggacct acctcatccg
    12241 cgtggttcta aacacaaatc agggagagaa ggtctttgag gaagagatct tcgttctcga
    12301 aaagcccgtt cggacacctt aggaggaaac gatgaaagaa actgatttca aagtggcaag
    12361 ggatccgctt ttaggagaaa ttcgcctgtc gcctctagag atgctgatcg ccgatattcc
    12421 ctctatccaa cggctccgga gtgtttcaca gctgtcggga gccgagaaag tctaccctgg
    12481 cgcgacgcat acgcgcttct tgcactcgct gggcgtgatg catatcgcgg ggctgtacgc
    12541 cgaacacctg caactcgaac cgcacaaaag ccggctcctg cgtctgagcg gactgtttca
    12601 cgacatcgga cacggcccct atagtcacca atttgacgag gttgtctacg cccgagcgaa
    12661 gatcgcgggt ggtcacgatg atcagcgcgc gcgcatcttg cgagaggtcg tgcccgagca
    12721 gatgctggag gcctatcgaa accggatgaa ggagtattgg agaaaagagg ttcagaagga
    12781 cctccgcttg tccggttttc tcgaatccgc cgcgtcggat acggatgagg cgatcctggc
    12841 ggctttcgcg gagttgtgtc gagaagtcaa cgcgctcttc gagggagagg cgcttggcac
    12901 gcccttcttc aacattgtgc aaggcccttt gggagccgat cgtatggatt tcgtcctccg
    12961 agacgcttac tattctggca cctcgcaata cggcaccgtc gatttgcaga gaatcatccg
    13021 gaattcttcc atcgttgcag tcaaaggggt cgaacgtttg tgttacgacg agaaggtcat
    13081 cgacaacatc tacgcggtgc tctttggccg ttttatgatg tataaaaacg tctatttcca
    13141 taaaactgcc cgggctgccg acctgatgct ccagaaaatc ctggagttgc tctacaagcc
    13201 cctgggactg gaagccatcg tccactcgcc taccgagttc gtcaaatggt cggaaccgta
    13261 ctttctccag caattgtgga agctctatca caaccgagac accctttccg cggaaaacaa
    13321 aaccttagtc gaagcggcct tccaactttg ggaggactac tcttcacgga acctttggaa
    13381 gatgatccgg gaggtttcct tttcgatagc ggggttggat cccgggtcga tagcgaacag
    13441 cgtgggtatg gaaaccctct ctcgtctgaa atcgtcgata cagaagttcc tcaatcaaaa
    13501 cggagagctt cagcccgaag aacgccggcg tttcgagcat atgctcgccg ctccggaaga
    13561 ggtttttcat atcgacaccc cttacaagtt gagcttggcc caccccgcgg agttcttcac
    13621 aaatcaagtc tacatcgttt cgagcactca agagaataga aaattgatcg atttcgaaga
    13681 gttcctgaaa gaaaacccgt tctatcagac cttatcgaac agcctattac agatcgtccg
    13741 tatctacgtg gacagcgccg atcgagattt gttgcaaaag tatcgcttgg tgcccaaaaa
    13801 agaaccgctc aatctcacga cacggtggtg aggaatcttt tgaaaaaacg gcggatcact
    13861 ctgtggttta tcgataccgc actcatccta ttaagcggct tggccgccct ttttatccgt
    13921 ttcggatggg actttgcctc tatcggccgt tttacgcccg cggttctggt ctcctcgttg
    13981 gtcgccatct gttcgctgtt tctcaacggg atataccgca tcgtttgggc ctatatgacc
    14041 acccgcgaga tcgggatcgt ttttcgcgct ttcgcgttgg gttacctctt cgttttcgtg
    14101 gtcgattttt ccaccgattg ggttttaccg cggtcgatcg ggatcgtgat gtttacggtt
    14161 ggcgccatcc tggtcattac gtcgcgtatg tggtggagct ggtatgcgca tcgggagtat
    14221 aaaaccgttc gagaacaagc ctcacgtcgc atcgccatca tcggcgcggg cgaatcgggg
    14281 gtgtctctct cggaggactt gcgccgtaat ccccaatccg ggaaggtcgt cctgttcatc
    14341 gacgattcgg aacggaaaat tggaagggag atacggggca tacgggttct cggaccgatt
    14401 tctcgtgtga acgactacgt gaaagagtat cgcatcgacg aattgctgat cgcgatgccg
    14461 agcgcgcctt cttcgcagat acagagggta ttgtcttcta tcgaccttct caaagtcaaa
    14521 gtgaagatcc tgccgaatct gctggaattg atcgatcaac agccgagctc ccgtctgctc
    14581 cgggaaatat cgatcgtgga ccttttgggg agagaccagg tggcgatcga tttagggcag
    14641 ataaaatcgg cgttccataa caaaacggtg atggttacgg gagcgggggg cagcatcgga
    14701 agcgagatca cgagacaagt cgcaactatg gggatcaaac acctgctctt gttgggccgc
    14761 ggggagaaca gtatctacga gatcgatcag gagatgacga aaaaacaccc gtatctgaag
    14821 atcaagcgga ttatcgccga tatcaccgac caagcgcggt tggaacaggt cttcgaaacc
    14881 gaaaggccgc aattcgtctt tcacgccgcc gctcacaaac acgtgcccct tatggaagag
    14941 aacccgtccg aggcctttcg cgtcaattcg atcggtacga aaaggctgct ggatctatcg
    15001 cttcgctatg aagtggaagg cttcttgatg atctcttcag acaaggccgt gaaaccctcg
    15061 tctattatgg gcgtatccaa aagaatggcg gagatgtata tacgcgcgaa ggcggccgaa
    15121 agccaaagct ctaaaacgag gttgaccatc gttcgtttcg gaaatgtctt aggaagcagg
    15181 ggcagtatca tcccgaagtt cgagcggcag attaaagccg gggggcctgt tacgatcacg
    15241 caccccgaga tgcggcgctt ctttatgacg atagaggaag cctcttcgtt ggtcttgcag
    15301 accgctgcct tcaaagaaaa tggcagcctt tatgtgttgg atatgggcga gatggtctac
    15361 atcaaagacc tcgtcgaaaa gatgatctcg ctcgccggct acgtacccgg acaggacatc
    15421 cagatcgtct ataccggctg cagagaggga gaaaaactgt acgaagaact gttcaccgac
    15481 gctgagaaac ctgggcccac cgcccacccg aagatcttcc gtgcgcggga ggaagaaccc
    15541 tgctccgtag aacaagtgga atcttggatt ttcctattat acgaaagggc gaagaataat
    15601 cagaaagaag cgctacggca gctcatccat caaatcattc cggaaaattg ccttgaagaa
    15661 ggagaggagt acgatgctaa gagaactgaa agagtatctc aaacccaaat ccgttgaaga
    15721 ggcgtgggcg atctaccgag atcctgtgaa aagccccgct ttgttcttga ccggcggttt
    15781 gtctgtcgca caacgagaag accaaaccac gaaaaccctc gtcgacctga gcggactgtt
    15841 gggggaatcg attaccgaaa ctcccgccgc ctacattctg gacgggggcg tccggatcgc
    15901 cgattgtatc aaaaaaatcg atataccctt cctccaggaa gcgttgcaaa cggtgggatc
    15961 gcggcagata cggaatatgt ccaccatcgc ggggactctg ggacaacggt attcctggtc
    16021 ggacaccctc accgccttat tggcgcttga cgccacggtg gacgggtata acggaagcgc
    16081 ggtctccttg ccgctcgaaa gatacctgaa agggagagct ccgctcctca tcacgaaggt
    16141 gaaaatcccg aagcgatttc atcgaggggc cttctttaaa ttcagcaagg tgagttatga
    16201 catcgccaca ctcaatctcg cagtcgtttt taggatagaa agagaagcgg acccgatgga
    16261 aaacacggtg gtgcaagagt gccggatcac ctgcggggcg cggcctgggg ttgcgatcct
    16321 gttacctcga acgagcgagg ccgtgcaagg aaaaaccttt tcggccgttc gccgcgacct
    16381 cttaccgatc caaagcatcg ccgcgctcga atccgaagtc tcctcgagcg ccgaagtcac
    16441 ggccgagtac cgacaacagc tggtgcgcac ctatgtggaa cgtgggctcc gccgtctgat
    16501 cgagaagtaa cggaaaactt gccgagcaac cctgaatgaa aggcataggc cattcgagat
    16561 aagcgagaat tggtcttata ttaaccggtc ttagatagga aagaccgcat ccacgaatcg
    16621 gaaggtgttc gtgtccatca accccaagtt cgtgatcttc agttctggga tgacctccag
    16681 ttgtacgaaa gataaggcca taaacgggtt cgccagacgg cagcccgttt tttttgcggc
    16741 gtccagcagt cttcggtttt gcgcgatgac gactttgaga ggttgatccg acatcagtcc
    16801 cgccacagga aggggcaagc atgccaggat ctctccgtcc aacgccacaa ccataccgcc
    16861 gttacaggtg atgacgtgat tgaccgccag cgccatatcg ctatcagagg ttccggtgac
    16921 ggagatattg tgagaatcgt gcccaacgga tagagccaaa gctcctctct tgataccggt
    16981 accactgata aagcccaacg agaactgctt ttccggagta taccggttga tcaccgcgat
    17041 cttggcgatg tcgtgctccg agatatccgg ctcgccctct ttgaccgtga cttcggtcaa
    17101 cctctcgtgg gtcacgaccg agacgcccgt cacttcgata ctccgtatca tcgtacgctc
    17161 ggttttcaga ttgaagccaa aatcctccgc ctggtaatgc gggcaaaaga tggtatgcat
    17221 cacagtgtcg atcttgacga gcgggtctgg gaatttagcg acacaaacgc catcttccgc
    17281 gacgatctga gagtccttga atacgatctt aggggagaaa cgctgcaggt cttcaacgac
    17341 caccatatcg gccttatatc ccggcgcgat ggcgcccata ctccgcaggt cgtaatggcg
    17401 ggccgtgttg atcgtggcga tgcgtatagc cgtaaccgga tcgacacccc tcgagatcac
    17461 cagacgaatc atctcgtcga tgtctccctt cacgatatct tccgggtgct tgtcgtcggt
    17521 gcagaaagac aggaagggtt gtgtgactgg attgacgatg ctaaccaggt tttcgaggtc
    17581 tcgctcgcca gtgccgtagc gcaatagaac ctgcatccct cgggacagct tttccatcgc
    17641 ctcctccggt tgtgtgctct cgtggtctga gcggataaac gcggagatat acgcgttaag
    17701 gtctttacct cgcaggagcg gagcgtggcc atcgatcttc ttgtatttat gtcgaaacag
    17761 ctcgatcatc gtgagcagct cttcctggcg attgaggatg ccgggataat tcatcacctc
    17821 acccaagcct aaaacccgag ggtacttctc tagaaacccg atcatatcca tcacgcgcac
    17881 cttcgacccg ttcgtttcgt agtccgtggc cggtacgcaa gacggcagca tgaaatagat
    17941 gttcatcggt atgccttcgg tgtaattaat aaagaactcg acacctttca aaccgatcac
    18001 gttcgcgatc tcgtgaggat cggcgatcac cgtcgttgtc cctctggaaa gggccgcttt
    18061 cgcaaactcc cgaggttcga gcatcgtact ttcgatgtgc atatgggcgt tgatgaaacc
    18121 ggggacgaga tattgaccgt gcaggtcgat cgttttctcc ccccaagtgt agtcgcctac
    18181 accagctatt cttttacgaa agagcgcgac attcgtctct tctatcaggc cggaaaaaac
    18241 gttgacgacc ttgcaattct taaggataac gtccgcgggc ttttcgcttc tcgctaccgg
    18301 tatgatatcg ataattctca taactccccc ttttttaaaa aattgaccgc tcacggcctg
    18361 aatcattttg cccagttgga gtcggtcttg gcgaagatta tatcatacct gctaagcatt
    18421 ttcgcgtcga ccgtgagtat ccgactgggt gcgccctggg aacacctggg aacgcgagct
    18481 tttaagttgg cgtgtcttta aagaaaaaca gggttcgcac agagagcacg gagaggacaa
    18541 gcatcgcggc gtaagcgctt tttcttttcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nttgttccaa agataaaaaa acaatggttc gcacggaggg cacagagagc
    18721 acggaggaaa aactgggaag ggacagagct ctcgcggaaa aatagaagac atgccggact
    18781 aaagtccggc gttcccagaa aacagggttc gcacagagaa cacagagaac acggaggaaa
    18841 aactgggaag ggacagggct ctcgcggaaa taagaagaac ctgtcgactt aaaagtcggc
    18901 gttgcccgga aacccgtgcc gaactaaagc tcggcgttcg cagaggcgga tttttacgct
    18961 gcgaaccctc ccgacatctt ttcctccaaa aaatcaatga ccagtgactt gacaaacggc
    19021 gtggataaac ggtaaaatct ataattcaag cgattattta cgaaaggaat acaaggcgaa
    19081 aattcgcccg acggaggtaa gactatggaa ctacgcagtt ggaatcgtat ccgtatgtac
    19141 gacctttccc aaccgattag ccacttgacg cccgcttggc ctacttatga acctcttcaa
    19201 atcaagttct tcaagagact ggcgcccaat ggcgcgaacg gacaattact cacccactcc
    19261 aaccacgtgg ggacccactt ggatggttcc cttcatttct gcacccatgg gcgtgacatc
    19321 gcttccattc ctttggacga gcttgtcggt ccaggcgcga tcgtggacct ctccgacatc
    19381 gccgaggact acggcattta tacctccaaa gacatcgaag agcgtgtgga agtgagggaa
    19441 ggggatatac tcatcatcaa caccgggtat catcgatacg ccttcgacca gccggaggcc
    19501 gacgaagtcc gctatatggt caaacatccg gggcctacga tggagttcgc gcagtggtgc
    19561 gaaaagaaac ggatcaaatg gatcggcgtc gattgcggat cggcagatca cccgatgaat
    19621 acgaagatac gcgattggat gcccgaaaag gcggccgaat gtgaggcttt cttccagaaa
    19681 aagtttggtc gttccctcga cgacctcttt ccgcccgatc actatcaatt gatgcacgtg
    19741 cgccttttcc ctcaaaacat catccacgcg gagaacttgg gcggagagat cgaccttctc
    19801 ctgaacaaga gaacgacgat aggatgcttc ccctggcgtt ttgaaggtgg cgagtcgtgt
    19861 atctgtcgga tcgtggcctt tgacgaaatt taatcaagga gcgtgtttgg tatgagagct
    19921 ttcgaccttt tcagaccgaa agatctttat caggcattcg atcgtcttga gctcaacgga
    19981 cacgaaacga tgctcatgag cgggggcaca gacctgctga tctggataaa aaaagggttg
    20041 gtctctccta aggaggttgt agacctcggc tcgattgaca gcctaagcca taccgaactc
    20101 aggggcaacg atggggttct catcgggacc aacgtgtcct tgaatcggat catcagccat
    20161 cctgagatca agcgccattg ccccgcgttg atcgaggcgt gcgaggcgca ttccgacgcc
    20221 gtggttagga acaaagcgac tttggtgggc aacgtttgct ctgccgtccc ttccggggat
    20281 acgatcccgc ccttgctctg ctacgaagca gaagtgatca tccaaacctc ctgctcgaag
    20341 cggcaactcc cgctttcaca gtttttggtc ggcccgcgta aaaaaaacct ctctaaaaca
    20401 gaaatcgtca ctcatctttg gattcctttt ccaaaggccg agctgtccgt tggcgtttat
    20461 cgccgagctt ccagaagacg cgcgttggat ttggcccaag cggcggtcgc ctgcacggtt
    20521 tttttgcatc aaggacggcc ggagtaccga ttggcggtcg gcgcgttgtc tcccgtgccc
    20581 gtgcgtattc gcgagggtga gcgagtcctc aatgaaacct acgatatcga caaggagaag
    20641 gtcgagcggg tcgtgaactc tgtgcagaag gcgatccgac cgatcaccga cgttcgagga
    20701 agccgcgaat accggttcgc cgtcaccgcc gaattggttc gcgaaagcct ttctctgtgt
    20761 cagacgcgta ttaaaaccga aggaggaggt cgataattat gccgaaacgc gctttgtcct
    20821 ttgttttaaa cggcgaaaga gtagaatggg aggtggaggc gagcgacacg ctcctacgcg
    20881 cgttgagaga gaacggcctc accagcgtga aacgaggttg tgaggaaggc gaatgcggag
    20941 cctgcactgt ggtcatcgac gatgaggcgc aaaagtcctg cctcgtcctc gcattagaag
    21001 tggaaggaaa atctgtcctc acgacggagg ggttgcccgc cgcgcccggg aaactgcacc
    21061 cgatacaaca ggctttcgtt gacgaaggcg cggtgcagtg tgggttttgc acgcccggtt
    21121 tcattatgac cacctacgcc tttctgaaag cccatccaaa tcctacggac gaagagattc
    21181 ggcacgcttt gagcggcaac ctgtgccgat gcaccggtta cctcagtatt atcaaagcgg
    21241 tcaaggcggc cgcggcggcg ctctcaaacc aatcgggcgc ggcggaaggg gggagatgaa
    21301 gatgtcagaa aaacgtgtct tcacggatac aggaattggg cagcgagtcg aacgtaagtt
    21361 cgatcgcgaa aaggtcgccg gaactctaca gtacgccgat gaccttccgt tcgggaaaga
    21421 cttgttgcac gcgcgagcgg tcagaagccc tatcgccagc ggaaacatcc gttctattga
    21481 tgcgcgaaaa gccctcgcga ttccaggcgt tgtggcagta atcaccggga aagacttccc
    21541 ctaccgtttc ggcttatatt taaaagaccg gaccccaatc gcgatcgacc gcgtccgcta
    21601 cgtaggagaa ccggtcgcgc tggtagtcgc aacttccgaa tcaatcgcgg atagagcggc
    21661 cgaacttatt cacgtggact accaagaatt accagcagtt ttcgatatgg agaaagcggc
    21721 taacgacact tccattctgg tccacccgga tttggagcat tacgagaggg tgtctttcat
    21781 caacccgcag ccgggcacca acataggcaa ttggttcaag atacgtcgcg gagatatcga
    21841 ggaagccttc gctaaatccg attacatcgt ggaagagcgg atggaatgcc cacagatagc
    21901 ccacggtttc ttggaaacgc attgctgcgt cgcaaagcag gatatgggga ccggggatat
    21961 caccgtttgg agtagcgccc aatcggtttt cgcggtgcgt gagatactgg cggaagggtt
    22021 gggaattgat aacaaccgta tccgtgtaat cgcgccgccg atcggcggag gatttggcgg
    22081 gaaagcgggg atgacgatcg aagccttgtg cttaagcgcg gcgatgaatc cagcggtcaa
    22141 aggccgaccg gtcaagttat ttatcccgag ggaagaggta atgctttcct cctgggtaag
    22201 gcaagcctac atcgcgacga tcaaaatggg gatcacgaaa gaaggtaaga ttttgggcat
    22261 taagaacgcc ttctatttcg acaccggtat ttccgccgag tacggcgtca accccgtgcg
    22321 cagcgccgga tatacctcaa ccggcacata ctatatcccg aacgtctgga ccgacagtta
    22381 cgccgtctac acgaacaaac ctttcggggg agcttatcgt ggttttggct taccggaact
    22441 gatgagcgcg atggaagtcg tcgtcgatat cgccgcgaaa aagatcggga tgcatccggt
    22501 cgaatttagg ctaaaaaacc tccttcggcc gggattgccc acttgtacag gcatgccgat
    22561 gtccccgcac gcgttggaca agatcatcga aaaggcgcgc gcagcgatac gtttcgacga
    22621 aaaagagagt cccgttcgcg aagggttcaa gcgtgggaaa gggatggcct tagccatcaa
    22681 ggcgccagcg aaaccggcgg acgctgcatc gtccgcaatc gtcaagatca acgcggacgg
    22741 caccgcggag gtgcaggcgg ccacaatgga tatgggccag ggcgcctata ccgcctacgc
    22801 gcagatggtc tccgaagagc tgggcatccc ttacgatcga atcaggttgg attacccgga
    22861 ttcggccacc tctccgtacg actggcagac ggttgcgagc cgcagttgct gggcgatggg
    22921 cgcggcggtt aaagcggcgg cgaccgacgc ccgtcagcag ctactggcat tatacgcgga
    22981 gtattggcaa gttgaaccgg aagatatcgt tctgaaaaac gggttcgtcc tgtgccagaa
    23041 aaagaatctt tccgagcctt tcggcccgaa gttgcagaat gggttcccga tgccagacga
    23101 aaagctcaaa ggcggcccga tcatcgggcg cgggcgcttc gtgcccgcgg atgtcgttaa
    23161 tccggatgct gaaacgggcc aatcgccgaa aagcgccgtg catttcaccg tgggcgcaat
    23221 cgccatagac gtggaagtgt cggagagcac tggtgaagtc gcggtcaacc gcgtcgtcgc
    23281 gggatatgac gtaggaaaag cgatcagccc cgtcaacatc caagggcaga tcgagggtgg
    23341 gacggttcag ggtatctccg ccgccctatt cgaagggtta gtcttcgacg agaaagggaa
    23401 gctcttgaat caagacttca cggattacaa gatcgcgacg atcgaagagg tagacttccc
    23461 tatcgacagt ttttgggaag aaaccccgga agagatttct ccctatggaa accgaggagt
    23521 cggtgagcac gcgatgatct ctccggcgcc ggcattgaac aacgcagtct acaacgcgat
    23581 cggaatacgc ttccaccgtt acccgctcac gagggaacgg gtctttatgg cggcccgctc
    23641 taaaactgag acgaccgaag actggtacga ataatacccg tcagaagaag gattaggcaa
    23701 ccgaaaaaag cccttcgcgg gcttttttta ttgtattagg agtttcttag aaccggatgg
    23761 tcacacccaa ctcgatcgga atagaggaaa tcgtaaactt cccggtctcg ttagccgcgg
    23821 gttttacgtt atactgccgc atctcgtacc cggctccgac gtagatcgta aagttattga
    23881 cggtgaatcc gagactgaat ttggccttca ccacaaaacc gaattcgccg atccgtgtca
    23941 gatcgaacca ggaaacgcct cctaaaacat ccgccctcaa tacgacgggt ttaagcgggt
    24001 aggcgtaact gatgccgagc aatccatcca ggaaatactt cagatcagag tcctttccac
    24061 cttcaccttt tactttaacg ccagaagtaa cgggtatggc gccaccgact cccacgccga
    24121 tcgcaaaccc ttcggctatc tcataatcga ccagcaagcc gaagtctatg gtatggatgg
    24181 cggaggcagc atcggttaac acggaaggcg aggaagcggg cgtcgaaagc ccatagttga
    24241 atccgccgaa aagattcgtc ccgatttgag agaaacccat gatcgacaca atggataaaa
    24301 aaaggaaaac agcaaaatac cgtttcatta tgaacccccc catatctgtg catattcagg
    24361 tttattatac tctagaggag tctaaaaaag caaaaaatgg cgtaaaccgc tctttactcc
    24421 tcctccgacg cctcttcttc cttttctgat tccgcttcga tcgctttttg ccattcttcg
    24481 acgacagttt ccaactcctc ttccgactcg agttcgctca gcacagtctc gccgtcttct
    24541 tcttccttga tgaagacgta cagttcatcg ttctcttcgt cgaaggccgg ttcctccatc
    24601 tggtcgacat cgaccttctg gcatatccag tactctttct cgccgtgttg aaactcgtct
    24661 aatagcgcga agtagacctc ttcatctttt tcgttcctaa aagaaaagac aaagagatcg
    24721 tcgccttctc cttccattgg ctcatcacat tgacaacagt catcctcgtc gtgttggtgc
    24781 gggcaaccgc aatcgcagag gttgtcgtct tcttcctcca tccactcttt ttcctgaata
    24841 tctctgtctt tatccggcat aggttcctcc ttctattttg cgtaatgaca agcgacgaaa
    24901 tgtcccatcg atttctcttc aaataacggt tcctgttctt tgcatatcgc ctgcgctatc
    24961 ggacatcggg tatgaaagcg gcatcccgaa ggaggattga tagggcttgg cacatcgccc
    25021 accaagatcg tgcggttctt tttgagttcc gggttcggaa tcggaatcgc cgaaatcaga
    25081 gcgcgggtat agggatgcat cggatggttg aagaggtttc tcttgtcggt catctccacc
    25141 atctttccta gatacatcac gccgatacgg tcactgatat gtttgataac tgccaggtcg
    25201 tgggcgacaa aaacataggt aagcccgaag tgattttgaa ggtccttcag cagattcacg
    25261 acctgagctt gtattgaaac gtcgagcgcc gagacgggct cgtcgcacaa aatgagctga
    25321 ggttccacgg ccaacgcccg tgcgatcccg attctttggc gttgcccacc ggagaactca
    25381 tggggaaaac ggtacatatg gtcgacggat aacccgacct tttccatcat ctccgccact
    25441 ttatccagca aatcttgctt gctggttgcc aagcggtggg tcttgatgcc ttcggcgata
    25501 atgttcttca cgcgcattcg gggattcagg gagctatacg gatcctggaa gatcatctgt
    25561 atatcctttt taaggtttct aagctcgttt ccgtggatat tcaggatatc cttcccgccg
    25621 aagtaggcgg agccggaagt cgggttgtag agtcgcatga gcgtcatcgc cacagtggtt
    25681 ttcccacagc cgctctctcc aactaacccg aaggtttctc cccgatagac gtgaaagctg
    25741 atatcgtcta ccgcttgcac ccatccgacg atcgtcttaa aaacgccccc gcgaatggga
    25801 aaatacttga caaggttttt gacagtcagt acttcttcac gcacagtcgt tttccctgtc
    25861 tcaaccatta tcgctcacct gctttggact taagttttgc ctcttccaac ctgtcgatgt
    25921 ggaaacatcg gacgtggtgg ttgggttcga tttcgttgat ctccggctct tccgctttgc
    25981 aaaggctatc ggcgaagggg cagcgagtgt ggaacttgca ccccttcgga aagtgcagcg
    26041 gatcgggcac aatccctggg atattgtaga gctctttctg ttcgatatcc agtcgggcga
    26101 tcgcgttcag caagccccaa gtataggggt gtttggggtt cttaaagagc gttctgacgt
    26161 cggtgtactc cacgatcttg cccgcgtaca tcacggcgac gcgttggcaa gtctcggcga
    26221 tgacaccgag gtcgtgggta atcataatga tagccatccc cagctctttc tgcaactgaa
    26281 gcatcaactc cagaatctga gcttgtatcg tcacgtccag cgcggtggtg ggctcgtccg
    26341 ctatcaagag tttggggtta cacgacaggg ccatcgcgat catcgccctc tgacgcatcc
    26401 ctcccgacag ctgatgcggg tattcgtcga cgcgtttggc tggttcaggg ataccgacct
    26461 ttttcagcat ctcgatggcc atttgcttcg cctcttcctt tttcttcccc tggtgaagga
    26521 tgatcgcttc cattatttgg tcgccaactg taaatacggg attcaaagcg gtcatcggtt
    26581 cctggaagat catcgccacg tcgttgcctc ggatatctcg catcgcgctc tcactcaact
    26641 tgagaatgtc gacgccatcc ataagaatct ctccactggc gatctggcct ttttcgtcca
    26701 agagccgcat gatggataag gcgttcacgc tctttccgca cccggattct ccaacgattc
    26761 ccagggtctc tccagagtag acgacgtaat ctatcccatc aaccgccttc acgatcccgt
    26821 cttcggtctt gaagtaggtt ctcaggtctt tcacttccaa taagatgttt ttttctggca
    26881 ttctgtcacc ttcacgctct cattttcgga tcgaaaatat ctctcaacgc gtcgcctacc
    26941 aagttccacg ctaaaacgaa gagaaccatc gcgattccgg gatacacgac cgcaaaccag
    27001 gatttatcca gctgcagcat atagttcctc gcgtagttga gcagcgttcc ccaatccgcg
    27061 tatccgattt cggctccaac gccgaggaag cttaaagagg cggccgtgat gactaaggac
    27121 cccatccgca tcgagatctg cacgatgacg ggaaagatcg tgttcgggag gagatgtttg
    27181 acgataatcg aataatctcg aatacccaaa gctttcgcgg ccagcacata ctgttcttcc
    27241 ttcgcctgca ggatgttacc acgaatcaac cgggccacct gcatccaacc gaagatgatc
    27301 aaggcgatgg tgaccttgtc caagcctttt ccgagaatgg tcgtcataat catcgccgca
    27361 acgagaaacg gaatcgccat aaacatgtcc gtgatccgca tcaggatctc gtcgatccac
    27421 ccgccgaaat aagccgagat cgatccgacg acgataccga tcagcgatga gatgatcgaa
    27481 acgatcaagc caatcctaaa ggcgcttcgt gttccccaaa tgaccccgta ccatacgtcg
    27541 tatttcccga tgactccaaa cggatgcgcc cggtccgggg cgatcgggtt tgaactccaa
    27601 atcattttcc cttcggcggt cttcgcgggc aacatcttaa acgggtcttt cccaggctcc
    27661 ggtggcgcca gtacgggggc aaaaatcgca acaaggatga agaagatcag tagaaaaatc
    27721 ccgagcatcg ccgtggggtt tttcaccaac ttcctcagta ctcttctgct ctctgtgttc
    27781 atagccatat gttctgcact cccccctacg atagccttat ccgcgggtct atcaaagcat
    27841 aagatatgtc aacaagaagg ttcgttacga tcaagatgag gctgaaaaat aacgcggcac
    27901 cgatgaccga aaaataatcc agcgtgacgg ccgaggtggc ggtaaaccgt cccaagccgg
    27961 gccttgcgaa gatcgtttcg acgatgaccg tacctgcaaa tagctgcata atcatccctc
    28021 cggaaaccgt cgcgaccggg atcaaggcat tgcgtttgac gtgtttgttg atgacgacct
    28081 tttctgacag ccctttggct ctcgcggtcc ggacgtagtc tttgcgcatc acttcgagca
    28141 tactcgagcg cgtgatccgg agtatatacg cccaccaaag
//
LOCUS       DAOPXN010000004        26809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_1788_length_26809_cov_5.358787, whole genome shotgun sequence.
ACCESSION   DAOPXN010000004 DAOPXN010000000
VERSION     DAOPXN010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 26809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26809
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_26809_cov_5.358787"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PKM49_00585"
     CDS             <1..452
                     /locus_tag="PKM49_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81341.1"
                     /translation="KSYNLTNLEYAATYTWYVVALQSDGQQATSTERSFSTQAAQYAA
                     PEIALKSPGNRVGEQPTTVNLTWEATPGKQTNVGERAPTIEGYLVYFGVDGQKYGVPE
                     PVTATQTTKPGLAYGTTYKWQVVALQSNEKRATSTEWTFTTLAPVYG"
     gene            507..2048
                     /locus_tag="PKM49_00590"
     CDS             507..2048
                     /locus_tag="PKM49_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81342.1"
                     /translation="MRLEWVAAPGAQTIVGESAPYLLGFQVHYQKEGGAKVSDETGNQ
                     YYNLTDLDPEATYTWQVVAAQSDGQYATSTERTFKTGAGGTTGSIRRYSSSGDLKGKY
                     DKLSDAINEADNEDHIVVVGGTILNNETQQVTIDATWVTIYSSDPGNPFTIDMGGGGS
                     TPGSKRENSRVFHITNGASVTIRDAIIKGGDATDEEGGGIRITAGSTVTTINATITDN
                     KAGYYGGGVYIKGSTFNAYGTTITGNTAEAEGEWVRAYGGGVAVLSGTFNAYENTTIT
                     RNAAKVEGYVAEAYGGGVAVWEGVFNAYEGTTITGNTAEAEGDSTIAYGGGLCVGGDG
                     TINAYAGTTITGNTAEAEGDDAMAYGGGVEVWWGTFNATETTITENTAVSSHAFGGGV
                     DVSWGTFNAYENTTITKNAAEANGDSAEASGGGVVVGYHGTFNAQSVEISGNVAKAGG
                     GIFWKPNGVVRTNGQVWTPRTSKKDDFSVDTGGGIQSPCDTNDPVQVFENTADDGDST
                     QMKVE"
     gene            2921..4933
                     /locus_tag="PKM49_00595"
     CDS             2921..4933
                     /locus_tag="PKM49_00595"
                     /inference="COORDINATES: protein motif:HMM:NF005543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY81343.1"
                     /translation="MDRLPKRVVIVDGYSTGVFYMPLLKELGIEVIHVRSTPVSLNAH
                     ITDIANAALKKNATYFTALVDGARPFEEIVEEVSAYKPVAVIPGCETGVELADQLAHF
                     WGLPGNDLAFSRARRDKFAMYEAVSKAGLRHLDYGRFAASPPLVSWVKQKNRFPVVIK
                     PARSAGADGLHICRSLDEVEQAFEKLTGMNTIFGEPNTSVIAQEFADGYEVVVNTVSC
                     KGWHRVTDLWRYSKTITADGRSVYDGAELVPDFGEVTDEVLGYAFAVLDALKITIGAA
                     HTEIMVTADGPVLIECGARAMGGSFSQTLLRSCLGYTQLEVSLDAYLSPDTFRARWDR
                     PYALLRYALFKFLASTRKGMLDAIPGVTLLAGLPSVKGGNFLGSLESGEVERTVDLFT
                     SPADIYLCHEDRRVLHEDVSLIRDLEKEAQNLLFEMSPDHAQGHNPEWYLELPDDLWL
                     KPEEVGKPDADTIWKALGLREGVEALDCPCGDARVGIHLARRGVRITGIDVNPRFIQK
                     AKERFAAEGLPGDFSVGDMRDIRFEGRFEYIINWFNSFGYFDIETDFYVLKLFWKALR
                     PDGSLLIEAPNRSNIIANTRKIIQKDGHELARHWDDLTERLYAPAEVNVNGEKKTVWV
                     GSRMYSISQYRLLFRLARFSSVDVYDENLNPFSDEARRAIFIAHKT"
     gene            5041..6354
                     /locus_tag="PKM49_00600"
     CDS             5041..6354
                     /locus_tag="PKM49_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY81344.1"
                     /translation="MSIPTALGFLGQTIYDVVDMIWIGRVSSAAVAGITIFVIVFWTV
                     EVLNEIIGISSVSLISQSFGAKDYERTRRVIEQTLTFKALMALLAMIFLLIFLKPLLG
                     FLTQDEEVLKHALDYGFIRLFFLPMMFSSYTVNTALRCVGDAKSPMMIMLFVSILNAV
                     LDPIFIFERVPGTSLPGLNLGVFGAGLATVISISIAFAIGFYLLYSGKRGIKISLRGL
                     LKLDWSIDYRLLTIGLPTGFEMLIQKASWYLAMKVIAAFGTVAVATIGIGNRFTSLLG
                     MPLIGLYVGSTAIVGQNLGANRITTARETVKAGVVVGAIFVAIAAGLAFSFPEGIMRI
                     FIDDPAVVNLGIAMVRILAPGVLFLAVFYGIAAAFGGSGYMKPFITASVISRCCVLLP
                     LLAIFAFLLNWPVETIWVTLVLTDAASALVMFFAYCQRKWESKRV"
     gene            6768..7592
                     /locus_tag="PKM49_00605"
     CDS             6768..7592
                     /locus_tag="PKM49_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNY81345.1"
                     /translation="MKKTLFWLCLAILFSLSAFSAVRITVTIQPYYLILEELKTSNVE
                     VRRLIDASQNPHTFSPSVGQGKLLYDSGIILYNGLHLENYLEPTLESIKHQGKTVIAV
                     SDWISSAVNPHIWLDPVWLGEEIIPGLRELLLSVDPENRDHYLSESQRMIEEIERLHL
                     ELKAYLSAYTGKTVIMSHPSFAYFFERYGLKLDSIFEGEGDEPTAGKMKALIDGLKDR
                     QVIALFLEPQQPRRYVEILAKEGKIAVGTLDPLGWNAPSIIQLLRSNFEEIKRVFP"
     gene            7623..8366
                     /locus_tag="PKM49_00610"
     CDS             7623..8366
                     /locus_tag="PKM49_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81346.1"
                     /translation="MKSAPILEVSQISYQKHGHDILRDISFSLLPGEFAGIIGPNGAG
                     KSTLLRIIVGEIKQYTGTVRCSCKIGYVPQRKEFQRDFPIKAREVVAMGMYQRRGLSR
                     VFSQNDWEACKRLLDRVGIGELSEEPIGFFSGGEYQRLMLARAIAYEPDLLVLDEPEA
                     GVDEMGKVSFYELLTHWRKEQQRSILLVSHDIGLVLSACDTIMCLNKSLHCHKDAATV
                     STEDLQSVYTEDFDILIKGNRHFEKEHQR"
     gene            8363..9220
                     /locus_tag="PKM49_00615"
     CDS             8363..9220
                     /locus_tag="PKM49_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNY81347.1"
                     /translation="MMGFLSDLAQYHFLWLAFLTSLFVGLLSGTLSPIIVSRKIEYIS
                     DGVAHATFAGVAFSFLIGVSALPIAVLAALLFAFAVSVFSRRQRASENVMIGIFLPLF
                     MALGVLFVSLAKGYVPDMMGFLFGNILLISETDLWFLAIVWVTVTCFVALFFREIQFY
                     AFDEGMAKHYGVPVAFIHYVTLLCLSLSVVASVKIAGVILVNAFLITPGVTARFVAKS
                     FAQMIGYAVLLSLFACFFGLYLGYRLDAPPGTTMVFTLFGEFLLFYLLGLKKPTTRGD
                     RKSEQKSER"
     gene            complement(9275..11494)
                     /locus_tag="PKM49_00620"
     CDS             complement(9275..11494)
                     /locus_tag="PKM49_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HNY81348.1"
                     /translation="MENSVLKFLKRSDKWYIGGGKKLTWTPPFPSFLHHPGLWDNAGY
                     YDLRVDPGFTYDILENGNPYAWKQTKREWTPACWTCAYECDDLILEEEKTLTPDDFLG
                     IRVTLTNRTERPLTLQTVLWTAQKRNEDETLEDTQLIRRQTDHIVYKKRLVKGIRKPV
                     TVYAGLTLQNGRSANVSFSENTGNLPYWHLTPFYEKLTSDGLLNEENTDGITLNGLLY
                     MGIEAPHTLLPGQAKSFLCGMAMGSSAEAVENAMRRSLETDIVGVSAQNWESHFSSVP
                     LFSCTDPWLEKYYWYRWYGLRLFTLDTQEGKMRYPAIAEGLEYFRVLITYSAQCHMLE
                     TRWMREDAIAKGSLRNFIENQRTDGSFVGHIYLNGVQENGFYHADWGRALRETQAVHP
                     DIEYLKTVYEGLKRYATFFDEIRDRENSGLYDVVDQFETGQEYMSRYQAVDEMADRYG
                     WINNIRLKGIDATIYIYNLKKALAWAADKIGKGEERDRWKAGAEKIREAVLSEMWDEE
                     DEMFYDVNPRDFSRTKIKAAVCFYPYMTDIVSEKHLPGLKKHLLNEKEFWTPYPVPAT
                     AVGDLYFDPWAQWKGKRHNCPWNGRVWPMTNSHIADALGLSARRFSDQELRAKTVEFI
                     NKFVRMMFFEGDVERPNCFEHYNPFNGKASVYRGVDDYQHSWVVDLYFRYLIGLTIGE
                     TQIEVDPFPFDCAYDVKGVLIAGKRWDFSWDGQHYTISSEGKALHRDKQRRKMAFDK"
     gene            complement(11516..14596)
                     /locus_tag="PKM49_00625"
     CDS             complement(11516..14596)
                     /locus_tag="PKM49_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81349.1"
                     /translation="MINTTSRNPSILWIGEHSAETREAVHRARGQRPVVLYGKGLEIL
                     ARLGIDNAEAVKTEPIEKIRGPWDKRGFQSYQHHPIFNGLHGGFYSVLLDVLDPPYGE
                     VSLYCGKNAKVIAVEKRFIDYVRSRALIWEYPSEKILCVGGFLYPETRRHYPYETLVE
                     RFRANIEDYMLRASSEEYPCWPEITHGFFEKTASHKELVIEKAHRFPDATPLRISVPK
                     LRTGHYTNTSGARILVNAVDDGNIREVWAHPYRILKKLEIAVDGRPLSSLLINTVIEP
                     AYLLFETEEASVLVFASIDKPLLGIQFEARDSGVHRISLRFETDLRISWPMDDDYSGD
                     RRYTKDAQTGTLSFWTGDHRCACRMTVNRPAEIEATSDENLLTGALAVSMQHSLCLTL
                     CAAIEGEELIEGVSFQEELDRSVHYYADYLDRCVRIRADHSEWDRRIERAMIATLKFR
                     VSTPLLGTGLVAGYANSRSGWNRARPGYAWYFGRDSEWVSLAMLDWGDAETVRENLEL
                     LIRYQELTGKIYHELSTSGLVHYDAADATPLFLLTAIRYLKHTGNIEWFKRYKRNLLM
                     ALDYCASTDTDGDGCIENTIAGHGWIEGGKLYLSHASLYLNVIWHIVLKELVDLLPFI
                     EEDSVTFDKVSSLHLKVAHALPRFLNPERQRYGLGIDVNGERIDHFTVLPAVGIYLGS
                     LTGAAAQAHARDFSRYDYSTDWGVRIIGKSSGVYNPGGYHEGSVWPLFTGWASLSEFA
                     AGAALSGFTHVCASLKDSDYYAKGYIREVLHGELYQSSGVCLHQAWSESMAVQPWIEG
                     VVGFSPDALKKAVTLSPQIPPNLSRLTVSQLKCGDAEISLDYERSQEIGDAVTVHQRY
                     RVRSNRDLEIDFAPYIPKQAENIRVVVGQKRPKPEGVRVSSEKRFSYPSGYSVPDSPP
                     GIHYVKIPLPKILCSDEVTIEIDYQTPFEWMAIVPEFSEHGPSRFPRIIGWEKTNRSW
                     LLETEVPANVPTVIYGYLGKKYECKGSVYHEGALRCEIKGSTGEYYAHFIEIEG"
     gene            complement(14609..15457)
                     /locus_tag="PKM49_00630"
     CDS             complement(14609..15457)
                     /locus_tag="PKM49_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY81350.1"
                     /translation="MNRRNLDRSLSVITQIVIYALLFLLAASTLLPYILMIALSLMAE
                     FEMYQIPPKFIPTILRWSNYIEMWQAQPWGRYIFNTLFATVAVLAGQLLLVAMGGYAL
                     ARMKFPGRDFMFKLFIAFMMLPGVVTLIPGFIILHSLGWVDTFMALIVPSLGNIWGLF
                     LMRQYMLSLPSSLEDAGRIDGANRWQVFWKLILPLCKPVLATVGTFTFLSMWRSFFWP
                     LIVTRSKEMRVIEVGVAMFSSQYITNIPAQLAAATLSSIPMILVYLFAQKWIVQGIKM
                     TAGYDR"
     gene            complement(15475..17811)
                     /locus_tag="PKM49_00635"
     CDS             complement(15475..17811)
                     /locus_tag="PKM49_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81351.1"
                     /translation="MKTPVKMWIVCLVAVITIVSGFFAWRASASSAWMSTVTQNEKNT
                     LLYAENSLALAVEEANARLNELVENSVIDSPESVRAVVDRAALLFRDGFLYACFFGPD
                     GELVASKGAKVPEDFVLTVFYDTLFYSEGYALSEISYDTGTLHLQTAILLEDGSIVLV
                     GQDVLADFVRRLNAALGREYGFFYGDEERFAPFPFSSRSAVFFEEGFRDEYSRMFTTA
                     VDSQRPSAKLFSLRSGRSLVTSTALYDADHWDVKGFLVQVTPESFWKKGSDRLDWFVW
                     LGSLLSCFVAPFFLIRLHYRERTADRSPRLRNASLFYAIAAFFPIYLFFLYGAFLLAP
                     SLLREYESSSVLANGSLLQQDIEYRFDHIALSSKSIIEQMKRATRDDYRICPVDQTSR
                     FSTVTTLSSRQERLISPLAPADSSGAIEIGTLSIDRIGQTYVRFRSRELDWQVLHEDT
                     FVSNEILSVQFLGVILLLAFLAVALIFGFLTRHLHNRLLIRNTLFGYLFLAPALVHLI
                     WWAAGPLGFSLFLAFRRWSIVDPAKPFVGFDNFIELFQDGNFWNALKNTAVYSLYVPI
                     GMLFSLLLAMAVNKAGKIAVALRVLYYLPVVTAGVATTIVWKWIFNRDFGILNYILGW
                     FGIGKIAWIDSPQFALLSIMIISIWQAIGSQLLIFLAGLQGIPIDFYDAAKVDGAGKY
                     KAFRHITLPLLKPTTLFVLVTSIIGSFQVFTPVYVLTQGGPLRSTDVVFYHIWKAAWT
                     EMRMGYAAAQSWILFLLLMVLTYFEFKLYGKDSWQAYF"
     gene            complement(17946..19265)
                     /locus_tag="PKM49_00640"
     CDS             complement(17946..19265)
                     /locus_tag="PKM49_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNY81352.1"
                     /translation="MKNTLLKGNWIRVIIAVLLILALSTAAFGKTKLRVTTWAGAEEA
                     ALDESIIAEFMRLNPDYEVVYEAVASDNYYQKILTDIGAGTPPDVILLDAEMIPKFFE
                     GDFLTDLAPFLSRLAREGYGGSNVYEYFPVLVDIARIDRKIYSIPKDTSPMGVFFNKK
                     MFDEMKVPYPDEDGWTINQFIETAKALTKDLDGDGKNDTWGFAFPSWVGIAVPLLWAG
                     GGEVFSPDFSHTSGYLNSDINVKTYQTYLDLLKEGYAPTPQQASALGGNSALFYTGKV
                     GMILTGRWFNVSIKGQIAKGLDLQVGIASLPFADINKKDTVSYLSGWSVPSNVQDKRG
                     AVMLAAFLSSDYAQRKRCLEGSLAISANQAVAQLQGENDPLDAAFIKMLDYSRVPVGS
                     QTKFYRPKFEDLWAEAFDKINVGGESLRSVLDWMASEMDKAIAKGES"
     gene            complement(19262..20293)
                     /locus_tag="PKM49_00645"
     CDS             complement(19262..20293)
                     /locus_tag="PKM49_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY81353.1"
                     /translation="MRKPFITMADVAKKSGFSINTVSRALNNKPDIREETKARILQIA
                     QEIGFVKNRSASLLRSVETRIIGVIMADSSNPFYSEVLKGIEWASRKFDYQIILVNTE
                     RATKNEIRAVSLLMERRVDGLLIGPVQESRKHIEDLIHRNYPTVIVGRHFDQMEIDEI
                     YSDEINGGKLATQHLIDQGCRKILLLNGYSYKSPSHMRQEGYRQALEAAGIPYDPSYV
                     HISDILPEDGYRAILHTVCKGMPVDGIFCYNDIVAFGAMQALKDNHYRIPEDIAIVGY
                     DDIQYSGWVYPPLTTVSIQKHRMGFEAFRMLLNRLQGKRKKTQRKILPVELIVRKSSL
                     HLQKEGQRV"
     gene            complement(20571..21212)
                     /locus_tag="PKM49_00650"
     CDS             complement(20571..21212)
                     /locus_tag="PKM49_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNY81354.1"
                     /translation="MLPLAVYGAGGLGREILYYTHQYNEIHGKFVIEGFYDDGIEAGS
                     IVDGYPVLGGLETLKRKTAPTALVMGIADTKIKGRLMESLSDHPNISFPTIVHPTAFL
                     AHTAKLQEGVVLAPFTFVSLNAVVGRGVFVNVGTQLGHDAIIRDYCSLMPSVNLSGGV
                     VLEEGCYVGVGAKILQNITIGAWSVVGANSLVLRNVPSGVTAFGTPAIPRKMV"
     gene            22085..22492
                     /locus_tag="PKM49_00655"
     CDS             22085..22492
                     /locus_tag="PKM49_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNY81355.1"
                     /translation="MHLHHIGYLVPSIEGAIESFESLGFQKHSEIVKDEERQVFISFL
                     TDANHTLVELIQPVDERSPVFPLMVRQGPGTYHLCFSIPQSQKASALSRLKALRFVPA
                     GKIAPAPACENQEVGFFYSNKIGLIELLFISDT"
     gene            22506..22739
                     /locus_tag="PKM49_00660"
     CDS             22506..22739
                     /locus_tag="PKM49_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY81356.1"
                     /translation="MGPFEEFAIKIAEILEVDETQLTPETEFRAATPYWSSLMGFGIL
                     VMLEEDYGQVMSVDDFLKAKTVGELYQRTEPKG"
     gene            22746..22961
                     /locus_tag="PKM49_00665"
     CDS             22746..22961
                     /locus_tag="PKM49_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY81357.1"
                     /translation="MEERILQWLSTILRVTVTRDTTRQNTEQWDSLRHLQIIAGLEEE
                     FGIDVPLENVAEILSVPDILRYVEGTN"
     gene            22963..24027
                     /locus_tag="PKM49_00670"
     CDS             22963..24027
                     /locus_tag="PKM49_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HNY81358.1"
                     /translation="MATTITGFKVEGLCACVPKEEIDNREYGKALFGEDLEGIVQTIG
                     VSKRRVCPEGETTSLDLCVCAAKRLFERGDYDPGSFGGIVCVTQTPDLTLPNNATYAQ
                     HLLGLPQSTAAFDINLACSGYPYGLYVAGMMASTMRKRVLLLDGDTHSHFVSPHDKTT
                     ALLFGDAGSATVLAPRDVEGSETSFLFHTYGESRQALHIPDGGYRNRVNPHSGEYREY
                     ENGNRRRPIDMYMDGMAVFDFVAKRVPKALQQLLDEMGKTVRDLDWLVLHQANLFMIK
                     QVAKKIKMDLTKLPVCIHKYGNSSSTTVPLTIASELAASVREKANTILMSGFGAGLSI
                     GTALLSIGPCCCPGVVEYEP"
     gene            24017..24796
                     /locus_tag="PKM49_00675"
     CDS             24017..24796
                     /locus_tag="PKM49_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY81359.1"
                     /translation="MNPNQPLNVLIVGGSDGIGLETGALLCERGHCVTLVARDFEHKP
                     ERALRIIARLNQTYGDAQCRLIRQDVSRLEELESFSKGVKERVGPLDGFVYCAGAQKT
                     LPLAMTKPAAVQELFTLNTFAAMELVRCFSKVGFVRAGASFVLLSSLAPHESSPGKSA
                     YAASKAALEGFVASASTELMQKNIRLNAIAPGIVKTSMSEGFLSKMTVDQVRALEDTY
                     PLGFGEPEDVAWMIEFLLSPRSRWVSGKTFLIDGGHLPGGK"
     gene            24802..26469
                     /locus_tag="PKM49_00680"
     CDS             24802..26469
                     /locus_tag="PKM49_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNY81360.1"
                     /translation="MKKIALLSNVTVDSVRNRLEDKGIPGVYCAEGYGSWVEHLLPGG
                     KLFEQPLDGVIVLLDGSALLEQHGEENLPGFLENALALIRNSKEKLGKTVWILSTLDI
                     RQEKILPLTARRLDREASALWNQGVRELGGIVFDLEELVKEVGRERFYSRKLWYLGSI
                     PFSSAGEERIATGLARLWRAYQGKRKKVLAIDLDNTLWGGVVGEEGIDGITLSTEKEG
                     KAFHDFQKRILDLKRMGVMLTLVSKNNPEDALEVFRKHPAMVLQVEDFVSMKINWDPK
                     PQNIATLAKELNVGLDSFIMIDDSPFERQAVKEILPEVVAPDFPKDTSKLSDWIRELA
                     SEYFLFLETTEEDAQRTQMMRADISRKQVQQQFQDLDHYLSSLNMTIDIHLARDEDVP
                     RIAQLTQKTNQFNVTTRRYTEADILRMKEDPAYRLWIGSVEDRFGDYGKVVLLIARVE
                     DNEADYDTFLMSCRVMERHVEDAAISWIESQLGMEGVERVRAHYLPTPKNKPVEGLWQ
                     RLGYSTLDGADNFERDLRTPLPASAIRKAHVKIKGQKSAVDLSEENA"
BASE COUNT         6233 a         7115 c         7223 g         6238 t
ORIGIN      
        1 agaagtccta taacctgacg aacctggaat acgccgccac atacacgtgg tacgtcgttg
       61 ccctgcaatc ggacgggcaa caagcgacga gtaccgagag gtctttttct acacaggcgg
      121 ctcagtacgc ggccccggag atcgcgctca agagccctgg gaaccgcgtg ggggagcagc
      181 cgacgacggt gaacctgaca tgggaggcga caccgggcaa acaaacgaac gtgggagagc
      241 gagcgccgac gatagagggc tacctcgtct atttcggtgt ggatggccag aaatatgggg
      301 ttccagagcc ggttaccgcc actcagacga cgaagccggg attagcctat ggcaccacct
      361 acaagtggca ggtggtggcg ctgcaatcga acgagaagcg agcgacgagt accgagtgga
      421 cttttacgac gctggctcca gtgtatgggt gaccgacgat agagctcaag agccctgagg
      481 acaaagcgac ggacctggcg acgccggtga gactggaatg ggtagcggcg ccgggcgcgc
      541 aaacgatcgt aggcgaaagc gcgccatatt tattagggtt ccaggtccat tatcaaaaag
      601 agggtggtgc caaagtatcc gatgagacgg ggaatcaata ctataactta accgacctcg
      661 accccgaagc cacatacacg tggcaggtgg tggcggcgca atcggacggg caatacgcga
      721 cgagtaccga gcggacgttc aagacgggcg caggaggaac cacaggatct attagacgat
      781 atagctcttc aggagacctt aaaggaaaat atgacaaact cagcgacgcg atcaacgaag
      841 cggacaatga agaccatatc gttgtggtag gtggaacgat attgaataac gagactcaac
      901 aggtcaccat cgatgcgacg tgggtcacga tctactcttc ggatccaggt aatccgttta
      961 ctatagatat gggtggtggg ggaagcaccc caggttccaa acgagagaac agccgagttt
     1021 tccacatcac taacggcgcg tcggtgacca tccgcgatgc gatcatcaag ggcggtgatg
     1081 cgaccgatga agaaggcggc gggatacgca tcacggcagg tagtaccgtc acaaccatta
     1141 acgcgacgat tactgataat aaagcaggct attacggcgg cggtgtgtat atcaaaggaa
     1201 gcaccttcaa cgcctatggc acgacgatca cggggaacac cgcggaagcg gaaggcgaat
     1261 gggttagggc ctatggcgga ggcgtagctg tcttaagtgg cactttcaat gcttacgaaa
     1321 acacgacgat cacgaggaac gctgcgaaag tggaaggcta tgtggccgag gcctatggcg
     1381 gaggcgtagc tgtctgggag ggcgtattca acgcttacga aggtacgacg atcacgggga
     1441 acaccgcgga agcggaaggc gattctacca tagcttatgg cggaggcttg tgtgtcggcg
     1501 gtgacggcac catcaacgct tacgcaggca cgacgatcac ggggaacacc gcggaagcgg
     1561 aaggcgatga tgctatggct tatggcggag gtgtggaagt ttggtggggc actttcaacg
     1621 ccacagaaac gacgatcacg gagaacaccg cggtaagcag tcatgccttt ggcggaggcg
     1681 tggatgtttc gtggggcact ttcaatgctt acgaaaacac gacgatcacg aagaacgctg
     1741 cggaagcgaa tggcgatagt gccgaggcct ctggcggtgg cgtggttgtc ggctatcacg
     1801 gcaccttcaa cgcgcaaagc gtcgagatta gtgggaatgt tgcaaaggct ggcggtggga
     1861 ttttttggaa gcctaatgga gtggttagaa cgaacggtca ggtctggacg cctagaacaa
     1921 gcaaaaagga cgatttttcg gttgatacgg gtggaggcat ccaaagcccg tgtgacacta
     1981 atgaccctgt ccaagtcttc gagaatacgg cggatgatgg cgatagcacg cagatgaagg
     2041 tggaataata gaaaactttt gaagcacctt agaggcgtcg cacgtttgcg cgactccttt
     2101 ttttaagaac gctttgtcca ttgccagttt tttctccgtg tcctctgtgc gaaccgtgtt
     2161 ttaatctttg gaacgaacga aaaccggggt atcgcgccgt cgcgaagaga agaggcgctt
     2221 acgccgcgat gcccctccgt gtggggttat cgcggggtag ccagcgggct gacttcggaa
     2281 gcccaagaag tctcgcgatc tgcacttggg aacgccaaac tttagtttgg cacgtcttct
     2341 acgttttcat ctttttccct gtcctctgcg ccttctgtgc gaacttggtc atctgctttt
     2401 taatccaggc tttttttctt tgtgttctct ctgtgccctc cgtgttctct gtgcgaaccc
     2461 ttttttaaac tttggaacga acgaaaaccg gggtatcgcg ccgtcgcgaa gagaagaggc
     2521 gcttacgccg cgatgcccct ccgtgtgggg ttatcgcggg gtagccggcg ggctgacttc
     2581 ggaagcctac gaagtctcgc ggtccgctct gctgtctttc tatttcaaga cacgccaact
     2641 taaaagtcgg cattcccaat ttggtgtacc ccgatattat tcccacttgt ccccgacgat
     2701 atccgtccat atgtgttata atcgcggtag acttctgctt gcttacctga gccgatgatg
     2761 cgagccttat ccggagacag gtctctttcg ggtgataccc gcgtttatac cggtggttat
     2821 accgtcaaac aggcgaagcc gatctctttg accatagcct tgataagggt ctttaggtat
     2881 tttgtgtgat tttcagagta cggactcaaa ggaggaaaca atggatcgct tgccaaaacg
     2941 cgtggtgatc gtggatggat attccaccgg ggttttctat atgcccttgc tcaaggagct
     3001 cgggatagag gtcatccacg tccgttcgac tccggtttct ttgaacgcgc atatcaccga
     3061 tatcgccaac gcggctctca aaaaaaacgc gacgtatttc accgccctcg tcgacggggc
     3121 tcggccgttc gaagagatcg tcgaagaggt gagcgcctat aaaccggtgg ccgtcatccc
     3181 cggttgtgag acgggggtcg aactggcgga tcaattggcc cacttttggg ggctaccggg
     3241 caacgatttg gccttcagtc gggcccgccg agataaattc gctatgtacg aggccgtttc
     3301 caaagccgga ctccgtcacc tcgactatgg aaggttcgcc gcttcgcctc cgctggtctc
     3361 ttgggtaaaa cagaagaacc ggtttcccgt ggtcatcaag cctgccagaa gcgcgggagc
     3421 cgacgggctc catatctgcc gctctctcga cgaagtcgaa caagcgtttg aaaagctgac
     3481 cggcatgaat acgatctttg gagagccgaa tacgtccgtt atcgcgcagg aatttgcaga
     3541 tggatacgaa gtcgtcgtga acaccgtttc ctgcaaaggc tggcaccgtg tgaccgacct
     3601 ttggcgttac agcaaaacca taacggcgga cgggcgttcg gtctacgatg gtgccgaact
     3661 cgttccggac ttcggtgagg ttacggatga ggttcttgga tatgcgttcg cggttttaga
     3721 tgcgctgaaa attacgatag gagccgcgca taccgagatc atggtcaccg ccgacggtcc
     3781 cgtcttgatt gaatgcggcg cgcgcgcgat gggcggaagc ttttcgcaaa cgcttctccg
     3841 ctcctgtttg gggtatacgc aactcgaggt gagtctcgat gcctatctgt caccggacac
     3901 ctttcgagcg cgatgggatc gaccctacgc tttgctccgt tacgccctct tcaaattcct
     3961 cgcttcaacc cgaaaaggaa tgctcgacgc cataccgggc gtcacgctac tggcggggct
     4021 gccttccgtg aagggcggaa acttcctcgg tagcctggaa agcggggagg tggagcgcac
     4081 cgtggacctg tttacctccc cagcggatat ctacctctgt cacgaagaca gacgcgtgtt
     4141 gcacgaggac gtttcgttaa tcagggattt agaaaaagaa gcgcagaacc ttttattcga
     4201 aatgtctccc gatcacgcgc aagggcacaa ccccgagtgg tacttggaac tacccgacga
     4261 tctatggctg aaaccggagg aagtgggtaa acccgacgcc gataccattt ggaaggccct
     4321 aggcctacgc gagggtgtgg aagcgctcga ttgcccgtgc ggggacgcgc gcgtgggtat
     4381 ccacctggcc cgacgcgggg ttcggatcac cggtatcgat gtgaaccccc gttttatcca
     4441 aaaagcgaag gaaaggttcg ccgccgaagg gttgcctggc gatttttccg tcggggatat
     4501 gagggacatt cgcttcgaag ggcgcttcga atacatcatc aactggttta acagcttcgg
     4561 atacttcgat atcgaaacgg acttctacgt tctcaaattg ttttggaagg cgcttcgccc
     4621 cgacgggtca ctgttgattg aagcgcctaa ccggagcaat atcatcgcca acacgagaaa
     4681 aattatacag aaagacggcc acgagctggc gcgccattgg gatgacctga ccgaacggtt
     4741 gtacgccccg gcggaagtga acgttaacgg cgaaaagaag accgtctggg tcggtagccg
     4801 tatgtattcg atttcccaat accggttgct gttccgactc gcccggttct cgtccgtcga
     4861 cgtctacgac gaaaacctga acccatttag cgacgaggcc cggcgggcca ttttcatcgc
     4921 ccataaaacg tagtctttaa cgtagaaaaa gggcttgata gtatccgaaa aaagaaggaa
     4981 gtcaacgcaa tagaaaaacg cgatttaacg actggcggtg tctacagaaa cctctttgcg
     5041 atgtccatac cgaccgcttt ggggtttttg ggccaaacca tctacgacgt ggtggatatg
     5101 atatggatcg gacgggtatc cagcgccgcc gtcgccggta tcaccatctt cgtcatcgtt
     5161 ttttggacgg tggaagtcct gaacgaaatc atcgggatca gttccgtttc gttgatctcg
     5221 caaagcttcg gagcgaagga ctacgagcgc acccgacgcg tgatcgaaca gactttgacc
     5281 ttcaaggcgc tgatggctct gctggccatg atattcctgt taatcttcct aaagcctttg
     5341 ttgggctttc tcacacagga cgaagaggtg ttgaaacacg ctttggatta cggattcatc
     5401 cggctcttct tcctaccgat gatgttctct tcgtatacgg tcaatacggc gttgcgctgc
     5461 gtaggcgacg ccaagagccc gatgatgatt atgctcttcg tctctatcct caacgcggtc
     5521 ctcgacccga ttttcatttt tgagcgcgtt ccgggcacat cgctcccggg cttgaacctc
     5581 ggtgtctttg gcgcggggct cgcaacggtg atctcgatct ccatcgcgtt tgcgatcggc
     5641 ttctacttgc tctattccgg aaaacgggga atcaagatat cccttagggg gctgttgaag
     5701 ctcgactggt cgatcgacta tcgactactc acgataggcc tgcccacggg cttcgaaatg
     5761 ctcatccaga aggcctcctg gtacctcgcg atgaaagtca tcgcggcttt cggcacggta
     5821 gccgtcgcca caatcggcat cggcaaccgg tttacaagtt tattgggaat gcctttgatc
     5881 ggcttgtacg tcggaagcac ggccatcgtc gggcaaaacc tgggcgctaa tcgaatcact
     5941 acagctcgag aaaccgttaa ggccggggtc gtcgtcggcg cgattttcgt cgctatcgcc
     6001 gctggcctgg ccttctcgtt cccagaaggg atcatgcgta tctttatcga tgatccggcg
     6061 gttgtcaacc taggcatcgc gatggtacgc atcctcgcgc cgggcgtttt atttcttgcc
     6121 gtgttttacg ggattgccgc cgctttcggg ggctcggggt acatgaagcc gtttattaca
     6181 gccagcgtga tcagccgctg ctgcgtgctg ttgccgttac tcgcaatttt tgcctttctt
     6241 ttaaactggc ccgtcgagac catctgggtc accctggtac tgaccgatgc cgcctccgcg
     6301 ctcgtgatgt tcttcgccta ttgccagaga aaatgggagt ctaaaagagt gtgaagggcg
     6361 tttcacctcg gagccgcgct ctcacgatcg gacctatcgc ctaagcgccg ttttctttct
     6421 gcccaccact tgtgactcct cttgacttaa ccgaagtgaa actgttaaaa tcaccgcgaa
     6481 tgcttggcgc aaaccatttc ttgcatcacc ttgtggagaa cgggaaggcg cgctcgctat
     6541 cctttcggac gtcggctcga ttctacgccc cttatcgcga gtatccgccc attattcatt
     6601 caacaaacgc tgtcgacctg cccagtcgtt cttgaggggg ataagaggca agcctatcaa
     6661 accctaaggc gggccggagg ggcgacatgt ggcttttatc aagcgatccc gtcaacggct
     6721 ccacaactgt ggaagacgca aaggccctta caataggagg taaagggatg aagaaaaccc
     6781 tattctggtt gtgtttggcc atcctctttt ctctttccgc cttctccgcc gtgcggatca
     6841 ccgtcaccat acaaccttat tacttgattt tggaagaact gaaaacgtcg aacgtggaag
     6901 tgcgcaggct catcgacgcg tctcagaatc cccatacttt ctctccctcc gtcggtcagg
     6961 gaaagttgct ctacgattcg ggcattattc tgtataatgg gttgcatctc gagaattacc
     7021 ttgaaccaac cctcgagtcc ataaaacacc aagggaaaac ggtgatcgcg gtgagcgact
     7081 ggatctcctc agccgtcaat ccccatatct ggctggatcc ggtatggtta ggcgaggaga
     7141 ttatccccgg tttgagggag ctgctcctct ccgtcgaccc cgaaaaccgt gatcattacc
     7201 tatcagaaag tcagcggatg atcgaagaga tcgaacggtt gcatctcgaa ctgaaggcct
     7261 atctttcggc gtatacagga aaaactgtga tcatgtcgca cccgagtttc gcctattttt
     7321 tcgaacgata cggtttgaaa ctcgactcca tcttcgaagg agagggggac gagcccacgg
     7381 cgggtaagat gaaggctttg atcgacggtc ttaaggatag acaagtcatc gcgttgtttt
     7441 tggaaccgca gcagccccgg cggtacgtcg aaatcttggc gaaggaaggg aagatcgcgg
     7501 taggaacctt ggatccgctc gggtggaacg ccccttccat cattcagctg ctcaggtcga
     7561 atttcgaaga aataaaacgg gttttccctt gagcgggggt cttggccatt ggagggtata
     7621 gaatgaaatc ggcgccgatc ttggaagtct ctcaaatctc ttatcaaaaa cacggacacg
     7681 acatactccg ggacatctcc ttttcgttgc taccggggga gtttgccggg atcatcgggc
     7741 cgaatggggc gggaaagtcc acgctcttgc ggatcatcgt cggcgagatc aagcagtaca
     7801 cggggacggt tcgatgttcg tgtaagatcg ggtacgtgcc acaaagaaag gagtttcagc
     7861 gcgactttcc gatcaaggcg cgggaagtcg ttgcgatggg gatgtatcag cggcggggac
     7921 tctcgcgcgt tttttcacaa aatgattggg aagcgtgtaa gcgtctgtta gaccgtgtcg
     7981 gaatcggcga gttgtcggag gagccgatcg gctttttttc cggcggggaa taccagcgcc
     8041 tgatgctggc gagggcgatc gcctatgaac ccgacctatt ggtcttggat gagccagaag
     8101 ccggggtgga cgagatgggg aaggtctcct tttacgagct gctgacgcat tggcggaaag
     8161 aacagcagcg ctcgatcttg ctggtcagtc acgacatcgg gctggtcttg agcgcttgtg
     8221 acacgatcat gtgcctgaat aagtcattgc attgccataa agacgcggcc accgtatcta
     8281 ccgaagacct gcaaagcgtt tacaccgaag acttcgatat cctgatcaaa gggaatcgcc
     8341 atttcgaaaa ggagcatcag cgatgatggg gtttctaagc gacctcgcgc agtaccactt
     8401 tctgtggctc gcctttctga cctctctctt tgtcggcctt ctgagcggga ctctttcacc
     8461 gatcatcgtc agtcggaaga tcgaatacat cagcgatggc gtcgcgcacg ctaccttcgc
     8521 tggcgtcgct ttctcgtttt tgatcggcgt gagcgcgttg cccatcgcgg ttctggccgc
     8581 tctgctgttt gccttcgcgg tcagcgtgtt cagccgaagg cagagggcat ccgaaaacgt
     8641 gatgatcggt atcttcctcc cgctctttat ggcgttaggc gttctattcg tctcgctcgc
     8701 gaaggggtac gtcccggata tgatgggctt cttgttcgga aatatcctct tgatttccga
     8761 aacggatctc tggtttttgg cgatcgtgtg ggtcacggtc acctgttttg tcgcgctgtt
     8821 ttttcgggaa atacagtttt acgcctttga cgaagggatg gccaagcact atggcgtccc
     8881 tgtcgctttc atccattacg tcacgctgct atgcctttcg cttagcgtgg tcgcttccgt
     8941 gaagatcgcc ggcgtcatcc tcgtgaacgc gttcctgatc acgcccggag tgacggcccg
     9001 attcgtcgca aaatctttcg cccagatgat cggctacgcg gttctgctca gcctatttgc
     9061 gtgcttcttc ggtttgtact tgggataccg gttggatgcg ccccccggga cgaccatggt
     9121 tttcacgctg ttcggagagt ttctattgtt ttacctcctc ggattgaaaa agcccaccac
     9181 gcgcggcgat cggaaaagcg aacagaagtc ggaacgttag cgacgcgagt tgttagagat
     9241 cgttcacgcc catcaaaaca ggcgtcaggc gctctcactt gtcgaatgcc attttacgac
     9301 gttgcttgtc tcgatggagc gctttaccct ctgatgaaat cgtgtagtgt tggccatccc
     9361 aagagaagtc ccagcgttta ccggcgatga ggaccccctt gacgtcataa gcgcaatcga
     9421 aggggaaagg gtcgacctcg atttgcgtct ccccgatcgt caagccgatc aggtatcgga
     9481 agtagagatc caccacccaa gagtgttgat aatcgtctac gccgcgatac accgaggctt
     9541 tcccgttaaa agggttatag tgctcgaaac agttcgggcg ctcgacgtcg ccttcaaaaa
     9601 acatcatccg aacgaactta ttgatgaatt cgacggtttt cgcccgtagt tcctgatccg
     9661 aaaagcgtct ggcggagagt cccaaggcgt ccgcgatgtg gctgttcgtc ataggccaca
     9721 ccctgccgtt ccaggggcag ttgtgtcgtt ttcctttcca ttgcgcccac gggtcgaagt
     9781 ataggtctcc taccgccgtg gccggtacgg ggtaaggcgt ccagaattcc ttctcgttga
     9841 gcaagtgctt ttttaagccg ggcaggtgtt tttcagaaac gatatcggtc atataggggt
     9901 agaagcagac ggcggctttg atcttcgtcc gggagaaatc ccgcgggttc acgtcgtaaa
     9961 acatctcgtc ttcttcatcc cacatctcgc tcagaaccgc ctctcgtatc ttttccgctc
    10021 ccgctttcca tcgatcgcgc tcttcgccct ttccgatctt atcggccgcc cacgccagcg
    10081 ccttcttcaa gttgtagata tagatcgtgg cgtctattcc cttcagacgg atgttgttga
    10141 tccacccgta tcggtccgcc atctcatcca cagcctgata ccggctcata tactcctgac
    10201 cggtttcaaa ctgatcgacc acgtcgtaaa gtccgctgtt ttcgcggtct cttatctcgt
    10261 cgaaaaacgt ggcgtaccgc ttcaacccct catacacagt tttcaggtac tcgatgtcgg
    10321 gatgcaccgc ttgcgtttcc cgaagggcgc gcccccagtc cgcatggtag aacccattct
    10381 cctgcacacc gttcaaatag atatgcccaa cgaaagaccc gtccgtgcgc tggttttcta
    10441 tgaaattcct caaactccct ttcgcgatcg cgtcctcgcg catccagcga gtttctagca
    10501 tatgacactg cgcgctgtag gttattaaca cacggaagta ctccaatcct tccgctatgg
    10561 cagggtagcg catttttcct tcttgcgtgt ctaaggtgaa taggcgcaat ccgtaccaac
    10621 ggtaccaata gtacttctct aaccaggggt cggtacaaga aaagagcggc accgacgaaa
    10681 aatgggactc ccaattttgc gccgaaactc cgacgatgtc cgtctccaac gaccgccgca
    10741 tcgcgttttc taccgcttcg gcgctactgc ccatagccat tccgcagaga aaagatttcg
    10801 cttggccggg taagagtgtg tggggcgctt cgatacccat atacagcaac ccgtttaacg
    10861 tgatgccgtc tgtgttctct tcgttcaaca gcccatcgga cgtcaatttc tcgtagaagg
    10921 gcgtgaggtg ccaataggga aggtttcccg tattctctga aaaagagaca ttggcgctgc
    10981 gcccgttttg aagggtcagc ccggcataga ccgtcaccgg tttacgaatt ccttttacca
    11041 aacgcttttt atacacgata tgatcggtct gtcggcgtat cagttgcgtg tcttccagtg
    11101 tctcgtcttc atttcgtttt tgcgcggtcc aaaggacggt ttggagtgtt agcggtcttt
    11161 ccgtccgatt cgtcagcgtg acccgaatgc ccaagaaatc gtctggagtg agcgtctttt
    11221 cttcctccag gatcaaatcg tcgcactcgt aggcgcaagt ccagcaagcg ggggtccatt
    11281 cccgtttcgt ctgtttccaa gcgtagggat ttccgttttc taaaatatcg taggtgaacc
    11341 cggggtccac acgcaggtca tagtacccgg cgttatccca gagtccggga tggtgtaaaa
    11401 aactcgggaa aggcggagtc caggtgagct tttttccacc gcctatatac catttatcgg
    11461 agcgttttaa gaatttcaac acagaatttt ccatcgtttc cgtttccctc ctgatctatc
    11521 cttcaatttc tatgaaatga gcgtagtact cacccgttga gccttttatt tcgcagcgca
    11581 acgcgccttc gtggtaaacc gaccctttac attcgtattt cttacccaga tacccgtata
    11641 tcaccgtcgg aacgttcgcg ggcacctctg tttccagtag ccacgagcgg ttggtttttt
    11701 cccatccaat tatccgcgga aaccgactcg gcccgtgttc ggaaaattcc gggacgatgg
    11761 ccatccattc gaaaggcgtt tgataatcga tctctatggt gacctcatcc gaacagagga
    11821 ttttgggtag gggtattttc acgtaatgga taccgggggg cgagtccgga acactgtagc
    11881 cggaagggta tgagaacctc ttttcagagg agacgcgtac gccttcgggt ttcggtctct
    11941 tctggcccac cacaacccgt atgttttcag cttgtttcgg aatatacggg gcaaagtcta
    12001 tctccaggtc ccggttcgaa cgcactcgat accgttgatg aacggttacc gcatccccta
    12061 tttcctggga tcgttcgtag tctagagaga tttcggcatc cccgcacttc aattgagaaa
    12121 cggtcaggcg agaaaggttc gggggaatct gtggagatag cgtaacggcc ttttttaacg
    12181 cgtccggaga aaaaccgaca accccttcga tccaaggctg caccgccata ctctccgacc
    12241 acgcttggtg caaacatacc ccggatgatt ggtagagctc gccgtgaagg acttctcgga
    12301 tatacccttt cgcgtaataa tccgaatcct tcaaactcgc gcagacatga gtaaaaccgc
    12361 ttagagccgc gccggcagcg aattctgaca gagacgccca acccgtaaac aaaggccaaa
    12421 cggagccctc gtgatatcct ccgggattgt agacgccgct gcttttcccg ataatcctga
    12481 ccccccaatc ggtagagtaa tcgtatctgg aaaagtcgcg ggcgtgcgcc tgcgcggcag
    12541 ctccggtcag actccccaga tagatgccaa ccgcaggtaa aacagtgaag tggtcgatac
    12601 gctctccatt gacatctatg ccgaggccgt aacgctgcct ttccggattg agaaacctcg
    12661 gaagcgcatg agctacctta aggtgtaaag aagagacctt gtcgaaggtc actgagtcct
    12721 cctctataaa aggcaacaaa tcgaccagtt cctttagaac gatgtgccaa ataacgttga
    12781 ggtataagct cgcgtgagaa aggtagagct tcccaccttc tatccaaccg tgcccggcaa
    12841 tggtgttttc gatgcacccg tcaccgtcgg tgtccgtcga cgcgcaatag tctaaggcca
    12901 tcaagagatt ccgcttatac cgtttgaacc attcgatatt cccggtatgc ttcaagtacc
    12961 gtatcgccgt taacaggaac aacggcgtcg cgtctgccgc atcataatga accaagccgc
    13021 tcgtcgacaa ttcgtgatag attttccccg tcaattcttg gtaccgtatc agcagctcga
    13081 gattttctcg caccgtttcg gcgtctcccc aatctagcat cgccagggaa acccattcgc
    13141 tgtcacgccc gaagtaccac gcgtatccgg gccttgcgcg gttccaaccg gaacggctat
    13201 tggcgtatcc ggcgacgagg ccggtaccca gaagcggcgt gctgactcgg aatttcagcg
    13261 tagcgatcat cgcgcgctct atgcgccggt cccattcgga atgatcggct cgtatccgga
    13321 cacagcgatc gaggtagtcc gcgtaataat gaacggatcg gtcaagctcc tcttgaaaag
    13381 agacgccttc tatgagctct tctccttcga tggcagcgca gagggttagg catagagaat
    13441 gttgcatcga aacagcgagc gcgccggtta atagattttc gtcggaagtg gcctcgatct
    13501 cggccgggcg gttcacggtc atacggcacg cgcaacggtg atctcctgtc caaaaggaga
    13561 gcgtgccggt ctgcgcgtct ttcgtatagc gccggtcccc actgtaatca tcgtccatcg
    13621 gccaggatat ccgcaggtcg gtttcgaagc gtaacgagat gcgatgaaca cccgaatctc
    13681 gcgcctcgaa ctgtatccca aggagcggtt tgtcgatgct ggcgaaaacc aacacagagg
    13741 cttcttcggt ctcaaacagt aggtaagccg gctctatcac ggtgttgatc aggagggacg
    13801 acaggggacg cccatcgacc gcaatctcca actttttcag aatccgatag ggatgcgccc
    13861 atacctcccg tatgtttccg tcatccacgg cgttcaccag aatacgcgcg ccggacgtgt
    13921 tcgtgtagtg gccggtccga agtttgggca ccgaaatgcg tagcggtgtc gcatccggaa
    13981 acctatgggc tttttctata accaattctt tatgagaggc cgttttttca aaaaaaccat
    14041 gggtgatctc gggccaacac gggtactctt ccgaagacgc gcgaagcatg tagtcttcga
    14101 tattcgcgcg aaatctttcc accagcgttt cgtatgggta atgccttcgc gtttccgggt
    14161 agaggaaacc cccgacacag aggatttttt cagacgggta ctcccatatg agggcgcgac
    14221 tcctgacgta gtcaatgaac cgcttttcta ccgcgatcac cttggcgttt tttccacaat
    14281 acaaggacac ttctccgtac ggcggatcca gaacgtcgag cagcacggag tagaaccctc
    14341 cgtgtaaacc gttaaatatc ggatggtgtt ggtagctttg gaatcctctt ttatcccaag
    14401 gccctcggat tttttctata ggctcggtct tcaccgcttc ggcgttatcg attccgagac
    14461 gggccaggat ttccagcccc ttaccgtata atacgacggg ccgctgcccc cttgcccgat
    14521 gaacggcctc tctcgtttct gccgaatgct cccctatcca taggatggaa gggtttcggg
    14581 atgtggtgtt gatcatctat tgttcctcct atcggtcgta accggcggtc atcttgattc
    14641 cctgtacgat ccatttctgg gcgaagaggt agaccaagat catcggaatg gaagacaacg
    14701 tggcggcggc taactgagcc ggaatattcg taatatattg ggaagagaac atcgccacgc
    14761 ccacttcgat cacccgcatc tccttgcttc tggtaacgat gagaggccag aaaaaggaac
    14821 gccacatact gaggaaggta aaggtgccga ccgtcgcgag aacgggtttg cagagcggga
    14881 ggatgagctt ccaaaagact tgccaccgat tcgccccgtc gattctaccg gcatcttcca
    14941 agctgctcgg cagactgagc atatactgcc gcatcaagaa gagcccccag atattgccca
    15001 acgacggaac gatgagcgcc ataaacgtat ccacccaccc caacgagtga aggataataa
    15061 accccggaat caacgtgacg actcccggga gcatcataaa ggcgatgaac agtttgaaca
    15121 taaaatcgcg accggggaac ttcatcctcg ccaaggcgta cccacccatc gccacgagta
    15181 ggagttgccc agcgagcacg gcgacggtcg cgaacagcgt gttgaagata taccgccccc
    15241 aaggctgcgc ttgccacatc tctatatagt tgctccagcg tagaatggtc ggtataaatt
    15301 ttggaggtat ttgatacatc tcgaactccg ccatcaacga aagggcaatc atcaggatgt
    15361 agggcaacaa cgtagacgcg gccagtagaa acagcaaggc atagatcact atttgggtga
    15421 tcacagacag acttctgtca aggtttcgtc gattcatcct ccacctcccc agtcctaaaa
    15481 atacgcctgc caactgtctt ttccgtacag tttgaactcg aagtaggtta agaccatcaa
    15541 gagtaagaag aggatccacg actgcgcagc cgcgtagccc atacgcatct cggtccaagc
    15601 cgccttccaa atatggtaaa aaacgacatc cgtggaccgc aaaggccccc cttgcgtcag
    15661 aacgtacaca ggggtgaaca cctgaaaaga gcctatgatg ctggtaacga ggacaaacag
    15721 cgtggttggc ttaagcaggg gcaaggtgat gtgccggaag gccttgtatt tcccggcgcc
    15781 atcgactttg gccgcgtcgt agaagtctat aggaatcccc tgcaaaccgg ccaaaaagat
    15841 taaaagctgg ctccctatcg cttgccagat gctgatgatc attatggaca acaacgcgaa
    15901 ttgcggcgaa tcgatccagg cgatctttcc gatgccgaac catccgagga tataattgag
    15961 tattccgaag tcccgattga agatccactt ccacacgatc gtcgtggcga ctccggctgt
    16021 gacgacaggc aggtaataaa gcactctcag cgcgacggcg atctttccag ctttgttgac
    16081 ggccatcgcg agcagtaggg aaaacagcat gccaatcggc acgtaaagag aataaaccgc
    16141 cgtgttcttt agggcgttcc agaaattgcc atcttgaaat agctcgatga aattgtcgaa
    16201 gccgacgaag ggttttgcgg gatcgacgat gctccaacgc cggaacgcca aaaacaatga
    16261 aaagcccaaa ggccccgccg cccaccatat taagtgcacg agggcgggag ccagaaacag
    16321 atatccaaac aacgtgttcc ttatgagtag tcgattatga agatggcgcg tcaagaaccc
    16381 aaagatgagg gcaacggcca agaacgccaa cagaagaatg actcccaaga actgcaccga
    16441 cagtatctcg ttgctgacga aggtatcttc gtggaggact tgccaatcta actcgcgcga
    16501 tcgaaaccgg acgtaagtct gcccgatacg gtcgatgctt aaggtgccga tctctatcgc
    16561 gccggagcta tcggcgggcg cgagcggaga gatcaatcgt tcttgccggc tcgagagcgt
    16621 tgtcacagtc gaaaagcggg aggtttgatc tacggggcag attcggtagt catcgcgggt
    16681 cgctcttttc atttgctcta ttatcgattt cgaactcagg gcgatgtggt caaaacggta
    16741 ttcgatatct tgttgtaaaa ggctcccgtt cgccaacacc gatgatgact catactcacg
    16801 tagaagcgag ggcgcgagca ggaaggcgcc atacaagaag aaaaggtaaa tgggaaaaaa
    16861 ggccgcgatc gcatagaaga gagaggcgtt cctcaggcgc ggagaacgat cagcggttct
    16921 ttcgcgataa tgcaatcgaa tcaaaaagaa aggcgccaca aagcaggata ggagcgatcc
    16981 taaccacacg aaccaatcta atcgatcgga accttttttc caaaagcttt ctggggtgac
    17041 ctgaacgaga aatcccttca catcccaatg gtccgcgtcg tagagagcgg tggaggtaac
    17101 gagcgatcgg ccgcttctga gagaaaaaag ctttgcagaa gggcgctggc tatccacagc
    17161 agttgtaaac atccgagaat actcgtccct aaaaccctcc tcgaaaaata ccgcggagcg
    17221 ggaagaaaac ggaaatgggg cgaaccgttc ttcatccccg tagaagaacc cgtactcgcg
    17281 gcccaacgcg gcgttaagtc tccgaacgaa atccgccaaa acatcttgac cgacgaggac
    17341 gatcgacccg tcttccagta gaatagcggt ttgtaggtgt agggtcccgg tatcgtaact
    17401 gatttcggac aacgcatagc cctctgaata gaagagagtg tcataaaaaa ccgtcagcac
    17461 gaaatcctct gggactttcg cccctttcga ggcgacgagc tcgccgtctg ggccaaaaaa
    17521 acacgcgtat aaaaaaccgt ctcgaaagag gagtgccgca cgatcgacca ccgctcgaac
    17581 cgattcgggc gaatcaatga cgctattttc aacgagttcg tttaggcggg cattggcttc
    17641 ctcaacggca agagccaggc tgttttcggc gtatagaagg gtgtttttct cattttgagt
    17701 gacggtggac atccaagccg aggacgcgga cgctctccac gcgaagaagc cactaacgat
    17761 cgtgataaca gccaccaaac agacgatcca cattttcacc ggagttttca tgaccaacct
    17821 ccgtcttgtc tcagcagaga taggatagaa tccttcgtga ccgaagaacg cctaaataga
    17881 cgccacggac ggttttcgtc cgtggcgcaa aaaaggattc ttcttacccg taaacgcgtg
    17941 tggtactagc tttctccttt ggctatggct ttatccattt ccgaggccat ccaatcgagc
    18001 acacttctca aggattcgcc gcccacgttg atcttatcga aggcttctgc ccataggtcc
    18061 tcgaatttcg gtcgatagaa ctttgtttgt gacccgacgg gcacccgaga gtaatcgagc
    18121 attttgataa aggccgcatc caacggatcg ttttcaccct gtaattgcgc gaccgcctga
    18181 tttgcggaaa tggcgaggct tccttccaaa cagcgtttcc tttgcgcata atcgctggaa
    18241 aggaaggcgg ctaacatcac ggctccccgc ttgtcctgaa cgttcgaagg cacggaccag
    18301 ccggacaaat aagaaacggt gtccttttta ttgatgtcgg cgaatggtag ggaggcgata
    18361 cctacctgta aatccagtcc cttcgcgatt tgtcctttga tagaaacgtt gaaccagcgg
    18421 ccggttaaaa tcatacccac cttgccggta tagaatagtg cggaatttcc gcctaaagcg
    18481 gaagcttgct ggggggtcgg cgcatatcct tctttcaata aatcgaggta ggtttggtag
    18541 gttttcacgt tgatatcact gttcagatac ccgctcgtat gcgagaaatc cggactgaac
    18601 acctctcctc cacccgccca cagcaagggc accgcgattc cgacccagga tgggaacgcg
    18661 aatccccaag tatcgttctt tccgtcgccg tccaggtctt tcgtcaacgc tttggcggtt
    18721 tcgatgaact ggttaatcgt ccaaccgtcc tcgtcgggat aaggaacctt catttcgtca
    18781 aacatcttct tgttgaagaa tacccccatc gggctggtgt ctttcgggat agaatagatc
    18841 ttgcgatcga tacgggcgat gtcgaccaag acggggaagt actcgtacac gttgctaccg
    18901 ccataccctt ctctcgcgag tctggagagg aaaggcgcca ggtccgtcaa gaagtctccc
    18961 tcgaaaaatt tcgggatcat ctccgcgtcc agcaaaatca cgtctggggg cgtgccggcc
    19021 ccgatgtccg taaggatttt ctgataataa ttgtccgaag ctacggcttc atacaccact
    19081 tcatagtcag ggttcaagcg cataaattca gcgataatgc tttcatccaa tgcggcctct
    19141 tccgctccgg cccaggtggt cacgcgtaat ttcgtcttgc cgaaagcggc cgtagaaagc
    19201 gcgagaatca ggagcacagc gatgatcaca cgaatccagt tgcccttcaa taacgtgttt
    19261 ttcatactct ttgaccctcc ttctgtaagt gtaagctact tttcctaacg atgagctcga
    19321 caggtaagat ttttcgctgc gttttctttc gttttccttg gagcctgttt aagagcatac
    19381 ggaaagcttc gaaacccatc cggtgttttt gtatgctcac ggttgtcaac gggggataga
    19441 cccacccgga atactgtata tcgtcgtatc cgacgatggc gatatcctcc ggaattcggt
    19501 aatggttatc ttttagagcc tgcatcgcgc cgaaggccac gatatcgtta tagcagaaga
    19561 taccgtcgac tggcatccct ttgcaaacgg tatggagtat cgcccgataa ccgtcttccg
    19621 gcaggatatc tgaaatgtga acgtaggaag ggtcataagg gatcccggca gcttccagcg
    19681 cctgtcgata gccttcctgg cgcatatgcg acggagattt gtaactataa ccgtttaaga
    19741 gcagtatttt tctacatccc tgatcgatga gatgctgagt ggccagtttt cctccgttga
    19801 tttcatcgct gtagatttcg tctatctcca tttgatcgaa gtgccttccg acgatcacgg
    19861 tcggatagtt cctgtgaatc aggtcctcta tgtgtttcct tgattcctgt accgggccga
    19921 taagtaatcc gtccactctt ctctccatca gtagggagac cgcacggatc tcatttttcg
    19981 tggcgcgctc cgtgttgacc aatatgatct gataatcaaa cttcctggag gcccattcta
    20041 tgcctttcag aacttccgaa taaaatggat tagaagaatc tgccatgatc acgccgataa
    20101 tccgtgtttc cacagaacgc agcagggatg ccgatcgatt tttcacaaac ccgatctcct
    20161 gagcgatctg gagaattcgg gctttcgtct cttcgcgtat gtccggtttg ttgttaagcg
    20221 cgcgcgagac ggtgttgata gagaacccgc tttttttagc cacatccgcc atcgtaataa
    20281 acggcttccg catgcatcac ccgcttcgac cctttgttgc ctcgtgaaga taaggcttcc
    20341 gggtatccta aacgtttccg gtatcattac cgtaaacgtt accggtataa tagcacaaaa
    20401 aatagacgtt cacaacacgc gttctttcta tgctatgatg aataaagtcc gtatatcctg
    20461 aattattttg gtttatctgt aagtctctac ggttttcggt ctttttcccg tatttatggc
    20521 acgaggaaat ataaaaaaag gcgcttagtt ttggcgcgcg ctgccgctat ttagaccatt
    20581 ttcctcggaa tcgcgggggt tccgaaggcg gtcaccccgg atgggacgtt acgaagcacc
    20641 aagctgttcg cgcccaccac cgaccatgcc ccgatcgtga tgttctgtag gattttcgcg
    20701 cccacaccca cataacaccc ttcctctaaa accacgccgc ccgacaggtt gaccgaaggc
    20761 atgagagagc agtaatcgcg tatgattgca tcgtgtccca gctgcgtgcc gacgttgacg
    20821 aagactccgc gcccgacgac cgcgttgagc gaaacgaagg tgaagggcgc caacaccacc
    20881 ccttcctgta gttttgccgt atgcgccaag aacgccgtcg gatggacgat ggtcggaaac
    20941 gagatattcg ggtggtccga aaggctctcc atcagccgcc ctttgatttt cgtatccgct
    21001 atccccatca cgagcgctgt gggagcggtc ttgcgcttaa gcgtttcgag tccgccgagg
    21061 acggggtacc cgtctacgat actccccgcc tctatgccat cgtcgtagaa cccctcgatg
    21121 acgaactttc cgtggatttc attgtattgg tgggtatagt agaggatctc gcgcccaaga
    21181 cccccggcgc cgtatacggc tagtggaagc ataggatcac ctctcttggt gaacggattt
    21241 tcgccctcta ttatatcagt tttctcttcc gtcgagcgat tctcgtgtgg gtcaagttat
    21301 ggggtgacat aacaaagagg ttcatcgtgg ggtaaccagc gggcagactt cggaagccca
    21361 cgaagtctcg cgatacgctc tgctgtcttt cttttccaag gcatgccaac ttaaaagttg
    21421 gcgttcccag aagacatgcc gaactgaagt tcggcgttcc cagggcattc tcagagagat
    21481 aggccgactg gaagtcggcc ccacgagttt tcttttgtct gtgttctctg tgccatccgt
    21541 gtgacccggt tttgatttta agacctgcca acttaaaagt aagcgttccc agaagatatg
    21601 ccgaactgaa gttcggcgtt cccagggcat tctcagagag ataggccgac tggaagtcga
    21661 ccccacgagt tttcttttgt ctgtgttctc tgtgccatcc gtgtgacccc gttttatttt
    21721 taagacatgc caacttaaaa gttggcgttc ccaggcgttc ccaagcgttc ccaggccttc
    21781 ccgagcgtat cgcgccgtcg cgtaaagaag gggcgctttc gccgcgagga ccatatggac
    21841 taaaaacgcg agatcgattc tagccttgtt tcttgatagc gcctcataac ttgacccaca
    21901 ccgtcgtttc cagaaacaca atatttgtga tagaatcata tagtaatcgg ccgctttgac
    21961 cgccaaccgg atggtgacgg caggatccgg cgggtcgatc gacgtttctt tgacgtcgtc
    22021 gcgcgccaaa gcggcgcgtg ataggagaga gcgtcacaat gactctgatc agccgagggg
    22081 gaaaatgcac ttacaccaca tcggatattt ggtcccttcg atcgaaggcg cgatagaatc
    22141 tttcgagtct ttggggtttc aaaagcactc agagatcgtg aaggatgaag aaaggcaagt
    22201 cttcatctcc tttctcactg atgcgaacca cacgctggtg gagctgatac agccggtaga
    22261 cgaacggtct ccggtttttc ctctgatggt acgacaaggg cctggtacgt accatctgtg
    22321 tttttctatc ccgcaatcgc aaaaggcttc cgccctttcg cggttgaagg ccctgcggtt
    22381 cgttccggcg ggaaagatag ctccggcgcc cgcttgcgag aaccaagagg tcggcttctt
    22441 ctattcgaac aaaatcggtt tgatagagct gctattcata tctgatacgt aatagggggg
    22501 aaaagatggg accttttgaa gaatttgcca taaaaatcgc ggagatctta gaagtagacg
    22561 aaacgcagtt aacgcctgaa acggaatttc gagcagccac gccatattgg tcgtctttga
    22621 tggggttcgg aattctcgtg atgctggaag aagactacgg acaggtgatg tccgtcgacg
    22681 actttctgaa agccaagacc gttggagagt tgtaccagcg gacagaaccc aaagggtgat
    22741 ccgggatgga agagagaatc ctgcaatggc tctctacgat tcttcgggtg acggtgactc
    22801 gagacactac tcgccaaaac accgaacaat gggactccct acgccatctt caaatcatcg
    22861 ccggattgga agaggagttt gggatcgacg tcccgctcga aaacgtggcc gagatccttt
    22921 cggtacccga tatcttgcgc tatgtcgaag ggacgaattg acgtggcgac gacgataacg
    22981 ggcttcaagg tggagggact ttgcgcctgt gtgccgaaag aagagatcga caaccgcgaa
    23041 tacggaaaag cgctctttgg agaagacttg gagggtatcg tccagaccat cggagtctca
    23101 aaacgacggg tatgcccgga aggggaaacg accagcctgg acttgtgcgt gtgcgcagcc
    23161 aaaagactgt tcgagcgcgg cgactacgat cccggctcgt tcggcgggat cgtttgtgtc
    23221 acccaaaccc cggacttgac gcttcccaac aacgccacat acgcgcaaca tctgctgggt
    23281 ctgccacagt cgacggcagc tttcgacatc aatttggcct gttccggtta cccgtatggc
    23341 ctctacgtcg cgggtatgat ggccagtacg atgcgaaaac gcgttctgct gctggatggg
    23401 gatacccatt ctcatttcgt ctctccgcac gacaaaacga ccgctttgct cttcggagac
    23461 gcgggcagcg ccacggtgtt ggccccacgg gacgttgagg ggtccgaaac atccttccta
    23521 ttccatacct acggagaaag cagacaggcg ctccatatcc cggatggggg ttaccgaaac
    23581 cgagtgaacc ctcacagcgg cgagtaccgg gaatatgaaa atggaaacag gcgccgccct
    23641 atcgatatgt atatggacgg gatggccgtc ttcgacttcg tcgcgaaacg cgttcccaaa
    23701 gccttacaac aactcttgga cgaaatgggg aagacagtgc gggacctgga ttggctggtt
    23761 ctgcaccaag ccaacctctt tatgatcaaa caggtggcga aaaagatcaa aatggatctg
    23821 accaaattgc ccgtgtgcat acataaatat ggtaattcga gctcgactac ggttcctttg
    23881 accatcgcct cggaattggc cgcttcagta cgcgaaaagg cgaacaccat tctgatgagc
    23941 ggttttggcg ccggtttatc gatagggact gcgttattgt ccatcggccc ctgttgctgt
    24001 cccggggtgg tggaatatga accctaacca acccttgaac gttctgatcg tcggaggatc
    24061 cgacggtatc ggtcttgaaa ccggcgcgct cctctgtgag agagggcact gtgtgacgtt
    24121 ggtcgctagg gacttcgaac acaaaccgga aagggcgctc cggatcattg cgcgcctgaa
    24181 ccaaacctac ggagacgcgc agtgccgctt gatccgccaa gacgtttccc gcctggagga
    24241 actggagtcc ttttctaaag gcgtaaagga gcgcgtcggc ccgcttgacg gtttcgtcta
    24301 ttgcgcaggc gctcagaaga ccctgccgtt ggcgatgacg aaaccggccg cggtgcagga
    24361 actgttcaca ctcaacacct tcgccgcgat ggaattggtg cggtgcttct ccaaagtcgg
    24421 attcgttcgt gcaggcgcat ctttcgtcct gctctcctca ttggcccctc acgaatcttc
    24481 gccgggaaaa tccgcatacg ccgccagcaa ggccgcgctc gagggcttcg tcgcatcggc
    24541 ctctactgaa ctaatgcaaa agaatatccg tttgaacgcc atcgcgcccg gaatcgtcaa
    24601 gacctcgatg agcgaaggct tcttgagcaa aatgaccgtc gatcaggttc gagcgctcga
    24661 agacacctat cctctgggct tcggtgaacc ggaagacgtc gcgtggatga tcgagttctt
    24721 gttaagcccg cgcagccgat gggtgagtgg aaagaccttt ttaatagacg ggggacattt
    24781 accggggggg aagtagagag aatgaaaaag atagccttgc tttcgaacgt gacggtggat
    24841 tcggtgagaa accgcttgga ggataaggga atcccgggtg tttactgcgc ggaagggtac
    24901 ggcagttggg ttgaacatct acttccaggc gggaagctct ttgagcagcc gcttgatggc
    24961 gtgatcgtgc tcttggatgg ttcagcgttg ctcgagcaac acggggagga gaacctaccc
    25021 ggttttttgg aaaacgccct cgccttgatc cggaactcga aagaaaagct cgggaaaacg
    25081 gtatggatcc tctctacctt ggatattcgc caagaaaaga tactcccgct caccgcccgg
    25141 cgattggata gagaggcctc cgcgctctgg aaccaaggcg ttcgggaatt gggcggcatc
    25201 gttttcgatt tggaagaact cgtcaaagaa gtcggacgcg aacggtttta cagccgaaag
    25261 ctgtggtacc tcggcagcat cccgttttct tccgctggag aagaaaggat agccacggga
    25321 ttggcccgac tgtggcgcgc gtatcaagga aaacgaaaaa aagtgttagc gatagacctg
    25381 gacaacaccc tttggggagg ggtcgtcggc gaggaaggga tcgacgggat cacgctttcg
    25441 acagagaaag aaggaaaggc tttccacgac tttcagaaac gaatcctcga tttgaagcgg
    25501 atgggggtga tgttaaccct cgtgtcgaag aacaatccgg aggatgcatt agaggtcttt
    25561 cggaaacatc ccgcgatggt cctacaggtg gaagacttcg tgtcaatgaa aatcaattgg
    25621 gacccaaagc ctcaaaatat cgccactctt gcgaaagagc tgaacgtggg cttggactcc
    25681 tttattatga tcgacgactc ccccttcgaa cgtcaagcgg tgaaagaaat ccttccggag
    25741 gtggtcgcgc cggacttccc caaagacacc tcgaaactct ccgactggat tagggaatta
    25801 gcgtcagagt atttcttgtt tttggaaacc accgaagaag acgcccaacg aacccagatg
    25861 atgcgcgcgg atatcagccg caaacaggtg cagcagcaat tccaggatct cgaccactac
    25921 ctttcttctt tgaatatgac catagacata cacctcgccc gcgacgaaga cgttccgcga
    25981 atcgcccagc tcacccaaaa gaccaaccag ttcaacgtga caacccgccg atacaccgag
    26041 gccgatatcc tgcgtatgaa agaagacccc gcatatcgct tgtggatagg cagtgtggaa
    26101 gatcggtttg gggattacgg aaaggtggtc ttgttgatcg cgagagtcga agataacgaa
    26161 gccgactacg ataccttcct gatgagttgc cgcgtgatgg aaaggcacgt ggaagacgct
    26221 gctatctcgt ggatagaatc gcaactgggt atggaaggcg tcgaaagggt tcgcgcgcac
    26281 tacttgccca cgccgaagaa caaaccggtc gaagggttgt ggcagcggtt gggctattcg
    26341 actttggatg gcgccgacaa cttcgagagg gacttacgca cgccgcttcc cgcgtctgcg
    26401 atccgaaagg cgcacgtcaa gataaaaggt cagaagtctg ccgtcgacct ttcggaggaa
    26461 aacgcataag cccgctctcg aaaagaccgt gaagtcgtct cgtggcgccc agcgcggaat
    26521 cgaactttcc caaatggtgc gggtcaagtt atggggcgac ataacttaga gggtcagcgc
    26581 gcggcaagtg gtttttacta ccccgcgaac cacccttcgc cccttctacg ccgcgatgac
    26641 cttgtggagt tatcgcgggg taaccaacgg aaccccgcga tactaatgaa atctaacgat
    26701 gcgctctctc ctgtctttcg cttttaagac atgccaactt ataagttggc gttcctaggc
    26761 tagaggcagg ccgactggaa gccggccccg cgagttatct tcgatcttt
//
LOCUS       DAOPXN010000005        26345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_1835_length_26345_cov_5.117763, whole genome shotgun sequence.
ACCESSION   DAOPXN010000005 DAOPXN010000000
VERSION     DAOPXN010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 26345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26345
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_26345_cov_5.117763"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1383)
                     /locus_tag="PKM49_00685"
     CDS             complement(112..1383)
                     /locus_tag="PKM49_00685"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNY81361.1"
                     /translation="MIREAYVETVGEICARLRGGAIDSTRSREITRGSVRLYENGKIG
                     IAGALGEFDEEGLTQEAKKRLDMGVVYTASPTADKHEEREYIETDMDAYDFLWEVEEM
                     LGEQNRRFPDCSFVHDIRYISESRHLRNTVGLDLYHKARYFEFYLTLKRKDSENVKDG
                     TLSFRLPSFDKRAALDALRMLCDGLGQKPCAPEGRIPVLFFFNDPLYNKTLWETLNGI
                     SYGSGSSLYAGRMGEKVFADGLTLYQSRNRKDQFFNAFFDMEGTTNPEDRCLLIESGV
                     FRMPFTDKMTATRFFLPLTGAAQAEYDSVPSMDYPHLAYQPSEHSLSELLSQSQRENS
                     GRALIALQPTGGDFSSDGHFSLRLQTGLWYDETGCDAPVHNISLTTEIHKMLGDDWIG
                     VPRHPLFHIPGLYGVCFWMTVQGPSPSDGRL"
     gene            complement(1390..2733)
                     /locus_tag="PKM49_00690"
     CDS             complement(1390..2733)
                     /locus_tag="PKM49_00690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNY81362.1"
                     /translation="MFEFPDSLYCDVRIEEVFQTRIAFGFGDVQEMLERRYEAAFVRV
                     FDGKRWYYSATSSVDAVQEHIDRLASLTTPSQAVSEHPVVQKLEAHRETLRQFSGPQS
                     VSSKTIKEKYDLLSHYFPIIDSYDHIRLWTARYVDELRNKRFISSKGADCSFDYQKIG
                     LLFSYEMSAKGNQIKDSFSYGAHVYRDMEAQDRKFANTLNRSADFLKRAKSVESGRYP
                     VILSPQAAGVFAHESFGHLSESDTYQGDQTLKDRWVIGKQVAHKNLSIVDDGGVLGMG
                     YTPFDDEGTKARKTYLIRDGVMAGRLHNASTAVDFGENLTGNARAISFEYEPIVRMTN
                     TYIEPGKTPLCSLIGEVDNGFLIDTIKSGFGSTNFTVSPIIAYRIRRGTIAEPVKISL
                     LTGNSFGTLFKIDGISDRASLGENPFVTCSKRDQLRLPIDFGGPYVRISEIDVVG"
     gene            complement(2864..4087)
                     /locus_tag="PKM49_00695"
     CDS             complement(2864..4087)
                     /locus_tag="PKM49_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNY81363.1"
                     /translation="MRIGDFAQKYDISIDTVRYYIENGLLLPEKIHNQYAFDRSCEED
                     IERILVFKKMRFTITEIKMLFSLHRLTQLVDEKDRDYFESYFIKKREGLLLEKTALDE
                     ALSLINEEIAQLQTQKLTVYRPLGIPISFFSAFACPRCAEPLEVGKASVEGNMIYQGE
                     LVCSCGYQASIEDGIVITPSSGAEGGPSYLQEENPVRAYLDSTDPLFISFTKKGFDWV
                     MRRVAFENRPLSVLEIGSGFCFFLMGFIDRLPDKVTYIMAENHLSLVRYAKKHFETHY
                     PNKRLIFICADFNELPLRKESVYCVFDMFGSSAHNFDNDFYPVKNVLPLLKNEGYWLG
                     SYLYFEPEEAELLPAYRKYRRMFDLERIQLAFSELEKIESSRLAEIQVTPDLLDRFRK
                     DSIVSLWVYKGQKMG"
     gene            complement(4337..5290)
                     /locus_tag="PKM49_00700"
     CDS             complement(4337..5290)
                     /locus_tag="PKM49_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY81364.1"
                     /translation="MALLRRKWFFDPAICSLSIPDGGYQSSMTQKRYRFFLFVLLLFL
                     CLSPLFASEPVRIFWVTDNHLKYGDSALEARLEWFIDEVNDKRPDIVIHTGDAIDGKR
                     GRALALKEIDRFAHYWDRIPDSVLSLFVPGNRDIGSAYETTELDWLFLFPSTQETAGS
                     FFNRIVDYQKGDVRLRFILLCDFSQTLDRAELDCWLADALSAPEVTALFVLAHSPSLY
                     SLVVRLLETSPTRDQPVYFLHGHWHGGLGMTLQEEDSELKVSRFLLGGLQDGYYGAVQ
                     SLFQKVLPFLIQPPFYERLLIDETGELTHQTIWQSADSAAF"
     gene            complement(5943..11372)
                     /locus_tag="PKM49_00705"
     CDS             complement(5943..11372)
                     /locus_tag="PKM49_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81365.1"
                     /translation="MKKTAMVAFIILFFLLLFSSCVLQDLFGGTSRSVRIKVTMTPET
                     PSYIGQVLKVLVSTTPRKELPYIRLTTKNFVTGEERSYMQENVGSHEFSIKLHSSQFG
                     FFIETPGRTRQNGNAIWWPSKETYIETEDFTPPNVSVKVSRVNENGNDYTFEVIADES
                     ESAVIRRWVTVNGTEYELEGQRQSTRLPLSIGTYIIRGFAQNSYGYVGSSSNKYVEVT
                     PPRSNEPARIGWNHPKQLTPFTDENVTLSAQITDNISYIKRIELRSLSGIKKTYQFDP
                     MVPTYSLRYNLKATRSEKVTLTVTNGNNIVTTDEVDLSIQSHRAPKVTLEGPAGSFPI
                     DESKSYLLKIDTYDGALIDNARFLVNNTTASAFPGIGESSYKKTLTYTVLNGKKYLKA
                     IATDSWGATGQSDEIEVIGYKIDKTPPRIQLYMPSVAYKNVQTDLSFLVQDSDSDLAG
                     IPGLSVLEDNLTLNPLTYDNAFYFAKWTPEGTGTRTIQVTATDTAGNQATATASIQVK
                     DPTGIVRPTVGALSAYPNVLMLGSSVSLVLSVTPPSQNPGGIVPVVEIAVTAPNGGTP
                     YKIGSISQAGYIYTGTYRPAVAGVHTAIALIQWGDDQYTKSCTFRVDAPKPTMVFTVE
                     PEETYIGNPVTLKLSPDTANPNASLTVLEMSVEEEALSWVRTIGAGGSVIYVATKSTL
                     DMSPGIKKARAKIRDSFGEEITTTATFTLRSPNLRITGYKYVTDSGKPPNAYDPTRIE
                     VYMGADLPAAIPINGEVAIARRGSSDERGVAETYPLQRTPSDAYTFVTKEKWIPKEAG
                     LYDISFRLSGVIGSQATETKLATEVRVAAAQLNVGISATPVEIDKIRTGEAFSLVFTV
                     TGATVVDPIKSFTYGISSGSAIHQTETAPTQMGSFTYTHTPPKFSVAGSYTITATAVT
                     ISGNTKVLTLTIEVKAPDISISIEASPAIDRITVGRPITLLFSVDGIPNSDKIDSFSY
                     AIWKGSEIYKDAVYLEHQRATRYTDPIEPFVTEGVYTIIATATTAGLSVGVNSRTFTV
                     LSAEIRKKTFALEGSPSQLFIGAPLYFLLELENPNNVSNLSVRMYITDATGVSIPGVV
                     PIGASPTNNNNTTFRNNKPFYISQPGTFTVYASVTLNQVPQIDLPVMRSDSDYAIPPS
                     TVGVSIDTSKQYLAGYEGSLRVTVTIPQGMNIQSPRVLLENSNLGEIDPTPYSSDVSG
                     SSAKYYNFKFRPTIPSSPVVVISIYDTSDLSGTNPLTTKRATLTVVPFNPQVRITSVG
                     NVAFIQAKEPRVRLEIDQIPEFFTGSYEYLCSLSTYDLTVTANEPSILIDLPVQALFN
                     LKGTQAATVTAEVKATGNGNFIAKGQETFDVVLPSFSNLRFPSDVTNGTYFIYEQEQL
                     RVVFNNDQKVSDNSLIQVRLEIEGNPNPAVYIAAPTLSSTTIPASALFDQVVFSKVGS
                     IWATPTIYCLNTNPSSVLATSSGEQLNVYPSTPRVEIIQPSTIDPLFVGQVIKPIVRL
                     IRVPPVVTHVTIALWEEGSSEPPLTRDLILSENDDWIVYSGDPPIVFTEPASFTLVAT
                     ATSSANPNVGYVTERKFNVVQGDLQTRDLSIADGGSLLFRDEKVRFKFEYAFNNPYRV
                     PSTLATLTNVSNAEIAPPNIVRESPPSTSMNGDYIVFTEYWVIASNTAASGNYRLELV
                     AFSGDLKRSRTTEFDILQALTINDSLISLAPELVNEGDRITGKISITLPSDFITAVGT
                     DNIQPYLYGSVVFEGCDSQPTNHRLYTDPDGNPVWIIEAEGIARRHSDTPGATRQFYA
                     RYSVTLDSVNVLSVRKPFSIKNNTP"
     gene            complement(11489..13330)
                     /locus_tag="PKM49_00710"
     CDS             complement(11489..13330)
                     /locus_tag="PKM49_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81366.1"
                     /translation="MYEAKRKGWRPVFWSSVFLILVLVMTSCVLLTGRGLSGQSTGNA
                     EKYGIFKFLDPETGRPLSGPFTAPASFVVDFAYKGRLPVIKLELSAFRTEDNAYWLGT
                     EAANTRTATCLFYSEDNAGHFSFYATVTDFYKNVRTYERSLKIADPTPPVLERIEFYT
                     TDEATLIPFEKNLAYLWMQLWDAQSPIFNDDTVGALREKTRFYLNGKDITSTFWYAPH
                     EIFTTGAKNRLICIANVALEGMKKGENELRMVMGRYQDDEQDFVFATSTISSAYPDTT
                     PPVFLDYDFQPQEPGDFFFDFKVMDEFYLLLSVKDLPDKPNYEASGLKELSWTVEGEG
                     KKLSGVYPCDSLIEMNATIPIRLRQNAFPDGVYSLYVVASDNMGNKAKLGPVYFQLGV
                     TQYESLKIIDEKIGGNALLSEYRVGDTIRFTIDTDYTFDSVPVWNIYPSNAQFPQNAL
                     ESMYTNARIGNHEITVTAIHKGIQCFGRKAITVLATPVWEDDIPPVITISELVVDDPS
                     LPFVVTVTDDTSLGTTWEEIIRPTYYAIKEPVATASGTTATPYPLQVSFLPLGNGTRF
                     EQKFQFFLSDPLYGGNPTLQIGEKIKVYLEAQDENGNIRASSILFWK"
     gene            complement(14225..14824)
                     /locus_tag="PKM49_00715"
     CDS             complement(14225..14824)
                     /locus_tag="PKM49_00715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HNY81367.1"
                     /translation="MIHSIKGKIVERIEGGLIILENHDIGFEIWADKELYDTGDVEVK
                     AFVIFQPSESEWRMYGFLKREKRAFFQLLTTVNGLGAKTAMKILYDASVREIARSILE
                     NDLTYLSTLPNVGKKTAERIVLELKSKVKQVLPLLEETQAEGDALDLSLFQQATEALE
                     ALGYSPVQSRRVLRELFSEKKQWGMEELIKAALKKFFEK"
     gene            complement(14932..17130)
                     /gene="pulA"
                     /locus_tag="PKM49_00720"
     CDS             complement(14932..17130)
                     /gene="pulA"
                     /locus_tag="PKM49_00720"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNY81368.1"
                     /translation="MIGLALLVQKHELWVSVTNTYRDLSPDHFELSVDHVGAKIESVE
                     RIDPSRWAREFGWKEGWELWSIRFSSSRVDTLTANLRISFLWNGHTVSQKTPALFRTL
                     LSDYHEAGTPFSSWENCFPKCTETESQFRLWAPFAKKVSLLFYDRPEALKPSWLLSMK
                     RDTEDELQGVWRLRVSGNLHGTIYKYLVESGGATHEILDPFSISVTTNSRHSVLLDRR
                     YLDPPGWETDSHVFLPKYNDAVIYESHVRDFTVDPRTDLEAKGSYVAWTFGGSKTPEG
                     HTAGLDHLVELGITHVHLLPVQDFASVDDTHRADYNWGYDPLCYTVPEGSYSLDPRDP
                     ARRILEMKQLVQALHSRGIGVIMDVVYNHVWEPNGFSLTPLFSEALFRLNIDGSFSNG
                     SGCGNEFDTKHPAFQHYFSETLRYWVETYHVDGFRFDLMALIDRGTMQSAISGLRLKY
                     PHLLLYGEPWTGGSTPLHPLERSTGDFEKEAPIAVFNDRFRDAIKGYPDDESTGFVTG
                     RFDRLDEVLTGMLSMESWEEKSYSDPWKTVMYDSCHDNLTLFDKLRKSRPDLSIPELV
                     TRVKLCHFLVAMSHGIPFFHSGEEFCRTKYGQSNSYQSGDLYNAIDWGRKDTFAEVFH
                     YLRGLLVLRKTYELLRPKSGKAIARNVYSIERWRDGFFYVLKGKKTEGWLLIGVHLGN
                     SERDQKIPDGEWEIYANSLTAGTNLLGVAEGHLYFPPYSWFFAVKKPVTE"
     gene            complement(17127..19607)
                     /locus_tag="PKM49_00725"
     CDS             complement(17127..19607)
                     /locus_tag="PKM49_00725"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNY81369.1"
                     /translation="MFGLLSKFVYRFWKEILLVGVLVSILMTYLSLQMSIKTDLLVLL
                     PHNDPYSQRYKELFTGETVSETLMIVVDIDGRTFEAMEAAAQIKNELEKLTGIVKSFR
                     KLDTISLMGPAGIMLTHANLFEKLSSTLNFLNFLIANPAAVDFGIIRQMGISMNKVMD
                     LLREMERIESLDKYALISDDSQILTMTVVLTRPALDLAFTQRAVSLLKEKVGAILKPY
                     GFSFGFSGSHQGALDSQRIIFGDFSVTTLLTLGLITVLFLVSYGNISITLGIFLTLVT
                     GSVFSLGAFYLIFQSLEFTSSFVLALLLGLGIDFGIHLSNRSMEYLLENYPGFNALSR
                     KKKQEAMREAVQYAIQSAGKGIFTGALTTIAAFLSMVFVDSPGLQHIGLMTGLGISLF
                     FVSMVLLLPGWLVLFSTFMRIRTPKADRERKREGRFRGYYSRFSRWVILSTTLLAIPC
                     AVLTIINFIHFDYTPTKALPDEIESIKTLRALQKHGLGFSVDDSIVAFVEDPLKLYAI
                     EETLRKESKYIGSVVSLATYLPRSVIEDFDSFRQETVRVLNDAQNAFTLIALKRLNVY
                     DEIEEILGILVKSKNFAHFAELLFKSALFPPEVKDFFTMEIDGKLNFRVYAFTNTNIW
                     QHNRLKHYVEELNEIGFQFYGYPIIYYQITKKVISSIVFSVLVALVMIVVPVVVTLRD
                     FQLTFLALLALILNLVILFGLSYLFGTGLNFMTLLGLPLLLGMAIDAPVHLLLRYKDE
                     RSKGSQWHTDDFLKQVFLGTGKAITLSALTTAIAFFSFLIASSPLLLEFGVMLGTGIM
                     INWLLTFLWVGGIRIAIDQKRKTEGKQE"
     gene            complement(19619..20098)
                     /locus_tag="PKM49_00730"
     CDS             complement(19619..20098)
                     /locus_tag="PKM49_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81370.1"
                     /translation="MKDLFDALFFPKPFFSKPVSKLPVSIVAFLVVYYTNYCAYFPLF
                     PVSGNVLLFLFVSVAIAPFFLFFLMLGIDLVFAGYDQNRRLQNLYGFTFCPYLFLPFV
                     VAFARREGLFNQVFGFSLFAVLFLWSSLLTYRACEKKRVLLSKTIHDLVVLSFLLRF"
     gene            complement(20095..20994)
                     /locus_tag="PKM49_00735"
     CDS             complement(20095..20994)
                     /locus_tag="PKM49_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81371.1"
                     /translation="MKEVVLKLLAFSVGAVGIAYGVYGVFSYLAYSSVDRRSYTNPPV
                     LYFRQTASARALEPLYDLRVPYFEEKTDVLCRDDGVYEVATFRSFQQYTQDYWKGNPE
                     EKPEVTISRASFLGRDYVSLAVARDTRTLLYYRPKLLILVYGYGDDAAFLALEESDFP
                     LLRGITCSKKQVRETAEAFRKMEVPYCAITYSPTAPASLTGLLETALFPNQWILNKRK
                     PTLSPSGEASETRALRVKRLLYDAILNAIDSGFGMIELEAGYLEITTLNHILEGLEPL
                     PVSLTDLLFWSTEGFSSTGRVAS"
     gene            complement(21000..>21222)
                     /locus_tag="PKM49_00740"
     CDS             complement(21000..>21222)
                     /locus_tag="PKM49_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNY81372.1"
                     /translation="SNGGELWLTTAHYFTPKGNDIHLKGIEPDIVVEPSKEQLEAVSE
                     SNSTQYEIAVEPGKDIQLQTALAFLIDLL"
     assembly_gap    21223..21232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21233..22223)
                     /locus_tag="PKM49_00745"
     CDS             complement(<21233..22223)
                     /locus_tag="PKM49_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNY81373.1"
                     /translation="MRKLLKAALVISAVLVVVSFSWTLFAQTQNVNVYEKLEPFFESL
                     YFIEKDYYEKGTIDYDNLVNASIRGLLSGLKDPFTYYLTKKDVAENQIDQEGKYGGVG
                     LEVTYHAQLKVLQVVAPMYGTPAYKAGVKAGDLIITIDSAPVEEMTYMEAVNRLRGEP
                     GTLVTIQVFRENTGALEFIIGREEIHTAMVQYGTVDYSGQKIGYVRINSFFKNTATEL
                     HTALQKIFSKNPKALVLDLRNNPGGYLTQAILVCSMFLDSNEVIVTTRSSDGFINTYK
                     SIGNDYPNLPMALLVNGGSASSSEILAGCLKDYNLATLVGEKTFGKAAVQTLFP"
     gene            complement(22220..22585)
                     /locus_tag="PKM49_00750"
     CDS             complement(22220..22585)
                     /locus_tag="PKM49_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HNY81374.1"
                     /translation="MENERLKPIIVRLKKIEGQVRGLQRMIETDKPCASILAQVSAVQ
                     AALRMVGRKILEHYAYICLDDVQRLATQYQGAASEKEWEKSAPSSAMKEKLEALSELI
                     GQLMSSESLPQPLSEGEKE"
     gene            complement(22614..24269)
                     /locus_tag="PKM49_00755"
     CDS             complement(22614..24269)
                     /locus_tag="PKM49_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HNY81375.1"
                     /translation="MIRLSVINNQTEYTYETPVTGKALLKATGMEFPRPVLGARVNNR
                     IRELSSPIYIDSTVEFFDITSSDGFRMIQRGLTFLLYLACKETFPERKLRVLHSIKNS
                     LYCELGDWVSERDIQLLKEKMRGLVEQDLPFWKERLDKIVALKYFKSAGDADKVKVFK
                     FRKKSTVSICHCGDKPNINYFYGYLPPSTGCLSVFDLKAYSQGFLLIHPTPLSPDSLP
                     EYKELPKFSQVFLQYQRWGDILDVKNVGDLNESIVNGRIRELIMVSEALHEKKIAQIA
                     TEISRSANKRLITIAGPSSSGKTTTSKRLELQLKADGLFPISISLDDYFVAREATPRD
                     EKGEYDFEDILALDLELLDYHLCSLLEGKEVHLPKFDFVTGSSKPSGKVIRIDKSQPI
                     IIEGIHGLNDKLTASVPRETKYKIYVSALTQLGIDDINRIPTTDTRLIRRIVRDHHFR
                     GHSAMRTIKMWPSVRRGEEKFIFPFQEDADVLLSSALIYELAVLKIYAEPLLVQISEE
                     NYENTEAKRLLKFLEYFLPITDTHLIPPNSILREFIGGSIFHY"
     gene            24350..24425
                     /locus_tag="PKM49_00760"
     tRNA            24350..24425
                     /locus_tag="PKM49_00760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24383..24385,aa:Gln,seq:ttg)
     gene            complement(24467..>26345)
                     /locus_tag="PKM49_00765"
     CDS             complement(24467..>26345)
                     /locus_tag="PKM49_00765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY81376.1"
                     /translation="NRDEALAVLTKDGTDLKYDYWVFSNELSTTLQQLMAYYRQMGMD
                     PDELFEEPYVELNAIRSLVDGKVLSYYADQTHITPTKAEIDAGIQQVIEENITTDEIR
                     QAVIARYGSIEAYKELIRAPMRTQMTREKVNALVSQPTEEQYRAYFQDNLSSLQSSGN
                     QVKGAHILVDSETLASSLIERIQSGELTFTAAASDFSLDASNAPNGGDLGWFGKGQMV
                     KEFEDAAFAASPGDLVGPVKTDFGYHIIRIDDKKTLSSFEDFKGQSELWEKAKGTLET
                     ENYTKWLADYKAKEKIAYEINDNVLEIFDDYVKETNESESATAVVAFRSKLEPFILIP
                     IDGKVVWAQDVDPRVSALFLKILETENEELQKDVYLFDRAKLLQNSLNPSVAGLGMEE
                     ITQRLAELDAQLAETTDGASRTLLLNQKFDLKDVEEYLQKLDEIAAKGLVFSELNEAA
                     DKARAKILRNDELRLLGYKELYDYNTSSKAVVEKLHSMVPEDPQVSLRYYQSMLNEFL
                     PYVHNKSIYLQYRVYLEPQMKEIQANLTGLAQDGTAPTDTRVAAYESLLTLMESLENF
                     EEEVVYLKELKTLKPNYPDIDLIIKQVEEALEIARLQAEEALRDAASGKALTGEATGE
                     H"
BASE COUNT         6176 a         6918 c         6791 g         6450 t
ORIGIN      
        1 agaggggttc aacccggaag agatcgaaaa ggtcttcgga ggcaactacg cgcggttcct
       61 ccgcgctcac ctttcgcagg cataggatcc ggaatctcta aagccgggcc gttaaagccg
      121 cccgtcggac ggggaaggcc cttgaacggt catccaaaaa cagaccccgt acagtccggg
      181 tatgtgaaac agggggtgcc tgggcacacc gatccaatcg tcgcccagca tcttgtgtat
      241 ctccgtcgtc agtgatatat tgtgtacggg ggcgtcgcac cccgtttcgt cgtaccacaa
      301 ccccgtttgc aaacgtaaag aaaaatgacc gtcggaggaa aaatcgcctc cggtgggctg
      361 taacgcgata agcgctcggc ccgagttttc acgctgcgac tgcgacagca gctcggacag
      421 ggaatgctcg gacggttggt aggcgaggtg cgggtaatcc atagagggaa cggagtcata
      481 ttcggcttgg gccgcgccgg ttaaaggcaa aaaaaacctc gttgccgtca ttttatcggt
      541 aaaaggcatc cggaagacgc cgctttcgat cagaaggcac cggtcctcgg ggtttgtcgt
      601 cccttccata tcgaaaaagg cgttaaagaa ttggtctttc ctgtttctcg actgatagag
      661 tgtcaacccg tctgcgaaga ctttttcgcc cattctgccg gcgtataaag aagagccgga
      721 cccgtatgag ataccgttga gggtttccca aagcgtttta ttgtacagag ggtcgttgaa
      781 gaaaaagagg accggaatcc ttccttcggg agcgcagggc ttctgtccga gcccgtcgca
      841 gagcatccga agggcatcca acgccgcgcg cttgtcaaaa ctcggtaatc tgaaagaaag
      901 cgttccatct tttacgtttt cggagtcttt tctttttagg gttaggtaga attcaaagta
      961 cctggccttg tgatagaggt cgagcccgac tgtattcctg agatggcggc tttcgctgat
     1021 ataacggatg tcgtgaacga aagagcaatc gggaaatcgg cggttttgct ctcccagcat
     1081 ctcttcgacc tcccagagaa aatcgtacgc atccatatcc gtctcgatgt attcacgctc
     1141 ttcgtgtttg tccgcggtcg gggaggccgt gtacacgacg cccatatcca gacgtttttt
     1201 cgcttcttgg gttaaccctt cttcgtcgaa ttcgcccaac gcgccggcta taccgatctt
     1261 tccattctcg tacaaacgga cgctaccccg agtgatctct ctcgatcggg tggaatcgat
     1321 ggcaccccct cgcaatcggg cgcatatctc gcccacagtt tctacgtacg cttccctgat
     1381 catacgagat caacccacca catcgatctc ggaaatccgc acataggggc ctccgaaatc
     1441 gatcggcaat ctcaattggt ctctcttcga acacgtgacg aacggattct cgccgagact
     1501 cgctcgatcc gatatcccgt cgattttaaa tagggtgcca aaggaatttc cggttagcag
     1561 cgaaattttt accggctcgg cgatcgttcc ccttcgaatc cgataggcga tgatcggaga
     1621 aaccgtaaag ttggtggagc cgaagccact tttgatcgta tcgatgagaa agccattgtc
     1681 cacttcgccg atgagcgagc acagcggtgt ttttccgggt tcgatgtagg tgttggtcat
     1741 ccgaacgatc ggttcatact caaaactgat cgccctcgcg tttccggtga ggttttcacc
     1801 gaagtcgacg gctgtggatg cgttgtgcag cctcccagcc atcacaccgt cacggatcag
     1861 ataggtcttc cgggccttcg tcccctcatc gtcgaacggg gtgtagccca tccccaaaac
     1921 gccgccgtca tcgacgatcg acaggttttt gtgggcgact tgctttccga tcacccaccg
     1981 atcctttagt gtctggtctc cttggtaagt gtccgattcg ctgaggtgtc cgaagctttc
     2041 atgcgcgaaa acgcccgcgg cttgggggga gagaatgacc ggataacgac cggactcgac
     2101 gctcttcgcg cgtttgagga aatcggcgga acgattcagc gtgttcgcga acttccgatc
     2161 ttgagcttcc atatctcggt aaacgtgtgc gccatagcta aaggaatctt tgatctggtt
     2221 gcctttcgcg ctcatctcat aggaaaacag aaggcctatc ttttggtaat cgaaagagca
     2281 atccgcgcct ttggaagaaa taaaacgctt atttcgcaat tcatcgacat agcgcgccgt
     2341 ccagagccgg atatggtcgt agctgtcgat gatcgggaag tagtgagaga gcaggtcata
     2401 cttttctttt atcgttttcg aagaaacgga ctgggggcct gaaaattgtc ggagggtttc
     2461 ccgatgggct tccaacttct gcaccaccgg gtgttcggac acggcctgtg agggggtggt
     2521 taggctggcc aaccgatcga tgtgttcttg aacggcgtcc acggatgacg tggccgaata
     2581 gtaccaacgc ttgccgtcga aaacgcgtac aaatgcggct tcatagcgtc tttccagcat
     2641 ttcctggacg tcgccgaacc cgaaggcgat cctcgtttga aacacctctt cgattctgac
     2701 gtcgcaatac aacgaatcag gaaattcgaa catcccccac ctccctcttt cagttcacga
     2761 tggcttggaa tcaggactct ctttcattgt acaagtccag gcaaacccaa tgtcaagcag
     2821 aaatagccgg acatttgggt aaaaatttcg taaaacgtcg ggtttacccc atcttctgcc
     2881 ctttgtagac ccaaagcgaa acgatcgaat cctttcggaa acggtccaag agatcgggag
     2941 tgacctgaat ctccgccaga cgggaggatt cgatcttctc gagctctgag aaggccagtt
     3001 gtatccgttc cagatcgaac atacggcggt acttccggta agcgggcagc agctcggcct
     3061 cttcgggctc gaaatagaga taggagccga gccaatagcc ctcgtttttc aacaatggga
     3121 gcacgttttt cacggggtag aagtcattgt cgaaattatg agcgctggat ccgaacatat
     3181 cgaaaacaca atagacggac tctttgcgca agggaagctc gttgaagtcg gcgcagatga
     3241 agatcaaccg tttattcgga taatgtgtct cgaagtgctt cttcgcatat cggacgagcg
     3301 acaagtgatt ctccgccata atataggtga ctttgtccgg cagtctgtcg ataaatccca
     3361 tcaggaaaaa gcagaagcca gaacctatct ccaagacgct taacgggcgg ttttcgaaag
     3421 ccacgcgccg catcacccag tcaaaacctt ttttcgtgaa actgatgaaa agcgggtcgg
     3481 tgctatccag ataagcgcga acggggttct cctcttgcag gtaggagggg ccgccctccg
     3541 cgccggaaga gggcgtgata acgatgccgt cttcgatact ggcttgatag ccacaggagc
     3601 acaccaattc gccttgatag atcatattgc cctcaacgga agccttgccc acttccaacg
     3661 gctcggcaca ccgcggacag gcgaaggcgc tgaaaaagga tatcggaata cccaacggtc
     3721 tgtagacggt gagcttctga gtttgaagct gagcgatctc ttcgttaatg agcgatagcg
     3781 cctcatcgag agcagttttt tccagcagca gaccctctcg cttcttgatg aagtaagact
     3841 cgaagtagtc tctgtccttt tcgtcaacca attgcgttaa ccgatggaga gaaaagagca
     3901 tcttgatctc ggtgatcgtg aatcgcatct ttttgaaaac gaggattctt tctatatcct
     3961 cctcacaact gcgatcgaag gcgtactggt tatggatctt ttccggtaac aacaacccgt
     4021 tctcgatata ataccgaacg gtatcgatgg aaatatcgta cttctgagca aagtcaccta
     4081 tcctcatacg gaacccagcc tgcacctccg taaggggaac cgccaagact gacccccacg
     4141 tctctcacac aagcaacaaa aaaaaaccga cttcgcctaa tcggttcaac agacagcgcc
     4201 ctcgattata caacaaaacc gcgcgctttc gatacggtat ggggagcgcc gactgggcgc
     4261 gccgatcttt agattcgcgt gttttctggg aacgctgaac ttcagttcgg caggtgttaa
     4321 agcttaccag acgctctcaa aaggcagcgc tatctgcgga ctgccagatc gtttggtggg
     4381 tcaactcgcc ggtttcgtcg atgaggaggc gttcataaaa cgggggttgg atcaaaaacg
     4441 gtaagacttt ttggaagagc gattgcaccg ccccgtagta gccgtcttgc agcccgccta
     4501 agaggaaacg cgagaccttc aactccgaat cttcctcctg cagcgtcata cctaagcccc
     4561 cgtgccagtg gccgtgtaag aaataaacgg gttggtctcg cgtcggtgac gtttcaagta
     4621 gtctgacaac aagagagtac agcgatggac tgtgcgccaa gacgaacaga gcggtgacct
     4681 cgggcgcgga caaggcatcg gcaagccagc agtccagctc ggctctgtcc agcgtttggg
     4741 agaagtcaca caagaggatg aaccgtaaac gaacatcccc tttttgatag tcaacgatcc
     4801 ggttgaagaa agagcccgcg gtttcctgag tggatggaaa gaggaacagc caatccaact
     4861 cggtggtttc gtaggctgag ccgatatcgc ggttacccgg aacgaacagg gaaaggacag
     4921 aatcgggtat ccggtcccag taatgcgcga aacgatcgat ctccttcagc gcgagcgccc
     4981 gccctctttt accgtcgatc gcatccccgg tgtgaatcac gatgtccggt cttttgtcgt
     5041 tcacctcgtc gatgaaccac tccagtcgcg cttccaaggc ggaatcaccg tacttcaggt
     5101 gattgtcggt gacccagaag atgcggactg gttcggatgc gaacagaggg gatagacaaa
     5161 gaaaaaggag aagtacaaat agaaagaacc ggtatctctt ttgcgtcata ctcgactgat
     5221 aacctccatc aggaatggac aaagagcaga tcgcagggtc gaaaaaccac ttacgccgca
     5281 ataatgccat agacccatcg cggcgtaagc gtttcttttc tcgcgacggc gcatacgcgt
     5341 tcgagaatgg gaaagagcag atcgcgagac tgcgtgggct tccgaagtca gcccgctggt
     5401 taccccgcaa ttaacctctt agttatgtca ccccatagct tgaaccacac cggattttct
     5461 cttccattga ggtcaagaag acgctcgggg gaatctccta ctttttcgaa acctgcgcca
     5521 gatgaagtcg tacgagttgt tcctcgacta gaacggctat cttcccgtat ccactgtgtt
     5581 cctgtttcaa caggtatcga tcggggtcct ctatttcgtt accaatcggc catagattac
     5641 aaaaacgagt ttatggtgcg cagatcagcc tctatgcgat cagaacggca atagaaagcg
     5701 ggatgatcgg cttcatcttg cgttcgacca aacaaggtca atctctctac aaaagcttcg
     5761 gattcacgga gataaccgtg gtacgttacc tgttcctaaa catcgaatag acgttcttaa
     5821 gccattattc gattatgata acacgaagct cgccgagcga ataattctac tcaaaaagag
     5881 gttgcatatt tgggcactat tcagttgttt cccccgctgt aaccgttaat gcttgtaatg
     5941 gtttatgggg tattgttttt tattgaaaat ggctttcgaa cggataagac attgacacta
     6001 tccaacgtga cactataccg cgcgtagaat tgtcttgttg caccaggagt gtcggagtgt
     6061 cttctagcta taccttccgc ttcaattatc caaacgggat taccgtcagg atctgtatac
     6121 aatcggtgat tcgtaggttg tgagtcacac ccttcaaaca cgacagaacc gtataagtaa
     6181 ggctgaatat tgtctgttcc gaccgcagta ataaaatcag aaggcagagt gatcgagatt
     6241 tttcccgtaa tcctatcgcc ttcgttgacc aactcaggtg cgagcgagat gagactatcg
     6301 ttaatcgtca gagcttgtaa tatatcgaac tcagtcgttc ttgatcgttt taaatcccca
     6361 gaaaaagcga cgagttccaa gcgataattt cccgaagcgg cggtgtttga tgcaataacc
     6421 caatattccg taaagacaat gtaatctcca ttcatagaag tacttggcgg cgattcacgt
     6481 acgatatttg gaggtgcgat ttccgcattc gatacattcg taagcgtggc aagagtagat
     6541 ggtacgcgat acggattatt aaacgcatat tcgaatttga acctgacttt ttcatctctg
     6601 aacaacagag aacccccgtc ggcaatacta agatccctcg tttgcaaatc cccttgaacg
     6661 acgttgaact ttctttccgt tacgtaccct acatttgggt ttgcggaaga ggtcgcagtt
     6721 gccacgaggg tgaacgaagc gggttcagta aagactatcg gaggatcgcc ggaatatacg
     6781 atccaatcat cgttttctga aagaataagg tctcgggtta aaggtggctc tgatgacccc
     6841 tcttcccata aggctatcgt aacatgtgtt acgactggcg ggactcgtat gagtcgaacg
     6901 atcggcttta tcacttgccc gacaaacaac gggtctattg ttgaaggttg gatgatttca
     6961 acccgaggtg ttgaaggata cacatttaac tgttcgccag aagatgtcgc caacacggaa
     7021 ctcggattag tgttcagaca atagatagtc ggtgtcgccc aaatgctccc cacttttgag
     7081 aaaaccacct gatcgaaaag cgcgcttgcc ggtatcgtag tagacgaaag cgttggcgcg
     7141 gcaatataca ccgcaggatt aggattacct tctatttcta atcttacttg tataaggctg
     7201 ttgtctgaaa ccttttggtc gttattaaag acgacacgta attgttcttg ctcgtaaata
     7261 aagtaagtgc cgttagtaac atccgaaggg aaccgtaaat tcgaaaagga tggaagaacc
     7321 acatcgaagg tttcttgtcc ttttgcaata aaattaccgt ttcctgtagc ctttacttcg
     7381 gcggttaccg tagcggcttg ggtgcctttt agattgaaca aagcctgcac cggtagatcg
     7441 atcaggatac tgggttcgtt tgcggtaacg gttaagtcgt aggtacttag tgaacataag
     7501 tactcataag acccggtaaa gaattccgga atttgatcga tctctaatcg gactctgggt
     7561 tctttcgctt gtatgaaggc aacgttgcca accgaagtga ttctgacctg cgggttaaag
     7621 ggtaccacgg ttaacgtggc tcgcttcgta gtcaacgggt tagtcccaga gagatcggaa
     7681 gtatcgtaga ttgagatgac gaccaccgga ctgctcggaa tcgtcggcct gaatttgaaa
     7741 ttatagtact tcgccgaact gccactgacg tcagatgaat atggagtcgg gtcgatctct
     7801 cccaaattgc tattctcgag aaggactctt ggactttgta tgttcattcc ctgcgggatt
     7861 gtcacggtca cgcgcagcga cccttcgtaa ccggctaagt actgcttcga ggtatcgata
     7921 gagactccta ctgtcgaagg aggaatagcg taatcactgt cggaacgcat aactggtagg
     7981 tctatctgcg gtacctggtt caacgtgact gacgcgtaga ccgtaaaggt tcccggttga
     8041 ctgatataga acggtttatt gttcctgaat gtcgtattat tgttgttggt gggtgacgcc
     8101 ccaataggta caacaccggg aatagaaacc cccgtcgcgt cagtgatgta catacgaacc
     8161 gataggttcg agacgttgtt cgggttttcc aattccaata ggaaatacaa cggggctccg
     8221 ataaatagct ggcttggact gccttctagc gcgaaagtct tttttcggat ttccgcggac
     8281 aatactgtga aagtccggct gttgacaccg acagaaagcc cggctgtcgt cgcagtggcg
     8341 atgatggtat agactccctc cgtcacgaaa ggctcgatgg gatcggtata gcgtgtggcg
     8401 cgttgatgct caagatagac agcatctttg taaatctcgc tgcccttcca aatcgcataa
     8461 ctgaaagaat cgatcttgtc gctattcggg atgccatcca ctgagaataa cagagtgatt
     8521 ggccgtccca ccgtgatcct gtcgattgct ggagacgctt cgatactgat acttatatct
     8581 ggcgctttta cctctatggt aagtgttaag accttcgtgt tcccactgat agtgacagca
     8641 gttgcggtga tcgtgtaaga tccggcgaca ctgaacttag gaggagtatg ggtatacgta
     8701 aagcttccca tctgcgtggg cgccgtttcc gtctgatgga tagcagatcc ggagctgatg
     8761 ccgtaagtga aggatttgat cggatcgaca actgtggcgc ccgtcaccgt aaacacaagc
     8821 gagaaggctt ccccggttcg tatcttatcg atctctactg gagtagccga aatcccaaca
     8881 ttcagttgcg ctgcggccac cctcacctcc gtcgccaatt tggtttccgt cgcttggctg
     8941 ccgatcaccc cgctcaaacg aaaggagata tcgtataggc ccgcttcttt cggaatccat
     9001 ttttctttcg tgacgaaggt gtaggcatcg gacggagtgc gctgcaacgg ataggtttcc
     9061 gccacccctc tctcgtctga ggagcccctg cgcgcgatcg cgacctcgcc attgatgggt
     9121 atcgcagcgg gtaggtcggc gcccatatac acttctatgc gcgtcggatc ataggcgttc
     9181 ggaggtttcc cgctgtccgt gacgtatttg tacccagtta tgcgcaagtt cggggatctc
     9241 agggtgaagg tcgcggtcgt ggtgatctcc tccccgaagc tgtcccgtat cttcgcccgt
     9301 gctttcttta ttcctgggct catatcgagc gtcgatttgg tcgcgacata gatcacgctt
     9361 ccgccggctc cgatcgttct cacccagctc agcgcttcct cttcgacgct catctccaaa
     9421 accgttaacg aagcgttcgg gtttgccgtg tcgggagaga gcttcaaggt gacaggattt
     9481 ccgatatacg tctcttccgg ctcgaccgta aacaccatcg tcggttttgg agcatccaca
     9541 cggaaggtgc aagacttcgt gtattgatca tcaccccatt ggatgagcgc gatcgccgta
     9601 tgaactccgg ccaccgcggg gcgataggtc ccagtataga tgtagccggc ttgcgaaatg
     9661 ctaccgattt tgtacggggt tccaccgttt ggagccgtca ccgcgatctc caccaccggg
     9721 acgatccctc cgggattttg agagggaggt gtgaccgata ggaccaacga aacgctcgaa
     9781 ccgagcatca gcacattcgg gtaggcggag agcgcaccaa ccgtgggtct gacgatgccg
     9841 gtcggatcct tcacctggat agaagccgtc gcggtggcct gatttcccgc agtgtccgtg
     9901 gccgtcacct gtatcgtccg agtccccgtc ccttcgggcg tccatttcgc gaaatagaag
     9961 gcgttgtcat aggtcagtgg gttgagggtc aggttatcct caagaacgga aagaccaggg
    10021 ataccggcta agtcagaatc ggaatcttga accaaaaagg aaagatccgt ttgaacgttc
    10081 ttataggcga cgctgggcat atacagctgg atccttggcg gcgtcttgtc gatcttgtac
    10141 ccgatgacct cgatctcgtc actctgaccg gtagcgcccc agctatccgt ggcgatagcc
    10201 ttcaggtatt ttttcccgtt caacaccgtg taagtgaggg tttttttgta tgagctctct
    10261 ccaatacctg gaaaagcgct cgcagttgtg ttgttcacca aaaaccgcgc gttgtctatc
    10321 aatgcgccgt cataggtgtc aatcttcagc aagtaggatt tggactcgtc tattggaaaa
    10381 gaccccgcgg gtccttccaa ggttactttt ggagctcggt ggctctgaat cgagaggtct
    10441 acttcgtccg tggtgacgat attgttccca ttggtcacgg tgagcgtcac cttttcggat
    10501 ctcgtcgctt tcaagttgta acgaagagaa taggtgggga ccatcgggtc aaactgatag
    10561 gtctttttta tgccggagag ggatcgaagc tcgatccgct tgatgtagga gatgttgtcg
    10621 gtgatctgcg cgctcagggt gacgttctcg tctgtgaaag gggtcaattg tttcgggtga
    10681 ttccagccga tgcgcgcagg ctcgttgctc ctcgggggag tcacttccac atacttgttg
    10741 gagctgctgc cgacgtatcc gtaactgttt tgggcgaacc ctcggataat gtaggttccg
    10801 atggacaagg gcaatcgagt cgactgcctt tgtccttcca attcatactc cgtaccgttc
    10861 acggtgaccc aacggcgaat gaccgccgac tcggactcat cggcgattac ctcgaaggtg
    10921 taatcatttc cattttcgtt gacccgagac accttcacgg aaacgttagg cggtgtgaag
    10981 tcctcggttt cgatataggt ctctttggac ggccaccata ttgcgttccc gttttgccga
    11041 gttcttccgg gtgtttcgat aaaaaaaccg aactgcgaag agtggagttt gatagagaat
    11101 tcgtgagaac cgacgttctc ctgcatatac gatctttctt ctcccgtgac gaagtttttg
    11161 gtcgtcaaac ggatgtaggg gagctctttc ctcggagtcg tcgagacgag gactttcaac
    11221 acctgcccta tgtaggaagg ggtttcgggt gtcatcgtca ccttgatcct taccgatcga
    11281 gaggtccctc caaaaaggtc ttgtaatacg caagagctga atagcagcaa aaagaaaagt
    11341 atgataaaag ccaccatcgc ggtttttttc atcgttctca ctcctctctc gcgagacttc
    11401 ccggcgtccg gccctttctt acccgtctgt attatttaga cggtttctcg ccgaaaaaag
    11461 atctcgcgcg ccggttcgac accggcgttc acttccagaa cagaatcgag ctcgcgcgga
    11521 tgttaccgtt ttcgtcctgc gcctccaagt agaccttaat cttctctccg atttgtagtg
    11581 tcgggttccc accatacagc ggatcggaca agaaaaactg aaacttttgt tcgaaacgcg
    11641 ttccatttcc taaaggcagg aacgacacct gtaaagggta aggggtggcc gtcgtaccgg
    11701 acgccgtggc caccggctct ttgatcgcgt aataagtcgg tctgataatc tcttcccacg
    11761 tggtgcccaa actggtgtcg tcggtcaccg tcacgacgaa aggcaaagat ggatcatcga
    11821 cgaccagctc tgaaatagta atcaccggtg gtatatcgtc ttcccaaacc ggggttgcca
    11881 acaccgtgat cgctttccgc ccgaaacatt gtatcccctt gtgaatggcc gtcacggtga
    11941 tttcgtgatt tccgattcgc gcattggtgt acatcgactc caaagcgttc tgggggaact
    12001 gcgcgttcga ggggtagatg ttccaaaccg gcacggaatc gaaggtgtag tcggtgtcga
    12061 tcgtgaaacg aatcgtgtct cccacacggt attcagacaa caacgcattc ccgccgatct
    12121 tttcatcgat gatcttcaga gattcgtact gggtcacgcc caactgaaaa tagaccggcc
    12181 cgagtttggc cttattgccc atattgtccg aggcaacgac gtacaggctg tacacgccgt
    12241 cggggaaggc gttttgtcga agtcgaatcg ggatcgtcgc gttcatttcg atcagtgagt
    12301 cgcaggggta cacaccggaa agcttcttcc cttccccctc tacggtccag gaaagctctt
    12361 tcaaaccgct tgcctcgtag ttgggcttat ccgggaggtc tttcaccgac aggagtagat
    12421 agaactcatc catgacctta aaatcgaaaa agaagtctcc cggctcctgc ggttggaaat
    12481 cgtagtccaa aaaaacgggc ggcgtcgtgt ccggataggc cgagctgatc gtcgatgtcg
    12541 cgaagacgaa gtcctgctca tcgtcctgat atcggcccat caccatacgc agctcgttct
    12601 cccccttttt catcccttcg agcgctacgt tggcgataca gatcaaacgg tttttcgcgc
    12661 ccgtcgtgaa gatttcgtgc ggcgcatacc agaaggtaga tgtgatgtct ttcccgttta
    12721 aatagaaacg ggttttttct cgcaacgcgc cgaccgtgtc gtcgttgaag atgggagatt
    12781 gcgcatccca cagctgcatc caaaggtacg ccaggttctt ctcgaatgga atcaaggtgg
    12841 cttcgtctgt cgtgtaaaac tctatccttt cgaggacggg tggtgtggga tcagcgattt
    12901 tcaaagagcg ttcgtatgtt cgaacgttct tatagaagtc cgtcaccgtc gcatagaacg
    12961 agaaatgtcc ggcgttgtct tcggaataga aaaggcaggt cgccgtccgt gtgttggccg
    13021 cttcggttcc caaccaatag gcattgtcct ccgtccgaaa ggcggacaat tccagcttga
    13081 tcaccggcaa ccgccctttg taggcgaaat ctacgacaaa agacgccggc gcagtgaacg
    13141 gccccgatag gggcctaccg gtctcgggat ccaaaaactt gaatattccg tatttctcgg
    13201 cattaccggt cgattgaccg cttaatcccc tccccgtaag caacacgcaa gaggtcataa
    13261 ccagaacgag tatgagaaaa accgacgacc aaaaaacagg acgccaacct ttccgtttgg
    13321 cttcgtacat cgcaaccacc tcgttctatt tttctgcgac ttccggcatt gtcccttatt
    13381 ttatcggcag aatccctttg attataaagg aacgccggtt agaatcccct tagataccct
    13441 gtaggtgatt accctcgcgg ggatcgcggc gtaagcgctc cttcttttcg cgacggcgcg
    13501 atacgctttt cacaggcgaa tcgttgggct tcattagtat cgcggggtcc cttcggttac
    13561 cccgcgataa cctcgctcgg gcatcgcggc gcccctacag ttatcgcggc gtaagcgctc
    13621 tttctttttg cgacggcgcg ataccccgac ttcgtcgctg tgtaaagaga acaaaagggg
    13681 gatcgcgggg tcccttcggt taccccgcga taacctcgct cgggtgtcgc gagacttcgt
    13741 gggcttccga agtcagcccg ctggttaccc cgcgataacc ccgttcgggc atcgcggcgc
    13801 ccctacagtt atcgcggcgt aagcgctctt tctttttgcg acggcgcgat accccgactt
    13861 cgtcgctgtg taaagagaac aaaaggggga tcgcggggtc ccttcggtta ccccgcgata
    13921 acctcgctcg ggtgtcgcga gacttcgtgg gcttccgaag tcagcccgct cgttactccg
    13981 cgataaccca caccgacatc gcggcgcaag cgctcctgct tttcgcgaag gcgcgatacc
    14041 ccgacttcgt cgctgtgtaa agagaacata agggggatcg cggggtccct tcggttaccc
    14101 cgcgataacc tcgctcgggt gtcgcgagac ttcgcgggct tccgaagtca gcccgctggt
    14161 taccccgcga taaccttcac ggggatcgcg tggtagcgtt ggttacccgc gatgacccac
    14221 gcggttattt ttcgaagaat tttttcaagg ccgctttgat gagttcttcc ataccccact
    14281 gctttttctc cgagaagagt tctctcagca cccggcggga ttgaacgggg gaatacccca
    14341 gcgcttccag cgcttcggtc gcctgctgga aaagggagag gtccaaagcg tcgccttcgg
    14401 cctgcgtctc ttccaggagc gggaggacct gtttcacttt agatttcaac tccaacacga
    14461 tgcgctccgc ggtctttttc ccaacgttcg gcagcgtgct gagataggtc agatcgtttt
    14521 ccaagatact ccgggcgatc tcgcgaacgg aggcgtcgta gaggattttc atcgccgtct
    14581 tcgcaccgag cccgttgacc gtcgtcaaca gctgaaaaaa ggctctcttc tctctcttta
    14641 aaaaaccgta catacgccat tcgctttcgg aaggctggaa tatcacgaac gccttcactt
    14701 ccacatcacc ggtatcgtag agctctttat ccgcccatat ctcgaagccg atgtcgtggt
    14761 tctcgaggat gatcaagcct ccctctatgc gttcgacgat ctttcctttg atggaatgta
    14821 tcataatgag ccctttcttt tctcgttttt gtaaggggag gtaacttttg cgcgactaac
    14881 cgaaacaccg cgctgcctta ctatatctac gcccttcgaa aaacgagtcg cttattcggt
    14941 aaccggcttt ttgacagcga agaaccaaga gtagggtggg aaatagagat gcccctcggc
    15001 gaccccgagc aggtttgtcc ccgcggtcag cgagttcgcg tagatttccc actctccgtc
    15061 gggaattttc tggtcgcgct ccgaattccc caggtgaacg ccgatcagga gccacccctc
    15121 cgtctttttt cctttgagca cgtagaagaa accgtctctc catctttcta tggagtacac
    15181 gtttcgggcg atggcttttc cgcttttcgg tcttaggagc tcatatgttt ttcggagcac
    15241 caagagcccc cgcaggtagt gaaatacctc cgcaaaggtg tctttccgcc cccaatcgat
    15301 ggcgttgtaa aggtcccccg attgatacga gttcgattga ccgtatttcg tccgacagaa
    15361 ttcctcgcca ctgtgaaaga aggggatgcc atgagacata gccacaagga agtggcaaag
    15421 ttttacacgt gtcaccaatt ccggaataga gaggtccggg cggctcttgc gcagtttgtc
    15481 gaaaagggtg agattgtcat ggcaagaatc gtacataacc gttttccaag ggtcgctata
    15541 ggacttttct tcccagcttt ccatagacag catccctgtc aacacctcat ccaatcggtc
    15601 aaaccgacct gtgacgaaac cggtcgattc gtcgtccggg tagcctttta tcgcgtcacg
    15661 aaatcgatcg ttgaaaacgg cgattggcgc ctctttttcg aaatctcccg tactgcgttc
    15721 taaagggtgc aaaggggtgc tcccccctgt ccagggttcc ccgtacaata aaagatgagg
    15781 atacttcaag cgcaatccgc tgatcgcgct ttgcatagtg ccacggtcga tcaacgccat
    15841 caagtcaaat cgaaaaccgt cgacatgata ggtctctacc caatagcgta aggtttcgct
    15901 aaaataatgc tgaaacgcgg ggtgtttggt gtcgaattca tttccacagc ctgaaccgtt
    15961 ggaaaaggaa ccgtcaatgt tcagacgaaa gagggcctcg gaaaataaag gcgtaagaga
    16021 aaagccgttc ggttcccaga cgtggttgta cacgacgtcc ataatgacgc cgataccgcg
    16081 gctgtggagg gcttgcacca attgcttcat ttccagaatc cgtcgcgcgg ggtcccgcgg
    16141 atcgagactg taggacccct ccggcacggt ataacacaaa ggatcgtatc cccaattgta
    16201 gtcggctcga tgggtgtcgt ccaccgatgc gaagtcctgt accggtaaga gatggacatg
    16261 ggtgatcccc agctccacga ggtggtctaa acctgccgta tggccctcag gggtctttga
    16321 gccgccgaag gtccacgcca cgtaagagcc cttggcctcc aagtcggtcc tcggatcgac
    16381 ggtaaaatct cggacgtggg actcatagat aacggcgtcg ttgtatttgg gtaaaaacac
    16441 gtgcgaatca gtctcccaac ccggcgggtc caaatagcga cgatccaata ggacggagtg
    16501 ccgcgagttt gtcgtgaccg agatcgagaa ggggtccaag atttcgtgag tggctccccc
    16561 cgactcgacc aggtatttgt agatcgtccc gtgcaaattg cctgaaaccc gcaatctcca
    16621 gactccttgc agttcgtctt ccgtatctcg tttcatcgag agcagccagg agggtttgag
    16681 cgcttccggt ctatcgtaaa acaacaggct gaccttcttc gcgaaagggg cccataaccg
    16741 gaactggctc tcggtttcgg tacactttgg aaaacagttt tcccaagaag agaatggggt
    16801 tccggcctcg tgataatcgg aaaggagtgt ccggaatagc gcaggcgttt tctgagaaac
    16861 ggtgtggccg ttccacagga acgaaattct tagattcgcc gtgagcgtgt cgacacggga
    16921 ggaggaaaac cgaatgctcc aaagctccca tccctctttc catccgaatt cacgcgccca
    16981 cctcgagggg tcgattctct cgaccgactc gattttcgct cccacgtggt cgaccgaaag
    17041 ctcaaaatga tcgggagaca ggtcccgata ggtgttggta acgctcaccc atagctcgtg
    17101 cttttgaacc agcaaagcca agccgatcat tcttgtttcc cttctgtttt ccgtttttgg
    17161 tctatcgcga tacgaatacc acccacccat aggaaagtga gcagccaatt gatcataatg
    17221 cccgttccca acatcacgcc gaactccaac agcagtgggg atgaggcgat cagaaagctg
    17281 aaaaaggcga tcgcagtggt cagagcgctc agggtgatcg ccttcccggt tccgaggaaa
    17341 acctgtttca gaaagtcatc cgtatgccat tgcgaccctt tcgaacgctc atctttgtat
    17401 cggagcaaca gatgaacggg cgcgtcgatc gccatcccca acaacaacgg tagtcccaac
    17461 agggtcataa aattgagtcc ggtgccaaaa agataactca gtccgaagag gatcaccagg
    17521 ttgaggatca gggccaggag ggccaggaag gtcagctgga aatcacgtag cgtcacgact
    17581 accggtacca cgatcatcac caacgcgacg agaacggaga aaactatcga ggagatcacc
    17641 tttttcgtga tctggtagta gatgatcgga tacccataaa actggaaacc tatttcgttg
    17701 agctcttcca cgtaatgctt cagacggttg tgttgccata tattggtgtt cgtgaaggcg
    17761 taaacgcgga aattcagttt cccgtcgatc tccatcgtaa aaaaatcttt gacctcagga
    17821 gggaatagcg cgcttttgaa caggagttcg gcgaaatgcg cgaagttttt gctttttacg
    17881 aggatgccca ggatctcctc tatttcgtca tagacgttca gccgtttcaa cgcgatcaag
    17941 gtgaaggcgt tttgcgcgtc gttgagaact cggaccgttt cctggcggaa ggagtcgaag
    18001 tcttctatga ccgatctcgg caaataggtg gccaaagaaa ccaccgatcc gatgtacttc
    18061 gattcctttc tcaaagtctc ctcgatcgcg tatagtttga gcggatcctc cacgaacgcg
    18121 acgatcgagt cgtccaccga aaaccccaaa ccgtgctttt gaagagcccg taaggttttg
    18181 atgctttcga tctcatcagg gagcgccttc gtcggcgtat agtcgaagtg gatgaaatta
    18241 attatcgtaa gcacggcgca cgggatggcg agaagggtcg ttgacaggat aacccaccga
    18301 gaaaaacggg aataatagcc acggaagcgc ccttctcgtt ttctctcacg gtccgctttt
    18361 ggcgtacgaa tccgcatgaa cgtagaaaac aacaccaacc atccgggcaa caataggacc
    18421 atggacacga aaaagagaga gatgccgagg ccggtcatca gcccgatatg ctgcaatccg
    18481 ggagaatcca cgaagaccat cgacaagaag gcggcgatgg tcgtcaaggc gccggtgaaa
    18541 atgccctttc ccgcgctttg gatggcatat tggaccgctt cacgcatcgc ctcctgcttc
    18601 ttctttctgc taagcgcgtt gaagccggga taattttcta ataggtattc catcgagcga
    18661 ttcgaaaggt gaatcccgaa gtcgatccca agccccagca aaagcgccag gacgaagctg
    18721 gatgtgaact ctagactctg aaagatcaga tagaaggcgc cgagagagaa caccgatccg
    18781 gtaaccagcg tcaggaagat gccgagggtg atgctgatgt tcccatagga gacgagaaac
    18841 aagacggtga tcagacccaa cgtcaatagg gtggtcaccg aaaaatcgcc aaagatgatt
    18901 ctttgcgagt ccagggcgcc ttggtggctc ccggagaatc cgaaagagaa cccataaggc
    18961 ttcaggatgg cgccgacctt ttctttcagg agggacaccg ccctttgcgt gaaggccaaa
    19021 tccagggcgg ggcgcgtcaa cacgaccgtc atagtgagta tctggctatc gtcgctgatt
    19081 aacgcgtact tgtccagaga ctctatcctt tccatctccc tgagcaggtc catcaccttg
    19141 ttcatagaga tgcccatctg ccggataatg ccgaagtcca ccgcagcggg gtttgcaatc
    19201 agaaaattga gaaaattgag ggtggaagag agcttttcga agaggttggc gtgggtcaac
    19261 ataattcccg ccggacccat taaggaaatc gtgtccagct tccgaaaact cttgacgatg
    19321 ccggtcagct tttccagttc gttctttatc tgtgcagccg cttccatcgc ctcgaaggtc
    19381 ctgccgtcga tatcgacgac gatcatcagc gtttcgctga cggtctctcc cgtgaagagt
    19441 tccttgtatc tttggctgta agggtcatta tgagggagta gaaccaagag gtccgttttg
    19501 atagacatct gcagagaaag gtaggtcatc aggatcgaaa cgagcacgcc gaccagcaag
    19561 atttctttcc agaatctgta gacgaatttg gataaaagac caaacacgag gttcccccct
    19621 aaaaccgcaa cagaaaagat aaaacgacga ggtcgtgaat cgttttcgac aataggactc
    19681 gctttttttc acaggcgcga taggtcaaca gcgacgacca caagaacaac accgcgaaaa
    19741 gtgagaaacc gaaaacctgg ttaaacagcc cttcccgcct ggcgaacgcg acaacgaaag
    19801 ggaggaacag ataggggcaa aaggtgaacc cgtagaggtt ttgcagccta cgattctgat
    19861 cgtaaccggc aaacaccagg tctatgccga gcatcaggaa aaacaggaaa aaaggcgcga
    19921 tcgccaccga aacgaagaga aagagcaaaa cgttgcccga aacaggaaac aacgggaaat
    19981 aggcgcaata gttcgtgtaa tagaccacga ggaacgcgac gatagaaaca ggtagtttcg
    20041 aaacgggttt cgaaaaaaac ggtttcggaa agaagagcgc gtcgaataga tccttcatga
    20101 ggctacccga ccggttgacg agaacccctc tgtggaccaa aacagcaggt ccgtcagaga
    20161 aacgggcaga ggctcgagcc cctccaagat atgattgagc gtggtgatct ccaaatagcc
    20221 cgcttccagc tcgatcattc caaatccaga atctatggcg ttgagaatgg cgtcgtagag
    20281 caagcgcttc acgcgcagcg cccttgtttc ggaggcctcg cccgagggag aaagggtcgg
    20341 ttttcgtttg ttcaaaatcc actggttagg aaacagcgcg gtttcaagca ggccggtcaa
    20401 actcgcgggc gcggtcggcg aataggtgat cgcacagtaa ggcacctcca tcttccgaaa
    20461 cgcttcggcg gtctcccgaa cctgtttttt cgagcaggtg atgccccgta gaagcggaaa
    20521 gtcgctctcc tccaacgcta aaaatgccgc atcgtctccg tatccgtaga ccaagatcag
    20581 cagcttcgga cggtagtata gcagcgttcg cgtgtccctc gcaacggcca aggaaacgta
    20641 gtctcttccc aggaagctgg ccctcgaaat cgtcacttcc ggtttctcct ccggattgcc
    20701 cttccaatag tcttgagtgt attgctgaaa ggatcggaag gtggccacct cgtacacacc
    20761 gtcgtctcta cacaagacat ccgtcttttc ctcaaagtac ggaacacgca gatcgtaaag
    20821 gggttccagc gcccgggcgc tcgcggtttg cctaaaataa aggacgggag gattggtata
    20881 gctccggcgg tccacggacg aataggccaa atacgagaag accccgtaga cgccgtaagc
    20941 aatgccaacc gctcccaccg agaaggccaa gagctttaga acgacctctt tcatcggtac
    21001 tacaacagat cgatcaaaaa ggccaacgcg gtttgcagct ggatgtcttt gcccggctca
    21061 accgcgatct cgtattgtgt gctgttgctt tcggaaactg cttccaattg ttcctttgag
    21121 ggttctacta cgatatctgg ctcgatccct ttcaggtgta tgtcgttacc cttcggtgtg
    21181 aaataatggg ccgtcgtcaa ccataactcg cccccgttag acnnnnnnnn nngggggaag
    21241 agcgtctgca ctgccgcctt tccgaatgtc ttttctccca cgagggtggc cagattgtag
    21301 tccttcaagc acccggcgag gatttcagag gacgaggcgc tgccgccgtt gacgagcaag
    21361 gccatcggaa ggttcgggta atcgtttccg atcgacttgt aggtgttgat gaacccgtcc
    21421 gaagacctcg tggtcacgat cacctcgttt gaatcgagaa acatcgagca gacgaggatc
    21481 gcttgggtca ggtagccacc gggattgttc cgaaggtcca acaccagagc tttcgggttt
    21541 ttagaaaata tcttctgcaa tgcagtgtgc agctccgtcg ccgtgttttt gaagaagctg
    21601 ttgatccgta cgtacccgat tttttggcct gaatagtcga cagtgccgta ttgtaccatc
    21661 gcggtgtgta tctcttcacg gcctatgata aactccaagg cccccgtgtt ttccctaaaa
    21721 acctgaatag tcacgagcgt gccaggttcc ccacgcaatc gattgaccgc ttccatatag
    21781 gtcatctcct cgactggggc ggaatcgatg gtgatgatca ggtctccggc ttttacgccg
    21841 gctttataag cgggggtgcc gtacatcggc gcaaccacct gaaggacctt caattgcgcg
    21901 tggtaggtca cttccaaacc gacgccgccg tactttccct cttgatcgat ctggttttcg
    21961 gcgacatctt tcttggtgag gtagtaggta aacgggtcct tcagcccgct gagcaaccct
    22021 cggatactgg cgttgaccaa attatcgtaa tcgatggtac ctttctcata gtagtccttt
    22081 tcgataaagt agagcgattc aaaaaacggt tcgagttttt cgtagacgtt gacgttttgt
    22141 gtttgcgcga acagcgtcca cgaaaaagag acaacgacca ataccgccga aatgaccaat
    22201 gccgctttta acaattttct cattcttttt ccccctcaga taaaggttgt ggaagtgact
    22261 ccgaactcat cagctgaccg atcaactcgg atagagcctc caatttttcc ttcattgccg
    22321 acgatggcgc gctcttttcc cattcttttt cggacgccgc cccttgatac tgggtagcga
    22381 gcctttgtac atcgtccaga catatatagg cgtaatgctc gagtatcttt cggcccacca
    22441 ttctcaatgc ggcttgaaca gctgatacct gggccaaaat cgaggcgcag ggcttgtctg
    22501 tctctatcat cctttggagg ccgcgcacct gaccttcaat tttctttagg cgtacaataa
    22561 tgggcttaag acgttcgttt tccataaggt gttatcctcc gtgataccga tactcaataa
    22621 tggaaaatag agccgccgat gaactctctc aagatcgaat tcggagggat caaatgcgta
    22681 tcggtgatcg gtagaaaata ctccaaaaat tttaaaagac gcttcgcttc ggtattttcg
    22741 tagttttcct cactgatttg gaccaaaagc ggctcagcgt atatctttag aacggcgagc
    22801 tcataaatta aagcagagct gagcaagacg tccgcgtctt cttgaaacgg gaagatgaac
    22861 ttctcctctc cccgccggac ggagggccac atctttatcg ttcgcatcgc gctgtgacca
    22921 cggaagtgat ggtcgcgaac gatacgtcgg atcagtcggg tatccgtcgt tgggattcgg
    22981 ttgatatcat cgatcccgag ctgtgtgagc gcgctgacgt agatcttgta cttggtctcc
    23041 ctcggaacgc tcgcggtgag cttgtcattc aatccgtgta tgccctctat gatgatcggt
    23101 tggcttttat cgatccgaat caccttgcca ctcggtttcg aagaaccggt gacgaagtcg
    23161 aactttggca aatggacctc tttcccctcc agtaagctgc aaaggtgata gtccaacaac
    23221 tcgaggtcca aggcgagtat gtcctcaaaa tcatattccc ctttttcgtc tcggggtgtg
    23281 gcctctcgcg cgacaaaata gtcgtccaac gagatcgata tcggaaacag gccatcagcc
    23341 ttcaattgca gctccaatcg cttcgaggtg gtcgttttac cggaggagga tggacccgcg
    23401 atcgtgatca atcgtttatt ggcagaacga gagatctccg tcgcgatctg cgcgattttc
    23461 ttttcgtgca gggcctcgga gaccatgatc aattctcgta tgcgaccgtt cacgatagat
    23521 tcgttgaggt cgcccacgtt tttaacatcg aggatgtctc cccatctctg atactgcaag
    23581 aagacctgtg aaaactttgg caattccttg tactccggaa ggctgtccgg agagagcggt
    23641 gtcggatgga tcagcagaaa gccttggctg taggccttca gatcgaaaac gctcaaacac
    23701 ccggtgctcg ggggcaaata cccgtagaag taattgatgt tcggcttatc cccgcaatgg
    23761 cagatgctga ccgtgctctt tttccggaac ttgaaaacct tcaccttatc cgcgtcgccg
    23821 gccgacttga aatatttcaa agcgacgatc ttatcgagtc tctccttcca gaacgggagg
    23881 tcttgttcga cgagcccacg catcttttcc ttcaacagtt ggatgtccct ttcggacacc
    23941 cagtccccta attcgcagta taggctattt ttgatgctgt gcaacacccg tagtttccgc
    24001 tccggaaagg tttccttgca cgccaagtag agcagaaagg tcaatcccct ctggatcata
    24061 cggaaaccgt ccgaggaggt gatatcgaaa aactcgacgg tcgagtcgat atagatagga
    24121 gaggaaagct ctcgtattcg gttgttcacg cgagcgccca aaaccgggcg cggaaactcc
    24181 atcccagttg ccttcaacaa agccttaccc gtgaccggtg tttcataggt gtattcggtt
    24241 tggttattga taacggagag acgaatcaaa cacgctcaca tccacatcat attttcgttc
    24301 gcttcggttc ttgacaaatt ttcctgaata aggtaaaata cctcagtttt gggacgtcgc
    24361 caagctggta aggcatcgga atttggctcc gacattcggg agttcgaatc tctccgtccc
    24421 agccacgaaa aacccgggaa atccctttcc cgggtttttt taatggttag tgttcccctg
    24481 tcgcttcgcc ggtgagcgcc ttgcccgaag ccgcgtccct aagagcctct tccgcctgta
    24541 ggcgcgcgat ctcgagggct tcttccacct gcttgatgat gagatcgata tccgggtagt
    24601 tcggtttcag cgtcttcagc tctttgaggt agaccacctc ttcttcaaag ttctccaaag
    24661 actccatcag ggtgagcaag gattcgtaag ccgcgacgcg agtatcggtt ggagccgtcc
    24721 catcctgggc caaaccggtc aaatttgcct gtatctcctt catctgaggc tcgaggtata
    24781 cacggtactg caggtagata ctcttgttat ggacataggg caaaaattcg ttgagcatac
    24841 tctggtagta tcgcagagaa acctgcgggt cttctggcac catagaatgg agcttttcca
    24901 caacggcctt cgaagaggta ttgtaatcgt acagctcttt atagcctaat aacctcagct
    24961 cgtcgtttcg taggatcttc gcccgggcct tatcggccgc ctcgttgagt tcggagaaaa
    25021 cgagtccctt ggccgcgatc tcatccaatt tctgcaagta ctcctcgacg tctttcaaat
    25081 cgaacttttg attaagcaag agagtgcgcg acgccccatc cgtcgtctcc gccaactgcg
    25141 cgtctaactc tgcgaggcgt tgggtgatct cttccattcc caggcctgct acgctcgggt
    25201 tcaagctgtt ttgcaacaat ttggcgcgat cgaataggta aacgtccttt tgcagctctt
    25261 cgttttcggt ttccaggatc ttcaggaaca aagccgagac gcgcgggtcc acgtcctgtg
    25321 cccagacgac ctttccatcg atcggaatca agatgaaggg ttccaatttg gagcggaaag
    25381 cgactaccgc cgtggcgctt tcgctttcgt tcgtttcttt cacataatcg tcgaagatct
    25441 ccagaacgtt gtcgttgatc tcgtaagcga tcttctcttt cgccttgtag tcggccagcc
    25501 atttcgtgta gttctcggtc tccaaagtac ctttggcctt ttcccacagc tcgctctgac
    25561 ccttgaaatc ctcaaaggaa gagagggttt ttttgtcgtc aatccggatg atatgataac
    25621 caaagtccgt cttcaccgga cctaccaagt ccccgggaga cgcggcgaag gcggcgtctt
    25681 cgaactcttt taccatctgt ccttttccaa accaacccaa atcaccgccg ttaggggcat
    25741 ttgaggcgtc caatgaaaaa tcgctcgccg cggcggtgaa cgtcagttcc ccggactgga
    25801 tgcgctcgat cagggagctc gccaaagttt cactgtccac taaaatgtgg gcgcctttca
    25861 cctggttccc gctgctttgt agcgaggaca ggttgtcctg gaaataggcc cggtattgtt
    25921 cttctgttgg ttgcgaaacg agggcgttga ccttttcgcg cgtcatctgg gtcctcatcg
    25981 gcgcgcgtat gagctctttg taggcttcga tgctcccata ccgggcgatc acggcttgac
    26041 gtatctcgtc ggtcgtgatg ttttcttcga taacctgttg tatgccggcg tcgatctccg
    26101 ctttcgtcgg ggtgatatgc gtctggtcgg cgtaataaga caggaccttt ccgtcgacca
    26161 aactccgaat ggcgttcaac tccacgtacg gttcttcgaa taactcatcg ggatccattc
    26221 ccatctgtct ataataagcc attagctgtt gaagtgtcgt cgacagctcg ttggagaata
    26281 cccaatagtc gtacttgagg tccgtaccgt ctttcgttaa gacggccagc gcctcgtctc
    26341 tgttg
//
LOCUS       DAOPXN010000006        24815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_1979_length_24815_cov_5.819669, whole genome shotgun sequence.
ACCESSION   DAOPXN010000006 DAOPXN010000000
VERSION     DAOPXN010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 24815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24815
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1979_length_24815_cov_5.819669"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            511..840
                     /locus_tag="PKM49_00770"
     CDS             511..840
                     /locus_tag="PKM49_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNY81377.1"
                     /translation="MSENIGRGALTEAVFYILLSLMTPMHGYGIMQNVSQLSKGRLAL
                     GAGTLYGAINTLLEKGWIRSVPGEADSRKKEYIISDLGKNALENELVRLQELLQNGAA
                     IMGGRIS"
     gene            837..1391
                     /locus_tag="PKM49_00775"
     CDS             837..1391
                     /locus_tag="PKM49_00775"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HNY81378.1"
                     /translation="MKRAIWRLYLNFEKEEKWLNEMATAGWMLTDYSWCRYVFEEGRE
                     GEYVYRIELLKNLPTYPLSQKYLRFLEEAGIEYVAYYARWVYLRKKTADGPFDLYTDL
                     ESKIAHYRRVLRYWHVFAGIEMTIGLSSCAPALNAWWRGGVLSYSLWMSVIPFAFGIL
                     FLFIAAPIKRQLKRWKKELQIRES"
     gene            1572..2204
                     /locus_tag="PKM49_00780"
     CDS             1572..2204
                     /locus_tag="PKM49_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY81379.1"
                     /translation="MHPLYPSNVKLIEKIPYCEALFREKDILCRFKLNDDPDLTELDT
                     ALAAQGYTLDAPALLMHRPKGCDIPREQGNIEVSDSFNPEWMRFFVSAKGLDDRDART
                     FEYMLRHILPERRFILKTEGGLTVGCVLAVYERGFTGLFDLYVDEAHRGKGLGLSLVQ
                     QALVEGARINCPEAYLQVDETNERAIRLYRGMGFEVAYRYWYRYSAALKL"
     gene            complement(2180..3364)
                     /locus_tag="PKM49_00785"
     CDS             complement(2180..3364)
                     /locus_tag="PKM49_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNY81380.1"
                     /translation="MKEKNDDHSIPTIRLKGQFATALVYARTVDENAITQIVSLCNQP
                     FSEGTHIRVMPDVHSGAGCVIGLTMDLNRSDAVCPNLVGVDIGCGVRVSELGVTPINL
                     QQLDKDIRESVPSGQNVHRHTFPEDRDFFYSLFNDLIIKDQIKSREKMGWLERSLGTL
                     GGGNHFIEVDVDEEGRSYLTVHTGSRHFGLTVAQLYQAKAASKMQQNGEARRSRAKGT
                     PKDLAYLTGSDRNNYLHDIRLIQTYAKRNRERIAERILQKMGLKELASFESVHNYIDE
                     KNILRKGAIAAHVGQPVIIPLNMRDGCILGTGKGNPEWNWSAPHGAGRLMSRREAKRI
                     LSIDEFRDQMKGVFSTCVDRTTIDESPMVYKAMEEILPVLGQTVDVRRIIRPIYNFKA
                     AE"
     gene            4272..5309
                     /locus_tag="PKM49_00790"
     CDS             4272..5309
                     /locus_tag="PKM49_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY81381.1"
                     /translation="MMIKPKKLKRGDTIAVLSPSSGAPQKFPHIFEHGLSILEREFGF
                     RVKEYPTTRMSSKALYENPKARAEDMNRAFLDPEVKGIICSIGGDDSVRILRYLDTES
                     ILKNRKLLMGYSDSTTFLAYLNKLGLVTYYGPSIMSGFSYLNCFPEALEEYKRVFFEN
                     APYEIQPFSVWADAYRRWEYPENTGKVERMREDHTHHVWINKGAVSTGCLWGGCLEVL
                     DMINGTFVWPEKAFWEGRVLMIETSEDKPSPRQVGYRLRNYGAQGILSSIQGLLIGKP
                     KGYTVEEMRALNEEVKKVVVGEYGCDGLNIVGNIGFGHTDPHHILPMGVPLTLDPENE
                     TLTFVEPLFSS"
     gene            5680..6687
                     /gene="asnA"
                     /locus_tag="PKM49_00795"
     CDS             5680..6687
                     /gene="asnA"
                     /locus_tag="PKM49_00795"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HNY81382.1"
                     /translation="MKLPKGYKSKLNLRDTESAIKKFKDFFERALSYELHLMRVSAPL
                     FVKPESGLNDNLNGVEQPVGFVIPDAGHCQAEIVQSLAKWKRMALKRYGFKIGEGLYT
                     DMNAIRKDEALDNLHSIYVDQWDWEKIIPKKDRTLEVLKETVKSIYRVFKNTERFIAF
                     EYKVLEESQSLPDDIAFITTQELEDLFPDLTPKEREHEIAKEKKALFLLKIGGPLKSG
                     KPHDGRAPDYDDWELNGDILFWYPLLETAFEVSSMGIRVDEEGLVKQLKVAGCEQRLS
                     LPFHRALLDRQLPYTIGGGIGQSRICMFFLQKAHIGEVQASIWPEETHQVCSASGICL
                     L"
     gene            complement(6785..7876)
                     /gene="nagA"
                     /locus_tag="PKM49_00800"
     CDS             complement(6785..7876)
                     /gene="nagA"
                     /locus_tag="PKM49_00800"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HNY81383.1"
                     /translation="MVFNEVLIVDPVIGCAPGRVETHNHLISAVIPQECKPEYLLMPG
                     FVDLHTHAQKGIDTMFAGQEEYTRWAQNNFEQGVTTFLPTTVSASIKNLKNVLRKADR
                     LPLSIDGIHLEGPFINPAKKGAQNAEFIISAKDCDLSEIIKEPVRIVTAAPEVEGFDT
                     LHEECQKKDAVLSIGHTAATYETMKRAYEMGVRRITHFPNALSPLLHREIGATGAGLY
                     LDFDLEMIVDGIHSSPEFVDLVYRVKGSDRILLITDSMAAAGLEDGEYTLGGLAVFVK
                     GGKAVLKDGTLAGSTLLFIEGVRNFYRFTRCSYGDLVKVSSWNALKQLKLEATVGRIH
                     PGYLANFVLLDKSLNLKKTVFQGELVFSA"
     gene            complement(8292..10550)
                     /locus_tag="PKM49_00805"
     CDS             complement(8292..10550)
                     /locus_tag="PKM49_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY81384.1"
                     /translation="MKITTRLLLGFSLILLLLVANFWIFQMVAQKTERDSQAILANIQ
                     KTKALTQENRNSFEFERLILQTVRDFLRVGFVSDKDQRAELGDVYFQNYAKAEAMAKE
                     YGFGKEVEAILDELDDFIQKVMASKDKELSSQRIMDTTKNERIPKQQARLNEILRQID
                     VYTRPDATKIATVEKMLVNADTAFSPKEFKDALSAFTFREVEQLWASKQYVFDTDVVF
                     GWEFLIRSYLSKLMEDSVFLYQFNQKLISEKKLSTYLQKSELSPEERYAFVNLATEWA
                     YGKSFSVYDELFIELTITQKEIDDTNLSITRLQDRLYTQREEGLTIVNEGMATTVTKF
                     EQLVRSLFLKKAQEIDANYATFQKSVRETVDSLADTNRLFVMILIGMLVITVIVIIWI
                     VRGFRKPLKALKEKVRAIAGLDLTVDFSTKAKDEISVAENELKHVVTAFRNTLKGISG
                     ASNSLGDASEELRGLAEESSQGAQKVLEQNKTVVNSIQTVSDSIEEATRIIRTLSDAT
                     KESSQLSLTLSKESVDAAKQTREGAAIVEEIAAMAVKTRDNTVLTSKRVATLTEKTQN
                     VGDILNTISNISEQTNLLALNAAIEAARAGEAGKGFAVVADEIRKLAEESKKSTQDIS
                     KILGEIEGSVVQANEATQLTAGFVEEFSRKTDEIQKQYAQILEKFNKISTITQKTAAA
                     SKDQSDDSGDIAQKMESNARALAKVGEGIRSMMERTRGQTATAENVLKSSEALLQLSG
                     ELQNEVEHFKIG"
     gene            11390..12502
                     /locus_tag="PKM49_00810"
     CDS             11390..12502
                     /locus_tag="PKM49_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011015931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNY81385.1"
                     /translation="MALNYIGSKLSLLDFIENVVGYAIDEKIDVVGDLFAGTGAVGKL
                     FKAKGCKIVSNDIQHYSYVMNKKHIGICAPLSFSGLVSELPGLEAAEDKTSFVLDHLN
                     NSTALYSGFIVENYSEAGTRGKKHTRLYFSDENALLIDTIRQRIETWAVDRKITEDEY
                     YYLLATLLEAADRVANTASVYAAFLKVLKAPATKRLELRQDEIIPSNRTHKIFNDDIR
                     NILNREPIDLLYLDPPYNGRQYDTNYHLLETISRYDNPEITGKTGLRKSKDQKSDFCR
                     KRTVKQAFAQVVLHTQAKYILVSYNNEGLMSLSDVVEILTLRGTPKTYLRQYKRFRAD
                     KSENRNYKAAATDEFLHVVRCDRSFKRYSEEKRVSW"
     gene            complement(12515..15568)
                     /locus_tag="PKM49_00815"
     CDS             complement(12515..15568)
                     /locus_tag="PKM49_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNY81386.1"
                     /translation="MSEVGLNASQKRILETEGSLFVSAGAGTGKTHAIVEKFFALIQD
                     PLKRVDPLSVAAITFTKKAAGEMKERIIRHLEDLSGKPNMATEAERILNAMPFVWIDT
                     IHGFCSRILREFSLEMNLDPSFEVLNEYQKERKIRGIVLRELDQEIGGKKPEEVKLLL
                     NQFRLDGLIDLFIQALSRKRFEVWDIFTPRAGESEPTDEVAKAFGNLFKRILAVYEEE
                     CARDNAVDFEALLLHTLRLLDEHEGVREHLAERFHAIIVDEFQDTNVLQKRILDRFAE
                     CGKTTLIYVGDGKQSIYRFNGAEIGVFNVTREQFDETRYVTLEENYRSHEKLIDFYNR
                     FFPKVFGNQPDRYGIAYEPLTPGKTDKEDRFSAHAGRVRLLPKAKDFAEECFNLCAYI
                     THLIDKEHRQPKDFAVLLRAMTAVDTLTAVLERYGLPYYQVGSTNFYRRPEILSLLSL
                     VRALYNPADEYALVQLLSSYLSPFCEEEILEMRQRDRRFLYTGLKAYVKEIGSTDSQK
                     GLFLERIEGLRAHLPLRSIGELLREAIQDFDYDLYLATLPNGPKAWLNVKKLLEIADG
                     FTGNASTREFLEQMAQENVIKEEEAAIESEHDDVVRVMTIHKAKGLQYPIVILPNLGF
                     NQSPRSHIFLTDWENRLIFFSGDAEGEAGEDPVDQEDNAVRASETAKDYSELKQKEKA
                     REYEENKRLLYVAMTRAKEEIVLSFSERPRNRQNRPAPPKKGFLPLLHTGGLIVPGTE
                     WAPIEESEYAGLLTPAPQDLKTIRAVDQAVEPSGPSTGETVERIPQTAFPLEDRPYQK
                     YLSPSLLTSVEKTDAQATRRILWRHEEKKTDDLLPELFKLSFGAPGYTEEVGTLVHKV
                     LERYGDKDLSGITRETVDRLNRSFGFPDSVVEATEAKIRRVAGHPLFDDLRKAHPCFS
                     EKPIRKLRGRYILSGTIDKLYFLDGKWHILDFKASRANPQYLERYRFQMRFYLFLVKG
                     LVQPEPEKATLFFLDERETMDVVWDAGFEEELDRRIGDYERAFVN"
     gene            complement(15565..18807)
                     /locus_tag="PKM49_00820"
     CDS             complement(15565..18807)
                     /locus_tag="PKM49_00820"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNY81387.1"
                     /translation="MAKIVIVKGIPSSGKTRHILNRVAEAHREDPFSYLVLGPSGSFI
                     RNLRERFLALVGNFFNDSFLLLDQYVVQFLLNRGDGGFYINRSFLAGFVAGKVREEHR
                     LYPLLQRGQGMIDAFLQFYSWLKDHDASALLGEMEQTEDTLLKEFAGLYRIIDTQLRE
                     KRLFTVEDAYYRVLDLIEKKAVQPQEVPYRKLFLDGFFDLTPKTRRFFYTFFCFFEEV
                     YLSAPLNPDGSLMDLSFDAFETEPSHLRTFHRWETISPEGAEDKLSGSLKDTLNLLDK
                     PPSLISASSSASSATPFDILECSNPHQEMIYACNWVKQLIQEKKYVPEDIGIVVRDER
                     KYLPLLKEGFSQNGVPFRFEGDLPLNRSLNVNKLLLPFRAFSGGFSPDSMLALLETGF
                     VELPESLPFPEFETLAMRAKLAYSNAFYNDAPVTLEWRKRDWNERLTRYTKLVERKAR
                     SAGALDDDGEALQQAEEERNRLKYANELIRMLFSKLSFFESKNAPTAKEGYILYFQKL
                     SDDYSKRGLLSDTQEEQNATRVFLNEVLPELNRFLYTIDPSEDGSKPVSQRQYWKYLK
                     LFVDLSGYRQSVRIDNRVYLSNLEDARFREKRVKVFVGMTDQNYPLLASGSVFLRAQW
                     PKEQTGGTGAAAPSSSSMRDTIVKKERRDFMNAVRSTEDFVLFTYPKADISGNTYLPS
                     LFLKPFFRWKERMKTAGKPGDSLLHPDVGAPSAYSLRQFAIETIATERTPSQEVRATL
                     KRARIDLERVRPTQPERAPTPIDQSRYEQTLSSLFGKTYSASKHTTLLECPRKFLYRY
                     MLKLYRPLRVFYGFDYLTEGVIFHNTLKRVFSDEAIRNRMVNPEAFRMDVERILKSEI
                     EPRMYAPSSLLERAENAYFSNVLGQFARSYPERLESLTGKIRKRVPELEALVPTRFEV
                     PFKENAATPFHHKKDASPIYLIGKIDRVDTLPDDSAVIIDYKRTIAPAGVEQLLLYAW
                     ALETLEKQKVSAVSLLQIVSKPGAPNKDGIKALVSNSESPNLFDDPTNRRVHFKDRDQ
                     VVKTAREAIDGVLRGDFSRKTARCWQCEYVGICRFRSDKDEDSGEEEVSQ"
     gene            18977..21499
                     /locus_tag="PKM49_00825"
     CDS             18977..21499
                     /locus_tag="PKM49_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY81388.1"
                     /translation="MSREKRNILFVVLFMCALLTLFAERLPWDDQIIYFVMIDRFWNG
                     DPLNDVQTPDGVEAGVENSKYNGGDLKGLIERLDYIKELGATAIWITPPVANQWWDGS
                     VQYGGYHGYWARNFKAIDEHFGDLELYRDFIQAAHEKNIRVIQDIVVNHVGNYLIYDP
                     VTLMGSVNEQSIPTKKPTQYPFDQNDYYDPAQSALNIYHWPSEIENPDRFNTALSDLD
                     DLNTENPIVIEALKDSYGFWIDQGIDGFRIDTSIYVEDAFWPLFLEDPDGMYARASKN
                     DKPDFLVYGEAWVTPQPYEDTGERVLSNYYDIGYNSMLDFPLMTELRRVFKEGKPTQM
                     LEYRLQQRENYFSGKRLITFVDNHDMERFLKGVSMRDLKQALLVIMTIPGIPSIYYGT
                     EQGFLETRATMFKEGFESGGVDHFRTDHELYQYLDKIIQLRKETPAFRYGRVTVVHSD
                     PIGPGAFIYRVQDSNSTYFIFLNTSSQSRYATQMEMGVAEGTVLEPIFTEGIIFKNVV
                     VGQNGLLNYLIAGKGLGVFRATDETQEVKGFDIDVAMTSIRENEMIREDFVLRGTSRN
                     AARVRLYVDGSEREYATVVPDAEGHWDVPVRISEFISGAHRLFVRAYGKKATESVYSQ
                     TMTVFFDIPTVHLVEVTDPEGDDKGPTGDYGYPKDLTFEHQQDVLHVKLSQVGRMLRM
                     DITMKNLTDVWSPTNLFDHVTFQIFFDRPDRTGALALPFQNATMPEGMDWDYMIYATG
                     WSMALYESDEKSGPAYYGTPITPAPTVQVDKQLKTITLLVPLEVLKTDRLSGWKIYLT
                     TYDYDGIEAVLRPISPGGGAWAFTGPSALAPKIMDFVLIDIP"
     gene            complement(21547..22644)
                     /locus_tag="PKM49_00830"
     CDS             complement(21547..22644)
                     /locus_tag="PKM49_00830"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNY81389.1"
                     /translation="MENFRKMLTIVILILLQFSSQVGFAQPTLAERIDKRCEEEHTWM
                     GFWGVVLVANGEDILFEKGYGFADCDHTRPIHPELKFRIASITKQITAAAILKLSEEN
                     RLSLQDPLSLYLPAFSTLPQITLYQLLTHTSGIVTLLNTSPDLNDRIQRFPGDTTLDK
                     TLAYLRAQPLRFEPGEGFYYSNGGYAILSAVIESVTGLTYPDYLKETFFIPLGMIDTE
                     YDPNVYDDHWAIPCHRLSVQPDRVEPAPWVNRDIPYGAGGLCSTARDLLKWSVALSEG
                     KVLKPETLRLMETPQSEVYGNGLGVFIEYPPFNGVRYRLVFHDGATAGSSTHLSRYVD
                     KGLTIVVLSNLDDYDFTMLAYQLARIVFSEE"
     gene            complement(22728..23753)
                     /locus_tag="PKM49_00835"
     CDS             complement(22728..23753)
                     /locus_tag="PKM49_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008681985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY81390.1"
                     /translation="MKNKTPFNPFRYGIWVLLLFSFLTGFAFSGTFPGPSLSGVVVRE
                     ALRIMVWSLKSRGAGIIDTGTASAQSHKQWKAPWGFSHMKYIVQGVPVELLVKKRGTP
                     ERVILLLHGGAYLMGLVDIYRDLGVRYSTISGGASVALVDYRIAPEHTFPAALEDAEA
                     VWNWLLEQGYREENIIVVGDSAGGNLSLALALKLRDEKRKMPKAIVCMSPWTDMTASG
                     DSYRDNLYKDPIFGLFPWEKPTGNGQWIGNLYIGGHPLDDPYLSPAFGDYRGFPPMLI
                     QVGALEIIESDSLIVYERAKAAGVDVTLTRYEGMFHVFQLFGDFVPEVREAWEEVTDF
                     LRWQFRR"
     gene            complement(24151..>24815)
                     /locus_tag="PKM49_00840"
     CDS             complement(24151..>24815)
                     /locus_tag="PKM49_00840"
                     /inference="COORDINATES: protein motif:HMM:NF019623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HNY81391.1"
                     /translation="IDELMEYPISESLFSAYEIEAAPAASFLTQSGYLTFKGYREKRG
                     YELDFPNQEVKDAFSQLLLLHRYGLEPQTNDAIRNGILNGLDKRDFGIIFEQMRITFA
                     SIPYTLYHKREEQKGNHPERLERFYHVVLLTLFWGCGIEAKAEEATHLGRSDLVLAYG
                     EDVYIMELKKAPAEKALQQIREKGYGEKYRGKNLYYVGIEIDTEQRNLKGYRIEQSAP
                     AV"
BASE COUNT         5673 a         6428 c         6599 g         6115 t
ORIGIN      
        1 tccgaagatc aaaaaacaga gttctcgcag aggccacgga gaacacagag gaaaaactgg
       61 caagggacag ggctctcccg gaaaaagacg atacgtgcca acttaaaagc cgacgtttcc
      121 aggcgttcct aggcgtgtgg cgttcccctg gggtttctct gcgccctctg tgtactctgt
      181 gcgaaacttc ttttggtttt gaaaagactt gccgactaaa gttcgacgtt accaggcgtt
      241 cccagggtgt tcccggcgag aatcccccct ctgtcttctc cgtgcgctcc gtgtgaactc
      301 ccgttttgtt ttttagacat accatcttaa aagttggcgt tcccaggcat tctcagtgac
      361 aatccaagaa agatgttccc agagatactc ccagaaaggc cgttcgcctc tctctcggaa
      421 aaactgtact ctcttgactt ctatatcgga agctgatata atatatcgga atacgatata
      481 acgtcaggcg taatagagga ggcaggggat gtgtcagaaa atatcgggcg gggcgcgttg
      541 acggaagcgg tgttctacat cctgttgtct ctaatgacgc ctatgcacgg atacggcatt
      601 atgcaaaacg tgagtcaact cagcaaggga cgcctggcat taggggcggg taccctatac
      661 ggcgcgatca acacgctttt agaaaaagga tggatacggt ctgttccggg cgaagcggac
      721 tctcgaaaga aggagtacat catatcggac ctaggaaaaa acgctctcga gaatgagctc
      781 gtccggttgc aagagttgtt gcagaacggg gcagcgataa tgggagggcg aatttcgtga
      841 aaagagcaat ctggagacta tatcttaact tcgaaaaaga agaaaagtgg ctgaatgaaa
      901 tggccacggc aggctggatg ctcaccgatt attcctggtg tcggtacgtg ttcgaagagg
      961 gacgagaggg cgaatacgtc taccgaatcg agttattaaa aaacttgccc acgtacccat
     1021 tgtctcaaaa atacctccgt tttctcgaag aggctggtat tgaatatgtg gcctactacg
     1081 cgagatgggt gtatttaaga aaaaaaaccg ccgacggtcc gttcgacctc tataccgatt
     1141 tggagtcgaa gatcgcgcac taccgacgcg tcttgcgcta ctggcacgtg ttcgcaggga
     1201 tcgaaatgac gatcggcctt tcctcttgcg cgcccgcact gaacgcttgg tggcgcggcg
     1261 gcgttctatc gtatagccta tggatgagtg tcatcccgtt cgcgtttggg attctgttcc
     1321 tattcattgc tgcgcccata aaaaggcagt tgaagcgttg gaagaaagag ctgcagattc
     1381 gagagtctta ggaacgcgcg atcgtgttat aatggcggga gtattcgtgc agacatacgg
     1441 acgaaggagg ggtctacacg tatcgacgct tcgaagaacg aggaaacaac gccttaccgg
     1501 ctttggaaac tgtattgtac gacgggtggc ttctccgttt cgcgtctggg tatacccgtc
     1561 gcgcgaactg tgtgcatccg ctttacccat caaacgtaaa actgatcgag aagatcccct
     1621 actgcgaagc cctctttcga gaaaaggaca tcctttgccg ttttaaactg aacgacgacc
     1681 ccgatttgac cgaactggac accgcgctgg cagcgcaggg ttacacgctc gacgcgcccg
     1741 cgctcttaat gcatcgcccg aagggttgtg acatcccgcg agagcaggga aatatcgagg
     1801 tttccgactc ttttaacccg gaatggatgc ggtttttcgt ttccgcgaaa gggcttgatg
     1861 accgtgatgc gcggaccttt gaatatatgc tccgtcacat cttgcccgaa cgacggttca
     1921 ttttgaagac cgagggcgga ctgactgtcg ggtgcgttct ggccgtatat gagcggggat
     1981 tcaccggtct attcgatctt tacgttgatg aagcgcatcg tgggaaaggg cttggattgt
     2041 ctctagtaca gcaggcgctt gtcgaaggcg ctagaatcaa ctgccccgaa gcgtatttgc
     2101 aggtcgacga gacgaacgag cgtgcgatcc gtctctatcg cggcatggga ttcgaagtgg
     2161 cgtatcggta ttggtatcgt tactcggcgg ccttaaaatt gtagatcggg cggatgatcc
     2221 gacgaacgtc aacggtttgg ccgagcacgg gtaaaatctc ttccatcgcc ttgtagacca
     2281 tcggggactc gtcgatcgtc gttcgatcca cgcaggtgga gaaaacccct ttcatctgat
     2341 cccgaaattc atcaatcgac aggattcttt tcgcctctcg ccggctcatc aagcgtcccg
     2401 cgccgtgggg agccgaccaa ttccactccg ggttgccctt tccagttccc aaaatgcacc
     2461 cgtccctcat attcaaaggg atgatcacgg gttgtcccac atgcgccgcg atggccccct
     2521 tgcgaaggat atttttctca tcgatgtagt tgtgaaccga ttcaaagctg gccagttctt
     2581 tgagccccat cttctgcagg atcctttcgg cgatccgttc ccggtttcgt ttcgcgtagg
     2641 tttggatcag ccggatgtcg tgcagatagt tattcctgtc cgatcccgtc agataggcta
     2701 agtctttagg tgtgcctttt gcgcgtgagc ggcgagcttc gccgttttgt tgcatcttgc
     2761 ttgccgcttt cgcctgatac agctgggcca cggtcaaccc gaaatgacgg gacccggtgt
     2821 gaacggtcag atacgagcgc ccctcttcat ccacatcgac ctcgatgaaa tgattcccgc
     2881 ctcctaacgt accgagcgac cgttcgagcc agcccatctt ctcgcggctc ttgatttgat
     2941 ccttgatgat caggtcgttg aacaaagaat aaaagaagtc gcgatcttcc ggaaatgtgt
     3001 gacgatggac gttctgtcct gaaggaacgg actcgcggat atccttatcc aactgttgca
     3061 aattgattgg agttactccc aactccgata cgcggacgcc gcatccgata tcgacgccga
     3121 caaggttcgg gcaaacggcg tcggaacggt tcaggtccat cgtcaagcct atcacgcaac
     3181 ccgctccgct gtggacatcc ggcatcactc gaatatgcgt cccttcggaa aaaggctgat
     3241 tgcaaagcga aacgatctgc gtgatggcgt tctcgtccac agtccgcgcg tagaccaaag
     3301 ccgtggcaaa ctggcctttc aatctgatcg tagggatact gtgatcatcg tttttctctt
     3361 tcattttcca cctcctcact aagcgcgtcg tgggcgtttc tcacaacatt ataacactca
     3421 aaaccgaaag acgcggcaca tgacgccttg cgccttcgta agcctttatg agagaaaacg
     3481 tcgtatggcg tgtgtgatta gccacacagc acgctcggta tgaaagcgcg ggaaatggga
     3541 acgccgaact ttagttcggc acgttttttg cttttctgta agctctcggg aacccttaaa
     3601 cgcttacgcc gcaatgcccg cgcgaggtta tcgcggggta accagcgggc tgacttcgga
     3661 agcccacgaa gtctcgcgat actaacgaaa tccagcgatg cgctctctcc tgtctttcgt
     3721 ttttaagaca cgccaacaaa aaaagttggc gttcccagag aggcaggccg actgaaagtc
     3781 ggtcccacga gctttcttcg gtcttttctc tgtgttctct gcgccttctg tgcgaaccct
     3841 gtttttcaag acaagtacaa aagtatatcg cgccgtcgca tgaagaaggg gcgcttacgc
     3901 cgcgatcacc gtgtggcatt atcgcggagt aagttgtttt tacgaccccg cgatctgcgc
     3961 ttacgccgcg atgactatgt gggctatacg cgattgattc tggtcttgtt tcttgattac
     4021 gcctcatagc ttgacccaca cgtatagaag attcgcggcc gcgatttgct ataatcagaa
     4081 acggggcgct tagggttagg ctcgcgaatc cgaaggccgt agggttcatt cattgccaga
     4141 gcgagccaaa tctcactttc attctctttt cgaaccgaag gtcggaaatg actcgtttca
     4201 ttaatgttcg cctaaggggt taggagctta ttgtgagtcg ggtagactcg actcgaacgc
     4261 ataaggggtg aatgatgatt aaaccaaaga aactcaagcg gggcgacacg atcgccgttc
     4321 tgtccccctc ttccggagcg ccacaaaaat tcccacatat ctttgaacac ggacttagca
     4381 tactcgagag agaattcggt tttcgtgtca aggagtatcc gacaacccga atgtcgtcga
     4441 aagcgctata tgagaatccg aaagcgcgtg cggaggatat gaaccgggcg ttcctcgacc
     4501 cggaggtgaa aggcatcatc tgttccatcg gcggggacga ttccgttcgc attctccggt
     4561 atttggatac cgaatcgatc ctcaagaatc gcaaactcct gatgggatac tcggatagca
     4621 ccaccttcct cgcctatcta aacaagttag gattggtgac ctattacggg ccctcgatca
     4681 tgtccgggtt ttcgtatttg aattgttttc ctgaagcgct ggaggaatac aagagggtgt
     4741 tcttcgaaaa cgccccgtat gaaatccaac ccttctcggt ttgggccgac gcgtatcgac
     4801 gttgggaata ccccgagaat accgggaaag tcgagagaat gagagaggac catacccatc
     4861 acgtctggat caacaagggc gcagtctcga ctggttgtct gtggggcggt tgcctggagg
     4921 tcctggatat gatcaacggc accttcgttt ggccggagaa agcgttttgg gagggtcggg
     4981 ttctgatgat cgaaacctcc gaagacaaac ccagcccaag acaggtcggg taccgtttga
     5041 ggaattatgg ggcgcagggg atcctctcct ccatccaggg gctcctcatc ggaaagccca
     5101 aaggttacac ggtagaggaa atgcgggcgc ttaatgaaga agtgaaaaag gtcgtcgtcg
     5161 gtgaatacgg atgtgatggt ttgaatatcg tcggcaacat cggcttcgga cataccgatc
     5221 cccaccatat cctacccatg ggcgtaccct tgacgctcga tccggagaac gaaaccctca
     5281 ccttcgtcga gcccctcttc tcttcgtgag ttcggttttc tgggagcgcc tttagggcgc
     5341 cgcactttag tgtggcatgg gcttcattgt agctcctggg aacgccacaa tttagtgtgg
     5401 cgtaggttgt gacttatttc atttcaaaaa ctggaaaacc tgccaactta aaagttggcg
     5461 ttcccaggtg ttccaaggcg ttaccagcgt gaatctcccc tctgtgttct ctgcggcctc
     5521 cgtgtgaacc cctgtttttt taagacatgc cgaactgacg ttcggcgttc ccggcggcgc
     5581 tcacagagca aaacaagccg ataataaaaa atggtgtgta aggctaaaaa agtggtataa
     5641 taccgcaatt tttataggga gcgtgaaaat cgtggggaac tgaaattgcc taaaggctac
     5701 aaaagcaagt tgaacctgcg cgacacagaa agtgcgatta aaaaattcaa agacttcttc
     5761 gaaagagcct tatcttatga acttcacttg atgcgcgtct ccgcgccttt gtttgtaaaa
     5821 cccgaatcag gattgaatga caatttgaac ggggtagagc aacccgtggg tttcgtcatt
     5881 cctgacgcgg gtcactgcca agcggagatc gtccaatcct tggcgaaatg gaagcgtatg
     5941 gcgttgaagc ggtatggatt caagatcggg gaaggccttt acaccgatat gaacgccatc
     6001 cgtaaagatg aggcgctgga caatctccac tcgatctatg tggatcaatg ggattgggag
     6061 aagattatcc cgaagaagga tcgaaccctc gaggtactga aagagaccgt gaaaagtatc
     6121 tatcgggttt ttaagaacac cgaacgcttt atcgcttttg agtacaaggt tttggaagaa
     6181 agccaaagcc ttccggacga catcgccttc atcacgacgc aagagctcga agacctattt
     6241 cccgacctca cacccaaaga gcgcgagcac gagattgcca aggaaaagaa ggcgctcttc
     6301 ctcctgaaga tcggtggccc gctcaaatct gggaaacccc acgatgggag ggcgccggat
     6361 tacgacgatt gggagttgaa cggggatatc ctgttttggt atcccctttt agagaccgct
     6421 ttcgaagtct cttctatggg catccgtgtg gatgaagaag gcttggtcaa gcagttaaag
     6481 gtcgccgggt gcgagcagcg tttatccttg ccctttcatc gagcactgct ggatagacag
     6541 ttgccgtata cgatcggcgg agggatcggg caatccagga tttgtatgtt ctttttgcaa
     6601 aaagcgcaca tcggagaggt ccaagcctct atctggccgg aagagacaca tcaggtttgc
     6661 tcggccagcg gaatctgctt attatgatcc cgattccttt ctcccgcgcg aggtaggttt
     6721 ctccaaacat caaaataacg aacgaaaaac ggttttttgg gtgagcaaac tacgtggcgg
     6781 cggatcaagc agaaaacacg agttctccct gaaagaccgt ttttttgagg ttcaagcttt
     6841 tatcaagcaa cacgaaattg gccagatatc ccggatggat tcgcccgacg gtcgcttcga
     6901 gcttcagctg cttcagcgcg ttccacgagg ataccttgac gaggtctccg taagaacagc
     6961 gggtgaaacg atagaagttt cgtacccctt ctatgaacag cagcgtgctg cccgcgagcg
     7021 ttccgtcttt caagacggcc ttaccgcctt tgacaaagac ggccaatcct ccgagtgtgt
     7081 actccccgtc ctctagcccg gcagccgcca tcgagtcggt aattaatagg atgcggtccg
     7141 accctttgac gcggtatacc aaatcgacga attccgggga ggagtgtatg ccgtcgacga
     7201 tcatctctag gtcgaagtcc aagtacaacc ccgcgccggt tgcgccaatt tcacgatgga
     7261 gcaaagggct aagcgcgttt gggaaatgtg tgattcttcg cacccccatc tcgtaggcgc
     7321 gtttcatagt ctcgtaggtg gcagccgtat ggcctatcga aagcacggca tcttttttct
     7381 ggcattcctc gtgtagggtg tcaaacccct cgacttccgg cgcggcggtc acaattctaa
     7441 cgggttcttt gatgatctcg gataagtcgc aatcctttgc ggagataatg aattcggcat
     7501 tttgcgcgcc ttttttcgcc ggattgatga acggcccttc caaatggatg ccgtcgatcg
     7561 ataaagggag cctgtccgcc ttcctcaaga cgtttttcaa gttcttgata gaggcggaaa
     7621 cggtcgtggg caaaaaggtg gtcacccctt gttcgaaatt gttctgcgcc catcgggtgt
     7681 attcttcctg gcctgcgaac atcgtatcga tccccttttg cgcgtgcgtg tggaggtcca
     7741 cgaaccccgg cattaagaga tactccggtt tgcactcctg tggaataacc gcgctgatca
     7801 ggtgattgtg cgtttctacg cgtcccgggg cgcatccgat aaccggatca acgatcaata
     7861 cctcgttaaa aaccatagaa ccaccgcctt tgatggaata tgaagcgggt ttccgcgaca
     7921 gtataactat tctccatatt atgaaactct cttatggact gtatcatacc atttttcgca
     7981 tgatatgtca attgacgtga atcagtaaac tattaagcac tttatttgat aataagtttt
     8041 cttgattcac tgaattctcg gtataagtga acgataggtg tctattgatc tctaaattcc
     8101 ttcgagtctt gtcctcagcg atttttccag gaggatgaaa tgaatgcggt agcggtcggg
     8161 ggttgttttt tgcttttaag gctgagagat gtcgagtaaa ggacgagcga gggaaacgaa
     8221 tcggagcggc cggttaaaaa agagcgcggg tagatccgcg ctcttttagg cttttcgata
     8281 acgagcgtcg attatccaat tttgaagtgt tccacttcgt tctgcaattc tccggagagt
     8341 tgtaggagag cttcggacga tttcaggacg ttttccgcag tcgcggtttg accccgtgtt
     8401 cgctccatca tcgaacggat gccctccccc actttcgcca gcgcgcgtgc gttgctttcc
     8461 attttttgtg cgatatctcc ggagtcgtcg ctctggtctt ttgaagccgc cgcggttttt
     8521 tgcgtgatgg ttgaaatttt gttgaatttc tccaagattt gggcgtactg tttctgtatc
     8581 tcgtcggttt ttctactgaa ctcttccaca aatccggctg tgagctgagt ggcttcgttc
     8641 gcttgcacga cgctgccctc gatctcccca agtatcttgg atatgtcttg cgtcgatttt
     8701 ttgctttctt cggcgagttt tctgatttcg tcggccacca cggcgaaccc ttttccggcc
     8761 tctcccgctc gggcggcctc tatcgccgcg ttaagcgcca acaggttcgt ttgttcggag
     8821 atattggaaa tggtgttcaa aatgtcgcca acgttttggg ttttctccgt caacgtcgcc
     8881 acacgtttgg acgttaaaac ggtgttatcg cgcgttttca ctgccatcgc cgcgatctct
     8941 tccacgatgg ctgccccttc tcgcgtttgt ttcgcggcgt cgacggactc tttcgacagc
     9001 gtcagggaga gctgcgaaga ttcttttgtc gcgtcggaca aggttcggat aatacgcgtg
     9061 gcctcctcga tcgagtcgct caccgtttgg atagagttga cgacggtctt attctgctcc
     9121 aacaccttct gagctccttg tgaggactct tcggctaacc ctcgaagttc ctccgaggcg
     9181 tcaccgaggc tgttggacgc cccggatatg cctttcaatg tatttcgaaa tgcggtaacg
     9241 acgtgtttga gttcgttttc tgccacgctg atctcatctt tggccttcgt cgaaaaatcg
     9301 acggtcaggt cgagccccgc tatcgccctc accttttctt tcaatgcttt caacggtttc
     9361 cggaacccac gcacgatcca gatgatcacg atgactgtaa tgacgagcat gcctatcaga
     9421 atcatcacga acaggcgatt ggtgtcggcg agggagtcga ctgtctcgcg tacacttttc
     9481 tggaaggtcg cgtaattggc gtcgatttcc tgggctttct ttaggaagag cgatctcacc
     9541 aactgctcga atttcgtgac cgttgtcgcc attccctcgt ttacaatggt caatccttct
     9601 tccctctggg tgtaaagacg atcctgcagg cgtgtgatac ttaggttcgt atcatcgatc
     9661 tctttctgtg tgatcgttag ctcgatgaac aactcgtcgt agacggagaa gcttttccca
     9721 tacgcccatt cggtcgcgag gttaacgaaa gcatatcttt cttcgggaga caactcggat
     9781 ttttgcaaat acgtcgatag ttttttctcc gatatcagct tctggttaaa ttggtagaga
     9841 aataccgaat cttccatcaa tttcgacaaa tagcttcgaa tcaagaattc ccatccaaac
     9901 acgacgtccg tatcgaacac gtattgtttc gaggcccaca gctgttcaac ctctcgaaag
     9961 gtgaaagcag atagggcatc cttaaactct ttgggggaaa aggcggtgtc cgcgttgacc
    10021 agcatttttt cgaccgtggc tatcttcgtc gcatctggac gagtatagac gtctatctga
    10081 cgcaggattt cgttgagccg cgcttgttgt ttaggaatcc gctcgttttt cgtggtgtcc
    10141 atgatgcgct gtgaagaaag ctctttgtct ttggatgcca taactttctg aatgaaatcg
    10201 tccagttcat cgaggatcgc ttcaacctct tttccaaaac cgtattcttt cgccattgct
    10261 tccgctttcg cgtagttttg gaaatagacg tcacccaatt ccgctctttg gtctttatcc
    10321 gaaacgaaac ccaccctcaa aaaatcacgg actgtctgca atatcaaacg ttcgaattca
    10381 aaggagtttc ggttttcttg agtgagcgcc ttcgtctttt gaatgttagc gagaatcgct
    10441 tggctgtccc tctccgtttt ttgtgccacc atttggaaaa tccaaaagtt cgctaccaac
    10501 agtaacagaa tcagagaaaa ccccaaaagc aatcgagtcg ttattttcat attctatgac
    10561 ctcccataga gagaattgca tattcgtgct ctattataac acccgccgct gttctaggct
    10621 atacatttcg gtatgcgttg tgtaaaggta tcgcgttgac ttattcacta agaaacaagt
    10681 cctcatttgg cttgtctgag tcgcaggttt tgcaacgtcg agtgttctat cacgctcgaa
    10741 aagatttaag acttccgtct ccggttattt tcgactttct gtgtgggtca agctatgggg
    10801 tgtaatcaag aaacaatgtc agaatcgtcc gtacgatttt tattcttgta gtcatcgcgg
    10861 cgtaagcgct tcttcttttc gcgacggcgc gatacgcttt tgtacctgtc ttgaaaaaca
    10921 gggttcgcac agagggcaca gagaacacag agaaaagagt gaagataact cgtggcgccg
    10981 acttccagtc ggcctgtcgc tcggggaaga ccattggaac gccaaacttt agtttggcat
    11041 ggtttgtgaa aattgaaatg cagcagagcg tatcgcgaag gtatgaaagt atcgcgagac
    11101 tccgtgggct tccgaagtca gcccgctggt taccccgcga tagctccaca cggtcatcgc
    11161 ggcgtagaag gggaagggca gatcgcgggg tcgtaaaaat cacttacccc gcgataacca
    11221 tcttagttac gtcaccccat agcttgactc gcacttcaga tattatgaac atcgcgggta
    11281 tggtatactt gatacaggca gccttagaag agcctcgcga cgtttcttag ggagcagcga
    11341 ggaaaaacgc gtcgggctgc gtcaggctca aaccgcaaag ggtgagaaga tggcgctcaa
    11401 ttacattgga agcaaactgt cccttttgga tttcatcgaa aacgtggttg gttatgcgat
    11461 agacgagaag atcgacgtcg ttggggactt gttcgcagga accggcgcag tgggtaagct
    11521 cttcaaagcg aagggttgta agatcgtatc gaacgatatc caacactaca gctatgtgat
    11581 gaacaaaaag catatcggca tatgcgcgcc gctgagcttc agcgggttgg tttcggaatt
    11641 gccggggtta gaagccgcag aggataagac aagcttcgtc cttgaccatt taaacaacag
    11701 tacggcgctg tactctggat tcatcgtcga gaactacagc gaagcgggga cccgcggaaa
    11761 gaagcacact cggctgtatt tttcggatga aaacgccctg ttgatcgaca cgatccggca
    11821 gcggatcgaa acctgggcgg tcgaccggaa gataaccgaa gacgaatatt attatctact
    11881 agcaacgctc ctcgaagccg cggatagggt ggcaaacacc gcctccgtgt acgccgcgtt
    11941 cctgaaggtc ttgaaagcgc ccgccacaaa aaggttggag ctccgacaag acgagatcat
    12001 ccccagcaat agaacccaca agatctttaa cgacgacatc cgtaacatcc tcaaccgaga
    12061 accgatcgac ctcctgtacc tggatccgcc atacaacggg cggcagtacg atacgaatta
    12121 tcatctgctg gagacgatct cacgatatga caacccggag atcaccggta aaacggggct
    12181 gagaaaaagc aaggatcaaa agtccgattt ctgtcgaaaa cggacggtga aacaggcctt
    12241 cgcgcaagtg gtgctacata ctcaagcgaa atacatcctg gtgagctaca acaacgaggg
    12301 tctgatgagt ctatcagacg ttgtagagat cttgactctt cgtggcacgc ccaagaccta
    12361 cctccgccaa tacaaacggt ttcgggcaga taaaagcgag aaccggaact ataaagccgc
    12421 ggctaccgac gaattcctac acgtcgtcag gtgcgataga agtttcaaac gctattccga
    12481 ggaaaaaagg gtatcttggt aaacctttct ccattcaatt tacgaaagcc ctctcgtaat
    12541 cgccaatcct tcggtcgagc tcttcctcga agcccgcatc ccagaccaca tccatcgttt
    12601 ctcgctcgtc gaggaaaaac agggtggcct tttccggctc cggttgtacc aaccctttga
    12661 ccaaaaaaag atagaaacgc atctgaaacc gatacctctc gaggtattgc ggatttgcac
    12721 gcgaggcttt aaagtcgaga atatgccatt ttccgtccag aaagtacagc ttgtcgatcg
    12781 tgccggataa aatgtaacga ccccgcagct ttcggatggg tttctcgctg aagcacgggt
    12841 gcgccttccg aaggtcatcg aacaacggat ggccggcgac acgacgtatc ttggcttccg
    12901 tcgcttcaac gaccgaatct ggaaagccaa acgaccggtt cagtcggtcc accgtctccc
    12961 tcgttatccc cgaaaggtct ttgtctccat accgttccaa gaccttatgg acgagcgtcc
    13021 cgacttcttc cgtatacccc ggggccccaa acgagagttt gaagagctcg ggtaagaggt
    13081 catcggtctt tttttcctcg tgcctccaca ggatacggcg cgttgcttgc gcgtcggttt
    13141 tctcgacaga tgtgagcaat gaggggctga ggtacttctg gtaaggccga tcttccaagg
    13201 gaaaagcggt ctgtgggata cgctcgaccg tttctcccgt cgaggggccc gaaggttcca
    13261 ccgcctgatc taccgctcga atcgttttga gatcctgcgg cgctggcgtg aggagtccag
    13321 cgtattctga ttcttctatc ggcgcccatt ccgtcccggg cacgattaac cctccggtat
    13381 gcagcaaggg caggaatcct tttttcggcg gggcaggtcg attttgccta tttctcggcc
    13441 tttcggagaa gctgagcacg atctcttctt tggctcgcgt catcgcaacg tatagaaggc
    13501 gtttgttttc ttcgtattct cgggcttttt ccttctgttt caactcggaa tagtccttcg
    13561 cggtctcgga agcacgaacg gcgttgtcct cttgatccac cgggtcttcc cccgcttccc
    13621 cttcggcgtc tccggagaaa aagatcagcc ggttttccca atccgtgaga aagatgtggg
    13681 atcgagggct ctgattgaat ccgaggttgg ggagtatcac gatcgggtat tgcagcccct
    13741 tggccttgtg gatcgtcatc acccgcacca catcgtcgtg ttccgattcg atggcggcct
    13801 cttcttcttt gatgacgttt tcttgcgcca tctgctctaa aaactctcgg gtcgacgcat
    13861 ttccggtgaa tccgtcggct atttccaaaa gctttttcac gtttagccac gccttggggc
    13921 cgttgggcaa cgtggccaga tacaggtcat aatcgaagtc ctggatcgct tctcgcaaga
    13981 gttctccgat agaccgcaaa ggcaggtgtg cgcgcaatcc ttcgattctt tccaggaaca
    14041 aacctttttg cgagtcggtg gagccgattt ctttcacgta tgcctttaaa ccggtataaa
    14101 gaaaacgccg atcgcgttgg cgcatttcga ggatctcctc ttcgcagaag ggcgagagat
    14161 aactcgacag gagttgtacg agggcgtatt cgtccgcggg gttatagagc gctcggacga
    14221 gcgagaggag cgagagaatc tccggacgcc ggtaaaagtt cgtcgagccc acctgatagt
    14281 aaggcaggcc gtacctttcc agcaccgcgg tcaaggtgtc gacggcggtc atcgcgcgca
    14341 acagcaccgc gaaatctttg ggttgccgat gctccttatc tatcagatgc gtgatatagg
    14401 cgcagaggtt gaagcactcc tcggcgaagt ctttggcttt agggaggagt ctcacccgac
    14461 cggcgtgggc ggagaatcga tcctctttgt ccgtctttcc aggcgtcagc ggctcgtatg
    14521 cgatcccata acgatccggt tgattgccga agaccttcgg gaaaaaccgg ttgtagaagt
    14581 cgataagctt ctcgtgcgag cggtagttct cttccaatgt cacataccgc gtttcgtcga
    14641 attgctcgcg ggtgacgttg aagacgccga tctccgcgcc gttgaatcga tagatagatt
    14701 gcttgccgtc tccgacgtag atcaacgtgg tctttccaca ctctgcgaag cggtccagga
    14761 tgcgtttctg gagaacgttc gtatcctgga actcgtccac gatgatggcg tggaagcgct
    14821 cggccaggtg ttctcggacg ccttcgtgct catctaacag gcggagggtg tggagcaaga
    14881 gggcttcgaa atccaccgcg ttgtcgcgcg cgcactcttc ctcgtagacg gccaggattc
    14941 ttttaaagag gtttccaaac gcctttgcca cctcgtctgt cggttcgctt tctcccgctc
    15001 gaggggtaaa gatgtcccac acctcgaatc gctttcgact gagcgcttgg atgaaaaggt
    15061 cgatcaatcc gtccaggcga aactggttca gcaggagctt gacctcctcc ggtttctttc
    15121 ctccgatttc ttgatcgagt tcgcgtaaga cgattcctcg gattttgcgt tctttctgat
    15181 actcgttcag tacttcgaaa gaaggatcca ggttcatttc gagcgaaaac tcccgcagga
    15241 ttcgggagca aaacccgtgt atcgtgtcga tccagacgaa tggcatcgcg ttgagaatcc
    15301 gttcggcctc cgtcgccata tttggcttgc ccgaaaggtc ttctaggtgg cggatgatcc
    15361 gctctttcat ctcgcccgct gcttttttcg tgaaggtgat tgcggcgacg gagagggggt
    15421 ccactcgttt caggggatcc tgaatcagcg cgaagaactt ctccacgatc gcgtgggttt
    15481 ttccggtacc ggcgcccgcg ctgacaaaaa ggctcccttc ggtttctagt atcctttttt
    15541 gactcgcgtt aaggcctacc tcgctcactg gcttacctcc tcttcccctg aatcctcgtc
    15601 tttatccgag cggaaccggc aaatgccgac gtattcgcac tgccagcatc gagcggtctt
    15661 tctagagaaa tccccgcgta acaccccatc aatcgcttct cttgccgtct tcaccacctg
    15721 gtcgcggtct ttgaaatgga cgcggcggtt tgtggggtcg tcaaataggt tgggcgattc
    15781 gctattcgag accagggcct ttattccatc cttgtttggc gcccctggct tcgatacgat
    15841 ctgcaacaag gaaaccgcgg aaaccttttg tttctcgagg gtctccaatg cccaagcgta
    15901 gaggagcagc tgttcgactc ccgcgggtgc gatcgtgcgt ttgtaatcga tgatcacggc
    15961 gctatcgtct gggagggtgt ccacacggtc gatcttccca atcaggtaga tcggtgatgc
    16021 gtctttttta tgatgaaacg gggtggcggc gttttcctta aacgggacct cgaaccgggt
    16081 ggggacgagc gcttccaatt caggaacacg ttttcgaatt ttgcccgtca gcgactcgag
    16141 ccgctcgggg taacttcggg caaactgacc caacacgttc gagaaatagg cgttttctgc
    16201 gcgttccaac agggatgagg gcgcgtacat tctcggctcg atctcgcttt tcagtattct
    16261 ttccacatcc attctaaagg cttcgggatt gaccattcga tttcgaatcg cttcgtcgga
    16321 aaacaccctc ttcaacgtgt tgtggaagat cacgccctcc gtcaggtaat cgaacccgta
    16381 gaagactcgt agagggcgat agagctttag catataccga taaaggaatt ttcgcggaca
    16441 ctcgagtagc gtcgtgtgtt tagacgcgct ataggtcttt ccgaagagcg acgaaagcgt
    16501 ttgctcgtag cggctttgat ctattggcgt gggcgcgcgt tcgggttggg ttgggcgcac
    16561 tctttccaaa tcgatcctcg cgcgtttcaa ggtcgcgcga acttcctgag acggcgttct
    16621 ttccgttgcg atcgtttcga tcgcgaactg gcgcaacgaa taggcggaag gcgcgccaac
    16681 gtcgggatgc aggagggaat ctccaggttt tcccgcggtc ttcatccgtt ctttccagcg
    16741 aaaaaagggc tttaaaaaca gggaagggag ataggtgttc cccgagatgt ccgccttcgg
    16801 gtaggtgaac agcacgaagt cttcggtcga tcggacggcg ttcataaagt ctcgccgctc
    16861 tttcttgacg atggtatcgc gcatcgaact gctcgagggc gcggccgcgc ccgttcctcc
    16921 cgtttgctct tttggccatt gggcgcgtag aaacacggaa ccggaggcga gcagcggata
    16981 gttttgatcg gtcatcccga cgaacacctt aacccgcttc tcccgaaacc gcgcatcctc
    17041 caggtttgac aggtacaccc gattgtcgat ccgcaccgat tggcggtacc cggagagatc
    17101 gacgaacagt ttcagatact tccagtattg ccgttgggat accggtttgg atccatcttc
    17161 ggaggggtcg atcgtataga gaaaacggtt cagctccggc aggacctcgt tcaggaagac
    17221 ccgagtcgcg ttctgttctt cctgagtatc cgacagcagg cccctcttcg aatagtcatc
    17281 gctcagcttc tgaaagtata gaatatagcc ctctttcgcc gttggggcgt tttttgactc
    17341 gaagaaggaa agcttggaga acaacattcg aatcaattca ttggcgtatt tgagccggtt
    17401 gcgttcctcc tcagcctgtt gtagggcttc cccgtcgtcg tcgagggcgc cggcgcttcg
    17461 cgctttccgc tccaccaatt tggtgtatcg cgtcaaccgt tcgttccaat ccctctttct
    17521 ccattccaaa gtgacggggg catcattgta gaaggcgttg gaataggcga gtttcgctcg
    17581 catcgccaac gtttcgaact ccgggaacgg tagtgactcc ggcaattcca cgaatccggt
    17641 ctccagaagc gctaacatcg aatccggaga aaaaccgccc gaaaaggcgc gaaacggaag
    17701 gagaagcttg ttcacgttta ggctgcgatt aaggggcaaa tcgccttcaa atcggaaggg
    17761 aaccccgttt tgcgaaaacc cttcctttaa gagggggagg tactttcgct cgtccctaac
    17821 gacgatcccg atgtcttccg gaacatattt tttttcctga atcaactgct tcacccagtt
    17881 acacgcgtag atcatttctt gatgggggtt tgagcattcc aggatatcga aaggtgtcgc
    17941 ggaagaggcc gagctcgaag cgcttatgag agaaggcggt ttatccaaca ggttgagcgt
    18001 gtccttcagg gagccagaca gtttgtcctc ggccccttcc ggcgaaatcg tttcccagcg
    18061 gtggaaggtt cttagatggg acggttccgt ttcgaaggca tcgaaggaaa ggtccatcaa
    18121 agacccatcc gggttcaacg gcgcggagag gtagacctct tcgaaaaaac agaagaaggt
    18181 gtagaagaac cggcgcgtct tcggtgtcag gtcgaagaac ccatccagga acaatttacg
    18241 atagggaact tcttgaggtt gcaccgcctt cttctctatc aaatccaaaa cgcggtagta
    18301 ggcgtcctcg accgtgaaca ggcgcttctc gcgcagctga gtgtcgatga tcctgtacag
    18361 ccccgcgaac tcttttagaa gcgtgtcttc ggtctgttcc atctcgccga gcagcgcgga
    18421 cgcgtcatgg tctttcagcc aagagtagaa ttgcaggaac gcgtcgatca ttccttggcc
    18481 tctctgcagc agcgggtaca accgatgctc ctcccgcacc ttcccggcga cgaagccggc
    18541 taggaaactg cgattgatgt agaagccccc gtcaccacgg ttgagaagga actgaaccac
    18601 gtattgatct aacaagagaa agctgtcgtt gaaaaaattc ccgacaagcg caaggaagcg
    18661 ttcgcgtaaa ttgcggatga aactccctga aggcccaagc accaggtacg agaaaggatc
    18721 ttcccgatgg gcttcggcca ctcgattcaa aatgtgtcgc gttttgcccg aagagggtat
    18781 gcctttgact atgacgatct tggccatccg aagttttctc ctttgttttg tgtttatcga
    18841 aaaacgccgc tttttgttaa ggaatcggtc ttgagttttc gtatcattat accatagcct
    18901 acggcccaca caggatgttg acaaacgcgt gttcatggta tgattcagag acgcgcaaca
    18961 aaaaggaggg aggaagatgt cgagagaaaa acggaatatc ttgttcgtag tactgttcat
    19021 gtgcgcttta ctgacccttt ttgccgaaag gcttccctgg gatgaccaaa tcatctattt
    19081 cgtcatgatc gaccggtttt ggaacggtga tcccttgaat gacgtgcaga ccccggacgg
    19141 tgtcgaagcc ggtgtggaaa actcaaagta caatggcggt gacttgaagg gactgatcga
    19201 gcgattagac tatatcaaag agttgggagc cacggctatc tggatcacgc cgcctgtggc
    19261 caatcagtgg tgggatggaa gcgttcagta cggagggtat cacggatact gggcgcgtaa
    19321 cttcaaggcg atcgacgaac acttcgggga cctcgagctg taccgggact tcatacaagc
    19381 ggcccacgaa aagaacatcc gcgtgataca ggacatcgtg gtcaaccatg tcgggaatta
    19441 cctcatatac gaccccgtca ctcttatggg ttctgtaaac gagcagagca ttccgacgaa
    19501 aaagcccaca cagtacccgt ttgatcaaaa cgactactac gacccggcgc aaagcgcgtt
    19561 gaacatctac cactggcctt cggagataga gaaccccgac cgtttcaata ccgcgctttc
    19621 ggatctcgat gatctgaaca cggaaaatcc gatcgtgatc gaggctttga aagatagcta
    19681 cgggttctgg atcgaccagg gcatcgacgg gtttcgcatc gacacctcca tttatgtgga
    19741 agacgccttc tggcctctgt ttttggaaga tccggatggg atgtacgctc gggcgtcgaa
    19801 aaacgacaag cccgacttct tggtctacgg agaggcatgg gtcacgcccc agccgtacga
    19861 ggataccgga gagcgggtac tctcgaacta ttacgacata ggttacaaca gtatgctgga
    19921 cttccctctg atgactgagc tccgtcgcgt gtttaaggag ggtaaaccga cgcagatgct
    19981 ggagtaccgg cttcagcagc gggagaacta tttttccggg aaacgcctga tcaccttcgt
    20041 agataaccac gatatggaac gatttctgaa aggcgtttcg atgcgggacc taaaacaagc
    20101 cttactcgtg attatgacga taccggggat cccatccatc tactacggta ccgagcaggg
    20161 gttcttggaa accagggcca cgatgttcaa ggaaggtttt gaatccggag gcgtcgatca
    20221 ctttcgaacc gaccatgaac tctaccagta ccttgacaaa atcatacaac tcagaaaaga
    20281 aacgcctgct ttccggtacg ggcgggtgac ggtggttcat tccgacccga taggccccgg
    20341 ggcattcatc tatcgggttc aggattcaaa ttcgacgtac ttcatcttcc tcaacacctc
    20401 ctcacagtca cggtacgcca cccaaatgga gatgggagtg gccgaaggca cggtactcga
    20461 gccgattttc accgaaggga tcattttcaa aaacgtcgtc gtcggtcaaa acggacttct
    20521 gaactacctg atcgcgggga agggtttggg ggtgtttcgc gcgaccgatg agacgcaaga
    20581 agtcaaagga ttcgacatcg atgtggcgat gacctccata cgcgagaacg aaatgatacg
    20641 cgaagacttc gtcctgaggg gcacctctcg aaacgccgcg cgcgttcggc tctatgtcga
    20701 tgggagcgag agagaatacg ccaccgtcgt tcccgatgcc gaaggccact gggacgtgcc
    20761 ggtccgaatt tcggaattca tctccggagc gcatcggctg ttcgttcgcg cttacggcaa
    20821 gaaggccaca gaatccgtct attctcagac tatgacggtc tttttcgaca tccccaccgt
    20881 tcatttggtg gaagtgacgg accccgaagg cgatgacaag ggcccgacgg gagactacgg
    20941 ctatccgaaa gacctcacct tcgagcatca gcaggatgtg ttgcacgtga agctgagtca
    21001 agtgggcaga atgctgcgta tggacatcac gatgaagaac ctcacggacg tttggtcccc
    21061 cacgaacctc ttcgaccacg tgacctttca aatctttttc gatcggccgg accgaacagg
    21121 ggcgctggcg ctgcctttcc aaaacgcgac gatgccggag ggtatggatt gggattatat
    21181 gatctacgcc accggttggt cgatggcgct ctacgaatcc gatgaaaaat cgggcccagc
    21241 ctattacggc acaccgatca caccggctcc gacagttcag gtggacaaac agctcaaaac
    21301 catcacactg ttggttccgt tggaggtgtt gaaaaccgac cggctgagcg ggtggaagat
    21361 ctatctaacc acctacgact atgacgggat cgaggccgtg ctccgaccta tcagccccgg
    21421 aggtggcgcg tgggcgttca ccgggccttc cgctctagca ccgaaaatta tggacttcgt
    21481 tttaatcgat attccgtaag gaaccaaggg taagtaagct ggcgagcgga aacgaggctc
    21541 gccggcttat tcttcggaga atacgatccg cgccaactga taggcgagca tcgtgaagtc
    21601 gtagtcgtcg aggttgctga gcacgacgat cgtgaggcct ttatccacgt aacgggaaag
    21661 gtgcgtggat gagccggcgg tggcgccgtc gtgaaaaacg agacggtacc ggacaccatt
    21721 gaacggcgga tactcgatga agacgccaag cccgttaccg tagacctcgc tttgaggggt
    21781 ttccatcaac cgaagggttt cgggtttcaa gacctttccc tctgagagcg cgacggacca
    21841 tttgagaaga tctcgagcgg tagagcacaa cccaccggcg ccgtacggga tgtctcgatt
    21901 gacccagggc gcgggctcca ctcggtcagg ttggacggaa agtctgtggc acggaatcgc
    21961 ccaatggtca tcgtagacat tcggatcata ctcggtgtcg atcatcccca agggtatgaa
    22021 gaaggtctct ttcagatagt ccggataagt gaggccggtg acgctctcga tcacggcgct
    22081 gagaatcgcg tatcccccgt tgctgtagta aaacccctct ccgggctcga atctcagagg
    22141 ctgcgctctt aaataagcga gcgtcttgtc cagggtggtg tctccgggga agcgttggat
    22201 acggtcgttc aggtcgggcg aggtgttgag tagcgtcacg atgccagagg tatgggtgag
    22261 gagctggtag agcgtaatct gtgggagagt cgaaaacgcc ggcaagtaga gcgacagcgg
    22321 gtcctgcagg ctcagccggt tctcttcgga gagtttgagt atcgcggcgg cggtgatttg
    22381 tttcgtgata gaggcgatcc ggaatttcaa ctcgggatgg atcggtcgcg tgtggtcgca
    22441 atcggcgaat ccgtatccct tttcaaagag gatatcctct ccgttcgcta ccagaacgac
    22501 gccccagaac cccatccagg tgtgctcctc ttcgcaccgc ttgtcgatac gttccgcgag
    22561 cgtgggttgc gcgaaaccga cttgggatga gaactgcaag agtatgagta tgacgatcgt
    22621 caacatcttt cgaaagtttt ccactgtcct ctcctctcct tacgctgatc gctatatatt
    22681 gtattttcgg cactgtatag aatacggaac gggtttggcg actcgggtta tcgccgaaac
    22741 tgccagcgca ggaaatcggt gacctcctcc cacgcctcgc gaacttcggg aacgaagtct
    22801 ccgaataact ggaaaacgtg gaacatccct tcgtatcggg tgagcgtcac atcgactccg
    22861 gcggctttcg ccctttcata gactatgagc gaatcacttt cgattatttc aagggcgcct
    22921 acctggatca gcatcggtgg aaaccctcga tagtcgccaa aagctggtga gaggtacgga
    22981 tcatccaatg gatggccgcc gatatagaga tttcctatcc attgtccgtt tcccgtgggc
    23041 ttttcccagg ggaacaaacc gaatatcggg tctttgtaaa ggttgtctct gtaagagtct
    23101 ccggaggcgg tcatatctgt ccagggcgac atacacacga tggctttcgg cattttgcgt
    23161 ttctcatccc gaagtttcaa ggccaatgcc aaggaaaggt ttccgccggc gctgtctccg
    23221 accacgatga tgttttcctc gcgataaccc tgctctaaaa gccaattcca aaccgcttcc
    23281 gcgtcttcca aagccgccgg gaatgtatgc tcgggcgcga tgcggtaatc gaccagcgcg
    23341 accgatgcgc caccgctgat tgtggagtac cgtacgccga ggtcgcgata gatgtcgacc
    23401 aaacccatca gataagcccc accgtgtaag agcaggatca cgcgctcagg agtcccgcgt
    23461 ttttttacaa gcagttcaac cggaacgcct tggacgatgt atttcatgtg agagaaaccc
    23521 caaggcgcct tccattgttt gtgggattga gcggaggcgg tgccggtatc gatgatcccg
    23581 gcgcccctcg atttcaagct ccaaaccatt atcctcaggg cttcccgaac gacgacaccg
    23641 gacagggaag ggccgggaaa ggttcctgaa aacgcgaacc cggttaaaaa agaaaacagg
    23701 agcaaaaccc agattccgta ccgaaaggga ttgaacggcg ttttgttttt cataacaaaa
    23761 acctcccgtc tcaaaaggag aaaatgcgat gctcacgatt tatcatatca gattgagcca
    23821 cacttttcca ccgtggaagt aataggcggg catcgcggcg taggcgccct ttcttttcgc
    23881 ggcggcgcga tacgcataat aaaaaaaagg gttcacacgg agaacgcaga gggcacggag
    23941 gggagaatcg cgttgggagc gcctgggaac gcccacttcc aggttgccag gttttgacgg
    24001 ggtcgtaaaa aatacaaaga gcgtatcgcg agacttcgtg ggcctccgaa gtcagcccgc
    24061 tggttacccc gcgataacac acgctgttgg gcgttcccag gagttctcag aaaggcgagg
    24121 cccgattttg gaagattgaa atgatttgat ttacaccgcg ggcgccgatt gttcgatccg
    24181 ataccctttc aggttccgct gttcggtgtc gatttcgatc ccaacgtagt acaggttctt
    24241 tccacggtac ttttctccgt accctttttc gcggatttgt tgcagcgctt tttccgctgg
    24301 cgctttcttc aattccatta tgtacacgtc ttcaccgtag gcgagaacca agtcgctgcg
    24361 ccccaggtgc gtcgcttctt ccgccttcgc ctctatcccg catccccaga atagagtcag
    24421 cagtacaacg tgatagaagc gctctaaccg ctcgggatga ttgccttttt gctcttcgcg
    24481 tttgtggtag agcgtgtacg gaatgctcgc gaaggttatt cgcatctgtt cgaagattat
    24541 tccgaagtcc cttttgtcca atccgttcag gattccgttc cggatcgcgt cgttcgtctg
    24601 gggttccagt ccgtatcgat gaagcagcag gagttggctg aaggcgtctt tcacttcttg
    24661 attcgggaaa tctaactcat accctctctt ttcacgatat cctttgaagg tcaaataccc
    24721 cgattgcgtc agaaaacttg ccgccggcgc cgcttctatt tcataggcgc tgaataggct
    24781 ttcgcttatt gggtactcca ttagttcgtc tattt
//
LOCUS       DAOPXN010000007        24621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2010_length_24621_cov_5.357038, whole genome shotgun sequence.
ACCESSION   DAOPXN010000007 DAOPXN010000000
VERSION     DAOPXN010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_24621_cov_5.357038"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PKM49_00845"
     CDS             complement(<1..1430)
                     /locus_tag="PKM49_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNY81392.1"
                     /translation="MNIEKTLHRSGILYQVQKPSRYIGHEWNRSKKPVEKGDARFLLV
                     FPDLYEIGMSHLGLRVLYDAVNRAPGMMAERAFLPWVDMQRKIDDGVIPLYSLETFST
                     IDDFDAVGFTLQYELSYPGVIRFIELANLSPFSHDRKDEDPILIAGGPCVYNPEPLAE
                     ILDAFFIGDAEEAIVEIGECLRATKGERKVKRLSELATIPGVYVPRFPPASPVRKRIV
                     PKEALNRVGDWIVPFIPIVHERSILETQRGCTRGCRFCQAGMIYRPVRESPLETLTRM
                     VIARTEDTGYEEMALLSLSTLDYSQLSELIESSRAYLDKKMVALSVPSSRMDQFSMQI
                     ANQIAGVRKTGLTFAPEAGTQRMRDVINKNLSEEEILATAVAAKNGGWTRLKLYYMVG
                     LPTETDEDIEGIITLTRKIKEVGFRKINVSVAGFVPKPHTPFQYCAQKTVDELHRILH
                     ALLDIKGVASFELHKPEMSWVEGVLAR"
     gene            complement(1427..2896)
                     /locus_tag="PKM49_00850"
     CDS             complement(1427..2896)
                     /locus_tag="PKM49_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HNY81393.1"
                     /translation="MTSAIKYGCFYVFLGVSLLAFFLYVYPRYEYDQKASDIAIGFEQ
                     AYTGDISGLFTVTPIQNTGLKGERVVFVYDFSVVPEANLERTLKGRAGEKVIFFERPY
                     PALKPETLASLLDRYGIVAGFLELNPVSNFMRKVLLARSKSGREGTFRVHTIKPAEVT
                     NLRLTYEMVLRRWLRAREERSIDFFWVQPLPSSLEVSYDEYGVTLLRLFHTTGSLSPV
                     PVSMNVFFKIMLAIGSFGLIIFYSPVIAIACAVFLSIYGIFNGFADTWLYLAGLTGTF
                     GITGVFREMRKLEFNTSLKYIATIIFALFLGVTVNALSYSHESVTGLLQPHGVKLLTF
                     YLPMLVFIREFISYGLQGLKSRLHWSDFVLVIGLVLAILYSLLRSGNDAFVTNFERQL
                     RDSLEMLLGVRPRFRELIGIPALWLYFRDKHHGFGRYAFIIPVLGVIGLCSIVNAFQH
                     VHTPITIIFLRELLGIVIGTAAGMLIGVFLPAREEGESV"
     gene            complement(3179..3364)
                     /locus_tag="PKM49_00855"
     CDS             complement(3179..3364)
                     /locus_tag="PKM49_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNY81394.1"
                     /translation="MSVVKVDQDACIGCGVCSSLCGEVFDLADDGKAFVKEDADTDLP
                     CVEDAVNSCPVSAITVE"
     gene            3470..4276
                     /gene="rsmA"
                     /locus_tag="PKM49_00860"
     CDS             3470..4276
                     /gene="rsmA"
                     /locus_tag="PKM49_00860"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNY81395.1"
                     /translation="MKTADWIDPHGLRLFKKYGQNFLSDDRIAMKIVEVGETQSTDRL
                     VEVGAGCGTLTHELLRTGLPVHAFEIDPRFEKVLRSRFGQLPNFHLYITDFLNYKPES
                     YWQEGEWVFFGNLPYNVSVAILRKALFDFRSVRRWVFMFQYEVALRLCAFPGSKDYGS
                     LSILVQSVTRPQIAFKVSKSHFKPSPKVDSAVVTLDPIDPEIPEGERDAFSAFVRKGF
                     ANRRKMLKNNFPEEGRLPHWLESLSKPLTTRAEELGVTDWISLYKLWKQG"
     gene            complement(4297..4911)
                     /locus_tag="PKM49_00865"
     CDS             complement(4297..4911)
                     /locus_tag="PKM49_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81396.1"
                     /translation="MEISRVNGIGNDYAYLRNHRAPARVEAAPVSPSSLANSPWGPAA
                     STGTPGPLNLADRNAVENAERYRPKDPMLKLAKRMGIVECQTCKNRQYADVSNDTGVS
                     FKNAQHISPEAAFAVVSAHEQEHVSIAQQKAAANDNVSVSSSVRIFTSICPECGRVYV
                     SGGETRTTTVTRPEQPEVEKKESIPYLTESSFTPKTGNRFDAAA"
     gene            complement(5351..6784)
                     /locus_tag="PKM49_00870"
     CDS             complement(5351..6784)
                     /locus_tag="PKM49_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HNY81397.1"
                     /translation="MNKESLFREGPKVANIGLERFNETLKEQGVPTYQQPWTPDHTPL
                     SLYLRVKEVERDLVLAERIGNANRETLTRLQTSLPILHGMGLAKDVIPGLSGRMLLHA
                     GPPITWERMSGPMRGAVTGAILYEGWAKNVEEAQGMAARGDIVFDPCHHHQAVGPMAG
                     LISPHMPVFIVKNTAYGNMAYATMNEGFGQVLRMGAFSERVVNHLRWMRQTLYPILCR
                     AIDTLRELDRPINLKSIMAQALHMGDEMHNRNKAGTSLFIRQIAPTLAETAEDTSKTA
                     DALRFLDSNDHFFLNLAMAGAKASLDACAGIPYSSIVQVMARNGTDFGIRLACLPDRW
                     FTCPAAVPKVLLFPGFTQEDVNPDIGDSAIMETYGIGGFALATAPAIVQFVGGTPQDA
                     ANYTIEMYTICHSENRDYTIPALQFKGTPTGIDLLKVIRQNTTPILDTGAASKEAGRG
                     QVGAGIVRMPSEAFIKAAQAFLDNLRT"
     gene            complement(6789..8330)
                     /locus_tag="PKM49_00875"
     CDS             complement(6789..8330)
                     /locus_tag="PKM49_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty-acid metabolism regulator protein"
                     /protein_id="HNY81398.1"
                     /translation="MKATKVIKGRYADSITLMLIAEELKKQRGVSDAVMNMATPANID
                     IMREGGFQIDLESYSPDDIIIGIDAEPENAKAFLKNAEDWLSNPPWQKEGRDKTAEAP
                     SEPPLDTNLAIISVSGKYAAIEAFKNLERDRNVMLFSDNVTKAEEIALKQVAHRRGLL
                     VMGPDCGTAVINGVGLGFANRGKTGIVGMVGASGTGIQETYTLLQKRDIGVLHAIGTG
                     GRDIHDEVGGATFLDGLQILWEDSRISIVVCISKPLSDSMKNKISSKIESLSRTYGPK
                     PVAHCYVGDKRRAHQPNNYFCTGFEETARVVEALVTQTPVDSVLPRMAEERKIKLERL
                     DETLRHRGSPGPVLRGFYTGGTLCYEAQSVLSEILPGVRSNAPIDRELIARDPFHPEG
                     HCVIDYGEDAFTQGRLHPMIDPSLRNAQMKKQSMQPEVGVVLFDVVLGFGCHENPGEE
                     LAQVIAGTYAHGKGNRFFFCSITGLDEDPQDARKQRRLLESAGAIVCASNSEAAYIAG
                     HLLKR"
     gene            complement(8361..9158)
                     /locus_tag="PKM49_00880"
     CDS             complement(8361..9158)
                     /locus_tag="PKM49_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81399.1"
                     /translation="MTVPKTLFFLVLLGILLVLAAFQNQPISSFVKTAVLNATVSLSS
                     VRDTIDHQLNKLYGKNQLAPSESVAPELSLLKGLRQTVDTIFKTEFEDLLGSVETLRK
                     LKIGGLYPIVFLLRTMGTGNQETWILYSEEPVPGNAFVVSIREGALIGRVHEVNGNIL
                     TVIPLWNQAFSTQVRVLSADRQIRDLAYLEDGKLLNFNPSFPFQPGDEVYLSDYEPGG
                     YALQRYGWNRLGTVGALLNEDIVEYYEIIYRFRRDRFLDERYFLVVS"
     gene            complement(9170..10243)
                     /locus_tag="PKM49_00885"
     CDS             complement(9170..10243)
                     /locus_tag="PKM49_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNY81400.1"
                     /translation="MSKNLSGMDIGADYTRLYSIVGGKTEFFVEEPTILLYNEFGELK
                     AFGTEAKEYLGKTPPFYYFQNPVDESGIHQFEHAHVFFERFLKKNAVLKGWSKPSVCF
                     NSPLGSTPVQVQAGIELLEDLGFAQVYTLPTPVGAALGTDLDLRDRRGHLIVIFGASH
                     TEIAVVSLFKIVSGKHLQIGLRHIYQQFVYFLREQHALEISERMAQALVTDYLVLSEE
                     GAQSHPAPSASADLPSAHMEKEFFAVGIDLTTHLPKKVKLKCSTLFALARPFLDRLIL
                     SLQEVFETVGPEIVKDILERGVIFAGGGAYLKGLPAYFEKKTTMRLLIPENPSQCIAR
                     GNWTYLHALARKELPENLFPKER"
     gene            complement(10256..11527)
                     /locus_tag="PKM49_00890"
     CDS             complement(10256..11527)
                     /locus_tag="PKM49_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNY81401.1"
                     /translation="MAVIKPFRAVRPPKHLVGKIASKPYDVIDSEEARAEVVGNPYSF
                     LHVVKSEVDCDPSVSLYDPQIYAKARANLESFIEKGYLVPDREPALFVYRHTWKGPKC
                     PFPEGQTQTGVLGAFSCEEYQKGSIKKHEWTRRDKEDDRLNHILTTKAQTGLVFLAYK
                     HQALLDTLVQETLRSEPEYRFSDELDVVHELFVVNDPKRIAAFQRAFEQLPELYIADG
                     HHRSASSSRAQQSRKAANPRHTGEEEYNFFMAVAFPDNQLKILDYNRVVKDLNGRSEE
                     DFLKAIQTNFSVQKAPNRPYRPLKKGEFGMYLGNQWYVLGAKPGSFPAEDPVKRLDVS
                     ILQENLLNPLLGIQDPRTDKRIHFVGGIRGLKELETLVDSGNWKVAFSMFPTSLEDLF
                     AVADQGKTMPPKSTWFEPKLKSGLVVHFLDS"
     gene            complement(11542..12063)
                     /locus_tag="PKM49_00895"
     CDS             complement(11542..12063)
                     /locus_tag="PKM49_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNY81402.1"
                     /translation="MAPAGPDLKACADGEILFFQGEPDTRLYRLVGGKVLLYTLFQKP
                     RIIAVVKESQFFGFAPLCSGYHLFNAEAVGAARVETFEYDSKDHVFLKSYYTGLCRDL
                     QQYGREELWGKPETEAAEMWRLVSELHTFKEPFLVRFFPFQTVTNTLKTWVSEKKIQP
                     DRQKGSYRLVKEP"
     gene            complement(12242..13654)
                     /gene="cysS"
                     /locus_tag="PKM49_00900"
                     /pseudo
     CDS             complement(12242..13654)
                     /gene="cysS"
                     /locus_tag="PKM49_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
     gene            complement(13680..15122)
                     /gene="gltX"
                     /locus_tag="PKM49_00905"
     CDS             complement(13680..15122)
                     /gene="gltX"
                     /locus_tag="PKM49_00905"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNY81403.1"
                     /translation="MVRVRFAPSPTGFLHVGGARTALFNYLFAKNQNGVFVLRIEDTD
                     LERSTLESEQSLLRTMKWLGLHWDEGPDIGGPKGPYRQSERLDIYRRMAYALVEKGKA
                     YEAYVLPEELEAMRGKMLENKESPRYSHETIAQYDTPERRQEFAQKGWSPVVYFKMPQ
                     KEYVLNDRIKGRVVFKGGTTGDFVIFRSTGIPVYNFAVVVDDALMEITHVIRGDDHLT
                     NTLRQLALYEAYQMPLPEFAHVSMILGPDGSRLSKRHGATAVENFREMGYLPESLINY
                     LALLGWSHPQENEICALNEMIRAFSLERVSSNPAIFDSGKLLWMNGMYIRQLTPEALL
                     EKAVPFIIETGYIAPENVDVNKKWLLEAMKAIQNGIHQLDEVGELLSPFFTDPIPTAE
                     ALRSLEDDRLSAAFKLLYERFDSIPSWEINEIISVIKAVVKDTKVNPKPFYHTLRLAL
                     TARDTGPELVHVVHLLGRNKVLNRLGRLEV"
     gene            15801..16703
                     /locus_tag="PKM49_00910"
     CDS             15801..16703
                     /locus_tag="PKM49_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81404.1"
                     /translation="MKRVLMVSILVFVLAVSSVFAFNLGKQKCWYLIQPAQQVIGVKA
                     CVPVCIDPGVLDPGLYQKLVALYQEAVAKGAHANFLSVLQILIAQHTNQQIGVVPAAS
                     QSQYFYTHTFSDKLDNWAFYRAGQIQSWQHVTSGKTRYLQLNATGSAMIQAEKIIPIP
                     AYMKGKAVVVTASVMVDLKAAALNSPTDFAAAGVVAHLYNRDYTAYSELPPSQAKAVL
                     WSTSEYPTRHSSFLYPDEEIIFASDPYRTLTGVNIIFWPDVTANMECVKIGLIAFGEG
                     DSASTQADIWISDVRVMLADAEYR"
     gene            complement(16789..17337)
                     /locus_tag="PKM49_00915"
     CDS             complement(16789..17337)
                     /locus_tag="PKM49_00915"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNY81405.1"
                     /translation="MKGTGFCGINCHTCPIYQATVEDKPEERAKLAREWNKTHQTTYK
                     ASQMICLGCKSNRPFAYSDECEIRECNIRRGLKNCGGCLEFLCKIGREFWKTMPEEYE
                     NLKKIHRLLFKDVADSVSEFEEPEEEGIRSISKRGALPYEEHTQKNHQEHAINKPVKK
                     KAAPLVKKNNPSKKKNNATQDD"
     gene            complement(17380..17835)
                     /locus_tag="PKM49_00920"
     CDS             complement(17380..17835)
                     /locus_tag="PKM49_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81406.1"
                     /translation="MTKKEPLHHTREIDPKNAAGEQPATPESLFNLRRMLRNKDDAQV
                     LQAIEKIEQMNQIGKELLADIKNYLWQRGNTANNLTEKMARLFRRFDPEGYETFILLR
                     DKSMGNLPSKEKRLSSKSAFEEEIKDFGDPRVELVGELDVDDGDEEESD"
     gene            complement(18250..19464)
                     /gene="lysA"
                     /locus_tag="PKM49_00925"
     CDS             complement(18250..19464)
                     /gene="lysA"
                     /locus_tag="PKM49_00925"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNY81407.1"
                     /translation="MLRYENGWFVEKVPVDLIAEKYGTPTYVYSAAQMDAAIMRVMES
                     VEGLPATVFFACKANNNPLLLNYFHQKGLWMDVVTMGEYQIALRSGVKPERIVVNGNG
                     KTLLDIETWLNDGVFAINIDSKEEYGLIVENGLTGERGTRFFLRVNPDVDSRTHPYIS
                     TGLKKNKFGIPMEEAQKLLQEDHLPVSGIHMHIGSSIQEIAPFKEAMEKLLSFKERNP
                     QIQMVNFGGGWGIDYGHAGSGFDLNRFREEIGPLLRQFRCPIFLELGRYIVASAGLLL
                     SKVLYLKQTPYKRFLVCDANMASLIRPALYQAEHMVVPLIQKWEKRQKMTVDVVGALC
                     ETGDILAYNRSLEELIPGEHIAILDTGAYGYSMSSNYNGTFRPAEVLVNDEKTRLIRK
                     RETVDDLLRLID"
     gene            19588..20622
                     /locus_tag="PKM49_00930"
     CDS             19588..20622
                     /locus_tag="PKM49_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNY81408.1"
                     /translation="MALIKRVTFKLNEVFYQKPFHITNSVRSSSKVIAIALELDNGII
                     GRGEASPSFRVNGEIETVLMGFETPISEMLRNREVQRYRPLFDTLDKFSRSAPSLKAG
                     VQYAILDAFSTLISTPVYEILGGAKEYVETDFTISIDSLENTVQDAKKASEKGFKILK
                     LKVGEDLPSDIARVLAVQEALPSMHFVVDANMGYTPKQAVTFAEAMVSNGVKMDLFEQ
                     PVWSDDFDGLRFVRQHSGIPICADESAKTRFDALRLIREECVDYINIKLMKSGISDAL
                     AIVELARSAHVGLMIGCMSESGVGIAQSVHFACGTGAFTHHDLDSPFLLKETTPYKYR
                     TEGCRLLPIG"
     gene            20658..21722
                     /locus_tag="PKM49_00935"
     CDS             20658..21722
                     /locus_tag="PKM49_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNY81409.1"
                     /translation="MDVILESCASYEEAERQLPQHLKRYEGLFWKNEKVWVKPNMLSA
                     RSPEEGVTTHPTIVRGVLRFLLDLGVKPVVSDSPGVGNVRKVAVACGISQVCQELAVP
                     LIEMEEFVEKDGDVFKQLRLGDLIDRVDAVVNLPKLKTHGQMVLTMGVKNTFGCVLGL
                     NKPAYHLRAKDYHLFADLLVDVHQLVKPVLTVMDGIDGMEGNGPSNGQKKRLGLLASC
                     KNAYALDHAVAMRLHVSPDKVYTLARAKARGLIPEYKIHGNWRNSVKMPDTVGLTEIV
                     KMPLWLQELTHHWARVPVFNTPKCIQCKICERHCPANAITVDKQHIDYKKCIRCYVCH
                     ELCPEDALMLKRRLWKAGGR"
     gene            21719..22282
                     /locus_tag="PKM49_00940"
     CDS             21719..22282
                     /locus_tag="PKM49_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNY81410.1"
                     /translation="MKDKRLLRTLFWTFFKIGCFTFGGGYAMIPLIEREVVDTHHWVS
                     REEILDILAVAQSLPGAIAINSSTLIGYKMAGKTGALISTLGMVLPSFLIILIIAMVF
                     SAFQENEWVRTAFVGIRAAVVGLIVIAGYNMFKAAVKDKIGIGILVVTLFLLFLLDVH
                     PILLLIGGAGFGILLYILFPEKGRGSR"
     gene            22279..22848
                     /locus_tag="PKM49_00945"
     CDS             22279..22848
                     /locus_tag="PKM49_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNY81411.1"
                     /translation="MIIELFSIFFKIGLFSFGGGYAMIPMIRNEIVSRGWLTAEGFAN
                     IVGIAEMTPGPIAINMATFVGFKTIGFFGSVAATLGVVMPSVLLILLISKIFFKYQSH
                     PIKQGLFYGMRPVIVGLILSAAYFMAETSLFIVKPGEWTGNFLSQVDWVSVGIALLTV
                     VGIRKYKLHPILLLGVGGAIRIGLKFVGV"
     gene            complement(22964..>24621)
                     /locus_tag="PKM49_00950"
     CDS             complement(22964..>24621)
                     /locus_tag="PKM49_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY81412.1"
                     /translation="ATNEELIATNEELNAVNQTLERSFIEIESKNAALERIKAEFEEL
                     LQQTGAVVWQVDLNGTFVSLRNLNPEVNGFTEEEMLGKMSVYDLHPEETRGEFKAYIT
                     EVMRSEKTLWNFEHPVSTRDGRKRWVLSVWFPAYDERHRSVGYRGINIDITAKKALEE
                     ALIKSKEEAQAALKAKSAFLSNMSHEIRTPMNASMGFTHLLLETEENPEKRKMLEQID
                     ASNRYLLNLVNKILDLSKIEDGKIRIREEDFMIGEMLDRIVDNFSHLARAKALDFFVK
                     REPEMPVIVQGDELRLEQILNNVLSNAIKFTEKGAVTFEMAATPIENSEHRMLFKFKI
                     ADTGIGIEEKDFQRIFDKFEQAESYLTKKYGGTGLGLSITKELVDRMKGFLRLESSTG
                     KGTTVVIEIPMTVDGEAYTKKSENGGPVTYDLEGVHILIAEDNEITQNLMKRIFQRHG
                     ISVDLAENGQKALEMLQQNRYDLLLMDIQMPILNGIDTTKLIRREERFKDLPIIALSA
                     FIDQEVEAPALESGMNGFVTKPLTAQTIHHLITTYLLPLKEKS"
BASE COUNT         5459 a         6495 c         6461 g         6206 t
ORIGIN      
        1 cgggctaaga ccccttcgac ccaactcatc tctggcttat gcagctcgaa agaggccacc
       61 cctttgatgt cgagtaacgc gtgtagaatg cgatggagtt cgtctaccgt cttctgggcg
      121 caatactgaa acggggtgtg gggtttggga acgaatccgg ccacgctcac gttgatcttc
      181 cggaacccga cttccttgat ttttctcgtg agcgtgatga taccttcgat atcttcatcg
      241 gtttcggtcg gaaggccgac catatagtag agtttcaatc gcgtccaacc gccgtttttc
      301 gccgctaccg cagtcgcgag tatctcttcc tcgctcaggt tcttgttgat cacatcgcgc
      361 atcctttgtg tgcccgcctc cggtgcgaag gtgagcccgg tcttccttac gcccgctatt
      421 tgattggcga tctgcatact gaactggtcc atccgagagg aaggtacgga gagcgccacc
      481 atttttttat ccaaatacgc ccgcgaactc tcgatcagtt cggaaagctg tgaataatcc
      541 aaggtgctca aggagaggag cgccatctcc tcgtaaccgg tatcttccgt tcgggcgatg
      601 accatacgag tcagggtttc caaaggggac tcacgaacgg ggcgatagat cataccggct
      661 tgacagaacc ggcatccgcg ggtgcaaccg cgttgggttt caaggatcga acgttcgtga
      721 acgatgggaa tgaatggaac tatccagtcg cctacgcgat tgagcgcttc tttgggaacg
      781 atccgcttac gaaccgggga agccggtggg aatctcggga cgtacacgcc cggaatcgtc
      841 gcgagttccg aaaggcgttt gaccttcctt tcgcctttcg tggcccgcag gcattcgccg
      901 atctcaacaa tggcttcctc ggcatctcct atgaagaacg cgtccaagat ttcggcgagc
      961 ggttcgggat tgtacacgca cggccctccg gctatgagta tcgggtcttc gtctttgcga
     1021 tcgtgagaaa aaggcgacag gtttgccagt tctatgaacc ggatgactcc tgggtaagag
     1081 agttcgtatt gcagggtgaa gcctaccgcg tcgaaatcat caatggtgga gaaggtctcc
     1141 aagctgtaaa gtgggatcac cccgtcgtct atcttccgct gcatatccac ccaaggtaaa
     1201 aaggcccgtt cggccatcat tccaggagcg cgattgacgg cgtcgtacaa gacgcgcaag
     1261 ccgaggtggc tcataccgat ctcatagaga tcggggaaga cgagaagaaa acgcgcgtcc
     1321 cctttttcta cgggcttttt ggatcgattc cattcatgac ctatgtaccg agagggcttt
     1381 tgcacctgat acaagatccc cgagcggtgc aacgtttttt ctatattcaa acggattctc
     1441 cttcctctcg cgcgggtaaa aacaccccaa tcaacatccc agcggcggtt ccgatgacga
     1501 tgcccagcag ctcacgaagg aaaatgatcg tgatgggggt atgaacgtgc tgaaaggcgt
     1561 tgacgatcga acacagcccg atcaccccta aaacggggat tatgaaggca tagcgcccga
     1621 agccgtgatg tttgtctcgg aagtaaagcc acagggcggg gataccgatc agctctcgaa
     1681 aacgtggacg gactcccagc aacatttcca gagaatcgcg caactgtctt tcgaaattgg
     1741 tgacgaacgc gtcgttgcca gaacgaagca aactgtatag aatggcgaga accaaaccga
     1801 tcacgagcac gaagtcgctc caatgcaagc gagacttcag cccttggagg ccataactga
     1861 taaactctcg gatgaagacc aacatcggga ggtagaaggt gaggagcttg accccgtgcg
     1921 gctgaagcag gccggtcacc gattcgtggg aatagctcaa cgcgtttacg gtcactccca
     1981 aaaacaaggc gaagatgatg gtggcgatat acttcaggct tgtgttgaac tccaacttcc
     2041 tcatctcgcg aaagacaccg gtgatcccga aggtaccggt caatcccgcg agatacagcc
     2101 aggtgtcggc aaaaccgtta aaaatcccgt aaatagagag gaagactgcg caggcgatcg
     2161 cgatgaccgg agaatagaag atgatgaggc cgaaagagcc gattgcgagc ataattttga
     2221 aaaacacgtt catcgaaacg ggcacaggac tcagcgagcc ggtggtatgg aacaaacgca
     2281 gaagagtcac accatattca tcgtaagata cctctagtga cgacggtaag ggctggaccc
     2341 agaaaaaatc gatgctccgc tcttctctgg cccgtaacca ccgacgaaga accatttcat
     2401 aggtaagccg tagatttgtc acctccgccg gtttgatggt gtggacgcgg aaagttcctt
     2461 ctcgtccgct cttcgaacgc gcaagcaaga cctttcgcat aaagttcgaa acgggattca
     2521 attccaggaa tccggccacg attccgtatc ggtccaacaa cgaggctaag gtttccggtt
     2581 tgagcgcggg gtacggcctt tcgaaaaaga tgaccttttc ccccgcccgc ccttttagag
     2641 tccgctccag atttgcctcc gggacaacag aaaagtcgta gacaaaaacg acgcgctcgc
     2701 ctttcagtcc ggtattttgg atgggggtca ccgtgaagag ccctgatatg tcgccggtat
     2761 aagcttgttc aaaaccgatg gcaatgtcgg aagctttctg atcatattcg taacgggggt
     2821 acacgtacaa gaaaaaagcc aggagagata cccctaggaa cacatagaag catccgtact
     2881 taatggcgct cgtcatttca cccctcacct gtagaggtcc agtggtctta tcgtgaacaa
     2941 acgtatacga atagtataaa tttcagtaaa ccctaacaca tggttttcat acattgaact
     3001 tttctttcga aagagaagtc tgatatatag aacagaggaa gcacccctta ttcccccttg
     3061 aggataaata taccccccac attggagcgg acaagtctgt ccgctttttt attgatcaag
     3121 caagaaagca tatcaggcgg ccgggccgcc tgacaatgga ttgccttaac ccgaggtcct
     3181 attcgaccgt aatcgcgctg accgggcacg aattcacggc atcttcaacg caaggcaggt
     3241 ccgtatccgc atcttctttt acgaacgctt ttccgtcatc ggcgaggtca aaaacctctc
     3301 cacaaaggct tgagcaaacg ccacaaccga tacaggcgtc ttggtcgact ttgacaacag
     3361 acatttaagc acctccatat ggtattcgcc tgatattata tcacactttc gaaagggctt
     3421 tcgagggtca tttatgttac aatccccttt gaagacgcgg aggtgcgtga tgaaaactgc
     3481 tgactggata gacccgcacg gattgaggtt gtttaaaaag tacggccaaa actttttatc
     3541 agacgatcga atcgcgatga agatcgttga ggttggagag acccaatcga ccgaccggct
     3601 tgtcgaggtc ggcgccggat gcgggacatt gacccacgaa ctactgcgga cgggtctgcc
     3661 ggtccacgcc ttcgaaatcg atccgcgttt cgaaaaggtg ctgcgctcgc ggtttggaca
     3721 actgcccaat ttccacctgt acatcacgga ttttttaaac tacaaaccgg aatcatattg
     3781 gcaagagggc gagtgggtct tcttcgggaa tttgccctac aacgtgagcg tcgctatttt
     3841 acggaaagcc ctattcgatt ttcggagtgt tcgaaggtgg gttttcatgt ttcaatacga
     3901 ggtggcgtta cgactttgcg cgttccccgg atccaaagac tacggctccc tttcaatcct
     3961 ggttcaatcg gtgacccgcc ctcaaatcgc cttcaaggtt tcgaagtctc actttaaacc
     4021 gtctccgaag gtggactccg cagtcgtcac cttagaccct atcgatccag agataccaga
     4081 gggggagcgg gacgcgttct ccgcgttcgt tcggaaggga tttgccaatc gcagaaagat
     4141 gcttaaaaat aattttcctg aggaaggcag gctaccccat tggctggaga gcctttcaaa
     4201 gccgctcacc acgcgcgcgg aggaattagg tgtgaccgac tggatcagcc tctacaaact
     4261 ttggaagcaa ggttgaggcc gagatatcgg tctggttcac gcagcggcgt cgaaacggtt
     4321 gccagttttc ggggtgaagg aggattccgt cagatagggg atggattcct ttttctctac
     4381 ttccggctgt tcgggtcggg tgacggtggt cgtgcgcgtc tctcccccgc tgacgtacac
     4441 ccgtccgcat tcgggacaga tcgacgtgaa gatgcggacc gaactgctga cagagacgtt
     4501 atcattggcg gcggctttct gttgggcgat gctcacgtgt tcttgctcgt gggcggagac
     4561 gacggcgaag gcggcttcgg gggaaatgtg ttgcgcattc ttgaaggaga ccccggtatc
     4621 gttggacacg tcggcgtatt gacggttttt acaggtctgg cactcgacga tccccatccg
     4681 tttggccaat ttcaacatcg ggtccttggg ccggtatctc tcggcgtttt ccaccgcgtt
     4741 tcgatccgca aggttcagcg ggccgggggt gccggttgaa gccgcaggcc cccaaggcga
     4801 atttgccagg ctggaaggag aaaccggcgc ggcctctact ctcgccggcg cccgatggtt
     4861 tcgaagatac gcataatcgt ttcctatgcc gttcactcga gaaatctcca tgcgtcctcc
     4921 cattctcttc gagtcttatg atgagtatat caaatttcac ccgtcgaggt caacctcgtg
     4981 tgggtcaagc tatgggtgta atcaagaaac aagatcagaa tcgttggtgc gatttttagt
     5041 ccacgtggtc atcgcggcgt aagcgcccct tcttttcgcg gcggcgcgat acgctttttt
     5101 tctggctcac cttcggagta aaaatgtaaa aggcgtatcg cgagacttcg tgggcttcga
     5161 agtcagcccg ctggttaccc cgcggtggac ctcctagtta tgtcacccca taacttgacc
     5221 cacactcaac ctctttcggg cttccgggcg tctactcctt cggaccgtgt ttaagcaacg
     5281 gattagacga ggcgtttatc cgcaccccga agtgcgaaat cagggcggcc ggcccgactg
     5341 ccaatctcat ttacgttcgc aaattgtcca aaaacgcttg cgcggctttt ataaaggctt
     5401 ccgaaggcat ccggacgatc ccggccccga cctgtcctcg ccccgcttct ttgctcgctg
     5461 cgccggtatc gagaatcggc gtggtattct gccgtatgac tttgagcaga tcgataccgg
     5521 tcggtgtccc cttgaattgt agcgctggaa ttgtgtagtc ccggttttcc gagtgacaga
     5581 tcgtgtacat ctcgatggtg tagtttgccg cgtcttgcgg ggttccgccg acgaactgaa
     5641 cgatcgcggg cgccgtcgcc aaggcgaaac cgccgatacc gtaggtttcc ataatcgcgc
     5701 tgtctccgat gtccggattg acatcttctt gggtgaatcc cggaaagagg agcactttcg
     5761 ggacggcggc cgggcaggtg aaccatcggt cgggcaggca cgcgaggcgg atgccaaaat
     5821 ccgtcccgtt tcgagccatg acctggacga tcgagctata agggatgccg gcacaggcgt
     5881 ccaaagaagc tttcgcgccg gccatcgcga ggtttaaaaa gaagtggtcg ttagagtcga
     5941 ggaagcgcaa ggcatcggcc gtcttcgacg tatcttccgc ggtttcggcc aaggtcggcg
     6001 ctatttgccg tatgaagaga ctcgtgcccg ccttattacg attgtgcatc tcatcgccca
     6061 tatgcaaggc ctgcgccata atcgatttta ggttaatcgg acggtcgagc tctcgcaggg
     6121 tgtctatcgc ccgacaaagg atcgggtaca gggtttgacg catccaacgc aggtgattga
     6181 ccaccctctc gctgaaggcg cccatcctca agacttgacc aaaaccctcg ttcattgtgg
     6241 cgtaggccat attgccataa gcggtgtttt taactataaa aaccggcata tgcggagaga
     6301 tgagcccagc catcggaccg acggcctggt ggtgatggca agggtcgaaa acgatatccc
     6361 cccgagcggc catcccttgc gcctcttcca cgtttttcgc ccaaccttcg tacagaatgg
     6421 caccggtcac cgcccctctc atcggaccag acatccgctc ccacgtgatc ggcgggccgg
     6481 cgtgcaagag catccttccg gagaggccgg ggatgacatc tttggccaat cccattccgt
     6541 gcaagattgg tagggaggtc tgcaatcggg ttaatgtctc tcgatttgcg tttcctatcc
     6601 gttcggccag aacaagatct cgttctactt ctttcacgcg taggtataat gagagaggcg
     6661 tgtgatccgg cgtccacggt tgctggtagg tgggcacacc ctgctctttc agcgtctcgt
     6721 taaatcgttc caaaccgatg ttagccactt tcggcccctc gcggaatagc gattctttat
     6781 tcatcatctc acctctttaa caaatggcct gcgatatagg cggcttccga gttgctcgcg
     6841 cacactatgg ctccggccga ttccagcaac cggcgttgtt tgcgagcgtc ctgtggatct
     6901 tcgtccaggc cggtgatgga acagaagaag aatcggttac ctttcccatg cgcgtaggtt
     6961 ccggcgatga cttgcgccag ctcctcgccc gggttctcat ggcacccgaa tcccaaaacg
     7021 acgtcaaata acacgacgcc gacctccggc tgcatcgact gctttttcat ttgcgcgttg
     7081 cgtaaggagg ggtcgatcat cggatgcagc cgcccttggg tgaaggcgtc ctctccgtag
     7141 tcgatcacac aatgcccctc cggatggaac gggtctcggg caatcagttc tcggtctatc
     7201 ggcgcgttgc ttcgcacacc ggggagtatt tcggacaaga cactctgggc ttcgtagcaa
     7261 agcgtcccac ctgtgtagaa tcctcgcaga acgggcccag ggctcccgcg gtgccgaagg
     7321 gtttcatcaa gcctttccaa tttgatcttc ctttcttcgg ccattcgcgg gaggacgcta
     7381 tcgacgggtg tttgtgtgac gagcgcctcg accacgcgcg cggtctcctc aaatccggta
     7441 cagaagtagt tgtttggctg atgcgccctt cgtttgtcgc caacgtagca atgcgcgacg
     7501 ggctttgggc cataagttcg acttaagctc tcgatcttcg atgagatctt gtttttcatc
     7561 gagtcagaga gcggtttcga gatgcagacg acgatcgaaa tgcgcgagtc ttcccaaaga
     7621 atctgaagtc cgtccaaaaa cgtcgcgccg cccacctcgt cgtggatgtc ccgaccaccg
     7681 gttccgatcg cgtggaggac cccgatatcc cgtttttgga ggagcgtgta ggtttcttgg
     7741 atcccggtgc cactcgcgcc gaccatcccc acgattccgg tcttcccacg attcgcgaat
     7801 cccaaaccga ccccattgat cacggcggtg ccgcaatcgg gccccatcac gagcagcccc
     7861 ctccgatggg ctacttgttt cagcgcgatc tcttcggctt tcgtgacgtt gtcggagaaa
     7921 agcatcacgt tacgatcccg ttctaagttt ttaaaggctt cgatcgcggc gtactttccg
     7981 gaaacggaaa taatcgcgag attggtatcc aaaggcggtt cggacggagc ttcggcggtt
     8041 ttgtcgcggc cctctttttg ccaaggcggg ttggaaagcc aatcttcagc gttttttaga
     8101 aaggctttgg cgttttcggg ctcggcgtct atcccgataa tgatatcgtc gggtgagtaa
     8161 ctctctaggt cgatttgaaa tccaccctcg cgcatgatat cgatattcgc cggtgtagcc
     8221 atattcatca ccgcatccga aacaccccgt tgctttttca attcttccgc gatcagcatc
     8281 agcgtgatgc tgtccgcgta ccttccctta atgactttcg ttgctttcat agttcacccc
     8341 tttttacggc gtcccctctc tcaagagacg accaaaaagt accgttcgtc caaaaagcga
     8401 tcgcgtcgaa accggtagat gatctcgtaa tactctacga tatcctcgtt caggagcgcc
     8461 cccaccgttc ccagtcggtt ccagccgtag cgttggagcg cgtaaccgcc cggctcgtag
     8521 tctgacagat aaacctcgtc ccccggttgg aaagggaaag agggattgaa gttgagcagt
     8581 tttccatctt ccaggtaggc caggtcccgt atttggcgat cggcggaaag gacgcgcact
     8641 tgtgtcgaaa acgcttggtt ccacaagggg atgacggtga ggatgttccc gttgacttcg
     8701 tggactctgc cgatcaacgc cccttcccgg atggagacga cgaacgcgtt acccggcacc
     8761 ggttcctccg agtacagaat ccaggtttcc tggttccccg tgcccatcgt tcgtaaaagg
     8821 aagacgatcg gatagagccc tccgatcttg agcttccgca gggtttcgac cgaaccgagc
     8881 aggtcttcga actcggtctt aaagatggtg tctacggttt gacgaaggcc tttcaatagg
     8941 gacaactccg gagcgaccga ctccgacggc gcgagctggt tctttccgta gagtttgttc
     9001 aattggtggt cgatggtatc gcgaacggag gagagggaaa ccgtcgcgtt caggacggca
     9061 gttttcacga aagaggagat cggttggttt tgaaacgcag ccaaaacgag gagaattccc
     9121 agaagaacca aaaaaaagag cgtcttaggg acggtcatcc cggaaaccct cacctctctt
     9181 ttgggaacag gttttcgggc agttctttcc gcgcgagcgc gtgcaagtag gtccaatttc
     9241 ccctcgcgat gcactgggag gggttttcgg gaatgagaag gcgcatagtg gtttttttct
     9301 cgaaataggc gggcaaccct tttaagtagg ctcccccgcc ggcaaagata accccgcgtt
     9361 ccaagatgtc cttcacgatc tccggcccca cggtttcgaa gacctcttga agggaaagaa
     9421 tcagtcggtc gaggaacggc cgtgcgagcg cgaacaaggt cgagcacttc agtttcactt
     9481 tttttggcag gtgcgttgtc agatcgatcc cgacggcgaa gaactccttc tccatatggg
     9541 ccgaaggtag atcggcgctt gcggatggcg cgggatggct ctgcgctccc tcttccgaca
     9601 gcactaaata atcggtgacg agcgcttgcg ccatacgctc gctgatctct aaagcgtgtt
     9661 gctctctcaa aaagtacacg aattgctgat agatgtgacg caatcctatc tgcaggtgct
     9721 ttccggagac gattttgaaa agggagacga cggcgatctc ggtgtgggag gccccgaaga
     9781 tgacgatcag gtgtcctcgt ctatccctca aatccaaatc cgttcccaaa gcggcgccta
     9841 cgggcgtagg cagcgtgtac acctgcgcga accccaaatc ttccagcagt tcgataccgg
     9901 cttgcacttg gaccggtgtc gacccgagag gagagttgaa gcacaccgag ggcttgctcc
     9961 agcccttcag taccgcgttc tttttcaaga atcgctcgaa aaacacgtgc gcgtgctcaa
    10021 attggtgtat gccgctttcg tcaaccgggt tctgaaaata gtagaacgga ggcgttttcc
    10081 ccaaatactc cttagcctcg gttccgaagg ctttcagctc tccgaattcg ttgtagagca
    10141 aaatcgtcgg ttcctcgacg aagaactcgg ttttgcctcc cacgatggaa tataggcgcg
    10201 tgtaatcagc gcctatatcc ataccggaaa gattcttcga cacccgacag cctcactaag
    10261 aatctaggaa atgcacgacc aagccgctct tcagtttggg ttcgaaccac gtcgatttcg
    10321 gcggcatagt ctttccctga tccgcgacgg cgaacaagtc ctcgaggctc gttgggaaca
    10381 tagaaaaggc taccttccag ttgcctgaat cgacgagcgt ttccagctct ttcaacccac
    10441 gaatgccgcc tacgaagtgt atccgtttat ccgttctcgg gtcttgaatc cctaagagcg
    10501 ggttcagtag gttttcttgc aggatagaaa cgtccaagcg ttttaccggg tcttcggcgg
    10561 ggaaagagcc cggtttcgcc cctaaaacgt accattggtt tcccagatac attccgaatt
    10621 cccctttctt gagcggtcta taggggcggt tcggcgcttt ttgaacggag aaattcgtct
    10681 ggatcgcctt gaggaaatcc tcctcgctgc gtccgttcag atctttcaca acccggttgt
    10741 agtctaagat cttcaactga ttgtccggga aggcaacggc cataaagaag ttgtactcct
    10801 cttctccggt atgcctagga ttcgccgctt tacgggattg ctgtgccctg ctggaagagg
    10861 cgctgcggtg atggccatcc gcgatgtaga gttcgggtag ctgttcgaat gcgcgttgaa
    10921 aagccgcgat ccgtttcggg tcgtttacga cgaacaactc gtggacgaca tcaagctcgt
    10981 cgctaaaacg gtactccggc tcggaccgaa gggtctcttg gacaagtgta tccaataggg
    11041 cttggtgttt ataagctaaa aagacgagcc ccgtttgcgc tttggtcgtt aaaatgtggt
    11101 taaggcggtc atcttcttta tctcttcggg tccattcgtg ttttttaata ctcccttttt
    11161 gatactcttc gcaagagaag gcgcccaaga cgcccgtttg cgtttgccct tccgggaaag
    11221 ggcatttcgg gcctttccag gtatggcggt agacaaaaag agccggttct cgatcgggga
    11281 cgaggtatcc tttctctatg aaggactcca ggtttgcccg agctttcgcg tagatctgag
    11341 gatcgtacag cgaaacggac ggatcgcaat ccacctccga tttcactacg tgcaaaaacg
    11401 agtaggggtt tccgacgacc tccgccctcg cttcttccga gtcgatcacg tcgtaaggtt
    11461 tcgaggcgat ttttcccacg aggtgtttag ggggtctgac cgctcgaaaa ggttttatta
    11521 ctgccatttg ttttcctcct tttatggttc tttcactaat cggtagctgc ctttttgacg
    11581 atccggttgg attttttttt ctgaaaccca cgttttcaat gtgttggtga cggtctggaa
    11641 aggaaagaat cggacgagaa agggttcttt aaaggtgtgt aactccgaga cgagccgcca
    11701 catctctgcc gcctccgttt ccggcttccc ccacaattct tcccggccgt actgctgtag
    11761 atcacggcac agtccggtat agtaggattt tagaaacacg tggtctttgg agtcgtattc
    11821 gaaggtttcc acgcgtgcgg cgcccaccgc ctcggcgtta aagagatggt atccggaaca
    11881 gagcggcgcg aacccaaaga actgcgattc cttgaccacc gcgatgatcc tcggtttctg
    11941 aaacagggta tagagcagga cctttcctcc taccaagcgg tacaggcgtg tgtcgggttc
    12001 tccttggaaa aacaggatct ctccatccgc gcacgcttta agatcgggtc cagcgggtgc
    12061 catcgttccc atccatcaat tttatcctca attttccaag ttcttgccga aggatatcgg
    12121 acatctcgta gtttttttcc gatcggtatc gatcccgtaa gcggaccagg aaatccacca
    12181 agagattaaa gttggaaagg atgtcttcgc gcctttcggc cgtgatctcg ccaggagatc
    12241 gagtccatcg ggcgccttcg gttccgtcca tcagcttgat tctcagtttt tccaattccc
    12301 ggcgaaggat atcggacatc tcttcgtttt tctccgatcg gtaccgatcc cgtaggcgga
    12361 ccaggacgtc catcagggta ttgaagtcgg agagggcgtc ttcgcgtttt tcaggggata
    12421 ccgtgtcgaa agcgtcttcg aatagtccga gtatgtcgcc aatatcgttc ttgatgagat
    12481 aataagcctg ccgcagccgg gattctccgt ccggttgcgc cagcgtctga ctgaccgggc
    12541 ccaactcttt gagcgtttca aagacgagcg cgacggcctt cggggtattg aaatcgtcac
    12601 ttaatgcctt catcaggtag gctcgttttt cctccagcca atcggaggct gctttgagag
    12661 tcgtatcgcc caaggtttcc tcgatttcgg tgaggaaggt tcgcactttc actgccgatc
    12721 ttttccattc gtccattcgt tcctcggaat agtccatcgg gcttcgataa tgtgtcgcga
    12781 acatatagag cttcaggcca tcggcgccgt atttgcggac cgcttccctg attttgatga
    12841 tattccccaa ggattttgcc atcttcgacc cgtccatctg gagcatgccg ttgtggagcc
    12901 agaggctggc gaaggttttt cccgttaggg cttcgctttg cgctttctca ttctcatgat
    12961 ggggaaagat caggtcgttt ccccccgcgt ggatgtcgaa agtgtcaccc agcaagacac
    13021 aagccataac ggaacattcg atatgccaac cgggcctgcc cggtccccaa gggctatccc
    13081 aggaaggctc accgggttta gcgtttttcc acaacgaaaa atcggcagga tggcgtttat
    13141 ccgggtcgac gtcgatccgg gctcccgcga gaaggtcctc gacttttcgg tgggaaagcg
    13201 cgccgtagtt cgggaaagaa ggcacgctga aaaagacctg agcgttggat acgtaagcgt
    13261 gtcctttttc tatcaaaccc tgcacgaacc ggatcgtttg tggcacataa tgagaggtgc
    13321 ggggggcgaa gttcggttct ctcactccca aggcctgcat atccttgcgg tactcgcgta
    13381 tatacgtcgc ggtgatggta tcgaagtccc atccttcgcg gttcatacgc tgtatgatct
    13441 tgtcatcgat atcggtgaag ttttggacga agaacacccg atatccccga tactcgaaaa
    13501 accgcctcat agcgtcgaag attattgctg ggcgcgcgtt gccgatgtgg accaaattat
    13561 agacggtggg gccgcacacg tacatccgta tctctccggg atggacggga atcaatgatt
    13621 ccttttgccc cgttagcgta ttggtcaaca cgatgggatg ggtttgatgc gtcgacatat
    13681 tacacctcca aacggcctaa tcggttaagc actttgttgc gccccagcag atggacaacg
    13741 tgcacgagct cggggcccgt gtctcgcgcc gtcaacgcca agcgaagcgt atggtaaaaa
    13801 ggtttcggat tcactttggt atctttgaca accgccttga tcaccgaaat gatttcattg
    13861 atctcccaag acgggatcga gtcgaagcgt tcatacaaga gtttgaaggc tgcagaaagg
    13921 cgatcgtcct ccaatgagcg taaagcttcg gcagtgggta tggggtccgt gaagaacgga
    13981 gacagcagtt cccccacctc gtccagttgg tgtattccat tttgtatcgc tttcatcgcc
    14041 tccagaagcc atttcttgtt gacgtcgacg ttttcgggag cgatgtaccc ggtctctatg
    14101 atgaatggaa cggccttttc caataaagct tccggggtca gttgtcggat atacataccg
    14161 ttcatccaaa ggagtttgcc cgagtcaaag atcgcaggat tcgagctgac ccgttccaaa
    14221 gaaaaggccc ggatcatctc atttaaagca cagatttcat tttcttgcgg gtgtgaccac
    14281 cctaaaagcg ccaggtagtt tataaggctt tccggcaggt aacccatctc tcggaagttt
    14341 tcgacggcgg tagcgccgtg gcgcttgctc aatcgcgaac catcgggccc caaaatcatc
    14401 gacacgtgcg cgaattctgg cagcggcatc tggtaggcct cgtagagcgc caattgacga
    14461 agggtgttcg tgaggtggtc gtctcctcgt atgacgtgtg tgatctccat cagggcgtcg
    14521 tcgacgacga cggcgaagtt gtagaccggt atcccggtac ttcggaagat cacgaaatcg
    14581 cccgtggttc cgcctttgaa aacgacgcgc cccttaatcc gatcgttcag gacgtactcc
    14641 ttctgaggca ttttgaagta gacgactggg ctccaccctt tttgcgcgaa ctcttgccgc
    14701 cgctcggggg tatcgtattg tgcgatggtc tcgtgggaat aacgagggga ctccttgttt
    14761 tcgagcatct tcccgcgcat cgcctccaac tcttcgggca aaacgtaggc ctcgtaagcc
    14821 ttgccttttt ccacgagcgc gtaggccatg cgtcggtaga tgtccaatct ttccgactga
    14881 cgatacggac ctttcggccc accgatatcg ggcccttcgt cccaatgaag gcccaaccat
    14941 ttcatcgttc gcagcaagga ctgttccgac tctagggtcg aacgctccag gtctgtgtct
    15001 tctatccgca gcacaaagac cccgttttgg tttttcgcga ataagtagtt gaataacgca
    15061 gttctggccc cgcctacgtg caggaatccg gtcggactcg gcgcaaagcg cacccttacc
    15121 atactttccc tccaaagaca cgattcttaa accttctcga gagttgtaga atgacggatt
    15181 ataacataaa aaactccctt tctcgagtcg agggtgggta taatatcggg gtacaccaaa
    15241 atgggaacgc agaacttaag ttcggcacgt ttttgctttt ctttaagccc tctagagtcc
    15301 ttaaacactt acgccacgat ccccgcgatc cgcgttttgt tttaaaagtt ttgcgatggg
    15361 tcaggaaaga agcgtatcgc gccgtcgcga aaagaagagg cgcttacgcc gcgataaccg
    15421 tcgggacccc gcgatccgcg ttttggtttt taaaagtttc gcgatgggtc aggaaaaaag
    15481 cgtatcgcgc cgtcgcgaaa agaaggggcg cttacgccgc gatggccgcg cagactcata
    15541 gcgcgtaaaa aggacaaaag aagaatctcc tcctctttct atatttgctg caactcgata
    15601 aaataaccac agcgagtcga cgcctcgagg ctttagaata gttttcgagc cgtattagcc
    15661 cgagtgggca aacagttcga aaaaacagat gaaaatctgg ctttctatga aaaaaacgag
    15721 aattctgata taatagtacg ggaaaatgga aacctctagg aagggacttc cgacgcgtag
    15781 atagaaaggg gaggggaaag atgaaaagag ttctgatggt gagtatactc gtgttcgttc
    15841 tggctgtttc gagtgtcttt gcgtttaacc tcggaaagca aaagtgctgg tacctcattc
    15901 aaccggccca acaggtgata ggggttaagg cctgtgttcc tgtttgtatc gatcctgggg
    15961 ttctcgaccc cggtttgtat caaaaactgg tggccttata ccaagaagcg gtagccaaag
    16021 gcgcgcacgc caacttttta agcgtccttc agatattgat agcgcagcac accaaccaac
    16081 agataggggt tgtgcccgcc gcgtcgcagt ctcagtattt ctatacgcat actttctcgg
    16141 acaaactgga caattgggcg ttttatcgcg ccggccagat acagagctgg cagcatgtga
    16201 cctccgggaa gacccgatac cttcaactca acgccacggg cagcgcgatg atccaagcgg
    16261 aaaaaatcat cccgatccca gcgtatatga aggggaaagc ggtcgtcgtc accgcgtctg
    16321 ttatggttga tctcaaagcc gccgcgttga actccccgac tgacttcgcc gctgccgggg
    16381 tcgtggcgca cctgtataac cgagactaca ccgcctactc ggaattacca ccttctcaag
    16441 cgaaggccgt tttgtggtcg acatccgagt acccaacgcg tcattcttcg ttcctctatc
    16501 cagatgaaga gatcattttc gcgagcgatc cttatcggac actgaccggt gtgaatatca
    16561 tcttttggcc ggacgtcacg gcgaatatgg aatgcgtcaa gatcggtttg atagcctttg
    16621 gggagggaga ttcggcttcg acgcaggcgg atatttggat ctccgacgta cgggtgatgc
    16681 tggcagacgc cgaatatcgc taacagaatc tgtataaaaa agaaggcgcc tgtgagcgcc
    16741 ttcttttttc tttgatcatc gacttacgcc ggtaccttgg cttctctatc aatcgtcttg
    16801 tgtagcgttg tttttcttct tcgacgggtt atttttcttg accaggggag ccgctttttt
    16861 cttgaccggt ttgttgatcg cgtgctcttg atgattcttc tgcgtgtgct cctcgtaagg
    16921 gagcgcgccg cgcttcgaaa tgcttcggat tccttcttcc tcgggttctt cgaattcgct
    16981 gaccgaatcc gcgacgtctt taaacaaaag ccggtggatt tttttcaaat tctcatactc
    17041 ttccggcatc gttttccaga actcccgccc gatcttgcaa agaaactcga gacacccgcc
    17101 gcagtttttt aaccctctgc ggatgttgca ctctcgtatc tcgcattcat cgctataggc
    17161 gaaagggcgg ttggacttac acccgaggca aatcatctga gaggctttgt aagtggtctg
    17221 gtgggttttg ttccactctc tcgccagctt ggccctttct tccggtttat cctcgacggt
    17281 ggcttgatag atcggacagg tgtggcagtt gatcccgcaa aagcctgtgc ctttcatgaa
    17341 aatagatcct cctgatttat aaaaatgtgg tgcgcgcggt taatcgctct cttcctcgtc
    17401 tccatcatcg acatcgagct ccccgacgag ttcgacccgg ggatctccga agtccttgat
    17461 ctcttcttcg aacgccgatt tgctcgacag ccgtttctct ttgcttggaa ggttccccat
    17521 cgatttgtct ctgagtagaa tgaaggtttc atatccctcg ggatcgaatc ttcgaaagag
    17581 acgagccatc ttttcggtca ggttgttggc cgtattcccg cgttgccaaa gatagttctt
    17641 gatatcggcc aggagttctt ttccgatctg attcatctgt tcgatttttt ctatcgcttg
    17701 caacacttgg gcgtcatcct tattccgtag catcctccga aggttgaaaa ggctttcggg
    17761 agtcgccggt tgttctcccg ctgcgttctt cgggtctatt tctctcgtgt ggtggagcgg
    17821 ttcttttttt gtcatttata tcctcctcgc tttaaccaaa aaagggcgcc cgatcccgcc
    17881 cttttttcga atacgctttt aaaccgaggt ctatttcggt tttggttccg gtttctttgg
    17941 tgtttctttt tttggttccg gttttccagg tttctttttg tctttctcgt cgggcatcgt
    18001 tttccctcct ttctgtttca accatctggg attgggtgat gcgaagacac gacaaatctg
    18061 gccatagtat actcctacgg aaaaaagatg caagcctttc cttcgcgcga aaacccttcc
    18121 taacctggct taaaacagag aacgaaagag aaaaggaaac gcaaaacctt ctcacgggtt
    18181 cgggttctgt ataaacattt caaagacggt ggcgcctgcg tagcctcatt tgcatcaaaa
    18241 aatcagcgct caatcgatca atctcaagag gtcgtccacg gtctctcttt ttcggatcag
    18301 gcgtgttttt tcgtcgttca ccaacacttc cgcggggcga aaggtcccgt tgtagttcga
    18361 agacattgaa tacccgtagg ctccagtgtc caagatggcg atgtgttctc ccggaatcag
    18421 ctcttcaagg gaccggttgt acgcgaggat gtcgccggtt tcacacaaag cgcccacgac
    18481 gtccacggtc atcttttggc gtttttccca tttctgaatc aaaggaacga ccatatgctc
    18541 cgcctggtac aacgcgggtc gaatcaggct ggccatattg gcgtcgcata ccaaaaatcg
    18601 tttgtagggc gtttgtttca aatacagtac cttagagagc agtaatccgg cggaagcgac
    18661 gatgtaccgt cccagttcca aaaagatcgg acaacggaac tgacgtagta acggtcctat
    18721 ctcctcacgg aaccgattca gatcgaaccc gcttccggcg tgcccgtaat cgatccccca
    18781 cccgcctccg aagttgacca tctgtatctg tgggttcctc tctttaaagg atagcagttt
    18841 ctccatcgct tccttgaaag gggctatttc ctggatgcta gaaccgatgt gcatatgaat
    18901 gcccgaaacg ggcagatgat cctcctgcaa gagcttttgc gcctcttcca tcggaatgcc
    18961 gaacttgttc tttttcagtc cggtggagat gtacgggtgg gttcgactgt ctacatcggg
    19021 gtttacacgt aagaaaaaac gcgtgcccct ttcgcccgta agcccgtttt caacgattaa
    19081 accgtactct tctttcgagt cgatgttgat cgcgaatacg ccatcattta accacgtttc
    19141 gatatcgagg agggttttgc catttccgtt gacgacgata cgctcgggtt tcaccccgct
    19201 gcgtagggcg atctggtatt cccccatcgt caccacatcc atccaaaggc ccttctgatg
    19261 aaagtagttc aggagcaacg gattgttgtt ggctttacag gcaaaaaata cggtagcggg
    19321 caagccctcc acgctctcca tcaccctcat aatggccgcg tccatctgtg cggcgctata
    19381 gacgtaggta ggggtgccgt atttctccgc aatcagatcg acgggcacct tttcgacgaa
    19441 ccagccgttt tcgtatcgta acatctgtag tccccctcgt gttgaaaaca aagataaaga
    19501 agctgtaaac accctgtccg atcaagtcgt ttcaggatga ggtataatca atggatagat
    19561 tataccaaaa aaggagaaga ttgcaagatg gccctcatca aacgcgtcac cttcaaattg
    19621 aacgaagtgt tctaccaaaa acctttccat atcaccaaca gcgtccgtag ttcatcgaaa
    19681 gtcatcgcta tcgccctcga gctggataac ggcataatcg ggaggggaga agcgtcccct
    19741 tccttccggg tcaacgggga gatcgagacc gtcttgatgg gctttgaaac gccgatctct
    19801 gaaatgttga gaaatagaga agtccagaga taccgcccgc ttttcgatac gttggacaag
    19861 ttttcgcgtt ccgcgcccag tctgaaggcg ggcgtgcaat acgccattct cgacgccttt
    19921 tcgacgctca tctcgactcc agtctatgag atactggggg gcgcaaaaga atacgtggag
    19981 acggatttta cgatcagcat cgacagcttg gaaaacacgg ttcaagacgc gaaaaaggcg
    20041 tccgaaaaag gctttaagat cctcaagctc aaggtcggag aggacttgcc ctccgacatc
    20101 gcccgtgtgt tagccgttca agaggcccta ccgtcgatgc attttgtcgt ggacgcgaat
    20161 atgggctaca cgccgaaaca agccgtcact ttcgctgaag cgatggtgag caacggcgtg
    20221 aaaatggacc ttttcgagca acccgtgtgg agcgacgatt ttgacgggct gcggttcgtc
    20281 cggcagcatt cgggtatccc catctgcgcg gatgaaagcg cgaagacccg cttcgacgct
    20341 ttgaggttga tccgagaaga gtgtgtcgat tatatcaaca taaagcttat gaagtcagga
    20401 atatcagatg ccttggcgat cgtcgaactc gccagaagcg cccatgtcgg cctgatgatc
    20461 ggctgtatga gcgaatccgg agtaggaatc gcccagagcg ttcatttcgc ttgcgggacg
    20521 ggagcgttca cacaccacga tctcgatagc ccctttttgc tcaaagaaac caccccgtat
    20581 aagtaccgga cggaaggctg tcgccttctc ccgatcggtt agtagacttc gcccaagcga
    20641 ccgaagagag ttcgatcgtg gatgtgatcc tagagtcctg cgcttcgtac gaagaggctg
    20701 aaaggcagct tcctcagcat cttaagcgat acgaagggct gttttggaaa aacgaaaagg
    20761 tgtgggtgaa gcccaatatg ctgtccgcgc gaagcccaga ggagggggtt acgacgcatc
    20821 cgaccatcgt tcgaggggtt ctacggttcc tcttggacct gggtgtgaag ccggttgtgt
    20881 ccgacagccc gggggtcgga aacgtaagaa aagtggccgt ggcctgtggc atcagccaag
    20941 tgtgtcagga gttggccgtg ccgctcatcg agatggaaga gttcgtggag aaggacggag
    21001 acgtcttcaa gcagcttcgc ttgggggatc tgatcgatcg ggtggatgcg gtggtcaacc
    21061 tgcccaagtt gaaaacacac ggccaaatgg tgctgacgat gggggtgaag aacaccttcg
    21121 gttgcgtgtt gggattgaac aagcccgcgt accatctgcg cgcgaaagac tatcatctgt
    21181 tcgcggattt gctcgtcgat gtgcatcaat tggtgaaacc ggttttaacg gtcatggacg
    21241 ggatcgacgg gatggagggg aacggtccgt caaacgggca aaaaaaacgg ttgggtcttc
    21301 tcgcctcttg caaaaatgcc tatgccttag accacgctgt cgcgatgcgc ctacacgtct
    21361 cgccggacaa agtctatacg ctggcccgcg cgaaagcccg agggctgatc ccggagtaca
    21421 agattcacgg aaattggagg aattcggtca agatgccaga cacggtagga ttgacggaga
    21481 tcgttaagat gccgctttgg ctacaggaac tcacccacca ctgggctcga gtgccggtat
    21541 tcaatacccc gaagtgcatt caatgtaaaa tatgcgaaag gcactgtccc gcgaatgcga
    21601 tcacagtcga caagcaacac atcgattata aaaaatgcat acggtgttac gtgtgtcatg
    21661 agctgtgtcc cgaagatgcc cttatgctaa aacgaagact ctggaaagct ggtggaagat
    21721 gaaagacaag cgactcttga gaactctgtt ctggaccttt ttcaaaattg ggtgttttac
    21781 cttcggcgga ggatacgcga tgatcccttt gatcgaacga gaggttgtcg atacccatca
    21841 ttgggtttca cgcgaagaaa tcctggatat tttagcggtc gcgcaatccc tccccggagc
    21901 gatcgcgatc aattcctcaa ccctgatagg ttataaaatg gcgggaaaga ctggcgcgct
    21961 gatctctact ttaggaatgg tgctcccctc ttttttgatc atcttgatca tcgcgatggt
    22021 gttttccgcc tttcaggaga acgagtgggt gcgtaccgct ttcgtgggga ttcgcgccgc
    22081 ggttgtaggg ctcatcgtga tcgccggtta caatatgttc aaagcggcgg tcaaagataa
    22141 gatcgggata gggattttgg tggtcacgtt gtttctttta ttcctactcg acgtccaccc
    22201 gatccttttg cttatcggcg gagcgggttt tggaatcctt ttgtacatcc tctttccaga
    22261 gaaagggagg ggctcgcgat gatcatcgag ctattctcta ttttctttaa gatcgggttg
    22321 ttcagctttg gcggtgggta cgccatgatt ccaatgatac gaaacgagat cgtctctcgg
    22381 ggttggttga cggcagaagg cttcgccaac atcgtcggta tcgctgaaat gacgccggga
    22441 ccgatcgcga tcaatatggc aaccttcgtc gggtttaaaa cgatcggttt cttcggcagc
    22501 gtggccgcca ccttaggtgt agtgatgcct tctgttctcc tgatcctgtt gatctcgaag
    22561 atattcttca aataccagag tcatccgatc aaacaggggc ttttttacgg gatgcggccg
    22621 gtcatcgtcg ggttgatact atcggccgcc tacttcatgg cggaaaccag cctattcatc
    22681 gtgaaacccg gagaatggac gggaaatttc ctatcgcagg tcgattgggt gagcgtggga
    22741 atcgccctac tcaccgtcgt tggcattcgt aaatacaaac ttcatccgat cctattgctc
    22801 ggagtaggag gagccattcg catcgggctc aaattcgttg gtgtatgaca ctaccgtcgt
    22861 tgcgcctttg cgccgactct ccttttttac ctacccgtct ccttggtttt tttggatagt
    22921 attttttgaa agcttatcgc gtgttcctca ccaccggata agactacgat ttttctttca
    22981 atggcaggag atacgtcgtg atgaggtgat ggattgtctg cgcggttagc ggcttcgtaa
    23041 cgaacccgtt cattccgctc tcaagcgccg gggcttcgac ctcttgatct atgaaggcgg
    23101 acagggcgat gatcgggagg tctttgaacc gttcctctcg ccggatgagc ttcgttgtgt
    23161 ctataccatt caggatcggc atctggatgt ccataagcag taggtcgtac cggttctgtt
    23221 gaagcatttc caaagctttc tgcccatttt ccgctaagtc gaccgatatc ccgtggcgtt
    23281 gaaagatacg tttcatcaga ttctgtgtga tttcgttgtc ttcggcgatg agtatatgga
    23341 cgccctctaa atcgtaagtt acgggacctc cgttttcgct cttctttgtg taagcctctc
    23401 cgtcaactgt catcgggatc tctatgacca cggtcgttcc cttccctgtg gaagactcca
    23461 agcgtaaaaa ccccttcatt cggtctacca gttctttggt gatcgaaagt ccgagacctg
    23521 ttccaccata ttttttggtc agataggatt cagcctgttc aaacttgtca aatatgcgct
    23581 ggaagtcttt ctcttcgatc ccaatcccgg tgtcagcgat cttaaactta aaaagcattc
    23641 gatgctcgct gttttcgata ggggtggcgg ccatttcgaa ggttacggcg cccttttctg
    23701 tgaattttat cgcgttactt aacacgttat tcaggatttg ctccaggcgg agttcgtcac
    23761 cttgaacaat aacgggcatt tcgggctcac gtttcacaaa gaagtcaagc gctttggctc
    23821 ttgccaggtg cgagaagttg tcgacgatac ggtcgagcat ttcaccaatc atgaaatctt
    23881 cttcgcgaat acgtatcttc ccatcttcga ttttcgataa atctaagatt ttattcacca
    23941 ggttcaacag atagcggttg ctagcatcga tctgttcgag catcttcctc ttttcgggat
    24001 tctcttctgt ttccaaaagc aggtgtgtga aacccataga ggcgttcatc ggcgttcgga
    24061 tctcgtggct catattggat aggaaggcgc ttttggcctt caacgccgcc tgcgcctctt
    24121 ctttcgactt gatcagcgct tcttctaatg cttttttcgc tgtgatgtcg atgtttatgc
    24181 cgcgataacc gactgaccga tgccgttcat cgtaagccgg gaaccatact gacaagaccc
    24241 accgcttacg cccatccctg gtacttactg gatgttcgaa attccagagg gtcttttccg
    24301 aacgcatcac ttccgttata taagccttaa actctcctcg ggtttcttca ggatgaaggt
    24361 cgtacacact catcttgccg agcatttcct cttcggtaaa tccgttgacc tcgggattga
    24421 ggttccgaag gcttacaaaa gttccgttta ggtcaacctg ccaaaccact gcgccagtct
    24481 gctgaagcag ctcttcaaac tctgctttga ttctctcaag agccgcattt ttcgattcga
    24541 tctctataaa agacctctcc aacgtttggt tcacggcgtt cagctcttcg ttagtcgcga
    24601 tcaactcttc gttcgtggct g
//
LOCUS       DAOPXN010000008        24200 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2059_length_24200_cov_5.224601, whole genome shotgun sequence.
ACCESSION   DAOPXN010000008 DAOPXN010000000
VERSION     DAOPXN010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 24200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24200
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_24200_cov_5.224601"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PKM49_00955"
     CDS             <1..334
                     /locus_tag="PKM49_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81413.1"
                     /translation="TKMHFSRALSEKLFIYEIDAGANRLYVGPYDFLMKETATISDTT
                     WYVPLEDREYRCVIRKKAKEELTRVTSLGGDRYRLSFAHEVFAVTPGQTAALYDEEGI
                     LIGSGVLV"
     gene            complement(397..4242)
                     /locus_tag="PKM49_00960"
     CDS             complement(397..4242)
                     /locus_tag="PKM49_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY81414.1"
                     /translation="MSRNSEKIGRNRQTHQRIALKNIILIIVLLVGILALGLFFCERG
                     LSKWIDGEKRSVSEISDLKLQRLVDWSEARLKTRGLLFQGAALSNVLSEWLLKPNVPP
                     SASLVDRLEAYSSARDIAALFILNSNKELLFNTNDDTIVWGEDTLKALDYCVARQEAQ
                     LSDFFVSDTGKVCQDHILPIFNDEDTDRQLYGFQILRVELTSLSPYLLRLEQERASLS
                     LYFIARTDDGFRVIQLNREGGSVVNGEGTVYGDLRLEDIESALASDYTLVRKDRHGDI
                     AFWSAKRIPDSEWYFFSKVTSYDFRNRVRRTYLPFLLHWGFVCVIVAALFIFINQKRE
                     TERLKQLEEQRRKADLLRLPRELMMKHANECYILTDDRFRILDANDVALSRYGYETKS
                     MLGAPLDLLFTSRSEGTEIHPRLAGAQNGESVDALYVSKEGGISPVELNVRSIEVQGE
                     RYYAITAHDTLERKAAERLLRESEERFRSFFNNLAAGVVIHELAVDENGRPIDFSIVD
                     INPQYERWMNVKKEDVVGKRVTQAFNIEKPPHLDAYLLEGNKGPTRPIIQYIPVANRT
                     FEIYAIPLGKGGLATIFVDVTEREKAEKALKDFQAELSETNLKLRQSIKNAKEMATRA
                     QAANVAKGSFLANLSHELRTPLNGVIGITGLLMETALTPEQQEYAQIIRSSGEVLLTL
                     INQVLDYSKIEAEKLNLEIIPFNLRRLIEDTLDYLSFPAQKKNLEILVSIDSQVPDAL
                     EGDPGRLRQILSNLVDNAVKFTPFGEIWVRVECDEETDVDITLKFSIKDSGIGISKAQ
                     RELLFSPFVQLDSSTTRKYGGTGLGLAIAKHLTEMMDGKIGVDSDIGKGSTFWFTARF
                     KKAEETVGSIRPSIPIFANVSAIVVDHNRSSRSHLKNLLERFKLRCFEAENGEEALEK
                     MRKEYETGREIDLVLFASSMSPDDLQLVRRIKSQVEWRRTKVVLMRARKAEESLERGE
                     AAEFDSILTKPIFESQLVDLFRGVLGEETEELTTLEKEFSSDVKLERATIRNRYKVLI
                     VEDNITNQVVAMRMCEKLGFRVEAVANGREALEVLNTVSFNLVLMDCLMPVMDGFEAT
                     RKIRGGYTGEANTRIPIIAMTAYALKGDREKCLDAGMDAYISKPVQLWELAKVMDEVL
                     GLEDLPIARESLKQEVGNVPPPVIDSADFAERLGGDPEAAQELISVYMTDIPKQLAAL
                     REALEQKAYDPAIRLSHRIHGASLNVSANPMSAAAKDLENALRENRLDETAGLLDRME
                     KALEQLRFHFDAGNQEEST"
     gene            complement(4336..5595)
                     /locus_tag="PKM49_00965"
     CDS             complement(4336..5595)
                     /locus_tag="PKM49_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81415.1"
                     /translation="MRKLVLGICCAVLLVSFAFAAPVTVEFWHAMGGGHGETLAEIVK
                     LFNQQNADMQINAVYIGNYNALQQKLLASVQSNTLPGLSQAYSNWTSKLLHSNIVQDL
                     TPFLNDPEVGLTKEEWEDIWEPFQKMCTWGDKVYAIPFNKSIYVFYYNTDVFDMEGLD
                     APKTMEDLYAICRMLTVDENNDGTIDRYAFGYRTTVDHFGVFLYAFGGEIVHENEDGT
                     YVVKINSQETRKTLTFMKRLKDENIALVQGGYLDAPFGEGSVASFIETIASKPYVESG
                     SKGKHDWNWTSVPFDVKLNPPFAGTDVIMFNTIPQEQKKVAWEFLKFLCSPKVTTFWS
                     LKTGYVPVRKSALETSEWKIFVKMDPKAAIPVKFIGQGYSDPKPSTWEEIRGTVTNML
                     NNVLFDKWTIEEGLAWAEREIKNYLAK"
     gene            complement(5664..6623)
                     /locus_tag="PKM49_00970"
     CDS             complement(5664..6623)
                     /locus_tag="PKM49_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81416.1"
                     /translation="MDWLRALLDTITNPVFYRLTLSSATPLLFAALGGLFSEKTGVTN
                     IALEGIMLLGAFFGILGSAFTGNPWIGVLLAMVAGLGLASLHAWASIRFCADQIVSAT
                     ALILLAQGLTGFLLEPIFGQPGSTHIFNRVPEVRLTFLGKDTFLYKMIGTLSPFVYIA
                     FALVIVTWILINKTRLGLRMRAVGENPEAADTLGINVFKIRYFGVMMSGILAGLGGAF
                     LSVGELGQFSENMTGGKGFIALAALILGNWSPVGAMWASLLFGVAQALNEQLQSNPIL
                     SIPSTTKPLFNLLPFVITLIVVGGFVGRTRSPAASGTPYEKRG"
     gene            complement(6598..7728)
                     /locus_tag="PKM49_00975"
     CDS             complement(6598..7728)
                     /locus_tag="PKM49_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81417.1"
                     /translation="MSKGWQRIFIPLMAVVIALAIASVIILFIAEVPGPSTGNLWNDF
                     WNQIGVRFSFLGRAFQAMFEGAFGSQFAWMNTVIRMTPLILTGLAVGFGFRAGVFNIG
                     AEGQMAMGALMATIVAVNIPGLPMFVAIPVCMLTGMLAGGVWAGIAGFLKAKTGAHEV
                     ISTIMLNWIASNITNWAVTAPFATGSGTPKSPEIAVGAKLPILFSIEATFLSYGIIVA
                     VLVAFVMYIILYKTSMGYEVKAVGFNPYAAEYGGIQVSKNLIMTMAISGALAGLAGTL
                     ESMGLYYRFYGTLTSGKGFDGISIALIGQNHPLGIIFASMLISGLRTGSNQMQFIGVP
                     KHIVTIIQSIIIFLVAAERMVRTIMGWDRKKKVKDGLAKSAS"
     gene            complement(7741..9348)
                     /locus_tag="PKM49_00980"
     CDS             complement(7741..9348)
                     /locus_tag="PKM49_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81418.1"
                     /translation="MPFHIPEPSEYPHYAVVMKGIVKQFPTVLANDHVDLYVRAGEIH
                     AIVGENGAGKSTLMNLLYGLYTPDAGKIHINGKETLIKGPAEAIKAGIGMVHQHFMLV
                     GPLTIAENIVLGEEPLDSWRFFDLKAAKKRVRELSETYGLHVDVDMRIEDIPVGTQQR
                     VEILKTLYRGANILILDEPTAVLTPQEADELFVVLRNLTDKGKTIIFITHKLNEVMAI
                     SDNVTVMRLGKVTGRIPISETSPRQLANMMVGREVLLRVEKAEKKAGEAVLAVNGLHV
                     KDNRGLEAVKDLSFTVHQGEILGIAGVAGNGQTELIEAIAGLRKKEKGKIELCGKDIT
                     HSSPRDIRELGLSHIPEDRYKHGLVKEFPVYYNILLGRHYLPPFSKNGFLRHKQVYER
                     AKFLIKQFDVRPPSILMLAENLSGGNQQKMVVGREIDYQPRCMLVAQPTRGLDIGAIE
                     YIHRTLIGLRDAGVGLLLVSMELEEIFSLSDRILIMFEGEFMGEAKPGEITLEEIGLA
                     MAGTPLAEVRKTEAASAGKSETTRVES"
     gene            complement(9423..10418)
                     /locus_tag="PKM49_00985"
     CDS             complement(9423..10418)
                     /locus_tag="PKM49_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY81419.1"
                     /translation="MKRLFVLALVLLVSTVFFGNFIVTMVTDTGGLGDQSFNDGTWSG
                     IKAAEKELGITAKVVQSYEQADYVPNLSNAAKESRVVVAVGFMMEDALMQVAPQYPNV
                     AFVGIDIGDMGIPNVKTYTFKEQECAFLAGFLAAAMTKTGVVGYIGGIPIPPVQRFEW
                     GYRSGIHMWNKLFDDDVTVLIGYTQSFTDSQKGKTMTEAQYSQGADIVFAAAGLCGLG
                     TFEAAKSAGEGYYAIGVDVDQDHLAPGRILTSATKGVDMASYYAVKDAYEGFFEGGHE
                     NLGFIEGGARLSEMKYTRNIIPKWILVMLKGLEAQAIAGDFVIPDSEAAVEAFLK"
     gene            complement(10415..12133)
                     /locus_tag="PKM49_00990"
     CDS             complement(10415..12133)
                     /locus_tag="PKM49_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81420.1"
                     /translation="MKKLSLYIFFLSFFVIAWGISFDESLERFEGWIALPLDQSNQIF
                     EAINDISVYRKYRLAMIGPIRDDERFYDVNKLLELVFDSIKTGEINEDLSLSAYLAYL
                     SAHLFNRDLTVGGLNSFAPYYTTYNQANNHLRDTAIQYFSHWIAHGVGIVAAPPDARF
                     SIDPPAKALKTRLRYAFTPDETLFPLIVSGFTSETEENLKQAIARVKAETAKTISDQD
                     LDRLIKRQAQTVGAPIYTTLLPDQTKLAKDFVDRAPKAVNLWAYRFLAYGLVLLAVIF
                     LKKWRALILTLLILFEAFVQYHTQGYLFSDSEAFVYGLLTFSLLGFSLLVWLSRVSNK
                     KRRGAYEWTHLWMYLAIGALWFFPYWVAPEALRMDRQETFYQSAVYEVLKEELFEWKR
                     GLYEEAFNEYLISREATPRFTEILRGRLNKAMPFSGIKLREETESYLLSKWKSARFLP
                     LETSVMQAYSEASRSDFRSPNFYLFQSVEGSFALLLITLATATLVLLRSKRRVVLAIY
                     ACSWIPFFAMLFRSDIHLLVEKGYPVLPLTENHPGMFVLVLAITCFLFSIYLSWKTYQ
                     KEGKKQ"
     gene            complement(12240..12872)
                     /locus_tag="PKM49_00995"
     CDS             complement(12240..12872)
                     /locus_tag="PKM49_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81421.1"
                     /translation="MLALHLFLAHTVADYSFTNPMKLYGEGSSWAILKHAAWFAVVFL
                     AFTFDTVFSSGYGITLFFGSLVLHGLIDCLRFKNKKVWWVETVSWLSFLAIGIFSSVF
                     FTGSYITPAFAMYLVGMVSVSVIPTQIFRMIGWIPKMENESDGISERLAIFIFLLALN
                     WPLALASIGCGLSYRLIFRKMTPPLWWVSPTLGIAVSLLFRWVIYRSFSF"
     gene            complement(12876..13775)
                     /locus_tag="PKM49_01000"
     CDS             complement(12876..13775)
                     /locus_tag="PKM49_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HNY81422.1"
                     /translation="MNLLQVEESMAAGGQAVIEGVLMRAKKTAIAVRQPDRKIVTREY
                     DKPMLIKRFPVLGTPFIRGVCVLWDSMYMGIVALSWSAEVSSQDTGEELKKKDIVGAI
                     LFALALAIGLFVLAPFLVAQLIPAIKEDPVSFALFEGIIRVAILVLYIWAVSRSKDVK
                     RVFEYHGAEHKTVHAYEAGVELTVNEVRKYSRIHPRCGTSFLFITMIAAIIVLIIANS
                     IWQVGFWQKLLIRILFIPVIASISYEFLRFTAKHLDNPLFRALAWPGMLLQRLTTAEP
                     DDDQLEVAIVSLKSAVGALETEV"
     gene            complement(13756..14028)
                     /locus_tag="PKM49_01005"
     CDS             complement(13756..14028)
                     /locus_tag="PKM49_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81423.1"
                     /translation="MRDFYTPSEEELNQIAIACSEDERLREILGRILSFSEEERESFR
                     QKMTLFFIGAETDEDIKAKRVFSVLIERVYAEKIGKKVALNELTTS"
     gene            complement(14025..14306)
                     /locus_tag="PKM49_01010"
     CDS             complement(14025..14306)
                     /locus_tag="PKM49_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HNY81424.1"
                     /translation="MEVLKVSSNSNPNKVAGAIAGELSKNQCVELQAIGAGAVNQSVK
                     AVAIARRFLDEQQKDLYMVPSFEDVNIDGETRTAISVKVTAVEKPKVDE"
     gene            complement(14430..14891)
                     /locus_tag="PKM49_01015"
     CDS             complement(14430..14891)
                     /locus_tag="PKM49_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81425.1"
                     /translation="MKKRGLGVLIAVLALVFVISSCVLFKGTPVGKKWKGTVNLTTAA
                     GQVAAGPYNVSITLEFEWLFGNSKIIGGQVTLTPALAAGKRAGAEPYKFNITGGEYAE
                     ADTTLSFKAKEVDTKDILDFTGKVVEKAISNGVITFGVLTVGSFAISEQTS"
     gene            complement(15056..15445)
                     /locus_tag="PKM49_01020"
     CDS             complement(15056..15445)
                     /locus_tag="PKM49_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81426.1"
                     /translation="MDRCDYCGTDEQVTVYALDSEYYGKTVRLCPSCLRNKITTSFRP
                     IDKLKLDDMIRSFLLVNDSPYSNAELKRDENCSFFLYPYLIGFELFEREVEAPVFESW
                     SREVYRRWVHFLTTVQRLENKRRKKGP"
     gene            complement(15432..16064)
                     /gene="pth"
                     /locus_tag="PKM49_01025"
     CDS             complement(15432..16064)
                     /gene="pth"
                     /locus_tag="PKM49_01025"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNY81427.1"
                     /translation="MTESDNTEGDTEMVFFGLGNPGTEYHDSKHNLGFQVIDHFVTTH
                     RKEILESRFLPTLYQYWSCRNRFGQAYLLIKPLTFMNRSGEAYRHAQGHFGYSDGESV
                     IIYDDVALPLGAVRIRAKGSDGGHKGIRSILSVTGSDEIARIRIGIGPKPPAMDLSDY
                     VLRPLKITERSIINETIDTVADAIERLTYERVETVMNQINRFSREKYGSL"
     gene            complement(16069..16713)
                     /locus_tag="PKM49_01030"
     CDS             complement(16069..16713)
                     /locus_tag="PKM49_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNY81428.1"
                     /translation="MAKQALQVAERTRLKTSGSRKLRARKVLPGTAYGPDFGPLNIEV
                     GREEFMIMIQKVSTTTSIDLKIAKSDGSSMVKRAYLKQVQRHKVTDFPIHFDFFVPSE
                     GHVMRIEVPIEYQNTPKGVDQGGRVDTFHESVEVEALPKDIPEMVSVDIANLDLGDFL
                     KVKDLHLPPGVKPLLDPEEILLAVTMPKEEVEEEPAGEEEVTQPEVIREKKEEK"
     gene            complement(16755..17705)
                     /locus_tag="PKM49_01035"
     CDS             complement(16755..17705)
                     /locus_tag="PKM49_01035"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNY81429.1"
                     /translation="MPYNRPEMKVFAGSSNPELAQKMADYMSVKLGEIDIGKFADGEV
                     NIKIGETVRGHDVFIIQSTCTPVNDNLMELLVMIDAFCRASANSVSIIMPYYGYARQD
                     RKARGRDPISAKLVANLITVAGAKRVVTVDLHADQIQGFFDIPLDNLRSFPVFCNYFT
                     EEDSFNPEDYVTVSPDVGGVKRVRRFAEKMKTSLAILDKRRPRDNVAEIVNVVGDVKG
                     KKVILFDDMIDTGRSLVGAAETLLNVGAVSVQACAAHGLLSGNAAELLQNSLIDRILL
                     TNTIPHRSLPDKFKILDISHLLGEAVIRVRKNLSVSILFR"
     gene            complement(17754..19166)
                     /gene="glmU"
                     /locus_tag="PKM49_01040"
     CDS             complement(17754..19166)
                     /gene="glmU"
                     /locus_tag="PKM49_01040"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNY81430.1"
                     /translation="MLFIVLAAGFGKRMKSNRPKVLHKLVGKPILGWVLCACQKAFDP
                     GQPNRLAVVIHPDMQEAVDYIEGWKKEAGLENVEICFQRERLGSGHAVLMAKEAIDRC
                     EKGESVAILSGDVPFVRPETVRELALCAKELSGAVLTVDNPNPHGYGRIFRDPQGHVE
                     RIVEEKDLSPEQRSIREVNAGVYVFEARALSWALDRLDNHNAQGEYYLPQTVEILVRN
                     RGKVAALTINDPVEVSGVNDRCQLVTLDRLMRLRINHRWLAEGVTVIDPENTTIESDV
                     QIGMDTIIQPYCFIAGKTVIGKDCVIGPFSQITDCSIGDGCEIDRSHMRGAKVSKRVR
                     IGPFARIREGTELSEGVSIGDFVELKKTSMGVGSKAHHLSYLGDATIGENVNIGAGTI
                     TCNYDGYHKYPTQIGNNAFIGSNTALVAPVSIGEGAIVGAGSVIVENVEKDALALARG
                     QQIQKPGRAAVIRERNKKRP"
     gene            complement(19290..20720)
                     /locus_tag="PKM49_01045"
     CDS             complement(19290..20720)
                     /locus_tag="PKM49_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY81431.1"
                     /translation="MRLFNIFKQDLSAQEVAEVRREVLAIALPAMGENALQMVLGLVD
                     TAFLGYLNWQAMAAAGLANQVFFIFQAFLVAIATGSSVLVANAVGAKNQNAIRSATWN
                     AIYLALILGLVITCFSLISSGLVFLGGAGASAEVRGMAASYLQINLLGGVVLSLMFVL
                     SALLRGSGDTRSPLYASILANSLNALLDYILIFGKMGMPKMGIEGAALATVISRAVGL
                     VILGVILFRSPRFQLRVKENRRFSSKRTKEILRVGLPTSMENFFFSLGMLVFANILLI
                     AGDQAYAAHRVGINIESISFMPGFGVSVALTALAGQYNGRDDLRMVRGIVKQGWIIAC
                     AFMVCAGIFISLFPEFLIRIFTADKDIIDMAAVPVRIIGLVQFFLATDSAITGSLRGV
                     GETKLPFFISLTAVWGLRLPIGFILVKFFNLGLLGAWIGMMTDFITRSILKLTVYASG
                     RWIKTAEKTRRAVGIEKERRLTDQTT"
     gene            complement(21437..22675)
                     /gene="mutT"
                     /locus_tag="PKM49_01050"
     CDS             complement(21437..22675)
                     /gene="mutT"
                     /locus_tag="PKM49_01050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HNY81432.1"
                     /translation="MGYYARAKNLRRTCQIIVERYGGRIPEDYAALLKLPGIGAYSAG
                     ALLSIIYERPFPSVDGNVHRVFSRAYALDDVANARDEKRRTHNLARDYIQIAFQGGLS
                     PSDFNQAYIELGALVCSPKKPLCDKCPIAGDCVALQAGLQRVRPLKEKSRALETKEME
                     VGIIWSNQRLMLERRDETGLLKGFWGLPTTEAFLGAEPGQSLVRYLATRYDLETIYPR
                     ELFVEQHVFTHQRWRMRVFHAFCELSCSDEHDPERSFAFFDSREIEALAIPKAFRKVL
                     ARYAERPLHVVAAIIKDETGRVFTAKRKPGGYFGDYWEFPGGKVEPGETEEAALKREI
                     QEELSIVVEPTRFLQRNGYSYPFGQLQISFYECERKGGTIRLTEHREGRWLKPEQMKP
                     QEFPPADAMIIEALQRALGK"
     gene            complement(22682..22849)
                     /locus_tag="PKM49_01055"
     CDS             complement(22682..22849)
                     /locus_tag="PKM49_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81433.1"
                     /translation="MPWRRTNDPYAILVSELMLQQTGVETAIRYYEPFLLRFPTARAL
                     ARAETEEVLKS"
     gene            complement(22949..>24200)
                     /locus_tag="PKM49_01060"
     CDS             complement(22949..>24200)
                     /locus_tag="PKM49_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81434.1"
                     /translation="ALCLIFSLCVLGFSDNLLGLRTVEATTTEAATEKTETGKLSLKT
                     IDPLRASFQAGWKAPSLPGWKEAVVGGYTLKLPAGFSLNASKSGSNFDAEIVDSSGQL
                     FARLFVYQLDAYKLDELLVDLTTSLYGVRTSGSKSFEEYSDLQNGRTAYLTKLTLSDQ
                     LFALPVLLLYQKGAQQTDVKSGTVVMLIVEPGLYQAPEKQDLLAAWIEGIGGSLIGSY
                     APASSAKTTEPVTIPVKETPKTTGLPAGKDFGALLIQRLSAEEWLDSPKRGWKTAYGD
                     SFYVFYPAELKPDYFYADQLEGFDFVSTDMTVAKLFVGYIDEELLCDELYQDLLSSYL
                     GGLGNYNLIKKTPYLDLQLGFVKTYELSFSDMRAWIMLFSESIIEDVFQGEYIVFIGI
                     APTRETETWKDIYLDMIMSVSFLN"
BASE COUNT         5672 a         6672 c         5963 g         5893 t
ORIGIN      
        1 aacgaagatg catttctctc gcgctctgtc cgaaaaactc ttcatatatg agatcgatgc
       61 cggtgcaaac cgtctttacg tcggccccta cgatttttta atgaaagaaa ccgcgacgat
      121 ctccgataca acttggtacg tgccactaga ggatcgggag taccggtgtg tgatccgaaa
      181 aaaagccaaa gaggaattga cccgggttac gagccttggg ggcgaccgct atcgcttgtc
      241 cttcgcacac gaggtcttcg ccgtcactcc cggacaaacg gccgccctct acgatgagga
      301 gggtatactc atcggttccg gtgtgctcgt atgacaggtc tggtgttcta tgcgtaaaga
      361 agaatgacga cctgaaaccc aacgcttcaa aaagagctac gttgattcct cttggttccc
      421 agcgtcaaaa tggaatcgaa gttgttcgag ggctttttcc atcctgtcga ggagtccggc
      481 ggtctcgtcc agcctgtttt cacgcaacgc gttttccagg tccttcgccg cagcactcat
      541 cggatttgcg ctgacgttca ggctggcccc gtgaatccgg tgggataagc gtatcgcggg
      601 gtcatacgct ttttgttcca aagcctctcg gagcgcggcg agttgtttcg ggatatcggt
      661 catatacaca gagatcaatt cttgagcggc ttcgggatcc ccgcctaacc gctccgcgaa
      721 gtccgctgaa tcaatgacgg gtggggggac atttccgact tcttgcttca gagattcgcg
      781 ggcgatcggc aagtcttcca gacccagaac ctcgtccatc accttcgcca actcccataa
      841 ttgaacgggt ttagaaatat acgcgtccat acccgcgtcg agacacttct cccgatcgcc
      901 ttttagcgcg tatgccgtca tcgcgatgat cggaattctc gtgttggcct ctccggtata
      961 gccgcctcgt atcttacgag tcgcttcaaa accgtccatc accggcatca aacaatccat
     1021 aaggacgaga ttgaaagaga cggtgttcaa gacctccagc gcctctcttc cgtttgcaac
     1081 cgcttctact ctaaaaccca gcttttcaca catacgcatt gcgaccacct gattcgtgat
     1141 gttatcctcc acgatcaaca ctttgtatct gttccgaatc gtcgctcttt ccagtttgac
     1201 gtcggaagaa aactcttttt ccagcgtcgt cagctcttca gtctcttctc ccaagacgcc
     1261 gcggaagagg tcgacgagtt gtgattcgaa gatgggcttg gttagaatac tatcaaattc
     1321 cgcagcttct cccctctcta aggattcttc cgcttttctg gctcgcatca gaacgacttt
     1381 tgtacgtcgc cattcgactt gggacttgat ccgcctaacc aattgcaggt catcaggaga
     1441 cattgaagag gcgaacaaca ctaaatcgat ttcacggccc gtttcatatt ctttccgcat
     1501 tttctccagg gcttcctccc cgttctccgc ttcgaagcaa cggagtttga acctctccaa
     1561 taggttcttc agatgacttc gactacttcg gttatgatcc acaactattg ccgaaacatt
     1621 tgcgaagatc ggtatcgaag gcctaatcga accaacggtc tcttcggctt tcttgaaacg
     1681 cgccgtgaac cagaaggtgg atccctttcc aatatcggag tcgacgccga tctttccgtc
     1741 catcatctcg gttaagtgtt tggctattgc cagtcctaga cccgttcctc cgtacttccg
     1801 ggtcgtcgaa ctgtccaact ggacaaaagg agaaaacaac aattctcgct gagccttaga
     1861 aatccctatc cccgaatctt taatgctgaa cttgagggtg atatcgacgt cagtttcttc
     1921 gtcacattcg acgcgaaccc atatttcccc gaacggggtg aatttcaccg cgttgtccac
     1981 gaggttcgaa aggatctgcc gtaagcgccc cggatcccct tccaatgcgt cgggtacttg
     2041 ggagtcaata gagaccaata tctccaaatt tttcttttga gcggggaacg agaggtagtc
     2101 cagcgtgtct tcgatcagtc gccgaaggtt gaaagggatt atttcgaggt tgagtttttc
     2161 tgcttcgatc tttgaatagt ccaagacttg gttaatgagt gtcaacaaga cctcgccgct
     2221 cgagcggata atctgggcgt actcctgctg ctccggcgtt agcgcggttt ccatcaacaa
     2281 ccccgttata ccgatcaccc cgttgagcgg ggtgcgtaat tcgtggctca ggttagctaa
     2341 aaagctgcct ttggcgacgt ttgccgcctg agcgcgtgtg gccatttctt tcgcgttctt
     2401 tatcgattgc cgcagcttga ggttagtctc gctcaactcc gcctgaaaat ctttcaaggc
     2461 cttttctgct ttttctcttt cggtcacatc cacgaagatg gtggcgaggc ctcctttacc
     2521 caaagggatc gcgtagattt caaaggttcg attcgccacc ggtatatatt gaatgatagg
     2581 ccgggttggc cccttgttcc cctcaagaag atacgcatcc agatgggggg gtttttcgat
     2641 gttgaaagct tgagtcacgc gttttcctac cacatcttct ttcttcacat tcatccatcg
     2701 ttcatactgc gggttaatgt ccacgattga aaaatcgatc ggccttccgt tttcgtctac
     2761 ggcgagctcg tgtatcacga cgccagcggc gaggttgtta aaaaaggaac gaaaccgctc
     2821 ctcactctcc cgcaaaagcc gttccgccgc cttcctctcg agagtgtcat gagcagtgat
     2881 ggcgtagtag cgctctccct gtacttcgat cgaccgaacg ttcagttcaa caggggatat
     2941 accgccctcc ttgctcacgt acagcgcgtc cacgctttcc ccgttttgcg caccggccaa
     3001 tcgcgggtgt atctctgttc cctccgaacg cgaagtgaac agaaggtcga gaggggcgcc
     3061 caacatactc ttcgtttcgt atccatacct cgagagggct acgtcgttcg catcgaggat
     3121 ccgaaaacga tcgtcggtca ggatataaca ttcgttagcg tgcttcatca tcagctcacg
     3181 aggtaggcgc agcagatccg ccttcctcct ctgttcctcg agctgcttga gtcgctcagt
     3241 ttctcttttt tgatttatga agatgaatag cgccgcgacg atgacacaca cgaaacccca
     3301 atgtaataga aacggtagat aggttctgcg tacccggttc ctgaagtcat aagaagtgac
     3361 tttcgagaag aagtaccact cggaatcagg aattcgtttc gcggaccaga atgcgatatc
     3421 tccgtgtctg tcttttctaa ctaaagtata atccgaggcc aaggcggact cgatatcctc
     3481 aaggcgaagg tcgccataga ccgttccttc gccgttgacg acgcttcccc cttcacggtt
     3541 cagttgaatg acacgaaaac cgtcatctgt tcttgcaatg aaatagaggc tgagagaggc
     3601 tctctcttgt tctaaacgga ggagatacgg cgacagagac gttaattcga ccctcaggat
     3661 ttgaaaaccg tagagctgtc ggtcggtgtc ctcatcgttg aagatgggaa ggatatggtc
     3721 ttgacacacc tttccagtgt ctgaaacgaa gaaatcggag agctgagcct cttgtcgagc
     3781 gacgcaatag tcgagcgcct tcagggtatc ttcaccccag acaatggtat cgtcatttgt
     3841 attgaacaaa agttctttat tggaattcaa tatgaataga gcggcaatat cacgggcgct
     3901 agagtaggct tcaagccgat caacaaggct tgcgctgggt ggcacattcg gtttaagaag
     3961 ccattcactt aacacgttgg atagagcggc gccttgaaag agaagtcctc tcgtcttaag
     4021 gcgagcctcg gaccaatcca caaggcgctg gagtttaagg tctgatattt cagatactga
     4081 gcgcttttct ccatcgatcc acttcgatag ccctctttcg caaaagaaca atccaagcgc
     4141 taaaataccc actaggagta cgattattag aattatattt ttcaaagcta ttctttggtg
     4201 tgtttggcgg ttgcgcccga tcttttcgct gtttcggctc atctgttcat tccttctgtc
     4261 ccatcagata tgacaaaaag catgcccgaa gccgggcatg ctttcataga gttgtctcag
     4321 atgtaaagtg ggcagctact tagcgaggta atttttgatc tctctttccg cccaggccaa
     4381 gccctcttca atggtccatt tgtcgaagag aacgttgttc agcatattcg tcacggtacc
     4441 tctgatctct tcccaggtcg acggtttcgg atcggagtac ccttggccga tgaacttcac
     4501 cgggatggcc gctttcggat ccatcttcac gaaaattttc cattcggatg tttctagcgc
     4561 gctctttcga accggcacat aaccggtttt aagggaccag aatgtcgtaa ccttcgggct
     4621 gcagaggaac ttcaagaact cccaagccac tttcttttgc tcttgaggaa tggtgttgaa
     4681 cataatgacg tccgttccgg cgaacggggg attgagcttg acgtcgaagg gtacggaggt
     4741 ccaattccaa tcgtgctttc ctttcgatcc gctttcgacg taaggcttac tcgcgatcgt
     4801 ttcgatgaag gacgcgacag aaccctcgcc aaatggcgcg tctaaatacc cgccctgaac
     4861 gagagcgata ttttcgtctt tcagtctttt catgaatgtc agggttttcc gtgtttcttg
     4921 gctattgatc ttcacaacat aagttccatc ctcgttttcg tgtacgatct ctcccccgaa
     4981 ggcgtacaag aaaacgccaa aatggtcgac ggtggttcgg tagccgaacg cgtagcgatc
     5041 gatggttccg tcgttgttct cgtctaccgt caacatccta caaatggcgt acaggtcttc
     5101 catcgttttc ggcgcatcca gcccttccat atcaaaaaca tcggtgttgt agtagaacac
     5161 atagatactc ttgttgaaag gaatggcata gaccttatcg ccccacgtgc acattttctg
     5221 gaatggttcc caaatatctt cccattcttc cttcgtaagg ccgacctccg gatcgttcaa
     5281 gaaaggcgtt aggtcctgta cgatattgct gtgaaggagc ttcgatgtcc agttgctgta
     5341 cgcttgagac aacccgggaa gcgtgttgct ctgtacactt gccagcaact tttgctgcag
     5401 cgcattatag tttccgatgt agacggcgtt gatctgcata tccgcgttct gctgattgaa
     5461 caatttgacg atctcggcca aggtttctcc gtggccgccg cccatagcgt gccaaaactc
     5521 aactgtgacg ggcgcggcga aggcgaaaga gaccagtaat accgcgcaac aaataccgag
     5581 taccagtttt ctcatacaat caacctcctt aagtttttta aagccttagg aacattcgaa
     5641 gaaaggtaca ctactatagg gttttaacct cgtttttcat aaggtgtgcc gctggcagcc
     5701 ggagagcggg tccttcccac aaagccgcca accacgatca gtgtgatcac aaaaggcaga
     5761 agattgaata aaggtttagt agtagaagga atagaaagga tcgggttact ttgcagttgc
     5821 tcgttgagcg cttgggcgac tccgaagagc aaactggccc acatggcccc gaccgggctc
     5881 caattcccaa gaatcagtgc ggccaacgca atgaacccct tgccgccggt catattttcg
     5941 ctgaattggc ccagttctcc cacgctgagg aaagcccccc cgagtcccgc gagtataccg
     6001 ctcatcataa cgccgaaata tcgtatcttg aaaacattaa tccccagagt gtcagctgct
     6061 tccgggtttt cgcccaccgc ccgcatgcgt aatcccaatc gcgtcttgtt gatcaagatc
     6121 cacgtcacga tgactagggc gaaagcgatg tagacgaaag gactcagcgt cccaatcatc
     6181 ttgtataaga aagtgtcctt acccaaaaac gtcaatcgga cctccggaac ccggttaaag
     6241 atatgggtcg aaccgggttg accgaagatc ggctccaata aaaacccggt taacccttgg
     6301 gctagcagga tgagggctgt tgcgctcacg atttggtcag cgcagaaacg gatgctggcc
     6361 caagcgtgca atgacgcgag cccaaggccc gcgaccatcg ccaacagaac gccgatccac
     6421 ggattgccgg tgaacgcgga gcctaagatt ccgaagaagg cgcccaggag cataatgccc
     6481 tcgagggcga tgttggtgac gcccgttttc tcactgaaca atccgcctaa agcggcaaaa
     6541 agcaagggag tcgctgagct taaggtcaac cgataaaaca ccggattagt gatggtatca
     6601 agaagcgctc ttagccagtc catccttcac cttcttcttt cgatcccaac ccatgatcgt
     6661 ccgtaccata cgttcggcag cgacgagaaa gatgatgatc gattggatga tcgttacgat
     6721 gtgtttgggt accccgataa actgcatttg gttcgagcca gttctgagtc cggagatgag
     6781 catcgaagcg aagataatgc ctaacgggtg gttttggccg attagggcga tggagatgcc
     6841 gtcaaagcct tttccggagg tcagagtgcc gtaaaaccga tagtaaagtc ccatagactc
     6901 aagcgttcca gcaagacccg cgagggcgcc gctgatcgcc atcgtcataa tgaggttctt
     6961 agacacttgg atacccccgt attcggccgc atagggattg aacccgaccg ctttgacctc
     7021 gtaccccata gatgttttat acaagatgat gtacatcacg aaggcgacta aaaccgcgac
     7081 gatgattccg taggacaaaa aggtcgcctc aatgctgaag aggatcggca gtttcgcgcc
     7141 gacggcgatt tcggggcttt tcggcgtgcc gctaccggtt gcgaaaggcg cggtaaccgc
     7201 ccaattcgtg atgttactgg cgatccagtt taacataatc gtgctgataa cttcgtgggc
     7261 accggttttc gccttcagaa accccgcgat ccctgcccaa acccctcccg ccagcatacc
     7321 ggtgagcata cagaccggaa ttgcgacaaa catcgggagg cctgggatgt tgacggccac
     7381 aatcgtcgcc atcaaggcac ccatcgccat ttgaccttcc gctccgatat tgaagacgcc
     7441 ggcgcgaaac ccgaaaccca ccgcgagacc cgttaagatc agcggcgtca tacggatgac
     7501 ggtattcatc caagcgaatt ggcttccgaa agcgccctcg aacatcgcct gaaaggcacg
     7561 tcccaagaat gaaaaccgga cacctatttg attccagaaa tcgttccaaa ggttgcccgt
     7621 cgacgggccg ggtacctcgg ctatgaagag gatgatgacc gaagcgatcg ctagggcgat
     7681 cacgaccgcc attaatggaa tgaaaattcg ttgccaacct ttactcatcg tcgtccctcg
     7741 tcacgattcc accctcgtcg tttcactttt tcccgcgctc gcggcttccg tctttcgcac
     7801 ctctgcaagc ggggttccag ccatcgccaa tccgatctct tccaaggtga tctccccggg
     7861 tttcgcttct cccataaact ccccttcgaa cataatcaag attcgatcgc ttagtgaaaa
     7921 gatctcttcc aattccattg acacgagtag cagtccaacg ccagcatccc gtaacccgat
     7981 caaggtccgg tggatgtatt cgatcgcccc gatgtctaaa cctcgcgtcg gttgggccac
     8041 taacatacac cggggctgat agtcgatttc cctgccgacg accatctttt gttggttccc
     8101 tccggaaagg ttctccgcca gcatcaggat actgggcggg cggacgtcga attgcttgat
     8161 caggaacttc gcgcgttcgt acacttgttt gtgccgaaga aatccgtttt tactgaaagg
     8221 gggtagataa tggcgcccca aaagtatgtt atagtagacg ggaaactcct tgaccaggcc
     8281 gtgtttgtaa cggtcctccg gaatgtggga aagccccagt tctcggatat cgcgcggaga
     8341 agagtgggtg atgtccttac cgcaaagctc gatctttcct ttttcctttt ttcgaagccc
     8401 cgcgatcgcc tcgatcaatt ccgtttggcc gttgcccgct acacccgcta tccccaagat
     8461 ttctccttgg tgaacggtga acgagaggtc cttaacggcc tctaacccgc ggttgtcttt
     8521 gacgtgcagg ccgttgacgg cgaggacggc ttctccggcc tttttctccg ccttttcgac
     8581 tctcaacagg acctcgcgcc cgaccatcat attggcgagc tgacgcggag aggtctcgga
     8641 tattgggatg cgtcccgtta cctttccgag ccgcatcacc gtcacgttat cggaaatggc
     8701 cataacttcg ttcaatttat gggtaatgaa gatgatcgtt ttccctttat cagtcagatt
     8761 acgcaagact acgaacaact cgtccgcctc ttgcggtgtg agaaccgcag tcggctcgtc
     8821 taaaatgagg atattcgctc ctcgatacag cgttttcagg atctccaccc gttgctgggt
     8881 tcccactgga atatcttcga tgcgcatatc cacgtcaacg tgaagaccgt aggtttcgga
     8941 cagctcgcgc acgcgttttt tcgccgcctt caaatcgaag aacctccatg agtcgagggg
     9001 ctcttctccg agaacgatgt tttcggctat ggtgagcggc ccgactagca taaaatgttg
     9061 gtgaaccatc ccaatacccg ctttgattgc ctccgctggc cccttgatga gggtctcttt
     9121 accgtttatg tggatcttcc ccgcatccgg ggtgtagagg ccgtagagca agttcatcaa
     9181 cgtggatttg ccggccccat tttctccgac gatcgcgtgt atctctcccg ctcgcacgta
     9241 caagtccaca tgatcgttcg cgagaaccgt cgggaactgc ttcacgatcc ctttcatcac
     9301 gaccgcgtaa tgaggatatt cagaaggttc tgggatatga aatggcatct tgcctccgat
     9361 cgctctataa ggggtccgtg cgcgacccgc cgcgtccgga cccccgatga gtaatggata
     9421 atttacttca gaaacgcctc gacagcggct tcgctgtccg gtatgacaaa gtcgcctgcg
     9481 atggcctgag cttctagacc tttcagcatc acgaggatcc acttcgggat gatatttcgg
     9541 gtgtacttca tttcgctcaa cctggcgccg ccctcgataa agccgaggtt ttcgtgaccg
     9601 ccttcgaaga agccttcata cgcgtcctta acggcgtaat aagaggccat gtcgactcct
     9661 ttcgtcgcag aggtcaggat ccggcctggg gccaaatggt cttgatcgac gtccacgccg
     9721 atcgcgtagt acccttctcc ggcggacttc gccgcttcaa aagtccccaa accgcaaagg
     9781 ccggccgccg cgaaaacgat gtccgcgcct tgagaatact gcgcttcggt catcgttttg
     9841 cccttctgag aatcggtgaa gctctgggtg tagccgatga gaacggtcac gtcgtcatcg
     9901 aacagtttgt tccacatatg gattcccgac cgatagcccc actcgaagcg ttggaccgga
     9961 ggaatcggta tcccaccgat gtatccgacc acaccggttt tggtcatcgc ggccgctaaa
    10021 aatccggcta aaaaggcgca ttcctgctct ttgaaggtat aggtcttcac gttcggtatc
    10081 cccatgtcac cgatgtcgat accgacgaaa gccacgttcg gatactgagg agcgacctgc
    10141 atcaaagcgt cttccatcat gaagccgacg gcgacgacaa cccgggactc tttcgccgca
    10201 ttcgacaggt taggcacata gtccgcctgt tcgtaagact gaaccacctt cgcagtgatg
    10261 cccaactctt tttccgccgc tttaataccg gaccaagtac cgtcgttaaa ggattggtct
    10321 cctagacccc cggtatcggt gaccatggtg acgatgaagt tcccaaaaaa cacggttgag
    10381 acgagaagaa ccagcgcgag cacaaacagt cttttcattg tttctttccc tccttttgat
    10441 atgttttcca gctaaggtag atagagaaca aaaagcacgt tatggcaaga actaatacga
    10501 acattccggg atggttttct gtgaggggga gcaccgggta ccccttttcg acgagtagat
    10561 ggatatcgct tctgaaaagc atcgcaaaaa agggaatcca cgagcaggcg tagatggcca
    10621 aaacaacgcg acgcttggaa cgtaaaagaa ctaaggtagc cgttgccagg gtaatgagca
    10681 gcaacgcgaa actcccttcg accgattgga agaggtagaa gttcgggctt ctaaaatcgg
    10741 atcgggacgc ttcggaatag gcttgcatca cggaggtctc caaagggaga aagcgcgcgg
    10801 atttccactt gctcaaaaga taggattccg tctcctctct gagctttatc ccagaaaacg
    10861 gcatcgcctt attcaagcgc ccgcgcagga tctccgtaaa ccggggagtg gcttctcgac
    10921 tgatcaggta ttcgttgaag gcttcctcgt aaagaccgcg cttccattcg aacaactctt
    10981 ctttcaagac ctcgtaaacg gcggattgat agaacgtctc ttggcggtcc attctgagcg
    11041 cctcgggtgc aacccagtag ggaaaaaacc acagagcccc tatcgccaaa tacatccaaa
    11101 ggtgcgtcca ttcataagcc ccacgtctct ttttgttgga gactctggac agccaaacca
    11161 gcaaggagaa ccccagaaga gaaaaggtga ggagtccata aacgaaggct tcggaatcgg
    11221 agaaaaggta accttgagta tggtattgca cgaaggcttc gaagagaatg aggagcgtca
    11281 agatcaatgc gcgccatttc ttgaggaaga tcacggcgag gaggaccaaa ccgtaggcca
    11341 aaaaccggta tgcccaaagg ttcacggctt taggagccct atccacgaag tctttcgcga
    11401 gtttcgtttg gtcgggtagc aaggtcgtat agatcggggc gcctaccgtt tgcgcctgtc
    11461 tcttgatcag ccgatcgagg tcttgatcgg atatggtctt cgcagtttcc gcttttaccc
    11521 gcgctatcgc ctgtttcaag ttctcttctg tttcggaggt gaagccggag acgatgagtg
    11581 ggaagagcgt ctcgtctggg gtgaaggcgt accgtagcct cgttttcaag gctttagcag
    11641 gaggatcgat cgaaaaacgc gcatctggag gcgcggctac gattccgacc ccgtgcgcta
    11701 tccaatggga aaagtattgg atagccgtat cgcgcaaatg gttgttcgct tggttatagg
    11761 tcgtatagta cggggcgaaa ctgttcaaac ctccaacggt gaggtctcgg ttgaacaggt
    11821 gcgcggataa ataggccaag taagccgata gggataagtc ctcgttgatt tctccggtct
    11881 tgatgctgtc gaaaacgagt tccagaagct tgttgacgtc atagaaacgc tcatcgtcgc
    11941 gaatggggcc gatcatggcc aagcggtatt tacggtaaac ggagatatcg tttatcgctt
    12001 cgaagatctg gttggattga tcgagaggta gagcaatcca cccttcgaat cgctcgaggg
    12061 attcgtcaaa gcttataccc caggcgataa cgaaaaacga aagaaagaat atgtataggc
    12121 ttagtttttt cacccgtatc tcctccgagg taatctccca tcctagactc gccggataat
    12181 tataacatgg tcacaagaaa atcagaaccc ctccggatac gaccgaaatt agaaagtctt
    12241 cagaatgaaa aggatctata gattacccac cggaaaagaa gagaaacggc gattcccagc
    12301 gtggggctga cccaccagag cgggggggtc atttttctaa aaatcaagcg gtacgacagg
    12361 ccgcacccga tcgaagccaa agccaacggc cagttcagag ccaataagaa aatgaagatg
    12421 gccagacgtt cggaaatccc atccgactcg ttctccatct tcggaatcca tccgatcatg
    12481 cggaagattt gagtggggat gacggagacc gagaccatac cgaccaggta catcgcgaag
    12541 gcgggagtga tataggagcc tgtaaaaaaa acggagctga atataccaat cgcgaggaaa
    12601 gaaagccaag aaacggtctc tacccaccat acctttttgt ttttgaaccg aaggcaatcg
    12661 atcaatccgt gcaaaaccaa agaaccgaag aatagggtga tcccataacc ggaagaaaac
    12721 acggtgtcga aggtgaaagc gaggaacacc acggcgaacc aagcggcgtg cttgagaatg
    12781 gcccacgagg aaccttctcc gtacagcttc atcgggttcg tgaaagagta gtcggccacc
    12841 gtatgcgcta aaaacaaatg cagggccagc attcactaca cctccgtctc tagagcgccc
    12901 accgcgcttt tcaaagaaac aatcgccact tcgagctggt catcgtccgg ctcggctgtc
    12961 gtcagccgct gcaggagcat cccaggccaa gccaacgccc gaaagagagg gttatcgagg
    13021 tgtttggccg tgaagcgtaa aaattcgtaa gagatggagg cgatgacagg aatgaagaga
    13081 atccggatga ggagtttctg ccagaacccg acctgccaaa tggagtttgc gatgattaaa
    13141 acgatgatcg cggcgatcat cgtaataaaa aggaagctcg ttccgcaccg cgggtgtatc
    13201 cgcgaatact tgcggacctc attcaccgtc agctcgacgc cggcctcata ggcatgaacg
    13261 gttttatgtt cggcgccgtg gtattcgaaa acccgtttca catctttgga acgggatacc
    13321 gcccaaatat ataaaacgag aatggccacc cttatgatcc cctcaaatag ggcgaaagag
    13381 actgggtcct ctttaatcgc cggtatcagc tgggccacga ggaagggcgc cagtacgaat
    13441 agtccgattg ccagcgctag ggcgaagagg atcgcgccca cgatatcctt ctttttcagc
    13501 tcttctcccg tatcctgtga ggagacctcc gccgaccaac tcaaggctac gatacccata
    13561 tacatcgaat cccatagtac gcaaacgcca cgtataaacg gggtccctaa aaccggaaac
    13621 cgtttgatca acatcggttt gtcatattca cgggtgacga ttttccggtc cggttgacga
    13681 acggcgatcg cggttttttt cgcgcgcatc aacacgccct ctatgaccgc ttggcctccc
    13741 gcagccatac tctcttcaac ttgtagtaag ttcattcaac gccacctttt ttccgatttt
    13801 ctccgcgtaa actctttcta ttaaaaccga gaagacgcgt ttggccttga tatcttcgtc
    13861 cgtttcagcc ccgataaaaa acagggtcat cttttgacga aagctctccc gttcttcttc
    13921 ggaaaacgac aaaatacgcc ccaggatttc tctgaggcgt tcatcttcac tacacgctat
    13981 cgcaatctgg tttaactctt cttctgaagg agtgtagaaa tccctcattc atcaactttt
    14041 ggtttctcta ccgctgtcac tttgaccgag atggccgttc tggtttctcc gtcgatattg
    14101 acgtcctcaa agctgggaac catatagagg tctttttgct gttcgtccaa aaaccgtctc
    14161 gcgatcgcca ccgccttgac cgattgattc accgccccgg ctccgatcgc ctgcagctcg
    14221 acgcattggt tcttactgag ctcgcccgcg atcgctccgg cgaccttgtt tggattggaa
    14281 ttggagctaa ccttcaacac ttccatagtt cgttcctcct ttgactggtg gatcggaaaa
    14341 cgcaaacgat aggaaacatc agtttacggt tcggaactat tataccatag tatgatggct
    14401 aagcgacgta ggttaaaaaa ccttcgggct taggacgttt gctcggatat tgcaaaacta
    14461 cctaccgtca aaacgccaaa agtgatgacc ccgttggaaa tggctttttc caccaccttg
    14521 ccggtgaaat ccaagatatc ctttgtatcg acctctttgg ctttgaagct cagagtcgta
    14581 tccgcttcgg cgtattcccc gccggtgatg ttaaacttgt atggctccgc tccggcccgc
    14641 ttccctgcgg ctaatgctgg ggtaagggtg acctggccac cgatgatttt tgagttcccg
    14701 aagagccatt caaattctag cgtaatgctc acattgtagg gacccgcagc cacctggccg
    14761 gcggcggtcg tcaggttgac ggtccccttc catttctttc ccaccggcgt acccttaaac
    14821 aacacacaag aactgatcac gaagaccaac gccaaaactg caatcagaac ccctaatcct
    14881 ctttttttca aagtagacac ctccagtata gtttttttct taaaactcct ctatcggtcg
    14941 aaacggatct ccaatcactg agtcgccgcc cgtcggagaa gaagcgaacg acctttcggt
    15001 gtgatcgctg aggatgacct cacgcgcgcg ccgccgaaaa cgcgcaacct ccgggttaag
    15061 gacctttttt acgccttttg ttctccaatc gttgaacggt cgtcaagaaa tgaacccaac
    15121 gtcgatagac ctctcgactc cacgactcaa aaacgggcgc ctcgacttcc ctttcgaaga
    15181 gctcgaagcc gatcagatat ggatacagga aaaaagagca attctcatcc ctcttcaact
    15241 ccgcgttcga atacggagaa tcgtttacca aaagaaaaga ccggatcata tcgtcgagct
    15301 ttagtttgtc gatcgggcgg aatgaagtcg taattttatt cctcaagcac gagggacaaa
    15361 gacgcaccgt cttcccgtag tattcggaat ccaacgcgta gacggtcacc tgttcatccg
    15421 tcccgcagta gtcacaacga tccatatttc tccctcgaaa atcgattgat ctggttcatc
    15481 acagtctcca ctcgttcata ggtcagtcgt tctatcgcgt cggccacggt gtcgatcgtt
    15541 tcgttgatga tcgaccgctc cgtaattttc aaggggcgca acacgtagtc cgacaggtcc
    15601 atcgctgggg gtttggggcc gatccctatc cgtatcctcg cgatttcatc gctcccggtg
    15661 acggacaaaa tcgatcgtat acccttgtgg cccccatcgg accctttcgc ccgtatccgc
    15721 accgcgccca gcggtaaggc tacatcatca tagatgatga cgctttcgcc atcggagtag
    15781 ccgaaatgtc cctgagcgtg gcggtaggcc tctccgctac ggttcatgaa ggtgagcggt
    15841 ttgatcagta gataggcttg accaaatcga ttccggcaac tccaatactg gtataacgtc
    15901 ggaaggaacc gcgactccaa aatctctttc cggtgtgtgg tcacgaaatg gtcgatcact
    15961 tgaaacccga gattgtgttt ggaatcatga tactcggttc cgggattccc aagaccgaag
    16021 aacaccatct ccgtgtctcc ttcggtatta tccgactctg tcaagcgctt atttctcttc
    16081 tttcttttcc ctaatcactt ctggctgggt gacctcttcc tcaccggcgg gctcttcttc
    16141 tacctcttcc ttcggcatcg tgacggccaa gaggatctct tcggggtcca ggagcggttt
    16201 gaccccgggc ggtaggtgga gatccttcac cttcaagaaa tcgcccagat cgagattggc
    16261 gatatccacc gagaccattt ccggaatgtc tttcggaagc gcttccacct cgaccgattc
    16321 gtggaaggta tcgacacgcc ctccttggtc aacgcctttg ggcgtgttct ggtattcgat
    16381 cggcacctcg atccgcatca cgtggccttc cgagggcacg aaaaagtcga aatggatcgg
    16441 gaaatctgtg accttatgtc gctgaacttg tttcaaatag gcccttttca ccattgagct
    16501 cccatcggac ttggcaattt tcaggtcaat agaggtggtg gtcgagacct tttggatcat
    16561 aatcatgaac tcttctctgc ccacctcgat gttcaacggg ccaaagtccg ggccataggc
    16621 tgttcccggg agaaccttac gcgccctcaa tttccgggag ccggatgttt ttaatcgggt
    16681 cctttctgct acttgtagtg cttgctttgc catggtactc ctcctcgttt atctagaaac
    16741 gctattgttc tcgtttacct gaaaagaatg ctgaccgaaa ggtttttccg tactcgaatc
    16801 acggcttcac ccaacaaatg agagatgtcg aggatcttaa acttgtcggg caaggagcga
    16861 tgcggtatgg tgttcgtcag cagaatccgg tcgattaaag aattttggag caattccgcg
    16921 gcgtttcccg atagcaaacc gtgcgcggcg caggcctgaa cgctcactgc gcccacattc
    16981 agcaaggttt cagccgcccc tacgagagaa cgccccgtat cgatcatatc gtcgaataag
    17041 atgaccttct tcccctttac gtcacccacg acattgacga tctcggccac attgtcgcgt
    17101 gggcggcgtt tgtcgagaat ggccagcgac gtcttcatct tctccgcgaa tcttctgact
    17161 cgcttaacac ccccgacgtc gggagatacg gtcacgtagt cctcggggtt gaaagagtct
    17221 tcttcggtga aatagttgca gaaaaccggg aaagagcgga gattgtccag cgggatatcg
    17281 aaaaacccct ggatttgatc cgcatgcaag tcgaccgtca cgaccctctt ggcccctgcg
    17341 acggtgatta ggttggccac caatttggcg ctgatcgggt cgcgtcctct ggcttttcga
    17401 tcctgacgcg cgtacccgta atagggcatg ataatcgaca ccgaattggc cgaggcgcga
    17461 caaaaggcgt cgatcatcac caaaagctcc atcaggttgt cattcacggg agtgcaggtg
    17521 gactgtatga taaaaacgtc gtgaccgcga acggtctccc cgatcttgat gttcacctct
    17581 ccgtcggcga actttccgat atcgatctcg cccaatttga cagacatgta gtccgccatc
    17641 ttctgagcga gctccgggtt tgacgatccg gcgaaaactt tcatttcggg acgattataa
    17701 ggcatacccc tcgaacccct tccagaaaaa aagactcttt gtcatcagcc gcttcacggt
    17761 cgtttcttgt tcctctccct tatgacggcg gctcttccag gtttctgtat ctgttgtcct
    17821 cgggccagcg ctaatgcgtc cttctcgacg ttttcgacga tcacggagcc ggcgcccacg
    17881 atggcgccct ctccgatcga aaccggggcg acgagggcgg tattgctgcc gatgaaggcg
    17941 ttgttcccga tttgcgtcgg gtatttatga tacccgtcgt agttgcaagt gatggtaccc
    18001 gcgccgatat tcacgttctc tccgatcgtc gcgtccccta aatagctcag atggtgcgct
    18061 ttcgacccaa cgcccatcga cgtctttttc agctcgacga agtcccctat gctgacccct
    18121 tcggagagct ccgttccttc ccgtatcctc gcgaaagggc cgatgcgcac ccgcttggat
    18181 acctttgccc cacgcatatg ggaccggtcg atttcgcatc cgtcaccgat cgagcagtcc
    18241 gtaatctggc tgaaggggcc gatgacgcag tcttttccga tcaccgtctt gcccgcgatg
    18301 aagcaatacg gttgtatgat cgtatccata ccgatctgga cgtccgattc tatcgtcgtg
    18361 ttttccggat cgatgactgt taccccttcc gccaaccaac gatggttgat acgaaggcgc
    18421 atcagtctgt ccaaagtcac gagctgacat cggtcgttta cgccgctcac ctcgaccggg
    18481 tcgtttatgg ttaaagccgc aacttttccg cggttcctta caaggatctc tacggtttgc
    18541 ggcaagtagt attctccttg cgcgttgtgg ttatccagtc tgtccaatgc ccaagaaagg
    18601 gcgcgcgcct caaacacata gaccccggcg ttcacttctc tgatagaacg ctgctcgggc
    18661 gatagatcct tctcttccac gattcgttcg acgtgtccct gggggtctcg aaagatgcgg
    18721 ccgtatccgt gagggttcgg gttgtccacc gtcaacacgg ccccgctgag ctcttttgcg
    18781 catagggcca actctcgaac cgtctcaggg cgaacgaaag gcacgtctcc ggaaagaatg
    18841 gccaccgact ctcccttctc acatcggtcg atcgcttcct tggccatcaa aacggcgtgt
    18901 ccggaaccca aacgttccct ttgaaaacag atctccacgt tctctaaacc ggcttctttc
    18961 ttccagcctt cgatataatc gaccgcttct tgcatatccg gatggatgac cacggcgaga
    19021 cgattcggtt gccctggatc aaaggccttc tggcaagcac acaagaccca tcccaatatg
    19081 ggttttccaa cgagcttatg taataccttc gggcggttcg acttcatccg ttttccgaat
    19141 cccgccgcaa ggacaataaa aagcatggtc tctacctccc gtgaaccaag gcttattata
    19201 ccaaaaaaag gaaaattctt tacaaccgga aatcgagaaa aagggtagcc ctttttcgaa
    19261 tggcccgttt cacggatgag ggcgctccct caggtggtct gatcggtcaa tcgccgttcc
    19321 ttttcgatac cgacggccct tctcgtcttt tctgcggttt tgatccatct cccgctcgcg
    19381 tatacggtca acttgaggat agagcgcgtg atgaagtcgg tcatcatccc tatccacgcg
    19441 cccagcaacc cgagattgaa aaacttgacc aagatgaaac cgatcggcaa acgtaaaccc
    19501 cagacggcgg tcaaagagat gaagaacggg agcttcgttt ctcccactcc gcgcaagcta
    19561 ccggtgatcg cggagtccgt agccaaaaag aattgcacga gtccgatgat ccgtacggga
    19621 accgccgcca tatcgatgat atctttatct gcagtgaaaa tacggatcaa gaactccggg
    19681 aacagcgaaa tgaagatacc ggcgcaaacc ataaacgcgc atgcgatgat ccacccttgt
    19741 ttcacgatgc cgcgaaccat acggagatcg tcccgaccgt tgtactggcc ggctaaggcc
    19801 gttaaggcca ccgaaacgcc aaaccccggc ataaaggaaa tggattcgat gttgatgccc
    19861 acgcggtgcg ctgcgtaagc ttgatctccc gcgatcaaca agatgttcgc gaaaacgagc
    19921 atcccaagcg aaaaaaagaa attttccata ctcgtgggca acccgacgcg taagatctct
    19981 ttcgttcttt tcgagctaaa gcggcgattt tctttgacac gcaactggaa ccgcggcgat
    20041 cggaagagga tgaccccgag gatcactaag cccaccgcgc gagagataac ggtcgccaaa
    20101 gccgcgccct cgatccccat cttgggcatt cccattttcc cgaagatgag gatgtagtcc
    20161 aagagcgcgt tcagggagtt cgcgagaata gacgcataca gcgggcttct cgtgtctccg
    20221 gatccgcgca gaagagcgga caggacgaac atcagggaca agacgacccc tccgagcagg
    20281 ttgatctgca agtaggaagc ggccatcccg cgcacctcgg ccgaggcgcc ggcccctcct
    20341 aaaaaaacca aaccggaaga gatcagcgaa aagcatgtga tcaccaaacc taagatcaac
    20401 gctagataga tagcgttcca ggtcgcggaa cggatggcgt tctggttctt cgctcccacg
    20461 gcgttcgcaa ctaaaaccga acttcctgtc gcgattgcga cgaggaacgc ttggaatatg
    20521 aaaaagacct ggttggctaa acccgcggcc gccattgcct gccagttcaa gtaccccaaa
    20581 aacgccgtgt cgaccagtcc taaaaccatc tgtaacgcgt tttcacccat agcgggcaag
    20641 gcgatggcca gaacctccct gcgtacctcg gctacttcct gggcggatag atcttgtttg
    20701 aagatattga acagtctcat cgttaagccg ttatcgtttt ctcacgccag aggcaagaaa
    20761 gacgatctct ccttttctat tttatttgta tattttctaa acttctaaaa atatccgcaa
    20821 gaagtctttt atctgtgtgg atcaagttat aaggtgacat aacaaagagg ttcatcgctg
    20881 gttaagtggt ttttacgccc ccgcgatgct gcgcactaga acactcccag ataaagagat
    20941 taaaccatgc cgaactaaag tttagcgttc ccagggcctt cccaagaatc ttctctctgt
    21001 gttctctgtg cgaaccctct tttttcgcgc cgtcgcgaaa agaagggacg cttacgccgc
    21061 gatgaccgtg tggagttatc gcggggtagt ggttttcacg cccccgcgat gctgcgcact
    21121 ggaacgctcc cagttaaaaa accatgccaa actaaagttt ggcgttccca gggccttccc
    21181 aagaatcttc tctctgtgtt ctctgtgttc tctgtgccct ctgtgcgaac cctctttttt
    21241 acgcgccgtc gcgaaaagaa gggacgttta cgtcgcgatg accgtgtgag gttatcgcgg
    21301 ggtaagtggt ttttacgacc ccgcgatccg cctttagtcc cttctttcta cgccgcgatg
    21361 actacctgga ctaaaaatcg tgccatcgat tctcgtcttg tttcttgata acactccata
    21421 gcttgaccca cgccttttat ttccccaacg ccctttgcaa ggcttctatg atcatcgcgt
    21481 cggccggtgg gaattcttgg ggcttcatct gttcgggttt cagccagcga ccctcccggt
    21541 gttccgtgag gcggatcgtc ccgccttttc tctcacattc ataaaaggag atctgcagtt
    21601 gaccgaacgg ataggaatac ccgtttcttt ggaggaatcg cgtgggctcc acgacgatcg
    21661 agagctcttc ttgtatctcg cgcttgagcg ccgcttcctc cgtctctccc ggctcgacct
    21721 tcccgccggg aaactcccag taatccccaa aataaccgcc aggctttcgt ttcgcggtaa
    21781 atacccgtcc cgtttcgtct ttgatgatgg cggccacgac gtgaagtggg cgttcggcgt
    21841 atcgagccag aacctttcga aaggcctttg ggatcgcgag cgcctcgatc tcccgagagt
    21901 cgaaaaatgc aaacgagcgt tcgggatcgt gctcgtcgga gcaggataat tcacaaaaag
    21961 cgtgaaaaac ccgcatccgc cagcgttgat gtgtgaagac gtgctgctct acgaacagct
    22021 ccctcgggta aatcgtctcc aaatcatagc gcgtggctag gtaacgcacg agggattggc
    22081 cgggctcggc ccccagaaag gcttctgtcg ttggtaagcc ccaaaagccc ttgagcagcc
    22141 ccgtttcatc tctcctttcc agcatcagcc gttggttact ccaaatgatg cccacttcca
    22201 tctcttttgt ttccaaggct ctactctttt ccttcaatgg ccgaacacgc tgcagacccg
    22261 cttggagagc gacacaatcc cctgcgatcg ggcacttgtc gcacaaaggc ttctttggac
    22321 tacagactaa ggcgcccagt tctatataag cctgattgaa gtcggaaggc gaaagacctc
    22381 cctgaaaggc gatctgaata tagtcgcgag cgaggttatg cgttcgcctt ttctcatcac
    22441 gcgcattcgc aacatcgtca agcgcataag cccgagaaaa aactcgatga acgttaccgt
    22501 ctacgctggg gaatggcctt tcgtagatta tgctgagtaa agcgcctgcc gagtaggcgc
    22561 cgataccggg cagcttgagc aatgcggcat agtcttcggg tatcctcccc ccatagcgtt
    22621 cgacgattat ctggcaagtg cgtcgcagat ttttcgcccg ggcgtagtat cccagccctt
    22681 cctaactttt cagcacttct tcggtttcgg cgcgcgccaa agcgcgcgct gtcggaaaac
    22741 ggagcaagaa cggttcataa tatcgaatcg ccgtctcgac tccggtttgt tgcaacatca
    22801 actcggacac caatatcgcg tacgggtcgt tcgtccgtct ccagggcaag tctctcctgt
    22861 gggcgcgata ccagtctgtc aaagctcttt gaattgcgaa gattttccta ttgctgtcct
    22921 ctatggaacc gtctcccaat tccagcgatc aatttaaaaa agagaccgac ataatcatat
    22981 ctagatagat gtccttccac gtttcggttt ctcgtgtcgg cgcgatcccg atgaaaacga
    23041 tgtactctcc ttgaaaaaca tcctcgataa tggattccga gaagagcata atccaagcgc
    23101 gcatatcgga aaaagacagt tcgtaagttt tgacgaatcc caattgcaaa tccaaatagg
    23161 gagttttttt gatcaaattg tagttgccca accccccgag ataggatgag aggaggtctt
    23221 gatacagctc atcgcagagg agctcctcgt cgatataccc cacgaaaagt ttggcgacgg
    23281 tcatatcggt tgaaacgaag tcgaagccct ctaattggtc cgcatagaag taatcgggtt
    23341 tgagctccgc ggggtagaaa acataaaaag aatctccgta ggcggtcttc caacctcttt
    23401 ttgggctatc cagccactcc tccgcagaca gcctttggat caggagcgcg ccgaagtctt
    23461 tgcccgcagg cagtccggtt gttttcggag tctctttaac tgggatcgtg acaggttctg
    23521 ttgttttagc ggaagaggcg ggcgcgtagg acccgatcaa cgagccgccg atcccctcta
    23581 tccaagcggc caaaaggtct tgcttttccg gagcctgata caaacccggc tccacgatca
    23641 gcatcacgac cgttccgctt ttcacatccg tctgttgggc gcctttttga tagagcaaca
    23701 gaaccgggag cgcaaagagc tggtccgaaa gagtcagctt cgtcaggtat gcggttcggc
    23761 cgttctggag gtcggaatac tcttcgaaag acttgcttcc actcgtacga acgccataca
    23821 gagaggttgt cagatctacg agcagttcat ccagcttata ggcatccaat tgataaacga
    23881 agagacgggc gaataattgt cccgaactgt ctacgatctc cgcgtcgaaa ttcgagccgc
    23941 tcttggaggc gttcaaagaa aagccggcag ggagtttgag cgtgtacccg cccacaacgg
    24001 cttccttcca acccgggagg ctgggtgcct tccatccggc ctgaaaagag gcccgtaatg
    24061 ggtctatcgt cttgagggac agtttccccg tttcggtctt ctcggtggca gcttctgtcg
    24121 tcgtcgcctc tacggtcctc agaccgagta ggttatctga aaatccaaga acacacaaac
    24181 tgaagattaa acacaacgcg
//
LOCUS       DAOPXN010000009        23857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2106_length_23857_cov_5.933325, whole genome shotgun sequence.
ACCESSION   DAOPXN010000009 DAOPXN010000000
VERSION     DAOPXN010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 23857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23857
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2106_length_23857_cov_5.933325"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            642..3920
                     /locus_tag="PKM49_01065"
     CDS             642..3920
                     /locus_tag="PKM49_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY81435.1"
                     /translation="MEDNSFCLLVTDLNGRVKYANSKACQRMGFFEGANESLLQERLI
                     STLLPDDYFFGSLEEFARTKGWERKIRKVHRDGTIRWESITVVPVFDAQDEFAYAVVL
                     IEDISDQKGMADALAVSEKRFREVAEHSHTVVWETDTEGLFTYVSPVCERVWGYRPEE
                     MVHKMHFYDLFPPDEREEAKNEGLSLMREEKALTNFENRSMHKDGRLIWLSSNAVPVY
                     DSEGLYIGYRGTENEITERKTLEEETHRFRATTDQANYGTAIAQMDGTLNYVNHALVK
                     MLGTDEHALLGKNLFASHIKERSERIAALEEKIRIQGGFSSEEVQFVRTDGTSFPALM
                     SVKVVVDARRRPLFQSLTLMDISKLKEYEKLLTLLSHAVEQNPAYVIITDTSGHIEYV
                     NPAFTEKIGYALEDVKGSRAYILRPEHHSPTQYRELWETLESGGTWRGEFRNKRKNGE
                     VFWEYSTISPVVDQKGEATHYVVVNTDITEQKVALNAYKESETILQAFTRTSREAIFM
                     IDSEDTVCFWNPSAYEIFGYREEEVLGEKLDSFIRPYVSSVSETPSFLALLKDNQKAP
                     GAALLDLEVYTKSGALVSVEFSQSITFLGENRYRVVVGRDVRERKQAEADRIAREAAE
                     ASNRAKSAFLSNMSHEIRTPLNAILGFAQILNRDASLSSKQIDHVQTILRSGEHLLKL
                     INNVLELSKIEAGKLTLSAAPFDLHNCILDLERVFRFKAEEKGLQLLVERAPDLPHYI
                     LSDEGKFRQILINLLGNAVKFTQTGGIALRARTVPDPTRTVAKPESLLLQVEVEDSGI
                     GISEKALSRIFEAFNTSVENSSASGTGLGLAISKRLIELMGGTLSVKSKVGTGSQFRF
                     EIPITPAEGSFYKSATPTAEIKALHPSSKPVRVLIVDDLTDNRTLLRALLEPIGFETE
                     DAVDGKEAIELFERWSPHAILMDMRMPVMDGYEATRLIKGTEKGSKTAVIAVTASAFD
                     EDEKAVLATGADGYIRKPFRPDELLSELGRLLPIVYLYESPEEETPKKTAGVARLTKE
                     DLAILPEPLRQRLFRYVEEGEMTELKNTLAKVEALNPEVSQALLALAKQYDYERLTDL
                     LKDT"
     gene            4773..5147
                     /locus_tag="PKM49_01070"
     CDS             4773..5147
                     /locus_tag="PKM49_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81436.1"
                     /translation="MKRKILLLTLICAIIVFSILFSVLGCTTQPGPGGSFSVMVFNEH
                     DSHTLAIRINGEHSGESKKGQPYSQGGFSAADRVTIFNTRTGVYLKDELTGQNSWTIH
                     GNLFFTYWYGPYIDFVYTIDEH"
     gene            6405..6755
                     /locus_tag="PKM49_01075"
     CDS             6405..6755
                     /locus_tag="PKM49_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81437.1"
                     /translation="MQGLSLDFEATYGPPFMKPFRFRAGVGFYELTKICLLAGMEIPI
                     FERMNDFRAKLWGVYLIPEVALGFSRWDASLRCEVLIPVNVLGGIQIGLGVNKDLKLL
                     VSLSYSTGLYPLVK"
     gene            6787..7161
                     /locus_tag="PKM49_01080"
     CDS             6787..7161
                     /locus_tag="PKM49_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81438.1"
                     /translation="MKKLVLLLSLVVVALLMSSCTYFFYSQDGHLGYYPGAVGVPVGS
                     FSASREQWYLLSPLWFPFSEPNKHLDEMIIPEVVKYNGSFAKDLKIGYGFTFIDYLIT
                     YFTGGVLGRNTITVDGQVFRNF"
     gene            7317..7778
                     /locus_tag="PKM49_01085"
     CDS             7317..7778
                     /locus_tag="PKM49_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81439.1"
                     /translation="MKRVVIGAVLVLCVTIAFSQFSLGVMVGQPSGLSARWQLSENLS
                     LDFGAAYSFLWTTGIHIHVDAVFFERTLLRISDNVIPVYFGAGARYLGAYWGPIGITT
                     LSARVPIGVLYPFHVSGNVNLEVFAEVVPTVMVIPAFAFDVSFALGARYRF"
     gene            7839..8948
                     /locus_tag="PKM49_01090"
     CDS             7839..8948
                     /locus_tag="PKM49_01090"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HNY81440.1"
                     /translation="MPIAWVLAQVSIMFFGFATVVPSIFGETGGATIHTYTLNNANGI
                     SVRVLDFGATILSISVPDRNGQVVDILLGYSTIEEYVLDKSYFGSTVGRYSGRIANGQ
                     FALDGMVYTLPKNDGQNTLHGGLKGFNKRLWKAEVEAEGPFPAVRMTYISPNGEEGFP
                     GRLTVSVRFTLTDDNELILDYEATTDRPTVVNLTNHAYFNLRGEGNGDILSHSLQIFS
                     DYFAEMDETLIPTGAFHPVDGTALDFRAPFLVGARLDPCDQWLSSAKGYDHSFLLREE
                     NAGACVRAARLYEPESGRALEVWTTEPTIHLYTGNVLQAVSGKAGTTYGPYSGLCLET
                     QHFSDSPNRPEFPLTRLDSGDRFLSRTIYRFSIDP"
     gene            9011..9535
                     /locus_tag="PKM49_01095"
     CDS             9011..9535
                     /locus_tag="PKM49_01095"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNY81441.1"
                     /translation="MQHLAISKMAEDIYLALSKGEVILSTRDSSGRPTGMTVAWGFFG
                     CLWNEPYFLAAARPYRYTWQGIRDSRRFTVNVLSDAYAEGLRYFGTVSGRSEDKFAKG
                     YFHINEALHDYCAPIEEARLLLDCTVVTANQLQPFFIPEEAIQRFYREDNGYHTIFYG
                     HIDRWHRSEATGEN"
     gene            9535..>9748
                     /locus_tag="PKM49_01100"
     CDS             9535..>9748
                     /locus_tag="PKM49_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81442.1"
                     /translation="MSYSFETLLVEDCHFDENIFPDLPMIILTSMENYTTSIKAIRHG
                     VLIKNVSRRRKKKKRLRHDDYVCYRGV"
     assembly_gap    9749..9848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10098..11843
                     /locus_tag="PKM49_01105"
     CDS             10098..11843
                     /locus_tag="PKM49_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81443.1"
                     /translation="MKKFVFVVCLLVALVAFATDFKVVDPMFTPEPKPNKGGTLTLRL
                     SGSPQSWLFYGSLDNNAYTVCYNLFDPLIEANPVTNELGPGLAKSWEVSEDGLEVTFH
                     LRDVNWTDGVPFTADDVIFTFENFVMNKYARGNSIDRFTLQGKTIEWVKVNDKTLVAK
                     LPYPYGAFFFVTSHVLIYPKHVVAPLIDLSDPDSVNKALTTDTPVNKIVGTGSFILSQ
                     YVVDQKVVLKKNPNGWKKDQYGNQLPYVDELVYLIVKDAEVNVAKFLAGEIDYMSLNA
                     RDYSNFKIKEREGAPIKVFKALPTGNTPSPVHIAINFDNKDPELKALFRTDAFRFALE
                     YALDRDRIIDQVFNTLATYGDSVYPTNAMWYSKYVDKLRRPYDLKKAAAILDELGLTD
                     KNKDGWRELPSGKILEFTIIMNNASKSEQDIALIYQEDLKSIGVKLNTQPLDSSIRSQ
                     KATGGDFDLCIWAFGTQPDPQLRKAIWQPGGSLYYWHLSLFNKDNPDAPFMDDMTSWE
                     TVLWYVWEKAALEMDLARRQHLYYIYQDIYAEMLPVIFVVREMDVRAGNNKLGNMYQT
                     ESGAFVFTNYTVFKK"
     gene            11910..12878
                     /locus_tag="PKM49_01110"
     CDS             11910..12878
                     /locus_tag="PKM49_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81444.1"
                     /translation="MISFIIRRFLIMIPMMILISMICFFIIELQPGDYVSTLLENPRI
                     SGNMINELRAQLGLDKPGVVRYWLWIKGIVTKGDFGYSFAHNRPVGDLIWERMGWTVV
                     LALTTIVFQWAIGIPLGIFSARHPYSFMDYLLTFLAFLGLSIPEFFLALILIYWMLSM
                     GASSIGGLFSAQYVGAPWSFAKIIDLFQHIWLPIVVVGIGGFGGLMRVMRGNTLDTLG
                     SPFVSALRARGLSEKTVRKHAIKNALNPMVSIAGMELPNVFSGTIIVAIVLGVPTMGP
                     FFYDALLSHDDYLIMAFLMFIAFITQIGNLLADFVLAFIDPRIRMS"
     gene            12890..13981
                     /locus_tag="PKM49_01115"
     CDS             12890..13981
                     /locus_tag="PKM49_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81445.1"
                     /translation="MRDSQTSARVRRAFFKHKLGMVGLIILAILYLSILFADTISPYN
                     YLETHRNFEHVPPSRIYWKHEGEWKGPFVYGVKKVRDPETLAVSYQEDRTKIYPIYWF
                     TRGEAYRFLGLWETDFHLFGLGKEAKGFLFLFGADRFGRDVFSRILQGGRVSLTAGVI
                     GTLISVVIGAIVGAISGYFGGWIDTLIQRFIEVLRSFPRIPLWLTLALILPPGWPSTW
                     VYLGIVVVLAFIGWMGVARVVRGMTLSLREKEFVIASRIVGQSNFKIVTKHLLPNMMT
                     YLVVVATLSIPGMILGESAISFLGLGIKEPMTSWGLLLKQAQSLSEIGAHPWLMLPGI
                     FIMISVLSFNFVGDALRDALDPYRTVEKV"
     gene            13978..14946
                     /locus_tag="PKM49_01120"
     CDS             13978..14946
                     /locus_tag="PKM49_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017812560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81446.1"
                     /translation="MIVVKGLHSYFKTEDGLVKAVDDITFELRPNEVLALVGESGCGK
                     SVTALTVMGLLNRKKTVLKGEILFREKRLLELSPEAYRQIRGDKIGMIFQEPMSAFDP
                     LYTIGYQMQEVLKAHTNLSVEDQKQRAVHWLKNIGIPEAEKRFGQYPHEMSGGMLQRV
                     MIAMALMLQPEVLIADEPTTALDVTIQAQVLGLIRNLQKNEGTSVLFITHDLGVVAEI
                     ADRVHVMYAGKIVEKGTVTRLFHRPLHPYTIGLLTSRVVKGKKGKELPFIPGYVPRAF
                     ELPEGCRFHPRCAQKLPRCERESPPEFLIDHENRVACWLFERGESA"
     gene            14943..15929
                     /locus_tag="PKM49_01125"
     CDS             14943..15929
                     /locus_tag="PKM49_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81447.1"
                     /translation="MILKVDKIKKYFPIKKGIFLLTAGHVRALEEVSFEINEGQTIGV
                     VGESGCGKTTLGRVIAKVYDATSGKLFYNPPKEESVEIRWNTDKRILKNYRKDVQMIF
                     QDPYNSLDPRMTIGDTLAEPLQVHRVCSSKKELNEMVSRLLLKVGLYPEYASRYPHEF
                     SGGQRQRISIARAIALHPRMIICDEPTSALDVSVQSQIINLLKDLQKEFQMAYLFISH
                     NLDVVYHVSDWIMVMYLGNVVEIATPEELFENPLHPYTSALMKAIPNWNPEERRLGET
                     TLQGEPPSPIAPPPGCPFHTRCEFVMERCKVEKPLLTNISENRKVACHLRVP"
     gene            16003..17124
                     /locus_tag="PKM49_01130"
     CDS             16003..17124
                     /locus_tag="PKM49_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNY81448.1"
                     /translation="MEVFFVGLSAITFKQIIMIIVGGVLIYLAIKKDYEPALLLPIGF
                     GAILVNIPFTSVLTQVTDGVTQHGALSILYEAGILTELFPLLIFIAIGAMIDFTPLLQ
                     NPRLLLFGAAAQFGIFFTMTVASLFGFSLKEAASIGVIGTADGPTSIYVASRFAKNLL
                     GPISVAAYSYMSLVPIIQPPVIRLLTTKKERRIRMDYHEKKIPKLVKILFPIIVTLIA
                     GLIAPVSLALIGFLMFGNLIRECGVLERLSNAAQKELANIVTLLLGITIGSTMTAEKF
                     LRVDTLLILGMGLVAFIFDTAGGVLFAKFLNLFLKHKINPMVGAAGISAFPMSARVVQ
                     KMGQQEDPSNFLLMHAASANFAGQIGSVIAGGLILALVK"
     gene            17138..17314
                     /locus_tag="PKM49_01135"
     CDS             17138..17314
                     /locus_tag="PKM49_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81449.1"
                     /translation="MEGMRSYGIFSKEAFETFIQALDVTWKGMAGIFIVMSLLFVAIY
                     LLGKIKHQDQDPSA"
     gene            17376..18818
                     /locus_tag="PKM49_01140"
     CDS             17376..18818
                     /locus_tag="PKM49_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HNY81450.1"
                     /translation="MRYEEIEPRAIFRNFYEINQIPRCSGHEKAVSDHIKNWAIALGL
                     SVKQDHVHNLIIRKPGTLGYEQSAPIILQGHLDMVCDKTPDSTHCFDKDPISLIVEGD
                     RVKADRTTLGADNGIAVAIAMAFLETREIPHPPLEVLLTTNEETGMDGARAVQRKDLT
                     GNTLINLDGEKEGVFLVGCSGGVRVDVRRAITREEASGQFYRLSVGGLKGGHSGADIH
                     LERGNANRILASALCSIKKSVAFRLVDFTGGSKTNAIPRDAKALIVFDPALLETVQRV
                     LDKSFKEIKNEFRTSDPGLWVSGEPVTPQQTAWSVEETDGFLRVMTALPNGLLHRSAD
                     IPGLTETSCNLGVVSTEEAAVTCHASVRSSVTSRKSKVVDQFLAIGALSDSQIITHSD
                     YPPWEYRPQSRIRDIFVESYTFLYGKDPIITAIHAGLETGLFTETLGELDMISIGPNL
                     YNAHSPDESVSISSVARTWELLKEVLRRLK"
     gene            complement(19078..20415)
                     /locus_tag="PKM49_01145"
     CDS             complement(19078..20415)
                     /locus_tag="PKM49_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81451.1"
                     /translation="MSRKLLYVLPTCFYLGFIGGRQLSQWVVFGLILLCALFPKKTVW
                     ALLIGVPPSLYFIHVTGALSLPYLVGFGFVCLLMLSLRRSDVGPKVLLSYCLAWFFLL
                     AVIGGPYATVACVVFLFWLSLKLKKPWLILLIALAFVPATTLWPLSTIPRSSVNEVRS
                     QAQTEEVSAYGQESEDSYGGTAKGNRQEVVTEWVMDQSMPLPLTRFFQWAFIGVGALF
                     ILAAVKFVRDYGKGRLALAKKAGIATLVLGSLFLLTYVLARIPPSGEPLIAEAQAFLE
                     EQGQIGRYQAPSRGSGTLEEKIAPLIRTIEVTMTPINILAIAGTLTLLSFLLIRFIRR
                     KPYKKEQEVVVNIPDIWTDETAAPDLNLDGSELVKAVYRWIRAKRYPAFAMLTPYELL
                     AFHRSESLRRITDAFVRTEYGFSEAGISDDEIRELYHATRNEALLGNTWERGL"
     gene            complement(20405..21682)
                     /locus_tag="PKM49_01150"
     CDS             complement(20405..21682)
                     /locus_tag="PKM49_01150"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNY81452.1"
                     /translation="MEASRTSKTRVTCESKALRLLTIFILAMNLVLFSPYVLIADALV
                     AMVWADLVQKSAVLKRMAVKRSLSATRLFVQERIVVTYRFANNTGVGIDVVCNPSTDL
                     PNLSKREIKLRLNPKAEVMEEVSGVYYTLGQKRVGPVSLFYEGLFGLFGIWKTCEYDE
                     EILVFPYFEEVVFEKEVLRQLLPGRNTAYRLLEDATKVKTTREYVDEPLNRMHWKLSA
                     RYGKLMTKDFDTTALGRVHLVVDMNMPEGVVVNDAWRHMRLRYEEQVVNAAGSVLKEL
                     KKRGTSVRLSIIGEQVWESEFPRTEFVMYLESLVRAKGVENPMVRTTEVLAAYAPQAS
                     LDETILLMTMHLTEREIPLLVKLRARCAKVIVWLMPYGYRPFSYHRSRSFEMPHPEAA
                     KVLEYSKILEENRIIVKFFLDNDALQEVIDRVS"
     gene            complement(21669..22586)
                     /locus_tag="PKM49_01155"
     CDS             complement(21669..22586)
                     /locus_tag="PKM49_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNY81453.1"
                     /translation="MIQNISRVIIGKESVIRQVLGAFFCHGHVLLEDVPGVGKTVLAR
                     ALAVTLGLKTRRIQFTPDLLPTDITGLNIYDKSKKEFVFKEGPVFTQILLGDEINRAT
                     PRTQSALLEAMGERQVTIDGESWRLPDYFFTIATQNPIEYEGTFSLPEAQLDRFLIRI
                     KMGYPDGDSERRLLLDRHSRNPLSEIEPVVDEKELKALFDEVPKVRISEAVLRYIVAL
                     VQKTREHPSLALGASPRGSLALMHLSMVMARIDGRDFVLPDDVKALVIPTLAHRLIQN
                     TESRIKRERVERILEEIVEKTEVVTDGGV"
     gene            complement(23182..>23857)
                     /locus_tag="PKM49_01160"
     CDS             complement(23182..>23857)
                     /locus_tag="PKM49_01160"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY81454.1"
                     /translation="REKWEAPQWFSNRFSNSDRMDGDLKDYIPDYKYELFEISSLRDE
                     EVKGAKRLRIYLDVLRLRALREKAAIREAMFRVAVTISELSRTEANERFFQVCTIYLF
                     ETMGKENFEQLSELMETVSEERSEKMQTIADMLRQEGMEKGMEKGLEMGIIKGREEGR
                     EEGLEKGMEKGREELLWKLISKKFPKASKKYFEKLKTLTIEKLDALGLELIDMKNEEE
                     LKKHLM"
BASE COUNT         5673 a         6323 c         5981 g         5780 t
ORIGIN      
        1 tctccgtgcc ctctgtgccc tccgtgcgaa ccctgttttt ccagacatat acaaaagcgt
       61 atcgcgtcgt cgcaaaaaga agaagcgctt acgccccgat gctctcgtgt gttatcgcga
      121 agtaacccgc gggctgactt cggaagccca cgaagtctcg cgatacgccc tgggaacgcc
      181 tgggaacgcc tgggaacgcc acactttagt gtggcatagg ttcactgttc tctccgtgcc
      241 ctccgtgcga accctgtttt attttcaaga cttgccgact ggaagtcggc gcccacgaat
      301 tttcttcaat cttttctctg tgccctccgt gtgaaccctg ttttattttc aagacatgcc
      361 aacttaaaag ttagcgttct cattttccta cgctgcgtta tgatgctcat tccaataacc
      421 gcgaacgcgc cgaggttgtg atacaataga attcaggagt ccgaaagggc taggcaaccc
      481 ccggccggtt cgttaaatga ccctttcaac tctgcgtggc gtcataagct cacagggtcg
      541 gaagttcaaa aaccgaagcg gcggtcgccc ttcggaaatc ccaaaatata acgactaagc
      601 gaggacaacc gttaaccata gcgcctgaag agaggattca tatggaagat aactcctttt
      661 gcctactggt aaccgatttg aatggccgtg tgaaatatgc aaattccaag gcctgtcagc
      721 gtatgggctt ctttgaaggc gcaaacgagt cgttacttca agaacgactt atttcgactc
      781 tactccctga cgattatttt ttcggctctt tggaagagtt cgctcgaacc aaaggatggg
      841 agcgaaagat cagaaaggtt catcgggatg gaacgattcg atgggagtcg ataacagtgg
      901 tcccggtctt cgacgcgcaa gatgagttcg cgtacgcggt tgtcctgata gaggatattt
      961 ccgatcagaa agggatggcg gatgccctcg ccgtcagcga gaaacggttt cgtgaagtgg
     1021 ccgagcacag ccacaccgtg gtgtgggaaa cggacaccga aggacttttt acctacgtga
     1081 gtccggtttg cgaaagggtt tggggctatc gacccgaaga gatggttcac aagatgcatt
     1141 tttacgacct tttcccacca gacgaacgag aagaggcgaa aaacgagggc ctgtccctta
     1201 tgagagaaga gaaagcgctc acgaatttcg aaaaccgctc gatgcataaa gacggacggc
     1261 tcatttggct ttccagcaac gccgtcccgg tgtatgactc cgaggggctc tacatcggct
     1321 atcggggaac agaaaacgaa atcacggaga ggaagactct cgaggaagag acacaccggt
     1381 ttagggcgac tacggaccag gcgaactacg gcaccgccat cgctcagatg gacggaaccc
     1441 tgaattacgt gaaccacgcg ctcgtgaaga tgttaggaac cgatgaacac gcccttttgg
     1501 gtaaaaatct ctttgcctcg catatcaagg aacgctcgga aagaatcgcc gctttggaag
     1561 aaaagatccg tattcagggc ggattctcct ccgaagaggt gcagttcgtc cgaacggatg
     1621 gcacctcctt tcctgccttg atgagtgtga aggtcgttgt cgacgcgcgc agaagacctc
     1681 tgtttcagtc tctgacgttg atggatattt cgaagctcaa agaatacgaa aaacttctga
     1741 cgctgctgag ccacgccgtc gagcaaaacc ccgcttacgt tatcatcacg gatacgagcg
     1801 ggcatatcga atacgtgaac ccagccttca ccgaaaagat cgggtacgca ctcgaggacg
     1861 tcaagggcag tcgcgcctac atcttgagac cggaacacca ctccccaacg caatatagag
     1921 agctgtggga gaccctggaa agcggaggta cctggcgtgg cgagtttcgg aataaacgaa
     1981 agaacggaga ggtcttttgg gaatactcga cgatctctcc cgtcgtagac caaaaggggg
     2041 aagcaacgca ttacgtcgtt gtgaatacgg acatcacaga acaaaaagtc gcgctcaacg
     2101 cctataaaga aagcgagacc atcctgcaag ccttcactcg aacctcacgg gaagcgattt
     2161 ttatgatcga ttccgaagac acggtgtgct tctggaatcc ctcggcctat gaaatcttcg
     2221 gttatcgaga agaagaggtt ctgggagaaa aactcgattc gttcattcgc ccgtatgttt
     2281 catccgtttc ggaaacgccc tctttcctcg ctctactcaa ggacaatcaa aaagcgcccg
     2341 gcgctgccct cctcgatttg gaggtttaca cgaaaagcgg cgcgctcgtt tccgttgagt
     2401 tttctcaatc cataactttt ctgggagaaa accgctatcg cgtggtggtc ggccgagacg
     2461 ttcgggaacg gaaacaagcg gaagccgacc gtatcgccag agaggccgcg gaggcttcca
     2521 atcgggcaaa gagcgccttt ctctcgaata tgagtcacga aatcagaacc cctctgaacg
     2581 ccatcctcgg gttcgcgcaa attcttaacc gagacgcctc tttgtcgtcg aaacagatcg
     2641 accacgttca aacgattctc cgtagtggag aacatcttct gaaattgatc aacaacgttc
     2701 tggaactctc aaagatcgag gcaggtaaac tcacgctctc cgccgccccc ttcgatctcc
     2761 acaactgtat tttggatttg gagcgtgtct ttcgattcaa agccgaagaa aaaggattac
     2821 aactgctggt ggaacgcgct ccggaccttc ctcattacat cctctccgat gagggaaagt
     2881 tccgccagat tttgatcaac ctgctcggaa acgcagtcaa gttcacacaa acgggaggaa
     2941 tcgccttaag ggctcgcacg gtcccggacc cgacccgcac cgtcgccaaa ccggagagcc
     3001 ttttgctgca ggtggaggtg gaggactctg gaatcggcat ttccgaaaaa gccctatcgc
     3061 gcatctttga agccttcaat acctcagtcg agaactcgtc ggccagtgga accggcttgg
     3121 gtttggcgat cagtaagcgg ttgatcgaat tgatgggagg aacgctttcc gtgaagagta
     3181 aagtcggaac gggttcgcag tttcgatttg aaattccgat tacgccagca gaaggaagtt
     3241 tttataaaag cgcgacgccc accgcagaga ttaaggcgct tcatccaagt tccaaacccg
     3301 ttcgtgtctt gatcgtcgac gatctgacgg ataaccggac actgttacgg gctctgttgg
     3361 aacccatcgg cttcgaaacc gaggatgcgg tcgacggaaa ggaagcgatc gaactcttcg
     3421 agcgatggtc tccgcacgcc attttgatgg acatgcgtat gcccgttatg gacggttatg
     3481 aggcaacccg gctgattaag ggaacggaaa aaggctcaaa aacggctgtt atcgcagtga
     3541 cggccagcgc gttcgacgaa gatgagaagg ccgtgctcgc gaccggcgcg gacggatata
     3601 tacgaaaacc ctttcgaccg gacgagctgc tttcagaact cggcagattg ctgccaatcg
     3661 tctatcttta cgaaagcccg gaagaggaaa ctcccaaaaa aaccgctggc gtcgcaaggc
     3721 tgacgaaaga agacctcgcc atcctacccg aacccttgag gcaacggctc ttccgctatg
     3781 tggaagaagg ggagatgacg gaattgaaaa acaccctcgc caaagtggag gctctaaacc
     3841 ccgaggtctc ccaagccctg ttggcgttgg caaagcaata cgattacgag aggttgaccg
     3901 accttctcaa ggacacttag ataccgcgcc gtttattagg gttttcacct ccgaacaccc
     3961 tgaatttttt ctaaagacct tctttttttg tccagataat catcgcagcg taagcgctaa
     4021 gcgcgaagat gtatcgcgag acttcgtggg ctgggaacgt ctgggaacgc caacttttaa
     4081 gttggcagac cttaaaaata aaacagggtt cgcacggagg gcacggagaa cacagagaaa
     4141 agactgaaga aaactcgcgg ggccgacttt cagtcggcct atctctctgg gaaggccctc
     4201 ggaacgcctg ggaacgccaa cttttaagtt ggcagacctt aaaaataaaa cagggttcgc
     4261 acggagggca cggagaacac agagaaaaga ctgaagaaaa ctcgtgggac cgacttcaag
     4321 tcgacctgtc tctctgggaa ggccctcgga acgcctggga acgcctggga acgccaactt
     4381 ttaagttggc atgttccaaa gaaagctcac acggagacca cgaaggtcac aaagaagaga
     4441 ggtatttagg agagttaaca ccaggtttat gtttcctctg tgctctctgc gtactctgtg
     4501 cgaaacacct ttgttttttt gaaaaaacat gccgaactaa agttcggcgt tcccaaccag
     4561 ctctcccaca gaaccttcta tcatgctata ataaccacat atttctccga agttttctta
     4621 actgagccat cggacttttg gccttacgag gatgcagatt cattaccagc tccagatcga
     4681 gttacaatcg aaggaattgt tcaatgagcg gatttttttc tgctttactt gagaaacaac
     4741 atcagcgatt cagcatcgaa ggagtgtggg atatgaagcg gaaaattctg ttactgacat
     4801 taatatgcgc catcatcgtc ttttcgatcc ttttttctgt cctcggttgt acgacacaac
     4861 ccggccccgg cggttctttc tccgtaatgg tttttaacga gcacgacagt cataccttag
     4921 cgataagaat aaacggtgaa cattcgggcg agtcgaaaaa agggcaacct tattcccagg
     4981 gtggtttctc cgccgccgac cgcgtgacga ttttcaacac ccgtaccggt gtttatctca
     5041 aagatgaact cacggggcaa aacagctgga cgatacacgg taatcttttt ttcacgtact
     5101 ggtatggccc gtacatcgat tttgtttata ctatagacga acattagaac tgcctttgat
     5161 tcgcaaaccc cgtctttgaa ggcagctctc catgcgcgac aagttatgca gtgcaatcaa
     5221 gaaacaaggc cagaatcgat ggcgcgatgc gcttgggaac gccaactttt aagttggcag
     5281 accttaaaaa taaaacaggg ttcgcacgga gggcacagag ggcacgaaga gctcaaaaga
     5341 aaaactggca agggacagcg ttctctcggg agaagaaaag acatgccaca ctaaagtgtg
     5401 gcgttcccag gctttcctag ggtggagcgc gagacttcgt gatcttccga agtcagcccg
     5461 ctggttgcct tacactaacc tattgttctc tgtgcgaaac acctttattt tttttaaaaa
     5521 tctgccgaac taaagttcgg cgttcccgac cagctctccc gcagaactgt ctattatgct
     5581 ataataacca catatctctc aggagttttt caaatccagc tttcggatct ttagccttac
     5641 gaggttgcag attcattacc agctccagat cgagttacaa tcgaaggaat tgttcaacga
     5701 gcggattttt ttctgctctg cttgagaaac aacatcagcg attcagcatc gaaggagtgt
     5761 gggatatgaa gcggaaaatt ctgttactga cgttattatg cgccatcatc gtcttttcga
     5821 tcctttcttc ggctctcagt tgtacgatac aacccggccc tggcggtttt tccgtcacgg
     5881 ttcgtaacca gcacacctgg caaactctat ggttttacag gaacggtaca aggattgcca
     5941 ttatcaatcc tatgacaaac tatgtacgaa acgatttcat agcctccgac attgtacgaa
     6001 tttggaacgc cgatgccagt gttttcctcc atgatatcga cttaaatgac agctggttgg
     6061 taaacggtca tcttaccttc acgtacacga acacgagcgg cggggtaatc catgttactg
     6121 ctgtcttgga cgacgaacgt tagaactatt ttaattcgtg aaccccgcct ttgaaggctt
     6181 tttgattcga tcgatctatg gtataatcct ctcaatatag caaacaggga acccaaaagg
     6241 acggattcat gggcaaaaga atcttgctgg gctgtgcggt tgtatacatt tccatttgtt
     6301 acccacagat agcattctgt gcgagttaca gtgagcacta tatctgactg agcacaaccc
     6361 tcaaaaaatc cgatgccttg ggtttgtttc aaggagaatc tctgttgcag ggcttgtcgt
     6421 tggactttga ggcgacgtat ggaccgccgt ttatgaaacc gtttcgattt cgcgccggag
     6481 tgggttttta tgagctcacc aagatctgcc tgctcgctgg tatggaaatc ccgatattcg
     6541 agcgaatgaa cgattttcgt gcgaaacttt ggggggtcta cctgatcccc gaagtcgctc
     6601 tcggcttttc gcgttgggat gcgagcttac ggtgcgaagt tttgataccg gtgaacgtat
     6661 tgggagggat acagatcggt ctgggagtca acaaagacct caaattgctg gtttctctgt
     6721 cctactccac ggggttgtac ccgttggtca agtgatcgat ccaaaatacc ataagggggt
     6781 aaaaacatga aaaagctggt tctattgttg tcgttagtcg tggttgcgct gcttatgagc
     6841 agctgtacct attttttcta ttctcaggac ggccatttgg ggtactatcc tggggcagtg
     6901 ggggttcccg tgggttcctt ttccgcttcg agggaacaat ggtacttact atcgccgctt
     6961 tggttcccgt tctcggagcc gaacaagcat ctggacgaga tgatcatccc cgaagtcgtc
     7021 aaatacaacg gctctttcgc caaagacctc aagatcggtt acggcttcac cttcatcgac
     7081 tatctgatca cctacttcac cggcggtgtg cttggaagga acaccatcac cgtcgacgga
     7141 caggtcttcc ggaattttta gaaacatcgg aatcggtgcg tgagggcaga acggagcggc
     7201 cctcggacgg tatcgtcacc tcggcgttgc acaaatcgta agtttttggt atataatctt
     7261 cacgtataag tctcttttcc gcctaccttt gctattactc tttggaggag gtcattatga
     7321 aaagggtcgt tataggggct gtgttggttt tgtgtgtgac catcgcgttc tctcagtttt
     7381 ctctcggcgt gatggtcggc caaccctctg ggctttcggc acgctggcaa ctttctgaga
     7441 atctgtcttt ggattttgga gcagcttatt cttttctttg gacaaccgga atacatattc
     7501 atgtcgatgc ggtttttttc gaacgcaccc ttctccgtat ttcagacaat gtaattccag
     7561 tttatttcgg ggcgggagcg cgatatttgg gcgcttattg gggtcccatc ggcatcacta
     7621 ccctaagcgc ccgagtgccg attggcgtgc tctacccctt tcacgtgagc ggcaacgtca
     7681 acctcgaggt ctttgccgaa gtcgtgccaa ctgtcatggt gatacccgcc ttcgctttcg
     7741 acgtatcttt cgcgttgggt gcccggtatc gcttttagcg ccgctgttct ataacagagg
     7801 cgcgatgaga cctgtgaagg agtgatacca taaaacgttt gccgattgca tgggttttag
     7861 cgcaagtatc gattatgttc ttcggattcg caactgtcgt tccatccata tttggtgaga
     7921 ccggaggtgc gaccatccac acctatactt tgaataacgc gaacgggata tccgttcggg
     7981 tattggattt tggagccacc atcctgtcga tctcggtccc ggacagaaac ggccaagtag
     8041 tggatatcct ccttggatat tccaccatag aagagtatgt tcttgacaag agttacttcg
     8101 gatcgaccgt tggacgttat tccggcagaa tcgccaacgg tcagttcgcg ctcgacggaa
     8161 tggtctacac ccttccaaaa aacgacgggc aaaacacgct ccacggaggc cttaagggct
     8221 tcaataaaag actctggaag gcagaggtag aagcggaagg accctttcca gccgtccgga
     8281 tgacctacat cagcccgaac ggcgaagaag ggtttcccgg aaggttgaca gtatccgtgc
     8341 gcttcacgtt gaccgacgat aacgagttga tcctggacta cgaagcgacg accgatcggc
     8401 ctacggtagt gaacctgacg aaccacgcct actttaacct ccgcggggaa ggaaacggcg
     8461 atatcttgtc acattcgctt caaatctttt ccgattattt tgcggagatg gacgaaacgc
     8521 tcataccgac aggcgcgttc cacccggtcg acggaaccgc cctggacttt cgagccccgt
     8581 ttctcgtagg cgcgcggttg gatccctgcg atcaatggct ctcctctgcg aaaggatacg
     8641 atcacagttt tttgcttaga gaggagaacg ccggagcctg cgtcagagcc gcgcgattgt
     8701 atgaaccgga gagcggtcgt gcgctcgaag tgtggacgac cgaacccaca attcatctgt
     8761 acacgggtaa cgtactccaa gcagtttcgg gaaaagcggg aaccacttac ggtccctact
     8821 caggtctctg tttagaaacg cagcatttct cggattctcc gaaccgaccg gagtttccac
     8881 tcactcggct cgactctgga gaccgtttcc tatctcgaac gatctaccgg ttcagcatcg
     8941 atccttaagt cggcccgcaa cggcagaaag cgtgccggca gttagacaag cgctattgag
     9001 gaggaagttc gtgcagcatt tagctatttc gaaaatggcc gaagatatct atctcgcgct
     9061 ctcaaagggc gaggtcattc tctcgacccg cgattcatcg ggtagaccta ccggaatgac
     9121 cgtggcctgg ggattcttcg gatgtttgtg gaacgaacca tactttttgg ccgccgcgcg
     9181 cccttatcgc tatacgtggc agggaatccg agacagcagg cgcttcacgg tcaacgtgtt
     9241 gtccgatgcc tacgccgaag ggctacggta cttcggcacc gtgtcgggga gatcggaaga
     9301 caagttcgca aaaggctatt ttcatattaa cgaagcctta cacgactact gtgcgccgat
     9361 tgaagaggcg cgcctgctcc tggattgcac cgtcgtgacc gccaatcaat tgcagccgtt
     9421 ttttatcccg gaagaggcta tccagcggtt ttaccgagag gacaatgggt atcacacgat
     9481 cttctacggc catatcgatc gatggcatcg ttccgaggcg acgggtgaga actgatgtcg
     9541 tactcctttg aaacgctgtt ggtagaagac tgtcattttg acgaaaacat ctttccggat
     9601 ctcccaatga ttatcctgac cagtatggaa aactacacga cctctatcaa agcgatccgg
     9661 cacggtgtcc tgatcaaaaa cgtatcgcgc cgtcgcaaaa agaagaagcg cttacgccac
     9721 gatgactacg tgtgttatcg cggggtaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnngt gggttttacg accccgcgat ccacacggtg tcccgctcag acgcgcatcg
     9901 cgccgtcgca aaaagaagaa gcgcttacgc cgcgatgact acgtgtgtta tcgcggggta
     9961 agtgggtttt acgaccccgc gatccacacg gtgtcccgct cagacgcgca tcgcgcattg
    10021 atcctgatct tatttcttga tggcgcctca taacttgaca cacaccaaca aatcaccttt
    10081 taaaaaggag gagttgtatg aagaaatttg tgtttgtagt ttgtttactc gtcgcgctcg
    10141 tagcctttgc cacagatttt aaggtcgtag acccgatgtt tacacccgaa ccaaaaccta
    10201 acaaaggagg aaccttgacg cttcgtctgt cggggtcccc gcagtcttgg ctgttctatg
    10261 gctctctcga caacaacgcc tacactgttt gttacaacct ttttgaccct ctgatagagg
    10321 ccaatcctgt caccaacgaa ctgggacccg gtctcgcgaa atcttgggaa gtctccgaag
    10381 acggcttgga agtcaccttc catctcagag acgtcaattg gaccgatggg gtcccgttta
    10441 ccgccgacga cgtcatcttc acctttgaaa acttcgtgat gaacaagtac gctcgtggaa
    10501 acagcatcga tcgtttcacc cttcaaggca aaacgatcga atgggtgaag gtgaatgaca
    10561 agacgttggt cgccaaactg ccctatccgt acggcgcgtt cttcttcgtc acctctcacg
    10621 ttctcatcta tccgaaacac gtggtagcgc ctttgatcga tctttccgat ccggattccg
    10681 tgaacaaagc cttaacaacg gacacgcccg tcaacaagat cgtcggaacc ggctctttca
    10741 tcctcagcca atatgtggtg gatcaaaaag tcgtgttgaa aaagaacccc aacggttgga
    10801 aaaaagacca atacggcaat cagctgccct acgtggacga actggtctac ctcatcgtta
    10861 aggacgcgga ggtcaacgtc gcgaagttcc tcgccggaga gatcgactat atgtctctca
    10921 acgccaggga ctattctaac ttcaaaatca aagaacgcga gggtgcgccg atcaaggtgt
    10981 tcaaagcgct cccgacggga aacacgccca gcccggtaca catcgccatc aacttcgaca
    11041 acaaagatcc ggaattgaaa gccttgttca gaactgacgc tttccgattc gcattggaat
    11101 acgcgctcga cagagaccgc atcatcgatc aggtgttcaa cactctcgcc acctacggcg
    11161 actccgtcta tccgacgaat gcgatgtggt actccaagta cgtggacaaa ctccgcagac
    11221 cgtatgacct gaaaaaagcg gccgcgatcc tggatgaact gggtttgacg gataagaaca
    11281 aagatggctg gagagagctt ccgagcggga agattctgga attcaccatc attatgaaca
    11341 acgcttccaa gagcgaacag gatatcgcgc tcatctacca ggaagacctc aagagcatcg
    11401 gcgtcaaact caacacccag ccgttggatt cttccatccg cagtcaaaaa gcgaccggcg
    11461 gggactttga cctttgtatc tgggccttcg gcacccagcc ggacccgcag ctcagaaagg
    11521 cgatctggca gcccggcggc tctttgtact attggcatct ctctttgttc aacaaagaca
    11581 atccggacgc tccctttatg gacgatatga cctcttggga aaccgttctg tggtacgtct
    11641 gggaaaaagc cgccctggag atggacctcg cgagacggca acatctctac tacatctacc
    11701 aggatatcta cgcagaaatg ctgcccgtta tctttgtcgt tcgagagatg gacgtccgcg
    11761 caggaaacaa caagctcggc aatatgtatc aaaccgaatc gggcgccttc gtattcacga
    11821 actatacggt ctttaagaaa taggcgagca cgtctgatag gttaagaagg gggtgtctca
    11881 cacccccttt ttataaacgg gaggtatgaa tgatcagttt catcattcga cggtttttga
    11941 tcatgattcc gatgatgatc ctcatttcca tgatctgttt ctttatcatc gagctccaac
    12001 ccggagacta cgtcagcacg ctcctagaaa accctcgtat ctcgggcaat atgatcaacg
    12061 aattacgggc ccagctgggc ttggataaac ccggtgtcgt ccgctactgg ctttggatca
    12121 aagggattgt cacgaagggg gacttcggtt attccttcgc gcacaaccgt cctgtcggcg
    12181 atttaatttg ggagcggatg gggtggaccg tagtgttggc tctgacgact atcgtcttcc
    12241 agtgggcgat cggcatcccg ctcggtatct tttccgccag acacccctat tcctttatgg
    12301 actacctact gacctttttg gcctttctcg gcctttcgat accagaattc ttcctcgctt
    12361 tgatcctcat ctattggatg ctctcgatgg gggcttcttc aatcggcggc cttttctccg
    12421 cgcagtacgt aggtgctccc tggtcttttg cgaaaataat cgatctcttt caacatatct
    12481 ggcttccgat cgttgtggtg ggtatcggcg gcttcggcgg cttgatgcgc gttatgcgcg
    12541 ggaacacctt ggatacgctc ggatcccctt tcgtttcggc cttgcgcgcc aggggactat
    12601 ccgaaaaaac cgtccggaag cacgccatca agaacgcgct gaacccgatg gtgagcatcg
    12661 ccggaatgga gctgccaaac gtcttcagcg gcaccatcat cgtggcgatc gtgttggggg
    12721 tgccgacgat gggtcccttc ttctatgatg cgctgctgag ccacgacgac tatctgatca
    12781 tggcgttctt gatgttcatc gccttcatta cccagattgg aaacctgtta gccgacttcg
    12841 tcttagcctt catcgatcct cggatacgga tgagttaggg ggcggatgta tgagagactc
    12901 tcaaaccagc gcgcgagtgc gtcgggcctt ctttaaacat aagctcggga tggtcgggct
    12961 catcatcctc gcgattctgt acctttcaat tcttttcgcc gacaccatat cgccctacaa
    13021 ttacttggaa acccatcgca atttcgagca cgtccctccc tcccgcatct actggaaaca
    13081 cgagggggag tggaaagggc cgttcgtgta cggcgtcaag aaagtgcggg acccggagac
    13141 tttggccgtc tcctatcaag aggatcggac gaaaatctat ccgatctact ggttcactcg
    13201 gggagaggcc tatcggttct tggggttatg ggaaacggat ttccaccttt tcggactcgg
    13261 aaaggaagcc aaagggttct tgttcttgtt cggcgccgac cgctttggaa gagacgtctt
    13321 ttcccgaatc ctgcaagggg gccgcgtctc gttaaccgcc ggggtgatcg ggaccctgat
    13381 cagcgtggtg atcggggcaa tagtcggggc gatttccggg tatttcgggg gttggatcga
    13441 taccttgatc caacggttta tcgaagtctt gcgctccttc ccgcgcattc cattgtggtt
    13501 aaccctggcg ctgatcctcc cccccggttg gcccagtacc tgggtttacc tcggtatcgt
    13561 cgtcgtgcta gccttcatcg gatggatggg agtggcgcgc gtcgtgcgcg ggatgacctt
    13621 aagcttacgg gaaaaggaat tcgtcatcgc ctctcgcatc gtcgggcagt cgaactttaa
    13681 aatcgtcaca aagcatctcc tgccgaatat gatgacctac ctcgtcgtcg tcgcgacgct
    13741 ctcgatccct gggatgatcc tcggagaaag cgccatcagc tttttgggct taggcatcaa
    13801 ggaaccgatg accagttggg ggcttctcct gaaacaagcc caatcgctca gcgagatcgg
    13861 agcgcatccg tggttgatgc tccccggaat cttcatcatg atctctgtgc tgtcttttaa
    13921 cttcgtcggg gacgcgctgc gcgacgcgct cgatccctac cggacggtgg aaaaggtatg
    13981 atcgtggtca aaggcctcca ttcctatttc aaaaccgaag acggcctggt gaaggcggtg
    14041 gacgatatca ctttcgaact ccggcccaac gaggttctgg cgctcgtggg cgaatccggc
    14101 tgtggaaaga gcgtaacggc gctcaccgtg atgggactgt tgaatcggaa aaaaactgtc
    14161 ttgaaaggag aaatcctctt cagagaaaag cgccttctgg agctatctcc ggaagcctat
    14221 cgccagatac ggggcgacaa gatcggtatg atctttcagg agccgatgtc cgccttcgat
    14281 ccgctctata ccatcggtta tcaaatgcaa gaagttttga aagcccatac gaacctttcc
    14341 gtcgaagatc aaaaacaacg cgcggtccac tggctgaaaa acatcggtat ccctgaagca
    14401 gagaaacgtt tcggacagta ccctcatgaa atgagcggcg gtatgctcca gcgtgtgatg
    14461 atcgcgatgg cgttgatgct ccaaccggag gttttgatcg cggacgagcc cacgacggct
    14521 ttggacgtga cgatccaagc gcaggtgctg gggttgatcc gtaacttaca gaaaaacgag
    14581 ggcacttccg ttcttttcat cacccacgat ttgggagtcg ttgcggagat cgccgaccgc
    14641 gtgcacgtga tgtacgcggg caagatcgtc gaaaaaggta cggtaacccg gctgttccat
    14701 cgcccgttac acccttatac gatcggacta ctcacctccc gcgtagtgaa gggcaagaaa
    14761 ggaaaggagc tccctttcat ccccggctac gttcctcgcg ccttcgagct accggaaggc
    14821 tgccgcttcc atccgcgttg cgcgcaaaaa ctcccccgtt gcgagcgcga aagcccgccg
    14881 gaattcctta tcgaccacga gaaccgcgtc gcttgctggc tttttgagag gggggaatcg
    14941 gcatgattct caaagttgac aagataaaga aatactttcc tatcaaaaaa gggatattcc
    15001 tcctgaccgc cggacacgtc cgcgcgcttg aggaggtgtc tttcgaaatc aacgaggggc
    15061 agaccatcgg agtcgtcgga gagtccgggt gcgggaagac tacactcggg cgggtgatcg
    15121 caaaagtgta cgacgcaact tcgggaaagc tcttttataa ccctcccaaa gaagagagtg
    15181 tcgaaatacg atggaataca gacaaacgca ttctcaaaaa ctatcgaaaa gacgttcaga
    15241 tgatcttcca ggatccctac aactctttag atccgcggat gaccatcgga gacaccttgg
    15301 ccgaaccgct ccaagtccac cgggtttgct cgtcgaaaaa agaactgaac gaaatggtca
    15361 gtcggctcct cttaaaggtg ggcctttatc ccgagtacgc ttcccgctac ccgcatgagt
    15421 tcagcggcgg ccaacgtcag cgtatttcca tcgcccgggc gattgcgcta catccgcgga
    15481 tgatcatatg cgacgagccc acttccgcgt tggacgtctc ggtgcaaagc caaatcatca
    15541 atttgcttaa agacttgcag aaagaatttc agatggccta tctcttcatc tcacacaatt
    15601 tggacgtcgt ctatcatgtc agcgattgga tcatggtgat gtatctaggg aatgtggtcg
    15661 aaatcgcaac gccggaggag ttgtttgaaa acccgcttca cccttacacc tccgcgttga
    15721 tgaaagcaat tccgaactgg aacccggaag agcgaagact tggcgaaacg accctccaag
    15781 gcgagccgcc gagtccgata gccccgcctc ccggttgtcc gtttcacacc cgttgcgagt
    15841 tcgttatgga acggtgcaaa gtagaaaagc ctcttttaac gaacataagc gaaaatcgaa
    15901 aggtagcgtg ccatttgcgc gtgccataag gtataataac ttcatcgata tctcggcaca
    15961 cgaggtgttt ttcctcaaaa cctaggagag gggggcttcc cgatggaagt gttttttgta
    16021 ggtctaagcg cgatcacttt taagcaaatt atcatgatca ttgtcggagg tgtgttgatc
    16081 tacctcgcga taaaaaaaga ctacgaacct gcccttctgt taccgattgg atttggtgcc
    16141 atcctcgtca acattccatt cacttccgtt ttaacccaag tgaccgatgg tgtcacccaa
    16201 cacggggcgc tttctatttt gtacgaggcg gggattttga ccgaactctt cccgctcctc
    16261 atcttcatcg cgatcggcgc gatgatcgat tttacgcctt tgttgcaaaa cccgcgactt
    16321 ctgcttttcg gcgccgcggc gcaattcggc atctttttta cgatgacggt cgcttcgctt
    16381 ttcggcttca gcttgaagga agcggcttcg atcggcgtta tcggcacggc tgatggcccg
    16441 acctcgatct acgtggcctc ccgtttcgcg aaaaacctat tgggtcctat atccgtagcc
    16501 gcctactcct atatgtcctt agtcccgatc atacaaccgc ctgtgatcag actcttgacg
    16561 acaaaaaagg agcggagaat ccggatggac tatcacgaga aaaagatacc gaagctggtg
    16621 aaaattctct ttcctatcat cgtcacgctc atcgccggcc tcatcgcgcc ggtgtccttg
    16681 gcgctcatcg gatttttgat gtttggcaac ctcatccgag aatgcggcgt tttggagcgg
    16741 ttgtcgaacg cggcgcaaaa ggagttggcc aacatcgtga cactgctttt gggaataacg
    16801 atcggatcga cgatgacggc ggaaaaattc ttgcgcgtgg acacgctttt gattctgggc
    16861 atggggttgg tcgccttcat ctttgacacg gccggaggcg tcctgttcgc caaattcttg
    16921 aacctgtttt tgaaacataa aataaatccg atggtcggcg cggcggggat ctcggccttt
    16981 ccgatgtccg ctcgcgtcgt tcaaaagatg gggcagcagg aagacccctc caacttcctt
    17041 ttaatgcatg cggccagcgc caacttcgcc ggacagattg gcagtgtgat agcgggcggt
    17101 ctcatcttgg cccttgtcaa gtgagagagg tgtgactatg gaaggaatgc gctcatacgg
    17161 aatcttctcg aaagaggctt ttgaaacgtt tatacaagcg ttggacgtga cttggaaggg
    17221 gatggcgggc attttcatcg tgatgtccct gctctttgtg gcgatctatc tcttgggcaa
    17281 aataaagcat caagatcaag acccttccgc ttgaactggt ttcattgact cacgatagat
    17341 acgacaaatc tatagaagga ggtactcctt ttcttatgcg atatgaagaa atagaaccga
    17401 gagcgatatt tcgcaacttc tacgagatca accaaatacc acgctgttcg ggacacgaaa
    17461 aagctgtcag cgatcacatc aaaaactggg ctatcgcctt agggctatcg gtcaagcagg
    17521 accacgtcca caacctaatc attcgaaaac ccgggacctt gggatatgaa cagtctgctc
    17581 ctatcatcct ccaggggcac ttggacatgg tctgtgataa aacgcccgat tcgacccact
    17641 gtttcgataa agacccgatc tctcttatcg tggagggtga tcgagtcaaa gcggatcgaa
    17701 cgaccctggg agccgacaac ggaatcgccg tagcgatcgc gatggctttc ctcgaaacga
    17761 gagaaatccc acatcctcct ttagaagtgc tcctgacgac caacgaagag acggggatgg
    17821 atggcgcgcg ggctgttcaa agaaaagatt tgaccggcaa cacgctcatc aacctcgacg
    17881 gcgaaaagga aggcgtcttt ttggtcggtt gttccggtgg cgttcgtgtg gatgtgagac
    17941 gcgcgatcac ccgcgaagag gcgagtggac agttctacag gctgtctgtc ggaggtttaa
    18001 aaggcggaca ctccggcgcc gacatccacc tcgaacgggg taacgcgaat cggatacttg
    18061 cgtccgctct ctgttccatc aaaaaatccg tcgccttcag acttgtcgac ttcaccggcg
    18121 gatccaaaac caacgcgata ccgcgagatg cgaaagcctt gattgtcttc gaccctgcgt
    18181 tgctcgagac ggttcaacgc gtccttgaca aatcctttaa agagataaaa aacgaattcc
    18241 gaacctccga tccgggccta tgggtatcag gagagcctgt tactccccaa caaacggctt
    18301 ggtcggtcga ggagacagac ggtttcttac gcgtgatgac cgctttgcca aacggattgt
    18361 tgcaccgaag cgcggatatc cccggattaa ccgaaacctc ctgcaatctc ggcgtcgtgt
    18421 ctaccgaaga ggcggcggtg acctgtcacg cttcggttcg cagctccgtc acatcacgaa
    18481 aatcgaaggt tgtcgatcaa ttccttgcca tcggggcctt atccgactcc caaatcatca
    18541 cgcattcgga ctatccccct tgggaatatc gcccccaatc gcgcatccga gatatcttcg
    18601 tcgagagtta caccttcctt tacgggaagg acccgatcat cacagccata cacgcgggac
    18661 tagaaaccgg tttgttcacc gaaaccctcg gcgagttgga tatgatctct ataggaccta
    18721 acctttacaa cgcccactct ccggacgaat cggtgagcat ctcctccgtc gcccgcactt
    18781 gggagttatt aaaagaagta ttgcgccgat tgaaataacc tactctacgg taacggtctg
    18841 tgagcgcctt gggaactccg aactttaagt tcggcatgtt ttaaaagaag atgaagacaa
    18901 gaaaagcgta gcgcgagact tcgtgggctt tcgaagtcag tccgcttttt accacgcgat
    18961 aatctacaca gccatcgcgg cgtaagcgcc ccttcttttt gcgacggcgc gatacgtttt
    19021 tgtacgtgtc ttgaaaaaca gggtgcacac ggagaaaagg ttcttgggaa cgccgcacta
    19081 aagtccgcgt tcccaggtgt tcccaaggag ggcttcgttt cgcgtcgcgt ggtatagctc
    19141 cctaatctcg tcatccgaaa ttcctgcttc tgagaagcca tactccgtac ggacgaatgc
    19201 gtcggtgatc cgtctgaggc tctctgaccg gtggaaggcg agcagctcgt agggggtcag
    19261 catagcgaaa gccgggtagc gtttcgcgcg tatccatcgg tatacggcct tgaccagttc
    19321 gctaccgtca aggttcaggt ctggcgccgc ggtttcgtct gtccaaatat ctgggatatt
    19381 cacgacgact tcctgctctt ttttgtaagg ctttcgacgg ataaatcgta tcagaaggaa
    19441 agacagcaac gtcaaggtgc cagcgatcgc caagatgttg atcggggtca tcgttacctc
    19501 gatggtgcgg atcaggggtg cgattttttc ttcgagcgtc cctgagcccc tcgagggagc
    19561 ctgataccgg ccgatctgcc cctgctcttc taagaaagcc tgcgcttccg ctatcagcgg
    19621 ttcacccgag ggcggtatac gcgcgagcac gtaagtcaga aggaaaaggc tgccgagaac
    19681 gagcgtcgca atgccggctt ttttcgccaa agccagtcgt ccctttccgt agtctctgac
    19741 gaactttacc gccgcgagga tgaatagcgc cccaacgccg atgaacgccc attgaaagaa
    19801 gcgggtcagc ggaagaggca tcgattggtc catcacccac tcggtcacga cctcttggcg
    19861 attgcctttt gccgtgccgc cgtatgaatc ctccgattcc tgcccgtacg cggatacttc
    19921 ctccgtctgc gcctggctcc gtacctcatt gacactcgac ctcggtatcg tacttaaggg
    19981 ccacagcgtg gtggccggta cgaacgcgag ggcgatcaac aaaataagcc acggtttttt
    20041 caacttcaat gaaagccaaa agagaaagac cacgcacgcg acagtcgcgt agggtccacc
    20101 gatgacagcc agcagaaaaa accaagccag gcagtaggag agtaaaacct tggggccgac
    20161 gtcacttctt cgcaaactca acataagtag acagacgaaa ccgaacccga cgaggtatgg
    20221 cagggaaaga gcacccgtga cgtgtatgaa atagagagac ggaggcacac caatcagaag
    20281 ggcccaaacg gttttctttg gaaacagcgc gcataggaga atcaaaccga acacgaccca
    20341 ctgagagagc tggcgtcctc cgataaatcc cagataaaaa caagtgggta aaacgtacag
    20401 cagtttacga gacacggtcg atcacttctt gtagggcgtc gttatccaaa aagaatttaa
    20461 cgatgatgcg gttctcttcc agaattttcg agtattccaa cactttcgcc gcctccggat
    20521 gcggcatctc aaagcttctc gagcgatgat aggaaaaggg acgatagccg tagggcatca
    20581 gccagacgat cactttcgcg cagcgtgccc gcaatttgac cagtaacggg atttctcgtt
    20641 cggtcaagtg catcgtcatc agcaagatcg tttcgtccaa agacgcttgg ggcgcgtagg
    20701 cggccaatac ttcggtcgtt cgtaccatgg gattttccac ccctttcgca cggacgaggg
    20761 actccaggta catcacaaac tccgttctgg ggaattccga ctcccaaacc tgctcgccga
    20821 tgatgctgag tcgaacagac gttccccgct tcttcaactc tttcagaaca ctccccgcgg
    20881 cgttgaccac ttgctcctca taccgcagac gcatatgcct ccacgcgtcg ttcacgacga
    20941 ccccttccgg catattcata tccacgacca gatggactct gcccaacgcc gtcgtgtcga
    21001 aatccttcgt catcagcttt ccatatcttg cagacagctt ccaatgcata cgattcagag
    21061 gttcgtcgac gtattctctc gtggtcttga ccttcgtggc gtcttctaac aaccggtagg
    21121 cggtgttccg ccccggaagg agctgtcgca agacctcttt ctcgaagacg acctcttcaa
    21181 aataggggaa aactaaaatc tcttcgtcgt attcgcaggt cttccagata ccgaagagcc
    21241 caaaaaggcc ttcgtaaaag agggagaccg gcccgacgcg cttttgaccc aacgtgtagt
    21301 acactcccga gacttcctcc ataacctccg ctttcgggtt gagccgaagc tttatctcgc
    21361 gcttcgaaag gttcggcaag tcggtggacg gattgcagac cacatcgatc ccgacgccgg
    21421 tattgttagc aaaccggtag gtcaccacta ttctttcttg aacgaacaac cgagtagccg
    21481 agaggctcct tttcacggcc atccttttta agaccgcgct tttttggacg aggtcggccc
    21541 aaaccattgc caccaaggcg tctgcgatca aaacgtacgg gctgaacaag accaggttca
    21601 tcgctagtat aaagatcgtt aaaagcctta gggctttcga ctcgcaggta acgcgcgttt
    21661 ttgaagttct agacgcctcc atccgtgacg acctcggttt tctccacgat ttcttccaaa
    21721 atacgttcga ccctttctcg cttgatcctc gactcggtgt tctgtatcaa acggtgcgcg
    21781 agcgtgggga tgaccagcgc cttcacatca tcgggaagga cgaaatcgcg gccatctatg
    21841 cgggccatca ccatcgatag gtgcataagc gcgagcgacc ccctcggact cgctccaagc
    21901 gcgagcgacg gatgctctct cgtcttttga actaaggcga cgatgtaccg cagcaccgct
    21961 tcgctgatac gaacctttgg aacctcgtcg aacagcgctt tcagctcctt ttcgtcgacg
    22021 acgggctcga tctcggacaa aggattgcga ctgtgtctgt ccaggagcaa cctccgctcg
    22081 ctatcgccat ccgggtaacc cattttgatc cgtatgagaa aacggtcgag ctgagcctcc
    22141 ggtagcgaaa aggttccctc gtattcgatt gggttctgtg tggcgatcgt aaaaaaataa
    22201 tccggcaagc gccaactctc tccgtctatc gtcacctgtc gctcccccat cgcttcgagt
    22261 aaagccgatt gggttcgcgg ggtcgctcgg ttgatctcgt cgccgagcag aatctgcgtg
    22321 aacacgggcc cttctttgaa gacgaactcc tttttggact tatcgtagat atttaatccg
    22381 gtgatatccg tgggcagcaa gtcgggtgta aactggattc tccgggtttt caaacccaaa
    22441 gtcaccgcca gcgcgcgcgc caagaccgtt tttcctacac ccggaacgtc ctcgagcaac
    22501 acgtggccgt gacaaaagaa ggcgccgagc acctgccgga tcacagactc tttcccgata
    22561 atcacccgcg atatgttttg aatcatcttc tgaatcttcg cttcgaacac ggacctactc
    22621 ctccgccgca cctttggcgg ctcttttatt ctccgttttt ttcgacgggt tttcgtcttt
    22681 ttgccctgtc gcctactcgt cgaaaaccct tcattcgtcg aaaatttcgt tcctcgctta
    22741 aacaggaccg gatcgtttcc tacgttttgc cggcttttct ggcgactcat atcgcctttc
    22801 gacgggcttt tcgacaatag ttcggttata gaatcgtttt cgacggagtc ttttttctat
    22861 aactataccg tcttacacgc gttttcgacg ggttttcgac gcttataatt attatttcta
    22921 aaagccttta tagagaagag gatatactcg gtgtcctccg tttctcactc tactcttctg
    22981 gttttctctg tgccctccgt gtaaaccctg ttttatttct aagactcgcc aacttaacag
    23041 ttggcgttac cgtgtggata ttatacgaga cgcgcaaaaa agacaagagg aggatgagga
    23101 aacaaagcgg ccccctaagg agccgccata atttcgcgca cacgatctat caaatcgtgt
    23161 tcaagccctt tgtcggactc tctacattaa atgcttcttt aactcttcct cgtttttcat
    23221 atcgattagc tcaagtccca gagcgtcaag tttctctatt gtaagggtct tcagcttttc
    23281 gaaatacttt ttcgaggcct tagggaactt tttggatatt agtttccaaa gcaattcttc
    23341 tcttcccttt tccattcctt tttctaaccc ttcttctcta ccttcttctc tgccttttat
    23401 aatccccatt tctaagcctt tttccattcc cttttccatt ccttcttgtc tgagcatatc
    23461 cgcgattgtt tgcatctttt cgctcctttc ttccgatacc gtttccatca gttctgacaa
    23521 ttgttcgaaa ttctccttgc ccatcgtttc aaacaggtag atcgtacaga cctggaaaaa
    23581 ccgttcattc gcttctgttc gcgacaattc cgaaatcgtc actgcgactc ggaacatcgc
    23641 ttctcggatc gctgcttttt ccctgagcgc tcttagcctt aggacgtcca aatagattcg
    23701 aagccgtttc gcccctttga cctcttcgtc tctcaatgac gaaatttcaa acagttcata
    23761 tttatagtct gggatgtagt ctttcaagtc cccgtccata cggtcgctgt ttgaaaagcg
    23821 attcgaaaac cattgcggcg cttcccattt ctccctc
//
LOCUS       DAOPXN010000010        22614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2285_length_22614_cov_5.271244, whole genome shotgun sequence.
ACCESSION   DAOPXN010000010 DAOPXN010000000
VERSION     DAOPXN010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 22614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22614
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2285_length_22614_cov_5.271244"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKM49_01165"
     CDS             <1..1009
                     /locus_tag="PKM49_01165"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNY81455.1"
                     /translation="PGRIRPKTLLWLVICITFLVGSGFGYSFLFPNLVYYERGNLLEL
                     STSFEGFSVLMSPRFLMPTVWTYRVQDPVIAALIKTESDFILHNRSSSNALGIYQMKP
                     FFASSLGIWNPFFPWNEQKVSGYLEDLQKKYGSLRAALTIYHRGEAGYLSMKKDSNAS
                     DKLYNYAEKIEGFQRQYSKGQMVRLKDFWRIGLGFSSSWKGGNSGRLTVVAPTAWFGT
                     SAAVLTVEDAVRIELCQEFMISSQFQVFVGYRESAVIGCSFRSQTWNDVLAIRYDFGT
                     RELSWEGKFEKSPLFFSLSGSVEAVTVRCGFIFDNRYQVTLGLKCGASLAPFFSAKAV
                     F"
     gene            1063..1530
                     /locus_tag="PKM49_01170"
     CDS             1063..1530
                     /locus_tag="PKM49_01170"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNY81456.1"
                     /translation="MERLKEVWIVKETEKEPWIIIEKSKPASCESCGSMSFCRVKNVV
                     ETRAKNETGKTLKPGQHVLVEVPLVSSTKTAFFLYGIPTLALVVSLLLFIEGFGLSQT
                     QGLLYALLVLAGVFLALWFYDKAYAKRETYTARILAVLPEMKSVDRPEGRDAL"
     gene            1589..3910
                     /locus_tag="PKM49_01175"
     CDS             1589..3910
                     /locus_tag="PKM49_01175"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HNY81457.1"
                     /translation="MKKPFLSTMVLFLLLLILPRVGLAIQIGFLEITGNVRVTREEIV
                     ANLVKCKEGYSVTLADIDNDIFRLRDMGIFQDVKYQLTKYIGDTMTLTFEVLEYPYVK
                     EVKVRVEGPDLVDTKDMKQAILVEANQVLNYKKLFRTISAIKTFFMNSGYHTVEVTDN
                     LQKTDGGVFLPDQTLILTIKEYGLYDVILKGELGDITYSEVRELLGVRTFKDYYEDFW
                     RWLRIKSKYYPSISDYQMGLSRLFSTGLIGPKTQYDFQVLKEPLKNGEHVVNLILTIE
                     LNPVVPRDKEVQTILFTGNTLVSSDKLREGLRSLRNPKTVLIDVLRDAQKIKKTYEEE
                     GYPFVIVTPSYQEQSKALVFTVTEAVISDIRLEGLTKTQEYLVWKEIRLKRGEPIKMR
                     NLKQTYINLNRTGYFESVDIEPLGFSAGSTPTVLVIHLAEAWNNLAVNAGVSYDPRNE
                     GSTFAQKVYGTISLALTNPFGYGQTIDFSATLGKYPALKFGYKIASIFGSHFDAGVDL
                     SYQDQYTSRRVSELSTTVTYEEEKYTIMPELTFHITDSQRITGSFSWGKFSRKDFSID
                     EATATKYIEPKGDIWTLSLMYTFDTRDDLGDPMEGINAFVRGDFSLPFATDPWLRFQE
                     NFSIFFSPFDYHHTLGGRLLFSEEAWDPHGVLSYTLGGANNFFVRGIPYDRASTTDHF
                     GVLNLEYRFRLSKQGEMGVSFAAFLDQALAFNDLSSVSIDNWLVALGGGIRLTIPGFG
                     ILRLDIGWDFSPTLWDEGGPQWGGFHFGFGQMF"
     gene            4005..5114
                     /locus_tag="PKM49_01180"
     CDS             4005..5114
                     /locus_tag="PKM49_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81458.1"
                     /translation="MQGISGALKEIGQIENLSKTYEMDLREAIWKREIRRFCQQHNLY
                     LPVAETFFTKNWVLAWYDAQGTAEPFSVDRIQDLSWTLHILRDALRNGVVAFEDFPGA
                     SEWLSEVYGGPLASVKNGETRFSSAYPNLLQMAMEKVIYREDFSKSIVFFQELPECIV
                     EVKNAYKFLQTETKTEKAYHAQYARFSEKEALAAIDAAMFLEEIERVTLTNVLSFKEN
                     IRRWFILPPEDKALVRFLDALSDKTALVLFQSGEADWVLTLFLSVPSDTFRELFESWG
                     IGATERPFPRIVPFADALCYFFIEGNRLHFSNRSALPNRELPGAAKEEMRRSVLESKA
                     YEIDYSRTPSKGASLHRLLSRRYENNLLLESERVF"
     gene            5114..5377
                     /locus_tag="PKM49_01185"
     CDS             5114..5377
                     /locus_tag="PKM49_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81459.1"
                     /translation="MRSERFDAEIDMPNHAIQRGYERELTAREIIDAIDHPQARCRLQ
                     HNGRFRIESGPVVVIVQKDLHGFRVVTAFFTKRRDTYGKDSFP"
     gene            5355..8324
                     /locus_tag="PKM49_01190"
     CDS             5355..8324
                     /locus_tag="PKM49_01190"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81460.1"
                     /translation="MAKTLFLSLLLGICSLSFGLSILDITLTLESRLLFETQKSTEEV
                     HLLLPALVPLKAFLILDEEAAESLRIGSSRYLLLAYASGEWQEHSLPALSPVSPVNSH
                     IKAASNYMALLTSFLEADTLVQNVSVYHLLSSKMEYLDIFLDELEQLQLSLYEPFKKQ
                     ALRVAFADYLSLLHEGKKQFFLSLPKFFLRENVEFKVLEFEPAFSDLIEQTISEWNRL
                     MDRVHTPLQFDSFLSGLSVFLEGLALNPKKPIGATVTEWMKRLDSGEVSDVKIRTPYH
                     HPFFSAENTVFADLQTALLLDTQKELAIWIHEEYRKMVLYASRTLGFLTDSHIERILA
                     GVFLFELCSLKTMALIGRYGNETARRKVLDLYNTQYTPGQDSQELFPQTAELIREELV
                     QMMIGISDLGGAAPLFRESAGTTVPALEVRPVTTPKTYSLASAELVHMLELETPLGHF
                     AEAVMSVRVGTRKADTFTKIYPVFPTQTTRGKPLILLDFFWFAIKQWGQTLGKLLGLM
                     LNRTIDTIEMENRLELKGVEVTIPRYYEMRPYAELWRMQPADDQNIFVSVQNNNSEKN
                     AVSNLFGGVIPDFLAGDIDKIPADPIFRIHRYPTEVFTTTKGLRNSAPRGSRALILVH
                     GKQALPNFTREKEVMVHVPYIWRTEPRLAVWDRLYLLWTERARAAETGSVFEAYDFYE
                     FVYDTSVLTAREYGQILAGILQDYGFLHDYETLVIVGHSMGGIVAREAANTRLERPSD
                     PQKAFLGDYLSAIITLDSPHYGSVLENFVYTVNAELKARIKESKQPDDVFRINLYELL
                     RALLRDDYVDQSLQVLLAITAKNPELVGKVFTEMVSFLDPFPGGVCLSYTVDSYTRAV
                     ADYLYGKVDARRYSDILKGPEDLRLLNQNDRFYDRLYLFTSEIDENSELSNSNYSLLY
                     ALMKTTTQKVTFYTNRDFSVHEANDCVVSLYSQQMAGSDKGQTRIAFQGIDHTAVPMD
                     RAVLNRLFQTIEQVSGR"
     gene            8333..9895
                     /locus_tag="PKM49_01195"
     CDS             8333..9895
                     /locus_tag="PKM49_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HNY81461.1"
                     /translation="MTATREKALQAIEGLILYALERDLIAPEEIHYTRNTLLGFLRLE
                     VPLSDVKQRPVEPLPRYLDTLLDYAREHALLEEETQTRRDLLDTAIMGLLTPRPREVA
                     RRFYEHRLIDVTKATDWFYRLCIDTNYIRMDRMQKNIKWRSETEYGALEITINLSKPE
                     KDPLEIAAEKLKPQIGYPRCLLCVENVGFHGNLRHPARQNLRVIPVTLAEEPWFFQYS
                     PYVYYNEHCIVLSEEHRPMKLERKTFARLFDFLDQFPHYFIGSNADLPVVGGSILSHD
                     HFQGGRATMPMEKAPIDRHYTLSRFPGVETGRVRWPMSALRLRSDDRRVLVDASTALL
                     DYWRTYTDASVEVIAQTDAPHNTMTPIARKTGSGQYEIDLVLRNNRSNPQYPLGIFHP
                     HPELHAIKKENIGLIEVMGLAILPGRLRTEMRRVQEWMTGDHHVTLASLDPSDELYKH
                     REWVDPLISEKGVGHSPERARALIEEEIGRKFATVLEHCGVFKRDSNGFAAFDRFVYN
                     FNQIRESTGGLR"
     gene            9892..10581
                     /locus_tag="PKM49_01200"
     CDS             9892..10581
                     /locus_tag="PKM49_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNY81462.1"
                     /translation="MKKMMLGICILCFSIVAFGVIPLKDVSPSHWAYESVQYLIEKGI
                     LTGLPDGTFQGEAYLTRYQFSVAMYKAFQLLERKAFTGEVTSTQDLSTINFQVSTLKG
                     LVETIAAKMERMGRDYQDLVKRIDQMGTNTDLANQASQTAKLLSGLETRVIELEVEND
                     SVIMKLAAQERGLADYRRIVENTGLEYQNLFAQHQKLNQKVNILIGVAAAASVLASVG
                     LGLSIYLIATR"
     gene            complement(10717..11304)
                     /locus_tag="PKM49_01205"
     CDS             complement(10717..11304)
                     /locus_tag="PKM49_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HNY81463.1"
                     /translation="MKIKEGAIIVGVSARHLHLSPAHLEILFGAGATLTKYKDLRQPG
                     QYACDEKVTIVGPKGSFENVRVLGPVRKDTQIEISMTDALKLGVTPPIRDSGDVVGTP
                     GVELIGPKGSVKLDKGLIIAKRHIHMLPEEAQRYGVKDKDLVAVFCDTKGRKAILMDV
                     LIRVNPTYALEFHVDTDESNAAMLKTGDLVHIVEL"
     gene            complement(11349..11840)
                     /locus_tag="PKM49_01210"
     CDS             complement(11349..11840)
                     /locus_tag="PKM49_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNY81464.1"
                     /translation="MEGNDGFSGVEAPSKYKELERSLRRLAAWIKAEGRKVIKEGNYK
                     ITPAQFDVLQRVYWGGDSTMSNLSRRLGVAKSTITGLITKLKEAGYIEYSVLEEDHRM
                     RKVVITPKGKALIQEVIRRRVLFVERLFVTVDPFLTDEFRKVLRIVNNTLDVNEANES
                     RTT"
     gene            complement(11830..12498)
                     /gene="rsmI"
                     /locus_tag="PKM49_01215"
     CDS             complement(11830..12498)
                     /gene="rsmI"
                     /locus_tag="PKM49_01215"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNY81465.1"
                     /translation="MTLVASPIGNLEDFSFRGVRILREADFILAEDTRRSLILINHYQ
                     IGRKAIISFSEQKIERKLDAVLERLRNGENGVLLTDSGMPAVADPGSKLMRACHEHRI
                     PVDVIPGPSAVVSAFACSGFSGSFIFEGFLPRAKRLKRLLATFKEEERAIVFFESPYR
                     IKDTLELIEQILGDRQIFIAKEMTKVHQEFFYGRVSEVKTAFEGEARGEYTIVLGGRE
                     LNGG"
     gene            complement(12545..15187)
                     /locus_tag="PKM49_01220"
     CDS             complement(12545..15187)
                     /locus_tag="PKM49_01220"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNY81466.1"
                     /translation="MEPLSKAYDPQGIESKWYATWKENGYFKPSGKGEPFTIVIPPPN
                     ITGKLHMGHGLNMTLQDIAIRFNRMLGKNTLWLPGEDHAGIATQGVIEKNLREKGTSR
                     DAVGREEFLRITWDWANEYRKHIKNQIEWIGCSVDWERDRFTLDEGLNHAVRRAFVHL
                     YHKGLIYKGKYLVNWCSRCGTVLSDEEVEHEEEKSHLWYILYPLKGEKLGLTVATTRP
                     ETMLGDTAVAVNPSDERYRDWIGKTLILPIVNREIPIIADHFVDPQFGTGAVKVTPAH
                     DPNDYQIALRHNLPYVEVIDERSYMSPETGVFAGLTTLEARKRVVERLESDGFLVKIE
                     DYRHAVGHCYRCSATIEPRLSDQWFVKMKPLAAPAIEVVESGKVKFHPDRWKKVYLNW
                     MSEVRDWCISRQLWWGHRIPVWYCQDCGHPNVTEEAPERCEKCGSSFLIQDDDVLDTW
                     FSSGLWPFSTLGWPDQTSDLESFYPTSLLSTAFDIIFFWVARMIVMGMEFIGDIPFSD
                     VYIHQLVRDKKGRKMSKSLGNGIDPIEVIQAYGADATRFSLAILASQGSDIRYDVKSV
                     ETYQHFANKLWNASRFALMNLDPSNGGASLAIHPDQCTLADGWILSSLNDTIRKTTDF
                     LSRYEYNFAAKSIYEFFWNQFCDWYLESAKPILYGDSGPKQNTRAVLAYVLDQSVRIL
                     HPFMPFITEEIWQHLPVPKEAPSLIIAPWPEERPEYAFVEKRSRYEALIEEIRGIRNL
                     RAQFQVPPSRKLTVAFRFLGESGTALLEEELAYIRFLANVEEVAVVDRKPEKSATAFV
                     DSSREIYVILGETIDWEGERQRLEKEIDKTRVDLQHALSKLNNPKFMAHAEAEAVEEA
                     QLKAEEAKKTLQRLELLLKDFFTA"
     gene            15754..16422
                     /gene="rsmG"
                     /locus_tag="PKM49_01225"
     CDS             15754..16422
                     /gene="rsmG"
                     /locus_tag="PKM49_01225"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNY81467.1"
                     /translation="MLDFSKETEYALDRYIEMIIEENKRQNLIRFTDHEAILTKHVLD
                     SALPWMAHRIELEGKGVDIGSGAGFPGIVIKLCFPNLSLTLYESEKKKAAFLRRVIQQ
                     LGLDGCRVVADRVESAGRPQSYDAVFARAVGGLPTLLEYAAPLLAVGGKAYFYKGPKY
                     PEEIEKSERARRILGWESPEILPYTFTYKGELYRHWLLVYRKDMETPERFPRRLGAAA
                     KEPL"
     gene            complement(16476..18209)
                     /locus_tag="PKM49_01230"
     CDS             complement(16476..18209)
                     /locus_tag="PKM49_01230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNY81468.1"
                     /translation="MIGYLCKYTPVEILHGFSETFERIEPITQSVCCADGMLHPNLCS
                     YARAVLETCLEKKITGLVLTNCCDSVRRLYDVALNDPRFTWVEFIDLPRKHDASAVAY
                     FEGEVHRFLERARRFFPRSFNEERFFRSIPRLRPSNPEPDPNPPFPPVRLALTGARIH
                     PDLKAWFMSYPVSIVADTTCTNPDRGDIFQGEISSLKTYARALLNQTPCMRMNRAGGL
                     AEPLLRSRPEGIVYQTVKFCDFYGYDYALLRGELEPPLLKIETDYTTGSVGQLKTRFE
                     AFLESILARREPKKPSSIPFGLLKGEPEMRNDLQKLRVVAGIDVGSTSVNVVLMDENH
                     RILATSIVKTGPKSGLGARNALDEALATARLSLSDIRYIVSTGYGRAVASFSHEAVTE
                     ITCHARGALWLDRNVRTVIDIGGQDSKVMRIDSNGELKDFVMNDKCAAGTGKFLEMMA
                     RTLELEIEDLGPASLRAKEKVMISSMCTVFAESEVISLIAQNKETPDIIKGLCDSVAT
                     RTAALVSRVGKEERVMMTGGVAKNVGVVNSLSERLQTDLVLPPEPQIVGAIGAALIAL
                     ERGHPPRPLSE"
     gene            complement(18206..19459)
                     /locus_tag="PKM49_01235"
     CDS             complement(18206..19459)
                     /locus_tag="PKM49_01235"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNY81469.1"
                     /translation="MTIQALYCKMTKARFSEFGKAQKWIRRGASLQHFRWKYFPDRSI
                     PSSLQALNRVVFQYLAEPLCHPARSAYVNLFAPCEILHAFDLYPMFVESLATFLNGFS
                     CEDLAVDQSESSGLSETLCSFHKTFIGGAERGALPKPLFAMASSVLCDANTKTFLHVA
                     KRLGIPALLIDVPYTPSPEAVRYVSDQLQDVVKQVEDISGKPFRIDRLREVLALENET
                     KRLMAECLEALALKAFPNHLTLEMGRLMLTHTGIGRPESSQCFQALVRDIRNAPPRSG
                     KAIFWIQVIPFFDPTLRARFNYASDYQLLGMDINFDDLEPLPLDDPLFSLAKKIVNHV
                     FNGPFNRRIDWILGLMDRLKPDGVVYFCQWGCKQSSGGAAMMADALKTKGIPCLVLDG
                     DAADRRNMPQGQTKTRVEAFLEMLP"
     gene            20267..22519
                     /locus_tag="PKM49_01240"
     CDS             20267..22519
                     /locus_tag="PKM49_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HNY81470.1"
                     /translation="MIFRKDSLVLIIILLVCFCSSDIIFGASNLESILKRGEIRILMV
                     EEDFWPFFYVDERGEMAGIDVEMGKEIARALGVQPVFVRKGIFNELAGYLEEDVADVI
                     ISYYSYTPYRGARLFITQPYLMDSDGILVSHTLKEFMRNQKIAETPDETVKYLNQRRS
                     LRVGTLADTSYEAWAVETFPRARLWKAKAEEVARLLTNRELDVFYCAHYWLQFFLSLH
                     PEILLNYQYVRLERKDLISMAVTQENLPLYFWLDGYISSTSSRNQQLDELIARYGPAE
                     EEELAPTIVERALSDPWRSVLLAAFLAVDVIIVCASQRRMRGKMVSGKPPHWLLNTWT
                     VLFSLLLGTYTGIAFPEVTGLLSPFGDVFFNYLLLAGIPILFCVVILNFARLMSNIGG
                     LRFFLKFMGILVIFVSIGAFLGLAIGLVIQPGAALPLESQQQLARTLQRQSAEPSLAI
                     DTSPGAMLWMIPSTMIPNSILKAFVENRTLSILFFGILIAFILLGQERKPREKIISVL
                     TTVNDLFFTLFKLSYYILPFGLFCLMMSQASAFTDNSGLFIAFFKLLICQGIMLAAWF
                     FIAIAVSLWKVPMPLPRYLSAIKKPMMIFFSLLSTLATLPAIMEVCEREEAFQDENVK
                     GTLPLLLIMLIPTIASIFSLTIVFLVQLLRLDIGWVGYLFIGINSVIAGIAASGIPAP
                     ADLYSVSIVTAPLGIPVTQALFFLLPYTLTGARIDSAFCVILNFAIAQFFKKETKPRP
                     VATQTASSDA"
BASE COUNT         5149 a         5992 c         6056 g         5417 t
ORIGIN      
        1 tccggggcga atccgcccga aaaccttact ctggctggtg atatgtatta cctttctcgt
       61 cggaagcggg ttcggttact cgtttctatt cccgaacctt gtgtactatg agagggggaa
      121 ccttctagag ctatccacct ccttcgaagg cttttctgta ttgatgtccc ctcgttttct
      181 gatgccgacg gtgtggacct atcgggtgca ggacccggtt atcgccgctc tcatcaagac
      241 cgaatcggat ttcattttac acaatcgctc ttcctcaaac gcattgggaa tataccagat
      301 gaagccgttc ttcgcttcca gcctagggat ttggaatcca ttctttcctt ggaatgagca
      361 gaaggtatcc ggctatttag aagaccttca aaagaagtac ggaagcttgc gggctgcatt
      421 gaccatctat catcgtggcg aggccggata cctctctatg aaaaaggatt ccaacgcatc
      481 cgacaaactt tacaactacg ccgaaaaaat cgaagggttt caacgtcaat attcgaaagg
      541 ccagatggtt cgactgaaag acttttggcg tatcggctta ggtttttctt cctcttggaa
      601 gggggggaac tccggtcggc tgaccgtggt cgcccccacc gcgtggttcg ggacctcggc
      661 cgcggtcttg acggtagaag acgccgttag gatcgagctc tgtcaggagt ttatgatttc
      721 ctcccagttt caggttttcg tcggataccg ggaatccgcg gttatcgggt gctcgttccg
      781 gagtcagacg tggaacgacg ttttggccat tcgctatgat ttcggaacaa gagaactgtc
      841 ttgggaagga aagtttgaga agagtcccct gttcttctct ttatccggtt cggttgaagc
      901 ggtaaccgta cggtgcgggt ttatcttcga taaccgttat caggtgacct taggtttgaa
      961 atgtggcgca tcgttggccc cgttcttttc cgcgaaggcc gttttttagg agtttaggag
     1021 cggactagag cgatagaacc ccgggtcctt cgtggaggta gaatggaacg cctaaaagaa
     1081 gtgtggatcg tcaaagaaac ggaaaaagaa ccctggataa tcatagaaaa gtctaaaccg
     1141 gcttcctgtg agtcatgcgg ctctatgtct ttttgccgtg taaaaaacgt ggtggagaca
     1201 agggcgaaaa acgaaaccgg caaaaccctc aagcccggtc aacatgttct cgttgaggtc
     1261 cccctcgttt cgtcgacgaa gacggcgttt ttcctctatg gcatcccgac cctcgccttg
     1321 gtcgtctcgc ttcttctgtt tatcgaaggg ttcggcctgt cgcaaaccca agggctgctc
     1381 tacgccctgt tggtcttagc gggggttttc ctggctttgt ggttctacga caaagcctac
     1441 gcgaaacgtg aaacctatac ggcccggatc ctagccgttc ttcccgagat gaaaagcgtc
     1501 gatcgaccgg aaggccgcga tgccctttga acgcgttcag gggagctatc ctgttcaaga
     1561 taatttgctt ctattagggg gaaatacgtt gaaaaaaccg ttcttatcga cgatggtact
     1621 ctttcttttg ttgttgattc tacctcgagt cggtctggcc atacagatag gctttctgga
     1681 aatcaccggc aacgtccgcg tcaccagaga agagatcgtg gccaacctcg tgaagtgcaa
     1741 ggaaggatac tcggtaaccc tagccgatat agacaacgat atctttcggt tacgggatat
     1801 ggggatattc caggatgtga agtaccagtt gacgaagtat atcggcgaca cgatgacgct
     1861 caccttcgaa gtgctggagt atccatacgt gaaagaggtc aaggtgcggg tagaagggcc
     1921 ggacctagtc gatacgaaag acatgaagca ggcgatcttg gtggaagcca accaggttct
     1981 aaactataaa aaactcttcc gtacgatctc cgcgatcaaa acatttttta tgaactccgg
     2041 ctatcatacc gtcgaagtga cggacaacct tcagaagacc gatggcggcg tttttttacc
     2101 ggatcagacg ttgatactga cgataaaaga atacggactg tacgacgtga ttctcaaagg
     2161 ggagctcggc gacatcacct atagcgaagt cagagaactg ctcggcgtcc ggacgttcaa
     2221 ggattattac gaggacttct ggcgctggct gcggatcaaa tcgaagtact acccgtcgat
     2281 ctccgactat cagatggggc tgtccagact cttcagcacc ggcttgatcg ggccgaagac
     2341 gcagtacgac ttccaggttc tgaaggagcc attgaagaac ggggaacatg tggtcaatct
     2401 gattttaacg atcgagctga acccagtcgt cccccgtgac aaagaggttc agacgattct
     2461 atttaccggc aacactttgg tgagcagcga caagcttcga gagggcttaa ggagcctacg
     2521 gaaccctaaa acggttttga tcgatgtgct ccgggacgcc caaaagataa aaaagaccta
     2581 tgaggaagaa gggtatccgt ttgtgatcgt gacgccttcc taccaagagc aaagcaaggc
     2641 gctcgtcttt acggtgaccg aggccgtgat ctcggatatt cgtctggagg ggctgacgaa
     2701 aacacaggaa tacttggtct ggaaagagat acgtctgaaa aggggcgaac cgatcaagat
     2761 gaggaatctg aagcagacct acatcaacct caaccggacc gggtacttcg aatcggtgga
     2821 tatcgagccc ttgggttttt ccgcgggttc cacacccacg gtcttggtga tccatctcgc
     2881 ggaagcttgg aacaacctcg ccgtgaacgc gggtgtttcc tatgatccgc gaaacgaggg
     2941 gtcgactttc gcgcagaagg tctacggcac gatctcgcta gccttaacca atcccttcgg
     3001 gtatggccaa acgatcgact tttccgccac cctcgggaag tatccggcgc tgaaatttgg
     3061 atacaagatc gccagcatct ttggctctca cttcgacgcg ggagtggatc tgtcgtatca
     3121 ggaccagtac accagccgtc gcgtctctga attgagcacg accgtcacct acgaggaaga
     3181 gaagtatacg atcatgccgg aactcacttt ccatatcacc gattcccaaa gaatcacggg
     3241 ctccttttct tggggcaagt tctcacgaaa ggacttttcg atcgatgaag ccaccgcgac
     3301 caagtatatc gaacccaaag gggacatctg gacactgtcg ttgatgtata cctttgacac
     3361 gcgggacgac ctgggcgatc cgatggaagg tatcaacgcg ttcgtaagag gagatttttc
     3421 cctacccttc gcaacggacc cttggttacg atttcaggaa aatttctcga ttttcttcag
     3481 ccccttcgac taccaccata ccttaggggg gcggcttctc ttttccgaag aggcctggga
     3541 tccgcacggg gtactctcat ataccctagg cggcgcgaac aacttcttcg tgagagggat
     3601 cccctatgac cgcgcgtcga cgaccgatca cttcggggtc ctgaatttag agtaccgctt
     3661 tcgtttatcg aaacagggag agatgggagt ctccttcgcc gccttcctcg atcaggcgtt
     3721 ggctttcaac gacctttcga gcgtttcaat agataactgg ctggtcgctt tagggggagg
     3781 tatacggctc acgatacccg gtttcggaat tttgcgtctg gacatcggat gggatttttc
     3841 gcccaccttg tgggacgaag gcgggccaca atgggggggt ttccatttcg ggttcggcca
     3901 gatgttttaa accggtcata tgcgctctgt ttctgatcgt cgcagctatc tccacgctct
     3961 tcgcccggat tccgtggttc accggaaccg tggagtgcct gtatgtgcaa ggcatctcgg
     4021 gggcgctcaa agagattggt caaatagaga acctgtcgaa aacctatgag atggacctgc
     4081 gagaggcgat ctggaagaga gagatacgga ggttctgcca acagcacaac ctatatttgc
     4141 cggtagcgga aacgtttttc acgaagaatt gggtgttggc ttggtatgat gcacaaggga
     4201 cagccgagcc tttttccgtg gatcggatac aagacctgtc ctggacgctc catatcttga
     4261 gagacgcttt gagaaatgga gtggtggcct tcgaggactt ccccggcgcg agcgaatggc
     4321 tttccgaagt ctacggcggc cctttggcat cggtgaagaa cggagagact cgttttagct
     4381 cggcctatcc taacctattg cagatggcga tggaaaaggt gatctatcga gaggacttct
     4441 cgaagtccat cgtttttttt caagaactgc cggagtgtat cgtcgaagtc aagaacgcgt
     4501 ataagttcct gcaaaccgag acgaaaacgg aaaaggccta ccacgcgcag tacgcccgtt
     4561 tctccgaaaa agaggcatta gccgccatcg acgccgcgat gtttctggaa gagatcgaac
     4621 gcgtaacgct gacgaatgtc ctgagcttta aagagaacat ccggagatgg ttcattcttc
     4681 cgccggaaga caaggccttg gtccgttttt tagatgcgct ctcggacaaa acggcgttgg
     4741 tcctctttca atcgggcgaa gcagattggg ttttgaccct ctttttatcg gtgccctccg
     4801 atacctttcg ggagctcttc gaatcttggg ggatcggggc gacagagcgg cctttcccgc
     4861 gaatcgtgcc tttcgcggac gcgctttgct acttttttat agaggggaat cgattgcatt
     4921 tttcgaatcg ctccgcattg cccaatcgag agttgcccgg ggcggcgaaa gaagagatgc
     4981 ggcgttcggt cctggaatca aaggcatacg agatcgacta ctcgcggacg ccttcgaaag
     5041 gagcgtcgtt gcaccgcctt ttatcccgtc gctatgaaaa caacctgcta ttagagagtg
     5101 aaagggtttt ctgatgagat ccgaacggtt tgacgccgag atcgatatgc cgaaccacgc
     5161 catccaacgc ggctatgagc gagagctgac cgctcgagag atcatcgacg ctatcgatca
     5221 tccccaggcg cggtgtcgtc tgcaacacaa cgggagattt cgtatcgagt cggggccggt
     5281 ggtggtcatc gttcaaaaag accttcacgg gtttcgtgtc gtaacggctt tttttacaaa
     5341 aaggagggac acctatggca aagactcttt tccttagtct gcttctcggt atttgttcgt
     5401 tgtctttcgg gctatctatt ctggatatca ccttgacctt agagtctcga ctgctctttg
     5461 agacccaaaa atccaccgaa gaggtccacc tcctgctacc cgcgctcgtt ccgttaaaag
     5521 ccttcctgat actggatgag gaggcggcgg aaagccttcg tatcggttca agtcgctatt
     5581 tacttctggc ttacgcctct ggagaatggc aagagcactc tctccccgcg ctgtctccgg
     5641 tttctcccgt taacagccat atcaaagcgg cctctaacta tatggccctt ctcacgtctt
     5701 ttttagaagc tgacacgctg gttcagaacg tctcggtcta tcacctcttg tcctcgaaga
     5761 tggagtattt ggacatcttt ctggacgagt tggaacagct ccagctttcg ctctacgagc
     5821 cttttaagaa gcaggcgctt cgggtcgcct ttgccgatta cttatccctt ctacacgaag
     5881 ggaaaaagca attcttccta tcattgccga agtttttcct acgagaaaac gtagaattca
     5941 aagttttaga gtttgagccc gccttttcgg atttgatcga acaaacgatc tctgagtgga
     6001 accggttgat ggatcgggtc cacacgcctt tacaattcga ttccttcctt tccggcttat
     6061 ctgtcttttt ggaagggctc gcgctcaacc caaaaaagcc gatcggggcg acggtcacgg
     6121 aatggatgaa gcgtctggat tcgggtgaag tcagtgacgt caaaatacgc accccctatc
     6181 accatccttt tttttccgcc gagaacaccg ttttcgccga cctgcaaacg gcgctcctgc
     6241 tggataccca gaaggaattg gccatctgga ttcacgagga gtaccgaaaa atggtcctgt
     6301 atgcttcacg cacgctcggc ttcttgacgg acagccacat cgaaagaata ctcgcgggcg
     6361 tgttcctgtt cgaactatgc tctctgaaga caatggcgct gatcggtcgt tacggcaacg
     6421 agaccgcgcg ccgaaaagtc ctggatctct acaacacgca atatacaccg ggacaggatt
     6481 ctcaagaact ctttccgcag acggcggaac tgatccgcga ggagctcgtc cagatgatga
     6541 tcggtatttc cgatctcggc ggcgccgcac ctctcttccg agagtcagcg ggcacgactg
     6601 ttccggccct tgaggttcga cccgtcacca cgccgaaaac ctattcgctg gccagcgcgg
     6661 agttggttca catgctggag ctcgagacgc cactcgggca ttttgccgaa gcggtgatgt
     6721 cagtgcgtgt cggtaccaga aaagccgaca ccttcacgaa aatataccct gtctttccta
     6781 cacagacgac acggggcaag cccctcatac tattggactt cttttggttc gcgattaagc
     6841 agtggggaca gactttggga aagctgctcg ggttaatgct taacagaacg atcgatacca
     6901 tcgaaatgga aaatcgtttg gaattgaagg gagtagaagt gaccattccg cgctactacg
     6961 aaatgcgccc ttatgccgaa ttgtggcgga tgcagccagc ggacgatcag aacattttcg
     7021 tctccgttca aaacaacaac tccgaaaaga acgcggtgag caatctcttt ggtggagtca
     7081 ttcccgactt cttggccgga gacatcgaca agatcccggc ggatcccatc ttcaggatcc
     7141 atcggtatcc gacagaggtc ttcaccacga ccaaaggcct taggaattcc gctccccgtg
     7201 gaagccgagc cttgatcctc gtgcacggga aacaagcgct ccccaacttc acgagagaga
     7261 aagaggtgat ggttcacgtg ccctatatct ggcggacgga accccggctg gcggtgtggg
     7321 atcgacttta ccttctatgg acggagagag ctcgggcggc agaaaccggt tccgttttcg
     7381 aagcctacga tttttacgag ttcgtctacg acacgtcggt gttgaccgct cgggaatacg
     7441 ggcagatatt ggcgggtatt ctccaggact acgggttttt acacgactat gagacgctcg
     7501 tcatcgtcgg gcacagtatg ggtgggatag tggccaggga agcagccaat acccgcctcg
     7561 aacgtccctc tgacccccaa aaagcctttt tgggagatta cttgagcgcg atcatcactc
     7621 tggactctcc ccactacggc tcggtcctgg aaaatttcgt ctacacggtg aacgccgagc
     7681 tgaaagcgcg gatcaaagag tccaaacaac cggacgatgt ttttcgtatc aatctgtacg
     7741 agttgttgag agcccttttg agggacgact acgtggatca aagccttcaa gtcctcctgg
     7801 cgatcacggc gaaaaatccc gaactggtcg gtaaggtgtt caccgagatg gtctccttcc
     7861 tggatccctt tccgggaggc gtttgcctga gctatacggt cgactcctac acacgagccg
     7921 tcgcggacta cttgtacgga aaggtggatg cgcgccgata cagcgacatc ttaaaaggcc
     7981 ccgaagattt gcggctcctg aatcagaatg atcggttcta tgaccggcta tacctcttca
     8041 catccgagat tgacgaaaac tccgaactat cgaattccaa ctatagtttg ctctacgcgc
     8101 taatgaaaac gacgactcaa aaagtgacct tctacacgaa ccgggatttt tctgtccacg
     8161 aggccaacga ctgcgtcgta tcactctaca gccaacagat ggctggaagc gacaagggac
     8221 agacgcgtat agccttccaa gggatagacc ataccgcggt cccgatggat agagccgttc
     8281 tcaatcggtt gtttcaaacg atagaacaag tttcgggacg gtgagcgaag cgatgacggc
     8341 gactcgcgag aaagcgttac aggcgatcga agggttgatc ctatacgccc tcgaacgaga
     8401 tctgatcgct ccggaagaga tccattacac gcgtaacacc ctactcggtt tcttacggtt
     8461 agaagtccct ttgagcgatg tgaagcagcg accggtggaa ccattgccgc ggtatttgga
     8521 cacgttattg gactatgcga gagaacacgc gctcttggaa gaagaaacac aaactcgcag
     8581 ggacctcctc gacaccgcaa tcatgggtct gctgaccccg cgtccaagag aggtggcgcg
     8641 ccgattctat gagcatcggt tgatcgatgt cacgaaagcg acagattggt tctaccggct
     8701 gtgcatagac acgaactaca tccggatgga tcggatgcaa aagaatatca agtggcgatc
     8761 ggaaacggaa tacggggcac tagagatcac gatcaacctc tccaaaccgg agaaggaccc
     8821 tttggaaatc gccgcggaaa agcttaagcc ccaaatcggg tacccgcggt gtttgttgtg
     8881 tgtggaaaac gtcggtttcc acggaaacct tcgacacccc gctagacaaa acttgcgggt
     8941 cattccggtc accctcgctg aagagccttg gttctttcaa tactcaccgt acgtgtacta
     9001 caacgagcac tgtatcgtgc tctctgagga gcaccgccca atgaagctgg aacgcaaaac
     9061 tttcgcccgt ctcttcgatt ttttggatca atttccccat tacttcatcg gatcgaacgc
     9121 ggacctgccg gtggtcggcg gatcgatttt aagccacgac cattttcagg gcggacgggc
     9181 gacgatgccg atggaaaagg cgcctatcga tcgccattac actctgagcc gatttccggg
     9241 tgtggagact gggagggtca ggtggccgat gagcgcgttg cgtttgcgtt ctgacgacag
     9301 aagggtgttg gtcgacgcgt ccaccgccct cttggattac tggcgcactt ataccgacgc
     9361 ttctgttgag gtgatcgcgc agacggacgc gcctcacaac acgatgacgc cgatcgcgcg
     9421 aaagaccgga agcggtcaat acgagatcga cctcgtcttg cgcaacaacc gcagcaatcc
     9481 tcaatacccg ttgggtatct ttcaccccca tcccgagctc catgcgatca aaaaagagaa
     9541 catcgggttg atcgaggtga tgggactagc gattttacct gggagattgc gtacggagat
     9601 gcggcgcgta caagagtgga tgaccggtga tcatcacgtt acccttgcct ccttggatcc
     9661 atcggatgag ctatacaaac atcgagagtg ggtggacccc ttaatttcgg agaaaggcgt
     9721 gggccatagc cccgaacggg cgcgcgctct tatcgaagaa gagatcggcc gaaaattcgc
     9781 gacggttctc gagcattgcg gcgtattcaa aagagattcg aacgggtttg cggccttcga
     9841 tcgtttcgtg tataatttta atcagattcg ggaatcaacg gggggattaa gatgaaaaaa
     9901 atgatgttgg ggatatgcat tctatgtttc tcgattgtgg cctttggggt gattccgctg
     9961 aaggacgttt cgccttccca ctgggcctat gaatctgttc aatacctgat cgaaaaagga
    10021 attctcacgg gcttgccgga cgggactttt cagggggagg cctatttgac tcggtatcaa
    10081 ttctccgtcg caatgtacaa agcctttcaa ttgctggagc gaaaagcctt tacgggagag
    10141 gtgacctcga cccaggacct ttccaccatc aactttcagg tttcaaccct aaaagggttg
    10201 gtcgagacga tcgcggcaaa gatggaacgt atgggccgcg actatcaaga cctagtgaag
    10261 cggattgacc aaatggggac aaataccgac ctggcaaacc aagcctctca aaccgctaag
    10321 ctgctctccg ggcttgaaac gcgtgtgatc gaactggaag tggaaaacga ttccgtgatt
    10381 atgaaattag cggcgcagga acgggggtta gcggattata gacggatcgt ggaaaatacg
    10441 ggtttggaat atcaaaacct gtttgcgcaa caccaaaaac tgaaccagaa ggttaatata
    10501 ctgataggcg tcgcggcggc cgcttcggta ttggccagcg taggattggg cctcagcatc
    10561 tatttgatcg cgacgcgata accgtgccta ctcgcggccg cgccggccgc tacaagtcta
    10621 ccgaggtcgt cccggtaggg tcgagggcgg tcggagaaat gggatggtga gttcacgtga
    10681 aagtgccatc ggtcgaccgc cctataacga gacccttcag agttcgacga tatgaaccaa
    10741 gtctccggtt ttgagcatag ccgcgttgga ttcgtcggta tcgacgtgaa actctaatgc
    10801 atacgtggga ttgacacgga tcagcacgtc catcaggatc gcttttctcc ctttcgtatc
    10861 acaaaaaacg gctaccagat ctttatcctt gacaccatag cgctgcgctt cttccggcaa
    10921 catatggata tgccttttag cgatgatcaa ccctttgtcc agtttgacgc tgcccttcgg
    10981 gccgatcaac tccacccccg gtgtgccaac gacgtcgccg ctgtcgcgta tggggggcgt
    11041 tacgcctagt ttcagagcgt cggtcatact gatttcgatc tgggtatcct ttctgacggg
    11101 gcccaacacg cgcacgttct cgaatgaccc tttcggaccg acgatcgtca ctttttcatc
    11161 gcacgcgtac tgtcccggtt gccgtaagtc cttatacttc gtcagcgtgg cgccggcccc
    11221 gaaaaggatt tccagatggg cgggcgagag gtgcaaatgg cgcgcagaaa caccgacgat
    11281 gatggctcct tctttgatct tcatcgtaga gaaaaacctc cttagtcgtc ctatctttta
    11341 agatatgttt acgtcgtacg cgactcgttc gcttcgttta catccagcgt attgttgaca
    11401 atacgcagca cctttcgaaa ttcgtcggtt aaaaagggat caaccgtgac gaacaaccgt
    11461 tcgacaaaaa gaaccctccg gcgaataacc tcctggatta acgctttacc ttttggggtg
    11521 atgacgacct ttcgcatacg gtgatcctct tccaaaaccg agtactcgat gtaaccggct
    11581 tctttgagtt tggtaatcaa gcccgttatc gtgcttttcg ccacccccag ccggcgactc
    11641 agattggaca tcgtggagtc ccctccccag taaaccctct gtagcacatc gaactgagcg
    11701 ggggtgatct tataattccc ttctttgatc acctttcttc cttctgcttt gatccaggcg
    11761 gccagtctcc gaagagatct ttcgagctcc ttatactttg acggagcctc caccccggaa
    11821 aaaccgtcgt taccctccat tcagctctcg acctcccaat actatggtgt attctcctcg
    11881 cgcttctcct tcaaaagcag ttttgacctc agagacgcgc ccataaaaaa actcttggtg
    11941 aaccttcgtc atctctttgg caatgaaaat ctgtcggtct cccaagattt gctcgatcaa
    12001 ttcgagcgta tcctttatgc gatagggcga ctcgaaaaac acgatcgccc tctcctcttc
    12061 cttgaacgtc gcgagaagtc gcttcaaccg cttcgcgcga ggtaaaaacc cttcgaagat
    12121 gaacgacccc gagaaccccg agcacgcgaa agcgctgaca acggcgctgg gccccgggat
    12181 gacgtctaca gggatacggt gttcgtggca agccctcatc agttttgagc ccgggtccgc
    12241 cacggccggc attccggagt cggtgagcag aacgccgttt tcgccgtttc gcaaccgctc
    12301 gaggactgcg tccaattttc gttcgatctt ctgttcggaa aaggagatga tcgctttcct
    12361 tccgatttgg taatggttga tcaaaatgag ggaacggcgc gtgtcttccg ccaggatgaa
    12421 atctgcttcc cgtaggattc gcacccctcg gaaggaaaag tcctccagat tcccgatcgg
    12481 ggaggccacc aaggtcatcc ttcccgacgc tttttctatt ctcacactcg ctgtccttcc
    12541 ctcgttaggc ggtaaaaaaa tccttcagca acagttccaa tctctgaagt gtttttttgg
    12601 cttcttccgc tttcagctgc gcttcttcga ccgcttccgc ctccgcgtga gccataaatt
    12661 tcggattgtt cagcttggat agcgcgtgtt ggaggtcaac ccgcgtttta tcgatctcct
    12721 tctcgagacg ttggcgttcg ccttcccaat caatggtctc tcccagtata acatagatct
    12781 cccgagagga atcaacgaac gcggtcgcgg acttctcggg ttttcgatcg acgacggcga
    12841 cctcttccac attcgccaaa aaccggatat aggcgagttc ttcttccaaa agcgcggttc
    12901 ccgactctcc taaaaatcgg aacgcgacgg tcagtttacg agaaggcggg acctgaaact
    12961 gcgcgcgaag gttcctaatt ccccggatct cttcgatgag cgcctcatag cgcgaacgtt
    13021 tctccacaaa agcgtactcc ggcctctctt cgggccaggg cgcgatgatc agagacggcg
    13081 cttcttttgg aacaggcaga tgctgccata tttcttcagt gatgaagggc ataaacgggt
    13141 ggaggattcg gacgctctgg tccaacacat aggccaatac ggctcgagta ttctgtttag
    13201 gaccactatc cccatacagg atcggtttcg cggattccag ataccaatcg cagaattggt
    13261 tccagaagaa ttcataaata gacttggccg cgaagttgta ttcgtaacgg ctcaggaaat
    13321 cggtggtctt gcggatcgta tcattgagag acgacaggat ccatccgtcg gctagggtgc
    13381 actgatcggg atggatagcc agagaggcgc cgccgtttga ggggtccaga ttcatcagcg
    13441 cgaatcgaga cgcattccac agtttattgg cgaagtgttg gtaggtttcg acgctcttca
    13501 cgtcatatcg gatatcgctg ccctgggagg ccaggatcgc gagcgagaag cgggtggcgt
    13561 cggcgccgta ggcttggatt acctcgatgg ggtcgatgcc gttccctagt gatttgctca
    13621 tcttccgtcc ctttttgtcg cggaccagct ggtgaatgta gacgtcactg aacgggatgt
    13681 cgcctataaa ctccatgccc attacgatca tccgcgcgac ccagaaaaag atgatgtcga
    13741 acgcggtgct caacaaagag gtggggtaga agctttcgag atcggaggtt tggtctggcc
    13801 agcccaaagt ggagaagggc caaagtccgg aagagaacca ggtgtcgagg acatcatcgt
    13861 cttgaatgag aaaggaggat ccgcattttt cgcaccgttc cggcgcttct tcagtgacgt
    13921 tcggatgacc acagtcctgg cagtaccaga ccgggatgcg atgtccccac caaagctggc
    13981 gagagataca ccagtctcgc acctcgctca tccagttcag gtagaccttt ttccaccgat
    14041 cggggtggaa cttcactttt ccgctttcga ccacctcgat cgcgggcgca gcgagcggct
    14101 tcatcttgac gaaccactgg tcggagagac gcggttctat cgtggcggag cagcggtaac
    14161 agtggccgac ggcgtggcgg taatcttcga tttttactag aaagccatcg gattcgagtc
    14221 gctccacgac acgctttctc gcctcgaggg tggtaagccc ggcaaaaacg ccagtttcgg
    14281 gagacatata tgaccgctcg tcgatcactt ccacgtaagg gaggttatgg cgaagcgcga
    14341 tctgatagtc gttgggatcg tgggcggggg tcacctttac ggcgcccgtt ccgaactgcg
    14401 gatcgacgaa atggtcggcg atgatgggga tctctcggtt gacgatcggt agaatcaagg
    14461 tctttccgat ccagtctcta tagcgctcgt ccgaggggtt caccgcgacg gccgtatcgc
    14521 ccagcatcgt ttcggggcgt gtggtcgcga cggtcaggcc tagtttctct cctttcaacg
    14581 ggtacagaat gtaccagagg tggctctttt cctcttcgtg ctcgacctct tcgtcggaca
    14641 acaccgtacc gcaacgggaa caccaattca cgaggtattt ccctttataa atcaaccctt
    14701 tgtggtagag gtggacgaaa gccctccgaa cggcgtgatt cagaccctcg tcaagggtga
    14761 accggtcccg ttcccaatct actgagcatc cgatccattc gatctgattt tttatgtgct
    14821 tgcgatactc attcgcccag tcccaggtga tgcgaaggaa ctcctcgcgc cccaccgcat
    14881 ctcgcgaggt acccttttct cgcaggttct tctcgatgac cccttgagtg gcgatccccg
    14941 catgatcctc tcccggaagc cacagggtgt ttttcccgag catccggtta aaccggatcg
    15001 cgatatcttg aagggtcata tttagcccgt ggcccatatg caacttccca gtaatattcg
    15061 gcggaggaat gacaatggtg aacggctcgc ccttgccgga aggtttaaaa tacccgtttt
    15121 ctttccaagt agcgtaccac ttactctcga tcccttgcgg gtcataggct tttgataagg
    15181 gttccataac aatccctcca tgatcgattc gcgtagattt gcgcaatgcc gaatgaacgg
    15241 aaacgatgag aatgactctc cgagaatgtt cgctattata gcacgagacg cggccgtttc
    15301 gatattctgc gtcgacctag tcaccgtcct gggaacgcct gggagcgccg aacttcctag
    15361 agcaccctgg gaacgcctgg gaacgccaac ttttaagttg gcatgttttc tgggctcttc
    15421 gtcattttac aaggcgtact taattaacat cttctatcgt aataattctg tagaccctta
    15481 gaccgtttcg ctaatccgct ctaaccgcat aatcggccat cttgtggact cttttttcct
    15541 cttatgttac tttgtatttc ttggatgatc agtgggcttc aagtaatgta tcgaagagcc
    15601 gcaaaaacat gccaatctaa agattggcgt tcccagaagg gcacgatcca caacacttcg
    15661 cactctcaag tgtggtatga taaatcataa gatacagagt catacaaagt ccgcgccggg
    15721 aggattctgg gagggaggtg atcacaagag ggtatgcttg acttttccaa agaaacggaa
    15781 tacgcgctag atcgctatat agaaatgatc atcgaagaga acaaacgcca gaacctgatt
    15841 cggtttaccg accacgaggc tatcctgact aaacacgtac tagacagcgc gctcccgtgg
    15901 atggcccatc gcatagagct cgagggaaag ggcgtcgata tcggttccgg cgcggggttt
    15961 ccaggcatcg tgatcaagct gtgctttcca aacttgtcgc tcactctcta tgagtcggag
    16021 aagaaaaaag cggcgttttt gcgccgggtg attcaacaat tgggtttgga cgggtgtcgg
    16081 gttgtcgcgg atcgcgtcga atccgcgggc aggcctcaat cctacgatgc cgtttttgcc
    16141 cgagccgtgg gtgggttgcc cacgctgttg gaatacgcgg cgcctctact ggccgttggc
    16201 ggaaaagcct acttctacaa aggtcccaag taccccgagg agatagaaaa atccgaacgt
    16261 gcccggcgga tactcgggtg ggaatcaccg gagatcctac cttatacgtt cacctacaag
    16321 ggagaactgt atcgccattg gcttttggtc tatcgaaagg atatggaaac ccccgaacgg
    16381 tttccgagga ggttgggtgc ggccgcaaag gaaccccttt aacacgaggt tgtccagggc
    16441 gtattcgcca gacgcgccac gaactcctat cgcttctatt ccgagagggg tcgaggcgga
    16501 tgcccgcgtt ctagggcgat caaagccgcg ccgatcgcac caacgatctg aggttccggt
    16561 ggaaggacca agtctgtctg cagcctttcg ctcaggctgt taacgacccc tacgtttttc
    16621 gcgacaccgc cggtcatcat cacccgctct tctttaccca cgcggctcac gagtgccgcc
    16681 gttcgggtgg cgacggaatc gcacagcccc ttgatgatat ccggggtctc cttgttttgc
    16741 gcgatcagag agatcacctc ggattctgcg aagacggtgc acatactcga tatcattacc
    16801 ttttccttag cccgaagcga ggcggggccc aagtcctcga tctccaattc gagagtccgg
    16861 gccatcatct ccaggaactt gcccgttccg gccgcgcatt tgtcgttcat cacgaagtct
    16921 ttcaattctc cgtttgagtc gatgcgcatc accttgctgt cttgcccgcc gatgtcgatc
    16981 accgttctca cgtttcggtc caaccacaag gcgccccgcg cgtggcaagt gatttcggtg
    17041 acggcctcgt ggctgaaaga ggcgaccgct ctgccatatc ctgtggacac gatgtaacgg
    17101 atatcggaga gcgacaaacg cgcggttgcg agcgcttcgt ccaaggcgtt ccgcgcgccc
    17161 aagccgctct ttggacccgt tttgacgata gatgtggcga ggattcgatg gttttcatcc
    17221 atcaggacca cgttgacgga ggtggatccg acatcgatac cggcgaccac acggagcttt
    17281 tgaaggtcgt tcctcatctc gggctctcct tttaacaaac cgaagggaat cgaagagggc
    17341 tttttcggtt cccttctcgc taagatagat tccaaaaacg cctcgaagcg tgtcttcaac
    17401 tgaccgaccg agccggtcgt gtaatcggtc tcgatcttga gcaggggtgg ttcgagctcc
    17461 ccacgcaaca aggcgtagtc atacccgtag aagtcgcaga acttgacggt ttgatagacg
    17521 atgccctccg ggcggctccg taaaagcggt tccgccagac ctcccgcgcg gttcatccgc
    17581 atgcaagggg tttggttgag cagggcgcgg gcataggtct tcagcgaact gatctctcct
    17641 tgaaagatat cgccccgatc gggattggtg caggttgtat ccgcaacgat cgaaaccggg
    17701 taactcataa accacgcttt caggtccgga tggatacgcg cgccggtcaa cgccaatctc
    17761 accggcggaa agggagggtt cgggtccggt tcgggattgg aaggacgaag gcggggaata
    17821 gaacgaaaaa aacgttcttc gttgaaagac cttgggaaaa agcgccgcgc cctttcgaga
    17881 aaacgatgta cctccccttc aaaataggcc acggccgaag cgtcgtgttt tctcggaagg
    17941 tcgatgaatt ccacccacgt aaaccgcgga tcgtttaagg ccacatcgta gagtctgcgg
    18001 acgctgtcgc agcagttcgt caacaccagc cccgtgatct tcttttcgag gcacgtttcc
    18061 agcaccgcgc gggcataaga gcaaaggttc ggatgcagca tcccgtccgc gcaacaaacg
    18121 gactgcgtta tgggttcgat gcgctcgaag gtttccgaaa acccgtgcaa aatctcgacg
    18181 ggggtatatt tgcacagata cccgatcacg gcagcatctc caaaaaggct tccaccctcg
    18241 tcttcgtttg tccttgcggc atattcctgc ggtcggccgc gtcgccatcg agaaccaaac
    18301 aggggattcc tttggttttc aaggcatcgg ccatcatcgc agccccgcct gaggattgtt
    18361 tacagcccca ttggcagaag tagacgacgc catcgggctt cagacggtcc atcagcccga
    18421 ggatccagtc gattcggcgg ttgaatggac cgttgaaaac gtggttgacg atctttttgg
    18481 cgagggagaa cagcgggtcg tccagcggga gcggttcgag atcgtcgaaa ttgatgtcca
    18541 tccccaaaag ctgatagtcg ctcgcgtaat taaagcgagc gcgcagtgtc ggatcgaaaa
    18601 acgggatcac ctgtatccag aagatcgcct tgcccgatcg gggaggggca tttcggatat
    18661 cccgaacgag tgcttgaaag cactgcgacg attctggacg cccgataccc gtatgggtta
    18721 gcatcagacg tcccatctcc aaggtcaggt ggttcgggaa ggcctttagg gccaaggcct
    18781 ccaggcactc ggccattagc cgcttcgtct cattttcgag tgcgaggacc tctcgcaacc
    18841 ggtcgatacg gaagggtttc ccggagatgt cctcgacctg cttcaccaca tcttgcagtt
    18901 ggtccgacac atagcggacc gcttccggag agggggtgta gggcacgtcg atgagaagcg
    18961 ccggaatccc caagcgtttc gcgacgtgaa ggaaggtctt ggtgttggcg tcgcacaaaa
    19021 ccgatgaagc catcgcgaaa agcggtttgg gtaacgcccc tctctccgcc ccgccgatga
    19081 aggtcttgtg aaaactgcac aacgtttcgg aaaggccgct actctccgat tggtcgacgg
    19141 cgaggtcctc gcacgagaac ccgttcagga aagtggccaa agattccacg aacatcgggt
    19201 ataggtcgaa ggcgtgtaat atttcgcagg gagcgaacaa gttgacgtaa gcggatcttg
    19261 ccgggtgaca gaggggctcg gcgaggtatt gaaaaacgac gcgattgagc gcctgcaaag
    19321 aggacgggat gctccggtct gggaaatact tccaacggaa atgctgcagg ctcgcccccc
    19381 tacgaatcca tttttgggct ttcccgaatt ccgaaaacct cgccttggtc attttacaat
    19441 agagagcttg tatcgtcaca tcctgggcca ccctttcaca cgataggaat ggcaaatttt
    19501 accactaacg cgtagaaaaa ggaaaaaggc gccttgggaa cgcggaccgt ctgtgaacgc
    19561 ctggaacttc tgtgaacgcc aacttttaag ttggcatggt tttaaaagat ggttctcgca
    19621 cagaaagcac agaggagagg ttcgcgctgg ggtagaccct ggaagcctct tgagaactcc
    19681 tttctgtgaa cgccgctctt atgggtgcgt cgaacttttg tccggcgggg ctttgggttc
    19741 gcacttctgg gaacgccgaa ctttagttcg gcatgccttc tgttttgctg ggggcctctg
    19801 ggaacgccca gggaccacct ttccgggagc gcgctgtgag gacttaatcg catacgccgc
    19861 gatgtccttg agctattatc gcggggtaac cagcgggctg acttcggaag cccacgaagt
    19921 ctcgcgatcc gccctttgtc tttaaaaacc ctgccgaact aaagttcggc gttcccaagc
    19981 gtatcgcgcc gtcgcgagaa gaaggggcgc ttacgccgcg atgtctgttt actccgtgcc
    20041 ctctgtgttc tctgcgtgaa accctctctt ttctcttcaa aaaaacctgc caacttaaaa
    20101 gttggcgtcc ccaaaaaccg taaaacttgc cgaactaaag tccggcgtta ccagagaagt
    20161 gccccagccg ggccgcacta tttcttcctc gcgggttgtg ctataatggt agcaactacg
    20221 agcggcgcac tagaaacggt aatttagaag gggagttaaa aaaacgatga ttttccgaaa
    20281 ggattctttg gttctcatca ttatccttct tgtctgtttc tgttcctccg acattatttt
    20341 cggagcgtct aacttagaaa gtatactcaa acgcggcgag atccggattt tgatggtgga
    20401 agaagatttc tggccctttt tttatgtcga tgaacggggt gaaatggctg ggatcgatgt
    20461 ggagatgggg aaggagatcg cgagagcttt gggtgtgcag ccagttttcg ttcgaaaggg
    20521 gatatttaac gaactcgccg ggtatttgga agaagacgtg gcggacgtga tcatctctta
    20581 ctattcgtac accccgtatc gcggtgcccg cctgttcatt acacaaccct atctgatgga
    20641 ctcggacgga attttggtaa gccacacttt gaaggaattc atgcgaaatc aaaagatcgc
    20701 ggagactccg gacgaaaccg tgaaatacct gaaccagcga cgatcgcttc gagtcggtac
    20761 tttagcggac acttcctatg aagcttgggc ggtggaaacc tttccccgcg cgcgtctctg
    20821 gaaagcgaaa gcggaagagg tagcgcgcct ccttacgaat agagaactcg acgtattcta
    20881 ttgtgcgcac tactggttac aattctttct ctcccttcat ccggagatcc tattgaacta
    20941 tcagtacgta cgactggaac ggaaagacct gatcagtatg gcggtgactc aagaaaacct
    21001 gccgctgtac ttctggttgg acggctatat ctcctctacg agcagccgaa atcagcagtt
    21061 ggacgagctc atcgcacgct atggcccggc agaggaagag gaacttgcgc cgacgatcgt
    21121 cgaacgagcg ttgtccgacc cttggcgctc ggttttgttg gccgctttct tagccgtgga
    21181 cgttatcatt gtgtgtgcga gtcagaggcg gatgcgcggg aaaatggtat cgggaaagcc
    21241 gccgcattgg ttgttgaata cgtggaccgt tttattttcg ctcctactgg ggacgtacac
    21301 cggaatcgct tttccggagg tcacgggact tctgagcccg tttggtgacg ttttcttcaa
    21361 ctacttatta ttggccggaa tccccatcct cttctgcgtg gtcatcctca atttcgcgcg
    21421 tttgatgtcg aatatcggtg gcttgcggtt cttcctgaaa tttatgggta tacttgtgat
    21481 ttttgtttcc atcggcgctt tcctagggct ggcgataggt ttagtgattc aaccaggcgc
    21541 tgccctccca ttggaaagcc agcagcagtt ggcccggacg ctacaacggc aatcggcgga
    21601 gccttctctg gcgatcgaca cgtctccggg agcgatgttg tggatgatac ccagcacgat
    21661 gatccccaac agcattttga aagccttcgt ggaaaacaga accctgtcga tcctcttctt
    21721 cgggatcctc atcgccttca tcttgctggg ccaagagagg aagccgagag aaaagatcat
    21781 ttccgtactg acaaccgtga acgacctgtt cttcaccttg ttcaagctct cctattacat
    21841 acttcctttc ggtcttttct gtttaatgat gagccaagcg agcgccttca ccgacaattc
    21901 gggcttgttc atcgcctttt tcaagctcct gatctgtcag ggcattatgt tggcggcgtg
    21961 gtttttcatc gcaatcgcgg tgagcctttg gaaagtgccg atgcccctgc cccgttacct
    22021 gtctgcgatc aaaaaaccga tgatgatctt cttctcgctc ctgtcgacgc tggcgaccct
    22081 accggcgatt atggaagtct gcgagcggga ggaggctttc caggatgaga atgtcaaagg
    22141 aaccctaccg ttgctgttga tcatgttgat tccgacgatc gcgagcatct tctcgctgac
    22201 gatcgtattc ttggttcagc tgctgcgtct cgatatcggc tgggtcggct accttttcat
    22261 cgggatcaac agcgtgatcg ccggcatcgc ggcctccggt atcccagcgc ctgcagacct
    22321 ttattcggtt tcgatcgtca ccgcgccgtt gggtatcccc gtcactcagg ccctcttctt
    22381 cctcctgccc tacacattaa cgggtgcccg catcgattcg gccttctgcg tcatcctaaa
    22441 cttcgcgatc gcgcaattct tcaagaaaga aacgaaaccg cgcccggtgg cgactcaaac
    22501 ggcctcgagc gacgcttgat acgacttttc ggcgcctggg aacgcttggg aacgtcggcc
    22561 tttagtacgt ccagataagg cggaaatgtt cagcaggaag aaagcaagcc tgcc
//
LOCUS       DAOPXN010000011        22527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2302_length_22527_cov_5.531684, whole genome shotgun sequence.
ACCESSION   DAOPXN010000011 DAOPXN010000000
VERSION     DAOPXN010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 22527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22527
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_22527_cov_5.531684"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1446)
                     /locus_tag="PKM49_01245"
     CDS             complement(142..1446)
                     /locus_tag="PKM49_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNY81471.1"
                     /translation="MNKRLGYLKTATAVITFLMLVIVQSFILPKGNANQLAQALIIDG
                     FILLGIYTFRGFRWGHRTSLNALLSSVFVGSLFGSLLSLIPIYFFPQRVSSLFLLLSL
                     LLSIAGAPVLFFIALRLFIKYCPTRRYLVIGKKEQIEPLLEEVRKASFDKIQIYRYMN
                     PSSIALTQELDNASAFDGILVADQQLADHVEPILNQVHDRRIPVEYLPNLVEDTLQRI
                     PLSLIETFKDYYEIAFSKANDSPAKRLFDVILAIAALVFSSPLLALFSLLILIESGLP
                     VIFKQTRTGKGFAPFVVYKFRSLREISRSKLDKLENPNQTIEQRATKVGKVLRLTRFD
                     EIPQFWNVLKGDMSVIGPRPEMAAYHEMGMQRIPFYRFRYSLKPGITGWAQIHYKHTS
                     SPEEYAVKTEYDLYYIKNRTILLDLQITLHTIETVLGLRGSK"
     gene            complement(1488..2783)
                     /locus_tag="PKM49_01250"
     CDS             complement(1488..2783)
                     /locus_tag="PKM49_01250"
                     /inference="COORDINATES: protein motif:HMM:NF010191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HNY81472.1"
                     /translation="MEKITGIIVEYNPLHKGHGHHIEQAKGLTRCDAVVAAMSGDFVQ
                     RGEPAIVDKRARAEMALGAGVNLVIEIPAVYAIQDAKGFALGSVGVLERMGVITDLVF
                     GSESGNLDELDRLADILHHPDDALLEKQRSYMKSGLSFPNARKMALAALSRGEETTPE
                     ATLALSNNILAVEYLKALRHYNSGIKPATIRREGAQYTDLTQNERFSSASAIRKWIRE
                     GRNDLVEGAMPPQAHRILAREIGHQRGPAFLDAFSSVLIPFLHARNRDHLKRYSGVVE
                     GLDLRFVESAKGANTIPELLDAVKTKRFTYAKLRRILLYMLFEIEDEIIQESNAYGPQ
                     YIRVLGFDSVGQEVLKRLRSRSRLPVLSIGSAYLKMARHLEKPRVTERLDTTFRYALF
                     RKQIELNFRMTDFYRTAFFTQNERPVMDIQTPPVRLLEI"
     gene            complement(2759..3553)
                     /locus_tag="PKM49_01255"
     CDS             complement(2759..3553)
                     /locus_tag="PKM49_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81473.1"
                     /translation="MIVFENVDFAYDPRSPFRKDIFQGLSLSFDQGDFHFIIGKNGTG
                     KSTLLQLCDALLFPQSGTITVEGLKTNDRKALPKIRQRVGLLFQYPEAFFFGPTVRDE
                     LCFPLKNWKTPVEKWEGRILRSLEMTGLPREILDRSPFLLSGGEMRLVAIAAMLVTEP
                     TYVLLDEPTVGLDHAGRKRVREMIRAMLDAGNTVILVAHELQLAFDIAYPEPTFHLLE
                     KERSPKRCRLPQLLEIEDLEERYGIGFPSLVEYERIKKGWKRSPVS"
     gene            complement(3540..4364)
                     /locus_tag="PKM49_01260"
     CDS             complement(3540..4364)
                     /locus_tag="PKM49_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81474.1"
                     /translation="MIQLQEVNVAYRSEGPSRRVHVFGSNLSFTVSKGECLGICGGNG
                     TGKSTLMKLLAGLLPSFQGEITIDGMSLRQAEHSRKIRRKIGYLFQNPDNQIITTTVE
                     DEIVFGLENYGYPVEEIDAKLNEALILADLSHKREEDPTTLSDGEKQKLLFAAILAMD
                     PDILLLDEPTSMLDLLSAKRFLKVLPTLRRREKTVLFVSHRMEELMGCDRIAFLDKEG
                     LSLFPSPADFFCSIGPELVEITPWIAYLIRRFYRDGAARDVPMATPEEVYRRYDRL"
     gene            complement(4361..5152)
                     /locus_tag="PKM49_01265"
     CDS             complement(4361..5152)
                     /locus_tag="PKM49_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HNY81475.1"
                     /translation="MNALPGLWIGTSGFSYDDWKGVVYPEKTPKTQWFDYYVTLFNTL
                     ELNASFYAFPSEKTIASLCRRAPQPFLFTVKAHRSITHEGQIDGLPVFFNALKSFDAK
                     GFLPLALFQFPFSFRPYRDQVAYLRSCVEGCPYPYCVEFRNQEWVRPSSVEALEKLQI
                     PLVSVDEPELKGLMPRRAFSKDILYIRFHGRNAQKWWNHQEAYERYDYDYKPAELEEW
                     IYPLLESQAPVKVVYFNNHFRGQAVRNAKLFAEQLEKRKAQGNHP"
     gene            complement(5153..5710)
                     /locus_tag="PKM49_01270"
     CDS             complement(5153..5710)
                     /locus_tag="PKM49_01270"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNY81476.1"
                     /translation="MLHSERLFLRPYEIADAKNVLVFRNDPEVRRFLVPGIPYPLSVI
                     EEERWIEGQSKEAECKSFAIILKETGEYIGGCGFNRINWQSRVATIGIFLGQPFWNKG
                     LGTEAVRILIEFAFGELNVNKIKLQVFSFNKRAIRSYEKNGFKIEGVLREEVYRNGQY
                     YDEIVMGLLASEWKDRRSAGETRSV"
     gene            complement(5712..6566)
                     /locus_tag="PKM49_01275"
     CDS             complement(5712..6566)
                     /locus_tag="PKM49_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81477.1"
                     /translation="MKLFFGLTFILVGVLLLFGFFTANLWASVLDNFVAVWPVVFIFI
                     GLSVLARFRTLRWLNVLNGLLIVLFVLFLFFWRWDFGWTGPLQEYTLPTISLREQDKE
                     VKIIFRASSLDVEFVAQSAEEPLIQGTYVSHAQNLVLKQNENVITIDFPRMRAVTLTK
                     NYKKKIKMALPRQYSYKIVIESGTARFFSSFEDNPFRLIEVNAAIGSIESRFMKTAFP
                     IDYVLKSAIGNVDITLPKDMPYHADVRAVFRSIDMQGLQTKKEEVPTTVRIESAIVHG
                     KIAVMGGE"
     gene            complement(6563..7030)
                     /locus_tag="PKM49_01280"
     CDS             complement(6563..7030)
                     /locus_tag="PKM49_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNY81478.1"
                     /translation="MEKRIYRSKTNRIIGGVCGGVGDYLGIDPVLVRLLWVGSILLAG
                     VGLLFYLIAWIVIPEERESTTNSSASTSSTDDSGKAHFYTTGSATKESETTCKAIDSR
                     KGSLIIAVLFLFIGGFLLLNRFFAIFSWKVFIGILFVFLGGVFLYGFFREVKK"
     gene            complement(7062..8087)
                     /locus_tag="PKM49_01285"
     CDS             complement(7062..8087)
                     /locus_tag="PKM49_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81479.1"
                     /translation="MKEENVVDINPLYLIPEDLSLDPESLLAKTRRQFQRWVQQLADR
                     LPVEERLDFKEFVDSQVNWDGFYIPPAQYYEPQWQRLSFIAFLGALFGGALSLYLFGR
                     FFKEDMMAYIIGAPFGSLALVWLFAWLIRHPKKATLVQALLGLGSAGLTLGVVIATVK
                     RTLFPKSKFLLSGLLASLIGFFLLLVMQLFKPKKTVDTRLRREVMESQLAFLLRIVTS
                     AYRTFETSPPALLSRPAAPTAGSVDNPYLSFSLDQLRRATESRDPEMALAAANSLVNS
                     LTAGGIKPHDFEDGGPFTSELLDYYEPFGLVKPGDRVEVLKTAWIDQHGRPVFKGKLK
                     RVAERIF"
     gene            complement(8029..8484)
                     /locus_tag="PKM49_01290"
     CDS             complement(8029..8484)
                     /locus_tag="PKM49_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81480.1"
                     /translation="MVYLLVIAPVYVFSLFWDLLLSQHWLLSPFALALGGSFSLKTPY
                     WIVMLSATAFVWIVPLAYGIDLWLSPIFLLLFFLYYWSIRYFERKNLVFFTAFSVFYI
                     WQKSVSFLRWEISDIIPFLLCLGVMGIRMADERRKRRRYKSSLSYSGRP"
     gene            complement(8491..9462)
                     /locus_tag="PKM49_01295"
     CDS             complement(8491..9462)
                     /locus_tag="PKM49_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81481.1"
                     /translation="MVYVFLGDDRYTKKAEARRIAQQQEAAFVFVELHKKDQWEPFRQ
                     RITTPSLFGNPFLFYLSEFESASVHVQTNAFRYIGVEAQKKAVSVIIDYGGSKANPLF
                     KPFQSFQYDLPKPWKRAEWIEKTVLLAKREGLTLSPGQAALLFERCGPELERIANEIT
                     KVRAASVNGQIETDLFNALIFPYEPENLDDFVFSVLEQDHAAVFRSLYPVFSEYPENL
                     ILFQLIKAAMCLSHLITHHRKQVSRFEDLREISKDTGLNIPTLSRFYGYSFDRAKKRR
                     NLLYAYEAEGVSRILDELWQIDYENKSEGQSVFKNMIGFLDRLSRRA"
     gene            complement(9502..10530)
                     /locus_tag="PKM49_01300"
     CDS             complement(9502..10530)
                     /locus_tag="PKM49_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY81482.1"
                     /translation="MRNPFDKNRPPKLLFEAYLLFYVSLILLFGYLSPLAFSVVLTAF
                     AIIIVLEPVAGWLIHKRRMRFVLAHALTLIVFFSCVLLLFAFLVPSTLKEAGGFFRFV
                     EDFFGKRRWETFFQLRPETKELLSPTIDKIYPKLLSFFESFVSGLQKSSMTLLNFIFY
                     VVLVTAYWTFFFPTFKKSILHLFPKTTTEEARRFLRELYHQLKRYLSSLILVATFVGI
                     VIGIFLLIIGSQYALILGFWGFFTNFIPIVGVALELIPLLILCFSLGIGKSVALLVLI
                     SIVHPVAFIMFLNLMKGVVQLNPVVIILLILVCSQLFGIVGSLIAVPVGIMFRVYWRS
                     FIVPRLSA"
     gene            complement(10530..11546)
                     /locus_tag="PKM49_01305"
     CDS             complement(10530..11546)
                     /locus_tag="PKM49_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81483.1"
                     /translation="MVCVCLFAWSGYAYTIRQVLEGVESSCLAEMGLPRFIWVQRVDA
                     DLGAGYDLANRQVVIGASADAQVFDALHFNSSFQVFTDFSSSTAYLFNMGLTYRFGDL
                     QSTSRKAYAEARKETMLRKIQACELYFEYLKRRKALENADDSDAVAVVKNRLEQIELA
                     YLLIQLQTLSGLSEGEPEAIDTQGLVFSPLRDEDVRSALLNYSMVMQRDLQKSVSLYL
                     KLQTVHERDFGYILGGPGVHYQPEFEPLSSLEEKMREVSMRKMILEHEVLSEILPDLR
                     RQITETNTQRSKLSKSYLLGQTEAAVLKDINQTLAKLEALDYTVCMDILKIEALFRVL
                     QGEW"
     gene            complement(11613..12614)
                     /locus_tag="PKM49_01310"
     CDS             complement(11613..12614)
                     /locus_tag="PKM49_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HNY81484.1"
                     /translation="MKNIVIVTTGGTIAMKKLPAGVDITPEGNQEIMELEGLTNLAQV
                     KMVEFANLPSPHMSPQLMWQLSRRIQGLVDRDEVDGIVVTHGTDTLEETAYFLDITVL
                     TKKPIVLTAAMRNINELGTDGPRNIFSSIRVACSTQAIGAGAVVCLNDEIHAAREVNK
                     TYTSNVATFESPGFGPLGIVDEDGVFFFRKSTVRYHIPTTKIEPHVALLKTYTGDLGE
                     FIDYAVDKGYKGIVLEGFGRGNVPDTIINSLKTAIQHQVAVVLVSRAYKGRVLDIYSY
                     EGGGAYLKKMGVILAHEQTGQKARIKLMVVLGKTSHIEEIRGYFEMANYFKMSESDS"
     gene            complement(12611..13201)
                     /locus_tag="PKM49_01315"
     CDS             complement(12611..13201)
                     /locus_tag="PKM49_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HNY81485.1"
                     /translation="MKVDEARLMLMLITGERGAGKSTFARHLSQRLTLSEGARFKGYE
                     TVFIDKPEGARHLICQPVGFDEKPFRVAVQNLLPYRRILPDTEAFGRMAALLESIDPQ
                     TEGVYIDEIGFLETLDDRLQQSILSALTRCPFAIAVIRLGDYPFLRKVKGLRPDAVLE
                     LTRVNRERLYAQTLSSLGVSQTDPVFRDPEKGGLKK"
     gene            complement(13198..15828)
                     /gene="alaS"
                     /locus_tag="PKM49_01320"
     CDS             complement(13198..15828)
                     /gene="alaS"
                     /locus_tag="PKM49_01320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNY81486.1"
                     /translation="MEKLSGHTIRELFCSFYEGKKHIRLPSASLIPEDPQLLFTVAGM
                     VPFKPIFWGKVEPTYTRVTTCQKCVRTNDIEQVGRTPRHHTFFEMLGNFSFGDYFKRE
                     AIEWAWEFVIDVLKLPEEKIYVSVYREDQEAYDLWLHHIGLPPRKITRLGKEDNFWGP
                     AGPTGPCGPDSEIFYDMGPKSGCPDPRGCAPGCDCGRFLEFYNLVFTEFNQDEKGNLT
                     PLSRKNIDTGLGLERLAAIMQGVSSNFESDLFTPIVRQSETVLGLRYGSSEASNVALR
                     VVADHIRSAVFMITDGILPSNDGRGYVLKRLIRRAMRFGWLYGQRNPFLYRLVEPVVS
                     VMGEPYPEIVNKVPMVTKIIRLEEEKFLKTLEQGMAMLDTLTAHSKRIAADDAFRLYD
                     TLGFPIEVTGEVAQERGLVIDMDGYRDLMEKQRESARAARGDKEFLKMQEIYESIGSE
                     YPVTPFIGYDRNEGHSNVLYLVREGQTVPELHAGEEGEVVVESTPFFSEKGGQASDIG
                     SFSGPSGAGDVTYVFNPFNQLVSHRIIVKTGVLRTGERVLLSVDLPRRRAVARNHTAT
                     HLLHAALRAVLGDHVQQAGSLVEPDRLRFDFSHYEKIRANQVEAIEDWVNERVLMAIP
                     VSIESRSIQEAKAMGAMALFDEKYKETVRVVRVGTHSLELCGGTHVQNTGEIGLFKIL
                     GETAISAGMRRIEAITGLTSLAYVRKLNATLLEACQVSGSQSESLAQTIKKMQEMQKE
                     AEKTIRNLQEKWSLKEIGRPERAVHQLGDASYTVIRLNGLQKESVRSVTDLALEQHQT
                     GMAIVFNEREGMVDICVKVATPFVQNPFHAGNIARRLATELGGGGGGKPDFAFAGGKR
                     PEKLPDVLARLDTYVRESIG"
     gene            complement(15844..16710)
                     /locus_tag="PKM49_01325"
     CDS             complement(15844..16710)
                     /locus_tag="PKM49_01325"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNY81487.1"
                     /translation="MNNPPKDLKKDRFSIKARWTSAIIGGLAISWMFFNIHTFLALAV
                     LSTAIGGYEFVRLILAKTPNAVERLWLSVLLPTLTCLSGLFILLLPEQGMGAIVSLYA
                     LQIPLICVVELLIHREVATIKDRIVDAVFGVFYLPFVHTLGFYIYWKYGWPYALLAVT
                     STWIFDSMAFFVGIRYGKHPLMPTISPKKTVEGSIGGLVGGFFGLWLVIRLLELFWKQ
                     PIFTLWQIGLLSIGLWMSATFGDLFESALKRSYALKDIGKILPGHGGWLDRIDSMVFF
                     IPTFALLLVFFK"
     gene            complement(16694..17419)
                     /gene="uppS"
                     /locus_tag="PKM49_01330"
     CDS             complement(16694..17419)
                     /gene="uppS"
                     /locus_tag="PKM49_01330"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNY81488.1"
                     /translation="MGNPTHIAFIMDGNGRWAKRRGLERSEGHAKGAQVAEATVRLCA
                     DHGIRYVTLFAFSTENWKRPESEVHFLFQLLSEYLLEHTEEMINKGVRIRFMGRMPGL
                     SPALRELIRKIEARSLHCDRLQMILAVNYGGRQEICDGFRKLHEELNTRPPDQKEEIL
                     DTLSEGFYRYLYLPDVPAPDLIVRTSGEKRISNCLLWQSAYSELYFTDTLWPDFDERC
                     FDAALKEYSGRKRRFGGLDEQPS"
     gene            complement(17419..17970)
                     /gene="frr"
                     /locus_tag="PKM49_01335"
     CDS             complement(17419..17970)
                     /gene="frr"
                     /locus_tag="PKM49_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNY81489.1"
                     /translation="MNKYATEAKEKMQKTVQVVDEELKHIRTGRPAPAMLDDISVDYY
                     GAPTPVTQLASIVIDERALIIKPWDKATLKLIEKAILASSLGLTPMSDGNNIRLNFPP
                     PSGEQKKNLVKVVKEKIENGKISVRNIRRDAIKVIKEEQKKGDIPEDDAKAYEAEVQK
                     VTDEYVEKMDDLFKRKEEEIMEV"
     gene            18635..19879
                     /locus_tag="PKM49_01340"
     CDS             18635..19879
                     /locus_tag="PKM49_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81490.1"
                     /translation="MLISFLKHQARYKIHPEIDPLIESISAVHQDTGLYLHIPFCKHF
                     CTFCPYHKFRYDENMLRTYTEAVFREFGLYPPREYDSLYVGGGTPTVHQGLLLELIRF
                     FRPIIRREIGVELHPLDATGELLELMETEGVQYLSLGIQSMHDEVLRHFGRNHSARDN
                     HRALSQVMNAGYKLVDVDLVFDLAAFSSEVVLKDFETVCAYLPHQISIYPMMRFSFAP
                     YRTKNQPAREFETFLQIDKLALRMGYHRDMLWTYRRSGVAGRYSSVTRPFFRGIGISA
                     SSFNGEGFYVNTFDYDRYLTCIANARLPVSKRYRLSDWENALFYLFWSLYRNELNLAL
                     WCDYFPQQKARGRLLFRYLRLLGYLEPISPELWGLTQHGRKAFHRLEEWLTYAMIEPI
                     WNDYATTPRARDSDQPQPARGP"
     gene            complement(19921..20340)
                     /locus_tag="PKM49_01345"
     CDS             complement(19921..20340)
                     /locus_tag="PKM49_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNY81491.1"
                     /translation="MNHAVVYLTKTGHSKKIASAIASALSLSALDIKTRPAIGEVDLL
                     FIVGGLYGKQSDPMMLEFVKGLSPKQIKKAVLVTSSANGSLKQAAVRTALESRGIQVA
                     PEEYCCKGSFLFFFLGHPNSEEIEGAVSFSKSFLTRD"
     gene            20599..21720
                     /locus_tag="PKM49_01350"
     CDS             20599..21720
                     /locus_tag="PKM49_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HNY81492.1"
                     /translation="MKIAVAGGVTGGHIYPAVAVLEYFMKQYPAVEVLYFATPHGLEN
                     REIVRLFPKARIVKLEIRGVLRPFFTPKNLSILYKAARSVYVCKKELASFRPDFSFIT
                     GGYVAVPVGWASHKLRIPTFLHEQNAIAGLANKLISRGSERIFISFPESARDFKLKSA
                     HRILHTGNPVRIAENVDRGVFHRYGINPNKKIVLVAGGSKGSDFVNGLMLQLYEKWRG
                     NNSQVEFLHSCGEAAFLQKISAFPFVKAAPFIQDMHRYIACADAMVSRGGATTVAEIT
                     YYGVPSLIIPWSGSAENHQLINAISLEKHGAAFYLEEREATVALLEKKLTLLLEKKLS
                     QNMRFALKRLRPKMNPAKKIFHEILSALPESLRSEADPR"
     gene            21816..22178
                     /locus_tag="PKM49_01355"
     CDS             21816..22178
                     /locus_tag="PKM49_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNY81493.1"
                     /translation="MEELGFCGIDCTVCPAYQATIRNDAAMREKTAAEWTKMFGNDFE
                     MEDIVCYGCQEREKPVFGYCDKCRVRLCALERGVLTCAECSDYVCEKLAEFYEQVPQE
                     KARMDKRHEVFLRSQRPE"
BASE COUNT         5269 a         5947 c         5978 g         5333 t
ORIGIN      
        1 gttgtttttt cttatttttt ccctttttct ccaaaaccgg tcgaaagtcg ggccaagcat
       61 tctcggaaaa accgtgaaac acacgtgtcg gactgaagtc cggcgctccc aggtgttccc
      121 ggttcgaaaa atactcggac gttattttga cccgcggagc cccaagacgg tttcgattgt
      181 gtgtaaggtg atctgaaggt cgagtagaat tgtgcgattt tttatatagt ataaatcgta
      241 ctcggtttta accgcgtact cttctggaga ggaggtgtgc ttatagtgga tttgcgccca
      301 gcccgtgatc ccaggcttca ggctgtagcg aaaacggtag aacggtatcc gctgcattcc
      361 catttcgtga taggcggcca tctccggtcg gggcccaatt acagacatat cacctttcag
      421 gacattccaa aactgtggga tttcgtcgaa acgcgtcaaa cgtaggacct tgccgacttt
      481 ggtcgcccgc tgttcgatcg tctgatttgg gttttccagt ttgtcgagtt tgcttctgct
      541 gatctctctc aatgaccgaa atttgtagac gacgaaagga gcaaatcctt tcccggtcct
      601 tgtctgtttg aagatgacgg gtaatccgct ttcgatgagg atgagaaggc taaacagcgc
      661 cagaagggga gaggagaaaa ccaacgccgc gatggcgaga atgacgtcga aaaggcgttt
      721 agccggagag tcgttcgctt tcgaaaaggc gatctcgtag taatccttga aggtttcgat
      781 caaagagagc ggaatccttt gtaacgtgtc ctccactaag ttcgggaggt attcgaccgg
      841 tatccgccgg tcgtggactt gattgaggat gggttcgaca tgatcggcca gttgctgatc
      901 ggcgaccaaa atcccatcga aggcgcttgc gttgtccaac tcttgtgtca aggcgataga
      961 agacgggttc atataacggt atatctggat tttgtcgaaa gaggcttttc taacctcttc
     1021 caagaggggt tcgatctgtt ctttcttgcc tatgaccaaa tatcggcgtg tggggcagta
     1081 tttgataaac aaacgcaaag cgatgaaaaa gaggacggga gcgccggcga tgctcaataa
     1141 aaggctgagt aggagaaaga gggaggagac gcgctgcggg aaaaagtaga tcggaatgag
     1201 cgatagaagg cttccaaaca ggcttccaac gaaaacggag ctgagcaagg cgtttagcga
     1261 ggtccggtgg ccccatcgaa acccccggaa ggtgtagatg ccgagtaaga tgaatccgtc
     1321 tatgatgagc gcctgcgcta actggttggc gtttcctttg ggtaggataa acgattgaac
     1381 gatcaccagc attaaaaagg tgatcacggc tgtcgcggtc tttaggtacc ctaacctttt
     1441 attcacatct gtcgctcctg caggcatttt gtcgattata tcacatttta tatttccaac
     1501 aagcgtacgg gtggagtttg tatatccata acgggtcgtt cattttgtgt aaaaaaggcg
     1561 gttctgtaaa aatctgtcat acgaaagttc agctcgatct gtttccgaaa tagcgcatac
     1621 cgaaaggtgg tgtcgagacg ttccgtgacc cggggttttt ctagatgacg agccatcttt
     1681 aggtatgcgg agccgatgga caggaccggt aaacgtgaac gggagcgcag tcgtttgagc
     1741 acctcttggc ccacggaatc gaaccccaac acccggatgt actgcggtcc gtacgcgttg
     1801 ctttcttgga tgatctcgtc ctcgatctca aacagcatat agagcaggat acgacgcaat
     1861 ttcgcgtagg tgaaccgttt cgttttgacg gcgtccaata attctggtat cgtgttcgct
     1921 cctttcgcgg attcgacgaa ccggaggtcc aacccttcca ccactccgga gtagcgtttg
     1981 agatggtcgc gatttcgcgc gtgtaggaac ggtatcaaaa cagagctgaa agcgtccaaa
     2041 aacgccggcc ctcgttgatg gccgatttct cgcgcgagta tccgatgggc ttgcgggggc
     2101 atcgccccct caaccaagtc gttccgaccc tctcttatcc atttgcggat agcgctggcg
     2161 ctcgaaaacc gttcgttctg cgtcaggtcg gtgtactgcg cgccttctcg acgaatcgta
     2221 gccggtttga taccggaatt atagtgtcgg agggctttga ggtattcgac cgcgaggatg
     2281 ttgttcgaaa gcgccaaggt tgcttccggg gtcgtctcct ctcctcggga tagagcggct
     2341 agagccattt ttctggcatt gggaaaggaa agccccgatt tcatatagct ccgttgtttc
     2401 tctaaaagcg catcgtccgg gtggtgcagg atgtctgcca ggcgatccaa ttcgtccaaa
     2461 ttgccgctct cgctgccaaa aacgagatcg gtgatcacgc ccattctttc taatacgcca
     2521 acggacccca gggcgaatcc ttttgcgtcc tgaatggcat agacggcagg gatttcgata
     2581 acgaggttaa cgccagcccc cagcgccatc tcggcgcgcg cccgcttgtc tacgatcgcg
     2641 ggctcccccc gctgcacgaa atcgccgctc atcgccgcca cgacagcgtc gcatcgggtg
     2701 agtcccttgg cttgctcgat gtggtgcccg tgacctttgt ggagcgggtt gtactcgact
     2761 atgataccgg tgatcttttc catcccttct ttatcctttc atactcgacc aacgaaggaa
     2821 accctatgcc gtaccgttcc tcgaggtcct cgatctccag cagttggggc aaccggcacc
     2881 tcttgggaga acgctctttt tccagcaggt ggaaggtagg ctccggataa gcgatatcga
     2941 aggcgagttg cagttcgtgg gcgaccaaga tgacggtgtt gcccgcgtcg agcatagctc
     3001 gaatcatttc tcgcacccgt tttctgcccg cgtggtccaa ccccacggtc ggttcgtcca
     3061 gtagaacgta cgtcggttcg gtcacgagca tcgccgcgat cgccaccaaa cgcatctcgc
     3121 ctccggagag aagaaatgga gaacggtcga gtatctcacg gggtaacccg gtcatctcca
     3181 acgaacggag tatccttccc tcccattttt cgactggcgt cttccaattc tttaatggaa
     3241 aacagagttc gtcccgaacg gtcggcccaa aaaagaaggc ttcggggtat tgaaagagta
     3301 gacccactcg ctgccgaatc ttcggcaacg ctttccggtc attcgttttc aacccttcta
     3361 ctgtgatggt tccggactgt gggaagagga gggcgtcgca caattgcaag agggtggact
     3421 ttccggttcc gttttttccg atgatgaagt ggaagtctcc ttgatcgaat gacagcgaca
     3481 gcccttgaaa gatatctttt cggaacgggc tccgggggtc ataggcgaag tcaacgtttt
     3541 caaagacgat cataccggcg atacacctct tccggagtgg ccatcgggac gtctcgagcc
     3601 gccccatccc gataaaaccg acggatcagg tacgcgatcc acggggtgat ctccaccagt
     3661 tctgggccga tggaacagaa aaaatccgcc ggtgagggga agagcgacag cccctctttg
     3721 tctaagaagg cgattcgatc gcaccccatt agctcttcca tacgatgcga gacgaacagg
     3781 acggtttttt cccttcgccg tagcgtgggc aggaccttca aaaaccgttt ggcgctgagt
     3841 aggtccagca tagaggtggg ttcgtccaga aggagaatgt ctgggtccat tgccaggatg
     3901 gcggcgaaca acagcttctg tttttctcca tccgaaaggg tcgtcgggtc ctcttcgcgt
     3961 ttatgggaga ggtcggcgag gatcagcgct tcgttcagct ttgcgtcgat ctcctcgacg
     4021 gggtatccgt agttttctag tccgaaaacg atttcgtctt caacggtcgt ggtgataatc
     4081 tggttgtcgg ggttttggaa taggtagccg atcttccggc ggatcttgcg ggagtgctca
     4141 gcttggcgca atgacatccc gtcgatcgtg atctctccct gaaaggaggg caacaagccc
     4201 gccaaaagct tcatcagcgt tgatttcccg gtgccgttcc cgccacagat acccaagcat
     4261 tctcccttcg aaactgtgaa ggagaggttc gacccaaaga cgtgcaccct tcgagacggg
     4321 ccctcagagc gataggccac gttcacttct tgtaactgga tcacgggtgg ttcccttgcg
     4381 cctttctttt ttcgagttgt tcggcgaaca gcttggcatt ccggaccgct tggcctcgga
     4441 aatggttgtt gaagtatacg actttgacgg gcgcctgaga ctccaagagc gggtagatcc
     4501 actcctcgag ttcggccggc ttgtagtcat aatcgtaccg ctcgtaagcc tcttggtgat
     4561 tccaccactt ctgcgcgttt cgcccgtgaa agcggatgta gaggatgtct ttagaaaagg
     4621 ctcttcgcgg cataagccct ttcagttccg gctcatccac cgacacgagc gggatttgaa
     4681 gtttctcaag ggcttcaacg ctgcttggcc gcacccattc ctgattccga aactccacgc
     4741 aatagggata cggacagccc tccacgcagc tcctcagata ggcgacttgg tctcgatagg
     4801 gtctgaagga gaagggaaat tgaaagaggg ctaacggtaa gaatcctttg gcgtcgaagg
     4861 acttcaacgc gttgaaaaac acgggaagcc cgtcgatttg cccttcgtgc gttatcgacc
     4921 ggtgggcttt caccgtaaag agaaacggct gcggggctct acggcatagc gaggcgatgg
     4981 ttttttcgct cggaaatgcg tagaaactgg cgttgagttc caacgtgtta aagagcgtga
     5041 cgtagtagtc gaaccattgg gttttcgggg ttttttcagg ataaacgacg cctttccaat
     5101 catcgtagga aaagccgctc gtgcctatcc ataacccagg gagcgcgttc atctacacag
     5161 accgcgtctc gccggccgaa cgccggtctt tccattcaga agccagtagt cccatcacga
     5221 tctcatcgta gtattgtccg ttgcgataga cctcctcgcg caagacaccc tcgatcttga
     5281 acccgttttt ttcgtaggag cggatcgctc gcttgttgaa agaaaaaacc tggagtttga
     5341 ttttattcac gttcaattcc ccgaaagcga actcgatcaa tatacgcacg gcttccgttc
     5401 ccaacccttt gttccaaaac ggttgtccca aaaagatgcc gatcgtggcc actctcgact
     5461 gccaattgat ccggttgaat ccgcagccac cgatgtactc gccggtctcc ttgagtataa
     5521 tagcgaagct tttacattcg gcttccttcg actggccttc gatccatcgt tcctcctcga
     5581 tgacggaaag agggtaaggg ataccgggga cgagaaacct cctgacctcg gggtcgttcc
     5641 gaaagaccaa aacgtttttg gcgtccgcta tctcgtaagg tctcaaaaac aggcgttcac
     5701 tatgaagcat actattctcc tcccattacg gcgatcttcc cgtgaacgat agccgattct
     5761 atacgaacgg ttgtagggac ttcttctttt tttgtctgaa gcccttgcat atcgatggag
     5821 cgaaagacgg ctcgaacgtc agcgtgatag ggcatatctt tgggaagggt aatgtccaca
     5881 tttccgatgg ccgatttcag cacgtaatcg atcggaaagg ccgttttcat aaaccgggac
     5941 tcgatggagc cgatggcggc gttgacctcg atcaaacgga agggattgtc ctcaaaacta
     6001 gaaaagaacc gggcggttcc gctctctatc acgatcttat aagagtattg gcggggaagc
     6061 gccatcttaa tcttcttttt atagttcttg gtgagcgtca ccgcgcgcat tctaggaaag
     6121 tcaatcgtga taacgttttc gttctgcttc agtacaaggt tttgagcgtg agagacgtaa
     6181 gtcccttgga tcaagggctc ttcggccgat tgcgcgacga attcgacatc cagggaggag
     6241 gcgcgaaaga tgatcttcac ttctttgtct tgttctcgca gggatatcgt cggcagggtg
     6301 tactcctgca gtgggcccgt ccacccgaaa tcccaacgcc aaaagaataa aaataagacg
     6361 aataggacga ttaaaaggcc gttgaggacg ttcaaccagc gtaaggtacg gaagcgcgca
     6421 aggacgctta gcccgatgaa gatgaagaca acgggccata ccgcgacaaa attgtccaac
     6481 acgctcgccc ataggttggc cgtgaagaag ccgaagagga gcaaaacccc aaccagaatg
     6541 aaggtgagcc cgaaaaagag cttcatttct tgacctccct aaagaagccg tagagaaata
     6601 ctccacccaa aaaaacgaag agaatgccga tgaacacttt ccaagagaag atcgcgaaaa
     6661 agcgattgag caataaaaac ccgccgataa acagaaacaa cacggcgata atgagggatc
     6721 ctttacggct atcaatcgct ttacaggtgg tttctgactc tttggtcgcc gacccggtcg
     6781 tgtagaagtg ggcttttccg ctgtcatccg tagacgacgt cgatgcggat gagttcgttg
     6841 tggattctct ttcctccgga atgacaatcc acgcgatcaa atagaacaga agccccacgc
     6901 cggccaataa tatcgaacct acccatagca agcggaccag caccgggtcg atccccaagt
     6961 aatcccccac cccgccgcac acacctccga tgattcgatt cgttttagat ctgtaaatac
     7021 gtttttccat acgcatcacc ttcgagaaat tatgatctga attaaaatat gcgttccgct
     7081 actcttttaa gtttcccttt gaagacggga cgtccgtgtt gatctatcca tgcagttttt
     7141 aggacttcca cccggtcgcc gggtttcacg aggccgaagg gttcatagta atccaacaat
     7201 tcgctggtga agggccctcc gtcttcgaaa tcgtgaggtt taatccctcc ggcggttaac
     7261 gagttgacca aactgttggc ggcggccaac gccatttctg gatcacgcga ttccgtcgct
     7321 ctcctgagct gatccaaaga gaaagaaagg taggggttgt cgacagagcc cgcggttggg
     7381 gcggctgggc gagagaggag ggccggcggt gaggtttcaa aagtgcgata ggcggaggtc
     7441 acgatacgca agaggaaggc gagctgactc tccatcacct ctcgacgcag gcgtgtatcc
     7501 accgtttttt tcggcttaaa cagctgcatc acgagaagca agaaaaaacc gatgagagag
     7561 gcgagtaagc cggacaacaa gaacttactc ttcggaaaca aggtgcgctt gactgtcgcg
     7621 atgacgacac ccagagtcaa cccagcggac cccaatccca aaagcgcctg aacgagtgtc
     7681 gctttcttgg gatggcggat caaccatgcg aagagccaaa cgagcgccag agacccgaac
     7741 ggggcgccga tgatatatgc catcatatct tctttgaaaa agcgtccaaa aaggtacaag
     7801 gagagcgcgc ctccaaagag ggcccctaaa aaggcgatga agctcagccg ttgccattgg
     7861 ggttcgtagt actgggcggg tgggatatag aagccatccc aattcacctg actgtccacg
     7921 aattctttga agtcaagccg ctcctccacc ggcaacctat cggcgagttg ctggacccat
     7981 cgttgaaatt gccgccgggt tttggccaag aggctttcag gatccagact aaggtcttcc
     8041 ggaataagat agagaggatt tatatcgacg acgttttctt ctttcatcgg ccatccttat
     8101 ccccatcacc cctaaacaga gtaaaaacgg gatgatatct gagatttccc atcgcaagaa
     8161 cgagacactc ttttgccaga tataaaagac ggaaaaagcg gtgaaaaaga ccaggttctt
     8221 ccgttcaaaa tagcgtatcg accagtagta gagaaagaac agcaatagaa aaatcgggct
     8281 tagccaaaga tcgatgccgt aggccagggg cacgatccaa acgaacgcgg ttgccgagag
     8341 catcacgatc cagtacgggg tcttgagcga aaaagagcct cccagggcta gagcaaaagg
     8401 cgatagcagc cagtgttggg acagcagaag gtcccaaaac agcgagaaaa catagacggg
     8461 ggcgatgacc aagagataga ccatcgccag ttacgcccta cggctgagcc ggtcaagaaa
     8521 accgatcata ttcttgaaga cgctctgacc ttcggatttg ttctcgtaat cgatctgcca
     8581 gagctcgtct aaaatcctag agacaccctc ggcttcgtac gcatacaaga gattgcgtcg
     8641 ttttttcgcc cgatcgaaag agtagccata aaagcgcgaa agggtcggta tgttcaggcc
     8701 ggtgtccttg gagatttctc gtaagtcttc aaagcgactc acctgttttc gatggtgcgt
     8761 gatcaagtgg gacaaacaca tcgcggcctt tatcaactgg aaaaggatca ggttttccgg
     8821 gtattcggaa aacacgggat ataggcttcg aaagacggcg gcatggtctt gttccaggac
     8881 agaaaacacg aagtcatcca ggttttccgg ttcgtaggga aagatcaacg cgttgaaaag
     8941 gtccgtttcg atttggccgt tcacgctagc ggctcggacc ttggtgatct cgttcgcgat
     9001 tctttccagc tccgggccgc accgctcgaa taggagcgca gcctgtccgg ggctcaatgt
     9061 caagccctcg cgtttcgcca aaagcacggt tttttctatc cattccgcgc gcttccacgg
     9121 ttttggcagg tcatactgaa acgattggaa cggtttgaac agagggttcg ctttcgaacc
     9181 accgtagtct atgatgaccg ataccgcttt tttctgcgct tcaacgccga tgtacctaaa
     9241 ggcattcgtt tgaacgtgga cgctcgccga ttcgaattcg ctcaggtaaa agagaaaggg
     9301 gttaccgaac agggaaggcg tcgtgatcct ttgtctgaaa ggctcccact ggtctttttt
     9361 atggagctcg acgaaaacga aggcggcttc ttgttgttgc gcgatacgcc ttgcctccgc
     9421 ttttttggta tatcgatcgt ctcccaggaa gacatagacc atagcttttc aagaccctcc
     9481 tctctcctca acgtttctcg attaggcgga caggcgcggg acgataaaag atctccagta
     9541 gacgcggaac ataattccga ccggaacggc gatcaacgat cctacgatgc cgaaaagttg
     9601 gctgcacacc agtatcagaa gaatgataac gactgggttg agttgaacga cgcctttcat
     9661 caaattaaga aacataatga atgcgacggg gtgcacgatg ctgatcaaca cgagtaaagc
     9721 aacgctcttg ccgatgccga gagaaaaaca aaggatcagc aacgggatca gttccaaagc
     9781 taccccgacg atagggataa agttcgtaaa aaagccccaa aaccccaaga tcaacgcgta
     9841 ctgcgagccg atgatgagca aaaagatgcc gattacgatt ccgacgaacg tggcgaccag
     9901 gatcagcgag gagaggtacc gtttcaattg atgatagagt tcccggagaa accgcctcgc
     9961 ttcctcggtg gtcgttttgg gaaacaagtg tagaatcgat ttcttaaagg tggggaagaa
    10021 aaacgtccag tacgcggtga ccaaaacgac gtagaatatg aaattcaaca gagtcatcga
    10081 ggatttttgc aggccggaga cgaaggactc gaaaaaagag agcaacttgg gatagatctt
    10141 gtcgatcgtg ggggaaagca attcttttgt ttcggggcgc agttgaaaga aggtctccca
    10201 tcggcgtttc ccgaaaaaat cctcgacgaa tcggaaaaaa ccgccggctt ccttcagggt
    10261 gctcggcact agaaacgcga agagaagcag cacacagctg aaaaacacga tcagcgtcaa
    10321 cgcgtgcgcc aagacgaatc gcatccgtcg cttgtgtatc agccatccgg ccaccggttc
    10381 caagacgatg atgatagcga aagcggtcag cactaccgaa aaggctaacg gcgagagata
    10441 cccgaataac aagattaacg acacgtaaaa cagcaggtag gcttcgaaaa gtaattttgg
    10501 gggtctgttc ttgtcaaaag ggttacgcat caccattccc cctgtaagac ccggaagagg
    10561 gcttcgattt ttaagatatc catacacacg gtgtagtcca gcgcctccag tttggcgagg
    10621 gtttggttga tgtctttcaa aacggcggct tcagtttggc caagaagata gcttttggag
    10681 agcttgctcc gttgggtatt ggtttcggtg atctgtcggc gtaagtctgg gagaatctcc
    10741 gaaagcacct cgtgctccaa tatcattttt cgcatagaca cttctctcat tttttcctct
    10801 agactgctta gaggctcaaa ctccggttga taatgcactc ccggaccacc caaaatgtaa
    10861 ccgaagtcgc gttcgtgaac cgtttgcaac ttcagataga gagagacgct tttttgaagg
    10921 tccctctgca tgaccatcga gtagttcagg agcgcggacc tcacgtcttc gtctcttaac
    10981 ggagaaaaca cgagcccttg ggtgtcgatc gcctcgggtt ccccttcgga tagaccggag
    11041 agcgtctgga gttgtatcaa gagatacgcg agttcgatct gctccaaccg gttttttacc
    11101 accgcgactg cgtccgaatc gtccgcgttt tcgagcgcct tccggcgctt caggtattcg
    11161 aaatagagtt cgcaggcctg tattttccga agcatcgtct cttttctcgc ctccgcgtag
    11221 gctttacgcg atgtgctttg caggtccccg aaccgatagg tcaatcccat gttaaacaga
    11281 taggctgtgg aggaagaaaa atccgtgaat acctgaaacg agctgttgaa gtgcaacgca
    11341 tcgaaaacct gcgcatcagc ggaggcgccg atgacgactt gccgattggc gagatcgtag
    11401 ccggcgccga ggtcggcgtc gacccgttga acccaaatga atctcggaag tcccatctcg
    11461 gccaggcaag aggattcaac gccttctaaa acctgtcgga tcgtataagc gtaaccgctc
    11521 caagcgaata aacacacaca gaccatagtg ccaaacgcct ttttcagaac cgctccccct
    11581 ttttcctcga gcgtctttag gataaaaacg ctctagctgt cgctctcgct catcttgaag
    11641 taattggcca tttcgaaata gccccggatc tcttcgatat gagaagtctt tcccaagacc
    11701 accatcagtt tgatgcgcgc tttttggccc gtttgctcgt gggccagaat cacgcccatt
    11761 tttttcagat aggcgcctcc cccttcgtag ctgtagatgt ccaaaacgcg ccctttgtat
    11821 gctcgggaaa ccaagacgac ggcaacctgg tgctggatag ccgtttttag gctgttgata
    11881 atcgtatccg gaacgtttcc ccggccgaat ccctctagaa cgataccctt atacccctta
    11941 tctaccgcgt agtcgatgaa ctcccctaaa tcaccggtgt aggttttcaa gagcgcgaca
    12001 tggggctcga tcttcgtcgt cgggatatgg taacggaccg tggatttccg gaagaaaaaa
    12061 acgccgtcct cgtccacgat accgagcggt ccgaatccgg gagactcgaa ggtggcgaca
    12121 ttactggtat aggtcttgtt tacctccctg gccgcgtgga tttcgtcgtt gagacacaca
    12181 acggcgccag cgcctatggc ttgcgtggag caggccaccc ggatcgagga aaaaatgttt
    12241 cggggaccat ctgtgccgag ttcgttgatg ttccgcatcg cggcggttag aacgatcggc
    12301 ttttttgtca gcaccgtgat gtccaggaaa taagcggtct cttccaatgt gtccgtgccg
    12361 tgggtcacga cgatcccgtc aacctcatcc cgatccacca gaccctggat ccgtcgggag
    12421 agctgccaca tcagctgtgg gctcatatgc gggctcggta agttggcgaa ttcgaccatc
    12481 ttgacctgcg cgagattcgt aagcccctct agttccatga tctcttgatt gccttcgggg
    12541 gtgatatcaa cgccggcggg tagcttcttc atcgcgatag tgccgccggt ggtgacgata
    12601 acgatgtttt tcatttcttc aggcctcctt tttctgggtc ccgaaaaaca ggatccgtct
    12661 gactaacgcc cagtgacgag agtgtttgcg catacaggcg ctctcggttt actcgggtga
    12721 gttcgagcac cgcgtccggg cgtaagcctt tgacttttct aagaaaaggg tagtcgccca
    12781 ggcggatcac ggcgatggcg aatgggcaac gtgtcaaggc cgacaagatc gactgctgca
    12841 gacgatcatc cagggtttcc aaaaaaccga tctcatcgat gtacacccct tcggtctggg
    12901 ggtcgatcga ctctagcagc gcggccatac gcccgaaggc ttctgtgtcg ggtaggatgc
    12961 gcctgtaggg aagcaggttt tgcacggcga ccctaaaggg cttctcatcg aagccgaccg
    13021 gctggcagat caagtggcgc gccccttccg gtttgtctat gaaaacggtt tcgtatcctt
    13081 tgaaccgggc gccctcggac agggtaagtc tttgagaaag atgcctcgcg aaagtcgatt
    13141 tcccggctcc cctctccccc gtaatgagca taagcatcaa ccgcgcttcg tcgactttca
    13201 accgatactt tcccgaacgt acgtgtcgag gcgcgcgagc acgtccggca acttttccgg
    13261 ccgctttccc ccggcgaagg cgaaatcggg tttgccgccc ccgccgcctc ccaactccgt
    13321 cgcgagtcgc cgcgcgatat tcccggcgtg aaagggattt tgaacgaacg gtgtcgcgac
    13381 cttcacgcag atatccacca tcccctcccg ctcgttgaag acgatggcca tccccgtctg
    13441 gtgttgctct agggcgaggt cggtcacaga tcgaacggat tctttctgca acccattaag
    13501 ccgaatgacc gtgtaggaag cgtcgccaag ctgatggacc gccctttcgg gtcggccgat
    13561 ttctttcagg ctccactttt cctgaaggtt ccggatcgtt ttttcggctt ctttttgcat
    13621 ttcttgcatc ttttttatcg tttgcgccag ggattccgat tgcgagccag agacctggca
    13681 cgcttccaac agcgttgcgt tcagttttcg aacgtaggcg agagaggtta ggccggtgat
    13741 cgcttcaatc cgcctcatgc cggccgatat agcggtttcg ccaagtattt taaagagccc
    13801 gatctccccg gtattctgga cgtgcgttcc gccgcacagc tccaagctat gcgttccgac
    13861 tcgaacgacc cgtacggttt ctttgtactt ttcgtcgaac agggccatcg cgcccatggc
    13921 cttcgcttcc tggatcgaac gactctcgat ggagaccgga atggccatta aaacgcgctc
    13981 gttgacccag tcctctatcg cttccacttg attcgcacgg atcttttcat aatgcgagaa
    14041 atcgaagcgc aggcggtccg gctcgacgag cgaccccgct tgctgaacgt gatctccaag
    14101 gacagccctc aaagctgcgt gcagcagatg cgtcgccgta tggtttcgag cgacggcccg
    14161 gcgcctgggc aggtcgaccg agagaaggac cctctccccg gtcctcaaaa caccggtttt
    14221 tacgatgatt cggtgggaca ccaactggtt gaatgggtta aagacgtagg tcacatcccc
    14281 cgcgcctgat ggcccactga aagaacctat gtcactggcc tggccccctt tttcgctaaa
    14341 aaacggggtg ctctcgacca ccacctcgcc ttcctccccc gcatgaagct ccggaaccgt
    14401 ttgtccctct cgaaccagat aaagcacgtt ggagtggccc tcgttccgat cgtacccgat
    14461 aaacggagtc acggggtatt ccgagccgat agattcatag atttcttgca ttttgaggaa
    14521 ctctttgtct ccacgcgcag cgcgggcgct ttcccgctgc ttctccatca ggtcgcggta
    14581 accgtccata tcgatgacca gccctctttc ttgtgccact tccccagtga cttcgatcgg
    14641 gaaccccaaa gtatcgtaaa gccggaaggc gtcgtctgcg gcgatccgct tggaatgagc
    14701 cgtcaaagtg tcgagcatcg ccatcccttg ctccagggtt ttcaagaatt tttcttcttc
    14761 taggcggatg atcttcgtta ccatcggcac tttgttcacg atttcggggt agggttctcc
    14821 cataacggaa acgaccggtt ccacaaggcg gtacaagaac gggtttcgct gtccgtagag
    14881 ccagccgaaa cgcatcgcgc ggcgaatgag gcgttttaaa acgtagccgc gaccgtcgtt
    14941 cgagggaaga atgccgtcgg taatcatgaa gaccgctgac cgtatgtggt ccgccacgac
    15001 ccgcaacgcg acgttactcg cttcgctgct gccatagcgc agaccgagga cggtttcgct
    15061 ctgtcgcacg atcggggtga aaaggtcgga ttcgaagttg gaagagaccc cctgcatgat
    15121 ggcggcaagc cgttccaaac cgaggccagt gtcgatattt ttacgggaca agggggtcag
    15181 gttgcctttt tcgtcttgat tgaactcggt gaagacgaga ttgtagaact ccaagaaacg
    15241 gccgcaatcg caaccgggcg cgcagcccct cgggtccggg cagcccgatt tgggccccat
    15301 atcgtaaaaa atctccgaat ccgggccgca aggtcccgtg ggtccggcgg gcccccaaaa
    15361 gttgtcctct ttccccaatc gggtgatctt tcggggtggg agaccgatat ggtgaagcca
    15421 aagatcataa gcttcctggt cttctctata tacggaaacg tatatctttt cctcaggcag
    15481 cttcaagacg tcgatgacga actcccaagc ccactcgatc gcctcgcgtt tgaagtagtc
    15541 cccgaaggag aagttcccga gcatctcaaa gaacgtgtgg tggcgcgggg tgcggccgac
    15601 ctgttcgata tcgttggttc ggacgcactt ctggcaagtc gtgacacgcg tgtaagtcgg
    15661 ttccaccttt ccccagaaga tcggtttgaa cggaaccatc ccggcaaccg tgaagagcag
    15721 ttgggggtct tccggaatca gagaagcgct gggtagccgg atatgctttt tgccttcgta
    15781 aaaggagcag aataattctc taatcgtatg tcctgacaat ttttccatca tcgtaatcca
    15841 cctttatttg aaaaatacga gtaatagcgc gaacgttggg ataaagaaga ccatcgagtc
    15901 gattcggtcc aaccagcctc cgtggcctgg gaggattttc ccgatgtctt tcaatgcgta
    15961 agacctttta agggccgatt cgaacaaatc tccgaaagtg gcgctcatcc acaatccaat
    16021 cgacaataac ccgatctgcc ataaggtgaa gatcggctgt ttccagaaca actccaagag
    16081 gcggattacc aaccacaaac cgaaaaaccc gcctaccagt ccgcctatgg acccttccac
    16141 cgtcttcttc ggggagattg tcggcatcaa agggtgtttg ccgtaacgaa tcccgacgaa
    16201 aaaagccatc gagtcgaata tccatgtgga ggtgaccgcc aacagggcat aaggccagcc
    16261 gtatttccag taaatgtaga aaccgagcgt atggacgaaa ggcagataaa acacaccgaa
    16321 aacggcgtct acgatacggt cctttatagt ggccacttcg cgatggataa gcaattcgac
    16381 aacacagatc aacgggatct gtaaggcgta aaggctcacg attgcgccca ttccctgttc
    16441 cggaagaagt aaaatgaaaa gcccggatag gcaggtcaga gtgggcaaca ggaccgacaa
    16501 ccacaggcgc tcgaccgcat ttggggtttt cgccaagatc aagcgaacga actcgtatcc
    16561 tccaattgcc gtgcttaaaa ctgccagcgc taaaaaggta tggatgttga agaacatcca
    16621 agagatcgcc aagccaccga taatcgcgga ggtccaacgc gccttgatag agaacctgtc
    16681 tttctttaag tccttaggag ggttgttcat ctaacccgcc aaaccttctc ttacgcccag
    16741 aatactcttt taaagcagca tcgaaacatc gttcgtcgaa gtccggccat aaggtgtctg
    16801 tgaagtacag ctccgaatag gcggattgcc acaacaagca gttgctgatc cgcttttctc
    16861 ccgaagtccg aacgatgaga tcgggggcgg gaacgtccgg aaggtagaga tagcgataga
    16921 agccctccga gagggtgtcc aatatctctt ctttttggtc aggaggacgg gtgttgagtt
    16981 cctcgtgaag cttcctgaac ccgtcgcaaa tctcctggcg ccctccgtaa ttaacggcca
    17041 ggatcatctg cagccgatcg caatgaagag accgcgcttc gatttttcgg atcaactccc
    17101 gcaaagcggg gctcaaccct ggcatccgtc ccataaaccg gatgcgcacc cctttattga
    17161 tcatctcttc cgtatgctcc aacaggtact cggagagaag ctgaaaaaga aaatgcacct
    17221 cactttcggg gcgtttccag ttttctgtgg aaaaggcgaa tagggtgaca taccggattc
    17281 cgtgatcggc gcataacctg accgtcgctt cggccacctg cgcgcctttc gcgtgcccct
    17341 cggaccgttc taaccctcgt cgtttcgccc accgtccgtt gccgtccata atgaaggcga
    17401 tatgggtagg gttacccatc agacctccat gatctcttct tcttttcgtt tgaacaggtc
    17461 gtccattttt tcgacatact cgtcggtgac tttctgaacc tccgcttcgt aggccttcgc
    17521 gtcatcctcg ggtatatcgc ctttcttctg ctcttctttg atgaccttga tggcatcgcg
    17581 gcggatattc cgtacagaaa tcttcccgtt ttctatcttt tccttgacca ccttcacgag
    17641 gttcttcttt tgttctccgg agggaggcgg gaagttcagg cggatattgt tgccgtcgct
    17701 catcggcgtc aatcccaacg aggaggcgag gatcgccttt tcaatcagct tcagcgtggc
    17761 tttgtcccag ggcttgatga tcagcgcccg ttcatcgatc acgatagagg ccagctgcgt
    17821 caccggcgtc ggcgccccgt aataatcgac ggagatatcg tcgagcatcg ccggcgcagg
    17881 tcttccggta cggatgtgct tcagctcttc gtcaaccact tgcacggttt tttgcatctt
    17941 ttcctttgct tcggtggcgt atttgttcat cttctatcct cctaacgtac cccgttcttc
    18001 tcttttagtg ggtatcccaa ccatagagag tttcgcatta ttatatcata acgatccgtc
    18061 gggaccttgc ccgcgtgtgt gtcaagttat ggggtgacaa aactaagagg ttcctcgcag
    18121 gtaagtgatt tttacgaccc cgcgatccgc attttgccct ttctacgccg caatgggctt
    18181 gtgcaaaagc gtatcgcgcc gtcgcgaaaa gaagaggtgc ttacgccccg atgactacgt
    18241 ggaataaaaa tcgtgtgatc gattctggcc ttgtttcttg agtactcccc ataacttgaa
    18301 ccacaccagg atttagggaa agagaacacg gagagtgtgg cacgggtttc tcagaacgcc
    18361 gaactttagt tcggcatatc cttataagag atctgcggtt tcccacagtg ttcacggagg
    18421 acatagaggg gagaaaaaag ggaaaaataa aacccgtccg ggctttggcc cgccaggctt
    18481 ttcatcgggg cgaggcgttc tcgggaagtc gacggcgtgt acgggcgcca tcgtttatgt
    18541 tatactccat tacgaggaca cataaggtat ccggagttcg ccaataggac cgggtcaggc
    18601 gtttttatta ggcggtacag cgccgggagt caagatgctc atttcttttc taaagcacca
    18661 ggccagatat aaaatacatc cggagatcga cccgctgata gaatcgatct cggcggttca
    18721 ccaagacacg ggcttatact tgcatatccc gttttgcaag cacttttgta ctttttgtcc
    18781 ttaccataag ttccgatacg atgaaaacat gctccggact tacactgaag cggtttttcg
    18841 agaatttggc ctctatccgc ctagggaata cgactcccta tacgtcggcg ggggaacccc
    18901 gacggttcac cagggcttgt tgctcgagtt aattcggttt ttccgcccga tcattcggcg
    18961 tgaaatcggc gtggagctac atccgttgga cgccacgggg gaattgctcg agttgatgga
    19021 aaccgaaggg gtacaatacc tcagcttggg gatccaatcg atgcacgatg aggtattgcg
    19081 tcattttggt aggaaccact cggcgcggga caaccatcgc gcgttaagcc aggttatgaa
    19141 cgccggctac aagttggtgg acgtggacct tgtcttcgac ctcgccgcct tctcttcgga
    19201 ggtcgttttg aaggacttcg agaccgtctg cgcctacctt ccccatcaaa tctctatcta
    19261 cccgatgatg cggttttcct tcgcacccta tcggactaaa aatcaacccg cacgagagtt
    19321 cgagaccttt ttgcagatcg acaagctcgc cctgcgaatg ggctaccatc gggacatgct
    19381 ctggacctat cggcgatcgg gggtggccgg gcggtattcg tcggtcacca ggcccttttt
    19441 tcggggaata ggcatcagcg cctcctcctt caacggggag gggttttatg tcaacacctt
    19501 cgactacgac cgctacctta cttgcatcgc gaacgctcgc ctgccggttt cgaaacgcta
    19561 tcggctctcg gactgggaga acgccttgtt ctatctcttc tggtcgctct atcgcaatga
    19621 actgaatctc gcgctgtggt gcgactactt tcctcaacag aaagcccggg gtcgtttgct
    19681 gtttcgctac cttcggctgt taggctattt agagccgatc tctccggagt tgtgggggtt
    19741 gacgcaacac ggtcgcaaag ccttccatcg gctggaagaa tggctgacct atgcgatgat
    19801 agaaccgatc tggaacgact acgccacgac accgagggcg cgcgattccg accagcctca
    19861 gcctgcccga gggccttaat cggtttgagg aagaatcgcg ggaaaccgcg accgttcgcg
    19921 tcaatcgcgt gtcagaaagg atttggaaaa agagaccgcc ccttcgatct cttccgagtt
    19981 gggatgacct aagaagaaga acaaaaaact gcccttgcaa caatactctt caggagcgac
    20041 ctgtatcccc cggctttcga gtgcggtgcg gacggcggct tgcttcagac ttccgttggc
    20101 gctggacgtc accaggaccg ctttttttat ctgtttcggg ctgagccctt tgacgaactc
    20161 gagcatcata gggtcgcttt gtttgccgta aaggccgcca acgatgaata acaggtcgac
    20221 ttctccgatg gccggtcggg ttttaatatc gagggccgag agcgataatg ccgaagcgat
    20281 cgccgaggcg atctttttcg agtgtccggt tttcgtaagg taaacgactg cgtggttcac
    20341 ggagcccctc ctttttccgt ttgatcaaaa aacggaccta tacgctatta tacgctaaaa
    20401 gcaaattggg gacgccaaaa tcgaatcaag gaccttaacc cccgtttgac gaccctatag
    20461 gttcgcggtc cgcaaggcgc gtagggattc cggtttcatt tttaaacggg cctgttgatg
    20521 gggtataata acacgaaatc cggttcaggg atcgaaatcg tgtatgatag ggttagtatc
    20581 agaaaagagg ggttcacgat gaaaatagcc gtggcgggcg gggtgaccgg cggtcatatc
    20641 tatcccgcgg tggccgttct agagtatttt atgaaacagt atccggctgt ggaggttctg
    20701 tatttcgcca cgccgcatgg gttggaaaac cgggagatcg tacgtctttt cccgaaagcc
    20761 cgaatcgtca agctcgaaat acgcggggta ctccgccctt tcttcactcc gaagaaccta
    20821 tctatccttt ataaagcggc gcgttccgtc tacgtctgca agaaagaact ggcctcgttc
    20881 agaccagatt tcagtttcat caccggaggg tacgtcgccg ttccggtcgg ttgggcctcc
    20941 cataagttac ggattccgac ctttttacac gaacaaaacg caattgccgg tttagcaaac
    21001 aaattgatca gccggggctc cgagcgtatc tttatctctt ttccggaaag cgctcgggat
    21061 tttaaactca agagcgccca tcgcattctg cataccggga acccggtccg tatcgccgag
    21121 aacgtcgacc gaggggtctt ccatcgctac gggatcaacc cgaacaaaaa gatcgtgttg
    21181 gtggcgggag gaagcaaagg atccgatttc gtgaacgggt tgatgcttca gctctatgag
    21241 aaatggcgcg ggaacaacag tcaggtggag tttttgcatt cttgcgggga agccgccttt
    21301 cttcagaaga tcagcgcttt cccctttgtg aaagcggctc ctttcatcca agacatgcac
    21361 cgctacatcg cctgcgcgga cgcgatggtc tctcggggcg gcgcgaccac agtagcggag
    21421 atcacctact atggggtccc ctctctcatc ataccgtggt ccggatcagc tgaaaatcat
    21481 caattgatca acgcgatttc tctggaaaaa cacggggccg ctttctatct ggaagagagg
    21541 gaggctaccg tggcgctcct agaaaagaag ctgacccttc tcttagaaaa gaagctatcg
    21601 caaaatatgc gtttcgcgct gaagcggctg aggcccaaga tgaatccggc gaaaaaaatt
    21661 ttccacgaga tcctcagcgc gctgcctgag tccttgagaa gcgaggccga tccacgctag
    21721 gccctgtttc taccgaaagc caagcttgtt attatactca atgcgccgcg aagatatcgc
    21781 ctaatcgagc gcgcggtaaa atgaaagggg gaaatatgga agaattgggt ttttgtggta
    21841 tcgactgcac ggtgtgtccg gcctaccagg cgacgatccg aaacgacgcc gctatgcgcg
    21901 aaaaaacggc tgctgaatgg acgaaaatgt tcgggaacga tttcgagatg gaagacatcg
    21961 tctgctacgg atgtcaggag cgcgagaagc ctgtctttgg gtactgcgac aagtgccgag
    22021 ttcggctgtg cgcgctcgag cgcggtgtgc tcacctgcgc cgaatgttcc gactacgtgt
    22081 gtgaaaaact ggcagaattc tacgagcagg ttccgcaaga aaaagcgagg atggataaac
    22141 gtcacgaagt cttcttacga agtcaacggc cggaatgacc ggactctctt tccgttgtcc
    22201 tgaggtttgc ctcttcccag tgtgggtata atatcggggt acaccaaatt gggaacgccg
    22261 aactttagtt cggtatgttt ttttgctatt cagtaagctc tcgagagccc ttaaacgctt
    22321 acgctgcgat ggccgcgcga aagcgcatcg cgccgtcgcg aaaagaagaa gcgcttacgt
    22381 cgcgctgacc gtgtggggtt atagcggggt aaccagcggg cagacttcgg aagcctgcga
    22441 agtcccgcga tagatcgttg cataaccctt ttctatgccc aaatcgaccg cctggcggtc
    22501 tctttctatt cctcgcagga aacatcg
//
LOCUS       DAOPXN010000012        22379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2319_length_22379_cov_5.453817, whole genome shotgun sequence.
ACCESSION   DAOPXN010000012 DAOPXN010000000
VERSION     DAOPXN010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 22379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22379
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2319_length_22379_cov_5.453817"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="PKM49_01360"
     CDS             complement(<1..1760)
                     /locus_tag="PKM49_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY81494.1"
                     /translation="MKIRSVKTKLLLLFIPVSVVLLVVAAIIIGSIARNVTFALAQSS
                     AEETLNATVRIVEEWLEGLKSTMHTLGLTEEIQSIDRGRYTPLLVSLKKEAGGLFELV
                     FFVDPSGKAMTDGGEIYDLNDRDYFREVIHKGKPYAVSNAVVSKQSNNPVFTVVVQAK
                     DPKGKVIGAVGATVTLKALSEKVSSIRLGKEGYTFLTDGTGLIIAHPEAKFVMDLNLL
                     ESAKVGYKGLEEVGRELLSGKSGLRKMTKPSGSKEIALYEPVGGTMNWGLTAVISESE
                     VGEKSIFLLIIVLVAFAIIVGATVLISLLTGTLISKPLKELARRVDTFGKGDLTVSFE
                     AKGSDEIAQMAQALDTMGRELRDSVVSILHASNEIKSASSNLAAVSEEELALSEEVAA
                     QSHAADRQLQNTAASIEEVNSGVEEVAASAQNVSHTAQSLSAQNEKTAERSREGGQMI
                     SAVVKRIDETTKQTLLTAGKVQMLAKHAKNVGEIVETISSIAEQTNLLALNAAIEAAR
                     AGEAGRGFAVVADEIRKLAEESKKATHNIGTILKEVGRDATESNQATEQTVELIKEVN
                     EEARAVEKQFGQILTMVDDTS"
     gene            complement(2698..3588)
                     /locus_tag="PKM49_01365"
     CDS             complement(2698..3588)
                     /locus_tag="PKM49_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY81495.1"
                     /translation="MTKHIAIMSGKGGTGKTTVAASFAYLAQRPPTTRIVIADADVDA
                     ANLHLLLGFRCENQERYFGGKKAIIEPTRCVGCGLCEPVCRFFAIHMENDLAHINSLT
                     CEGCKACVFACPHDAITMEAMQSGWLRDGSAIGIPFADGELFPGEETSGKLVTEVRKL
                     ADGKALKEAAASVLVDGAPGIGCSAIASITGARALIIVTEPSKSGWHDLERMMETAKH
                     FNVHYSVIINKYDISEAYTQFVKENLKRRNADIIGLIPFDERVVEATRKGVPVVQIGG
                     AASEAITRIWDTTIRPLLLE"
     gene            complement(3585..4439)
                     /locus_tag="PKM49_01370"
     CDS             complement(3585..4439)
                     /locus_tag="PKM49_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY81496.1"
                     /translation="MRSLTVVSGKGGTGKTLFSTNLAWLFHQKGVATQLLDVDAEEPN
                     ASLFVQFIPIRTQPVEKLIPHIDESLCDRCGLCMKECQFSAITIAPKKTAVLAPLCHG
                     CGLCARLCPQKAITELPKTIGNIRLGKTQEGFSFGEGILNIGEPSAVPVIRQLKNLAP
                     KDAQIRILDAPPGASCSVVESLSGSDSALLVTEPTPFGLHDLRAAIAVVSEMKIPFKV
                     VINRFDAGSSEYVEKIRHLDAEVIATLPFDRKIASVYAEGKLVVKELPKYLPVFERII
                     EAIGRGFA"
     gene            complement(4432..4749)
                     /locus_tag="PKM49_01375"
     CDS             complement(4432..4749)
                     /locus_tag="PKM49_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNY81497.1"
                     /translation="MKVLVTAEGDTAQASVCGRLARAPYFLIYDTQTEQWTSYANPYQ
                     QEHGMGSRFAQFAAQKGVQVVLGAAPGPNAKAALDTLGIKFALATGVTAQEALSQYLA
                     THA"
     gene            complement(4778..5029)
                     /locus_tag="PKM49_01380"
     CDS             complement(4778..5029)
                     /locus_tag="PKM49_01380"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNY81498.1"
                     /translation="MPYGDGTGPVWGRGIGCGVPRGYRARFFRRGFGRGLGFRRFWGE
                     PFEGDYVPMTPETEREILEQEKQYLTQRLAYIQERLGKE"
     gene            complement(5044..5415)
                     /locus_tag="PKM49_01385"
     CDS             complement(5044..5415)
                     /locus_tag="PKM49_01385"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNY81499.1"
                     /translation="MRGGHRGGQGRFGRGRVLFEGYILLLLYEKPCHGYELAKQLTDL
                     GIHLPGVGAMGHLYRTLMVFEESGWIRSYWKTEDIGPAKKVYEITNGGKDMLMGISKD
                     LEHTRYQIETFQKRLQAKLST"
     assembly_gap    6506..6605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7021..7962)
                     /locus_tag="PKM49_01390"
     CDS             complement(7021..7962)
                     /locus_tag="PKM49_01390"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81500.1"
                     /translation="MLIFGPVTSRRLGQSLGINHIPAKTCSYACVYCQLGRTYPMQVK
                     RESFFSPEEIDRELTQRLRQLRETNEPVDWVTFVPSGEPTLDLCLKKEIENTKAHGLK
                     VAVISNSSLMGMEEVRDALMEADWVSLKVDAGNDSMWRKVNRPYGSLNYEEMLKGIVT
                     FSRRYRGILVTESMLIKGMNDNESEIADITRALSSIRPKKSYLMVPTRPPAESGVERP
                     NAETLGQIVKQVRGVCPHEIECITGDENEERVFVTDAVVYDILAITSVHPVRESTLRL
                     LLERKRVDFSLIEELVAQKKLLSFFYGGTTFYRRNLE"
     gene            8163..9431
                     /locus_tag="PKM49_01395"
     CDS             8163..9431
                     /locus_tag="PKM49_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY81501.1"
                     /translation="MKLKGMFLVGILLICAVFAATIELTVWTHEDPNRTLLEERYIEE
                     FQKLYPEVSVKRVTYPSAKIKETVLTAFSAGKGPDIFNMEVQDEYPYIVNRRVAPVNP
                     AALGYKNVEEIYAAYLEGTLDPVSYQGKLYGVPLEITNWCIFVNTRYTKALGLDFENN
                     YPKTWEELMEVSDKLTLRNGEIITRRGFDFRYPYYLTIFTPMVEQMGGYLLNKEGTEA
                     IINPQAWVEVLTFMKEWGPSGRNLGSPTYTAARKNWNKDDGSIVMIESGLYQILRLKA
                     ENPELYESKEWMVIPWPVFENAVNHVKGNYYGHYYVVNAQSSPEKQEWAWKLIAYMLK
                     HTGEYFEEVGLIQPTYELMESEVFKNFPYANVFMEDMSQAGCVLLHQAGPRLEQLIRE
                     AVESVMLSGVTPENAVKTLKAKATEVLEDY"
     gene            9509..10408
                     /locus_tag="PKM49_01400"
     CDS             9509..10408
                     /locus_tag="PKM49_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81502.1"
                     /translation="MKSGIEKKKARWGWFFVLPAIIYFSIFSFFPIINAFRTSLYRWN
                     LLSLNPPRFVWFENYIRLFQSEAFWNSVKATAIFTVGTFSSLLIGGLALSILIFSRKR
                     FRTFFQMALYSPAVLSTVVAATIWLLILDPRGLGNSVLNAVLGTSGKDYQWFANPDMS
                     RISTIIIYFWKYIGHFTILFIAGLSSIPYTIHEAAIIDGAGPWKKYWRITFPLLKPTV
                     VLVCVLSLIQCMKSFSTQYLFVQSGAPRAPIDVLTLSIYDTAIRDHQIGRASAMSIIL
                     FLTLILFSWAQLRLSRSEEVSFQ"
     gene            10405..11271
                     /locus_tag="PKM49_01405"
     CDS             10405..11271
                     /locus_tag="PKM49_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006188005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY81503.1"
                     /translation="MSRADNRLKNTARSLREIVFDLILLVVLAIVLVPLVFMFTASFM
                     ESKEVLTMPYRWIPSSFHWENYVQAIQGNDMKYFYARNILNSLIVAITVTLTNCVFAA
                     MTGFGLAKYRFFGKRAVLILVMATMMIPFETIMIPLYMLVNNFGWQDSYMGLIMPFLV
                     NAFGIFMMRQFLLTFPEDLLDAARIDGLNELSIFIRVVIPNSIPAMTALGILTFRSQW
                     DSMLWPLLVTQSEEMKTIPLYITKFTTEKYTNEGALLAAAVIASIPLFVVFFSLAKYF
                     IQGSSVFSGTKG"
     gene            11323..12360
                     /locus_tag="PKM49_01410"
     CDS             11323..12360
                     /locus_tag="PKM49_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81504.1"
                     /translation="MHIEDWTPYCFIIRDLLYDGSWETMKKDAEGNTFLEPLIQQAQD
                     LEKLAGESLPGSLFLPTGAVEIAHFFSHHQWEMSRLVLSKMDGLYELASAALEEENYE
                     EARALAELLEGIKQENAYSEEIYGSLAVLKGDYAQGIQHLEMAIRLDPRFVPALSSLS
                     LAYFNTQKYEKAIETSQHILENRPEDLLAYITIVDSYINLGLADQALETLRRMNQAIP
                     SNVTGKIQLYRMLREHRRDAEADAVRKDILTIYPSQPHELEIFVTLLFETGNFEKAEL
                     EINDYLARNPHHTYLKILLIVPLIKKGFIQEARRIVEEFKEQKVWYYFGKKETLGSFM
                     SESEQKGCGLI"
     gene            12357..12968
                     /gene="udk"
                     /locus_tag="PKM49_01415"
     CDS             12357..12968
                     /gene="udk"
                     /locus_tag="PKM49_01415"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HNY81505.1"
                     /translation="MMLAIAGGSGSGKTTVAIRLSERLKEHCALLSMDSYYVDLPPGI
                     NPGDFNFDAPSAFDFTLLKKDLMELKASRPIRIPSYSFITYRREPGLFTEMKPSSIVI
                     VEGILVLYDPELRKLFDFSIYVETQDDERLLRRIERDMVERQRSIESIIRQYRRFVAP
                     AYRSFVEPQKHYADIILPSGGYNEPGMEIIYNALVQKMRQSRE"
     gene            complement(13037..13492)
                     /locus_tag="PKM49_01420"
     CDS             complement(13037..13492)
                     /locus_tag="PKM49_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNY81506.1"
                     /translation="MSIMIRKAREDDFYKTFVDMQREIDNVFSAFLRGGSPMAPKGCK
                     IPSVDVYENEKEFVFEFELPGLRKEDIKISVEENVLSVESVRQETKEEKDQSYHIVER
                     RTGCFKRQFTLPENAKPEDISAKYENGLLELRLPKKEKSKPRSIEVKVE"
     gene            complement(13700..15868)
                     /locus_tag="PKM49_01425"
     CDS             complement(13700..15868)
                     /locus_tag="PKM49_01425"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNY81507.1"
                     /translation="MTAVIILIAVVGSLALAAVNFFGVKKLDPGLPKMVDIADAIKEG
                     ADAFLRHEYKVISMIAIVIVALLWLSVSWYTGVAFLIGALMSASAAWVGMKIAVIANV
                     RVSNTARTTKSLGKTLKVAFRGGSVMGLCVGGFALLGLWIVYVVFGEWMGQMHIGQIR
                     IVTNWMGVSFIPFTMTVSGYALGCSVIAMFFRVGGGIFTKAADMGADLVGKTEAHIPE
                     DDPRNPATIADNVGDNVGDVAGLGADLLESFVGALISGVVLSSYMYYTTMTSASPLPE
                     ALVTKLMTYPVLFASFGIISCILGILFLLVKKVSDHPRMELNYSTWVSAGSTIVINGV
                     FSYFYFKDEAVWTAGFRFGPLSAWYAAILGIVSGIAIGLIAEYYTSYDYRPTQRVGRS
                     SLEGTALTITEGMALGMKSTALPVIVLGLAIIAADYFAGLYGVALAAIGMLSFVSATV
                     SVDTYGPIADNAGGICEMSELEHEVRAITDQLDSVGNTTAAIGKGFAIGSAAFAALSL
                     FASFLYSQAQPTDTSGYALILNMVNTLTLSGIIIGAALPYFFSGVLIEAVTKAARHMV
                     QEVRRQFKEQPGILTGEVRPDYKTCIQISSAGAVREMVFPALLAVTTPIVSGFLLGPE
                     FVGGVLLGATVSAIMLAIYTANAGGAWDNGKKYLESGNVPGIKKDSEAHAAAIIGDTV
                     GDPLKDTVGPSLDILIKIMAVVSLIAVSIFQNNNLLELFK"
     gene            complement(15865..17388)
                     /locus_tag="PKM49_01430"
     CDS             complement(15865..17388)
                     /locus_tag="PKM49_01430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNY81508.1"
                     /translation="MYLFNALYWDSSVKDYRAGNLTVRDGKIADIDVPGEGLFEKREP
                     LSGPSRDLSGHFLLPGFVDSHAHLLGIGLNSVFPSLSGLSSISALTEVVRSVKHPVVW
                     LRGWDEGLLGVFPDRQVIDRIDSERPVVLTRKCGHCACANSQAVEAFRLERWDEVDGS
                     NIGRGILKERALEAIPTGSLFTETTLTQALRDASVRCLSCGVTTIHSDDLGRVDYRTL
                     RALLLNHRGVRIYEKMLPQKESDFSEWMERPQEYFGDFGDHLRIRAVKLLLDGSFGAR
                     TAYLNAPYEGETDYRGVVYCAPEELDRFVKGCEKNGLSLCVHVIGDAALEIALRSFER
                     RAPSDNPLRHRLIHLQLASRDQIRRIKRQNLWVSLQPIFYDSDVLLAPKVLGEERYRT
                     LGYPFTALYEEGVPIALSTDAPVETVDPWPNIRAALRFFPIREVFQFYTFRSAQSGFD
                     EERVGNIRKGGCADALLFDKDPFTLNFPELERLRPVDVLFNGKWLSDNDTVYGGGFL"
     gene            complement(17477..18619)
                     /locus_tag="PKM49_01435"
     CDS             complement(17477..18619)
                     /locus_tag="PKM49_01435"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY81509.1"
                     /translation="MKHVLWVLIAILLGNVVLFPQSYKDESMSLFFAGQNAYNQGNYV
                     DAEQYLSRALQLDPDLEAKTPNAKFMLGVSAFKNQNYSIAKVNLSLYRDNPIAVDLLN
                     KISDIEGKMGTGFYYFTPIQNTAPVPETTPLSTMSQTNSAGQKSLSIPMIVGIVFAVT
                     LSLSLLMELKFAVFSRLAVRLVLAKNAIPVTVSPSHPEISQISVSSKNDEAELLLLTD
                     TPFEEKIDIQKMASAEIEDIARFFTENPEIDVEKRATGDVALTEEEIAQIAKNEILQA
                     AKSEATAGETVKDAAAGADTIFDELDVSKVIQLAQKMISEAQSAHPQKAEEAIEWKSA
                     EAFSEDKRKNIDYFEDVDEVEEEDLQRFLDLFFARETQAQELSPRY"
     gene            complement(18616..21537)
                     /locus_tag="PKM49_01440"
     CDS             complement(18616..21537)
                     /locus_tag="PKM49_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81510.1"
                     /translation="MANEEKRKKPKKTPKERPPKASKEPKKAKEKGVSGGFKLPSVLK
                     QPIDFLLSHRLLALVILVALGVLVAIFALLAIKQRGVSKATRASVQSEAKAEITDFSI
                     VIPPSAFPYTKDFTIRLIRSGDPLYALKSLGRFTGNLYELLPVDGRDDLALVPMLFRY
                     VFPSEFYFGSGFNNIELYYIDDPKNPIAQPFSGAEVKIINDTIVVEARSYHGSVIGIR
                     VSNPDKVEWGLQKKIEKRETLKPHLLIVPGVDRNFLGFLPNTISNVNPQGNNIWEVAF
                     PDRTIWSYSYPLVETRSMDYVSEATNFFEKTLRTSYIAFEASKLAQALQNYGKPMDII
                     AHGVGGLITRYAIENYPVTNVRKVVLISTPNGGTNIVNPSFLNLLYGKEKAVLAGLYG
                     LEEDTIRFIEKNNLSYLEKVNAYAAEMLPDSKIVEELAQSIRTDIEYAFIGGSSPGFQ
                     DDIERSQLAKFYPELTATKGDGVVSIYSALLPTIENPKGLKNLYGKVFDFSFYENYVQ
                     KETLLFIKQFLEEGIEEVAVPKYQDDLYREWSYEYPELVSTGTQALTGPLSLFQFSTI
                     GTQTYAQATTVSLPTPVSTAGVDIDSWKSSMDKDSSVGEAFSPRATRTESSIPSTVEA
                     TSAVRVEQETTGQPTEVRLVSTLPVIEGATTTKTATVKENGVATPSSTGTTTGLHRYV
                     PGHQDYHQLALPENFYLSNRLVHYPSFRIPLPDVQSPYGFVANGLGDFIFDAEGVIRI
                     VNGLPTRIFKGEVEAFALDNGELAFFSEEILYHYVKTHGLTKAYPGIRGKVRSMAFTG
                     DLQYYLVDNGGLILETPTSSFRIPGMTGKIILYGVYPYILTDIGLYLYDGKEIVEIYK
                     PIKPNEHFLDGLLKQSRLFILTSAYRLVALSLDGKIVNEASSNQIGGFKLLSTDNAVY
                     AVGRNKVAGLSLSPKPSEGSYFDLKDYGEVIDAQIRNAEMWVLTSENGQFGLRKIGLI
                     P"
     gene            complement(21592..>22379)
                     /locus_tag="PKM49_01445"
     CDS             complement(21592..>22379)
                     /locus_tag="PKM49_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNY81511.1"
                     /translation="FWEEIIRYPFLSGGKRFRPQLCYYSAEAFGASESDAYRLGAATE
                     ILHTASLVHDDLPEIDDDDYRRGRLATHKRYNQGLAVICADELFFLSVKTLLPLKNDL
                     LIDVFLSVAMDLAKGEAYDIYYEKTGLSLSEKELTAMYLLKTGGLIRFAITAPAILKQ
                     IPAKALAALDESAGKIGLAFQMYDDIKDETGHFEELGKTPQKDLKSGKQTILRLKTLQ
                     EAEEQADRLWREATEEIGRHSSSSQANPLVCFLNESRELIRRK"
BASE COUNT         5253 a         5960 c         5416 g         5650 t
ORIGIN      
        1 gatgtgtcgt ccaccatcgt cagtatctgt ccaaattgct tctctaccgc tcgcgcttcc
       61 tcgtttactt cttttatcaa ttccaccgtt tgctccgttg cctgattcga ttccgtcgcg
      121 tcgcgcccca cctctttgag gatggtccct atgttgtgcg tcgccttttt gctctcctcg
      181 gccaactttc ggatttcgtc cgccacaacc gcgaaccctc tgcccgcttc tcccgctctc
      241 gccgcttcta tagctgcgtt tagtgcaagc aggttcgttt gctccgcaat agaggagatc
      301 gtttctacga tctctcccac gttcttcgcg tgtttggcca acatctgaac ttttccggcg
      361 gtcaacaagg tctgtttcgt cgtctcatct atccgtttca ccacggcgga gatcatctga
      421 ccgccttccc gagaacgctc ggcagttttt tcattctgtg cggagagcga ttgcgccgtg
      481 tgcgatacgt tttgcgcgct cgccgcgact tcttctacac cggaattcac ttcttctatc
      541 gaggccgccg tgttttgtag ttgtctgtcc gccgcgtgag attgcgcggc tacttcttca
      601 ctcaacgcaa gctcttcctc agaaacagca gcaaggttgc tggacgcgct tttgatctca
      661 ttagaagcgt gtagaatcga tacgactgaa tccctcagct ccctgcccat cgtatcgagc
      721 gcttgcgcca tctgcgcgat ctcgtcgctc cctttcgcct cgaaagaaac ggtcaagtct
      781 cccttcccga aggtatctac tcgcctggcc aactctttca acggcttcga tatgagggtt
      841 ccggtgagta gcgagatgag caccgtcgcc ccaacgatga tcgcaaaggc tacgagcacg
      901 atgatcaata gaaatatgct cttctctccg acttcacttt cggagataac cgctgtgagt
      961 ccccagttca ttgtgccgcc cacaggctcg tacaacgcga tttctttaga cccggatggc
     1021 ttcgtcattt tccgtaatcc cgatttcccc gataacagct cgcgaccaac ctcttctaat
     1081 cctttgtacc ccacttttgc gctttctaga agattcaagt ccatcacgaa cttcgcttcg
     1141 ggatgcgcga tgatgagtcc tgtcccatct gttaagaagg tatagccctc cttacccaac
     1201 cgaatcgagg agaccttctc gcttagcgcc ttcagggtaa ccgtcgcgcc gactgctcct
     1261 attacctttc ctttaggatc tttcgcttga acaacaacgg taaagactgg gttgttcgat
     1321 tgtttactga ctactgcgtt gctcacggca tatggtttcc ctttatggat cacttctcga
     1381 aagtagtccc tatcattgag atcatagatt tctcctccgt ctgtcatcgc ttttccagag
     1441 gggtcgacga aaaacacaag ttcaaatagc ccgcccgctt cctttttcag cgaaacgagt
     1501 aaaggagtgt agcgtcctcg atcaatagac tgaatctcct cggtcaaacc gagcgtgtgc
     1561 atagtgctct taaggccttc aagccactcc tcaacgatcc ggacggtcgc attcaacgtc
     1621 tcttccgcag aactctgcgc aagtgcgaag gtcacatttc tcgcaatcga gccaatgata
     1681 attgcggcaa cgacaagcag gacgactgat acggggatga acaacaacag cagttttgtt
     1741 ttgacacttc tgatcttcat ttcgcgccct cccctttttg aatggacttc gtaatttctg
     1801 atattggaat aatcttttta tcgcttattg ttttcgatca ttttacctat tgctacattg
     1861 ttatgatagc ctatcgttgt taaacagttt ccatgttctt ctagcatatc atatatcggt
     1921 tctatatcaa agaatattga tagatttttc gcttctagag tttgttcgtt gtcaataagc
     1981 ttgttcggat aaatcattac tccagcctag atcgtcgttt cttttcatcc atcgaagtcg
     2041 ggcaacttac tcaaatagcg ccttcatttc gatggatcgt gtcgcgattc gatgagatca
     2101 taaaggcttt tcgtatacct cttaccctct accgttcagc gttatcgact ccgatacttg
     2161 gtctgctcca gcagaactcg gccattctta gtaaaatcgc agatgccaca ggatgaagat
     2221 tcgatgagga taaatcagga aattgaaaga gtattagaaa tagttgaaga tacagctcaa
     2281 aaataacaac tagcgagagc gacacttcga ggggatgttc atcgaaagac gcaagagagc
     2341 gaatactccg aaagtcgcgg gcagaagcac gaaggatccc gctatcgcgt cctatcgaga
     2401 gattgcaaaa cctgagttga agagtttcgt ttaaaaagcc ggacggtaag gattgaagtg
     2461 aaatttcctg ataaaaagcg gcggccaagt gccaggagaa gagtgcaaac cttgtgggac
     2521 tatcattgta ataaactaag gaaatgtttt gggttggcga aaaagcaaaa gcgatattac
     2581 gaacgaacgc cctggcggga aacaaaggcg cagggcgcga tcagcgtgag aacggggtcg
     2641 acggagtttt accgtctcac gacttcgttc aagggtgagg gttatttcga aaaaatctca
     2701 ttccaagagc aatggcctta tcgtagtgtc ccatattcgg gtgatagcct cggaagcggc
     2761 ccccccgatt tgtacaacag gaactccttt cctcgtcgcc tcgacgacgc gctcatcaaa
     2821 agggatgagg ccgatgatgt ctgcgtttct tcgcttcagg ttttctttca caaactgcgt
     2881 gtacgcttcg ctgatatcgt atttattgat tatcacgctg tagtggacgt tgaagtgctt
     2941 ggccgtctcc atcatccgct ccagatcatg ccatccactt tttgatggct ccgtcacgat
     3001 gatgagcgcg cgggccccgg taatagaagc aatcgcagaa caaccgatac cgggcgcacc
     3061 atcgaccaaa accgaagccg ccgcttcctt cagcgccttt ccgtcagcga gttttctgac
     3121 ttccgtgacg agcttaccac tcgtttcttc tcccgggaat aactccccgt ctgcgaaagg
     3181 tatcccgata gcgcttccat cgcgtaacca accggattgc atcgcttcca tcgttatcgc
     3241 gtcgtgaggg caagcgaaaa cacaggcttt gcacccttca caagtaagcg agttgatatg
     3301 ggccagatcg ttttccatat gtatcgcgaa gaatcgacat accggttcgc atagcccgca
     3361 acccacgcaa cgagtcggct caatgatggc cttctttcca ccgaaatacc gttcttggtt
     3421 ttcgcatcga aaaccgagca acagatgaag gttcgcggca tccacatccg cgtccgcgat
     3481 cacgattcgg gttgtgggcg gcctttgggc gagataggcg aaagaggcgg cgaccgtcgt
     3541 ctttccggtt cctcctttgc cgctcataat ggctatatgt ttggtcacgc gaatcctctc
     3601 cctatggctt cgataatccg ttcaaagacc ggaaggtatt tcggtagctc tttgaccacc
     3661 agcttcccct ccgcgtagac ggatgcgatt ttcctatcga aaggcagcgt agcgatcacc
     3721 tctgcgtcca gatgtcttat cttctccacg tactcgcttg agccggcgtc aaaccggttg
     3781 atcacgactt tgaaaggaat cttcatttcc gatacgacgg cgatcgccgc gcgcaggtcg
     3841 tgcaaaccaa acggggtggg ctcggtcacc aataaagcgg agtcggaccc ggacaaggat
     3901 tcgaccaccg aacaggatgc tccgggtggc gcgtcgagta tccgaatctg cgcgtctttt
     3961 ggagccaggt ttttcaactg acggatcacg ggaacagcgg agggttctcc gatattcaga
     4021 atcccttctc cgaaggaaaa gccctcctgt gtttttccca aacgaatatt cccgatcgtt
     4081 ttaggcaact ctgtgatcgc tttttgcgga caaagacgcg cgcataggcc gcacccgtga
     4141 cagagcgggg ccaaaaccgc cgtctttttc ggggcgatcg tgatggcgga aaattgacac
     4201 tctttcatac aaagcccaca ccgatcgcac aaactctcgt cgatgtgggg gatgagtttt
     4261 tcgacgggtt gtgtgcgaat cggaatgaac tgcacaaaca gagaggcatt cggctcttcg
     4321 gcgtcgacat ccaacagctg ggtggccaca cccttttgat ggaaaagcca agccaagttg
     4381 gtagagaaca gcgttttgcc ggttccccct tttccggaca ccaccgtgag gctacgcatg
     4441 ggtggccaga tattgcgata gggcttcttg ggcagtgacg ccggtagcta acgcgaattt
     4501 aatgcctaag gtgtcgagag cggctttcgc gtttggcccc ggcgccgcgc cgagtacaac
     4561 ctgtacgcct ttctgcgcag cgaactgcgc gaaacgagat cccattccgt gttcctgctg
     4621 gtaggggtta gcataggatg tccattgctc ggtttgcgtg tcatagatca gaaaataggg
     4681 tgcccgagcc agtcggccac acaccgatgc ctgagccgtg tcgccctcgg ccgttaccaa
     4741 tactttcata ctgttccctc ctgaccttta ggggagacta ctctttcccc agtcgttcct
     4801 gaatgtaagc caaccgttgc gtcaagtact gtttttcttg ttctaatatc tctcgttcag
     4861 tctcgggcgt catcggaaca taatcgccct cgaacggttc gccccaaaac cggcggaaac
     4921 ctaatcctct tccgaacccg cgtctaaaaa agcgcgctcg atagcctctc ggaactccac
     4981 aaccgattcc tctgccccaa acagggcccg ttccgtctcc atatggcatc gtctcacccc
     5041 ctttcacgta gatagttttg cctgcaaacg tttttgaaat gtctcaatct gatacctcgt
     5101 gtgttctaag tcctttgaga tgcccatcaa catatctttt ccaccgttcg ttatttcgta
     5161 cacctttttg gctggtccaa tatcctctgt cttccaatag gagcgaatcc acccgctctc
     5221 ttcgaaaacc atcaaagtcc ggtataaatg acccatcgcg cctactccgg gcagatgtat
     5281 tcccaagtcg gtcagttgct tcgccaactc gtacccgtga cacggttttt cgtacagtaa
     5341 caacagaatg tatccttcaa acagtacccg gccccttcca aaccgccctt gacctccgcg
     5401 atgtccgccc cgcatttcta tcctccatat atatgcttaa cgcatatata ttaccacaat
     5461 actctctctt tgtcaagcgc cttcgtattt agacttttta gaaaaaacaa agagaaaaag
     5521 ctcccgctaa ggacgcctat ctcgcgaaag aggaagtttt tatcacggaa gaattttgtg
     5581 cgggaataat atcggggcgc aatagaaaag tggaaagaag cggaaggaaa aaagcctccg
     5641 acagagtcac cattagagct cgtttgaccc ttcaaggggg taaagaagcg tatttttcga
     5701 ccccgcgatt tgctctcgga acgcgcgaag tgaaaaaaac catgccgaac taaagtccgg
     5761 cgtttccagg acgctcccaa ggcattctca aggcattctc agggctttct ctagaatctt
     5821 ttctctgtgt tttctgtgtc ctccacgcga accctgtttt tccagacaag tacaaaaacg
     5881 tatcgcgcca tcgtgaaaag aaggagcgct tacgccgcga tgatcatgca tgggagcgca
     5941 cccagtgaag agataaaaaa cccatgccaa actaaagttt ggcgttcaca ggacgctccc
     6001 agggcgttcc caaggcattc cccgggcttt ctctaggatc ttttctctgt gttctccgcg
     6061 ccctctgtgc gaaccctgtt tttcaagaca cttacaaaac agtatcgcgc cgtcgcgaaa
     6121 agaaggagcg cttacgccgc gataacccac gaagttcgac gttgccaggg cggtcccaag
     6181 gcattccccg ggctttctct aggatctttt ctctgtgttc tccgcgccct ctgtgcgaac
     6241 cctgtttttc aagacactta caaaacagta tcgcgccgtc gcgaaaagaa ggggcgctta
     6301 cgccgcgata acccacgaag ttcggcgttt ccagggcgtt cccaaggcat tctcagggct
     6361 ttctctagaa tcttttctct gtgtcctccg tgcgaaccca gtttttcaag acaagtacaa
     6421 aagcgtatcg cgccgtcgcg aaaagaaggg gcgcttacgc cgcgatgatc atgcatggga
     6481 gcgcacccag tgaagagata aaaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnaaccc tgtttttcaa gacacttaca aaacagtatc gcgccgtcgc gaaaagaagg
     6661 ggcgcttacg ccgcgataac ccacgaagtt tggcgttccc agggcgttcc caaggcattc
     6721 tcagggcttt ctctaggatc ttttctctgt gttctccgcg ccctctgtgc gaaccctgtt
     6781 tttcaagaca cttacaaaac agtatcgcgc cgtcgcgaaa agaaggagcg cttacgccgc
     6841 gataacccac gaagttcgac gttgccaggg cgatcccaag gcattctcag ggctttctct
     6901 agaatctttt ctctgtgtcc tccgtgcgaa cccagttttt aagacgcgta caaaaacgta
     6961 tcgcgccgtc gcgaaaagaa gaggtgctta cgccgcgatg atcgtgcctt gacacacagg
     7021 tcattcaagg tttcttcggt agaaggtcgt cccgccgtaa aaaaatgaga ggagcttttt
     7081 ttgagccacg agctcctcta tcagagaaaa atcgactctt tttctctcta acaggagtct
     7141 tagagtgctt tctcgaacgg gatggacgga tgtgatcgcc aagatatcgt atacaaccgc
     7201 atcggttacg aaaacgcgtt cctcgttctc atcacctgtg atacactcga tctcgtgtgg
     7261 acaaacacct ctaacctgct tcacgatctg tccgagggtt tcggcatttg gccgctccac
     7321 cccgctttct gctggtggcc tcgtaggaac cattaagtag gatttcttag gccttatcga
     7381 ggacaaggcg cgggtgatat ccgcgatctc ggattcatta tcattcatcc ctttaatcaa
     7441 catcgactcg gtgacgagaa tcccgcgata tcgcctcgaa aaggttacga tcccttttaa
     7501 catctcctcg taattcaaag agccgtaggg gcggttgacc tttcgccaca tcgaatcatt
     7561 acccgcatcc accttcaaag agacccaatc ggcttccatc aacgcgtctc gaacttcttc
     7621 cattcccatc agcgaggagt tggagatcac cgccaccttc agtccgtgag ccttcgtgtt
     7681 ttctatctct ttttttaggc acagatccaa tgtgggctca ccgctcggca cgaaggtgac
     7741 ccaatccacg ggctcattcg tttctctgag ttgtcgaagt ctttgggtca gctctcgatc
     7801 gatctcctca ggagaaaaaa aggattcgcg cttcacctgc atcggataag ttcgaccgag
     7861 ctgacaatac acgcacgcgt agctacaggt cttggcggga atatgattga ttcccaagct
     7921 ctgacccaaa cgacgggaag tgactggacc gaaaattaac attcgcctac cgcctccctt
     7981 tcggatgtca tcccaatacg ttatcatagc acgaataggc cttcgaaaac tggcctatcc
     8041 tccggtttta agtttcccca ccgcgaaaac tattccgaac aaaaatcgac cgcgcatcct
     8101 tcaccgatat gtgctataat ccttattgtt tcatttcatc tataccaaat ggaggtgttt
     8161 gtatgaagtt gaaagggatg tttcttgtgg gtattctgct gatctgcgcc gtcttcgccg
     8221 ccaccatcga attgaccgtc tggacgcacg aagacccgaa caggacgctg ttggaagagc
     8281 ggtatatcga agaattccag aagctttacc cggaagtctc cgtcaaacgc gtcacctatc
     8341 cttcagcgaa gattaaggaa acagtattga ctgctttctc cgccggcaaa ggcccggata
     8401 ttttcaacat ggaagtgcaa gacgagtatc cctacatcgt gaaccgaagg gtggctccgg
     8461 tgaacccagc ggctttggga tacaaaaacg tggaagagat ttatgccgcc tatctcgaag
     8521 gcaccctcga tcccgtgtct tatcagggga aactgtatgg ggtacccttg gagatcacca
     8581 actggtgtat tttcgtgaac acgcggtaca ccaaggcgct tgggctggac tttgaaaaca
     8641 actatccgaa gacctgggaa gagttaatgg aagtatcgga caaactgacg cttcgaaacg
     8701 gagagatcat cactcgacgg ggtttcgact ttcggtatcc ttactatctc accattttca
     8761 ccccgatggt cgagcaaatg ggtggctacc tgctcaacaa agaaggaacg gaagccatta
     8821 tcaatcctca agcttgggtg gaagtactaa cctttatgaa ggaatggggc cccagtggac
     8881 gcaatcttgg ttctccgact tacaccgccg cgagaaagaa ctggaataaa gacgatgggt
     8941 caatcgttat gatcgagtcc ggactctacc aaatccttcg gctgaaagcc gaaaatccgg
     9001 agctctacga aagcaaggaa tggatggtca tcccctggcc cgtcttcgaa aacgccgtga
     9061 accacgtaaa aggaaattat tacgggcact actacgtggt caacgcgcaa tcctcccctg
     9121 aaaagcagga atgggcgtgg aaactgatcg cttatatgtt gaagcatacg ggcgaatact
     9181 tcgaagaagt cggtttaatc caaccgacgt acgaattgat ggagtcggag gttttcaaaa
     9241 acttccctta cgcgaacgtc tttatggagg atatgagcca agcggggtgc gtgttgctgc
     9301 accaggcggg gccacgcttg gaacaactca ttcgcgaagc agtggaatcg gtcatgctga
     9361 gcggagttac tccggaaaac gccgtgaaga cgcttaaggc gaaagcgacc gaggtactcg
     9421 aggactatta aaaactttac gtttcatatt cgaacgggaa gtcctttagc cctttaaaaa
     9481 gggcttctcg ttttttcaag ggggagttat gaaatctgga atagagaaga agaaagcgcg
     9541 ttggggatgg tttttcgttt tgcccgcgat tatctatttt tcgattttta gcttttttcc
     9601 gatcataaac gccttccgca ccagcttgta tcgttggaac ctcctttcgc tcaacccgcc
     9661 gcgcttcgtg tggtttgaaa actatatccg gctctttcaa tcggaggcct tctggaattc
     9721 ggtgaaggca acggccatct tcacggtggg caccttcagc tcgttattga tcggagggct
     9781 ggccttgtcg atcttgatct tttcacgaaa acgctttcga acgttttttc agatggcgtt
     9841 atactctccg gcagtcttat ccaccgtcgt tgccgcgacg atctggttat tgattctgga
     9901 tcccaggggg ttgggcaatt cggtgttgaa cgccgtacta gggacgagtg gtaaagacta
     9961 tcagtggttc gctaaccccg atatgtcgcg aatctcaacg atcatcatct acttctggaa
    10021 gtacatcggc cattttacga tactgttcat cgccggcttg tcatccatac cctacacgat
    10081 tcatgaggcc gcgatcatcg atggcgccgg accttggaaa aagtattggc ggatcacctt
    10141 cccgttgctg aagccgacgg tcgtcttagt ttgtgttctc tccctcatcc aatgtatgaa
    10201 atcgttcagc acgcaatacc tcttcgtaca atcgggcgcg ccgagggcgc cgatagacgt
    10261 cctgaccctc agcatctacg ataccgccat ccgcgaccat cagatcggca gggccagcgc
    10321 gatgagcatc atcctgtttc tcaccctcat cctattcagc tgggcgcaac tgagattgag
    10381 ccgaagcgaa gaagtgagtt tccaatgagc cgcgcggaca atcgtttaaa aaatacggcg
    10441 cggtctctgc gtgaaatcgt cttcgatctg atcttgctgg tcgtcctcgc catcgttttg
    10501 gtacccctgg tttttatgtt caccgcctcg tttatggaaa gcaaagaggt gctgacgatg
    10561 ccctaccgct ggatcccctc gtcttttcat tgggagaact acgttcaggc gatccaggga
    10621 aacgatatga aatactttta cgcccgcaac atcctcaatt cgctgatagt ggcgatcacg
    10681 gtcacgctga cgaattgcgt gttcgcggcg atgaccggtt tcggtttggc caagtaccgt
    10741 ttctttggaa aaagagccgt tttaatactg gttatggcca cgatgatgat cccttttgaa
    10801 acgattatga tccctctcta tatgctggtg aacaacttcg gctggcagga ctcttatatg
    10861 gggctgatta tgccattcct tgtcaacgcc tttggtatct ttatgatgcg ccaattcctg
    10921 ctgaccttcc ctgaagactt gttggacgcg gcgcgtatcg atgggttgaa tgagctatcg
    10981 attttcattc gtgtcgtgat tcccaattct ataccagcaa tgacggcttt gggaatcctg
    11041 accttcagaa gccaatggga cagtatgttg tggccgttgt tggtgacgca aagcgaagag
    11101 atgaaaacga tcccgttgta catcaccaaa ttcaccactg aaaaatacac gaacgagggc
    11161 gcgctgttgg ccgcggcggt catcgctagt atcccgctgt ttgtggtatt tttctctttg
    11221 gccaagtact ttatccaagg cagctccgtt ttttctggca cgaaagggta acgttcgtag
    11281 actgtgctat aattgaatat gacgcttacg gagatgatcg ccatgcatat agaggattgg
    11341 acaccctatt gtttcatcat ccgtgacctt ctctacgacg gctcttggga aacgatgaag
    11401 aaagacgccg aggggaatac ctttctcgag ccattgatcc aacaagcgca agatttggaa
    11461 aagctcgcgg gagagtcgct gcccggttcc ctctttctac cgacgggggc ggtagagatc
    11521 gctcatttct tttcacacca ccaatgggag atgtcccgac tggtcctctc aaagatggac
    11581 ggactctacg aactggcctc cgcagctttg gaagaagaaa attacgagga agcgcgcgcg
    11641 ttagccgaac tcttggaggg tataaaacaa gagaacgcct atagcgaaga gatatacggt
    11701 tctttggccg ttttaaaagg ggattacgcg caaggtatcc aacatctcga aatggcgatt
    11761 cggttggatc cgcgattcgt ccctgccctc agctctttaa gtcttgcata ttttaacact
    11821 caaaaatacg aaaaagcgat cgaaaccagc caacatatct tagaaaacag acccgaagac
    11881 ctcttggcgt acatcacgat cgtcgacagc tatatcaacc tcggattggc agaccaggct
    11941 ctagaaacgc ttcgacgtat gaatcaagcg atcccgagca atgtcacagg gaaaatccag
    12001 ctttatcgga tgctccgtga gcaccggcgt gacgcagagg ccgacgcggt tcggaaggat
    12061 attctgacga tctacccgtc tcagcctcac gagctggaaa tcttcgtcac gctcctcttc
    12121 gaaaccggca attttgaaaa ggccgagttg gagattaacg actacctcgc acgaaaccca
    12181 caccatactt acttaaagat cctactcatc gtccccttga taaaaaaagg gttcatacaa
    12241 gaagcccgac gtatcgtcga agagtttaag gagcagaagg tttggtacta ttttggaaaa
    12301 aaagaaacgc tcgggtcttt tatgagtgaa tccgaacaaa aaggatgcgg tcttatatga
    12361 tgctggcgat agcgggcggc agcggctctg gtaagacgac cgtcgccatt cggttatccg
    12421 agcgcttgaa agagcattgt gcgttattaa gcatggactc ctattacgtt gacctccctc
    12481 ccggcatcaa tccgggagat ttcaatttcg acgcgccgtc ggctttcgat ttcacccttc
    12541 tgaagaaaga cctaatggag ctgaaagcga gccgcccgat ccggatacct tcctacagct
    12601 tcattactta tcggagagaa ccgggtctgt tcacggagat gaagcccagc tctatcgtga
    12661 tcgttgaagg gatactggtc ttgtacgacc ctgagttacg aaagctcttc gacttctcca
    12721 tctacgttga aacccaagat gatgagcgat tgttgcggcg aatcgagagg gatatggtcg
    12781 aacggcagcg ctcgatcgag agcatcattc gccaataccg acgttttgtc gcccccgctt
    12841 atcgcagttt tgtggagcca cagaagcatt acgccgatat catcttgccg agcgggggat
    12901 acaacgaacc gggaatggag atcatctaca acgcgctcgt acaaaagatg cgccagagcc
    12961 gggaatagaa aaacgcggta tcgattgtgg ataccgcgtt ctttgtttcg ttgatctcgt
    13021 ctgtttcgat aatggattat tccactttca cttcgatcga gcgaggtttg cttttctctt
    13081 tcttcgggag ccgcaactcc aataggccgt tctcgtattt ggcggagatg tcttccggtt
    13141 ttgcgttctc tggtaaggtg aactgccttt taaaacagcc cgttcttctt tcgacgatgt
    13201 ggtagctctg gtctttctct tctttcgttt cctgccggac gctctctaca gagaggacgt
    13261 tttcttcgac cgaaatcttg atgtcttctt ttctaaggcc ggggagctca aattcaaaga
    13321 cgaattcctt ttcgttttca tagacgtcca cgctgggtat cttacaccct ttcggagcca
    13381 tcggacttcc gcctcttaaa aacgcgctga acacattgtc gatctctctc tgcatatcca
    13441 cgaaggtttt gtaaaaatcg tcttcccttg ctttccgaat cataatactc ataagtgacc
    13501 ctcctttttc attattttct tctacctata tagacgttag ttctctgatt ttatttcgtg
    13561 cttattatta gactttagtc gttctatatc aagtatcgtt atgtcttcag acgtaagaac
    13621 agtggtgttt attctcatcc cccgatttaa aaaaagaaaa ggcatcagag atgccttttc
    13681 tttcttcttt atacagagct tacttgaaca gttcaagcag attgttgttt tggaagatcg
    13741 aaactgcgat caaggacacc accgccatga tcttgatcag gatgtcgaga gaggggccga
    13801 cggtgtcttt cagcgggtcg cccaccgtgt ccccgatgat ggcggcggca tgcgcctccg
    13861 aatctttctt gatccccggg acgtttccgg attccaggta ctttttcccg ttatcccacg
    13921 ccccccccgc gttcgcggtg tagatggcga gcataatcgc cgataccgtg gcgcccaaca
    13981 gaaccccgcc aacgaattcc gggcctaaca aaaaccccga gacgatgggt gtggtgacgg
    14041 ctaacagcgc ggggaagacc atctcgcgta cggctccggc agaagatatc tggatacagg
    14101 ttttgtagtc cggacgcact tcgccggtga ggatacccgg ttgttcctta aactgtcgtc
    14161 ggacctcttg aaccatatgc cgcgccgctt tcgtaaccgc ttcgatcaga acgccggaaa
    14221 agaagtatgg tagcgcggct ccgataatga ttccggacaa ggtcaaggta ttgaccatat
    14281 tgaggatcag cgcgtaaccg ctcgtatccg taggctgcgc ttgcgaatac aagaaagagg
    14341 caaacagcga cagcgcggcg aaagcggcag agccaatggc aaaacctttt cctatggctg
    14401 ccgtggtatt ccctacagag tccagttggt ccgtgatcgc gcgcacttca tgctccaact
    14461 ccgacatctc acagataccg ccggcgttgt ccgcgatggg cccgtaagtg tccacggaca
    14521 ccgtggcgga cacgaaagaa agcatcccga tcgcagctaa agccaccccg tataaccctg
    14581 cgaagtaatc ggccgcaatg atagcaaggc cgaggacaat gactggcaag gcggtcgatt
    14641 tcatccctag cgccatacct tcggtgatcg ttaaagccgt cccttcaagc gatgatctgc
    14701 cgacacgctg agtaggtcgg tagtcgtaac tcgtatagta ttcggcaatt aatccgatcg
    14761 cgataccgct gacgattccc aagatcgcgg cataccaagc ggatagcgga ccgaaccgaa
    14821 agccggcggt ccacacggct tcatccttga agtagaagta cgaaaacaca ccgttgatca
    14881 caatcgtcga gccggcggag acccatgtgg agtagttgag ctccatacga ggatggtcgg
    14941 atactttctt gacaagcaga aacaggatac ccaggataca ggagatgatc ccgaaagagg
    15001 cgaacaatac cggataggtc atcagcttcg taaccaacgc ttcgggtaac ggtgaagccg
    15061 aggtcatcgt ggtatagtac atataggaag acagcaccac cccggaaatc aatgcgccga
    15121 cgaaactctc cagaagatcg gcaccaagcc ctgcaacgtc tccaacgtta tctccgacgt
    15181 tatccgcgat cgtagcgggg tttcttgggt cgtcttccgg tatgtgcgct tcggtcttcc
    15241 ccacgagatc ggcgcccata tcggccgctt tcgtgaagat accgcccccg actctgaaaa
    15301 acatcgcgat caccgagcat cccaacgcgt aaccggagac ggtcatcgtg aaggggataa
    15361 aactcacacc catccagttg gtgacaattc ttatttgccc gatatgcatc tgtcccatcc
    15421 actccccaaa gaccacatac acgatccaaa gccctaaaag cgcgaaccct ccgacacata
    15481 gccccatcac cgatccgccg cggaaggcca ctttcagcgt tttgcccaag ctcttggtgg
    15541 ttctggccgt gttcgacacg cgcacgttcg cgatgacggc gatcttcatc cctacccaag
    15601 cggcgctggc gctcatcagg gccccgatca ggaacgcaac gccggtgtac caagaaaccg
    15661 agagccaaag taaggcgacg atgacgatgg cgatcataga aataactttg tactcatgtc
    15721 ttaggaaggc atccgcccct tccttaatgg catcggcgat atccaccatc ttaggcagac
    15781 ctggatctag tttcttcact ccgaaaaagt tgacggcggc caaggctaaa gagccgacca
    15841 cggcgatcaa gatgattact gctgtcacaa gaaccctcct ccatagactg tatcattatc
    15901 ggacaaccac tttccgttga ataaaacatc gacgggccgc aaccgttcca actcggggaa
    15961 attgagagta aacgggtctt tgtcgaagag tagcgcgtcg gcacaaccgc ctttcctgat
    16021 gtttccaacg cgttcttcat cgaacccgct ctgagctgat cggaacgtat aaaattgaaa
    16081 aacctctcga atcgggaaaa atcggagcgc cgctcttatg ttgggccatg gatccactgt
    16141 ttcaacgggc gcatccgtgg atagagcaat tgggacccct tcttcgtaca gggctgtgaa
    16201 ggggtaaccg agcgtgcgat acctctcctc ccccaagact ttgggggcca agagcacgtc
    16261 ggaatcatag aaaatgggtt gcaaactgac ccataagttt tggcgtttaa tccggcggat
    16321 ttgatcgcga ctggccaact gaaggtggat aaggcggtga cgaagcgggt tatcggatgg
    16381 ggcgcgccgt tcgaagctcc ttagggcgat ttccagcgcc gcgtctccga tgacgtggac
    16441 acacagcgaa agcccgttct tttcgcaccc cttcacaaac cgatccaatt cttccggcgc
    16501 acagtagacg accccacgat agtcggtctc cccttcataa ggagcgttca aataggcggt
    16561 acgagccccg aacgagccgt ccaacaaaag cttcacagcg cggattcgta gatgatcccc
    16621 aaaatccccg aagtattctt gcggtctttc catccattca gaaaaatccg attctttttg
    16681 tggaagcatc ttttcgtaga tgcgcacccc ccggtgattg aggagcagag cgcgtaatgt
    16741 ccggtagtcg acgcggccta gatcgtcgct atggattgtg gtgactccac aagacaaaca
    16801 gcgaaccgaa gcatctcgca acgcctgggt taaagtcgtt tcggtgaaaa gggagcctgt
    16861 gggtatcgcc tcgagcgcgc gctctttcag aatcccgcga ccgatgttcg agccatccac
    16921 ctcgtcccaa cgctcaagcc ggaatgcctc tacggcctgg ctattggcac acgcgcagtg
    16981 cccgcattta cgagtcagca cgaccggccg ttcggaatcg atccggtcta tcacctgtcg
    17041 atcgggaaaa acgcctagta aaccttcatc ccaacctctg agccatacaa ccggatgttt
    17101 gacggagcga acgacctcgg ttaaggcgga gatcgacgac agccccgata aagaaggaaa
    17161 aacggagttt aaccctatcc ccaacaagtg ggcgtgcgaa tccacgaatc cgggcaacaa
    17221 aaagtgaccc gatagatctc tggaggggcc ggacaacggt tctcgctttt cgaataaccc
    17281 ttccccgggc acgtctatat ccgcgatctt tccgtcccgc acggttagat ttcccgcccg
    17341 atagtcctta accgaagagt cccaatatag ggcattgaac aagtacaccc gtttcccccc
    17401 ttacattcgc cgatttccac accagttttc gccgtggaaa agacgtgctc gcgcggaaag
    17461 ccctcctatc caaagctcaa taacgaggag agagttcttg agcttgcgtc tctcgagcaa
    17521 aaaacaggtc caggaatcgc tgtagatcct cctcttctac ttcatccacg tcttcgaagt
    17581 aatcgatgtt ttttcgctta tcctcagaga aggcttctgc cgatttccac tcgatcgctt
    17641 cctccgcttt ctgcgggtgg gctgactggg cctccgaaat catcttctga gctaattgaa
    17701 tgacttttga cacatctaat tcatcgaata tcgtatccgc ccctgccgcg gcgtctttca
    17761 ccgtttctcc tgccgttgcc tccgatttag cggcctgtaa gatctcattt ttcgcgatct
    17821 gggcgatctc ttcttcggtc aacgccacgt cacccgtcgc tcttttttct acgtctatct
    17881 ccgggttctc cgtgaagaaa cgggcgatat cctctatctc ggccgatgcc atcttctgga
    17941 tatcgatctt ctcttcaaat ggcgtatcgg tcagtaaaag caattcggcc tcgtcgttct
    18001 tggaggatac agatatctgt gagatctcgg gatgactggg actaacggtg acagggatcg
    18061 cgttcttggc cagtactaat cgaacggcca aacgactgaa aacggcgaac ttcaattcca
    18121 ttaacagcga gagggatagt gtcactgcga aaacaattcc gacgatcatc gggatcgaca
    18181 ggctcttttg acccgcgcta ttcgtctgac tcatagtgga taagggggtc gtttcgggta
    18241 ccggcgcagt gttttgaatc ggagtgaagt aataaaaacc ggtgcccatt tttccttcga
    18301 tatcgctaat cttattcaga agatcaaccg ctatcggatt gtctcggtaa aggctcaaat
    18361 tcactttcgc gatgctgtag ttctggttct tgaaggcgct gacgcccaac ataaatttcg
    18421 cgtttggggt cttcgcttcg aggtccggat ccaattggag cgctctggac aagtattgct
    18481 ccgcatcgac gtaattccct tggttatagg cgttctgccc tgcgaagaac aaggacatcg
    18541 actcgtcttt gtaactttga gggaaaagga cgacgttccc caaaagaatc gcgatgagga
    18601 cccatagaac gtgtttcacg gtatcaaccc gatctttcga aggccgaatt gcccattttc
    18661 ggaggttaaa acccacattt cggcgttacg gatctgcgcg tcaatgactt cgccatagtc
    18721 tttaagatcg aaatacgacc cttctgacgg ttttggtgat agagaaaggc cggccacctt
    18781 attccttccc accgcgtaga cggcattatc cgtcgagagt agtttgaatc cccctatttg
    18841 attggaagag gcttcattga cgatctttcc gtcaagactc agcgccacaa gacgataggc
    18901 ggaagtgaga ataaacaagc ggctttgttt cagaagaccg tctaaaaaat gttcgttggg
    18961 tttaatcggt ttgtagatct ccacgatttc ttttccatcg taaaggtaga gaccgatatc
    19021 ggtcaatata tagggataga cgccgtagag aatgatcttc cccgtcatac ccgggatgcg
    19081 gaaggaggag gtgggggtct ctaatatcaa cccgccattg tccaccaaat agtattggag
    19141 atcccccgtg aaggccatgg atctcacttt cccacggata cccggatagg ctttggtgag
    19201 tccgtgagtt ttcacgtaat ggtagaggat ttcttcgcta aaaaacgcca gctctccgtt
    19261 gtctagggcg aaggcttcga cctctccctt aaaaatgcgg gtcggcagac cgttcacaat
    19321 acggatcacg ccttcggcat cgaagatgaa atctcctaat ccgttggcca cgaaaccgta
    19381 gggagattgg acatcgggca aaggaatccg aaaactcgga taatgtacca gccgattgct
    19441 gaggtagaag ttttcgggca gggccaactg atggtagtcc tggtgaccgg gaacgtatcg
    19501 atgtaagccg gtggtcgtgc ctgtcgagga gggcgtagcg acgccgtttt ctttgaccgt
    19561 tgcggttttt gtcgttgtgg cgccttctat gaccggaagg gtcgaaacca gtcttacttc
    19621 ggtcggttgg ccggttgtct cttgctcaac ccgaactgcg ctcgtggcct caacggtcga
    19681 gggaatagag gattctgtcc gagtagcccg aggggagaag gcctctccaa cggatgaatc
    19741 cttatccata ctgctcttcc aggaatcaat atcgaccccg gctgttgaga caggtgtcgg
    19801 caaagaaacc gtcgtcgctt gcgcatacgt ctgagtgcca atcgtagaaa actggaagag
    19861 gcttaaagga cccgttaggg cctgtgtgcc ggtagacacc aattccggat actcgtagct
    19921 ccattctcgg tataggtcgt cctgatactt cggaacagcg acctcctcaa tgccttcttc
    19981 caaaaattgc ttgatgaata gcaaggtctc tttttgaaca tagttctcat agaaagaaaa
    20041 gtcgaatact ttcccgtata ggtttttcag gcctttgggg ttttctatcg tcggcaaaag
    20101 cgcgctgtag atcgaaacga ccccatcccc tttcgtagcc gtcagctcag ggtagaactt
    20161 cgcgagctga gacctctcga tatcgtcctg gaaacctggg gagctacccc ctataaaggc
    20221 gtattctatg tccgttctga tcgactgcgc gagttcttcg acgatcttgc tgtcagggag
    20281 catttccgct gcgtaggcat tcaccttttc gagatagctg aggttgttct tttcgatgaa
    20341 gcgaatggtg tcttcttcca aaccatagag tccggctaag acggcttttt ccttcccgta
    20401 aagaagattg aggaacgacg ggttgacgat attggtgcca ccgtttggcg tgctgattaa
    20461 gacgacctta cgcacgttgg tcacggggta gttttctatc gcataacgcg taatcaagcc
    20521 gcctaccccg tgcgcgatga tatccatcgg cttcccgtag ttctgcaagg cctgcgctaa
    20581 tttcgatgcc tcgaaggcga tgtaactggt gcgcagcgtt ttttcgaaga agttggtcgc
    20641 ttcgctcaca tagtccatcg accgggtttc caccaaggga tagctgtagg accagatcgt
    20701 ccgatcaggg aaggcaactt cccagatatt gttgccctgc gggttgacat tcgatatggt
    20761 attcggcaaa aaacccaaaa agttccggtc cacaccgggc acgatcagta ggtggggttt
    20821 tagcgtttct cgcttctcta tcttcttctg aagtccccat tcgaccttat ccgggttcga
    20881 tacccgtatc ccgattacgg agccgtgata ggaacgcgcc tcaaccacta tcgtgtcgtt
    20941 gattatcttc acttccgccc ctgaaaaggg ttgggcgatc gggttcttgg gatcgtcgat
    21001 atagtagagt tcgatattgt tgaaacccga gccgaaatag aattcggaag gaaagacata
    21061 ccgaaaaagc attggcacca acgcgagatc atctctaccg tccacgggta agagctcgta
    21121 taggtttccg gtaaaccggc ccagcgattt taacgcgtag agcgggtctc cggagcggat
    21181 cagtcgaatt gtgaaatctt tcgtataagg aaaggcgctg ggcgggatga cgatactgaa
    21241 atcggtgatt tcggcttttg cctcggactg aacggatgcc cgcgtagctt tggagactcc
    21301 tctttgtttt atagcgagta gcgcgaagat ggccaccaat acccccaacg cgacgaggat
    21361 caccaaagcc aacagacgat gagagagaag gaaatcgata ggctgtttga gcacgctcgg
    21421 cagcttgaat cctccgctaa ctcccttttc ttttgccttt ttcggctctt tcgaagcctt
    21481 aggaggtctt tctttaggtg ttttctttgg tttcttccgc ttctcttcgt tagccatcgg
    21541 tgttgctccc ctagttgcga aaattcgaat ccggtttctc ccaggtcggt tttattttct
    21601 cctgatcagt tctctgctct cgttcaaaaa acacacgagc gggtttgctt gcgaagaact
    21661 cgagtgtctt cctatttctt cagtcgcttc ccgccataaa cgatccgctt gctcttccgc
    21721 ctcttgaagt gttttcaagc ggagtatcgt ttgcttcccc gatttcagat ccttttgggg
    21781 tgttttaccc agttcctcga aatgacctgt ttcatcttta atgtcgtcat acatctgaaa
    21841 ggccaaacca atctttcccg cgctctcgtc taaggccgcc aaagccttgg ccggtatctg
    21901 ttttaagatc gcgggcgccg tgatggcgaa ccggatcaac ccccccgttt tgaggagata
    21961 catcgctgtc aactcttttt ctgacagcga tagtcccgtt ttttcgtaat agatgtcgta
    22021 agcctcgcct ttggctagat ccatagccac gcttaggaaa acgtcgatca gtagatcatt
    22081 tttcaacgga aggagggttt tcacggatag aaaaaacagc tcgtccgcgc atatcacggc
    22141 caacccttgg ttgtagcgct tatgggtcgc caggcgcccc cttcggtaat cgtcatcgtc
    22201 tatttcgggg aggtcgtcat ggacgaggct ggccgtgtgt aggatctccg ttgcagcccc
    22261 caggcgatag gcgtccgatt cgctcgctcc gaacgcttcc gcactataat aacataattg
    22321 gggacgaaat cgctttccac ccgaaaggaa ggggtagcgg atgatttctt cccaaaagg
//
LOCUS       DAOPXN010000013        21507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2435_length_21507_cov_5.285381, whole genome shotgun sequence.
ACCESSION   DAOPXN010000013 DAOPXN010000000
VERSION     DAOPXN010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 21507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21507
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2435_length_21507_cov_5.285381"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1033)
                     /locus_tag="PKM49_01450"
     CDS             complement(221..1033)
                     /locus_tag="PKM49_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HNY81512.1"
                     /translation="MSFKPAEVFESYCCTTSLAKTKMTPTKLLLLGILAGVFIGFGAI
                     FATTAMTGVTVLGLQKAIGGLVFCVGLILVVVAGAELFTGNVLLLIPLMEKKIDSSGF
                     LKNWIIVYFANFIGSLLLAWIFLGTDLWRAGTGLTPIGEQAMSIANAKSGLTWGQAFF
                     RGMLCNILVDLAVIMAMAAKSTAGKIMAIIFPISAFVASGFEHSIANMYFIPAGLFIK
                     GAAAADAVAKFSNLNWGNFLFNNLIPVTLGNIIGPLLFLCIPYYYVYRKKSA"
     gene            complement(1043..3760)
                     /gene="fdhF"
                     /locus_tag="PKM49_01455"
     CDS             complement(1043..3760)
                     /gene="fdhF"
                     /locus_tag="PKM49_01455"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNY81513.1"
                     /translation="MQINAFIDGKPYSFDRPMTVLEACKEAGREIPTICYHPSVKSIG
                     ACRICVVEVEGFRNLQAACTLPLSDGMKIKTQSPRITSSRKMNIELLLSRHPNVCMTC
                     ESNGNCKLQALAYEYGIEEPAYGVQLRGLEPDDSSLFIYRDLNKCIQCQLCVRVCDEV
                     ENMSIYSMAYRGYESLPEPPFGLPLGSSQCVSCGQCASICPVGAIVEKPSMGKARWWE
                     LKAVKTTCPYCGVGCQLDMYYHPKTNEIIKVMPSEENPEVNDGIATCVKGKFGFEFAN
                     HPDRLKKPLIRRDGKFVEVEWEEALDYTADRLKEMLRKYGPEGIGLLGSAKCTNEENY
                     ILQKFSRAVFKTNNVDHCARLCHASTVAGLAMSFGSGAMTNNLLDIQHADVILLTGSN
                     PPENHPVYGSRIKKTIRENGTKLIVADPRKTELVDYATIWINHKPGTDIALFSGLLHI
                     IAAENLADETFIAERTEKWEEMKESLKGFDPKTVSEITGVPVDLLYKAARLYAEAENA
                     AIIYAMGITQHACGTHNVISLANLAMATGNLGRPGVGVNPLRGQNNVQGACDVGALPN
                     VYSGYQRVGDPAMKKKFEEFWGVTGLPTNIGLPVTEMIGHPNIKAMFVMGENPAVSDP
                     DSEHVEKSLAALEFFADTDIFITESNVYADVILPAASTFEKDGTFTNTERRVQRIRKV
                     IEPIGESKPDWWIVQELSKRMGYEMNYQSAEDIMNEIRRCTPSYAGMAYHRMEKIGLQ
                     WPCRSDEDPGTSILHTQKFAHPDGKGKFTPMTYNDPPETPDAEYPFYLSTGRILYHYH
                     TGTMTRRVKAIHEKEPEVKIEINPTDASSLGVKTDDLVRLTSRRGSIEGKVLVTERSV
                     PGLVFVPFHFAEARANTLTGNFLDPIAKIPSFKVSAVKIEKIDHKTAEV"
     gene            4444..4956
                     /locus_tag="PKM49_01460"
     CDS             4444..4956
                     /locus_tag="PKM49_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNY81514.1"
                     /translation="MEVFSLSLEQCTVCARHETLYQELDAFIEKNRHRPGALIEVLHK
                     AQSLFGFLPAEVQAFIAKQLGIPESTVFGVVTFYHYFTMKPRGQNQVKVCLGTACYVK
                     GADRVLERLKEELKVGIEEVTQDGRFSIHEVRCLGACSMAPVVLIGEKDFFGRVTPDE
                     VPKILKKYRG"
     gene            4964..5341
                     /locus_tag="PKM49_01465"
     CDS             4964..5341
                     /locus_tag="PKM49_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNY81515.1"
                     /translation="MAKVKSLEELMKIKEKSLKDLQMRDTEKKGKIVVAMGTCGIAAG
                     AKDTLQAIVRELADNKVDDMSVVQSGCMGLCEVEPTVEVHMEGQAPIIYGHVTPEQAR
                     RIVQQHILSGKVVSDLMVKKGEI"
     gene            5342..7204
                     /gene="nuoF"
                     /locus_tag="PKM49_01470"
     CDS             5342..7204
                     /gene="nuoF"
                     /locus_tag="PKM49_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNY81516.1"
                     /translation="MSATDKTSIASEAPAPRVEARAATNTILLCAGGACISSGEESCL
                     QALRATLEKYALQDVVKVVETGCMGMCDAGPLMILYPEGVFYQKLKPSDMEQIVSEHL
                     LKGRIVESKLFVDPSTKVKITSAYDDHPFFTKQVKIATRNMGVIDPMSIEEYIARDGY
                     FALRKVLQEMTPEQVIDTLKKSGLRGRGGAGFPTGLKWELTRKVPGKEKFVLCNADEG
                     DPGAFMDRSILEGDPHSVLEAMAIAGYAVGAQKGYVYIRAEYPLAIERLTEGIKQAKE
                     YGLFGENIMGSGFSFDLEIRMGAGAFVCGEETALIESVEGKRGQPRVKPPFPAQKGVW
                     NKPTLNNNVETYANIPPIILNGPEWFTRIGTEKSKGTKVFCLAGKIKNTGLVEVPIGI
                     TLREVVYDIGGGIPNNKSFKAVQTGGPSGGCIPAEHLDMPIDYETLKTIGTMMGSGGM
                     VFMDEDTCMVDVAKFFLQFSVDESCGKCTPCREGNRVMYEILERITNGEGEEGDIERL
                     ETLGQSIIDSSLCGLGQTSPQPVLSTIRFFRDEYEAHIREKRCPAKVCRSLIRYHIDE
                     SKCVGCTACARVCPVAAISGSLRKPHRIDPEICTRCGSCIEVCRFGAISKITPI"
     gene            7298..7774
                     /locus_tag="PKM49_01475"
     CDS             7298..7774
                     /locus_tag="PKM49_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNY81517.1"
                     /translation="MREIIEAVEKEQSLEEGDILLNCLHRVQDVYQNFVPSEAAELVA
                     ERLKVPLSKVYEVLTFYTMFSTKLRGKYVIRVCNSLPCHVTGGSQIIDALFKELKVGF
                     GGTTEDGLFTLEKTGCLGLCGVGPVIMINDEFYGNLTVDRVAEILADYRARGEAGV"
     gene            7771..9405
                     /gene="nuoF"
                     /locus_tag="PKM49_01480"
     CDS             7771..9405
                     /gene="nuoF"
                     /locus_tag="PKM49_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNY81518.1"
                     /translation="MKAQTVLVSVDSGSILKGARYYKELFQELVHRYNLDSMVDVLET
                     GSLGIYREGVLVSIFPDGIYYEVRSKEDVERVVSEHLLKGRAVSALMIDEEIIKKDLP
                     KEDAMTVETRIVLRNVGVIDPLQIEEYIARDGYMALGKVLSAMKPKDVIDILKESGLK
                     GRGGAGFPTGRKWEFTAQTDSDVKYILCNADEGEPGTFKDRLIMEGDPHSVIEAMAIA
                     GYSVGASKGYIYIRGEYYGSIDNLNRAIQDAYEWGFLGQNILGTAFSFDLTIRLGAGA
                     YICGEETALIESIEGKPGRPRFKPPYPPVAGLYQKPTVVNNVETFANVPWIILNGPKA
                     FKKIGTASSSGTKVFTLVGNIRNRGLVEVPMGTTAYDLVYRYGGGLPGGKDLKMVQTG
                     GTAGTFVRPDQLNVKLDFDSGKLGVSIGSGVILAMDTDTCAVEAAKVAMEFFEHESCG
                     KCTPCREGTRLAVSILDSISKGKGKESDLDLLLSLADTMEETSFCGLGQAVPMPLRSI
                     IENFREEFLRHIRKEPCPVCVYEKPKKRKAGSIVTL"
     gene            9540..9911
                     /gene="rpsL"
                     /locus_tag="PKM49_01485"
     CDS             9540..9911
                     /gene="rpsL"
                     /locus_tag="PKM49_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNY81519.1"
                     /translation="MPTINQLVRKGRAKVITKSKAPALAGNPQKRGVCVRVTTMTPKK
                     PNSALRKIARIRLSHGTEITAYIPGEGHNLQEHSVVLIRGGRVRDLPGIRYKIIRGPL
                     DSAGVEARRQSRSKYGAKTPK"
     gene            9933..10400
                     /gene="rpsG"
                     /locus_tag="PKM49_01490"
     CDS             9933..10400
                     /gene="rpsG"
                     /locus_tag="PKM49_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNY81520.1"
                     /translation="MRRRRSEIRETIPDPLYNDLMVTRLINSIMWDGKKAVAQKVVYG
                     AFDVIREKTGKEPLEVFKKAMDNIQPVVEVKPRRVGGATYQVPVEVTERRKASLGIRW
                     LIKASRSKKGRPMKTRLSEEILAAYENTGNAVKKREDTHKMAEANRAFAHFKW"
     gene            10431..12500
                     /gene="fusA"
                     /locus_tag="PKM49_01495"
     CDS             10431..12500
                     /gene="fusA"
                     /locus_tag="PKM49_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNY81521.1"
                     /translation="MSPNAMMTKRVRNIGVMAHIDAGKTTTTERILYYTGKKHKIGNV
                     DEGTATMDWMEQEKERGITITSAATTCFWKEHRINIIDTPGHVDFTIEVERSLRVLDG
                     AVAVFDICSGVEPQSETVWRQADRYHVPRIAFFNKIDKIGADFDMSMESMKKRLGANP
                     VALQYPVGVEDAFKGICDILRRKMLRWIDLEGLQMEESPIPESMVEKVEELREELVTK
                     VGSYDDEIMELYLTEQEISVDQLKRAIREATISGKIVPVFCGSAFKNRGIQPLLDAIV
                     DYLPSPIDVPPTEAVRGQEPFELSADPEGRLGLLAFKTVADPFVGRLTYLRVYSGTLR
                     KGSYVLNVNKNKKERVSRLIQMHADNRLDIDESVAGDIVAVIGLKFTATGDTLTAEDD
                     PFLLEKIEFPEPVIQIAIEPDSKNDEPKLSKGLSSLMEEDPTLKVHVDKESAQTILSG
                     MGELHLEITVDRLKREYAANIRVGQPKVTYRESIRGQAHAEGKFIRQSGGRGQYGHVK
                     IAVEPLEDGLRFEFVNRIFGGTIPNEFIPAVKTGIEEALENGIIAGYPVVGVKVSLLD
                     GSYHEVDSSELAFKIAGSLAIKEALRKASPYLLEPIMKVEITTPEEYLGDIIGDINTR
                     RGRVDKFDSRGTARILEAKVPLGELFGYATILRSLSQGRATHVIQFHQYREVDARTLE
                     KIMRLRG"
     gene            12538..13734
                     /gene="tuf"
                     /locus_tag="PKM49_01500"
     CDS             12538..13734
                     /gene="tuf"
                     /locus_tag="PKM49_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNY81522.1"
                     /translation="MAKEKFTRNKPHLNIGTIGHIDHGKTTLTAAITKTLSMGGTADF
                     IPFDQIDKAPEEKARGITINVTHVEYQTENRHYAHIDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHVLLARQVNVPAIVVFLNKVDAVDDEELVDLVEMEVRDLLNK
                     YDFPGDDVPVVRGSALLALESNDINNKWNQKILELMKACDEHIPEPKRESDKPFLMPV
                     EDIFTITGRGTVVTGRITRGIIKTGEDIEIVGLRPTQKTVVTGVEMFRKILDAGIAGD
                     NVGCLLRGTKKDEVERGQVLAKPGSITPHNHFKANIYVLKQNEGGRHKPFSKGYRPQF
                     YMQTADITGEIVDLGEGVEMVMPGDNLVMEIKLIKPVAMEQGQRFAVREGGKTVGSGV
                     IAQVIA"
     gene            13798..14106
                     /gene="rpsJ"
                     /locus_tag="PKM49_01505"
     CDS             13798..14106
                     /gene="rpsJ"
                     /locus_tag="PKM49_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNY81523.1"
                     /translation="MASQRIRIRLKAYDHELLDQSAKKIVDIVKTTQAKVLGPVPLPT
                     ERSIYCVLRSPHKHKDSREHFEKRVHKRLIDILNPSPKTVEALMKVDLPAGVDVEIKS
                     "
     gene            14128..14769
                     /gene="rplC"
                     /locus_tag="PKM49_01510"
     CDS             14128..14769
                     /gene="rplC"
                     /locus_tag="PKM49_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNY81524.1"
                     /translation="MSGLLGKKIGMTRIYKGNESVPVTVIQAGPCFVCQKKVKAIDGY
                     DAIQIGFEEKKERSTIKPMKGHFKKANVRPMRYLREIRVDNPDDYQLGQELNLGLFEL
                     GQKVDVIGITKGRGTTGVMKRWNFSGGEASHGSKFHREAGSTGQHTYPGRTFPGKKMA
                     GRYGNERVTVHNLEIVRIDVENNLIALRGAVPGAPGGLIMLRKAVRVNLKKAK"
     gene            14795..15475
                     /gene="rplD"
                     /locus_tag="PKM49_01515"
     CDS             14795..15475
                     /gene="rplD"
                     /locus_tag="PKM49_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNY81525.1"
                     /translation="MADIKVLDLSGRQVGDLTLRDDIFNVTPNKDLLFRYIDMQLANR
                     RQGTACTKTRAEVAGTGRKPFPQKHTGRARQGTLRSPHHRHGGVAFGPKPRDWSKKLN
                     KKMKRDALKSALSVRFQEGNLIVLEDLTMKAPKTKELKELLDKFELTGLKTLFVLPYK
                     KVAYENVLKSGKNLPKTKVIIADNPGLPEGEVCIDGLNVFDIINHEKLILTKGTVAKI
                     EEVLGNVR"
     gene            15465..15764
                     /gene="rplW"
                     /locus_tag="PKM49_01520"
     CDS             15465..15764
                     /gene="rplW"
                     /locus_tag="PKM49_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNY81526.1"
                     /translation="MSVRLTDMDVLLRPIITEKTTFMMGDSKYTFEVHTDANKYMVKS
                     VIEKIYNVRVDKVNILNVKPKPKKRGVTRGKTRGWKKAVVKLVDGYSIPELQSLR"
     gene            15784..16617
                     /gene="rplB"
                     /locus_tag="PKM49_01525"
     CDS             15784..16617
                     /gene="rplB"
                     /locus_tag="PKM49_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNY81527.1"
                     /translation="MGLKRYKPTTPSRRFTVLPDFSELTKNTKPEKTLLEPLKSKAGR
                     NHHGRITTRHQGGGQKRQYRIIDFRRNKTGIPAKVKSIEYDPNRSARIALLVYKDGEK
                     RYILAPRDLKVGDELMSGPDAEIRLGNALPLQRIPVGTLVHAVEFVPGKGAQISRSAG
                     SSVQIMAKEGGFALLKMPSGELRKVREACYATVGTVGNEDNSNVVHGKAGRKRRLGIR
                     PTVRGMTMNPCDHPMGGGEGKAKGGKQPQSPWGTPAKGYKTRRGKRNSDKFIVRRRTK
                     K"
     gene            16636..16920
                     /gene="rpsS"
                     /locus_tag="PKM49_01530"
     CDS             16636..16920
                     /gene="rpsS"
                     /locus_tag="PKM49_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNY81528.1"
                     /translation="MSRSTKKGPFVHPKLLKKIRELNQAGRKEVIKSWSRASTIIPEM
                     VGHTVAVYNGIKHIPVYVSENMVGHKLGEFSYTRRFGGHADKKAKKGEVR"
     gene            16935..17348
                     /gene="rplV"
                     /locus_tag="PKM49_01535"
     CDS             16935..17348
                     /gene="rplV"
                     /locus_tag="PKM49_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNY81529.1"
                     /translation="MAHAKRSIFHAERKERERETPVIEAAATAKHLRLSPRKARSIVN
                     TIRGQKVDKAFTILELSPQKPARIVLKLLKSAVANAEHNFQLSVDHLIVSEAFVNDGP
                     RMKRVWPRGRGRADILQKRMSHITVVVQDQSKASE"
     gene            17371..18036
                     /gene="rpsC"
                     /locus_tag="PKM49_01540"
     CDS             17371..18036
                     /gene="rpsC"
                     /locus_tag="PKM49_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNY81530.1"
                     /translation="MGQKVHPRGFRLGVSKDWQSVWFGGKRYSELLIEDQAVRNHISK
                     RRYKLKLRNGQTVEASGDIDEVKIERPSLNKMIVTIATAKPGILIGKKGAEITEIRNE
                     IHDLTKREVIINIKEIKTPEVVAQLVAENVAALIEKRTTHKRAMKRVMKNAMMNKALG
                     IKVMVAGRLGGAEIARTEWYREGRIPLQTLRADIDYGFAQARTKTGIIGVKVWIYHGD
                     VTN"
     gene            18112..18534
                     /gene="rplP"
                     /locus_tag="PKM49_01545"
     CDS             18112..18534
                     /gene="rplP"
                     /locus_tag="PKM49_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNY81531.1"
                     /translation="MLMPKRVKYRKQQRGRMQGKAKGGATVEIGDWGLKALEPHWVTA
                     RQIEACRVAMVRTVKRSGKVVIRIFPDKPVSQRAADSRMGKGKGNVEKWVAVVKPGRI
                     LFEIEGVPDDLAKAALRKASAKLPILTKIVARHAIGGE"
     gene            18537..18737
                     /gene="rpmC"
                     /locus_tag="PKM49_01550"
     CDS             18537..18737
                     /gene="rpmC"
                     /locus_tag="PKM49_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNY81532.1"
                     /translation="MKAQEIRGLTDQELVQKMEALKKDLMNLRFQLALKQLDNTNKMK
                     SVKRDIARIKTILTERELASQK"
     gene            18744..18992
                     /gene="rpsQ"
                     /locus_tag="PKM49_01555"
                     /pseudo
     CDS             18744..18992
                     /gene="rpsQ"
                     /locus_tag="PKM49_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            19073..19441
                     /gene="rplN"
                     /locus_tag="PKM49_01560"
     CDS             19073..19441
                     /gene="rplN"
                     /locus_tag="PKM49_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNY81533.1"
                     /translation="MIQQESVLTVADNSGAKKILVIRVMGGSNRANGSIGDIVIGTVK
                     EALPHTDIKKGEVVRAVVVRTHKEIRRPEGTYIRFDDNAAVIVDKQNVPKGTRIFGPV
                     ARELREKGFMKIVSLAPEVL"
     gene            19447..19785
                     /gene="rplX"
                     /locus_tag="PKM49_01565"
     CDS             19447..19785
                     /gene="rplX"
                     /locus_tag="PKM49_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNY81534.1"
                     /translation="MRMKKYNFHIRKDDLVLVIAGRDRKKKGKVISVIPKDATVIVDK
                     VNTVKKHQKPKQQDKSGGIIDKNIPIAVSKVQLICPSCDKQTRVGWKALEDGTKARVC
                     KRCGEMIDRK"
     gene            20005..20538
                     /gene="rplE"
                     /locus_tag="PKM49_01570"
     CDS             20005..20538
                     /gene="rplE"
                     /locus_tag="PKM49_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNY81535.1"
                     /translation="MKERYRSVTVPELMKKFHYKNPLQVPKVMKVVVNMGVGEGTRNR
                     DLFVNHSEELMLITGQKPVITKAKRAISNFKIRKGMPVGIKVTLRGKNMENFLFKLFN
                     IVLPKVRDFRGLNPNSFDGKGNYSFGLSEQIIFPEIRAEKVKRIQGMDIIIVTSAKNN
                     EESKALLSSLGLPFKKG"
     gene            20554..20739
                     /locus_tag="PKM49_01575"
     CDS             20554..20739
                     /locus_tag="PKM49_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNY81536.1"
                     /translation="MAKKSMVARWSRPKKYKVREYSRCKICGRPHSVYRDFGICRCCL
                     RKMALEGKLPGVKKSSW"
     gene            20757..21161
                     /gene="rpsH"
                     /locus_tag="PKM49_01580"
     CDS             20757..21161
                     /gene="rpsH"
                     /locus_tag="PKM49_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNY81537.1"
                     /translation="MWSDPIADMLTRLRNANSVLKETVDIPASNLKKAICEVLKREGY
                     IHDYKYMEDGKQGIIRIILRYKGSRRKREKVLNGVVKVSKNGRRIYLSHDKILPVKGG
                     LGIAIITTSKGVITDKEARRLGVGGEVIAYVW"
     gene            21181..>21507
                     /gene="rplF"
                     /locus_tag="PKM49_01585"
     CDS             21181..>21507
                     /gene="rplF"
                     /locus_tag="PKM49_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNY81538.1"
                     /translation="MALDKEKLHVLSRLARKPITVPANVKVSLEGRALTVTGPHGTLS
                     QSLHPFVSVKVEGGKITVHPDLEMMKRPAESKNYRAMTGTFFALIRNMVLGVTEGFQK
                     ELHVVGV"
BASE COUNT         5623 a         5289 c         6243 g         4352 t
ORIGIN      
        1 ccccccgtca agatgatcag cggtattctg cccgcccctt gagtgtccac gatcccctca
       61 cctttcgaat agcgtcgacc gcgttcggag aacacgcatc ccgcggggaa ggactcccgc
      121 gctcagccgg ggcctccgac tgaaaaaaac cgccttggcc ttctcccgca gaacgggata
      181 ggagccaaag cggtttttta ttttcaaacc gtgttacaac ttaagcggat ttcttcctgt
      241 acacgtagta atagggaatg cacaggaaca ggaggggtcc gatgatgttc cctaaggtga
      301 cgggaatgag gttgttaaat aggaagttgc cccaattgag gttggaaaac ttcgctacgg
      361 cgtccgcggc ggctgcgcct ttgatgaaaa ggccggcggg tatgaagtac atgttggcga
      421 tcgagtgttc gaacccggag gcaacaaaag ccgagatcgg gaagatgatg gccataatct
      481 tgcccgcggt gctttttgcc gccatagcca taatgaccgc gaggtcaacc aggatattgc
      541 agagcatccc gcggaagaag gcctgtcccc aagttaaacc ggacttcgcg ttggcaatcg
      601 acatcgcttg ctctccaatg ggggtaagcc cggttccggc gcgccacaag tccgtgccca
      661 ggaaaatcca cgccagaagc agcgaaccga tgaagtttgc aaagtaaacg atgatccaat
      721 tcttgagaaa accagaagag tcgatcttct tttccatcag ggggatgagc aagagcacgt
      781 ttccggtgaa gagttcggcc ccggcaacaa caactaggat cagtccgacg caaaaaacga
      841 ggccgccgat cgctttctgg agcccgagga cggtgacccc cgtcatcgcc gtggtggcga
      901 agatcgctcc gaatccgatg aacacacccg ccaaaatccc caagagcaac aatttcgtcg
      961 gggtcatctt ggttttcgcc aaagatgtcg tacagcaata gctttcgaat acttccgctg
     1021 gtttgaagct cataatgcta agttacacct ccgctgtttt atggtctatt ttttctatct
     1081 tgactgcgct gaccttgaaa gaaggaatct tcgcgatggg gtccaggaaa ttaccggtga
     1141 gcgtgttcgc gcgcgcttcc gcaaaatgga aaggaacgaa aaccaagccc gggacggatc
     1201 gttcggtgac caggacctta ccttcgatgc tccccctgcg gctggtcaat cgtaccaagt
     1261 cgtcggtctt cacacccaag gacgaggcgt cggtcgggtt gatctcgatc ttcacttccg
     1321 gttccttctc gtggatcgct ttcacgcgcc gtgtcatcgt ccccgtgtgg taatggtaga
     1381 ggatgcgccc ggtagacaga tagaacggat actctgcgtc cggcgtttca ggaggatcgt
     1441 tataagtcat aggggtaaac ttgccctttc catccgggtg ggcaaatttt tgggtatgca
     1501 agatggaagt gccgggatcc tcatccgacc ggcagggcca ttgtaagccg attttttcca
     1561 tcctgtggta agccataccg gcgtaactcg gcgtgcatcg ccgaatttcg ttcatgatgt
     1621 cctcggcgct ttgatagttc atctcataac ccatgcgctt ggagagctct tggacgatcc
     1681 accagtcggg tttgctttcg ccgatgggtt cgatgacttt cctgatcctt tggacccttc
     1741 tttcggtgtt cgtgaaggta ccgtcctttt cgaaggtgct cgccgccggc aagatcacgt
     1801 cggcatagac gttggattca gtgatgaaga tatcggtatc ggcgaagaac tcaagcgcgg
     1861 ctaaggattt ttctacgtgt tcggagtcgg ggtcagagac tgctgggttt tctcccataa
     1921 cgaacatcgc tttgatgttg ggatggccga tcatctcggt aacaggcagg ccaatgttgg
     1981 tcggtaaacc ggtgacgccc cagaactctt cgaacttctt tttcatcgcc ggatcgccaa
     2041 ccctttggta gccggagtac acgttcggaa gggctccgac gtcgcacgcg ccctgaacgt
     2101 tattttggcc acggagcggg ttcactccca caccgggccg gcccaggttg ccggtcgcca
     2161 tcgcgaggtt tgccagcgag atgacgttat gggtgccgca ggcgtgctgc gtgatcccca
     2221 tcgcgtaaat gatcgccgcg ttttcggcct cggcgtagag ccgcgccgct ttgtacagaa
     2281 gatcgacggg gacgccggtg atctcggaaa cggtcttcgg gtcaaatcct ttcaggcttt
     2341 ccttcatctc ttcccatttt tccgttcgtt cggcgatgaa ggtctcgtcg gccaagttct
     2401 ccgccgcgat aatatggagt agtccgctga acaaggcgat gtccgtccct ggtttatggt
     2461 tgatccaaat cgtggcgtag tcgaccaact cggtctttct cgggtccgcc acgatcagct
     2521 tcgtcccatt ttcacggata gtctttttga ttcgggatcc gtagaccgga tggttctcgg
     2581 gcgggttgct tccggttaga agaataacgt ccgcgtgttg aatatccaac aggttgttgg
     2641 tcatcgcgcc gctgccaaag ctcatcgcca aaccggcgac ggtcgaggcg tggcacaacc
     2701 gggcgcagtg gtcgacgttg ttggttttga acacggcgcg gctgaatttt tggaggatat
     2761 agttctcttc attcgtacat ttcgccgaac ctagcaaccc tatcccttct gggccgtact
     2821 tgcgcaacat ctctttcaag cggtcggccg tgtagtccaa agcctcttcc cactcgacct
     2881 ccacgaactt gccgtccctt cggatgaggg gcttctttag ccggtcgggg tgattggcga
     2941 attcaaaccc aaacttgccc ttcacacaag tggcgattcc gtcgttcacc tcggggttct
     3001 cttcggacgg catcactttg atgatctcgt tggttttcgg gtggtagtac atgtcgagct
     3061 ggcacccgac gccgcaatag gggcaggtcg tcttgaccgc tttcaactcc caccaacgcg
     3121 ctttgcccat actgggtttt tccacgatcg ccccaaccgg gcaaatcgac gcgcattgcc
     3181 cgcacgacac gcattgggag ctccccaggg ggaggccgaa tggaggttcc ggaaggcttt
     3241 cgtatccgcg ataggccatc gagtatatgg acatattttc cacctcatcg caaacgcgga
     3301 cacagagctg gcactgaata catttgttca ggtctcggta gatgaacagg ctcgaatcgt
     3361 ccggttcgag cccgcgcaac tgcacaccgt aggcgggttc ttctatgccg tactcataag
     3421 ccagggcttg aagtttgcaa ttgccgttcg attcacaggt catacacacg ttggggtgtc
     3481 gagagaggag tagttcgatg ttcatcttcc gcgaagacgt gatgcgcggg gattgggtct
     3541 tgatcttcat gccatcagac agaggaagcg tacacgcggc ttgaaggttc cgaaaaccct
     3601 ccacttccac gacgcagata cgacaagcgc cgatgctttt taccgaaggg tggtaacaaa
     3661 tcgtcgggat ctcccttccg gcctccttgc aagcttccaa caccgtcatc ggccggtcga
     3721 aagaatacgg cttcccgtct atgaaagcat tgatctgcat accttattac ctcctgaatt
     3781 cgtatattta gtggtctgct ggaattatat cataaagaca ggtcatctga ttttctggtg
     3841 tgtatatcaa gttatggggt gtactcaaga aacaaagaca gaatctatca catagttttt
     3901 agcccacgta gtcatcgcgg cgtaaacgcc acttcttttc gcgacggtgc gatacgattg
     3961 ggaacgccaa cttttaagtt ggcatgtctt gaaaaaaagc tcacacgtag ggcaaggagg
     4021 tcacaaagaa gagatgtgtt ttcgtgagtc aacgccaggt ttatgttttc tctgtgttct
     4081 ctgcgttctc tgtgcgaaac catcttttac ttttctaaaa atatgccgaa ctaaagttcg
     4141 gcgttcccag gcgtttctaa gcgctcccag ggcgtttctc tgtgcattct gtgtactctg
     4201 tgcgcaactt cttcttttct ttccttttga aaggaaggga aaaacatgcc gaactaaagt
     4261 tcggcgttcc cagaagacat gccgaactga agttcggcgt tcccaggcgt tcccaagcgc
     4321 ccccagaggg gcccgcgcct ttgacaatcc gcttttccgc tccttttttc cgttgatttc
     4381 cggttcggtt atgttataat tgccttcgga aaagtaagga agatcatcgg tatatggatc
     4441 actatggagg tgttttcttt gtccctagag cagtgtacgg tatgcgcccg gcatgagacc
     4501 ctctatcagg agctggacgc gttcattgag aagaatcgtc accggcccgg agccttgata
     4561 gaggttttgc ataaagcgca gagtcttttt ggctttctcc cggctgaagt gcaggcgttc
     4621 atagccaaac agttgggaat tcccgaaagc acggttttcg gcgtcgttac cttctatcat
     4681 tatttcacga tgaaacccag aggacagaat caggttaagg tgtgtttggg aaccgcttgc
     4741 tatgtgaaag gcgcggatag ggtcttggag cgccttaaag aggagctaaa ggtcggtatc
     4801 gaggaagtga cgcaagacgg gcggttttcg attcacgagg tgaggtgttt gggcgcctgc
     4861 agtatggctc ccgtagttct tatcggagag aaagatttct tcggccgcgt gacgccggat
     4921 gaagtaccga agattttgaa aaaatatcgg gggtgatcgg actatggcaa aggtaaaaag
     4981 cctagaagaa ttaatgaaga ttaaggaaaa atcgctcaaa gatctacaga tgcgggatac
     5041 cgagaagaag ggtaagatcg tcgtcgctat gggcacgtgc ggtatcgcgg cgggcgccaa
     5101 ggataccttg caagcgatcg ttagggagct ggccgataac aaagtcgacg atatgtcggt
     5161 cgtgcagtcg ggctgtatgg gtctgtgcga agtcgagccg acagtcgaag tgcatatgga
     5221 ggggcaggcg ccgatcatct acgggcacgt gactccggag caggccagaa gaatcgtcca
     5281 gcagcatatc ttaagtggta aggtcgtcag cgacctgatg gtaaaaaaag gagaaatata
     5341 gatgagcgct accgataaga cttcgatcgc tagcgaagcg cctgccccgc gggtggaggc
     5401 gagagcggcg acgaacacga ttctgttgtg cgccggcgga gcgtgtatct cctccggtga
     5461 agagagttgc ctgcaagctc tgagggcgac gctggaaaag tacgccctgc aagacgtcgt
     5521 gaaagtggtc gagacgggtt gtatggggat gtgcgacgcg gggcctttga tgatcctcta
     5581 cccggaggga gtcttctacc agaaactgaa accgtccgat atggagcaga tcgtttccga
     5641 gcatttgctg aagggtcgga tcgtggaaag caagttgttc gtggatccga gcacgaaggt
     5701 gaagatcacg agcgcttacg acgatcaccc gttttttacg aaacaggtga agatcgccac
     5761 gcgaaatatg ggcgtgatcg acccgatgag tattgaggag tatattgccc gcgacggtta
     5821 cttcgccttg cgcaaggttt tgcaggaaat gacgccggag caggtgatcg acacgctcaa
     5881 gaagagcggc ctgcgcggtc gcgggggcgc cggttttccc accggactga agtgggaatt
     5941 gacccgtaag gtccctggga aggaaaagtt cgttctgtgc aatgcggacg agggggaccc
     6001 cggggctttt atggatcgtt ccatcttgga aggcgatccc catagcgtgc tggaggcgat
     6061 ggccatcgcc ggttacgctg tcggcgcgca gaaggggtat gtctacatcc gagccgagta
     6121 ccctctggcg atcgaacgct tgaccgaagg gatcaaacag gcgaaagaat acgggttgtt
     6181 cggtgagaac attatgggca gcgggttctc tttcgatctc gagatacgga tgggcgcggg
     6241 ggccttcgtt tgcggcgagg agaccgcgtt gatcgagagc gtcgaaggga agcgcgggca
     6301 gccccgcgtc aaaccgccgt tcccggctca gaaaggcgtc tggaataagc cgacgttgaa
     6361 caacaacgtc gagacctacg ccaacatccc accgatcatc ctgaatggac cggaatggtt
     6421 cacccgtatc ggaaccgaaa aaagcaaggg cacgaaggtc ttctgtttgg ccggaaagat
     6481 aaagaacacc ggattggtgg aagtcccgat cggaatcacg ctgagagagg tcgtctacga
     6541 tatcggcggc ggcataccga acaacaagag cttcaaggcc gtccagaccg gcggtcccag
     6601 cggcggatgc attccggccg aacacctcga tatgcccatc gactacgaga ccttgaaaac
     6661 catcggtacg atgatgggtt cgggcggtat ggtcttcatg gatgaagaca cgtgtatggt
     6721 ggacgtcgca aagttctttt tgcagttctc tgtcgacgag tcgtgcggaa aatgcacacc
     6781 ctgccgagag ggaaaccggg tgatgtacga aatcctggaa agaatcacga atggggaagg
     6841 cgaggaaggc gatatagagc gattggaaac cctggggcag tcgatcattg actcttcgct
     6901 gtgcggtctc gggcaaacct ctccgcagcc ggttctctcg accatccgct tcttcaggga
     6961 tgaatacgaa gcgcatataa gagaaaagag atgtcccgcg aaggtgtgtc ggtcccttat
     7021 ccgctaccat atcgacgaat ccaaatgcgt cgggtgcact gcctgcgcga gggtctgtcc
     7081 cgtcgccgcc atcagcggct cgctcaggaa accgcaccgg atcgaccccg agatctgcac
     7141 gagatgcgga agctgtatcg aggtgtgccg gttcggcgcg atcagtaaga tcaccccgat
     7201 atagggcgcg ccgttctacg ggcgaaagag cagctaaaga acacaaaaac agatttcagg
     7261 gaggtgtacc gtggttgtgg atcagacctt ggaaaaagtg agagagatta tcgaagcggt
     7321 cgaaaaggaa cagagcctgg aagagggcga tatccttctc aactgcttac atagagtgca
     7381 ggacgtgtac caaaactttg ttccctcaga ggcggcggag ctcgtcgccg aacggctgaa
     7441 ggtgccgctc tcgaaagtct acgaggtgct caccttctac acgatgttct ctacgaagtt
     7501 gcgcggaaaa tacgtcatcc gcgtctgcaa cagcttgcct tgccacgtca ccggaggctc
     7561 gcagatcatc gacgccctat tcaaagaact gaaagtcggt ttcggaggga ccacggagga
     7621 cggtctgttc actttggaaa aaaccggctg cctcggtttg tgcggcgtcg ggccggtgat
     7681 tatgatcaac gacgagttct atggtaacct gaccgtagac cgtgtcgccg aaatcttggc
     7741 cgactatcgc gccagagggg aggcgggagt atgaaagctc aaaccgtctt ggtctccgtg
     7801 gactccggca gcattttgaa aggagcccgt tattacaagg aactctttca ggagctggtc
     7861 caccggtaca acctggattc gatggtggac gtgctagaga ccggctcgct tggcatctat
     7921 cgagagggcg tgctcgtgtc gatctttccc gacggtatct attatgaggt tcgctccaaa
     7981 gaagacgtgg aaagggtggt ttccgagcac cttttgaaag gcagggccgt ttctgccctg
     8041 atgatcgacg aagagatcat caagaaggac ctcccgaaag aagacgcgat gacggtcgaa
     8101 acccgcatcg tattgcggaa cgtcggggtc atcgatccgc tccagatcga ggagtatatc
     8161 gcccgagacg ggtatatggc actcggaaag gttctgagcg cgatgaaacc gaaggatgtt
     8221 atcgatatcc tcaaggagag cggcctcaaa ggccgtggag gcgcgggatt tcccacggga
     8281 agaaagtggg agttcacagc ccagaccgac tccgatgtga agtatatcct gtgtaacgct
     8341 gacgagggag aaccaggcac cttcaaggac cggttgatta tggaagggga cccgcactcg
     8401 gtgatcgaag cgatggcgat cgccggttac agcgtggggg cctcaaaagg gtatatctac
     8461 atccggggcg agtactacgg ctctatcgat aacctgaacc gagccataca ggatgcctac
     8521 gagtggggat ttctgggaca gaacatcctc ggaactgcct tcagtttcga tctgaccatt
     8581 cgtttgggcg ccggagccta catctgcggc gaagaaaccg cgctcatcga atcgatcgaa
     8641 ggcaaacccg gccgtccgag gttcaaaccg ccctaccccc ccgtcgcggg actgtatcag
     8701 aaaccgaccg tggtcaacaa cgtcgagacg ttcgcgaacg tgccatggat tatcttgaac
     8761 ggcccgaagg ccttcaagaa gattgggacc gcgtcctctt cgggaacgaa ggtcttcacc
     8821 ttggttggca acatacgaaa tcgggggctc gtggaggtcc cgatgggaac caccgcttac
     8881 gatctcgtct accgttacgg cggaggctta cccggtggca aagacctgaa gatggtgcaa
     8941 accggcggaa cggccggcac gttcgtccgc ccggatcagc tgaacgtcaa attggacttc
     9001 gattcgggga agctcggcgt ctcgatcggt tccggggtca tcctggcgat ggatacggac
     9061 acctgcgccg tggaagccgc gaaagtggcg atggagttct tcgagcacga gtcgtgcgga
     9121 aagtgcaccc cctgccgcga gggaacccga ttggccgtgt cgattttgga ttcgatctct
     9181 aagggtaaag ggaaagaaag tgatttggac ctcctgctga gcctcgcgga cacgatggaa
     9241 gaaacgtcgt tctgcggttt ggggcaggcg gtgccgatgc ccttgcgatc gattatcgaa
     9301 aacttccgcg aagaattttt aaggcacatc cgaaaagaac cgtgcccggt ctgcgtctat
     9361 gaaaagccaa aaaagcggaa ggccggatca atagtaactt tataatggcg aataggtaaa
     9421 ttttaattag agtaagcggg tgcggttgac aacgcgcccg ctttaattgt acaatacgcc
     9481 gtatttatta aagaagccga aaggagggct ggaaggagaa ggtttccttc atctttttaa
     9541 tgccaacaat caaccaatta gtgaggaaag ggagagcgaa ggtcatcacc aagtcgaagg
     9601 ctcccgcgct ggccgggaac ccgcagaagc gcggtgtgtg cgtgcgtgtg accacgatga
     9661 ctccgaagaa acccaactcc gcattgcgaa agatcgcgag aatccgactg tctcacggaa
     9721 cggagattac cgcttatata ccgggcgagg gacacaattt gcaggagcac tccgtcgtgt
     9781 tgatccgcgg gggccgtgtt agagacctac ctgggattcg ttataaaatc atcaggggac
     9841 cgcttgactc ggcaggggta gaagccagga gacagtccag atccaagtac ggagcgaaaa
     9901 cccctaagtg atggggtaaa ggggagtatg gaatgagaag aagaagatcc gagatacgag
     9961 aaacgatacc tgacccgttg tacaatgacc tgatggtcac ccgtctgatc aattctatta
    10021 tgtgggacgg gaagaaagcg gtcgcgcaaa aagtcgtcta cggcgccttc gacgtgattc
    10081 gtgaaaaaac ggggaaggaa ccgctcgaag tctttaaaaa ggcgatggat aacatacaac
    10141 cggtcgtcga ggtaaaaccg cgacgcgtcg gcggcgcgac atatcaagtt ccggtcgagg
    10201 tgaccgagcg ccgaaaggcc tctctgggca ttcggtggtt gatcaaggcc tcgcgttcta
    10261 aaaaaggtcg cccgatgaaa acgcgccttt cggaagagat cttggccgct tacgagaaca
    10321 ccggcaacgc cgtgaagaaa cgcgaagaca cacataaaat ggccgaggcg aacagagcat
    10381 ttgcgcactt caaatggtaa tgactccgcg agaatgacgg agggtctgtg atgtctccta
    10441 atgctatgat gaccaaaagg gtaaggaata tcggcgttat ggctcatatc gacgccggta
    10501 aaacgacaac gacggagcgc atcctctatt acaccgggaa gaagcacaag attggtaacg
    10561 tggacgaagg cacggccacg atggactgga tggagcagga aaaagagcgg ggtatcacga
    10621 ttacctccgc cgccactacc tgtttttgga aagaacatcg catcaatatt atcgatacac
    10681 ccgggcacgt tgactttacg atcgaggtgg aacgctccct ccgagtgttg gatggcgccg
    10741 tcgcggtttt cgatatctgc tccggtgtcg aaccccaatc ggagaccgta tggagacaag
    10801 cggatagata ccacgtcccg cggatcgcgt tttttaataa gatcgataag atcggcgcgg
    10861 acttcgatat gtcgatggag agtatgaaaa agcggttggg cgcgaacccc gtcgcgttgc
    10921 agtatcctgt gggcgtcgaa gacgccttca aagggatctg cgatatcctc cgcagaaaga
    10981 tgcttcggtg gatagacctc gaaggactcc aaatggaaga gtctccgatc ccagaatcga
    11041 tggtggagaa ggtggaggag cttcgagagg aattggtcac caaagtcggg tcctatgacg
    11101 acgagatcat ggagctgtac ctcaccgagc aagaaatatc ggtagatcaa ttgaagagag
    11161 cgatacgcga ggcgacgatc tccggaaaga tcgtaccggt cttttgcggg agcgctttca
    11221 agaatagggg gatccagcct ttactcgacg cgatcgtcga ctatctgccc tctccgatag
    11281 acgtgcctcc caccgaagcc gtcaggggcc aggagccttt cgagctttcg gctgatcctg
    11341 aggggaggct gggattgctc gcctttaaaa cggtggccga tccgttcgtc gggcgtctga
    11401 cctatttgag agtctattcc gggaccttga ggaaaggctc gtacgtgctg aacgtcaaca
    11461 aaaacaaaaa ggaaagggta agccgactga tccagatgca cgcggacaac cgcttggaca
    11521 tcgacgaaag cgtcgctggg gatatcgtcg ccgtgatagg cctgaagttt acggccactg
    11581 gcgatacgtt gacagcggaa gacgacccct tcctgctaga gaagatcgaa ttcccggagc
    11641 cggtgataca gattgcgatc gagcccgact ccaaaaacga cgaacccaaa ctgtcgaaag
    11701 ggctgtcctc cctgatggag gaagacccga ccttgaaagt tcacgtggat aaagagagcg
    11761 cgcaaaccat tttgagcggt atgggagagc tgcatctgga gatcaccgtc gaccggttga
    11821 agcgcgagta cgccgcgaat atccgagtcg gtcaaccgaa ggtgacctat cgcgaatcca
    11881 tacgtggtca ggcgcacgcc gaaggtaagt tcatccgtca gtccggcggg cgcggccaat
    11941 acgggcacgt gaaaatcgcc gtcgaacctt tggaagacgg gctcaggttt gagtttgtca
    12001 accgtatctt cggcgggacg attccgaacg agttcatacc ggcggtcaaa accggtatcg
    12061 aggaagcgct ggagaacgga atcatcgccg ggtacccggt ggtcggcgtc aaagtcagcc
    12121 tactggacgg ttcctaccat gaggtggaca gcagcgaact ggccttcaag atcgcggggt
    12181 ccctggcgat caaggaagcg ctgaggaaag cctcgcccta tttattggag ccgattatga
    12241 aagtggagat caccaccccg gaggagtatt tgggggacat catcggggat atcaataccc
    12301 gtcgggggcg cgtggacaag ttcgacagtc ggggaacggc gcgtatcctg gaagcgaagg
    12361 tacccttggg cgaactgttc ggctacgcga cgattttacg gtccctcagt caggggcgcg
    12421 cgactcacgt gatccaattc catcaatacc gagaggtgga cgctaggacg ttggaaaaaa
    12481 taatgagatt aagaggataa acaagcacaa tcatttaacc gaggaggtaa gattcgaatg
    12541 gcaaaagaga agtttaccag gaataaacca catctaaaca ttggaacgat cggccatatc
    12601 gaccatggga aaacgacctt gacggccgcg atcaccaaga cgctcagcat gggaggcacc
    12661 gccgatttta tcccgtttga tcaaatcgac aaagcccccg aagagaaggc caggggcatc
    12721 acgatcaacg tgacccacgt ggagtatcaa acggaaaatc ggcattacgc ccacatcgac
    12781 tgcccgggtc acgccgacta tatcaaaaat atgatcaccg gcgcggccca gatggacggc
    12841 gcgatcctcg tcgttgctgc gacagacggt ccgatgcccc aaacccgcga gcacgtgttg
    12901 ttggccagac aggtcaacgt cccggccatc gtggtgttct taaataaagt ggacgccgtc
    12961 gacgacgagg agctcgtgga tctcgtggag atggaagtgc gagacctcct gaacaaatac
    13021 gatttccccg gagacgatgt cccggttgtg agaggttccg ctttgttggc tttggaatcg
    13081 aacgacatca acaataaatg gaaccagaag atccttgaat tgatgaaagc ctgcgacgaa
    13141 cacatccccg aacccaagag agaaagcgac aagcccttct tgatgccggt cgaagacatc
    13201 ttcacgatca cgggtagagg aaccgtcgtg accggaagaa ttacgcgcgg catcatcaag
    13261 accggcgaag atatcgagat cgtcggcctg cgccccacgc agaagaccgt cgtgaccggt
    13321 gtcgagatgt tccgcaagat tttggacgcc gggatcgccg gagacaacgt gggctgcttg
    13381 ctccgtggca ccaaaaagga tgaggtggag cgaggtcaag tcttggccaa acccggatcc
    13441 atcacccctc acaaccattt caaagcgaac atttacgtgt tgaaacaaaa cgaaggcggc
    13501 cgacacaaac ccttctccaa aggctaccgg cctcagttct atatgcagac ggccgacatc
    13561 accggtgaga tcgtcgactt gggcgaaggc gtcgaaatgg ttatgccagg cgacaacctc
    13621 gttatggaaa tcaaattgat caaaccggta gcgatggaac aagggcagcg ttttgccgtc
    13681 cgcgaaggcg gaaaaacggt cggttccgga gttatcgcgc aagtaatcgc atagcacacc
    13741 attctacgaa ggagagccgc gctctccttc gttcaggaaa gaggagggaa gcaatagatg
    13801 gcatcgcagc ggatcagaat ccggttaaag gcttatgatc acgagctatt ggaccaatcg
    13861 gcaaaaaaga tcgtagatat cgtcaagacg acacaggcga aggttttagg tccggttccg
    13921 ttaccgactg aaagatcgat ttactgcgta ttacgatcgc cgcataaaca taaagattca
    13981 agagaacatt tcgaaaaaag agtacacaaa aggctcatag acattttaaa ccccagtccg
    14041 aaaacggtgg aagctctcat gaaagttgac cttcccgcag gggtcgatgt cgagattaag
    14101 tcttagtgaa ccgatggagg tgctcaaatg agcgggttgt tagggaaaaa gatcgggatg
    14161 accaggattt acaaagggaa cgagtcggtt ccggtgacgg tcattcaggc tggaccgtgt
    14221 tttgtgtgtc agaaaaaggt gaaggcgatc gacgggtacg acgcgataca gatcgggttt
    14281 gaggagaaga aagaaagaag cacgatcaaa ccgatgaaag ggcacttcaa aaaggcgaac
    14341 gtgaggccta tgcggtacct gagagagatc agggtggaca atcctgacga ttatcaactc
    14401 gggcaagagt tgaacctcgg cctattcgaa ctcgggcaga aggtcgacgt catcgggatt
    14461 accaaaggtc gagggacgac gggagtgatg aagagatgga acttcagcgg gggagaagct
    14521 tcccacgggt cgaagtttca ccgagaggcc ggaagcaccg gtcagcatac ctatcccggg
    14581 cggacctttc ccgggaagaa gatggccgga cgctatggaa acgaacgcgt gaccgtgcac
    14641 aacttggaga tcgtgcgcat cgacgtggaa aacaatctca tcgccctcag gggggccgtc
    14701 ccgggtgcgc ccggcggtct gattatgctg agaaaagcgg tgagggtgaa ccttaagaag
    14761 gcgaagtaaa tacagttagg aaggaggagc cattgtggca gacattaaag ttctcgacct
    14821 cagcggacgg caagttggcg acttgaccct acgggacgat attttcaacg tgaccccgaa
    14881 caaagacctg ttatttcgat atatcgatat gcagctcgcc aacagacgac agggaacggc
    14941 ttgtacgaag actagagcgg aagtggccgg aaccggcaga aagccctttc cgcaaaagca
    15001 tacaggcaga gctcgtcaag ggacattgag gagcccgcac catagacacg gaggcgtggc
    15061 gttcggtccg aaaccgaggg attggagcaa aaaactgaac aaaaaaatga aacgagacgc
    15121 gttgaaatca gccctcagtg tccgttttca agagggaaac ctcatcgtct tggaggattt
    15181 gacgatgaag gcccccaaga ccaaagaact gaaagaactt ttggacaagt tcgaattgac
    15241 cggcctgaaa actctgttcg tgctgccgta caaaaaggtc gcctatgaaa acgttcttaa
    15301 gtccggaaag aacttaccca aaacgaaagt aatcatcgcg gacaacccgg gcctacccga
    15361 aggagaggtc tgtatcgatg gtctaaatgt cttcgacatc atcaaccacg aaaagctgat
    15421 cttgacgaaa ggcacggttg ccaaaattga ggaggtgctc ggcaatgtcc gttagattga
    15481 ccgatatgga tgttctactg cgcccgatca ttacagagaa gaccacgttt atgatgggag
    15541 atagcaagta caccttcgaa gtccacaccg acgccaacaa atatatggtg aagtctgtca
    15601 tagagaagat ctataacgtc cgggtagaca aggtgaacat cttgaacgtg aaaccaaaac
    15661 cgaagaagcg gggcgtgacg cggggaaaga cccgcggctg gaaaaaagcc gttgtgaaac
    15721 tcgtggacgg ttattcgata cccgagcttc agagtcttcg ataggtagaa aggggagaga
    15781 tctatgggac ttaaacgata caaaccgact acccccagcc gacgtttcac ggttcttccg
    15841 gacttttcgg agttgacgaa aaacacgaag ccggaaaaga ctctgctcga accgttgaaa
    15901 agcaaagcgg gccgaaacca tcacggtcgg atcacgacgc gtcatcaggg cggcggtcag
    15961 aagagacagt atcgcatcat cgactttaga agaaacaaga ccggcatccc ggcgaaagtg
    16021 aagtctatcg aatacgaccc gaaccgcagc gcccgtatcg cattgctcgt ctacaaagat
    16081 ggagaaaaac gctatatcct cgctccgaga gatttgaagg tcggcgacga attgatgagc
    16141 ggcccggatg cggaaattcg cttgggaaac gctttacccc tacagcgcat accggttggg
    16201 actttggtgc acgcggtgga gttcgtcccc ggtaaaggcg cccagatcag ccgatccgcg
    16261 ggcagttccg ttcagatcat ggcgaaagag ggcggcttcg cgctcttgaa aatgccgtcc
    16321 ggagaacttc gcaaggtcag agaagcctgt tacgctactg tcggtacggt gggtaacgag
    16381 gacaactcga acgtcgtcca cggaaaagcg gggagaaagc gccgactggg cattcggcca
    16441 accgttcgcg ggatgacgat gaacccgtgc gaccacccta tgggaggcgg agaagggaag
    16501 gccaagggcg gcaaacaacc gcagagccct tggggtacgc cggccaaagg gtataagacc
    16561 cgaagaggaa aacgcaattc cgacaagttc atcgttcgcc gaagaaccaa aaaataatag
    16621 gagggggtag gaaagatgag cagatcaacg aaaaaaggcc cctttgtcca tccgaaactg
    16681 ttgaaaaaga tccgggagtt gaaccaggcg ggaaggaagg aagttatcaa atcctggagc
    16741 agggcctcaa cgataattcc cgagatggtc ggacacacgg tggccgtgta caacggtatc
    16801 aaacatatcc cggtctacgt ttccgaaaac atggtcggac acaagctggg agagttctct
    16861 tatacgagac gattcggtgg ccatgccgat aaaaaagcca aaaaaggcga agtaaggtag
    16921 aggagtgact gtctatggcg cacgctaaac gttctatatt ccacgccgaa aggaaagagc
    16981 gcgaacgcga aacccccgtc atcgaggcgg cggcgaccgc gaagcatctt aggctttccc
    17041 cgcgcaaggc cagatcgatc gtcaatacca tacgtggcca aaaggtggac aaagcgttca
    17101 cgatcctcga gctgtcccct caaaaaccgg ccagaatcgt gctgaagctg ctgaagtccg
    17161 cagtggcgaa cgcggaacac aactttcagc tttccgtcga ccacctgatc gtatccgaag
    17221 ctttcgtcaa cgacggcccc aggatgaaga gggtatggcc gagaggtaga ggtagggcag
    17281 acatcctgca aaaacggatg agtcatatta cggtggtcgt gcaagaccaa tccaaagcat
    17341 cagaataaac tgagagaggt gattgtgacc gtgggtcaaa aagtccaccc gagaggcttt
    17401 cgtttaggag tatccaaaga ttggcaatcg gtatggtttg gcggcaagcg ctactccgag
    17461 ctcttgatag aggaccaagc cgtcagaaac catatttcta aaagaagata taaattgaaa
    17521 ctgcgaaatg gccagaccgt tgaggcgagc ggcgatatcg acgaggtcaa gatcgaacgg
    17581 ccgagtctga acaagatgat cgtcacgata gcgaccgcta aacccgggat tctgattggg
    17641 aaaaaaggtg cggagatcac cgaaatccga aacgagattc atgacctgac caaacgcgag
    17701 gtcatcatca acatcaagga gattaaaaca cccgaagtag ttgcccaatt ggtcgccgaa
    17761 aacgtggccg cgctgataga aaagagaact acccacaagc gagcgatgaa acgcgtgatg
    17821 aaaaacgcca tgatgaacaa ggcccttggc atcaaagtga tggtagcggg tcggttaggc
    17881 ggggccgaga tcgcgcggac cgagtggtat cgcgaaggtc ggattcctct acagacgctg
    17941 cgcgccgaca tcgattacgg gttcgcacag gcgaggacca aaaccggcat catcggggtc
    18001 aaagtgtgga tttaccacgg agacgtcacc aactagcgag gcgccctcgc cgcgcgagaa
    18061 gggaagagcg tttccttagc agcgatttct tgtgaatagg aggaaaagac catgttaatg
    18121 ccgaaaagag tgaaatatag aaagcaacag cgggggcgca tgcaggggaa ggccaaaggt
    18181 ggagcgaccg tcgaaatcgg agactggggg ctcaaagcgt tggaaccaca ctgggtaacc
    18241 gcccgacaga tcgaggcctg ccgcgttgcg atggttcgca cggtgaaacg ttcgggtaag
    18301 gttgtaatcc gtatctttcc cgacaagccc gtgtctcaaa gagcggcgga ttccagaatg
    18361 ggcaagggga agggaaacgt cgaaaagtgg gtcgccgtcg tcaaacccgg tagaatcctt
    18421 ttcgagatcg aaggagttcc cgacgaccta gccaaagctg ccttgagaaa ggcctctgca
    18481 aaacttccaa tcttaacgaa gatcgtcgca agacatgcca ttggaggtga ataattgtga
    18541 aggctcagga gattagaggt ctgacggacc aggagctggt acaaaagatg gaggcgttaa
    18601 aaaaagacct gatgaaccta cgcttccaat tagccttgaa acaattagac aataccaaca
    18661 agatgaaatc ggtcaaaagg gatatcgcta ggatcaaaac cattctgacc gaacgagagc
    18721 tcgcttcaca gaagtaaggg agggtgagga ggatgtcgag gaagatagaa ggaacggtgg
    18781 tgagcgacaa gatgcagaaa accgttgtgg tcagtacggt tcgttacgtc aaagacacgc
    18841 tcacctcgaa aaaagtgaag agaaccaaaa agtacaaagt ccatgacgaa gaagagatat
    18901 gccacgtcgg tgacgtgatc gaagcgcaag agtgtcgtcc cctcagccgt gataagcatt
    18961 ttatcctcac caggatcgtg cagcgcggga agtccggttt ggccgctgac ttggcgaact
    19021 cagaagagaa cgaagccctc caaagcgtgc gtcaaacgga ggtgaccggt cgatgattca
    19081 acaagaatca gtgttgacgg tggccgataa ttccggcgct aaaaaaatcc tggtcatccg
    19141 agttatgggc ggttcaaacc gcgccaacgg ctctatcggt gatatcgtca tcggcacggt
    19201 caaggaagcc ctcccgcata ccgacattaa aaaaggagag gtcgtccgcg ccgtcgttgt
    19261 ccgtacgcac aaagagatca gacgcccgga aggcacgtac atccgatttg acgacaacgc
    19321 ggcggtcatc gtcgataagc agaacgttcc gaaagggacc cgaatttttg gaccagtggc
    19381 tcgggagctg cgcgaaaaag gctttatgaa aatcgtctcc ctggcgccgg aagtgttgta
    19441 gggggaatga gaatgaaaaa atacaacttt cacattcgga aggatgacct cgtgctcgtc
    19501 atagctggcc gcgaccggaa aaagaaggga aaggtcatct ccgtgatccc gaaggatgcg
    19561 accgtcatcg tcgataaggt gaatacggtc aagaagcatc agaaaccaaa gcaacaggat
    19621 aaaagcggcg ggatcatcga taaaaatatc ccgattgccg tgtctaaagt tcaactgatt
    19681 tgccctagct gtgacaagca gactcgggtt ggatggaagg ccctcgaaga cggcacgaaa
    19741 gcccgtgtct gtaaacgatg cggggaaatg atcgatagaa aataaaggag gtggcgcttg
    19801 gtaggaaggt ccctgaaagg actttctatt atcaagttcc ctatgggaga aaagaaagaa
    19861 aaggtcgaga agcaacctaa agcgcagggc gaaaccaccc cggccgcaac cggaacgcaa
    19921 ggcgcaaaaa aacagaaaga gaagaaagaa acggcggata caaaacaccc gagcccggga
    19981 cctgacgtcg cctttgtcag ccctttgaaa gagcgctatc ggtcggtgac cgttccagaa
    20041 ctgatgaaaa agttccatta caaaaacccg ctccaagtcc cgaaagtgat gaaggtagtc
    20101 gtgaacatgg gggtgggaga gggaacgcga aaccgagacc tgttcgtgaa ccattcggaa
    20161 gagctgatgt tgattacggg ccaaaaaccg gtgattacga aagcgaaacg ggcgatttcc
    20221 aacttcaaaa tacggaaggg aatgcccgtc gggataaagg tcactctgcg cgggaaaaac
    20281 atggaaaact tcctgttcaa actgttcaac atcgtcttgc cgaaggtgcg agacttcaga
    20341 ggcctcaacc cgaactcctt tgacggtaag ggaaactact ccttcggact atcggaacag
    20401 atcatctttc cggagatacg cgcggaaaaa gtgaagcgga tacagggaat ggatatcatc
    20461 atcgtaacca gcgcgaagaa caacgaggaa tccaaagcgc tgttgagttc tcttggcctg
    20521 ccgttcaaaa aaggctgagg atggagggta aagatggcaa agaaatcgat ggttgcacgc
    20581 tggtctcgtc ccaagaagta taaggtcaga gagtattctc ggtgtaagat ttgtgggaga
    20641 ccccactcgg tataccggga ctttgggatt tgcagatgct gcttgagaaa gatggcgttg
    20701 gaaggcaaac ttccgggcgt caagaaatcc agctggtaag aaaggaggag caggctgtgt
    20761 ggagtgaccc tatcgctgat atgttaacaa gactgcgtaa cgcgaattca gtgttgaaag
    20821 agacggttga catcccggct tccaacctga aaaaggcgat ctgcgaagtc ctaaagagag
    20881 aaggctacat ccatgactat aaatacatgg aagacggcaa gcagggaatt atccgcatta
    20941 ttttgcggta caagggaagc cgtagaaaga gggagaaggt gctgaacggg gtggtaaaag
    21001 tctctaagaa cgggagacgc atctacttgt ctcacgacaa gatccttcct gtgaaaggcg
    21061 gtctcgggat cgccatcatc acaacctcca aaggagttat aaccgataag gaagcaagaa
    21121 gactgggagt cggcggcgaa gtcatcgcct acgtctggta gaggagatag gaggagtacc
    21181 atggctttag ataaagagaa gttgcacgtt ttatctcgac tcgcccgcaa accgattacc
    21241 gtgcccgcca acgtcaaagt cagcctcgaa ggccgcgcct tgaccgtcac aggcccccac
    21301 ggtacgctct ctcaaagcct gcacccgttt gtgagcgtga aggtcgaagg agggaagatc
    21361 acggtgcatc ccgatctgga gatgatgaaa agacctgccg aatccaagaa ctaccgggcg
    21421 atgaccggga cgtttttcgc cctcatccgc aacatggtcc tcggcgtcac ggaagggttt
    21481 cagaaggagc tgcacgtcgt cggcgtc
//
LOCUS       DAOPXN010000014        21100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2495_length_21100_cov_5.623378, whole genome shotgun sequence.
ACCESSION   DAOPXN010000014 DAOPXN010000000
VERSION     DAOPXN010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 21100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21100
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2495_length_21100_cov_5.623378"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /gene="trmFO"
                     /locus_tag="PKM49_01590"
     CDS             <1..1267
                     /gene="trmFO"
                     /locus_tag="PKM49_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HNY81539.1"
                     /translation="GCEAALYLADHDVPVTLYEMRPTCPTPVHQTGKLAELVCSNSLK
                     STDPNTASGLLKEEMRLLKSHLLEIAEACRLPAGSALAVDRERFSEMVGRRIADSTIR
                     VVREEYTELDGENYERTAVALCAGPATSDGLMGSISRFLKTSELFFFDAVAPIVDSET
                     IDFSHTFRADRYQEGEEGSYINLPLDEALYARFFRTLVEARTLDVPDFERKYLFERCQ
                     PIEEIARTGYDALRYGPMKPVGLIDPKTNREPYAVIQLRQENAAKNLYNLVGFQTRLS
                     WGEQKRLLRLIPALREVEIVRYGVMHRNTYLNTPRLFDAHLESKTCANLFVAGQMSGV
                     EGYMESAVSGRIVAGQILRRIRGQSLLEYPIETMVGALNAYITKADPLRPMYANFGIL
                     PPVKGKKKEIRALKSERALRVMKAFLAET"
     gene            1470..2477
                     /gene="fliG"
                     /locus_tag="PKM49_01595"
     CDS             1470..2477
                     /gene="fliG"
                     /locus_tag="PKM49_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNY81540.1"
                     /translation="MAESGYSGVRKASVLLLTLGTEKASRILKHMSDEDIERLTLELA
                     KLEKVNDNEKKSVLTEFVTNFRAREFMASGGIEYARDMLEKAVGVEKALEILERLTSN
                     LQVKPFDFLKKTDPMQLVNVLQNEHPQTIALILCYLLPKQAAQVIGSLPEGLKAEVVK
                     RIAMLDKANPEVVREVERVLERKVSTFVTQDFAQVGGLDTVVEFMNSLDRTTEKEILD
                     KLSETDPELSEEIRRHMFVFEDVVKLDDRSMQMVLREVDTKDLSRALKGASEEILNKI
                     VSNLSKRAGQLLKEEIEFLGPLRVVDVEEAQQKIVNVIRRLEESGEIFIARGGGEELI
                     V"
     gene            2470..3249
                     /locus_tag="PKM49_01600"
     CDS             2470..3249
                     /locus_tag="PKM49_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HNY81541.1"
                     /translation="MYNLIKRTLVTIDEKERRKINSGEPAQADEKADKLADGSEEKPR
                     EELIEMHPEVTLARERARSIIEDARRQAEQIRSEAQALTVAAQKQAEQKKVEMDQLLI
                     SKEREIENKWKAQYESAIRSVQALQQSLQKNKDEFIALGSQQILLLAKTVVEKMLFIV
                     LEDREAEVFERKIRELVHRALDYKKVVMRLNPKNLEFFSPTLLQSVKAIIPDVELRPD
                     PKIAPGGILVDTDFGTLDATIESQYQLFQEIVSQALVSDSP"
     gene            3291..4574
                     /locus_tag="PKM49_01605"
     CDS             3291..4574
                     /locus_tag="PKM49_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNY81542.1"
                     /translation="MRQTDFITLNGKVEKIIGLTIESAGPEVFLGEVCVIKNLNGERI
                     LCEVVGFKDNRIILMPLEDFEGVSLGCPVAGTKRTLDVGVGDDMLSRVFDGIGQPIDG
                     KPFFPKGYVSLNNSPPNPLVRARIKESLPVGVRAIDAFTTLGRGQRIGIFSGSGVGKS
                     TTMGMIARNTRADVNVIGLIGERGREVREFLERDLGEEGLRRSAVVVSTSDQPALMRV
                     KALYTSTSIAEYFRDRGLNVMLMVDSLTRWSMAQRELGLAIGEPPTTRGYPPSVFAGL
                     PKVLERAGNSDKGSITGIYTVLVEGDDFNEPISDTVRGIVDGHIILSRKLAEGNHFPA
                     IDVLASISRLMPEVADKEHLKASRALKDLISTYYAAKDLIDVGAYKDGSDIKIDQAKK
                     YISTINGFLQQEVADKAIFEETVNRLKKILQTIQS"
     gene            complement(5634..6848)
                     /locus_tag="PKM49_01610"
     CDS             complement(5634..6848)
                     /locus_tag="PKM49_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNY81543.1"
                     /translation="MKWNLIFTLTISALFFVASIIVYVSFLNIYTAQFDHELRQLSTE
                     VEKRILNLPALIRERLLTLGESRPSLYLSVEEVLQIFDLEGKKIYDIGIDVPDPPSFR
                     EGFSNENDFRFFTRILKNPRGIPVAFVRIGKNINGISRDLRGLVNLLGLLFAVIVTGS
                     WLLSNVLSAYVLKPLRSSYEQLKQFTSDASHEFKTPLSVMRASLDLIPTEGLEQGALE
                     KLGYMDLSVRKMQGLVDQLVQLAKSGGLAGSLKMEEITVEPFLTSIVDENRALIEQKA
                     LRVRVKADPKSQCHTASEPLRLILGNIVNNALKFTPEGGEILIGMIRRGKTNLFFVED
                     TGIGIEESEQKRIFERFYKVEGSRPAGSGSGMGLAIAKEYALLIGAQLSVKSHLNEGS
                     RFELMFTEKTVD"
     gene            complement(6874..7551)
                     /locus_tag="PKM49_01615"
     CDS             complement(6874..7551)
                     /locus_tag="PKM49_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY81544.1"
                     /translation="MKVLLVEDERTLSRLIYEALVHQLFTVETAFDGEEALFRIHNYT
                     YDAIILDILLPKRSGWEVLDEARKTGVQTPVLLLTALSQVEDRVRGLNTGADDYLAKP
                     FDIRELVARVQALIRRSVKTRNEGGLLRCADLEVDTIKKTVRRGDRLIDLTKKEYQIL
                     EYLLYHRGEVVTKETLEERLWGDEESLWSDVIRSHMKNLRKKLDQKHRRSLIRTVRGE
                     GYVCDCE"
     gene            complement(7598..8287)
                     /locus_tag="PKM49_01620"
     CDS             complement(7598..8287)
                     /locus_tag="PKM49_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81545.1"
                     /translation="MKRRSVSFLVISILLGCIVFGANMPVMAGQMRARAQGVAENAEY
                     LAQLTLAKVINELELTNEQLSKILAAAKDARTKLGGLKEAAEKQRQELLDLLVARNLE
                     GVKAFMSERKAPENPAVLLTQYVNTVKDIFTYAQGDKLSRLFNGFGGGFGPGNRSVNP
                     PSQTRGQNQNKRVPVPRQDQLRQGAKQQLRSFVAQPRMSAQCPQALNQLSKVFLTDWG
                     IDLLERFIAVK"
     gene            8580..9239
                     /locus_tag="PKM49_01625"
     CDS             8580..9239
                     /locus_tag="PKM49_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HNY81546.1"
                     /translation="MKKKMVSLQEFSEKIPSGATLLIGGFLGDGTPDLLIDEILKTDK
                     GDFTVIANDTAFPNQGIGKLITQKRVKKAIVSHIGTNPDTQKQMIEGSLVVELVPQGT
                     LAERIRAGGFGLGGILTPTGLGTIVENGKSKIQIGGREYLIESAIKADFALLKAKKAD
                     WYGNLTFAYTARNFNTFMAFAGDCTIAEIDELVPIGGLNPEDVVVPHAVVHFLVRRSR
                     S"
     gene            9236..9901
                     /locus_tag="PKM49_01630"
     CDS             9236..9901
                     /locus_tag="PKM49_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008801571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HNY81547.1"
                     /translation="MIAEEKAKGYIARRVAKEFEDGDVVNLGIGIPTLVANYIPSGIQ
                     IFLHTENGLLGMGPNPSAGYEHPNLRNAGSSSVSFLPGSAIFDSAVSFGLIRGGHLDA
                     TVLGALEVDEKGLLANWMVPGKMIPGMGGAMDLVTGARRVIVAMTHVNKNGSPKIVKS
                     CMLPLTSIRKVDLIVTEYAVFKVSETGLVLTEYSEESSVEEIKGLTAAHFEIDPQLKV
                     IER"
     gene            9952..12822
                     /locus_tag="PKM49_01635"
     CDS             9952..12822
                     /locus_tag="PKM49_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY81548.1"
                     /translation="MKLSKYLFVLACFLAAFGLTVFHVLLARDREWELSRAHQELKQQ
                     ASIVEQHLLDKTSNIDSLLNYIRKEVAEERDVATLRALLINLAALNADYVDLIAVTNR
                     EGDLFTSSQIPFKEQNISDRSYFSFHQNNGFREMLISHPLLGRAIEKMYIPMSLRLQD
                     DRSGFTGIALCAVKPEHFQTFFSSVLSDTDLVMILALECGDVLASSGVKDESNIQLFN
                     NRVFQEIVKGRDLGKEGVRFLEEKRRMVVGTKIGSYPLYFMIAKEESVILSKHYSRAL
                     LVGGFASGLFTALFLFHWAVFWVLKKESEKTHTLRKGASENLLFFSAIPEGAWDWRVR
                     EEKIVVNDAFYKLLGYEGVQTISNLQEWLALIHPEDVPLVERAFQRHYELKQPFSLEF
                     RVKARSGDWRWTASKGILVESDDDGNPVRMIGILSDAQPVKELDALVDKQREELIHTE
                     SRLAKLIHADEDRELFLEILLQGLRALQEPNSLSGVVSKLLNLCRELVDAKDGYVAFL
                     NPNKETQQIMALQAGINPTHILTFPVFVSELESSVYKGKRVRYENNVEIGWIREPGNP
                     PIFLENIIISPIFSNGEVIGLLALANRIGGFKSLGSTFLDTFSLLISTAMRINRKGEE
                     LPSSSERSGEMERLLSSGIEAAPLGPMVLTDLIKEAVGDLREAATGSVSFHCDIHPEV
                     PVVLEDDTASVKRVVGLILKTVTNHRTKEEIVVSAYPSRPLEKEDSNIRWIDIVVQER
                     ENESQTKGYSSANGEAEVVKSESEKDKRLLAKELQQAQEAAKQVGGRVELHAEKGLGI
                     VVTLKLPFRLFKHPHQIKESPETTRNGEISGVTILVAEDDPINRRIVRALLERRGWRV
                     IEVEDGEQAVNQWRTRQPDLILMDIRMPKIDGYEATKMIREEEKTRGAGTVTPIVALT
                     AYAMQEDREKCVALGMNEYVSKPIQKEELIAKILQLIGRSEA"
     gene            12908..14599
                     /gene="ggt"
                     /locus_tag="PKM49_01640"
     CDS             12908..14599
                     /gene="ggt"
                     /locus_tag="PKM49_01640"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008698877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNY81549.1"
                     /translation="MQSKKPVVLGLVAAVLLTSAVFGANPSNLYGRGARATNGVVAAA
                     KPEASQVGVDILKKGGNAVDAAIATAFALGVLEPNASGLGGGGFMIIKLVDMAEPVII
                     DFRECAPLRATPDMYKFNARNQVIGNENAIGGKASGIPGEVAGLLFALERYGTMTRAE
                     VIAPAIEWAEKGIPVSANLRQIMMDNYMKLLEFDATAKIYLNDDLPYEVGETLYLKDL
                     ARTLRKIAQEGASAFYTGEIARQIVEEVGKRGGLFTLEDLASYEVKVRTPVKGTYRGY
                     QILSTPPVSSGGAHLIELLNILENYDLSAIGANTPLWAHLWSEAMKLVFADLSKYMAD
                     TDFITVPLEGITSKAYARERAKEISLTGPMPTVIAGDAWANQTGSTTHLSVADKEGNM
                     VAITKSINSFFGSGVVIPGTGILMNNHMDDFDLRPGSANSIEPKKRPLSSMCPTLVMT
                     PDGRPFMAIGSPGAFRIITTIAQVISGVIDHGMDINTAILAPRLFRTHSGPMSVEGRI
                     SLATYEALRKMGHELDVRGDFDNFFGGVQAVLYLHDQNVLVGGADPRRDGQAIGY"
     gene            complement(15057..16460)
                     /gene="dnaA"
                     /locus_tag="PKM49_01645"
     CDS             complement(15057..16460)
                     /gene="dnaA"
                     /locus_tag="PKM49_01645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNY81550.1"
                     /translation="MENQNRNTQIESAPTDFFAYLRSNVDKKHWDLWFKSLELLEILT
                     DKNLVVLQTANLFVKDWILTRYGKVLSSAVKSFFGPAFSFELRDALLTDEPENGEVKS
                     TPTRFTGKKPLEICSLNNQYTFEDFIAGGGNLLAFQSAKSVSAAPGRYNPLFIFGDVG
                     LGKTHLLQAIGHEVRSQFPEKKVMYLSSEQFMNELVQSIKQDRIEQFRNKYRHSIDIL
                     LIDDIQFLIGKMGIQNELFHTFNLLFDANKQIAFCSDRTPKDLGTFHERLISRFQMGL
                     VVELNAPDIETRKRIIEKISKREKITMSPSVVSYLAHAIKDNIRKVYGTIVKLIMMNQ
                     LQGKSIDIHTLKEVLEEDRAALSGADAQIIRLSERERYFSIVEKAFGFTKADLTSDSR
                     KKEVSEARKVAIYVAHKQMRIPVLELSKWFEKTHSTVHYAIAKTQENYDKRCASTIKL
                     VDSLKLELSRMRSSVQA"
     gene            16669..16803
                     /gene="rpmH"
                     /locus_tag="PKM49_01650"
     CDS             16669..16803
                     /gene="rpmH"
                     /locus_tag="PKM49_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNY81551.1"
                     /translation="MKRTYQPSRAKRKRTHGFLVRMRTTNGRKVIANRRRSGRKRLAV
                     "
     gene            17199..17426
                     /gene="yidD"
                     /locus_tag="PKM49_01655"
     CDS             17199..17426
                     /gene="yidD"
                     /locus_tag="PKM49_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNY81552.1"
                     /translation="MTQLIRFYQNNISPLKPSCCRFYPTCSHYALAVYERFGFLRGSY
                     LALKRLSKCHPLHSFGVDLPPERLSLFEKKR"
     gene            17463..18869
                     /locus_tag="PKM49_01660"
     CDS             17463..18869
                     /locus_tag="PKM49_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HNY81553.1"
                     /translation="MKRAFAIAILFFVFALTAFSKNEFSLVYVDGFLRIQTRISQLTF
                     NTREGALNEMALTMESYNKIYGYGEDGFDLFLNGKKITPDGVSINGRDAASFAERTVS
                     LEENEGVVIDFHYGNMIKRVTVPYGPYYEMSIELLGEIPEGLTLSLPRLADFNPNDNT
                     DRVNETGKVFTSYYQRTVALAVWAIERGRTAFRLDGSKHANELPIAYDGVKIKGYVGP
                     AKKMTFLQTVFPEEYVWLSRIVNSYPKASSWYDPLFYLLVYLMDWLYYLTKNYGWALI
                     LYTIIVRMALFPLAYSQTKSMITRKKLEKNAEYIKIMKIQDNQKKQKELMDFYKKNKA
                     SPAAGCLPALLQLPIFLLLYAVIRYESELFAFGPTFFFWRDLSIGGFKENILIVIISF
                     VINFFNTLITSSDTKQAKQGLLMAGVFPFLFITLATGLQIYWVTQSVIQWAITFWMFK
                     RSDTQGITIREFLLSFKK"
     gene            18882..19586
                     /gene="jag"
                     /locus_tag="PKM49_01665"
     CDS             18882..19586
                     /gene="jag"
                     /locus_tag="PKM49_01665"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNY81554.1"
                     /translation="MKEFVVSAKTVEEAVDSVKRENGFREGEFEYRVEDPGSRGFLKI
                     GSRNASISVIVRTEYYVRKVKEFIETVLSFSKYAPQSLAVKAEHRDAKIFVHLDGAHL
                     GRMIGKHGKTISALQHLATIFINRITDTKMTVLIDVGDYKDRRKELIKRIARNKALTV
                     IQTGKSVQLDPMFAFERRIVHETIKSISGVQSFSKGLEPYRYVVIEPEKKGRRKHRTS
                     PKGGVIDFEDHRSQAG"
     gene            19558..19914
                     /locus_tag="PKM49_01670"
     CDS             19558..19914
                     /locus_tag="PKM49_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNY81555.1"
                     /translation="MKIIVRKPDEVPVVRYDNPEKAWKVTKRVMVGEKEGARHFVTRV
                     FTIEPGGYTPKHTHPWEHEILVIKGKGTVFTEEGDREVEPGTALFVPPGSVHQLSNRS
                     DENFVFACVVPMEGET"
     gene            19994..20317
                     /gene="acpP"
                     /locus_tag="PKM49_01675"
     CDS             19994..20317
                     /gene="acpP"
                     /locus_tag="PKM49_01675"
                     /inference="COORDINATES: protein motif:HMM:NF002150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY81556.1"
                     /translation="MTKNEVFGQIKKIIVEKFNLEEEEIKLGSSFVEDLGADSLDLVD
                     LVMALEDEFGIKIEENDTDTLKCIEDVVNFIYEKLGGAADEEDVTEDVDEDSDEKVNV
                     DELDE"
     gene            20345..20680
                     /locus_tag="PKM49_01680"
     CDS             20345..20680
                     /locus_tag="PKM49_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81557.1"
                     /translation="MQRRHRSRKGFSLLLELLLAAALSFFCFTLLCSWFERNARIENT
                     RKRIREARDTFLFQYALLENGYSASEKEPVRRYALGQETVIEIYEISLPELNRSIECG
                     IIIQKESGE"
     gene            20677..>21100
                     /gene="argS"
                     /locus_tag="PKM49_01685"
     CDS             20677..>21100
                     /gene="argS"
                     /locus_tag="PKM49_01685"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNY81558.1"
                     /translation="MMDTTPLEVLKESIFHLLRAGHDLPAGDPVPFRIGPSPERKFGH
                     FSTNVAMAYAKVLKTPPRQIALSLVKGLEALPDIESVSIEGAGFINMLLKPSYFQERV
                     LRILQGEYTANFSLFPPDPIQIEFVSANPTGPLTVASAR"
BASE COUNT         5313 a         5108 c         5707 g         4972 t
ORIGIN      
        1 cggatgcgaa gcggcgttat acctagccga tcacgatgtc ccggtgacgc tttacgagat
       61 gaggccgacc tgtcccactc cggtccacca aacggggaag ctcgccgagt tggtttgctc
      121 taattctctc aaatcgactg acccaaacac cgcctccggt ttgctcaaag aagagatgag
      181 gcttttaaag agccatctgt tggagatcgc cgaagcgtgt cgcttgcccg ctggctcggc
      241 cttggcggtc gaccgggaac ggttttcgga aatggtcggt agacgtatcg ccgactctac
      301 gattcgggtg gttcgggagg aatacaccga actcgacggt gagaattatg agcgaactgc
      361 tgtggcgctt tgcgccggcc cggccacttc cgatgggctc atggggtcca tctcccggtt
      421 tttgaagact tccgaactct ttttcttcga cgccgtcgcg cctatcgtgg atagcgaaac
      481 gatagacttc tcgcatactt ttcgggccga tcggtaccag gaaggagaag aggggtccta
      541 tatcaacctt cctctagacg aagccctcta tgcgcgcttt tttcgtacct tggtcgaagc
      601 gcggacccta gatgttcccg attttgaacg gaaatacctt ttcgaacggt gccagccgat
      661 cgaagagatc gcgcgcaccg gatacgacgc cttgcgatac gggcctatga aaccggtcgg
      721 tttgatcgat ccgaaaacga accgagaacc ctatgccgtg atacaattac ggcaagagaa
      781 cgcggcgaaa aatctataca acctggtcgg ttttcagacg cgtttgagct ggggggagca
      841 aaagcggttg cttaggctga ttcctgctct tcgagaggtg gagatcgtcc gttacggagt
      901 catgcatcgg aacacctacc tcaatactcc gagattgttc gatgcccatt tggaatcgaa
      961 aacgtgtgcg aacttgttcg tggcggggca aatgagtggt gtggagggat atatggaaag
     1021 cgcggtcagc gggcggatcg tagcgggaca aatcttgcgg cggatccgtg gacagtcgct
     1081 tttagaatat cccatagaaa ccatggtcgg cgccctgaac gcgtatatta ccaaagccga
     1141 tccactccgc cctatgtatg cgaattttgg aattctcccg cctgttaagg gcaagaaaaa
     1201 ggaaatacga gctttgaaat cggagcgagc gttacgcgtg atgaaggcct ttctcgcaga
     1261 aacctagaca taagaatcct aaactccgtc gatctttaag gaatagaaaa agttcggtga
     1321 ctatagggcg aacgatggga aaaaccgtcc gcccgctcta taattcgtgc tataatgcct
     1381 tacggttggt tcctcggcgt tcgcggaatc ggaaggcgag ttcgggccct ctaaaaggaa
     1441 agcggcccac cgggatcgag ggagagagta tggcagaaag tgggtattca ggagttcgga
     1501 aagcatcggt tctattgttg acgctcggca cggagaaagc ttcgaggatt ttgaagcata
     1561 tgagcgacga agacatcgaa cgtttgactc tggagctggc gaaactggag aaagtcaacg
     1621 acaacgagaa gaaatctgtg ctcaccgagt tcgtcaccaa ctttcgcgct cgggagttta
     1681 tggcctccgg gggcatcgag tatgcgcggg atatgttgga aaaagcggtc ggcgtcgaaa
     1741 aggcgttgga gatcctcgag cgattgacct ccaacctcca agtgaagccc ttcgactttt
     1801 tgaagaagac ggacccgatg cagctggtga acgtcttgca gaacgaacac ccgcagacga
     1861 tagcgttgat cttatgctac ttgctcccga aacaggcggc ccaagtgatc ggatcccttc
     1921 cggaggggct gaaagccgag gtggtgaaac ggatcgccat gctcgacaaa gcaaacccgg
     1981 aggtggtgcg cgaagtggag cgcgtgctcg agcgaaaggt atcgaccttc gtgacccaag
     2041 atttcgctca agtgggcggg ttggatacgg ttgtcgagtt tatgaacagt ctggatcgca
     2101 cgaccgagaa ggagatactg gacaagcttt ccgaaacgga tcctgagctg tcggaagaga
     2161 tccggcggca tatgttcgtg tttgaagacg tggtgaaact cgacgacaga tcgatgcaaa
     2221 tggttctccg cgaggtggat acgaaagatt tgagccgggc tttgaaaggg gcatcggagg
     2281 agattttgaa caagatcgtg agcaaccttt cgaagcgggc cgggcagctg ttgaaggaag
     2341 agatagagtt tttgggcccc ttgcgggtgg tagacgtgga ggaagcgcaa caaaagatcg
     2401 tgaatgtgat ccgcaggctg gaagagagcg gcgagatttt cattgcccgc ggcggaggag
     2461 aggagctcat tgtataacct gatcaagcga accctcgtca ctatcgatga aaaagagagg
     2521 agaaagatca actcgggcga acccgctcag gcggatgaaa aggccgataa actcgcggac
     2581 ggtagcgaag aaaaaccccg ggaagaactg atcgagatgc atccggaagt gactttggcc
     2641 cgagaaaggg cgaggtctat catcgaggac gcaaggagac aggccgagca aatccgcagc
     2701 gaagcgcagg ctttgaccgt cgcagcgcaa aaacaagccg aacaaaagaa agtggagatg
     2761 gaccaactgc tcatcagcaa agagcgagag atagagaata aatggaaggc gcaatacgag
     2821 agcgcgatac gttccgttca ggctttgcag caaagccttc aaaagaataa agacgaattc
     2881 atcgccctcg gttcccagca gattcttctc ttggccaaaa ccgtcgtgga aaagatgctg
     2941 ttcatcgtcc tcgaagacag agaagccgag gtgtttgaac ggaagattcg agagctggta
     3001 caccgcgccc tggactataa aaaagtcgtg atgcgcctca atccgaagaa cttggaattc
     3061 ttctcaccaa cgctgttaca gagcgtgaaa gctatcattc cggatgtcga gctgcgcccc
     3121 gatccgaaga tcgcgcctgg aggtatcctc gtcgacacgg acttcggcac cctggatgcg
     3181 acgatcgaaa gtcagtatca gctcttccaa gagatcgtct cgcaggcgct tgtgagcgat
     3241 agcccttgaa ccccgaagtc ttcgctccga tccagtggct tcaagaaact atgcggcaga
     3301 cggactttat cacactgaac ggaaaggtgg agaaaatcat cgggctgacg atcgaatccg
     3361 ccggtccgga ggtgttccta ggcgaagtct gcgtcatcaa gaacctaaat ggagagcgaa
     3421 tcctctgtga agtcgtgggg ttcaaagaca accggatcat cctgatgccg ttggaagact
     3481 tcgagggggt ttcactcggc tgtccggttg ccggtacgaa acggacgctg gatgtgggag
     3541 tcggcgacga catgctgtcc agggtcttcg acggtatcgg ccaaccgatc gatggaaaac
     3601 ccttctttcc gaaaggttac gtatctctca acaattcgcc gccgaacccg ctcgtccgtg
     3661 ccaggatcaa agaatcgtta ccggtcggcg tgcgcgcaat cgatgcgttc accacgctag
     3721 gacgaggtca gcgcataggg atattctccg gaagtggcgt cggaaaatcg acgacgatgg
     3781 ggatgatcgc ccgcaacacg cgtgccgatg tcaacgtgat cggcctgatc ggtgaaagag
     3841 gccgggaagt ccgagaattt cttgaaaggg acttgggaga agaggggttg cgccgcagcg
     3901 cggtggtcgt ctctacttcg gaccagccgg cgttaatgag ggtgaaggcg ctttacacga
     3961 gcacctccat cgccgaatac tttagagacc ggggtttgaa cgtgatgctt atggtcgatt
     4021 cccttacccg ctggtcgatg gcgcagcggg agttgggttt ggctatcgga gagcccccca
     4081 ccacgagggg ttacccgcca agcgttttcg ccggattgcc gaaagtgctg gagagggcgg
     4141 gaaactcgga taaagggagc attaccggta tctacacggt tttagtggaa ggcgacgatt
     4201 tcaacgaacc catttcggac accgtccgtg ggatcgttga cgggcatatc atcttgtccc
     4261 ggaaactcgc ggaaggcaat catttcccgg ctatcgacgt gttggccagt atcagccgtt
     4321 tgatgccgga agtagctgac aaggagcacc tgaaggcctc acgggcattg aaagacctga
     4381 tctccaccta ttacgctgcc aaagacctca tcgacgtcgg cgcctacaaa gacggcagcg
     4441 acatcaagat cgaccaagcg aaaaagtata tctccacgat caacggtttc cttcagcaag
     4501 aggtcgccga taaggcgatc ttcgaagaaa cggtaaatag gttgaaaaag attctgcaaa
     4561 cgattcagag ctgaaacgtc cttttcaaaa tagcgcttta tcgtaagaaa tcacaacaca
     4621 cacataacac gcagacgcta cggtttctct agaaaaagag tcgctactcc cgtttgccct
     4681 cgaaagaacc tgtgcgggtc aggttatggg gtgatataac caataggttt atcgcggggt
     4741 aactaacggg gccccgcgat gctgcgcacg ggaacgctcc ctgtgaaaat ataaaaaacg
     4801 tgctgaacta aagtttggcg ttcccaggcg ttcacaacgc cttcccaaga ctttcccaag
     4861 aatcttttct ctgtgttctc tgtgccctcc gtgcgaaccc gttttttttc tttggaatga
     4921 atgaaaaggg tatcgcgccg tcgcgaaaag aaggagcgct tacgccgcga tgcccgttta
     4981 tgttatcgcg gggtaaccag cgggctgact tcggaagccc acgaagtctc gcgatgctgc
     5041 gttcgggaac gctccctgtg aaaatataaa aaacgtgctg aactaaagtt tggcgttccc
     5101 aggcgttcac aacgccttcc caagactttc ccaagaatct tttctctgtg ttctctgtgc
     5161 cctccgtgcg aacccgtttt tttatcttcg gaattaacga aaagggtatc gcgccgtcgc
     5221 gaaaagaagg agcgcttacg ccgcgatgcc ctcgcacaaa agcgtatcgc gccgtcgcaa
     5281 aaagaagaga cgcttacgcc gcgatgcccg tttgtgttat cgcggggtaa ctaacggggc
     5341 cccgcgatgc tgcgcacggg aacgctccct gtgaaaatat aaaaaacgtg ctgaactaaa
     5401 gtttggcgtt cccaggcgtt cacaacgcct tcccaagact ttcccaagaa tcttttctct
     5461 gtgccctccg tgcgaacccg ttttttttat ctttggaacg tatgaaaggg gtatcgcgcc
     5521 gtcgcaaaaa gaagagacgc ttacgccgcg atgattacac ggactaaaaa ctgtgcgatc
     5581 gattctggtc ttgtttcttg attacacccc aaagcttgat ccacacaagt cccttaatcc
     5641 acggtttttt cggtgaacat aagctcgaat cggcttcctt cgttcaaatg gcttttgacc
     5701 gataactggg ctccgataag taaggcgtat tccttagcga tcgccaggcc cattcccgaa
     5761 cccgaacccg ctggccggct cccttcgacc ttgtagaagc gttcgaagat ccgtttttgt
     5821 tcggattctt cgattccgat cccggtgtct tccacgaaaa aaaggttagt tttccctcgt
     5881 ctgatcattc cgatcaaaat ttctccgccc tcaggcgtga acttcagggc gttgttgacg
     5941 atatttccga gaatgaggcg caagggttcg ctcgcagtat ggcattgaga cttggggtcg
     6001 gctttaacgc gcacgcgtaa ggctttttgt tcgatgagcg cgcggttctc atctactatg
     6061 gatgtgagga agggctctac ggtgatttct tccattttta aggatcctgc gaggcctccg
     6121 ctttttgcga gctggacgag ttggtccact agaccttgca ttttccgaac agataggtcc
     6181 atatacccaa gtttttcgag cgcgccttgt tcgagacctt ctgtagggat cagatccagc
     6241 gaagccctca taaccgacag cggggtcttg aactcgtgag aggcatcgct tgtgaactgc
     6301 ttgagctgtt cataactcga tcgtaagggt tttaagacgt aagcagaaag aacattactc
     6361 aacagccagg agcctgtgac gatgaccgca aatagcagcc ccaataggtt aactaacccg
     6421 cgaagatcac gggagatccc gttgatgttt tttccgatac gaacgaaagc gacgggtatc
     6481 ccgcgtgggt ttttcaggat tctggtaaag aagcgaaagt cgttttcgtt cgagaagccc
     6541 tctcgaaacg agggcggatc gggaacatcg atgccgatat cgtaaatttt cttcccttcg
     6601 aggtcgaaga tttggagaac ttcttccacc gaaaggtaga gtgaggggcg gctttctcct
     6661 agtgtgagca acctctccct gatcaaagcc ggaaggttca gaatacgctt ttccacttcc
     6721 gtcgagagct gtcgaagctc gtggtcaaat tgcgcggtgt agatgtttag aaaggagacg
     6781 tagacgatga tcgaagccac gaagaagagg gccgagatcg ttagggtaaa gatgaggttc
     6841 cacttcaacc gcgcccttct gaatagggcg gtatcactcg cagtcgcaaa catagccttc
     6901 gccccgaacg gttctgatca acgacctccg atgcttttga tcgagctttt tccgcaggtt
     6961 tttcatatgg cttcggatca catcggacca caaagactct tcgtcgcccc acaaccgttc
     7021 ttctaaggtt tctttcgtaa cgacctctcc gcggtgatac aggaggtatt ccaagatttg
     7081 gtactctttt ttcgtcaagt cgataaggcg gtcgccgcgt ctgactgttt tcttgatggt
     7141 atcgacctcc aaatccgcgc atcgcaacag tcccccttcg tttcgggtct tgacgcttct
     7201 tcggatcaac gcttgaacgc gcgcgaccag ctcgcgaata tcgaacggct tcgcgagata
     7261 atcgtccgcg ccggtattca atccgcgtac ccgatcttcc acttgggata aggccgtcaa
     7321 tagcaaaacg ggggtttgca cccccgtttt gcgcgcttca tcgaggacct cccaacctga
     7381 ccgtttcgga agcaggatat ccaatatgat cgcgtcgtaa gtgtagttat ggattctgaa
     7441 cagcgcttcc tctccatcga aagccgtttc gacggtgaaa agctgatgga cgagcgcctc
     7501 gtaaatcaag cgcgataggg ttctttcgtc ttccactaac aggactttca acggtctccc
     7561 ctttcggtcg ggcagcaccc caatcgtgtg agcgaggcta ttttacagcg atgaagcgct
     7621 ccaacaaatc gattccccaa tcggttaaga aaaccttcga caactgattc agcgcctgag
     7681 ggcactgcgc gctcatacgc ggttgcgcta cgaagcttcg taactgttgc ttcgcgcctt
     7741 gcctcaactg atcttgacgt ggaacgggca ctcgcttatt ctggttttgg ccccgtgttt
     7801 gactaggcgg gttgaccgaa cgattccctg ggccaaaacc tcctccgaac ccgttgaaga
     7861 gccgagaaag cttatctcct tgcgcgtagg tgaaaatatc tttcaccgtg ttcacgtact
     7921 gggtgaggag aaccgcaggg ttttccgggg cttttcgttc ggacataaag gctttaaccc
     7981 cttcaaggtt acgggctacc agcaaatcca gcaactcttg tcgttgtttt tctgccgctt
     8041 ccttcaaccc gccgagtttg gtccgcgcgt ccttggctgc cgcaaggatc ttgctcaact
     8101 gttcgttcgt cagctccagt tcattgatga ccttggccaa cgtcaattgg gctaggtatt
     8161 ccgcgttttc agccacaccc tgcgcacgcg ctctcatttg tcctgccata acaggcatat
     8221 tcgccccgaa cacaatacaa ccgagtagga tagagatgac taaaaacgat acacttcttc
     8281 ttttcatacg atcgcctcct ttttctgcgg ggagtatacc tgacgcaccg tgaagaaacc
     8341 gtggattttt tatcgtcttg atgccctccc cggtcacctt cgtctccctg ggagacgaaa
     8401 agacagcagg gttatcgggc gtttagagtg attgacaatc tgtcattttt tctgtatgat
     8461 atattatcac agacgaagaa tatttttatt ggaggtgagc tctgtcaggg atttaggggt
     8521 cgctcattcc gtacttatcg cggcgatgca ttcctactaa acactgatac ggtatcaata
     8581 tgaagaaaaa gatggtctct ttgcaagaat tctcggaaaa aataccttct ggcgccaccc
     8641 tgttgatcgg cggctttttg ggagacggca caccggattt gctgatcgac gagatattga
     8701 agactgacaa aggtgatttc acggtgatag cgaatgacac ggcctttccc aaccaaggaa
     8761 tcggaaagtt aatcactcaa aagcgggtca agaaagcgat cgtatcccat atcggcacga
     8821 acccggacac acaaaagcag atgatcgagg gttccttggt tgtagaactg gtaccccaag
     8881 gcacattagc cgaaagaatc cgggcgggcg ggtttggatt gggcggcatc ctcacgccaa
     8941 cgggattggg cacgatcgta gagaacggaa agtcgaagat ccaaataggc gggagggagt
     9001 atctcataga aagcgcgata aaggcggatt tcgcccttct caaggccaaa aaggctgatt
     9061 ggtacggcaa tctcacgttc gcgtacaccg caaggaattt taataccttt atggctttcg
     9121 ccggcgactg tacgatcgcc gaaatcgacg aattggtacc catcggcggg ttgaatccgg
     9181 aagacgtggt ggtaccgcac gcggtagtgc actttctggt aaggaggagc cgatcatgat
     9241 agcggaagaa aaagccaaag ggtatatcgc ccgccgggtc gcgaaagagt ttgaggatgg
     9301 agacgtcgtc aatttgggga ttggcatccc aaccttggtg gcgaactaca ttccatcagg
     9361 gatacagatt tttctgcaca cggagaacgg tctgttggga atggggccga atccgtcggc
     9421 aggatatgaa cacccgaacc ttcgcaatgc ggggagttcg agcgtgagtt ttctgcccgg
     9481 gagcgccata ttcgactccg ccgtttcctt cggcctgatt cggggcggcc acttggacgc
     9541 cacggttttg ggcgcgctag aagtcgacga aaaagggctt ctggccaatt ggatggtgcc
     9601 gggtaagatg atccccggta tgggcggggc gatggacctc gtgacgggcg cccgacgggt
     9661 gatcgttgcg atgacgcacg tcaacaaaaa cggctcacca aagatcgtga agtcgtgtat
     9721 gctgcccctc acctccatta ggaaagtcga tctgatcgtg accgagtatg cggtctttaa
     9781 agtttcggaa acaggcttgg tgctcaccga gtattctgaa gagagttcag tggaagagat
     9841 caaaggcttg acagcagccc attttgagat tgaccctcaa ttgaaggtca tcgaacgata
     9901 atacgcgttc taggaatcgc gtttccaagg gcgcaagaga ggttgtattt attgaagctt
     9961 tcaaaatatc tctttgtttt agcttgcttt ttagccgcgt ttggtctgac ggtatttcac
    10021 gttttattgg cgcgcgatag agagtgggag ctgtcgcgcg ctcatcaaga gctgaagcag
    10081 caagcttcca tcgttgaaca gcacttgctc gataagactt ccaatattga tagcctactc
    10141 aactatatac gtaaagaggt tgcagaagaa agggacgttg caacgttacg agcgctgctg
    10201 atcaacttgg cggccctgaa tgccgattat gtcgatctaa tagcggtcac gaatcgagaa
    10261 ggtgatttgt tcacttcaag tcagattcct tttaaggagc agaatatctc agaccgttcc
    10321 tatttctcct ttcaccagaa caatggtttt cgagagatgc tgatctccca tccgcttttg
    10381 ggaagggcta tagaaaagat gtacattcca atgagtttac ggctccaaga cgaccgaagc
    10441 ggttttacag gcatcgcgct ctgcgcggta aaacccgagc actttcaaac tttcttctct
    10501 tcggtcttga gtgataccga tctggttatg atactggcat tggagtgtgg ggacgtttta
    10561 gcctcatcag gggtaaaaga tgagtcaaat atccaacttt ttaataaccg ggtatttcag
    10621 gaaattgtca aagggcggga cttaggaaaa gaaggcgtta ggtttttaga ggaaaaacgc
    10681 cggatggtcg tggggacaaa aatcggatcc tatcctctgt attttatgat cgcgaaagag
    10741 gagagcgtga ttttatctaa gcactattct cgcgctcttc tagtcggagg gttcgcgagc
    10801 ggtctattta cggcgctttt cctctttcac tgggccgtct tttgggttct caaaaaggag
    10861 tccgaaaaaa cccacactct taggaaaggg gcttccgaaa acctcttgtt tttttcggcg
    10921 attcccgaag gcgcttggga ttggagagtg agagaggaaa agatcgtcgt gaacgatgcc
    10981 ttttataagt tgctagggta cgagggggtt caaacgatat ccaatctaca agaatggttg
    11041 gctttgatcc acccggaaga tgtgccgctc gtagaaagag cgtttcaaag acattatgag
    11101 ttgaagcagc ccttttcctt agaattccgt gtgaaagcgc gctcgggaga ttggcgatgg
    11161 accgcttcaa aagggatact cgtggagagc gatgacgacg gtaatcccgt tcgtatgatc
    11221 ggaatcctat ccgacgcgca accggtgaaa gagctggacg ctctggtgga taaacaaaga
    11281 gaggagctga ttcatactga gtctcgactt gcgaagttga ttcacgcgga cgaggaccga
    11341 gaactcttcc tcgaaatctt gctgcaagga ctgcgggctc tacaggaacc gaattctctc
    11401 tcgggtgtgg tatcgaaact cttgaatctg tgtcgagagt tagtggacgc gaaggacggc
    11461 tacgtggctt tcctcaaccc caataaagaa acgcagcaga ttatggcact gcaggccggg
    11521 atcaatccga cgcacatctt gactttcccg gttttcgtaa gtgaactgga aagttcggta
    11581 tacaaaggaa aacgcgttcg ttacgagaac aatgtagaaa ttggatggat ccgagaacca
    11641 gggaatcccc cgattttttt agaaaacatc atcatctccc cgattttttc caatggagag
    11701 gtaatcggtt tactggcgct tgcaaatcgt ataggtggat tcaaatcgtt gggttcgact
    11761 tttttggata ccttttctct tctcatttct accgcaatga gaatcaaccg caagggagaa
    11821 gaattaccat cctcttctga acggtcgggg gaaatggagc ggttgctgag ttcgggaata
    11881 gaagccgcac cactaggccc tatggtttta actgatttga tcaaagaagc cgtgggcgat
    11941 ttgcgcgaag ccgcgacggg atccgtatct tttcattgcg atattcatcc ggaggtacca
    12001 gttgttttgg aagatgatac cgcttcggtt aagcgcgtag tcggtcttat cttgaaaacc
    12061 gtgactaatc accgaaccaa agaggagatc gtagtctccg cttacccatc gcgccctctc
    12121 gaaaaagaag actcaaatat ccgttggatt gatattgttg tccaagaaag agagaacgag
    12181 tcgcaaacca aaggctattc tagcgcaaat ggcgaagccg aggtagtgaa atcggaatca
    12241 gaaaaagaca aacgcctctt ggccaaagag cttcaacagg ctcaagaagc ggcaaagcag
    12301 gttggaggcc gggtagagtt acacgcagag aaagggctcg gcattgttgt cacgcttaaa
    12361 ctgcctttca ggctttttaa acacccgcat cagattaagg aaagtccgga aaccactcgc
    12421 aatggggaaa tttctggcgt cacgattctg gtggctgaag atgatccgat caatcgccga
    12481 atcgttagag ccctattaga gcgtcgtggt tggcgtgtca ttgaggttga agatggcgaa
    12541 caggccgtga atcagtggcg aactcggcaa cccgatttga tccttatgga tatccgtatg
    12601 ccgaaaatag acgggtacga agcgacgaag atgatccgcg aggaggagaa aaccagaggc
    12661 gcgggaacgg tcacgcctat cgtagcgtta accgcctacg cgatgcaaga agatagagag
    12721 aaatgcgtcg ctttggggat gaacgagtac gtttcaaaac caatacaaaa agaggaactc
    12781 atagccaaaa tacttcaatt aatcggccgc tccgaagcct gatacgaaca ggtcacctct
    12841 tccttatcaa accctcggga tggctcgttc ttcgaggcta tggttgaaac gatctggagg
    12901 tgtctgtatg caaagcaaaa aaccggtggt attgggcttg gttgcggccg tgctgcttac
    12961 cagcgccgtc tttggcgcga atccatctaa cctgtacgga cgaggggcga gggccacgaa
    13021 cggcgttgtc gctgccgcca agccggaggc ttcgcaggtc ggggttgaca ttttgaaaaa
    13081 aggcggaaac gccgtagacg cagccatcgc taccgccttc gctttagggg tattggaacc
    13141 gaacgcatcc ggtcttgggg gtggcggttt catgatcatc aaattagttg atatggctga
    13201 accggtgatc atcgattttc gagaatgcgc tccgttgagg gcgactcccg atatgtacaa
    13261 attcaacgcg cgaaaccagg tgatcgggaa cgaaaatgcg ataggcggta aggcttcggg
    13321 aatacccggt gaagtagcgg ggctgctttt cgccttggag cgctatggga cgatgacacg
    13381 agctgaagtg atcgccccgg ccatcgaatg ggcggaaaag gggatccccg tctccgccaa
    13441 tttgcggcaa attatgatgg acaactatat gaaactttta gagttcgacg ccacggcgaa
    13501 gatatacctc aacgacgatc tcccttacga ggtcggcgag accctatacc tcaaagacct
    13561 ggcgcgtacc ttgcgaaaaa tcgcccagga gggtgcctcg gcgttctaca cgggggaaat
    13621 cgcccgtcaa atcgtcgaag aagtcggcaa gcgaggtggc ctatttactc tcgaggacct
    13681 tgcgagctat gaggtcaagg tgagaactcc cgtgaaggga acctatcgcg gatatcagat
    13741 cctctcaacc ccaccggtca gttctggtgg cgcccatctg atcgaactcc tcaacattct
    13801 cgagaattac gacttgtctg caatcggcgc taatactcct ctctgggcgc atctgtggtc
    13861 ggaagcgatg aagctggtct tcgccgattt gtctaagtat atggccgaca ccgacttcat
    13921 cacggtaccg ctggaaggga taacctccaa agcctacgcg cgtgagcgag ccaaagaaat
    13981 cagcttgacc gggccgatgc ctaccgtcat agcgggagat gcctgggcta accaaacagg
    14041 tagcacgacc catctgtccg tagcggacaa agaaggcaat atggtcgcga tcaccaagtc
    14101 gatcaattct ttcttcggtt ccggcgttgt cattcctggg accggcattt taatgaacaa
    14161 tcatatggac gacttcgacc tgagaccggg ttcggcgaat tctatcgaac cgaaaaaacg
    14221 cccgctcagc agtatgtgcc cgacgctcgt gatgacaccc gatggccgcc cgtttatggc
    14281 gatcggttca cccggcgcct tccgtatcat caccaccatc gcccaagtga tcagcggcgt
    14341 gatcgaccac ggtatggaca ttaacaccgc catcttagct cctcggctgt tccggaccca
    14401 ctccgggccg atgtcggtgg aaggtcgtat ctctttagcc acttatgaag ccctgcgcaa
    14461 aatgggacac gaattggacg tccgcgggga tttcgataat ttcttcggtg gcgtacaagc
    14521 ggttctgtac ttacacgatc agaacgtttt agtcgggggg gccgatccaa gaagggatgg
    14581 acaagcgata ggctattaac cttaagatcg cggggtaacc ttcaaggcgt caaaaaaaac
    14641 cggtcgatca ccgggggaat gttcgtgctc catagacgat tccttctgtc ctgcctgcga
    14701 gcgtgcttgt agtacgcgcg cattacaaaa aagcaagcgc aacacgctat gagaccgttt
    14761 ttcgaaaaga aagaataaaa cgggtccaag gaaattcggt acgccatatc gcgttgttaa
    14821 gaggtcgacg aatgaccacg ggagggggca agtacctgca ccccgtatct acggagacgc
    14881 ccactctgtg ccgatccctc aggttccgaa cgcgtatata agcattttgg gattcgaaat
    14941 ttgatacttt accgcggctt ttggtatcac tgtgacacgc acgcagagga tgggttctta
    15001 aaaaaagcgg acgataatcc gtccgccaag agaagaaacg ttgtttctag ggatttttat
    15061 gcttgcacgg atgaacgcat ccgggaaagc tcaagtttca gactatctac cagttttatg
    15121 gtggaggcgc aacgcttgtc gtagttttcc tgtgtcttgg cgatggcgta atggactgtg
    15181 gaatgagttt tttcgaacca tttggacaat tccaacacgg ggatgcgcat ttgtttatgg
    15241 gcgacgtaga tcgcgacctt tctagcttcg gacacctctt ttttccgtga atcggaggtg
    15301 agatcggcct tagtgaagcc gaaagccttt tccacgatag aaaaataccg ctcgcgctcg
    15361 cttaaacgga tgatctgggc gtccgcgccg gaaagggcgg cccgatcctc ttctagaacc
    15421 tccttcaagg tgtgtatatc gatgctcttc ccttgtagtt ggttcatcat gatcagtttg
    15481 acgatcgtgc catagacctt ccgtatgttg tctttgatcg cgtgggccag gtaagagacg
    15541 accgaaggag acatcgtgat cttttcgcgt tttgatatct tctcgatgat gcgtttgcgg
    15601 gtttctatat ccggcgcgtt caattcgacg accaaaccca tctggaagcg agatatcaac
    15661 ctttcgtgga aggtgcctag gtctttcggc gttcggtcgc tgcagaaggc gatctgtttg
    15721 ttcgcatcga acaagagatt gaaggtgtga aacagttcgt tttggatccc cattttaccg
    15781 atcaaaaact gtatgtcgtc gatgaggaga atgtcgatcg agtgcctgta cttattccgg
    15841 aactgttcta ttctgtcttg tttgatcgat tggacgagct cgttcataaa ctgctcggaa
    15901 ctcaggtaca tcactttttt ttccggaaac tgcgaacgga cttcgtgtcc gatggcttga
    15961 agcaaatggg ttttgcccag cccaacgtct ccgaagatga atagcggatt ataccgacca
    16021 ggcgctgccg agacggattt cgcgctctgg aaggccaaaa ggttcccgcc tcccgctatg
    16081 aagtcttcga aggtgtattg attgttgaga gagcagattt ccagcggctt ttttccggtg
    16141 aatcgggtgg gagtcgattt cacttccccg ttttccggtt catcggtgag tagcgcgtct
    16201 ctaagctcaa aagagaatgc cgggccaaag aaggatttga cggcgctgga caggaccttg
    16261 ccatagcgtg taaggatcca atccttgacg aaaaggttgg cggtctgtaa taccacgagg
    16321 tttttatctg tcaagatctc caaaagctct agcgatttga accacaaatc ccagtgtttt
    16381 ttatcgacgt tagaacggag ataggcgaaa aaatcagtcg gtgcggattc gatctgtgtg
    16441 ttcctatttt gattctccat aagaaccccc gatgaggaaa agagtggagg tgttgaccct
    16501 accggactat cataccaatc gaggggcgtg agaacaaggg tctatttgta aaagacgggg
    16561 gtaaaaaatc ccaaaacagg aatttttact ccttcctctt gacatcctac gcaaaaaatc
    16621 gatataatca agcgaaattt ttgggaaacc tgaaggagga cgcgaaatat gaaaaggaca
    16681 tatcaacctt ccagagcaaa aaggaagaga acgcacgggt tcttggtccg tatgcgtacc
    16741 actaatggtc gaaaggtgat cgcaaaccgc agacgatccg gtaggaagcg cctggccgtt
    16801 tgatcccggg cagtttttga agaaacacgc ctttccaaaa acgcagcgcc tatcgaaaga
    16861 ctccgagttc gttcaggttc ttcgccgcgg gaagtcgatc gaaaccccct attttgttct
    16921 tctgtatttg gaaaaccctt atggaaacga cagaataggg atttcggtca agaaaaagtt
    16981 tggtaacgcg gttaagcgaa atcgtttgaa acgatgggtc agagaggttt ttcggacacg
    17041 cggaagaaga ggaaaggaga ctggcggcgc gcattttgac atgatcatac taccgaggaa
    17101 gaagttgtcc tcgagattcg aggagatgac gtttggggaa attgccaaac cgatattgga
    17161 gtgttttgag aaggttgata ggattgcccg cgcaagggat gacgcagctc atccgattct
    17221 atcagaataa catttctccg ttaaaacctt catgttgtcg tttttacccc acttgctcgc
    17281 attacgcctt ggcagtttac gagcgtttcg gcttcctgag agggtcgtat cttgctttga
    17341 agcggctgtc gaaatgtcat ccgctccatt cttttggcgt ggatttacca ccggagcgcc
    17401 tatctctttt tgagaaaaaa agataatacg gtatgcaaat gattctccca ggaggaaaca
    17461 ggatgaaacg tgcttttgca atcgccatct tattctttgt ctttgccttg accgcctttt
    17521 ccaaaaatga gttttcgctc gtctacgtgg acgggtttct acggatacaa acccggatct
    17581 cgcagcttac cttcaatacc cgggaagggg ctttgaacga gatggcgctg acgatggagt
    17641 cgtacaacaa aatatacggg tatggcgagg atggattcga cctttttctg aacgggaaga
    17701 agatcacgcc ggacggagtg tccatcaatg ggcgagatgc ggcttcgttc gcggaaagaa
    17761 ccgtgagctt ggaagaaaac gaaggcgtgg ttatcgattt ccattacggc aatatgatca
    17821 agcgcgttac cgtgccctac ggcccgtact atgaaatgtc catagagctt ctcggggaga
    17881 tccccgaggg gctgaccctt tcgctgccaa gacttgcgga ctttaatccg aacgataaca
    17941 cggaccgggt caacgaaacc ggaaaggtgt tcacctctta ttatcaaaga accgtcgcgc
    18001 tcgcggtatg ggcgatagag agagggcgca cagccttccg tctcgatggg tcgaagcacg
    18061 ccaacgagct cccgatcgcc tacgacggtg taaaaatcaa gggatacgtc gggccggcaa
    18121 agaagatgac tttcctccag acggtctttc cggaggagta tgtttggttg tcgcgaatcg
    18181 tgaacagcta tccgaaagcc tcttcttggt acgatccgct tttctatctg ttggtttatc
    18241 ttatggactg gttgtactac ctcacaaaga attacggctg ggctctgatc ctttacacca
    18301 tcattgtccg gatggcgctc ttcccactcg cttactccca gacgaaatct atgatcacgc
    18361 ggaagaagct cgaaaaaaac gcggaataca tcaagatcat gaaaatacag gacaatcaaa
    18421 agaagcagaa agagctgatg gatttttaca aaaagaacaa ggccagtccc gccgctggtt
    18481 gtctgcccgc cctcttgcag ctcccgattt ttttactact ctacgccgtt atccgttacg
    18541 aaagcgagct cttcgcgttc ggccccacgt ttttcttttg gagagaccta tcgataggcg
    18601 ggtttaaaga aaatatcttg atcgtgataa tttccttcgt gatcaatttt ttcaacaccc
    18661 tcatcaccag ctcggatacc aaacaggcga aacagggctt gctgatggcg ggcgtttttc
    18721 ccttcctgtt cattacgctg gccaccggtt tgcagatata ctgggtcacg cagagcgtta
    18781 tccagtgggc catcacgttc tggatgttca agagaagcga cactcaaggc attacgataa
    18841 gggagttttt actcagcttc aaaaagtgac aggtgataga gttgaaagag ttcgtggtat
    18901 cggcaaagac tgtagaagaa gcggtggact ctgtcaagag agagaatgga tttcgagaag
    18961 gagaatttga gtatcgagta gaagaccctg gctccagagg gtttctgaaa atagggtcga
    19021 ggaacgcttc gatctcggtt atcgtccgaa ccgaatacta cgtccggaag gtcaaagagt
    19081 ttatagagac cgtcctatcc ttctccaagt atgcgccgca gtcgttggcg gttaaggccg
    19141 aacaccggga tgcgaaaatc ttcgtccatc tcgacggggc gcatttgggg cggatgatcg
    19201 gaaaacacgg taaaacgatc agcgccctgc agcatctggc gaccatcttt atcaatcgga
    19261 tcaccgacac gaaaatgacg gtgttgatcg acgtcggcga ctacaaggac agacggaaag
    19321 agctgataaa aaggatcgcg cgaaataaag ccctcacggt gatacaaacg ggcaaaagcg
    19381 tgcaactgga tccgatgttt gcctttgaac gacgtatcgt ccacgagacg atcaagtcga
    19441 tatctggggt gcagagtttt tcaaaaggcc tggaaccgta tcgttacgtc gtgatagaac
    19501 ccgaaaagaa agggagacgg aagcatagga cgtcaccaaa aggaggtgtc atagactttg
    19561 aagatcatcg ttcgcaagcc ggatgaggtt cccgtcgtgc ggtatgacaa cccggaaaaa
    19621 gcgtggaaag tcaccaaaag agtgatggtc ggagaaaaag agggagcgcg tcatttcgtg
    19681 acgcgggttt tcaccatcga acccggcgga tacaccccga aacacaccca tccttgggag
    19741 cacgaaatct tggtcataaa aggaaaggga acggtattta ccgaggaagg cgaccgagaa
    19801 gtggagccgg gtaccgcgtt gtttgtccct cccgggtccg tgcatcaact ctccaaccgt
    19861 tcggacgaga acttcgtctt cgcctgcgtg gttcctatgg aaggagaaac ctagcccccg
    19921 cgctctagaa caaggggtcc gttttcatgg tagaatacct caaaatttat atctattaga
    19981 agaggtggtt gtgttgacca agaatgaagt ctttggacag attaaaaaga ttatcgtcga
    20041 gaagtttaat ctcgaagaag aggagatcaa actgggctct tctttcgtgg aggatttagg
    20101 ggcggactcg ttggacctcg ttgatctggt gatggcctta gaagacgaat ttggcatcaa
    20161 aatcgaagaa aacgacaccg atacgttgaa atgtatagaa gacgtagtca atttcatcta
    20221 cgaaaagctg ggtggcgcgg ccgacgagga ggacgtcacc gaggatgtgg atgaggactc
    20281 cgatgaaaag gtcaatgtcg acgaattaga tgaataaacg cttaaggaga gggcggccgt
    20341 cccgatgcag cgacgacatc gttccagaaa aggcttttcg ttattgctgg aacttttgct
    20401 ggcagccgcc ctttcttttt tttgtttcac cctcctatgc agctggtttg aacgaaacgc
    20461 gcggatagag aacacccgta aacgtatacg tgaggcgcga gatacgttcc tctttcaata
    20521 cgccctcctc gaaaacgggt attcggcttc agaaaaagaa ccggtacgac ggtacgcctt
    20581 aggacaggag accgttatcg aaatatacga gatctcgctt ccagaactca accgatctat
    20641 agaatgcggg ataatcatcc aaaaagagtc aggtgaatga tggacacaac cccattggaa
    20701 gttctaaaag aatccatatt tcatcttttg cgggccggcc acgacctccc ggcaggcgat
    20761 cctgtgccct ttcggatagg cccctctccc gaacggaagt tcggccattt ttccaccaac
    20821 gtggcaatgg cctacgcgaa ggtattgaaa actcctcctc ggcagatcgc cctctcactg
    20881 gtcaaggggt tggaagccct tcccgatatc gagagcgtct caatagaagg tgccggtttc
    20941 atcaatatgc tcctcaaacc ctcgtatttt caagagaggg tcctccgaat cctacaagga
    21001 gaatacactg ccaacttttc gttattcccg ccggacccga ttcaaatcga gtttgtcagc
    21061 gcgaacccga ccgggccgct aacggttgcc tcagcgcgca
//
LOCUS       DAOPXN010000015        20769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2563_length_20769_cov_5.581587, whole genome shotgun sequence.
ACCESSION   DAOPXN010000015 DAOPXN010000000
VERSION     DAOPXN010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 20769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20769
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2563_length_20769_cov_5.581587"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKM49_01690"
     CDS             complement(<1..466)
                     /locus_tag="PKM49_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81559.1"
                     /translation="MFTVPEKIIYALPEQIGNTELFVGRKKEFDYFLGDWYDYLVNNM
                     AQSQAIVARRKKGKTAFLQRLFNILWSCPETGVIPFYYVIQDKPVTLSGFAEDFFTQF
                     MWQYLSFRDRKEEYLSYPIGFEKLAPLVEGDKHLTDAYETMAIKKKEDDWDGM"
     gene            1226..4678
                     /locus_tag="PKM49_01695"
     CDS             1226..4678
                     /locus_tag="PKM49_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81560.1"
                     /translation="MRKSKYYELAGFSDQEMEEIRPELSAYLKEEFLNRGSPIEDFKY
                     RGTLCAFLVHIGAKTYEEGNFWSSIFQALDIQTPSVALEQKIGELFLKTCRDGLGFYF
                     PHTGFRFVVAILAHSFVPDSYIPKWARYIRWAFPEIESDRPVSDEKIAEELRKIKESY
                     QPEVIAGREKTAQLRKSLEKSLAYWPLIMLYREAPEATSYYTEFLDNPMMMRVIEEEL
                     PNRWAEKARELSRYQLKKELVVFLADSPFVEEDRHRTRLVRLKDTYQRAIDRYVVLKE
                     AVSRINRVRNLLSPGKPISLDDLRAKLEVVREWKEEHAGPLSWDVVKALYHFIKNHYP
                     DALNLSETALFDFADSLFTDQEIPAEPPFGDSAEPPFGESGDATRNRFLCEGTRCFLS
                     QSGNDAHIFIRSTIEYIRALEGLDKALLDEADRWPLLSKRLRKGIRSEIEHRTHIVQT
                     PRQSAPYLELDLDLERLWLVIPPIVLNEIDEQEKIFLSVVEAKANREVFVRECSLFNQ
                     GGSIETQRERCEISPFFGTLEIRAFKVNRLMNSKEPIREFSIKPQLCEEYPLLVFDEN
                     GEALKKREYATDYLYLILSGENEITPAEAVSDRTRWYGAPNFDYAALDLSDTDCFQIR
                     SPTGGSLAFYRAGFYGKLSESPVEGVTARGENVYFRCPTIRMPPATGDAGAWKMEVVL
                     ESGPSRVSTFQVANNPAESDSPSESMEVCPAEFFDSPYGRYRISIFGVKRLPIDMVFW
                     VLPRFRFRWSSEDRSLHAPGRLEIKAEDEIHFSFPALDPSEIQSEEGGMEIRCRIDPG
                     VQTVQAVLTLNPSGSDGLTFSCEFERPFVDEDTSESLSEYSPIFYGYPLKKEFRLPYD
                     LRGKQFECRHDQTGQITRCSVKKDRLSVDMRPFFDNIRASETAEQSFSIYERGLRYAE
                     TPPIATFSFRVPFADAEVALQEDADAQVTHVHCRWSREDNPSGLRLRLSTEGDKHFEA
                     FLDAGRSEYSFKVQTRAFPSGPFTVEWVVGNRVAREFHGLHEPDVSLAFTKDDIFAKD
                     AGLEILKVTDEQDREYALAGPKCVIRNIRPTDKIEEGEPYEGDIVWLDDKGYRKYWLN
                     TNPVRFFINEDHQTLPFLIDKEGDGAYYDVDEKLLYWIEKHRREDILVERVYFAFRRL
                     TRAD"
     gene            4668..9464
                     /locus_tag="PKM49_01700"
     CDS             4668..9464
                     /locus_tag="PKM49_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY81561.1"
                     /translation="MPINPIRMTEDVLEKYRSYLLTTFHLSDPELHGKFQEEIEKENA
                     LCKGPYIERLSRYRTGKSIGELVREGILSEIFLRIDQETLPSDRPLYAHQEMAVRKAV
                     VQKKSFIVSAGTGSGKTESFLIPVLNYLFRQIEESKLTPGVRALLLYPMNALANDQLL
                     RLRSILKRIPEVTFGIYTGETEQEEKSALSRFVRLFAHDPLPNELISRARMRGSPPHL
                     LITNYAMLEYLLLRPDDCVFFDGEHARFWKFLILDEVHTYGGAKGIETALLLRRLAHR
                     IGRSAQGQALQYFGTSASLGYSPDACSKLAQFAQNLFHTRVEWDKNDPERQDIIVGEK
                     LPYTPRELQVKAGPITIPPEFYTDLIAYFKGEANSLVTEKLSRWIGETIGNRAHNPDG
                     SPTLKRAEGPNAPFDLSDTFLRIVENDEKVKEVNRILESGPCEFSKLTEILFPKDAQR
                     EVLSFHLVLLINLVCHLFPQTSLSSMKYHLFLKALEGAFVSFYPDKTLQLERKTIEKM
                     DDSSTERLFFEIGSCKNCGAVYLTGTVDKDTGILRQTEYDQTEGDEFLDWYLLIEPDR
                     AVAENEDEEDGASEERDTRNLFQICPRCGKVSQENLIGNACDCGVALLSAKKAKSKEG
                     RVNKCLNCGAQSSLTPVLWRFLDGADASGSVIATALFQNLYSGLSPEERKRQNGFIVF
                     SDSRQGAAYFAPYLQESYDRIFWRRILVNTLETYREKAIENDWIVKNLAEFAKKRLPE
                     DYQMDKQTIETTAYLWTQYEAMSYDRRTGLEGAGLLGFKVPLSKGLYESLEKRSPLLN
                     APWNLTPEEAHSLYQLLLSSFRKCGALTYLPDIEPEHEFFAPRNLKTLMTLSAQTQAG
                     VKSWVPYRFENARSDYLRRLFPKARKEEVPELLQWIWKEFDHPDWRDCFETSFTKRNQ
                     SGKRLKIDALEILSPLLTPDMKLYQCDRCKELFFFAVRGVCPSYGCQGTVRETNFSAL
                     GPNHYRDLYRTLELSPLRVEEHTAQLKYEKAREFQEDFYHKKIQILSCSTTFELGVDV
                     GDLDTIFLKNVPPTASNYLQRAGRAGRRTSNPAFILTYALRRPHDRYFFSDPEKMIKG
                     EIAPPSFVLENEKIRYRHMAAIALAEYWRQYPNHFKDVKAFFFPETDDDLEGVARFEH
                     FVATHQEELDKTIRETIPATEHELRQKQFLPWAYLLFSDSPVTHASLYHIGKELKEDI
                     RVLTDLIDAATKKRTFRKADTLHRKVQTLLGRNLMQYLAGKGILPRYGFPVDVIELMA
                     PFDEKMDDLELSRDMKTALWEYAPDNPVYANKQKWSTQYIKKVPDKALIEESYAFCRK
                     CGFSKVSIKERSTTKTCKICGSQLNVGDYLFPEFGFISGQPEKAGVIKKTQLSQTRCL
                     LGKEEYTIEEKTLLLRYPVHLKTMLGNLVTINPGGPRGFNVCLACGYSDAKKTLEREH
                     NNPQGKRCPGKIARRKALGYQFKTEILEITPPENKRTNDEKEYWLSILYALLEGVSLF
                     LDIDRNDLDGIVFPVEGRSYPNLLLFDNVAGGAGHIRWCMEDGAFEGILKLTESFLKG
                     CLCGGEEGDSSCYGCLRNYTNQRYHEILKRKYAVEWIEEMLEGGRE"
     gene            9461..>10468
                     /locus_tag="PKM49_01705"
     CDS             9461..>10468
                     /locus_tag="PKM49_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81562.1"
                     /translation="MNDIDGNEERRLEEGFSLGNADNELVAVLNEFTLSVRELNAKYA
                     ELLENTRRIHTESGELHAALQKATEGAAEQRERSCANIAELADLAKELADLISSTHTE
                     LLENSRKISEESGGIQTAIRQAAEEATDQREHDLAKLAELSGLVKDLAESAGVAQLEL
                     LENTRKIYEESGGIQTAIRQLAEGAAYQREHDRAEIAGLSGLVKGFAESFGATQAEML
                     ENARRGNTESEKLHTAIREATEEAAKQREHSCANLAELSDLAKELADLISSTHTELLE
                     NSRRINEESGVIRTAIRKVSEEVASQWEQNRTEVAELAGLVKELAASTSSRQTESLEK
                     TS"
     assembly_gap    10469..10568
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10569..11598
                     /locus_tag="PKM49_01710"
     CDS             <10569..11598
                     /locus_tag="PKM49_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81563.1"
                     /translation="ELAASTSSRQTESLEKTSKISEESGEIQLAIRQAAEETASQREH
                     DLAKLTELSGLVKDLAESAGVAQLELLENTRKIYEESGGIQTAIRQLAEDATNQRERG
                     RAEIAGLSSLTKELADLISSTYTELLENTSKINTESGGIQTAIRQLAEEAANQREHDR
                     AEHAEFSDLVKRLADSTGDLGARLRSLVDEKTGLSRLDPAFVSTVDRKLGNLIASFGE
                     GNRETLDALREMDENVHASLQDSRNLAVLTQKTVEALPKLLKGHYSESIATLTEKTAA
                     FESALTALDELEKKTAEKQQAFDDRLTALEKRVKEKMDRTYFFQLMVFASQWVALIVF
                     MLFSFWKV"
     gene            11651..12268
                     /locus_tag="PKM49_01715"
     CDS             11651..12268
                     /locus_tag="PKM49_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81564.1"
                     /translation="MTQIYAPGMRIQVRGEEWVVRRADYNADRKYILSVTGVSDFVRG
                     QEAAFLSDLEEIRVIDPADTQFVVDRSPMFRKSRLYIESLLRRRTPPGNVLRIGHKAA
                     MNVIDFQLEPAQRALARPRQRILLADGVGLGKTLEAGVLMSELIARGKGKRILVVALK
                     SMMLQLQKELWTGSPFRSNGWTPEGSNVFAPTFRRTITHFTISIR"
     gene            12265..14478
                     /locus_tag="PKM49_01720"
     CDS             12265..14478
                     /locus_tag="PKM49_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNY81565.1"
                     /translation="MIISIDTLKMDREYRTLLEKTRWDIIVIDEAHNVARRGKTQAQR
                     SRLAELLSKRSDTLILLSATPHDGRPQSFASLMNMLEPTAIANEEDYRREDIQGMVFR
                     RFKKDVQNQVSAHFKERVISRETCEASPLEEAFYDFFETLNLTWIDPARGETMLFKTL
                     LEKSLFSSPVACEKTAQARINRLKKEGRSESASDIRALETLIDHLKKITPEHFSRYRL
                     LVRLLQSPEYGWNRERPDDRLVIFTERIETMRFLAKRLKEDLRLSDGQLSCLYGGMSD
                     VDQQQAVDEFGATDSPVRLLIASDVASEGINLHFLCHRLIHFDIPWSLMVFQQRNGRI
                     DRYGQEKTPDIRYLVTKAKNERIRGDMRILEILIDKEEQAEKNIGDPASLLGRYDVEE
                     EERTVARVFEKSLSVNAFERLLSAPENPFLAMMNAGWNLPDEAAKATNVEKADPVTLF
                     DETSYLKTLLETISNRETIPLEAPRVDVMEIRMSESMKRSLEPQIPEEAFPSDDWLRL
                     TTDKKALMREIKESRQKAQDTWPRSHYLWPLHPIIDWMNDKAGLFFGRQEAPVLFVPF
                     AEKRTAVFIISGLIPNRQSEPVIDRWFVIRFVEDNPAEFLSPEDFLRSDVFKGALPNQ
                     ASRNAEVSDWAAKLLPTAIGLAKEKMKAEQEEFEETMTPKLDEEIAKLKELRKKHDDL
                     LQSYDRSDGRNAQRYEIEKRRIDEIFNEFQHWLVNTMKLEKDNPYIRVVALLLSE"
     gene            14488..19176
                     /locus_tag="PKM49_01725"
     CDS             14488..19176
                     /locus_tag="PKM49_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81566.1"
                     /translation="MAIDLSGIHNVNEYYTAHYLSTIFEENIREALSMWKAQAQESEE
                     KEPWRKIKSLGRAYFNRLDEYTRERDEHRRREIRRSFLKELFSALGFLFSEADEEFIG
                     NDGITVPVWQILRKPDGAPYLLLIETDETDNGSALESVAEAFPDCDYEEALNRYIFNA
                     PEPPRWVILCSMREIALLDRYKWNEKRFFQFILDEIYARREDTTFQAMAVLLDKSSLA
                     PFDGEPLLDRFDDTSHKHAISVSEDLKYALRRCIEWLGNDAVRDMKERQKVKVYDTDL
                     AEQLTLECLRFMYRILFILFIESRKELEYAPVQSDVYMKGYSFESLRDLVDKVRLEGE
                     ESRNGYFLDTSLKKLFSLIYEGYPKRNGTVKTARAEALGLEGVGADAFEIPPLQSHLF
                     DPERTPLLNRARFRNETLRDVIREMSITRPRGRERAGRVSYSHLGINQLGAVYEGLLS
                     YRGFFAEEDLYEVQPKGESWDELQVGYFVPASRLEEYEETERVRNPDGSLKLYPKGTF
                     IYRLAGREREKSASYYTPETLTQCLVKYALKELLKDKTADEILELKICEPAMGSAAFI
                     NEAVTQLAEAYLQLKQRETGQTIAVDRYAEEKQKVKMRLSDRNVYGIDLNPIAVELAE
                     VSVWLNTIYKGGYVPWFRTQLMNGNSLIGARRQVYRPDQIREKKKEKQWHTVAPERVE
                     PKTTRPAGSVYHFLLADPAMATYEDKVVKKLKAEELKRFKEWNKDFSRPYDAEEIKTL
                     QRLSDQVDLLWEKHLKARKKVEELTESAISIFGHEEKARRRPYTTKEKDAIYESLFHT
                     EKMENAGPYARLKLAMDYWCALWFWPIDKADLLPSRSEFLMHLSLILEVNLIPTAPAQ
                     RPLFVENDAEKQLQTLFSDLDLVDLDALCEKLEPLRVVREVARRIAFFHWELEFADVF
                     DERGGFDLILGNPPWIKLGWEEGGILSDAEPLFAIRKYSADQVSKLRNDSFERQPELL
                     EDYVREYETMEATKGFLNAVQNYPLLQGSQSNLFKCFLPQSWLFANRNGVQGFLHPEG
                     VYDDAKGGALRRELYPRLRYHFQFQNQYMLFPIGHRVKYGINIYKSNFHEISFDSLSN
                     LFLPKTIDDSFDHQGDEPLEGIKNEQDEWNLKGHHLRVVHVDEGALTVFSQLFDEEGT
                     PPLESKLPSVHAQPIVGVLKKFAAYPHRLRDLKGEYHSTVMWDETNAQKDGTIKRETI
                     FPKTAEELILSGPHFHVGTPFYKTPRAICTEKGHYDPLDLAELPDDYLPRTNYVPAPD
                     RMLYQNRIPTAKWSLNQGNRSDLVTDYFRMIHRRRLSQFGERTFISALIPKNIAHVHT
                     VISTFFLEPRRLLQAFVPACSLLSDFFVKTSGKEDFTTGNVDDLFYLEDDSRFLYALP
                     RALTLNCLTTPYAELWEELFDPRFQKDTWTSEDPRLDRDFFRNLTPTWQRHNALRYDY
                     ARRQALVEIDVLTAMALGLTLEELISIYRIQFPVMRQYEKDTYYDRKGRIVWTNSKGL
                     TGVGLRERSEWEALQELKDGESCSRMVKDDTRPGGPFEREVRYFAPFTLADRETDYAV
                     AWREFERRGL"
     gene            19276..>20769
                     /locus_tag="PKM49_01730"
     CDS             19276..>20769
                     /locus_tag="PKM49_01730"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY81567.1"
                     /translation="MIPSIVCEQLQRGLTEYLKTTFPISSEVFAGCIDRFLDARSMFK
                     GPYVTLRMPFREAARENPWFEALELPFAPYLHQKRAFDRLAGEAPRSTLVATGTGSGK
                     TECFLYPLLEHCWRHRGEKGIKALLIYPMNALATDQARRIAGLIYRNAKLKGNVVAGK
                     YTGDSGPGASRGMGEQELITDREMLRQNPPDILLTNYKMLDYLLIRSDDFPLWSKNGP
                     ETLKYIVVDEFHTFDGAQGTDLACLLRRLKKRLEIPEGFLCCVGTSATLGDAQSQTEM
                     VAFASEVFGEPFPEDAVIGEQRQTADEFLRGTEIQYTALPDKKALSEIANAMTSGSER
                     DFIRLASLRWFGDATLGDDDNDARGRELMGERLRGHYFFHELLRALEGGASSYEELLA
                     ELSRRNRILDFYSKPDRHLLLLSLIGLVSYARILQVHVQIWFRELRRLLGKVDPEKPD
                     LALSDDLTEGERGQYLPVVNCRDCGVTGWVSMMSDSFNTKIIDLRGFY"
BASE COUNT         5176 a         5601 c         5621 g         4271 t
ORIGIN      
        1 acatcccatc ccaatcgtct tctttctttt ttatcgccat cgtttcgtac gcatctgtta
       61 aatgcttatc gccttctacg agtggggcta acttttcaaa gccgatcggg taagatagat
      121 actcttcctt cctatctcta aaggaaaggt attgccacat aaattgggta aagaaatcct
      181 ccgcgaaccc agacaacgtg acaggtttat cttgtatcac ataatagaac gggatgacac
      241 ctgtttccgg gcagctccac aggatattga acagtcgctg cagaaacgcc gtcttccctt
      301 tctttcgcct ggcgacgatc gcttggcttt gagccatatt attgaccagg taatcgtacc
      361 aatctcccag aaagtagtcg aattccttct ttctcccaac gaaaagctcg gtgtttccta
      421 tttgctccgg taaggcgtag attatctttt ccggcaccgt gaacattgct ttcacccgct
      481 ttgtttgtgt ttggaaagat tatagcacag aacctgcgaa gcacttggga acgctcgatt
      541 tcagggaagc cacatttgca gcgcctgaga actccctggg gtggcgccga ctccccagcc
      601 ggcgtacttt tttgcgtcgc actgctggga acgccaactt ttaagttggc atgttttagg
      661 tttttgggaa gtcatcgcgg tggaaagaaa acaaggggtt atcgcggggt cccgttggtt
      721 accccgcgat aacccgtttt gttatgtcac ctcattgtgt gcccccacag cgtcccgggg
      781 tggcgccgac ttcccagtcg gcaggggttc tgggaacgcc aacttttaag tcggcaggtt
      841 tttcctgtgg atgtctgaca aaacttaaaa ctatgccaaa ctaaagtttg gcgttcccag
      901 gcgttcccaa gcgtatcgcc ccgtcgcaaa atgatgaaac gcttacgccg cgatgaccac
      961 ctctcgaccc tacgtttggt atgttttcta tcttttcttt aaaaggaaca cctggctttt
     1021 ttgcgtgtct tccctcaaac gcacccccgt accactacaa atttcctaac tttggaaaac
     1081 cccgctcaat gtgacgaatg gtttatcgtt cctttcttta ctcgaataaa tgatgtataa
     1141 tcgatgtaga tcgcgatcgc ctcggaaagg aatgataccg ttgagcgcga agcttaccct
     1201 ttttgatttt caaatgtata aggcaatgag aaaaagtaag tattacgagc tcgcaggctt
     1261 ctcggaccaa gaaatggaag aaattcgccc ggaattgagc gcttacttga aagaggaatt
     1321 cctgaatcgg ggatcgccta tcgaagattt caaataccgg ggaaccctct gcgcctttct
     1381 cgttcatatt ggggccaaaa cctatgaaga agggaacttt tggagttcga tttttcaagc
     1441 gctggacatc caaacaccct ccgtagcttt ggaacaaaaa atcggagaat tgttcctaaa
     1501 aacctgtcga gatgggctgg gcttttattt ccctcatacc ggctttcggt tcgtcgtggc
     1561 catcctcgct cacagtttcg ttcccgactc atacattccg aaatgggcgc gctatatccg
     1621 ctgggctttt cctgagatcg aaagtgatcg accggtgagc gacgagaaga tcgcggaaga
     1681 actccggaaa atcaaagaat cctaccagcc tgaagtaatc gcgggtagag agaaaaccgc
     1741 ccagttacgt aagtctctgg aaaaatcatt ggcttactgg cctttaatta tgttgtacag
     1801 agaggcgccg gaggcgacct cctattacac cgaatttctt gataacccta tgatgatgcg
     1861 cgtgatcgaa gaggaattgc cgaatcgctg ggcggaaaaa gcgcgcgagc tctcccggta
     1921 ccagttgaag aaagaattgg tcgtcttttt agcggactcg ccgttcgtcg aagaagacag
     1981 acatcggacg cgcctcgttc gattgaaaga cacctatcaa agagcgatcg accgatatgt
     2041 cgtattgaaa gaagccgtca gccggatcaa ccgcgtgcga aaccttctct ctccggggaa
     2101 gccgatttct ttggatgacc ttcgcgcgaa gctcgaggtg gtacgcgaat ggaaggaaga
     2161 gcacgcggga cctctcagtt gggatgtcgt gaaggccctg taccatttca taaaaaacca
     2221 ctaccccgat gctttgaacc tctcagaaac ggcgctcttc gatttcgcgg attctctatt
     2281 cacagaccaa gagattcccg cagaacctcc ttttggagat tcagcagaac ctcctttcgg
     2341 agaatctggg gatgctacaa ggaataggtt cttgtgcgag ggaacacggt gtttcctctc
     2401 ccaatccgga aacgatgcgc atatttttat aaggtcgacc atcgagtata tccgcgcgct
     2461 cgaaggcctc gacaaggcgc ttctcgatga agctgaccgg tggcccttac tctcaaaaag
     2521 gctgagaaaa ggcataagaa gcgaaataga acatcggact cacatagttc agacacccag
     2581 gcaaagcgct ccctaccttg agttggacct cgatttggag cggctgtggc tcgttatccc
     2641 cccaatcgtt ctcaatgaga ttgatgaaca agaaaaaatc tttctgtccg tcgtggaagc
     2701 gaaagcaaac cgagaggtct ttgtcaggga atgttcgctt tttaatcagg gcggctcaat
     2761 agaaacccag agggaacggt gcgaaatttc accgtttttc ggtacactag aaatccgcgc
     2821 ctttaaagtg aacagattga tgaacagcaa agagcccatc cgcgaatttt ccataaaacc
     2881 acagctctgt gaggagtacc cgttactggt cttcgacgag aacggggagg cgttgaagaa
     2941 gcgtgagtat gcgaccgact acctctacct cattctgtcc ggcgagaacg aaatcacacc
     3001 ggccgaagcg gtctcagaca gaacccgatg gtatggagcc ccgaattttg actacgccgc
     3061 tctcgatctg tcggatacgg actgtttcca aatcagaagc ccgaccggtg gaagtctcgc
     3121 tttttatcgg gccggtttct atggaaagct cagcgaaagt cctgtcgaag gggtcacggc
     3181 gcgtggagag aacgtctatt tccgttgccc gacgatacgg atgcctccgg ccaccggcga
     3241 tgcgggcgcc tggaaaatgg aggttgttct cgaatcaggc ccttcacgcg tttcaacctt
     3301 tcaagtcgcc aacaaccccg cggaaagcga tagcccgagc gagtctatgg aggtatgccc
     3361 cgcggaattt ttcgattccc cttatggtcg gtatcgcatc tctatcttcg gggtcaaaag
     3421 gcttccgatc gatatggtct tctgggtgct gccgcggttt agattccgct ggtcttcgga
     3481 ggataggtcc ctccacgcgc cggggcgctt ggaaataaag gcggaggacg aaatccattt
     3541 ttcttttccc gcgctcgatc cttccgaaat ccagtcggaa gaggggggca tggagatccg
     3601 ttgtcgtata gaccccgggg ttcaaacggt tcaagccgtt ctgacgctca acccctccgg
     3661 ctcggatgga ttaacgttct cctgcgaatt cgaaaggccc ttcgtcgatg aagacacctc
     3721 agaatcccta tccgagtact ccccgatctt ctacggctat ccccttaaaa aagagttccg
     3781 actcccgtac gacttgaggg gaaaacagtt cgaatgccga cacgatcaaa cggggcaaat
     3841 cactcgttgc tccgtgaaga aggatcgatt aagcgtcgac atgcggcctt ttttcgacaa
     3901 catacgcgcc agcgagaccg ccgagcaatc tttttctatc tacgaacggg ggcttcgata
     3961 cgccgagaca ccaccgatcg caaccttttc ctttcgcgtc cccttcgcgg atgccgaagt
     4021 cgcgctccaa gaagatgccg acgcgcaggt gactcacgtc cattgccgct ggtctcgtga
     4081 agacaacccc tccggactgc gcttgcgcct ttccactgag ggtgataaac acttcgaagc
     4141 ctttcttgac gctgggcgga gcgagtacag ttttaaggtt caaacgcgcg catttccttc
     4201 cggccctttc accgtcgaat gggttgttgg taaccgggtc gcgcgggaat ttcacggtct
     4261 ccacgaaccc gatgtttcac ttgccttcac aaaagatgat atatttgcaa aggatgcggg
     4321 cttggagatc ctcaaggtca ccgatgagca agaccgagaa tacgccctgg ccggaccaaa
     4381 atgcgtcatc cggaatatca ggcccacgga taagattgag gagggagagc cctacgaagg
     4441 cgatatcgtc tggttggatg acaagggtta tagaaaatat tggctgaata cgaacccggt
     4501 ccgctttttc attaacgaag atcatcaaac ccttccgttt ctcatagaca aagaagggga
     4561 cggcgcctat tatgacgtgg acgagaaact cctctactgg atagaaaaac atcgccgcga
     4621 agatatcctc gtagaaaggg tatacttcgc attccggagg ttgacccgtg ccgattaatc
     4681 cgataagaat gaccgaagac gtcttggaaa agtaccgtag ttatctcctg acgaccttcc
     4741 acctctccga cccggaacta cacggtaaat tccaagaaga aatcgaaaaa gaaaacgcgc
     4801 tatgcaaagg gccctacatc gaacgtttgt cccgctatag aactgggaaa tcgatcgggg
     4861 agctcgtgcg ggagggaatc ctctccgaga tcttccttag gatcgaccaa gaaacgctgc
     4921 cttccgatcg accgttatac gcgcatcaag aaatggcggt gagaaaggcg gttgtgcaga
     4981 aaaaaagctt catcgtctcc gccggtacgg gaagcgggaa aacggaatcc ttcctgatcc
     5041 cggttttgaa ttaccttttc aggcagatcg aagaatccaa gttgacgccc ggagtcagag
     5101 cccttttact gtatccgatg aacgcgctgg caaacgatca attacttcgt ttgcgcagta
     5161 ttctgaaaag gataccggaa gtgaccttcg gcatctatac cggggaaacc gaacaagagg
     5221 aaaagagcgc gctgagccga ttcgtgaggc ttttcgcgca cgacccctta cccaacgagc
     5281 tcatcagtag agctcggatg cgtggctcgc cgccgcacct gttaatcacc aactacgcga
     5341 tgctcgaata cctgctcctg agaccggacg attgcgtttt cttcgacgga gaacacgcgc
     5401 gtttttggaa gttcctcata ttggacgaag tgcacaccta cggcggcgcg aaagggatcg
     5461 aaaccgcgtt gctgctgcgg cgtctcgctc accgtatcgg gcgaagcgcg caaggccaag
     5521 ccctgcaata cttcggcacg agcgcctctc tcggctattc cccggacgcg tgttcgaagc
     5581 tcgcgcaatt cgcacaaaac ctttttcaca ctcgcgtcga atgggacaaa aacgatccgg
     5641 agcgtcagga tatcatcgtc ggcgaaaaat tgccctacac gccgagggag ctccaagtca
     5701 aagccgggcc aatcactatc ccaccggagt tctataccga tctgatcgcc tattttaaag
     5761 gagaagccaa ctccttggtg actgagaaat tgagccggtg gataggggag accatcggaa
     5821 accgcgctca caatcccgac ggatcgccga cattgaaaag ggcggaagga ccgaatgcgc
     5881 ctttcgacct ctcggacacg tttctgagga ttgtcgaaaa cgacgagaag gtgaaagagg
     5941 tcaatcgaat attggaaagc ggaccttgtg agttttccaa actaacggaa atcctctttc
     6001 caaaagacgc gcaacgtgaa gtactgtcct tccatttggt cctactgata aacctcgtgt
     6061 gccacctctt tccgcaaacc tccttgtcct ctatgaagta ccatctattt ctcaaggcct
     6121 tggaaggcgc tttcgtctct ttctacccgg ataaaaccct ccaactcgaa cggaaaacca
     6181 tagagaagat ggacgactct tccacagagc ggttgttttt cgagatcggg tcctgtaaaa
     6241 actgcggggc ggtctatttg acagggaccg ttgacaagga cacggggatt ttgagacaaa
     6301 ccgaatacga tcaaaccgaa ggggacgaat ttctcgactg gtatctcctc atagaacccg
     6361 acagagcggt tgccgaaaat gaggatgaag aagatggggc atccgaagaa cgggatactc
     6421 gcaacctttt ccaaatctgt ccccgatgtg ggaaggtctc tcaagaaaac ttgatcggga
     6481 acgcctgcga ctgcggggtg gctcttcttt ccgcaaaaaa ggcaaaatcg aaggaaggac
     6541 gcgtgaacaa gtgcttgaat tgcggcgcgc aatcaagtct aacccccgtt ctttggcggt
     6601 ttttagacgg ggcggacgcc tcgggaagcg ttatcgccac ggccctcttt cagaacctgt
     6661 atagcggcct gtctcccgaa gaaaggaagc ggcaaaacgg ctttatcgtc ttttcggaca
     6721 gtcgccaagg cgcggcgtac ttcgcgccgt accttcaaga gagttatgac aggatcttct
     6781 ggaggcgcat tttggtgaac acgctcgaaa cctatcgaga aaaggccatc gagaacgatt
     6841 ggatcgtcaa aaacctggcg gaattcgcca aaaaacgcct cccggaagac taccaaatgg
     6901 ataagcagac aatagagacg accgcttacc tatggacaca atacgaagcg atgagttacg
     6961 atcggcgcac gggtctcgaa ggcgccgggc tcctgggctt caaggtccct ctttccaaag
     7021 gactctacga gtctctcgaa aagcgaagcc cgctcctgaa cgcgccttgg aatttgactc
     7081 cggaagaggc ccacagcctg tatcaactct tactttccag tttcagaaag tgcggcgctt
     7141 tgacctactt accggacata gaacccgaac acgagttttt cgcgccgagg aatttgaaaa
     7201 cgttgatgac gctgtcggcg caaacccaag caggcgtgaa aagttgggtc ccctatcgtt
     7261 tcgaaaacgc gcgctccgat tatttacgcc gcctatttcc aaaagcgaga aaggaggagg
     7321 tcccggagtt acttcaatgg atttggaaag aattcgacca tccggattgg agggactgtt
     7381 tcgaaacctc ttttacaaag cgcaaccaaa gcgggaagcg attgaagata gacgccctgg
     7441 aaatcctttc gccgctcctg actccggata tgaaacttta ccaatgtgat cggtgtaaag
     7501 aacttttctt ttttgcggta cggggggtct gtccttctta tggatgtcaa gggacggtga
     7561 gggagacgaa cttttcggcg ttaggcccta accactaccg agacttgtac cgcacgctcg
     7621 agctctcccc gcttcgagtg gaagagcaca cagcccagct taaatatgaa aaagcgcggg
     7681 agtttcaaga agacttctat cataaaaaaa tacagatcct cagctgctcg acgaccttcg
     7741 agttaggcgt ggacgtcggg gatttggaca cgatctttct caaaaacgtg cccccaaccg
     7801 cgtcgaacta cctgcaaagg gcagggcggg ccggcaggag aacctccaat ccggctttca
     7861 ttctcaccta cgcgttacga aggcctcacg accgttactt ctttagcgac ccggaaaaaa
     7921 tgattaaagg cgagattgcc ccgccttcct ttgtgctgga aaacgagaaa atacgctatc
     7981 gtcatatggc cgcgatcgca ttggccgagt attggcgtca gtatccgaac catttcaaag
     8041 acgtgaaggc cttctttttt ccggaaacgg acgatgacct cgaaggggtc gcgcgcttcg
     8101 aacacttcgt cgcgacgcat caagaggagc tggacaaaac gatacgagag accattcctg
     8161 ctaccgaaca cgagctgcgc caaaaacaat ttttgccctg ggcgtacctc ctgttttccg
     8221 attctccggt tacgcacgct tctctctacc acatcggcaa agaattgaaa gaagacatac
     8281 gggtcttgac ggacttgatc gatgcggcga ccaaaaagag aaccttcaga aaagcggaca
     8341 cgctccatag aaaggtgcaa acccttttag gccgtaatct catgcaatac ctggccggaa
     8401 aagggattct acctcgctac gggtttccgg tcgacgtcat agaattgatg gcgcccttcg
     8461 acgaaaagat ggacgacttg gagctctcgc gggatatgaa aaccgccctt tgggaatatg
     8521 cgccggacaa tcccgtctac gcgaacaagc agaagtggtc gacgcagtat atcaagaaag
     8581 tgcccgacaa ggcgttgatc gaggagagtt atgccttctg ccgaaaatgc gggttttcga
     8641 aggtgtcgat caaggagcgc tcgacaacca agacgtgcaa gatttgcggc agtcagttaa
     8701 acgttggcga ttaccttttc cccgagtttg ggttcatctc cggacagccc gagaaagcgg
     8761 gtgtgataaa gaagacacag ctctctcaaa cgcgctgcct gctcgggaaa gaagagtaca
     8821 ccatcgaaga aaaaaccctc ctgctgaggt atccggttca cctgaaaacg atgttgggca
     8881 atttggtgac catcaatccc ggcggcccgc gggggttcaa cgtttgtttg gcttgcggtt
     8941 actccgacgc caaaaagacg ctggaaagag agcacaataa ccctcaaggc aagaggtgcc
     9001 caggaaagat cgcccgtaga aaagcgctcg gctaccagtt taaaacggaa atattggaga
     9061 tcacccctcc ggaaaataaa cggacaaacg atgagaaaga atactggctt tctatcctct
     9121 acgcgctttt agagggggtt agcctgttcc tggacataga ccggaacgac ctcgatggaa
     9181 tcgtctttcc cgtggagggc aggtcgtatc cgaatttgct tctgttcgac aacgtcgcgg
     9241 ggggcgccgg tcacatacgc tggtgtatgg aagatggagc gttcgaggga atcctcaagc
     9301 ttaccgagtc ctttttaaag ggttgcctat gtgggggcga agagggggat tcctcttgtt
     9361 acggctgttt gcgtaactac accaaccaaa ggtatcatga gatactaaag aggaaatatg
     9421 cggtagaatg gatagaagaa atgctcgaag gagggcgcga atgaatgata tcgatggaaa
     9481 cgaagaaaga aggctagagg agggtttttc gctgggaaac gcggataacg agctcgttgc
     9541 cgttttgaac gagttcacgc tgtccgtccg agagctcaac gccaagtacg ccgaactatt
     9601 ggaaaacacg cgcagaatcc atacggaaag tggcgaactt cacgctgcgt tacaaaaagc
     9661 aaccgaaggc gcagccgaac agcgcgagcg cagctgcgcg aatatcgccg agcttgccga
     9721 cttggcaaag gaactcgcgg atttgatcag ttccacacac actgaattgc ttgaaaactc
     9781 gcgcaaaatc agtgaagaga gcggtggaat tcaaaccgcg atacggcaag cggctgagga
     9841 ggccactgat caacgggaac acgacttggc aaaactcgct gagttgtccg gcttggtgaa
     9901 agatctcgcc gaatcggcag gcgtagcgca gctcgaactg ttggaaaaca cgcgtaaaat
     9961 ctatgaagag agcggtggaa ttcaaaccgc gatacggcag ttagccgaag gcgccgctta
    10021 tcaacgtgaa cacgatcgcg cggaaatcgc cgggctgtcc ggcttggtga agggcttcgc
    10081 ggaatcattt ggcgccacgc aagcggaaat gctcgagaat gcccgaaggg gcaatacgga
    10141 aagcgagaag cttcataccg ctattcgcga agcaaccgag gaagccgcga aacagcgaga
    10201 acacagctgc gcgaatctcg ccgagctatc cgacttggcg aaggaactcg cggatttgat
    10261 cagttccaca cacactgaat tgcttgaaaa ctcaagaaga atcaatgaag aaagcggtgt
    10321 gatacgaacc gcgatacgga aagtatccga agaggtcgcg agtcaatggg aacagaatcg
    10381 aacagaagta gccgagttag ccggattggt taaggaactt gccgcatcga ccagctcaag
    10441 acaaaccgag tcgctcgaaa aaacaagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnngg aacttgccgc atcgaccagc tcaagacaaa ccgagtcgct cgaaaaaaca
    10621 agcaaaatca gcgaagagag cggtgaaatt caactcgcga tacggcaagc agctgaggag
    10681 accgcgagtc aacgggagca cgacttggca aaactcactg agttgtccgg cctggtgaaa
    10741 gatcttgccg aatcggcagg cgtagcgcag ctcgaactgt tggaaaacac gcgtaaaatc
    10801 tatgaagaga gcggtggaat tcaaaccgcg atacggcagt tagccgaaga tgccacaaat
    10861 caacgggagc gcggccgcgc ggaaatcgcc gggctgtcca gcttaacgaa agaactcgcc
    10921 gatttgatca gctcgacata caccgaactg ctcgaaaaca caagcaaaat caatactgag
    10981 agcggtggaa ttcaaaccgc gatacggcaa ttagccgaag aggccgcaaa tcaacgggag
    11041 cacgaccgcg cggagcacgc cgagttttct gacttggtga agcgcctcgc cgattcgacc
    11101 ggcgacttgg gcgcacgcct gcgctcgctc gttgatgaga aaaccggttt atcacggctg
    11161 gatcccgcat tcgtgtcaac ggttgaccgc aagcttggca acctcattgc gtcgtttggc
    11221 gaaggaaatc gggagacttt ggatgccctg agagagatgg atgaaaatgt ccacgcctct
    11281 ctccaggatt cccgaaacct cgcggtttta acgcagaaaa ccgttgaagc cctacctaag
    11341 ctgctgaaag gccattacag cgagtcgatc gcaaccctga ccgaaaaaac cgccgcgttc
    11401 gagagcgctc tgaccgcgct cgatgaactg gaaaagaaaa cggccgagaa acaacaggcc
    11461 ttcgatgacc gcctaactgc ccttgagaag cgcgtcaaag aaaagatgga tcgcacctac
    11521 ttctttcaat tgatggtatt cgcgagccaa tgggtggcgt tgatcgtgtt catgctcttc
    11581 tctttctgga aggtatagcc ggttccacgc tcgataggtc catccaataa aaccaaacgg
    11641 ggggcacaaa atgacacaga tatacgcgcc cggtatgcgg atacaggtac gcggtgagga
    11701 gtgggtagtt cggcgggccg attacaacgc ggataggaaa tacattttaa gcgtgacggg
    11761 cgtttccgat tttgtgcgtg ggcaggaggc cgcgttcctg tccgatctcg aggagatacg
    11821 cgtgatcgat ccggctgata cccagttcgt tgtcgaccgg tcgccgatgt ttaggaagtc
    11881 gcggctctac atcgagagcc tcctgcgtcg acgaaccccg ccgggaaacg tcttgcgcat
    11941 cgggcacaaa gcggcgatga atgtgatcga tttccaactg gagcccgccc agcgcgcctt
    12001 agcccggccc cgtcaacgca ttctgctggc agacggtgtc ggcctcggga aaacgctcga
    12061 agccggcgtg ttgatgagcg agctgatcgc gcgcggtaaa gggaaacgta tcctcgtcgt
    12121 cgcgctgaaa agtatgatgc tccagctcca aaaagaactc tggaccggtt caccattccg
    12181 ctcaaacggc tggactcccg agggatccaa cgtattcgcg ccaacattcc gacgaaccat
    12241 aacccatttt actatttcga taaggtgatc atttcgatcg acaccctgaa gatggaccgc
    12301 gagtaccgaa cgctgctcga aaaaacccgg tgggacatca tcgtgatcga cgaagcgcac
    12361 aacgtcgcgc gacgtggcaa aacgcaagcg caacggtccc gcctcgccga attgctctcc
    12421 aaacgctcgg acacgctcat cctcctgtcg gcgactcctc acgatggccg cccgcagagt
    12481 ttcgcctcct tgatgaatat gttggagccg accgccatcg ccaacgagga ggactaccgg
    12541 cgcgaggaca tccaagggat ggtcttccgc agattcaaaa aggatgtgca gaaccaggtt
    12601 tccgcccatt tcaaggaacg tgtgatcagt cgggaaacgt gcgaggcgtc cccgttagaa
    12661 gaagcgttct acgacttttt cgagactctc aatctcacct ggatcgaccc cgctcgcggg
    12721 gagaccatgc tgtttaaaac gctgctggaa aagtccctct tctcctcacc ggtcgcctgc
    12781 gaaaaaacgg cgcaggcgcg catcaataga ctgaaaaaag aggggcggtc cgagagcgcc
    12841 tccgatatcc gcgctctaga gaccctcatc gaccatttga aaaaaatcac acccgagcat
    12901 ttttcacgat accgccttct ggtccgcctg ctccaaagcc ccgaatacgg ttggaatcgc
    12961 gagcgcccgg acgaccgcct cgtcatcttc acggaacgaa tcgagacgat gaggtttttg
    13021 gccaaacgcc tgaaagagga cctccggctg agcgatggcc aactctcctg tctttacggc
    13081 ggtatgagcg atgtggacca acagcaggcg gtcgatgagt tcggcgcgac cgactccccc
    13141 gttcgtctcc tcatcgcctc cgacgtggcg tccgaaggca tcaacctcca ttttctatgc
    13201 catcgcctga tccatttcga catcccgtgg tcactgatgg tgttccaaca gcggaacggc
    13261 cggatcgacc ggtacggcca agaaaaaacg ccggacatcc gctatctggt gaccaaagcg
    13321 aaaaacgagc gcattcgcgg ggatatgcgc atcctggaaa tactcatcga taaggaagag
    13381 caggcggaaa agaatatcgg cgaccccgcc tccctcttgg ggcggtacga tgtggaagag
    13441 gaagaacgga cggtagcccg cgttttcgaa aagagcctca gcgtgaatgc tttcgagcgc
    13501 ctgctatcgg ctcccgaaaa cccgtttttg gcgatgatga acgcgggttg gaaccttccc
    13561 gacgaagccg cgaaggcgac gaacgtcgaa aaggccgatc ccgtgaccct gtttgacgaa
    13621 acgagttatc tgaaaaccct cttggaaacg atctctaaca gggaaaccat acctctggaa
    13681 gccccacgag tcgacgtgat ggaaatccgg atgtccgaat cgatgaagcg gtcgctggag
    13741 ccgcagatac ccgaggaggc gtttccgagc gacgactggt tgcgcctgac gacggacaag
    13801 aaagcgctga tgcgtgagat caaagaaagc cgacagaagg cgcaagacac gtggccgcgc
    13861 tcgcactacc tctggccgct tcaccccatc atcgattgga tgaacgataa agcggggctg
    13921 tttttcggaa ggcaggaggc gccggtactt ttcgtcccct ttgcggagaa gcggaccgcc
    13981 gtcttcatca tctccgggtt gattccgaat cgacagtccg agccggttat cgaccgatgg
    14041 ttcgtcatcc gcttcgttga agataacccc gccgagtttt tgtccccgga ggattttctc
    14101 cgttcagacg tattcaaagg cgcgcttccg aaccaggcct cgaggaacgc agaagtttcg
    14161 gattgggccg ctaagttact gccgacggcg attggcctgg caaaggaaaa aatgaaagcg
    14221 gagcaagagg agttcgaaga gacgatgacc ccgaaactcg atgaagaaat cgcgaagctt
    14281 aaagagctgc gaaagaaaca cgacgacctg cttcagagtt acgacagatc tgacggaagg
    14341 aacgcccagc gctacgagat agaaaagcgt cggattgatg agatattcaa tgaatttcaa
    14401 cattggctcg taaacacgat gaagctcgag aaagacaacc cctatatccg cgtggttgcc
    14461 cttctcctga gcgaatgagg ggagcttatg gcgatcgacc tgtccggtat ccacaacgtg
    14521 aacgaatact acaccgcgca ctacctgagc acgattttcg aggaaaacat ccgagaagcg
    14581 ctgtcgatgt ggaaggcgca ggcccaggag tcggaagaga aggagccttg gcgcaagatc
    14641 aagtccctgg gtagggcgta cttcaaccga ctcgacgagt acacgcgcga gcgcgacgaa
    14701 caccgacggc gtgaaatacg gcgctcgttt ctgaaagagc tgttcagcgc cctgggtttt
    14761 ctattctcgg aggcggatga agagttcatc ggaaacgacg ggatcacggt acctgtatgg
    14821 caaatcctgc gaaaacccga cggcgcgccc tacctcttgc tgatagaaac agatgaaacc
    14881 gataacggca gcgcgctcga aagcgttgcg gaggccttcc ccgattgcga ctacgaagag
    14941 gcgttgaacc ggtacatctt caacgcgccg gagccgcccc gttgggtcat cctctgctcg
    15001 atgcgcgaga tcgcgctgct cgaccggtac aagtggaacg aaaagcggtt cttccagttc
    15061 attttggatg aaatctacgc gcggcgcgag gacacgacct tccaagcgat ggccgttttg
    15121 ctggacaagt caagcctcgc cccattcgat ggggaacccc tgctggaccg tttcgacgac
    15181 acctcccaca aacacgcgat atccgtgtcc gaagacttga aatacgcgct gcgccgatgc
    15241 atcgaatggt tgggcaacga cgcggtgcgg gatatgaaag agcggcagaa ggtgaaggtg
    15301 tacgataccg acctcgcgga gcagctgacc ctcgagtgcc tgcgctttat gtaccggatc
    15361 cttttcatcc ttttcatcga atcacggaaa gagctggaat acgcgccggt ccaatcggac
    15421 gtttatatga agggatacag cttcgagagc ctccgcgacc tcgtcgacaa ggttcggttg
    15481 gagggcgagg aaagtcgaaa cggctatttc ctcgacacgt cgttgaaaaa actcttttcg
    15541 ttgatctacg aagggtatcc gaaacgcaac ggaacggtaa aaaccgctcg cgccgaggcg
    15601 ctcggcttgg aaggcgtcgg cgcggacgcc ttcgagattc cgccgctgca gtcccacctc
    15661 ttcgatccgg aaagaacgcc cctcttaaac cgcgcgcgtt ttaggaacga aacgctgcgc
    15721 gacgtcatcc gcgaaatgtc gatcacgagg ccgcgaggaa gggagcgtgc cggacgcgtt
    15781 tcctactccc acctggggat caaccagctt ggcgccgtct acgaggggct gctgagctac
    15841 cgcggcttct tcgccgagga agacctctac gaagtacagc cgaaaggcga atcgtgggat
    15901 gagctgcaag tcggctattt cgtccccgcc tcgcggctcg aagagtacga agaaacggag
    15961 cgggtacgca accccgacgg cagcctgaaa ttgtacccga aaggcacgtt catctaccgg
    16021 ttggcgggca gggaacgaga aaagtccgct tcgtactaca cgccggaaac gctcacccaa
    16081 tgcctcgtca aatacgcgtt gaaagagctg ttgaaagaca aaaccgccga cgagatcctc
    16141 gaactcaaaa tctgcgagcc cgcgatgggc agcgccgcct tcatcaacga agcggtcacg
    16201 caactcgcag aagcctattt acagctgaaa cagcgggaga cggggcaaac gatcgcagtg
    16261 gaccggtacg cggaagaaaa gcagaaggtc aagatgcgcc tgtcagaccg gaacgtctac
    16321 ggaatcgacc tgaacccgat cgccgtggag ttggcggagg tctccgtctg gctgaacacg
    16381 atctacaagg gcggctacgt tccgtggttc agaacgcagc tgatgaacgg caattccctg
    16441 atcggcgcgc gccgacaggt ctaccgtccc gatcagattc gggagaagaa gaaggagaaa
    16501 cagtggcaca ccgtcgcccc cgaacgcgtg gaaccgaaaa cgacgagacc ggcgggcagc
    16561 gtctatcact tcctgctcgc ggatcctgcg atggcgacgt acgaggacaa ggtcgtcaaa
    16621 aaactgaaag ccgaagaact caagcggttc aaagagtgga acaaagactt ctctcgaccc
    16681 tacgacgcgg aagagatcaa aacgttgcag cggctgagcg accaggtcga cctcctctgg
    16741 gaaaaacacc tgaaggcgcg aaaaaaggtc gaagagctca ccgagagcgc gatatccatc
    16801 tttggacacg aggagaaggc gcgccgccgt ccgtacacga cgaaagagaa ggacgcgatc
    16861 tacgagagcc tctttcatac cgaaaagatg gagaacgccg gcccatacgc ccggttgaag
    16921 ttagcgatgg actattggtg cgcgctatgg ttctggccga tcgataaggc cgatctgttg
    16981 ccgtcgcgtt cggagttcct gatgcacctc tcgctcatcc tcgaagtgaa tctgataccg
    17041 accgcgcccg cgcagcgacc cctgttcgtc gagaacgacg cggaaaaaca gttgcagacg
    17101 ctcttttccg acctggacct cgtggatttg gacgcgctct gcgaaaagct cgagccattg
    17161 cgcgtggtac gcgaggtcgc gcgacggatc gcctttttcc attgggagct ggagttcgcc
    17221 gacgttttcg acgagcgcgg aggcttcgac ctcatccttg ggaacccccc ttggatcaaa
    17281 ctgggatggg aggagggcgg gatcctctcg gacgcggaac cgctcttcgc gatccggaaa
    17341 tactccgccg atcaggtcag taaactacgg aacgacagtt tcgagcgcca gccggagctg
    17401 ctcgaggact acgtgcggga gtatgaaacg atggaggcga cgaagggctt cctcaacgct
    17461 gtccagaact acccgctcct tcaaggatca cagtcgaacc tgttcaagtg ctttctaccg
    17521 cagagctggc tcttcgcgaa ccgaaacggc gtccagggct tcctccatcc ggaaggggtg
    17581 tatgacgacg cgaaaggcgg cgcgctgcgc cgcgagctct atccgcggtt gcggtatcat
    17641 tttcaatttc aaaaccaata tatgcttttc ccaattgggc accgagtcaa atatgggatt
    17701 aacatctaca aatccaattt tcacgagata tcttttgaca gtctttccaa cctattttta
    17761 ccaaagacga tcgatgattc cttcgaccat caaggcgatg aaccgcttga gggaatcaag
    17821 aacgaacagg atgaatggaa cttgaaaggt catcatctaa gggtcgtgca cgtggatgaa
    17881 ggggcgctaa cggtcttcag tcaacttttc gacgaggagg ggacaccccc cttggagtcg
    17941 aaactcccct ccgtacacgc ccaacccatc gtcggcgtgt tgaagaagtt cgccgcctat
    18001 ccgcatcgcc tgagggatct caaaggcgaa taccactcta ctgtaatgtg ggatgaaacc
    18061 aacgcccaaa aggacggcac gatcaaaaga gagaccatct ttcctaagac cgcggaagaa
    18121 ctgatccttt ccggcccaca ttttcacgtg ggaacgcctt tttacaaaac gccgagggct
    18181 atatgcacag agaagggtca ttacgatcct ctcgatctcg ccgaactccc cgacgattat
    18241 ctgccgagaa cgaattatgt tccggcgcca gacagaatgc tctaccagaa ccgcattccg
    18301 accgcgaaat ggagtcttaa ccaggggaac cgttccgatc ttgtgaccga ttatttccga
    18361 atgatccatc ggaggcggtt gagccaattt ggagaaagaa ccttcatctc cgcgttgata
    18421 cccaaaaata tagcccatgt gcataccgtg atatctacct ttttcttgga accgcggcgc
    18481 ctgcttcaag ctttcgttcc ggcttgttcc cttctcagtg actttttcgt caaaaccagc
    18541 ggaaaagaag attttactac cgggaatgtc gatgacctgt tctatttgga agacgactct
    18601 cgttttctat acgcgcttcc acgcgccctc accctcaact gtctcacgac cccttatgcc
    18661 gagttgtggg aggaactgtt cgatccccgg tttcagaaag atacctggac gagcgaggac
    18721 ccgcggctcg accgggattt tttccggaac ctcaccccca cctggcagcg ccacaacgcg
    18781 ttgcgctacg actacgcgcg tcgccaggcg ctcgtggaaa tcgacgtcct caccgcgatg
    18841 gcgctgggat tgaccttgga ggagctgatc tccatctacc ggatccagtt tcctgtgatg
    18901 cggcagtatg agaaggacac ctactatgat cggaagggtc ggattgtgtg gacgaacagc
    18961 aaagggctaa ccggcgtcgg gcttcgggaa cgctccgagt gggaggccct gcaggaactg
    19021 aaggacggtg agagctgttc gaggatggtg aaggacgata cccggcccgg cgggcccttc
    19081 gaacgcgagg tgcgctattt cgcgcctttc accctcgccg accgcgagac cgattacgcc
    19141 gtcgcctggc gtgagttcga gaggcgcggg ttgtaaaatg cccgaatacc ggatcgcgct
    19201 gccgccgaca gtttggcgtc tctatcgcga cgtgaaaccc cgggccgcgc aatatggcgt
    19261 acagaggagg gacgtatgat tccatcgatc gtgtgcgaac aactgcaacg cggtttgacc
    19321 gagtacctca agaccacatt cccgatctcg agcgaggtct tcgccggatg catcgaccgg
    19381 ttcctcgatg cccgctcgat gttcaagggg ccgtatgtca ccctacggat gcctttccga
    19441 gaggctgcgc gagagaatcc ctggtttgag gcgctcgaac tgcccttcgc cccgtacctc
    19501 caccagaagc gcgccttcga ccggttggcg ggggaggccc cgcgttccac cctcgtcgca
    19561 acggggacgg gatccgggaa gaccgaatgt ttcctctacc cgctgctgga gcattgctgg
    19621 cgccatcggg gcgaaaaagg gatcaaagcg ctcctgatct acccgatgaa cgcgctcgcg
    19681 acagaccaag cgcgccgcat cgcggggctg atctatagga acgcgaagct gaaagggaac
    19741 gtcgtcgcgg gcaagtacac cggggatagc gggccgggcg cttcccgcgg aatgggggag
    19801 caagagctga tcaccgatcg cgagatgctg cgccagaatc cgccggatat cctgctcacc
    19861 aactacaaga tgctcgacta cctgctgatc cgttccgacg acttcccgct gtggtcaaaa
    19921 aacgggccgg aaacgttgaa atacatcgtc gtcgacgaat ttcacacctt cgacggcgcg
    19981 cagggcacgg acctcgcctg cctccttcgg cggttgaaga agcggctgga gatccccgag
    20041 gggttcctgt gctgcgtggg cacctccgcc acgctgggcg acgcccaaag ccaaaccgaa
    20101 atggtcgcct tcgcctccga agtattcggc gaacccttcc ccgaggatgc cgtcatcgga
    20161 gaacagcgtc agacggcgga cgaattcctc cggggaacgg agattcaata cacggcccta
    20221 ccggataaga aggcgctgtc ggagatcgca aacgcgatga cctcgggaag cgaacgagat
    20281 ttcatacgcc tggcgagcct ccgctggttc ggagacgcaa cgctcggcga cgatgataac
    20341 gacgcgcgag gtcgggagct gatgggcgaa cgcttacgcg gacactattt cttccacgag
    20401 ctcctgcgcg cgttggaagg cggggcgtcg agttatgaag agctcctcgc cgagctatct
    20461 cgccgcaatc ggatattgga cttttattcc aaacccgatc gtcacctgtt gcttctctcc
    20521 ctgatcggat tggtttcata cgcccggatc ctacaggtac acgttcagat atggttccgc
    20581 gaactccggc gcctattggg gaaagtggac cccgagaagc cggacttggc gctctccgac
    20641 gacttgaccg aaggagagcg tggccaatat ctacccgtcg tgaactgtcg cgattgcggc
    20701 gtgaccggtt gggtttcgat gatgagcgat tccttcaaca ccaaaatcat cgatcttcgc
    20761 ggcttctac
//
LOCUS       DAOPXN010000016        20696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2584_length_20696_cov_5.567027, whole genome shotgun sequence.
ACCESSION   DAOPXN010000016 DAOPXN010000000
VERSION     DAOPXN010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2584_length_20696_cov_5.567027"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    527..626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1500..2078)
                     /locus_tag="PKM49_01735"
     CDS             complement(1500..2078)
                     /locus_tag="PKM49_01735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNY81568.1"
                     /translation="MKRTTIFLTRHASTLWNEEARFQGSLNSPLSLKGRDQARQLGLV
                     IRELSPEVLCTSTQLRSQETGAIALEAAGLSLSIDRYPELNEISLGYWEGKTAAWVKE
                     RDAEVFKEYVQNPQTFKHPNGEGFQDVLSRALAGMEKILEIHSGKRVWVITHGFVLIA
                     LLIYLRNIPAEHYALKMRLPGNAEVLHTTWLR"
     gene            complement(2075..2668)
                     /gene="rdgB"
                     /locus_tag="PKM49_01740"
     CDS             complement(2075..2668)
                     /gene="rdgB"
                     /locus_tag="PKM49_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNY81569.1"
                     /translation="MKILLATTNRFKVREITEIIPDVEFLAISDFTDHWEVEEVGATF
                     EENSLLKAKTAAHYFGIISIADDSGLEIPILSYFPGVQSSRFLEDRPFEEKMKAVLER
                     MSSFDKPEERVARFRTVATYYDPLTGQSLSAEGVVEGSIAERISGDNGFGYDPIFIPK
                     GFDSTFGELTDVIKNNFSHRSRAFHKLFERIKSEALP"
     gene            complement(2665..3339)
                     /locus_tag="PKM49_01745"
     CDS             complement(2665..3339)
                     /locus_tag="PKM49_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81570.1"
                     /translation="MEQTPQEVVDRLVNEKGLDALDGLMDLIKSSEDAEVLSIVQEAL
                     VRLGSAAKPRILEFLEKETLESASLPGLLILVETLGDIGARGDIPVLYSYLKLFEQEI
                     DQLYVYEAIAKLGGGKELLSLLELLLFEDEEKDLLRDHIILILSYIRDRQALSFLVRL
                     HALNDSNGEQEELILLSVMSLLTQNPAWVGELNASSEGRKIMEKLSAKIKGQIDESVT
                     QRENPL"
     gene            complement(3471..4028)
                     /locus_tag="PKM49_01750"
     CDS             complement(3471..4028)
                     /locus_tag="PKM49_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNY81571.1"
                     /translation="MAKKTIVYLALLVLATALFAGWTIEFGFGPIVVTPQPQPQPQPQ
                     PVYPYPYPYPYPYPTPPPAGGIRLGISTVDNNGYGVLVTEVLPGYPAYGQLFPGDIIR
                     SVQILQGQVMVYNNYVQAYGFYGYPQTPSGVWVYSSTQLQSMILSAPWNSSIILWIQR
                     YGSPMMLVIQLVNANSPPIFFSINP"
     gene            complement(4188..4910)
                     /locus_tag="PKM49_01755"
     CDS             complement(4188..4910)
                     /locus_tag="PKM49_01755"
                     /inference="COORDINATES: protein motif:HMM:NF015426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81572.1"
                     /translation="MGRSDRRAYAYFLYPKLGRLLPESRSRLDAIQTFSGAGSGLQLA
                     LRDMEIRGIGNLLGFEQHGNINTVGLHLYREMLDAALVKRGLKEEKKTKEEPEETIRV
                     EIKGFPLNCVLTDLYIASQIERMKIYRKIALSRTSADIEAVELELVDRFGPLEPNARE
                     LLLYSRIRILADALGIKSIESLKRSRNVRLAFIDRLALERFEWGSLRGFSNVEGLEVW
                     LFEIDPRHIAQLFEDNVSRRGL"
     gene            complement(4944..7382)
                     /locus_tag="PKM49_01760"
     CDS             complement(4944..7382)
                     /locus_tag="PKM49_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNY81573.1"
                     /translation="MDCAIEKLPLTGKHTAYLKTDLHDDFLKRLISRLHSLSKPWVIA
                     FEDMKSLQDFGSLDSPQDLCLFPPFDLLPFEQVEGSIPTLKNRMKTLYRLGSFERFNG
                     GILTTIPTLLQKIVDPRLLVTSSLTFKKGGYITGDGRPRYDRESVIQHLTESGYRRVY
                     TVENLGEFSARGFILDFFSLLDDFPTRIEFFDDEVSDVRFFDPQTQLSLPRGVDSTEG
                     CQRQEGERLIPPFRETFFKEENLECFRNRIADLKKRYPNSLFLGTLEQKPDRFPTLGG
                     MLLPTQRALVNLLPKDTVFFYVRLSQALERFRAYEKELFDIYPQAEYKEIYLKYLRVS
                     ESALLSRENVTVLTESRGPSVSLGDQAALFADYTVSSDGLAPVKRKSQTSAFLEYPSQ
                     IIASSEDLEKGDLIVHEDFGIGRYQGNEVISNYAGTREYLKIEYADQVNIFVPVENLD
                     RVHQYIGDETLVGLTRLKGNGWIRTRNKVREDIEEKVEELIQLYAIRKATTKPPVFGD
                     FELESKFAASFPYVETKPQARSIEDVYHDLAREYPMDRLIFGDAGSGKTEIAMRAAFR
                     MALNGKQTAMLVPTTILSKQHYETFSERFSAYGAKVILINRFVTEKERKLALSMIADG
                     SADVIIGTHSLLSSRIKYKDLGLLIIDEEQKFGVAQKEKIKKKKTEVDVLTLSATPIP
                     RTLYMGFSGVKEMSVVDTLPPGRLPVEVLVAPWEERIVKTAILRELSRGGQVIYVHNR
                     VEDISESLDQVRALVPEANVGLAHGQMKKSIFEQTIREFYEGQIDILVCTTIIESGVD
                     IPNANTLVIDDAQR"
     gene            complement(7397..8233)
                     /locus_tag="PKM49_01765"
     CDS             complement(7397..8233)
                     /locus_tag="PKM49_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81574.1"
                     /translation="MFDKIKDFFKDLADSVKKETGAISVLGTDFEFDTGIGDIYAPLT
                     DAIALEGFHAYGTELKITDLEEYSDALCVVKGFNEETVGRFDRIVKTAVFTEEASVSS
                     IEPPGKEVALRDFTPAGTDTRVYDESPEDTSLSMEQILSFPLYVVRGISVYSEEPLEA
                     PGLRSEGLFASIKTNASDRIIARFPMKRSSVSRENYSTSNIKNALSFILDRIDHKPRL
                     KWVGIFRDVPINRIKQLTFHESTLSFTLYSFDRRSIYILDVLAVFDEVTEKYHIAPIV
                     RG"
     gene            complement(8218..8727)
                     /locus_tag="PKM49_01770"
     CDS             complement(8218..8727)
                     /locus_tag="PKM49_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81575.1"
                     /translation="MADVASVFGYLGIEKKSDETYVGGILISDYYGIPVEFKYTEPIK
                     PTNLQKILYGKSIEKYLTVDVLSKKLIISIQEKPRFILVRDMTLLQTQSKYPLIFLES
                     IKKETTDADATDDRYRSDMIGKEYKIVYVNNLTSDEHQWLTTISKEIDILEPFSRLKE
                     ALDYVCSIK"
     gene            complement(8766..9083)
                     /locus_tag="PKM49_01775"
     CDS             complement(8766..9083)
                     /locus_tag="PKM49_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81576.1"
                     /translation="MMTGPLEMQTSFVKPAEAAQSVSRQLITQEAAQQALASEMSKKT
                     KESTHTTPAAQKAEQEGVKHSTDQEQGAAQGELSRKKKEEEKQPPKKPEDDYRGHFLD
                     VRG"
     gene            complement(9105..9863)
                     /locus_tag="PKM49_01780"
     CDS             complement(9105..9863)
                     /locus_tag="PKM49_01780"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HNY81577.1"
                     /translation="MIKNKRLDSSYSQNRLTKDQLIQMILPKIKALASNVKSTLPKSV
                     EVSDLVQEGVVALINAFDRYDETKGASFTTFALTRVKGAMYDYLRSIDWLPRKTRRQI
                     KEIEKAYFELEAKNGATPTEEEIAEYLKLPLEEVQTTRMDMNCKQILDLDNYLSPDET
                     DFTETIVSEELNPEQQFEKDERRDRLKSFISSLTEREQLAISLYYIEELNLKEIALVF
                     GVTESRVSQIMSKALRTLKHRIEEAEAEERRQFF"
     gene            complement(9913..10401)
                     /locus_tag="PKM49_01785"
     CDS             complement(9913..10401)
                     /locus_tag="PKM49_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932012.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNY81578.1"
                     /translation="MEEKAIIGIGEYKSGKNPLVLVTLGLGSCVGVCIRDPAKGVGGL
                     IHVMLPESGGKEVSKPGKYADSGIEVLLKEMGALGANLSKLEAKIAGGAAMFKSTGSS
                     MEVGKRNTEAVKNVLAQKKIRLLSEDTGGTRARSIEYNIQTGILQIRKVGGGEKTEIL
                     EL"
     gene            complement(10407..11021)
                     /gene="cheC"
                     /locus_tag="PKM49_01790"
     CDS             complement(10407..11021)
                     /gene="cheC"
                     /locus_tag="PKM49_01790"
                     /inference="COORDINATES: protein motif:HMM:NF041093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheY-P phosphatase CheC"
                     /protein_id="HNY81579.1"
                     /translation="MSYYAQLSPQHLDVLKEIGNIGTGNGATALSMMTGKKVEIKVPS
                     VSVIEFSRIPLFFKDPEKVTVGIQLGFHEELNGKLLLLFDSEPGRRLLELLIGFVNED
                     LTTMDEMQRSVFSEIGNIVCGSYLVSLTQFTNLFVNSEVPQITVDMVSGIVAESCLEY
                     AEEEDHTILIETNFQIEGQQNVEAFLFLILKPESVKKLLESLGM"
     gene            complement(11021..11710)
                     /locus_tag="PKM49_01795"
     CDS             complement(11021..11710)
                     /locus_tag="PKM49_01795"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake domain-containing protein"
                     /protein_id="HNY81580.1"
                     /translation="MAYFNTQIKASEGLRIGKPLIIDVSDPLLEGVYKSSIYDIDFNK
                     KVMKIGMPSSQGQFVPLPAGTRLYVKMVDKSSMYVFQSMVISYEKDQEGFLVTYILFP
                     DNLRKIQRRQFVRVPFFVEGDFIRLKTNETHKFITKDLSAGGLLMVSPCEMTVSEKVV
                     VNMVVCESITLDGVTSQIVRSDRNVQTKHWIYGVEFKDLPRSIEDRIVRYVFVLEQEY
                     RKKIKEEGANP"
     gene            complement(11722..12354)
                     /locus_tag="PKM49_01800"
     CDS             complement(11722..12354)
                     /locus_tag="PKM49_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81581.1"
                     /translation="MILIQNLSKLLKLSCWKSMLELPGRSLFCVFLGTYKDLLLFESR
                     VFQISADLRNDSEIVYQIFLPLGIIKGEGRLKEQMVKDDPVYGKVKRLLVSPGQTMVS
                     QKRNYKRYFLIEKGTFESRGESRQVIVKDVSMAGIGVSCQNRLLAEEGTIRIFGIQRP
                     FHVKKAYEFTNYNFFEYGFLLRLADEEQKNELKKKLLVFHQKLAKLGVSH"
     gene            complement(12362..13210)
                     /locus_tag="PKM49_01805"
     CDS             complement(12362..13210)
                     /locus_tag="PKM49_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HNY81582.1"
                     /translation="MPDQLETLKKVVAAKNFEIIVVASGKGGVGKSVIAANFAVALAK
                     NQKSVLLFDADVGFANVDILLGLTTKNTLRDYLVGKMELKDVLYPTSYGIWLLSSGTD
                     VGDLIAFRNESKLRLMDDFLKLVVDMDYAVVDTGAGFNEELVAFYQAADKLFLVTTPE
                     PTALINVYTLAKVLATKGIFPELYLIMNQSRNTSKDTQVLERFSYVLEKFAGWTIAEK
                     LIVREDSGVSASVLKQAPFIETQRHSQPAQAILRLSKMILKSQYKEDEEPNGFFVKFK
                     KLFGIK"
     gene            complement(13185..14537)
                     /gene="flhF"
                     /locus_tag="PKM49_01810"
     CDS             complement(13185..14537)
                     /gene="flhF"
                     /locus_tag="PKM49_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNY81583.1"
                     /translation="MKIKKYVVEDVKDALKQIKRDLGEDAVILGTRKFKSGGFLGFGS
                     RTKYEVTAVAEEKKEEIKSKTPPKDEEWIDSKKLYELKRILSKNVQAQAETSPSPKER
                     PKTPSVPSTGYSVGLPKIEKVDEDSSFTRLSGVPATEWGGGDDAGARDVSGLRKDMLA
                     MKRMIQDLSVKIGKEHYLPGLPERFRRIYEALIDQEVNQDLSRRMVESLRIVTNDGMD
                     NTMEVERKFSEVLSPAIRTDNFIKNHEKGDILFFIGPTGVGKTTTLTKIAAMLSLEMR
                     KRIGILTIDTYRIAAVDQLKTSADIMGIGVGVAYNPKELSMLLERFKDFDLVLVDTAG
                     RNQKNELQMNELSRYLQIVNPKYKLLTMSMNTRSKDMYSIIEHFSVIAPTHLILTKMD
                     ETSVYGSFLELSSRYDIPVCFITNGQRIPEDIMIADSKRLASFIVKEVLTNARPVGNA
                     "
     gene            complement(14534..16636)
                     /gene="flhA"
                     /locus_tag="PKM49_01815"
     CDS             complement(14534..16636)
                     /gene="flhA"
                     /locus_tag="PKM49_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNY81584.1"
                     /translation="MKYFDIIITLLIVGIVLLMILPIPTFLLDIFQIINITISLVILL
                     STMYVGNALELSSFPSILLVITLFRLALNVNSTRLILLQGKNFDGRVVKAFGNFVVQG
                     NYIVGIIIFLILVIIQFIVITKGSERISEVAARFTLDAMPGKQMSVDADYNAGLITEE
                     EAKKRRNDVRREADFYGAMDGASKFVRGDSIAGIIIVFVNIIGGLIIGAVQQGLSIGE
                     AAEVFTLLTVGDGLAAQIPSLLVSTSSGILVSRAASEDNLSRDMMKQLTSDNRVMFLT
                     GGVLVFLSLFTPLPTFSTLLIGVLLIAMGFMASRETKKLAPETGGPGGPEPEGFAGLK
                     GPPPGEPRPKGEQPAPFMTTSAEVSEIIQTDTIDVELGYGLLPLADPTQGGDLLDRIT
                     LVRKQLAYEMGLILTPIRVRDSVLLKANEYVIKLKGTEIARYELWPNRLLAINPGTAK
                     GILPGISSREPAFSLEAYWIDESQREFAINSGYTVVDPPSVFATHLSEIIKKYAHEIL
                     GRREMSLLIDGLKEKFSSLVNEVIPDGLKMFEVQRILQNLLKERVSIRNMPLIFETLT
                     EHIDKTKDIDYLTEYVRRGLKRQIVLDARSDDGALHAVGLDPSLERQLVNSIRETPEE
                     RYLAIDPEMLKKLEGTLSNALEKAMKKGFMPIVVCSGSIRYYLKRSIERALPGVIVLA
                     YEELVEDIPFSVEAVINL"
     gene            complement(16639..17760)
                     /gene="flhB"
                     /locus_tag="PKM49_01820"
     CDS             complement(16639..17760)
                     /gene="flhB"
                     /locus_tag="PKM49_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNY81585.1"
                     /translation="MDESNLQMVLEEIRLYIDLQLFADPDKTEKATPRRRQKAREEGQ
                     ITYSKDLATAAVFIGIIVFLRFIVPKLYENLTRLVVFYLSLPNLGDIADFKDPFLNLF
                     IEGKDFFLFLAFLLLAAFAIALMTGMLQTRLLFTLKGIKFDLNKLNPINGFKRMFSLR
                     SVVELIKNIGKLAILGIIGWSFITGLWNTLFLLPDYSTMDSMVLIGDSLYQLAIRIGF
                     ALVILGFVDYFYQRWEFERSIRMSKQEIKDEYKNIEGNPQIKAKQRQMMAELAKRRMM
                     EEVPSATVVVTNPTHYAVAIQFDLDTMSAPVVVAKGIDFIALRIIEIAKEHDVPIHEN
                     PPLARRLYETTEIGEEIPAELYQAVAQVLAYVLKTVGKV"
     gene            complement(17730..18509)
                     /gene="fliR"
                     /locus_tag="PKM49_01825"
     CDS             complement(17730..18509)
                     /gene="fliR"
                     /locus_tag="PKM49_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HNY81586.1"
                     /translation="MDFDVQQFVFRYFLSWSAIISRLTGLILYSPFFGGHYFPPLIKV
                     LFVVFTSYILIPAVGTPIPLTTPVGDIVLIEMLNFTFGFAAGLVANILFWAVDFAGDI
                     YGYQLGFSAATIYDPQTETESVILSQMTSIGFLFMFVLMKGPLILFQVLVESFRLVPL
                     TIMRMDANFPLEFSTLMGNVLIFGLQIGIPMIFFMILVTFVLGIMSKLLPQLNVFIVG
                     LPLKVLVGMIILVGLLPIWADVVVRYTRELTVWMNQIFKWF"
     gene            complement(18521..18787)
                     /gene="fliQ"
                     /locus_tag="PKM49_01830"
     CDS             complement(18521..18787)
                     /gene="fliQ"
                     /locus_tag="PKM49_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HNY81587.1"
                     /translation="MTIETFLDVFMEGIYVILYTIAPTMLLSLAIGLLIGIFQAVTQI
                     NDQTISFVPKVLVVFLSLVIFGGWIFQKLMDYIADVMTYYMNLI"
     gene            19370..20605
                     /locus_tag="PKM49_01835"
     CDS             19370..20605
                     /locus_tag="PKM49_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY81588.1"
                     /translation="MRPITAVLVGAGNRGTHAYGDYALLKPESLRIVAVADPDGTRRK
                     RFQQDHQLPDKDCFSDWKPLLNEKRADLAIIATMDRLHAEPALLAMEKGYDIILEKPM
                     ATTLEGCRQIVDAAKETGRMVSVAHVLRYTTFFSKIRGLLDGGRIGRLLQINLTERIG
                     YYHMAHSYVRGNWANSQTTAPIILTKSSHDLDILYWLIGEPCRSVYSEGGLVYFRKQN
                     HPKGAGERCLSCGIERDCPYSAKKIYLELVEDWPIARNILPGASFEEKVRYLETGPYG
                     RCVFLCDNDVADRQTVLLDFGETLAVFNLTAFSAEKTREIDILGTDGDIKGNFERGWI
                     EIRRYGGTTERITIPQEKGHHAGGDYRLLDDVIKRLRNADKDSLSSPEESLHSHEIAF
                     ACETSRREKRPVTLRNGYP"
BASE COUNT         4841 a         5513 c         5066 g         5176 t
ORIGIN      
        1 ctaatcgata tgaaaaacga ggaagagtta aagaagcatt taatgtagag agtcctacaa
       61 aggtcttgaa cacgatttga tgggccgtat gtgggaagca attgaatgta ctcaagaaac
      121 aaggccagaa gcggtcgcac gttttttagt ccgcgtggtc aacgcggcgt aagcgctttg
      181 tacaaagacg gatcgcgaga cttcgtgggc tgggaacacc ctgggaacgc cgcactttag
      241 tgcggcatgt tttcaaggct cttcgacaat ttacgagata tcaataatta acatcttcta
      301 tcgtaataat tctttagacc ttaagaccgt ttaactaatc cgctctaatc gcacaatcgg
      361 ccatctatcg aattatttat tcctcttatg ttactttgta tttctcggat gatcaatggg
      421 cttcaagtaa tgtatcgaag agccgttttc aataaagaga aaaactacag agcggatcgc
      481 ggggtcgtaa aaaccactta ccccgcgata acccacatga gcatcgnnnn nnnnnnnnnn
      541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnggcg taagcgctaa gtacaaagac ggaccgcgag
      661 acttcatagg ctgggaacgc ctgggaacgc cgcactttag tgcggcatgt tgtcaaaaaa
      721 gagaaaaact acagtgcgga tcgcggggtc gtaaaaacca cttaccccgc gataacccac
      781 atgagcatcg cggcgtaagc gctaagtaca aagacggacc gcgagacttc ataggctggg
      841 aacgccaact tttaagttgg catctcttga aaaaaagctc acaccgaggg cacggagggc
      901 acggagggca caaagaagag atgtgttttc gagagtcaac accaggttta tgtttccact
      961 gtgttctctg cgttctctgt gcggaaccat ctttcacttt cctaaaacat gccgaactaa
     1021 agttcggcgt tcccaggcgc accatcgcga cgtaagcgct aattacatag acggatcgcg
     1081 agacttcata ggctgggaac gccaactttt aagttggcat ctcttgaaaa aaagctcaca
     1141 ccgagggcac ggagggcacg gagggcacaa agaagagatg tgttttcgag agtcaacacc
     1201 aggtttatgt ttccactgtg ttctctgcgt tctctgtgcg gaaccatctt ttacttttct
     1261 aaaacatgcc gaactaaagt tcggcgttcc gaggcgcacc atcgcggcgt aagcgcttct
     1321 tctttatgcg acggcgcgat gcgcttttat gtgagctgat cgcgacgttt taaaaaacta
     1381 aaaggcgtat cgtggggtcg taaaaaacca cttaccccgc gataacccac aagaagtcag
     1441 cccgctggtt accccgcgat gaacctctta gttatgtcac cccataactt gacccacact
     1501 cagcgaagcc aagtggtatg gagcacttcc gcattcccag gaaggcgcat tttcaatgcg
     1561 taatgctccg ccgggatgtt tcgcagatag atgagcaatg cgatcaacac gaacccgtgg
     1621 gtgatgaccc aaacgcgttt cccggaatgg atttcaagta tcttttccat ccctgccaag
     1681 gcccgcgaga ggacatcctg aaacccttcc ccattcgggt gtttgaaggt ttgcgggttt
     1741 tgtacgtatt ctttgaagac ctcggcgtct cgctctttca cccaagcggc cgttttccct
     1801 tcccaatagc ctaaactgat ctcgttgagt tccggatacc ggtcgatcga tagcgatagc
     1861 cccgcggctt ccaaagcgat ggcccccgtt tcttgagacc tcaattgcgt cgaagtgcac
     1921 aagacctcag gcgacaactc ccggatcacc agtcccagct ggcgggcttg atcccttcct
     1981 tttaaactga gcgggctgtt caggctgcct tgaaatcggg cttcctcgtt ccatagggta
     2041 gaggcgtgtc gcgtcaggaa gatcgtggtg cgcttcaagg cagcgcttcc gattttatcc
     2101 gttcaaagag tttgtgaaag gcgcgtgagc gatggctaaa gttattcttg atgacgtctg
     2161 tcaactcgcc gaaggtgctg tcaaagcctt tggggatgaa gatgggatcg tagccgaatc
     2221 cattgtctcc ggagatccgt tcggcaatgc ttccttccac caccccttcg gcgctgagcg
     2281 attgcccagt cagcgggtca tagtaggtgg cgaccgttcg aaaacgggcc acgcgctctt
     2341 ccggtttgtc gaaagaggac atacgttcga gaacggcctt catcttctct tcgaaagggc
     2401 ggtcttctaa aaaccgggaa gactgtaccc ccgggaagta gctcaaaatc ggtatctcca
     2461 aaccggagtc gtctgcgatc gagatgattc cgaagtaatg cgcggcggtc ttcgctttca
     2521 gaagagagtt ttcttcaaat gtcgcgccca cttcttcgac ctcccaatga tccgtgaaat
     2581 cgctaatggc gagaaactcg acatctggaa tgatctctgt gatctctcgc accttaaaac
     2641 ggttggtggt cgctagcaat atcttcatag ggggttctct ctctgtgtga cgctttcgtc
     2701 tatctgccct ttgatcttcg ctgaaagctt ctccataatc ttccgacctt cggagctggc
     2761 gttcagttct cctacccacg cagggttttg cgtgagcagg ctcatcacac tcaataggat
     2821 cagctcctcc tgttctccgt tggaatcgtt gagcgcgtgc agtctcacaa ggaagctcaa
     2881 cgcttgccgg tcacggatgt acgaaaggat caggatgata tgatctcgca ggaggtcctt
     2941 ctcctcgtct tcaaacagga gcaattccag aaggctgagt aattcctttc ctccccccag
     3001 cttggcgatg gcttcataga catagagctg gtcgatctct tgctcgaaca gcttgaggta
     3061 ggagtaaagg accgggatgt ctcctctagc gccgatatcg ccaagggtct cgacaaggat
     3121 cagtaatccc ggtaaagagg cgctctcgag cgtttctttc tccaaaaact ccagtatccg
     3181 cggtttagcg gccgatccga gccgaaccag agcttcctgg acgatcgaca ggacttcggc
     3241 atcttcagag ctcttgatga gatccattaa cccgtccaaa gcgtccaagc ctttttcgtt
     3301 gaccaaacgg tctaccactt cctgaggtgt ttgttccatc gtagattctc ccacatcttg
     3361 cgcgatcggt ttcggcgacg acggtcaagc cgatgaacga ttatagcata gaaaaaagag
     3421 aaggctcctt tctcgctaaa acggagcctt cccttctatc gcgtgcagac tcaaggattg
     3481 atggaaaaga agatgggagg actgttggcg ttcaccagtt gaatcaccag catcatagga
     3541 gaaccgtacc gctgaatcca taaaatgatc gaagaattcc acggagcgct cagaatcatc
     3601 gactgcaatt gcgtggagga gtaaacccaa acgccgctcg gcgtctgcgg atagccgtaa
     3661 aaaccgtaag cctgcacata gttgttgtaa accatcactt gcccttgcaa gatttgtacc
     3721 gaccgtatga tatctcctgg gaacagctgg ccataagccg ggtatccggg taacacttct
     3781 gtgactaaca caccgtagcc gttgttgtcg acggtggaga tgcccaacct gatcccacct
     3841 gcgggaggtg gtgtggggta aggatagggg tagggatacg gatacggata tacgggttgt
     3901 ggttgtggtt gcggttgcgg ttgcggggtc acgacgatcg gtccaaaacc gaattctatc
     3961 gtccaccccg caaacagagc cgttgccaat acgagaagcg caagataaac tatagttttc
     4021 tttgccacac aaatccctcc tttttggatt tggcgccttt ttccatccga tacacaccac
     4081 tcagttaaca cgactaagac gtatttatga ctcgatttaa tggatcccat cacgtaccgg
     4141 atcgtcgcga aaccatcgcg catcacactc gatcggggat tggcagatca aagaccccga
     4201 cgactcacgt tgtcttcaaa cagctgcgcg atgtggcggg gatcgatttc gaagagccac
     4261 acttccaatc cttccacgtt cgaaaagccc cgaagcgatc cccattcgaa cctctcgagg
     4321 gcgagtctgt cgatgaaggc gaggcgcacg ttgcgcgagc gtttgagtga ttctatgctt
     4381 ttaataccta aagcgtccgc cagtatccgg attcgcgaat agaggagcag ttcgcgagcg
     4441 ttgggctcta acgggccgaa gcggtcgact agctcgagtt ccacggcctc tatgtctgcg
     4501 gaagttcgag agagggcgat cttcctgtag atcttcatac gctcgatctg actcgcgata
     4561 tagaggtcgg tgagcacaca gttgagcggg aatcctttga tctccacccg gatggtctct
     4621 tcgggctcct cttttgtttt cttttcttct ttcaacccgc gttttaccaa cgcagcgtcc
     4681 agcatctcgc ggtacaagtg aagcccgacg gtgttgatgt tgccgtgttg ctcgaacccc
     4741 aaaagattgc cgatacctcg gatctccata tcccgaagag cgagttgcaa accgcttccc
     4801 gctccggaaa aggtttgtat ggcgtccaac cgagaacggc tttccggaag caggcggcca
     4861 agtttgggat acaaaaagta ggcgtaggcg cgccgatcgc tccgccccac ccttcctctc
     4921 agctggtaca gctgggacaa cccctatcgt tgggcatcgt cgatgaccag ggtgttggcg
     4981 ttcgggatgt cgacgccgct ctcgatgatc gtcgtacaaa ccaggatatc tatctggcct
     5041 tcgtaaaatt cccggatagt ctgttcgaag atcgatttct tcatctgacc gtgggcgagg
     5101 cctacattcg cttccggtac gagcgcccgt acttggtcca gtgattcgga gatatcctcg
     5161 acacggttgt gaacgtagat cacttggccg ccccgcgata gttcccttaa gatcgccgtc
     5221 ttcacgatgc gttcctccca aggggccacc agcacttcaa ccggcaggcg cccgggggga
     5281 agcgtgtcga cgaccgacat ctctttgacg ccggagaagc ccatataaag tgtccgtggg
     5341 atcggagtag cgctgagggt caacacatcg acttccgttt tcttcttttt tatcttttct
     5401 ttctgtgcga ctccgaactt ttgttcttcg tcgatgatca agaggcccaa atctttgtac
     5461 ttaatccgtg aagagaggag gctatgtgta ccgatgatca cgtcggcgga tccgtccgcg
     5521 atcatcgaga gcgccaattt tcgctccttt tctgtgacga agcgattgat cagaatgacc
     5581 ttcgccccat atgctgaaaa ccgttcggaa aaggtctcat aatgctgttt cgaaaggatc
     5641 gttgtcggaa cgagcatcgc cgtttgcttc ccgttgagcg ccattcggaa ggcggcccgc
     5701 atcgcgattt ctgtcttccc cgaccccgca tcgccaaaga tcagccggtc catcggatac
     5761 tctcgggcca aatcgtgata aacgtcttcg atcgagcgtg cttggggctt cgtctccaca
     5821 tacgggaaag acgcggcgaa tttggattcc agctcgaagt ctccgaaaac gggtggcttc
     5881 gtggtggctt tgcgaatcgc gtagagttgg atgagctcct ctaccttttc ttcgatatcc
     5941 tctcgcactt tgttccgggt cctgatccac ccgttgcctt tcagccgtgt caatccgacg
     6001 agtgtctcat ccccgatata ctgatgcacc cgatccaaat tttctacggg cacgaagata
     6061 ttcacctgat ccgcgtattc gatcttcaga tactcgcggg tcccagcgta attgctgatc
     6121 acctcgttcc cttgataacg accgatcccg aagtcttcgt gaacgattaa atcccctttt
     6181 tctaggtctt cggacgaggc gattatttgg cttggatatt ctaggaaagc gctcgtttgg
     6241 ctttttcgct tgacgggagc caaaccgtcc gaagaaacgg tgtagtccgc gaaaagggcg
     6301 gcctgatccc ccaggcttac ggacggacct ctcgattccg tcagaaccgt aacgttctcc
     6361 ctggacagca gggcgctttc cgatacccga aggtacttga ggtatatttc tttgtactcc
     6421 gcttgagggt aaatgtcgaa gagctctttc tcgtaagcgc ggaatcgttc gagggcttga
     6481 gagagccgaa cgtaaaaaaa gaccgtgtcc ttaggcaaga gattgactaa ggcgcgttgt
     6541 gtggggagca acataccgcc aagtgtgggg aatcgatccg gtttttgctc taaggtgccc
     6601 aagaagaggc tgttcgggta tctctttttc aggtccgcga tacgattccg gaaacattcc
     6661 aaattctctt ctttgaagaa ggtctcgcga aagggaggaa tgaggcgttc gccttcctgc
     6721 cgctgacatc cctccgtcga gtctacccct ctcggcaagc tcagctgggt ttgaggatcg
     6781 aagaagcgca cgtcgctgac ctcgtcgtcg aagaattcga tgcgtgttgg aaagtcatct
     6841 agcagcgaaa aaaagtccag gatgaaccca cgcgcggaaa actcgcctag gttttcgacg
     6901 gtatagactc ggcggtatcc gctttccgtg aggtgttgga taacggattc ccgatcatat
     6961 cgtggtctcc cgtcccctgt gatataccct ccctttttga aggtcagaga gcttgtgacc
     7021 agaaggcgcg ggtcgacgat cttttgaagc agtgtcggaa tcgtcgtcag tatcccgccg
     7081 ttgaagcgtt caaagctgcc cagacgatat aaggttttca tccggttttt gagcgtcggt
     7141 atcgaccctt ccacctgttc gaagggcagc agatcgaaag gggggaatag gcaaaggtct
     7201 tggggcgagt ccaggctccc gaagtcttga agcgacttca tatcttcgaa agcgataacc
     7261 cagggcttcg atagggagtg taatcgtgag atcaaccgct tgagaaagtc gtcgtgaagg
     7321 tctgttttca gataagccgt gtgttttccc gttaatggga gcttctctat ggcgcagtcc
     7381 atcttgtttc tcctcgctat cctcgaacga tgggcgcgat atggtatttt tccgtcactt
     7441 catcgaagac cgccaacaca tccaaaatgt atatggaacg acggtcgaaa ctgtacaagg
     7501 tgaaagagag cgtggattcg tggaaggtga gctgcttgat cctgttgatc ggcacatcac
     7561 gaaatatccc gacccatttc aaacggggtt tgtggtctat tcgatcgagg atgaaagaca
     7621 gcgcgttctt gatgttgcta gtggaatagt tctcacgtga gaccgaggac cgtttcatcg
     7681 ggaatcgcgc aataatccga tccgacgcgt tcgtcttgat agacgcgaaa agcccttcgc
     7741 ttctcaaccc tggggcttcc aacggctctt ccgagtaaac ggagattccg cgaacgacgt
     7801 agagggggaa ggagaggatt tgctccatcg atagggaggt gtcttccgga ctctcgtcgt
     7861 agaccctcgt gtcggttccg gcaggcgtga aatccctgag cgcgacctct tttcccggcg
     7921 gttctatcga agaaacagaa gcctcctctg tgaaaacggc tgttttgacg attcggtcga
     7981 agcgtccgac ggtttcctcg ttgaaacctt tgaccacgca tagggcgtcg gagtactcct
     8041 cgagatcggt gatcttcaac tcggttccat aggcgtgaaa gccttctaga gcaatcgcgt
     8101 cggttaacgg cgcatagatg tcgccaatac cggtgtcgaa ttcgaagtcg gttcctaaaa
     8161 cggagatcgc gccggtttct ttttttacgg agtccgccaa gtctttgaaa aagtctttta
     8221 ttttatcgaa caaacgtaat ccagagcctc cttaaggcga gaaaacggtt cgaggatatc
     8281 gatctctttg gagatggtgg tcagccattg gtgttcgtcg gaagttaagt tattgacata
     8341 gacgatcttg tactctttcc cgatcatatc cgaacggtac cgatcatcgg tggcgtccgc
     8401 atccgtcgtt tccttcttaa tagactccaa aaagatgagc gggtacttcg attgggtttg
     8461 tagcaaggtc atatcacgaa ccaggatgaa acggggtttc tcttgaatgc tgatgatcaa
     8521 ctttttggaa agaacgtcca cggtcagata cttctcgatg gacttgccgt acaagatttt
     8581 ttgaaggtta gtcggtttga tcggttccgt atacttaaat tcgacgggga taccgtaata
     8641 gtcggagatg aggatcccgc cgacataggt ttcgtcactc ttcttttcga ttcctaaata
     8701 cccgaatacc gaggcaacat ctgccatact agggctcctt tagctttgaa cagctaggga
     8761 tgttctcaac cgcgcacgtc caagaaatgg ccccgataat cgtcttcggg ctttttgggg
     8821 ggctgtttct cttcttcctt tttctttcgg gatagttctc cttgcgctgc cccttgttct
     8881 tggtcggtag agtgcttgac gccctcctgc tctgcctttt gcgcggcagg ggtcgtgtgc
     8941 gtcgattctt tggttttttt cgacatctcg gaagccaacg cctgttgggc ggcctcttgg
     9001 gtgatcagct gacggctgac gctttgcgcc gcttcggcag gcttgacaaa agaggtttgc
     9061 atctcaagcg gtcccgtcat catgactcct cctttctgtg ccggttaaaa aaactgtctg
     9121 cgctcttccg cttccgcttc ttcgatccta tgttttaacg ttctcaaggc cttactcatt
     9181 atttgcgaaa ccctcgactc ggtaacgccg aacacgaggg cgatttcttt taagttgagc
     9241 tcttcgatat agtaaagcga aatagcgagc tgctctcttt cggtgaggct tgaaataaag
     9301 cttttcaaac ggtcgcgcct ttcgtctttt tcaaactgct gttccggatt caactcctcg
     9361 ctgacgatcg tttcggtaaa atcggtctca tcggggctga ggtagttgtc caaatccaat
     9421 atttgcttgc agttcatatc catacgggtg gtttgtacct cttccaacgg gagtttcagg
     9481 tattcggcga tctcttcttc cgtgggcgtc gctccgttct tcgcctctaa ttcgaagtag
     9541 gctttctcga tttccttaat ctgccggcgc gttttacggg gcaaccaatc gatacttcgc
     9601 aagtaatcgt acattgcccc tttaacccgc gtgagcgcga atgtggtgaa gctcgcgcct
     9661 ttggtttcgt cgtagcgatc gaatgcgttg ataagcgcga cgaccccctc ctgaacgagg
     9721 tccgaaacct ctacgctctt cggcaaggtc gatttcacgt ttgaggccag cgctttaatt
     9781 ttgggcagaa tcatttgtat gagttggtct tttgtgagcc tattttggct gtatgaagaa
     9841 tcgagacgct tgtttttgat cacactgctg cccttcctta tcgtatggaa tcatttagtg
     9901 cccgctctcc gcctacagct ctagaatctc ggtcttttct ccccctccca cttttcgtat
     9961 ctgaaggatc cccgtttgga tattatactc tatactcctg gcgcgggtac cgccggtgtc
    10021 ttcacttaat agacggattt ttttctgggc gagtacgttt ttcacggctt cggtgtttct
    10081 tttccccact tccattgacg atccggtgga tttaaacatc gctgcgccgc cggctatctt
    10141 cgcctcgagt ttacttaagt tcgctcctaa agctcccatc tcttttaaca aaacctcaat
    10201 gccggaatcc gcatatttac caggtttcga gacttctttt ccgccacttt ccgggagcat
    10261 aacgtgaatc agaccgccaa ctcctttggc agggtccctg atgcatactc ccacgcaaga
    10321 gccgaggccc aaggtgacga gaaccagagg gttcttaccc gatttgtact ctccgatgcc
    10381 aatgattgct ttttcttcca tagtgctcac attccaagcg attcaagcaa ttttttcacg
    10441 gattcgggtt tcaatatcaa aaacagaaag gcctccacat tctgctgacc ctcgatctgg
    10501 aagttggtct cgatgagaat cgtgtgatcc tcttcttcgg catactctaa gcacgattcc
    10561 gcgacgatac cggagaccat atcaacggtg atttgaggaa cttcggagtt cacgaacaga
    10621 ttcgtaaact gcgtaagcga cacgagatag gaaccgcaaa cgatgttgcc aatctcgctg
    10681 aaaaccgatc gctgcatttc gtccattgtc gtgaggtctt cgttcacgaa tccgatcaac
    10741 agctccagca accttcgacc gggctcggaa tcaaaaagca ggaggagttt tccattcaac
    10801 tcctcatgaa atcccagctg tatgcctacg gtcacttttt cggggtcttt aaaaaataag
    10861 ggaatccgag aaaactcgat tacagagacg ctggggactt ttatctccac ctttttaccc
    10921 gtcatcatag aaagcgcggt ggccccgttc cccgttccta tgttgccgat ctctttcaac
    10981 acatctaagt gttgaggcga cagttgtgca tagtacgaca tcaaggattc gctccttctt
    11041 ccttaatttt ttttcgatat tcctgttcta aaacgaagac ataccggacg attcggtctt
    11101 cgatcgaacg aggaagatcc ttgaactcca ccccgtagat ccagtgtttc gtttggacat
    11161 tgcgatcgct cctgacaatc tgtgaggtaa ccccgtccaa ggtgatggat tcgcaaacaa
    11221 ccatattcac gaccaccttc tccgaaaccg tcatttcaca gggcgacacc atcagcaatc
    11281 ctccggcgct caagtctttc gtaatgaatt tatgagtctc atttgttttc aaacggatga
    11341 aatcgccctc cacgaagaac gggacccgaa cgaactggcg cctttgtatc ttccgcaggt
    11401 tgtccggaaa aaggatgtac gtcaccaaga atccttcttg atccttctcg taggatatca
    11461 ccatactctg gaaaacgtac atagagcttt tatccaccat cttcacgtat aggcgcgttc
    11521 cggcgggcaa cgggacgaac tgaccttgag agctcggcat cccgattttc atcacctttt
    11581 tgttgaaatc gatgtcatag atgctgctct tgtatacgcc ctccaacaga gggtcggata
    11641 cgtcgatgat cagcggcttc ccaattctta gcccttcgct tgctttgatc tgcgtattga
    11701 agtatgccat cctttcccta cctaatgaga aacacccagt ttcgcgagct tctggtgaaa
    11761 cacgaggagt ttctttttta gttcattctt ttgctcttca tccgccagtc ggagcaagaa
    11821 cccgtactcg aaaaagttgt agttggtgaa ttcataggct ttcttaacgt gaaacggacg
    11881 ttgtatgccg aagatacgga tcgtcccttc ttccgctaac aaacggttct ggcaagagac
    11941 cccaataccg gccatcgaaa cgtctttaac gattacctgc ctcgactctc cccgtgattc
    12001 gaaggttcct ttttctatca aaaagtaacg tttgtaattc ctcttctgag aaaccatcgt
    12061 ttgtcccgga gacaccaata acctttttac tttaccatat acggggtcgt ctttgaccat
    12121 ttgctccttc agtcgtccct cccccttgat gatacccaga gggagaaaga tttgatagac
    12181 gatttcactg tcgtttcgca aatcggcgct gatttggaat acccggcttt cgaacagaag
    12241 gaggtctttg tacgtgccca aaaaaacgca gaataaggag cggcccggta attccagcat
    12301 cgatttccaa cagctgagct tcaacagctt gcttaaattc tggataagga tcatcgtgcc
    12361 cctacttgat cccgaagagc tttttgaact ttacgaaaaa gccattgggc tcttcgtctt
    12421 ccttatactg gcttttgaga atcatcttcg acaagcgcaa tatcgcttga gccggctggg
    12481 aatgcctttg agtttcgatg aaaggagcct gttttaaaac gctagcgctc acgccagaat
    12541 cctccctcac gatcaacttt tcggctatcg tccagcccgc gaatttttct agaacataag
    12601 agaaccgttc gagcacctgc gtgtctttac tcgtgtttcg cgattgattc ataatgaggt
    12661 acagctccgg aaaaatccct ttcgtggcca acaccttcgc cagggtgtaa acgttgatga
    12721 gcgcggtggg ttcaggcgtc gtaaccaaaa acagtttgtc cgctgcctga taaaaagcga
    12781 cgagctcttc gttgaacccg gcgcctgtat ccaccaccgc atagtccata tccacgacga
    12841 gtttcaagaa atcgtccata agtcgcagtt tcgactcgtt tcgaaaagcg atcagatcgc
    12901 ccacatccgt tccgctactc aggagccaaa tgccatagct ggtcgggtat agcacgtctt
    12961 tgagttccat ctttcccacg agatagtcgc gtagggtgtt tttggtcgtt aaccccaata
    13021 gaatatccac attcgcgaaa cccacgtcag cgtcgaaaag caggacgctc ttctgatttt
    13081 tcgccaacgc caccgcaaag ttcgcggcga tcacgctttt cccaaccccg ccttttccgc
    13141 tggcaacgac gatgatttcg aaatttttag ctgctacaac ctttttaagc gtttccaact
    13201 ggtctggcat tagtcagcac ctctttcacg ataaacgaag ccaaccgttt cgagtccgct
    13261 atcatgatat cttctggaat cctctggccg ttcgtgatga agcataccgg aatgtcgtat
    13321 cggctactca gttcaagaaa tgacccgtag acggaggttt catccatctt cgtcagaatc
    13381 agatgggtgg gcgcgatcac cgaaaaatgc tcgataatgc tgtacatatc tttcgagcgc
    13441 gtgttcatgc tcatcgtcag cagcttatac ttcggattga cgatttgcag gtatcggctc
    13501 aattcgttca tctgcagttc gttcttctga ttccttcccg cggtgtccac gagcacgagg
    13561 tcgaagtctt taaaccgttc cagtagcatc gatagttctt tcgggttata agcgacaccc
    13621 acgccgatac ccataatgtc cgcgctggtt ttcagctggt caacggccgc gatccggtag
    13681 gtgtcgatcg ttagaatgcc gattcgtttt cgcatctcga gggacagcat cgcggcgatt
    13741 ttggtcaacg tggtggtctt ccccacgccc gtcgggccga tgaagaacag gatatcccct
    13801 ttttcgtggt tcttgataaa attatcggtt cttatggctg gagataaaac ttccgaaaac
    13861 ttcctttcca cctccatcgt gttgtccatc ccgtcattcg tgacgatgcg caaggactcc
    13921 accattcttc tggacaagtc ctgattaacc tcttggtcga tcagcgcttc atagatcctg
    13981 cgaaaccgct ccggaaggcc tgggagatag tgttctttcc ctatcttgac gctcaagtcc
    14041 tggatcatcc gtttcatcgc caacatatcc ttccgaagcc ccgacacgtc acgggcgccc
    14101 gcgtcatcgc ctccccccca ctccgttgcg ggaactccgg acagtctcgt aaaagaggag
    14161 tcctcatcca ccttttcgat cttgggaagc cccaccgaat acccggttga cggcacgctc
    14221 ggcgtcttag gtcgttcctt cggagaaggt gacgtctccg cttgtgcctg gacatttttc
    14281 gacaggatgc gtttcagttc atagagtttt ttgctgtcga tccattcctc atcttttggc
    14341 ggggtcttcg atttgatttc ttctttcttc tcttcggcga cggcggtcac ctcgtacttg
    14401 gttctgctcc cgaatcccaa aaatccgccg gacttgaact ttcgcgtgcc gagtataacc
    14461 gcgtcttcgc caaggtctct tttaatttgt ttgagcgcgt ccttgacatc ttcgacgacg
    14521 tactttttga ttttcacagg ttaatcaccg cttccaccga aaaagggatg tcctcgacga
    14581 gttcttcgta tgccagaacg ataacgccgg gtaacgccct ttcgatactt cgcttcagat
    14641 agtatcgaat cgacccggaa cagacgacga tcggcataaa tccctttttc atcgctttct
    14701 ccaaggcgtt cgacagagtt ccctccagtt ttttcaacat ctccgggtct atcgccaggt
    14761 accgttcttc cggggtttcg cggatcgaat tcaccaactg ccgttccaaa gacggatcca
    14821 atcccaccgc atgcaaagcc ccgtcgtcgg accgtgcgtc caaaacgatc tggcgcttta
    14881 acccccgtct gacgtattct gtgagataat cgatatcctt cgtcttatcg atgtgctcgg
    14941 tcaacgtttc gaaaatcaag ggcatattgc ggatcgaaac gcgctccttt aatagatttt
    15001 gcaggatccg ctggacttcg aacatcttca acccatccgg tatgacttcg ttgaccaaag
    15061 aagagaactt ttctttcaat ccgtcgatca gcaagctcat ctctcggcgc cccagaatct
    15121 catgggcgta tttcttgatg atctcggaca gatgtgtggc gaaaacgctc ggcgggtcga
    15181 cgaccgtgta cccgctgttg atcgcaaatt cccgttgaga ttcatctatc caataggctt
    15241 ctaagctgaa cgcgggttca cgactggaaa tgcccggcag gatgcccttc gccgtacccg
    15301 ggttgatagc caacaaacgg ttcggccaca attcataccg cgcgatttcc gtgcctttca
    15361 attttatcac atattcgttc gcctttagga gaacgctgtc tcgaacacga ataggagtga
    15421 ggatcaagcc catttcatag gcgagttgct ttcgtacgag ggtgatgcgg tcgagcaggt
    15481 ctcccccttg cgtcgggtca gcgagcggta acaggccata ccccaattcc acatcgatgg
    15541 tgtccgtttg gatgatctcg gagacctccg cggaggtcgt cataaagggc gccggctgtt
    15601 ctcccttcgg acgcggctcc cccggcggcg gcccttttaa acccgcgaat ccttccggtt
    15661 ccggtccacc cgggcctccc gtctcaggcg ccagcttttt cgtttctcga gaggccataa
    15721 accccatcgc gatgagcaac acgccgatca atagggtgga aaatgtgggc aggggcgtga
    15781 acagtgaaag aaaaaccaag actcctcctg ttaggaacat cacgcgattg tccgaggtca
    15841 gctgcttcat catatctcga ctcaggttgt cttccgaggc ggccctcgaa accaagatac
    15901 cgctggacgt cgaaaccaac aaagagggaa tctgggcggc gagcccatct ccgacggtca
    15961 atagggtgaa aacttccgcg gcctctccta tactcaaacc ctgttgaacg gcgccgatga
    16021 tcaatccgcc gatgatgttg acgaagacga tgatgatacc ggcgatggag tctcctcgaa
    16081 cgaatttgct cgccccgtcc attgcgccgt aaaaatctgc ctcgcggcgc acgtcgtttc
    16141 tccgtttttt cgcctcttct tcggtgatca gccccgcgtt atagtcggcg tccacgctca
    16201 tctgctttcc cggcatcgca tccaaggtaa accgcgcggc cacttccgaa atccgctccg
    16261 atcccttggt aatgacgatg aactggatga tcacgaggat taagaaaatg atgataccga
    16321 cgatatagtt gccttgaacg acgaaattcc cgaaggcttt cacgacccgc ccgtcgaaat
    16381 ttttaccctg caagagaatg agacgcgtgg aattgacgtt gagagccagt cgaaacaagg
    16441 tgatcaccag caggatcgaa gggaaggagg acaattccaa cgcgttgcct acgtacatgg
    16501 tcgacaagag gatcacgaga gaaatggtga tattgattat ctgaaagatg tctagcaaaa
    16561 aggtgggtat cgggaggatc atcaacagga cgataccgac gatcagcaac gttatgatga
    16621 tgtcaaagta cttcatactc acacctttcc gaccgttttc aagacgtagg ccaagacttg
    16681 ggcgaccgct tggtagagtt cggcgggtat ttcctccccg atctccgtcg tttcatacaa
    16741 cctcctcgca agcggaggat tttcgtggat cggcacatcg tgctcttttg caatctcgat
    16801 gattcttaag gcgataaaat cgatcccctt ggccaccacc acaggggccg acatcgtatc
    16861 caaatcgaat tggatcgcca ccgcgtagtg ggtcgggttc gttacgacaa cggtggcgga
    16921 gggcacctct tccatcatcc tgcgcttcgc cagttccgcc atcatctgcc gctgtttcgc
    16981 cttgatctgc gggttccctt cgatgttctt gtactcgtcc ttgatctcct gttttgacat
    17041 acggatgctt cgctcgaact cccacctttg gtagaagtag tcgacgaagc ccagaatgac
    17101 caaggcgaaa ccgatccgta tagccagctg atagagagaa tcaccgatca gaaccatcga
    17161 atccatcgtc gaataatccg gcaacaaaaa cagggtattc cataagccgg tgatgaatga
    17221 ccaacctatt atacccagaa tggccaattt tccaatattt tttatcagct ccacgaccga
    17281 tcgtaaagaa aacatccttt tgaacccgtt gatcgggttc agtttgttca agtcgaactt
    17341 gatccctttc aaggtaaaga ggagccgcgt ttgtagcata ccggtcatta gcgcgatcgc
    17401 gaaggcggct aacaagagaa aggctaaaaa gaggaaaaaa tctttgcctt cgataaacag
    17461 gttcaaaaaa gggtctttga aatccgcgat atctccgagg ttcggcaacg atagataaaa
    17521 aacgactaaa cgggtcaggt tttcgtacag ctttgggacg ataaagcgca aaaacacgat
    17581 aatgccgatg aacaccgcgg cggtggccag gtctttcgaa taggtgatct gaccttcctc
    17641 gcgagccttt tggcgacgtc gcggcgtcgc cttttctgtc ttatccgggt ccgcgaagag
    17701 ctgaaggtcg atatacagcc gtatctcttc taaaaccatt tgaagatttg attcatccat
    17761 acggttaact ccctcgtgta acggaccaca acgtctgccc agataggcag aagcccaacc
    17821 aagataatca tgccgacaag aactttcagc ggcagcccca cgatgaacac gttcagctgg
    17881 ggcagtaatt tcgacataat gccgaggacg aaagtgacta aaatcataaa aaagatcatt
    17941 ggaatcccga tttgcaggcc gaaaatcagc acattcccca tcagcgtgga aaactccaac
    18001 ggaaaattcg cgtccattcg cataatcgtc aacggaacca gtcgaaagct ttcgacgaga
    18061 acctggaaca ggatcagcgg ccccttcatc aggacaaaca tgaacaaaaa gcctatcgag
    18121 gtcatctgag agaggatcac gctctctgtc tcagtttgcg gatcgtatat cgttgcggcc
    18181 gaaaaaccga gctggtaccc gtagatatct ccggcgaaat cgacagccca gaacaagata
    18241 ttagcgacca accccgcggc gaacccgaag gtaaaattga gcatttcgat gaggacgata
    18301 tctcccacag gtgtggttag ggggatcggg gtcccaacgg ccggaatcag gatgtacgag
    18361 gtaaaaacga cgaacaacac tttgatcaaa ggagggaagt agtgtccccc aaagaaaggc
    18421 gagtatagga tcaagcccgt gagacggctg atgatcgctg accaggagag gaaataccga
    18481 aaaacgaact gctgtacgtc aaaatccacc gacggatcct ctatatgagg ttcatatagt
    18541 acgtcatcac atcggctata tagtccataa gcttctggaa gatccagccc ccgaaaatga
    18601 ctaaggacaa gaagacgacc aacaccttcg gaacgaacga tatcgtctga tcgttaattt
    18661 gggtgaccgc ttggaagata ccgatcaaca gaccgatagc caaactgaga agcatggtcg
    18721 gagcgatggt gtacagaatc acatagatac cttccataaa aacatccaaa aacgtctcta
    18781 ttgtcatcga acccccccta ccgcctatct gtatattata tcatgaagtt ctcgaccgcc
    18841 gaatccccgc acttctggga acaccctggg aacgccgaac tttgtgggtt atcgcggggt
    18901 aaccagcggg accccgcgat cctatctttg cttttttttg caccgctatg agcccgtaaa
    18961 aatcggtatc gcgccgtcgc gaaaagaaaa ggcgcttacg ccgcgatgcc gcccaggcag
    19021 gatcgccctg ggaacgccgg acttcagtcc ggcatattct ttaagttttt tcactcctcc
    19081 gtgccctctg tgttctctgt gcgaaccctg ctttttctct tcgagccccc tgtgcgaacc
    19141 ctttttgaaa tgcatatcgc gccgtcgcga gaagaaggag cccttacgcc gcgatgtatg
    19201 ttttctccgt gctctctgtg ccctccgtgt gaaatctttt ttcgcacttt ggagaactcg
    19261 cctttctgag aacgtcctag gaacgccgta gtccccccac ttcagtacgg catttacacc
    19321 cccctttcgt gtacaataga catcgaaagg aatagaaggg agtgacccta tgagaccaat
    19381 aacagccgtt ctcgtgggcg cgggaaaccg aggaacccac gcctacggag actacgcgtt
    19441 actcaaaccc gaatccttgc ggatcgtcgc ggtagcggac ccagacggga cacgtcggaa
    19501 gcgcttccag caagaccacc aactacccga taaagactgc ttttccgatt ggaagcccct
    19561 ccttaacgaa aagcgcgcgg atttggcgat catcgccacg atggatagac tgcacgccga
    19621 acccgccctc ctggctatgg agaaggggta cgacatcatc ctcgaaaaac cgatggcgac
    19681 gacgttggag gggtgtcgac agatcgtcga cgccgcgaaa gaaacgggtc gaatggtcag
    19741 cgtcgcgcac gtgctacggt acaccacctt tttctcgaag atcagagggc ttctggacgg
    19801 ggggagaatc ggaagactcc tacagatcaa cctcacggag cgcatcgggt actaccatat
    19861 ggcgcacagc tacgtccggg gaaactgggc gaacagccaa acgactgcac cgatcatcct
    19921 cacgaagagc tctcacgatt tggacatcct ctactggctg atcggagagc cctgccgaag
    19981 tgtctattcc gagggtgggc tcgtctactt ccgcaaacaa aaccatccga aaggcgctgg
    20041 cgagcgctgc ctttcgtgcg gaatcgagag agactgccca tactccgcga aaaagatcta
    20101 cctggaactc gtcgaagatt ggccaatcgc gcggaacata ctgccaggag cttccttcga
    20161 ggaaaaagtg cgatatttgg agactggccc atatggaagg tgcgtcttcc tatgcgataa
    20221 cgacgtggcc gaccgccaga ccgttctcct cgatttcgga gaaacccttg cggtcttcaa
    20281 cctcaccgcg ttttcggcgg aaaaaacccg cgagatcgac atattgggca ctgacgggga
    20341 catcaaaggc aactttgaac ggggctggat cgagatcaga cgctacggtg gaacaacgga
    20401 aaggataacg ataccccaag agaaaggaca ccacgccggc ggagactacc gtttgttaga
    20461 cgacgtcatc aagcgacttc ggaacgcgga caaagacagc ctatcctcgc cggaagaatc
    20521 attacacagc cacgaaatcg ccttcgcctg cgaaacctca cggcgcgaaa aacgcccggt
    20581 aaccttgagg aacggctatc cttaacgaag cggactagcg ccgacggagc gccctgggag
    20641 cgccgggctt cgtggatcat cgcggggtaa gtggttttta cgaccccgcg atccgc
//
LOCUS       DAOPXN010000017        20483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2623_length_20483_cov_5.151704, whole genome shotgun sequence.
ACCESSION   DAOPXN010000017 DAOPXN010000000
VERSION     DAOPXN010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 20483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20483
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2623_length_20483_cov_5.151704"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PKM49_01840"
     CDS             complement(<1..670)
                     /locus_tag="PKM49_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNY81589.1"
                     /translation="MYGSTIFTAIVIVVVFALSSWKLKSTEFSIFLAAVAGAIAGGFG
                     FPVRLLVEGWFTYFDVALIFVTASIFINMYTETGAMDALIRKLVENFFNHKWIMMVFM
                     AILMIIPGALTGAGSVSIFVVGGLVTTVLTYMGLSKGKIVGFIYVFAMLAAAAPPINL
                     WAMLMTAQANMPYVGFDVVLIVPIAIIAAFAIIWFGRGSKPKSKKEVLANLPKPVEKM
                     NWVRI"
     gene            complement(698..1312)
                     /locus_tag="PKM49_01845"
     CDS             complement(698..1312)
                     /locus_tag="PKM49_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNY81590.1"
                     /translation="MRKLGWIVVLVMVLISSSMTFAYTGVDPLNLPVTFLLTNAGQGT
                     GRKMMEAQFLATKTFKKSVDFISNAEPGRDNTFEVDFTDEQGNMVKAVVAAIGSTAKG
                     LGASGITIDDEIARLNKMIEAIKKYNLMLIAIHIEGQVRRGDSANERSIDAIAPFADY
                     IVVTKDSDFDGKFTKLAKEKNIPLTILDNQLEIQKVIRIWYENK"
     gene            complement(1637..2965)
                     /locus_tag="PKM49_01850"
     CDS             complement(1637..2965)
                     /locus_tag="PKM49_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY81591.1"
                     /translation="MKDQLYHDFYIFTGKSPHDYFRGSLWVRGGRIVNVFRSDEDIPN
                     LPWSSKMEGEGKILLPGMINAHTHCYSAFACGLSVHPFRPTSFKEMLEQLWWKLDRAL
                     LSEDVYWSARVSALRSIDHGVTTFIDHHASPYCVRGAGALLSRALIEESGMRAVLSVE
                     TSDRDGAKIRDLEIEENLALMEEKEKHPGKMGALFGLHASFTLEEDTLRRVGSWDFPV
                     HIHIGEGDEDGKLHEEKYGCSAFERLQRYHICKANSLLVHGIRLSHKDIDEIAGTACR
                     VAFNPQSNQNNGAGLPAYARFKARGIPVLLGNDGFGYDFSRDVRSLLITQHHVTQSPL
                     GFSLDDLFQVVFRNNAEYASALLGERVGVIEEGAAADFITYRYKPFTPIDENNFLGHW
                     FFGILENGKPEEVVIDGKALKLGGVLQGPADDAMRTAQSVARKLWERLNN"
     gene            complement(3026..3892)
                     /locus_tag="PKM49_01855"
     CDS             complement(3026..3892)
                     /locus_tag="PKM49_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81592.1"
                     /translation="MIRKAITRVLILLLFLSAGFGAGQIIRNINILGNLNPLLPFSFT
                     YDYTIPSEWQVELFLGTTLNPRQLSAAFSQAATGTFITSGEIGLKFGLPGDVILFAGW
                     PWLGAIFSVQETLWMPELDFSALVPITLGVFSGSAYSESFSFNAVLKKSLRPVRFSGG
                     FNFLTSPQFSSGEFDDEVFEVGQGYLGLGLEWAINPYIRLLGEFRYQFISVLFDTYSD
                     LKFEMFPAYSNQVLGIGFEYILDFPLFLMKATAGYAVKLGLFDPEIGVTYGTHAIHVG
                     VSFLFPFPWRTP"
     gene            4115..5815
                     /locus_tag="PKM49_01860"
     CDS             4115..5815
                     /locus_tag="PKM49_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81593.1"
                     /translation="MKQACVFLCVFLIPIILCGHPLAERITTHWEKAGYKIAYDYPWR
                     SFAFHGRYSHRVQLDLSKNTLTVFTEAFSFSDGFTSLADYASDIRSLFQASGFGQVRL
                     LFSGDGLSDPETGAVYGWLVADASTLPFYLLSELRPETQGFIRLIFDLSYETNRLQKA
                     VDPLANPGGLELPGVKPEELARIAAEDAAILLVQQRQDKALETQLQQIKKELADRSQT
                     ILQLATLNDQLFQRLRTLEETVATYEASETSRIRKEDQIRVEREAFEDLGRQLWEAGL
                     RIKQLESALADKESDILRLNQLLSFREVQTSGLTKEVERQAERIVKLQSENARLTEEN
                     LRLTLTTTPPSEKVLDESDGAEATPTTSPKRVLEERRKVVDNPENDLKIRYSYDVSGK
                     VAVLEVVDAQGAVLETSAYTYDEEGRPLRVRSQGPSSGERTETYDYSERGTVTIEFRE
                     GQTLKAKQRIEKNPEGQTVRVKVYDAKGALNYMEECFHEGGLLKRADGYDASGKRVRQ
                     TFYAYDAENRITRIEIKEGTEVKWVQTFDYATTGEPVQMSLFNSRGVLVEVTEYIYAP
                     "
     gene            complement(6713..8671)
                     /locus_tag="PKM49_01865"
     CDS             complement(6713..8671)
                     /locus_tag="PKM49_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HNY81594.1"
                     /translation="MSGGNESSEFETVSEEQVKAVLAQYDRESNTREFTGIPKYIVRY
                     LLVGFSLYGILANVVFTLPEQIKRASFIGIVIFIAFLLFPASKKIKKRVNRVPWYDLA
                     LGIVGFCAFFYLVVNFRTIADRAGLFTQTDLIWGVIGILIVLEICRRAVGLPIVVVVS
                     CFIAFAFYSGFSFKRLVTHLSYTLEGVIGIPLGVCTTFIVMFIMFGAFLEKTGVGQFF
                     IDVSNAIAGFASGGPAKVAVIASAMEGMVSGSSVSNTVGSGSFTIPMMKKIGYKPEFA
                     AAVEAAASTGGQIMPPIMGAAAFLMAEITGIPYSNIVIAAFLPAVLYFTGIFLMIHFE
                     AKKLGLRGMTKEELPNFWKLLFTKGFLLIPLFVIMYLMMSGSTPTMAAFWSIVSAIAV
                     SMVRKDTRLTPKKAVDALENGARNTLGVAIACSAAGIIIGVVTLTGVGLDLATGLLTL
                     SGGITILALFFTMLACIVLGMGVPTTANYVIMATITAPIVVKLGVPLMAAHMFVFYFG
                     IVADITPPVALAAYAGAAIAHSNPLNTAITATRLAIAAFIIPYMFALNPAILLGPQSF
                     STVASTLQVLFMIITSFVGMVAVSAGMEGYLLKKMRVWERILAIAAGLLLIDPGLATD
                     LIGFSLIALVIVVQYYGSRSKGQPVTAA"
     gene            complement(8854..9354)
                     /locus_tag="PKM49_01870"
     CDS             complement(8854..9354)
                     /locus_tag="PKM49_01870"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HNY81595.1"
                     /translation="MSLFQAHVTVRLILSFARSRLLFFLVLIASIFSLGFARVLIIED
                     AVTAEEIARFDLLEGGSFSIGFVHSVNQSPVEETYEISNGKIWLASCLYYHFGAGVQT
                     EVLPGQEWLTLPDGGMLIENIRLEIGELMYIVGTVSDHVLKIQEKTVSLRELCGRNRK
                     ILVRYQ"
     gene            complement(9363..10307)
                     /locus_tag="PKM49_01875"
     CDS             complement(9363..10307)
                     /locus_tag="PKM49_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNY81596.1"
                     /translation="MKKVAFLFTLALVFSLSLVFGVMNLYMATGGTSGTYYPFGGAVA
                     QVIGQRYPEVRIRVQSTGASAANIRLIAEKEVDIALVQNDVMHYAYTGTVLFKTGKIT
                     NFLTLATVYTEVCQVIAKADGSINSIADLKGKRVSVGDAGSGVEANTIQILEAYGLTF
                     KDISVQNLSFAESAAALKDRKIDAFFVTAGIPTTAIIELSSTNPIKVLSIDPDKADAL
                     IKNYPFYVNFTIPPDTYKNQPEAVNTVAVKATFIVRADLPVDTVYHITKAIFEGQAEI
                     AEAHAKGKEFNLQEAVKGVSVPFHPGAEKYFREVGAIQ"
     gene            complement(10463..11638)
                     /locus_tag="PKM49_01880"
     CDS             complement(10463..11638)
                     /locus_tag="PKM49_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HNY81597.1"
                     /translation="MVVELITVGTEILLGDIVNTNARYLARRIADYGFSCYRQTVVGD
                     NPERLRRAIGNAFESADLVITTGGLGPTDDDLTKEIAAAYFGLPLSFHPQSWERIRER
                     FSTRGMSVPECNRKQAFFPIGAQILENPNGTAPGAMFGNGDKTIVVLPGPPSEMIPMF
                     EAGVVPFLDAKHDGIISSRVLKTVGVGESALAELLKPIIDNQSNPTVATYASEGEITV
                     RVTARAPDKQRALQMIEDTQRSILPLLGDLYYGQDDQSLEGRVIERLIERGWNLAVFE
                     SLTGGALFAALSSVSESTRALSRGWCFNAEEKSRKWLSETQGVNGNGALAFARAAKAR
                     AGSNVGIAVYTQEPQQATIAVSFPEWEVQKTVRWLDTLVKGRRRIVKEALNILRLEG"
     gene            11905..12516
                     /locus_tag="PKM49_01885"
     CDS             11905..12516
                     /locus_tag="PKM49_01885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNY81598.1"
                     /translation="MGEIRPLIFKDHRNREIWIRSAEPEDAKGVIRYTKDQQDRFEYM
                     ISRSWEVNLDVKYEKEYIEAHTQGQNAVMILALHGERVVGLTNFIGGMKQRISHDGEV
                     GISVASDYQGAGIGRCMMEAFLSWASENPTLKRVTLHVMGNNLRAIALYRSLGFVVEG
                     RRRGAVVFETGRVEDLIMMGLILEKGRDLIERNGKKWTDAKES"
     gene            12492..13451
                     /gene="rsgA"
                     /locus_tag="PKM49_01890"
     CDS             12492..13451
                     /gene="rsgA"
                     /locus_tag="PKM49_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNY81599.1"
                     /translation="MDRRQGIVVRFHSNIVTVEDTLTQKRFLCALRGRFKAQNIRPII
                     GDIVEYTGFDDADFERTDDRNARLKGRIENIFPRRNVLDRPNIANIDQVLLVTTLKCP
                     EVSSFIVDRFLVLAERNGLEVCLLINKIDLLDDESRELLHSMVDYYSKDYTVVLVSAK
                     TGEGIREVASVLSGKISTLAGMSGVGKSTVLNQIDPSLGLKEGEISKQLEVGRHTTSY
                     SELLPVANKGLVADTPGFSNLSLTHIPSDELRFCFPEFVRAGCHCYYEDCLHIEEPDC
                     RVHEQVAEGEILPSRYENYLQMYREQADYERARPNLKYQKEKR"
     gene            13448..14101
                     /gene="rpe"
                     /locus_tag="PKM49_01895"
     CDS             13448..14101
                     /gene="rpe"
                     /locus_tag="PKM49_01895"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006262178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNY81600.1"
                     /translation="MKHRIRVSPSILAADFANLERDILRVEEVSDYLHIDVMDGHYVP
                     NITIGIPVVEAIRRVTSLRLDVHLMITRPDDYIDGFIQAGANPLTVHYETCPHLHASI
                     ARIKSAGVEAFVSLNPHTPVSLLEEILPYVDGVLIMSVNPGFGGQRFISGSFEKIRKL
                     ARMREAGENWFLISVDGGVNDDNYRELCDCGTDILVAGSALFRAADPKATILKWKNG"
     gene            14129..16132
                     /gene="ligA"
                     /locus_tag="PKM49_01900"
     CDS             14129..16132
                     /gene="ligA"
                     /locus_tag="PKM49_01900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNY81601.1"
                     /translation="MDLSLDNVKTEIARLREEIERHNYAYHVLASPTISDKDYDELFR
                     RLLALERQYPQYDDPNSPTRKVGAPVLEGFRTVPHSTPMLSLDNTYNETDIREFHGRV
                     VRLLGQSAFTYLCELKIDGVSFAARFEDGLFVLGITRGNGVEGEDITEHIRRIPSFPI
                     RLRHPETLEVRGEIFMPKSAFAQINAQREEEGLALFANPRNAAGGTVRQLDTAVVAKR
                     RLDAFIYHLLDPERYGLKTQAQALDYFRELGFKTEPHRALCASVEDVIAYWRRWTAQR
                     HSLPFDIDGAVIKVNEFGLRDTLGTTTHSPRWSIAFKFPAEQKEARVLSVEYSVGRTG
                     VITPVANLSPVALAGTTVRRASLHNFEYVREKDIRLGDIVVVEKAGDIIPQIVRSVAE
                     KRVGDETTLKPPERCPVCQGPVGKEREEDVAIRCLNPYCSAKLKRSIEIFTSRAGMDI
                     EGLGERWIHELVENGLLKALPDIYRLDRERLIALPRMGEKSVRRLLDEIERSKGNPLH
                     RLLTGLGIPSVGVKTAKELAASFKDLGRLRAATAEELCRLEGIGEEMAGAIRGYFQNP
                     AVQRILDELAEVGVNTKEPIEQASDRFKGLTFVITGTFENYTRDELRNLIEKNGGTVS
                     ESVSKKTSYLLMGENPGSKAQKAALLNTPIMSEAQWQEWFKRT"
     gene            16146..16670
                     /locus_tag="PKM49_01905"
     CDS             16146..16670
                     /locus_tag="PKM49_01905"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNY81602.1"
                     /translation="MKALLVVDVQNDFFSPEGVLFNSRFAERIPEIIRAIGKAQDEEQ
                     YIITTQDWHKSNDPEFRLFPPHCLQNTWGADLLMGLKRSLNGYHRHIRIYKRYFTAFK
                     ETKLDDIFKTNRIQEVSVCGVMLNICILYTCEDLFYREIPTTVLMKASEGPSDEVSKW
                     TYDQLKSVFGVTLA"
     gene            16667..17401
                     /locus_tag="PKM49_01910"
     CDS             16667..17401
                     /locus_tag="PKM49_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HNY81603.1"
                     /translation="MTSRESRFLLIFDLDGTLLDFEKSERISLRKALAKYDRHLDDTQ
                     VAEYRRINAEEWRRFESGSQSKNETTVRRFLRFLAEEGFEELSHLAAEINTEFLTALS
                     ELALYEPYAYETLIRLRQLGYRMGLLTNGVRWVQERRMARSGLKPFFTHIVTSEEVGV
                     GKPHPAMFLSILERFRAKPEDTLYIGDSYESDILGARAVGMATLWYRKGSADDLQTGG
                     VPERVKDDFSDWRTFLGLLEDRENRR"
     gene            17438..18433
                     /locus_tag="PKM49_01915"
     CDS             17438..18433
                     /locus_tag="PKM49_01915"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HNY81604.1"
                     /translation="MKPITASIVLGRGHNPWENLALEEYWMRYPPKENVIFYLWQNQH
                     TIVIGRNQNPWKEVSMEVFEEEGGKLARRLSGGGAVYHDLGNLNFSFIADRTHYDLET
                     QFSVILHTLRGYGLDVRMSGRNDMLLDARKFSGNAYYYLGGKALHHGTILVHADLERA
                     GRYLSPSKEKLNAKGVASVRSRIINLQEKEPDLSIDDLISRLQETFLNLYSPATVSDA
                     TVDVDEGVLREMVGKHASDSWRYGNTPDFEYTRSKRFDWGEVELCFHVSEGRLLRTAV
                     FSDSLYPDFIDRVGAALTDIPFRVPQIVEAIGQVNEPDTALMKTELLMWVKTWDL"
     gene            complement(18503..18814)
                     /locus_tag="PKM49_01920"
     CDS             complement(18503..18814)
                     /locus_tag="PKM49_01920"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNY81605.1"
                     /translation="MKINNSVMAVQNNERILCEKSKKPKDDELKEAVNEFVSILLSQI
                     FKDMDRSIPRTGLTEESYGQSWFREMILDEYAKTASKQALRSLGETVYKQIAGVKQRG
                     S"
     gene            complement(18848..19858)
                     /locus_tag="PKM49_01925"
     CDS             complement(18848..19858)
                     /locus_tag="PKM49_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNY81606.1"
                     /translation="MKKPFACVVVLMLVSLLSFGALVRLKDIARFRGARDNQLFGVGV
                     VIGLNGSGDSGTVPSTLISNMLKNFGISISNQDLKSKNAAIVIVTADIPPYFKPGMRL
                     DIQISSIGDAKSLANGVLLQTPLYGADGVVYAVSQGQISLGGADIKASINLQPRYTVV
                     GTIPGGAIVEKEIPIQIVDAKSVSLLLSNPDITTAARVSQAIDTKFGERISKAMDAST
                     IKVTVPDVFFDDVITFLSIIEEVEVIPDVVAKIVVNERTGTVVMGGGVRVEDFTVAYG
                     GFYVTIRNGKYTAGDNPGVDSSVENLVSALKSIGAKPQDIIGILQAMSQAGVLHAQII
                     VM"
     gene            complement(19874..>20483)
                     /locus_tag="PKM49_01930"
     CDS             complement(19874..>20483)
                     /locus_tag="PKM49_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNY81607.1"
                     /translation="KIGTVALMLLLIAPCFGFSLWSSSNNEKFRNLYSDNKAFKVGDI
                     VTILITETVDLSQSDSTENKSKGLITSTLSIIKNISNVDLFKFLPSSVPDTGSAATAK
                     TNNTAEQQIRAKIAAVITEIDGFGNLTLEGKKEVKVGQDRRELIIQGVVRPGDVSASN
                     TIDSFKIANAKIWYNGDVVFVQDPSEQSWVSYILSGLSKIIF"
BASE COUNT         4976 a         5475 c         5473 g         4559 t
ORIGIN      
        1 ggatcctgac ccaattcatc ttctcgacag gtttgggtaa attggccaag acttccttct
       61 tgctcttcgg tttcgaccca cggccaaacc aaatgatagc gaaggccgcg atgatcgcta
      121 taggaacgat gagcaccacg tcgaacccca cgtacggcat gttcgcctgc gcggtcatca
      181 gcatcgccca aaggttgatc ggaggcgcgg cggccgccag catcgcgaag acgtagatga
      241 agccaacgat cttgcctttg gataggccca tataggtcaa aacggtggtc actaagccgc
      301 cgacgacaaa gatcgacacg ctgcccgcgc cggttaaggc cccggggatg atcatcagga
      361 tggccataaa gaccatcatg atccatttgt gattgaagaa attttctacc aatttgcgta
      421 tcaacgcgtc catcgccccc gtttccgtgt acatgttgat gaaaatggac gcggtgacga
      481 agatcaacgc cacatcgaaa taggtgaacc acccttctac aagcagccga acagggaaac
      541 caaatccgcc tgcaatcgct ccggctacgg ccgccagaaa aatcgagaac tcggtcgatt
      601 taagcttcca gctcgagagg gcgaagacaa cgacgattac tattgctgtg aatattgtgc
      661 ttccatacac agtcgattcc tcccccgatg gaaaaaacta tttgttttcg taccagattc
      721 tgatgacctt ttggatttcc agctgattgt cgagaatagt taacggtatg ttcttctctt
      781 tggcgagctt ggtgaatttg ccgtcgaagt cactgtcttt cgtcacaacg atgtaatctg
      841 caaatggggc aatcgcgtcg atagaacgct cgttcgcgct gtctccgcga cgtacttgac
      901 cctcgatgtg gatcgcgatc aacattaggt tatacttctt gatcgcttca atcatcttgt
      961 tcagacgggc gatctcgtcg tcgatcgtga tgccggacgc gcccaacccc ttagcggtag
     1021 agccgatcgc ggcaacaacg gctttgacca tatttccttg ttcgtcggtg aagtccactt
     1081 cgaaggtgtt atctctcccc ggttcggcgt tggaaatgaa atcgacgctc ttcttgaagg
     1141 tcttggtcgc caagaattgc gcctccatca tcttgcgacc cgtcccttga cccgcgttcg
     1201 tcaacagaaa agtcacgggc aggttcaacg ggtcaacgcc cgtgtacgca aaggtcatgc
     1261 tgctggatat caagaccatt acgagtacca caatccatcc tagttttcgc atactaacct
     1321 ccttataatg ataaaagtga cttttttcca gatcgagcac gacgttattt cgagagaagg
     1381 aacgagtata gtggtcgaag gtatatctgg gccgctattt cataaggggc tcagcgtatt
     1441 cgcggttttt ccgcggaatt ctcccatttt atgttaagaa ccccagtcct gtctagagta
     1501 tcatagttcc gttccactgt caacttatgc cgaacccgat aagcgaaaac aaagcaagac
     1561 taacatcgaa atggtggcga aatcgccggt tttcttcgaa ttcgtctcga ttttcacagt
     1621 ttttaaggta accgaactaa ttgttcaggc gttcccatag ttttcttgcg acgctttggg
     1681 ccgttctcat cgcgtcatcg gccggtcctt gcaaaacgcc gcccagtttc aacgccttcc
     1741 cgtcgattac gacctcttcc ggcttcccgt tttccagaat cccgaagaac cagtggccca
     1801 ggaaattatt ttcatcgatc ggtgtgaatg gtttatagcg ataggtgata aaatcggcgg
     1861 cggcgccttc ctcgatcact cccacccgct cacccaaaag ggcgctggca tactcggcgt
     1921 tattccggaa caccacttga aacaggtcgt ccaaagaaaa gcccaaaggg ctttgcgtaa
     1981 cgtgatgctg ggtgatcaga agagagcgaa cgtctcgcga aaaatcgtac ccgaatccgt
     2041 cgttacctaa aagaaccggt attccacgcg ccttgaagcg cgcgtaggca ggcaacccgg
     2101 ccccgttgtt ctggttcgat tggggattga aggcgacacg acaggccgtg cccgctatct
     2161 catcgatatc cttgtgactg aggcgtattc cgtgaacgag caatgagttc gctttacaga
     2221 tgtggtacct ctgaaggcgt tcgaacgcgg aacatccata tttttcttcg tgcagtttcc
     2281 cgtcctcgtc cccttctccg atatgaatgt ggaccggaaa atcccacgag ccgactcgtc
     2341 gcaacgtgtc ctcttctaac gtgaaagacg cgtgtaaccc gaacaaagcg cccatcttac
     2401 ccgggtgctt ttccttttct tccatcaacg ccaagttttc ttcaatttcg aggtcacgta
     2461 ttttcgcgcc gtcccgatca ctggtttcga ccgataagac cgcgcgcata cccgactcct
     2521 cgattagcgc ccgcgacaac agagcaccag ctccgcgtac gcaataggga ctcgcatgat
     2581 ggtcgatgaa cgtcgtcacc ccgtggtcga tcgagcgtaa cgctgagacc cgcgcgctcc
     2641 aatacacgtc ttcggaaagc aacgcgcgat ccagtttcca ccagagttgc tccaacatct
     2701 ctttgaacga ggtgggacga aatgggtgaa ccgagaggcc acaagcgaag gccgaataac
     2761 agtgggtatg ggcgttgatc attcccggaa gcaagatctt cccctctccc tccatcttcg
     2821 aggaccaagg aaggtttggt atgtcctcat ctgaacggaa aacgttgacg atacgcccgc
     2881 cacgaaccca gaggcttcct cggaagtagt catgggggga tttcccggtg aagatataaa
     2941 aatcgtgata aagctgatcc ttcatcttgt gcctccaaac gagcgcgaat ggccgcggaa
     3001 agatcgggat tcgcgtctat tctctctaag gagtcctcca cggaaaagga aacaaaaagg
     3061 agaccccgac gtggatcgcg tgggtgccgt atgtgacccc gatttccgga tcgaacaatc
     3121 ccagtttgac cgcataaccc gccgtcgcct tcatcaaaaa cagagggaag tccaggatgt
     3181 actcgaaccc gattcccaat acttggttgg aataggcggg gaacatctcg aacttcaagt
     3241 cggagtaggt gtcgaacagg accgaaatga actggtatcg aaactccccc aacaaccgaa
     3301 tatagggatt gatagcccat tccaaaccga ggccgaggta tccctgccca acctcgaaga
     3361 cctcatcgtc aaactcaccg ctcgaaaact gaggggaggt caagaagttg aacccgccgc
     3421 tgaaacgaac gggccgcagg ctttttttca agacggcgtt gaaagaaaag ctttcggaat
     3481 aggcgctacc gctaaaaacg cccaaggtga tgggaaccag cgccgaaaaa tccaattcgg
     3541 gcatccaaag ggtctcttga accgaaaaga tggcccccag ccaaggccat ccggcgaaga
     3601 ggatcacgtc accgggtaac ccgaatttca accctatttc gcccgacgtg atgaaggtgc
     3661 ctgtggccgc ttggctgaaa gcggcagaaa gctgccgcgg attcagcgtc gtgcctaaaa
     3721 acaattccac ctgccattcc gacggaatgg tgtagtcgta agtgaaagag aacgggagta
     3781 gcgggttcaa gttccccaag atgttgatat tgcgtataat ctgccctgca ccgaacccgg
     3841 cgctgagaaa caagagcagt atcagaaccc tcgttatcgc ttttctaatc ataggattat
     3901 tataccccac ccttcttgaa aaaggcgcac acgatcctta ggcccggcgt ataaaagtga
     3961 caccgttttt ttccggggtc ctctttgttt ttctatgggc tgcgcgacct ttccttcatt
     4021 cgaggaggct tgagcgcccg ataggaagaa agcgcgtttc cttagaataa tgaatcgtct
     4081 acgggtaact aaacctcgct taagaaggtg ttttatgaaa caagcctgcg tttttttgtg
     4141 tgtgttcctg atcccgatca tcctttgcgg gcatcccctt gccgagcgta taacgaccca
     4201 ttgggagaag gcgggatata agatagccta cgactatcct tggaggagtt ttgcctttca
     4261 cggccgctac tcccatcgag tccagctgga cctatccaag aacaccctca cggtttttac
     4321 cgaagcgttt tcgttctcgg acggctttac aagcctcgcc gattacgcct cggacatacg
     4381 ctctctgttc caagcttccg gttttggcca ggtaaggctg ttgttttcgg gtgacgggtt
     4441 gagcgatcct gagaccggag cggtctacgg ttggttggtc gccgacgcct ccacgctccc
     4501 gttctatctt ctatctgaat tgcgacctga aacgcagggt tttatccgtc tgatcttcga
     4561 cctatcttat gagacgaatc gcctccagaa ggcagtcgat ccgttggcca acccgggagg
     4621 tctcgaacta ccgggcgtaa agcctgaaga gctcgcgagg atagcggcgg aggacgccgc
     4681 tatccttctg gtgcaacagc gtcaagataa ggccttagaa actcaactgc aacaaattaa
     4741 aaaggagctg gccgatcgat cccagacgat tttacagttg gccacgctga acgaccaact
     4801 tttccaacgg cttcggaccc ttgaagagac agtcgcgacc tatgaggctt ccgaaacctc
     4861 gaggattcgc aaggaagatc aaattcgcgt tgagagagaa gccttcgaag acctagggag
     4921 gcagttgtgg gaggccggcc tgcgtatcaa acaattagaa tcggcgcttg cggataagga
     4981 atccgatatt ctccgactga atcagctgtt gtcctttcgg gaggtccaaa cctcggggct
     5041 gactaaagaa gtggaaagac aggccgaacg gatcgtgaag ttacagagcg agaacgcccg
     5101 attgaccgag gagaacctgc gtttgacgct cacaacgacc ccgccttcgg aaaaggtatt
     5161 agacgagagc gatggcgccg aagccactcc gacgaccagc ccgaaacgag tgctggaaga
     5221 gcgtcgcaaa gtagtagaca acccggaaaa cgacctgaag atacgttact cttatgacgt
     5281 atcggggaag gtagccgtgt tggaggtcgt cgacgcgcag ggagcggtgt tggagacttc
     5341 agcctacacg tacgacgaag agggccggcc ccttcgggtg cggtcccaag gtccatcgag
     5401 cggggagagg acggagacgt atgactacag cgaacgagga acggtcacga tcgagtttcg
     5461 cgaagggcag acgctaaaag ccaaacagag gatagaaaag aaccccgaag gtcaaacggt
     5521 acgtgtgaag gtgtacgacg caaaaggcgc attaaactat atggaagagt gtttccacga
     5581 aggcgggctg ttgaagcgcg cggacgggta tgacgcctcg ggtaaacgtg tccggcaaac
     5641 cttctacgct tacgatgcgg aaaaccgtat cacgcgaatc gagatcaaag aaggaactga
     5701 agtcaaatgg gtgcagacct tcgattacgc gacaaccggc gaaccggtac agatgagcct
     5761 tttcaactcg agaggggttt tggtcgaggt gaccgagtac atttatgcgc cctaataagg
     5821 aaaggcatcg ctgtggttca agttttgggg tgcataacta aaatgttcat cgcggggtaa
     5881 ccaaagggct gacttcggaa gcccacgaag tctcgcgata cgcatctttg actttctcag
     5941 aagcgtatcg cgccgtcgcg aaaagaaggg gcgcttacgc cgcgatggcc gcgtggggtt
     6001 atagtggggt aaccagcggg cagacttcgg aaacccacga agtctcgcga tactaacgaa
     6061 atccagcgat gcgctctctc ctgtctttta ttttagagac aggccaacta aaaatcggcg
     6121 tgcccagggc acccccaagc gtatctcggc gtcgcgaaaa aaggggcgct tacgccgcga
     6181 tgcttgctct ctctgtgctc tctgtgtcct ctgtgcgaac cctgttttac aagacaggga
     6241 caaaagcgta tcgcgccgtc gcgaaaagaa ggggctctta cgcctcgatg cttgctctct
     6301 ctgtgctctc tgtgtcctca gtgcgaactc tgttttacaa gacaggtacg aaagcgtatc
     6361 gcgccgtcgc gaaatgaaga agcgcttacg ccgcgatgct tgctctctct gtgctctctg
     6421 tgtcctctgt gcgaaccctg tttttcaaaa caagcacaaa agcgtatcac gccgtcgcga
     6481 aaagaagggg cgcttacgcc ccgatccttg ctctctctgt gctctctgtg ccctctgtgc
     6541 gaaccctgtt tttcaaaaca agcacaaaag tgtatcgcgc cgtcgcgaaa agaaggggtg
     6601 cttacgccgc gatgctcgcg tgggttatcg cggggtaacc agcgggctga cttcggaagc
     6661 ccacgaagtc tcgcgatacg ccccaagggc attctttttc gtgactcgtt tattaggcag
     6721 ccgtcaccgg ttggcctttc gaacgagagc cgtagtactg cacgacgata accaaggcga
     6781 taagagaaaa accgatcaag tcggtcgcca gccccggatc gatcagtagc aagcccgccg
     6841 cgatcgccaa gatccgttcc caaacccgca tttttttgag caggtagcct tccatccccg
     6901 ccgacaccgc gaccatcccc acgaaggacg tgatgatcat aaacaacact tgcagggtcg
     6961 aagcgaccgt cgaaaaggat tgcggtccca gaagaatcgc ggggttcaac gcaaacatat
     7021 aggggataat gaaagccgcg atcgctaacc gtgtcgccgt aatagcggta ttcaaaggat
     7081 tggaatgggc gatagcggca cccgcgtagg cggctaacgc gaccggtggc gtaatatctg
     7141 caacaattcc gaaatagaag acgaacatat gtgcggccat cagcggaacc cccaatttca
     7201 cgacgatggg agcggtgatc gtggccataa tgacgtagtt ggcggtggtg ggaacaccca
     7261 ttccgagcac gatacaggcg agcatggtga agaacagcgc cagaattgtg atcccgccag
     7321 atagggttaa gaggcctgtc gcgagatcga gtcccacgcc ggtcaaggtc acgacaccga
     7381 tgatgattcc ggccgcggaa caagcgatcg cgacaccgag ggtattacgc gccccgttct
     7441 ctaaagcatc caccgctttt ttgggggtta agcgggtgtc ttttcgaacc atactgaccg
     7501 cgatcgcgga aacgatcgac caaaacgccg ccatggtagg agtggagccg gacatcatca
     7561 aatacataat gacaaacagc gggatgagta aaaatccttt ggtgaagagg agtttccaga
     7621 aatttggcag ctcttccttg gtcattcctc tcagacccaa ctttttcgct tcgaaatgta
     7681 tcatcagaaa tatcccagtg aagtagagaa cagcgggtaa aaaagctgca atgacgatat
     7741 ttgagtatgg gatacccgtg atctccgcca tcaggaacgc tgcggctccc ataattgggg
     7801 gcataatctg tccgccggta gaggctgccg cttcgaccgc tgctgcgaat tcgggtttat
     7861 aaccgatttt tttcatcatc ggaatggtga aactccccga gcccacggtg ttagataccg
     7921 agctccccga aaccatccct tccatcgcgc tggcgatgac ggccactttc gccgggcccc
     7981 cactcgcaaa ccccgcgatg gcgttggata cgtcgataaa gaactgaccg actccagtct
     8041 tttccaagaa ggcgccgaac ataatgaaca taacgatgaa ggtcgtacaa actcctaacg
     8101 gtatcccgat gaccccttcc aaagtgtagg aaagatgggt aacgagccgt ttaaaagaaa
     8161 aacccgaata aaaagcgaaa gcgatgaagc acgaaacaac gacgacgata ggcaacccca
     8221 cggcccgcct acagatttcc aaaacgatca ggatgcctat cacgccccaa atcaagtccg
     8281 tctgtgtgaa caaacccgcg cgatcggcta tcgtgcggaa gttgacgacc aggtagaaaa
     8341 aggcgcagaa ccccacgata ccgagggcta agtcgtacca gggcacacga ttcacgcgtt
     8401 ttttaatttt cttcgatgcg gggaacagca gaaatgcgat aaagatgacg atcccgatga
     8461 agctcgcccg tttgatttgt tctgggagag tgaagacgac gttcgcaaga ataccgtata
     8521 gtgaaaaccc tacgagcaga tatcgaacga tgtatttcgg aataccggta aactctctcg
     8581 tattcgattc tcggtcgtat tgggccagaa ccgctttcac ttgttcttcg cttaccgttt
     8641 caaattccga gctctcgttc cccccgctca tcgccacctg tttatcttcg agagatttct
     8701 tcatttcggc gtcttttcga cccttctttt tctcttccgc cacgagtaac acctccggat
     8761 ataccgctag gccgaaccgc acgactcctt cgtttcttgg acgcccgatc cggtaagcga
     8821 gatgttctta aagcttcgta tataataaat tttttactga tatcgaacga gaatctttct
     8881 gtttctaccg cacaattctc tcaaactgac tgtcttttct tggatcttca ggacgtggtc
     8941 gctcaccgtc ccaacgatgt acatcagttc gccgatctcc agccgtatgt tttcgatcag
     9001 catcccgccg tcgggaagtg tgagccactc ttgcccgggc agaacctccg tttggactcc
     9061 cgccccgaag tggtaataaa gacacgacgc gagccatatt tttccattag aaatctcata
     9121 ggtctcttcg actgggcttt gattcacgga gtggacgaag ccgatcgaaa aagacccccc
     9181 ttcgagtaag tcaaaacggg cgatctcctc agccgtcacc gcgtcttcga tgattaatac
     9241 tcgtgcgaaa ccgagtgaga aaatcgacgc gatcagaacg aggaaaaaga gtagacgcga
     9301 ccgggcaaaa gaaagaatca ggcgcacggt gacgtgcgcc tggaataaac tcatttgacg
     9361 gactattgga tcgctccaac ttcacggaaa tacttttcgg ctccggggtg gaagggaacg
     9421 gagacccctt tgacggcctc ttggaggttg aactcctttc ctttcgcatg ggcttccgcg
     9481 atctccgcct gtccttcgaa tatggctttg gtgatatgat acacggtgtc gaccggaagg
     9541 tcggcgcgaa cgataaaggt cgctttcacc gcgacggtgt tcaccgcttc cggttggttc
     9601 ttgtacgtgt cgggaggaat ggtgaagttc acatagaagg ggtagttctt gatcagcgca
     9661 tcggctttgt ccgggtcgat agacagcact ttaatcgggt tggtgctgga gagttcgatg
     9721 atcgcggtcg tcggaattcc cgctgtgacg aaaaacgcgt cgatcttgcg gtctttgagc
     9781 gccgcggcag attcggcaaa agacaggttc tgaacgctga tgtctttgaa ggtcaaccca
     9841 taggcttcga ggatttggat ggtattcgcc tcgactccgc ttccggcatc tccaaccgac
     9901 actctctttc ccttcaggtc agcaatggag ttgatgctgc cgtccgcttt ggcgatcact
     9961 tgacaaacct ccgtgtagac cgtcgccaga gttaaaaagt tcgtgatctt gccggttttg
    10021 aagaggacgg tgccggtata ggcgtagtgc atcacgtcgt tttggaccag cgcgatgtcc
    10081 acttctttct cggcgatcaa tcgaatgttc gcagccgaag cgcctgtcga ttggacgcgg
    10141 atacgcactt cagggtagcg ctggccgatc acttgcgcaa cggctccccc aaaaggatag
    10201 taggttccag aagttccgcc tgtggccatg tacagattca taacgccaaa aacgagggat
    10261 aaagagaata cgagcgccag ggtgaatagg aaagcgactt ttttcacgta gatctcctcc
    10321 ttcaataagt atgttgccgg taataaaaaa aaccgtaata gggtgcgcga aatagcaaaa
    10381 caagcgatgg gaagaacgat aaacatagtg taacacatat tctcggagat tgcaagagga
    10441 aaaaacgcga aaaccgggcg atctaccctt ccaaacgcag gatattcagg gcctccttca
    10501 cgatccggcg acgccctttg acgagcgtat caagccaacg aacggttttc tgaacctccc
    10561 attccggaaa agatactgca atcgtcgcct gttgcggctc ttgtgtgtac acggcgattc
    10621 ctacgttaga acccgcgcga gccttcgccg ctctggcgaa ggcgagcgcc ccgttaccgt
    10681 tgaccccttg cgtttccgac agccatttcc gacttttttc ctccgcgttg aaacaccagc
    10741 cgcgcgagag cgcccgcgtc gattcgctca cactcgatag cgccgcaaac agggcacccc
    10801 ctgtgagcga ctcaaagacc gcgaggttcc atccccgctc gatcagacgt tctataaccc
    10861 gtccctccaa ggactggtcg tcctgaccgt aataaaggtc tcccaatagg gggaggatcg
    10921 atcgttgcgt atcctcgatc atctgcagag cgcgttgttt atcgggggcg cgcgccgtca
    10981 ccctgaccgt gatctcccct tcggaggcgt aagtggcgac ggtcgggttg ctttggttgt
    11041 cgataatcgg tttcaaaagt tctgccagcg cgctctctcc gacgccaacg gttttaagga
    11101 ccctcgatga gatgatgccg tcgtgcttgg cgtccagaaa gggcacaaca cccgcttcaa
    11161 acatcgggat catctccgag ggcggaccgg gtagaaccac gatcgtttta tccccgttgc
    11221 cgaacatcgc tccaggcgcc gtgccgttcg ggttttccaa tatctgggcg ccgatcggga
    11281 aaaaggcttg ctttctatta cattccggaa cggacatccc tctggttgaa aagcgttccc
    11341 gtatccgttc ccaggactgc gggtgaaagg acaagggaag gccgaaatac gcggccgcga
    11401 tctctttggt cagatcgtcg tcggtcggac ccaatccccc agtggtgatg acaaggtcgg
    11461 cagactcgaa ggcattgccg atcgctctcc tcaaacgttc gggattgtcc ccgacaaccg
    11521 tttgacggta acagctgaac ccgtaatcgg ctatcctccg ggcgaggtat cgggcgtttg
    11581 tattcacgat gtctcctaat aaaatctccg tgcccaccgt aatcagttcc actaccatac
    11641 ttcggccctc ccaatcgggt caatcactaa atacgctgtt tctaagtcga aaggcgccat
    11701 aagaaaacct catacatcga ggaaaaaacg acctctgcag ctccttttgg agtaccctat
    11761 cgagcattcc tcaccaccat acgtcttcgc gcaagaattc tcccgcgtta gggggcatac
    11821 tcccatctct tttacccgcg atcgcacaat tatagcatgg acggccatgg tataattccg
    11881 acatccgata cgaaaaggag taaaatggga gagatacgtc cgttaatttt taaggaccac
    11941 cggaaccggg agatatggat caggagcgcg gagcctgaag acgcgaaagg ggttatccgt
    12001 tacacaaaag accaacagga tcgtttcgaa tacatgatct ctcgctcctg ggaagtgaat
    12061 ctggacgtga aatatgaaaa ggaatacatc gaggcgcaca cccagggaca aaacgcggtg
    12121 atgatcctcg ccttacacgg cgagcgcgtg gtcggtctaa ccaacttcat cggagggatg
    12181 aaacagcgca tcagccacga cggagaagtc gggatatcgg tcgcttccga ttatcagggg
    12241 gcgggtatcg ggcgctgcat gatggaagcc tttctctcgt gggcttccga gaaccctacg
    12301 ttgaagagag tgaccttgca cgtgatgggg aacaacctcc gggccatagc cctctatcgg
    12361 tctttgggtt tcgtggtcga gggccggcga agaggcgccg tggtcttcga gaccgggcgg
    12421 gtcgaggatc tcatcatgat ggggttgatt ttggagaagg gtcgcgacct catcgaacga
    12481 aacggaaaaa aatggacaga cgccaaggaa tcgtagttcg tttccactca aatatcgtca
    12541 cggtcgaaga caccctcacc cagaaacggt tcttgtgcgc tttgcgcgga cgtttcaaag
    12601 cccaaaacat ccgcccgatc atcggagata tcgtggaata cacgggcttc gacgatgcgg
    12661 acttcgaaag gacggacgat cgaaatgccc gtctgaaagg aagaatcgag aatatcttcc
    12721 ctcgacggaa cgtcctggac cggccgaata tcgccaacat cgatcaggtc ctgttggtca
    12781 cgaccttgaa atgccccgag gtctcatcct ttatcgtaga ccgttttctg gtcttggccg
    12841 agcgaaacgg tctcgaagtg tgccttttga tcaacaagat cgatctgctg gacgacgaga
    12901 gccgggagct actccactct atggtagatt attattccaa agactacacg gttgtcttgg
    12961 tctctgcgaa aaccggcgaa gggatccggg aagtcgcttc cgtcctctcg ggaaaaatct
    13021 ccacgctggc gggaatgtcc ggcgtgggca agagtacagt gctcaatcag atcgaccctt
    13081 ccttggggtt gaaagagggt gaaatctcca aacagcttga ggtgggaagg cacacgacgt
    13141 cgtattcgga gctcctgccg gtcgcgaaca aagggttggt ggccgatacc cccggattct
    13201 cgaacctcag cctgacgcac atcccttccg atgagttgag attctgtttc cctgagttcg
    13261 tcagggcagg ctgccactgt tattatgaag attgcttaca tatagaagag ccagactgcc
    13321 gggttcacga gcaggtggcc gaaggggaga tcctaccctc acgatacgaa aactatctgc
    13381 agatgtatcg cgagcaggcc gattacgagc gcgcgcgccc gaacctcaag taccaaaagg
    13441 agaagagatg aaacatcgca tacgcgtctc accatcgatc ttagcagcgg atttcgccaa
    13501 tttggagaga gatatcctac gcgtcgaaga ggtcagcgac tatctacaca tcgacgtgat
    13561 ggacggccat tacgtgccga atatcactat cgggatcccc gtggtggaag cgattcgacg
    13621 ggtcacctcc cttaggttgg atgttcacct gatgatcaca cggccggacg actatatcga
    13681 cggcttcatc caagccgggg cgaatccgct gaccgtacac tatgaaacct gtccacacct
    13741 ccacgcgtcc atcgcccgta taaaaagcgc cggggtggag gcgtttgttt cattgaatcc
    13801 gcacaccccg gtttctctcc tcgaagagat cctaccttac gtggacgggg ttttgattat
    13861 gagcgtgaat ccgggattcg gaggccagcg attcatttcc ggaagcttcg agaagatacg
    13921 aaaattggcg cgtatgcgtg aagccggaga gaattggttt ctcatctccg tggacggggg
    13981 cgtgaacgat gacaactacc gggagctatg cgactgcgga accgatatac tggtagcggg
    14041 gtcggccctg ttccgcgccg cagacccgaa agccacgatc ctgaagtgga aaaacgggta
    14101 aggacaccca agcgcaccgg aggatgatgt ggacctttca ctagataacg taaaaaccga
    14161 gatcgctcgt ttgagagaag agatcgagcg gcacaactac gcctatcacg tactggccag
    14221 tccgaccatc tcggacaagg attatgacga gctgtttcga cgactgctcg cccttgagcg
    14281 tcagtatccc caatacgatg acccgaactc cccgacgcgc aaagtgggcg cgccggtgct
    14341 cgagggtttc cggaccgttc cccactcgac gcctatgctc agtctcgaca acacttacaa
    14401 tgagacggat atacgggaat tccacggtag ggtcgtcagg ttattaggtc agagtgcgtt
    14461 cacctatctg tgtgagctca aaatcgacgg cgtttctttc gccgcccgct ttgaggacgg
    14521 cctattcgtt ctcggcatca ccagagggaa cggggtggaa ggagaggaca ttacggaaca
    14581 tatccgacgg attccttctt tccccatccg tttgcgacac ccggaaaccc tggaggtccg
    14641 cggagagatc tttatgccga agtcggcgtt cgcgcagatc aacgcgcaac gcgaggaaga
    14701 ggggctcgcg ttgttcgcca atccccgaaa cgccgcgggc ggcacggtac gacagctcga
    14761 caccgccgtg gtggctaaaa gaaggttgga cgcgttcatc taccacctgt tggatcccga
    14821 gcgttacgga ttgaaaaccc aagcccaggc gctggactat tttagagagc tcggcttcaa
    14881 aacggaacct catcgcgccc tatgcgcgtc ggtcgaagac gtgatcgcct attggcgacg
    14941 ttggaccgcc caacgacact ccctgccgtt cgacatcgac ggcgccgtga tcaaagtcaa
    15001 cgaattcggc ttgcgcgata cgttgggaac aacgacccat tcgccccggt ggtctatcgc
    15061 gttcaaattt ccggcggaac agaaggaggc tcgcgtcctc tcagtagagt acagcgtcgg
    15121 tcggacgggt gttatcacac cggtcgcgaa cctgtctccc gtggcgttgg cagggacgac
    15181 ggtccgacgc gcctcgcttc acaattttga atacgtgaga gaaaaggaca tccggttggg
    15241 cgatatcgtg gtcgtcgaaa aggccgggga catcatcccg cagatcgtac gctcagtcgc
    15301 agagaagcgt gtcggagatg aaacgacact caaaccgcct gagcgctgcc ccgtatgcca
    15361 gggtccggtc ggaaaagaac gggaagagga tgtcgcgatc cgctgtttaa acccctattg
    15421 ctcggcgaag ttgaaacgct cgatcgaaat ctttacctcg agagcgggta tggacatcga
    15481 ggggctcgga gaacgttgga ttcacgaatt agtggaaaac gggctgctca aagctctccc
    15541 agacatctat cgattggata gagaacggct tatcgccttg ccgaggatgg gcgagaagtc
    15601 cgtccgtcgc cttcttgatg agatagagag gagcaaaggc aacccgttac atcgtttgct
    15661 gacgggtttg ggcattccct cggtcggggt gaaaacagcc aaagagctgg ccgcgtcctt
    15721 caaagacttg ggaaggttga gggccgcgac ggccgaggag ctgtgtcggt tggagggcat
    15781 aggagaagag atggcaggcg cgattcgcgg ctatttccaa aaccccgccg tccaacggat
    15841 tctggacgaa ctcgccgagg tgggcgtaaa cacgaaagaa ccgatcgaac aagcctccga
    15901 tcggttcaaa gggctgacct tcgtcatcac cggaaccttc gagaactaca cgcgagatga
    15961 gctccggaat ctgatagaaa agaacggagg aactgtttca gaaagcgttt ccaaaaaaac
    16021 gagctatctg ttgatgggag aaaacccggg gtcgaaagct cagaaagcgg cgctgctcaa
    16081 tacgccgatt atgagcgaag cgcaatggca ggaatggttc aagcgaacgt aataaggaga
    16141 gtgtgatgaa agcgcttttg gttgtggatg ttcagaacga tttcttctct cccgaagggg
    16201 tcttgttcaa cagccgtttc gcggaacgga taccggagat cattcgggct atcggaaagg
    16261 cgcaggacga agagcagtat attatcacca cccaagactg gcataaatcc aatgacccgg
    16321 aatttcgact ctttcctccg cattgcctgc aaaacacgtg gggggccgac ctcctgatgg
    16381 gtctaaagag gtccctgaac gggtatcacc gccatattcg catctataaa cgctacttca
    16441 cagcctttaa ggaaaccaaa ttggacgata ttttcaagac caaccggatc caagaggtct
    16501 ccgtgtgcgg tgtgatgctc aacatctgta tcctctacac ctgtgaggac cttttctacc
    16561 gagagatacc gaccaccgtc ctgatgaagg catcggaggg tccttcggac gaagtgtcca
    16621 agtggaccta cgatcaattg aaatccgtct ttggtgtgac gttggcgtga cctcgcgcga
    16681 aagccgtttt ctcctcattt tcgacctcga cgggacctta ctcgacttcg agaaaagcga
    16741 acggatttcg ttgagaaaag cgctcgcgaa gtacgaccgc catttggacg atacgcaggt
    16801 ggccgagtat agacggatta acgctgaaga gtggcgccga ttcgagtcgg gaagccagtc
    16861 caaaaatgaa acgacggtac gcagattcct acggttcttg gcagaggagg gcttcgagga
    16921 gctctctcat ttagcggccg agatcaacac agaattcctg acggcgcttt ccgagctcgc
    16981 cctgtacgag ccctacgcct acgaaaccct gatacgactg cgacagctcg gttaccgtat
    17041 gggtttgctc acaaacggcg ttcgctgggt tcaggaacgg aggatggcgc gctcgggcct
    17101 caagcccttc ttcacgcata tcgtgacctc cgaggaggtc ggtgtgggaa agccccaccc
    17161 ggcgatgttc ttatcgatac tggagcgttt ccgagccaag ccggaagaca ccctgtacat
    17221 cggcgactcc tatgaaagcg atattctggg cgcacgcgcc gtcggtatgg cgacgctgtg
    17281 gtatcggaag ggctcggccg acgacctcca aacggggggc gtcccggaaa gggtaaaaga
    17341 cgatttttcc gattggcgca cgtttttggg attattggaa gacagagaga acaggagata
    17401 gaatccgtgg tcaggaccac gggagaacgg aggatcgatg aaacccatta ctgccagtat
    17461 cgtgttaggt aggggtcata acccctggga gaacctcgcg ctcgaagagt attggatgcg
    17521 atacccaccg aaggagaacg tgatctttta cctttggcaa aatcagcaca ccatcgtgat
    17581 cggaaggaat cagaaccctt ggaaagaggt gtcgatggag gttttcgagg aagaaggagg
    17641 aaagttggcc cggcgactgt ccggcggtgg ggccgtctat cacgatcttg ggaacctcaa
    17701 tttttccttt atcgccgata gaacccacta tgatctcgaa acacagtttt cggtcatcct
    17761 gcacaccctg aggggctacg gtctcgatgt ccgaatgagc gggcgaaatg acatgttgct
    17821 cgatgcgcgc aagttttccg gaaacgcgta ctattacctt ggaggaaagg cgctccatca
    17881 cggaacgatc ttggttcacg cggatttgga acgcgcaggg cgctatttga gcccctcgaa
    17941 agagaagctg aacgcgaagg gagtcgcttc cgtccgctcc cgcatcatca atctgcagga
    18001 aaaagaacct gaccttagta tagacgacct catctctagg cttcaggaga ccttcttaaa
    18061 cctctactcg ccggccaccg tttctgatgc tacagtcgat gtcgacgagg gagtactgcg
    18121 ggaaatggtc ggaaagcacg cttcggattc gtggcggtac gggaacacgc ccgattttga
    18181 atacacccgg tccaaacggt tcgattgggg agaggtggag ctctgtttcc acgtgagtga
    18241 ggggaggttg ctccggacag cggtcttttc ggatagcttg taccctgatt tcatcgaccg
    18301 ggtgggagcg gcgctgacgg atattccctt tcgggtccca cagatcgtgg aagccatagg
    18361 ccaggtgaac gaacccgaca ccgccttgat gaagaccgaa ttgttgatgt gggtaaagac
    18421 gtgggactta taataaaaaa ggggggtgcc gcagcctacc tcacaaaacc ccccaacaga
    18481 acaatgaacc cctcgcggca ctttaagacc ctctttgttt caccccagct atctgcttgt
    18541 aaacggtctc tcctaatgaa cgtaatgctt gcttagaagc ggtttttgcg tattcgtcca
    18601 agatcatctc tcgaaaccag ctctgtccat atgactcttc ggtaaggccg gttctcggta
    18661 tcgatcgatc catatccttg aaaatctgtg ataacaggat cgacacaaat tcgttcacgg
    18721 cctctttcag ttcatcgtcc ttgggttttt tcgatttctc gcatagtata cgctcattat
    18781 tttgtactgc cattacagaa ttattaattt tcataacgat tcctttcaat tccgacgagc
    18841 ggacccctta catcacaata atttgcgcgt gtagcacgcc cgcttggctc atcgcctgca
    18901 agataccgat gatatcctga ggtttcgcgc cgatagattt caacgcgctg accaggtttt
    18961 ctacggagct gtccactccg gggttgtctc cggcggtata cttcccgttt cgtatcgtca
    19021 cataaaaccc tccgtaggcc acggtgaaat cttcgacacg gacgccgcct cccataacga
    19081 cggtgccggt tcgctcgttg accacgatct tggcgacgac atccggaatg acctcgacct
    19141 cttcgatgat ggataagaag gtgatcacat cgtcgaaaaa gacgtcgggc actgtcactt
    19201 tgatggtgga ggcgtccatc gcttttgaga tgcgctctcc gaatttcgtg tcgatcgctt
    19261 gtgagacccg agccgccgtc gtgatgtccg gattcgagag taggaggctg acggatttag
    19321 cgtcgacgat ctgtataggg atttcttttt ccacgatagc gccgcccggg atagtgccaa
    19381 cgaccgtata acgcggttga aggttgatcg acgccttgat gtctgcccca cctaagctga
    19441 tctgaccttg cgagaccgcg tagacgacgc catcggcccc gtacaacgga gtttgcagaa
    19501 gaacgccgtt ggccagggac ttcgcgtctc cgatggaaga gatttgaatg tccaatcgca
    19561 tacccggttt gaagtagggg ggaatatcgg ctgtgacgat cacgatcgcc gcgtttttgg
    19621 actttaagtc ttggttcgag atcgatatgc cgaagttctt cagcatattg ctgatcagcg
    19681 tggaagggac ggttccggag tctccactgc cgttgagccc gatcacgact ccgacgccaa
    19741 acagctggtt gtctcgcgca ccgcggaatc gcgcgatatc cttcaaacgc accaaagcgc
    19801 cgaaggacaa tagagagacg agcatcagga caaccacaca cgcgaaaggt tttttcatcc
    19861 tctgtcaccc gctttaaaag ataattttgg ataagcccga caagatgtag ctgacccagc
    19921 tttgctcgga gggatcctga acgaagacaa catcaccgtt gtaccagatt ttcgcattcg
    19981 cgatcttgaa tgagtcgatc gtgttgctcg cggagacatc ccccggcctc acgaccccct
    20041 ggatgatcag ttcccgccga tcttgcccca ccttcacctc ttttttccct tccaacgtca
    20101 agttcccgaa tccgtctatt tcggtgatga ccgcagcgat cttcgcccgt atctgttgct
    20161 ctgcggtgtt gttcgttttg gcggtggctg cggaaccggt gtcgggcacc gaggatggaa
    20221 ggaatttaaa gaggtcgaca ttgctgatgt tttttataat agataaggtc gacgtgatca
    20281 ggcctttgga cttgttttcg gtcgaatcgc tctgagacag atccaccgtt tccgtgatga
    20341 ggatcgtgac gatatccccc actttgaacg ctttgttgtc gctgtacaaa ttgcgaaact
    20401 tctcattgtt cgaggaggac cagagagaaa acccgaaaca aggggcaatc aacagcaaca
    20461 ttaacgcgac agtgccaatt ttc
//
LOCUS       DAOPXN010000018        20238 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2672_length_20238_cov_5.562404, whole genome shotgun sequence.
ACCESSION   DAOPXN010000018 DAOPXN010000000
VERSION     DAOPXN010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 20238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20238
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_20238_cov_5.562404"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="PKM49_01935"
     CDS             <1..1011
                     /locus_tag="PKM49_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81608.1"
                     /translation="ERVLETVKAGIYNEDQSIVGRYLVNFFVSFLKEDNLFQVKSREE
                     AARLLDEGKIDALLVIPRGFTAMMLVNKPTELIYVPSGASLLESVTIYKLLKMVLAEI
                     QYGAIIEIDLGERMWPSPDVPVPQLKIEGIFNNRLDYPDVMAPGVLAFVILSTMLIGI
                     TTSVSREKDRGLIDAFRVTNANRWSFVFGKFSAYSFLGLIQTLILLFGSVWFFDIHVE
                     GSLLLVGLFLILGMLSYLSLGLLISILSPNGDVSLGIAVGIVFLMFLGGGVFFPISQM
                     PAIMQKIAPFLPITVLTESLRKIMITGRPIDQQWRELAISFGFLFSFLSASLISFKRS
                     TR"
     gene            1037..2905
                     /gene="mnmG"
                     /locus_tag="PKM49_01940"
     CDS             1037..2905
                     /gene="mnmG"
                     /locus_tag="PKM49_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNY81609.1"
                     /translation="MFDFDLIVVGAGHAGCEAALVGARCGKKTLLLSLNLDSVAWTPC
                     NPSIGGPAKGVVVREIDALGGEIAKNTDETMIHIRLLNTSKGYAVRALRAQIDKHRYS
                     ARMAALLHRTRHLTLRYGQVERVIVEKGRATGVETHFGERYSSKAVVITAGTFLNGRI
                     FVGPQELASGRLGELPSERLSESIEALGIRKGRFKTDTPARIARQSINFEAMERQDTS
                     EEPLCFSFFSKPVRLNFENPVFITRTNELTHRIIRENLHLSPYYGDRKLLSGAGPRYC
                     PSIEDKVVKFSDRDSHQVFVEPEGDHCEEYYLGGFSTGLPFDAQIRMVQSLRGLEEAE
                     IVRPAYAIEYDYFFPDQLQPTLETKACEGLYFAGQINGTSGYEEAAGQGFVAGVNAVL
                     KMDGKGPWIPKRTESYIGVMVDDLVTKGVDEPYRLLTSRAEYRLILRNDNAHLRLTKY
                     GHSFGLIDADFYQKVERLRETIETELGRLSSLTLKPSELLNAILLSKGTPPIQNAVKM
                     IDLLRRHQVDYLDLKTFDPDPIPDPEVIEQIRIESQYHSYIERALKEIRKMEELEDFA
                     IPHTLRYGDVPNMATEAKQKMERIKPSSIGHAMRIPGINPTDIANLIFYIRSGHKS"
     gene            2905..4008
                     /locus_tag="PKM49_01945"
     CDS             2905..4008
                     /locus_tag="PKM49_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81610.1"
                     /translation="MRRVLADVLYHPEYLNYLKELRLTSSFVCGELSQSNIRKISAEG
                     LFYVFNRCDQKMAKRLASQELLVLKFGLEELLFTKEPFEKRLREVSELFGLTDWDLAP
                     LLFYTAPSFFFLPREEVRAYVENRFRISTKDSTFYHYNQQLKKAFEGSEEQKSFRKPM
                     EFVAAVMTFFREEERRLELVDKEDSRILEEMADSLLTIDPFRIDPKLVSWLKDLYDSF
                     TETQKGAFRELATRKRLHPYIRRLVTDDERKGAIIDGSNLMMAGLYKPDPRRFLLLLN
                     VMGVHKPLLFPFLIIFDANADHLVQTDRDFWETRFLNNPSVIFHSPADEWILKFAQEK
                     RCAVISNDRFRDYERSSVEILRFAPEKGKLYLT"
     gene            4019..4567
                     /locus_tag="PKM49_01950"
     CDS             4019..4567
                     /locus_tag="PKM49_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY81611.1"
                     /translation="MDFLELCKGRRSTRFFSRAEVSTETLEGILEAGIWAPSGHNEQP
                     WFFSVVRDKSLQDTISVKTKVLMKESPVSWIAKKGADPNFHLFYHASAVIVVSGKTSA
                     YSAIVDCSAATQNMLLMAHSLGLGGCWIGLISPFLKLPEAKELLGIPEDYQGFYAIAL
                     GYPEAGIVPKAPSRKNPQVQWL"
     gene            complement(4635..5867)
                     /locus_tag="PKM49_01955"
     CDS             complement(4635..5867)
                     /locus_tag="PKM49_01955"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNY81612.1"
                     /translation="MTNPFRLREELEHAQLENQTLKDELNRLRAEKKELEADYLSLFS
                     TLQKYERLVQSTTMLIEMAVDPDQDFNKIILDTAFELIPEADYGSISIIKDEDWLFVY
                     AAGHNLEALKSIPFKKSYFLDPRDVDSTLFFDSNVFVVPEAWSIQNMPEEVYRRYREA
                     VLPVRESAVVQIDLANQIAGHLSLDIGTHEEKHFSKESLKVMEALGSITSAFFAFQRL
                     SKLQEEFQREIILSIIRIVEIHDTYTKGHSEHVASISLDLAKEMGLSAEVCRQVYWSG
                     LVHDIGKILVSQSVLNKPDRLSDSEFEMIKNHPVWGYEVLTHSKELREIAIFVRHHHE
                     RYDGNGYPDRLAGEGIPMISRILTVADSWDAMIRDRAYRKGLSREVAIDEIKRNVGLQ
                     FDPNVAKAFMRLCAKGAL"
     gene            complement(5877..6395)
                     /locus_tag="PKM49_01960"
     CDS             complement(5877..6395)
                     /locus_tag="PKM49_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HNY81613.1"
                     /translation="MRKIIIAAVSVNGFLSHSETEGANWTSKADKRFFKEATCGGTGV
                     VIVGRKTYETIPNGLPGRRMIVLTRHPERFQDGPEVEFRSEGAQTLLPELEANGVKEC
                     WIAGGRQVYTYFLREGLVDEIFLTIEPILFGNGIPFLSELAEDRPLSLVSHRLLNDQT
                     VLLHYRTHRTGG"
     gene            complement(6407..7597)
                     /locus_tag="PKM49_01965"
     CDS             complement(6407..7597)
                     /locus_tag="PKM49_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY81614.1"
                     /translation="MKDILTVLKKELRNVLKDRRTLFMLFGVPVIMFILIFNVMGEVM
                     KTQEKKLEETVYKVYTNDVSFVESAFLNGPTKIECVEGTPDAIENDLRDKRYDLAVLV
                     KGQIERGSDLFLDDMEINVYSFSGSMASERIASVFSFAVNTLRDQQLRLLLSDAQLDA
                     GLLDKPRINTINVATEQEQTGAVFGGLLPYMLVIYLFTASFSVGFDTTAGEKERQTLT
                     ILLANRVARSSIAWGKILYLMLMNVLSATISVVAFLFGFSSIIPQTGSMFTMFTPGSA
                     ALLFLIVLSLAVLIAAIITVIGIFARSVKEATSYALPIYVVTILFGILGMQPEVFDSQ
                     GFIRFIPLVNGVFTMKDLVTQPRIDFVTVAITLVINLVAAALLAFLATKMFNDERYVF
                     RTKN"
     gene            complement(7560..8333)
                     /locus_tag="PKM49_01970"
     CDS             complement(7560..8333)
                     /locus_tag="PKM49_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81615.1"
                     /translation="MILFNEVSKVFGTGKKEVRALDNVSFAVQSGEIFGLLGPNGAGK
                     TTSLRLLSTLLKPTNGRISVAGLDTVKESPKVRERIGFLSSDMNLSGSLSAQELFHFF
                     GALNHIEEREIVNRSNRLIDQLKMEDFFQRKVETYSTGMKQKTLIGIALLHDPDIIIF
                     DEPTNGLDIITGRVVLDILKSLKQRGKTLIISTHIMSIAEKLCDRVGIIHKGEIRVIG
                     TLQEIYTQTGQPDLEEAFFSVIGGVIEDEGHPHRSKKRA"
     gene            8867..9643
                     /gene="rlmB"
                     /locus_tag="PKM49_01975"
     CDS             8867..9643
                     /gene="rlmB"
                     /locus_tag="PKM49_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNY81616.1"
                     /translation="MVRVYLYGKNALTELLKSQQNAILKKVYLSPSASLSEREESLLK
                     GLNVSVTVMPLKTIEQLVGKGAAHQGVVLETKDFQYLEYEELLEQLEPAPVASLVFLD
                     QVQDPHNLGAIIRTAYSAGVDGVVTCQDNSASVTPAVVKVSAGSAFLLPICIVNNLRR
                     ALERAKEKGFWIYGTEKDGHPYPLVAFPPKIGLVFGNESSGMRRLVKESCDETVSIPM
                     ANPFDSLNVSVSAGILCFEILRRRLFPETREPQVLKEGEP"
     gene            9645..11399
                     /gene="mutL"
                     /locus_tag="PKM49_01980"
     CDS             9645..11399
                     /gene="mutL"
                     /locus_tag="PKM49_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNY81617.1"
                     /translation="MTIRSLPPEVYMKIAAGEVVANPACVLKELVENALDAQATALTI
                     RFQNGGIDAICVKDNGIGMSETDITQSILPHATSKLSSWEDLSALGTFGFRGEALAAI
                     TAVSETILVSRYIGEETGIRLYVRGGSVLESKRVNVGVGTEIEVRNLFFNVPARRKFL
                     KSPVSEERKLMELLENFLISFPQIRFRIEKDAETLYEVPKQTLLDRLPTLFQGIDRTE
                     WQFLEEHFQGLTASAYIADHKYYRRNRSAIHTFVNGRLVKNPIIYAAIDGAMEPVFAK
                     KSHPFLLLFLTLPTDFVDINVHPQKMEVQFARSEWVFHLVQKLLQKRAQETVPEIALP
                     EAGGAVPQRAEPLERERPAFFRTPSPPVDTAERIYAKPEPFFSKPEPTLMRAARANEF
                     TLEPTQPGSQRVLTVVGRRYVLGEDPDGVFFIDFHAAHERLLFDEIAANNGKVASQEV
                     LEPIPIHLTPLQTNLIEEHRLALEQFGFRFKKQDQRYLLEGIPQKIGLQDGESILLDI
                     LEELRLSTIEPMPEVFRRIFASVACKQAFRTGDTFTVAQAEDLVSLIERKGITTCPHG
                     RPVRFRLTFKELDRFFER"
     gene            11402..12181
                     /locus_tag="PKM49_01985"
     CDS             11402..12181
                     /locus_tag="PKM49_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY81618.1"
                     /translation="MRKTIDEKISHTPPLFIQRLWMGFLFSGYVVYSFFVFSKAERIK
                     KTKGKREWQQYVRPKVRRFCQRCFVWLGMKPQIRHMERLPDTGAYLLIANHQSFFDVS
                     LVLGFLSPNAFLMAKKEFSKLPFFGRAVSQVGYFIDRKDPKQAIASLRKAIEHLKEGT
                     PFALFPEGTRSVDGKMGPFQKNSLKLAYIAKVPIIPVVIEGTQYVMPKGSFFALRAQV
                     KAEVLEAVHPEDHPDEEQLSRFLHSEMQAKKKEMENEKSNH"
     gene            12162..12752
                     /locus_tag="PKM49_01990"
     CDS             12162..12752
                     /locus_tag="PKM49_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNY81619.1"
                     /translation="MKRAIISGLFKDAEHNAPIVQLLIENTNQAVHIWIGGCEAWTLG
                     MTLEKVKLERPLTHDLLISVMRAFQAKPTRVTIHSLKHGVFHAKLFITKFGLPQDEDL
                     EPTNLEVDCRASDGIIIAAKLNLPIYISSEVILETAIPIEVQKKITDEDQKFHEFLQS
                     FNLEELKHHLEKEKADREEGGSFPDTFLEDDDPERE"
     gene            12781..13518
                     /locus_tag="PKM49_01995"
     CDS             12781..13518
                     /locus_tag="PKM49_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81620.1"
                     /translation="MLDELLKQSRASFRKTPRMESDLDLYSVYLDRSSVGEPAKRFEV
                     NLYGGINGAWRVGSTIALLPLYKADGFSRKATKGHWRTFFSELATQYRLHLFLPLGDS
                     CPKGVWYYSDGSTEIIETPIKDSVWYRACRPESPTSRVAFCSPEALIDPSCLSRLAGP
                     EGRLVVVYTPEIASDLEIFAGGRSLQFDLFFLLISPERVYVTGPSIWGADKPCYNKGF
                     HLQKEAELFSCHIPLGFPKYEIDREDT"
     gene            13533..15302
                     /locus_tag="PKM49_02000"
     CDS             13533..15302
                     /locus_tag="PKM49_02000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNY81621.1"
                     /translation="MEMKWSLETLYPSFEDPAFERDMAFVKESLDELTTWVDHHLGKG
                     EHPSETVEGYLNRLLRIYHKAYRISAFANLSLSVEVKNARALKVVERMESFLSTLKVP
                     QVKFRRWLTGLEDFEGLFGSGGLTDEHRFVLTEWRDNGRYLLSDAEEALAAKMAQTGS
                     AAWAKLQNVVSSTLLVEVPLVEGVKKLPLPVVRNMAYDRDQKTRKAAFEAEIESYRSI
                     EDASAACLNGIKGEAITLAKARGYTSVLEKTLRDSRVEKTTLDSMLGAIRDSLPAFGR
                     YFKKKAQLLGHPTGLPFYDLFAPIGDIDLRYTYEEAAAFIIDRFASFSAHLSNFAKRA
                     FENRWIDALPREGKRGGAFCYNVRVINESRIMANFNGSFNNMTTLAHELGHGYHGECL
                     REQSLLNSHYTMPLAETASIFCETIVTDAAYAQASEAAKLAILEHQISSAAQVIVDIY
                     SRYLFETELIQRREDGSLSVAQLKEAMKNAQMKAYGDGLAPEVLHPYMWINKPHYYSA
                     DNNFYNFPYAFGLLFGKGLYAEYLDNREAFVASYPQLLAETGKNSVAEIGSIAGIDVN
                     QTAFWKKSLRMIEEKIDEFIRLA"
     gene            complement(16059..17960)
                     /locus_tag="PKM49_02005"
     CDS             complement(16059..17960)
                     /locus_tag="PKM49_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019914917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81622.1"
                     /translation="MSSGTQSGKGRSLPQNFSLGARPGAGGRGGARFGPPVQKAKDPK
                     GTLKKLVRYLRPHLFGLFVIILLTIGGSVFSVFSPKVLGKAITVLYDGFAAKLAHVPN
                     ASLDFQTIGQTTLVLAVLYLFSILFNYLQQFWMIGITQKIIRQLRSEVMEKLTRLPLK
                     YFDTHTHGEILSRVTNDVDNLSNTMQQSLTQLLSSLVTLCGVTIIMFTINAPLAALTL
                     VTFPLSLFVISFVTKRSQRYFIQQQKSLGQLNGHVEEMYGGHIAVKSYNQEKTSIAGF
                     SHYNDELFQSGWKANFISGLIMPMMHIIGNLGYILVAIVGGIFVARRTITLGDVQAFF
                     QYSKQFNQPVTQVASIMNLIQSTIASAERVFEILEEAPEPADPETRGFVEPVEGRVAF
                     ENVSFRYLPNKPLIDDLNLSIEPGQMVAIVGPTGAGKTTLVNLLLRFYDIQKGRLLVD
                     ETDIRSFPRSELRNIFGMVLQETWLFNGTIRENIAYGKEGATAEEVIAAAKAANAHHF
                     IRALPEGYDTFINEEASNLSQGEKQLLTIARAFLRDPDILILDEATSSVDTLTEVSIQ
                     RAMRDLTQGRTSFVIAHRLSTIKNADIILTMNEGKIIETGNHRQLLEKRGFYADLYAS
                     QFTGRQAVS"
     gene            complement(17957..19687)
                     /locus_tag="PKM49_02010"
     CDS             complement(17957..19687)
                     /locus_tag="PKM49_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81623.1"
                     /translation="MKNVFLYFKPYRWWALLSISLVVTRAMTELYLPNLMSKIVDRGI
                     ATGNIELILRTGLAMLGVTAITFVVIFCTSFLSSQISARFARDLRKGIFEKVETFSLE
                     DVESFGIASLITRTTNDVSQIQQALMMSLRMVVMAPVMAVGSVIMAFWKNARLSSILF
                     VSIPFLLFGVYFITRKAIPFFRAMQDKVDRLNQVLRENLTGIRVIRAFGKSDYEKRRF
                     VETNEDLTTTSLRVHRIVALVTPVIMLVMNVTILALLVFGSFQIDTGTLQIGELMAII
                     QYVLHITNAFIMMSMVFSFLPRAEVSIRRIEEVLSRPASIENSEEAVPLPYQPGATVT
                     FEEVTFRYPGASEPALNHISFTAESGKTTAIIGSTASGKTTLLNLLMRFYEVDQGSIM
                     INGVDIKKAKLEDIRGKIGYATQKAVVFTGSVKENIRFGREEITDAQVERAMRIAQLE
                     PFVNAMPQKADTVIAQGGTDLSGGQRQRLSIARAIAADPPILLFDDTFSALDFKTDAK
                     LRLALRAEMRNKTLLIIAQRVSTIMDADQILVLKNGVLAGKGSHAQLMRDCPEYREIV
                     YSQLSEEEIA"
     gene            complement(19702..20148)
                     /locus_tag="PKM49_02015"
     CDS             complement(19702..20148)
                     /locus_tag="PKM49_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNY81624.1"
                     /translation="MESAFDDEIGELLFQIHRLYHIRMSALVKALEISPRQIPILKHL
                     YRSGVANQRQLAEKACVSPTAITLMLRKMEAKGLVEKKTDPADRRFFSVSLTEKGKSV
                     VEQLKRLRKSIDGITVIGFTQEEQAHLQSLLRTVRQNLLEQISANA"
BASE COUNT         4869 a         5350 c         5421 g         4598 t
ORIGIN      
        1 gaaagagtac tggagacggt caaggcgggg atctataacg aagaccagtc gatcgtaggc
       61 cgatatctcg tcaatttttt cgtctctttc ttgaaagagg acaacctttt tcaggtaaaa
      121 agccgagaag aagccgcacg gctgttggat gagggaaaga tcgatgcgct cctggtgatc
      181 ccccgaggat tcaccgcgat gatgctcgtc aacaaaccaa ccgagctgat ctatgtaccg
      241 agcggtgcct ctcttttgga aagtgtgacg atttacaaac tcctcaagat ggtcttggcg
      301 gaaatccagt acggcgcgat tatcgagata gacctcggag agcgaatgtg gccttctccg
      361 gatgtgcccg tcccccagtt aaaaatcgag gggatcttca ataatcgctt ggactatccg
      421 gatgtgatgg cgcccggggt gttggcattc gtcatcctat cgacgatgct gattggaatc
      481 acgacctcgg tcagtcgaga aaaagacaga gggttgatcg acgcctttcg agtcacgaac
      541 gccaatcgct ggagcttcgt gtttggaaaa ttcagcgcct actccttcct tgggcttatt
      601 caaacgttga tcttgctgtt cggtagcgtg tggttcttcg atatccacgt cgaagggtcg
      661 ctcctgctcg tcggtctctt cctgatcctg ggaatgctga gttacctttc tttagggcta
      721 ctgatctcga tcttgtcacc gaacggcgac gtctcgttgg ggatcgcggt ggggatcgtc
      781 ttcttgatgt tcttgggtgg tggcgtcttc ttccctattt cgcagatgcc ggcgataatg
      841 cagaagatcg cgcccttctt gccgatcacg gtactgaccg aatccctacg aaagatcatg
      901 atcacgggac ggcctatcga tcaacagtgg cgggagctgg ctatttcatt cgggttttta
      961 ttctcgttct tatccgcttc tttgatctct ttcaaaaggt cgacgcgata gtggagctca
     1021 aaggggtgag cgagacatgt tcgattttga cctaatcgtc gtcggtgccg gccacgcggg
     1081 atgcgaagcg gcgttggtgg gagcccggtg tggaaagaag accttgttgc tcagtctcaa
     1141 cctcgattct gtcgcctgga ctccgtgcaa tccctctata ggcgggcctg ccaaaggggt
     1201 cgtcgtgcgc gagatcgacg cgttgggcgg agaaatcgcg aaaaatacgg atgaaacgat
     1261 gattcatatc cgcctgctca atacctccaa aggctacgcg gtccgagcgc ttcgggctca
     1321 gatagacaaa caccgataca gcgccagaat ggccgcgctc ttgcaccgca cgcgacactt
     1381 gacgttgcgg tacggccaag tcgaacgcgt gatcgtggaa aaaggccggg caacgggtgt
     1441 ggagacccac tttggagaac ggtatagctc gaaagcggtg gtcatcaccg ccggaacctt
     1501 cctaaacggg aggatcttcg tcggcccgca ggaactggct tctggccggt tgggagagct
     1561 cccgtcggag cggctctcgg agtctataga ggcgttggga atccgcaaag ggcggttcaa
     1621 aacggacacc cccgcgcgga tcgcccgaca atccatcaac ttcgaggcga tggagcgcca
     1681 ggacaccagt gaggaaccgc tctgtttttc gtttttttcc aaaccggtcc gactcaattt
     1741 cgaaaacccg gtcttcatca cccgaacgaa tgagctcacc caccggatca tccgcgaaaa
     1801 ccttcaccta tccccgtatt atggagatcg caagctgctc tccggagcgg gaccccgata
     1861 ctgcccgtca atcgaggaca aggttgtcaa gttttccgat cgagattccc atcaggtctt
     1921 cgtcgaaccc gaaggcgacc actgtgagga gtactatttg ggcggttttt ccacgggcct
     1981 gccctttgac gcccagattc ggatggtcca aagcttacgc gggttggaag aggcggagat
     2041 cgtccgtccc gcctacgcga tagagtacga ttactttttt cccgatcagc tccaacccac
     2101 actagaaacg aaggcctgcg aagggctgta ctttgccggg cagatcaacg ggacgagcgg
     2161 gtacgaggaa gctgctgggc aaggattcgt cgcgggcgtt aacgcagtgc tcaagatgga
     2221 cggaaaaggc ccttggattc ccaagagaac cgaatcctat atcggcgtga tggtcgacga
     2281 tctggtcacc aaaggcgtag acgaacccta ccggctgctc acctcacgcg cggagtaccg
     2341 actgatccta agaaacgaca acgcgcacct gcggctcacc aaatacgggc actcctttgg
     2401 actgatcgac gcagacttct atcaaaaggt ggaaaggcta cgcgagacca tcgaaacgga
     2461 attggggcga ttatcctcct tgaccctcaa gccttccgaa ctgttgaacg cgattttact
     2521 gagcaaggga accccgccta tacagaacgc ggtgaaaatg atcgatctat tgcgcaggca
     2581 tcaagtcgac tacctcgatc taaaaacctt cgatccggat ccgatccccg acccagaagt
     2641 gatcgaacaa atccgtatcg aatcccaata ccacagctac atagaacggg ctctcaagga
     2701 gattcgcaag atggaagagt tggaggattt cgcgattccc cacaccttgc ggtatggaga
     2761 cgtgcccaat atggcgacgg aggccaaaca gaagatggag cgcatcaaac cctcctctat
     2821 cggacacgcg atgcgcatac ccggcatcaa cccaacggat atcgcgaacc tgatctttta
     2881 catacgcagt gggcataagt cctgatgcgc cgcgttctcg ccgatgtcct ttaccatccc
     2941 gaatacctga attacttgaa ggagttacga ctcacttctt ccttcgtttg tggagaattg
     3001 tctcagtcaa atatcagaaa gatatcggcg gaagggcttt tctacgtctt caaccgttgc
     3061 gaccagaaaa tggccaagcg actcgcctca caggagttgc tggtattgaa attcggtttg
     3121 gaagagctgc tgttcactaa ggagcctttt gaaaaacggc tgagggaagt ttcggaactc
     3181 ttcgggctta cggactggga cctcgcgccc ctgttgttct ataccgcgcc ttcgtttttc
     3241 tttctaccgc gagaggaggt gcgcgcatac gtagaaaacc gctttcggat atcgacgaaa
     3301 gattctactt tttatcacta caatcagcag ctgaaaaagg ccttcgaagg atccgaagaa
     3361 cagaagtctt tccgtaaacc gatggagttc gtggcggcag tgatgacctt tttccgtgag
     3421 gaagagcggc gtctcgagtt ggtggataaa gaggacagcc gaatcctgga ggagatggcg
     3481 gattcgcttt tgacaatcga tccctttcga atagacccga aactagtctc ttggttaaaa
     3541 gacctctatg attctttcac tgaaacgcag aagggcgcat ttcgggagct ggcgacaaga
     3601 aagcgactgc acccctatat ccgtcgattg gtgacagatg atgagcgtaa aggcgcgatc
     3661 atagatggga gcaacctgat gatggccggg ctctacaagc ccgacccgcg acggtttttg
     3721 ctccttctga acgtgatggg cgttcataaa ccgctactgt tccctttttt aatcattttc
     3781 gacgctaacg ccgaccatct ggtgcaaaca gatcgcgact tctgggaaac gcgatttctc
     3841 aacaatccgt cggtcatttt tcactcccct gcggatgagt ggattttgaa gttcgcgcaa
     3901 gaaaaacgct gcgcggtgat ttcgaacgac cggtttcgag attacgagcg ctcttcggtt
     3961 gagatcctac gatttgcgcc ggagaaagga aaactgtatt taacatagag gagggcatat
     4021 ggatttctta gaactgtgta aaggacgcag aagcactcga tttttctcgc gcgcggaggt
     4081 ttcaaccgag acactggaag ggattttgga agcgggaatt tgggctccat ccgggcataa
     4141 cgaacagccg tggttctttt ctgtcgtccg cgacaagtca ttacaggata ccatcagcgt
     4201 gaaaacgaag gtgttgatga aagagagccc cgtttcctgg atcgcgaaaa aaggggcgga
     4261 cccgaatttt catcttttct accacgcctc agctgtcatt gtggtttcgg ggaaaacctc
     4321 agcctattcg gccatcgtcg attgtagcgc ggccacgcaa aatatgctgt tgatggcgca
     4381 ttcgctgggg ttgggggggt gctggatcgg gctgatcagt ccctttctca agctgccgga
     4441 agcgaaggag ctcttgggta tccccgagga ctatcaaggg ttttacgcga tcgctttggg
     4501 ctaccccgaa gcgggaatcg ttccgaaagc gccgtcgcga aagaaccctc aggttcaatg
     4561 gctgtaaaaa aacggatcgc ctgaccgtcc aaactgggaa acgccccttc actggtcgcg
     4621 gtgggatgcg ctctctataa ggcgcctttg gcgcacagcc gcataaaggc cttagcgacg
     4681 ttgggatcga actggaggcc gacgtttctc ttgatctcat cgatcgcgac ctctcgggag
     4741 agtccctttc gataggctcg gtctcggatc atcgcgtccc aagaatctgc gacggtcaga
     4801 atccgagaga tcatcgggat gccttctccg gcaaggcggt cggggtaccc gttgccatcg
     4861 tatcgctcgt ggtgatgacg cacgaaaatc gctatttcgc gcaactcctt cgagtgggtg
     4921 aggacttcat acccccacac cggatggttt ttaatcattt cgaactcgct atcggaaagc
     4981 cggtctggtt tgttcagaac ggattgggag acgagaatct ttccgatatc gtgtacgagt
     5041 ccagaccagt agacctgtct gcacacctcg gccgagagcc ccatctcctt cgccaagtcc
     5101 aaagagatcg acgccacgtg ttcggaatgg cccttggtgt aggtatcgtg tatttccacg
     5161 atacgtatga tcgagaggat gatctccctc tggaattctt cctggagttt ggatagcctt
     5221 tgaaaggcga aaaacgcgga ggtgatgctg cctagggctt ccatcacttt cagcgactct
     5281 tttgaaaaat gcttctcttc gtgcgtcccg atgtccaacg acaggtggcc ggcgatttgg
     5341 tttgccagat cgatctgcac gaccgccgac tcgcgcacgg gcagaaccgc ttcccgatat
     5401 ctgcggtaga cctcttccgg catattttgt atactccagg cttcaggcac gacgaagacg
     5461 ttggagtcga aaaacagcgt cgaatcaacg tctcggggat cgaggaagta agacttcttg
     5521 aacggtatgc tcttcaacgc ctccaagtta tggccagccg cgtagacgaa tagccagtcc
     5581 tcgtccttga tgatggatat gctgccgtaa tcggcctcgg gaatcagttc gaaggcggtg
     5641 tccaaaatga tcttgttgaa gtcttgatcg ggatcgacgg ccatttcgat cagcatcgtc
     5701 gtagactgca ccaaccgttc gtatttttgt agggtggaaa acaaagaaag gtaatcggct
     5761 tcgagttctt tcttttccgc acggagccga ttcagctcat ctttcagcgt ctggttttcc
     5821 agttgtgcgt gttccagttc ctccctcagg cgaaacgggt tcgtcatctt gcccttctat
     5881 cctcccgtac gatgcgttcg atagtgaagc aacaccgtct gatcgttgag taaccgatgg
     5941 gaaaccaagg aaagaggcct atcctcggcc aattccgata aaaacgggat gccatttcca
     6001 aacaggatcg gctcgatggt gagaaatatc tcgtctacga gcccttctct caagaagtag
     6061 gtgtagacct gtctaccccc ggcgatccaa cactctttaa ccccgttggc ctccagctcc
     6121 ggcagaagcg tctgcgctcc ctcagaacga aactcgacct ccggaccgtc ctggaagcgt
     6181 tccggatgcc tcgtgagaac gatcatccgc ctaccgggca acccgttggg gatcgtttcg
     6241 taggttttcc tcccgacgat gacgaccccc gttccaccac aggttgcttc cttaaaaaag
     6301 cgtttgtccg ctttcgaagt ccaattcgcg ccctcagtct cagaatgcga caaaaatccg
     6361 ttgacggaaa cggccgcgat gatgattttc cgcacggagg gtttcctcag ttcttcgttc
     6421 taaagacgta tctttcgtcg ttaaacattt ttgtggcgag aaaggcgagc agcgcggccg
     6481 cgaccaggtt gatcaccaag gtgatcgcca ccgtcacgaa atcgatccgt ggctgcgtga
     6541 cgagatcctt catcgtaaaa acgccattga ccagcgggat aaaccggatg aatccctggc
     6601 tatcgaagac ttcaggttgc atgcccagta taccgaagag gatcgtcacc acgtagatcg
     6661 gcaacgcgta ggaggtggcc tctttcacgg agcgcgcgaa gatgccgata acggtgatga
     6721 tcgcggcgat gaggacggca agtgagagca cgatcagaaa caacaacgcg gcggaacccg
     6781 gggtgaacat cgtgaacatg ctccccgttt gcggtatgat cgaagagaat ccgaatagga
     6841 aagcgaccac cgatatcgtg gcgctgagca cattcattaa catcaggtag agaatctttc
     6901 cccacgcgat agacgaacga gccacacggt tggcgagcaa aatcgtgagc gtttgccgtt
     6961 ctttttcgcc ggctgtcgta tcgaaaccca cggaaaaaga ggcggtgaac aggtaaatga
     7021 ccaacatata gggtagtaag cctccgaaaa ccgcgccggt ttgctcttgt tctgttgcca
     7081 cgttaatcgt atttatccta ggcttgtcca gaagacccgc atccaactgt gcatcggata
     7141 ataacaaacg cagctgctga tctcgaagcg tattgacggc aaaagaaaag acgctcgcga
     7201 ttctttcgga ggccatagaa cccgagaaag agtacacgtt gatctccata tcgtcaagga
     7261 acaagtcaga accgcgttcg atttgtcctt tcaccaagac ggccaaatcg tagcgtttgt
     7321 ctcgcaagtc attttctatg gcgtctggtg ttccctcgac gcattcgatt ttggtcggtc
     7381 cgttcaagaa ggcgctttcc acgaaggaca cgtcattcgt atagactttg taaaccgtct
     7441 cctccagctt cttctcttgg gttttcataa cctcccccat cacattgaag atgagaataa
     7501 acataatgac gggaacgccg aagagcataa acagcgttcg tctatccttg agtacattcc
     7561 taagctcttt ttttagaacg gtgaggatgt ccttcatctt ctatcacacc tcctatcaca
     7621 gaaaaaaagg cttcctcgag gtccggctgt ccggtttgag tatagatctc ctgaagcgtc
     7681 ccgatgacac gaatctcccc cttgtggatg attccgacac gatcgcagag tttctcggcg
     7741 atactcataa tgtgggtgga gatgatcagc gtcttacccc gttgcttcaa gctctttaaa
     7801 atatccaaca ccacccgtcc ggtgatgatg tccaatccgt tggtcggctc gtcgaagatg
     7861 atgatatcgg gatcgtggag caatgcgata ccgatcaacg ttttctgctt catcccggtc
     7921 gagtaggttt cgacctttct ttgaaagaag tcttccatct tcagttgatc gatcaagcgg
     7981 ttcgagcgat tgacaatctc tctctcctcg atatggttca gcgccccgaa gaagtgaaag
     8041 agctcttgag cggacaaact ccccgacaag ttcatatcgg acgaaaggaa accgatacgc
     8101 tctctgacct tcggggattc cttcaccgta tctaaccccg ctaccgaaat ccggccattc
     8161 gtcggtttca gcaaggtaga taagagcctt aacgacgtcg tcttaccggc gccattggga
     8221 cccagaagcc cgaatatctc cccggattga actgcgaagg agacattgtc caaggcccgg
     8281 acttcctttt tgcctgtccc gaagaccttt gaaacctcgt taaacagaat caatgagaac
     8341 cctcctttag cgaaagatac gaacgtctga ttatactcta tttcggttca tttccgatga
     8401 acaaatttct cgtagaacga acgtactcgt ccccggagcg caactctggg agcgccactc
     8461 tttggtgcgg catagttttg ggaacgccaa ctttaaagtt ggcatgtctt atgaagaaaa
     8521 aagggctcac acagagggcg cagagagaac atagatcgcg gcgtaagcga ttcttctttt
     8581 cgcgacggcg cgatacgcat ttgtatatgt ctggaaaaac agggttcgca cggagggcac
     8641 agagggcacg gagagaacaa tgaacccgtg ccacactaaa gtgtggcgtt ctcaggcttt
     8701 cacagggcgg atcgcggggt cccgttggtt accccgcgat aacacacgag aagccagccc
     8761 gctggttacc ccgcgatgaa cctcttagtt atgtcacccc ataacttgac ccacaccacc
     8821 caaacggtgg agcccgcggc cgtggtataa tccaaaaagg agaggagtgg tcagagtgta
     8881 tctatacgga aagaacgcgc taaccgagtt gctgaaaagc caacagaacg cgattctcaa
     8941 gaaagtgtat ctttctcctt ctgcgagctt atccgagcgg gaagaatcgc tcctcaaagg
     9001 tctgaacgtg tcagtcaccg tgatgccttt aaaaacgatc gagcagttgg ttgggaaagg
     9061 ggctgcccat cagggggtgg ttctcgaaac caaagatttc caatatctgg aatacgaaga
     9121 gctcctagaa cagctcgaac ccgcgcccgt cgcctccctc gtcttcttgg accaggtgca
     9181 ggatcctcac aatctgggcg ccatcatccg aacggcttac tccgccgggg tagacggagt
     9241 agtgacctgt caggacaaca gcgcctccgt cacgcctgcc gtcgtgaaag tgtccgccgg
     9301 gagcgctttt ctgttgccca tctgtatcgt caacaacctc cgccgagcct tggaacgggc
     9361 gaaggagaaa gggttctgga tttacggaac cgagaaagac ggacaccctt atccactggt
     9421 ggccttcccg ccgaaaattg gcttggtttt cggaaacgaa agcagtggga tgcgccgctt
     9481 ggtcaaggag agctgtgacg agacggtctc catcccgatg gcgaatccgt ttgattcctt
     9541 gaacgtctcc gttagcgcgg ggatactctg ttttgaaatc ctccggcgcc ggctcttccc
     9601 agaaacgaga gagccgcagg tcttgaagga gggggaaccc taagatgaca atacggagcc
     9661 tgccccctga agtctatatg aagatcgccg cgggcgaggt ggtcgccaat cctgcctgcg
     9721 tgctgaaaga actggtggag aacgcgctcg acgcgcaggc caccgccctg acgatccgtt
     9781 ttcaaaacgg gggcatcgac gccatctgcg tcaaggataa cggcatcggc atgtccgaaa
     9841 cggacatcac gcaatcgatc ctgccgcacg cgacgagcaa actctcgagt tgggaagacc
     9901 tctccgcctt gggaaccttt ggatttcggg gcgaggcgct cgcggcgata acggcggttt
     9961 ccgaaacgat cctcgtttcc aggtacatcg gcgaagagac cggaattcga ttgtacgttc
    10021 gcggcgggag cgtgctggaa tccaagcggg tcaacgtcgg ggtcggaacg gagatcgaag
    10081 tgcgaaacct gttcttcaac gttcccgcgc gcaggaagtt cctgaaatct cccgtttctg
    10141 aagaacgaaa actgatggag ctgctagaaa acttcttgat ctcctttccg caaatccggt
    10201 tcagaataga aaaagatgcc gagacgttgt acgaagtgcc caaacagacg ctgttggatc
    10261 gcctaccaac gctttttcaa ggtatcgata gaacggaatg gcagtttttg gaagaacact
    10321 tccaagggct aaccgcctcg gcttacatcg ccgaccacaa gtattatcga cggaatcggt
    10381 cggccattca caccttcgtg aacgggcgcc tcgtgaaaaa cccgattatc tatgccgcca
    10441 tcgatggcgc gatggagccg gtattcgcaa aaaagtcaca ccctttcctt ttactgttct
    10501 taactctacc gacggacttc gtcgatatca acgttcatcc ccaaaaaatg gaggtgcagt
    10561 tcgcccgttc cgagtgggtt tttcacctgg ttcagaaact gctgcagaaa cgggcgcaag
    10621 aaacagtccc ggagatcgcg ctccccgaag ccggcggagc ggtccctcaa cgggcagagc
    10681 cactcgaacg agaacgcccg gcgtttttca ggaccccttc tccaccggtg gacacggcag
    10741 agaggatcta cgcgaagccg gaaccctttt tttcgaagcc agagcccacg ttgatgaggg
    10801 ctgcgcgagc gaacgagttt actctagaac cgacgcaacc cgggtcacaa cgggtattga
    10861 cggtcgtcgg tagaagatac gtactcggcg aagacccgga cggggtgttc ttcatcgatt
    10921 tccacgccgc ccacgagcgg ctcctttttg acgagatcgc cgctaataac gggaaggtgg
    10981 catctcaaga ggtgctggag ccgatcccaa tccacctgac tcccttgcaa acgaatctga
    11041 tagaagagca ccgccttgcc ttggaacagt tcggtttccg gtttaagaaa caggaccaac
    11101 ggtatctgtt ggaaggcatt ccccaaaaga tcggattgca agatggagaa tcgatcctct
    11161 tggatattct agaggagctg cgcctgtcca ccatcgaacc gatgcccgag gtattccggc
    11221 gcatttttgc ctccgtcgcg tgcaagcaag cctttcgcac aggagacact ttcacggtgg
    11281 cacaagcgga ggatttggtt tctctaatcg agcggaaggg tatcacgacg tgcccgcacg
    11341 gcaggcctgt acgcttccgc ctcacattta aagaactcga tcgcttcttc gagaggtaac
    11401 gatgagaaaa acgattgacg aaaagatatc gcacaccccg ccccttttca ttcagcggct
    11461 ttggatgggg ttcttgttct ccgggtacgt cgtgtacagc tttttcgtgt tctcgaaagc
    11521 cgaacggatc aagaaaacga aggggaagcg agagtggcaa cagtacgttc gccctaaagt
    11581 gcgacgattt tgtcagagat gctttgtttg gttagggatg aaaccccaga tacgtcatat
    11641 ggaacggttg cccgacacag gggcctacct actcatagcc aatcatcaaa gcttcttcga
    11701 tgtcagcctc gtgttggggt tcctgagtcc gaatgcgttc ttgatggcga agaaggagtt
    11761 cagtaaattg cccttcttcg gaagagcggt gagtcaagtc gggtacttta tcgaccggaa
    11821 agacccgaaa caagcgatcg cctctctgag aaaagcgatc gaacatttaa aagagggcac
    11881 gcctttcgct ttgtttccgg aaggtacgcg gagcgttgac gggaagatgg ggccctttca
    11941 aaagaacagc cttaagttag cctatatcgc gaaggttccg atcattcccg tcgtcatcga
    12001 aggcacgcaa tacgtgatgc cgaaagggtc ttttttcgcc cttcgggcgc aggtcaaggc
    12061 cgaggttttg gaagctgtgc atcccgaaga ccatccggat gaggaacaac tcagtcgctt
    12121 tcttcattcg gagatgcaag cgaagaaaaa ggagatggaa aatgaaaaga gcaatcatta
    12181 gcggtttgtt caaggatgcg gaacacaacg cgccgattgt ccaactgctt atcgaaaaca
    12241 cgaatcaagc cgttcatata tggataggcg gatgcgaggc ttggacattg ggtatgacgt
    12301 tagaaaaagt gaagttggaa aggcccctca cacacgatct gctgatcagc gtcatgcgcg
    12361 cattccaggc caaacccacg cgggtgacga ttcactccct aaaacacggc gttttccacg
    12421 ccaaactctt catcaccaaa tttggactcc ctcaagacga agacttggaa ccgacgaacc
    12481 tggaggtcga ttgccgcgcg tccgacggga tcattatcgc cgcgaaactg aacctgccga
    12541 tctatatttc cagcgaggtg atcttggaaa ccgcgatacc catagaggtt caaaagaaaa
    12601 taaccgacga agaccagaag ttccacgaat tcctgcagtc cttcaatctg gaagaactga
    12661 aacaccattt ggagaaggag aaggccgatc gagaagaggg agggtcgttt ccggacacct
    12721 ttttagaaga cgatgatccc gagcgtgagt gaatggtggc gggagcggaa ggcgaaacac
    12781 atgctggacg aactcctcaa acaatcacgg gcatcttttc gcaaaacccc gagaatggaa
    12841 tccgatctcg acctgtactc ggtctacctc gacagatcct ccgtggggga gccggccaaa
    12901 cgatttgagg tgaaccttta cggcggcatc aatggcgcgt ggcgcgtagg gtccactatc
    12961 gcgctcttac ctctatataa agccgacggt ttctcgcgaa aagccacgaa aggccactgg
    13021 cgcacgtttt tttcggaact ggcgacacag taccgtctac acctctttct tcccctcggc
    13081 gactcttgcc cgaagggagt ctggtattat tcggacggtt ctacggaaat aatcgagacc
    13141 ccaatcaaag acagcgtatg gtatagggcc tgccgaccgg aaagcccaac ctctagggtg
    13201 gctttttgca gcccggaagc tcttatagac ccatcgtgtc tgtcccgtct cgccgggcca
    13261 gagggccgat tggtggtggt ctatactccg gaaatcgcga gtgacctcga aatcttcgca
    13321 gggggccgga gcctacaatt cgaccttttc ttcttgctga tttcgcccga gcgtgtctat
    13381 gtcacagggc cgtcgatttg gggagccgac aagccctgtt ataacaaggg ctttcatcta
    13441 caaaaggaag ccgagctgtt ttcttgccac atccccttgg gtttccctaa atatgaaatc
    13501 gatagagaag acacctaatg aggaggcagc atatggaaat gaaatggagt ttagagacgt
    13561 tatatccatc ctttgaagat ccagctttcg agagggatat ggccttcgtc aaagagtcgt
    13621 tggatgagtt gacgacctgg gtagaccacc acctcggcaa aggcgaacac cctagcgaaa
    13681 cagtggaggg gtatctgaac aggcttttga ggatttacca caaagcctat cgaatctcgg
    13741 ctttcgcgaa ccttagccta agcgtcgaag tgaaaaacgc gagggctttg aaagtggtgg
    13801 agcgtatgga gtcctttctt tccacactga aggtacccca ggtgaaattc cgacgttggc
    13861 tcaccggact cgaagacttt gaaggactgt tcggaagcgg cggactgacg gacgagcatc
    13921 ggttcgtcct caccgaatgg cgtgacaacg gccgttatct cctgagcgac gcggaggaag
    13981 ccttagcggc gaagatggca cagacgggat ccgccgcgtg ggccaagctc cagaacgtgg
    14041 tctcttccac cttgctcgtg gaagtgcctt tagtagaggg ggtgaaaaag ctgccgttac
    14101 cggtagtcag gaatatggct tacgatcgag accaaaaaac tcgaaaggcc gctttcgaag
    14161 cggaaataga aagctaccga tcgatagagg acgcttcggc cgcctgtttg aacgggatca
    14221 agggggaagc gatcacgctg gcgaaagcca gaggatacac atcggttctg gagaaaacgc
    14281 ttcgggattc gcgcgtagag aaaaccacgc tggacagcat gctgggggcc atcagggact
    14341 ctttgcccgc tttcggaaga tacttcaaga aaaaagcgca gctactcggt cacccaacag
    14401 gattgccttt ctatgacctg ttcgccccga tcggggacat agacttgcgg tacacctacg
    14461 aagaagctgc tgcctttatt atcgatcgct tcgcctcttt cagcgcccac ctgtcgaact
    14521 tcgcgaagcg cgccttcgaa aaccggtgga tagacgcgct cccgagggag ggaaagcggg
    14581 gcggggcgtt ctgctacaac gtgagagtga tcaacgagag ccgcattatg gcgaatttca
    14641 acgggagttt taacaatatg accaccctcg cccacgaact ggggcacggg tatcacggag
    14701 agtgcttgcg ggaacaatcg cttctcaaca gccactacac gatgccgttg gccgaaacgg
    14761 cctcgatctt ttgcgaaacg atcgtcaccg acgccgccta cgctcaggcg agcgaggcgg
    14821 cgaaactggc catactggag caccagatca gctcggccgc ccaagtcatc gtcgatatct
    14881 acagtcgcta tctctttgaa acggagctga tccagcgccg tgaggacggt tcattgagtg
    14941 tggcgcagtt gaaagaagcg atgaaaaacg cccaaatgaa ggcttacggc gacgggttgg
    15001 ccccggaggt cctgcacccc tatatgtgga tcaacaagcc ccattactat agcgccgaca
    15061 acaactttta caacttcccc tacgctttcg ggctcctgtt cgggaaggga ttgtacgccg
    15121 agtacttaga taatcgcgag gccttcgtcg cttcctaccc tcaactgctc gcggaaaccg
    15181 gtaaaaacag cgtcgccgaa attggaagta tcgctgggat cgatgtcaac cagacggcgt
    15241 tttggaaaaa atctctgcga atgatcgaag aaaagatcga cgagttcatt cgcttggcct
    15301 gaagggctga atattcgact ccgtgtggag ccgatttcga gtcggcctgt atctctggga
    15361 acacctgaaa atgccttggg aacgccaact tttaagttgg catgtttcaa aaacgtaaga
    15421 cgggagagag cgcatcgctg gaattcgtta gtatcgcgga atctcgacgg tcatcgcggc
    15481 gtaagcgcac cttcttttcg cgacggcgcg ataccccggt tttcgtttgt tcaaaagata
    15541 aaaaacaggg ttcgcacgga ggacacggtg tagagattat aaccaaccca gaatttagtg
    15601 tggaacgcta gcctgagaac gccgaactat agtgctgcat gttttttgaa aagagaaagg
    15661 caacagagcg tatcgcgggg tcccgttggt taccccgcga taacccgtga catgccaaac
    15721 tgaagttcgg cgttcccagg gcacaccaca aaagtaaaag gctaatcgcg agacttcgcg
    15781 ggcttccgaa gtcagcccgc tggttacccc gcgataaccc cacaaggtca tcgcggcgta
    15841 aacgcttctt ctttccgcga cggcgcgata cgcttctgta cgagttgatc gcggcgtaga
    15901 aggggctaag ggcgtatcgc ggggtcgtaa aaaccactta ccccgcgata accccacaca
    15961 ccgaaatcag cccgccggtt accccgcgat gaacctctta gttatgtcac cccataactt
    16021 gacccacacg atttggccgc ttactctata actatgcctc aagagaccgc ctgacgcccc
    16081 gtgaactgac tcgcgtacag atcggcgtaa aacccgcgtt tctcgagaag ctgtcgatga
    16141 ttcccggttt ctatgatctt gccctcattc atcgttaaga tgatatcggc gtttttaatg
    16201 gtggagagtc gatgcgcgat cacgaagctg gttctaccct gtgtcaggtc cctcatcgcc
    16261 cgctgtatgg agacctcggt caacgtgtcg acgctgctcg tcgcttcgtc cagaatgagg
    16321 atgtcggggt ctcttagaaa cgcccgggcg atggtcaaga gctgtttttc gccttgggag
    16381 aggtttgagg cctcttcgtt gatgaaggta tcgtaccctt ctggtagagc ccgtatgaag
    16441 tggtgggcgt tcgccgcttt cgccgcggcg atgacctcct cagcggtcgc gccctcttta
    16501 ccgtaggcga tgttctcgcg gatcgtcccg ttgaacaacc aggtctcttg aagcaccatc
    16561 ccgaatatgt tccgcaactc gctccgtggg aagcttcgaa tgtccgtttc atccaccagt
    16621 aaacggcctt tctgtatatc gtaaaaacgc aacaagaggt tcacgagggt ggtcttcccc
    16681 gcgccggtcg ggccgacgat ggctaccatc tgacctggct cgatcgacag gttcaggtcg
    16741 tcgatgagcg gtttgttcgg caaataacgg aaggagacgt tttcgaatgc gactcgccct
    16801 tccactggct cgacgaatcc tcgcgtctcg gggtcggccg gttccggagc ctcctccaga
    16861 atttcaaaaa cccgctcggc cgaagcgatc gtcgattgaa tcaaattcat aatgctcgcc
    16921 acttgggtca ccggttggtt gaactgcttc gaatactgga agaaggcttg aacgtccccg
    16981 agcgtgatcg tccgtctggc gacgaaaatt ccgccgacaa tcgcgactaa gatgtagccc
    17041 agattcccaa tgatgtgcat cattggcata atcaaaccgg agatgaagtt cgccttccat
    17101 cccgattgga agagctcgtc attgtagtga gagaacccag cgatcgaggt tttctcctga
    17161 ttgtaggatt tgactgcgat atggccgcca tacatttctt cgacatgccc gttcaattgg
    17221 cccaaggact tctgctgctg gataaaatac cgctgggagc gtttggtgac gaaggagatc
    17281 acgaaaaggc tcaatgggaa agtcaccaag gtcagagcgg ccaatggcgc attgatggtg
    17341 aacatgatga tcgtaacgcc gcacaacgtg accaaagaag agagcaattg agtcaaactc
    17401 tgctgcatgg tgttgctgag gttgtccacg tcgttggtca cccggctgag tatctctccg
    17461 tgagtgtggg tatcaaagta cttgagcggg agtcgcgtca acttttccat aacttctgaa
    17521 cgaagctgcc gaatgatctt ttgcgtgatg ccaatcatcc agaactgctg taggtaattg
    17581 aaaagaatac tgaataggta caacacggcc aatacgaggg tagtctgacc gatcgtctgg
    17641 aaatccagcg aagcgttggg aacgtgagcg agtttggcgg cgaacccatc gtagagaacg
    17701 gtgatggctt tccccagcac cttaggggag aaaaccgaaa acacgctccc tccgatcgtc
    17761 agaaggatga tcacgaagag tccgaacaag tgggggcgaa ggtacctcac caactttttc
    17821 agagtgccct tcgggtcttt cgctttttga accggtggcc cgaaccgcgc cccgcctcgt
    17881 ccgcccgccc cggggcgagc gcctaaagaa aagttttggg gtaaagaccg gcccttgccc
    17941 gattgcgtgc ccgagctcat gcgatctcct cctcagacaa ctgagaatag acgatttccc
    18001 gatactccgg acagtctcgc atcagctggg cgtgtgatcc ctttcccgct agcacgccgt
    18061 ttttgagcac cagtatttga tccgcgtcca taatagtcga gacccgttga gcgatgatta
    18121 aaagggtttt attccgcatt tccgctcgca acgccaaacg cagtttcgcg tcggttttga
    18181 aatccaaagc ggaaaaagta tcgtcgaaca agagtatcgg cggatcggcc gctatcgccc
    18241 ttgctatcga cagacgctgc ctttgtcctc ccgacaggtc agtgccgcct tgcgcgatca
    18301 cggtatcggc tttttgaggc atcgcgttca cgaagggctc gagctgggcg atccgcatcg
    18361 cccgctcgac ttgcgcgtcg gtgatctctt cacgaccgaa tcggatgttt tctttcacgc
    18421 tccccgtaaa cacgaccgct ttctgcgtag cgtacccgat cttcccgcgg atgtcctcga
    18481 gtttggcctt cttaatgtcc accccgttaa tcataatcga cccctgatct acctcataaa
    18541 accgcatgag cagattgagc agcgtggttt ttcctgaggc ggtgcttccg atgatggcgg
    18601 ttgttttccc gctttcagcg gtgaaagaga tatggtttaa ggccggctcg gaagcgcccg
    18661 gatagcgaaa ggttacctct tcgaaggtga cggtggctcc cggttgatac ggaaggggaa
    18721 cggcttcctc gctgttttcg atggacgccg gtcgagacaa cacctcctcg atacgtctga
    18781 tagaaacctc cgcgcgtggg aggaaggaaa agaccatcga catcataatg aaggcgttcg
    18841 tgatgtgtaa aacgtactga attatcgcca tcagctctcc gatttgcagc gtcccggtat
    18901 cgatttggaa cgatccgaat accaagaggg cgaggatggt gacgttcatc acgagcatga
    18961 tgacgggagt gaccaacgcg acgatccgat ggactcttaa agaggtcgtg gtcaagtcct
    19021 cgttcgtttc gacgaatcgc cgtttttcgt agtcgctttt cccgaaggct cggatgactc
    19081 ggatgcccgt gaggttttct cgtaaaacct ggttcaaacg atccacttta tcctgcatcg
    19141 cacggaagaa cgggatcgcc ttacgggtga tgaaatagac tccaaagagt aaaaacggga
    19201 tggaaacgaa taggatcgat gatagccgcg cgttcttcca aaaggccatg atcacactac
    19261 cgaccgccat aaccggcgcc atcaccacca tgcgcaagga catcataagg gcttgttgga
    19321 tctggctcac gtcgttcgtg gtccgggtga tcaacgaagc gattccaaag ctttccacgt
    19381 cttccaggga aaaggtttcg accttctcga agatcccttt tctgaggtcc cgggcgaaac
    19441 gcgccgaaat ttgcgaggac aaaaaactgg tgcagaagat cacaacaaaa gtaatcgccg
    19501 tcacgcccag catcgccaaa ccggttcgca aaatcagttc gatgtttccg gttgcgatcc
    19561 cacgatcgac gatttttgac atcaagttcg ggaggtacaa ttccgtcatc gcgcgagtga
    19621 ctaccaaaga aatagataag agggcccacc acctgtaggg cttgaaataa agaaacacat
    19681 tcttcacgct taagacccct tttatgcgtt cgcggagatt tgttccagaa ggttttgacg
    19741 tacggttcgt aaaagcgatt gaaggtgcgc ttgctcttcc tgcgtgaacc cgatgacggt
    19801 tatcccatcg atgcttttcc gtaagcgttt cagttgttct acgaccgatt tccctttttc
    19861 ggtcagagaa acggaaaaaa atcggcgatc ggccggatcc gttttcttct ccaccaaccc
    19921 cttggcctcc atcttccgaa gcatcagagt gatcgcggtc ggactgacgc aggccttttc
    19981 ggccaattgg cgttggttcg cgacgcctga tcggtacaag tgcttgagga taggaatttg
    20041 ccgcggagat atctccaagg ctttgaccaa tgcggacata cggatgtggt acaggcgatg
    20101 gatctgaaac agcaattcgc cgatttcgtc atcgaaggcg ctttccaccg gactcctcct
    20161 ataggtactt aattagcaaa ctattaaccc gactttcgac cggttttgga gaaaaaggga
    20221 aaaaataaga aaaaacaa
//
LOCUS       DAOPXN010000019        20154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2693_length_20154_cov_5.162297, whole genome shotgun sequence.
ACCESSION   DAOPXN010000019 DAOPXN010000000
VERSION     DAOPXN010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 20154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20154
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2693_length_20154_cov_5.162297"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1119)
                     /locus_tag="PKM49_02020"
     CDS             complement(139..1119)
                     /locus_tag="PKM49_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNY81625.1"
                     /translation="MKRKKRKRLAINEETLWIPWNGEGERQLTFNETQWIIGKIVEER
                     GSADPEECRRQLLEAKGKLQTILSFTSVLVKVKGGSFQMGNVENDSEEYSEEKPVHTV
                     TLTYDYWIGKYEVTFNECDAYCASTGKSKPSDEEWGRGTRPVMNVSWFDAIGYCNWLS
                     EREGIKKAYDSQGNLLDKSGKVTTDITNVEGHRLPTEAEWEYAARGGQNSRGYKYAGS
                     NDLNEVGWYDGNSDEKTHPVGEKKPNELGLYDMSGNVWEWCHDWYGNYRSAAQTNPTG
                     PSSGAFGAYRVIRGGSWKHNAQYCRAAGRYYYTPTYSYYYLGFRLSRTVF"
     gene            complement(1550..3100)
                     /locus_tag="PKM49_02025"
     CDS             complement(1550..3100)
                     /locus_tag="PKM49_02025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNY81626.1"
                     /translation="MHPRILTVDCGTQSIRVGVIDARGNRVGFEKVSYEPYFSKAPGW
                     AEQDPEIYWNAFCTAARRLKENQPDALENLSGLVVSTLRDTAVAMDEEGRVLRPSIIW
                     LDQRKSTRKIRFSVKDRAIFFLIGMQKTVNTICANAKSNWMIDHEREIWDRAYRYVTI
                     SGYFYFRLTGQWTDSIAAQIGHFPFDYRKHSWDGPSSWKWLGFGIERSKLPQLVEPGA
                     VIGELTPRAAQACGLPKGLPVIAGASDKGCETVGCGCVAPHAASISLGTTATIQITID
                     RYIEPIRFMPPYPAAVKGAYNPEVEIFRGYWMVTWFKREFGTAEVIRAQELGITPEEL
                     LNQRLSEVPPGCHGLLLQPYWSPHVKTPIAKGAMIGFGEVHTRMHIYRAIVEGINYGL
                     IDGMEKIERQSKTPITEIHVSGGGAQSEPICQITADMFDREVFQPAEHETSTLGAAMI
                     GFVGLKIHKDFAQAIREMSRYRRVYHPNPENARIYRDLYNKAYRNIYPRLKHIYRDIK
                     ETIHYPPD"
     gene            3727..4746
                     /gene="ord"
                     /locus_tag="PKM49_02030"
     CDS             3727..4746
                     /gene="ord"
                     /locus_tag="PKM49_02030"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HNY81627.1"
                     /translation="MIRVVCWGLGAMGSGIARNAAKKDQIRVVGGIDTDERLKGKDLG
                     EFLGFKKPMGVKIESDPAKVLNETYPDIVIIATNSFVSNVLPQIMQCVERHINVITIA
                     EEMAYPFESHDEAAQLIDGMAKKYGVTVLGTGINPGFVLDQLILNLTGACLNVKKITA
                     RRINDLSPFGKTVMETQGVGTTPEQFKAGLKNGTIVGHIGFRQSIKMIADYLGWQLDR
                     IEEEREAIISKTHRETPVVKVAPGMVAGCKHIGRGIVGNEVVIELIHPQQIHPEVEKV
                     DTGDYILIEGDPTISMSIKPEIPGGKGTMALATNMIPTVIDWDPGFYSMADLPIPVCL
                     PQKEE"
     gene            4757..5071
                     /gene="ortA"
                     /locus_tag="PKM49_02035"
     CDS             4757..5071
                     /gene="ortA"
                     /locus_tag="PKM49_02035"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HNY81628.1"
                     /translation="MAQTAYKGTWVKISDTILKVGERAPNIPAETAKVPLVMHLNGFL
                     LTDEAQLGDRATIETLSSRIVTGDLIEINPHYTHSFGRFIPELGEIGPHLRKALEKEV
                     EK"
     gene            5068..6465
                     /gene="ortB"
                     /locus_tag="PKM49_02040"
     CDS             5068..6465
                     /gene="ortB"
                     /locus_tag="PKM49_02040"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HNY81629.1"
                     /translation="MTAYQSVMARKNEIMKKAVGIDYDRFELEGCAFDYERMMREVGY
                     SLDEIKGIQQRTRVGRTPLIELRNINRLIKQISPAGKGGRLFLKDEAANPSGSFKDRR
                     ASVSVYQAAKLGYKGVIAATSGNYGAAVASQAAKYGLKCIIVQETFDSRFVGQPEIVE
                     KARACEAFGAEVVQLSVGPELFYYFLVLLEETNYFNASLYTPFGIAGIETLGSEIGEQ
                     MKDLTGEFPDYVVVTHAGGGNLTGTARGLKRVGAERTKFIAASVDLRALHMASDVDFN
                     RKSFTTGHTGFGIPFATFPDRSDVPRNAARPLRYMDDYLMITQGEVFYTTEMLAQLEG
                     LQRGPAGNTSLCAAVALAREVPEGTTLVVQETEYTGAGKCPTAQLTFAKSNGIEVRHG
                     RREDSVPGKNILIPRNPEELGYERVDMDALRASYIKQVIKQYQKTRFSETEIAFLQED
                     CRIDRQTLEHLTEKV"
     gene            6476..8128
                     /locus_tag="PKM49_02045"
     CDS             6476..8128
                     /locus_tag="PKM49_02045"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HNY81630.1"
                     /translation="MATKGTWNQANIRKTNPMFSPFRVTIETPFYGNNVYPVTDIKEA
                     YKMAKNSPGTIVTSLKVKDPERIGLERDTHVLLFNDGTVFGRCAAARRIFGQPGIEVP
                     EFSTILREAAYGTRYRRLYQAEALIGLDHDFMVRARLCVPERHENILYNWLLNFQPIN
                     YEYRQMYAQSRVMEKEGDILVFADPDWSDPRFPLGLSFFDPKANCAAILGMRYFGEYK
                     KGTLTLAWNIANKNGYVSCHGGLKKYALKEKGAFVISFFGLSGSGKSTLTHAKHGGKY
                     DITILHDDAFVISKANGTSIALEPTYFDKTSDYPSDFPDNEFLLTVQNNGATLNEKGE
                     VVLVPEDMRNGNGRAIKSRYWAPNRVDKMSDPIQAVVWLMQDPTIPPLLKINDPVTAS
                     TFGATLATKRTSAERLAEGVDPNALVIEPYANPFRTFPLKDDFLNFKSLFADQGVACY
                     VFNTGFFGSKKVPKEVTLGLLEGIVEKTLTFKPFGPFKQIQIPDVEGFEPRFDDPAYR
                     SEVKKRLEDRARFIEQKKTAAGGYDALPDEAGDVLKQMIQAL"
     gene            complement(8231..13441)
                     /gene="flgK"
                     /locus_tag="PKM49_02050"
     CDS             complement(8231..13441)
                     /gene="flgK"
                     /locus_tag="PKM49_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNY81631.1"
                     /translation="MASYSIFSILNTGVLGTYTSKMAMSVTGHNVANANTEGYSRQRP
                     DIVATPPSATGAFGGSTLNVGTGATVNRVERIRDTFLDRQYREISKNYAYWDEKTRSL
                     HYMEQILNEPSEDGMRSYFDGLWNSFLEVMNQPTNSAAVSSVTATANSFITTAQDLYA
                     RYEDLRANLNQNIRDSADNINLKLQQIADLNGSIRTTYILKTEPNDLLDKRDVILDEL
                     ASMTGMTYKTGLNGHLEIFIGNQSVLYGDIWTPIKATVRPGTLNMYDYFVRSQRIEVT
                     GGTVGALFYMRDVSIPNYLDQMDEMALMLSDKVNLIHRTGYDGLGTVTGIDFFKEMEA
                     VRSELPNLFRTMGTHSILNGPIHMISALTPTDPIMTNLALDGKISLVDLNNKHQLHAQ
                     DSRHFSTNSRFQDVIDYLNEANLYTTRAYNLPQEYDFGLKPEDFPNYKGKAEIYYKTP
                     ERTFTAVNGVLHIGEIEDINNDGMIDFSDIEIKDKNGNRLTNFTYNFVDKIITIHEPF
                     AYTGTVTIRQWESYIGQYRDGELVVPPNEALYKTLSSTAVGTLRLTLDDFVGYTDEAK
                     VFYATQESASFTATNGAFTLPDLVDMDRNDVYDRNDLRIQVNTGSGWVDVANFSYDEP
                     TGRVSILDEAHRSYSGALRVQYWQSERIQLTAAGTFNNAAITGSPVHFGDFDGDIGEL
                     TVNDFHVFLRSEASWEAHVFDGVDKMTLTDYPNFTGFARVFYAISASATATAGAFTLS
                     AASTVPQIDTLIDVNRDGKFDASDIKIQVFSNGNWVDVQRFQYDSSTNTITILDTDLE
                     DFDGDVRFKCWESATVSASSTGVLTLSAITTATCYSAPFSAASTATSEDFKVIAPRER
                     VSDDYQVYLETMRADTMRTTTHDYKLLFGKMSDGLDEYGRVDSAKVNSYGDMREMLVF
                     DQDGLLSRLGYQTVSKEFLKISETLLAQPALTWGFTLDESVDPGEFSLRINKTGEVYQ
                     SSAVITWQGLAEDFKTNEVLKKYFSVTTITDDDGNVRHTIELNKRLSGPEALSILKGA
                     DVLNFQIRNVNPAEIPSENIVWYYDVLDIDEPDATSYSVTFTFPALASPSSPTPVQPI
                     EIEFDDRADLLDQIRKDPILSQYLKIEEKNGQYRLMPTQSINQLQNLEGLEGIETSLK
                     KILSGGAQVLMPSTHLLTTNLEVHYEPKYLTLTVPVFDPISGKTTNTPYTLEFRNRDE
                     LVEKINNNAVLNEYVKVFELDGSYYLTHTPNMENLQTLVTRDEYNILSSKTLKVIDTD
                     AYKTLANILYDYSYAMKKDYTFSSYLSAPPAPPQVTLRLNQNLLEYEGKVEVQYAVRS
                     AGQGGAGFIHNPVGSLRKIDYGLLDIRDINGDGVISKDDIRVLGHTTGNDLEFIFNPR
                     TKEIFLRSNPGENVDIVFWKTDTFNLNDWRRNNTTGHTFSLSEPAVHFEDGVAANVDE
                     AKDFLLRVQLPPDEMNLTLGTSPVTASLYKMTMEGLVDRMNDKAPTGLLSDLTPDGKL
                     VFRAGSGANFSFGRRLDDGRIVQLYDISAPTLFLEKMGFINRNSVFGGSWDFGLDIVR
                     NYLDPNAFMHQENTADNLDVDTRQADGLYAFTKRLSLSSQLGANPQLLAVDYGVWYDR
                     NGDWIADMHSPRGGGNSSSKSIMNLMSEARHSALITDGRTSFNDFYGVFIGEMGIEAE
                     TSSQMHINTQTMKGQITNAREGVKGVSLDEEMSNLIKYQHAFTAAARIITAVDEMIQT
                     VVSRLGLVGR"
     gene            complement(13726..14622)
                     /gene="flgL"
                     /locus_tag="PKM49_02055"
     CDS             complement(13726..14622)
                     /gene="flgL"
                     /locus_tag="PKM49_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HNY81632.1"
                     /translation="MRITHNMLHNTVLKDITDTYREVAKLHEQGSSGKRVRYPSDDAI
                     VATRASNIGTKLREIEQYQRNASTVDTYIKHYDSITDELGALAYRLRELVVNGANGTL
                     TVHDRSTIAEEIDRIKDQFIQLANTQVSGEYIFAGAEARVAPVNADGKIVMSAEAHVS
                     KTVDLGRYRFEYNFTVFDAFTVNGNESIFHLMDDLSKNLREDNPKEYLNTHALEKLDR
                     YEANLQKLIARNGANGNFVQMATKRFDDSVVFMTEYLSKEQDADFMETYTNLSNLEAV
                     LQAAMKTGGNIMNYSLVDYVRG"
     gene            complement(14636..15124)
                     /gene="flgN"
                     /locus_tag="PKM49_02060"
     CDS             complement(14636..15124)
                     /gene="flgN"
                     /locus_tag="PKM49_02060"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HNY81633.1"
                     /translation="MVQEVRELIEVFSEFENTVRENIELLKEQQQTMIDGEYDLFEEL
                     TFKQEEFDRKLRIIEQRRMTLSDSLCEALSLPEGSSILQIVERLGDEGRDLMISVTQM
                     MEAIQELTFSRANLERMVQFQLSYIDFLQAGMSGTRKMQTYTPAGKSSNLRKTERFQG
                     DG"
     gene            complement(15139..15429)
                     /locus_tag="PKM49_02065"
     CDS             complement(15139..15429)
                     /locus_tag="PKM49_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNY81634.1"
                     /translation="MIDPKDILRIGDVQKAYPTVRKRVKGESEPVRQEEESLFSSERL
                     TIAGTTSLSEILKKAELLPEVREELVSKFQKLIESGAYKPDPDRIARKILEG"
     gene            complement(15499..17892)
                     /gene="recG"
                     /locus_tag="PKM49_02070"
     CDS             complement(15499..17892)
                     /gene="recG"
                     /locus_tag="PKM49_02070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNY81635.1"
                     /translation="MILIEEFISQSEGLLKKAREDEASKASLHRRLLSTLNQIDKLEW
                     ETAGEEGEKLRLFVSHYDRFDTLPADRQEKRLANGFAMLDKLKAIFLFPPPLETPSES
                     ESQKLKELLQRPVQYVKGVGPRWTDFFNSVDVQTLKDLFFFFPRTYHDRRRVYRVSEL
                     FPNIKATVFGTLGPVMEKRTNYGLHILTVTLNDGTGEVALIWYNQPFLKEKLSKTNRL
                     MVTGNVRWTRQRGWEIEVQDYEEEQPEDRPTRTIVPVYRSRKGFPSKSIRKVILNTSY
                     ALAAIEDPVPTSINEKRRLIPLADAIYGLHQPKSQAHLQQCRRKMSYNEFFYFQTALL
                     FARRNREEFYTRPAKRIEGTLSERLVKGLPFKLTKAQKRVYQSIRAKLQNNLPVNVLL
                     QGDVGCGKTIVAEMVLLDIIESGFQAAFMAPTAILAQQHFRTIADDFARLDIQPRLLI
                     SGLPNKEKELVKEEIRSGHAPLVIGTHALIQEDVVFSRLGLALVDEQHRFGVKQRESL
                     KSKGEQVDTLIMSATPIPRTLALAVYGDLDVETIDETPPGRKAVRTVYIHESKKGEVY
                     EIVRQEVASGNQAFLVFPIIDEAESEDSPAADLKAAVKMFEVLSKGPFKGIPLGLIHG
                     RLKEEEKDAVMGRFVSGEVKVLVATTVIEVGIDVPKATVMVIENPERFGLAQLHQLRG
                     RVGRSERHSYCFLVTNDTSEASEKRLKYFSGCNDGFQLAEYDLQTRGPGDFLGVRQHG
                     MPRFEFGDMVEDANLLYQCREDAQELLSEDIALEKNPKLRDFLLHEYSDKIDRIQTG"
     gene            complement(17894..18514)
                     /locus_tag="PKM49_02075"
     CDS             complement(17894..18514)
                     /locus_tag="PKM49_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNY81636.1"
                     /translation="MKSVSAFGEALRKLRVEHSENQTELGQVLGVSPQMVSKFETGKS
                     VPSAITLKKLAEHYRVSIETLLGLERAIPTGEELVRRLPVLGKISAGKPLYAEEQQIG
                     TYPVLPGVEGDFLLLVTGDSMVPVIPDQSYVVVKCDLPVSSGGIAVVMVPDTEAVVKR
                     VYYDPNGVILKSENSAYPPLFFDKERWASLCRMIGKVTTVIHRLVE"
     gene            18720..18956
                     /locus_tag="PKM49_02080"
     CDS             18720..18956
                     /locus_tag="PKM49_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HNY81637.1"
                     /translation="MGLNDPVNVDLTQKVAVLILDTGVLKNEKPVLKRIRFPVLSTAT
                     NQQVYDAAYALAEYTEYSVYDIEIQNMDSLDPID"
     gene            18975..19184
                     /locus_tag="PKM49_02085"
     CDS             18975..19184
                     /locus_tag="PKM49_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81638.1"
                     /translation="MITLELNFKNAISGKSKRLSIPEPVDNIDELTEALDTHMVTYVQ
                     PVLSGSLVFASARLVNREITELIEK"
     gene            19203..19457
                     /locus_tag="PKM49_02090"
     CDS             19203..19457
                     /locus_tag="PKM49_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81639.1"
                     /translation="MKKAALNLPAILKMICTLAALVVEEPGKGAEKKQKAIQLVHEFI
                     LSMGIHIPKAVLDLVLPPMIDQVVGILNQTVWLTPTTLVR"
     gene            19965..>20154
                     /locus_tag="PKM49_02095"
     CDS             19965..>20154
                     /locus_tag="PKM49_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY81640.1"
                     /translation="MEKFYNPHDIFFKEVLGDVVNTQAFLETYLPYEVLQTIDVGTIQ
                     AEKDSFIEQDLKEFYADLL"
BASE COUNT         4435 a         5575 c         5469 g         4675 t
ORIGIN      
        1 ccctctttag aagggcgaag aagggatcgc ggggtccctt cggttacccc gcgatgacct
       61 ccgagattcg ctccaccctt ttcggggaaa aacacaaagt caaaaccagc aaaaggtaaa
      121 aaggtaaaaa tacaaaagtt aaaagaccgt cctggaaagg cgaaagccga ggtagtagta
      181 gctgtaagtc ggcgtgtagt agtaccgacc cgccgctcgg cagtactgcg cgttgtgttt
      241 ccagctgccg ccccgaatca cccggtaagc gccgaacgcg ccactactcg gccctgtcgg
      301 gtttgtttgc gcggcgcttc tgtaatttcc ataccagtcg tgacaccatt cccacacgtt
      361 cccgctcata tcgtagagcc ccagttcgtt cggtttcttc tcgcctaccg ggtgcgtctt
      421 ctcatcagaa ttgccgtcgt accaccccac ttcgtttagg tcgttgcttc ctgcgtattt
      481 ataccccctt gagttttgtc cccctcgcgc cgcgtattcc cattccgctt ccgtgggcaa
      541 tcgatgccct tctacgttcg tgatgtctgt cgttaccttt ccgctcttgt ccagcaagtt
      601 tccctggcta tcgtacgctt tctttatccc ttctctctca ctcagccagt tgcagtaccc
      661 gatcgcgtcg aaccaactca cattcatcac cggccgcgtc ccgcggcccc attcttcgtc
      721 gcttggcttg ctcttccccg tcgacgcaca gtacgcgtcg cactcattga acgtcacttc
      781 gtacttcccg atccagtagt cgtacgtcag ggtcaccgtg tgtaccggct tttcttcgct
      841 gtactcttcg ctgtcatttt ccacattccc catctggaag ctcccgcctt tgactttgac
      901 cagaaccgat gtgaacgaaa gaatcgtttg aagctttcct ttcgcctcca gcaactgtcg
      961 acggcactct tccgggtccg cgctgccccg ctcttcgaca atttttccga tgatccactg
     1021 cgtttcatta aaggtgagct gccgctcccc ttcaccattc cacggtatcc ataacgtctc
     1081 ttcgtttatc gcaagcctct tccttttctt tctcttcatt tctcctccga taacagatta
     1141 ctcctcgtgg cgtaagcgcc ccttcttttt gcgacggcgc gatacgattt tgttcgatcc
     1201 ttgttcccgt tttagttact tgctaatgca cgcttactac caaagatagg atcgcaagac
     1261 ttcgtgaact cccgaagtca gcccgctggt taccccgcga taacctataa agtccggcgt
     1321 tctcgagcgc agcatcgcgg cgtaagcgcc ccttcttttt gcgacggcgc gatacgcttt
     1381 tgtacacgtc ttgaaaaaca gggttcgcac ggagggcaca gagaacacag aggaaaaact
     1441 ggcaaacaac agcattctcc cggaaaaaga aaagaaatgc cggacaaaag tccggcgctc
     1501 ccaaggtgcc ccagggaaac gttccggtac gaaagccgcc gagttggggc taatccgggg
     1561 ggtagtggat cgtttctttg atatcccgat agatatgctt caacctcggg tagatgttgc
     1621 ggtaggcttt gttgtataag tcgcggtaga tgcgcgcgtt ttctgggttc gggtggtaga
     1681 cccgtcggta gcgggacatc tctcggatcg cttgcgcaaa gtctttgtgg atcttgagcc
     1741 cgacaaaccc gatcatcgcg gctcctaagg tagaggtttc gtgttcggcc ggctgaaaga
     1801 cctctcggtc gaacatatcg gcggtgattt ggcagattgg ctcgctttgg gcgcctccgc
     1861 cggacacgtg gatttcggtg ataggggttt ttgactggcg ctcgatcttt tccatcccgt
     1921 ctattaaccc gtaattgatt ccttcgacga tcgcgcggta aatgtgcata cgcgtgtgta
     1981 cctccccgaa accgatcatc gcccccttcg caatcggcgt tttcacgtgg ggactccaat
     2041 agggctggag gagcaatccg tggcatccgg gcggcacctc gctcaaccgc tgattcaaca
     2101 gctcttccgg ggtgatgccg agttcttgcg cgcgtatgac ctccgccgtg ccgaactcgc
     2161 gcttgaacca ggtgaccatc caatacccgc ggaatatttc gacttccggg ttgtaggccc
     2221 ccttcaccgc ggccgggtag gggggcataa accgaatggg ctcgatatac cggtcgatgg
     2281 tgatctgtat cgtggcggtc gttcccaagc tgatcgaggc ggcgtgcggg gcgacgcatc
     2341 cgcaaccgac cgtttcgcac cccttgtccg aggcgcccgc gataacgggc aagcctttgg
     2401 gcaatccgca ggcctgcgcg gctcgggggg tcaactcccc gatgacggcg cccggctcaa
     2461 ccaactgcgg cagtttcgac cgttcgatcc cgaacccgag ccatttccaa ctgctcgggc
     2521 catcccaact gtgttttcgg tagtcaaacg ggaaatggcc gatctgcgcg gcaatcgaat
     2581 ctgtccattg ccctgtgagt cgaaagtaga aatatccgga gatcgtgacg tagcgatagg
     2641 cgcggtccca gatctccctc tcgtggtcta tcatccaatt ggacttggcg ttcgcgcaga
     2701 tcgtgttgac ggtcttttgc ataccaatga gaaagaaaat cgcgcggtcc ttcacagaaa
     2761 accggatttt tcgggtggat tttctctggt ccagccagat aatcgacggg cgtaggacac
     2821 ggccctcttc gtccatagcc acagccgtgt ccctcaatgt ggaaactacg agaccagaaa
     2881 ggttttcgag cgcgtcgggc tggttttctt tgaggcggcg cgccgcggtg caaaaggcat
     2941 tccaataaat ctccgggtct tgttccgccc aacccggcgc cttagaaaag tagggttcgt
     3001 acgagacttt ttcaaaaccg acgcgatttc ctctcgcgtc tatgacacca actcgaatgc
     3061 tttgcgtgcc gcagtccacg gtcaaaatgc gcggatgcac cctatcacct cacctcgaaa
     3121 actcccttgt ggcgattata cgtgtgggtc aagttatggg atgacataac ctatttcaag
     3181 acatgccaac ttaaaagttg gcgttcccag gcggccacgc cggattttgt gcggcacggg
     3241 gttttgcttc ttgggaacgc cacactttag tgtggcatgg gttgtgtttt tttactttga
     3301 aaacccaaaa catgccaact taaaagttgg cgttcccagg ggtatcgcgc cgtcgcgaaa
     3361 aaaaggggcg cttacgccgc gatgccttcg aaaaacccaa aacatgccaa cttaaaagtt
     3421 ggcgttccca gggcgttccc aagcgttccc agtgttccca gggcgttccc gaacttatcg
     3481 cgccatcaat tctggccttg tttcttgatt actccgcata gcttgaccca cacgattata
     3541 acctaaacgg cgatttctat tatatactct cgtataagcg cataagaagg aggaaaagcg
     3601 cgataaaccc cgaaatgcgg aattaagtga aattggtcag gaaatgcaac cattgaaaat
     3661 tgtgatagaa tcccttaaag aggtaatatc gcatcgacaa tcatcgcgca gcataggagg
     3721 gaaaacatga tacgagtggt ttgttgggga ttaggcgcta tgggttctgg catagcgaga
     3781 aatgcagcga agaaggatca gatccgcgtg gttggcggga tcgacaccga tgaaaggctg
     3841 aagggaaagg acctcggcga attcctaggg tttaagaaac cgatgggcgt gaaaatcgaa
     3901 tccgatccgg cgaaggttct gaacgaaacc tatccggata tcgtgatcat cgcgacgaat
     3961 tcgttcgtca gcaacgttct accgcaaatc atgcagtgtg tggaaaggca catcaacgtc
     4021 atcaccatcg cggaggagat ggcgtacccc tttgaaagcc acgacgaagc ggcgcagctg
     4081 atcgacggta tggcgaaaaa gtacggggtg accgtgttgg gaaccggaat caacccgggc
     4141 ttcgtgctgg accagttgat attgaatctg accggcgcgt gtttgaacgt gaagaagatt
     4201 acggcaagga ggatcaacga cctctccccg ttcgggaaga ccgttatgga gactcaagga
     4261 gtgggaacga caccggagca gttcaaagcg gggctaaaaa acggaaccat cgtggggcac
     4321 atcgggttcc ggcaatccat caagatgatc gccgattatt tgggctggca gctcgaccgg
     4381 atcgaagaag aacgcgaagc cattatctcg aaaacccatc gagaaacgcc ggttgtgaag
     4441 gtagcgcccg gaatggtagc cggttgcaaa catattggac gggggatcgt ggggaatgaa
     4501 gtggtgatcg agctcatcca cccccaacaa atccaccccg aagtcgagaa ggtcgacacc
     4561 ggcgactata tcctgatcga gggcgacccg acaatttcga tgtcgataaa accagaaatc
     4621 ccaggcggaa aaggaacgat ggccttggcg acgaatatga tccccaccgt gatcgattgg
     4681 gaccctggat tctattcgat ggccgatctc ccgataccgg tctgtctccc ccaaaaagaa
     4741 gagtgaatgc agggctatgg cacagacagc ctataaggga acgtgggtga aaatatcgga
     4801 caccatcttg aaggtcgggg aacgggctcc gaatattccc gcagaaacgg cgaaggtgcc
     4861 gctggtaatg catctgaacg gcttccttct gacggatgaa gcgcagctgg gcgaccgcgc
     4921 cacgatcgaa accctgtcga gccgaatagt gaccggcgac ttgatcgaga tcaacccgca
     4981 ctacacgcac tccttcggtc ggttcattcc cgagttggga gagattggac cccatctgcg
     5041 caaagcgctg gagaaggagg tggagaaatg accgcctacc aatccgttat ggctcgaaag
     5101 aacgaaatta tgaaaaaggc ggtcgggatc gactacgacc ggtttgagct cgaaggttgc
     5161 gccttcgact atgaaaggat gatgcgggag gtggggtact ctctcgacga aatcaaggga
     5221 atccaacagc gaacccgcgt cgggcgaacc ccgctcatcg agttaaggaa catcaaccgt
     5281 ctcataaagc agattagccc cgcagggaag ggtgggcgcc tgttcctgaa agacgaggcg
     5341 gcgaaccctt ccggaagttt caaagacagg cgagcctccg tttccgttta tcaagcggcg
     5401 aaattgggat acaagggtgt gatcgccgcg acaagtggga actacggcgc ggcagtggcg
     5461 tcacaagccg cgaaatacgg gttaaaatgc atcatcgtcc aagagacctt cgacagccgg
     5521 tttgtgggcc agccggagat cgtggaaaag gcgcgagcct gcgaagcctt cggggcggaa
     5581 gtcgtgcagt tatccgtagg ccccgagctc ttctactatt tcttggtctt gctggaggaa
     5641 acgaactact tcaacgcctc gttatacacg ccctttggca tcgccggtat cgagaccttg
     5701 ggctcggaga taggcgagca aatgaaagat ctgaccggag aattcccgga ctacgtcgtg
     5761 gtcacgcacg cgggcggcgg caacctgacg ggtaccgcgc gtgggttaaa gcgcgtcggc
     5821 gcggagcgga cgaagtttat cgccgcgagc gtcgatctgc gagcgctgca tatggccagc
     5881 gacgtcgatt tcaaccggaa gtccttcaca acgggccata cggggtttgg cattccgttc
     5941 gccaccttcc ccgatcgttc ggacgtaccg cggaacgcgg cccgaccgct ccgctatatg
     6001 gacgattacc tgatgatcac acaaggggag gtcttctaca ccacagagat gctcgcgcag
     6061 ttggaagggc tgcagcgcgg tccggcgggc aacacctcct tgtgcgcggc ggtcgcgttg
     6121 gcgcgggagg tgccggaagg aaccacgctc gtcgtacaag agaccgagta caccggcgcg
     6181 ggaaaatgcc ccacggcgca gttgaccttt gcgaaatcca atgggatcga agtgaggcac
     6241 ggccgccgag aggacagcgt acccggaaaa aacatcctga tcccccgcaa ccccgaggag
     6301 ctagggtacg aacgggtgga tatggacgcg ctcagggcct cgtatatcaa gcaggtcatc
     6361 aaacagtatc aaaaaacgcg tttctcagaa actgaaatag cattcttgca ggaagactgc
     6421 cggatcgacc ggcaaacact cgaacatttg accgaaaaag tataaggagg gagtaatggc
     6481 aacgaaggga acttggaacc aagcgaatat ccgaaagacc aatccgatgt tttctccctt
     6541 tcgggtcacg atcgaaaccc cgttttacgg gaacaatgtc tacccagtta ccgacatcaa
     6601 agaagcctat aagatggcga aaaactctcc aggaacgatc gtcacgtctt tgaaagtgaa
     6661 ggatccggaa aggatcgggt tggagagaga cactcacgtc ttgttgttca acgacggcac
     6721 cgtttttggg cgctgcgcgg cggcgcgccg tatcttcggg caacccggca tagaggtccc
     6781 cgagttttcg acgatcttac gggaagccgc atacggaacc cgttatcgtc gcctttacca
     6841 agcggaggcc ctgatcgggc tggatcacga tttcatggtg cgggctcgac tctgcgtacc
     6901 ggaaagacac gaaaacatcc tgtacaactg gcttttaaac ttccaaccga tcaactacga
     6961 ataccgtcag atgtacgcgc aatctcgtgt gatggaaaag gaaggcgaca tcctcgtctt
     7021 cgccgacccc gattggtctg acccgcgatt cccactcggg ttgagcttct tcgacccgaa
     7081 ggcgaactgc gcagccatct tgggtatgcg ctatttcggg gagtacaaga aaggcacgct
     7141 gaccttggcg tggaatatcg cgaacaaaaa cggatacgtg tcctgccacg gtgggttgaa
     7201 aaagtacgct ctgaaagaga aaggcgcctt cgtcatctcg tttttcgggc tttccggatc
     7261 cgggaaatcg acgctcacgc acgccaaaca cggcggtaaa tatgacatca ccatcctgca
     7321 cgacgacgcg ttcgtcatat cgaaagcgaa cggcacgtcg atcgcgttgg agcccacgta
     7381 tttcgacaaa acctcggact atccgagtga cttccccgac aacgagttcc tgctcaccgt
     7441 ccaaaataac ggcgccacgc tgaacgaaaa gggcgaggtc gtgctggtcc ccgaagatat
     7501 gcggaacggg aacggccgag cgatcaaatc ccggtactgg gcgccgaatc gtgtggataa
     7561 gatgagcgat ccgattcaag cggtcgtgtg gttgatgcaa gaccccacga tccccccact
     7621 tctgaagatc aacgatcccg taaccgcctc gaccttcggc gctacgctcg cgacgaaacg
     7681 cacctcggcg gagaggttgg cggaaggtgt ggaccccaac gccctcgtta tcgagcccta
     7741 cgcgaacccg ttccgaacct tcccgctgaa ggacgacttc ctcaatttca agtccttatt
     7801 cgccgaccaa ggagtggcgt gctacgtctt caacaccggc ttcttcggat cgaaaaaggt
     7861 cccgaaagag gtgacgctgg gcttgctgga gggtatcgtg gaaaagacgc tgaccttcaa
     7921 accattcggg ccgttcaaac agatacaaat cccggatgtg gagggtttcg agccccggtt
     7981 cgacgatccg gcttaccggt ccgaggtcaa aaaacgcttg gaagaccgag cgcgatttat
     8041 cgaacagaag aaaacggccg cgggcgggta tgacgcgctg ccggacgagg cgggcgacgt
     8101 cctcaaacag atgatccaag cgctgtaacc cgcgcttgac cacaaatgaa aaaagaggcg
     8161 gagtacgcct ctttttttga tctcgcgttc agcttgcgcg agcgtgttat gggctgatag
     8221 ctggtggcgt ttaccgaccg acgagcccga gacgcgagac gaccgtctgg atcatctcgt
     8281 cgacggcggt gatgatgcgt gcggcggcgg tgaaggcgtg ttgatattta atcaggttag
     8341 acatctcctc gtcgagggag acgcctttca caccctctcg ggcattcgta atctgcccct
     8401 tcatcgtctg ggtgttgata tgcatctgag acgaggtctc cgcttcgatc cccatctccc
     8461 cgatgaagac accgtagaaa tcgttaaagg acgtccggcc atccgtgatg agggccgaat
     8521 ggcgggcttc gctcatcagg ttcataatcg acttcgacga actgtttccc ccgccccgtg
     8581 gagaatgcat atcggcgatc caatccccgt tacggtcata ccatacgccg tagtcgacgg
     8641 ccaacagctg aggattggcg cccagctggc tggacaaaga caagcgtttg gtgaaagcat
     8701 agagcccgtc ggcttgacgc gtgtcgacat cgaggttgtc cgccgtgttc tcttgatgca
     8761 taaaggcgtt cgggtcgaga taattccgga cgatatccaa accgaagtcc catgagcccc
     8821 cgaagaccga gttcctgttg atgaagccca tcttttccag aaagagagtg ggcgcggaaa
     8881 tatcgtacaa ttggacaatt cgcccgtcat ccaatcgcct tccgaagctg aaattcgcgc
     8941 cgcttccggc acgaaaaacc agtttcccgt ccggcgtcag gtccgacagc aggcccgtcg
     9001 gcgctttatc gttcatcctg tcgaccaagc cctccatcgt catcttgtag aggctcgccg
     9061 ttaccggaga agtcccgagc gtgaggttca tctcgtcggg aggaagttga acccggagca
     9121 agaagtcctt ggcttcgtcc acgttggccg caactccgtc ctcgaagtgc acggcgggct
     9181 cggataggga gaaggtgtgg ccggtcgtat tgttccttcg ccagtcgttg agattgaaag
     9241 tatcagtttt ccagaacacg atatcgacgt tctcgcccgg attcgatcgc aaaaaaatct
     9301 ctttggtccg tgggttgaag ataaattcga gatcatttcc cgtggtatgg cctaacacac
     9361 gaatgtcgtc ttttgaaatg accccgtccc cgttgatatc ccgaatatcg agtaggccat
     9421 aatcgatctt gcgaagcgat cccacaggat tgtggataaa tcctgcgccc ccctgcccgg
     9481 cgcttctcac ggcgtactgg acctccactt tcccttcgta ctccaaaagg ttttgattca
     9541 gcctgagagt tacttgagga ggcgcaggcg gagctgacaa gtaactcgag aaagtgtaat
     9601 ccttcttcat cgcgtacgaa taatcgtaca ggatgttggc caacgttttg taggcgtccg
     9661 tgtcgatcac cttcagcgtt tttgaggaga ggatattgta ttcgtcgcgc gtgacgaggg
     9721 tttgcaagtt ttccatattc ggggtgtggg tcaggtagta ggagccgtca agctcgaaca
     9781 ccttcacgta ttcgttcagc accgcgttgt tgttgatctt ttcgaccaac tcgtctcggt
     9841 tccggaattc gagcgtgtaa ggggtgttcg tcgtcttccc cgaaattgga tcgaagacgg
     9901 gaactgtcaa agtcaggtat tttggctcgt aatggacctc cagattggtc gtcagtaagt
     9961 gagttgacgg catcaacacc tgagcgcctc ctgaaaggat ctttttcaaa gaggtttcga
    10021 taccctccaa tccctccagg ttctgcaact ggtttatgga ctgtgtaggc atcagccggt
    10081 actggccgtt cttctcttcg atcttcaagt attggcttag aatcggatct ttacgtattt
    10141 gatcaaggag gtccgcgcgg tcatcaaact cgatctcgat cggttgaacc ggtgtcggcg
    10201 aggagggtga ggccagcgcg ggaaaggtga acgtgaccga gtaactcgta gcgtcaggtt
    10261 cgtcgatgtc caacacgtcg tagtaccaca cgatgttctc ggaagggatt tcggcggggt
    10321 tcacgttgcg tatctggaag ttcaggacat ccgcgccttt cagaatcgat agcgcctccg
    10381 ggccggaaag gcgtttgttc agctctatcg tatgtctgac attgccgtcg tcgtcggtta
    10441 tcgtcgtcac agagaaatac tttttcaaga cttcgttggt cttgaagtct tcggccaatc
    10501 cctgccaggt aatcactgcc gaactctggt aaacttcgcc cgttttattt atgcgcaaag
    10561 agaactcccc ggggtcaacg gattcatcca acgtgaaccc ccaggtgagc gccggttggg
    10621 ccaacagcgt ctcggagatc ttcaggaatt ctttggaaac cgtttgatag cccaaacggc
    10681 ttaagagtcc atcctggtcg aagaccagca tctcgcgcat atccccgtag ctgttgactt
    10741 tcgcgctatc cacccgcccg tattcgtcga gtccgtcgct catctttccg aacaggagct
    10801 tgtagtcgtg agtggtcgtg cgcatcgtgt ccgcgcgcat cgtttcgaga tagacctggt
    10861 aatcgtcgct aaccctctca cggggggcga tcaccttgaa atcctcgctc gtcgccgtag
    10921 aggcggccga aaaaggcgcg gaatagcacg tcgcagtcgt aatcgccgat agggtcagaa
    10981 cacccgtaga actggcagat accgtggcgc tctcccagca tttgaaacga acgtcgccgt
    11041 cgaaatcttc taagtcggta tcgagtatgg tgattgtgtt agtagaggaa tcgtattgaa
    11101 atcgctggac atccacccaa ttgccgttgg aaaacacttg tatctttata tcggacgcgt
    11161 cgaacttccc gtcgcggttg acgtcgatta aagtgtcgat ttgcgggacg gtcgacgcgg
    11221 cgctcaacgt gaaagcccct gcggtggccg tggcggaagc cgagatcgcg taaaagacgc
    11281 gcgcgaaccc cgtgaagttc ggatagtccg ttagggtcat tttatctaca ccgtcaaaaa
    11341 cgtgcgcctc ccaagaggcc tccgaacgca agaacacgtg gaaatcattc acggtcaatt
    11401 ctccgatgtc gccatcgaaa tccccaaagt gaacgggact acccgttatc gccgcgttgt
    11461 tgaaggtccc cgcggcggtc agctgtatcc gctcgctctg ccaatactga actctcagcg
    11521 cgccggagta ggatctgtgc gcctcgtcga gaatagaaac gcgtcccgtc ggttcgtcgt
    11581 aggaaaaatt ggccacatcc acccaacccg agccagtgtt cacttggatg cgcaggtcat
    11641 tgcgatcgta cacatcgttt ctgtccatat cgacgaggtc gggaagtgtg aaggcgccgt
    11701 tggtcgccgt gaagctcgcc gactcctgcg tcgcgtagaa gaccttcgcc tcatcggtat
    11761 acccgacgaa gtcgtctaaa gttaatctca aagtgccgac cgccgtggaa gagagggttt
    11821 tatacaacgc ctcgttcggt ggcaccacca attccccgtc ccgatactgg ccgatgtagg
    11881 actcccattg gcggatggtg acggtgccgg tgtaggcgaa gggttcgtgg atcgtgatga
    11941 ttttgtccac gaagttgtag gtaaaattcg tcagtcggtt cccattttta tccttgatct
    12001 cgatgtccga gaaatcgatc ataccgtcgt tgttgatgtc ttcgatctct ccgatgtgca
    12061 ggacgccgtt caccgccgtg aatgtcctct ccggggtttt ataatagatt tcggccttcc
    12121 ccttgtagtt agggaagtct tccggtttca acccgaaatc gtactcctgt ggaaggttgt
    12181 aggcccgcgt agtgtagaga ttcgcttcgt tcaggtagtc gatcacgtcc tgaaaccgag
    12241 aattcgtgga aaaatgccga ctgtcttgcg cgtggagttg gtgcttatta ttcaagtcca
    12301 ccaaggagat tttcccgtcg agcgcgaggt tggtcattat gggatcggtc ggcgttagtg
    12361 cggatatcat atggatcggg ccgttgagga tcgaatgggt gcccatcgtc cgaaagaggt
    12421 tcggcagctc ggagcggacg gcctccatct ctttgaagaa gtcgatcccc gtcaccgttc
    12481 caagcccgtc gtatccggtc cgatggatca ggttcacctt gtcagacaac atcagggcca
    12541 tctcatccat ctggtccaga tagtttggaa tcgatacgtc gcgcatataa aacagcgccc
    12601 cgaccgtacc gcctgtgacc tcgatccgtt gggagcgaac gaaatagtcg tacatgttca
    12661 gcgtccccgg tcgcaccgtc gctttgatcg gcgtccagat atcgccgtac aacaccgatt
    12721 ggtttcctat aaatatctcc agatggccgt ttaaaccggt tttataggtc atcccggtca
    12781 tcgaggccag ttcgtccaaa atcacgtccc gcttgtctaa aagatcgttc ggctcggttt
    12841 tcaggatgta cgtggtccgg atcgacccgt tcagatcggc gatctgctgt aatttgaggt
    12901 tgatgttgtc ggcagagtcc cgaatgtttt ggttgaggtt cgcccggagg tcttcgtatc
    12961 gggcgtacag atcctgggcc gtcgtgatga agctgttggc tgtggccgtc accgagctga
    13021 ccgcggcgga gttcgtcggc tggttcatca cttcgaggaa agaattccac aacccgtcga
    13081 agtaactccg catcccgtct tcactgggtt cgttcaatat ttgttccata tagtggaggc
    13141 tgcgggtctt ctcgtcccaa taggcgtagt ttttcgatat ttcgcggtac tgtcgatcca
    13201 aaaaggtgtc ccgtatccgc tcgacccgat tcaccgtcgc gcccgtgccg acgtttaacg
    13261 tcgacccacc gaaagcgccg gtcgcggagg gcggggtggc gacgatatcc ggtctttgcc
    13321 gcgaataccc ctccgtgttc gcgttggcga cgttgtggcc ggtgacgctc atcgccatct
    13381 tcgaggtgta ggttcccaat acccctgtgt tcaatatcga aaaaatgctg taactagcca
    13441 tattgacctc ccgcgcttct taggtgtgtt tttatcggaa tccgagggcg attccttaat
    13501 aggacaagcc tcgataccct ctcgaggagc caaaaacaga aagggggtgg tctctgtcca
    13561 ccgccttagg ccagaacccg taggacgtgg acgaacgaac accgatctcg cggagacggg
    13621 ctttccgacc cgatcggcga aacagaacgg cggaggaatg ggctctagaa gggtaaggac
    13681 tatgaatgat gtcggcttta ggatcgacgc gtttcgcccg cgcgattacc ctcggacgta
    13741 gtcgaccaga gaatagttca tgatgttacc tccggtcttc atcgccgcct gcagcaccgc
    13801 ctctaggttg gacaggttcg tgtaggtctc catgaagtcc gcgtcttgct ctttggaaag
    13861 gtattcggtc ataaagacga cggaatcgtc aaaccgtttt gtggccatct gaacgaagtt
    13921 gccgttcgcc ccgtttcggg cgatcagttt ctgtaagttg gcttcgtacc gatcgagctt
    13981 ttccagcgca tgggtgttca ggtactcctt cgggttgtct tcccgaaggt tcttgctgag
    14041 atcgtccatc agatgaaaga tactttcgtt gccgttcacc gtgaaggcgt cgaagacggt
    14101 gaagttgtat tcaaaacggt accttcccag gtctacggtt ttggaaacgt gagcttcagc
    14161 cgacatcacg atctttccgt cggcgttgac cggggccacc cgcgcttccg cgccggcgaa
    14221 gatgtactcc ccggagactt gggtgttcgc cagctggatg aactggtcct tgatccggtc
    14281 gatctcttcg gcgatcgtcg agcggtcgtg aacggtcagg gtgccgttcg cgccgttcac
    14341 gaccagttct cgcaggcgat aagctaaagc gcccaattcg tccgtgatac tgtcgtagtg
    14401 cttgatgtag gtgtccaccg tggaggcgtt tcgctgatac tgttcgatct cccggagttt
    14461 ggtgccgatg ttggacgccc tcgtcgcgac aatcgcgtcg tcggaggggt agcgcacccg
    14521 ctttccggaa gacccttgct cgtggagctt cgccacctct cgatacgtgt cggtgatgtc
    14581 cttcaacact gtattatgta acatgttatg cgttattctc aatgtcctgt ccctcctatc
    14641 cgtcgccttg aaaccgctcg gtcttccgca gattggaaga ttttccggcg ggggtgtagg
    14701 tctgcatctt tctcgttccg gacattccgg cctggaggaa gtcgatgtag gagagctgaa
    14761 attgaaccat ccgctcgaga ttcgcgcgtg agaaggtcag ctcctgtatc gcctccatca
    14821 tctgtgtcac gctgatcatc aggtctcgcc cctcgtcgcc caaccgttcc acgatctgca
    14881 ggatagacga gccctcgggg agcgacaacg cctcgcatag ggaatcggag agggtcatcc
    14941 gtcgctgttc tatgattcgg agcttgcggt cgaactcctc ttgcttgaag gtcagctctt
    15001 cgaacagatc gtattcgccg tcgatcatcg tctgctgctg ttctttcaac agctcgatgt
    15061 tctcgcgaac cgtgttctcg aattcggaga agacctcgat gagctcccgt acctcttgaa
    15121 ccattcagaa tcctccggtc acccttcgag tatctttctc gcgattcgat caggatcggg
    15181 tttgtacgcg ccgctctcga tgagtttctg aaacttcgaa acgagttctt cccgcacctc
    15241 gggcagcagc tccgcttttt tcagaatctc ggaaaggctg gtggttccgg ctatcgtcaa
    15301 gcgctccgag gagaacaggc tttcctcttc ttggcgaaca ggttccgatt cgcctttcac
    15361 gcgtttgcgg accgtcgggt acgccttttg cacgtcgcct attctgagta tgtctttggg
    15421 atcgatcatc accattcacc cctattacca gtatgtccat ctgtttatcg gatcgggcct
    15481 tcgaaacttt aataatcgtt aaccggtctg tatgcggtcg atcttatcgg aatactcgtg
    15541 gagtaaaaaa tcgcgtagct tcgggttctt ctccaaagcg atgtcttccg aaagaagctc
    15601 ctgcgcgtct tctcgacact gataaagcag attggcgtcc tctaccatgt caccaaattc
    15661 gaaccgtggc atcccgtgtt gccggacgcc caagaaatct cctggcccgc gggtctgcaa
    15721 atcgtactcg gccagttgaa acccatcgtt gcaaccggag aaatatttta accgtttctc
    15781 ggaagcttcg gaggtatcgt tcgtcaccag aaaacagtag ctgtggcgct cgcttcgtcc
    15841 cacgcggcca cgcagttgat gcagctgcgc taggccaaac cgttccgggt tctcgatcac
    15901 catcaccgtc gccttcggca catcgatccc cacctcgatg acggtggtcg cgaccagcac
    15961 cttcacttcc ccggaaacga aacgccccat cacggcgtct ttttcctctt ccttgagcct
    16021 cccgtggatc agacccaacg gaatcccttt gaacggacct ttgctcaaaa cttcaaacat
    16081 cttcaccgcc gccttgagat cggcggcagg ggagtcctcc gattccgctt cgtcgattat
    16141 gggaaagacg agaaaggcct gattccccga tgcgacctcc tgccggacga tctcgtagac
    16201 ctctcccttc ttcgactcgt ggatgtagac ggtgcgcacg gcttttcgac ccggcggggt
    16261 ctcatcgatg gtctcgacgt ccaaatcccc gtagacggcc agcgccagcg tgcgcgggat
    16321 tggcgtcgcc gacataatca gcgtgtcgac ctgttcccct ttcgacttca gactctccct
    16381 ctgcttcacg ccaaatcggt gctgctcatc cacgagggcg agcccgagac gagaaaagac
    16441 gacgtcttct tggatcagcg cgtgcgtccc gatcacgagc ggcgcgtggc cggaacggat
    16501 ttcctccttg accagttctt tctccttgtt tggaaggccg ctgatcaaca atcgcggctg
    16561 gatgtcgaga cgggcgaagt catcggcgat ggttcggaaa tgctgttgcg ccaggatcgc
    16621 cgtgggcgcc atgaatgcgg cctggaaccc gctttcgatg atatctaaaa ggaccatctc
    16681 cgctacgatc gtctttccgc atcccacgtc cccttgaagc aggacgttga caggcaggtt
    16741 gttctgcagt ttcgcacgta tgctttgata gacccgcttc tgggctttgg tcagtttgaa
    16801 cggcaggcct ttgaccaacc gctcggacaa cgtcccctct atgcgcttgg ccggccgggt
    16861 gtagaactcc tcgcggttcc gacgcgcgaa caggagggcg gtctgaaaat agaagaactc
    16921 gttataggac atctttctgc gacactgttg caggtgcgct tggctttttg gctggtgcag
    16981 cccgtatatc gcgtccgcca gcgggatcaa acgccttttt tcgttgatcg acgtcggtac
    17041 tggatcctct atcgcggcga gggcatacga ggtgttcagg atcaccttac gtatcgactt
    17101 cgacgggaag cccttccgcg accggtagac cggaacgatg gttcgggtcg gacggtcctc
    17161 tggctgttcc tcctcgtagt cttgcacctc gatctcccaa cccctctgac gtgtccatcg
    17221 gacattcccc gtcaccatca ggcggttcgt tttggagagc ttctctttca gaaacggttg
    17281 attgtaccaa atcagcgcaa cctctccggt gccgtcgttc agcgtgaccg tcaggatatg
    17341 caagccgtag ttcgtacgct tttccatcac gggaccgaga gtgccgaaga cagtggcctt
    17401 gatgttcggg aaaagctccg aaacccgata gacccgtcgt cgatcgtgat aggtgcgtgg
    17461 gaaaaagaaa aacaggtcct tcaacgtttg gacatcaacg gaattaaaaa aatccgtcca
    17521 cctcggcccc acgcccttaa cgtattgcac gggcctttga agcagctcct tcagtttctg
    17581 agattcagac tcagacgggg tctcgagcgg cggcggaaac aagaaaatcg ctttcagctt
    17641 gtccaacatc gcgaacccgt tggccaaccg cttttcctgc cggtcagccg gcagcgtgtc
    17701 gaatcggtca taatgggaca cgaacagcct gagtttctct ccctcttctc cggctgtttc
    17761 ccactccagc ttgtctattt gattcaatgt cgaaagcaaa cggcggtgca aggaggcttt
    17821 ggaggcttcg tcctctcggg cctttttcag gagaccttcc gattgcgaga taaactcctc
    17881 gatcagtatc acgctactcc accagccgat ggatgaccgt cgtcaccttt ccgatcatcc
    17941 ggcacagaga ggcccaccgc tccttgtcga agaagagggg cggataggcg ctgttttcgc
    18001 tcttgaggat gacgccgttc gggtcatagt aaacccgttt cacgacggct tccgtgtcgg
    18061 ggaccatcac gaccgcgatc ccgccggaag acaccggaag atcgcatttc acgaccacgt
    18121 aggactgatc gggaatcacc ggcaccatac tgtctccggt gacgagcagc aagaagtcgc
    18181 cttctactcc aggtagaacc gggtaggtac cgatctgctg ctcttcggca tagagcggtt
    18241 tacccgcaga gatcttcccc aatacaggta gcctgcgaac gagctcttcc ccggtgggta
    18301 tcgcacgctc gagccctaga agcgtttcga tcgagacgcg atagtgctcg gccagtttct
    18361 tcagagtgat cgctgaaggg acggacttcc ccgtttcgaa cttagagacc atctgcggag
    18421 agacgcccaa gacctgcccc agttccgttt ggttctccga atgctccaca cgcagcttcc
    18481 tgagcgcttc gccaaaggca gacacgcttt tcatacattt cacctcggtg acggattttg
    18541 tgagggtttc ccctctctct cctctgacat tataccataa aaggggaagc ggttaaccca
    18601 cggtttacaa cgtgtagttg acaaccgtga gttgaagtga taaaattcta cctgtagttg
    18661 cgaatccgga tcgcaccgca caacaccaaa cacaaaaaat cacgctaagg aggtcacaca
    18721 tgggattaaa cgatccagtc aatgtagatt taacgcaaaa ggtagccgtt ctgattttgg
    18781 acaccggagt tttgaaaaat gaaaagcccg tcttgaaacg cattcgcttt ccggtcttgt
    18841 cgacagccac gaaccaacag gtctatgacg ccgcctatgc cctggcggag tacacggaat
    18901 actcggtcta cgacattgag atccagaata tggactctct cgatccgatc gactaatcgt
    18961 aaaggaggaa aaggatgatc acactggaat tgaacttcaa aaacgccatc tcggggaaga
    19021 gtaaacgact gtcgattccg gagccggtgg acaacatcga cgagttgact gaggctttgg
    19081 atacgcacat ggtgacgtac gttcagccgg ttctatcggg tagtctggtg ttcgcgtccg
    19141 cccggctggt caatcgcgaa atcaccgaac tcatcgaaaa gtaattctgc aggaggtgga
    19201 agatgaaaaa ggccgcgctc aatctacccg cgattctgaa gatgatctgc accttagccg
    19261 ccttagttgt ggaagaaccc gggaaagggg cggaaaagaa acaaaaggcg atacagttgg
    19321 ttcacgaatt cattctgtct atgggtatcc acatccctaa agccgtgtta gatctcgtac
    19381 tgccgcctat gatcgatcaa gtcgtcggca tactgaacca aacggtctgg ctaacaccga
    19441 ctacgctggt tcgctgaccc aacgggggag aaatcccccg ttttttttac ccattggagt
    19501 gcgcctgggg tagcgccgac tacccagtcg gtgtaccttt ttggatcgca cttctgggag
    19561 cgccctggga acgccgaact ttgcggttaa tcgcggggta accagcgggc tgacttcgga
    19621 gcccacgaag tctcgcgatc cgctcttgtg tctttctcaa aaccaaaaga agtgtcgcac
    19681 agagggcaca gagtacacag agaaacgccc tgggaacgcc ttcggggcgc cttgggaacg
    19741 ccgaacttta gttcggcatg tttttttccc tttctgcaag cacagaagaa gccttaagcg
    19801 cttacgccgc gctgcccgcc gtgtggggtt atcgcagggt taccagcggg ctgacttcgg
    19861 aagcccacga agtctcgcga tactgatgaa gccccacgat ccgcattccc actctttatt
    19921 agtgtataat acccgtgaaa tgatttcccg tagggaggcc aaagatggag aagttttata
    19981 acccccacga tatctttttc aaagaagtgc tgggggatgt tgtaaatacc caagcgttct
    20041 tagagaccta tctcccctat gaagtcttac agactatcga tgtcggcacg atacaggcgg
    20101 aaaaggacag cttcatagaa caagacctga aggagttcta cgcggatttg ttgt
//
LOCUS       DAOPXN010000020        19437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2845_length_19437_cov_5.196419, whole genome shotgun sequence.
ACCESSION   DAOPXN010000020 DAOPXN010000000
VERSION     DAOPXN010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 19437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19437
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2845_length_19437_cov_5.196419"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..387)
                     /locus_tag="PKM49_02100"
     CDS             complement(262..387)
                     /locus_tag="PKM49_02100"
                     /inference="COORDINATES: protein motif:HMM:NF020757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81641.1"
                     /translation="MHKQMSSGVSYGEQLVFDIERLKRHFPSVPVAIILIGCDED"
     gene            complement(595..1581)
                     /locus_tag="PKM49_02105"
     CDS             complement(595..1581)
                     /locus_tag="PKM49_02105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNY81642.1"
                     /translation="MARIADHFDPNATIVLYEGDCLTLLGTIPEETVQLVVTSPPYNI
                     GKEYEEKSSLSAYYQSQKRVIEECYRVLKPEGSICWQVGNYVDNGEILPLDIFLYPIF
                     QELNLQLRNRIIWHYGHGLHATKRFSGRYETILWFTKTDDYYFNVDPIRVPQKYPEKK
                     YFKGEKRGELSCNPLGKNPTDVWDIPNVKANHIEKTIHPAQFPVELIERLVLSMTKEG
                     DWVLDPFCGSGTTQIASMIHQRKAIGAEIEKKYVEIARSRIMAAEKGELRIRPMERPI
                     YDPSAKTPSIHPKIVHTGKSNKTVPLSPIDLNRQFKNNSVIETSLRFAKSIR"
     gene            complement(1612..3111)
                     /gene="lysS"
                     /locus_tag="PKM49_02110"
     CDS             complement(1612..3111)
                     /gene="lysS"
                     /locus_tag="PKM49_02110"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNY81643.1"
                     /translation="MSDSIREQRLQEIAELRAQGVNPYPHEFERTHTTDKILETFAYL
                     QPEQTLESETVHCAGRVIAIRDHGKSAFFVISDYYGKIQVYIRQAGVGEEAYSFFKKH
                     INVGDYAGIVGFPFRSKTGELTIYTQQIQLLTKTVRPLPEKWHGIKDKEVIYRQRYVD
                     LIANDESMKRFKVRFEALKLIREFMNNRQFVEVETPMLQYILGGASARPFVTRMNAYD
                     IDLYLRIAPELYLKRLIVGGFDRVYEINRNFRNEGVSYKHSPEFTMMECYQAYADYED
                     MMELTESLMSSVVKKMFGTYLLTYQGVEINFEPPWERIPMIDFINRHLQMDVDSSSDA
                     QLLDYLKQRGQEPELTDRGHLIEALWDLVEDKVVQPVFIIDHPIEISPLAKKHRSKAG
                     LTERFELIIYGREMANAFSELNDPVDQRERFEGQVKLRESGDEEAQMMDMDFVRALEY
                     GMPPTGGLGIGIDRFIMFLSDAPSIRDVIAFPLVRPEMVYQEYDEEGEE"
     gene            complement(3128..3610)
                     /gene="greA"
                     /locus_tag="PKM49_02115"
     CDS             complement(3128..3610)
                     /gene="greA"
                     /locus_tag="PKM49_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNY81644.1"
                     /translation="MAKSKDIIYLTKEGFDSLNEELKQLREKLMYEIAQRIKEAREFG
                     DLSENSEYEEAKNEQGRINGRIIEIENILQKAEIIDPNDIQTDVINLGHQVLLKDLKT
                     NKEQKIKIVNSQEADIFEKKISVESPLGKEISGRRVGEEIRVKTPGGIQRFKIMGIEL
                     "
     gene            complement(3611..4069)
                     /gene="nrdR"
                     /locus_tag="PKM49_02120"
     CDS             complement(3611..4069)
                     /gene="nrdR"
                     /locus_tag="PKM49_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNY81645.1"
                     /translation="MKCLFCGYEDTKVADSRPAEENKAVRRRRVCPSCRARFTTYERY
                     EMQPVVVIKRDGRREPFKREKILNGLIRSCEKRSISEEQLNALVSDVELQIQRSDLTE
                     VKTTQIGEWVMEALKKLDNVAYVRFASVYKDFRDIDQFRHIIDELGRGGT"
     gene            complement(4066..5013)
                     /gene="whiA"
                     /locus_tag="PKM49_02125"
     CDS             complement(4066..5013)
                     /gene="whiA"
                     /locus_tag="PKM49_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HNY81646.1"
                     /translation="MSFCSMVKEELAAAPCGDTRSCLLELFGMLKFRGSILLSGSQED
                     KSHSYVFHISPVYVTRRVLSLLKRLGYRDIEASYELHRYFERQKSFRVTIPMDLFNQD
                     LWERFRIDSRAESWQSVLSQDTLYIGDFLRGCFLVGGYISDPKRLYHLELIEKESDVI
                     VRFVAELLRKHYRMTPGIVLSAKGYKFYIKSVIDMIALLEMMGAAVGAEKIETIADQR
                     KVKADFNRTINFTLANANKTARSNFTQIRAIRTIQKTLGLGALDAESREIALLRLNNE
                     DLSLSELDSLMKSPIGKSALYNRIKKMIKLAHLLDEEER"
     gene            complement(5027..5974)
                     /locus_tag="PKM49_02130"
     CDS             complement(5027..5974)
                     /locus_tag="PKM49_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HNY81647.1"
                     /translation="MKDIVVIGGGTGLSTLLRGLKNIRAFRLTAVVTITDEGGSSGTI
                     REEMHIPPPGDVRNNIVALAEDENLLTQLIRYRFTDTGVFENHSVGNIVLAALTKILG
                     SFPEAVMKLSDVLAIQGTVYPVSSQIVRLIAVNNLGETLIGEKGIERNRGKIIYITTN
                     KRFSAIPEVIRALKSARGIVFGPGSLYTSIIPNILADGVPEAIAANPCRKVYVANIMT
                     QMGETLGMTLSDHVSEIERYLGSPLDDLIANSEGIPETLMQRYAEQGAEPVRIDSAAI
                     PRRVSIEKLLKLVVDDRDQLLKVRHDEIKLATILKRIFS"
     gene            complement(5993..6871)
                     /gene="rapZ"
                     /locus_tag="PKM49_02135"
     CDS             complement(5993..6871)
                     /gene="rapZ"
                     /locus_tag="PKM49_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNY81648.1"
                     /translation="MHNEKTRNLVILSGLSGSGKTTCLGLLEDIGFFCVDNIPPQLLK
                     DMMSLFEHMDVQKMALVVDARWKQRLDIAKETILQYREMHPEVDIRIIFLEAQETHII
                     RRYALTRRKHPLTDGGSLAEAYRKEIDFLAPIKEIADNVIDTSNWNTHELREALKSIV
                     VWSFESRGRITALRLISFGYKHGMPLGADFVVDTRFLPNPYYRPELAHLDGLDPLLGD
                     FFSKFPIVSDFIQTVISMLEIGLHRYEEEGRDSVTVAIGCSGGRHRSVFLTERIREFF
                     SAEGFSVYVEHRDIRL"
     gene            complement(6927..7226)
                     /locus_tag="PKM49_02140"
     CDS             complement(6927..7226)
                     /locus_tag="PKM49_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81649.1"
                     /translation="MKRKITFDLGGYTFSFLSDEPGEKIQKMKTELENELSRYRQHIE
                     SNPEEGLKEVFVLMLLNHVTRETQLEEEVKRLEEKVERLSLEVGHVKSNRSDMVG"
     gene            7899..8615
                     /gene="rnc"
                     /locus_tag="PKM49_02145"
     CDS             7899..8615
                     /gene="rnc"
                     /locus_tag="PKM49_02145"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNY81650.1"
                     /translation="MVSGFERRLGLTFKDKDLLKLALSHSSWVYEQAVKTAETAPQSN
                     ERLEFLGDAIVGFLVVEKLYRDYPLENEGTLARVKAVMASEPLLSRAAKALDMGKFLF
                     LGKGEIHSGGRERDSILADAFEAVCGAIYLDLGMDPVRLLLERLLFPYLQDVFDDVIC
                     LDYKSKLQEFTQGVDRLLPEYRLVSVSGPSHQPLFVVDVYLESRLLARGEGRTKKEAE
                     QNAAEGGIKLLSKERSDGLG"
     gene            8602..9594
                     /locus_tag="PKM49_02150"
     CDS             8602..9594
                     /locus_tag="PKM49_02150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81651.1"
                     /translation="MGSAKSNGIIPVFLSYMGCPNQCLFCSQKGVTEDDSQRSFEELD
                     ERVMTFISSRSNRSNSSEVSSRRFEIAFYGGTFTSIPEPMQEAFLRWAYRWLERGSID
                     SIRVSTRPDDLTVDKLQCLARYGVKTIEIGAQSFDDHTLRLNRRSYTSEDIDRAFGLL
                     KSSPFRVGVHLMTHLYGSSEAIDAQSLSAALSYRPAFLRLHPTLVLRHTGLEGLYRER
                     RYRPASLFEGVDGCSDALLLCRSAQTPVARMGLHIPANRVSEVVAGPYHPAFGDLVSK
                     MADLKELIASERLVDYAGDGLDQKYREALGSHKGFFAKWFRERSREARHVRARR"
     gene            9612..10637
                     /locus_tag="PKM49_02155"
     CDS             9612..10637
                     /locus_tag="PKM49_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81652.1"
                     /translation="MRFFLFLMGILLFCNSAWADFKTVLTKYFETCPAAVTTELVWQN
                     ANDEYNAALLRASSKLEILMADVARYEKQLAYTSKKWALIADLIDALLDWKLAQIEKN
                     IAEVEYNMYSEDYTNKQKAFTYGGVSETEVQIALIRRDLHLAELEYYKNYRAQLETHL
                     KETLSVSEIPTITLPLAALPTLNEETQKKAKDGSIEIKIAQSSNEIEMTRYRLSSAGM
                     TTAYQADYSKRMAKIHELNIKSLEQDLYYDLARYHEGLKIATARLKASQDEKNLQLNQ
                     LPKVQEAHTKGYITANDYYKKLLEIYAYDRTAHHAEKEYLESLIAFLKQSNADPIAVF
                     PLYVQTK"
     gene            10685..11563
                     /locus_tag="PKM49_02160"
     CDS             10685..11563
                     /locus_tag="PKM49_02160"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNY81653.1"
                     /translation="MREIVLRVPRERAEEAESLILVGLSDTEGPSEFNYYLEEPEGLT
                     ESVFHLFVPEADAAVVLSWIEENRGVFGKASVRVVVDGDWSAFYIKTLEPYWLTPNVL
                     VDPFVGTEREILLVRIACGKAFGIGDHSTTRMAVELLQKYLKRGQRVLDVGYGTGILS
                     ILASKWGARSVTGFDIDPDAVREARGNARLNQCDPAPDFREGDYVHISERVPFDLFVS
                     NIYVEVLEEAVKASLNSVERTEGSEKVYILSGILETKWRIFDKKMRGLGLCFVEKRID
                     SPWVAGVWTLKPDTVR"
     gene            11591..12280
                     /locus_tag="PKM49_02165"
     CDS             11591..12280
                     /locus_tag="PKM49_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81654.1"
                     /translation="MIEKNFRPSIRRLLFALFLLGAGIIYAQQAPTLTVANTKDESWD
                     FTIRVMTQTFATETVFFSQAEGVTTFTANAVLFSQGQPAILLASGKTDDSRIVEYALD
                     VDYNGKRTMIEATVKGDVLEVRSGEKTVTLPWTPNTILLDNNMSFMWQYFLYYYPYAG
                     TNDFIAVIPQLVASQDTIAFPFTIQRAVESDGKVTLNFSLASSLGIINASNRYRLSHI
                     QIGVTKMERTR"
     gene            12280..12831
                     /locus_tag="PKM49_02170"
     CDS             12280..12831
                     /locus_tag="PKM49_02170"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HNY81655.1"
                     /translation="MAQGKITVIVGPMYSGKTSELLSYAEIYKLGKKRYQIFKPIIDN
                     RYRLQEIRSHSGMKESAIPIAHSADAERYITDEKAIFFDEVQFFDRDLADVVKKIRHL
                     GKDVICAGLDMSFKENPFETTALLLCLADVVIKKRAVCHECGEYNGIITYKYVDNESE
                     IDVGGFDKYFAVCLDCYHKLTQQ"
     gene            12851..15223
                     /gene="priA"
                     /locus_tag="PKM49_02175"
     CDS             12851..15223
                     /gene="priA"
                     /locus_tag="PKM49_02175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNY81656.1"
                     /translation="MTTYYEVAISGNPLRRAFTYASSEEIDPGTRVVVDFRNRVTIGY
                     VLDVAHPSEELAAKTKALLEIVDYYPLLTSTHIELAKRTARYFFSPIGKVCDLFFSAS
                     PQIVVQTRLVRSSYDEMPGFESAEASVPAEVFFQRVGRKKGRVLLEEWLKEKRVVRAI
                     GLKTRTQRTRTDSWIRPVDDLGQALSKLEKTSGKEWAIMQHLLLHAEEPASYVKKTFR
                     VNDATLLSMAKRRLLLIEEMDDSEATYVEERGRVNLTDRQKEVSRSILRDIDRSAPVL
                     EHVIHGVTGSGKTEVYFSVIAEILKRGRAVLFLIPEISLSPQTIARVRTRFPDVEVGI
                     YHSALKPAQRQREWQKAVIGETKIFLGTRSALWVPMPNLGLIIVDEEHDTSYAQEDAQ
                     PYYDTKRVVRWLSELGGIPVVYGSATPRIETFYEAQELRQTRLHTLPERPLGISLPHV
                     RLIDMIHAERVNAFFSKELVQSMDEVLSRGKQVFLLANRKGFAPFLICSMCGFVAKCV
                     QCDVSLTYHQATRRLKCHYCGFETSLYTRCPSCEREGLHFSGYGTEKVEEVLKTLFPD
                     RRVLRMDKEAIDSLEKLSAALERIKDKKIDIVVGTKMISKGLDFPEVELVGVLDADHF
                     LYFPDFRSSERTFQLLSQMSGRAGRAFSESLVLVQTRSIDKTSIQKSALHDYPGFFAQ
                     ETQNRKMASYPPYGHIVILNFTCYDKEVGAALSSKISTELKESLGPENKMLGPTEALI
                     FKLSGQYRYRTMVKTQQPEKVVGAIESMFDRNPDYEKTVSVVVDPLGMIL"
     gene            15237..16382
                     /locus_tag="PKM49_02180"
     CDS             15237..16382
                     /locus_tag="PKM49_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY81657.1"
                     /translation="MNEFLLSLWWNFFKLIGVPFFTLRFRTRYEGPSPKTQFIVVANH
                     TRDIDPALISWFLKRPAHWLSAQEAFINPFRRALLTSLGAFQIIRYETDLLVVKRIIE
                     AVKAGKCIAMFPYGNASWDGVFEAPGQGTLDLVRFLKIPVLAIRAEGAYLSFPRWARR
                     RRKGPIFLKAALLDPDEAIRHITFSEWDWQRERMERYPGKDKAAGLERILWFCPFCGG
                     FRSIAIEGDSAKCRACRSVYTVDDYGFVEGNTIAELLTQQLAFLSDYLRIQEKVSLGR
                     VDLFIRNKGALRYHSRYRATLSLFPDSISIEPENGGRVRTQEGAAALNTCFYYENIKH
                     QVGFQKTMLEFGYDEEVVRIRTDDSSLLLLKAIEQKKREATHVLDYD"
     gene            16363..17703
                     /locus_tag="PKM49_02185"
     CDS             16363..17703
                     /locus_tag="PKM49_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81658.1"
                     /translation="MYWTMIDDIVYILMFFAGAMLLKRFVPFLRKAIIPNSILAGFIG
                     FILGPNVLNWVHFDVETLGQMIYHLTAIGFIALSLRKVTRGKNTVQYVNAGMIIVATY
                     LFQGIFGFLLFWGWHALEPQVTPIIGFLVPMGYGQGPGQAFSIGTQWEGLGLANGGAL
                     GLAIAAAGFAWATLGGIFILNVVLKRKKLRDSVQFIGKKPIQVQDFEFSDIDGLTVQL
                     VMIVSVYMIVYGALFLLTRYVLDFFPIGKTLGPVLWGFHFAFGAAAAVFFRKGYEVLH
                     EKKIVHENYLNDFLLHRIAGGVFDFMVAASISAIALRKITDVLLALFVICFIAGVLTY
                     FFVAWLVRKTMKEKQLENILALFGNLTGTISTGMALLREVDPGLESGASDNLVFGSGV
                     AIFIGIPMLLVLSLPPFVLSTGNQAYYWYAFLLMVGFFLGMFLLWFRPRFKRNP"
     gene            17746..18495
                     /gene="gpmA"
                     /locus_tag="PKM49_02190"
     CDS             17746..18495
                     /gene="gpmA"
                     /locus_tag="PKM49_02190"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HNY81659.1"
                     /translation="MYTIVLLRHGESVWNKENLYTGWTDVGLSEKGVEEARSAGRVLK
                     QLGYEFNMAFTSVLKRAILTLELALTEMDQLWIPVEKSWRLNERHYGALQGLNKAQTA
                     QEYGEQQVKLWRRSYDVPPPVLTPDDARYPGNDPRYQDLDPRVLPLSECLKDTLERVL
                     PYWHDRIAVEIKKNRKILIAAHGNSLRALVKYLDRIPDDEIVELNIPTGVPLVYELKK
                     DLTPIRHYYLGDEETIRKAQEAVKNQGKAKG"
     gene            19348..19422
                     /locus_tag="PKM49_02195"
     tRNA            19348..19422
                     /locus_tag="PKM49_02195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19380..19382,aa:Val,seq:gac)
BASE COUNT         4448 a         4967 c         5194 g         4828 t
ORIGIN      
        1 aaaaaagaag gtttcgcacg gagagcaccg agggcacgga ggagtgatga aagcgttgaa
       61 gacatgccgg actgaagtcc ggagttccca ggggttccct gaaggcacag aggccccaga
      121 gagcacactg ggaacgccaa cttttaagtt ggcaggtctt tagcgggccg taaaaagggc
      181 gaagagcgta tcgcggggtc ccgttggtta ccccgcgatt aacctcaaag tgcggcgttc
      241 ccaggcgctc ccaaaaatcg gttagtcttc atcgcagcca atcaaaatga tggctacagg
      301 gactgaaggg aagtgccttt ttagtctttc tatatcaaat acgagttgtt ctccgtacga
      361 aacgccgctt gacatctgtt tgtgcatact ataacaagga acgatttcta tgccgacgtc
      421 catttgtccg ctgttataaa aaactggaat ttcgccaagt cataaaacat gaaggcgtat
      481 ttccctaatt gtacttcaat gagcactctc tttttgcaaa aacgatttgt ttgtaagctc
      541 ctggtacttc gatctcatac ccttgtcggg tttgatcctg atttgggtcg attactatcg
      601 tatacttttc gcgaacctca gcgaagtctc gatcaccgaa ttatttttga actgtcggtt
      661 tagatcgatg gggcttagag gaacagtctt gttcgatttt cccgtatgca ctatcttggg
      721 atgaatactc ggcgttttcg cgcttgggtc atagatcgga cgctccatag gtcggattct
      781 tagttctcct ttttctgcgg ccatgattct gcttcgtgcg atttcaacgt actttttttc
      841 tatttccgcc ccgattgcct ttcgttgatg aatcattgaa gcgatttgcg tggttcctga
      901 cccacagaac ggatcgagta cccaatcgcc ttcctttgtc atcgagagta caaggcgttc
      961 gatgagttct accgggaatt gcgctgggtg aatggttttc tcgatatgat tcgcttttac
     1021 gtttgggata tcccaaacat ccgttggatt tttccccaaa ggattgcaag agagctctcc
     1081 ccgcttctca cctttgaaat acttcttttc tgggtacttc tgcgggacgc gtatgggatc
     1141 gacgttgaaa taataatcat cggtttttgt aaaccataat attgtttcgt atctgccaga
     1201 gaacctcttc gtcgcgtgta aaccgtgacc ataatgccat attatccggt tcctgagctg
     1261 aagattcaat tcttgaaaaa ttgggtatag gaatatgtcg agaggcaaga tttctccgtt
     1321 gtcaacgtag tttcctactt gccaacagat actaccctca ggcttaagca cccggtagca
     1381 ttcttcgatt actctctttt gtgactggta gtaggcgctt aaggaggatt tttcttcgta
     1441 ttcttttcct atgttatagg gaggtgaggt gaccacgagc tggaccgttt cttctgggat
     1501 ggtgcccaac agagttagac agtcgccttc ataaagaact atcgttgcgt ttggatcgaa
     1561 gtgatcggct atgcgcgcca tctgacctcc tttcacccac aattactctc cctattcctc
     1621 tccttcctcg tcgtattcct gataaaccat ctcgggccgg acgagtggga aggcgatgac
     1681 gtctcggatg ctcggcgcgt cagacaggaa catgatgaag cggtctatcc cgatgcctag
     1741 gccgccggtt gggggcattc cgtattccag cgctcgtacg aagtccatat ccatcatttg
     1801 cgcttcttcg tctccgcttt cacgcaattt cacctgtcct tcgaaccgtt cgcgctggtc
     1861 cacgggatcg ttcaattcgc tgaacgcgtt cgccatctcc cgcccgtaga tgatgagctc
     1921 gaatcgttcg gtgagaccgg ctttggaccg atgcttttta gccaacgggg agatctcgat
     1981 cggatggtcg atgatgaaca cgggttgaac gactttgtct tcgacgaggt cccataacgc
     2041 ttcgatcaga tgcccgcggt ccgtcagctc cggctcctgg cccctctgct tcaggtaatc
     2101 gagtagttgc gcatcgctgc tggagtctac gtccatctgg aggtgccggt ttatgaagtc
     2161 tatcatcggg atgcgctccc acgggggttc gaagttgatc tccacgcctt gataggtgag
     2221 gagataggtg ccaaacatct tcttgactac cgatgacatc aggctttcgg tgagttccat
     2281 catatcttca tagtcggcgt aggcctgata acattccatc atcgtgaatt ccgggctgtg
     2341 cttgtagctg accccctcgt ttcggaagtt gcggttgatc tcgtagactc ggtcgaaacc
     2401 cccgacgatc aagcgtttta agtagagttc gggcgctatt cttaaataca gatcaatatc
     2461 gtaagcgttc atacgggtca cgaacggcct cgcggaagcg ccacctaaaa tgtactggag
     2521 catcggcgtc tctacctcga cgaactgccg gttgttcata aactcgcgta tgagcttcaa
     2581 cgcctcgaaa cgcactttga accgtttcat gctttcgtcg ttcgcaatca agtcgacgta
     2641 ccgttgtcga tagatgacct ctttgtcctt tattccgtgc catttttcgg gtagcgggcg
     2701 tacggttttc gtcaacaatt ggatctgttg ggtgtaaatg gtcaactccc cggtctttga
     2761 gcgaaaaggg aacccgacga tgcccgcgta atcaccgaca ttgatgtgct tcttgaagaa
     2821 ggagtacgct tcctctccca ctccggcttg gcgaatgtaa acctgtatct tgccgtagta
     2881 gtcggatatc acaaagaagg ccgatttgcc gtgatcgcgt atggcgatga cccggcccgc
     2941 acagtgtacc gtttcgctct ctagggtctg ctccggttgg aggtaggcga aagtctccag
     3001 tattttgtca gtcgtatggg ttctttcgaa ttcatgcgga taggggttaa ccccctgagc
     3061 gcgtagttcg gcgatctctt gaagccgttg ctctctgatg ctgtctgaca tacttagcct
     3121 cctgtgttta gagttcgatc cccattattt taaaacgctg gatacctccg ggagttttca
     3181 cacggatctc ttctccgacc cttctgccgc tgatttcttt tcctaacgga gactcgacgc
     3241 tgatcttttt ttcgaagatg tccgcttctt gtgagttcac gatcttgatc ttctgctctt
     3301 tgttggtttt taggtccttg agcaacacct ggtgtcccaa gttgatgaca tcggtttgta
     3361 tgtcattggg gtcgatgatc tccgccttct gcaagatgtt ctctatctcg atgatccggc
     3421 cgttgatccg cccttgctca tttttcgcct cttcgtattc gctgttttcg ctgaggtccc
     3481 cgaactcccg ggcctctttt atccgttggg cgatttcata catcaatttt tcccgaagct
     3541 gcttcaattc ctcgtttaac gagtcgaatc cctctttcgt caagtagatg atgtctttcg
     3601 atttggccac ttacgttcct ccccttccca gttcatcgat gatatgtcga aactgatcga
     3661 tatctcggaa atctttgtag accgacgcga accggacgta agccacgttg tctagcttct
     3721 tcaaggcttc catcacccat tcgccgatct gcgtcgtttt aacctccgtc aggtcgcttc
     3781 gttggatttg cagttctaca tccgatacca aggcgttgag ctgctcttcc gagatgctcc
     3841 gtttttcaca cgagcggatc aatccgttca ggatcttttc gcgtttgaaa ggctcccgcc
     3901 ggccatctcg tttgatcacc acgaccggct gcatctcata ccgctcatag gtcgtgaatc
     3961 tggcacgaca agacggacag acgcgccgtc tgcgaacggc tttgttctct tcggcgggtc
     4021 tggagtcggc gactttcgtg tcttcataac cgcagaaaag gcatttcatc tttcttcctc
     4081 atctaataga tgagccaatt ttatcatttt tttaatgcga ttgtacaggg ccgacttgcc
     4141 tattggactt ttcatcaagg aatctagttc gctcaagctc aggtcttcgt tgttgaggcg
     4201 cagcagggcg atctcgcggc tctcggcgtc caaggcccct aaccccagcg tcttttgaat
     4261 cgtgcggatc gctcggatct gggtaaagtt gctgcgcgcc gttttattgg cgttcgcgag
     4321 cgtaaaattg atcgtccggt tgaagtccgc tttcaccttc ctctggtccg cgatcgtttc
     4381 gatcttttcg gcgccaacgg ccgcgcccat catttccagc aacgcgatca tatcgatgac
     4441 gcttttgata tagaacttgt accctttcgc ggagaggacg atgccggggg tcatacggta
     4501 gtgttttctc aacaattcgg cgacgaagcg tacgatgaca tcgctttctt tctcgattaa
     4561 ctcgagatga tacagtcgtt tcgggtcgct gatgtacccg ccgacaagaa aacatcctct
     4621 caggaaatca ccgatgtaca gcgtgtcttg actcaggacc gactgccagc tctccgctcg
     4681 agaatcgata cggaaacgct cccagaggtc ttggttgaaa aggtccatag ggatcgtcac
     4741 cctgaaagac ttttgccgtt cgaagtagcg gtggagttcg taggaggcct cgatatctcg
     4801 atagcccaat cgtttcaata gggacaggac gcggcgggtg acgtagacgg gggagatatg
     4861 aaagacgtag gaatggcttt tgtcttcctg gctacccgaa agcagaatgc ttccgcgaaa
     4921 tttgagcata ccgaagagct ccaacaggca agaacgcgta tccccacagg gcgctgccgc
     4981 caattcctct ttgaccatgg aacaaaaact caaccgcgcg cctctgtcac gaaaagatgc
     5041 gcttcaagat cgtggccagt ttgatctcgt cgtgtcgcac cttgagtagt tggtcgcgat
     5101 cgtctaccac aagcttcagg agtttttcta tagagacccg cctaggaatc gcggcggagt
     5161 cgatccgcac cggttccgcg ccttgttccg catagcgctg catcagcgtc tccgggatcc
     5221 cttccgaatt cgcgataagg tcatccaagg gagaacccag atagcgctcg atctctgaaa
     5281 cgtggtcgga aagggtcatt ccgagcgttt cacccatttg cgtcatgatg ttggcgacgt
     5341 agacttttcg acaggggttc gcggcgatcg cttctggtac gccatcggcc aggatattcg
     5401 ggatgatgct ggtgtatagg cttccgggtc cgaagactat gccgcgggcg cttttcaagg
     5461 ccctaatgac ctcaggtatc gcagaaaagc gtttgttggt cgtgatgtag atgatctttc
     5521 cgcggtttcg ttcgattccc ttctctccga ttaaggtttc gcccaggttg ttcaccgcga
     5581 tcagccgaac gatctgagaa ctcacggggt agacggttcc ttggatcgcg aggacgtcgg
     5641 agagcttcat caccgcttcg ggaaagctcc ctagtatttt cgtcagcgcc gcgagcacaa
     5701 tgttaccgac cgaatggttt tcgaaaaccc cggtgtcggt gaatcggtag cggatcaatt
     5761 gggtcagcag gttctcgtct tcggccaacg cgacgatatt gttacgcaca tctccggggg
     5821 gcggtatgtg catctcctca cgtatggtgc ccgagctccc gccttcgtcc gtgatcgtca
     5881 ccactgcggt caaccggaaa gcccggatgt tcttcaatcc tctcagaagg gtagagagac
     5941 ccgttccgcc gcctatgacg acgatgtctt tcatcgcgcg cacctgctcc gcttacaacc
     6001 ggatatcccg gtgctcgacg taaacggaaa atccctctgc cgaaaagaac tcgcgtattc
     6061 tttcggtaag gaataccgat cggtgtctcc ctccggaaca gccgatagcc acggtgacgc
     6121 tgtctcttcc ctcttcttca tagcgatgca agccgatttc cagcattgag atcacggttt
     6181 ggatgaagtc ggacacgatt gggaatttcg agaaaaaatc tcctaacagc gggtccaaac
     6241 cgtctagatg cgccagttcc gggcgatagt agggatttgg caggaaacgg gtatccacca
     6301 cgaaatcggc tccgaggggc atcccgtgct tgtatccgaa gctgattagg cgcagggcgg
     6361 tgattcggcc tcgagattcg aaggaccaaa cgacgatgga cttgagggct tcgcggagtt
     6421 cgtgggtgtt ccagttggag gtgtcgatca cgttgtccgc gatctctttg atcggggcga
     6481 ggaaatcgat ctctttacgg tacgcctccg caagactgcc cccatcggtc aacgggtgct
     6541 tacgacgggt caacgcgtac cgtctaataa tatgcgtttc ttgagcctcg aggaaaatga
     6601 tcctgatgtc aacctcgggg tgcatttcgc ggtattgcag gatggtttct ttggcgatat
     6661 cgaggcgttg tttccagcgg gcgtcgacca cgagcgccat cttctggacg tccatatgtt
     6721 caaagagaga catcatgtct ttcaagagtt gaggggggat attgtccacg caaaaaaaac
     6781 cgatatcttc caacaacccg aggcaggtcg tttttccaga accggatagc ccagaaagga
     6841 tcaccagatt cctggttttc tcgttatgca tggggcgctt ctttctcaaa aaaacagtga
     6901 agtttctgtg ttgccaaagc gttcctttaa ccgaccatat cgctacggtt cgattttacg
     6961 tggcctactt ccagagagag cctttcgacc ttttcttcca atcgcttgac ctcttcttcc
     7021 agctgcgttt ctcgcgttac gtggttcagt agcatcaata cgaaaacctc tttcagcccc
     7081 tcttccggat tcgattcgat atgctgtcgg taacgggaaa gctcgttttc gagctccgtt
     7141 ttcatcttct gaatcttttc tcccggttcg tcagacaaaa acgaaaaagt gtaaccccct
     7201 agatcaaacg tgatttttct tttcaaaaaa ccaacccccc tctatcgcct ctttatttta
     7261 ccataactac cgcgccatcg caatatccgt gtgagccaag ttatggagtg gcataactaa
     7321 gaggttcatc gcggggtaag tggtttttac gaccccgcga tccgccttat gccctctcaa
     7381 aaacgtatcg cgccgtcgcg aaaataagaa gcgcttacgc cgcgatgact atgtgggcta
     7441 aaaatcgcgc gatcgattct ggccttgttt cttgattaca ccccatagct tgaaccacac
     7501 caatatcccc gtcactggga acgccaattt taaagctggc atgccttgaa aaaaggtttc
     7561 gcacagagag cacagagaac acagagggca cagagaaaga tgaaagaaag aagacatgcc
     7621 gcactgaagt gcggcgttcc caggcgttcc cataaaaacg aaagcgccaa aaaagaaggc
     7681 atgccaaact gaagtttggc gttccaaggc gctcatagaa aagcaaaaac accaaaaata
     7741 caagaagaca tgccgcactg aagcgcggcg ttcccaggcg ttcccaggcg ttcccagtgc
     7801 gctttcggca aacgccgcgg aaggttttgc gttttttcgt gctataattt gtaatcatgg
     7861 gaaagaactg tcatatacac aaacagcgtg aagaggacat ggttagcggg tttgagcggc
     7921 gtttgggctt gaccttcaaa gacaaagacc tgctcaaact ggcgttgtct cactcttctt
     7981 gggtctacga acaagcggtt aaaactgcgg agactgcccc gcaatcgaac gagcggctcg
     8041 aattcctcgg agacgccatc gtgggcttcc tagtcgttga aaaactgtac agagattatc
     8101 ctctggaaaa cgaggggact ttggccaggg tgaaagcggt gatggcgagc gaaccgctgc
     8161 tctctcgggc agcgaaagcg ttggatatgg ggaagttttt gtttttgggg aagggggaga
     8221 tccactccgg cgggagagag agggactcga tattggccga cgccttcgaa gccgtttgtg
     8281 gcgctatcta tctcgatttg ggaatggatc ctgttcggct ccttctagaa cgcttgctgt
     8341 tcccctattt gcaagacgtt ttcgacgacg tcatctgctt ggactataag tcgaagcttc
     8401 aggagttcac acagggggtg gaccgtttac ttcccgagta ccggttggtc tctgtatccg
     8461 gaccttctca ccaaccgctc ttcgtcgtcg atgtgtacct ggagtccaga ctgctcgcga
     8521 gaggcgaagg gagaaccaaa aaagaagcgg aacagaacgc ggcggaaggc gggatcaagc
     8581 tgctttcaaa ggagagatcc gatgggctcg gctaaaagta acggcatcat cccggttttc
     8641 ctctcctata tgggatgccc aaaccaatgt ttgttttgct ctcaaaaagg ggtcaccgaa
     8701 gacgattcgc agaggtcctt cgaggaactc gatgaacgcg tgatgacgtt tatttcctcc
     8761 cgctctaacc gttcgaactc atccgaagtc tcttccaggc gttttgagat cgccttctac
     8821 ggcggtacct tcacgtctat tccggaaccc atgcaggagg cttttctgcg ttgggcctac
     8881 cgatggctgg agaggggctc gatcgattcg atacgggtct cgacccgccc ggatgacctg
     8941 accgtagaca aactgcaatg cttggcgcgt tatggcgtga aaaccatcga aattggggcg
     9001 cagagttttg acgatcacac cctgcgtctc aatcgccgtt cctacacctc cgaggatatc
     9061 gaccgcgcct tcgggcttct caagtcgtcg ccttttcgtg tcggcgtaca tttgatgacc
     9121 catctgtacg gttcgtccga ggctatcgat gcccagtcgt tgtccgctgc cctgtcgtac
     9181 cggccggctt ttttacgctt gcatccgacc ttggtgcttc ggcataccgg actggaagga
     9241 ctctatcgcg agagacgcta ccgaccggcc tccctcttcg aaggggtgga cggctgctct
     9301 gacgcgctat tgctctgtcg gagcgcgcaa acccctgtcg cgcgaatggg cttgcacatc
     9361 ccagcgaacc gcgtttcaga ggtcgtcgcc ggaccctacc accccgcgtt cggggacctc
     9421 gtatccaaaa tggccgactt gaaagaactc atcgcttccg aacgtctcgt tgactatgcg
     9481 ggggatggat tggatcagaa gtatcgggag gccctcggtt cacataaagg gttctttgcc
     9541 aagtggtttc gagaacgaag tcgcgaagcg cgccacgtta gagcgcgacg ttaagggggg
     9601 tgcggaaggt tatgcggttt ttccttttcc taatgggtat cctacttttc tgtaatagcg
     9661 cgtgggcgga cttcaagacc gtcctgacga agtacttcga aacctgtccg gccgcggtca
     9721 ccacggaatt ggtctggcaa aacgcgaacg acgagtacaa cgccgccttg ctcagagcct
     9781 cctccaagct cgaaatcctt atggcggacg tggcgcgtta tgagaaacag ctcgcctata
     9841 cctccaaaaa atgggccctg atcgccgatt tgatcgacgc ccttttggac tggaaactcg
     9901 cgcagatcga gaagaatatc gccgaagtcg aatacaatat gtacagcgag gactatacga
     9961 acaaacaaaa agccttcacc tacggaggcg tgtccgaaac ggaagtacag atcgcgctga
    10021 ttcgtcggga cctgcatctg gccgaactcg aatattacaa aaactatagg gcccaactgg
    10081 agacccattt gaaggaaacc ctttccgtta gcgagatccc gacgattacc cttcctttgg
    10141 ccgctttgcc gacgctcaac gaggagaccc aaaagaaagc caaggacggt tcgatcgaga
    10201 tcaagattgc ccaatccagc aacgagatcg aaatgacccg ataccggctt tcctccgccg
    10261 ggatgacgac cgcctatcaa gccgactact ccaagcgtat ggcgaaaata cacgagttga
    10321 acatcaaatc gctggaacaa gacctgtact atgacctggc gcgctatcac gaaggactca
    10381 agatcgcgac cgcgcgtcta aaggcttctc aagacgaaaa gaacctccag ctcaaccaat
    10441 tgccgaaggt gcaagaggcg cacaccaagg gatatatcac cgcgaacgat tactacaaga
    10501 agctgttgga gatctatgcc tacgaccgga cggcgcacca cgcagaaaag gagtacctcg
    10561 aatcgttgat cgcttttcta aaacagagta acgcggatcc aatcgcggtg tttccgctgt
    10621 acgtccaaac gaaatgaccg atcccgcgag cggaatagag acgggatccg ggaaggagat
    10681 gccaatgcgg gagatcgttc ttcgagttcc tcgagaaagg gccgaagagg ctgaaagcct
    10741 gatactcgtc ggtctgagcg acacggaagg tccgtcggag ttcaattact acctggaaga
    10801 acccgagggc ctaacagaat ctgtttttca cctgtttgtg cctgaagcgg atgcggccgt
    10861 agtattgtct tggatagaag agaatcgggg cgtctttggg aaagcttccg ttcgggtggt
    10921 tgtggacggt gattggtccg ccttttacat aaaaaccctg gaaccgtatt ggctcacgcc
    10981 aaacgttttg gtggaccctt tcgtcggcac ggaacgcgag atactgcttg tcaggattgc
    11041 gtgcggtaaa gcctttggaa ttggtgatca ctcgacgacg cgtatggcgg tcgaactcct
    11101 ccagaaatac ctgaaacgag ggcagcgggt tttggacgtg ggatacggca caggcatcct
    11161 gtctattttg gcctcgaaat ggggggcccg ttcggtgacc ggttttgaca tcgatcccga
    11221 tgccgtccga gaagcccgag gtaacgccag actgaaccaa tgcgatccag cacctgactt
    11281 tcgggaagga gactacgtcc atatatcgga acgggttcca tttgaccttt tcgtatcgaa
    11341 tatctacgtt gaggtgttgg aagaggcggt gaaggcctct ctaaatagcg tggagcgcac
    11401 agagggatcg gaaaaggtgt acattttatc cggtatctta gagacgaaat ggcggatatt
    11461 tgacaagaag atgcgggggt tgggcttatg tttcgtagaa aagcgaatcg atagcccttg
    11521 ggtcgccggc gtgtggacac tgaagcccga taccgtccgt tagccatatt agtgaaaaag
    11581 gagagtccga atgatcgaaa aaaactttag accatctatc cggcggctgt tattcgcgct
    11641 gttcctgctc ggggccggca ttatctatgc gcagcaggcg ccaacgctaa ccgtagccaa
    11701 caccaaagat gagagctggg attttacgat acgcgtgatg acccagacct tcgcgacgga
    11761 aacggtgttt tttagtcaag ccgagggcgt cacgaccttt accgccaatg ccgtgctctt
    11821 ttctcaaggt caaccggcca tactgcttgc gagcgggaag accgacgata gccgtatagt
    11881 cgaatacgcc ctcgatgtgg actacaacgg aaaacgcacg atgatcgaag caacggtgaa
    11941 aggagacgtg ttggaggtcc ggtcggggga gaagaccgtc acccttccct ggacgcccaa
    12001 caccatcttg ttggacaaca atatgtcatt catgtggcaa tactttctgt actattatcc
    12061 gtacgcgggg acgaacgatt ttatcgcggt gataccgcaa ctcgtcgcct cgcaggacac
    12121 tatcgccttt ccgtttacga tacagagagc ggtcgaatcg gacggaaagg tgaccttgaa
    12181 tttttcgctg gcgagctccc tcgggatcat taacgcgagt aatcgatacc gcctctccca
    12241 tatccaaatc ggcgtgacta agatggaaag gactcgttga tggcccaagg gaagatcacc
    12301 gtgattgtcg gtccgatgta ttcgggcaaa acctcggaac tgctatccta tgccgaaatc
    12361 tataagctcg gtaaaaaacg gtatcagata ttcaagccca tcatcgacaa ccggtatcgg
    12421 ttgcaggaga tccgttcgca ttcgggtatg aaagaaagcg ccattccgat cgcccacagt
    12481 gccgatgcgg agaggtatat cacggatgag aaggcgatct ttttcgatga ggtccaattc
    12541 ttcgatcggg acttggccga tgtcgtgaaa aagattcgac atcttgggaa agacgtcatc
    12601 tgcgccggac tcgatatgag cttcaaagaa aacccattcg agacgactgc tttgctgctt
    12661 tgtttggccg atgtggtaat caaaaaacgg gcagtttgcc acgaatgcgg agaatacaac
    12721 gggatcatta cctacaagta cgtcgacaac gagtctgaga tagacgtggg cgggttcgat
    12781 aaatacttcg cggtttgtct ggattgctat cacaaactga ctcaacaata actggggtct
    12841 tcaggccgct atgacgacgt actatgaggt agcgatatcc gggaatcccc tacggcgagc
    12901 cttcacttac gcttcttccg aagaaatcga tcccggaacg cgggtggtcg tcgatttccg
    12961 gaatcgggtc acgatcgggt acgttttgga cgtcgcccat ccctcggaag agctggccgc
    13021 caaaacgaaa gcgctcctcg agatcgtcga ctactacccc ctgctgacgt cgacccacat
    13081 cgagctcgcg aaacggaccg ctcggtattt tttctctccg atcgggaaag tctgcgatct
    13141 cttcttttcc gcctcgcccc aaatcgtagt tcagacgcgt ctcgttcggt cctcttacga
    13201 tgagatgccg ggattcgaga gtgccgaggc ctccgtaccc gcggaggtct ttttccagag
    13261 ggtagggcgg aaaaaggggc gcgtccttct ggaagaatgg ctgaaagaga aacgcgttgt
    13321 gagggcgatc ggtctgaaaa ccagaacgca gcgaacccgc accgactctt ggatacggcc
    13381 ggtcgatgac ctcgggcaag cgctgtctaa actagagaaa acctcgggta aagagtgggc
    13441 catcatgcag cacctgctct tgcacgctga ggaaccggcc tcttacgtaa aaaagacctt
    13501 tcgcgtcaac gacgctaccc ttctatctat ggccaagcgc cgccttctat tgatcgaaga
    13561 gatggacgat tcggaggcga cctatgtcga agagcggggg cgggtgaacc tgacggaccg
    13621 gcaaaaagag gtcagtcggt cgattctgcg cgatatcgac aggtccgcgc cggtgttgga
    13681 gcacgtgatc cacggggtga cgggcagcgg gaagacggag gtttactttt ctgtgatcgc
    13741 cgagatactc aaaagggggc gagcggttct ttttctgatc cctgagatat ctctttctcc
    13801 acaaacgatc gcccgggtca gaacccgttt tccagatgtg gaggtgggca tttaccactc
    13861 ggcgttgaaa ccggcccagc gacagcgaga gtggcaaaag gccgtcatag gcgaaacgaa
    13921 gatttttttg ggcacacgga gcgcgctttg ggtcccgatg ccgaatttgg gactcatcat
    13981 cgtggatgaa gaacacgaca cgagttatgc gcaggaagac gcccaaccct actatgacac
    14041 gaaacgggtc gttcgatggc tcagcgagct gggtggcata ccggtcgtct acgggtccgc
    14101 caccccgcgg atcgagacat tttacgaggc tcaagaacta cggcaaacgc ggttacatac
    14161 attacccgaa cgccccctcg gtatttccct tccgcacgtc cgtttgatcg atatgataca
    14221 cgccgagaga gtgaacgcgt tcttttcaaa agagctcgtg cagagtatgg atgaagtttt
    14281 gtcccgcggg aaacaggttt ttttattggc aaaccgaaaa gggttcgcgc cctttttgat
    14341 ctgctcgatg tgcggtttcg tcgccaaatg cgtgcaatgc gacgtctcct tgacctacca
    14401 tcaagcgacc cgtcggttaa aatgccacta ttgcggattc gagacctcgc tttatacgcg
    14461 ttgcccctct tgcgaaagag aagggctcca tttctccggt tacgggacag aaaaagtgga
    14521 ggaggtgttg aaaaccctct ttccggatcg acgtgttctt cgaatggaca aagaggctat
    14581 agacagcctc gaaaagttga gcgcggcctt ggaacgcatt aaggacaaaa aaatagacat
    14641 cgtggtgggc acgaagatga tctcgaaagg actggacttc ccagaggtgg agttggtggg
    14701 tgtattggac gccgatcatt ttctgtactt ccccgatttc cgttcttccg agaggacctt
    14761 ccagctgttg agccagatgt cgggaagagc cggtcgagcg ttctcagagt ctttagtgct
    14821 cgttcagacg cgctcgatcg ataaaacttc gatccagaaa agcgccctgc atgattatcc
    14881 gggattcttc gctcaagaaa cccaaaaccg aaaaatggca agctatcctc cttatgggca
    14941 tatcgtgatt ttgaatttta cgtgttatga taaggaggtc ggcgccgcgc tttcatcgaa
    15001 gatcagtacg gaattgaaag aatcgctcgg tcccgaaaac aagatgttgg gaccgacgga
    15061 agccttgatc tttaagctct ccgggcaata ccgctatcga acgatggtca agacacagca
    15121 accggagaag gtagtcgggg ctatcgagag tatgttcgac cgaaacccag actatgaaaa
    15181 gacggtcagt gtcgtagtcg accccctggg tatgatactc tagcgggagc ggaatgatga
    15241 atgaatttct tttgagtctg tggtggaact tctttaagct catcggtgtg cccttcttca
    15301 cgttgagatt ccgcacacga tacgagggcc cttctcccaa gacacagttt attgtggtcg
    15361 cgaaccatac gcgagatatc gatcccgcct tgatctcttg gttcctcaag cgtcccgcgc
    15421 attggctctc cgcgcaggag gcttttatca acccctttag gcgcgccttg ctcacttcgc
    15481 tcggcgcttt tcagatcata cggtacgaga ccgacctctt ggtcgtgaaa cggatcatcg
    15541 aagcggttaa ggctggaaag tgcatagcga tgttccctta cggtaacgct tcttgggacg
    15601 gggtttttga agcgccgggg cagggaacgc tggaccttgt gcgttttttg aagattcccg
    15661 tcctggctat acgcgccgag ggagcctact tgtctttccc gcggtgggcg aggagaaggc
    15721 gaaagggtcc gattttctta aaagccgctt tactcgaccc ggatgaggcg attcggcata
    15781 tcactttttc cgaatgggat tggcagaggg agcggatgga gagatacccc ggaaaagaca
    15841 aggcggcggg tttagaacgg attctgtggt tttgcccgtt ttgtgggggt tttcgctcca
    15901 tagccattga gggtgacagc gcgaagtgtc gggcgtgtcg atcggtctac accgtggacg
    15961 attacggatt cgtggaggga aacaccatcg cggagttgtt gacccaacag cttgcgtttc
    16021 tttctgacta cctgcgcata caagagaagg tgagccttgg gcgcgtagac ctgtttatca
    16081 ggaataaagg ggcgctccgt tatcacagcc gctaccgggc cactctctct ttattcccag
    16141 actcgatatc gatcgaaccg gaaaacggag ggcgggttcg gacgcaggaa ggtgcggcgg
    16201 ctctaaacac gtgcttctac tatgagaata tcaagcatca ggttggtttt cagaaaacga
    16261 tgctggaatt tggctacgac gaggaggtcg ttcgcatccg aacggatgac tccagcctat
    16321 tgctcctcaa ggcgatcgag caaaaaaaaa gggaggcaac tcatgtactg gactatgatt
    16381 gacgatatcg tctatatctt gatgtttttt gcgggggcta tgctgctcaa gcggttcgtc
    16441 cctttcctac ggaaagcgat cataccgaac tctatcctcg ccgggttcat cggattcatc
    16501 ctcggcccga acgtgctcaa ttgggttcat ttcgatgtcg agacattggg gcagatgatc
    16561 taccatctga cggccatagg gttcatcgcc ctaagtctga gaaaggtcac ccgtggaaaa
    16621 aacaccgtcc aatacgtgaa tgcggggatg atcatcgtcg cgacttacct gtttcaaggc
    16681 attttcggat ttttactctt ttgggggtgg cacgcgctag aaccccaagt gacgcccatc
    16741 atcggttttc tcgtgccgat ggggtatggt caaggtcccg gtcaagcctt ttcgatcggc
    16801 acacaatggg aaggtttggg gttggcgaac ggcggcgcgc taggtctggc gatagcggct
    16861 gccggtttcg cttgggcgac gcttgggggg atcttcatcc tgaacgtcgt gctgaagaga
    16921 aagaaattgc gtgactcagt ccagttcatc gggaaaaagc cgatccaggt ccaagatttc
    16981 gagttttctg atatcgatgg gttgaccgta caactcgtta tgatcgtgtc cgtgtatatg
    17041 atcgtctatg gcgccctctt cctcctcacc cgttatgttt tagatttctt cccgatcggt
    17101 aagacgttag gccccgtcct ttggggattc catttcgcat ttggagcggc cgcggcggtg
    17161 tttttcagaa aagggtacga agtgctccac gaaaagaaga tcgtgcacga gaactatctg
    17221 aacgactttt tgttgcatcg gatcgccggt ggcgtttttg atttcatggt agcggcttcg
    17281 atcagtgcca tcgccttgcg aaagatcacc gatgttctgc tggcgctctt cgtcatatgc
    17341 ttcatcgctg gagttttgac atatttcttt gtggcgtggc ttgtgaggaa aacgatgaaa
    17401 gaaaagcagc ttgaaaatat cttggccctg ttcgggaacc tcaccggaac catctctacg
    17461 ggaatggcct tgttaagaga agtagacccg ggcttggaat ccggggcgtc ggataatctc
    17521 gtcttcggta gcggcgtcgc catcttcatc gggataccga tgctcctggt gctatccctg
    17581 ccccccttcg ttttgtccac ggggaatcag gcctattact ggtatgcgtt tttgctgatg
    17641 gtcggctttt tcttgggaat gtttctcctg tggttccgac cacgcttcaa gagaaatccc
    17701 tgaagaacac gcccaagggc gcttgatata tggaggagga atgtgatgta cacgatcgtt
    17761 ttacttagac acggggagag cgtttggaac aaagagaacc tgtacaccgg ctggaccgac
    17821 gtcggtctct cggaaaaagg ggtcgaggag gcccgctcgg cggggcgggt tctcaaacaa
    17881 ctagggtatg aatttaatat ggcctttacg tccgtgctca aacgggcgat actgaccttg
    17941 gaattggcgt taaccgagat ggatcagctg tggataccgg tcgaaaagtc ttggaggctg
    18001 aacgaacgcc actacggggc actgcaagga ttgaacaaag cgcaaacagc acaggaatac
    18061 ggggaacaac aggtgaaact ctggcggcga agttacgatg taccgcctcc cgttttaaca
    18121 cccgacgacg cgcgataccc ggggaatgat cccagatatc aggatctgga cccacgggta
    18181 ctcccgctct ccgaatgcct aaaagacacg ttggagcgcg ttttacccta ctggcacgat
    18241 cgcatcgcgg tcgaaatcaa aaagaaccga aagattctca tcgctgcgca cggcaacagc
    18301 ctgcgcgcgt tggtcaaata cctcgacagg attccggacg acgagatcgt cgagctcaac
    18361 ataccgaccg gcgttcccct cgtttatgaa ctgaaaaaag acttgacacc gattcggcac
    18421 tactatctgg gcgacgaaga aacgatcagg aaagcgcagg aagcggtgaa aaatcaaggt
    18481 aaagctaaag gatagcgggc gggatagtcg agagcgcccg gtcagttggt gtgggtcaag
    18541 ttacggggtg acatagctaa gaggttaatc gcggggtaac cagcgggctg acttcggaag
    18601 cccacgaact ctcgcgatcc gccctttgcc tttttttagc cgcgataggc ctgtggggtt
    18661 atcgcggggt aagtggtttt tacgaccccg cgatttggca ttctcgtgtg ttatcgcgca
    18721 gtaactaaca ggactgcgcg atccgttctt tcccattgtt tctattgaaa gtaacagtat
    18781 cgggcagcca gaaagtacca aaacgtatcg cgccgtcgcg aaaagaaggg gcgcttacac
    18841 cctgatgctc gcgtggggtt tcgcggggta agtggttttt acgaccctac gagaaaatct
    18901 gccaacttaa aagttggcgt tcccaaggcg ttcacaaaag tctttgctct gcgttctctg
    18961 tgctctctgt gcgccccccc cttttttcgc gccgtcgcga aaagaagccg cgcttacgcc
    19021 gcgttgacta cgtggattaa aaatcgtgcg attgaatctg gccttgtttc ttggctcttc
    19081 gatacattac ttgaagccca ctgatcatcc aagaaataca aggtaacata agaggaatag
    19141 aaagagtctg cgggatggcc gattatgcga ttagagcgga ttagcgaaac ggtctaaggg
    19201 tctacagaat tattacgata gaagatgtta attaagtacg tcttgtaaat agtcgaagag
    19261 cctgtttctt gattgcaccc catagcgtga cccacacggt caattgacat tttcggttcg
    19321 gtatggtaga atacaccgcg tgagacaggg tgtgtagctc agtgggagag cgcttccttg
    19381 acacggaaga ggtcgaaagt tcaatccttt ccacacccac caaaaaaggg tcccgta
//
LOCUS       DAOPXN010000021        19398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2851_length_19398_cov_5.498888, whole genome shotgun sequence.
ACCESSION   DAOPXN010000021 DAOPXN010000000
VERSION     DAOPXN010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 19398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19398
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2851_length_19398_cov_5.498888"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PKM49_02200"
     CDS             <1..454
                     /locus_tag="PKM49_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNY81660.1"
                     /translation="YSYIAPEAFFLVKKEIASYPIVGVLAKRMAVFIDRDDPRQAVYA
                     LRAALDLLRKGHILALFPEGTRSLDGRIGPFAKGSLRIAMMTKSLIVPLVITGNENIM
                     PKGTVFIKRAKVKARILPPVDASLYKSEEELVDVVRGGMIEALKELVE"
     gene            659..1015
                     /locus_tag="PKM49_02205"
     CDS             659..1015
                     /locus_tag="PKM49_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY81661.1"
                     /translation="MMACSRTWRNTIGFEKAALKVRAYHGVEGAFSIRACYGCIDPDC
                     ARACPTGALTPRKGGGVQLDESLCIHCGLCIEACAPSALQWDFSTRIPLPCRHCGVCV
                     SFCPNHVLEMMEVDQR"
     gene            1012..2772
                     /locus_tag="PKM49_02210"
     CDS             1012..2772
                     /locus_tag="PKM49_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNY81662.1"
                     /translation="MKPIMMTVDLTTGTSQITPVDELFDTYLGGTGAATKLLTDEIAL
                     NKDPYAPPSPVFFAIGPFNGLYPLCTKTVALFISPLTGNLGESHAGGNLAMALFQAGI
                     HILKITGRSAFPCYVRIVNHEVSIHRAYSLKGMSALASERVLRERIGNTWKSSIMRVG
                     PAGERESPIACVTVDTSRHFGRLGLGGVFGNKNLKAVIVSGNGGIELGSNKGRYLKFY
                     KNLYDQFVHSPALSKYHDLGTSMNILPLSRINGLPTRNFSQGFFEGAASISGERFADQ
                     LLIQKIACAHCPVGCIHMAILRESFDEKNHQYKANKIAYDYELIYALGSNLSINSPED
                     VLRLLLLIEKQGWDAISMGVTLAWSTEAFMKGVITTRETGGLVFKFGDADTYERILRR
                     VIEGDMEFYRDVEKGTAFLAEKYGGESFAITFGRNEAPGYMTGLHGFLGFSTGVRHSH
                     LESAGYSLDQKKSEIEKPAREWTEDLFREGRWRTVLNSLVICLFARNVFTMPTILEGL
                     AALGMTTWDEEKLQELARKIHVMKYELKNTLGFSFDRERLPEKLTSVFTATGLLDEAE
                     YRQQMRIFEDLLAKDARALT"
     gene            3014..3328
                     /locus_tag="PKM49_02215"
     CDS             3014..3328
                     /locus_tag="PKM49_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY81663.1"
                     /translation="MRISEHPILSFRQNKEIHFTFDGEEVTGYEGDTIVSALHALGIW
                     KLSESPEHRRPRGLFCAIGHCSSCLMRVNGLPNVRSCVTPIEEGMVVESQNPRGDLYD
                     HL"
     gene            3315..4925
                     /locus_tag="PKM49_02220"
     CDS             3315..4925
                     /locus_tag="PKM49_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY81664.1"
                     /translation="MITCDLLVIGGGPAGISAAVAAGKAGLNVLLLEERERLGGQLTK
                     QTHRFFGSVAERAGTRGIAIIKDLETVVSQLKNVEVSLSTTALGIYEDGVATALKGRK
                     MIKIKPKAIIVATGAFEKFLPFENNDLPGVYGAGAVQTLMNIYGVLPARRLLMIGSGN
                     IGLIVSYQLVQAGAEVVGVVEAASKIGGYLVHASKIRRLGIPIYPSCTIKKAIGKERV
                     EGAELVRLDEKWKEIPGSQFTVDCDAICLAVGLNPLIDLLGQSGCLFKYIPELGGQVP
                     VRDESMRTNVPFLYIAGDLSGIEEATAALLEGELAGLNAASQIRPDLNLEARVEEVKG
                     HLRALRAGPVGEKIRAGLSKLTGESALSSPQSGRNTIEELSRTGIASSANLAGVLPDE
                     TRKKRRAYAVIECYQQIPCDPCVHSCPFGAIKPFSDINDLPIVDFDLCTGCGQCIGRC
                     PGLAIFTVDETREGDRAKVTLPYEFIPKPAREATVDLMDRSGKRVGQGVVKAVLNTPK
                     QDKTTIVTVEMDKNLVQEVRHIRVVQNT"
     gene            4922..5203
                     /locus_tag="PKM49_02225"
     CDS             4922..5203
                     /locus_tag="PKM49_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY81665.1"
                     /translation="MNDDKIIVCRCEDIDIQTIRNLIDQGVTSLDEMKRITRCGMGPC
                     QGKTCGPIIAREIAMKTGQSMEEILSTHSRPPFGGVLFQEVLEGIDNEE"
     gene            5193..6338
                     /locus_tag="PKM49_02230"
     CDS             5193..6338
                     /locus_tag="PKM49_02230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007956724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNY81666.1"
                     /translation="MRNKASVVIVGGGITGSATAFSLAKRGVKDIVLIERNELASGAT
                     GRCGAGIRQQWGTYQNCLLARESMKVFENFQEVLETDIDIELKQKGYLLLAYSEKEMD
                     QFRSNVEVQHQLGIPSRLVSCLEAKEIVPFLNTQKMFGGAFCPTDGHANPFLTTKAYA
                     NAAERLGVEILKKTEVKGITVENRRIKGVHTDRGFIETDKVVNAAGGWAAHIGEMVGL
                     DLPVYSERHEILVTEPVNPIMDPMVMSFSYNIYCQQTPHGSFIMGYGPENEPRGYNIN
                     ATWQFLEEMVRKATWLLPPLKNLRIVRQWAGLYNISPDRQPIVCESEEVKGFFMGIGF
                     SGHGFMIAPAVGTLLADLVTGSPLTWDVVLDLGRYDRHEIIAEPSVV"
     gene            6376..8004
                     /gene="pruA"
                     /locus_tag="PKM49_02235"
     CDS             6376..8004
                     /gene="pruA"
                     /locus_tag="PKM49_02235"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HNY81667.1"
                     /translation="MNGIHLYEKPVNEPILAYRPGSSERAELKAKLGELKDKHFEIPL
                     IINGKEVRTGQLAPCVAPHDHAKELAVYHKAGEKELRMAIEASINAKPLWESLSWEDR
                     AAIFKKAGDLLAGPWRSTLNAATMLGQSKNAFQAEIDSACELIDFFRFNTYYMTWLYQ
                     EQPYSPAGVWNRSEYRPLEGFVFAVTPFNFTSIAGNLPTAPALMGNTVVWKPASSAVY
                     SAYFIMKLLQEAGLPDGVINFVPGPGAAVGPVVLKHPLFAGVHFTGSTGVFQDMWKTI
                     GGNIAAYRSYPRIVGETGGKDFVVAHPSADPKALITALVRGAFEYQGQKCSAASRAYI
                     PESLWKTIGNPLIEETEGLAMGSPEDFRNFINAVIDASAFKTITGFIDEAKTHPDAKI
                     VAGGTYDSKKGYFIRPTIILANDPRYRTMVTEIFGPVLTVNIYPDSQFEETLTLCDQT
                     SPYALTGAIFAQDRLALVTAERMLRHSAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSAWNLLRWTSVRSIKETFVPPRAYSYPFMEQE"
     gene            complement(8107..8991)
                     /locus_tag="PKM49_02240"
     CDS             complement(8107..8991)
                     /locus_tag="PKM49_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81668.1"
                     /translation="MIKEVVAKGILIPNKHSENWFGAKYTMNIYRGCEHQCIYCDSRS
                     ACYQIENFEDVEVKINALELLHKTLKGKRSKAIVGTGSMSDPYTLCETSYTLTRRALK
                     LIATYGFGIHISTKSDMVRRDLDILKTFQNRVTVAFTITTVDDRLAALVEPFAPSPSR
                     RLAAMKELSDAGIPVGVLMMPILPYIEDSPEGIDRLLESALRHGASFCFWHVGMTCRE
                     GQREYYYQKLDEHFPGLKERYIQRYRDRYVCDSPRLVELHERIRDFCDDNGLITDFDS
                     LLERYPEHRAVQSALFSE"
     gene            complement(8998..9795)
                     /locus_tag="PKM49_02245"
     CDS             complement(8998..9795)
                     /locus_tag="PKM49_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY81669.1"
                     /translation="MRRLRWFFVLFLCLLPVGVYMFWIEPGTLKTTVLDFGQPKSEGS
                     IKIVYFADLHLYRWSRLHDRLLKTIADQRPDCIFFGGDALAKKTNVQDLENFFTQLAK
                     VAPVYAIFGNWEDEAPTHMIQRYQRLGITLIEKDTQLIDVRGITLGVTGLQSHYYLRQ
                     TKHLELLAEANWRILLIHAPNRLEQYPEFYTRFDLVLAGHTHGGQYYIPPFTQTVLKI
                     IGNGALWFRGQYRAKETTVYVTNGVGQWFPGRLFSTPEIVVFSLPPR"
     gene            complement(10852..11412)
                     /locus_tag="PKM49_02250"
     CDS             complement(10852..11412)
                     /locus_tag="PKM49_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNY81670.1"
                     /translation="MNRPVQKYHSTIISGFGGQGVMLIGQTLTSAAMMEKLYVTWIPS
                     YGPEMRGGTANCTVVIDETPIGSPVIDRPTDVIAMNIPSLLKFEKTILPGGTLLLNTS
                     VIDREPTRADIRVYRVNAGEIAGKIGSDKVANMVILGAFVGITRVVSLESVRLALEKK
                     IGEKRPELLAMNLQALKAGENAIAGQ"
     gene            complement(11409..12164)
                     /locus_tag="PKM49_02255"
     CDS             complement(11409..12164)
                     /locus_tag="PKM49_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNY81671.1"
                     /translation="MKGIVAFKRPDALTSKEFTYCPGCLHGVAHRLVAEILDEMKLTD
                     QTIMVAPVGCSVFAYEFFDVDGTVAPHGRATAVATGIKRSLPDRVVFTYQGDGDLAAI
                     GTAETIHTANRGEKITTIFINNAIYGMTGGQMAPTSLVGMKTTTSPYGRKADSEGYPI
                     HVCELLQNLNGVAYLERVTLLTPRDIITAKKAIKKAFTAQVRGLGYSLVEIVSTCPTN
                     WGLSPVESLEWAKQKMLPEFPLKVFIDKVGDVK"
     gene            complement(12161..13228)
                     /locus_tag="PKM49_02260"
     CDS             complement(12161..13228)
                     /locus_tag="PKM49_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNY81672.1"
                     /translation="MAEKILLKGNEAMAEAALRAGCQVYFGYPITPQSELIEYMAKRM
                     PEIGRTFLQAESEVAAVNMIYGAACTGTRVMTSTSSPGFSLMMEGVSYIAGANLPAVF
                     INVVRGGPGLGDIQPAQSDYFQGTKGGGHGDYRLIVLSPATIQEAVNMMFDAFDLADR
                     YRNPVLIIADGLLGQMMEPVELPDFITPDRIPNHEGWALRGKGSREQHTITSFDLDPV
                     KLEEMNLKNYETYQKIQENEVRYEEYLMDDAEVAFVAYGTMGRILKTTIRLAREAGIK
                     AGLIRPISLWPFPEKPIREAAEKVKMVMSVEMSMGQMVEDVRLAVSGKVPTPFYGRTG
                     GIVPTPTEVFEALKREWEALV"
     gene            complement(13240..13467)
                     /locus_tag="PKM49_02265"
     CDS             complement(13240..13467)
                     /locus_tag="PKM49_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY81673.1"
                     /translation="MARKAYIEIDPERCKGCGLCVNVCPQKIIQFSKEFNAKGYHPAQ
                     QVDPDERCVGCGFCYMSCPDTCITVYRIPKV"
     gene            complement(13538..14236)
                     /locus_tag="PKM49_02270"
     CDS             complement(13538..14236)
                     /locus_tag="PKM49_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobQ"
                     /protein_id="HNY81674.1"
                     /translation="MIDFNTHPILKTYKNFIFFGMFGSGKTEISLSFVRTLKTLFELV
                     AIADIDTVSPFFRSRDEKAPLEAAGIRVMVPPFPFLHGDLPIIVPEVGGFLQNEAYRV
                     VVDVGGDDDGATVLGSLSRFAKEHPTAVFFVVNTKRPFSETPNAIIHNMYRLSQKSRM
                     SITAIIHNTHLQAETTLDMIAQGEAILREVSEATKVPVFATVLQDSLARAVSDHALSF
                     PTMTIHRYLKTSWE"
     gene            complement(14249..15331)
                     /gene="buk"
                     /locus_tag="PKM49_02275"
     CDS             complement(14249..15331)
                     /gene="buk"
                     /locus_tag="PKM49_02275"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNY81675.1"
                     /translation="MSHLILVINPGSTSTKLAIFEEDRLIVEKTLRHESKELEPFARM
                     ADQYAFRNQMILDFLKEAGYRVPQFSAVIGRGGLVRPIAGGTYRVNEGMLEDLRTGRY
                     GEHASNLGAILAAELVGDLPIPTLIADPVVVDERIPVACYSGYPSIQRKAIFHALNQK
                     AVARAVAKTLGKTYDRCNLIVAHMGGGISIGAHQNGKVIDVNNALDGDGPFSPERSGT
                     LPLTQLIDLCFRGDISLTEMRKKVKGKGGLVAYLGTTDARAVQQRIRDGDAYAKEVYH
                     AMAYQIGKWIGKMAAVLKGKVDQTVLTGGLAYDQAFLVPWISEMVEYIAPITVIPGGD
                     EERALAESALRVLLRQEEAKVYDPKG"
     gene            complement(15331..16245)
                     /locus_tag="PKM49_02280"
     CDS             complement(15331..16245)
                     /locus_tag="PKM49_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HNY81676.1"
                     /translation="MKEKRIRRLEELLPLARGNKKVVAIAAAEDDTVLKAAQKAYQQG
                     IADFILFGDPHRIRSIAQEEKIDLTPFEIVPATRPEEAGYLAVKSVFEKKAQTVMKGN
                     CKTADLLKIILKDEFELKTDRTMSLVAVFEIPDFERLLIVTDPGIVIQPTLEQKVHLL
                     QNACAVARVLGIEEPKAGVIGAVEVVNPKMPATMEAAVLSKMAQRGQIGKLIVDGPFA
                     LDNVVSEEACRHKGIVSPVGGKADIILTPDLNAGNVLYKALVFLAKAKLATTIMGAKT
                     PIILTSRADSDETKLYSIALNILLSEVL"
     gene            complement(16251..17336)
                     /gene="buk"
                     /locus_tag="PKM49_02285"
     CDS             complement(16251..17336)
                     /gene="buk"
                     /locus_tag="PKM49_02285"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HNY81677.1"
                     /translation="MPLILVINPGSTSTKVGVFKLTSVGPLEPVSKKTISHDADSLRK
                     YPRLLDQIQMRTEDIERFVEESGFSLDQFDAYAARGGILPPMTGGTYEVNAEMVHYLK
                     ELSPVDHASNLAAVIAYGFAEKTGKKAYITDPVSTDEFEAPARYSGIPEIERKSFIHA
                     LNMKAVSRIVSKEIGKPYDQCRFVVAHLGGGISVGALKNGRFIDVNNANDEGPFSPER
                     TGELPVGDVVKQAYSGDYTAKSLKNAYTKRGGLVAYLNTNDLRAAFEMAKRDSQAAEV
                     IEAMVFQIAKEIGGMCAVLQGKIDAIILTGGMAHLIPFVEKIKEYVYNFGLVTVVPGE
                     DELKALAEGTFAVLGGTETAGHFAITR"
     gene            complement(17340..18608)
                     /locus_tag="PKM49_02290"
     CDS             complement(17340..18608)
                     /locus_tag="PKM49_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNY81678.1"
                     /translation="MDNLTEVTDQIVERKLSAVAKRMKASIIREMLKSTSIPGMISFG
                     GGVPDPETFPREHLAEIAKECVEAEYKATLQYGTTEGDKQLKQQYINYLRKYEGIEGL
                     GEDNMVITTGSQQALDLVGRTFLDTESTCAICDPVYLGAASAFMIHAPNFIKLTMQED
                     GPDVDFLESSLKKMTEAEKERFKFFYLVSNFDNPTGICLSLEKRLRILEIAATHKILI
                     VEDDPYGLLRFEGEKIPSIFKLAVERGMQDYVLLLNTFSKILSPGLRIGIVIGNSKIV
                     RKLVVGKQVADLCTSPLLQRMTARYLERHDPVLHLQNALKVYAKKRDTMMASFKEDFG
                     NIEGVHWTYPQGGLFSWMCFPEWVDTMEMLEFATKNLLIYVPGIAFSVGESNKNCMRL
                     SFCLPSEEQIREGSKRLLKAFQQYSATKGR"
BASE COUNT         4377 a         5235 c         5332 g         4454 t
ORIGIN      
        1 ctatagctac atcgcgccgg aagccttctt cctcgttaaa aaggagatcg cctcctaccc
       61 gattgtcggc gtccttgcga agcggatggc ggttttcatc gaccgagatg atccgcgtca
      121 ggcggtctat gctctacgcg ctgcgttgga ccttcttcgg aaaggccata tcctcgcgct
      181 ctttccggaa ggcacccgca gtctggacgg ccggataggc ccgttcgcca aggggagcct
      241 ccgaattgcg atgatgacga agtcgctaat cgtaccgctc gtcatcaccg ggaacgaaaa
      301 catcatgccg aaggggacgg tctttatcaa acgggcgaag gtgaaagcgc gcatccttcc
      361 ccccgtggac gcgagtctgt ataagagcga agaggaactc gtcgacgtgg ttcggggcgg
      421 tatgatcgaa gccctaaaag agttggtcga gtagggtggt ctttccacgc aggaaatgcg
      481 acggccagaa attgatatga cgggggtttc cgcgcgtcgt ttcgtggaaa gaacgggcgg
      541 agtcctcttc tgtcaacgct cgagcccata ggatcggacg acccctaagg aggtttggct
      601 tatcgcaaag ctgttgagaa tcgttcaacg agaacagtgc atcggatgcg gttgctgtat
      661 gatggcttgc tctagaacgt ggcggaacac gatcggcttc gaaaaagcgg cgttgaaggt
      721 acgcgcttat cacggcgtag aaggcgcttt ctctatccgc gcgtgttacg gatgtatcga
      781 tccggattgc gccagagcct gcccaacggg ggcgctcacc ccacgaaagg gaggcggggt
      841 ccagttggac gaatcgctgt gcatccattg cggtctttgt atcgaagctt gcgcgccttc
      901 cgcgctccaa tgggactttt ccactcgtat cccgttgccg tgccgccatt gcggcgtgtg
      961 cgtgtcgttt tgtccaaacc acgttctgga gatgatggag gtggatcaaa ggtgaaaccg
     1021 ataatgatga ccgtcgactt gacaaccggc acctctcaga tcacacccgt cgacgagctg
     1081 ttcgatacct acctgggtgg aacgggggcg gccacgaaac tgttgaccga tgaaatcgcc
     1141 ctgaacaagg atccttatgc acccccctca ccggtatttt tcgccattgg acccttcaat
     1201 ggcctttatc cgctgtgcac gaaaacggtc gccctgttca tctcccctct gacgggtaat
     1261 ctgggagagt ctcacgcggg cgggaacctg gcgatggcgt tgtttcaggc cggaattcac
     1321 atcttgaaaa tcacgggcag atcggctttt ccttgttacg ttcggatcgt caaccacgag
     1381 gtctccatcc atcgggccta ttcgctcaaa ggcatgtcgg ccctcgcctc ggaacgggtg
     1441 ttgagagaac ggatcggcaa cacctggaaa agctcgatta tgcgggtcgg ccctgcggga
     1501 gagcgcgagt cgcccatcgc ctgtgtcacc gtcgacacct cccggcattt cgggcgattg
     1561 ggtttgggcg gggtatttgg caataagaac ctgaaagcgg tgatcgtttc cggaaacggc
     1621 ggaatcgagt tagggtcaaa caaaggccgt tatctcaagt tttataaaaa cctctacgat
     1681 cagttcgtcc attcgccggc gctgagcaag taccacgatc tcggcacttc gatgaatatc
     1741 cttccgctga gccgcatcaa cggcctaccc acaagaaatt tctcacaagg ctttttcgag
     1801 ggagcggcat ccatcagtgg cgaacgcttt gccgaccagt tgttgatcca gaagatcgcc
     1861 tgcgcgcact gcccggtcgg gtgcatccat atggcaatct tacgggaatc cttcgatgaa
     1921 aaaaaccatc agtacaaggc caataagatc gcctatgact atgaattgat ctacgcgtta
     1981 ggctccaacc tctcgatcaa cagtccggaa gacgtgctcc gtttgctcct gctcatagaa
     2041 aagcagggct gggacgccat cagtatgggg gtcaccctcg catggtcaac ggaagccttt
     2101 atgaaagggg tgatcaccac tcgagagaca ggagggttgg tctttaagtt cggagatgcc
     2161 gacacctacg aacggattct tcggcgcgtg atcgaaggag atatggagtt ctaccgagat
     2221 gtagagaaag ggacggcttt tctcgccgag aagtacgggg gcgagtcttt cgcgatcacg
     2281 tttggaagga acgaggcgcc gggatatatg accggactcc acgggttttt gggcttttcc
     2341 accggcgttc gccattccca tctggaatcg gccgggtact cgttagacca gaaaaaaagc
     2401 gagatcgaga agcccgcgcg agagtggaca gaggacttgt ttcgcgaagg acgatggcgt
     2461 accgtgctga actcgctggt gatctgtctc ttcgcccgga atgtgttcac tatgcccaca
     2521 atattggaag ggttagccgc tctgggaatg acgacatggg acgaagagaa actgcaagaa
     2581 cttgcaagaa aaatacacgt gatgaagtac gaattgaaaa acacgttggg tttctctttt
     2641 gatcgagaac ggttgcccga aaagcttacc tcagttttca cggcgaccgg cctcttggat
     2701 gaagccgaat atcggcaaca gatgcggatt ttcgaggatt tgttggcaaa agacgccaga
     2761 gcgttaactt aacgttaatt aaaatagtcg aataccgaca ccctcattct gatacaattg
     2821 tttgaaaggg gaagaagcgg tcggctcatc ctcttgcgga ttctggagaa gcgcccgggg
     2881 caggtcaagg tgacgcacag aaatgaaaat aggaaatatc aatcggaata acaaaacgac
     2941 gtcatttttt tgttattccg atttttcgag agtataacgg taatggtggc tatcactcgt
     3001 atggaggtgt accgtgcgta tcagtgaaca tccgatcttg agttttcgcc agaacaagga
     3061 gatacatttt acgtttgatg gggaagaggt gaccgggtac gaaggagata cgatcgtttc
     3121 cgctttgcac gcgttgggca tttggaagct gagcgagagc ccggagcatc ggcggccgag
     3181 gggacttttt tgcgcgatcg gtcattgctc ttcatgcctc atgcgggtga acggccttcc
     3241 gaacgttcgg agctgtgtca ccccaatcga ggagggtatg gtcgtagaga gccaaaaccc
     3301 cagaggagac ctgtatgatc acctgtgact tgctcgtcat cggtggaggg cccgctggga
     3361 tcagcgccgc cgtggcagct ggcaaggcgg ggttgaacgt gttacttctg gaggagaggg
     3421 agcgcctggg ggggcaactc accaaacaaa cgcatcgatt cttcggatcg gtcgctgaac
     3481 gcgcgggaac acgcgggatc gcgatcatca aggatttgga gacggtagtt tctcagttga
     3541 agaacgtcga ggtctcgctt tcgaccacgg ccttgggtat ctatgaagac ggagtcgcaa
     3601 ccgccctgaa aggcagaaag atgatcaaga tcaaacccaa agccatcatc gtggcgaccg
     3661 gcgccttcga aaagttcctg cccttcgaga acaacgatct ccccggagta tacggcgcgg
     3721 gagccgtgca aacgctgatg aacatctacg gtgttcttcc ggctcggcgc cttttaatga
     3781 ttggatccgg gaatatcgga ctgatcgtct cttatcagtt ggttcaagcg ggcgcggagg
     3841 tagtcggcgt ggtggaagcc gcgtcgaaga tcgggggcta cctcgttcac gcctctaaaa
     3901 ttaggcggtt gggtattccg atataccctt cctgcacgat caagaaagcc ataggcaaag
     3961 aacgagtgga aggggcggag ctggtccgtc tcgacgaaaa gtggaaagag atccccggaa
     4021 gccagttcac cgttgactgc gacgcgatat gtctggccgt cgggctgaac cctttgatcg
     4081 accttttggg tcaaagcggt tgtctattca aatacattcc agaactggga ggccaggtcc
     4141 cggttaggga tgaatcgatg cggacgaacg ttccgtttct ctacatcgca ggcgaccttt
     4201 ccggtatcga agaagcgacg gcagccctgt tggagggtga gctcgccggg ctcaacgccg
     4261 cgtcgcaaat tcggccggat ctgaatctcg aagcccgtgt ggaagaagtg aagggacact
     4321 tgcgtgcctt gcgcgccggg ccggtaggcg aaaaaatccg ggcgggcttg tcaaaactca
     4381 ccggcgagag cgcgctatcc tcccctcagt ctggacgcaa cacgatcgaa gagctctctc
     4441 gcaccggaat cgccagctcg gccaacttag cgggcgtgtt accggacgaa acgcgaaaaa
     4501 aaaggcgcgc gtacgcggtc atcgagtgct atcagcagat tccgtgcgac ccatgcgtgc
     4561 atagctgtcc ctttggcgcg atcaaaccgt tctctgacat aaacgatctt ccgatcgtgg
     4621 acttcgatct ctgcaccgga tgcggacagt gcatcgggcg ctgtcccggg ttggcgatct
     4681 ttacggtgga tgagacgcgc gagggcgacc gcgcgaaggt gacgttgcct tatgaattca
     4741 tcccaaagcc cgctcgtgaa gcgacagtgg acttgatgga ccgttccgga aagcgcgtcg
     4801 gtcaaggggt cgtgaaagcg gtactcaaca cgccaaaaca ggataaaacc acgatcgtga
     4861 cggtagaaat ggacaaaaac cttgtacaag aggtacgcca tatcagggtg gtgcaaaaca
     4921 catgaacgac gataaaatca tcgtttgccg ttgtgaagac atcgatatcc agacgattcg
     4981 caatctgatc gaccaaggcg tgacttcgct cgatgagatg aaacggatca cacggtgcgg
     5041 gatgggccct tgtcagggga aaacctgcgg cccgatcatc gcgcgagaga tcgcgatgaa
     5101 aaccggacag tcgatggaag agatcttgtc cacacactcc cgtccgccgt tcggcggggt
     5161 cttgtttcaa gaggtattgg aagggatcga caatgaggaa taaagcatcg gttgtcattg
     5221 tcggcggagg aattacggga agcgccaccg ctttcagttt ggcgaagcgg ggagtgaaag
     5281 acatcgtgct gatcgagcgc aatgagctgg cctcgggcgc gacgggaaga tgtggggcgg
     5341 ggatccgcca acagtgggga acctatcaga actgcctgct cgctcgagag agtatgaaag
     5401 tcttcgaaaa ctttcaagag gttttagaaa ccgacatcga tatcgaattg aaacagaaag
     5461 ggtatctcct cctcgcttat tccgagaaag agatggacca gtttcggagc aacgtggagg
     5521 tgcagcacca gttgggcatt ccgtcacgat tggtttcgtg tttggaggcc aaagaaatcg
     5581 ttccgttttt gaatacacag aagatgttcg ggggcgcctt ttgtcctacc gacggccacg
     5641 cgaatccctt tttaacgacg aaagcttacg cgaacgcggc cgagcgcctc ggcgtcgaga
     5701 ttttgaagaa aacggaagtc aaagggatca cggttgaaaa ccgccggatt aaaggggtgc
     5761 atacggatcg gggattcatc gagaccgata aggtggtcaa cgccgccgga ggttgggcgg
     5821 cgcatatcgg agagatggtg ggactagacc tacccgttta ttccgaacgg cacgaaattc
     5881 tcgtcaccga accggtcaac ccgattatgg acccgatggt gatgtccttt tcttacaaca
     5941 tctattgcca gcaaaccccg cacggcagtt tcatcatggg atacggaccc gaaaacgaac
     6001 cgcgcggata caacatcaac gcgacctggc agttcctgga ggagatggtg cgaaaagcca
     6061 cttggctcct accgccttta aaaaacctgc gtatcgtgcg gcaatgggcg gggttataca
     6121 acatatctcc ggaccgtcag cccatcgttt gcgaatcgga agaggtcaag gggttcttta
     6181 tgggtatcgg tttcagcggc cacggattta tgatcgctcc cgcggtcgga acgttgctgg
     6241 ccgacctcgt gaccggtagc ccgctgactt gggatgtcgt tttggatctc ggccgctacg
     6301 accgccacga aatcatagcg gaaccatccg tcgtataaaa cggtcgcgag cgcatcagcg
     6361 cataggaggg aaaagatgaa tggaatccat ctgtacgaaa aaccggtcaa tgaacccata
     6421 ttggcctaca gacccgggag ctcggaaagg gccgaactga aagccaaact cggcgagctg
     6481 aaagacaaac atttcgaaat cccgctgatc ataaacggca aagaggtccg caccggtcaa
     6541 ctggctccct gtgtggcccc gcacgatcac gcgaaggagc tcgccgtcta ccacaaggcg
     6601 ggggaaaaag agctgagaat ggccatagaa gcgagtataa acgccaaacc tctatgggag
     6661 tctctctctt gggaagaccg cgcggcgatc ttcaaaaaag ccggcgactt gttggcggga
     6721 ccctggcgaa gcacgctgaa cgcggcgacg atgttgggcc aaagcaaaaa cgccttccaa
     6781 gccgaaatcg attcggcttg cgagctcatc gacttctttc gcttcaacac gtattatatg
     6841 acctggctct atcaggagca accctacagc ccggcaggcg tctggaatcg cagcgagtat
     6901 cggccgttgg aaggattcgt cttcgcggtg accccgttca acttcacctc gatcgcgggg
     6961 aacctaccta cggcgcccgc gttaatgggg aataccgtgg tctggaagcc ggcgtcatcc
     7021 gcggtctatt ccgcctattt cattatgaaa cttctacagg aggcaggctt gcccgacggt
     7081 gtcatcaatt tcgttcccgg tccaggcgcc gcggtcggtc cggtcgtcct caaacatccg
     7141 ctgttcgcgg gcgtccactt cactggctcc accggggttt tccaagatat gtggaaaacc
     7201 ataggcggaa atatcgccgc ttaccgttcc tacccccgca ttgtgggaga gactggcgga
     7261 aaagacttcg tcgtcgctca cccttccgcc gatccgaaag cgctcatcac cgccctggtg
     7321 cgaggggcct tcgaatacca agggcagaag tgttcggccg catccagagc ctatatcccg
     7381 gaaagcctat ggaaaacgat cggcaacccc ttgatcgaag aaaccgaagg actcgcgatg
     7441 gggagccccg aggacttccg caacttcatc aacgctgtga tcgacgcctc cgctttcaaa
     7501 acgatcaccg ggttcatcga cgaggcgaaa acccatccgg acgcgaaaat cgtcgccggg
     7561 gggacctatg actcaaaaaa ggggtacttc atccgcccga ccatcatcct ggccaacgat
     7621 ccgcgctacc gcacgatggt gaccgagatt ttcgggccgg ttttgactgt caatatttat
     7681 ccggactctc agtttgagga gacgctaacc ctgtgcgacc aaacctctcc ctacgcgttg
     7741 acgggagcca tcttcgccca ggaccggctc gccctcgtca ccgccgaacg aatgctcagg
     7801 cattcggcgg gcaatttcta catcaacgac aaacccaccg gcgccgtggt ggggcagcaa
     7861 ccctttggag gggcccgcgc ctccggcacg aacgataagg ccggcagcgc ttggaatttg
     7921 ttgcgctgga cgtcggtgcg ctcgatcaaa gaaaccttcg tacccccgag ggcctattcc
     7981 tatcccttta tggagcaaga gtaagccgac cgcctcgacg cgaaccgcga acgagaggtt
     8041 ggctaacaga atgaatcttg acaacggtga cgccgctaag aaaagggcgt caccgttttt
     8101 tctcgattat tccgaaaaca gggcgctctg cacggcgcgg tgttcggggt aacgttcaag
     8161 aaggctatcg aaatcggtga tcaagccgtt gtcgtcgcag aagtcgcgaa tcctttcgtg
     8221 cagctccacc aatcgaggag aatcgcacac gtaccgatcg cggtatcgtt gtatatagcg
     8281 ttctttcaaa cccggaaaat gctcgtcgag tttttgataa tagtattcgc gttggccttc
     8341 cctgcaggtc attccgacgt gccaaaaaca aaaagaggcc ccgtggcgga gggcgctttc
     8401 taataaccga tcgatccctt ccggagagtc ctcgatgtac gggagaatcg gcatcatcaa
     8461 gaccccgacc ggaatcccag catcagacag ctccttcatc gcggccaatc gtcgggaagg
     8521 ggacggggcg aagggctcga cgagcgctgc aagacggtcg tcgacggtcg tgatcgtgaa
     8581 cgccacggtc actcggtttt gaaaggtctt taggatatcg agatcgcgac gtaccatgtc
     8641 gctttttgtg gagatgtgga taccgaatcc gtaggttgcg atcaacttta acgcccgtct
     8701 cgtcagcgta taagaggtct cacagagagt atagggatcg ctcatcgagc ccgtccccac
     8761 aatcgccttt gaccgtttgc ctttcagagt cttatggaga agctctaagg cgttgatctt
     8821 cacctctacg tcctcgaagt tttcgatctg gtagcaggcg cttctggaat cgcaatagat
     8881 gcactgatgc tcgcatcctc tgtagatatt catcgtgtat ttcgccccga accagttttc
     8941 ggagtgtttg tttggaatca ggatgccttt cgcaacgacc tctttgatca tcgaggttta
     9001 ccggggcgga agagagaaga cgacgatctc cggagtagag aacagcctac cggggaacca
     9061 ttgcccgacg ccgttcgtca catagacggt cgtctccttg gctcgatact gtcctcggaa
     9121 ccatagggcg ccgtttccga ttatcttcaa gaccgtctgc gtgaacgggg gaatgtagta
     9181 ttgcccaccg tgggtgtgac cggctaaaac gagatcgaaa cgcgtgtaga actccggata
     9241 ctgttccaag cgattcgggg cgtggatcag cagaatcctc caattcgcct cggccagcag
     9301 ctccaggtgt ttggtctgtc gaaggtaata gtgggactga aggccggtga cccccaaggt
     9361 gatcccgcga acgtcgatca attgcgtgtc tttttcgatg agcgtgatac ccaagcgttg
     9421 gtatcgctgg atcatatggg tcggcgcctc gtcttcccag ttgccgaaga tggcgtagac
     9481 gggcgcgacc tttgccaatt gggtgaaaaa gttttccaag tcctgtacgt tcgtcttttt
     9541 tgccagggcg tcaccgccaa agaagatgca gtccgggcgt tggtccgcaa tcgttttgag
     9601 cagcctatcg tgaagccggc tccagcgata gaggtgcagg tcggcgaaat agacgatctt
     9661 tatcgaccct tcggatttcg gttgtccgaa gtccagaacc gttgttttca gggtgccggg
     9721 ttctatccaa aacatataga caccgaccgg taaaagacag aggaacagga cgaaaaacca
     9781 cctcaagcgt ctcatctatc gctccttttt caactttctt tctctcattc cgatagcaaa
     9841 agcaaggtga aacggaacgt tcgcgtgtga gtcaagctat atggttaccc cgcgataaca
     9901 cgagcaggca tcgcggcgta agcgctcctt ctttttgcga cggcgcgata cgcctggaaa
     9961 cgccgaactt cacttcggta tgtcgttagg aaacgccctg ggaacgccga actttagttc
    10021 ggcatgtctt ctgggaacgc caacttttta agttggcatg tcttaacaaa acagggttcg
    10081 cacagagggc acagagaaca cagagaaaag aattttgtga acgcctttgt gggaacctct
    10141 tgagaacgct gaaccacagt tcagtatgta tacagggaac gccctgagac cgccgaactt
    10201 cagttcagca ggtcttctgg gaacgccaac ttttaagttg gcatatttta aaaaagaaaa
    10261 gcagcagacc gtatcgcgaa acttagtgag cttccgaagt cagcccgctg gttactccgc
    10321 tataacacac aggcatcgcg gcgtaagcgc ctcttctttt tgcgagggcg cgataccctt
    10381 ctaaacgtgg cataagacgg atcgcgagac ttcgtgagct tccgaagtca gcccgctggt
    10441 taccccgaga taacacacac aggcatcgtg gcgtcttaaa aaacaaaatg cggatcgcaa
    10501 gacttcgtgg gcttccgatg tcagcccgct gggtactccg ctataacaca caggcatcgc
    10561 ggcgtaagcg ttttttcttt tcgcgacggc gcgatatgcc tgggaacgcc aacttttaag
    10621 ttggcatgtc ctaaaaagaa aaacgacagg gcggatcgcg gggtcccgtt ggttaccccg
    10681 cgataacaca cacaggcatc acggcgtcaa aaaaacaaaa tgcggatcac gagacttcat
    10741 gagcttccga agtcagcccg ttggttaccc cgcgatgaac ctcttagttg tgtcacctct
    10801 taacttgacc cacacaacgt tcgccttgct tatgagaggg gcgctcgtcg attattgacc
    10861 tgctatggcg ttttccccgg ctttcaaagc ctgcaagttc atggccaaga gctccggccg
    10921 tttctcgcct attttctttt ctaacgcgag tcgcacgctc tccagggaaa caacacgggt
    10981 gatcccaacg aaggcgccca ggatgaccat attggcgact ttgtcgctgc cgatttttcc
    11041 cgcaatctct ccggcattga cccgatacac gcggatatcc gctcgggtgg gttcccgatc
    11101 gatcaccgac gtattcaata acagcgtgcc gcccgggagg atagtctttt cgaacttcag
    11161 gagagagggg atgttcatcg cgatcacgtc agtagggcgg tcgataactg gggatccgat
    11221 cggcgtttcg tcaatcacga ccgtgcagtt ggccgttccg cccctcattt ccgggccgta
    11281 ggaagggatc caggtgacgt atagcttctc catcatcgcc gcagacgtca aggtttgtcc
    11341 gatgagcatc acaccctggc ccccgaaacc ggagatgatc gtggagtggt acttttggac
    11401 aggccggttc atttcacatc acccaccttg tcgatgaaga ccttcagcgg gaactccggg
    11461 agcatcttct gcttcgccca ctccaaagac tcgacagggc tgagccccca gttggtcggg
    11521 catgtggaga cgatttcgac gagcgagtac ccgagccctc tgacttgagc cgtgaacgct
    11581 tttttaatcg cctttttcgc cgtgatgatg tctcttggtg tcaagagggt tacccgttcg
    11641 aggtaggcga cgccattcag gttttgaagc aattcgcaga cgtggatcgg gtatccttcg
    11701 gaatcagcct ttcttccata cggtgaggtg gtcgtcttca tgccgaccaa agaagtcggc
    11761 gccatctgcc cgccggtcat cccgtatatg gcgttgttga taaagatcgt cgtgattttt
    11821 tctccgcgat tcgcggtgtg gattgtttcc gccgttccaa tggcggcgag gtcaccgtct
    11881 ccctggtagg tgaagaccac gcggtctggg agcgaccgtt tgataccggt ggccaccgcg
    11941 gtcgctctac cgtggggcgc gacggtaccg tcgacatcga agaactcgta ggcgaatacc
    12001 gagcagccga ccggggctac cataatcgtt tggtccgtca atttcatctc gtccaagatt
    12061 tcggcgacca agcgatgggc gactccgtgc aggcagcccg ggcagtaggt gaactctttc
    12121 gaggttaacg catcgggtct tttgaaggcc actatccctt tcatactaac gcctcccatt
    12181 ctcgtttaag ggcttcgaaa acttcggtgg gcgtgggaac gatcccgccc gtgcggccat
    12241 aaaacggagt gggaactttt ccggaaacgg ccaaccgcac gtcttccacc atttggccca
    12301 tactcatttc gaccgacatc accatcttca ctttctctgc ggcttctctg ataggttttt
    12361 cggggaaggg ccacaaggag atgggtcgga tcaaaccggc tttgatgccg gcttcacgag
    12421 ccagacggat cgtggtcttc aagatgcgtc ccatcgtgcc gtaggccacg aaagccactt
    12481 cggcgtcatc catcaggtat tcttcgtagc gcacttcgtt ttcttgtatc ttttggtagg
    12541 tctcgtagtt cttaaggttc atctcttcca gttttacggg atccagatcg aatgaggtga
    12601 tcgtgtgttg ctccctgctt ccttttcccc tgagcgccca gccctcgtgg ttgggtatcc
    12661 gatctggcgt tatgaaatcg ggaagttcga cgggctccat catttgaccc agtagtccgt
    12721 ccgcgatgat gagcaccgga ttccggtacc gatcagctag gtcgaaggca tcaaacatca
    12781 tattcacggc ttcttgaatc gtggccggcg acaggacgat tagccgataa tctccgtgac
    12841 cgcctccttt tgtgccttgg aagtaatccg attgcgcggg ttggatatcc ccgagtcccg
    12901 ggccacctcg gactacgttg atgaatacgg cgggtaaatt cgccccggcg atatacgaca
    12961 cgccttccat catcagggag aatcccggag aagaggtgga ggtcatcacc cgcgtacccg
    13021 tgcaggcggc accgtagatc atattcaccg cggcgacttc gctttccgct tgtaaaaagg
    13081 ttctaccgat ctcgggcatc cgcttcgcca tgtattcgat cagttcgctt tgcggcgtga
    13141 tcggatagcc gaagtagacc tgacaccccg ctctcaacgc ggcttccgcc atcgcttcgt
    13201 ttccctttaa cagtattttt tcagccataa cgccaccccc tacaccttcg gtatccggta
    13261 gacggtgatg cacgtgtccg gacagctcat ataacaaaac ccgcaaccaa cacatcgttc
    13321 gtcagggtca acttgttgag cgggatggta ccccttcgcg ttgaattctt tggaaaattg
    13381 gatgatcttc tgcggacaaa cgttgacgca gaggccgcat cccttacatc tttcggggtc
    13441 gatttctatg taggcttttc tagccatttc ctcctccttg ttccttagag agattttgcg
    13501 ctcatagtat ctgagacgag aaacagagaa accgctttca ttcccaggat gttttcaggt
    13561 agcgatggat cgtcatagtg gggaaagaaa gcgcgtgatc actgaccgct ctcgctagag
    13621 agtcctgaag gaccgtggcg aataccggga ccttcgtggc ctccgacact tcccgaagga
    13681 tggcctcgcc ttgagcgatc atatcgagcg tggtttcagc ctgtaaatgg gtgttgtgga
    13741 tgatggcagt gatggacatt cggctttttt gactaagccg atacatattg tggatgatcg
    13801 cgttcggcgt ttcggaaaac gggcgtttgg tgttgacgac aaaaaacacg gccgtcgggt
    13861 gttctttcgc aaagcgggat agcgatccta aaacggtcgc cccgtcatcg tcacctccta
    13921 cgtccacgac aacacgatag gcctcgtttt gtaaaaaacc tcctacttca gggacgatga
    13981 ttggcaaatc gccgtgtaaa aacgggaaag gtggaaccat cacccggatg cccgccgctt
    14041 ccaacggcgc tttctcatcg cggctacgga agaaggggct caccgtgtcg atatccgcga
    14101 tcgcgaccag ctcgaatagc gttttaagtg ttcggacgaa ggacaaagag atctccgttt
    14161 tgccacttcc gaacatcccg aaaaagatga agttcttata ggttttaaga atcggatggg
    14221 tgttgaagtc gatcaaactg gtctctcact atcctttcgg gtcatagact ttggcttctt
    14281 cctggcgaag caataccctc agcgcgcttt cggcgagggc cctctcttcg tcaccgccag
    14341 gaatgaccgt gatgggcgcg atatattcta ccatctcgga aatccacggg actaaaaagg
    14401 cctggtcata ggctaagccg ccggtgagca cggtctggtc gactttgccc ttcagtaccg
    14461 ccgccatctt cccgatccac ttgccgattt ggtaggccat cgcgtgatag acctctttgg
    14521 cgtaagcgtc tccgtcacgg attctttgct gaacggcgcg cgcgtcggtc gtgccaaggt
    14581 aggcgaccaa cccccccttc cccttgacct tttttctcat ctccgtcagc gaaatatctc
    14641 cgcggaaaca gaggtcgatc agctgggtca acggcagagt gccgcttcgc tctggagaga
    14701 aggggccgtc tccgtcgagg gcgttgttca catcgatcac cttgccgttt tggtgagcgc
    14761 caatggagat gccacccccc atatgcgcca cgatcaggtt gcaccggtcg taggtttttc
    14821 ccaatgtctt ggccacagcc cgagcgaccg ccttttgatt gagggcgtgg aagatggctt
    14881 ttcgttggat cgaagggtag ccggaataac aggcgacggg aatgcgttcg tcgacgacca
    14941 cggggtccgc gattaacgtg gggatcggca aatctcccac caattcggcg gccagaatcg
    15001 cccctaaatt agaggcgtgt tcgccatagc gaccggtgcg aaggtcctcc agcatcccct
    15061 cgttcacccg gtaggtgccg ccggcgatgg ggcgcaccaa accgccgcgg ccgatgacgg
    15121 ccgaaaactg tgggacccgg taccccgcct ccttcaggaa gtccaggatc atttgatttc
    15181 ggaaggcgta ttgatcggcc attctggcga acggctcgag ttccttactc tcgtgccgta
    15241 gggttttttc tacgattaac cggtcctctt cgaagatcgc cagcttggtc gaggtcgatc
    15301 ccgggttgat caccaaaatc aggtgactca tcacaacacc tcgctcaaga ggatgttcaa
    15361 agcgatagaa tagagcttgg tttcgtcgct gtccgctctc gaggtcaaga taatcggggt
    15421 tttcgcgccc ataatcgtgg tggcgagttt ggctttcgct aaaaacacga gcgctttgta
    15481 caagacgtta ccggcgttga gatcgggggt caagatgata tccgctttcc cgccgacagg
    15541 gctcacgatc cctttgtggc ggcacgcctc ttcggagacc acgttatcca aagcgaaggg
    15601 cccgtccacg atcagcttac cgatctgccc cctctgagcc attttgctga gcactgcagc
    15661 ttccatcgta gcgggcattt tcggattgac aacctccacg gcgccgatca cgcccgcttt
    15721 gggttcttcg atacccaaga cacgggccac cgcgcaggcg ttttgcaata aatgcacctt
    15781 ctgttcgagc gtcggttgga tcacgatacc cggatccgtg acgatgagta gccgttcaaa
    15841 gtccggaatc tcgaaaaccg cgaccaaact catcgtccgg tcagtcttca actcgaactc
    15901 gtcttttagg atgattttta acagatcggc agttttgcag ttgcctttca tcaccgtttg
    15961 cgcctttttc tcgaagacgc ttttcaccgc caaatacccg gcttcttccg gtcgagtggc
    16021 gggaacgatt tcgaaaggag ttaggtcgat cttttcttcc tgagcgatcg atcggatcct
    16081 gtgtggatcg ccgaacagga tgaaatcggc aatcccctgt tgataggctt tttgcgcggc
    16141 cttcagaacc gtatcgtctt cggcagccgc gatagccacg acctttttat tgccgcgcgc
    16201 cagcggcagg agctcttcga gccttctgat tcttttctct ttcatcactc tcacctcgtt
    16261 atggcgaaat ggccggccgt ttccgttccc ccaagaacgg cgaaggtccc ttcggcgagc
    16321 gctttcaact cgtcctcacc aggaacgacg gtcactaagc cgaagttgta aacgtattct
    16381 tttatcttct ccacgaaggg aatcagatgc gccatccccc cggtcaggat gatggcgtcg
    16441 atcttgccct gtaaaaccgc gcacatccca ccgatctctt tcgcgatctg gaagaccatc
    16501 gcctcgatca cttccgccgc ttgagagtcc cgcttggcca tttcgaaagc cgcgcgcaga
    16561 tcgttggtat tgagataggc gaccagaccg cctcgcttgg tataagcgtt ttttagagac
    16621 ttcgccgtgt agtcgcccga ataggcttgt ttgaccacat cgcccacggg gagttcgcct
    16681 gtgcgctccg gactgaaagg cccttcgtcg ttggcgttgt tcacatcgat gaaccgcccg
    16741 tttttcaaag cgcccacgga gatgcctcct cccaagtgcg cgacgacgaa gcggcattga
    16801 tcgtaaggct ttcctatctc tttggaaacg atccgagaga ccgctttcat attcagcgcg
    16861 tggataaagc tcttccgctc gatctcaggt attccggaat atctcgctgg ggcttcgaat
    16921 tcatcggtcg agaccggatc cgtgatgtaa gcctttttac cggttttttc cgcgaagccg
    16981 taagcgatca cggcggccag attcgaggcg tggtccaccg gggaaagttc ttttaggtaa
    17041 tggaccatct cggcgttgac ttcgtaagtg cctcctgtca tcggcggcaa gataccgccc
    17101 cgcgccgcgt aagcgtcgaa ttgatccaat gaaaagccag actcttcgac gaacctctcg
    17161 atatcttccg tgcgcatttg gatctggtct aaaagccgcg gatacttccg aagggaatcg
    17221 gcgtcgtgcg aaatggtctt ttttgagacc ggttctaagg gtccgaccga tgtgagctta
    17281 aagaccccga ccttggtgga ggtagaccct ggattaatca ccagaatcaa gggcatgcat
    17341 catctcccct ttgtcgccga gtactgctga aacgccttca acaatcgttt ggagccttcg
    17401 cgtatctgtt cctccgacgg cagacagaag gataggcgca tacaattctt gttgctttct
    17461 cccacagaaa aggcgatgcc cgggacgtag atcaggaggt tcttcgtcgc aaactcgagc
    17521 atctccatcg tatccaccca ctcagggaaa cacatccagg agaacaaccc cccttgcgga
    17581 taggtccagt gaacgccttc gatgttcccg aaatcctctt tgaaagaggc catcatcgtg
    17641 tctcgctttt tggcgtacac cttcagggcg ttctgtagat gaaggacggg gtcgtggcgt
    17701 tccagatacc gggcggtcat tctctggaga agcggcgatg tgcataggtc cgcgacttgc
    17761 tttcccacca cgagtttccg gacgatcttt gaattcccga ttacgatacc gattctcaaa
    17821 cccggagaca gaatcttgct gaaggtgttc aacaaaagaa cgtagtcctg catgccgcgc
    17881 tcgacggcta acttaaagat ggatggaatc ttttccccct cgaagcgaag tagcccgtat
    17941 ggatcgtcct caacgatgag gatcttgtgg gtagccgcga tttcgaggat gcgaaggcgt
    18001 ttttccaacg ataggcatat cccggtgggg ttgtcgaagt tgctgaccag gtagaaaaat
    18061 ttgaaccgtt ccttctcggc ctcggtcatc tttttaaggc tgctttccaa gaaatccaca
    18121 tcgggcccgt cttcttgcat cgtcagtttg atgaaatttg gcgcgtggat cataaaagcg
    18181 ctggcggccc cgagataaac cgggtcgcag atggcgcaag tcgactcggt gtcgagaaag
    18241 gttcttccga cgaggtccaa cgcctgctgg gagccggtcg tgatcaccat attatcctct
    18301 cccagccctt cgatcccctc gtactttcgg aggtagttga tatactgctg tttcagctgt
    18361 ttgtctccct cagtcgttcc atactgcagc gtcgctttgt actcggcctc gacgcactct
    18421 ttggcgatct ctgcgaggtg ttctctcggg aaggtttcgg ggtcgggcac acctcctccg
    18481 aaggaaatca tacctggaat ggaggtagat ttcagcatct cgcggataat actcgctttc
    18541 atccgtttgg cgacggcaga cagttttcgc tccacgatct gatccgttac ttccgttagg
    18601 ttgtccataa aactctcccc ttttttatga ttttataacg catgctttcg aagaaaaagg
    18661 caacgaacct cggtgaaaac ccctcgcgag cgagaggcgc ctattttcat tgtaccaaaa
    18721 agggagggag ccgtccaaaa aagcagagaa agtgagctat ataataatta ttatatcgta
    18781 tcgctaactt ctgaaaaaac agacgagcgc gaaaaattat gcagtatgca ataaaatgca
    18841 cgcaatacga ctacctgttt tgagtagaac acttcggaac gcgagagact ttcgaatgct
    18901 ttataaggtc gtatgtgcca agttatggag tgtaatcgag aaacaaggtc agaatcgaca
    18961 gtaggatttt tagcccttgt agtcatcgcg gcgtagaagc ggaaaagggc ggatcgcggg
    19021 gtcgtaaaaa ccacttaccc cgcgataacc tcacacgggc atcgcggcgt aagcgccggt
    19081 tcttttcgcg acggcgcaat acactttttc acgtatcttg aaaaacaggg ttcgcacgga
    19141 ggacacagag agaaaaatta tgggaacaac tgggaatgcc catgcgttcc cagctcgttg
    19201 cgctcagagg gtgcgctcct actacccctc ttcacccttc agcacgtaca ccccattcga
    19261 tacgaggagc tctttcgcgg aagacatata gccgttttga gagtatgtcg cgaaaagaat
    19321 tttcggactt tccgcggtaa taacctgctt gatctctatc cagcggtccg cttcgcgttt
    19381 cccatacttc cctttcgt
//
LOCUS       DAOPXN010000022        19225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2897_length_19225_cov_5.017058, whole genome shotgun sequence.
ACCESSION   DAOPXN010000022 DAOPXN010000000
VERSION     DAOPXN010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19225
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2897_length_19225_cov_5.017058"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PKM49_02295"
     CDS             complement(<1..396)
                     /locus_tag="PKM49_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNY81679.1"
                     /translation="MEVLTVTLNPALDREIIIPYFEPNRLHRVFERDKMSLSPGGKGI
                     NVSVILSDWGIDSAATGFLGGNIGRMIEQEILSRYRNITTSFVHIDEESRENIAILDD
                     HNHTLTEINEAGPTIEEQDLAHFLKVFSSL"
     gene            complement(356..2533)
                     /locus_tag="PKM49_02300"
     CDS             complement(356..2533)
                     /locus_tag="PKM49_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HNY81680.1"
                     /translation="MSLPEKKREVFSLIYALDIGTRTVIGVLAEKIDEKIVVYDSEIM
                     EHEERAMSDGAIQDVQKVSQIVKRVTRTLSERNQIELTKASVAVAGRFLKTSIGEAEL
                     NVEEERAISSDTIRLLEMNAIQASMEQLRSDGREMYCVGYSVLFYTLDGEWIKNLQGH
                     RGKVVTVKVIAAFLPAYFVNAMMNVLELSGLTPQHMTLEPIAAINLVVPPDLRKLNIV
                     LADVGAGTSDIAVSRDGTVIAYSVVPMAGDEITESLCERFLLDFQTAEMVKRRISTED
                     VDTVTVYDILDTEVNITREVFIETVAPVIEEITQKIADETIELNGKPPVAALIVGGGA
                     KSFSFVDVLGKKLNLSSHRAALKSIENLPNVEDRSGKLRGSEYITPVGIAYSVDTHTS
                     SVFVRVSINGKSVDLMGIDNKNTVMQALLQCGYRIEEIIGKPGPALTYELNGEVKIVK
                     GTMGQEASIKIEGETGRVQSRLKHGDSVSFKPGKEGKSPELFLSSVVPPIPLVLNGNK
                     ISLMPRVTINGNELTGDVQIKDGDRIETDFLCRAYQIPEYVSDREKKYLSIILNGMIR
                     TFVVREEEVLVGEQLVDSDYVLKPGDQVRVQVFEKTPVVRNLIKDIPVKVVTVLANGK
                     ETQLPVTVFNVRIDGTIANLDSPLYDKAEVQVDTIEKIPKLIDLFSLLEFDLSHARSY
                     ELRVNGKHASFMETIEDGDEIEILLNRGEGFGSIDRHAQSGSR"
     gene            2631..3311
                     /locus_tag="PKM49_02305"
     CDS             2631..3311
                     /locus_tag="PKM49_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY81681.1"
                     /translation="MKDLRLVFDHGGFKVYRATLPPIGVNTYFLTDDTTLLVIDPGTG
                     AFDLVRKGFEQFEEATGILITHTHYDHIAGLREFPRKRIFLSQAAKPGLFSPEKNLSQ
                     QLSKRGLSLSQDEETVLVLSEGPTDVGSLRFETWILPGHTPGDALFDFGCCLFTGDIL
                     FENSIGRTDFPGASERAMSRSLRRLYTYLKTQQPNKPVFPGHMNAFTVRDALERNPYL
                     AIFREIST"
     gene            3335..3928
                     /gene="xpt"
                     /locus_tag="PKM49_02310"
     CDS             3335..3928
                     /gene="xpt"
                     /locus_tag="PKM49_02310"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HNY81682.1"
                     /translation="MDCGVAGVPERLKAMILKEGKVLPNGILKVDSFVNHQIYPALHM
                     DIGRYLSDPFLGLGINKVLTLESSGIAPAFATALTLGVPMVFARKKKPITMQEYWHES
                     APSHTKSGVVDIYVSKEVLSDRNVVLIVDDFLATGLSILALARIVQASGAKLAGIATV
                     IEKTFEGGRELLMKSHQTHIHSAVRISRLSESAISFE"
     gene            complement(3956..4828)
                     /gene="fabD"
                     /locus_tag="PKM49_02315"
     CDS             complement(3956..4828)
                     /gene="fabD"
                     /locus_tag="PKM49_02315"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HNY81683.1"
                     /translation="MKTALLFPGQGSQAPGMGKKVFDEHEPSRRYLENANEILGFDLQ
                     TLIFGEDPEALKPTEIAQPAIYTVSAMYFEFLRSKGHTFDAVAGHSLGEYSALHAAGV
                     FSFSEGLSLVRKRGLLMAGSTEKKGGMAAIIGIPFEHVEQIVAQFLEVWIANINTPNQ
                     TVISGALDQVREAMASAKTAGAKIVVELNVSTAFHTPYMKNAEKELTEAIDRSVFLTP
                     RVPVFSNFTGNYSNDIGQIKENLTRQLTGQVRWSQTLSEMEAFGVEGVYEVGFGNVLK
                     GMLKPFPKLKYLPI"
     gene            5489..6760
                     /locus_tag="PKM49_02320"
     CDS             5489..6760
                     /locus_tag="PKM49_02320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY81684.1"
                     /translation="MRIVEVKTNRERAAFRDFPYKLYKKNDQWCPYFVHEMNHILYHG
                     RSIVYDRSQATFFLAMRDKEVVGRISVGIDEELNAHKRQQHAFFSFFDAIDDQEVGHA
                     LLSKAVEWAKEKGMLYLKGSVSPTNGDDFRGILTEGFQHSSYFLMPFNFEYYKTFFRE
                     WDRYLEYYAFFQDINQPIPPSRFNLMKRILTENLSDKEAALITDERQLTRAFLDGFFY
                     KEGYTIEEVNLNDLSKSACDLYEVFSKSIPDDWEEDLMPFDYAQCEQLVKDFKLTLPP
                     PLCLGVYYKGKAVGISAVLPNYNPIVRKMKGRLFPFGWISLLSSKNRIKEGRAVILFV
                     VPEHQNRGISALLVLKLRENLKRLGYGKIEFSSISAYNTQMIRLAEFIGCKRYKTYTV
                     FGKSLIDRPLTLEETYGTGAETMRRFFKQRP"
     gene            complement(6757..8007)
                     /locus_tag="PKM49_02325"
     CDS             complement(6757..8007)
                     /locus_tag="PKM49_02325"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNY81685.1"
                     /translation="MKKEAFQVAVTGMGTINPLAKDLPSFSKALEEMTIGIDRISAYD
                     ASPYTFQVAAEVSDFDPKMYMDRKRAKKYDRFIQFAVAATRFALEDAALTMDGEWKKQ
                     AGVTISSGIGGLQTLLKAMKDFGDLGPEYISPFLIPMVIPDMASGAVSMEFGLKGPNF
                     ATVSACATSLHAVIMSAMMIKHGYTKLVVTGGSEALIHPLPIGAFGNMMALSRNNQNP
                     KEASNPFDRRRDGFVMGEGAGVLVLEEMEHARQRGARIYGTIEGFGLSGDAFDFSASD
                     PQGEGASRAINQAFEVSGVSPSQVDFINAHATATIIGDISEANSLRSSFGEALKNIPV
                     QGTKSLIGHGLGAAGANELIAAILETQNGFIHGMPSLTQRDEAFTDLNIPEKTVYRKS
                     SVFLKNSFGFGGHNASLIFRAISD"
     gene            8161..8604
                     /gene="fabZ"
                     /locus_tag="PKM49_02330"
     CDS             8161..8604
                     /gene="fabZ"
                     /locus_tag="PKM49_02330"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNY81686.1"
                     /translation="MNIDEIKSAIPHRYPFLMVDRVTQLEPDRIQAYKNVTVNEPYFQ
                     GHFPHYPIVPAVLQLEGIAQTAALLLHDTYKEREGIPLFLGIDSARFLREVRPGDRMD
                     YVVEVKQKMGDFYKVNGQVLVEGQVAARCTLFVGVKFFDPPEAHV"
     gene            8597..9571
                     /locus_tag="PKM49_02335"
     CDS             8597..9571
                     /locus_tag="PKM49_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNY81687.1"
                     /translation="MFDSLPLCRLLGIKYPLILGGMAWVGKAALAAAVSNAGGLGTIG
                     SGSMTIEVLRSEIDRCRALTAKPFAVNLLMLDPDAEAKAALLLELRVPVVVFGAGNPG
                     VFVEAFRRREIVTMGVVSSENLALLLERAGVDVIIGEGCESGGHIGSVTTMVLIPALA
                     DLVRTPIVAAGGIADGRGIAAAFMLGAQGVQIGTRFIASVESDANDTYKQLILKAGVR
                     DTIVTGEDLGHPVRVLKTPFSRKIKKMERQSADEVESLLLGSFRKAYQNGNLNEGSFL
                     AGQSAGLVHDISTCQEIIEKMFAQAATLLEYTIPHINERRKHEQNGSI"
     gene            9549..9791
                     /locus_tag="PKM49_02340"
     CDS             9549..9791
                     /locus_tag="PKM49_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY81688.1"
                     /translation="MNRTEVFSRVKKALVEKLDVDEDQISLDADLRDDLGGSSLELVD
                     LVMDLEREAKIKIELEELRSIQKVKDVVELIQKKLS"
     gene            9856..10143
                     /locus_tag="PKM49_02345"
     CDS             9856..10143
                     /locus_tag="PKM49_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81689.1"
                     /translation="MGGLILYYVFSMFASKQEGYLDEKRTVTAQFEEYRDAKNAYRHF
                     SKQIKAWKRLDPSHIGRCTLIVAPSQSDQSSESTKPRDLLGQQSKLLAERN"
     gene            complement(10215..10793)
                     /locus_tag="PKM49_02350"
     CDS             complement(10215..10793)
                     /locus_tag="PKM49_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81690.1"
                     /translation="MKKRYEENGQKGKMGMEEKSTTYRNSAEPAKRSLILVFGVSLVI
                     LGIFGLLGAFGILSITWERVWPSFLLLPGLIFEIAFFRNKKLSGLLVPGGILITLGLL
                     FFLCSYTDYGILQYLWPIFVMAPGIGLLQMYFLTLPAKKGLFIGGVIPFGIGSLFMFF
                     TLLQQTFFSVAFPLVLIGIGLYFLYRFFKTKS"
     gene            10915..12042
                     /gene="galK"
                     /locus_tag="PKM49_02355"
     CDS             10915..12042
                     /gene="galK"
                     /locus_tag="PKM49_02355"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HNY81691.1"
                     /translation="MLHFKETVFKKERNPMYGYAPGRANIIGEHVDYNDGYIFPFAIE
                     RKTTVLIKKSDDSSFRVRSKGYGAFVINPEKMEPRGDWTDYIQGVFWVLKRHDRFHLF
                     PVTLEVDSDVPIGAGLSSSASVEVATLAALNGFFTLGLDKATLIAYAKEAENDFVGVQ
                     CGIMDQFASVMGKEDHAIFMDTRSGDFEYVPIDLDDKTFLVINSKVSHALGSGEYNRR
                     RAECALCLNTLHRSSFRDISLAELENKRALLPPVAYLRGLHVLTEIERTCLCKTALLE
                     KDWALVGRLLTQSHSSLRDNYQVSCEEIEYLIENLNQFSSVWGARIMGGGFGGSLIAL
                     VEERAVPKILAELSDKYFHRYGIRLEGYQVKPSKGAGYVDS"
     gene            12032..13021
                     /locus_tag="PKM49_02360"
     CDS             12032..13021
                     /locus_tag="PKM49_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family protein"
                     /protein_id="HNY81692.1"
                     /translation="MTVSVAEKREALLGEIGKTIERSSRVAIAFSGGMDSTVAACCVR
                     EALGERGNAVLVHFSFGPYTYEKTAENVRLLAKRIGFPLYLVDKRKELEMLSRKGPSC
                     NRCTKHIKLGGMRDFAKEWRADWIISGANQSDTWGQYGIAVHQNTYSPLFHLEKPEIR
                     ELLDHFGFALSEVRSGESALREGCKLKHLMKAMAVPEYHGEAVCLSNETLLSRLREAR
                     FETQFANVKIIGPLRKNIALINVSPLPPATLREKLVREIGALESISEAAIVDRPVTLY
                     LKANPGIIRSPHSRHWLEVGKIGPEFSGPIRFVWMESPNRSLNTYHVVDYTFA"
     gene            13057..13830
                     /locus_tag="PKM49_02365"
     CDS             13057..13830
                     /locus_tag="PKM49_02365"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY81693.1"
                     /translation="MDYTKIPPETKIVDPRCAPFFHNGSRESILLLHGYTGTPYDMRY
                     LAERLSQQDYAVFVPRLPGHGTNRRDFLQSKDDDWLREAIDGYLTLQAMGKPVHVCGL
                     SMGGVLALLLAERFPIRKVCLCAPAIVVKNPMMKWTPFLRHVVKTTKRPRVEYDEAFL
                     NTLHCEYWSDHLIEKLADLSRLQKRCLKALENVTSDCLVLLARKDTVVPTGVADLLRN
                     RTRANSFRTEILEESGHVILNDSEKEKAADTIIRWLDNR"
     gene            13947..14291
                     /locus_tag="PKM49_02370"
     CDS             13947..14291
                     /locus_tag="PKM49_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81694.1"
                     /translation="MAGYQLFWLCKLCRGDCCRIYDPTRLPKGKSIGEWSDEFHQFRE
                     GYGVLPLFIPEIVHHRTNAHLLEILREKGIDPFACEYLGLEGCLIAWDNRPGQCIAYR
                     CPTFKDYDKDAS"
     gene            14296..14547
                     /locus_tag="PKM49_02375"
     CDS             14296..14547
                     /locus_tag="PKM49_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81695.1"
                     /translation="MIRRVCSIYPQSVTPSPLSHLFAYFRFVGYRFARGCIQVLDGLL
                     SILLAPLGMDTEIYARFIYRVEKYRKPLWNRRYQNPNSL"
     gene            14740..15534
                     /locus_tag="PKM49_02380"
     CDS             14740..15534
                     /locus_tag="PKM49_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81696.1"
                     /translation="MRKTLGIILCLLLSLMAFGDIIYLVDGSKLSGKILGYLGDEVEV
                     LLETGHSVKLRTNQIIRIEPLDTGLLAASNAAIETAPLAAAPAAAAAAAPATPIPAGH
                     WPYQGYSVTGIPEATGYIPATEIPVMFGDPETEMRKRLLMYSEMKKEPWVAAELSLLI
                     PSAGHLYTGEWERGFLFLGAKTAFLGVAIFGFLPQETGEVDSNDKPIMKPQNTLLGAV
                     GAGGFALFTLLEFVDSYYAAERYNQVLRLRLGIERFDPMMLPTFSK"
     gene            complement(15546..15805)
                     /gene="ffs"
                     /locus_tag="PKM49_02385"
     ncRNA           complement(15546..15805)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKM49_02385"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(16519..17844)
                     /gene="xylA"
                     /locus_tag="PKM49_02390"
     CDS             complement(16519..17844)
                     /gene="xylA"
                     /locus_tag="PKM49_02390"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HNY81697.1"
                     /translation="MKDYFPNVEKIGFEGPDSDNPLAFRYYNPEELIGGKKAGEQLRF
                     AVAYWHTFCGEGRDMFGEPFFERSWNRYADPYDRSIAKADAAFEFMSKLGVSYFCAHD
                     RDLAPELDTLRQTNALMDRVIPHIQALMKETGIKLLWGTANLFSSPKYLHGAATSPDA
                     KVFAYAAGQVKKALEVTLALGGEGYVFWGGREGYETLLNTDMKRELDHLAAFYRIILD
                     YAREIRFEGQFLIEPKPKEPTKHQYDFDAAHSLAFLQRYGLEPYFKFNIEANHATLAG
                     HTFQHELRYSRMVGKLGSVDANMGDLLLGWDTDHFPTNMYDTMLAMYEILLNGGLAPG
                     GLNFDAHVRRGSYEKKDLFLSHIAAMDAFAIGLKAAKMMIEHPFLERGIQERYVSFDS
                     GLGAEIEKGPVDIHSLESAIIDTPIPLPASGRQEWLENYLNRLLLSVVR"
     gene            complement(18271..18933)
                     /locus_tag="PKM49_02395"
     CDS             complement(18271..18933)
                     /locus_tag="PKM49_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81698.1"
                     /translation="MNLAIASLIIGILGVVFSLIGLVTSVQSVYAGDIGGLFAGSAIF
                     TLSFLTNVIVLILQTIVIFQWSTSLNMNIDNTRLICRRLTAANPQNSDYRELEYRLDT
                     LKFEPWAFWVYLGLYFLGIVVPAYSWLFNIIGFVFLGIYLQQTFKTAALLQEYKDRFY
                     SVYQNAMATPVRKMKNRNVFLTVLFIIITLGIYWWYLLIAFSKEINEFLELDRRLRSS
                     LG"
BASE COUNT         4794 a         4989 c         4750 g         4692 t
ORIGIN      
        1 cagagagctg aagactttta aaaaatgcgc gaggtcctgc tcttcgattg tagggccggc
       61 ttcattgatc tcggtgagcg tgtgattatg gtcgtcgagg atcgcgatgt tctcccggct
      121 ctcctcatcg atgtgaacga aactcgtcgt gatgttccgg tatctgctca gtatttcctg
      181 ttcgatcata cggccgatgt tacctcccaa aaacccggtc gccgcgctat cgatccccca
      241 atccgaaagt ataacggaca cgttgatgcc tttgcctccg ggagataggc tcatcttgtc
      301 cctttcgaag acacgatgca aacgattggg ttcaaaatac gggatgatga tctctctatc
      361 gagagccgga ttgagcgtga cggtcaatac ttccaaaacc ttcccccctg ttcaatagga
      421 tctctatctc atctccatct tctatggttt ccataaagga cgcgtgtttc ccgttgaccc
      481 tcaattcata gcttcgcgcg tggctcaagt cgaattctag caaagaaaac agatcgatca
      541 gtttcggaat cttttctatg gtgtcgactt gcacttcggc tttgtcgtaa agagggctat
      601 ccaaattagc gatcgtgccg tcgattcgga cgttaaaaac tgttaccggc aactgggttt
      661 cttttccatt ggccagtacg gtcaccactt taaccgggat atccttgatc aaattccgga
      721 cgacgggcgt tttttcgaaa acctgcactc tcacttggtc tcctggtttt aaaacataat
      781 ccgagtccac gagctgttcg ccgactaaaa cctcttcttc cctgacgacg aaggtgcgga
      841 tcatcccgtt caggataatc gatagatact ttttttcacg gtccgaaacg tattccggga
      901 tttgataggc ccggcataaa aaatccgtct ctatcctgtc tccgtccttg atctggacgt
      961 ctccggtcaa ttcgttgccg ttgatggtaa cccgcggcat cagagagatc ttgtttccgt
     1021 tcaaaaccag cggtatcggg ggtacaaccg atgacaagaa aagctccggc gatttgcctt
     1081 cctttcccgg cttgaatgaa acgctatccc cgtgtttcag tcgagattgt acccgtcctg
     1141 tttcaccttc tatcttgatc gacgcttctt gccccatcgt ccccttgacg atcttgacct
     1201 cgccgttcag ctcataggtc aacgccggtc cgggtttgcc gatgatctct tcgatacggt
     1261 atccgcactg taacagggct tgcatcacgg tgttcttatt gtcgattccc atcaagtcga
     1321 cgctcttgcc gttgatactg acacgaacga agacactgct cgtatgggta tccaccgagt
     1381 aagcgatgcc aacgggggtg atatactcgc tgccccgcag cttcccgctt cggtcctcta
     1441 cgttcggcag gttttcgatg ctcttcagcg ccgcgcggtg tgacgacagg ttgagtttct
     1501 tccccaagac atccacaaaa gaaaatgatt tcgctccccc gccgacgatc aaggcggcca
     1561 ccggaggctt cccgttcagc tcgatcgttt cgtccgctat cttctgggtg atctcttcga
     1621 tcacgggcgc taccgtttcg atgaacacct ctcgtgtgat gttcacttct gtatccagga
     1681 tgtcataaac tgtcactgta tccacgtcct ccgtcgagat tctccgtttc accatctcgg
     1741 cggtttgaaa atccaacaga aaacgctcgc atagactttc tgtgatctcg tctccggcca
     1801 ttgggacgac gctgtaagcg atcacggttc cgtctcggga taccgcgata tcgctcgttc
     1861 cggcccctac atccgccaag acgatgttga gcttcctcag gtcgggtggg acaaccaagt
     1921 tgatcgctgc gatcggttcc aaggtcatat gctgcggcgt caacccggac agttccaaca
     1981 cgttcatcat cgcgttaacg aaataggcgg ggagaaaggc cgcgatgact ttcaccgtga
     2041 cgaccttccc acgatggccc tgcaagtttt ttatccactc tccgtccaag gtgtagaaga
     2101 ggacggagta tccgacgcag tacatctctc tgccatccga acgtagctgc tccattgaag
     2161 cttggatggc gttcatttcc aacaaacgga tggtgtccga agagatcgcg cgctcttctt
     2221 ccacgttcaa ctccgcttct ccgatagagg tctttagaaa tcttccggcg accgcgacag
     2281 aggctttcgt caactcgatt tgatttcttt cggagagcgt tcgggttacc ctttttacga
     2341 tttgagacac cttctgcaca tcttggatag cgccgtcact catcgcgcgt tcttcatgtt
     2401 ccataatctc cgagtcgtaa accacgatct tctcatcgat tttttcggct aaaaccccga
     2461 tcaccgttcg ggtaccgata tcgagcgcat atatcaatga aaacacctct cgcttttttt
     2521 caggcagcga cacctcgttc tctcattacg cggaaaatta taccatacat ttggtttgcc
     2581 ttagattttg tgctaagata cccatagtcc aaacgtgggg gttgaggttt ttgaaggacc
     2641 tacgattagt ctttgaccac ggcggattca aagtatacag agcgactctg ccgcccatag
     2701 gagtcaacac ctacttcctt accgatgata ccacgctcct cgttatcgat cccggaaccg
     2761 gcgctttcga cctggtccga aaaggcttcg agcagtttga ggaagcgacc ggtatcttaa
     2821 tcacgcatac ccattacgat cacatcgcgg gactacgcga gtttccccga aaacgtatct
     2881 ttctctccca agccgctaaa cccggtctgt tctcgccaga aaaaaacttg agtcagcaac
     2941 tctctaagcg aggattgagc ctttcacaag acgaagaaac ggttcttgtt ttatcagagg
     3001 ggcccacgga cgtgggttcg ttgcgattcg agacgtggat tctgccagga cacacccctg
     3061 gagacgccct cttcgacttc ggatgctgct tgttcaccgg agacatccta tttgaaaact
     3121 caatcggacg aacggacttt cccggagcca gcgagagagc gatgagccgc tctctcagaa
     3181 gactctatac ttacctgaaa acccaacagc cgaataagcc ggtatttccc gggcatatga
     3241 acgctttcac cgtacgcgat gccctcgaac gcaaccccta tttggccatt ttccgggaaa
     3301 tatccacata gccgacgttc gtgaggagga acctatggat tgtggcgtag ccggagtccc
     3361 cgaacgatta aaggcgatga ttttgaaaga aggaaaggtt ttacccaacg gcattttgaa
     3421 ggtcgattcc ttcgtcaatc accagatcta tccggcactg cacatggata tcggccgtta
     3481 cctctctgac cctttcttgg gtctagggat caacaaagtt ttaacgcttg agagctccgg
     3541 catcgcgccc gccttcgcaa ccgccctcac gctcggcgtt ccgatggtct tcgccaggaa
     3601 aaagaagccg atcacaatgc aggagtattg gcacgaatcc gcgccttccc acacaaaaag
     3661 cggtgtcgtt gacatttacg tttcgaaaga ggtcttatcg gaccgaaacg tcgtgttgat
     3721 cgtcgacgat tttttagcca ccggcttgtc tatattggcc ttagcccgca tcgtacaggc
     3781 ttcgggcgcc aaactcgctg gaatcgccac agtcatagag aaaaccttcg aaggcggacg
     3841 ggagttgctg atgaaatccc atcaaacaca cattcacagc gccgtaagaa taagtcgatt
     3901 aagcgaatcc gctatctctt tcgaatgagc tttccggcta gccgatcttt cagaactaaa
     3961 tcggcagata tttcagcttc ggaaaaggtt tgagcatccc tttgagcaca tttccgaaac
     4021 cgacctcgta aactccctct acgccgaacg cctccatctc cgacaaggtc tgcgaccacc
     4081 gaacctggcc ggtcaattgt cgggtgaggt tttccttaat ttgcccgata tcgttcgaat
     4141 agtttccggt gaagttagag aagaccggaa cccggggagt gaggaagacg gagcggtcga
     4201 tcgcctccgt cagctctttt tcggcgttct tcatataggg cgtatggaag gcggtgctca
     4261 cgttcaattc tacaacgatt tttgcaccgg cggttttggc cgaagccatc gcctcccgca
     4321 cctgatcgag agcgcccgaa atgaccgttt ggttcggggt gttgatgttg gcgatccaca
     4381 cctctaagaa ttgcgccacg atctgctcga cgtgttcaaa agggatgcca atgatcgcgg
     4441 ccattcctcc ctttttttcg gtagaccctg ccatcaataa cccgcgtttt ctcaccaacg
     4501 atagtccctc cgaaaaagaa aagacgcctg ccgcgtgcaa cgcgctgtac tctcctagac
     4561 tgtggccggc gacagcgtcg aaggtatgtc cttttgatcg cagaaattcg aaatacatag
     4621 ctgaaaccgt gtaaatagcc ggttgggcga tctcagttgg tttcaacgcc tccgggtcct
     4681 ccccaaagat tagggtttga agatcgaatc ccaaaatctc attcgcgttt tcgaggtagc
     4741 gtcgggaagg ctcgtgctca tcaaagactt tttttcccat ccccggtgcc tgagatccct
     4801 gtccgggaaa caacaacgcc gtcttcatct ctatctcctc cacaaataaa ggttacggag
     4861 cgcctttcct gcggatgccg aaagtgccct gttgaaagcg attcagcaga aacagcaaaa
     4921 cgctctctgt acgattttac cagttttacg cgctatttcg aaaccacaag gtgtttggta
     4981 tgaggactga gaaagtcgcc tgaatactaa aaaaacagaa agagtacgaa caaaacaaaa
     5041 aaaaccgtgt gggtcaagtt atgggccgta accgtgaaac aagaccagat tcgagcgcgc
     5101 gattataagt acacgtaatc atcgcggcgt aagcgcccct tcttcacgcg acggcgcgat
     5161 acgcatttgt acgggctcgt cgtggcgtag aaggggcaaa gggcggatcg cggggtagaa
     5221 aaactactta ccccgcgata acctcacaca ggcgtcgcgg cgtaagcgcc ccttcttcac
     5281 gcgacggcgc gatacgcatt tgtacggcca ttgcggcgta agcgtttaag ggctttcgag
     5341 agctttctga aaagcaaaaa cgtgttgaac taaagttcgg cgttcccaat ttgctgtgcc
     5401 ccgatattat acccactcaa aaaccccccc catatttgaa gaaatttggt acaatcagct
     5461 tataaaataa gaaaaaaggg agaggattat gcgtatcgtc gaagtaaaaa cgaatcggga
     5521 gagagcggct tttcgagatt ttccgtataa gctgtacaaa aaaaacgacc aatggtgtcc
     5581 ctacttcgtt cacgaaatga atcatatact ctaccacggc cggtctatcg tctacgaccg
     5641 gagtcaggca acgttcttcc tcgctatgcg tgataaggag gtagtaggcc ggatcagcgt
     5701 cgggatcgac gaggagttga acgctcacaa acgccaacaa cacgctttct tttctttttt
     5761 cgatgccatc gatgaccaag aggtagggca tgcgctgctc tcgaaagccg ttgagtgggc
     5821 aaaagagaag ggcatgttgt atcttaaagg atcggtttct ccgacgaacg gggacgattt
     5881 tcgagggata ctcaccgaag gatttcagca ttcttcctac tttttgatgc ctttcaattt
     5941 tgagtattac aaaaccttct tccgggagtg ggaccgctac ctggagtact acgccttttt
     6001 tcaagatatc aaccagccga tccctccgtc ccgttttaat ttgatgaaac ggattcttac
     6061 agaaaatctt tcagacaaag aagccgctct aataaccgat gaaaggcaat tgacccgcgc
     6121 ttttttagac ggttttttct ataaagaagg atacacgata gaagaggtca atttgaacga
     6181 cctctccaaa agcgcttgcg acctgtatga agtcttttct aaaagtattc ccgacgattg
     6241 ggaagaagac ctcatgccgt tcgactacgc ccaatgcgag caattggtca aagacttcaa
     6301 actgacccta ccgccgcctt tgtgtttggg cgtctactat aaaggaaaag cggtcggcat
     6361 ctccgccgtt cttccaaact ataacccgat cgtcagaaag atgaaaggaa ggctctttcc
     6421 cttcgggtgg atctctttgc tctcctccaa aaaccgcatc aaagaagggc gtgcggtgat
     6481 cctcttcgtg gttccggagc atcagaatcg gggcatttcc gctctactgg tgctcaagct
     6541 ccgagaaaac ttgaagcggc ttggttacgg gaagatagaa ttttcatcca tcagcgccta
     6601 taatacccag atgatcagat tagcagagtt catcggctgc aaacgctaca aaacttatac
     6661 agttttcggg aaatccctca tcgatcggcc cctaaccctc gaggaaacct acggtacagg
     6721 agccgaaacg atgcggaggt ttttcaaaca aaggccttaa tcgctgatgg cccgaaaaat
     6781 aagagaggcg ttatgccctc cgaacccgaa ggaatttttc agaaacaccg aactcttgcg
     6841 atagaccgtt ttttcgggga tgttcaggtc cgtgaaggct tcatctcttt gtgtcaaaga
     6901 gggcataccg tgaataaacc cattttgtgt ttctagtatg gcggcgataa gctcattcgc
     6961 gcccgctgcg cctaggccgt ggccgatcaa actcttggtg ccttgaacgg ggatgttttt
     7021 taaggcttct ccaaagctcg accttaagga gtttgcttcg gaaatgtccc cgatgatcgt
     7081 cgccgtagcg tgcgcattga taaaatctac ttgtgagggg ctcacgccgc tgacttcgaa
     7141 ggcttggttg atagcccgtg acgcgccttc cccttgggga tccgaggcgc taaagtcgaa
     7201 ggcgtctcca gataaaccaa atccctcgat ggtcccgtaa atccgtgcgc cgcgctgacg
     7261 cgcgtgttcc atttcttcca agacgagtac gcccgcccct tctcccatca caaatccgtc
     7321 gcggcgtcgg tcaaaaggat tggacgcctc tttaggattt tgattattcc gggataaggc
     7381 catcatattg ccgaacgcgc ctatgggcaa cggatggatt aaggcttccg atccgccggt
     7441 gaccaccagc ttcgtgtacc cgtgtttgat catcatagca gacatgatga ctgcgtgcag
     7501 cgaagtcgcg caagcgctaa cggtggcgaa gttgggtcct ttcaaaccga actccattga
     7561 cacggcgccg gacgccatat ccgggatgac catcggtatt aaaaacggag agatatactc
     7621 cggcccgagg tccccgaagt ctttcatcgc tttcagaagg gtctgtaatc ctccgattcc
     7681 ggacgaaatg gtgacgccgg cttgtttttt ccactcgcca tccatcgtta atgcagcgtc
     7741 ttccaaggcg aatcgggtgg ctgccacggc gaactgtatg aaacggtcgt actttttggc
     7801 ccttttcctg tccatataca ttttcgggtc gaagtcactc acctccgccg ctacttgaaa
     7861 agtataagga gaggcgtcgt aagcggagat tcgatcgatc ccgatcgtca tctcctccaa
     7921 tgctttggaa aaggacggta agtctttggc taacgggttg atcgtcccca tcccggtaac
     7981 cgctacctga aaagcttctt ttttcatgcg cacctccggt tccacaatat taaccgaccg
     8041 tattctcgaa gtatagcaaa aaaagaaaga aagtcaaaag atgacgggaa aactcggatc
     8101 gatgatataa tagggaaaca cccggacaga aacccggttg cgtaaaaagg ggtgagaaac
     8161 ctgaatatcg atgaaatcaa gtccgccata ccgcatcgat acccattttt gatggtggac
     8221 cgcgtgacac agttagagcc agataggata caagcgtata aaaacgtgac cgtcaatgaa
     8281 ccctactttc aaggccattt tccacattac ccgatcgtac cggccgtttt gcagctggag
     8341 ggcatcgcac aaacggccgc attgcttttg cacgatacct ataaagaacg agagggtatt
     8401 ccgctcttct tggggatcga tagcgctcgt tttttacgag aagtccgccc tggcgatcgg
     8461 atggactacg tcgttgaggt caagcagaag atgggagatt tttataaagt gaacggtcag
     8521 gttctcgtag aaggccaggt cgcggcgaga tgtaccttgt tcgttggcgt aaaattcttt
     8581 gatccaccgg aggcgcatgt ttgattcctt accgctctgt cgactattag gtataaagta
     8641 tcctctcatt ttaggcggaa tggcgtgggt cgggaaagcg gctttagccg cggccgtgtc
     8701 gaacgcgggt ggattgggaa cgatcggttc aggctcgatg accatcgaag tactacgatc
     8761 ggaaatcgac cgatgtcgag cgttgacggc aaagcctttc gctgtcaacc tccttatgtt
     8821 ggatcccgat gcggaagcga aagcggccct ccttttagaa ttaagggttc cagttgtggt
     8881 gttcggggct ggaaacccgg gagtcttcgt ggaggccttt cgtaggcgag aaatcgtcac
     8941 gatgggagtc gtttcatccg aaaacctggc tttgctgttg gaaagggccg gtgtggacgt
     9001 gattatcgga gagggttgcg aatccggtgg gcacatcgga tcggtgacga ccatggtttt
     9061 aatccccgct ttggcggacc tcgtgcgcac gccgatcgtc gctgcgggcg gcattgcgga
     9121 cggcagggga atcgcggccg cctttatgct gggggctcaa ggcgttcaga tcggcacccg
     9181 cttcattgcc tccgtagaat ccgacgcgaa cgatacctat aaacaattga ttctcaaagc
     9241 cggagtccgc gataccatcg tcacgggcga agatttggga caccccgtac gggtccttaa
     9301 gacaccgttt tctagaaaaa taaaaaaaat ggaacggcaa tcggccgatg aggtcgagag
     9361 tcttttgtta ggaagtttta gaaaggcgta tcagaatgga aacctaaacg aaggctcctt
     9421 tctcgccggc caatcagcgg gactagtaca cgatatttcg acgtgtcaag agatcatcga
     9481 gaagatgttc gcccaagcgg cgactcttct ggaatatact attcctcaca taaacgaaag
     9541 gagaaaacat gaacagaacg gaagtattta gcagagtaaa aaaagcgttg gtagaaaaat
     9601 tagatgtcga tgaagaccag atttctctcg acgcagacct aagggacgat ctggggggca
     9661 gctctctgga actcgtcgac ttggtcatgg acttagagag agaagccaaa atcaagatcg
     9721 aactcgaaga attgagaagt attcagaagg taaaagatgt cgtagaactg atccaaaaaa
     9781 aattgagcta attttgggcc cccgttttcc gtctctataa tcgggagaga aatctcccaa
     9841 aaagagaaac gccagatggg ggggttgatc ttgtactacg ttttttcgat gttcgcgtcc
     9901 aaacaagaag gttacctaga tgaaaaacgg acggtaaccg ctcaattcga agagtatcgt
     9961 gacgccaaaa acgcctatcg gcatttttca aaacagataa aagcgtggaa acgtttggat
    10021 cccagccata tagggcggtg taccctcatc gtcgcgccaa gtcaaagcga ccagtcctcg
    10081 gagtctacga aaccgagaga cctcctcggg cagcaatcca aactcttggc agaaagaaat
    10141 tagtcgagcc aaaaaaggga gcctcgctcc cttttttatt gtcggtcatt tcgtataaaa
    10201 tgaaaaaagc ggtcttagct ttttgtcttg aagaatcgat ataagaaata taaaccgatt
    10261 ccgatcaaga ccaacggaaa ggcgacggag aaaaaggttt gctgtaggag cgtgaagaac
    10321 ataaaaagag agcctatccc aaacggaatg actccgccga tgaacaaccc cttcttcgcg
    10381 ggcagcgtaa ggaaatacat ctgtagcaac ccgatcccag gcgccataac gaaaatgggc
    10441 cataggtact ggagtatccc gtaatccgtg taactgcaca ggaagaacag taaacccaaa
    10501 gtgatcagaa tccctccggg cactagtagg ccggagagtt ttttgttccg aaagaaagcg
    10561 atctcaaaaa tgagccctgg gagtaacaaa aaagacggcc atactctttc ccaggtaatc
    10621 gataggatcc caaaagcccc gagtaatccg aaaataccca aaattaccaa cgaaacgcca
    10681 aaaactaata tgagtgaacg tttcgcaggc tccgcgctat ttcgataggt tgttgatttt
    10741 tcttccattc ccatcttccc cttttgcccg ttttcttcat agcgtttttt cacaagccat
    10801 agccccttac ggatatacaa gtcagattat accactatca agagttctta tgccccctca
    10861 ctctctcttt cgtttgaaga accccgaccg tgcggttgca tttttctact gtttctgcta
    10921 cacttcaaag aaactgtttt caagaaggag aggaacccta tgtatgggta cgcgccgggt
    10981 agagccaata ttatcggaga gcacgtggat tacaatgacg gatatatctt ccccttcgca
    11041 atcgagcgaa aaacgacggt tcttattaaa aaatcggatg attcttcctt tcgcgttcga
    11101 tcgaaaggtt acggggcctt cgttataaat cctgagaaga tggagccgag aggggattgg
    11161 accgactata tccaaggggt tttttgggtg ctgaagagac acgatcgatt tcacctcttt
    11221 ccggtgaccc tcgaggtcga ctcggacgtg ccgatcggcg cggggctatc gagcagcgcg
    11281 tccgtggaag tggccaccct cgccgctctg aacgggttct tcacgctcgg gttagacaaa
    11341 gccaccctca tcgcctacgc caaagaggcg gaaaacgact tcgtgggtgt tcaatgtggg
    11401 attatggatc agttcgcatc agtgatgggt aaagaagatc acgcgatatt tatggacact
    11461 cgatccggtg atttcgagta tgtaccgatc gatttggacg ataagacctt tttggtgatc
    11521 aactcgaagg tctctcatgc cttaggctcc ggtgaataca atcgccgtag agccgaatgt
    11581 gcgctctgct tgaacacgct gcatcgtagc agcttcagag atatttcctt ggccgaactc
    11641 gaaaataaaa gagcgctcct ccctccggtg gcctatctca gaggattgca cgtgttaact
    11701 gagatagaaa ggacgtgtct gtgtaaaact gcgttactcg aaaaagattg ggcgttagtc
    11761 ggaagattgc tcacacaaag ccatagcagc cttcgcgata attaccaggt cagctgcgag
    11821 gagatagagt atctgatcga aaacctaaac caattctcat cggtgtgggg agccaggatt
    11881 atgggagggg gattcggagg gagcctcatc gccttggtgg aggagcgagc cgtccccaaa
    11941 atcctcgcgg agttgagcga taagtatttt caccgatatg gcatccgatt ggaagggtat
    12001 caagttaagc cctcaaaagg agcgggatac gttgacagtt agcgtagccg aaaagaggga
    12061 ggccctgttg ggcgagatcg gtaaaaccat cgaacgttct tcgcgcgtgg cgatcgcttt
    12121 ttccggagga atggactcca ctgtagccgc ctgctgcgtt cgagaggctc tcggtgaaag
    12181 gggcaacgcg gttttagttc acttttcttt cggcccttac acttacgaga agacggccga
    12241 aaacgtccgt ctcctcgcga agcggatcgg attcccgctg tatctggtcg acaagcggaa
    12301 ggagttagaa atgctatctc gaaaaggacc ctcttgtaac cggtgtacca agcatatcaa
    12361 gttagggggt atgcgggact tcgcgaaaga gtggcgggcg gattggatca tctccggcgc
    12421 caaccaaagc gacacgtggg gacagtatgg gatcgcggtt catcaaaaca cctattctcc
    12481 gctctttcac ctagaaaaac ccgaaatccg tgagctgctc gaccatttcg gctttgcact
    12541 ctcggaagtg cgttcgggcg aatccgctct gcgcgagggt tgcaaactga agcacctgat
    12601 gaaggcgatg gccgtacccg agtaccacgg agaagctgtt tgcctatcca atgaaacact
    12661 gttgagccgc ttgcgagaag cgcgttttga aacgcagttt gcaaacgtaa aaatcatcgg
    12721 ccctttgcgg aaaaacatcg cattgatcaa tgtctctcca cttccgccgg ctacgctgcg
    12781 cgaaaagctc gttcgcgaga taggagccct cgagtccatt tctgaggctg ccatcgtaga
    12841 tcgacccgtc acgctctatc tcaaagctaa ccccggaata atacgttcgc cgcattcccg
    12901 gcattggttg gaagttggga agatcggtcc agagttctca gggcctatcc gattcgtgtg
    12961 gatggagagc cccaatcggt cgctcaatac ctatcacgtc gtcgactata cgttcgctta
    13021 aggtttggac acaagcgctt gaaggaagtg aaaaggatgg attacacgaa aattcctccc
    13081 gaaacaaaga tcgtcgaccc ccggtgcgcc ccgttctttc acaacgggtc acgcgaatcg
    13141 atcctattgc tccacgggta cacggggaca ccctacgata tgcggtatct tgccgaacgc
    13201 ttatctcaac aggattatgc ggtcttcgtt ccccgattgc ccggacacgg aacgaaccgc
    13261 cgggactttt tgcaatcgaa ggacgacgac tggctgcggg aagcgatcga cgggtacctc
    13321 acgctacagg cgatgggtaa gccggttcac gtgtgcggct tgtcgatggg aggcgtgttg
    13381 gccttgctgc tggcggaaag gttccctata cggaaggtct gcctctgcgc gccggctatc
    13441 gtggttaaga acccaatgat gaaatggact ccctttttaa ggcacgtcgt gaaaacgacg
    13501 aaacgaccgc gggttgaata cgatgaagcc tttttaaaca cgctacattg tgagtattgg
    13561 agcgaccacc tcatcgaaaa actggccgat ctatcgcgtc ttcaaaaacg atgcttgaag
    13621 gccctagaga acgtgacttc tgactgtttg gtccttttgg caagaaaaga caccgtcgtt
    13681 ccgacaggcg tcgccgacct tctccgaaac cgcacgcggg ccaactcctt ccggacggag
    13741 atcctggaag aaagcggaca cgtcatcctt aacgatagtg aaaaagagaa ggccgcggat
    13801 accataatcc gttggcttga taaccgctag actgacaaac agcgcctgga acgagttcac
    13861 ggagatattg actttttcta ttccgttgga ttatactacc gcgtagtctc gctaagcgat
    13921 cgcgtctacc gggaggataa gcgttattgg ctggttacca actgttttgg ctatgcaaac
    13981 tgtgtcgagg ggattgttgc cgcatctatg accctacgcg actcccaaaa gggaagagta
    14041 tcggcgaatg gagcgacgag ttccatcaat tcagagaggg atacggagtc cttccgttgt
    14101 ttatccccga aatcgtgcat catagaacaa acgcccatct gttggaaatc ttacgtgaaa
    14161 aagggatcga ccccttcgct tgcgaatact tgggcctcga gggatgtctg atcgcctggg
    14221 ataatcgtcc aggccagtgc atcgcctatc gttgccccac ttttaaagat tacgataagg
    14281 acgcgagcta agccgatgat acgacgagtt tgcagcatct atccacaatc cgtgaccccc
    14341 tcgcccttga gtcacctgtt cgcctacttc cgcttcgtcg ggtaccgctt cgcgagaggt
    14401 tgcattcagg tcttggacgg tttactgagc atactcctcg cgccgctcgg tatggatacc
    14461 gaaatttacg cccgcttcat ctaccgggtc gaaaaatacc ggaaacctct gtggaaccga
    14521 cggtatcaaa acccgaactc tctttgagct ctctctgcat cggttatttt cgccctccta
    14581 cgtttaacgc gtatccgctc ggcgacgaag tagccaaacc cgcccttctc tgtcgaaagt
    14641 aaggaatccg acttcctacc gagtgataag ccgattaagg agaacagagg gattttatag
    14701 aattgtataa aggaaaagac acctacggga ggcatgcgaa tgagaaaaac gctggggatc
    14761 atcctttgtt tgttactctc cttaatggct tttggagata tcatttattt agttgacggc
    14821 tcgaaactat caggaaagat actgggctac ctgggcgacg aagtggaagt attgttggaa
    14881 acgggacatt ctgtcaagct tagaacgaac caaatcatcc gtattgagcc tctcgatacc
    14941 ggtcttttag cggcctccaa cgccgcgata gaaacggctc ctttggccgc agcccccgca
    15001 gccgccgcag ctgccgcccc cgccaccccc attcccgcgg gccactggcc ataccaaggg
    15061 tattcggtga ctggaattcc ggaagcgacc ggatacatcc cggctaccga aatccctgtg
    15121 atgttcggcg acccggaaac ggagatgcga aagaggttat taatgtatag tgagatgaaa
    15181 aaggagccct gggtcgcggc ggagctttct ttgctcattc caagcgccgg ccatttgtac
    15241 accggcgagt gggaaagagg attcctattc ctaggcgcga aaaccgcttt cctcggtgtc
    15301 gctatctttg ggtttttacc gcaggaaacc ggtgaagtgg atagcaatga taagcctatc
    15361 atgaagcccc agaatacttt gctgggtgca gtcggtgcgg ggggattcgc gcttttcacc
    15421 cttctcgagt tcgtagactc ttattatgcg gccgaacggt ataatcaagt gctacgcctt
    15481 cgtttgggca tcgaacggtt cgatccgatg atgttgccta cgttcagcaa ataaagaaaa
    15541 gcgcatcgcg cgcttttctt cgctctcttt gagtataatt ctctgagtag ccagctcaaa
    15601 ccaggattgc cgatggcgca cgaaacgacc tgcttatggc tgcttccttc cggacctgac
    15661 ccggttcaca gtgttccgtc gcatcgggcc caattctcaa caccgcttgc ccagtatcct
    15721 tatacccaat tcaacctcgg tcaagcatca gccccgctat agtggatttc gggttcagga
    15781 ggccactact ctcccgcctg cgcgatacgc cggaagtatt ttaccataat tccttccgtt
    15841 tttctacccc ccgtgtgggt caagttttgg ggtgtaatct tgagacaaga caagaatcga
    15901 tcgcacggtt tttagtcggt gtggtcatcg cggcgtaagc gccccttctt ttcgcgacgg
    15961 cgcgatacgc tttgtacctg tcttgaaaaa cagggttcgc acagaggaca cagagaaaaa
    16021 atcttgggaa ctccttgggc acgccaactt ttaagttggc ttgtctttga aaagaaaggc
    16081 gcaaagcgta tcgcgagact tcgtgggctt ccgaagtcag cccgctggtc accctgcaat
    16141 gaacctctta gtattgtcac ctcataactt gacacgcgct catctcccgc tatctcaaat
    16201 cgcggagtgg atagattaaa accacaaatc gtctggtagt caaaaaaaac gtcgctgacc
    16261 ggaaaagtct gatggtacga acgagttgat tctcacttcc ctgaagccca cccgccccga
    16321 aacttggtcc tttcttacgc tagcaaaatc agtagaacta tcccaatatt gattttttct
    16381 ccactttagc ggtttactca ttatccaaat ttttaaaagg ctcggaaaag ggcagccacc
    16441 gcaagaaaag ctaagctcaa catcggtttt tcgggtgtaa aaccgtctcc gcacgatgaa
    16501 tctggcgctt tttaacgttc agcggaccac ggataacaag agtcgattca gatagttttc
    16561 caaccactcc tgccttccgg aagcgggcaa cggtataggg gtgtcgatga tcgcactttc
    16621 caaagaatgg atatcgacag ggcccttttc gatctctgcg ccgagaccgc tatcaaaaga
    16681 gacgtagcgc tcttgtatcc ctctctccag gaacggatgc tcaatcatca ttttggccgc
    16741 tttcaagccg atggcgaagg cgtccatcgc agcaatgtgc gatagaaaca ggtctttctt
    16801 ttcataggag ccgcgccgta cgtgagcgtc aaagttcagc cctccaggcg ccaacccgcc
    16861 gttgagaaga atttcataca tcgcgagcat ggtatcgtac atgttcgttg ggaagtgatc
    16921 ggtatcccaa ccgaggagca ggtcgcccat attcgcgtcg acactcccga gcttgccgac
    16981 catcctagaa taccgcagct cgtgctgaaa ggtgtgaccg gccaaagtcg cgtggttggc
    17041 ttctatattg aacttaaaat acggttccaa cccgtaacgc tgcaaaaaag ccaaagaatg
    17101 agccgcgtca aagtcgtatt ggtgtttcgt cggctccttt ggtttcggtt caatcaaaaa
    17161 ttgaccttca aaccttatct cccgcgcgta atcgagaata atccgataga aagcggccag
    17221 gtggtcgagt tctcttttca tatcggtgtt cagaagggtt tcgtagccct ctcgaccacc
    17281 ccaaaacaca tagccttctc ctcccaacgc tagtgtgacc tccaacgctt tcttcacttg
    17341 ccccgcagca taagcgaaaa ctttcgcgtc tggagacgta gccgcgccgt gtagatactt
    17401 cgggctggaa aataaattcg cggtgcccca taagagtttt attccggttt ctttcatgag
    17461 tgcctggata tgcggaatca ctcggtccat caatgcattg gtctgcctaa gggtgtccaa
    17521 ttccggagcg agatcgcgat cgtgggcaca gaaataagag acacccaatt tggacataaa
    17581 ttcgaaagca gcgtccgcct tcgcaatcga ccggtcataa ggatcggcat agcgattcca
    17641 agagcgctcg aaaaacggtt caccaaacat atcccgtcct tcgccgcaaa aggtgtgcca
    17701 ataggcaacc gcgaaccgga gttgctcgcc cgctttcttt ccgccaatta actcctccgg
    17761 attgtagtac cggaaagcca gcggattatc ggaatcggga ccttcaaatc cgattttctc
    17821 tacattcgga aagtagtctt tcattttaac cctcccttta tattgcttgc gacaatcaat
    17881 caaatagaac aaatacgctg tttcatagct cttccaaata gagaggacaa tcagtggcgc
    17941 catccttgag gagagccttt tccgcgaagc ctacggctta taacgaaaac acgcgtcttc
    18001 gaaaaaagtc gggctcttgt caaattctga gtgtcttcct caagcaaact tcgattcacc
    18061 agaaccttga gcaccaacag gtaggaaaca gggcaagtag actcgccgat gccgagtggt
    18121 ttgcgtcctg tctctcagcc cttttggaca acaggcggat ttttggaaga atcggcgcga
    18181 tatgattctt accggcaaaa atccgctatt accggcaaga gcccttacag gagtcgatgt
    18241 gttatgacta tggctttcct acgacgtctt ttatcctaaa gagctcctta acctcctgtc
    18301 cagttcgagg aattcgttga tttctttaga aaaagcgatc agcaaatacc accaatatat
    18361 gccgagagtg atgatgatga acagaacggt taaaaatacg ttgcggttct tcattttccg
    18421 aacgggtgtc gccatcgcat tttggtagac gctataaaaa cggtctttgt actcttgtaa
    18481 aagggcagcc gttttgaaag tctgttggag atagatgccc aagaacacga acccgatgat
    18541 gttgaacagc caagaatagg cggggaccac gatacctaaa aagtagagtc caaggtaaac
    18601 ccaaaatgcc cagggttcaa atttcagtgt atccagcctg tactcaagct ctcggtaatc
    18661 actattctga ggattcgccg ctgtcaatcg tcggcatata agccgcgtgt tatcgatgtt
    18721 catattgaga gaggttgacc attggaagat gactatcgtt tgtagaatta aaacgatcac
    18781 gttcgtcagg aagctgaggg taaatatggc gcttccggcg aacaagcctc cgatatctcc
    18841 agcatagacg ctctgcacag aagtaaccaa cccaatgaga gaaaacacta cgcccaaaat
    18901 accgatgatc agtgacgcaa tagccaaatt cacgttgacg cgtttttcta aagtgaagtc
    18961 gacgagtctt ccggtcgccg gcgtagccgg tgaagtcctt tgtgtctcga ccctttcagg
    19021 cgcgcctatc cgcgccccgc agaggcgcaa aacttcgagt catcggggtt ttgtttccca
    19081 cattgactac agtacatctc aacacctccc agtcaaacga aacgcgagcc cgctcactcc
    19141 tggtgagtcg ggtctttcga tacttccggc gcgggttcct ccggttccaa cgtatcctcc
    19201 gaaaaggcgg agggttcttc gacca
//
LOCUS       DAOPXN010000023        19021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_2936_length_19021_cov_6.034272, whole genome shotgun sequence.
ACCESSION   DAOPXN010000023 DAOPXN010000000
VERSION     DAOPXN010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 19021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19021
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2936_length_19021_cov_6.034272"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PKM49_02400"
     CDS             complement(<1..257)
                     /locus_tag="PKM49_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY81699.1"
                     /translation="MKESNRVIETNVGIQDGEIVEQFDQMMRAMAGERRRTIEKMKSL
                     GIDGGCALEIGPGPGLLGIDWLKASPGARLKALEISANMAR"
     gene            complement(341..880)
                     /locus_tag="PKM49_02405"
     CDS             complement(341..880)
                     /locus_tag="PKM49_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNY81700.1"
                     /translation="MNVRLVILGLLQRRPMHGYEIKQEIERGMGDWTDIAFGSIYFAI
                     GKLAEEGSISESGQERVGNRPSRTVFTITEKGRQEFLRLLREEWQTERRQYFPVDLAV
                     YYMASLPKEEVHDYLVELCRKSEESLRTLESHAEALKVVPYIPPLAFVIVDHTRRHLL
                     AERDWLESIIASLNEIYGA"
     gene            2460..4502
                     /locus_tag="PKM49_02410"
     CDS             2460..4502
                     /locus_tag="PKM49_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81701.1"
                     /translation="MKRGILSCLVGFLSFVFLLSCGGGIPKLPDGILDISVNEIFDAY
                     VDIAGLATPSLYSAPETPIELSPFSPVLTITLEKSEGTVFHILFLGKAYDLEFGTLNI
                     DLTEAESGLYPLTVTTRAGVSSRDSQKQVWIKVDNGIEMPKVTLTWLEKELATIELFW
                     KTDSAENAFVSLYLRSDTAGEAIDFGIEELQPNVGRPVTFSNLQKGQSYQVGLKRNGE
                     PTINWLASFKTFRDMEEGLQLFYWLDLTEPSTHKVKVGVFGYYKDLSELYLDNYGYHT
                     EGGLARAQERELYSEPEISMDIQENNKLLQFDLKNNPEGYFRLSYTSDKNAIVTSNHG
                     IQGYFCPDYLIASHEQILYGPGAFIGRSGILNENPDFFRASLSLYMLGGWDASTGWEE
                     ESPGVYTFPATYSSKIEEFFHASHIYAYQSDAFIKETKQYGAHSFHIILSGSVDRRII
                     NDLQTIYQFLTALWGGTMDIPAYTIMITDPPNNDQSLYAYAGEWSTGQGFSSNWGIIG
                     EMVIHQMFHLWNAWAKGIPCTHDYHMGFWYEGVNEYYCDKILTEIDFQTSSFPPHPCL
                     EEWYRIYKTVRGTSADKPLIIKDEGDPFPDKTLVYWKGAVFAYALDQEIRNRSSGRYS
                     LDDFLKHLLIQYNERGGLFSYAAILDYLRQIIPAGLDDWWQQYMVNNEPLFLEEFE"
     gene            5301..6992
                     /locus_tag="PKM49_02415"
     CDS             5301..6992
                     /locus_tag="PKM49_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81702.1"
                     /translation="MFKKVMAVSCLVIVISLFGFASSLSTNAPPDVQLSFHVTAEFWK
                     DHIANHGMLIEIHFSQNGGYEAIKKISLLGEFGRGKGEIVLKGENASAELLLWDPTTR
                     FLEYFIHLDDDFELKTGSRYTLKFLTTNGLIQSYDFIIEDAERDYPNITSPTLNAHFS
                     TPEEIKVRWTHPEVSRPNDTIEGKVIESYLFLLIKRKTEFGSEGEMSRILFPVPSNRY
                     EVTIPRATLLNNSMYRIYVEYTAHFRENPIYSKKVRNYIDFVIGDYQYTRMLNETQGQ
                     PKLNKANPEAGYHFPFYTYFNENADTNQPVHIMVLPLITPRPSDDFNYFLRSAEETIL
                     GGYSRFNGLNIMFLCPVFPRPEIDAYVPCLDHKTLKLTNPPELIRIDLQLIEMVKIFR
                     KQLEDKGIEVAQKLLFEGYSGSAAFSIRFAYIHPELVEAFSAGHCAGWPVLPIAVYEG
                     KTVPFPLGIGDLYKLTGKEFNIRALKEVKVFLYAGTADDNFHFDNDYLDLAVKILGSE
                     AKDIWPNALRKYYENGLYNVTIRLYSGLNHSTTYPAAIPDVVRFFQSVLTNSPQK"
     gene            7898..8362
                     /locus_tag="PKM49_02420"
     CDS             7898..8362
                     /locus_tag="PKM49_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNY81703.1"
                     /translation="MQITVVNRALSLFSEGYSCSQSVALAFLENSLPPSVVAALSASF
                     GGGMGRSGQTCGAISGAVMVIGYYYGQRVPFEAEKKERAYGLVQQWVKAFNAEFGHTT
                     CNGLIGVDVADENQRARAREQGLFDARCPRFVQRAAELTHQLLIKHPIAYPR"
     gene            8853..9914
                     /locus_tag="PKM49_02425"
     CDS             8853..9914
                     /locus_tag="PKM49_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY81704.1"
                     /translation="MKKSVFLLLSLCLFTALIAAELPLKIGFLYVGPVGDEGWSYAHD
                     LGRLSLEARFGDRVETVYRESVPEGAETGATVEELILEGCKVIFGTSFGFSDALYDYS
                     LRYPEVHFFQCSGIKAGHNLSTYFGRIYEPSFLAGLAAGSMTRSGKIGYVAPVETPEV
                     IRIANGFALGVRAINPEARVYLRWTGSWYDPDLEESAAAALINRKCDVIAQQTDSSAP
                     AMLSEQRDVWAIGYNSDMSQAAPTKMLTSSVWDWGIYYTETIERVLDGTYRHVAFWEG
                     MDSGLVGLTPLSDRIPEETRKLVQDYERRMLSGDFHPFRGPLYDQGNQLRIEAGRVMT
                     DEELLSMSWFVDNVVTSAP"
     gene            complement(9959..11257)
                     /locus_tag="PKM49_02430"
     CDS             complement(9959..11257)
                     /locus_tag="PKM49_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNY81705.1"
                     /translation="MSFKMAKDLVIEFQAREVTRLSRQYMDLLDSPHPSVRLQGINGL
                     KKTGTWTEYALKLAEDSNFGVRKALAEYIKETGTQDQQPVIEQLLMDPDDRVRLSALN
                     ALYAINPEPERLRVLLQDPSPKIRVRVLKLAASELPPEDVQPLLEDRHVSVRAEAYQA
                     FIERTEDPVALEKIAQETPFPQARKSALLKLLVFRPDFVQKFFSEMLSSDRYTEQEKK
                     VALTTLKHLPYDAIASILRQAFDDPRLRAHYPVLIPLYVEINLDSPATAISFLTAWLE
                     AEEPKIRLSAVKGFGKLAEPATVTLLRERLQDSDETVRAAAVDALARLMDYELEKVVG
                     DLLQDHSYLVRKAAIKAISRLKLVDEYANFVERMNEKKEETRLRVACIEHSAKARYGF
                     AIESLKRIMGDLSEASLIRSAAAYALLRLSPAAVIELLSQ"
     gene            complement(11269..12390)
                     /locus_tag="PKM49_02435"
     CDS             complement(11269..12390)
                     /locus_tag="PKM49_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNY81706.1"
                     /translation="MGKQEGTRSRVVNFKGVLYFLSILLVFGLIILYSASYIMVYRWP
                     GIDLAYYFTRQVLNISIGLVFMFVIAMIPIEKHLQWAPGYLVGLILLLVAVFFFPSKG
                     GSHRWIDLGFYDLQPSELAKFVIILFLAAYFDKRQFQENWKVDFFDKFLIPVGWILFI
                     SYLIFREPDLSTSMIILSIGFLLMFVAGLRYYYFFLLAGLAVVGLVAVLQFELLKPYQ
                     LDRLRGFLDFFTGSAVDTQVQIGIDAISSGGVLGGGLALGDYKYLLPVQFTDFIFAIL
                     GEELGFVGMAFLLILYYLMARSLIHAAIKGVNRPAGKLIVVGYAYLVLIQVIINVGVV
                     LGVFPPTGIPLPFISYGGSSMLAYLGGFGLVISAITHDE"
     gene            complement(12354..12818)
                     /locus_tag="PKM49_02440"
     CDS             complement(12354..12818)
                     /locus_tag="PKM49_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF123 domain-containing protein"
                     /protein_id="HNY81707.1"
                     /translation="MANNTGSYLLLIELNKGREIHYGSLKQHLRRGYYVYVGSAMNHL
                     RQRVHRHIDYKDRFAENPKGKRVEHWHIDRLLVYGNIKMALLLPNTNKIEEEISEMVS
                     RFFKAVPSFGATDCVKVDSNLYCLRTVEDFHFITRKALELNWENKRGLEVES"
     gene            complement(12808..16095)
                     /gene="glyS"
                     /locus_tag="PKM49_02445"
     CDS             complement(12808..16095)
                     /gene="glyS"
                     /locus_tag="PKM49_02445"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNY81708.1"
                     /translation="MGFWLVLALFIDQASKLWVEHHVMVGQQLPVIPEVFKITHVKNS
                     GIAFGLFQGHGDSLRLIVLAIAIFVFFIGLRYARKSYLLGFAFGSIVGGALGNLLDRF
                     LRDGWVIDFLSFWNFAIFNVSDSFIVFGALGLGFYTIFYEKKVAKPMLEMEKSPENVA
                     LELRQYLERNVSWSTDLPRFAALLTGWFALQKIPYQLHLYDASLPEVSRGSPVTNRTP
                     GTNAFVDIIPATDSEPEENTRFLEKVAVAPFSEGVEGEILFVVKKDKRNRKKISDRPL
                     KFSLMGFSELKPMTENPDYLPISMMLNSSDIPLALTKEEKIVGKLDAFRIIISRAENQ
                     ERASSIASPQRQRDTLLPCSEVVTRARSCFQEDTETGGIRGMGLTFTLEVLTEELPPT
                     EIPGLLSNLEEKFRQCFIDFGLRPTGMESFVASRRFGIMVSGLEPRQPDREERKKGPA
                     CKVAFADESRLEPTKALNGFLASNGAKVDEIEIEEINGVPYVFIRRIIPGKMTVDLLA
                     SAVPTILSGLSFHRPMRWGAGETSFVRPVHGLLALLGSEIVPFSCFGVTSGRTTRGHR
                     FLGAETTIDRAEDYFDKMRHQYVFARQDEREGFIRQQLTDLAKTCSVEIPVDEALLKE
                     VTWLTEYPQAVMGQFRSDYKTLPKEVLITTLKHHQRTFPVLRDGLLTGGFVSFQDNGD
                     EAKANVVKGYEKVINARLADARFFFEEDQKTPLRDRLNHLKNVLFQKGLGSTFDKVER
                     NRDLAAYLCESLGFPAPQRALMDEAARLSKSDLLTSVVYEFPELQGTMGRVYADLEGL
                     DPAISKALEEQYSQSVPESVAGAVLSVADKMDVLCGNLWLGNVPSGSKDPYGLRRKLY
                     QIIETYISYNWDLRLDDLFRQGLKPFVSDRPSGTAPSTARPERSEDALLQTFKELTQS
                     RLEFYLQGKGLPYDIIRAVQHQWATPMRAAIVAQNLQEFRTEPVFLQISTLFERVHNI
                     TRNVTDTDFDSRLFENEAEKELYIRYTETKDTVLSHIRRLNYRDALIGLMELKNPIDR
                     YFDDVFVMVDREDLRLTRLRFLKSIDQLFMAAADLSMIEKDGGAQPDSVVSEKQNPEE
                     RGIPNGE"
     gene            complement(16101..17216)
                     /gene="prfB"
                     /locus_tag="PKM49_02450"
     CDS             complement(16101..17216)
                     /gene="prfB"
                     /locus_tag="PKM49_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNY81709.1"
                     /translation="MIPYESKSEFEENLRKFGELSTLFDLHGAQARVGQLEEEMANPD
                     FWTETTRSAQVLKEVKSLKAQIGEFSSLKSLIEDAQAALELSAEDDSMEEQALELFRK
                     VARGVKEYELAMLLDDEFDANNAFLTIHPGAGGTESQDWAQMLMRMYLRWAERKGFTT
                     RIIELQPGEEAGIKEVTLLIEGPYVYGHIRREQGIHRLVRLSPFNANNLRQTSFASVS
                     VLPELDDSIVVDIDPEDLRIDLYRASGAGGQYVNRTESAVRITHLPSGIVVACQNERN
                     QHQNKDTAMKILAAKLYQLEEEKQKKEISNIQGELNRISWGNQIRSYVFQPYKMVKDH
                     RTEAETGNVAAVMDGDLDAFIEAELIFYSRKNVRAQK"
     gene            complement(17222..>19021)
                     /locus_tag="PKM49_02455"
     CDS             complement(17222..>19021)
                     /locus_tag="PKM49_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNY81710.1"
                     /translation="TKGTSQIYNVFARMATGYKKDVDFTKDEKSRTVTLTDEGVKKTE
                     KNLKIENLYDPGNIDQLYHILNALNAIELYKKDVDYIVNEEGEVIIVDEFTGRLMPGR
                     RFSGGLHQALEAKERLRVQEESLTYATITFQNFFRMYEKLAGMTGTAKTEEEEFEQIY
                     VTDVVVIPTNMPIVRKDHDDEIYRSQKEKYGAILKEIAERHKKGQPVLVGTTSIEKNE
                     FLSRELTRLGLPHQVLNAKYHEKEAEIIALAGEKNAITIATNMAGRGTDIKLGEGVVE
                     LGGLCVLGTERHEARRIDNQLRGRCGRQGDPGSSKFFLAVDDDLVRIFGGERIGKIMD
                     FVKIEEGEPIAHPMLNSLIEQAQKRVEGLHFSTRKYLLELDTVMDSQRKEIYEHRDWI
                     LSKEDLSEHMKEIYRDIVDRRISQYCDTNDWDLEGLLQNLKLFPIDFDEIKSGAFNRK
                     EEIAERLLAKFMEAYQAKRDEIGADFVGLQKYLLLRVVDERWRKHLESIEGLKEGISL
                     RAYGQKDPVLEFKRESFILFEEMTDAIYDDMANLLLRVRVTTKYEEETRKSQKEIDDL
                     SYRHDEFDSFNRKLRRKVEDKASAAHRRMKVKR"
BASE COUNT         4618 a         4815 c         4696 g         4892 t
ORIGIN      
        1 ttacgggcca tattcgcgct gatctccaac gccttcaatc tcgcgccagg cgacgctttc
       61 agccaatcga tccccaaaag tcccggacca ggtccgattt ccagcgcaca accgccgtcg
      121 atgcctagac tcttcatttt ttctatggtc cgccgcctct caccggccat cgcgcgcatc
      181 atttgatcga actgctcgac gatctcgccg tcttgtatcc caacattcgt ctcgattact
      241 cgattacttt ctttcatttg gatcctccca ttcgataaga tataatattt aatcttgtat
      301 ataaggataa aaaaataata tgctgaaaat cccgtgaccg ttaggcgccg taaatctcat
      361 tgagagaggc gatgatcgat tccaaccagt ctcgttcggc gagaaggtgg cggcgggtat
      421 gatcgacgat gacgaaagcc agtgggggaa tatagggaac gactttcaaa gcttcggcgt
      481 gtgattccaa cgttctcaag ctctcttcgg attttcggca gagttcgacg aggtagtcgt
      541 ggacctcttc cttgggcagg gaggccatat agtacacggc caagtccacc ggaaagtatt
      601 gtcttctctc ggtctgccat tcttctcgca gtaaccggag gaactcttgc cgtccttttt
      661 cggtaatcgt gaagaccgtt cgagaagggc gattccccac tctctcctgc ccggactcgg
      721 aaatcgaccc ttcctcggcc aacttgccga tcgcgaaata gatcgaccca aaggcgatat
      781 cggtccagtc tcccataccg cgttcaatct cttgcttgat ttcgtagcca tgcatcggcc
      841 gccgttgcaa caacccgaga atgaccaaac ggacgttcat cctaatcctc cttcttatat
      901 atactacctt gtatatacta acacatataa tgatttttgt caagagtagt ataagaaggg
      961 tgtcacgccg cgggtgcctt gctgggaacg cctgagaacg tctgggaacg tcttgggaac
     1021 gccgcacttt agtgcggcat gtctttgact ctcgcttttt cggcttttcg ggaaagcctt
     1081 gggaatgcca acttttaagt tggcatgtct taaaaataca gggttcgcac agagggcaca
     1141 gagtacacgg agaaaagatc cttgagagcg acttgggaat gccttgggaa cgccgcactt
     1201 ttgcgcggca tgtcttaaaa aaaaacaggg ttcgcacaga gggcacagag tacacggaga
     1261 aaagatcctt gagagcgcct tgagaatgcc ttgggaacgc caacttttaa gttggcatgt
     1321 cttaaaaata cagggttcgc acagagggca cagagtacac ggagaaaaga tccttgagag
     1381 cgacttggga atgccttggg aacgctctgg gaatgccaac ttttaagttg gcatgtctta
     1441 aaaaaacagg gttcgcacgg agaccacgga gtcacaaaga agagaggtgt ttaggtgagc
     1501 caacaccagg tttatgtttc ctctgtgcac tctgcgtact ctgtgcgaaa ccatcttttg
     1561 cgtttttgaa cacgccgaac taaagttcgg cgttcccagc cgcgccatcg cggcgtaagc
     1621 gcttcttctt ctcgcgacgg cgcgatacgc ttgggaacgc caacttttaa gttggcatgt
     1681 cttaaaaaaa acagggttcg cacggagacc acggaggtca caaagaagag aggtgtttag
     1741 gggagtcaac accaggttta tgtttcctct gtgctctcta cgtactctgt gcgacaccat
     1801 cttttgcgcc cctgatagaa tcttgcaaga agccgttcga ttcgggcttc atcggtcgat
     1861 tcacgtgcaa catcattctg tgtgccaaca aacaaatgtt gaaagtgaga gaattgatgt
     1921 ccagtcgcct tcgctacgga tctagtttac tgcgaatata ttagtttatt gttgatttag
     1981 ttaagtaatt taattgaaaa tttacaatca aaccccttcc cgccgacttt atgctataat
     2041 aatttgattc tcttttcaac tatctaaaga agcaagaacg ttgcaaaatg atactgtgcc
     2101 cgatatggtc acagcgaaat agtcccgctt aaaaacattg agaaccgggg aatgaggaga
     2161 agaagttttc agatttcccg atcgattgct ttggaggggc tttagttttc agattttaag
     2221 aaaaccaatc gtaaggaacc gaagatccgg agtctttgcg atttctgaca aatagtcccg
     2281 tttcccagac aggaagtttt ttcttgcctc ggagacgttt ttaaccgatg aaatcggtta
     2341 tatacccgga accatacgag aaaatgcctg cccgagctat catagagcct aaatgaataa
     2401 gttttgatta tgactcacct attcgaaaag ctcgtttttt gcataaaggg ggttataaaa
     2461 tgaaaagggg tattctatct tgtttggttg gttttcttag ctttgttttt ctcctttcgt
     2521 gcggaggagg aattcctaaa ttaccggacg gcatcctcga tatttccgta aatgagattt
     2581 tcgacgccta cgtggacatt gccggcttag cgacgccatc actttattct gctcccgaaa
     2641 ctccgataga gctctcacca ttttcgcctg tcttaaccat aacgctcgaa aagtccgaag
     2701 ggactgtctt tcacattctc tttttgggca aagcatacga cctggaattc ggtacattga
     2761 atatcgattt aaccgaggcg gaatccgggc tttatccttt aacggtgacg accagagccg
     2821 gagtttctag ccgcgatagc caaaaacagg tttggataaa agtagacaac ggtatcgaga
     2881 tgcccaaggt aaccctaacg tggttggaaa aagaattagc gacgatcgaa ctcttctgga
     2941 agacggatag cgcagaaaat gcgttcgtct ctctttacct tcgttcggat accgcggggg
     3001 aagccattga cttcggtatc gaggagcttc agcccaatgt agggcgcccc gtaaccttct
     3061 cgaacttaca aaaggggcaa agttatcagg taggcttgaa gcgaaatgga gaaccaacga
     3121 taaattggtt ggcatcgttt aaaaccttcc gcgatatgga agagggatta cagttgtttt
     3181 actggcttga cctcacggaa ccgtcgacgc ataaggtaaa agtaggagta ttcggctatt
     3241 ataaggatct atcggagcta tatttagata attatgggta tcatactgaa gggggcttag
     3301 ccagagccca agagagagag ctctattctg aaccggaaat atctatggat attcaggaaa
     3361 ataacaagct gctccaattc gacttaaaaa acaaccccga agggtatttt cgcttgtcct
     3421 atacttcaga taaaaacgcg atcgttactt caaatcacgg aattcaaggc tatttttgcc
     3481 cggattacct gatcgcctct catgagcaaa tcctttacgg acccggcgcc ttcatcgggc
     3541 ggtcgggtat tctcaatgag aaccccgatt tctttcgcgc cagtttaagt ctttatatgc
     3601 taggcggttg ggatgcttca acgggatggg aagaagaatc tccaggggtt tacacgttcc
     3661 cggccactta ttcgtcaaaa atcgaagagt tctttcacgc tagccatatc tacgcctacc
     3721 aatctgatgc ctttatcaaa gaaacgaagc agtatggcgc ccattcgttc catatcatat
     3781 tgtcaggatc agttgataga cgaatcatca atgacctgca aaccatatat caatttctca
     3841 cggcgctttg gggcggcacg atggatatac cagcgtacac gataatgatc accgacccac
     3901 ctaataatga ccaatccttg tacgcctatg cgggagaatg gtcgaccggc caggggtttt
     3961 cgtccaattg gggaatcatc ggtgaaatgg tcatacacca aatgttccac ttgtggaatg
     4021 cgtgggcgaa aggcattccc tgcacacacg actatcatat gggtttttgg tatgaagggg
     4081 tcaatgaata ctattgcgat aaaatcctaa cggaaatcga ttttcaaact tcttctttcc
     4141 ctcctcatcc ttgcttggaa gaatggtata gaatatataa aactgtccgg ggaacgagcg
     4201 cggataaacc gttgataatc aaggatgaag gggatccttt tcctgacaaa actttggtct
     4261 actggaaagg cgccgtattt gcttacgcgc ttgatcaaga gatacgtaat cgatcgagtg
     4321 gcaggtatag tctcgatgat tttctgaaac acctgttgat acagtacaac gaaagagggg
     4381 gtttgttttc ttatgcagca atcttagatt acctccggca aatcatccct gcgggcttgg
     4441 atgattggtg gcaacaatat atggtaaaca atgagccgct gtttctggaa gaattcgagt
     4501 gaaatcgagg ggctgaatct actttttctc tttccggccc gattagatga tagtcagatc
     4561 agattccacc aaagtcgtaa agaggaaaat gcagcaatat caaggagcga tggattcgaa
     4621 tctgttccgt aaggtttaca ttatcgaggg gtcttagcca acttgacgag agcgatttgc
     4681 tgatcgtcat atcctttcca gataaatgga gaagacgtaa aaaagtttcg gagggttaac
     4741 caaacactga gtaagaattt ggctgattcc gtcgcatcct gatggtcatc aagatcaacc
     4801 gaaacgaaga caaggagtct ctcccaagaa aaatgttatc aggtagggct cgttgagttg
     4861 gaaagacggg tgaagtaata cctagacgcc tacggggaca aataaactga atccgcgaag
     4921 tatctggcga aagacgaaga ggaggggctg ttctttttcg ataatacggc ttcggctttt
     4981 cggcgcatcg gaatcaccaa tctggaaaaa tcgaactttt tgaggattcg gcccaagaca
     5041 acaggcgagg aattgtggta atcggaaata gcgctcgcgc tggtgttctg gttttcacgg
     5101 tagtgggaga aggggattaa agggcgagga ttcaatgacg atcattcgtt ttggaaagta
     5161 gcagcgcttc aattgttcga gattcggaag agccggcgct tttataacgt tttcgaggta
     5221 acgctaggtt ttctccaaca ttcatcactc actcactgaa acaagttgac gtcgataact
     5281 caaaaagggg gcgagttttc gtgtttaaaa aagtaatggc agtttcttgc ttagtcatag
     5341 tcattagtct cttcggattt gcttcttctc tctctacaaa tgctccgcca gatgtccaat
     5401 tatctttcca cgtaaccgca gaattttgga aggatcatat cgcgaatcac ggtatgctta
     5461 tagagattca tttttcccaa aatggaggat atgaagctat caagaaaata tctttgttgg
     5521 gagaattcgg ccgcggcaag ggagaaattg tacttaaagg agaaaacgct tcagccgaat
     5581 tgcttctttg ggatcctaca acacgttttc tggaatactt cattcatctt gacgatgact
     5641 tcgagttaaa aactggaagt aggtatactc taaagtttct cacgactaac ggcttgatac
     5701 agtcttatga ttttatcata gaagacgcgg agagagacta tccgaatatc acttcaccca
     5761 ctctcaacgc gcatttctca accccagagg aaataaaagt ccgatggacg catccggagg
     5821 taagtcgccc gaacgatacc atagagggca aagtaatcga atcctatctc tttctcctaa
     5881 taaagcgaaa aactgaattc ggctctgaag gagagatgtc gagaatacta ttccctgtcc
     5941 cctcgaatcg ttacgaggtc acgattccga gagctaccct tcttaataat agtatgtata
     6001 gaatttacgt tgaatacaca gctcatttca gggaaaatcc gatatattct aagaaagtaa
     6061 gaaactatat tgactttgtt atcggagatt accagtatac gagaatgctc aatgaaaccc
     6121 aaggccagcc aaaactcaat aaagctaatc ctgaagcggg gtaccatttt cctttttata
     6181 catacttcaa tgagaacgct gatacaaacc aaccggttca tatcatggtt cttcctctta
     6241 ttactccaag gccatctgat gatttcaatt acttcttacg atcagccgag gaaacgatct
     6301 tgggtggata cagtcgcttt aacggcctga acatcatgtt tttgtgcccg gtttttcccc
     6361 ggcccgagat agatgcctac gttccttgcc ttgatcataa gactctaaaa cttacgaacc
     6421 cgccagaatt gatccggatt gatttacaat tgatagaaat ggtaaaaata tttcgcaaac
     6481 aattggaaga caaaggaatt gaagttgcgc aaaaactctt atttgagggc tattcagggt
     6541 ctgctgcttt ctcaattcgt tttgcgtata tccatcccga gttagtcgaa gcattctctg
     6601 caggccattg cgccggatgg cctgttctcc cgatagctgt ttacgaaggg aagaccgtcc
     6661 cattcccatt gggaataggc gatttgtata aattaacggg gaaagagttt aatatccgag
     6721 cattgaaaga agtaaaagtt ttcctttatg caggaactgc tgatgataac ttccattttg
     6781 acaacgatta cttagacctg gcggtgaaaa tattgggaag cgaagcgaaa gacatttggc
     6841 caaacgcatt acgaaaatac tacgagaatg gattgtataa tgtgactata cgactttata
     6901 gtggcttgaa tcatagtact acttaccccg cggcaattcc agatgtcgtc cgtttttttc
     6961 agagcgtttt aactaattct cctcaaaagt aaccttttcg gcgacaaact cccgcgggaa
     7021 tttgccctgc gatctttttg ggaatcgtga ttcactcatc gttttcgtaa ggaacaaaag
     7081 taaccgaaac aaaaaactgt tatatttctc atcctgtgcg tggatgcgtg ctggtctatt
     7141 ttctttttaa ccccttacgc gcgattagag atccctggtt tttcagcttt aaagaaaact
     7201 taccaggata acgtccgtcg cgctctggtg gcgacccgaa ggtgttcccg gaaaggcctg
     7261 ccgaaccctg ggagcgccgc gctttagtgc ggtatgtgtt cgatgtttct tccgtgccct
     7321 cgtggtctca gtgtgaaact tttttctttt aatacaggcc aacttaaaag ttagcgttca
     7381 caggcactcc cagggcgttc ccaagcgtat cgcgccgtcg cgaagagaag aagcgcttac
     7441 gccgcgatgc ccgtgcggag ttatcgcggg gtaaccagcg ggctggcttc ggaagctcac
     7501 aaagtctcgt gatacgttct tctgttctaa gacctgccaa cttaaaagtt ggcgttcaca
     7561 ggcgctctaa gagcattcca aagaatcttt gctctgtgtt ctctgtgctc tccgtgcgaa
     7621 ccctgttttc caagacatgt acaaaagcgt atcgcgccgt cgcaaagaga agaagcgctt
     7681 acgccgcgat gcctgttctc tctgtgccct ctgtgcgaac cctgtttttt taattttaag
     7741 acatgccaac ttaaaagttg gcgttcccaa gcgtatcgcg ccgttgcgaa gagaagaagc
     7801 gcttacgccg cgatggccac ataggcgcgt caactgcagg ttgctgggag tgtgctataa
     7861 tcggtaccga ttatacgtcg ctcgggagga tgcggatatg caaatcaccg tggttaatag
     7921 ggctttatcg ttgttctcgg aagggtattc ttgttctcaa agtgtggcgc tggccttctt
     7981 ggaaaactcc cttccgccga gtgtggtcgc cgcgctctcg gcctcttttg gtggcggtat
     8041 ggggcggtcg gggcagactt gcggcgctat cagcggcgcg gtgatggtga tcggatacta
     8101 ctatggccaa cgtgtccctt tcgaagccga gaaaaaggag cgcgcctacg gtttggttca
     8161 acaatgggtg aaagccttta acgccgagtt tggccacacg acttgcaacg ggctcatcgg
     8221 cgtggatgtg gcggacgaga accagcgggc ccgcgccagg gagcaagggc tgttcgacgc
     8281 ccgatgtcct cgcttcgttc aaagagcggc cgagctgacc catcagttgc ttataaagca
     8341 tccgatagcc tatccgcgat aatggaacct cccttttggc cccgctagtc attctggcat
     8401 ttatgcgatt tttctttctc taccaaagcg atcgttaggt tagtcgccgt ttgttcagtc
     8461 ctgtgagacg gggtttccag aagcctgggg atgaagccgt ccgaaaaacc gttgcccgag
     8521 ggacagggcg tacttgtaaa aaaactgcgc tgacttgaaa atcggcgtgc ctcaggtgtt
     8581 ccaaaagaga aatcaataac catgccgaac taaagttcgg cgttcccaaa aagaaaaaaa
     8641 gccaacttaa aaaccggagt tctcaagcgt gccctggtgt cccaaaaaag aaatctataa
     8701 ccatgccgaa ctgaagttcg gcgttcccag gcgttcccag gtgtttcaaa agagaaatcc
     8761 agaaccatgc cgaactaaag ttcggcgttc ccagacgttc ccaaaagaga aataggtttt
     8821 cctcatcatt tttcgattca gaggtggtct ttgtgaaaaa aagcgtcttt ttactactct
     8881 ccttgtgtct gttcaccgct ctcatcgccg ccgaactccc gttaaagatc ggttttcttt
     8941 acgtaggtcc cgttggagac gagggatgga gctacgccca cgatctcggt cggctgtcct
     9001 tggaggcgcg tttcggagat cgcgtcgaaa ccgtctatcg tgaatctgtc ccggaaggag
     9061 ccgaaaccgg cgccaccgtg gaagaattga ttttggaagg ctgcaaggtg atctttggaa
     9121 cgagttttgg tttttcggac gccttgtacg attacagttt acgctatccg gaggttcact
     9181 ttttccagtg ctcggggatc aaggccggcc ataacttgtc cacctatttc gggcggatct
     9241 acgagccgag tttcctcgcc ggtttggcag ccggtagtat gacccggtcc ggaaagatcg
     9301 gttacgtcgc gccggtcgaa actccggaag tgatccgtat cgccaatggc ttcgctttgg
     9361 gcgtacgcgc gataaatccc gaggcccgcg tttacttgcg gtggactggc agctggtatg
     9421 accccgactt ggaagaatcc gcagcggccg cgcttattaa tcgaaaatgc gatgtgatag
     9481 cccagcagac ggactcttcc gctccggcga tgctttccga gcaacgggat gtctgggcga
     9541 tcggatacaa ctccgatatg agccaagcgg cgccgactaa aatgctgaca tcatcggtct
     9601 gggattgggg aatatactac acagagacga tcgaacgtgt ccttgatgga acctaccgtc
     9661 acgtggcttt ctgggaaggt atggattcgg gactcgtcgg gttgacgcct ctttccgacc
     9721 gaataccgga ggaaacccgc aagctggtcc aggactatga gaggcggatg ctgtccggcg
     9781 acttccaccc cttccggggt ccgttatacg atcagggaaa tcagctgcgt atagaggccg
     9841 gtcgcgtgat gaccgatgaa gaactgctat cgatgagctg gttcgtggat aacgtcgtca
     9901 cgagcgctcc ctagggggca catatgccgt cgttcccctc aaagggagtg gaagaggcct
     9961 actgactcaa caactcgatc accgccgccg gcgaaagacg cagcaacgca taggcggccg
    10021 cgctccttat tagactcgct tcggatagat cgcccataat ccgtttcagg ctttcgatgg
    10081 cgaaaccgta ccgcgctttg gccgagtgtt cgatacaagc cactcgtagg cgggtctctt
    10141 cctttttttc gttcatacgc tccacaaagt tcgcgtactc gtctacgagt tttaggcgag
    10201 agatggcctt tatcgccgct tttcgtacta aatagctgtg atcttgtagt aaatctccga
    10261 cgaccttttc cagctcgtag tccatcaaac gcgccaaggc atctaccgca gccgctctca
    10321 ccgtttcatc agaatcctgc agtctttcgc gcagaagcgt caccgtagcg ggctcggcca
    10381 gctttccaaa tcctttcaca gccgagaggc ggattttggg ctcttcggct tccaaccacg
    10441 ccgtgagaaa gctgatggcc gtcgcggggc tatccaggtt tatctcgacg tacagcggta
    10501 tcagcaccgg ataatgcgcg cgaaggcgcg gatcatcgaa ggcttggcgg agtatggacg
    10561 ctatcgcgtc gtagggcagg tgcttgagcg tcgtcaacgc cacttttttt tcttgttcgg
    10621 tatagcgatc cgaagacagc atctcggaaa aaaacttctg tacgaagtca ggccgaaata
    10681 ccaaaagctt caacaaagcg ctctttcgcg cttgcggaaa aggtgtttct tgcgcgatct
    10741 tctccagcgc gacaggatcc tccgtcctct cgataaaggc ttgataggct tcagccctga
    10801 cgctcacatg ccggtcctcc agcaggggtt ggacgtcttc gggcggaagc tccgacgcgg
    10861 caagcttcag cactctcacg cggattttcg gcgaagggtc ctggagtaag acgcgaagcc
    10921 tttctggttc tggatttatt gcgtagagcg cgttcaaagc gctcagccgc acccggtcgt
    10981 ccgggtccat cagcaactgc tcgatgaccg gctgttggtc ttgggtaccc gtctctttga
    11041 tgtattcggc aagcgctttg cgcacgccaa agtttgagtc ctcggccaat ttgagggcgt
    11101 actccgtcca cgtgcccgtc ttcttcaacc cgttgatccc ttgtaaacgg accgaggggt
    11161 gcggtgagtc cagaaggtcc atgtactgcc tactcaaacg cgtgacctca cgcgcctgga
    11221 attctatcac taagtctttg gccattttga aagacatatg ccactccttc attcatcgtg
    11281 cgtgatcgcg ctgatgacca agccgaaccc gcctaaatag gccagcatag aactaccccc
    11341 gtaactgatg aagggtaggg gtataccggt aggcggaaaa actcccagca cgacgcccac
    11401 gttgataatt acctgaatca gcaccaggta cgcatatccg acgacgatga gctttccggc
    11461 aggacgattg actcccttga tggccgcgtg gatcaggctc ctcgccatca ggtagtatag
    11521 tatcaacaga aaggccatcc cgacgaatcc taactcttct cccaaaatcg cgaagatgaa
    11581 gtcggtgaat tgaacaggta agaggtattt gtagtctcca agggccagac cgcctcctaa
    11641 aacccctccg gaggaaatcg cgtcgatccc gatctgtacc tgcgtgtcta cggcgctgcc
    11701 cgtaaaaaaa tctaagaacc cgcgcaaccg atccagctga tagggtttta gaagctcaaa
    11761 ctgtaaaacc gccaccaacc cgaccacagc caaaccggcg agcaagaaaa agtaatagta
    11821 gcgaagacct gcgacgaaca tcaacaaaaa tccgatggac aggatgatca tcgacgtgga
    11881 gagatccggc tctcggaaga tgaggtaact gatgaacagg atccaaccga ccgggatgag
    11941 aaatttatcg aagaaatcga ccttccaatt ttcttggaat tggcgtttgt caaagtacgc
    12001 ggcgaggaag aggatgatca cgaatttggc caattcggac ggctgcaggt cgtagaaccc
    12061 gaggtctatc catcggtgac tgccgccctt tgagggaaaa aaaaagactg ccaccagcag
    12121 cagaatcaga cccaccaagt acccgggcgc ccattgcaag tgtttttcga tgggaatcat
    12181 cgctatgacg aacataaata caagcccgat cgagatgttg agaacctgcc gggtgaaata
    12241 gtaggccaga tcgatgccgg gccagcggta aaccatgatg tagctggcgc tatagaggat
    12301 gattaacccg aacactagca gaatcgataa aaagtaaaga acccctttga agtttacgac
    12361 tcgacttcga gtcccctctt gttttcccaa tttaattcca acgcctttcg ggtgatgaaa
    12421 tgaaagtctt ccaccgttct gaggcaatat aaattgctgt ccaccttcac gcaatccgtg
    12481 gcgccaaagg atgggaccgc tttgaaaaat ctcgatacca tctcggatat ctcttcttcg
    12541 attttgttcg tattcggcaa caacaacgcc attttgatgt tcccgtatac taacagccga
    12601 tcgatgtgcc agtgttcgac gcgttttcct ttcgggtttt ccgcgaatct gtccttgtaa
    12661 tcgatatgcc ggtgaacgcg ctgtcgcaaa tggttcatcg ctgaccccac atagacgtaa
    12721 taccctcttc tgagatgctg tttgagggag ccgtagtgga tctcgcgtcc tttgttcagc
    12781 tcaatcaaca gtaggtagct tccggtatta ttcgccattc ggtatgcctc tttcttccgg
    12841 attctgtttt tccgaaacta cggagtcggg ttgcgctccg ccgtcctttt cgatcatact
    12901 gaggtctgcc gcagccataa acagttggtc gatgctcttc aaaaaccgta atcgcgtcag
    12961 ccgaaggtct tctcgatcca ccatcacgaa cacgtcgtca aaatagcgat cgatggggtt
    13021 tttcagctcc atcaggccga tgagcgcgtc gcgatagttt aagcgccgga tgtgcgagag
    13081 tacggtgtct ttcgtttccg tgtaccgaat atacagctct ttctccgctt cgttttcgaa
    13141 aagccgcgag tcgaagtcgg tgtctgtcac attccttgtg atattgtgca ctctctcaaa
    13201 cagtgtggaa atttgtaaaa agacgggctc ggttcgaaac tcttgaagat tctgggcgac
    13261 gatcgccgct ctcatcggag tggcccattg gtgttgtacc gcccgtataa tatcgtaggg
    13321 aagacctttc ccttgtaagt aaaactccag cctgctttgc gtgagctctt tgaacgtctg
    13381 aagcagcgca tcttcggaac gttcgggacg cgcggtcgaa ggggcggttc cggaagggcg
    13441 gtcgctgacg aagggtttga ggccttgccg aaagagatcg tccaggcgca ggtcccagtt
    13501 gtaagagatg tatgtctcga tgatttggta cagctttctt ctcagcccgt acgggtcttt
    13561 cgaaccgctc gggacatttc cgagccagag gttgccgcat agaacgtcca tcttgtcggc
    13621 gaccgagagg accgcgcccg ccacggactc cggtaccgat tgactgtact gctcttccaa
    13681 cgcctttgag atcgcgggat cgagaccctc caaatcggcg tagacacggc ccatcgtccc
    13741 ctgtaactcc ggaaattcat agacgacgga ggtaagaagg tcgcttttcg agagtcgcgc
    13801 ggcctcgtcc atcaatgccc gttggggcgc cggaaatccc aagctctcac acagataggc
    13861 ggccaagtcg cggttgcgct cgactttgtc aaaggttgat cccaaaccct tttggaagag
    13921 gacgtttttc aagtggttca gtcggtcgcg cagcggagtt ttttgatcct cctcgaagaa
    13981 aaagcgcgcg tcggcgagtc gggcgttgat gaccttttcg tagcctttca ccacgttcgc
    14041 cttcgcctca tcgccgttgt cctgaaaaga gacgaacccg ccggtcaata gcccgtctct
    14101 cagaacgggg aaggttcttt gatggtgttt tagagtggtg atcaacacct ctttcggaag
    14161 ggttttgtag tccgaacgga actgccccat caccgcttgc gggtactccg tcagccaggt
    14221 cacttctttc agaagcgcct cgtccaccgg tatctccaca gaacaagttt tcgcgaggtc
    14281 ggtcagctgt tgtcggatga acccttctcg ctcgtcttgc cgagcgaaaa cgtattggtg
    14341 tctcatcttg tcgaaatagt cttcagcacg gtcgatcgtc gtttccgcac ccaagaaacg
    14401 atggcctcgc gtcgttcgac cggaggtcac tccaaagcag gagaacggaa cgatttcgga
    14461 tcccagcagc gccaataaac cgtggaccgg cctcacaaaa ctcgtttcgc cggcgcccca
    14521 acgcatcggc cgatgaaaac tgagtccgga caggatcgta gggacagcag aagctaaaag
    14581 gtccaccgtc attttccccg gaataatgcg ccgaatgaaa acgtatggga cgccgttgat
    14641 ttcctctatc tctatttcgt ctacttttgc tccgttgctg gctaggaagc cgttcaaggc
    14701 tttcgtcggt tcgagtcggc tttcatctgc gaaggccact ttgcaggccg ggcccttttt
    14761 tcgctcctct cgatcgggct gacgcggttc caaacccgat accataatcc caaatcttct
    14821 cgaggctacg aaggattcca ttccggtagg gcgcaatccg aaatcgataa aacattgacg
    14881 gaatttttct tccagattgc tcagtagccc cggtatttcc gtaggaggaa gctcctcggt
    14941 caatacttcg agtgtaaagg tcaaacccat accgcgaata cctcccgttt ccgtatcttc
    15001 ttgaaaacaa cttctcgctc gggtcactac ctcactacag ggaagcagag tgtccctttg
    15061 tctttgcgga cttgcgatag aagatgcccg ttcttgattt tctgctcgcg atatgattat
    15121 acgaaacgcg tcaagcttac ccacaatttt ttcctctttc gttagggcga gaggtatgtc
    15181 ggaagaattg agcatcatag agatcggtaa ataatccgga ttctcagtca ttggctttaa
    15241 ttcggagaaa cccatcaaag aaaatttcag ggggcggtcg gaaatctttt tccggttccg
    15301 tttgtccttt ttcacgacaa acagaatctc tccctctacc ccttccgaaa agggggcgac
    15361 ggcgaccttt tccaaaaaac gggtgttctc ctcgggttcg gaatcggtgg ccgggatgat
    15421 atccacaaaa gcattcgttc cgggcgttcg attcgtaact ggagagcccc tgcttacctc
    15481 aggcaaggaa gcgtcataga ggtgaagctg atagggtatc ttttgtaaag cgaaccatcc
    15541 ggtcaagagc gcggcgaacc gcggtaaatc agtgctccat gagacgtttc gttcgagata
    15601 ctgacgaagc tccagggcca cgttctccgg actcttttcc atctcgagca tcggcttggc
    15661 cacctttttt tcgtaaaaaa tggtgtaaaa gcccaaaccg agcgccccga aaacgatgaa
    15721 tgaatcggaa acgttgaata tcgcgaagtt ccaaaaagac agaaaatcaa tgacccaacc
    15781 gtcgcgtaga aagcgatcca gcaggttacc gagcgcgcca ccgacgatac taccgaaggc
    15841 aaaacccagc agataagact ttcgcgcata gcgaaggccg ataaaaaaca cgaaaatcgc
    15901 gatcgcaaga acgatcaagc gcagggagtc cccgtgccct tgaaagaggc caaaagcgat
    15961 tccgctgttt ttgacgtgtg tgatcttgaa gacctcaggg atcaccggga gctgctggcc
    16021 caccatcacg tggtgttcga cccatagctt cgaagcctga tcaataaaga gggctaaaac
    16081 cagccaaaaa cccatattca tcatttctga gcacgcacgt tctttctaga atagaagatc
    16141 agttcagcct ctatgaaggc gtcgagatcg ccgtccatga ccgcggccac gttgccggtc
    16201 tcggcctctg tacgatggtc tttgaccatc ttgtacggct ggaagacgta ggaacggatc
    16261 tgattgcccc aggaaatgcg gttgagttct ccctggatat tgctgatctc cttcttttgt
    16321 ttctcttctt ccaattgata caacttggcg gccaagattt tcatcgccgt atctttattt
    16381 tggtgttgat tgcgttcgtt ctggcaagcg accacgatac cgctgggcaa atgcgtgatc
    16441 cggactgcgg attcggttcg gttcacatac tgtccgcccg ccccggacgc gcggtagaga
    16501 tcgatccgca gatcctcggg atcgatgtcg acaacgatgc tgtcatccaa ttccggtaag
    16561 acgctcaccg aagcgaagga ggtctggcgg aggttgttgg cattgaaagg cgaaaggcgc
    16621 accaaacggt gtatcccctg ctcgcgccgg atgtgtccgt agacgtatgg gccttcgatt
    16681 aagagggtga cctctttgat ccccgcctct tctcccggtt gcaactctat gattctcgtc
    16741 gtgaatccct ttctttccgc ccaacgcagg tacatacgca tcaacatttg cgcccaatcc
    16801 tgggattctg tcccgcctgc gccgggatga atcgttaaaa aggcgttgtt ggcgtcgaac
    16861 tcgtcatcca agagcatcgc cagctcgtat tccttgactc cacgggctac cttccgaaat
    16921 aactccaaag cctgctcttc catcgaatca tcctcggccg aaagctctag agcggcttgc
    16981 gcgtcctcaa tcaagctctt caacgaagaa aactctccga tctgcgcctt tagcgatttc
    17041 acctctttca gaacctgcgc ggaacgcgtc gtctccgtcc aaaaatcggg attggccatc
    17101 tcctcttcta actggcctac ccgggcttgc gccccgtgaa ggtcgaacag ggtcgaaagc
    17161 tccccgaact tgcggaggtt ctcttcaaat tcgcttttac tttcgtaggg tatcataagc
    17221 gttacctctt tactttcatt cttcgatgcg ctgcggacgc cttgtcctcg acttttcggc
    17281 gcaattttcg attgaagctg tcgaactcgt cgtgtcgata gctgagatcg tcaatctctt
    17341 tttgagattt tctcgtttct tcttcgtact tggtcgtgac ccgaactctc aacaggaggt
    17401 tggccatatc gtcatagatc gcgtcggtca tctcctcgaa gaggataaat gactcccttt
    17461 tgaactccaa aaccggatcc ttttgcccgt atgcgcgaag gctgatcccc tccttcaagc
    17521 cttcgatgct ttccaggtgc tttcgccagc gctcgtcgac gacgcgtagg agcagatact
    17581 tctgtaggcc gacgaaatcg gcgccgatct cgtcacgttt ggcttggtag gcttccataa
    17641 acttcgcgag gagacgttcg gcgatctctt cctttcgatt gaaagcgcca cttttaatct
    17701 cgtcgaagtc gatgggaaag agtttgaggt tttggagcag cccttccagg tcccagtcgt
    17761 tcgtgtcgca gtattggcta attcgacgat cgacgatatc gcgatagatc tctttcatat
    17821 gctcggaaag gtcttctttg gaaagaatcc aatctcggtg ctcgtaaatc tccttgcgct
    17881 gcgagtccat aacggtatcg agctcgagca gatacttacg tgtggaaaag tgcagtcctt
    17941 ccacgcgctt ttgcgcctgc tcgattaaag aattcaacat aggatgggcg attggctctc
    18001 cttcctcaat cttgacgaag tccatgatct tcccaattcg ctctcccccg aagatgcgaa
    18061 ccaaatcgtc atccacggcc aaaaagaact tcgaactccc cgggtcgccc tgtcgtccgc
    18121 agcggcctct cagctgattg tcgattcgac gcgcttcgtg gcgttcggtg cccaacacgc
    18181 acaaaccgcc cagttccacg acaccctctc ccaacttgat gtccgttccg cgtccggcca
    18241 tgttcgtcgc gatggtgatc gcgttcttct ctcccgccaa cgcgatgatc tcggcctctt
    18301 tctcatggta cttggcgttc aacacctggt gaggtagtcc caaacgggtg agctctcgag
    18361 acagaaactc gttcttctcg atcgaggtgg tgccgaccag cacgggttgt cctttcttat
    18421 gacgttcggc gatctctttg aggatcgcgc cgtatttttc tttttgcgaa cgatatatct
    18481 catcgtcatg gtctttacgc acgattggca tattggtcgg aatcacgacc acatccgtca
    18541 cgtagatttg ttcgaactct tcctcttctg tcttggcggt accggtcata ccggctaact
    18601 tctcgtacat ccgaaagaag ttttggaagg tgatcgtggc ataggtcaac gactcttcct
    18661 gcacgcgcag gcgctccttc gcctctaagg cctggtgtag tccgccgcta aaccgtctgc
    18721 cgggcatcaa tcgaccggtg aattcatcga cgatgatgac ctcgccttcc tcattgacga
    18781 tataatccac atcctttttg tacagctcga tcgcgttgag cgcgttcaag atatggtaaa
    18841 gctgatcgat attcccgggg tcgtaaaggt tttcgatttt gaggttcttt tcggtctttt
    18901 tgacgccttc gtcggtcagc gtcaccgtcc ggctcttttc gtctttggtg aagtcgacgt
    18961 cctttttata tccggtcgcc atacgcgcga acacgttgta tatctgagac gtgcccttcg
    19021 t
//
LOCUS       DAOPXN010000024        18393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3074_length_18393_cov_5.175101, whole genome shotgun sequence.
ACCESSION   DAOPXN010000024 DAOPXN010000000
VERSION     DAOPXN010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 18393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18393
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_18393_cov_5.175101"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1157
                     /locus_tag="PKM49_02460"
     CDS             39..1157
                     /locus_tag="PKM49_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HNY81711.1"
                     /translation="MIYDSPIEVRHTLHQNPEKTLQEHETTEYLFRELSKVEGITLHR
                     PFLLGLVAEYRVNDGDYLLFRADIDALNIKEETGWEFASKNDCMHACGHDVHTAILYG
                     LALTVVEKRPNQNVLFFFQPAEEGGGGAQKTVDTGFFDRYAIRCSFALHVNDDYPFGS
                     VATNDHVLFASSSEIDVLFQGVGAHVAYPTEGKNTLLALRYFLDAIDRMPREVEKPFL
                     FSAGKVAAGEVRNIQPAKAFIQATMRTVSTEENDKMFDQIEKMAEHIEKLTGVEIRVK
                     RLSVYPEVKIDKGLYDRLIPKLRERYQVLKAPMKMVGEDFGVFCHLYPSILFWLGTRR
                     EAFYPLHHPKYLPSDDIIPVGIEFLYDLFTFLSDEPAL"
     gene            complement(1126..2619)
                     /locus_tag="PKM49_02465"
     CDS             complement(1126..2619)
                     /locus_tag="PKM49_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNY81712.1"
                     /translation="MEGSRINANFSKWNVDRWIDFPLFWYAAALWCGLLLEGTLSHIV
                     GLSALILLLLIGLLLRKRAKTASLRASSTEGTTTQVGRYLLFTLFVGAFFARGVIPGF
                     FGADHSFALDQQVSAVGRVQSVTEKSLRLTEVRVLEDRVWKAVKESVYIYRDAKGEYD
                     PGDWVGFKGRITMVDPIVYVSILYPSGAFHYPYRTGPVQQVLSFGHGIANAYAEHVQS
                     VLPAEEAQLAAGIFLGKRFEGDLRIWMNDSGLGHLFVVSGLHFAMLSQIGLILLGFFR
                     VTDPLRTLLVLCFLGFFWIVTGCPASSSRAFLIILFYGLFRMFSYPASGLNLLGAAAI
                     LLLLFQPTYARDVGFALSVLATVGIYCGHRVSLRTNGRVGRYLLPLTGAMVFTTPITI
                     AQFGRMPFLSIPFNMLFVSSVAMVLLIGLLTSFLCFLLHLPGLSAIFLHGLSPFIRLN
                     TAILSFLGKHGGTLAVPNGLSIPFWALAAVFLFTALSIKEPVRPTKT"
     gene            complement(2561..3085)
                     /locus_tag="PKM49_02470"
     CDS             complement(2561..3085)
                     /locus_tag="PKM49_02470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HNY81713.1"
                     /translation="MISFRPLPDGFETGDAVIDLLPFFDRTVWIEWLEHAPGLPMGGL
                     VQARFLNRYTKPTRIRSQGALQFWEYLARERQTWEMVLLYPEGFSPFFLEVYERLLQD
                     VPYGDTLSYSALATRVMRPNSPRAVGLAMSKNPWPLVVPCHRVVTKTGQLGGYGGGIE
                     NKRKLLEMERRSMD"
     gene            complement(3082..4125)
                     /locus_tag="PKM49_02475"
     CDS             complement(3082..4125)
                     /locus_tag="PKM49_02475"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNY81714.1"
                     /translation="MDNLQPIHRKQGGSRVNQRAEFRKGYMGPTIPAPPQPKKPKAPN
                     RIGLSALQGQLLAIVVIALALAVVLMGFWINRLLTERQDKNLIIQNLSSRVQNLETVA
                     QEYETTLSGTHSASYRPLPTDLTAPIAPGIENPDMAKKESFEFERFFKNAQTTIYEGK
                     PLTFYICNDQAALRLIRDSQLPYLLSAYSDNEYSLVLIGRYEPAWVIQLDNLQKIRRE
                     FMSQLITLSASPTRTGTATVLPSIPEIPEDLQGLELRRLYTLYNIQISADSSLEKLKT
                     QVGILRNEGIPAFVYGYSSSGTNLLYSLRIGLFPTDQDAKKYSERMDRVLYRELIHKE
                     ITDRFIRQLAIPQ"
     gene            complement(4238..5275)
                     /locus_tag="PKM49_02480"
     CDS             complement(4238..5275)
                     /locus_tag="PKM49_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81715.1"
                     /translation="MKEIFTYSVVKELQTRCFLFLGERESGFQDRILSEEHTYTVIDT
                     KVKSAIADLQTLYRKAYPVSAGEHCKSIDRVFELYTVLQTLSPRAQMINVIGGGALID
                     LVGYALATSFQDLKMRAIPTTPFSQLYGFYQRSFYIHFDRKINQLSVFGIPHELWIIP
                     FFLKAFAVEDLRKAHVVCFATALGFEERFFYLVERSLSLLIHNQTNWTVFYELLREHH
                     FLRAKCSLEHRLIPGESMGILLQTATGFQMDFVDAFIKGLMLEAYISFKLGIIEESTL
                     EMIRKTLSQIGLEDRLPPQRQVDLQTLFSLIDEKKSIKMQVIKGLGLSQETTVSATII
                     EHILKEYYFGR"
     gene            complement(5332..6345)
                     /locus_tag="PKM49_02485"
     CDS             complement(5332..6345)
                     /locus_tag="PKM49_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY81716.1"
                     /translation="MKELIKKLTQVSAPSGRERKVHLAILEELEGFIDGYRFDNVSNL
                     IVWKNGNGENKKNFIFDGHADEIGVVITNIDEKGFLRIDPVGGVNPLMLLGTLLNFDD
                     VTGIVSYEGETDEDAKKSLTQPSYDYLFVDIGATSREDAQKKVHIGQFGSYLSPFVDM
                     DPMLVSKSMDDRIACAIVVQAIKETQNPYHNIYGIFSVQEEVGILGAKVAAYDIHADI
                     AIAVDVTGHTDTPKGNKRMDLQLGKGPAIKIKDKASISDESVVRLLTKCAKANQIPFQ
                     YEVLIYGGTNAHGYQLTGAGIKSGTLSIPCRYVHSPHEMVHLEDVKNAVRLLNAVALK
                     SDW"
     gene            complement(6342..7379)
                     /locus_tag="PKM49_02490"
     CDS             complement(6342..7379)
                     /locus_tag="PKM49_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNY81717.1"
                     /translation="MFLKDLCEINGASGNEDRVRSYIQEQLKSPEEPGQVDVLGNLFF
                     EKKGNGTSDKTILLMAHTDEVGLMVTNIQDDGLLAVSPIGGVDPRVLLGKRVWVGENN
                     LPGVIGYKAIHLQKEDFKTLPDWDKVRVDIGAQKKEEAQKSVNLGDYVHFYSPFEEYE
                     NRYVGKALDDRAGCALLMALYRELPVLNHRVVFAFVVQEEIGLRGSGSTLKRYKPDAA
                     IVVEGTTAGDNPELKRERWSTHLDNGPAISYLQSGYALDYRLFEAIVAFANQKGILYQ
                     LKGRTVGGTDGTRLASTYFGVPCAGVNIPSRYIHSPVSVISKKDFENTKQFIADILTE
                     EILLKALEVKV"
     gene            complement(7381..8397)
                     /locus_tag="PKM49_02495"
     CDS             complement(7381..8397)
                     /locus_tag="PKM49_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HNY81718.1"
                     /translation="MSLDTFAALKSLSLAYGPPGREKEVRGLFKGYVNGLVDEVFEDT
                     SGNLIVKRYGQHKGTVFFSAHMDEVCFLVSKVLPEGFLRLEGIGADVKLLPSQKVWIH
                     TRSGKRLRGVIGMLAPHLQTEETRKKLNGFEDLFVDVSMSDFSEISVGDFVTYDNEPF
                     STGDFFFGKSLDDRACGVIIVKMLERLTALRHDFDVFAAFSSREEIGAFGARTAAYAL
                     QPDIGIALDVTHAIDTSPNYPKNEFGKGPVIAVGAIPHRKISNLLIETAKQNGIPYQI
                     EPSPSRTGTDTDQIQLETVAAGVVSLPLRYMHTPYEQICVKDIDETARLLALFVSALK
                     EGVV"
     gene            complement(8399..9088)
                     /locus_tag="PKM49_02500"
     CDS             complement(8399..9088)
                     /locus_tag="PKM49_02500"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNY81719.1"
                     /translation="MVDLRFDGEDLILFVGSNTEIDELEAFLEEKIGLLSDFNNTASK
                     VTVWFEDETEKPGQIQLIGSRLRQAGLSVKAYSFEKPLARHPGANLTPTARKPITSQE
                     PFPLETSTLKPLKVVEGSLRSGQSEDYDGHVFVMGNIHHGSELKAAGSVIVYGKIHGT
                     VHAGKADPENSFISALGLVNAHLRIGEVFSYSLVSEVCVVCVAKNGRIHQIPIEKQET
                     PNIFGKIGRVR"
     gene            9243..10550
                     /locus_tag="PKM49_02505"
     CDS             9243..10550
                     /locus_tag="PKM49_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNY81720.1"
                     /translation="MKAHFIGISGIGMSAAALLAKDLGYTVSGSADAENDRTELLRDR
                     GIDIFIGHRADNVQRPDLVVFTNAVSEHNEELITVRALSIPALPRLTFLGSVLPRYPK
                     PVIGITGTDGKSTTTAMVAHMLLENGEDPTVLLGGLHQDLEEGNYRRGKGPLVMEVDE
                     SDGFLRDFSVDVACFTNIHGDHLEHYEKDYSHYRDAIFDFVCHARERVFPASYADAYR
                     SYCNSRGITPGKTHLFELDPQIERWYHHTIPGIHNRLNASAALKVCEVYGLSRERAIG
                     CLENFPFVDRRFSIRYESNRVIVIDDYAHTPREIESVLQAVNERFPESLPHLVFEAHR
                     YSRLEREFHDFVAVLSSRQIQKTHILPIYSAYEETKPELFERFLVAMRQNRPDTVFIE
                     DATQIVPTIPVSPTAPVVYLFVGAGHSSLHSKALASYLERHHP"
     gene            complement(10574..11605)
                     /locus_tag="PKM49_02510"
     CDS             complement(10574..11605)
                     /locus_tag="PKM49_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY81721.1"
                     /translation="MKIDFSYKKQPIRFLSGYIFKGINYRCSYIRYDTLYRNPAPGTE
                     TISLYHFHPKTKAKATILILHGLGSGNIKFLLWMGMHLAAAGMNAEVLILPGNYTRVE
                     KGSMSGRSYVWPDISVMYRFWEHAIVDIQTTIDFLEQEGLWLENNCISGYCLGGMLAS
                     MVPVLDQRIHQTVIIATGGYFPKILYESSATRFARKLFREGYQTDYSLHDPKALFSIY
                     QQQYGFVKKADLETLLTSEAIHPLFRIDPISYAHLLDKNRVTFIEAFFDKTLPRTSRQ
                     LLYREMKGANRYILPTGHTWWLPFQILYARYLLHRMNAYSRKSSKALLKMETIEEPVN
                     DIEGHTHTH"
     gene            complement(11595..12635)
                     /locus_tag="PKM49_02515"
     CDS             complement(11595..12635)
                     /locus_tag="PKM49_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNY81722.1"
                     /translation="MLKTAIKAFKKSWFFVNKLYLLIPFLYFLVFDFSSAIVGFLVFS
                     FLFLIRILTQKVPLDPLKEFEKRVVVYKKTDVGDLKLDLWYPENGRAAYPTVIYAHGG
                     GWISGFRNQPNNVSWCRYLASKGFCAVSVGYRLGILKSIDEIMSDFSDAIAYTREHAD
                     ELRVDPDHLFLMGLSAGGHLALLYTAYQTFHNVREQLSGIRGVIAFYAPTNLRDLFEK
                     DSKSFFARFATLLTLRGRPGSIEELYQEYSPIYWLSERMRPTLLVHGKEDTTVPFSSS
                     VQFARELKRLKVPFRFLVHQTGNHTFEFSQKDVKTDWILEQTVRFLRAYLKDRKPTPS
                     LLNEELRKHYEN"
     gene            12822..13166
                     /locus_tag="PKM49_02520"
     CDS             12822..13166
                     /locus_tag="PKM49_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81723.1"
                     /translation="MAEKRKGLIVGGAAQARPASLGSELAIRAISSFYSEIGLLGLWG
                     SCPDRSFWGIPLLGLSAEVFFRAPDTKPLTGICLGPNDRILSSALRERWSFIGLTDCH
                     GIGLRGFAYELG"
     gene            13325..14257
                     /locus_tag="PKM49_02525"
     CDS             13325..14257
                     /locus_tag="PKM49_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HNY81724.1"
                     /translation="MSSKELENKSVEELKEMAKKVKVKGYYKMRKSELVEALSEEWTG
                     SVDEETRVSILAEIEKDPKIYELRNMAKRLGITFQRNARKEELKERILRLLARPKLEI
                     KRYERSSGPAPEKIVPVSTSSTVDLPTTYDKDTLQGLPVNPSWLSFYWDFSKQTDGLV
                     DRFARENRPLLLRVYDVTFLIFNGSNAHRTWEYRINGKEKKYYVQVALPAASYLAEIG
                     YYNDTGVFVPLLRSNLVNTPAASLSNRQEERWIDLRKHLRFSQTHISLASRPNLLPTG
                     LSSLTTEELRGSFSSILFSSREVQKRTSDDRSKE"
     gene            14270..15874
                     /locus_tag="PKM49_02530"
     CDS             14270..15874
                     /locus_tag="PKM49_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HNY81725.1"
                     /translation="MTKGYLTLVLHAHLPYVRHPEFEEFMEERWLFEAMTETYIPLLR
                     GFEALETDGVPFHLTMSLTPPLMEMLEAKDLQEKYIRHLEKLIELAQKEVEQTRAEHP
                     TKHKMAQYYLKDFQEKLTYFRDRVGCRLLESFKRFQDNGSLEIITCNATHGFLPLMSR
                     YPQAVKAQISQGVKTYEKYLGRKPSGIWLAECGYVKGIEKPLADEGLSYFFVDSHGLW
                     YAENPPRYGVYRPVLTESGVFVFGRDPESSEQVWSGEIGYPGDFRYREFYRDVGYDRE
                     LEYIASYIDRSGARVNTGIKYHRVTGKNVDLGKKDYYDVEEAHRVAYEHAKDFIAKKS
                     AQAEALYDQFAGVQPIIVAPFDAELFGHWWFEGPKFIENLFRCMNETEVIAPISPKDF
                     IDDVLTSVQITTPADSTWGANGYNEVWLNGSNDWIYPHLDEMAERMTRLANDFYNEVD
                     PVKIRALRQAMRELLLAQSSDWAFIMSTGTTVEYAVNRTKQHIFRFNQLYEALYKGEI
                     DEACLGYYEWIDGIFSDPDYRLYALR"
     gene            15934..16008
                     /locus_tag="PKM49_02535"
     tRNA            15934..16008
                     /locus_tag="PKM49_02535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15967..15969,aa:Arg,seq:cct)
     gene            16020..16096
                     /locus_tag="PKM49_02540"
     tRNA            16020..16096
                     /locus_tag="PKM49_02540"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16054..16056,aa:Asp,seq:gtc)
     gene            16251..16916
                     /gene="fsa"
                     /locus_tag="PKM49_02545"
     CDS             16251..16916
                     /gene="fsa"
                     /locus_tag="PKM49_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNY81726.1"
                     /translation="MKLFLDTANLQQIQKAKDWGILDGVTTNPSLIAKEGVRDFHGHI
                     RRICEIVEGPVSAEVIALDVEGMLKEARELAKVHEDVVVKIPVTPAGIQAVGVLSREK
                     IRTNVTLVFSAGQALLAMKAGATYISPFLGRVDDIGWESSDLLEDLLTLIDAYGFDTE
                     VIAASVRHAKHVVEAARMGCHIATIPYQVLEALFHHPQTDNGIDRFIKDWNQLQDRLR
                     KEG"
     gene            17474..>18393
                     /gene="rho"
                     /locus_tag="PKM49_02550"
     CDS             17474..>18393
                     /gene="rho"
                     /locus_tag="PKM49_02550"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNY81727.1"
                     /translation="MEVDIRKLNNMNIKDLYTLAKKYSINGYTGMRKNDLVFSILQAQ
                     AKSFGYFFNEGVLDIIPVEGYGFLRTGNLLPGNNEDIYVSQSQIRKFNLNAGDIVSGQ
                     VRAPKEGEKFFALLRIEAINHNPPDRIYDRINFENLTAIYPVNKFKIENDPQKLSARI
                     IDLFCPIGKGQRGLIVSPPKAGKTTILKDIANGIAKNHPETLRIVLLIDERPEEVTDI
                     TRSVDAQVLAAPFDMPPEKQIKVAQLTLQMAKRLAEYKYDVVILLDSITRLARAYNNY
                     IPPSGKLLSGGVDPKALYGPKYFFGAARNI"
BASE COUNT         4480 a         4829 c         4901 g         4183 t
ORIGIN      
        1 cgcggacatt atatccttgc aagaaacgga ggaaaagtat gatctatgac agtccgatcg
       61 aggtcaggca caccctacat cagaaccccg agaaaaccct gcaagagcat gaaaccaccg
      121 agtacctgtt tcgagaactc tcaaaagtgg agggcatcac gctgcaccgt ccgtttttgt
      181 tgggcttggt ggccgaatac agagtcaacg acggagatta tctcctgttt cgagccgata
      241 ttgacgcctt gaatatcaaa gaagagacgg ggtgggaatt cgcatctaaa aacgattgta
      301 tgcatgcgtg cgggcacgat gtccatacgg ccatcctcta cgggctggct ctgacggtgg
      361 tggagaaaag gccgaatcag aacgtgctct tcttcttcca acccgcggaa gagggcgggg
      421 gaggcgctca gaaaacggtg gacacgggct tcttcgaccg ctacgcgatc cgttgcagct
      481 tcgccttgca cgtcaacgac gattacccgt tcggttcggt ggcgaccaac gaccacgtcc
      541 tcttcgcctc ctcctccgag atcgatgtgc tcttccaagg tgtgggcgcg cacgtcgctt
      601 accccacgga agggaagaac accctcctcg cgttgcgtta ctttttagac gcgatcgatc
      661 gtatgccgcg agaagtggaa aagccattcc tcttttccgc tgggaaggtg gccgcgggcg
      721 aagtccgaaa tatccagcct gccaaagcct tcatccaggc cacgatgagg acagtatcga
      781 cggaagagaa cgataagatg ttcgatcaga tcgaaaaaat ggccgagcac atcgagaagt
      841 tgacgggcgt ggaaatccgt gtgaagcgac tatcggtcta tccggaagtt aaaatcgata
      901 aagggctcta cgaccgcctc atccccaaac tgcgagaacg gtatcaggta ctgaaagcac
      961 cgatgaagat ggtgggcgaa gacttcgggg tcttctgcca cctatacccg tccattctgt
     1021 tctggttggg cacccggcga gaggcgttct atcctttgca ccatccgaag tacttgccct
     1081 cggacgatat catccccgtc ggaatcgaat ttctctacga tctgttcacg tttttgtcgg
     1141 acgaaccggc tctttgatcg agagcgccgt aaaaaggaag accgccgcca acgcccaaaa
     1201 ggggatcgat aacccgttcg ggacggctaa ggttccgccg tgctttccca aaaaagaaag
     1261 aatcgcggtg ttcagcctga taaaaggcga gagtccgtgt agaaagatcg cggaaagccc
     1321 cggtaaatgg agaaggaagc ataggaaaga ggttaaaagg ccgattaaca agaccatcgc
     1381 cacggaggaa acgaagagca tgttgaacgg gatcgacaag aacggcatcc tcccgaattg
     1441 cgcgatcgta atcggcgtcg tgaacaccat cgctccggtc aatgggagca ggtatcggcc
     1501 cactctcccg ttcgtcctga gggaaacccg atggccacaa tagatgccga ccgtggcgag
     1561 gacggagagg gcaaaaccga cgtcccgcgc gtaggtaggc tgaaaaagca ggagcaagat
     1621 cgcggccgcc ccgaggaggt tgagcccgga tgcgggataa ctgaacatcc tgaaaaggcc
     1681 gtagaacagg atgatcagga aggcgcgcga agacgacgcc ggacagccgg tcacgatcca
     1741 gaagaagcct agaaaacaga ggaccaataa ggtgcgaagg ggatcggtga cacggaagaa
     1801 gccgaggaga atcaatccga tctgggagag catcgcgaaa tgaaggccgg aaacgacgaa
     1861 caaatggcct aaccccgaat cgttcatcca aatgcgcaaa tctccttcga agcgtttgcc
     1921 cagaaagatc ccggcggcca gctgcgcctc ttcggctggg aggacgctct gcacgtgttc
     1981 ggcgtaggcg tttgcgatcc cgtggccgaa agagaggact tgctggactg gcccagttcg
     2041 ataagggtaa tggaaggcgc cggatgggta caagatcgag acgtagacga tcgggtccac
     2101 catcgtgata cgccccttga aaccgaccca gtcacccgga tcgtactcgc ctttcgcgtc
     2161 ccgatagatg taaacggact ctttcacggc tttccagacc cggtcttcca gcacgcggac
     2221 ctctgtcagg cggaggcttt tctcggtgac gctttggacc ctgccgaccg cggagacctg
     2281 ctgatcgagc gcgaaggaat gatcggcgcc gaaaaacccg gggatcaccc ctctggcgaa
     2341 aaacgcgccg acaaaaagcg tgaacagcag atatcgacca acctgtgtgg tggtcccttc
     2401 cgtagaagaa gcccggagcg aagcggtttt cgcgcgtttt cggaggagca gccctatcag
     2461 taacagcaag ataagggcgg agagcccgac gatatgagag agtgtccctt ccaggagtag
     2521 gccacaccac aacgcggccg cgtaccaaaa cagggggaaa tcaatccatc gatcgacgtt
     2581 ccatttcgag aagtttgcgt ttattctcga tccctccacc gtacccgccc aactgaccgg
     2641 tcttcgtgac cacgcgatgg cagggcacaa ccaaaggcca agggtttttg ctcatcgcca
     2701 ggccgaccgc tcgaggagaa ttaggtcgca tgaccctcgt tgctagtgcg gagtacgaga
     2761 gggtgtcacc atagggcaca tcctggagca gtcgttcgta gacttccaaa aagaagggcg
     2821 aaaagccctc cggatatagg aggaccattt cccacgtttg tctttccctc gcgaggtatt
     2881 cccaaaattg aagggcgccc tggcttctta ttctcgtagg tttagtgtaa cgattcagaa
     2941 atcgggcttg aaccaatccg cccatcggaa ggcctggagc atgttcgagc cattcgatcc
     3001 agacggtacg gtcgaagaac ggcaatagat cgatgaccgc gtctcctgtc tcaaacccgt
     3061 cgggtaaagg acgaaagctg atcattgggg gatcgccaac tgtcgaatga accggtcggt
     3121 gatctctttg tggatcaact cacggtacag gacgcggtcc attcgttcgg agtatttttt
     3181 agcgtcttgg tccgtgggaa agagaccgat ccgcagggag tacaacagat tcgttccgga
     3241 actcgagtag ccgtagacga aagccggaat cccttcgttg cgtaggatgc ccacttgagt
     3301 tttcaatttt tccagactgg agtcagcgga gatttggatg ttgtataggg tatagagcct
     3361 tcgtagttcc aatccctgaa ggtcttccgg tatttccggt atcgacggta ggaccgtcgc
     3421 cgttcctgtt cgagtggggg aagccgatag cgtgatcaat tgactcataa actcccggcg
     3481 aatcttctgc aggttgtcta actggatgac ccaagccggc tcgtaacgcc cgatcaacac
     3541 caagctgtat tcgttgtcgg agtaggcgga cagcaggtag gggagttggc tatcacggat
     3601 gaggcgtagg gcggcttgat cgttgcagat atagaaggtt aaaggcttgc cttcgtagat
     3661 cgtggtttgc gcgtttttga aaaagcgttc gaactcaaaa gactctttct tcgccatatc
     3721 cggattttct attcccgggg cgatgggggc ggtgaggtct gtgggcaagg gccggtagct
     3781 cgcgctatgg gttccactca gcgttgtttc atattcctgg gcgacggtct ccaggttttg
     3841 aacgcgggag ctgaggtttt gtatgattaa atttttgtcc tgtctttcgg tgagtaagcg
     3901 attaatccag aagcccatta aaacgacggc caacgcgagg gcgatcacga cgatcgccag
     3961 caactgcccc tgcagcgcgc tcaatccgat ccggttcgga gccttcggtt tttttggttg
     4021 tggtggagca ggaatcgtcg ggcccatata cccctttctg aactcggccc gttggttgac
     4081 tcggcttccg ccctgtttcc ggtgtatcgg ttgcagatta tccattttcc ctcaaccccc
     4141 ttatcagaaa agaaatcggc cttgctccga caagaatgaa aagaaagact ttcaaaaagg
     4201 cgcaccctcg cgtatcgggc gtccttatcc ctcgtttcta tctcccaaaa tagtattctt
     4261 tgagtatgtg ttcgatgatt gtggccgaga cagtcgtttc ctgagagagg ccaagccctt
     4321 ttatcacctg catcttgatc gatttcttct catcgatcaa agagaacagc gtttgcaaat
     4381 cgacctgtcg ctgcggaggg agcctgtcct ccaatccgat ctggctcaga gtctttcgga
     4441 tcatctcgag cgtgctttct tctatgatcc cgagcttgaa cgagatataa gcctccaaca
     4501 tcaacccttt gatgaacgcg tcgacaaaat ccatctgaaa accggtagcc gtctgtagca
     4561 agattcccat agactctccc gggatcagac ggtgctccaa ggaacacttg gctctcagaa
     4621 agtggtgctc ccttaacagt tcgtaaaaga ccgtccaatt cgtttggttg tggatcagta
     4681 ggctcaacga ccgttcgacc agataaaaaa aacgctcttc gaaccccaaa gcggtggcaa
     4741 aacagacgac atgcgctttt cgcaaatcct ccacggcgaa cgctttcagg aaaaacggga
     4801 tgatccacag ctcatgcggg ataccgaaga cgctcaactg gtttatctta cggtcgaaat
     4861 ggatgtagaa gctccgttga taaaacccgt acaactgcga aaaaggggtc gtcggaatcg
     4921 cccgcatctt taggtcctga aaagaggtcg ccagggcata cccaaccaag tcgatcaacg
     4981 cgccgccgcc tatgacattg atcatctgcg ctcgagggct tagagtctgc agaaccgtgt
     5041 agagttcgaa cacacggtcg atcgatttgc agtgctcgcc cgcgctcacc ggataggctt
     5101 tccgatagag agtctgcaag tccgcgatcg cagatttcac tttcgtgtca atgaccgtat
     5161 aggtgtgttc ttcgcttaag atccgatctt gaaatccgct ttcccgttct cctaaaaata
     5221 gaaagcagcg ggtttgaagc tctttcacaa ccgaataggt gaatatttct ttcatcgttt
     5281 caaaaccacg cggcctatgg cctttccggg aaaacggaca ttgatgctag tctaccaatc
     5341 ggactttaaa gccaccgcgt tgagtaatct gacggcgttc ttcacatcct ccaaatgaac
     5401 catttcgtgc ggtgaatgaa cgtatcggca aggaatggac aaggtaccgg atttgatgcc
     5461 ggcgcccgtc aattgatagc cgtgcgcgtt ggtgccccca tagatcaaga cttcgtactg
     5521 aaacgggatt tgatttgctt tcgcgcattt ggtcaacagg cgcacgacgc tttcgtcaga
     5581 aatggaggct ttatccttta tttttatcgc aggccctttg cccagttgca ggtccatgcg
     5641 cttattccct ttcggcgtat ccgtatggcc ggtcacgtcc acggcgatgg cgatatccgc
     5701 gtgtatatcg taggcggcga ctttcgcgcc cagtatcccc acctcttcct ggacggagaa
     5761 aattccgtaa atattgtgat acgggttctg cgtctctttg atcgcctgaa ccactatcgc
     5821 gcaagcgatc cggtcatcca tacttttaga gaccaacatc ggatccatat cgacaaatgg
     5881 cgagaggtac gagccaaatt gaccgatgtg cactttcttt tgagcgtcct cgcggctcgt
     5941 cgcaccgata tccacgaaca ggtaatcgta actcggttgg gtcaggctct ttttcgcgtc
     6001 ctcgtcggtt tctccctcat aggagacgat accggtgacg tcgtcgaaat tcagtagcgt
     6061 gcccagaagc atcagcggat tcacgccccc caccgggtcg atccttaaaa accctttttc
     6121 gtcgatattg gttatgacga cgccgatctc atcggcgtgc ccgtcgaaaa taaagttctt
     6181 tttattctcg ccgtttccat tcttccaaac gatcagattt gagacgttgt cgaaccgata
     6241 cccatcgata aacccctcaa gctcttcgag aatggcgagg tgcacttttc gctctctgcc
     6301 gctaggcgcg gatacttggg tcagcttttt gatcagctct ttcatacctt tacctccaag
     6361 gcttttagca ggatttcttc ggttaagatg tccgcaatga attgcttggt gttctcgaag
     6421 tcttttttag agatcaccga cacgggcgaa tggatatatc gcgaaggaat gttcacaccg
     6481 gcgcacggaa ctccaaaata ggtactggcc aaacgcgtgc catccgtacc gccgaccgtg
     6541 cgtcctttca attgatataa gatacctttt tgattcgcga aggcgacgat tgcctcgaac
     6601 aaccggtaat cgagcgcgta accgctttgc aggtaactga tcgcaggtcc gttgtccaaa
     6661 tgggtcgacc accgttcgcg tttgagctcg ggattgtcgc cggccgtggt cccttccacg
     6721 acgatcgccg cgtcgggctt gtagcgtttg agggtcgatc cgctacctcg aaggccgatc
     6781 tcctcttgaa ccacgaaggc gaaaaccaca cgatggttca gaacgggtaa ttcacggtac
     6841 aacgccatta agagcgcgca acccgcccga tcgtccaacg ctttcccgac gtatcggttt
     6901 tcgtactcct caaaggggct gtaaaaatgg acgtaatcgc ccaggttgac ggacttttgc
     6961 gcctcttcct ttttctgagc gccaatatcc acacggactt tgtcccaatc cggaagcgtt
     7021 ttgaagtctt ctttctgaag atggattgcc ttgtatccga tgacgcccgg gagattgttc
     7081 tctcccaccc aaacgcgttt ccccaataac accctcggat cgacgccccc gatcggagaa
     7141 accgccaaca ggccatcgtc ctgtatgttt gtcaccatca ggccgacctc atccgtatgc
     7201 gccatgagca agatcgtttt atctgacgtt ccgttcccct tcttctcgaa gaacaggtta
     7261 cccaaaacat cgacttgccc cggttcttcg gggcttttca gttgctcttg gatataggag
     7321 cgaacacggt cctcgttgcc actcgcgcca ttaatttcgc ataagtcttt tagaaacatc
     7381 ttagacgaca ccttccttca gtgccgaaac gaacaaggcg aggagtctcg cggtctcgtc
     7441 gatgtctttg acgcagattt gttcgtaagg cgtatgcata tagcgaagcg gtaaagaaac
     7501 gactcccgcc gcgaccgttt ccagctggat ctgatcggtg tcggtaccgg ttcgggaagg
     7561 cgatggctcg atttggtacg gaattccatt ttgcttggcc gtctcgatca ggagattgga
     7621 tattttgcga tgagggatcg ctcctacggc gatcaccggg cctttcccaa actcgttctt
     7681 tggatagttc ggagaggtgt cgatcgcgtg ggtcacgtcg agcgcgatac cgatatccgg
     7741 ttgtagcgcg taagcggctg tacgggcgcc gaaggcgccg atctcttcgc gggacgaaaa
     7801 agccgcgaac acgtcgaaat cgtgtcttag ggccgtcagc ctctccagca tcttgacgat
     7861 gatgacaccg caagcccggt cgtctagaga cttgccgaag aaaaaatctc ctgtggaaaa
     7921 gggttcgttg tcatatgtga cgaaatcgcc cacggagatc tcagaaaagt cggacatcga
     7981 aacgtccacg aataaatcct cgaacccgtt caatttcttt ctggtctctt ccgtctgcaa
     8041 atgcggggcc aacatcccga taacgcctcg caaccgtttg cccgatcgcg tatggatcca
     8101 gactttctga gaagggagaa gcttgacatc cgcgccgatg ccttccaagc gcagaaaccc
     8161 ctccggaaga actttggaga caagaaaaca cacttcgtcc atatgggccg aaaaaaacac
     8221 ggtccctttg tgttggccgt atctcttaac gatgaggttt ccactggtgt cttcaaaaac
     8281 ctcatccacc agcccgttca catacccttt gaaaagcccg cgaacctctt tctctctacc
     8341 cggagggccg taagccaaag ataagctttt taaagccgcg aacgtatcca aactcaactt
     8401 atctcaccct tcctattttc ccgaaaatgt tcggagtctc ttgtttctct atcggtatct
     8461 gatgtattct tccgttttta gccacgcaga cgacgcagac ctcgctcacc aaagagtagg
     8521 aaaagacctc gccgattctc aagtgagcgt tcactaaccc gagcgcggag atgaaagagt
     8581 tctcggggtc agccttcccc gcgtgcacgg tcccgtggat tttcccgtac acgatcacgc
     8641 ttcccgctgc tttcaactcg gaaccgtgat gtatattccc cataacgaaa acgtggccgt
     8701 catagtcctc agactgtccg gaccgcaacg acccctctac gacttttagg ggtttgagtg
     8761 tcgaagtttc aagtgggaag ggttcttggg aagtgatcgg ctttcgcgcg gtcggtgtca
     8821 ggttggcccc gggatgacgg gcgagaggtt tttcaaagga ataggctttg actgaaagcc
     8881 ccgcttggcg cagtcgcgac ccgatcaact gaatctgccc aggtttctcg gtttcgtcct
     8941 caaaccagac cgtcaccttc gacgccgtat tgttgaaatc gctcaataac ccgatttttt
     9001 cttcaaggaa agcctccaac tcgtctatct cagtattcga tccgacaaac aagatgaggt
     9061 cttccccgtc gaaacgcaaa tccaccataa tgtccctctt ccttcgtgtt atctatgatt
     9121 ataacacaat caaatcgaag aacgtccgat attattcccg caccacgcgt cgcttccgac
     9181 tccgaatatg gtagaatacc gtcggtgaga gaaggaaaaa tcaatgccgg ccggaggacg
     9241 cgatgaaagc acatttcata gggattagcg ggatcggaat gagcgccgcg gccttgttgg
     9301 cgaaggactt gggatacacc gtatccggca gcgccgatgc ggaaaatgat cggactgagc
     9361 ttttaaggga ccgcgggatc gatatcttca tcgggcatcg cgcggacaat gtgcaaaggc
     9421 cggatctcgt tgttttcacg aatgccgtct ccgagcataa cgaagagttg ataaccgtac
     9481 gcgcgctaag cattcctgcc ttgccgcgat tgacctttct cggaagcgtc ctgccccgat
     9541 atccgaaacc ggtcatcggt atcaccggaa cggacgggaa gagtacgacg acggcgatgg
     9601 tggcgcatat gctcttagaa aatggagagg atcccaccgt gttgctcggc gggctgcatc
     9661 aggacttgga agaaggaaat taccggcggg gcaagggccc tttggtgatg gaagtggatg
     9721 aaagcgacgg gttcttgcga gatttttcgg tagacgtcgc ctgcttcacc aatatccacg
     9781 gcgaccactt ggagcattac gaaaaggatt actcccacta tcgagacgcc atcttcgatt
     9841 tcgtctgcca tgcgcgcgag cgggtctttc cagccagtta cgccgatgct tatcgctcct
     9901 actgcaattc ccgaggaata acacccggga agacgcatct cttcgaactc gacccgcaga
     9961 tcgagcgctg gtatcatcac acgataccgg gtatacacaa tcgtttgaac gccagcgcgg
    10021 ccttgaaggt gtgcgaggtc tatgggcttt cgcgcgaacg agcgatcggc tgcttagaaa
    10081 actttccttt cgtggatcgg cgtttttcga ttcgatacga gtcaaaccgt gtgatcgtca
    10141 tcgatgatta cgcacatacc ccaagagaaa tagaatccgt tctgcaagcg gttaacgaac
    10201 gttttccaga gtcgcttccg cacctcgttt ttgaagctca ccgctattct cggctcgaac
    10261 gagaatttca cgacttcgtc gccgtcttaa gctcccgtca gatccaaaaa acccatatct
    10321 taccgatcta ttccgcctat gaggaaacaa aacccgagct cttcgagcgg tttctcgtcg
    10381 ctatgcgcca aaaccggcca gacactgtct tcatagagga cgccacccag atcgttccca
    10441 caataccggt ctcgccgaca gcacccgtcg tctacttgtt tgtcggcgca ggccacagct
    10501 ccctccactc gaaagccttg gcctcgtatt tagagcggca ccacccgtag gaagcgcggt
    10561 tttgcgcgca tcgctagtgt gtgtgggtat gaccttcgat gtcgttcacg ggttcttcaa
    10621 tcgtctccat tttcagcagt gccttcgaac tctttcgcga atacgcgttc atgcggtgaa
    10681 gtaaatagcg cgcgtacagg atctggaaag gcagccacca ggtgtggccg gtcgggagga
    10741 tatagcggtt ggcgcccttc atctcgcggt acaggagttg ccgactcgtc cggggtaggg
    10801 ttttgtcgaa gaaggcttcg atgaaggtca cccggttttt atccaacaga tgcgcgtagg
    10861 agatcggatc gatacgaaag agcgggtgaa tcgcttcgct ggtgagcagg gtttctaaat
    10921 ccgctttttt cacgaacccg tactgttgtt ggtagataga gaagagggcc ttgggatcgt
    10981 gaagggagta gtctgtctgg taaccctcgc gaaacagctt tctggcgaac ctcgtcgcgc
    11041 tggactcgta gagtatttta ggaaaatagc ctccggtcgc gatgatcacc gtctggtgga
    11101 tgcgttggtc aaggactggg accatcgatg ccaacatccc gcccaggcag tagccgctga
    11161 tgcaattgtt ctccaaccac agcccctctt gctcgaggaa gtcgatcgtc gtttggatgt
    11221 ccacgatggc gtgttcccaa aatcggtaca tcacggatat gtcgggccaa acataacttc
    11281 gtccgctcat agagcctttt tctacgcggg tgtagttgcc gggcagaatc agaacctccg
    11341 cgttcatacc ggccgccgcc aaatgcatgc ccatccatag aaggaatttg atgttcccac
    11401 ttcccaatcc gtgtaggatg aggatcgtcg ccttcgcctt cgttttaggg tggaaatgat
    11461 agaggctgat cgtttccgtt cccggagcgg ggttgcgata tagcgtatcg taccgtatgt
    11521 agctgcagcg gtagttgatc cccttgaaaa tgtacccgga gaggaagcgg atgggctgct
    11581 ttttgtagga aaaatcaatt ttcatagtgc tttctcagct cctcgttcag aagagaaggg
    11641 gttggcttcc gatctttcag atacgcccgt aaaaaccgga cggtttgttc caaaatccaa
    11701 tcggttttga cgtctttttg agagaattcg aaggtatgat taccagtttg atggacaaga
    11761 aaacgaaagg gtaccttcaa gcgttttagc tcgcgggcga actgcacgct cgagctgaac
    11821 ggaaccgtgg tgtcttcctt cccgtgaacc agaagggtcg gccgcatccg ctcgctgagc
    11881 caatagatcg gtgagtattc ttggtagagc tcctcgatag agccgggcct tccccgcaag
    11941 gttaataacg tcgcgaaccg ggcgaagaag gatttactgt ccttttcaaa caggtcccgc
    12001 aaattcgtcg gggcgtaaaa ggcgatgacg cctcgtattc cgctcagctg ctcccgtacg
    12061 ttatggaagg tttggtaggc cgtgtacaat aaagccagat gccctccggc agatagcccc
    12121 atcagaaaaa ggtgatcggg gtcgaccctc aactcgtcgg cgtgttcgcg cgtatacgcg
    12181 attgcgtctg aaaaatcgga catgatctca tcgatcgatt tcaggatccc taaccgatag
    12241 ccgacggata cggcgcaaaa tcctttcgaa gccagataac ggcaccaaga cacgttgttc
    12301 ggctggttcc tgaatcccga aatccatccg ccgccgtgcg cgtagatcac ggtcgggtac
    12361 gccgcgcgtc cattttctgg ataccataga tccaatttca aatccccgac gtcggttttc
    12421 ttatagacca ccacgcgctt ctcaaactcc ttcaatgggt ccaaaggtac tttctgagtg
    12481 agtatccgga tgagaaacaa gaatgagaag acgagaaagc cgacgatcgc gcttgaaaaa
    12541 tcgaacacca gaaaatagag aaacggtatg agcaggtata gtttgttgac aaagaaccac
    12601 gattttttga aggctttgat cgctgttttc agcatactgg cctattatat caaataggaa
    12661 gcctttatta tttggagggc gtttttgtag attcaaatga ttgacattta tataagaacg
    12721 tgtataatat ggcgaaaata ctcaggactc ccccgataaa cataagagta ttgggaataa
    12781 tcgcctatat ttacggataa acgataactg gtggatttaa cttggctgag aagaggaaag
    12841 gactgatcgt tggtggggcg gcgcaagcga ggccggcctc attgggtagc gaattggcaa
    12901 tccgcgcgat ctcgagtttt tactccgaga tagggttgtt gggcctttgg gggtcatgtc
    12961 cggatcggtc tttttggggg atcccgctgc tcgggctgtc cgcggaggtg tttttccggg
    13021 cgcccgatac caagcccctc acgggtattt gcctaggacc taacgatcgt attctctcga
    13081 gcgcgctccg cgagaggtgg tccttcatcg ggctaacgga ctgtcacgga atcggtcttc
    13141 gaggtttcgc ctacgaactc ggctaagaat cggtgttccg agatctcacg cgaacgctca
    13201 gtaaccgggc ggtggctgaa gaagtcgaag tcatcatcga gaatggggat ctgctctatt
    13261 ttcatctgtt ggacgcgggt caaaacgcgt agaaaagtcg tctcgttaag ggagggatag
    13321 gcatatgtcg tcaaaagaac tagagaacaa atcggtggag gaattaaagg aaatggcgaa
    13381 aaaggtgaag gtcaaagggt actacaaaat gagaaagtcc gaactggtcg aagcgctcag
    13441 cgaagagtgg acgggcagcg tggacgagga gaccagggtt tcgattttgg ccgagatcga
    13501 aaaggacccg aagatttacg agctgcgcaa tatggctaaa cggttgggga ttacgtttca
    13561 aaggaacgcg aggaaagagg agctcaaaga acgcattctc cggctattgg ccagaccgaa
    13621 gttggagatc aagcgatacg agcgctcctc ggggccggcg cctgagaaaa tcgttccggt
    13681 atcgacctcg agtacggtgg atctccccac tacgtatgac aaagataccc tgcaaggtct
    13741 tccggtcaac ccgtcctggc tctccttcta ctgggatttt tccaagcaga cggacggtct
    13801 ggtcgatcgg ttcgctcgtg aaaacagacc gctcctgtta cgtgtgtacg acgtcacctt
    13861 cctgattttt aacggatcga acgcccatcg gacgtgggag tatcgtatta acggcaaaga
    13921 gaaaaaatat tacgtacagg tggctttgcc ggcggccagc tatctggcgg agattgggta
    13981 ctacaatgat accggcgtct tcgttccctt gcttcggagc aatctggtga acacccctgc
    14041 cgctagcctc tcaaatcgcc aggaggagag gtggatcgat cttcgaaaac atctgcgctt
    14101 ctcgcagacc catatctccc tggcttctcg cccaaacctg cttcctaccg gcctttcttc
    14161 tctcacgacc gaagaattgc gcggtagttt ctcgtctatt ctgttctcat ctcgagaagt
    14221 acaaaagaga acctcggacg atcggtcgaa agaataggag gaaaaccgta tgacgaaagg
    14281 ttatcttaca ctggtattgc acgcccacct accctacgtg cgccatccag agttcgaaga
    14341 atttatggaa gaacgctggt tgttcgaggc aatgacggag acctatattc ctttgctcag
    14401 aggtttcgaa gcgttggaga cggatggcgt gccctttcac ctcacaatgt ctctcactcc
    14461 ccctttgatg gagatgctgg aagcaaaaga cttacaggag aagtatatcc gccatctcga
    14521 gaagctaatc gaactcgctc agaaagaggt cgaacagacc cgcgcggagc accccacgaa
    14581 acacaagatg gcgcaatact atctgaagga ctttcaagaa aagctcacgt actttcgaga
    14641 ccgcgtaggc tgtcgtttac tcgaaagctt caagcgcttc caggacaacg ggtctttgga
    14701 aattatcacg tgcaacgcca ctcacgggtt tctccccctg atgagccgat acccccaagc
    14761 ggtaaaagcg cagatcagtc aaggagttaa aacctatgaa aaatacttag gccggaaacc
    14821 ctcggggatt tggctggccg aatgcggcta cgtgaaagga atcgaaaagc ctttggcgga
    14881 cgaggggctc tcctactttt tcgtggattc ccatgggctg tggtacgcag agaacccccc
    14941 gcgctatggc gtttaccgtc ccgtcttgac ggaatcgggc gtcttcgttt tcggccggga
    15001 tcccgaatcc agcgaacagg tttggagcgg tgagatcgga taccccggtg atttccgcta
    15061 tcgagagttt taccgtgacg tggggtatga tcgcgaattg gagtacatcg cctcgtacat
    15121 cgatcggagt ggcgcgcggg tcaacaccgg catcaaatac caccgagtca ccgggaaaaa
    15181 cgtggacctt gggaaaaaag actactacga tgtcgaagaa gcccaccggg tggcctatga
    15241 acacgccaaa gacttcatcg cgaaaaagag cgcgcaggcg gaggccctat acgaccagtt
    15301 cgcgggtgtt cagccgatca tcgtcgcccc ctttgatgcc gagctcttcg ggcattggtg
    15361 gttcgagggg ccgaagttca tagaaaacct cttccggtgt atgaacgaaa ccgaggtcat
    15421 cgcgcctatc tctccgaaag acttcatcga cgatgtcctc accagtgttc agatcacgac
    15481 gcctgcggat tccacttggg gggcgaacgg ctacaacgaa gtatggctca atggaagcaa
    15541 tgactggata tatccgcatc tggacgagat ggccgaacga atgacgcggt tggccaacga
    15601 cttctataac gaggttgatc cggtcaaaat acgcgcgctg cggcaagcga tgcgagagct
    15661 attgttggcg caatcgagtg attgggcctt catcatgtcg accggcacga cggtggagta
    15721 tgcggtcaac cggaccaaac aacacatctt tcgcttcaac cagctgtacg aggcgctcta
    15781 taaaggtgag atcgacgaag cgtgtctagg gtactacgag tggatcgacg ggatcttttc
    15841 cgatcctgac taccgtcttt acgccctaag atgagcgggg ggcttcttga caacgggctc
    15901 aaaatatgat atatttcgtt gctgctttgg aaagccccag tagttcaatg gatagaacgg
    15961 tggattccta atccgcagat gtaggttcga ttcctacccg gggtaccaaa tcaggatgcg
    16021 gggacgtggt gcagttggtt agcatgccgg tctgtcacac cggtggccgc gagttcgagt
    16081 ctcgtcgtcc ccgccatctt tttgtctcac gaggttggct tccgttcgtt gccccgtgtc
    16141 ttcattcgaa acaagattat ttttttcctt ttgtgatata attataatca ctacccaata
    16201 tctcaccttt tcacatctcg ttaacttttt ataacttatg aggtgatgcc gtgaagcttt
    16261 tcttggatac cgctaatctg cagcagatac aaaaggcaaa agactgggga attttagacg
    16321 gggttacgac caacccctct ctcatcgcca aagagggggt acgcgacttc cacggacata
    16381 tccgtcgaat ctgcgagatc gtagagggcc cggtgagcgc ggaagtgatt gcgctagacg
    16441 tcgaagggat gctcaaagag gccagggagc tggcgaaggt acacgaagac gtcgtcgtga
    16501 aaatacctgt gacaccggcg gggatccaag ccgtcggggt cctgtcgaga gaaaagatcc
    16561 gcacgaacgt tacattggtc ttttctgccg ggcaggcctt attggcgatg aaagcgggcg
    16621 cgacttacat cagccccttt ctgggtcgtg tagacgacat cggttgggaa tcctcggatc
    16681 tattggaaga cctgctcacc ctgattgacg cctatggttt cgacactgaa gtgattgcgg
    16741 ccagcgtcag gcacgcaaag cacgtcgttg aagccgccag gatgggatgc catatcgcga
    16801 caatccccta tcaggtcctt gaagcgctct ttcaccatcc gcaaacagac aacggaatcg
    16861 atcgcttcat caaggattgg aatcagctac aggatagact gagaaaggaa ggataacagt
    16921 gccgagaaaa ccgaaaggga acgagggaga gcttgctcct aaagctaacg aagcggctcc
    16981 gaatacggaa gcttcagaga tggagtcttc gaaaccggag aaacaagccc cgtcacccag
    17041 aacgagaaaa accacccgaa aaaccgctaa agacgagaac aaggtagata ccgtccaagc
    17101 ggaagtacaa acgccggctc cagaagatac gggaaaaccc acagacaaga aaccaaaggg
    17161 tagacctaaa aagaatgctt cgcggtcctc cctcttttcc tcgaaggaat cggcgccaaa
    17221 aaatgaagag ggggtaagcg ccgcgcccga agaagctcac gttcaaacga ttcaggatac
    17281 catcgccttc gaaccggagg aggaaacggt ggccgtggag cccttggaga gcgtggcaac
    17341 cgacgagcag ttgaggacaa cgatccatgt ggaaccggaa gaaccagcag tctctgtccc
    17401 ggaaaaacag cgggaagaag aggtggaagc ggttccaaaa ggagaaccgg atcgaaaaga
    17461 caatatccgg gaaatggagg tcgatatccg gaagctcaac aatatgaaca tcaaagacct
    17521 ctatacgctc gcgaaaaagt atagtatcaa cggctacacc ggaatgcgta agaatgatct
    17581 cgtcttctcc attcttcaag cccaggcaaa gagttttggc tattttttca atgaaggtgt
    17641 gctggatatc ataccggtgg aagggtacgg gtttttaaga accggtaacc tacttccggg
    17701 caacaacgaa gacatctacg tctcacagtc tcagatccgc aagttcaacc tcaatgcggg
    17761 cgatatcgta tccggtcagg ttcgggcgcc taaagagggc gaaaagttct tcgctctgtt
    17821 gcgtatcgag gcgatcaacc ataacccgcc cgatcggatc tacgaccgga tcaactttga
    17881 gaacctgact gcgatctatc cggtgaacaa atttaagata gaaaacgatc cccaaaagct
    17941 ttcggccagg atcatcgacc tgttctgtcc gatcgggaaa ggacagaggg ggctgatcgt
    18001 ctctcccccg aaggcaggaa agacgactat cctgaaggat atcgccaacg gcatcgccaa
    18061 gaaccatccg gagacgctcc gtatcgtttt actcatcgac gagcgtcctg aagaggtgac
    18121 ggatatcacc cgttcggtcg acgcgcaagt tctcgcggcg cccttcgaca tgcccccgga
    18181 aaaacagatc aaggtggcgc agttaaccct acagatggcc aaacgattgg ccgaatacaa
    18241 gtacgacgtc gtcatcttgc tcgacagcat cacccgattg gcgcgcgcat acaacaacta
    18301 catcccgcct agcgggaaac tcctctcggg aggcgtcgac ccgaaagcgc tttacggccc
    18361 gaagtacttc ttcggagccg ctcgtaacat aga
//
LOCUS       DAOPXN010000025        18374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3082_length_18374_cov_5.732846, whole genome shotgun sequence.
ACCESSION   DAOPXN010000025 DAOPXN010000000
VERSION     DAOPXN010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 18374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18374
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3082_length_18374_cov_5.732846"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..3275
                     /locus_tag="PKM49_02555"
     CDS             636..3275
                     /locus_tag="PKM49_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81728.1"
                     /translation="MERKFFGLVAALLVFLTGVLFAVNAPVQDVYNFDVRQSLDGLTG
                     LKPHVKTELVELIQSAIAKNLDPSIEIPNFGKRLQVAWEPETPITKLELYVMAVKALG
                     REDMALKLYPMVSPTFADIQTGLERDPFAVQEFLYQRAYGYAEYYQNFLLKEFPILMN
                     FVPEPKSSLDITGIQIKQDIRIDGTGTIVYTTPWTQAAADPKANNAGVATLQRTQATR
                     AEVIEFIVRLAQVKYPELFSSVSLEADVTKALDLENKGIVAVSEALSKHLRTLYGQSY
                     PENVGFVYAFEFFFLSEMDTAKDGKVAAPLLYLDTFRSRIESGKNVVLNENYGANRTV
                     LAANPAERFWAVALFAKVFAAEKSKVESNMKAPERIINNVIAFKPVGVFKGQVMFNKV
                     GCVEGEVSSILCAGCKDAALGAAASDIRATFLKLKDGKYYVLTDMTVWDSNNGYPNGI
                     QFANTYCADDGVNTENPCEVGIAHPQSVTTEKSFTLEAIGFGTIADNKSRMVNVDKIW
                     IPLKQQEAYEKVKNVYGLELKDITAKYPKKIESGAIDLYRLFFPNIEDITQKGRGLEK
                     AVDMIVYGKQGIYDFDLYVKRAANAVAVDNLLKGEKVVFNISFNVNPLDGGVALKKVE
                     LLPAIDRLNHRTEVLTLKGVKDFVDLYAGLIDTISTDPNQPMVAYYKKLVEFAKEHNI
                     PDSAQLVAIIKTPTVDREVQSTTTQFKMMELVLGQLVQLELKPGTDAKLCDLENANAF
                     DPGSSAAVSTCFGEAQDEYAINKLNQTICEKEAEAKKVFRFGDIVEDCCVTLAKGYWA
                     VKVDPYLATFLFHENTKVALFNDLGLVSVGNVDLLRLDFLGFKGPIVREAVLVKPLDP
                     LNLFPARMAESKEEIYVKAYEVK"
     gene            3364..>5516
                     /locus_tag="PKM49_02560"
     CDS             3364..>5516
                     /locus_tag="PKM49_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81729.1"
                     /translation="MSKKKKWLIVSLSIVVVCILVLLLFPVKGIFRVTVETAPSQRPN
                     LETFLKSLFISIKDLKGQAIHREVLGVERYDSIPLSKGTYRIEIETLDGVNLLSEELT
                     IPWGNLCFKPRTQEVRFTVPPAVEIRPENVSFALKEEKLWIRWKNSGWSKKATFQVSV
                     TDFGTLTTENNELYFPIESNALTQLDALNVRIRVLSGNQYQTLYSGDLPVKHFKKTET
                     KALSAEQLEVSWEHEKGTFTPTGYRILVEGREYASVGPSATSETLDLETLNPQPNEGK
                     RREINLTLEAISDGIVLSRSDSVFYMYETVFSSTLNDEWRTVNETAPLKVFINERPVA
                     LRLEGETIKAFYLSFLPEETLTVEYTVNDYSILRRTMPLREERETEAITLPSLSRVFS
                     AQAEVTEDERLYLQWSADRSEGKINPTRYYVETPQGTQTTTENALHIPYESKKQEIKL
                     FSVFFGTVSFLEKVIIKPEKPGLTLIRIDYDHKEDVIVVEGRASITTPFTVLYRIDRY
                     APEGGGWPEERAGLIYEVSQKEWIVAEDVITSEAGEFSFWIPVKDLQALPPLEEPLKI
                     NGSIKVLDTFNQSAVKDFEVVRFLPPKVEFVRFLINDEKLLLEWKAEDPVLSQRYRDF
                     AVNVTTSTGESYRYEATQTSLEIDLSALKDYRLTLTLEALWENEIVRLDDRSFEFLPL
                     DFNRLVDWNLGKSGKETWKIVYYVKYQKGFESISF"
     assembly_gap    5517..5526
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5527..5759
                     /locus_tag="PKM49_02565"
     CDS             <5527..5759
                     /locus_tag="PKM49_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81730.1"
                     /translation="ITGSFEWPIKSGKTYLIRAGIKYDIETNKDLIAQTVTTEVYETI
                     PPSTGGIKEAYQIAKDKIRLEAIDSAPGMEYY"
     gene            6039..7019
                     /locus_tag="PKM49_02570"
     CDS             6039..7019
                     /locus_tag="PKM49_02570"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81731.1"
                     /translation="MRITQSRLKFPPKTSLRIEMGTLYLEEDVRLNVMENENNELWEG
                     VYIFGGRVYVKRAIEVSNAAIAFQLYSSEGSIEDTSFLNCQKGILAQGSKLKVSGNSR
                     FTTIGLAISARNSEVTVENTFFRNVQEGFLFLNNKLIRIYQVNITGVEKRGAYIATSK
                     DVELSNVTIDGIARDQQEGFFIEDSVVRMRNITLKNLKTGLIFVNTDASLDSIIVYQV
                     TDGIHLEYAYFELNNSTVEADNLGVFAITSPILRTREFSGFLPTTGKVGSFAGLIRQT
                     TVKAPVAVKIERGENHIAVENSQLIGEIIHGGIQVNPNGLPYYRGEIIQK"
     gene            7101..9116
                     /locus_tag="PKM49_02575"
     CDS             7101..9116
                     /locus_tag="PKM49_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HNY81732.1"
                     /translation="MLIYVLTSGYGALKVQKVDVAFYPYVTLSVSGEPLTSDQVCLYE
                     NNVQIEPIVDFTSYDMRSQKPLDLVFVYDFSWSMRGYLESFQSGLSGFIAELKNLGYD
                     TRLSWVGFAAEVLHIYPNTQGCDPPPEFTSDLDAFEDYLSKVMTEGKIPIGFEECQIQ
                     ALYEASKFNFRENAVKLIVLFTDEDTQDDLEGNLYYLPDLKEQIDEKGLVIVPVFSQE
                     EPDPIYDELALFSGGKSYPFIPNEVISDRGFAFGAYFWEFLRSLAVEQNTFARITYYS
                     PGKFGERTGALRHCTTQSEAKFSYHIPETANKIQVFGVDTRDSPIVTLFFSPGLGEAT
                     EGYQAEENGENIDFARSVHITPYQPDRVDLVLIVDLANSSISYPAQIQEHVATLIQYC
                     EDIGISLRLGAVIPLDQQILYQDLTENLDQVANLFNRYLKVKTVTNTAAIPEMFHKAL
                     NYHYRANSQKAIVFITDAFQNELRKGFESGGPLIDAIVATSLKNNIGLFFISQKNDYI
                     DYINRMTPGTWIGFGEKPTHQYLNGFFEDFFRQQQALTYESRAFYQFCYDPSFFTLSK
                     DGKRLFIQFFQFSDLSYQTRLQINTVTSSPAILGRGETTLLTCHAYPPKNIFYYWECA
                     DGSFVSPNGVSEITWKAPEKIGPHNIYVEVSLGQIKMKGSVVVTVQP"
     gene            9130..10326
                     /locus_tag="PKM49_02580"
     CDS             9130..10326
                     /locus_tag="PKM49_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81733.1"
                     /translation="MSKAALLLIVCLGFTALGFTGGYIKGIELQGFPIIRFHMTLEGK
                     IGDAAMTLYEDDREVKIEYVEVKSLRESPSADIVFLIDNSRSISGFETLIREKSSELL
                     QLLIQNKIDFTIAVIGFSDVINTVSFPKTSAASSLSHLNPFTSDPYLILETVSRAVLF
                     NGNHPSKPFEMIAMAAGMPFREKATKMIILFSDECPVESLINPELYRALGPFLAEKKI
                     SLSIFYDFHRQSFYWDYYKRLAESSGGHFLDLNESLLFLPVLAGLSEEKMELWVWFRS
                     QQTAFDTKVHRVQFKDRNKTPLFAPYDYRFPEEWTYLRLNTITATPFMVERGAPVFVK
                     VCYYPYSEDTRIVFDTGQGFTLVERYEEAEALLLAPDKPGSYRIPVTVSRGKESLDGC
                     VVVHVN"
     gene            10349..12133
                     /locus_tag="PKM49_02585"
     CDS             10349..12133
                     /locus_tag="PKM49_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81734.1"
                     /translation="MRKNTLRLFAFALLLFCGPPLFSGELLFTDINTFLQCPVVRLLN
                     DYTVPVQAYLSDFGDTYSQTTTKRALGWFGPVKTVTQESAEIRRKFGEDTEVTRTTEL
                     VVRFNEYGEIVDERIYVPFGLDMLRLDRSFIKKPLDEPLDLPDGVHFLLIQRETNLYG
                     QVVVERMIDWNGDLVYEIKNRYSETNAILEKAFYTSAGFVSARTFSYDDIGHRLISIR
                     ERDVWGRTTYEILLGYARDDRGEYYLQSEERVNDIGHTEYTRLTDPDGRLLEEAIYDR
                     YGQLYTKKLYQYEADRLVFSEAHTAEGLYNRQKWTYKAGLLVEHISSDALRILERDTF
                     RYNATGELVEQNHYGVSGVFLWKRVYQPHASRPSEITQYTSQGDVFSKQLSLYDVKGR
                     LISVKVYSSYIDKVARVYFLESEETWEYIENATKKYVEKTIRRLVIFDRSGKKLSDLE
                     VERQFDERGNVLEEKTKLSGKEVASFKRKFDEQNRVIEEEVKNTLENYKRQLSFHASG
                     QPKEIIYLEGTKKSKVFVYDEGNNLITEERYDANGSLIYKVEYERFFDAWGNWIKVIR
                     YDWEKEPWEDSLKKTPKFVVYRNFEYYE"
     gene            12158..15976
                     /locus_tag="PKM49_02590"
     CDS             12158..15976
                     /locus_tag="PKM49_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY81735.1"
                     /translation="MKKLSIFWFVICLAALCFALTPQELFDRVDKEPVSRLSSSLEKL
                     SPEDLKTLLALRNAEGDSFLNAIVRLKAEPIALYLLKNGADAFEVAAGETAFSLACVL
                     DLWDFAISAIEGTKSYGENQPILWEGVFEAVKNDNHRLARVILEKTIEYRANASIVSQ
                     EALIDAIKAQDVFLALDLLRSGANLNAVDRTGSTPLIIAIANGDNRNAAPLIFLGADV
                     DIQNHRGDTAGMLAVKTDNLEALVWLFEKNADLSLKNSDNKDMMDFAIANGNEEIISA
                     LLTEAIDQKRYYILSDWVARLEEQRDQKADSIALKMAADRKKLLNELAAALIEYDNAL
                     IKDPQNALAVLGRADVFFLMKLYEAAFEEYQRANGLYSFYSEAWLGLAKTRMHLADMN
                     PETEQRKPAYEQAREEAKRALEINVAYTEAYYYHGLILVRLSAFQEGDSRKKTLEYAV
                     EQFDKALALNASYTPAFEAKGDALRLLGEFEKALVAYAAYLKIDPERLEIIEKRGDTY
                     VEMAETAKAKSKPNDEIANYKSAITEFEKVVRVDADNERVWKKVGNSYGKLQDIAIDA
                     ARKAQLNGEYKTQDEMEASAKGYAEKASRAYEKYLEVLEKKKLEDDLEIREKLGFSLF
                     YRGSYSEALRVFAEILKEYADRYASLFGKARCHFELQEYEMALQVFSQLAGYPEYDTY
                     TAFLHAETLFALERYAEAKAVYEKAVALRDQKEKGLKIGEIWYQLAFIASLEGRYKDS
                     IDLVNKAIQDNPTFAKAYFFKGEELFKHEEYAEALLALIKAVSLDGSYAEKVQWESKA
                     AHVDGVFLALKNGKDYSRAYPFLKTAIGYDDEARKYDLRSLIALTRYSRHSLDETAYL
                     WEVGDSANGLKRVQTLSDVLKTAVSERTFKSRSEVIVPYVQSVYMETQISKKMTASDT
                     SRSPLLKTDARIADCLRLCTDLLKDQNRNAAGWMALGLVYELREQVPLAAVCLNNAYG
                     IEPTNALFLLLLSDYLVKSAHLEESRVLLSSLPEDFSWDVGLWFEPFARAERWRLLAE
                     IAVRQKNIPEAIDCYRYAIESAERFYIEYRMEGALRESDTFYTLQKTIADKAEQLRVK
                     KEYSLAATLLETGLSIQRFVNPAVRGKLELLAGKTAFTLRDLSGAEKLLKSAQADLPK
                     NSAPYAVLGYVYLERNEPRKALRELEKALTLAQNEGSDTSPILTGLALAYFALYLEEK
                     TESQLSSAFFYTNRAYAAAPQNVRNLVFKGDILLSENFKDLAASVYCLALQYLPEHST
                     IFEKLTQIKK"
     gene            16528..>18374
                     /locus_tag="PKM49_02595"
     CDS             16528..>18374
                     /locus_tag="PKM49_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81736.1"
                     /translation="MKKPIVVFVLLSVSLIMMALNLPAEETLSEISIEEGYVFQLRVS
                     SSDIAKEYFLNVLKNSEKKVRIALPYEATGITTVVLKPDGYKFSGKVRFCDGGEEFPF
                     EYRTNSYGMYVSSSYPGLQILPVYPLNITKTVIGSPTRFEWKLLSNQIVHGYDFWLGT
                     NRDALHLIASQIANPYYEMNLENLRFSGALYWKVEGYTRSEERFTSPVFSFTYQPSAM
                     MQAIWDAESEKYDKFLEKAEEILDRMKEISDSSDKISKLETEIATLTSASLSVAGKID
                     TVIGVVSDLSTELSALSTLVEEKESKTEDASGTILSELKAGFSTMTTIASDTLSIAKE
                     LRAVVVPSLETLDTISSDVTSCVAEIQKASLFWTPQQKTEILAILADYATPPTIEQNT
                     VVDLFYELLDSLTLLKKVALLKRLSETHGESWLFTGVEGFTRFVQVLFSGELWNAESI
                     PFVFEPYSKALDDLLDFYISKMKKTPTGQLLIDPLYLLPQAKKLLGFEGAKHFFSHYS
                     IEYYTYHQTLRNNETIKGIISKFWNGNGSEYKILLLLNYLIHEYTMKKNDPSLSQKIN
                     GDEFLSLYRSMNPDIEKFFNNERNKNYGTSYCYTLGWWELVWADIWTPDP"
BASE COUNT         4981 a         4358 c         4664 g         4361 t
ORIGIN      
        1 aaaacctctt attatacaat aaaagtgagg ctaaaaatta cggaggtgtg ggtataatat
       61 cggggtacac caaaatgtga acggggaact ttggttcggc gttcacaaga accttttcac
      121 cgtgttctct gtgccctccg cgcgaatccc tgtttctaag ggtaggtaca aaggcgtatc
      181 gcgccgtcgc gagaagaaga agcgcttacg ccgcgatgac tatatagact aaaaatcgcg
      241 cgatcgattc cggccttgtt tcctgattac aacccataac ttgacctatg taaaagccct
      301 ggggcctcag gtccgctttt gaaagccgaa caaaagcgtg ccgacttgag gttttttgct
      361 ctgcctggcg ctccacgacc tcttttcggt ctttatgggt tatgcggata agggagagga
      421 aagaaaggta tcgctcgact aaacgcgctc tcctaaggga aaaacctatc tcagttctcc
      481 taataatttg gttgacagta atcgggaagt tgtgatataa tctcccgcaa acgtatttgt
      541 ttcttgggac aatccagatg gcgcaagtgt cgaatgcagc gagagaccat aacagaaata
      601 caatattaca aactctagag agggggagaa aagttatgga aagaaagttt tttggtttag
      661 tcgcagcact attagtcttc ttaacaggcg tcttgtttgc ggtcaatgct cctgttcagg
      721 atgtttacaa ctttgatgtc agacaatcct tggatgggtt gaccggactt aagccccatg
      781 ttaagacaga gctcgttgaa ctgattcagt cagcaattgc aaagaacctg gatcccagca
      841 tcgagatacc gaacttcgga aagcgactgc aagtcgcgtg ggaacctgag acgccgatca
      901 caaaattaga actctacgtt atggccgtca aagcgcttgg cagggaggat atggcgttga
      961 aactctaccc gatggtatct cccaccttcg cggatattca gaccgggttg gaaagagacc
     1021 cgtttgcggt ccaagaattc ttgtaccaaa gggcctacgg gtatgccgag tactatcaaa
     1081 acttcctgtt gaaggaattc ccgatactga tgaacttcgt gccagaaccg aaatcctctt
     1141 tagatatcac ggggattcag atcaagcaag acatcaggat cgatggcacg ggcacgatcg
     1201 tttacaccac accttggacg caagcggctg ccgaccccaa agcgaacaac gccggagtgg
     1261 cgaccctgca gagaacccaa gcgacgcgcg cggaagtcat tgagttcatc gtacgtttag
     1321 cccaagtgaa ataccctgag cttttctcca gcgtctcttt ggaagccgat gttacgaaag
     1381 ccctcgactt ggaaaacaaa gggatcgtcg ccgtctccga agcgcttagc aaacacttga
     1441 gaacgctgta cggacaatct tatcccgaaa acgtcggctt tgtctacgct ttcgagttct
     1501 tcttcctgtc cgagatggac actgcgaaag acggtaaggt agccgccccc ttgctgtacc
     1561 tcgatacctt ccgctctcgt atcgagtccg gcaaaaacgt tgttctcaat gagaactatg
     1621 gcgccaacag aaccgttttg gctgcgaacc cggccgaaag attctgggct gtcgcgctct
     1681 tcgcgaaagt gtttgccgcc gaaaagtcca aagtcgaaag caatatgaaa gcgcccgaaa
     1741 gaatcatcaa caacgtcatc gctttcaaac ccgtcggcgt gttcaaggga caagtgatgt
     1801 tcaataaagt cggctgcgtt gaaggagaag tatcctcgat cctttgcgct gggtgtaaag
     1861 acgccgctct cggcgccgcc gcttcggata ttcgggctac tttcctgaag ctgaaagacg
     1921 ggaagtacta cgtgctgacc gacatgaccg tttgggattc caacaacggg tacccgaacg
     1981 gcatccagtt cgccaacacc tattgcgccg atgacggtgt caatacggag aatccttgcg
     2041 aggtaggcat tgcccacccg caatctgtca cgaccgaaaa atctttcact ttggaagcca
     2101 tcggtttcgg gacgattgcg gacaacaaat ccagaatggt caatgtggac aagatctgga
     2161 tacccttgaa acagcaagaa gcctacgaaa aagtgaaaaa cgtctacggt ttggaactga
     2221 aagacatcac cgcgaaatac ccgaaaaaga tagaatctgg cgcgatagac ctctaccggc
     2281 tgttcttccc caatatcgaa gatatcacgc agaagggaag agggctagag aaagccgttg
     2341 atatgatcgt ttatggcaaa cagggcatct atgacttcga tctttacgtc aagagagctg
     2401 ccaatgccgt tgctgttgac aacctgttga agggcgagaa agtcgtattt aacatttcct
     2461 tcaacgtgaa tccgctggac ggcggagtcg ccttaaagaa agttgaattg ttacccgcga
     2521 tcgaccgttt gaaccaccga accgaagttt tgacccttaa aggtgtcaaa gatttcgttg
     2581 atctgtatgc cggcctcatc gacaccattt ccaccgatcc caaccaaccg atggtcgcct
     2641 actacaagaa actcgttgaa tttgccaaag agcacaacat tcccgatagt gcgcaactcg
     2701 tagcgatcat caagacgccg accgtcgacc gcgaagtcca atccaccacc acgcaattca
     2761 agatgatgga actcgtgctg ggtcagctgg ttcagctcga gctaaagcct gggacggatg
     2821 ccaaactctg tgatctcgaa aatgcgaacg ctttcgaccc gggttcgtca gccgccgtct
     2881 ccacttgctt cggagaagct caggatgagt atgcgatcaa caagttgaac caaacgattt
     2941 gtgaaaaaga ggcagaagcc aagaaagtct tccgcttcgg cgatatcgta gaagactgct
     3001 gcgtgacctt ggcgaaaggc tactgggctg ttaaggtcga tccttacctg gccactttcc
     3061 ttttccacga gaacacgaaa gtcgccctct tcaatgacct gggcttggtc agcgtaggaa
     3121 acgtcgattt actgaggctt gacttccttg gatttaaagg cccgatcgtc agagaagccg
     3181 ttctcgttaa accgctcgat ccgctcaacc tgttcccggc tcgtatggcc gagtcaaaag
     3241 aagagattta cgtaaaagct tacgaggtga agtaagacta aatctaattc acggtctgta
     3301 gttcaagcaa cgactacaga ccgttttttt acttagtgta tcgtctggca agggggagga
     3361 actgtgtcta agaaaaagaa atggttgatc gtttctttgt ctattgtcgt tgtctgtatc
     3421 ctggtgttgc tgctctttcc ggttaagggg attttccgcg ttactgtcga aacggcgcct
     3481 tctcagagac ccaatttgga aaccttcctg aaatcgctct tcatcagtat caaggacctc
     3541 aaagggcagg cgatacatag agaagtgttg ggggtagagc ggtatgattc tattcctcta
     3601 tcgaaaggta cctatcgaat agagatagag accttggatg gggttaatct tctctcggaa
     3661 gagctcacga tcccttgggg aaatctatgc ttcaaaccgc ggacccaaga agtgcggttc
     3721 acggtgcccc ccgcggttga aatccgccct gaaaacgttt cgttcgcctt gaaagaagaa
     3781 aagctttgga tacgctggaa aaattccggc tggtctaaaa aggctacatt ccaagtatcc
     3841 gtaaccgact tcggaacttt gacgacggaa aacaatgagc tatactttcc tatagaaagc
     3901 aacgcgttga cccaactgga cgcgttgaac gtacgcatcc gcgttttgag tgggaaccaa
     3961 taccaaacgc tctacagcgg ggaccttccc gtcaaacatt tcaagaaaac tgagacaaaa
     4021 gcgctctcgg cggaacaact cgaggtttct tgggagcacg aaaagggaac cttcaccccg
     4081 accggttacc gaatcctcgt ggaaggtagg gaatacgcga gcgtcggtcc ttccgctact
     4141 tctgagacgc tcgacctaga gacgttgaac ccccaaccga acgagggaaa gagaagagaa
     4201 ataaacctca cactagaggc catatcggat ggtatcgtcc tctctagatc cgatagcgtg
     4261 ttctatatgt atgagacggt cttctcgtcg acgctcaacg atgaatggcg tacggtgaat
     4321 gaaacggcgc ctttgaaggt cttcatcaat gaacgcccgg tggctctgcg attagagggg
     4381 gagacgataa aggccttcta tttgagcttt ctacccgagg agaccctcac agttgaatac
     4441 accgtgaacg attattctat cctccgtcgg acgatgcctt tgcgcgagga gagagaaacg
     4501 gaagccatta ccttaccatc cctttctagg gtcttttccg cgcaagcaga ggtgacggaa
     4561 gacgaaaggc tgtatctaca atggagcgca gaccgaagcg aagggaagat caaccccacc
     4621 cgttattacg tggaaacccc gcaagggacc cagacgacca ctgagaacgc gctccacatt
     4681 ccctatgaat cgaagaagca agagatcaag ctgttctccg ttttctttgg caccgtctct
     4741 tttttggaaa aagtgatcat caaaccggag aaacctggct taaccctgat tcggatcgat
     4801 tacgaccaca aggaagacgt gatcgtcgtg gagggacggg cctctataac gacgccgttc
     4861 accgttctct atcgcataga ccgttacgcc cccgaaggcg gcggctggcc cgaagagcgc
     4921 gcggggctca tctatgaggt gagtcaaaaa gagtggatcg tagcagaaga tgtgataact
     4981 tccgaggcgg gagagttttc tttttggatc cctgttaaag atcttcaggc gctacctcct
     5041 ttagaggaac ccttgaaaat caacgggtcg atcaaggttt tggacacctt caatcaatcg
     5101 gcggtgaagg acttcgaggt cgtgcgcttt ctacctccga aggtggaatt cgtaaggttt
     5161 ctcattaacg atgagaagct gttgctggag tggaaagcgg aggacccagt gttgtcgcag
     5221 aggtaccgcg atttcgcggt gaatgtgaca acgtctaccg gtgagagtta tcggtacgag
     5281 gccacgcaaa cgagtctaga aattgatttg agcgcactaa aagactatcg gttgaccttg
     5341 acgctcgaag cgttatggga aaatgagata gtccgcttgg acgaccgctc cttcgaattc
     5401 ctacccctgg atttcaatcg gttggtggat tggaatttag gaaagtcggg gaaagagacg
     5461 tggaagatcg tatattatgt caaataccag aaaggtttcg aatctatcag ctttgannnn
     5521 nnnnnncgat cacgggctcg ttcgagtggc cgattaaatc gggcaagact tatctcatac
     5581 gagcgggtat caaatacgat atcgagacga ataaagacct gatcgcccaa acggttacca
     5641 cagaagttta cgaaaccatt cctccgtcca ccggagggat aaaagaagcc tatcaaatcg
     5701 cgaaggataa gatccggctg gaagcgatag actccgcccc gggtatggaa tactattaga
     5761 ctatttcgaa caagaacaac gagcggtata ccttcaccgc cttctccccc gtttatgacg
     5821 ccgtcatctc ccggaaagac ggattaaccg gcgtgtggag cgtacacctt cgggcggttc
     5881 atagcaagac gaacaccgtt tttagcgaga cccaagacat ctatacgatg aaggccgacg
     5941 attacctcta tgaagatgtt ccggcgaaat aagagaagac gataaagacc aacaacggat
     6001 tcgtctcgcg atcgatcaag gtcaaccaag acgccgtttt gcggatcacc cagtctcgtt
     6061 tgaaatttcc gccaaaaacg agcctacgca tcgaaatggg gacgttgtac ttagaagagg
     6121 acgtacggct gaacgttatg gagaatgaga acaacgagct ttgggagggc gtttacattt
     6181 tcggtggcag agtttacgtc aaacgcgcga tagaggtgag taacgcggcg atcgcgttcc
     6241 agctttacag ttccgagggg tccatcgagg acacctcctt cttgaattgc caaaaaggca
     6301 ttctggcgca agggagtaaa ctaaaagtat ccggcaacag ccggttcacc acgattggac
     6361 tggccatttc cgcgagaaac tccgaagtga ccgtcgaaaa cactttcttt cgtaatgttc
     6421 aagaagggtt tctattcctg aataataaac tgattcggat atatcaggtg aacataacgg
     6481 gggtagagaa gcgcggggcg tatatcgcca cctccaaaga cgtcgagctt tcgaatgtga
     6541 cgattgacgg gatcgctcgt gaccaacagg aagggttttt tatcgaggat tccgtggtga
     6601 ggatgcgcaa tattaccctg aaaaacctga agacggggtt gattttcgtc aacaccgacg
     6661 cttctttgga ctctatcatc gtctatcagg tcaccgatgg gatacacctg gagtacgcct
     6721 atttcgagtt gaacaacagc acggtggaag ccgacaatct cggggttttc gcgatcacct
     6781 cgcccatcct acgcacgcga gagttttccg gattcctgcc tacgacaggg aaagttggtt
     6841 catttgccgg attgatcaga caaacgacag taaaggcccc ggtggctgta aaaatcgaaa
     6901 gaggagagaa tcatatagcg gtagagaata gccaactcat cggtgagata atccacgggg
     6961 gcattcaagt caatccaaac ggactgccct actatcgggg agagatcatc caaaagtaaa
     7021 cctgtcgatt cttggggttc actgtctctc attcaacggg ggtatttatg tataataagc
     7081 ggttttgggt agttccgctt gtgttgatat atgttttgac ctcggggtat ggcgcgttga
     7141 aagtccagaa ggtggacgtc gccttttacc cgtatgtgac cctctctgtt tctggagaac
     7201 ctttgacatc cgatcaagtt tgcctgtatg aaaacaacgt acagatcgag cctatcgtcg
     7261 atttcacgtc ttacgatatg cgatcacaaa aaccgttgga cttggtcttc gtttacgatt
     7321 tttcttggag tatgagggga tacctcgaaa gctttcagtc cgggctgtct ggttttatcg
     7381 cggaattgaa gaatctgggg tacgatacgc gcctgtcttg ggtcggtttc gccgcggaag
     7441 tgttgcacat ctacccgaac acgcagggct gtgatccacc cccggaattc acttcagacc
     7501 ttgacgcctt cgaagactat ctctcaaaag tgatgaccga agggaaaatc ccgattggat
     7561 tcgaagagtg tcagatccag gcgctttacg aagcctcgaa gtttaatttt cgagaaaacg
     7621 ccgtgaagct catcgtactt tttacggatg aggacacaca agatgacctg gagggtaatc
     7681 tttactatct ccctgatttg aaagaacaaa tagacgaaaa agggttggtc atagttcccg
     7741 tcttctcgca agaagagccg gacccgattt acgacgaatt ggctcttttt tcgggaggga
     7801 agagttatcc ttttattccg aacgaggtca tttcggacag aggatttgcc ttcggcgctt
     7861 atttttggga atttttacga agcctggccg tcgaacaaaa cacatttgcg cggataacgt
     7921 actattctcc cggaaagttc ggagagagga caggcgccct gcggcactgt acgacccaat
     7981 cggaagcaaa gttctcatac cacattcccg agacggccaa caaaatccag gtgtttggcg
     8041 tggacacccg ggacagcccg atcgtcaccc tctttttctc acccggacta ggggaggcca
     8101 ccgaagggta ccaggcggaa gaaaacggtg agaatataga tttcgctcgg tcggtgcaca
     8161 tcactcccta tcaacccgat cgagtcgatt tggtattgat tgtcgatcta gccaacagtt
     8221 cgatttccta ccccgctcaa atacaggagc acgtcgccac tttgatccag tattgcgaag
     8281 acatcggcat cagcctgcgt ttgggggccg tgatcccgct agatcaacaa atcttgtatc
     8341 aagacttgac ggaaaacctc gatcaggtcg ccaatctatt taatcggtat ttgaaagtga
     8401 aaacggtaac caacacggct gcgattccgg agatgttcca taaggcgctt aactatcatt
     8461 accgcgcgaa cagtcagaaa gccattgtct tcatcaccga cgcgttccag aacgaattac
     8521 gaaagggttt tgaatccggc gggcccctga tagacgcgat cgtcgcgacc tctttaaaga
     8581 acaacatcgg gctgttcttc atttcgcaga agaacgatta catcgactat atcaatcgga
     8641 tgacgccggg tacgtggatc ggcttcggcg agaaaccgac ccatcaatac ctgaatgggt
     8701 tttttgagga cttctttcgt cagcaacaag ctctcaccta tgaatcccga gccttctacc
     8761 agttttgtta tgacccgagt ttttttacct tgtccaaaga cggcaaacgc ttatttatcc
     8821 agttttttca attctcggac ttgagctatc aaacccgctt acaaatcaat accgtgacct
     8881 cttcgcccgc gatcctgggg aggggagaaa cgactttgct gacctgccac gcctatccgc
     8941 caaaaaacat tttctattat tgggagtgcg cggacggctc attcgtatcc ccaaacggcg
     9001 tgtcggagat cacttggaaa gcgcccgaga aaatcggccc tcataacata tacgtagaag
     9061 tctccctcgg gcagataaag atgaaaggtt cggttgtcgt caccgttcaa ccgtgagggt
     9121 aggaggacga tgtcgaaagc agccctgtta ctgatcgtat gccttgggtt caccgctttg
     9181 gggttcacgg gcggttacat caaagggatc gaactccaag gcttcccgat catacgcttc
     9241 catatgactt tagaaggtaa aataggtgat gccgcgatga cgctttacga ggacgatcgc
     9301 gaggttaaga tagagtacgt ggaagtcaag agtctgcgag aaagcccgtc ggcggatatc
     9361 gtttttttaa tagacaacag ccggagtatt agcgggtttg agaccttgat ccgcgagaaa
     9421 tcgtccgagc tgctccagct tctgatccag aataagatcg attttacgat tgcggtcatc
     9481 gggttttcgg acgtcatcaa caccgtctcc ttcccgaaaa cctcagcggc ctcgagcctg
     9541 tcgcacctca acccgttcac atcggaccca tacctcatcc ttgaaacagt ttctcgagcc
     9601 gttctgttta acgggaatca tccgagcaag cctttcgaaa tgatcgctat ggcggccgga
     9661 atgccttttc gggagaaagc gaccaagatg atcatcctct tttccgacga atgcccggtc
     9721 gaatctttaa tcaatcctga gctttatcgg gctttaggcc cttttttagc agaaaagaag
     9781 atttctttat cgatctttta tgactttcat agacaatcct tctattggga ctattataaa
     9841 cgcctagccg aatcttcggg cggacacttt ctagacctta acgagtctct attgttccta
     9901 cccgttctcg ccggcctatc ggaagaaaaa atggaactgt gggtttggtt tcggtctcaa
     9961 caaaccgcgt ttgatacgaa ggtccaccgg gtacagttca aagaccgcaa taaaacgccg
    10021 ctattcgctc cgtatgatta tcgatttccc gaggagtgga catatttgcg gttgaatacg
    10081 ataacggcta caccttttat ggtggagcgt ggggcacctg ttttcgttaa agtgtgttat
    10141 tatccttatt cagaagatac caggatcgtt ttcgataccg gtcagggctt caccctcgtt
    10201 gaacggtacg aagaggccga ggcgctgctg ttggctcctg acaaaccggg ctcttatagg
    10261 ataccggtga ccgtgtctcg agggaaagag tcacttgacg gttgtgtagt ggttcacgtg
    10321 aattagaacg gatggagggt aaggacaaat gcgaaaaaac accctacgac tattcgcttt
    10381 cgcgcttctc ctgttttgcg ggccaccgct tttttcggga gagctgttgt ttacggacat
    10441 caacaccttt cttcagtgcc cggtcgtacg tttattaaac gattacacgg tccccgttca
    10501 ggcgtaccta tccgatttcg gtgacactta ctcgcagact accacgaagc gggcgctcgg
    10561 ttggtttggc ccggtaaaaa ccgttaccca ggaaagcgcg gagatccggc gaaagttcgg
    10621 cgaggatacg gaggttacgc gcacaacgga gttggtcgtc cggttcaacg aatacgggga
    10681 aatagtagac gagaggatct acgtaccctt cggcttggat atgctccggc tcgaccgttc
    10741 gttcatcaag aagccactcg atgaaccctt ggatttgcct gatggcgtgc atttcctatt
    10801 aatacaaaga gaaacgaacc tctacgggca ggttgtggtg gagcgcatga tcgattggaa
    10861 cggcgacttg gtctatgaga tcaagaaccg gtattcggag acgaatgcca tactcgagaa
    10921 agccttctat acgtccgcag ggttcgtttc ggctcggact ttctcttatg acgacatcgg
    10981 ccaccgtttg atttcgatcc gagagcgcga cgtttggggg aggaccacct acgaaatcct
    11041 tctcgggtat gcgcgagacg accgtggcga atactacctt caatcggaag agcgcgttaa
    11101 cgacatcggt cacacggaat acacacgact gacggaccct gacggtcggt tgctagaaga
    11161 ggcaatctat gatcggtacg gtcagctcta tacgaaaaag ctgtatcaat acgaagccga
    11221 cagactggtc tttagcgaag cccacactgc cgaggggcta tacaaccgtc aaaaatggac
    11281 ctacaaagcc ggactcttgg tggagcatat ttctagcgac gccttacgca tactcgaaag
    11341 agataccttt cgatacaacg ccaccgggga gttggtggag caaaaccatt acggcgtcag
    11401 cggcgtcttc ttgtggaaga gagtctacca acctcacgcc tcccgccctt cggaaattac
    11461 ccagtacacc tctcaaggag atgtcttttc gaaacagttg tcgctctacg acgtaaaagg
    11521 gcgcctgatc tcggtcaagg tgtattcttc ctatatcgat aaagtggcgc gcgtctactt
    11581 cctcgaatcg gaagaaacct gggagtatat cgagaacgct acgaaaaagt acgtcgagaa
    11641 gaccatcaga agactagtga tcttcgatcg ttccggaaag aagctctctg atctagaggt
    11701 ggagaggcaa ttcgatgagc gcggaaacgt tttagaagag aagaccaagc tatccggaaa
    11761 ggaagtcgcc tccttcaaac gaaagttcga cgaacaaaac agggtgatag aggaagaggt
    11821 aaaaaacacc ctggagaact acaaacggca actgtccttc cacgcgtcgg gccagcccaa
    11881 agagatcatc tatctcgaag ggacgaagaa aagcaaggtc ttcgtgtacg acgaaggaaa
    11941 caacctcatc accgaagagc ggtacgacgc gaatggctcg ttgatttata aggtcgagta
    12001 tgagaggttc ttcgacgctt ggggaaattg gatcaaggtg attcgctacg attgggagaa
    12061 ggaaccgtgg gaagactcct tgaaaaagac cccgaagttc gtcgtttata ggaacttcga
    12121 atactatgaa taaatatagg gaaaggagaa gcagggtatg aagaaacttt cgatcttttg
    12181 gttcgtgatt tgtttagccg ctctctgctt tgcgctgaca ccgcaagaac tgttcgatcg
    12241 tgtcgataag gagccggtct cacgactgag ctcgtctttg gaaaagctgt ctccggaaga
    12301 cctcaagacc ctgctagcct tacgaaatgc ggaaggcgac agttttctga acgcgatcgt
    12361 gcgcctgaaa gccgaaccga tcgcgctcta tcttctgaaa aacggtgcgg acgccttcga
    12421 agtggctgcc ggtgagaccg ctttttcact ggcatgcgtg ctggatctat gggatttcgc
    12481 gatttctgcc atcgaaggaa caaaatccta cggtgaaaac caacctatcc tttgggaagg
    12541 tgttttcgaa gcggtcaaga atgacaatca ccgcctcgcg cgggtcatcc tggaaaaaac
    12601 gatcgaatac agggcgaatg catccatcgt atctcaagaa gcgctcatcg acgcaatcaa
    12661 agctcaggat gtatttttag cgttggattt gttgcgctcc ggcgccaatc ttaatgcggt
    12721 cgacagaaca ggttcgacgc ctctcattat tgcgattgcg aacggcgata accgtaatgc
    12781 ggctccgtta attttcttag gcgccgatgt ggatatccaa aatcaccgcg gcgatacggc
    12841 gggaatgttg gcggtaaaaa cggacaacct cgaagcgctc gtatggcttt tcgagaaaaa
    12901 cgccgactta tcgttaaaaa atagcgacaa caaagatatg atggacttcg cgatcgcgaa
    12961 tggcaacgaa gaaatcatct ctgccctgtt aacagaagcg attgaccaaa agcggtatta
    13021 catcctgtct gactgggtag cccgtttgga agaacagcgc gatcaaaaag cggacagcat
    13081 cgcactgaaa atggcggccg atcgaaagaa actgctcaat gaactggccg ccgcattgat
    13141 cgaatatgac aacgctttga tcaaagaccc gcaaaacgct ttggcagtct tagggcgcgc
    13201 ggacgtattc ttcctgatga aactctatga agcggctttc gaagagtatc agagggcgaa
    13261 tggactttac agcttctaca gcgaagcctg gttgggctta gccaaaacga gaatgcatct
    13321 cgcggatatg aacccggaaa ccgaacaaag aaaacctgct tatgagcaag cgcgagaaga
    13381 agcgaagcgg gctctcgaaa tcaacgtagc gtacaccgaa gcttattact atcatgggtt
    13441 gatactggtt cgcttgtccg cgttccaaga gggcgatagt cggaaaaaaa ccctggagta
    13501 cgccgtcgaa cagttcgaca aagcgttggc cctcaatgct tcgtacacgc cggcttttga
    13561 ggccaaaggc gatgctctga ggcttttggg tgagttcgag aaggccctgg tcgcttacgc
    13621 cgcctattta aagatcgacc cggaacggtt ggagataatc gaaaaacgcg gcgatactta
    13681 cgtggaaatg gctgaaaccg caaaagcgaa gtcgaaaccc aacgacgaga tcgctaacta
    13741 taaaagcgcg attactgaat ttgaaaaagt cgtccgcgtc gatgccgata acgaacgggt
    13801 ctggaagaaa gtggggaact cgtacggaaa gctccaggat atcgcaatcg atgcagcgcg
    13861 aaaggcgcag ctcaacggcg agtataaaac gcaggatgaa atggaggcca gcgccaaagg
    13921 ctatgcagag aaagcaagtc gagcgtatga gaaatatcta gaggtcctag aaaaaaagaa
    13981 gctcgaggac gacctcgaaa taagggaaaa gctgggcttt tccctttttt atcgtgggag
    14041 ctattcggag gcattgaggg tctttgctga gattctaaaa gaatacgcag acaggtatgc
    14101 ctccctgttc gggaaagcca gatgtcattt cgagttacag gagtacgaaa tggcgctgca
    14161 agtgttttca cagcttgcgg gatatccgga atacgatacc tataccgcgt tcctccacgc
    14221 ggaaacgctt ttcgcgctag aacgttacgc tgaagcgaaa gccgtttacg aaaaggctgt
    14281 tgctctcaga gatcagaaag agaagggcct gaaaattggg gaaatttggt atcaattagc
    14341 ctttatcgcc tctctcgaag ggcggtataa ggattcgata gacctggtga ataaagcgat
    14401 acaagataac ccgaccttcg cgaaagccta cttcttcaaa ggggaggaac tattcaaaca
    14461 tgaggaatac gccgaagccc ttttggcctt gatcaaggcg gtgtccttgg acggctctta
    14521 cgcagaaaaa gtccaatggg agagcaaagc ggcgcatgtt gacggggtat tcttagcctt
    14581 gaagaacgga aaagactatt ccagggctta tccgttttta aagacagcga tcggctatga
    14641 tgacgaggcg cggaaatacg atctgagaag cctgatagcg ctcacgcggt atagccggca
    14701 tagtctggac gaaaccgcct acttatggga ggttggagac tccgcgaacg ggctgaaacg
    14761 ggtgcaaacg ctcagcgacg tactgaaaac cgccgtctcg gagagaacgt tcaagagccg
    14821 aagcgaagtc atcgtgccgt atgttcaatc cgtttatatg gaaacgcaga tatcgaaaaa
    14881 aatgaccgct tctgatacca gccgctctcc gctcttaaaa acagacgccc ggattgccga
    14941 ctgtttaagg ctctgcacag acctgttgaa ggatcaaaac agaaacgccg ccggttggat
    15001 ggcgctcggt ttggtctacg aactgagaga acaggttccg ctcgccgccg tgtgtctaaa
    15061 caacgcgtac ggaatcgaac cgacgaacgc tttgtttctt ttgctgctct cggattatct
    15121 ggtaaaaagc gctcatctcg aggaaagccg cgtgttgctc tcctcgctac cggaagattt
    15181 ttcgtgggac gtcgggctgt ggttcgagcc gttcgctaga gccgaacgct ggcggctcct
    15241 cgccgagatt gcggtacgcc aaaaaaatat cccagaggcg atcgattgtt atcgctatgc
    15301 tatcgaaagc gccgagcggt tctacataga ataccggatg gaaggcgcgc ttcgcgagag
    15361 cgataccttc tacaccctcc aaaaaacgat cgccgataaa gctgaacagc tcagggtaaa
    15421 aaaagaatac tccttggctg cgactttact cgagacgggg ttaagcatac agcgattcgt
    15481 gaaccccgcg gtacgcggga agctggaact actcgcagga aagacggcgt tcacgctccg
    15541 ggatttgtca ggcgcggaaa agcttttgaa aagcgcccag gcggatctcc cgaaaaactc
    15601 cgccccatac gcagtattgg gatacgttta cctggagaga aatgaaccgc gtaaagcctt
    15661 acgggaactg gagaaagcgt tgactttggc gcaaaacgaa ggctccgata cctctcctat
    15721 cctgacggga ctggcgctcg catacttcgc tctctacctg gaagaaaaaa ccgagtcaca
    15781 gttatccagc gcgttcttct acaccaacag agcctatgcg gccgcgccgc agaacgtgag
    15841 aaacctcgtt ttcaaagggg atattttgct atccgagaac ttcaaagatc tcgccgcttc
    15901 tgtctattgt ctggcgcttc aatatcttcc ggaacactcg actatttttg agaaactcac
    15961 tcaaattaag aagtaataac gcgattcatc aggatgaaac taaaggaggg aaagagttta
    16021 tgggaaaccg ggaccaattt tatcaaaccg gcgagagccg ctcaccgttg gaagaggaaa
    16081 aaagggcgag aataatccga ggactgcgag ctttttggct attgaacacc gtcgcgatta
    16141 ttgcattgat agtggcgctt ttcgtttacg tgataccttg gaaagactca gaaatcaaat
    16201 tactcgaccc gaagatcatc attcctatcg cgctcggact aatcctcccg cttgtactag
    16261 gctttgtccc ggtagctttc acacatagcg catcatccga gaataagaaa cccgggattg
    16321 aaagcctatg gaaggagttc acgctcgtct tcatctgctg tttacttacg ctattgttct
    16381 actgtgttac tggcaaaact actatatggc ttgatatcgg gtttttatcc ttaagtgtca
    16441 gtttatatgt actctctggg atccattttc taatgcgctt aaaaacaaag gaaagttaaa
    16501 aatcccggat aatttcggag gtgtcaggtg aaaaaaccta tcgtagtatt cgtgttgtta
    16561 tcggtctcgt tgataatgat ggcgcttaac ctacctgcgg aagaaacgct ttcggagatt
    16621 tcaattgaag aggggtatgt ttttcagttg agggtctctt catctgatat cgcaaaggag
    16681 tatttcctga acgttctgaa aaacagtgaa aagaaagtca gaattgcttt accctatgaa
    16741 gcgacgggta taacaaccgt ggtgttgaaa ccggacgggt acaagttttc cgggaaggtt
    16801 cggttttgcg acggtggaga ggagtttccg tttgagtacc gaaccaatag ttacgggatg
    16861 tatgtctctt cgagctaccc aggtcttcag attctaccgg tatatccgct taatattacg
    16921 aaaaccgtta ttggcagtcc gacacgtttc gagtggaagc tcttgtcgaa tcaaatcgtt
    16981 cacgggtacg atttctggct aggaaccaat agggatgcgc tacatcttat cgcgagccaa
    17041 atcgcgaatc cctattatga aatgaacttg gaaaacttgc ggttttcggg cgctctttat
    17101 tggaaggttg aagggtacac ccgttctgaa gagagattca cgagcccggt cttttctttc
    17161 acctatcagc cttcggcaat gatgcaggcg atatgggacg cggaaagcga aaaatatgat
    17221 aagtttctcg aaaaagcgga agaaatttta gataggatga aggaaatcag cgactcatct
    17281 gataaaattt caaaattgga aacagaaatc gcgacattga cgagcgcgtc tctttctgtc
    17341 gccgggaaaa tagataccgt aataggagta gtatcagatc tttcgaccga attatcagcc
    17401 ctatcaaccc tggtggagga aaaagaaagc aaaacggaag acgcttcggg aacaatcctc
    17461 agcgaactaa aagcgggttt ttcgacgatg acgaccatag cgtctgatac gctaagtatc
    17521 gcgaaagaat tacgggcagt tgtcgtacca tccttagaaa cactcgatac tatcagcagc
    17581 gacgtgacct cctgtgtcgc ggaaatacaa aaagcgtcgt tgttctggac gccgcaacaa
    17641 aaaacggaaa tattagcgat tttggcagat tatgcaacgc ctccaaccat agaacaaaat
    17701 accgttgttg acctattcta tgagcttctc gattccttaa ccctgttaaa aaaggttgct
    17761 ctactcaaac ggctttcgga aactcacgga gagtcctggc tctttacggg tgtcgaggga
    17821 ttcacaaggt ttgtgcaggt tcttttcagt ggggaactat ggaatgccga gagtatcccg
    17881 tttgtattcg aaccctattc gaaagcgttg gacgatcttc tggatttcta catttctaag
    17941 atgaaaaaaa ccccgactgg gcaactcctt atcgatccac tttatctgct gccccaggcg
    18001 aaaaagctcc tcggtttcga aggcgcgaag catttcttta gccattattc aatcgaatac
    18061 tacacttatc atcaaacact caggaataat gagaccataa aaggaattat ttcgaaattt
    18121 tggaatggaa atggaagtga atataagata cttctacttt tgaattactt gatccatgaa
    18181 tatactatga aaaagaacga tccctctttg tctcaaaaaa tcaacgggga tgaattcctg
    18241 agtttatacc gatcgatgaa cccggatatt gaaaaatttt ttaataatga aagaaataag
    18301 aattacggaa cctcatactg ttatactttg ggttggtggg aactggtatg ggcggatatt
    18361 tggacgcctg atcc
//
LOCUS       DAOPXN010000026        18045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3164_length_18045_cov_4.755586, whole genome shotgun sequence.
ACCESSION   DAOPXN010000026 DAOPXN010000000
VERSION     DAOPXN010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3164_length_18045_cov_4.755586"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="PKM49_02600"
     CDS             complement(<1..2571)
                     /locus_tag="PKM49_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNY81737.1"
                     /translation="MPKRFLLIIFIALISYLSVFGSTVKLEVLSHPSGADIYIDRVWR
                     GTTPQVLYIAPGKHQIQLEKFGYGASSESFDLRLSSVAEYNLIPEPEILKNYPLVIYL
                     VNYQSDGDRTVLYKEQEKALVQALWNRFTNMGFNVTIETPKDEFDLVLDKESIYLDIE
                     KKYPKSGMFFFIDALWTFSSFQQKRVTRLETALKVYDPKSKVTLGTFKDLAESIGQMS
                     PDIVVLQIIERISQSFLDNIGNFLVQIYTKQTSTPILLAKTYDRDITVTTIKNLSPIE
                     NLEVADFSNNLLKAEVHTALDSGINLLFVVDRSGSNTKYSEVIKQLMDLILKNLPKRI
                     RWGILAFDDKVEVIQSFTEDYSRWTAAKEKITNAGMTRLYDGLFNAAKIVEREQGINV
                     IILLTDGIDADYLDAAPGSIKTKKEGIDALKAAGVVVYPVGISEKNFTNLMKELALEF
                     GTFYSDAFRHNNEKIATDILQDIVQSIAVVKTARIDNPSFYINGRRFYLSVNGRSLLN
                     QEMIDMAVYKTAYGELSIPVTSGDTAPSSPTLVGKPEEKLTTPPETVTTPASPIQETA
                     LASPVKPEITISTEPVSPPATQTTPHSTEVSEGPAKTDLKEEATSPATKTTQPTSVSD
                     GLVLPIQWPEDFNQILELRFSELYDLDPNGDFIWTERNTGYLWLRNQRTLLGIDLKDT
                     PVMIDLCYPYLAVLYVNRVEVATLGEKIYRLYAAPVATVPSCMTISKNGVLTLGYKNG
                     LVQAFDKAGKVLKEFKVENAALQQIVVDDQNRLIFLASSQKVGWTELSPNTIVHTKEL
                     QRPIIRIAPFLIDKARFFAVDAGGDVYYIRFDDFSEQKRNLNRGIVMQSHFAEDQNQL
                     F"
     gene            complement(2865..5621)
                     /locus_tag="PKM49_02605"
     CDS             complement(2865..5621)
                     /locus_tag="PKM49_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY81738.1"
                     /translation="MVEKKRFVGTHITLAIVLVVVLFPLFWVFTTSIRRDNAAFSTEL
                     ISSRLTLQHYKELLFKPKNLPVLIADMKQAAYISKQYKDWDEDRIQRALVGFIDQFAQ
                     HLLQTEEALKRTDEDVRGLELFYEEKKREALAGFFAERSQQEKDYELYGKQRLNELDP
                     IGAILLDAKILGHILKQGYVPEVLPILEKRFPELFKSYEAATSEVNGALKRAREELSA
                     LISGTTLSEETLASLSGSIDAFEALAREQGFSYTKWLRDVSIKLYNPLNARFSAVSEE
                     LSQRWEAFKEAQKTIAREIESALKALDQFLKTQYESVNEKKAKEVDRFIDAWQTHTQE
                     LATLQKNLKQNQERALIVKKTLEALDSDLSLLRAQIGIQSALQEKALGIIETYLAKPD
                     KLSTDKLLLERRNVFDRETESLLEEIRRLEANDDAFAKSGLLPVKESLTRVRQHQDRL
                     KRLTGYLAIDDAIQVFQNTFPVIKTICEEIRQIFETQSKTGATLVDIVFESKVLETAL
                     ANRNEEIEALSTKTAFLQNRILESNRDFDFYLLHSVLKMAEDWKGIGLFEQQSLRFLS
                     QYPKELRGPVKSPDKAIDNLNQLRTLIDSFHEQKRSVYATTATLNADVKAFKERSPLY
                     IGLRKTGAQIKVLELEEIEALYTQAAADFSSSLNRTGRKSSDLSKDDFFLPIKGALHH
                     IDKSIFEMMLHWIKKPEQFFMRWLLNSMIIAVITSFITVVVCSFGAYPYSRMRFPGRK
                     NGLLLLVLLQMFPSIMAMVALYSLLRFTGMLFPFLGLDTQGGIVFIYLGALPFNMWLL
                     KGYFDTLPNEIEESALIDGATRFQTFYKIVLPLSTPILAVVFILCFMSTFNEFVLARI
                     MLQSPQNYTFAVGLQSFSSGPYQMEWGLFTAAALIGAIPMVIVFLLIQKYLVGGLTSG
                     AVKG"
     gene            complement(5621..7363)
                     /locus_tag="PKM49_02610"
     CDS             complement(5621..7363)
                     /locus_tag="PKM49_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY81739.1"
                     /translation="MLFLRKTGFWILLILANALLLWSSILMFDSSMEGLALSLIFLLF
                     IVDFAFLHPKGYPYRYTLPALGLLLVLTVFPIYFTIKTAFTNYGTGHLLTRQQVIESL
                     LKEYVTDENALPYDYRVFMRLENLHPTEDFLLLLERSDVFYLALKPSVKQRDSRGNVI
                     SASTSIVPFRNDQALIGDSTYTIIRSKQDPERILSVNMAGPTDARPYSFFFSFDDAST
                     LPNQKYFISEVFAAYLEKADFLGPEEQVLRFGTRYGFRKLLESRKKYGLDTITVEQDG
                     KVTQRVVFVNNLTNRPLEESDGFFYDTDKDGSRTIVGGYSSFVGLNNVFQMFTDSRLS
                     IPFMKIFGWTLTWASLSVLFTFTVGLALALMLNNPRLKSRAFYRTLLIIPWAIPSFIS
                     VLVWRHGFFNETYGFINKFIVSGLMGLEPIRWLNNDFWAKVSCLIVNTWLGFPYMMTV
                     SLGALQSIPYTFYEAASIDGAGRGLRFRKITLPLLMTSIAPLLVGSFAFNFNNFVGIY
                     LLTNGGPEMPNSITNAGSTDILISYTYKLAFEGSRGQNFGYASAITLFIFLLVIAISS
                     VNFKLSGTFEEVNR"
     gene            complement(7433..8617)
                     /locus_tag="PKM49_02615"
     CDS             complement(7433..8617)
                     /locus_tag="PKM49_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY81740.1"
                     /translation="MKRVFVMVVLVSALFVSLFAAEMGKLTIWCSEAQVSILQKLGDE
                     FESLYGIPVNVQQVTFGDIRSKFLTAAPAGEGPDIIVGAHDWVGELAANGLLLPLDYF
                     TGQKRANYLESALKGFEYGGKLYGLPYGSEAIALIYNKELVPVPPSTMEELIAIAKEL
                     TDGELRGFVFNAGDFYMAAPFLFTAGGYVFKETAQGLDPNDIGLANEGAVLGGNLIKR
                     LYDEGIMMPGDNYQIMDSLFKDGLAGMIINGPWAILDIQSAGIDYGVAVIPSIAGKKA
                     KPFIGVQGFMISSKSPNHIFALEFLANFIDQKEVMYKLYLADPRIPTRADVLALVQDQ
                     PDVVAFAGSAANGTPMPNIPAMNSVWSAMAGALTQIINNLLTPEQALQEAVDKIVLAL
                     AQ"
     gene            complement(8697..9926)
                     /gene="hemW"
                     /locus_tag="PKM49_02620"
     CDS             complement(8697..9926)
                     /gene="hemW"
                     /locus_tag="PKM49_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNY81741.1"
                     /translation="MADHVTKNLLGGISLYFHIPFCQKKCHYCDYASFPIDPSYFPFS
                     QYFDLLSRECDTRLARLSHNQKNLRSLYIGGGTPSLVPTRYYGGLLRALSRHFPPEEV
                     AMAENTFEANPESVTIENLKALRSIGFNRISLGIQSLNPLTLEKMGRVHTVNRAFAAT
                     AFAKTVFDNVNCDFIVGYDPNPYDTLDPLLRFIRCFQPEHLSIYPLEIHPDTPLGKER
                     ADYPEVTSKASYPSRVFPLLQDLLRSEGYRHYEVSSYGRPGFFSRHNLRYWENADYLG
                     IGLSAGGHIQRTRYVNTADWERYLRTTLERGTPEASYLSENGAIEELKETLFMGLRTA
                     EGVDLGRLKSAWSPGDLEDFLRLFFGEEDYVIRNDRLRLTGESFFYNFEALERAMDIC
                     DRFFTERPIPGNRELRD"
     gene            complement(9896..10831)
                     /locus_tag="PKM49_02625"
     CDS             complement(9896..10831)
                     /locus_tag="PKM49_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81742.1"
                     /translation="MTKRRTFPLFGLFLICLALTLWPQSNLPASEPAKETVDWPVRFD
                     TFLGSYLIAQIPFSIGLPFDFGFGFTHEAPLGGLYAYGQYVREKKVLFAKQLIEQRQI
                     KMFLGISTDLVYEVKYTHYFSDTLDVRKVPILNQHTTLLAFLANYKVRFLGTASRPSF
                     LSDGFLKTGVGPYEVNMFYFSAWRIGSFDLSADLGIHILDYSPFSIAIGEIIGVGGLL
                     YVEKEKRFSPRLVFGFVPERGVPGIGVMFHQEIPIGETTLEIGTTFVFPFSGEGYWQV
                     QGEWKHPGDHALTFAASPKNLYLGGRWLITLPKIF"
     gene            complement(10839..11408)
                     /gene="ybeY"
                     /locus_tag="PKM49_02630"
     CDS             complement(10839..11408)
                     /gene="ybeY"
                     /locus_tag="PKM49_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNY81743.1"
                     /translation="MEKNSPVEQKSLHINIFEEQRKMKILHKGVKRLIRLVAQQEDFF
                     TPASCSVVFIRDTRMREINRSYRGADEPTDVLSFACLDRLEGTDHPRGGESGEQELGD
                     IFISVDTMLRNANEDRREVWLELTALIVHGFLHLLGYEHETEEEATEMFAKQDAYVKS
                     FAKIGNTLVRERTPTVNPPDRDRAGVADR"
     gene            complement(11395..12810)
                     /locus_tag="PKM49_02635"
     CDS             complement(11395..12810)
                     /locus_tag="PKM49_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNY81744.1"
                     /translation="MKPSLSAIKTLAKKKYYLLFFSFFLAFLISILFWQYDWKKTLSA
                     SFLVLLTNIFLCEGVAENNRFFKMHYQYTLTFYVILNLSAFFCLAIGAYFGFELLSTY
                     FGVTLITILFGRKLGQVSAVFLFFVFSLAFPSHWAFLFSCLLIGYITAELTRNVKRRI
                     ELSSVAFLIGAIQIGLYLLLVLFGFERFRADFLWKIALVPPASTVFVIGLLPYIEWIS
                     RIYSNIGLLELGNLNHPVLKSLITRAPGTYFHSTVLASFAEVAAEKIGCNPILARVGS
                     FFHDIGKITRPDYFVENQDGENPHDRIKPQISHLVLSDHIKSGIELAQKHRLPLLFED
                     MVAQHHGTRVQTFFYNKAKEIDPDVDEDAFRYEGPKPSFKESGILMLADSCEAALKSV
                     GKLDSEKARNVIKKIVLGIFEERELDNSGLSLSDLENIEEAFLGVYLNQNRSRIAYPQ
                     NRTWFEVPQKTRASERKNGKK"
     gene            complement(12813..13874)
                     /locus_tag="PKM49_02640"
     CDS             complement(12813..13874)
                     /locus_tag="PKM49_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNY81745.1"
                     /translation="MPIVKKSLAIPKSINPVFLFGQHDSFLRRIKTAFNVEIAYEGDQ
                     LYLRSDQPETADLAHKLFEEMIRMLESGHIPDEIEKQYILDTYQDLKEPFAKTAEGQE
                     TSPIAELLAREEYFFKKIKIRPKTEGQARYIETLKSNDITFAIGPAGTGKTYLAVAMA
                     IEALKSGVVQRIILTRPAVEAGESLGFLPGTLVEKVDPYLRPLYDSIFDMIPMEKFLF
                     FREKQIIEIAPLAYMRGRTLNHSYIILDEAQNTTHHQMKMFLTRIGFHSKAVITGDVT
                     QIDLKRGTDSGLVECERILKSIKGIGFSYLGKNDVIRHPLVKAIIAAYEREEELNRST
                     GSESNGYFSDPKETPKRGD"
     gene            complement(13865..14479)
                     /locus_tag="PKM49_02645"
     CDS             complement(13865..14479)
                     /locus_tag="PKM49_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HNY81746.1"
                     /translation="MSETNNGSQPVCPDVFKTLITHHTQLRSVFPGETTGALAPRPLP
                     FNRPSFDQMYMAIAEIVALRSTCLRGQVGAVITKDTRVVSIGYNGAPSKIGQCDQYGC
                     IVDSEKGGCIRTIHAEQNAIAYASRVGIPLENATLYVTISPCIDCAKLIIASGIKRLV
                     YLQEYRNSYALEYLRLSGLEVEHLNYGLNAIYNEYYSQGTSECR"
     gene            complement(14439..15812)
                     /locus_tag="PKM49_02650"
     CDS             complement(14439..15812)
                     /locus_tag="PKM49_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY81747.1"
                     /translation="MATFRAELVIRNASVFYAHRFTRVNLAIRSGKIIGVWEPTVAGP
                     EAEEIFDASGLYCFPGFIDPHVHFGLQLGSENCSDDCATGSTSAVFGGITSVFNFTPV
                     LTGAEDELAALYKEVSQSTERYRTNLFMHLTVAEPKISPKKLVELALRYKFRSIKVFT
                     TYSSSGRMTSDGYLMDLFKATRGTPLVVMVHAENDAMIDFREKKLSSDGPVKASHLPY
                     LRPTISEVEAALRVCLMVSEMGGRLYLAHISSGKTVTELYRHFSALLGKSIFIETCPH
                     YLYFSDAFLKGERAARFTTCPPLRPEEERQELLKHFYAGRIHTIGSDHCPFASWVKEK
                     YEDDYLHMPNGLAGVELGFVLLYNLLKSRENEISVEELARMFSSNVSTAFGMFPQKGS
                     LLPGADADITLFDPNAEWKVTLDTLHGRNDYDPYEGLKMKGKVDSTIIGGKFVVRNKQ
                     WVSTRVS"
     gene            complement(15812..16801)
                     /locus_tag="PKM49_02655"
     CDS             complement(15812..16801)
                     /locus_tag="PKM49_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNY81748.1"
                     /translation="MSIDFRNRHFITTQDFEKDEIDLMMDVSYDMKKKFALGEPTRYL
                     PDKTCFLIFFDQSTRTRNSMEAGMTQLGGHAHFLAPENMQVSHGETPRDTAIVLGRFG
                     HGIAIRHCLFKQGNAFLRDLCRHSKVPILNLQDDVYHPLQGLADLMTIQEKFSNNLKG
                     LKVVISWAYAETHLKPLSVPQTQALLFPRYGMDITLAYPEGFDLMPDIMETAKKNAER
                     TGSKITISHDMADSFRGADVIIAKNWGGFFVGDTEEAIRKEQAKHKDWLCDRKKMELA
                     NKHAIYMHALPADRNKEVTDEVIDGRWSVVFDEAENRLHTAKAVMALTMGGRP"
BASE COUNT         4332 a         4576 c         4635 g         4502 t
ORIGIN      
        1 gaacagctga ttctgatctt cggcaaagtg gctctgcata acgatcccgc ggtttaggtt
       61 ccgtttttgt tcagaaaaat cgtcgaaacg gatgtaatag acgtctccac ccgcatcaac
      121 ggcgaagaaa cgcgctttgt cgatcaggaa gggagcaata cggatgattg gccgttgcag
      181 ctctttcgtg tgtacgatcg tgttcggaga cagctccgtc catccgactt tttgtgagga
      241 cgccaggaat atcagacgat tttgatcgtc cactacaatt tgctgtagag cggcgttttc
      301 taccttaaac tcctttaaga cctttcccgc tttatcgaag gcttggacta ggccgttttt
      361 atatccgagg gtcaagactc cgttcttgct tatcgtcatg caactgggga cggtggcgac
      421 gggagcggcg tacaatcgat aaatcttttc tcctaacgtg gccacctcca ctcgattcac
      481 gtatagaact gccagatacg ggtaacagag gtcgatcatc acaggagtat ccttcagatc
      541 gatacctagc agagtcctct ggttcctcag ccaaagatag cccgtattgc gctcggtcca
      601 gatgaaatcg ccattcggat ccaaatcgta tagttcggaa aaccgaagtt ccaggatttg
      661 gttgaagtct tccggccatt ggatcgggag gactagccca tccgatacag acgtgggttg
      721 cgttgtcttc gtcgcgggag aagtcgcttc ttcttttaaa tcggtctttg cgggtccttc
      781 cgatacctcg gtggagtggg gcgtggtctg tgtcgccgga ggactaaccg gttccgtaga
      841 gatcgtgatc tcgggcttga cggggctcgc cagagcggtt tcttgaatcg gagaagccgg
      901 cgtggtcaca gtctccggag gggtcgtcag tttttcttcg ggtttaccga cgagggttgg
      961 cgagctgggc gcagtatcac cgctggtcac cggaatagat agttcgccgt aggctgtctt
     1021 gtagacggcc atatcgatca tttcctgatt gagcagactc cgcccgttga ctgagaggta
     1081 gaatctccgg ccgttgatat agaaagaggg attgtctatc cgggctgttt tcactacggc
     1141 gatactttgg acaatatcct gcaaaatatc tgtcgcgatc ttctcattgt tatgccgaaa
     1201 ggcatcgcta taaaaggttc cgaactccaa cgcgagttct ttcatcaggt tcgtaaagtt
     1261 cttttcagat atccccactg ggtaaaccac gactcccgcg gcttttaagg catcgatccc
     1321 ttcctttttt gttttgatgc taccgggagc agcgtcgagg tagtccgcgt ctattccgtc
     1381 tgtgagtagg atgataacat tgatcccctg ctcgcgttcc acgatcttcg ccgcgttgaa
     1441 caggccgtca tacaaccggg tcatacccgc gttggtaatc ttctctttgg ctgcggtcca
     1501 tcgcgagtag tcctcagtga aagattggat cacctcgacc ttgtcatcga aggcgaggat
     1561 tccccagcga atccttttag gcaggttttt caagatcagg tccatcagct gtttgatcac
     1621 ttcggaatac ttggtgttgc ttccactgcg gtcgaccacg aacagaaggt tgatccccga
     1681 atcgagggcg gtgtgcacct ccgctttcaa caagttgttg ctgaagtcgg cgacctctag
     1741 gttttcgatt ggggagaggt tcttgatagt ggtgaccgtg atatccctgt cgtaagtctt
     1801 ggccagcagg attggagtgc tggtttgttt ggtgtagatt tgtacgagga aattgccgat
     1861 attgtcgaga aagctttggg agattcgttc gatgatctgc aaaacaacga tgtcagggct
     1921 catttgtccg atgctttccg cgaggtcttt aaaggtcccg agcgttacct tggacttcgg
     1981 gtcatagacc tttaacgctg tttccaagcg agtcacgcgc ttctgttgga aagaagaaaa
     2041 agtccaaagc gcgtcgatga agaaaaacat ccccgatttg ggatactttt tttcgatatc
     2101 cagatagatg ctctctttgt ctagaaccag gtcaaactcg tctttcggcg tctcgatcgt
     2161 gacgttgaac cccatattgg tgaaacggtt ccaaagcgcc tgcaccaagg ctttctcctg
     2221 ttctttgtaa agaacggtgc gatcgccgtc gctctgatag ttcaccaaat agatcactag
     2281 gggatagttt ttcagaatct ccggttccgg tatcaaatta tattccgcga ccgaggagag
     2341 cctgagatca aaactctccg aagaagcgcc gtaaccgaat ttttccaact ggatttgatg
     2401 ttttcccggc gcgatataca gaacctgggg ggtcgtgccc cgccagactc tgtcgatgta
     2461 gatatccgct ccggacggat gagaaaggac ttccagtttc acggtactcc caaaaacgga
     2521 tagatatgaa atcaatgcga tgaaaattat cagcaaaaag cgcttaggca cgaaaatccc
     2581 ccccctaaac agtctatcta attgtatcac aaaacttttg acatcgacaa ccttccatac
     2641 tttgcgtttt aaggcttgcc aaccatacga tcggcgctta caagcttgac ttgctttttt
     2701 tcattcttaa aaagtaggcc gacccgaggg ttacgctccg gatcgactct acagaactct
     2761 tgcggaaatc gtactttttc tttttctatt tttggagcta cttgtcaact ctgcctttta
     2821 tgaagcccag tactctcaca tccggttaaa acggtcccgc ggcgtcagcc tttcactgct
     2881 ccagaagtga gaccccccac caggtatttt tgtatgagca agaaaacgat gaccatcggt
     2941 atcgccccta tgagcgcagc cgcggtaaac aacccccatt ccatctgata gggcccgctg
     3001 gaaaaagatt gcagtccgac ggcgaaggtg tagttctgtg gactttgcag cataatccga
     3061 gccaacacga attcattgaa ggtggacatg aagcagagta tgaacacgac ggctaaaatt
     3121 ggggtcgaaa ggggcaagac gatcttataa aaggtttgga agcgggtggc gccgtctatg
     3181 agggcggatt cttctatttc attcgggagt gtgtcgaagt agcctttcaa tagccacata
     3241 ttgaagggca aggcgcctag atagatgaag acgatcccgc cttgcgtgtc caaaccgagg
     3301 aaggggaata gcataccggt gaaacgcaat agcgaataga gggcgaccat cgccataatt
     3361 gatggaaaca tctgaagaag cacgagcaac agaagcccgt tctttctgcc agggaagcgc
     3421 atccgactgt agggatatgc tccgaaagag caaacgacca ccgtgatgaa agaggtgatg
     3481 accgcaatga tcatactgtt cagcaaccag cgcataaaga actgttcggg ctttttgatc
     3541 caatgcagca tcatttcgaa aatgcttttg tcgatatgat gcaaggcgcc cttgatgggc
     3601 agaaagaaat cgtctttcga gagatcgctg ctcttcctgc cggtacggtt gagagaagaa
     3661 gaaaagtccg cagccgcttg ggtatagagg gcttcgatct cttcgagctc tagtaccttg
     3721 atctgcgctc ccgtttttct aaggccaata tacaagggac tacgttcttt gaaggctttg
     3781 acgtccgcgt tcaacgtagc cgtcgtcgcg taaacgcttc tcttttgttc gtggaagcta
     3841 tcgatgagcg ttctcaactg attgaggttg tcgatcgctt tgtccgggct cttgaccggt
     3901 ccgcggagct ctttgggata ttggcttaag aatcgaaggg actgttgctc gaaaaggcca
     3961 atgcccttcc aatcctcggc cattttcaaa acggagtgca agaggtagaa atcgaagtct
     4021 ctgttcgatt ctagtatacg gttttgtagg aaggcggtct tcgtggacaa ggcctcgatc
     4081 tcttcgttcc gattagcaag tgcggtttcg agcaccttcg actcgaaaac gatgtccact
     4141 aacgtcgcgc cggttttcga ttgcgtttca aagatttggc gtatctcctc acaaatcgtt
     4201 tttatcaccg gaaaggtatt ctgaaagacc tggatcgcgt cgtcgatcgc caaatacccg
     4261 gtgagtcgtt tgagtcgatc ctgatgctgc cggactctcg tcaacgactc tttgactgga
     4321 agaagaccag atttcgcgaa ggcatcatcg ttcgcctcca aacgccggat ttcttcgagt
     4381 aagctctccg tttccctatc aaagacattt cgacgttcca gaagcagttt gtctgtcgag
     4441 agtttgtccg gtttcgccag ataggtttct atgattccta acgccttttc ctgtaaggcc
     4501 gattgaatcc ctatttgcgc ccgtagaagc gacaggtcgc tatcgagcgc ctccagcgtt
     4561 tttttcacaa tcaacgcgcg ctcttgattt tgcttcaggt ttttttggag cgtcgccaat
     4621 tcctgcgtat gggtttgcca cgcgtcgatg aagcggtcaa cctctttggc ctttttttca
     4681 ttgaccgatt cgtactgtgt tttcaagaat tgatccaacg ctttcaaagc ggattctatc
     4741 tcacgcgcga tcgtcttttg cgcttctttg aaagcctccc agcgttggct gagttcttcc
     4801 gaaaccgcgg aaaagcgcgc gttcagaggg ttgtatagtt tgatcgaaac gtcgcgcagc
     4861 cattttgtgt acgaaaagcc ttgctctcgc gcgagagctt cgaacgcgtc gatcgaaccc
     4921 gacagactcg cgagggtctc ttcagacagg gtcgtgcccg aaattaaagc ggataactcc
     4981 tcccgcgccc gcttcagtgc tccgtttacc tcgctggtgg ctgcctcgta agatttgaaa
     5041 agctctggaa accgcttttc caatatgggg agcacctccg ggacgtatcc ctgtttgagg
     5101 atgtggccga gtatcttcgc gtccagcagg atcgctccga tcgggtcgag ttcgttcaaa
     5161 cgctgctttc cgtacagttc gtaatctttt tcctgttggg aacgctcggc aaaaaagccc
     5221 gccaacgcct ctcgtttctt ttcttcatag aacagttcca gacctcggac gtcctcatca
     5281 gtgcgcttga gggcttcttc tgtttgcaga aggtgttgag cgaactggtc tatgaaaccg
     5341 acgagggcgc gttgaattcg gtcctcatcc caatctttgt actgtttaga aatataagcg
     5401 gcttgtttca tatccgctat gagaaccggc agatttttag gtttaaacag caactcttta
     5461 taatgctgca aagttaaccg actactgatc aactcggtgg aaaaggccgc gttatccctt
     5521 cgaatagagg tggtgaagac ccaaaacagc ggaaacagga cgaccacgag aacgatcgcc
     5581 aaggtgatat gcgtgcccac gaaacgcttc ttctcaacca tcagcgattc acttcctcga
     5641 aggtgcccga aagtttgaag ttcacggagc tgatcgcgat gacgagcaag aaaatgaaca
     5701 acgtgattgc cgaagcgtat ccgaagtttt gtcctctcga gccttcgaag gccaatttat
     5761 aggtatagga gatcaagata tccgtagacc ctgcgttcgt gatcgagtta ggcatctccg
     5821 gtcctccatt cgtcaagaga tagataccca cgaagttgtt gaaattgaag gcgaaagaac
     5881 cgaccagcaa aggcgcgatc gaggtcatga gaagaggtag ggtaattttc ctaaaccgca
     5941 agcctcgacc cgctccatcg atagaggccg cctcgtaaaa ggtgtacgga atcgattgca
     6001 aagctccgag ggagacggtc atcatatacg ggaagcccaa ccaggtattc acgatcaaac
     6061 aagagacctt agcccaaaaa tcattgttga gccatcgtat tggctcgagt cccattaacc
     6121 cggacacgat aaatttgttg atgaacccat aggtctcgtt aaaaaacccg tgcctccaca
     6181 ccaaaactga gatgaaggaa gggatggccc acgggatgat cagcaacgtc cggtagaaag
     6241 cccggctctt caggcgtggg ttattgagca tcagcgccag cgcaagcccg accgtgaagg
     6301 tgaataaaac gctcagcgag gcccaagtga gcgtccaacc gaatattttc atgaacggaa
     6361 tgctcaagcg ggaatcggtg aacatctgga agacgttgtt aaggccgacg aagctggaat
     6421 atcccccgac gatcgtacgg gatccatctt tgtcggtatc gtaaaaaaac ccgtcacttt
     6481 cttccaaagg gcgattcgtc aagttattca caaaaacgac cctttgggtg accttaccgt
     6541 cctgctcaac ggttatggta tccaaaccgt attttttccg ggattctaac agcttccgga
     6601 acccgtatcg cgtgccgaag cgcaagacct gctcttccgg gcccaaaaaa tcggcttttt
     6661 ctaaatacgc ggcgaaaacc tcgctgataa agtacttttg gttcgggagc gtcgacgcgt
     6721 catcgaagga aaagaaaaag gagtaggggc gagcgtcggt cgggccagcc atattaaccg
     6781 ataagattcg ttcggggtcc tgcttagaac gtattatggt gtacgtgcta tcgcctatga
     6841 gcgcctgatc gttcctaaac ggcacgatac tcgtagaagc cgaaatcacg tttcctctgg
     6901 aatcccgttg cttcaccgac ggtttcagcg cgagatagaa cacgtccgac cgttcgagga
     6961 ggagtaggaa gtcctcggta gggtggagat tttcaagacg cataaaaacg cgatagtcgt
     7021 aggggagcgc gttttcatca gtcacgtact ccttcaacaa actttctatc acctgctggc
     7081 gcgtcaagag gtgaccggta ccatagttcg tgaaggcggt cttaatcgtg aagtaaatgg
     7141 gaaagacggt taagacgagt agaagcccta aggcaggcag ggtataccga tagggatacc
     7201 cttttgggtg taaaaacgca aagtccacga tgaacaacaa aaagatcagc gagagggcta
     7261 acccttccat cgaggagtcg aacatcagga tcgaagacca gagaaggagt gcgtttgcca
     7321 gaatcaacag aatccaaaag cccgttttcc gtaagaaaag catcttcccc tccaaaaaaa
     7381 gggcggctct gtttcgaatc tcgaaaatcg ccgccctttt gatattgcag tgctattggg
     7441 ctaaggcaag aacgattttg tccaccgctt cttgaagcgc ttgttccggc gtgagaaggt
     7501 tgttgatgat ctgcgtcaaa gctcccgcca tagcgctcca cacggaattc atcgcgggaa
     7561 tgttcggcat cggcgtgccg ttcgccgcgc tcccggcaaa ggcgacgacg tcgggctggt
     7621 cctgaaccag cgccaggaca tcggctctcg tcggtatgcg ggggtcagcg aggtaaagct
     7681 tgtacataac ctctttctgg tcgataaagt tcgccaggaa ctcaagagca aagatgtggt
     7741 tcggggactt cgagctgatc ataaaccctt gcacaccgat gaacggtttc gctttctttc
     7801 ccgcgatcga cgggatgacg gccacgccgt agtctatacc ggctgattga atatccagga
     7861 tcgcccacgg tccattgatg atcattccag ccaggccgtc tttgaacagc gagtccataa
     7921 tctggtaatt gtctccgggc atcataatgc cttcgtcata aagtctcttg atgaggttcc
     7981 ctccgagaac agccccttcg ttggccaagc caatatcgtt cgggtccaag ccttgagcgg
     8041 tctctttgaa aacgtatcca ccagcggtga acaaaaacgg ggcggccatg tagaaatctc
     8101 cggcgttgaa aacgaaacct ctcaactcac cgtcggtcaa ttctttcgcg atcgcgatca
     8161 gttcttccat cgtggaaggg ggtaccggga ccaattcttt gttgtagatc agcgcgatgg
     8221 cttctgaacc gtaaggaaga ccgtaaagct ttccgccgta ttcaaagcct ttcaacgcgc
     8281 tttcgagata attggcacgt ttttggccgg tgaaataatc taagggcagc aacagcccgt
     8341 tcgcggccaa ttcgcctacc cagtcatgag cgccgacgat aatatcgggg ccttcgccag
     8401 caggagcagc cgtcaggaac ttcgagcgga tatcgccgaa ggttacttgc tgaacgttca
     8461 ccggaatccc ataaagggat tcaaattcat cacccaattt ctgaagaatc gaaacctgtg
     8521 cttcggagca ccagatcgtc aacttaccca tttccgctgc aaacaaggaa acaaaaagcg
     8581 cgctaactag gaccaccatc acaaaaactc ttttcacacg tactcctccc ttctttcgaa
     8641 taatgatgac tcttgtatca gacgatagac gattatagca cgtgggcgtt atgttttcaa
     8701 tcacgcaatt ccctatttcc agggataggc ctttcggtaa aaaaacggtc acatatatcc
     8761 atcgccctct ccaaggcttc gaaattatag aagaaggact ctcccgtcag ccttaaccga
     8821 tcgtttcgta tcacgtaatc ttcttcaccg aagaatagac ggagaaagtc ttctaaatct
     8881 cccggcgacc aagcggattt caatctgcct agatcgactc cttccgccgt tcgaagtccc
     8941 ataaacagcg tctctttgag ctcctctatc gcaccgtttt ccgaaagata ggaggcttcg
     9001 ggggtcccgc gctctaaggt ggtgcgaagg taacgctccc aatcagcggt gttcacgtat
     9061 cgcgtccttt ggatgtgccc tccggcgctc aggcctatcc ccagatagtc ggcgttttcc
     9121 caatagcgga ggttatgtct cgaaaagaac ccggggcggc catagctgga cacttcgtag
     9181 tgtctgtagc cttcggatcg cagtaaatcc tgcaacaacg gaaagaccct actcgggtag
     9241 gacgcttttg aagtcacttc tggataatcc gctcgctctt tcccaagggg tgtatccgga
     9301 tggatttcca aggggtatat agagaggtgc tcgggttgaa agcagcggat aaagcgcagg
     9361 agcgggtcga gggtatcgta ggggttcggg tcgtacccta cgatgaagtc gcaattgacg
     9421 ttgtcaaaga cggtttttgc gaaagccgtc gccgcaaacg ccctgttcac cgtatgaact
     9481 cgccccatct tttccagtgt gagaggattc agactttgga tgccgaggct gattcgattg
     9541 aaaccgatcg aacgcaaggc cttgaggttt tctatcgtaa cggactccgg gttcgcctcg
     9601 aaggtgttct ctgccatcgc tacctcttca ggaggaaagt gacggcttag agcccgtaac
     9661 agtcccccat agtatcgggt cggcaccagg ctgggcgttc cgccgccgat atagagtgag
     9721 cgtaggtttt tctgattatg cgacagcctt gccaatcgtg tgtcgcactc cctgcttaaa
     9781 agatcgaagt attgagaaaa cggaaaatac gacgggtcta tcgggaaaga ggcgtaatcg
     9841 cagtagtggc acttcttctg acagaacgga atgtggaaat agagagagat gccccctaga
     9901 agatttttgg taacgtgatc agccatcgtc ctcccaagta gaggtttttt ggagaagcag
     9961 caaaggtcaa ggcgtggtcg ccagggtgct tccattcgcc ttgaacctgc cagtagcctt
    10021 cgccagaaaa cgggaagacg aaggtggtgc cgatctcgag cgttgtctcc ccgataggaa
    10081 tttcctgatg gaacatcact cctattcccg ggacacctcg ttcgggaacg aagccgaaca
    10141 cgagacgcgg gctaaaccgt ttttctttct ctacgtagag gagccccccc accccgatga
    10201 tttctccgat cgctatcgaa aatggggaat agtccaagat atggatgcca aggtcggcgg
    10261 acaagtcgaa gctgccgatc ctccaagccg aaaagtagaa catgttgacc tcatagggac
    10321 caacccccgt cttgaggaat ccgtcggaga gaaaagaagg gcgggaagcc gttccgagaa
    10381 aacgcacctt gtagttcgcc aaaaaagcca agagggtggt gtgttggttc aatatgggta
    10441 cttttctcac gtctagggta tccgaaaagt aatgcgtata cttgacttcg tagaccaagt
    10501 ccgtggaaat accgagaaac atcttgatct gtctttgctc gatcagctgt ttcgcaaata
    10561 acaccttctt ttcgcgcacg tactgtccat aggcgtacaa tcctcctaag ggcgcctcat
    10621 gggtgaatcc gaacccgaaa tcgaagggca ggcctatcga aaacggaatt tgggcgataa
    10681 ggtaggatcc gaggaaggta tcgaaacgga ccggccagtc gaccgtctcc ttcgcgggtt
    10741 ctgacgccgg aaggttcgac tgaggccata gcgtcaacgc aaggcagata agaaagagac
    10801 cgaagagcgg aaaggtcctt ctctttgtca taggcacatt accgatcggc gacgccggct
    10861 cggtctctat cgggcgggtt tacggtgggc gttcgctctc tcactagggt atttccgatc
    10921 ttcgcaaaag atttgacgta ggcgtcttgt ttcgcgaaca tctccgtcgc ttcttcctca
    10981 gtttcgtgtt catagccgag aaggtgtaaa aacccgtgaa cgatcaaggc ggttagctcg
    11041 agccagacct ctcgacgatc ctcgttcgcg tttcgtaaca tcgtatccac cgaaatgaag
    11101 atgtctccta gctcttgttc tccgctctcg ccccccctcg ggtggtctgt tccctccaat
    11161 cgatccaagc aggcgaaaga gagcacgtcg gtcggttcat cagcccctcg gtagctacga
    11221 ttgatctccc gcatccgtgt atccctaata aagacgaccg aacaggaggc cggggtgaag
    11281 aagtcctcct gttgggcgac caatcggatg agacgcttga cccctttgtg tagaatcttc
    11341 atcttccttt gttcctcgaa gatgttgatg tgaagggact tttgttcgac aggactattt
    11401 ttttccattt ttccgctcag acgcacgggt tttttgagga acctcgaacc aggtcctgtt
    11461 ctgagggtaa gcgatacggg atcgattttg gttcaggtaa acgcccagga aggcctcctc
    11521 gatgttttcc aggtcgctca acgaaaggcc cgagttgtcg agttcccttt cctcgaagat
    11581 tcccaagacg atttttttga tcacgttgcg cgctttttcc gaatcgagtt ttcccacact
    11641 ttttaacgcc gcttcgcagg agtcagccaa catcaagatg ccggactcct tgaaagacgg
    11701 tttcggccct tcgtaccgga aggcgtcttc atccacatcc ggatcgattt ctttggcctt
    11761 gttgtagaag aaggtttgaa ctcgtgtgcc gtggtgttgc gccaccatat cttcgaatag
    11821 caggggcagg cggtgttttt gcgcgagctc gatcccgctc tttatatggt cggacaaaac
    11881 cagatggctg atttgaggct tgatgcggtc gtgcgggttt tcgccgtctt ggttttccac
    11941 gaagtaatcc ggtctcgtga tcttcccgat atcgtggaag aagctgccca ctctggcgag
    12001 gatcgggtta cagccgattt tttcggcggc cacttcggcg aagcttgcca gaacggtgct
    12061 atggaagtag gtaccgggcg ccctcgtgat gagcgatttc aggaccgggt gattgaggtt
    12121 tccgagctcg agcaacccga tgttcgaata gatacggcta atccattcga tataagggag
    12181 aagaccgatg acgaacaccg tggaggccgg agggacgagc gcgatcttcc acaggaaatc
    12241 ggctcgaaac ctctcgaagc cgaacaaaac caggagtagg tacaacccga tctgtatcgc
    12301 cccgatcagg aaagcgaccg aagagagctc gattcttctc ttcacgtttc tcgtgagttc
    12361 ggccgtaatg taacctatca ataggcagga gaacaagaag gcccaatggg aaggaaaggc
    12421 caaggaaaag acgaaaaaca aaaacaccgc ggagacttgc ccgagcttac gcccaaacag
    12481 aatcgtgatc agggtgacac cgaaataggt ggataacagt tcaaacccaa aataggcgcc
    12541 gatcgccaaa cagaagaagg cggagaggtt taggatcacg taaaacgtga gggtatactg
    12601 gtagtgcatc ttaaagaacc gattgttctc cgcgacgccc tcacataaaa agatgttcgt
    12661 taaaagaacg aggaacgagg cagagagggt ttttttccaa tcgtactgcc aaaacaggat
    12721 agagatgaga aacgccaaaa aaaaggaaaa gaacaacaga taatacttct ttttcgccag
    12781 tgttttgatg gcggaaagag agggtttcac gattaatctc cgcgcttcgg tgtttctttg
    12841 ggatcagaaa agtacccgtt cgattccgaa cccgtcgacc gattcagctc ttcttcccgc
    12901 tcgtaagcgg cgatgatcgc tttgaccagg ggatgacgga tcacgtcgtt ttttcctaaa
    12961 taggaaaatc cgatgccctt gatgcttttc aagatcctct cacactccac caatcccgag
    13021 tcagttccac gtttcaagtc tatctgggta acgtcgcccg taatgaccgc tttggaatgg
    13081 aaaccgatac gcgtcaagaa catcttcatc tggtggtgcg tcgtgttctg cgcctcatcc
    13141 aggatgatgt aagagtgatt cagcgttctt cctctcatat aggccaaagg cgcgatttcg
    13201 atgatctgct tttctcgaaa aaacaaaaat ttttccatcg ggatcatatc gaaaatggag
    13261 tcgtaaagag gccttaagta cgggtccact ttctcaacca aagttccggg caaaaacccc
    13321 aggctttctc ccgcctctac ggccggacgg gtgaggatga tgcgttgcac gaccccggat
    13381 ttcagggctt cgatggccat cgcaacggcg aggtaggtct ttcccgttcc ggccggtccg
    13441 attgcgaacg tgatatcgtt actcttcaaa gtctcgatgt accgagcctg accctccgtc
    13501 ttcgggcgga ttttgatctt tttgaaaaag tattcctctc tcgccagcag ttcggcgatt
    13561 ggggaggtct cctgcccttc ggcggtcttc gcaaacggct ctttcaaatc ctgataggtg
    13621 tccaagatat attgtttctc gatttcatct ggtatatggc cgctttccaa catccggatc
    13681 atctcttcga agagtttgtg ggccaaatcg gctgtttccg gctgatcgct tctcaggtac
    13741 agttgatctc cctcgtaggc gatctccaca ttgaaggccg tcttgatacg tctgaggaaa
    13801 gaatcgtgct ggccaaataa aaagaccggg ttgatagact tcggtatggc gagcgatttt
    13861 ttcactatcg gcattcggac gtcccttgac tatagtattc gttgtaaatg gcgttcagac
    13921 catagttcag gtgttctacc tccaaaccgg agagtcggag atactccaac gcgtagctgt
    13981 tcctatattc ctgaagatag accaatcgtt tgattccgga ggcgatgatc aacttggcgc
    14041 aatcgataca ggggctgatg gtgacgtata gcgtcgcatt ttcgagcgga atcccgactc
    14101 tggaagcgta agcgatcgcg ttctgttcgg cgtgtatcgt cctgatgcaa ccgccctttt
    14161 ccgaatcgac gatacacccg tactgatcgc attgaccaat ttttgaaggc gccccgttgt
    14221 agccgataga caccactcgg gtgtctttgg tgatcacggc gcccacctgt cctcgcaaac
    14281 acgtgctcct taaagcgacg atttcggcga tggccatata catctggtca aaggaaggcc
    14341 tattaaaggg aaggggccta ggcgcgaggg cccccgttgt ctcgcccgga aaaaccgaac
    14401 gcagttgcgt atggtgggtg atcagggtct tgaaaacgtc aggacacacg ggttgagacc
    14461 cattgtttgt ttctgacaac gaacttccct cctatgatcg tagagtccac ttttcctttc
    14521 atcttcaacc cctcgtaagg atcgtaatcg tttctcccgt gcaaggtatc gagggttacc
    14581 ttccactcgg cgtttgggtc gaacaacgtg atatcggcgt ccgcgccggg taaaaggctc
    14641 cccttttgcg ggaacatgcc gaaagcggtc gaaacattcg aagaaaacat acgagccagt
    14701 tcctcaaccg atatttcgtt ttctctcgat ttcagcaaat tgtagagcaa cacgaacccc
    14761 aattccacgc cggcgagccc gttgggcata tgaaggtaat cgtcttcata cttctcttta
    14821 acccaagaag caaatgggca atggtccgat ccgatcgtat ggattcggcc agcgtagaag
    14881 tgcttgagca gctcttgacg ttcttcttcg ggccttaaag gtggacaggt ggtgaatcgg
    14941 gccgcccgtt cccctttcag aaaggcatcg ctgaaataga ggtaatgcgg acaagtttcg
    15001 atgaagatag acttacccag caaagcggag aaatgacgat agagttcggt cacggtttta
    15061 ccgctggata tgtgggcgag gtaaaggcgc cctcccatct ccgataccat caggcaaact
    15121 cgaagggccg cttccacctc gctgatcgtc gggcgtaagt agggtaggtg ggaagcttta
    15181 acgggcccat cgctcgagag cttcttttca cgaaagtcga tcatcgcatc gttttcggcg
    15241 tgcaccataa cgaccaaagg cgttccgcga gtggctttaa acaaatccat caggtaaccg
    15301 tcagaggtca tccgaccact actgctataa gtcgtgaaca ctttgatcga ccgaaatttg
    15361 tagcgtaagg cgagctccac cagctttttc ggagaaatct tcggttctgc aaccgtcaga
    15421 tgcataaaca gattcgttcg gtacctttcc gtcgactgcg aaacctcctt gtacaaggcg
    15481 gccagttcgt cttccgcgcc agtaagaacc ggagtgaagt tgaaaaccga tgtgattccg
    15541 ccgaatacgg ctgaagtgga gccagtcgcg caatcgtcac tacaattctc cgagccgagc
    15601 tgaaggccga agtgcacgtg gggatcgatg aaacccggaa aacagtaaag acccgaggcg
    15661 tcgaaaatct cctccgcctc agggccagca acggtgggtt cccacacgcc gataatcttt
    15721 ccggatctga tcgccaggtt aacccgcgtg aaacgatgcg cataaaaaac cgatgcgttc
    15781 cgaatgacga gctcggctct gaatgtggcc attaaggccg tcctcccata gtgagcgcca
    15841 taaccgcttt cgcagtgtgc aggcggtttt cagcctcatc gaagacgacg gaccagcgcc
    15901 cgtctatcac ttcatcggtc acctctttat ttcggtctgc tggcaaggcg tgcatataaa
    15961 tcgcgtgctt attggccaat tccatttttt tcctatcgca gagccaatct ttgtgtttgg
    16021 cctgttcttt gcggatggct tcttctgtgt ctcctacgaa aaaaccgccc cagtttttcg
    16081 cgatgatgac gtccgctccc ctaaaggagt ccgccatatc gtgcgagatc gtgatcttcg
    16141 atccggtcct ttccgcgttt ttcttcgcgg tctccataat atcgggcata aggtcaaagc
    16201 cttccggata ggccaaagta atgtccatgc catacctcgg gaacagcaaa gcctgcgttt
    16261 gagggacaga cagcggcttc agatgcgttt cggcgtaagc ccaagagatg acgactttta
    16321 accctttgag gttgttggaa aatttttcct ggatcgtcat caggtcggct aatccttgta
    16381 aggggtgata aacatcgtct tggaggttca agatcgggac tttggagtgg cggcacaggt
    16441 ctcgcaaaaa ggcatttcct tgcttaaaca gacaatgtcg gatggcgatc ccgtgcccaa
    16501 accggcccaa gacaatcgcg gtgtcgcgcg gcgtttctcc gtgtgagact tgcatatttt
    16561 ccggcgcgag gaagtgggcg tgtcctccca actgggtcat gccggcctcc atagagttcc
    16621 tcgtccgcgt agattgatcg aaaaaaatca agaagcaggt cttgtcgggt aggtacctgg
    16681 ttggctcccc taaggcgaac ttctttttca tgtcgtaaga aacgtccatc atcagatcga
    16741 tttcatcttt ttcgaaatct tgtgtggtaa taaaatggcg gttccgaaaa tctatactca
    16801 cacgaaatcc tccttcataa gatttgccga agcggcaaga ttatatcata ttttcgcggg
    16861 cgtccgtgga taggtgttgg tcatgttatg gggtgacatc aagaaacgag gttagaatcg
    16921 atcgttcgat ttttaatccg tgttgtcatc gcggcgtaga aggtgctaag ggcggatcac
    16981 ggggtcataa aaaccactta caccgcgata acacacgcga gcatcgcggc gtaagcgctt
    17041 cttctttttg cgacggcgcg atacgctttt gtacacgccc attgcggcgt cttaaaaaac
    17101 aaaatgcgta tcgcggggtc ccgttggtta ccccgcgatg aacctcttag ttatgtcacc
    17161 tcttaaattg tctcacacgt ggataggtgt gggtcaagtt atgaagtgcc ccaagaagca
    17221 aggccagaat cgatcgcgcg atttttagtt cgtgtcgtca tcacgtcgaa aacgctaagt
    17281 gcaaagacgg atctcgagat ttcgcgggct gggaacgccc tggtggcgcc gacttccctg
    17341 tcggcacgtg tctgggagcg ccctgggaac gccggacttt agtccggcat gtctttcctt
    17401 tattccggga gaacgctatc ccttgccgat tttccctctg tgttctctgc gtcttccgct
    17461 tgaaccgtgt tttcaagaca agtacaaaag cgtatcacgc cgtcgcgaaa agaaggggcg
    17521 cttacgccgc gatgcagcgc ttgggaacgc tcccagtgaa aagataaaac gatgccaaac
    17581 taaagtttgg cgttcccagc gcgttcacaa aaatcttttc tctgtgttct ccgtgccctc
    17641 tgtgcgaacc ctgtttttca agacttgtac aaaagcgtat cgcgccgtcg cgagaagaag
    17701 tggcgcttac gccgcgatgc agcgcttgcg aacgctccca gtgaaaagac aaaacgatgc
    17761 caaactaaag tttggcgttc ccagcgcgtt cacaaaaatc ttttctctgt gttctccgtg
    17821 ccctctgtgc gaaccctgtt tttcaagact tgtacaaaag cgtatcgcgc cgtcgcgaga
    17881 agaaggggcg cttacgccgc gatgcttgtc ctctctgtgt tctctgtgcg caaaccctgt
    17941 ttttctttta agacatgcca acttgaaagt tggcgttccc aggcgttctc ggcccgactt
    18001 tcgaccggtt ttggagaaaa agggaaaaaa taagaaaaaa caacg
//
LOCUS       DAOPXN010000027        17867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3209_length_17867_cov_5.240905, whole genome shotgun sequence.
ACCESSION   DAOPXN010000027 DAOPXN010000000
VERSION     DAOPXN010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17867
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3209_length_17867_cov_5.240905"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..983
                     /locus_tag="PKM49_02660"
     CDS             150..983
                     /locus_tag="PKM49_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNY81749.1"
                     /translation="MLSAVFWLLVVTAIWGTTFPLQKMVLGGVSPFVYNAIRFWIATI
                     FAFIIQPKQNLKYGLLIGLLLTCGYISQTWGLTLTTASKSGFITSLYIIFVPVVSYFW
                     EKERLTFFQKIGFPLALVGSYFLSGGISGFNFGDFLTLLCALSFSVHVVSLTVFARKT
                     SETHLMGWQFGTVAITNTLLGLNQSWSVPLPAWGVALYTACFATVIGIFLQMKYQKKI
                     GSNTAALIFVGEPIFSTLFAMLFLQESLTSFQWFGALLLLVALAFASVKAKEPNSKER
                     G"
     gene            1104..1179
                     /locus_tag="PKM49_02665"
     tRNA            1104..1179
                     /locus_tag="PKM49_02665"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1137..1139,aa:Thr,seq:cgt)
     gene            1278..2687
                     /gene="tilS"
                     /locus_tag="PKM49_02670"
     CDS             1278..2687
                     /gene="tilS"
                     /locus_tag="PKM49_02670"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNY81750.1"
                     /translation="MIAVSGGVDSMVLLHVMLAGLPERRSCFCVAHLNHGIREASLSE
                     YAGVRQHCDREGVCFYGETIDVPGLLKTAYPGRSLEDAARIERRNFLTRAASSWGSKV
                     IVTAHHRDDLLETFLMRLLRGSGLSGYDCLKAVDPPFHRPLLTFWKADLLRYARAHRL
                     LYYEDHTNLDNRFLRNRIRNELVPFLSSRFGPRIPDVLIRDQELLSSASQLIDELLEP
                     YIHSAVYTKDRVTFSWEDLKEYSDAFLSELFVRACKRWRGHPYGIEAGKLTRAIERSR
                     LAGHFVLPFRQDLWFFREYDLCGFTSQKPERVCALERAGSGSDEGVWENPDRPFEFRT
                     QDGRKVTFTAERGIRPERIDSLKTDRWTALFDEDKVQFPLTLRAPISGDRFRPLGMRE
                     EKRLARFCSDQKIPIRDRQELLLLINRKSDIIWCIGIRIADTVKITLDTRHILRVRCQ
                     LEPEKGIEGRERSIPQFFD"
     gene            2803..4707
                     /gene="ftsH"
                     /locus_tag="PKM49_02675"
     CDS             2803..4707
                     /gene="ftsH"
                     /locus_tag="PKM49_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNY81751.1"
                     /translation="MPNKKPPRPTNQLFFLLLMIFLVVLIFFAYKSPNFSSVQAVDFS
                     TFWSMVKDGKVREVVIEDTGNIQFKTTTGFQETLYETYVPWVMNNPDILGELIEQGVK
                     IKSQKSSGDFIWALLLNVLPLLLFVFIFFGMMRSMSGKNNQAFTFTKSPARMVSASKK
                     RVTFKDVAGVDEAVEDLQELVIFLKSPQKFDKMKARMPKGVLLVGPPGTGKTLLARAV
                     AGEANVPFFHMSGSDFVELFVGVGAARVRDLFNQAKNAAPSVIFIDEIDAVGRHRGAG
                     LGGGHDEREQTLNQILVEMDGFEINEGVIVLAATNRPDILDPALLRPGRFDRKVVVDP
                     PDVRGRESILNIYLKKRPLDPEIEVSVLARRTPGFVGADLENLCNEAALLAVRASKDL
                     IGMSELEEAIDRVIAGPERKSRIISPKEKRIIAYHELGHALVSSALKNADPVHRVSII
                     PRGHSSLGHTLQLPLEDKFLMSKSEIYDKISSILGGRAAEEIVFDEITSGASHDIERA
                     TEMARWMVCQLGMSSALGPISWGKEEQEVFLGRDFSRMKNFSDDTAKVIDDEVKKIIE
                     KCYNRAKEIIRKYKNLLDRLAEILLEKEVIDGEFLREMLCTEMAKENHKQLEAQIEAQ
                     IEPTLIKELG"
     gene            4726..5175
                     /locus_tag="PKM49_02680"
     CDS             4726..5175
                     /locus_tag="PKM49_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81752.1"
                     /translation="MKKNLWLLLFAFVVTIGCGMALWAGGGVFYLEEYPHPALNFAIT
                     DRFFSIEHSGTYIDHFGFANFLDLYGKIDWVITPIVGLGTCWGISYDDLLFIERGGFY
                     VGGGVLLSYGRYSLQLVLKHYIPFFQEIVFSPSVQLIGRFKIGEWWE"
     gene            5172..6893
                     /locus_tag="PKM49_02685"
     CDS             5172..6893
                     /locus_tag="PKM49_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81753.1"
                     /translation="MKKSVLFLIMTIFVFVFFTGCIKPNPASSITVTIDSPTSITVGS
                     AANFTVKLSSSKLGGIGNLFAKDPVISKVTWNFTHQTDSSKNFQFTATSATTNYTFGS
                     PNVFFTGTFVLSVSVEDKDGNSYQSKSTNNKTVEIKASTPSFLILPKDTGDHEISLSE
                     LKKNQIVKFVLSDLTSEGISNSQIQNYQIRWALSKWDEASSSYKEIPALVQEYRIYAT
                     SNSYQVPIPHTGKLALKISIKDIFGNVTSFERAKTFDVVFAIPEAPQLLEHDWTGDGK
                     KYRLVFSKSADAYYYEIYRQAVSLSTERIQTSQIEEFIGRAFLTDAEKVVFDDPKPTK
                     GWVVYSVYAIDGGGASAKTEFSINVANRKPGKAKLISPIGLVTSVPDPVAGLWAYWSE
                     GEDVDPGDTVTYLVYVKEGSNFVLTNRTTSKSAAINYLFANGIVYSIRVDATDGISTT
                     TGDVSTFWIIDGSVQPPRIEEVIPLYLKDPAYLSTRVTFSHINPYGAMTGILTRYVIQ
                     FSRYSTFPPADTFEGEVIAPGTHTLNIPKRYVKENEPLYARVRAIVSTNHILFQSNWS
                     NVVRFYK"
     gene            6933..7760
                     /locus_tag="PKM49_02690"
     CDS             6933..7760
                     /locus_tag="PKM49_02690"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNY81754.1"
                     /translation="MNYDQYQKQVKEAAEYIKRFVKERPKLGIVLGSGLGSVAAALTD
                     PFTLPYKEIPHFPVSTAPGHKGELVFGRIGALQTLLMSGRLHAYEGFSMREIVFPIRV
                     MQLLGTEMLFVTNASGGLNPDFELGVPMLITDHINFMGNNPLVGQNYDTWGPRFPDMS
                     EVYDLELRKKAVEAARELGIKLQEGVYVAVTGPNFETPAELRMLRRLGADAVGMSTVP
                     EVICAVHAGIRVLGVSAITDRAVAEDLEPLTAEQVLEIAEKTGHKIASIFLKITERL"
     gene            7773..8537
                     /locus_tag="PKM49_02695"
     CDS             7773..8537
                     /locus_tag="PKM49_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNY81755.1"
                     /translation="MLEFIVLSLVQGLTECLPVSSSGHLFLLSRWIGYDLSLATAVGL
                     HLATLLAVILYLRKECRQILAGMLQFDKAYWRLFWGFLLASVPAALVGFLVKDSLETA
                     FANPTAIGINLLITGGFLFLSDFIKRDQTPSMSLTQITWRQAFAIGLFQSVAVLPGIS
                     RSGLTLVGCLVVGLPRELAFRYTFLLSIPVILGSGLLELRSFSVSSELVPGYVIAFFI
                     ALLALFLFRKPVLRSRLRYFAYYCWILGALLLLILR"
     gene            complement(8687..8929)
                     /locus_tag="PKM49_02700"
     CDS             complement(8687..8929)
                     /locus_tag="PKM49_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81756.1"
                     /translation="MGVERLTGNMPQGELISKTENKRNRVVENSETNGKPALVETETL
                     EKSDFGSLRAEVLRLDPAALDSLHHMTRERVIALLA"
     gene            9118..11469
                     /locus_tag="PKM49_02705"
     CDS             9118..11469
                     /locus_tag="PKM49_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNY81757.1"
                     /translation="MVDFRTDRKTGILLLFFLCFVYASLFGVAEIKIGIYQNSPKVFL
                     NERGEPAGFFVDIVNEIVKSVGLRVSYVFGTWAENIAKLDWGELDMLVDVSYTDERAA
                     RFSLNSIPIIESWIQVFSLPETTIFRAQDLGGKRIGALSDSTQYRYLRDKLKEDFSID
                     YTIETFSDYPHQVEALIGRKIDLFLADRFFYFSKDRPQNVIPKPVILQPNGVYFAFRK
                     GYDPGIVGAFDTALFSMKNDYGSPYYRALNQWFNLSIETTFPFWLKTLLVSILVLMTM
                     LSVFLFLRDYRRTKAHNRELERRVSERTEHLNLALHQAQSANEAKSVFLSSMSHEIRT
                     PLNAIIGFAQILRRDATLTRKQQEQLQTIAQSGEHLLKLINNILDFSKIEAGKLSLDL
                     SEFDLNTLMESLVNIFRLRAEEKGLRFFYEPKLPVPCYVRGDEGKIRQVFINLLGNAD
                     KFTQTGGIVLRARITLRQDSGERGSFADFVAEVEDTGPGIPEEEVPRLFESFHSLKAG
                     RAYGGTGLGLSISKRFIDLMGGSITYEHRPRGGSIFRLRVCLDKVNALAAAPRSEDRT
                     IIGLKPGTVPPRVLIVDDQAENRELLRDILDPIGFQTRLAENGESAIEIFSRWSPDIV
                     LMDMLMPVMDGYEATKRLKSTEEGRQVPIIAVTASAFGDEEKKVLETGVDGYLRKPLR
                     GEDLLALLSEQLGITYLYAPTANQDTLSDPPGQPRPLTAEDFDFLSDDEIAEMRKALS
                     NGEMLRLRELIAQIGEKRPFLAERLLVLAKQYNYEDLSRLLER"
     gene            11483..12640
                     /locus_tag="PKM49_02710"
     CDS             11483..12640
                     /locus_tag="PKM49_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY81758.1"
                     /translation="MNPDLEGASIMIVDDTPANLELLAEMLHAKGYRVLQFPNGMTAL
                     HACVRVKPDLILLDIMMPQMDGFEVNRRLKTDEKLRDIPVIFISALDDTANIVQALSQ
                     GGVDYITKPFKEEEVLARVRTHLKIVFLQRQLKAHNENLEELVAKRTQQLTRAHERLK
                     ELDRLKDDFLSMISHEIRTPANGLLGVGELLIELCPPSENARNYEEMFRECSSRLRKL
                     IDDASLLGDIDRLIEDTDIDVSFSDLLQEIRESFPEVRITVETQVDPEKVFFKADPVL
                     LKKAFETTLLLARKFSSKKKPVSLLGLAGEQHLSVQIPIDLLYLTHEQAETFFNIEST
                     FRASSQAESMGLAPVVAHKILSAFGGGLNLVKQEGTKGYVEAIMLTQERIT"
     gene            complement(12718..13731)
                     /locus_tag="PKM49_02715"
     CDS             complement(12718..13731)
                     /locus_tag="PKM49_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81759.1"
                     /translation="MAASAILRVEKLKKYFPVKSLPFSRKRSFLKAVDDVTFTLHAGE
                     TLGIVGESGCGKTTVGRTIMRIYEPTSGQCWWRVGDSAETMEDFFCYKNAQMRRIRHQ
                     IQMIFQDPYASLNPRMTVQDIISEGLKANGLVSGGREMINTVADIMEKVGLLPEYMKR
                     YPHEFSGGQRQRIGIARVIVMRPKVVICDEPVSALDVSVQAQIINLLEDLRSEFGLTY
                     LFIAHDLSVVKHISDRIAVMYLGKIVEKTDTDRLFEAPLHPYTQGLLQAIPVPDPHQR
                     KSGHRMLIAGDIPSPIDPPEGCRFVKRCSKAMDRCRVETPALLEVREGHEVACFLYPN
                     PRE"
     gene            complement(13703..14725)
                     /locus_tag="PKM49_02720"
     CDS             complement(13703..14725)
                     /locus_tag="PKM49_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81760.1"
                     /translation="MEDRILKVENLEISFKTQFGRVKPVDEVNFYLDRGETLGIVGES
                     GCGKTVTAFSIIRLLPKNAILGERSRICFEGKDLAECSKEELQKIRGKKISMIFQEPM
                     TSLNPLFTIGQQLAEVYRLHEGLSEKEAKQRSIGLLEMVRIPEPKKRIDDYPHLLSGG
                     MRQRAMIAMALACKPSLLIADEPTTALDVTIQAQILELIHELKTAQNTSTIVITHDLG
                     VVAEMCGRVIVMYAGQILETGGIFQIFDHPSHPYTRGLINSIPKLTIDKKSQGKLHVI
                     EGYVPHPSSFPKGCRFNPRCEKAFPKCVQEPPPLFEIESGHRVKCWLYAEEGEVPGGC
                     VSDTPS"
     gene            complement(14741..15679)
                     /locus_tag="PKM49_02725"
     CDS             complement(14741..15679)
                     /locus_tag="PKM49_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81761.1"
                     /translation="MGKSFRWYRKKVEKIEEAASPLNIVTYRKLVWIKFKTHRLALMG
                     AVILILLVLFSFVGPLFTSVSYEDMDFGSIFSPPFTEGHFLGTDELGHDVLVRLMYGG
                     RVSLFVGFVSALLTTIIGTAIGLFSGYYGSILDRLLMRFVDIMLSIPTLPILLTLTLV
                     FGSGISGIILVLTVFGWMGVARLVRGLTLTIRESEYVVSAKAVGVRNLSVLFKHVLPN
                     VLPVIIVSATLNVSYSILTESSLSYLGLGIQPPMPSWGNMLQKSMNYILGTAMGVTPW
                     WLTFFPGMMIFFAVLNVNFLGDGLRDALDPKFTSHT"
     gene            complement(15688..16671)
                     /locus_tag="PKM49_02730"
     CDS             complement(15688..16671)
                     /locus_tag="PKM49_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81762.1"
                     /translation="MKVYFLKRFLELIPVFFAISFIIFMLINLMPGDPLTQMRMENPR
                     SFMNDPERLKALREYYHLDDPLAIRYLTWLKSMLTGDFGYSSMYKQPVKDLIFSRLPN
                     TLYLTVVSWLLGLVVAMPIGILSALKKYSVFDYTATVAAFIGISLPSFWFALMAIIVF
                     SVQLKWLPVSGMSTYGLTGQWRVFWDSVRHLILPACVLGFVQVASWVRYIRTSMLEVL
                     DQDYVRTAYAKGAPERKVILRHALKNAMIPIVTIIALDIPYFFGGALIIETIFSWPGM
                     GRLMYQAVIGSDYNLAISCLMFLTIITLISNLVADLLYVLIDPRIRITRKA"
     gene            complement(16676..>17867)
                     /locus_tag="PKM49_02735"
     CDS             complement(16676..>17867)
                     /locus_tag="PKM49_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HNY81763.1"
                     /translation="ALLFGCPHPNEPIGAMMLDALSELLAKDDPRLRELDYTWYLIKS
                     IDIDGTKLNEKWFADSSSIRKYAENFFRPAGYQQVEWTFPIEYKTLKFNKPLPETQVL
                     MDLIKEKKPIFLYSLHNSGFGGVYYYVTKDLGEGLFKKYQELPGLFNLPLNLGEPEAP
                     FLKKLAPAIFQLFGMKEEYDYLEKHLPPGTDPATIIRAGTSSDDYAGAVADSFTLVCE
                     MPYYYDARVNDQTHLPETRQHYLRESLGRSKERYAALKQIFDAIDRVIQDRDSPFYHA
                     LTNYLETVPGSLKATENWIETDETLKNPATVAEMFDNTYSTRFYESLSLGMLRRLLDE
                     NIVLGGDRAVLEKAKKTADEMFETTIAFLEKHLRYHAIPIKDLVSVQMLAGLNTMAVL
                     QQKE"
BASE COUNT         4316 a         4541 c         4647 g         4363 t
ORIGIN      
        1 ccttcgtctc gaaacgtgtt ataatcccga ggttttgccc tacgcgaaat atacgggtgt
       61 cataaaagat ccaagagaac gcttcgagcg cgcggtcaga agcgaacggt gagggtgcga
      121 aaagcgaagc gacgcaagga ggagtcggta ttttaagcgc tgtcttttgg ttactcgtgg
      181 ttacggccat ttggggaacg accttccctt tgcaaaagat ggtcctagga ggggtttctc
      241 ccttcgttta caacgcgata cgtttctgga tcgcgacgat cttcgctttt atcatccaac
      301 cgaagcaaaa ccttaaatat gggctgctga tcggcctcct gttaacctgc ggctatatct
      361 cgcagacctg ggggctcacc ctgacgacgg cttccaagag cggcttcata acctcccttt
      421 acattatctt cgtgccggtc gtgtcgtatt tttgggaaaa ggaacggttg acgttctttc
      481 agaagatcgg ctttccactc gccttagtcg gttcctattt cttgtcgggc ggtatctcag
      541 ggttcaactt cggcgatttt ttgacgttgt tgtgcgctct tagtttttcg gttcacgtcg
      601 tcagcctgac ggttttcgcc cggaagacct ccgaaacgca cctgatgggt tggcagttcg
      661 gaacagtggc gatcacgaac accctcctcg gattgaacca gtcgtggagc gtaccgctgc
      721 ccgcttgggg ggtggcgctc tataccgcct gtttcgccac ggtcatcgga attttcctac
      781 agatgaagta tcaaaaaaag atcgggagca acacggccgc cttaatcttc gtcggggaac
      841 cgatcttttc caccctgttc gcgatgctgt tcctgcaaga aagcctcact agctttcaat
      901 ggtttggcgc acttttgctt ctggtcgctc tggccttcgc gtcagtcaaa gcgaaagagc
      961 caaattcgaa agaacgcggg tagtcgcccc gctttttttc tattgaattg gcaagcgcta
     1021 tcgttttcaa cctattcgca cgtctgggtg aaaaaaaccg acttgcaaaa gccggttcga
     1081 tctggtagaa tatgccaacg gaagccagcg tagctcagtc ggtagagcgg ctcactcgta
     1141 atgagcaggt cacgggttcg attcctgtcg ctggctccat atttttggag ggctattcga
     1201 tcgttaacga tcttatttcc gatatctccc gtgtggtcgc tttctttttc tctcgttttc
     1261 ctatagagac tcggtttgtg atagcagttt ctggcggcgt tgattcgatg gttttgcttc
     1321 acgttatgct tgcggggctg cccgagcggc ggagctgttt ttgtgtcgcg catttgaacc
     1381 acggcattag agaggcctcc ttgtccgagt acgccggagt gcggcaacat tgtgacagag
     1441 agggcgtctg tttttatgga gagaccatag atgtccccgg actgcttaaa acagcctatc
     1501 ctggtaggag tttggaagac gccgccagaa tcgagcggcg taacttcctc acccgcgcag
     1561 cctcttcttg gggatcaaag gttatcgtaa ccgctcacca tcgtgacgac ttgcttgaga
     1621 cctttctgat gcgtctgcta cgtgggagtg gattgtccgg atacgactgc ctaaaagcgg
     1681 tcgacccacc cttccacaga ccccttctca ctttctggaa ggccgatctg ctccgatacg
     1741 cccgagccca tcgtctcctc tactatgagg atcatacgaa cctagataac cgtttcctcc
     1801 gcaaccgcat acgaaatgag ctcgtaccct tcctgtcctc acgttttggc ccgcgtatcc
     1861 ccgatgtgtt gatccgggat caggaactgt tgtcttcggc ttctcaactc atcgatgaac
     1921 tgttagagcc gtatatacac tcggccgttt acacaaaaga ccgggtgaca ttttcctggg
     1981 aggacctgaa agaatactcc gatgcttttt tgtccgaact gttcgtccgg gcttgcaaac
     2041 gatggagagg gcacccgtac ggaatcgagg cgggaaaatt gacccgagcc atagaaagat
     2101 cgcggttggc cggtcacttc gttctaccct tccggcagga cctatggttc tttcgagaat
     2161 acgatctatg cggttttacg tctcagaaac cggagcgagt gtgcgctctc gagagggcgg
     2221 gctctgggtc cgacgaggga gtttgggaaa accccgatcg cccttttgag ttccgaaccc
     2281 aagatggtag aaaggtgacg ttcaccgctg agcgcggtat ccgccccgag cggatcgata
     2341 gtttgaaaac cgatcgttgg acggcgcttt ttgacgagga caaggtccag tttcccctga
     2401 cccttcgcgc accgatatcg ggggatcgct tccggccttt gggaatgagg gaggagaaaa
     2461 gactcgcccg tttctgctcg gaccaaaaaa tacccataag agaccgtcaa gaattactgc
     2521 tgttaatcaa tcggaaaagt gatataattt ggtgtatcgg aattcggata gcggataccg
     2581 taaagataac ccttgacacg cgtcacatat tgcgagtgcg gtgccaattg gaacccgaaa
     2641 agggaattga agggagggag cggagtattc cgcagttttt tgattaaaca tagaagagac
     2701 attacaggat tggaagagac cccacaagac ctcgttcttt ctttcgcatc gaattcggac
     2761 gacaaagatt ccaatcaaga tccgagaaga aatccacgct ttatgcccaa taaaaaaccg
     2821 ccgaggccga ccaaccaact gttttttttg ctgttgatga tttttttggt cgtgctgatc
     2881 ttttttgcct acaaaagccc gaatttttcc agcgttcagg ccgttgattt ttccaccttt
     2941 tggtcgatgg tcaaggatgg caaggttcgc gaagtggtga tagaggatac cggaaacatc
     3001 caattcaaaa cgaccaccgg tttccaggaa accctctatg agacctatgt gccttgggtg
     3061 atgaataacc cggatatcct cggggaactg atcgagcagg gagtgaagat aaaaagccag
     3121 aagagttcgg gcgattttat ttgggctttg ttgttaaacg ttcttccatt gttgcttttc
     3181 gtgttcatat ttttcgggat gatgcggtcg atgtccggga agaacaacca ggcgtttacc
     3241 ttcacgaaga gcccggcgcg aatggtttcc gcaagtaaaa aacgcgtcac cttcaaggat
     3301 gtcgccggtg tcgatgaagc ggtggaagac ttgcaagaac tcgtcatttt tctaaaaagc
     3361 ccgcagaaat tcgacaaaat gaaagcgcga atgccgaaag gggtcttact agtgggtcct
     3421 ccgggcacgg ggaagacgct tctggctagg gccgtcgccg gcgaagcgaa cgtcccgttc
     3481 tttcatatga gcgggtcgga cttcgtggag ctttttgtcg gggtcggggc ggcacgtgtg
     3541 agagacctct tcaaccaggc gaaaaacgct gcgccctcgg taattttcat cgatgaaata
     3601 gacgccgtcg gccgacatcg tggcgccggt ttgggaggcg gccacgatga acgcgagcaa
     3661 acgctcaacc aaattctggt ggagatggat gggttcgaaa tcaacgaagg agtcatcgtg
     3721 ctggccgcga cgaaccgacc ggatatcctg gaccccgcgt tgttacgccc cggacgcttt
     3781 gaccggaaag tcgtggtcga tccgccagat gtgagaggcc gagagtcgat tttgaacatc
     3841 tacctgaaga aacgcccgtt ggacccggaa atcgaggtgt ccgttttggc gcggcgcacc
     3901 cccggtttcg tgggcgccga cctggaaaac ctgtgcaatg aggccgcgtt attagctgtt
     3961 agagcgagta aggatttgat cgggatgtca gaactggagg aggcgatcga tcgggtcatc
     4021 gccggcccgg aaagaaaaag ccggatcatc agtccgaagg aaaaacgtat cattgcctat
     4081 catgaattag gtcacgcctt ggtgtccagc gcattaaaaa acgcagaccc ggtccatcgg
     4141 gtatcgatca taccgagggg ccactcttcg ctcggccaca cgctacaact tccgttggaa
     4201 gacaagttct tgatgagcaa gtccgagata tatgacaaaa ttagctcgat cttgggcgga
     4261 cgcgcggctg aagagatcgt ttttgacgag atcaccagcg gcgcttcaca cgacatagaa
     4321 cgggcaaccg aaatggcgag gtggatggtc tgtcaacttg ggatgagctc cgctctcggg
     4381 ccgatctcct ggggaaaaga ggagcaagag gtttttctag gacgagattt ctctcggatg
     4441 aaaaacttta gcgacgacac cgccaaagtg atagacgacg aagtgaaaaa aatcatcgag
     4501 aagtgctata atcgtgccaa agagataata cggaagtaca aaaacctcct tgatcgactc
     4561 gcggagatct tgctggaaaa agaggtcatc gacggtgagt tcttaaggga gatgctgtgc
     4621 acggagatgg caaaagaaaa ccataaacag ctcgaggcgc agatcgaggc tcagatcgag
     4681 ccgacgttga ttaaggaact ggggtagtgg gtccgagaaa acggaatgaa gaaaaacctg
     4741 tggctacttc ttttcgcgtt tgtggtgacg atcggctgcg gtatggccct atgggcgggg
     4801 ggcggcgttt tctacctgga agaatatccc caccccgcgc tcaacttcgc gattacggat
     4861 cggttctttt ctatcgaaca cagcggaaca tacatagacc atttcggatt tgcgaatttt
     4921 ctagatcttt atggtaagat tgactgggtg atcacaccta ttgtgggttt gggtacctgt
     4981 tgggggattt cttatgatga cctcctcttt atcgaaagag gcgggtttta tgttggaggg
     5041 ggtgttctct tgagctacgg tcgttactct cttcaattgg tcctcaaaca ctacattcct
     5101 ttttttcagg agatcgtttt ttctccttcg gtacaactca taggtagatt taagatagga
     5161 gagtggtggg agtgaaaaag tccgttcttt ttcttatcat gaccattttt gtgtttgttt
     5221 tctttacggg gtgtattaag cctaaccctg cttctagtat tacggttacg atcgacagtc
     5281 ctacctcgat cacggtcggg tctgcagcga acttcaccgt gaagctttcc tcgtcgaaat
     5341 tgggggggat cggcaatctc tttgcgaaag acccggtgat ttcaaaagtg acatggaact
     5401 ttacccatca gacggattcg agtaaaaact tccagtttac ggccacttct gcgacgacca
     5461 actatacttt cgggtctccc aatgtcttct tcacggggac gttcgtcctg tctgtctccg
     5521 ttgaagacaa agacggaaac agttaccagt cgaaatcgac caacaataag acggtcgaaa
     5581 tcaaagccag tacccctagt tttttgatcc tacctaagga tacaggtgat cacgaaatct
     5641 cgctgtcgga attgaagaaa aaccagatcg ttaagttcgt cctgtcggat ttgaccagtg
     5701 aaggcatctc aaatagccag attcagaact accagattcg ctgggcgctc tcgaaatggg
     5761 acgaagcgtc ctcgtcctac aaagagatcc ccgctctcgt gcaagaatac cgcatctatg
     5821 cgacctcgaa ctcctaccaa gtgccgatac cgcacacagg gaagctcgct ttgaaaatct
     5881 ctatcaagga tatcttcggg aacgtgacat ctttcgaaag agcgaagacc ttcgacgtgg
     5941 tgttcgcgat cccggaggcc cctcagctgc tggagcacga ctggacagga gacggtaaaa
     6001 agtacagact ggtattctcg aaatcggctg acgcttatta ttatgagatc tatcgtcaag
     6061 cggtttcgct ttctacagaa cgtattcaaa cctcgcaaat cgaagagttc atcggaaggg
     6121 cctttttgac cgacgccgaa aaggtcgtct ttgacgaccc gaaaccgacg aaaggttggg
     6181 tagtctattc ggtctacgcc atcgacggcg gtggggcctc cgcaaaaaca gaattctcta
     6241 tcaacgtggc gaacaggaag cccggaaaag cgaaactgat ctcaccgatc ggactagtga
     6301 cgtccgtacc cgatccagtc gcaggcttgt gggcttattg gtccgaaggt gaggacgtcg
     6361 atcccgggga tacggtcacc tacctggtct atgtgaaaga gggttccaat tttgttctca
     6421 ccaataggac aacttccaag agcgcggcga tcaactacct attcgccaac ggtatcgttt
     6481 attctatacg ggtggatgcc acagatggga tctccaccac aacgggagat gtcagcactt
     6541 tctggattat agacggttcc gtacaaccgc cgagaatcga agaagtcatt ccgctctact
     6601 tgaaggatcc ggcataccta tcgacgcgcg tgaccttctc ccacatcaac ccctatggcg
     6661 ccatgacagg aatcctaacc cgatacgtta tccagttctc caggtattcc actttcccgc
     6721 ccgccgacac ttttgagggc gaggttatag cccccggtac ccacacgtta aacataccaa
     6781 aacgttacgt gaaagagaat gaacccctat acgctcgggt tagagcgata gtgtctacca
     6841 atcacatcct gtttcaaagc aattggtcaa acgtggtgcg attctataaa tagccacact
     6901 atagttactc aaaagttccg gaggtgagga ccatcaacta cgatcaatac cagaaacagg
     6961 tgaaggaagc agcggaatac atcaaaaggt tcgtgaagga aagacccaaa ctcggaatcg
     7021 ttctcggttc tgggctcggg agtgtggctg ccgcgctcac cgatccattc acactaccct
     7081 acaaagaaat cccgcatttc cccgtttcta ccgcgccagg ccacaaaggg gagctggtct
     7141 tcggaaggat tggcgccctt caaacgctct tgatgagtgg gagactccac gcatacgaag
     7201 gcttttcgat gcgagagatc gtctttccta ttcgggtgat gcagctgctc ggcacggaga
     7261 tgctattcgt cacgaacgca tccggcggct tgaaccctga tttcgaactc ggcgtaccga
     7321 tgctgatcac cgatcacatc aatttcatgg ggaacaaccc tttagtcggg cagaactacg
     7381 atacctgggg tcctcgtttt ccggatatgt cggaggtcta cgatctcgag ctccgaaaga
     7441 aggcggtaga agccgccaga gaactcggga ttaagctgca ggaaggcgtt tatgtcgccg
     7501 tgaccggacc gaatttcgaa acgcccgcgg agttgcggat gcttcgccgt ttgggtgcgg
     7561 acgcggtcgg gatgtcgacc gttcccgaag tgatttgtgc ggtccacgcc ggaatacgcg
     7621 ttctaggcgt atccgcgata accgatcgcg cggttgcaga agacctggaa ccgctcacgg
     7681 ccgaacaggt gttggagatc gcagaaaaaa ccggacacaa aatcgcctcg atctttttga
     7741 aaatcaccga aaggttatag tcaacccaca ctgtgttgga atttattgtt ttaagcttgg
     7801 ttcaaggctt aacggaatgt ctcccggtgt ccagttctgg acaccttttt ttgttgagcc
     7861 gatggatcgg ctacgatctc tccctcgcga cggctgtagg attacatttg gccaccctat
     7921 tggccgttat cctctatttg cgcaaggaat gccggcagat cctggccggg atgttacaat
     7981 tcgataaggc ctattggcgg ctcttttggg ggttcttgct cgctagcgtg ccagccgctc
     8041 tcgtcgggtt tttggttaag gactctctcg agaccgcctt cgccaacccg acggcgatcg
     8101 ggataaacct gttgatcacc ggagggttct tgttcttgtc agacttcata aaacgagacc
     8161 aaactccctc tatgtcactg actcagatca cctggcggca agccttcgcg atcggattgt
     8221 tccaatcggt cgccgtttta cccggcatat ctcggagtgg gctcactttg gtgggctgtt
     8281 tggtcgtcgg tttacccagg gagttagcct ttcgttacac ttttctcctg tcgataccgg
     8341 tgattttggg gtcgggatta ttagaactcc gttctttttc tgtttcctca gaactcgtac
     8401 ccggatacgt catcgcgttt ttcatcgcgt tattggctct gttcctcttt cgaaaaccgg
     8461 ttctcaggag ccgattgcgg tatttcgcct attattgttg gattctcgga gcgcttcttc
     8521 ttctaatctt aaggtagaag cgcttcggcg acgtccaacc atcaaaacaa ccgcgaaggt
     8581 cgaacccctt cgcgtatttt tgtgctttga cggtattcct gtcagcccag tcccttctac
     8641 gaaatgggca gcgcgatccc ctcgctagtc tgcgccagcg ctcgggttag gccaacaagg
     8701 cgataacccg ttcccgagtc atatgatgca acgaatctaa agcagccggg tccaagcgca
     8761 aaacttccgc tctcaggctc ccaaaatcgg atttctccaa agtttcagtt tccacgaggg
     8821 ccggtttccc attcgtctca gaattctcga cgactcggtt tctcttgttc tcagtttttg
     8881 agatcagttc gccttgcggc atatttccgg ttagtcgttc gacccccata tcctcacctt
     8941 tctaatcttc ctttaggtcc tatgcttgat tataacacaa gcggaaagga ttattcaacc
     9001 cgagtgtgcg tcaagtttag gcactccgat atttttaccc acacgctttc gttttttagc
     9061 ggcttttcgt ggtatactta tgttagagac accttgaaag acacgggggt agaggaaatg
     9121 gtcgatttta gaaccgatcg aaaaaccgga atcctcttgc tatttttcct ctgttttgtc
     9181 tacgcttcgc tctttggagt tgcggagatc aaaattggca tctaccaaaa cagtccaaag
     9241 gtctttttga atgagcgggg tgagcctgcg ggatttttcg tggatatcgt gaacgaaatc
     9301 gtaaaaagcg tagggctaag ggtctcctac gttttcggga cgtgggcgga aaacatcgca
     9361 aagctcgact ggggagagct cgatatgctc gtggacgtct cttacacaga cgagcgggcc
     9421 gcgcgttttt cgttgaacag cataccgatc atcgaatcgt ggatccaggt cttttcgctc
     9481 cctgagacca cgatctttag ggctcaagat ctcgggggaa agagaatcgg cgccctctcg
     9541 gattccaccc agtaccgcta tttgagagac aaactcaaag aggatttctc gatcgattat
     9601 acgatcgaaa cgttttcaga ctacccccat caggttgagg cgctgatcgg aagaaaaatc
     9661 gatctatttt tagcggatcg gtttttttac ttttccaaag acaggccgca gaatgtgatt
     9721 ccaaaaccgg tcatcctgca acccaacggg gtctatttcg cgtttcgtaa aggatacgat
     9781 cctggcatcg tcggggcctt cgacaccgcg ttattctcta tgaaaaacga ttacggttct
     9841 ccctactacc gcgccttaaa ccaatggttc aacctatcga tcgagacgac ctttcccttt
     9901 tggctgaaaa cactgttggt ctctatttta gtcctaatga ctatgttgtc ggtgttcctc
     9961 ttccttcgag actaccgacg aacgaaagcc cataaccgag aattggaacg gcgggtttcg
    10021 gaacggaccg agcacctgaa tttggcttta catcaggccc agagtgcaaa tgaggcaaaa
    10081 agcgtgtttc tctcgagtat gagccacgag atacgaactc cgctgaacgc catcatcggg
    10141 ttcgctcaaa ttttaagacg agacgcgacg ctgacccgca agcaacaaga acaactgcag
    10201 acgatcgcgc agagcgggga gcatctcttg aaactgatca acaacatctt agatttctcg
    10261 aagatagaag cggggaaact gagtttggac ctctccgagt tcgacctcaa cacactgatg
    10321 gagtcactgg ttaacatctt ccggttacgt gcggaggaga aggggcttcg ctttttttac
    10381 gagccaaaat tgccggtgcc gtgctatgtg aggggcgatg aagggaaaat acggcaagtg
    10441 ttcatcaacc ttttagggaa cgcggacaag ttcacgcaga ccggcggaat cgtccttcgc
    10501 gcccggatca cccttcgaca agactccggc gagaggggct ccttcgcgga cttcgtcgca
    10561 gaagtggaag acacgggtcc ggggataccc gaagaagaag ttccgcggtt attcgagtcc
    10621 tttcattcac taaaagcggg gagggcctac ggaggaacag gcttgggtct atcgatcagc
    10681 aaaagattca tcgacttgat gggcggatcg atcacttacg aacaccgtcc tcggggaggg
    10741 agcatcttcc gattgcgtgt ctgtctcgac aaagtgaacg ccctcgccgc agccccccgt
    10801 tcggaggacc ggaccatcat cggattgaag ccgggcacgg ttcccccgag ggtattgatc
    10861 gtcgatgatc aagccgaaaa ccgagaactt ttgcgtgata tcctggaccc gatcggtttc
    10921 caaacacgcc ttgccgaaaa cggagaatcc gcgatagaaa tcttttcacg ttggtctcca
    10981 gatattgttt taatggatat gctaatgccg gtgatggacg gttacgaagc gaccaagcgc
    11041 ctaaaaagca ccgaagaagg tcggcaggtc ccgatcatcg ccgtcacagc cagcgctttc
    11101 ggagacgagg agaaaaaagt gttagagacg ggggtggacg ggtatctgcg taaacctttg
    11161 cggggggaag acctactcgc gctcctgagc gaacagttgg ggatcacgta cctatacgcc
    11221 cccacggcga atcaggatac gttgtccgat ccccccggtc aaccgaggcc gctcacggcc
    11281 gaagatttcg attttctatc cgacgacgag atagccgaaa tgcgaaaagc cttgtcaaac
    11341 ggagaaatgt tgagattgag agaattaatc gctcagatag gagaaaaacg gccgttcctc
    11401 gcggaaagac tgttggtatt ggccaagcag tataattacg aggacttgag ccgcctttta
    11461 gagcgataag gaggtcgagc taatgaaccc ggacttagaa ggggcttcga ttatgatcgt
    11521 agacgatacc ccggccaacc tagagctatt ggccgaaatg ctgcacgcca aaggataccg
    11581 ggtgttacaa tttccaaacg ggatgacggc gcttcacgcc tgcgtcaggg tgaaaccgga
    11641 tttaattctt ctcgatatta tgatgccgca gatggacggg tttgaggtga atcgccggtt
    11701 gaaaactgac gaaaaacttc gagacattcc ggtgatcttc atcagcgcct tggatgacac
    11761 cgcgaatatt gttcaagcgc tttcgcaagg gggtgtcgat tacatcacga aaccgtttaa
    11821 agaagaagag gttttggcac gggttcggac ccacttgaag atcgtgttcc tgcaacgcca
    11881 attgaaagcc cacaatgaaa atctcgaaga gttggtggca aagagaaccc aacaattgac
    11941 tcgggcccac gaaaggctga aggaattgga tcggctgaaa gacgatttcc tttcgatgat
    12001 ctcgcacgaa attcgcaccc ccgcgaacgg gctgcttgga gttggagaat tgcttataga
    12061 actctgcccg ccatcggaga atgcgcgcaa ctatgaggag atgttccgcg aatgcagcag
    12121 taggttacga aaactgatcg atgatgccag tttgctcggc gatatcgacc gccttattga
    12181 ggataccgat atagacgtat catttagcga tctcctgcaa gagatccggg aatcctttcc
    12241 tgaggtgagg atcacggtcg aaacccaagt ggatcccgaa aaggtgtttt tcaaagccga
    12301 cccggttctc ttaaaaaaag ccttcgaaac cacgctttta ttggcgagga agttctcgtc
    12361 caaaaaaaag cccgtctctc ttttaggatt ggcgggtgaa caacatctta gcgtccagat
    12421 tcccatagac ttgctatacc taactcatga acaggcggaa acgtttttta acatagaatc
    12481 caccttcaga gcttcttccc aagcggaatc tatggggctg gctcccgtcg tcgcgcacaa
    12541 gatactatcg gcgtttggtg gtggccttaa tctcgtgaag caggaaggaa caaaaggcta
    12601 tgtggaagcc atcatgctca cgcaagaaag aatcacctaa cgggagtcag cactttgcgc
    12661 cttgggaacg ccgaactttt aattcggcgt tcccagccgt gcggcgcttc gacagcgtta
    12721 ttcccttggg ttggggtata gaaaacaggc gacttcgtga ccttctcgaa cttccaagag
    12781 ggcgggggtc tccacccgac atcggtccat cgccttagag caacgcttta caaagcgaca
    12841 cccctccggt gggtcgatag gcgatgggat gtccccggcg atcagcatcc gatggccgga
    12901 tttccgctgg tgcggatccg gaacgggtat ggcctgcagc aacccttggg tgtatggatg
    12961 cagcggggct tcgaagagcc ggtccgtatc ggttttttct acgattttcc ctaagtacat
    13021 cacggcgatg cggtctgaga tgtgcttgac gacagaaagg tcgtgagcga tgaagagata
    13081 ggtcaaaccg aattcgctgc gtaggtcttc caacaggttg atgatctggg cttgcaccga
    13141 aacgtccaga gccgaaacgg gctcgtcgca gatgacgact ttcggtctca tcacgatcac
    13201 ccgcgcgata ccgatccttt ggcgttgacc accggaaaat tcgtgaggat accgcttcat
    13261 atactctggg agcaacccaa ctttctccat aatgtcggcg acggtattga tcatctctcg
    13321 tcccccgctc acgagcccgt tcgcttttaa tccttccgag atgatatcct ggaccgtcat
    13381 tcgagggtta agagaggcgt atgggtcctg aaaaatcatt tggatttggt gtcgtatcct
    13441 acgcatttgc gcattcttgt agcaaaaaaa atcttccatc gtttccgccg aatccccaac
    13501 ccgccaccaa cattgacccg atgtgggctc gtagatgcgc ataatcgtcc ggcccaccgt
    13561 agttttcccg caccccgatt cgccgacgat gcctaaggtc tctccggcgt ggagcgtaaa
    13621 agtcacgtcg tctacggcct tgagaaaaga tcttttacgc gagaacggga gtgatttgac
    13681 cgggaaatat ttctttaact tctcaactcg gagtatcgct gacgcagcca ccaggcacct
    13741 ccccttcttc tgcgtacaac cagcatttta ccctatgtcc cgattctatt tcgaatagag
    13801 ggggcggttc ctgcacgcat tttggaaacg ccttttcgca cctcgggtta aaccggcacc
    13861 cctttggaaa ggaagaggga tggggcacgt agccttcgat cacgtgaagc ttcccttgag
    13921 atttcttgtc gattgtcagt ttcgggatcg agttgatcaa gccacgggta tagggatgag
    13981 acgggtggtc gaatatttga aagatgccac ccgtttctag aatctgcccc gcgtacatca
    14041 cgatcacacg gccacacatt tcggcgacca cgccgagatc gtgcgttatg acgatcgtag
    14101 aggtattttg tgcggtcttc agttcgtgaa tgagttccag aatctgcgct tgtatcgtca
    14161 cgtccaaggc agtcgtcggt tcgtcggcga tgaggagcga cggtttgcag gcaagcgcca
    14221 tcgcgatcat cgcccgttga cgcatccctc cggatagcag gtgagggtag tcgtcgatgc
    14281 gttttttcgg ctccggtatc cgtaccatct ccaagagacc gatactcctt tgcttcgctt
    14341 ctttctccga tagaccttcg tgtaggcgat agacttcagc caattgctgc ccgatcgtga
    14401 aaagcgggtt aagagaggtc atcggctcct gaaagatcat agaaatcttt ttcccacgga
    14461 tcttctgcag ctcctccttg gaacactccg ccaagtcctt gccctcgaaa cagatacgtg
    14521 accgctctcc gaggatggcg tttttcggaa ggagtcggat gatagaaaac gcggtcacgg
    14581 tcttcccgca gcccgattct ccgacgatgc ccagtgtctc ccctcgatct agataaaaat
    14641 tgacctcatc caccggtttg acgcgaccga attgcgtttt aaaagatatt tctagattct
    14701 ctactttcag tatcctgtct tccatcgcta tctatccttt tcacgtatgg ctcgtaaatt
    14761 tcgggtctaa agcgtctcgc aagccgtctc ccagaaagtt gacgttcaag acggcaaaaa
    14821 agatcatcat accggggaag aaggtcaacc accacggggt gactcccata gccgttccga
    14881 ggatatagtt catcgacttt tggagcatat tgccccaaga gggcataggc ggttgtattc
    14941 ccaggccgag gtaactcagc gaggattcgg tcagaatcga gtacgacacg ttgagcgtcg
    15001 ccgacacgat gatcacgggc aagacgtttg gcagcacgtg tttgaacagg acggacaaat
    15061 tccttacgcc caccgctttc gccgaaacca cgtactcgga ttcacgtatc gtgagcgtca
    15121 gtcctcgaac gagacgggca actcccatcc aaccgaagac ggtcaacacc aatataatcc
    15181 ccgagattcc ggaaccgaaa accaaagtca acgtcaagag gatagggagc gtcggaatcg
    15241 agagcatgat atcgacgaaa cgcatcagca agcggtccag gatactgccg tagtaaccgg
    15301 aaaaaagacc tatggccgtc ccgatgatcg tggtcagcaa tgcggagaca aaacccacga
    15361 aaagggagac gcgtccgccg tacattagcc gtaccaatac gtcatgtccc agctcgtcag
    15421 tccctagaaa atgtccttcc gtgaagggcg gcgagaatat cgacccgaaa tccatatctt
    15481 cgtaactgac ggaggtaaac aggggaccta cgaaggaaaa gagaaccagc aggatcaaga
    15541 tgaccgctcc catcagcgcc aatcggtgcg ttttgaactt aatccaaacc agctttctat
    15601 aggtcacaat gttgagcgga gaagcggcct cttcgatctt ttcgactttt tttcgatacc
    15661 atcggaatga cttacccata acgcacccta agcctttctc gtaatacgta ttctcggatc
    15721 gatcaacaca tacagcaaat ccgcaacgag attggaaatg agcgtgatga tcgtcaagaa
    15781 catcaagcag ctgatcgcta agttgtaatc gctcccgatg accgcctgat acatcaaccg
    15841 gcccattccg ggccatgaga agatcgtttc tatgatcaaa gcaccgccga aaaagtaggg
    15901 gatgtcgagc gctatgatcg taacgatcgg aatcatcgcg tttttgaggg cgtgacgcag
    15961 gatgaccttc cgttccggcg cccctttggc gtaagctgtc cggacataat cttgatccag
    16021 gacttctaac atagacgtac ggatataccg cacccaagaa gcgacctgca cgaatcctaa
    16081 gacacaggcg ggcagaatga ggtgacgaac gctatcccaa aagacgcgcc attgaccggt
    16141 caggccgtag gtgctcatcc cagacacggg taaccacttc agctgaacgc tgaaaacgat
    16201 gatcgccatc agggcgaacc agaaactcgg tagcgatatc ccgataaaag cggcaacggt
    16261 cgccgtatag tcgaagaccg agtacttttt caaggccgac aaaatgccga tcggcatggc
    16321 gaccaccagc cccagtaacc aagaaacgac cgtcaaatat agcgtattcg gtagccttga
    16381 gaagatgagg tctttcaccg gttgcttgta catactcgag taaccaaaat cgccggtcag
    16441 catacttttc agccaagtta aatatcggat ggccaaaggg tcatcgaggt ggtagtactc
    16501 acgcaaggcc ttgagccgct ccggatcgtt cataaaggaa cgggggttct ccatacgcat
    16561 ttgcgtcagg ggatccccag gcatcaggtt gatcagcata aaaatgatga atgatatcgc
    16621 gaaaaatacc ggtatcaact ctaaaaagcg tttcaggaag tataccttca agctattact
    16681 ccttttgctg taagaccgcc atcgtgttca atcccgcgag catttgtacg gaaacaaggt
    16741 ctttgatcgg gatcgcgtga taccgcaagt gcttttcgag gaatgctatc gtcgtttcga
    16801 acatttcgtc cgccgtcttt ttcgcctttt ccaacacggc tctgtcgcct cccaagacga
    16861 tgttctcgtc gagaagtcgc cgtagcatgc ccaagctgag ggattcgtag aaacgggtcg
    16921 aataagtgtt atcgaacatc tccgcgacgg tggcaggatt cttcagagtt tcatctgttt
    16981 cgatccaatt ctccgttgcc ttgagacttc caggaacggt ttcgaggtag ttcgtgagag
    17041 cgtggtagaa aggggagtct ctatcctgaa tgacacgatc aatcgcgtcg aagatttgtt
    17101 tcagcgctgc gtaacgttcc ttcgagcgcc ctaacgactc tctcaaataa tgctgccggg
    17161 tttctgggag atgggtttga tcgttcacac gcgcgtcgta gtagtagggc atctcacaga
    17221 ccaaggtgaa agaatcggcg accgcgcccg catagtcgtc cgagctcgtg cccgcccgga
    17281 tgatcgtcgc gggatcggtg cccgggggca ggtgtttttc taaatagtcg tattcctctt
    17341 tcatcccaaa caattggaaa atcgctggcg ccagcttctt gaggaaaggc gcttcgggtt
    17401 cccccaagtt caacggaaga ttgaagagtc ccgggagctc ttggtatttt ttgaatagtc
    17461 cttctccaag gtcttttgtc acgtagtagt aaaccccccc gaaccccgaa ttgtgcaagg
    17521 aatacaagaa aatcggtttc ttctctttta tgagatccat caatacttga gtttctggaa
    17581 ggggtttgtt gaatttcaac gttttgtact ctatggggaa ggtccattcg acttgctggt
    17641 aaccggctgg tcggaaaaaa ttctccgcgt atttgcggat tgaagagcta tcggcaaacc
    17701 acttctcgtt gagtttcgtt ccgtctatgt ctatcgattt aatgaggtac cacgtgtaat
    17761 ccagttctct caatcgcggg tcatctttcg ccaatagttc gctcaacgcg tccaacatca
    17821 tcgcgccgat gggctcgttc ggatgcgggc acccgaagag cagggcg
//
LOCUS       DAOPXN010000028        17825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3219_length_17825_cov_5.076815, whole genome shotgun sequence.
ACCESSION   DAOPXN010000028 DAOPXN010000000
VERSION     DAOPXN010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 17825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17825
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_17825_cov_5.076815"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1916)
                     /locus_tag="PKM49_02740"
     CDS             complement(297..1916)
                     /locus_tag="PKM49_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HNY81764.1"
                     /translation="MRKLFLLLAVLLVAVLGFSEIVTLTILQTSDIHGNVYPINYATN
                     KPADSGLAKIQTLVKQLRSKDPDLILIDNGDLTQGTPFTYYHIRKEPTAPNPMIQVLN
                     LMGYHAGVVGNHEFNYGMGLLSKTAAEANFPILSANIVRRFTKTPYFEPYRVLTVKGV
                     RVGILGLTTKYIPNWEDVRNVLDVDFLDPVETAKVYVPILREKEKVDVVIVSYHGGFE
                     RDIVTGLPTEELSGENQGYQLLQEVKGIDLFLTGHQHRTIVGFCQGVPVLQPSNWGQL
                     VGKAEIKLDNSDKRWIIVSKSVELLSVKGVPADEEVLALVRDVEEKTQKWLDTPIGVA
                     KGDFSVVDPLITRMGDNALLEFINKVQMNISGAKISSTALFTNDIKGWGSGPVTLRDV
                     NAVYIYANTLKVIRVTGQDIKGALERCADYFTIVDGKIAVNDTWVYPKPQHYNYDVWE
                     GIDYTLDISKPVGQRVVKLNYEGKPMAMDKEYDVVLNNYRAGGGGNYLMFQGKPVVKD
                     IMMEVAELMSDYILERGEIEATVDGNWKVIH"
     gene            complement(2015..3139)
                     /locus_tag="PKM49_02745"
     CDS             complement(2015..3139)
                     /locus_tag="PKM49_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81765.1"
                     /translation="MPASSVSLTNVYKAFKGFKGEEILAVDDVSFRIEPGAFVTLLGP
                     SGCGKTTSLRMIAGFENPTKGKIKIDEEDVTFIPPWKRDTAMVFQSYGLFPHMNVGDN
                     VGYGLKMRKVTTSERHKKVSAILDLVGLSGFEERPPSSLSGGQQQRVALARALVVEPS
                     VLLFDEPLSNLDVLLREQMRVEIRRIQQKTGITAIYVTHDRTEAMTLSDTVIVMNKGK
                     IVQTGTPFDIYEEPNSIFVADFMGKMAYFPVKILAWEGKDAIVELYDKKLSVRVAKNV
                     TPNDSQRYILACRPESMKMQENGKGVVPGVITTNVYLGNAIESYIKTEFGEVMVKSEL
                     PKEGLRPEGSPVSLSIREDKAVLLPLDPGIDGLSLNKANI"
     gene            complement(3144..5339)
                     /locus_tag="PKM49_02750"
     CDS             complement(3144..5339)
                     /locus_tag="PKM49_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNY81766.1"
                     /translation="MKVKWVKAILFCVLGIILIVWVFRTLETEFTNISIQRLKEAGLL
                     LSQSVPSRSYGQWISSTREQYPDILPFISEGNAEFGEKVRPVISSKEDQAFFDWAQSL
                     PFYAYGLESTYYEEVYFYPKRIEWKGHPVYISFTPIIPEEVGYVTGTLILLHDGSVIR
                     NYGNLLKGFAITFIVLLSCILFVNAFSKDPILVYTILIIFLIVGIFIAFPLYQAFRLT
                     VMKDGVFTLEVWRHVLSNRNYLQALWGSILLGVLTATVSTAIGFLFAFTLTRTNVRGK
                     GFYATMATLPVISPPFSLTLSLILLFGSYGLITRRILGLTNVDIYGLDGLVIVQTMGM
                     FPIAFMVLSGVLEAIDSTFEEAAMNLKATRWKVFSTVTLPLSFPGIASAWLLVFTNSI
                     ADFANPLILAGKYKVLSVEAYLEVTGMNHLDIGATLSVLLLIPTFLAFGLQRYWISKK
                     SYTTVTGKPTGRILEVVSPNVKKVLVALMVAISGFLLSLYATIVAGCFVKNWGIDYTF
                     TFDNVVEALQRGKDAIGDTTLLAAIATPIAGLLAMIVAMIIVRKRFPGKGLMQAMVIT
                     PYAVPGTLVGISFIIAFNKPPLLLVGTAAIIIINYVVRELPVGVESGIASLKQIDPSI
                     EEAAQSLGADTATVFRTIVLPLIRPAFISSLSYTFVRSMTAVSAVIFLISAKWYHMTI
                     LIYNFSENLRFGLASVLATTLMVIVLTVFWVLRFLVGKSSSLQKNIIVR"
     gene            complement(5447..6433)
                     /locus_tag="PKM49_02755"
     CDS             complement(5447..6433)
                     /locus_tag="PKM49_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81767.1"
                     /translation="MKRLTTIALCLLLTALVFSANSVKAYTTFEEPLAKEVFDAFEKE
                     TGIRVEWVRLSTGEAVARLEAERENPQASIWVGGVGVLHVQAKMKGLTASYKSPQFLS
                     IPEQYRDPEGFWVGLYVGPLAFVTNKNRAKELGISAPTNWDEIASETYRGMVRMADPI
                     TSGTSYNVITTIRYLYEGDEERTFEYLKRLDKNVNMYTRSGSAPGKECAIGETTVAIG
                     YLHDLVRLVKNGAPLEITLPSDGTGFEIAAMSLVKGGKDPVEAKKLYNWVLGPAAQGI
                     IAAWYVIPLSPEAPTDDIYIQIQDIKTVEQDLVWDAENRDRLTARWKAEIGR"
     gene            7492..8520
                     /locus_tag="PKM49_02760"
     CDS             7492..8520
                     /locus_tag="PKM49_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNY81768.1"
                     /translation="MTRTNAYVITEPFRGQVRGFDLLDIASDDALVKVRACGVCTTDR
                     RLFAGTLKVPYPLIGGHEVAGEVVAVGKDANGLEPGARVALDTIYRCGSCFFCRKGLD
                     HLCLYSKKETKLEGVSLVAGGFSEYLIVKQTQLFLVADTVDFAEAALSEPLACCLHSV
                     QRARLSFGDSALIIGAGTMGLLHAFVATLQGLTPLISDSDAERRCFANRLGFQTVEPS
                     RVNEEKHRVNEGLGFDGVFITAPVLSLINDSLSYVRKGGAIVLYTSVHPNGTVAFDAN
                     QLHYSEAYLTGTEGRTRADFQKAVTLISKGLIDLKPLVTKRIQLDDLSEELSTMPSGN
                     SQRTVVTF"
     gene            8536..9324
                     /locus_tag="PKM49_02765"
     CDS             8536..9324
                     /locus_tag="PKM49_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81769.1"
                     /translation="MALFTMKRMRRLFRKDGRSVIVALDHGAAMNVLPDLKDTPSVLR
                     AIVENGADAVLTSFGILKDYWEILEDTPVILRLDGGITSFGTGTKRYSQLYTVEDALR
                     LGADGVACMGLPGCDYENDTLSYLAGICAEARRWNLPVLAEMLPGGFGHTARAPEHLA
                     FAARVGVELGADIIKTEYSGNPDDFKEMVEGVKKPVVVLGGAKSERYEEIFQRIRSAI
                     DAGASGIAVGRNIWGYKEPGKMTAALSAIVHGQASVESALHMLQ"
     gene            9360..10346
                     /locus_tag="PKM49_02770"
     CDS             9360..10346
                     /locus_tag="PKM49_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY81770.1"
                     /translation="MTKRRMAIVVALISIVSCFSFSVALMVTGEIGGNPIYEMMVSGA
                     ESASAELGFELKTVEGGYNSAKWESNLIALAATGKYDLIVTFTEGMPKSVEKAADMFP
                     RQKFALIDGIAAIKPNVYSLGFKDEEMCYLAGYFAGLITLSDLPGANPNLTVGLIGGD
                     TYPAMTDRMKPAFTKGARAVHPDIDVLFSVVGSWSDPKKGKDLAQAQYAQGADIILTI
                     AGGSGVGAIQEALRLNKYIIGVDSNTIPLAPGTILACALKRADLAMNDVMRRAFSSAL
                     PYGATERHGIAEGVIGFTFDDPTYLETIPEPIRKQMLEVFDALKNGVIDPLS"
     gene            10415..12019
                     /locus_tag="PKM49_02775"
     CDS             10415..12019
                     /locus_tag="PKM49_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81771.1"
                     /translation="MKTVGPYAIEMEGISKVFETNRTVALDRARFRVSKGAIHALVGE
                     NGAGKSTLMKILCGLERKNAGEILVDGIPKEIRSVKEAYANGIGMVNQQFHLIEDYTV
                     LENILLGVEKTNRWGLIDRRESAKEVQKLIEMSRLELPAGRKVSELTMGQRQLVEILR
                     ILNRAVEILILDEPTSVLSPREIDWLFSIIRELRARGKTIIFISHRLDEVFELCDSIT
                     ILRNGRDIAEGTLSDFDKKQVSFLMIGEPLGALNPKRSPKQCPVLLRAENLSVRDADR
                     KTEVVRSVNMMLRGHEIAALVGVSNNGKQELVEALSGLRETSSGNIYLKDKKMTGSTP
                     RGFRKAGIAFIPEDRVRMGASVSSSVLDNILATKYFEAPYTKRGWINRKEAGKTAESL
                     IREYRIKGASVHAPLATLSGGNIQRVILAREISSDPSVLILCEPTWGLDHRSCDHIYE
                     RLYELAESGKAVLIVSSYLDEALRLANRIFVIYKGEIIAELENSSSLTKIAIGELMLG
                     LGRPLSQGYEGDGSSIKAGSFDGHTS"
     gene            12003..13064
                     /locus_tag="PKM49_02780"
     CDS             12003..13064
                     /locus_tag="PKM49_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81772.1"
                     /translation="MVTLRDLWRTAFIVGAALGFGLCVILLASERPGDAIYAFFLGPF
                     SNAYFFGNLLASAIPLILTGAAACVSFSASVFNLGLEGQVYLGSFLGVFLTCRFGELP
                     PLVVYPLVFAIVFCCSGTLAALSGYLKRARNVNVLISTLLISYITIYVVDALIETVFI
                     DPTAGLAATPYIHDKFLFARLLPPSDLHSGLFLALALTIILRIIMKKSALGFEITLTG
                     KSPVFARYAGVSVSKVLTLSLFISGGFAGIAGITDIYGIHGRMFRGFSSGYGWNGIAV
                     ALIAKNHPLWVIPAALFFAYLENGANVGALLADITPEMARTIQATIFFTVTADALFSL
                     IRKNKGGDAWTPSSTVSSL"
     gene            13031..13912
                     /locus_tag="PKM49_02785"
     CDS             13031..13912
                     /locus_tag="PKM49_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81773.1"
                     /translation="MDTFINSIVPMMTPVLFAALGGLFTHLSGLLNIGLEGLMLLSAF
                     FSIYVAHLTHNLFFAVLAGIGISMLLAVFAGFLSLNLRANIFVVGLGVNLFANGFTVM
                     LSSLLFSTKGTVFFQNLPKLSYIEIPLVHRIPYLGPILSGYGALDYLAIGAAVLCAII
                     LYKTPYGYHLRAVGRDEKAAQNAGISVYWHRLYALALSGLFAGLGGCALALPLQTFVG
                     GMTNGRGWIALVAVILGGGKPSGVFIASLIFGAASALSNWLQASAPMSPKLMMTLPFL
                     ITLLFMIVKKNNTEEGV"
     gene            13905..14735
                     /locus_tag="PKM49_02790"
     CDS             13905..14735
                     /locus_tag="PKM49_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNY81774.1"
                     /translation="MFSIDSFFLALKERLPHFGPAQRKIAEFIIRNPRVIDGLTIREL
                     AEKTGTTPAAVVTFYKSLSYNGFRAMKSALSEEIDLVESMSFYLVRPSNHHTDLISAI
                     HNNVRFASASLSQESLRQACDLLLKSKAIDILAFGFDAVAGQDAFLKLKQLGFQCNFF
                     QNACLQKLSASHLNTEGCALAISSSFSSRDILDSIQFARRRGAKTIAFAPQESQVLEA
                     ADVRLPLQSKSEILAEGGILSRYVQLFAIDSLFLKLLESDPLRFAQTFEQFDRIIHHK
                     "
     gene            14748..16190
                     /locus_tag="PKM49_02795"
     CDS             14748..16190
                     /locus_tag="PKM49_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNY81775.1"
                     /translation="MADLFGTIDIGSTNCKIGIFDEEANFLYRDDSPISSQLLRRSGT
                     IDPFEIWETVSGLLGRLPERIKRRLRALCVIGQGPTVVPVDRSGQPLYRAITWLDGRG
                     KERIPVLIDQGVDPQLAAGLSALEWLKEELSEQAVLLQPADYVAYRLTGRLANMSFPF
                     VGYLPWSRQALEEREWSHAFLVPEIVEPGSLFSNLTQEAADRFNLSPQTLLVAGAPDF
                     VAALVGTGTIEDGSLCDRGGSSQGVTLCSKSEVFPPGLMTTPFFKPGLWKISAIMNTT
                     GIALEWFCRKVTGQPLELTLEQAVGCATPTKILFLPYLSGERSPHWDSKAKGVFWGLT
                     LDSDARRMTVAILESVGYAIADAIERMENAGCVIKRIRTTGKQALSPIWTQIKSDILG
                     RPVEVPEIRESELWGGALFSMKAVSNRDLTFLSHEKTRVGQTYYPDSKKHLLYKELLS
                     MFQSLYPANKPLFAQWARIDFARGPTTQRD"
     gene            16844..>17825
                     /locus_tag="PKM49_02800"
     CDS             16844..>17825
                     /locus_tag="PKM49_02800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY81776.1"
                     /translation="MRRILLLICFVFCWLCGSVFSMTQAELEGYLALNYRNIVYVFDP
                     QTLNILFSNERASEYYGYTLEQFTSMNLMDISLLSEDDLRDQINEALEVKQKPIINSQ
                     RLADGSIRTIEVHGWPIEVDGKTLLFTTVFDVTERERTKKELEKSLVKLTKAEEMGDV
                     GHYEIYLSEEKMVLSKGAEMIYGLVGDLWPSSTIYELILPEDREKVAKSREELVRVGK
                     RYDVTFSIRRANDGELRYLRSLGDYDTVNNTIFGVVHDITEQKKAAAELKARNDAFFW
                     TLTAGILTITAIAALLALSVLKQLKVGRELLESKRKIDVQNEELQAANEEL"
BASE COUNT         4117 a         4743 c         4754 g         4211 t
ORIGIN      
        1 tctatgttct ctcggtgtcc tccgcgctct ctgtgcgaac cctgtttttt attgtaaaac
       61 atgccaactt aaaagttggc gttcccagcg tatcgcgccg tcgcgaaaag aaggggcgct
      121 tacgccgcga tgctgagctc gggaacactc ccagtgaaaa gacaagaccc tgccaaacta
      181 aagtttggcg ttcccagggc gctcccagaa aggcaatccc agaaaggtgg tcgcttacgt
      241 gtaccaaaaa aaaccgcgcc ttacggcgcg gtttcctttc gacttttcca ttcaggctaa
      301 tggatgacct tccagttgcc gtcgacggtg gcttcgatct ctcctctttc gaggatgtaa
      361 tcggacatca actccgccac ttccatcatg atgtctttca cgacgggttt tccttggaac
      421 atcagatagt tcccgccgcc tccggcgcgg taattgttca ggacgacgtc gtactctttg
      481 tccatcgcca taggtttacc ttcgtaattc agtttcacga cccgttggcc aacgggcttg
      541 gaaatgtcta gcgtgtaatc gatgccttcc cacacgtcat agttgtagtg ttggggcttt
      601 ggataaaccc atgtgtcgtt caccgcgatt ttcccgtcta caatcgtgaa atagtcggcg
      661 cagcgctcaa gggcgccttt gatgtcctga ccggttactc ggatgacttt gagcgtgttg
      721 gcgtatatat acacggcgtt gacgtctctc agagtcacag gtcccgaacc ccagcctttg
      781 atgtcgttcg tgaacagcgc cgtagaagag atttttgcgc cggagatatt catctgcact
      841 ttgttgatga attccagcag cgcattgtcc cccattcgtg taatcagtgg gtcaaccacg
      901 gagaaatcgc ctttcgcgac tcctatcggc gtatccagcc atttctgagt cttctcttcc
      961 acatcgcgta ctaaggccaa gacctcttcg tcagccggga cgcctttcac ggagagcagt
     1021 tcaacggact tcgacacgat gatccaccgt ttgtccgagt tgtccagctt gatctccgcc
     1081 ttcccaacca gctggcccca attcgacggc tggagaacgg gtacgccttg gcagaatccg
     1141 acgatggtgc ggtgttggtg accggtcaag aacagatcga tgcccttcac ctcttgtagc
     1201 agctgatagc cctgattctc cccactcagt tcttcggtcg gtaggccggt tacgatatcc
     1261 ctttcaaacc cgccgtggta agaaacgatc accacatcca ccttttcttt ttctcgtaaa
     1321 atggggacgt acacctttgc cgtctcgact gggtccaaaa agtccacatc taaaacgttt
     1381 cgcacatctt cccagttggg gatgtacttt gtggttaacc ccaagatacc gacgcgcacg
     1441 cctttgacgg tgagcacacg atagggttcg aagtaaggag tttttgtgaa tctgcggaca
     1501 atgttcgcgc tgagaatcgg gaaattggct tccgcggcgg ttttgcttaa taggcccatc
     1561 ccgtagttaa actcgtggtt tccgaccaca cctgcgtgat atcccatcag gttcaacacc
     1621 tgtatcatcg ggttgggagc tgtcggctct tttctgatat ggtaataggt gaacggggtg
     1681 ccctgggtca aatcgccgtt atcgatcagg atcagatccg ggtctttgct cctcagctgt
     1741 ttgacgaggg tttggatttt cgctaaccca ctatcagccg gcttattcgt cgcatagttg
     1801 atcggataga cgttcccgtg gatatcactc gtttgcaaaa tcgtaagcgt cacgatttcg
     1861 gaaaaaccga gtacggcaac cagtaaaacg gctaacaaca agaacagttt tctcatagaa
     1921 tccctcctta tgtgttcgat cgatccaaat actcgtttct ctctatcggt cgatcgtcgc
     1981 tttctttcgc ttctctttca agctgatgat ctctctatat gttggctttg ttcagagaaa
     2041 gcccatcgat ccccggatcc aagggcaaca agaccgcctt atcttcgcga atggaaaggc
     2101 tgaccggcga tccttcgggg cgcaggcctt ccttcggtaa ttcggatttg accataactt
     2161 ccccgaactc cgttttgata taggattcga tagcgttccc gaggtaaaca ttcgtcgtga
     2221 tcactcccgg gacgacacct tttccgttct cctgcatctt catcgactcg ggccggcagg
     2281 ccaagatata gcgttgggaa tcgttcggcg tcacattctt cgccacacgg acagaaagct
     2341 ttttatcgta tagctcgacg atcgcgtcct tcccttccca agccagtatt tttaccggaa
     2401 aataagccat cttgcccata aaatcggcga caaatatcga attcggctct tcatagatat
     2461 cgaaaggggt gcccgtctgg acaattttcc ctttgttcat cacgatgacc gtatccgata
     2521 acgtcatcgc ttccgtgcgg tcatgggtca cgtagatcgc ggtgatgccc gttttctgct
     2581 ggatacgtcg gatttccacc cgcatctgtt cgcgtagaag cacatccaag ttggataagg
     2641 gctcgtcaaa cagcagaacc gaaggttcca ccaccagcgc gcgcgccaga gccacccgct
     2701 gttgctgacc tcccgaaagg ctggaagggg gccgctcctc aaaaccgctc aatccgacga
     2761 ggtccagaat ggcgctcact ttcttgtgtc gttctgaagt cgtgactttc cgcattttca
     2821 ggccgtaacc cacattatct ccgacgttca tatgcggaaa caacccgtag ctttgaaaaa
     2881 ccatcgccgt atcccttttc cacggcggga tgaaagtcac gtcctcctcg tcgatcttga
     2941 tcttcccctt ggtcggattc tcaaaaccgg cgatcatcct caagctcgtc gttttcccac
     3001 aaccggaagg gccgagtaag gtgacgaacg cgccgggttc tatccggaac gagacgtcgt
     3061 cgacggccag gatctcttcc cccttgaagc ctttgaatgc cttataaaca ttggttaaag
     3121 aaaccgaaga ggcgggcaca ctatcacctc actatgatgt tcttctgaag gctggaagac
     3181 ttccccacca aaaaccgcaa tacccaaaag acagtcaaga caatgaccat caacgtggtg
     3241 gccagaacgc tagccaaccc gaaacgcagg ttctccgaga aattgtagat gaggatcgtc
     3301 atatgatacc actttgccga tatcaggaaa atgaccgcgc tgacggccgt catcgagcgg
     3361 acgaaggtgt aggaaaggct ggagatgaag gcaggccgta tgagaggtag gacgatcgtg
     3421 cgaaagaccg tcgccgtgtc cgctcccagc gactgcgcgg cttcctcgat agacgggtcg
     3481 atctgcttca agctcgcgat gccgctttcc acacccaccg gcagctcccg gacgacgtag
     3541 ttgatgatga tgatcgccgc cgtccctacg agaagcagcg ggggtttgtt gaacgcgatg
     3601 atgaaactga ttcctaccag cgttccggga accgcgtagg gtgtgatcac catggcctgc
     3661 atcaaaccct tcccaggaaa tcgcttcctc acgatgatca ttgcgacgat catcgccaat
     3721 aacccggcga taggggtggc gatcgcagcc aacagcgtcg tgtctccgat agcgtccttc
     3781 ccgcgttgca gcgcttcgac gacgttgtcg aaagtgaacg tgtaatcaat cccccagttc
     3841 ttgacgaaac agcccgctac gatcgtcgcg tagagcgaaa gtaaaaaacc ggatatcgcc
     3901 accatcaacg cgacgaggac ctttttcacg ttcgggctga cgacttcgag tatccggccg
     3961 gttggcttcc cggtaacggt tgtatacgat tttttactga tccaataccg ctgcaggccg
     4021 aaggcgagaa aggtagggat gagcaagagg accgacaggg tcgccccgat gtccaggtgg
     4081 ttcatcccag tcacttccag ataggcctcg accgacagaa ccttgtactt cccagctaag
     4141 atcaacggat tcgcaaaatc cgcgatcgag tttgtaaaaa cgagtagcca agcgctcgcg
     4201 ataccgggaa aggacaaggg gagcgtcacg gtcgaaaaga ccttccagcg cgtggctttc
     4261 aggttcatcg ccgcttcctc gaaagtcgag tcgatcgcct cgagaacgcc actgaggacc
     4321 ataaaggcga taggaaacat ccccattgtt tggacgatca ccaatccgtc caatccgtag
     4381 atatcgacgt tcgtcaatcc caggatcctt ctcgtgatca aaccatagct accgaagagg
     4441 aggatcaagg ataaggtcaa agagaatgga ggagagatca cggggagagt cgccatcgtc
     4501 gcgtagaagc ccttccctct gacattcgta cgggttagcg taaaggcaaa caaaaaaccg
     4561 atcgcggtcg acaccgtcgc tgtcaaaacg cccagcagaa tgcttcccca cagcgcctgt
     4621 aaatagttgc gattactcag aacgtgccgc catacttcca gtgtaaatac cccatccttc
     4681 atcaccgtca aacgaaaggc ctgatacaaa gggaaagcga tgaatatccc gacgatgaga
     4741 aagatgatca ggatggtata gaccagaatc gggtctttgc taaaagcgtt gacgaacaag
     4801 atacaagata gcaagacgat gaaggtgatg gcaaacccct tcaagagatt cccgtagtta
     4861 cggataacgc ttccgtcgtg taggagtatg agcgtgcctg taacgtaacc tacctcttcc
     4921 ggtatgatcg gcgtgaaaga aatataaacc ggatgtcctt tccattcgat ccgtttggga
     4981 tagaaataga cctcttcgta gtaggtgctt tccaatccgt acgcatagaa gggcaaggat
     5041 tgcgcccaat cgaagaaggc ttggtcttct ttcgacgaga taacgggacg gactttctct
     5101 ccgaattccg cgttcccttc ggagatgaag ggaaggatat ccgggtattg ctctcgcgtg
     5161 gacgatatcc attggccata agaacgcgaa ggtaccgatt gggaaagaag cagccccgcc
     5221 tctttcaacc tctggataga aatattcgtg aactccgtct ctaaggtgcg aaatacccac
     5281 acgatcagga tgatacctag cacacaaaaa agaatcgctt tcacccattt gaccttcaac
     5341 gatctacccc ctccgcagtc cgccgatggc tccaaaggcc gaagccttta gagaagaacg
     5401 gagaaagggc ctttggatac cctttctccg aggttaatcg attgccttac cgtccgattt
     5461 cggccttcca cctggctgtg agccggtctc ggttttcagc gtcccaaacg agatcctgct
     5521 ctacggtttt aatatcttga atttggatgt aaatatcgtc ggtgggggct tcaggggaga
     5581 gtgggatcac gtaccaagcc gcgatgatgc cctgcgcagc ggggcctaga acccaattat
     5641 agagtttttt cgcctcaacg ggatccttcc cgcccttgac caaagacatc gctgcgatct
     5701 cgaaaccggt gccatccgaa ggcaaggtga tttcgagcgg cgcgccgttt ttcactaggc
     5761 gaaccaggtc gtgcaaatag ccgatcgcca cggtcgtttc accgatcgcg cattctttcc
     5821 ctggagccga cccggagcgg gtgtacatat tcacgttctt gtccaagcgt ttcaaatact
     5881 cgaaagtcct ttcttcatca ccctcataca gatagcgaat cgtggtgatg acgttgtaag
     5941 aggtgcccga ggtgatgggg tccgccattc ggaccatacc gcggtaggtc tccgacgcga
     6001 tctcgtccca attcgtaggc gccgagatgc cgagttcttt cgcacggttt ttgttcgtca
     6061 cgaaggcgag aggcccaacg taaagaccca cccagaaacc ctccggatcc cggtactgct
     6121 cggggatact caagaattgc gggctcttgt aactcgcggt caagcctttc atcttggctt
     6181 gcacgtgcaa cacacccacg ccgccgaccc atatcgaggc ttgagggttc tctctttcgg
     6241 cttccaaacg agcgaccgct tcgccggttg acagccggac ccactccacc cggatcccgg
     6301 tttctttttc gaaggcgtcg aagacttctt tcgcgagcgg ttcttcgaac gtggtgtacg
     6361 ctttgacaga attggcagaa aagactagtg cggtgagtag aagacacaag gcaatcgtag
     6421 tgaggcgttt cataattttc ctccttctct atagtgtgta aagatgcgtt aaggcaaaga
     6481 cgattataac acaagtgact ttttttagta gcccgtatgt gggtataata tcgaggcgca
     6541 acaaaatggg aaccccgaac tttagttcgg caagtttcat tgcttttccg taagctcgcg
     6601 agagtcatta aacgcgtacg ccgcgatggg tctgtgaggt tatcgcgggg taagtggttt
     6661 ttacgacccc gcgatccgcc ttttgtcttt tttacaccgc cccgggagcg ccctgggaac
     6721 gccgaacttt agttcggcat gtttctttgc ttttctgtaa gctcccgaga gccattaaac
     6781 gcgtacgccg cgataggtct gtggggttat cgcggggtaa gtggttttta cgaccccgcg
     6841 atccgcccta ttcccttctc aaaagcgtat cgcgcggtcg cataaagaaa aagcgcttac
     6901 gtcgcgatga ccgtagaaac cccgcagtcc gtcctattcc cttctcaaaa gcgtatcgcg
     6961 cggtcgcata aagaaaaagc gcttacgtcg cgatgaccgt agaaaccccg cagtccgtcc
     7021 tattcccttc tcaaaagcgt atcgcgcggt cgcataaaga aaaagcgctt acgccgcgat
     7081 gctgcgctcg ggaacactcc cagtgaaaag agaaaaccat gccaaactaa agtttggcgt
     7141 tcccagaacg gatcgcgggg tcccgttggt taccccgcga taacccacga agttcggcgt
     7201 tcccagccca cgaaggctcg tgacccgcct tttgtctttt tttcgccgcg ttgaccacat
     7261 agaataaaaa tcgtacggtc gattctgact ttgtttcttg atcgcgcctc ataacttgac
     7321 agacacgccc ccttctatag agaggaaata tagagtataa tctatcgtct tccaacaaaa
     7381 aaagtttccg ggagtcaagc cgtatctgtg aagtgagaag acagcaatct acctgcttct
     7441 tagatacgcg cggggctagg agtcatgagc cggatattga gaggagcgcg gatgacgagg
     7501 acgaacgcgt acgtaatcac tgagcccttc cgcggacaag tgcggggttt tgacctgttg
     7561 gatattgctt ccgacgacgc gctggttaag gtacgcgctt gtggggtttg caccacagac
     7621 agaaggttat tcgccgggac gctcaaggtt ccttatcccc tcataggcgg gcacgaggtt
     7681 gcgggcgaag tggtagcggt ggggaaagat gcgaatgggc tggagcccgg ggcgcgtgtc
     7741 gcgctagaca cgatctatcg ctgcgggtct tgtttcttct gccggaaagg gttggatcat
     7801 ctctgtctct attcaaaaaa agagacgaag ctagagggcg tatccctcgt tgcgggcgga
     7861 ttttccgaat acctcatcgt caaacaaacg cagctcttcc tcgtcgccga tacagttgat
     7921 tttgccgaag cggcattgtc cgagcccttg gcctgctgtt tacacagtgt tcaaagagct
     7981 cgcctgagtt ttggcgactc ggctctcatt attggcgcgg gtacgatggg actcttgcac
     8041 gccttcgtcg cgactctcca agggctgact ccattgatca gcgattcgga cgcggaacgc
     8101 cgctgtttcg ccaaccggtt gggctttcag accgttgagc cctcgcgggt caacgaggag
     8161 aagcataggg tcaacgaggg cttgggattc gatggggttt tcattacggc gcccgttctg
     8221 tctttgatca acgactctct atcctacgtt cgtaagggag gggccatcgt tctctacacc
     8281 tccgttcatc ccaacgggac ggtcgcgttc gacgccaacc agctgcatta ttccgaagcg
     8341 tatctcacgg ggacagaagg gagaaccaga gcggacttcc aaaaagcggt cacgctgatc
     8401 tcgaagggct taatcgatct caagcctttg gtgacgaaaa ggatccaatt ggatgattta
     8461 tcagaggagc tgtccactat gccctccgga aacagccaaa gaacggtcgt cactttttaa
     8521 tttggaggtt atacaatggc actattcacg atgaaacgga tgcgccgact ttttcgaaag
     8581 gacggccgga gcgtaatcgt cgcgctcgat cacggcgcag cgatgaacgt tctccccgat
     8641 ctcaaagaca cgccttcggt tctgcgcgcc atcgtcgaaa acggcgccga cgccgtctta
     8701 acctctttcg gcatcctgaa ggattattgg gaaatcctgg aagacactcc cgtcatcctg
     8761 cggttggacg ggggaatcac ctctttcggt acgggaacca agcggtatag ccagctctac
     8821 acggtggagg acgccttaag gcttggggcg gatggagtgg cgtgtatggg attgccggga
     8881 tgcgattacg agaacgacac gctgtcttac ctcgcgggaa tatgcgccga ggcgcgccga
     8941 tggaaccttc ccgtattggc ggagatgctg cccggaggct tcggccacac agcgcgtgcg
     9001 cccgaacacc tcgccttcgc tgctcgagta ggagtggaat tgggcgccga tatcatcaag
     9061 accgagtatt cgggaaaccc ggacgacttc aaagagatgg tggaaggtgt gaaaaaaccg
     9121 gtcgtcgtct tgggcggcgc caaatcagag cgttatgaag agatatttca gagaatccga
     9181 tccgcaattg acgccggcgc gtcaggcatc gccgtgggcc ggaacatttg gggttataag
     9241 gagccaggaa agatgaccgc cgcgttatcc gctatcgtgc acggccaggc gtccgtagaa
     9301 agcgctttgc atatgctgca atagtagaat gattcaatag aggtaacgga ggtgttcgga
     9361 tgaccaaacg aagaatggcg atcgtcgtag cgttgatttc gatcgtttcc tgcttttctt
     9421 tttccgtggc cctgatggtc acgggagaaa tcggcggaaa tcctatctat gagatgatgg
     9481 tgagcggcgc cgagtcagcg agcgccgaac tcgggttcga gctgaagact gtcgagggag
     9541 ggtacaactc cgcgaaatgg gaatctaacc tcatcgcttt ggccgccacc gggaagtacg
     9601 atctcatcgt gaccttcaca gaagggatgc cgaagagcgt cgagaaagca gcggatatgt
     9661 tcccgcgtca gaaatttgcc ttaatcgacg gcatcgcggc catcaaaccc aacgtttact
     9721 cactcgggtt taaggacgag gaaatgtgtt atctcgccgg gtatttcgcc ggcttgatca
     9781 cgctctctga tctaccgggc gcgaacccga acctgaccgt cgggctcatc ggcggagaca
     9841 cctatccggc aatgacggac cgtatgaagc cggccttcac gaagggcgcg agggcagtcc
     9901 atcccgatat cgacgttctc ttttcggtgg tgggtagctg gtcggatccc aaaaaaggaa
     9961 aagacctcgc tcaggctcaa tacgcgcagg gcgcggatat tatcctgacg atcgcgggag
    10021 ggtctggcgt aggggcgatt caagaagcgt tgcgcttgaa caaatatatc atcggcgtag
    10081 actccaacac cattccgttg gcgcctggaa cgatcctcgc gtgcgcgctg aaacgagccg
    10141 atctcgcgat gaacgacgtg atgcgtcgcg ccttctccag cgccttgcct tacggcgcta
    10201 cggaacgtca cggtatcgcg gagggagtga tcggtttcac cttcgacgac cccacgtatc
    10261 tcgaaacgat acccgagccg attcgcaaac agatgctgga agtcttcgac gcgttgaaaa
    10321 acggagtcat cgaccccctc tcttaaatgg aaacgcttgc tgttgtttct gctccgcaac
    10381 aaggtaagcc gcgcttctaa aggatgacgg tcaggtgaaa actgtgggcc catatgcgat
    10441 cgagatggaa ggtataagca aagtgttcga aaccaatcga accgtcgcgc tggatcgcgc
    10501 tcgttttcgg gtctctaagg gagcgattca cgcgcttgtt ggcgaaaacg gcgcgggaaa
    10561 atccactctg atgaaaatac tctgtggctt ggagcggaaa aacgccggcg agatccttgt
    10621 cgacggaatc cccaaagaga ttcgaagcgt taaagaggcc tacgcaaacg gtatcggcat
    10681 ggtgaatcag caatttcacc ttatcgaaga ctacactgtc ttggaaaaca tcctccttgg
    10741 agtggagaaa acgaatcgat gggggttaat cgataggcgc gaaagcgcga aagaagttca
    10801 aaaactcatc gaaatgagtc gtctcgaatt gccggcggga aggaaagtgt ccgagctaac
    10861 gatgggacag cgccagttgg tggagatttt gcggatcttg aaccgcgcgg tcgagatcct
    10921 cattttggac gaacccactt ccgtgttgag ccctcgagag atcgactggc tgttttcgat
    10981 catacgcgaa ctgagggcgc gtggaaaaac gatcatcttc atcagccatc gcttggacga
    11041 ggtcttcgaa ctctgcgact cgatcacgat cttgagaaat ggccgggata tcgcggaggg
    11101 gacactctcc gattttgaca agaaacaggt ttcttttttg atgatcggcg aaccactcgg
    11161 cgccctcaac cccaagagga gcccaaaaca atgccccgtt ctcttgagag ctgaaaacct
    11221 ctctgtgagg gatgctgata gaaaaacgga ggtcgtacga tcggtcaata tgatgctccg
    11281 cgggcacgaa atcgccgccc tcgtcggtgt gtccaacaat gggaaacagg agttggtgga
    11341 ggcgttgtcc ggactgaggg agacgtcttc cggcaacata tacctgaagg ataaaaagat
    11401 gacaggctcg acgcccaggg gtttccggaa agccggcatc gcgtttatcc cggaagaccg
    11461 cgtgcgtatg ggcgcctctg tatcctccag tgttctggac aatatcctag cgaccaaata
    11521 cttcgaagcg ccctatacga agcggggttg gatcaatcga aaggaggcgg gaaaaacggc
    11581 ggagtccttg atccgtgagt atcgaattaa gggcgcctct gttcacgcgc ccctggcgac
    11641 cctttcgggg ggaaatatac aaagggtcat cctcgcccga gaaatatcgt cggacccttc
    11701 ggttttgatc ctttgcgaac cgacgtgggg attggaccat cgctcttgtg accacattta
    11761 cgaacgcttg tacgaattag ccgaatcagg aaaagcggtg ttgatcgttt cctcctattt
    11821 ggacgaggcg ttgcgtctcg ccaatcgcat attcgtcatc tataaaggag agatcatcgc
    11881 ggagctcgaa aactcctctt ccctaacaaa aatcgctatc ggcgaattga tgctcggact
    11941 gggccgtcct ctatcccagg gatacgaagg agacggttcg tcgataaagg ccgggagctt
    12001 cgatggtcac acttcgtgat ctttggagaa ccgcgtttat cgtcggcgca gcattgggtt
    12061 ttgggctctg cgtgatcctt ttggcgagcg aaagaccagg cgacgccatc tacgcgtttt
    12121 tcctcggccc cttctccaac gcctatttct tcgggaacct cctggcaagc gccatcccgc
    12181 tgatcctgac gggagcggct gcctgcgttt ctttttccgc gtctgttttt aatctgggcc
    12241 ttgaggggca agtgtatctc ggaagttttt taggtgtctt tttgacttgc cgttttggcg
    12301 aactcccgcc gttggtggtt taccccctcg tcttcgcgat cgtattttgt tgcagcggaa
    12361 cgctggctgc gctttcgggt tacctcaaaa gggcgcgaaa cgtgaacgtg ctgatttcga
    12421 ctctactgat cagctatatc acgatctacg tcgtcgacgc gctcatcgaa acggtgttca
    12481 tagatcctac ggccggactg gccgcgacgc cctacatcca cgacaaattc ctgtttgcgc
    12541 gactgcttcc gccctccgat ctgcactccg gcctgtttct tgcgctcgcc ctcacgataa
    12601 ttttgcgcat cattatgaag aaaagcgcgc tcggtttcga aatcaccttg acgggaaaga
    12661 gcccggtttt cgcgagatat gcgggggttt cggtttctaa ggtcttgacg ctttcgttgt
    12721 tcattagcgg aggattcgcc ggtatcgcgg gaataaccga tatatacggc attcacgggc
    12781 gtatgtttcg gggattctcg agcggttatg gctggaacgg tatagccgtc gccttgatcg
    12841 cgaaaaacca ccccttatgg gtgattccgg ctgcgctgtt ttttgcctac ctcgaaaacg
    12901 gggccaacgt cggcgccttg ttggcggaca tcacccccga aatggcgcga acgatacagg
    12961 cgactatctt tttcaccgtc accgcggacg ctctgttctc gcttattcgg aagaataaag
    13021 gaggtgacgc gtggacacct tcatcaacag tatcgtccct atgatgaccc cggttttgtt
    13081 tgccgctctc ggagggctgt tcacgcatct ttcaggcctc ttgaacatcg gcctggaggg
    13141 cttgatgctc ttgtccgcgt tcttttcgat ctatgtggct cacctcactc acaacctgtt
    13201 cttcgccgtt ctcgccggaa tcgggatttc tatgctcctg gccgtattcg ccggattcct
    13261 ctcgctcaat ctccgcgcca acatcttcgt ggtggggctc ggggtcaacc tcttcgcgaa
    13321 cggcttcacg gtgatgctct cctcgttatt attctctaca aagggaacgg tttttttcca
    13381 gaacttgccg aaactctctt atatagaaat acctctcgtt catcgcattc cctatttggg
    13441 gccgattcta agcggttacg gcgcgttgga ctatctggcg atcggggcgg ccgtattgtg
    13501 cgctattatc ctctataaaa ccccgtatgg ttaccattta cgcgcggtgg gccgggatga
    13561 aaaggcggcg caaaacgccg gaatatccgt ctattggcac agactctatg ccttagcctt
    13621 gagcggcctg ttcgcggggt tgggtggctg cgcgctcgct ctcccgctcc aaacctttgt
    13681 aggcggtatg acgaacggaa ggggatggat cgcactggta gcggtaatat tgggcggggg
    13741 aaagccgagc ggcgtattca tcgcctctct catcttcggc gccgcgtccg ccctatcgaa
    13801 ctggcttcaa gcaagcgcgc caatgtctcc aaaactgatg atgacgttgc cgtttctgat
    13861 cactctgttg tttatgatag ttaagaaaaa taacacggag gaaggtgttt agtatcgata
    13921 gtttttttct tgcgcttaaa gaaagacttc cgcactttgg ccctgcccaa aggaagatcg
    13981 ccgagttcat cattcggaac ccgcgagtca tcgacggcct gacgatccgc gagttggccg
    14041 aaaaaaccgg taccactccc gccgcagtgg tcacattcta taagtccctc tcttacaacg
    14101 gcttccgcgc gatgaaatcg gctctatccg aagagatcga cctggtcgaa tctatgagtt
    14161 tctatctcgt cagaccgtcg aaccaccaca ccgacctgat ctcggccatc cacaataacg
    14221 tgcgttttgc ttcggcgtct ttgtctcaag aatccttgcg tcaagcctgt gatctcctgc
    14281 tgaaaagcaa agcgatcgat atcttagcct ttgggttcga cgctgtcgcc ggccaagacg
    14341 cgttcttgaa gttgaagcaa cttggttttc aatgcaattt tttccagaac gcctgtctac
    14401 aaaaactgtc ggcctcgcac ctcaacaccg aaggatgtgc gctcgcgata tcgagcagct
    14461 tttcttccag ggacattttg gattccattc agttcgctcg gcggcgggga gccaaaacga
    14521 tcgccttcgc ccctcaagaa tcccaagtgc tggaggctgc ggatgtacga ttacccctgc
    14581 aatcgaaatc tgagatattg gccgaagggg ggattctgtc gcgttacgta caacttttcg
    14641 cgatcgactc gctcttcctt aagttgttgg agtcggaccc gctccgtttc gctcagacct
    14701 tcgaacaatt cgaccggatc atccaccaca agtgaaggga caacgcgttg gcagatttgt
    14761 tcggtacgat cgacatcggc tccacgaact gtaaaatagg gatattcgat gaggaggcca
    14821 actttctcta tcgagacgac agcccgatct cgtcccagct tttgagaaga agtgggacga
    14881 tcgacccgtt cgagatttgg gagaccgttt cgggactctt ggggcgattg ccggaacgca
    14941 taaaacgccg attgcgagcg ctgtgcgtca tcgggcaagg gccgaccgtc gttccggtcg
    15001 accggtcggg gcaacccctt tatcgagcga tcacttggtt ggatggacgc ggtaaggagc
    15061 gcatcccggt gttgatcgac caaggggtcg acccgcagct cgccgcgggt ttatccgccc
    15121 tggaatggct gaaagaagag ttgagtgaac aagcggtgtt actacaaccg gcggactacg
    15181 tcgcctatcg gttaaccggc cgtctcgcga atatgtcgtt tccctttgtc ggatacctgc
    15241 cttggagcag acaagcgttg gaagagagag agtggtctca cgcgtttttg gttcccgaga
    15301 tcgtcgagcc gggaagcctg ttttctaatt taacccagga ggctgcggat cgattcaacc
    15361 tatccccgca aacgcttctg gtcgcaggcg ctccagattt tgtagccgcg cttgtcggca
    15421 cggggacgat agaagacgga tctttatgcg atcgaggagg ttcttctcaa ggcgtcacgc
    15481 tctgctcaaa gagcgaggtc tttcctccag ggctgatgac gacgccgttc ttcaagccag
    15541 gcctatggaa gatcagcgcg attatgaaca cgaccgggat cgccttggaa tggttctgcc
    15601 gaaaggtcac tggacagccg ctcgagctca ctctggaaca ggcagtaggg tgcgctacgc
    15661 ccacaaaaat cttgtttctt ccttacttga gtggggagcg gagtcctcat tgggacagca
    15721 aagcgaaagg ggtgttttgg gggctcacct tggatagcga tgcaagacgt atgaccgtgg
    15781 cgatcctgga aagcgtggga tacgcgatag ctgacgcgat cgagcggatg gagaacgccg
    15841 gatgcgtgat caagcggatt cgaacaaccg gaaaacaagc gctcagcccg atctggaccc
    15901 aaatcaagtc agatatcctc ggtaggcccg tagaagttcc ggaaatacgg gagtcggaac
    15961 tatggggagg ggcgctattc tcgatgaaag cagtctcgaa tagggacttg acgttcttga
    16021 gtcacgagaa gacgcgagta ggacaaactt attatcccga ctctaagaaa catctcctct
    16081 acaaagagct gctatcgatg tttcagagcc tatatccagc caacaaaccc ctattcgcgc
    16141 aatgggctcg tatcgatttc gcgcgcggcc ccacaaccca acgggattag gcgaatcgcc
    16201 ctgggaccgc cgaacttcgt gggttatcgc ggggtaccag ctggctgatt tttgaagtcc
    16261 acgaagtctc gcgattcgca ctggaagcac ccagggagtg gcctgggaac accttgggaa
    16321 cgccctggga acgccgaact ttagttcggc atgttttagg ttttttctgg gaacgccaac
    16381 tttttaagtt ggcatggttc ctgttttaca atgaaaaaaa cacaactcat gccacactaa
    16441 agtgtggcgt acccaagggg ccgtatagaa gtcgaaaccg aactttggtt gcacatagaa
    16501 tctgagaacg ccgcactttg cgagttatcg caaggtaacc agcgggctga cttcgggaag
    16561 cccacgaagt ctcactatcc gtcctgaaag cgtcctggga gcggcttggg aacgctagct
    16621 tttaagttgg tatggtttcc tttttacaaa aaaaagcaca actcatgcca cactgaggtg
    16681 tggccttccc caggccctcc cagagaggcg aaccccgccc agcggtccca ggacgtttcg
    16741 agcaattcaa taaacttcgc ttttctcaaa attcctcgga ttttgcgttg tttgttagtt
    16801 taatggtata atgactagcc gttgcgcaga tgggagggat aggatgcgca gaatactgtt
    16861 gctgatctgt ttcgttttct gctggctgtg cggctcggtg ttttcgatga cacaggctga
    16921 gctcgaaggt tacctcgccc taaattatag gaacatcgtc tatgttttcg atcctcagac
    16981 tcttaatatc ctcttttcca acgaacgtgc gtccgagtac tacggataca ccctcgaaca
    17041 gttcacttcg atgaacctta tggacatcag cctactttcc gaggacgatt tgagagatca
    17101 aatcaatgag gctctcgaag taaaacagaa gccgataatc aattcgcaac ggttggctga
    17161 cgggagcatt cggacgatag aagtccacgg ttggcccatc gaagtggatg ggaagacgct
    17221 cttgtttact acggtttttg atgtgacaga gagggagcgc acaaaaaaag agttggaaaa
    17281 atcgctggtg aagcttacaa aagcggagga gatgggcgat gtcggccatt acgagatcta
    17341 tctctccgaa gaaaaaatgg ttctctcaaa aggcgctgaa atgatctacg gactcgtcgg
    17401 cgatctttgg ccaagttcaa cgatctatga attgattctt cccgaagatc gcgaaaaggt
    17461 cgcgaaaagc agagaagagt tggtccgagt gggaaagcgt tacgatgtga ccttctccat
    17521 acggcgcgcc aacgatgggg aactccgata ccttagatcg ctaggcgatt atgataccgt
    17581 gaacaataca atattcggtg tcgtccacga tatcaccgag caaaaaaaag ccgcggcgga
    17641 gctgaaagcc cggaacgacg cgttcttttg gaccctaacg gccggcatcc tcacgataac
    17701 cgcgatcgcc gccctcctgg ctctttcggt tctaaaacag ctaaaggtgg gacgcgagtt
    17761 actagagagt aaaagaaaga tcgatgttca gaacgaagaa ttgcaggcgg caaacgaaga
    17821 gctcg
//
LOCUS       DAOPXN010000029        17682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3242_length_17682_cov_5.651047, whole genome shotgun sequence.
ACCESSION   DAOPXN010000029 DAOPXN010000000
VERSION     DAOPXN010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 17682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17682
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_17682_cov_5.651047"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..785)
                     /locus_tag="PKM49_02805"
     CDS             complement(102..785)
                     /locus_tag="PKM49_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HNY81777.1"
                     /translation="MLPEFKNPSLWTKHRHIDFDLYLFRGTKNGISRYLSHLEWLHAL
                     ERTLRRADFDLWYTKGFHPIPHIGCLRPLPTGVASVAHYFTLRLKRRDGDYPVPDMIR
                     RFNACAPDGLRLRWGSKVFDTFRLDAVAQSWEFSLITEANEQCQPLSLPESFCATRKK
                     DFYVIEYRVNREAWVDYRYVLESLYGTKSPDCFYIPILREVSVSLEKRYPLQWLFSDT
                     EDGRCPKKC"
     gene            complement(769..2499)
                     /locus_tag="PKM49_02810"
     CDS             complement(769..2499)
                     /locus_tag="PKM49_02810"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNY81778.1"
                     /translation="MRFSKLYAPTIKETPSDAEVISHELLVRGGFVRKVAAGVFTFLP
                     LGKRVLKKIEELVRSEMDAAGCQEILMPIIQPADMWKTTGRWEDYGPEMMKMTDRHDR
                     QFALGPTHEEMVTTTVKNELSSYKDLPINLYQIYTKYRDEIRPRFGLLRAREFIMKDA
                     YSFHTSMEDLHQTYQVMYRAYAHILERMGLKFMVVEADTGAIGGSCSHEFNILAANGE
                     SNLYYCPKCGYSASDEKAGYAKAPPYEEESLLALRKVGTPDVKTIEQVSAFLGLPASK
                     IVKSLLFKGREGFIMAMIRGDHEINLSKLRASTGDQTLELAQPDDVSEAFGVPIGFIG
                     PVGIKNSVKIYGDWSVQGLRNVVVGGMETDYHYCDANEGRDYTVSEWYDLRMVKPGDR
                     CPHCSELLLETKGIEMGHIFELGTKYSAALDACYTDEKGQSQPFVMGCYGWGVSRTLA
                     GVVEQMHDEKGIIWPLAIAPYHLIITVVNVNDPLQKETGERLYRHFLERGVEVLLDDR
                     AASAGFKFNDADLIGIPVRLTIGKAVSSGVIELKKRYEKEPVKVRLEDGVDSIYRLYE
                     RMIATYAPGV"
     gene            complement(2914..3714)
                     /locus_tag="PKM49_02815"
     CDS             complement(2914..3714)
                     /locus_tag="PKM49_02815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HNY81779.1"
                     /translation="MTPRENRTPSVRYFDTLPSTNDYLRERWQEEDDWLVVRAGCQTK
                     GHGRLQRPWASPLGGLWFSILFKNAGETPSHYQRLLGVSALRAIEAIDGEAGVFSDLP
                     RTGNGKGFNIKWPNDIVYTNGGPGVLKVAGVLQENLILGEAKAYIVGIGINVNNPIPP
                     ELQGKAVSLRVLFSRDVSIEDLFRAILQEVSAWRSDAPGVLHLWRQRSLLQRGSLVEI
                     YAEAEGVSFSGEVVETPVESIEILCSNGQRRRFFAGEVRLRALTPTGG"
     gene            complement(3711..4436)
                     /locus_tag="PKM49_02820"
     CDS             complement(3711..4436)
                     /locus_tag="PKM49_02820"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HNY81780.1"
                     /translation="MCRMLGVISSKPQRIGLWIPALEIQAKTGMHHPHSDGYGVAFYK
                     DRTLHLRHEVRPVWERSPIVNEDETGTCLVMHARKASKGATTLDNVQPFYARSDDWQL
                     VYCHNGTVRDIDRLDGSEEFLEKGLSDSAVMFHRLIDSLPFYDDLPACMMALILEVEG
                     QCRNWTSLNALFSNGTSLGAVRSCAVEEDYYTLYVGRGADHAVVSTEPFGAYEWALIP
                     NRTLWIFGEGQERFIELDRGAVL"
     gene            complement(4540..5652)
                     /gene="ugpC"
                     /locus_tag="PKM49_02825"
     CDS             complement(4540..5652)
                     /gene="ugpC"
                     /locus_tag="PKM49_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HNY81781.1"
                     /translation="MAQVILENVTKSYDGKEKAVNDANFVVENEEFVVLLGPSGCGKT
                     TTLRMVAGLEEITSGTVKIDGRVVNDVEPKDRDIAMVFQNYALYPHMTVFDNMAFGLK
                     LRKFPKAEIQSRVQEAARILSIEPLLDRKPKQLSGGQRQRVAVGRAIVRNPKVFLFDE
                     PLSNLDAKLRVQMRSELKKLHHNLNATIIYVTHDQIEAMTMADKIVIMRSGLIQQIGD
                     PHQIYNEPANVFVAGFIGTPPMNFVNGIIVQRENALYVREKAGGFALRIPKEIAPKVE
                     EWIEKDVIFGIRPEDLYDKLFAVGEKDVGNIVVADVDVTEPLGNQTILHLKAGETFIV
                     ASVNPQTKAKEGEKIDVVIDMNKMHLFDPNTEKAIF"
     gene            complement(5696..5980)
                     /locus_tag="PKM49_02830"
     CDS             complement(5696..5980)
                     /locus_tag="PKM49_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNY81782.1"
                     /translation="MRRLSDLEFYTLADAKREFSEVIQKAKEQEIVITKNGKPAAMIV
                     DFRRYVKIMDFLSEVYDLFLMDIGLCAPGFPMEKRALDELLLNTKDDRGD"
     gene            complement(6143..8215)
                     /locus_tag="PKM49_02835"
     CDS             complement(6143..8215)
                     /locus_tag="PKM49_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNY81783.1"
                     /translation="MDCRRFFLAVLFFLCAVFSLQARSIVVADVPYYPPYIFLDDRGT
                     PRGITVELWQLWSEKTGMPVEYRFYHTLEEAFEGVQRGESDVINAIFYTEERSLLFDF
                     VYPIDTEPTCIFFPITYRRIDGLRDLEGHQVGVVSGEAVIEHLKTVAPGVDLIVYDDW
                     ESLIKAVSEGRQSVFICDKSIALFYLKRFGKRDLYAFSNPIMPPQELFWCVKAGNKEL
                     SRLLLAGLQSISTEEKAELFWKWKGEKPLSRETLILVIASAVLGLIVIFSILLWNYRL
                     KRIVKKSTRELVEAKDRIEDYNRQLSESNRQLESMNTRLLSANEDLVSGNKQLEEALH
                     EIALISNNLFDVLELSRQLSRETDHEQFFNRLLSLGLKIIPDARKGSISRIEDDQWVF
                     KAIYGYPESLLKLRMPVNIFKPAPADGQIVDIHRYDSERTVPELLLQTTVGIKSSLMV
                     GIYSEERFYGNISWDFFEEVKATNTLIRTGVALGKIASALITIWDHHGELETFQNTII
                     QTLVKAIEIRDIYTRGHSERVAVLARKFAQHIGADENTRQLVYWAGILHDIGKIGIPD
                     LVLNKPLPLTDEEFLTIQTHPVQSARLIEESNLPREIIPIVLSHHERWDGNGYPHGLV
                     GKDIPLEARIISLVDAYDSMTSTRAYRHALSTEHALEEIMRNSGAQFDPTLVRAFLAM
                     FERKEASS"
     gene            complement(8640..10190)
                     /locus_tag="PKM49_02840"
     CDS             complement(8640..10190)
                     /locus_tag="PKM49_02840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY81784.1"
                     /translation="MRKRIGLVWLILLTVSLVMAAVAPTDLVVSIREKGEETGYGVPY
                     EDLEHEFAYMLQYYSQDQSFDPIFDGIFLKYEIANLKVQRELLSYYAMRKGLSIDQLK
                     LEQQVNEVVMNVSNDPETKPEIEAQYGSMENFYQFVLMYYRKDMIRQRAIDDIAPVDE
                     AAMRDYYENNKESIIAANERVSAKHILVQTLEEAEKIKADILSGATTFDEAAANYSLD
                     QSNKYNGGDLSWFTRGDMVEPFEEACFTAPLNALTGPVKTDYGYHLILVTGRQVLSNF
                     EEFKVSEAYETEKQRIQTQGVMAWMPDYMVDKRIEFVFSGTMGSLNTFSKLYPVAAQS
                     GDYAPVFSFLKDYVPVDADGRVFLEVCYQSMLARSEQLPQLMDAAQKAAMFQARLDNL
                     KLLSLEEAYTLAALSRYYSLNPSDTRMAIRFLGKYIDQALELSMDQMIMAYYGNEIRN
                     ELLQAYPYLVRIAEDSAADVRDRVVAFTYMIRINNLTDQKPLNKGILERILEIDPGNT
                     DVLNLVEP"
     gene            complement(10248..10763)
                     /locus_tag="PKM49_02845"
     CDS             complement(10248..10763)
                     /locus_tag="PKM49_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81785.1"
                     /translation="MTPKEIALSGLVGALSVLALWAASVLPGSRLAITFYSAFVLDFL
                     YEYTRLRCGLFVYLCVLCISLLLLPGKMLGVLYGLYFGGYVSVRQFWQKHRWAWLFKG
                     LYLNGVTLLLVAVLLTFFPDLCGRIRDIKVWQFLPLIFVLQIGWVCVDLFLSYVTRVF
                     LRVLADRGAKR"
     gene            complement(10760..11851)
                     /gene="queA"
                     /locus_tag="PKM49_02850"
     CDS             complement(10760..11851)
                     /gene="queA"
                     /locus_tag="PKM49_02850"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNY81786.1"
                     /translation="MNLSDFDYTLPVEKIAQSPVEPRDHSRLMVINRTTRTLEHRRFY
                     HLLDYLLPSDLLVINNTRVIPARLLGAREGGGKAEVFLLKPLRAENEKEALWNALVKP
                     GKRIRKGTVLSFLNHRLIGQCVEHHPDGSRDILFKKNSTDEETDSGEYGSIPLKDLIF
                     RAGSVPLPPYIHRPLSDKERYQTVYSEEEGAVAAPTAGLHFTESLIADIKGQGIEFCT
                     LTLHVGLGTFRPLEDDGGPFAIEGHVMHAETFYVSGEEARKIERARLEKRRIIGVGTT
                     SVRVLESLPYYEKRADGSYAGDTRLFIYPPFPFVYTDAMITNFHLPKSSLLLMVSAFA
                     SRDLMMSAYQEAIESEYRFFSFGDACLIL"
     gene            complement(11848..12894)
                     /locus_tag="PKM49_02855"
     CDS             complement(11848..12894)
                     /locus_tag="PKM49_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81787.1"
                     /translation="MRSIKAALLTVTVLVLATGAVFGAVPRDVSTSLDIYEQVRTIVE
                     ANIMKVDSIGFFNGTNSVNRYDLADALYRVINYIMSTRAIVDASALTRRVEGLESQDE
                     SMRTELALLRQRLDALEVSVSPKELAALERRLVGLKDEVLAEMQGLEQRTKFVSGYSD
                     FLTAVEQELKTLYTRVEEQGSRLAATEANTGRLLSYLKKYETLDTWLPTVDANFAKQE
                     AYNAQMSQAFAELGLQANEILAMKERMEGLQQELSAVGVAAKNALEIPAIVESQNHLG
                     LRVLNLEAANATLLKVQQDLDYLKIENDQLKLENQNVKKQLWYAFGVGIGGAVIGIAA
                     IVYTYVLWGGSQGE"
     gene            complement(12909..14594)
                     /locus_tag="PKM49_02860"
     CDS             complement(12909..14594)
                     /locus_tag="PKM49_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNY81788.1"
                     /translation="MKKCTLIVLGICWMAALLAVPIKIYTQPANVSVYINGTLFGISD
                     RNGVLSEMIFLNAGRYTFRAEKPGYNGHTEVLDIFEATEVTLKLSPSGVLEVKALPVG
                     ALIRVDERFEATGHFLREVSEGRHYVEVSHPGYQSRYFYPEVKQYFTKTLEVALEREG
                     NTRFVTEPAGAQVSVDGIVAGITPFQIFLEPGEHLVNFTKEWHTPQLQMIRVNSLGEN
                     QVFQRLEPFANLTIRSTPVQAKVYKEETFLGETPVTFNQLPLEKMLLTFRAEGYKEVR
                     EEVVLQPGPMTVTGQLALKAYPLTVMSTPAALVYLDGEEKGITPLAFQVEHGSHRLSL
                     KSGDKEWLSVIEVGQPREVSVDLNVETTILFHFVPAGDGFVLHRGQRYPTGQPINTVA
                     GLQTFDLSRSGYPDRRRVYKLSAGKVYEYTIDLEGEALLFLSTDPAQAKVYWLGTLIG
                     ETPLRGLKIRPGTGELKIRWLYGEWAENTSFFDGQTYTLFRQLPGSTTLFVHSFPSGM
                     PLWLNGIPSGLTPQTLYVESGAYTIECEGPDGIRRKQIITLSGEVERTINFVF"
     gene            complement(14679..15254)
                     /locus_tag="PKM49_02865"
     CDS             complement(14679..15254)
                     /locus_tag="PKM49_02865"
                     /inference="COORDINATES: protein motif:HMM:NF027563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4897 domain-containing protein"
                     /protein_id="HNY81789.1"
                     /translation="MNKNWLWLVVIALVGFFIYNIVSSIQSRPQYKQHAYKAVYTFDR
                     SEVTSMYNWVVFSYDKRDDLQKSYDYFQSVSLEEKTNNYAQMLAKLSEQSERKVTLLT
                     YESTARLLAKEMEVEEYSTVRGMAYKSEDFWITGFGVNRLQLQENSSLSFIFPADAEI
                     LSVVPEPDEREGNVLFWETPGQLVFPVVSYR"
     gene            complement(15247..15879)
                     /locus_tag="PKM49_02870"
     CDS             complement(15247..15879)
                     /locus_tag="PKM49_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81790.1"
                     /translation="MSLSRYLFFITLLFLCATGLLLVGVSVLLMDTHEVHTFSSKLYQ
                     FSYDLSHAVIQAHYDLPYSDGPIRLFRESAKSFLEKPVLRLSLFYDPTGVYQDLVRKV
                     LIVVEHFLNTPDGPDPNDLIISYFNVRPSLDDLIGRYRLVSANTLRHIVYATFFFLGL
                     LLVLAILCVFLVFYPLMRDIQNLNHNRRSWIIFKELQSCKLKNRSVKRDE"
     gene            complement(15888..17153)
                     /gene="hisS"
                     /locus_tag="PKM49_02875"
     CDS             complement(15888..17153)
                     /gene="hisS"
                     /locus_tag="PKM49_02875"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNY81791.1"
                     /translation="MINRPKGTNDIYGEEIEYWQSFEKAARKVAGVFGFQEIRTPIFE
                     MAELFIRSVGDETDIVQKEMYTFEDKAHRLLCLRPEGTAPVVRSFIENNMASDGLPKQ
                     VYYMGPMFRYERPQAGRQRQFHQFGAELIGSSSPLADAEMIWMSIFLLEELRLHRYTL
                     QVNMLGCQNDREAYKAALKEHYRDKLDAICPDCRRRYETNALRLLDCKVEGCQTIKEG
                     APILTDFLCPECQNHKRQVSQILAQYNIDATWNPRLVRGLDYYTGIVFEVKYPFKETV
                     FDLLGGGRYDQVIEQLGGSATPSVGFACGIERMVATMREEKAIEEPASLCQVFVLSIG
                     EEAKRESLKITDFLRKRGISVQHDLMGRNIRNQLKYADKQRALYTIIIGEDELDKGVY
                     LVKEMDTGDQIEVESFWVENYILEKLAAL"
     gene            complement(17263..>17682)
                     /locus_tag="PKM49_02880"
     CDS             complement(17263..>17682)
                     /locus_tag="PKM49_02880"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY81792.1"
                     /translation="MARKAQQNAQCEGVEDRVEYVLGNALRMPFSDQSFDFAFSNGSL
                     HEWEQPAAVFKEIHRVLKPGGAFFVSDLKRDAPWYARWLMMATVRPKSMRPGLLSSLH
                     AAYTSAEIRGILADSGWARARVTESAFGFDIEGRKTS"
BASE COUNT         4136 a         4941 c         4423 g         4182 t
ORIGIN      
        1 ttctaacgcg tctaggccgt cctccgcctc cgcgatctca tatccagcct tctttagatt
       61 gaaaacaatg atgttcctga gtaccgtcga gtcatcgatg atcaacactt ttttgggcat
      121 cgtccgtcct ccgtatcgga aaataaccat tgtagcggat accgcttttc taacgacaca
      181 gacacctctc gcagtatcgg gatgtaaaaa cagtcgggag atttcgttcc atataggctc
      241 tctagaacgt aacgatagtc aacccaagcc tctctgttga ccctatattc tatcacataa
      301 aaatcctttt ttcgtgtcgc gcagaaggac tcgggcaaag aaagaggctg acactgttcg
      361 ttagcttcgg taatcagaga gaactcccaa gactgcgcga ccgcgtcaag acgaaatgta
      421 tcgaagacct tcgatcccca tcgcaacctc aacccgtcgg gtgcgcacgc gttgaatcgc
      481 cggatcatgt ccgggacggg gtagtcacca tcccgccgtt tcaagcgtaa ggtgaagtaa
      541 tgggcgacac tcgcgactcc ggtcggtaac ggacgcaaac aaccgatgtg tggaatcggg
      601 tgaaaccctt tcgtgtacca aaggtcgaaa tccgctcgcc tgagcgttcg ctccaacgcg
      661 tgaagccatt ctaaatggga caaataccgg ctgatcccgt tcttggtccc gcggaacaga
      721 tagaggtcaa aatcgatatg ccgatgcttc gtccaaaggg acgggttctt aaactccggg
      781 agcataagtg gcgatcatac gctcatacag gcggtaaata gagtcgactc cgtcttccaa
      841 tcggactttg accggttctt tttcataccg ctttttcagc tcgatcacgc cggacgagac
      901 tgctttccca atggtcagac gcacggggat gccgatgagg tcggcgtcgt tgaacttgaa
      961 tcccgcggag gccgcccgat cgtccaacaa cacttccaca ccccgttcca gaaagtgacg
     1021 atacagtcgc tctccggttt ctttctgaag cggatcgttg acattgacga cggtgatgat
     1081 caggtgatag ggcgcgatcg ccaagggcca gatgataccc ttttcgtcgt gcatttgttc
     1141 gacgaccccg gctagggtgc gggaaacccc ccagccgtag catcccatga cgaacggttg
     1201 actttgcccc ttttcatcgg tataacaagc gtccaacgcg gcggagtatt tggttccgag
     1261 ttcgaagata tggcccatct cgatcccttt cgtctcgagt aaaagctcgg agcaatgcgg
     1321 gcagcgatcg cccggcttca ccatccgcag atcataccat tctgacacgg tgtagtccct
     1381 gccctcgttc gcgtcacagt aatggtaatc cgtctccata ccgcctacca cgacgtttcg
     1441 cagcccttga acgctccagt cgccatagat ttttacggaa ttcttgattc cgacgggtcc
     1501 tataaaaccg atcgggacgc cgaaggcctc ggagacgtca tcgggttggg ctagctccaa
     1561 ggtttgatcc ccggtggagg cccgaagctt ggacagattg atctcatggt ccccgcggat
     1621 catcgccata atgaaccctt cccgaccttt aaacaggagc gatttaacga tcttcgaagc
     1681 cgggagaccg aggaaggcgg agacttgctc gatcgttttg acgtcgggtg tacccacttt
     1741 ccgcaacgcc agaagacttt cttcctcgta agggggggct ttcgcgtacc cggccttctc
     1801 atcggaggcg gaatagccgc actttgggca atagtacagg ttggactctc cgttggccgc
     1861 caagatgttg aactcgtggg agcaagaacc tccgatcgcg ccggtgtccg cttccaccac
     1921 catgaacttc aatcccatcc gttccaagat gtgggcatag gctcgataca tgacctgata
     1981 ggtttgatgc aggtcttcca tcgaggtgtg gaagctgtat gcgtctttca tgatgaactc
     2041 tcgggcccta agcaaaccga accgcgggcg gatttcgtct cgatatttgg tgtagatttg
     2101 atacaaattg atcggcagat ctttatagga agagagctcg ttcttgaccg tggtcgtcac
     2161 catctcttca tgcgtgggtc ccagcgcaaa ctgtcgatcg tgacgatcgg tcatcttcat
     2221 catctcgggc ccgtagtctt cccaccgtcc ggtggttttc cacatatcgg cgggctggat
     2281 gattggcatc aggatctctt gacaccctgc ggcatccatt tccgagcgca cgagctcttc
     2341 gatctttttc aagactcttt tccccaaggg caggaacgta aagacgccgg cggccacctt
     2401 tcgaacgaac cctccgcgaa ccagcagttc gtggctgatc acttccgcgt cggacggcgt
     2461 ctcctttatc gtcggggcgt acaatttcga aaacctcaaa cccttcacct cttttcggga
     2521 gcaacttttc agaattatat cacaaccgcc gagcggatat gtttgaaaat agggaaaaag
     2581 gaagcggccg gaagagaagg cgaatacgcc attccgggag caccgcccga aagcgcctgg
     2641 gaacgccctg ggaacgccaa actttagttt ggtatgtctt ttattttcac ggacactctc
     2701 aggaaaggcc tgccaactta aaagttggcg cgcccagcca acgaagtctc gcaatacacc
     2761 ttctgccttt tacggtgcgc ccagtccaga caaaagcgta tcgcgccttc gcgaaaagaa
     2821 gaagcgctta cgccgcgatg acttctcaaa aaaaccaaaa catgccaact taaaagttgg
     2881 cgtttccagg cgcgcccaga agtgcgatac cccctacccg cccgtagggg tcagggcgcg
     2941 gagtcgaacc tcgccggcga aaaacctccg cctttgcccg ttcgaacaga ggatttcgat
     3001 gctttccacc ggggtctcga cgacctctcc ggaaaatgag acgccctccg cttccgcata
     3061 aatctccacc aaagatcctc gctgtaataa agacctttgg cgccataaat gaagaacgcc
     3121 gggcgcgtcc gaacgccagg cagacacctc ttgaagaatg gcccgaaaca ggtcttctat
     3181 cgacacatcc ctggagaaaa ggacccgaag ggatacggct tttccttgga gttcgggtgg
     3241 gatggggttg ttcacgttga tgccgatacc cacgatatag gccttcgctt cacctagaat
     3301 aagattttct tgcagtacac cggcgacctt cagaacaccg ggacccccgt tcgtgtatac
     3361 gatatcgttc ggccacttaa tgttgaatcc cttaccgttc cctgttctcg gcaggtcgga
     3421 aaaaactccg gcttccccat cgatagcctc tatcgctcgc aaggcggaga ctcccagcaa
     3481 ccgttgataa tgagagggcg tttccccggc attcttgaat aaaatagaaa accaaaggcc
     3541 tcccaatgga gaggcccaag gcctttgcag ccgcccgtgg cctttcgttt gacaaccggc
     3601 ccggacaact agccagtcgt cttcttcttg ccaccgctcg cgaaggtagt cattggtgga
     3661 cggcaacgtg tcgaaatagc gaacggacgg cgttcggttc tcacgcggag tcataagacg
     3721 gcgccgcggt ccaattcgat gaaacgttct tgcccctctc caaatatcca caacgtacgg
     3781 ttcgggatga gtgcccattc gtaagccccg aagggctcgg tggagaccac ggcgtgatcc
     3841 gctcccctcc cgacatagag ggtatagtaa tcctcctcga cggcgcacga tctcacggcg
     3901 cctagagaag tgccgtttga aaagagcgcg ttgagcgagg tccaattacg acactggcct
     3961 tcgacttcca aaatcagggc catcatacaa gcgggcaagt cgtcatagaa aggaagtgaa
     4021 tcgatcagcc gatgaaacat cacggccgaa tccgacagac ctttttctag aaattcttca
     4081 ctcccgtcta gtcggtctat gtcccgaacg gtgccgttat gacagtaaac gagctgccaa
     4141 tcgtccgaac gcgcgtagaa cggttggacg ttgtcgagag tcgtcgcgcc tttgctggct
     4201 tttcgcgcgt gcatcaccaa acaggtgccc gtctcgtcct cattaacgat tgggctccgc
     4261 tcccaaacgg ggcgaacctc gtgccggagg tgtagtgtac ggtctttgta aaaagctacc
     4321 ccgtaaccgt ccgagtgagg gtgatgcatt ccggtcttcg cctggatctc caacgcaggg
     4381 atccataagc cgattcgttg cggttttgaa gagatcacac ctaacattct acacatagga
     4441 cactcctcta aaaacggtgt cgccgtcttt ggcgacaccg catcattctg ggaataaaac
     4501 gcgaggaaca gagcacccga gcagaaaagg cttttaatct taaaatatcg ccttttcggt
     4561 gttgggatca aagaggtgca tcttgttcat atcgatgacg acatcgatct tctctccttc
     4621 tttggccttt gtttgaggat tgacgctcgc cacgatgaag gtctcccccg ccttcaagtg
     4681 aagaatcgtt tggttaccta agggttcggt gacgtcgacg tcggcgacga cgatgttgcc
     4741 gacatccttt tcgcccactg caaagagctt atcgtagagg tcttcgggtc gaataccgaa
     4801 aataacgtct ttttctatcc attcttccac cttaggcgcg atctccttcg gaatgcgaag
     4861 tgcgaaccct cccgcctttt cgcggacgta aagcgcgttc tctctctgta cgattattcc
     4921 gttgacaaag ttcataggag gcgtaccgat gaagccggcg acaaagacgt tggccggttc
     4981 gttatagatc tggtgcggat caccgatctg ctggattaga cccgaccgca taatgacgat
     5041 cttgtccgcc atcgtcatag cctctatttg gtcgtgggtc acataaataa tggtggcgtt
     5101 caagttgtga tgtagctttt tcagctcgct tctcatctga acccgcaatt tagcgtccag
     5161 gttcgaaagg ggctcatcga acaaaaagac ttttgggttc cggacgatcg cccttccgac
     5221 cgccacccgt tgccgttgtc ctcccgaaag ctgcttcggc tttctgtcga gcaggggctc
     5281 gatgctcaga atcctggcgg cttcttgtac acggctttgt atctctgctt tcgggaattt
     5341 gcgcaacttc aagccaaaag ccatgttgtc gaacaccgtc atatgcggat agagcgcgta
     5401 gttctggaaa accatcgcga tatcccggtc cttaggttca acgtcgttga caacccgtcc
     5461 gtcgattttg accgtcccgg aggtgatctc ctccaaaccg gcaaccatcc gtagagtggt
     5521 cgttttaccg cacccggacg gacccaataa aaccacgaac tcctcattct cgactacaaa
     5581 attagcgtcg ttcacagcct tttctttccc gtcgtaactc ttcgtcacat tctccaatat
     5641 gacttgcgcc attcttttac ctcccgtgta tgttcaagat cacatcgatc tctcttcaat
     5701 cccctcggtc gtctttggta ttcaggagca gctcatctaa cgcgcgcttt tccataggaa
     5761 agccgggcgc gcagagcccg atgtccatca gaaacaggtc gtacacctcc gaaaggaagt
     5821 ccataatttt gacatatcgc cggaaatcca cgatcatagc cgcaggtttg ccgttcttcg
     5881 tgatgacgat ctcctgctct ttcgcttttt gtatgacctc agagaactcc cttttcgcgt
     5941 ctgcaagcgt gtagaactcc aaatcggata gccttctcac cgttcacctt tccgtgatga
     6001 ccataattct gatcatatta taccataaca caagaaaaaa agtgaatccg aaaacgggaa
     6061 tattcgaaga cgggaatatt cgaaggaagc gccgaacttc ggttcggtat gtgttttgct
     6121 tttagataga aagcgacgga ctttaagaag aggcctcttt tcgctcgaac atcgcgagga
     6181 aggcgcggac caacgtcggg tcgaactggg cgcccgaatt tcgcatgatc tcttccaagg
     6241 catgctcggt gctcaaagcg tgccgatagg cgcgcgtaga ggtcatcgaa tcgtacgcgt
     6301 ccaccagaga aatgatcctg gcttccagcg ggatgtcttt acccacaagc ccgtgggggt
     6361 acccgttccc atcccatcgc tcgtgatgcg acaagacgat cgggatgatc tcgcggggga
     6421 ggttgctctc ttcgatcaat ctcgcggatt gaaccgggtg cgtctggatt gtcaaaaact
     6481 cttcgtcggt taaaggcagg ggtttgttca aaacgaggtc ggggatgccg atcttcccga
     6541 tatcgtgaag tatgccggcc cagtacacca actgccgggt gttttcgtca gcgccgatgt
     6601 gttgggcgaa tttgcgggct aagacggcca cgcgttcgga atgcccgcgc gtatagatat
     6661 cgcggatttc gatggctttg accaaggttt ggatgatcgt attctgaaag gtctcgagtt
     6721 ccccatgatg atcccagatc gtgatgagcg cggaagcgat ctttccaagc gcgacaccgg
     6781 ttctgatcaa ggtgttggtc gctttcacct cttcaaagaa atcccagctt atgttgccgt
     6841 aaaaccgttc ttccgaatag atgcccacca tcagcgagga tttgataccg accgtcgttt
     6901 gcagcaggag ttctggaact gttctttccg aatcgtatcg atgaatgtcc acgatctgtc
     6961 catccgcagg cgccggtttg aagatgttca cgggcatacg tagttttaac aaagattccg
     7021 gatagccgta gatcgccttg aacacccatt gatcgtcttc gatgcggctg atgcttccct
     7081 ttctcgcgtc cggtatgatc ttcagcccta gggagagcaa ccggttgaag aactgttcgt
     7141 gatcggtttc tcgagagagt tggcggctca attccaggac atcgaagaga ttgttggaga
     7201 tcagggcgat ctcgtgtaag gcctcttcta attgtttgtt gccgctcacc aaatcctcgt
     7261 tggccgataa caatcgcgta ttcatcgatt caagctgtcg attgctttcc gataactgtc
     7321 gattgtagtc ctcgatccgg tctttcgcct cgaccaactc tcgggtggat tttttgacga
     7381 tgcgtttcag ccgatagttc cacaacaaga tggaaaaaat cacgatgaga ccgagaaccg
     7441 cgctagcgat caccaatatt aacgtttctc tagaaagcgg tttttctcct ttccatttcc
     7501 aaaacagctc cgctttttct tcggtcgaaa tcgactgtag acccgcgaga agaaggcgac
     7561 tcaactcttt gttcccagct ttcacgcacc agaagagctc ttgcggaggc ataatcggat
     7621 tcgaaaaggc atacaggtct cttttcccga aacgcttcaa gtagaataag gcaatgctct
     7681 tgtcgcagat aaaaaccgat tggcgtcctt ccgataccgc ttttatcaaa gactcccaat
     7741 cgtcgtacac gatcaggtca acgccggggg cgacggtttt caggtgttcg ataacggctt
     7801 ccccgctgac gaccccgact tggtgccctt ccaaatctcg caggccgtcg atccgccgat
     7861 aggttatcgg aaaaaagata caggtcggtt ccgtatcgat agggtagacg aaatcaaaga
     7921 ggagggagcg ctcttcggta tagaatatcg cgttgatcac atcgctttcc ccacgttgga
     7981 ccccctcgaa agcttcttcc aaggtgtggt agaaacggta ctccacgggc attccggtct
     8041 tttcggacca tagttgccaa agctcgaccg tgatcccccg tggggttccc cgatcgtcta
     8101 aaaagatgta gggtggataa taaggaacgt ccgcgaccac gatcgatctt gcctgtaaag
     8161 aaaaaaccgc gcataaaaaa aacaaaacgg ctagaaaaaa ccgccgacaa tccaacagga
     8221 atcacccttg cgtacctgat attattgtga tgaaaacggt acagacagac tcatcaaccc
     8281 gattatatca catacaaacg cttgtagcaa attcggtgtg ggtataatat cgaggagcaa
     8341 aaaaacggaa agaaaaggat gtaaaaaatg gcccccgaag gagccaacat tggcgctcat
     8401 ttgactcttc aagggagtaa agaagcgtat ttttcgaccc cgcgaagagt acgaagatta
     8461 tcgcggggta accaacggga ccccgcgata ctaacaaagt ccagcagtcc gctctactat
     8521 ttttttcttt aaaactatgt cgaactaaag cggggcgttc cccgaatcgc tcccagtaaa
     8581 gcgctccaaa aaagagccgc tttcgcggct cttttttgat cataacgatc gtcggacgct
     8641 taaggctcca ctaaattcaa aacgtccgta ttccccggat cgatctcgag gattcgttct
     8701 agtatgcctt tattcagagg cttctggtct gtcaggttgt tgatccttat catataggtg
     8761 aaggcgacca cgcggtcccg cacgtcggcg gcagaatctt ccgctattct caccagataa
     8821 gggtacgctt ggagtagctc gtttcgaatc tcgttcccgt aataagccat aatcatctgg
     8881 tccatagaca gctccaaagc ttggtcaatg tactttccca aaaagcggat ggccatccgg
     8941 gtatcggaag ggttgaggct atagtagcgg ctcagggcgg ccaaagtgta cgcttcttcc
     9001 aaagaaagga gcttcaagtt gtcgagccgc gcttgaaaca tcgccgcttt ctgggcggcg
     9061 tccatcaatt gcgggagttg ctcgcttcgg gccagcatac tttgatagca gacttccaag
     9121 aaaaccctgc cgtccgcatc cacgggaaca tagtctttca ggaagctgaa aacgggcgcg
     9181 taatccccgg actgcgccgc gacgggatac agcttcgaaa aggtgttgag actgcccatc
     9241 gtgccggaaa acacgaactc gatccgtttg tccaccatat agtcaggcat ccaggccata
     9301 accccttgcg tttgtatgcg ttgcttctcc gtctcgtaag cctcggagac tttgaactcc
     9361 tcaaagttcg aaagcacctg tcgaccggta acgaggatca aatgataacc atagtccgtt
     9421 ttgaccggcc cggtcagcgc attgagcggc gctgtaaaac aggcttcctc aaatggttcc
     9481 accatatcgc cccgcgtaaa ccagctcaag tccccgccgt tgtatttatt ggattggtca
     9541 agtgagtagt tggcagcggc ttcgtcgaag gtcgtcgccc ctgagaggat atcggctttg
     9601 atcttttccg cttcttccag agtttgcacc aggatatgct tcgcggatac gcgctcgttg
     9661 gccgcgatga tggactcttt attgttttca tagtaatcac gcatcgccgc ttcatccacc
     9721 ggcgctatgt cgtcgatggc gcgctgccgg atcatgtcct ttcgatagta catcaaaacg
     9781 aactgataga agttttccat cgacccgtac tgggcttcta tctcgggttt cgtctccgga
     9841 tcgttggaga cgttcatcac cacttcattc acctgttgtt ccagcttcaa ttgatcgatg
     9901 gataggcctt tccgcatcgc gtagtagctc agtaactcgc gctgaacctt caagttggcg
     9961 atctcatatt tcaaaaaaat gccatcgaag atcgggtcga agctttggtc ttgtgagtaa
    10021 tattgaagca tataggcgaa ctcgtgttcg aggtcctcat atgggacgcc ataccccgtc
    10081 tcttcccctt tttccctgat cgatactacc aagtctgtcg gcgcaacggc cgccatcaca
    10141 aggctcaccg taagcaatat cagccatacc aatcctatcc tttttctcaa aatgaaaacc
    10201 tccttcatat gatgtgccct atattcctat cagaacttcg ctttttctta tcgcttcgca
    10261 cctcggtcgg ctaacacacg caaaaaaacg cgggtcacat aagagagaaa caaatctacg
    10321 caaacccatc cgatttgtaa gacgaaaatc aggggtaaaa attgccaaac cttgatatcc
    10381 cgtattctac cacacaagtc ggggaaaaaa gtgaggagga cggcgaccaa cagcagcgtc
    10441 acgccgttca ggtaaaggcc tttaaacaac caagcccatc ggtgcttttg ccagaattga
    10501 cgcacggaga cgtacccgcc aaagtaaagt ccgtaaagaa cgcctaacat ttttccgggc
    10561 aagaggagca acgagatgca gagcacgcag agatagacga acaggccgca gcgcagccgg
    10621 gtgtattcgt acaaaaaatc caggacgaag gcgctgtaaa acgtgatggc caagcggctc
    10681 ccaggcaaaa cggaagctgc ccaaagcgct aaaaccgaca gcgccccgac cagcccggaa
    10741 agggcgattt ctttcggggt cataggatca ggcaggcgtc tccgaaagag aagaaacggt
    10801 attcgctctc tatcgcttcc tgataggcgg acatcatcag atcccgagac gcgaaggcgc
    10861 tgaccatcag caatagactg gacttgggca gatgaaagtt ggtgatcatc gcatccgtgt
    10921 aaacaaacgg aaagggcgga tagatgaaga gccgcgtgtc tccggcgtag gatccgtcgg
    10981 cccgcttctc gtagtacggc aggctttcca ggacgcgaac cgaggtcgtt ccgacgccga
    11041 tgatccgccg cttctcgagt cgcgcgcgtt cgatcttccg agcctcttcg ccgctgacgt
    11101 aaaaagtttc ggcgtgcata acgtgcccct ctatcgcaaa cggaccccca tcgtcttcta
    11161 agggccggaa ggtccccaac cccacgtgga gggtgagagt gcagaactcg atcccttgac
    11221 ctttgatgtc agcgatcaaa gactcagtga agtgaagacc tgccgtcggg gctgccaccg
    11281 cgccctcttc ttccgaatac accgtctgat agcgctcttt atcgctaagc ggccgatgga
    11341 tataaggggg taaaggaaca cttccggctc gaaaaatcag gtctttcaaa gggatcgatc
    11401 catactctcc cgaatccgtt tcctcatcag tcgagttttt cttgaacagg atatcccgac
    11461 taccgtccgg atggtgctcc acgcactgac cgatcagccg atggttgagg aaagacagga
    11521 ccgttccttt ccgaatccgc ttacccggct tgacgagggc gttccaaaga gcctcctttt
    11581 cgttttctgc acgaaggggt ttgagcaaga agacctccgc ctttccgccc ccctccctcg
    11641 cgccaagcaa tcgcgcgggg ataacgcggg tgttgttgat caccaggagg tcggacggga
    11701 gaaggtaatc gagaagatgg tagaagcgcc gatgctctag ggtgcgtgtg gttctgttga
    11761 tcaccatcaa tcgcgaatga tcccgcggtt ccacagggct ttgcgcgatc ttttcaacgg
    11821 gtagcgtgta gtcaaaatcc gagagattca ctcgccttgg cttcctcccc agagcacgta
    11881 ggtgtagacg atggcggcga ttccgattac ggccccgccg atgccgacgc caaaggcata
    11941 ccacagctgt tttttcacgt tttggttctc cagtttcagt tgatcgttct ctatcttcag
    12001 atagtccagg tcctgctgaa ctttcaagag cgtcgcgttg gccgcttcca ggttcaaaac
    12061 gcgaaggccc aaatggttct gggattccac gatcgctggg atctccaggg cgtttttcgc
    12121 ggcgaccccc actgcagaga gctcttgctg caggccctcc atcctctctt tcatcgcgag
    12181 tatttcgtta gcctgcaaac ccaattcagc aaaagcttga ctcatctgcg cattgtatgc
    12241 ctcttgtttc gcaaagttgg catccacggt cggtaaccag gtgtccaagg tttcgtattt
    12301 tttgagatag ctgagcaggc gaccagtgtt cgcctccgta gcggccaacc gacttccctg
    12361 ctcttcaacg cgggtgtata aggttttaag ctcttgctca acggcggtca agaaatcgct
    12421 ataaccgctc acgaacttcg tgcgctgttc gagcccctgc atctccgcca agacctcgtc
    12481 tttcaacccg accaaacgtc tttccagcgc tgccagctct ttggggctca cagaaacctc
    12541 tagcgcgtcc aatctttggc gtaacaaagc taattcggtt ctcattgact cgtcttggga
    12601 ctccaaaccc tccactcgac gagtcagggc cgaggcgtcg actatcgccc gcgtgctcat
    12661 gatgtagttg atcactcgat aaagggcgtc ggcgaggtca taacggttta cggaattggt
    12721 cccgttgaaa aacccgatac tgtcgacttt cataatgttc gcttccacta tggtacgaac
    12781 ctgttcgtag atatcgaggg acgtcgaaac atctctgggc acggccccga acaccgcccc
    12841 cgtcgctaaa accaagaccg tcaccgtcag aagagccgct tttatagacc tcataaccat
    12901 cctccccatt aaaagacaaa attgatcgtt cgttccactt ccccgctaag cgtgatgatc
    12961 tgctttcgac gtatgccatc tggtccttcg cattcgatcg tataggcgcc ggattccacg
    13021 taaagcgtct gcggtgtcag gcctgacggg attccattca accaaagtgg catcccggac
    13081 gggaaagaat gcacgaagag cgtcgtgctt cctggcagtt gccgaaagag cgtataggtt
    13141 tgcccgtcga aaaaggaggt gttttccgcc cattccccat atagccagcg tatcttcagt
    13201 tcgccggtac ccggacggat ttttaacccg cgtaacgggg tctctccgat gagggtgccg
    13261 agccaataca ctttcgcctg cgcgggatcc gtgctgagga agagcaacgc ctcgccttcc
    13321 aggtcgatcg tgtactcata gaccttacct gcggagagct tgtagacgcg ccgacggtcc
    13381 ggataccccg aacggctcag gtcgaaggtt tgcaaccccg ccaccgtgtt aatcggttgt
    13441 cccgtcggat acctctgtcc ccgatgcaga acgaatccgt cgcctgcggg cacgaaatgg
    13501 aagagaatcg tcgtctcgac gttcaggtcc acgcttacct cccggggttg acccacctcg
    13561 atcactgaca gccactcctt gtctccggat ttcagggaaa ggcgatgaga accgtgctcc
    13621 acctgaaagg caagcggagt gattcctttt tcctctccgt cgaggtagac gagggcggca
    13681 ggggtcgaca taaccgtaag cgggtaggct ttgagggcga gctgacccgt gacggtcatc
    13741 ggtccgggtt gtaggacgac ctcctcgcgc acctctttgt acccttccgc gcgaaaggtg
    13801 aggagcatct tttctagcgg caactggttg aacgtgaccg gcgtctctcc gaggaaggtt
    13861 tcctctttat acactttggc ctgaacggga gtgctccgga tcgtcaggtt cgcgaagggc
    13921 tccagccgtt ggaaaacctg attctctccc aaagaattca cccgtatcat ttgcaattgc
    13981 ggggtgtgcc attctttcgt aaagttcacc agatgttctc cgggctccaa aaagatttga
    14041 aagggcgtga tgcccgccac aatcccgtcc acgctgactt gggcccccgc aggttccgtg
    14101 acaaaacgcg tgttgccctc ccgctccaga gcgacctcca atgtcttcgt aaaatactgt
    14161 ttcacttccg gatagaagta tcgggattgg tatcctggat gcgagacctc tacgtaatgc
    14221 cttccttccg agacctcacg gaggaagtgg ccggtcgctt cgaaacgttc atccacacgt
    14281 atcaaagccc cgacgggcag cgctttcacc tctaagactc cggaaggaga gagcttcaag
    14341 gtcacttccg tagcctcgaa gatgtcgaga acctcggtat ggccgttgta tccgggtttc
    14401 tcggcacgaa aggtgtaacg gcccgcgttc aggaagatca tttccgatag aaccccgttg
    14461 cgatccgata tgccgaaaag cgtgccgttg atgtacaccg aaacattagc gggttgggtg
    14521 taaatcttga tgggcacagc caatagtgcc gccatccagc aaatccccaa tacgatgagg
    14581 gtacacttct tcaaagcttc ctccctatga tcgccagctc ccacgagcga tccgttccaa
    14641 caggttttct atctccaccg tctgcgcgta cgctgtcctt accggtaaga gacgactggg
    14701 aagaccaatt ggcctggtgt ttcccaaaac aacacgttgc cttctctctc gtcaggttca
    14761 gggacgacac ttaaaatctc tgcgtccgcc ggaaagatga aggagagaga agagttctcc
    14821 tgaagctgta aacggttgac tccaaagccg gttatccaaa agtcctcgga tttgtatgcc
    14881 attcctcgga ccgtcgaata ctcttcgact tccatctctt tcgccaaaag gcgggccgtc
    14941 gattcgtagg tcaggagcgt gaccttccgc tccgactgct ccgacagttt cgccagcatc
    15001 tgcgcgtaat tgttcgtttt ctcttccaaa ctgacacttt ggaagtaatc gtagcttttt
    15061 tgcagatcgt cccgcttgtc gtaagaaaag accacccaat tgtacatgct cgtcacttcc
    15121 gaacggtcga aggtgtacac ggccttatac gcgtgttgct tatactgtgg gcgactctgg
    15181 atggaagaaa cgatgttgta gataaaaaaa cccaccagcg caataacgac aagccagagc
    15241 cagtttttat tcatctcttt tcacactccg atttttcaac ttgcacgact gaagttcctt
    15301 aaaaatgatc cacgaacgcc ggttatggtt caagttctgt atatccctca tcagcgggta
    15361 aaacaccaga aagacgcaaa ggattgccaa gaccaagagc aggccaagaa agaaaaaggt
    15421 ggcgtagacg atgtgccgga gcgtgttcgc gctcaccaac cggtacctgc cgatcagatc
    15481 gtccaaagaa ggccgaacgt tgaagtagga gatgatcaga tcgttgggat caggcccatc
    15541 cggcgtgttc aaaaaatgtt ccaccacgat aaggactttc ctgaccaggt cttggtacac
    15601 gcccgtagga tcatagaaca gcgagagccg taagaccggc ttctccaaaa aactcttcgc
    15661 gctctcacgg aacaaccgta tcggcccatc ggaataggga aggtcatagt gcgcttgaat
    15721 cacggcgtga gagaggtcat acgagaattg atacaacttc gaagagaagg tatgcacctc
    15781 gtgcgtgtcc atcagcaaca ccgaaacgcc gaccagcaac aaccctgtcg cgcacagaaa
    15841 gagaagggtg ataaaaaaaa ggtacctaga aagactcaag acggctccta taaggcggcg
    15901 agtttttcca ggatgtagtt ttccacccaa aaactctcta cttcgatctg gtctccagta
    15961 tccatttctt tcaccaaata aacgccttta tcgagctcgt cctctccgat gatgatggtg
    16021 tagagggccc gttgtttgtc cgcgtacttc agctgattcc ggatgttccg ccccatcaaa
    16081 tcgtgttgca cggagatgcc gcgcttcctg agaaagtccg tgatcttgag ggactctcgt
    16141 ttcgcctcct cgcctatcga cagaacgaaa acctgacaca gcgaggccgg ctcttcgatc
    16201 gccttctctt ctcgcatcgt ggcgaccatc cgttcgatcc cacacgcgaa acccactgaa
    16261 ggcgtcgcgc tcccccccag ctgctcgatc acctgatcgt agcgccctcc tcccaagagg
    16321 tcgaaaaccg tctctttgaa cggatacttg acctcgaaga cgatcccggt gtaatagtcc
    16381 agcccgcgga ccaaccgggg gttccaagtc gcatcgatat tgtactgggc gaggatctga
    16441 ctgacttgac gcttatggtt ctgacattcg ggacatagga agtcggtcaa tatcggcgct
    16501 ccttctttga tagtctggca gccctccacc ttacaatcca acaacctcag ggcgttagtt
    16561 tcatatcggc ggcgacagtc cggacaaatc gcgtccaatt tgtcgcgata gtgttctttg
    16621 agggcggctt tataggcttc gcgatcgttt tgacatccga gcatattcac ctggagcgta
    16681 tagcggtgga gtcgcaactc ctccagtagg aagatggaca tccagatcat ctcggcatcc
    16741 gccaacggag aagaggaccc gatcagctcc gcgccaaatt ggtgaaactg tctttgccgt
    16801 cccgcttggg ggcgttcgta ccgaaacatc gggcccatat agtaaacctg cttgggaagc
    16861 ccatcggacg ccatgttgtt ctctatgaag gagcgaacga ccggcgctgt cccctctggg
    16921 cggagacaaa gcaagcgatg cgctttgtct tcaaaggtgt acatctcctt ctgcacgata
    16981 tccgtttcat cgccgacgct acggataaaa agctccgcca tttcgaagat tggggtccta
    17041 atctcttgga atccgaaaac gccggcgacc ttccgcgcag ccttttcaaa agactgccaa
    17101 tactcgattt cttccccgta aatatcgttc gtgcccttcg gacgattgat caatgtgata
    17161 cctccgcctt cgatgatgaa aaaagatcgt cgctattata ccacgattgc gcggcggaga
    17221 accgtgccga tgcggatctg cgaattggcg tctcgtccgg gtttaagagg tctttctccc
    17281 ctcgatgtca aacccgaacg cactttccgt gacgcgcgcc ctcgcccaac ccgaatccgc
    17341 taggatccct ctgatctccg cggaggtata tgcagcgtga agggaggaga ggagccccgg
    17401 tctcatagac ttcggtcgga ccgtcgccat catcagccaa cgggcgtacc aaggcgcgtc
    17461 tcgttttaag tcgctgacga aaaacgcacc tcccggcttc agaacccgat gtatctcttt
    17521 gaaaaccgcc gccggctgct cccattcgtg taatgacccg ttagagaaag cgaagtcgaa
    17581 cgactgatcg gaaaacggca tccttagggc atttcctaag acgtactcga cacggtcttc
    17641 gaccccttca cactgggcgt tctgctgcgc tttacgggcc at
//
LOCUS       DAOPXN010000030        16941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3439_length_16941_cov_5.608551, whole genome shotgun sequence.
ACCESSION   DAOPXN010000030 DAOPXN010000000
VERSION     DAOPXN010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16941
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3439_length_16941_cov_5.608551"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKM49_02885"
     CDS             <1..318
                     /locus_tag="PKM49_02885"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HNY81793.1"
                     /translation="RQAASTVIVEVDGREAYRYSIREDGEYPIRGYQPAVGRSETTHI
                     LHIRNGQVYVEEARCRDKICERMGKIDNPGQLIVCVPYKVLIRLEGDRVVDTGAHPDA
                     VTR"
     gene            332..841
                     /locus_tag="PKM49_02890"
     CDS             332..841
                     /locus_tag="PKM49_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HNY81794.1"
                     /translation="MENNRKVVRIALFVSLALAVYLVENAVPVPVPIPGFKWGFSNFV
                     VLMSLTMLGTRDVLWIVIFKVLLGNFLSGKFLSPVFFISCFGNLSGVLSMIALSKTRC
                     FGYIGISVIGAFANNLAQLAVCGVWLLRSVHIWYFFPYIALMGTVSGVLNALMAKGGE
                     QWFRTYRKN"
     gene            814..1470
                     /locus_tag="PKM49_02895"
     CDS             814..1470
                     /locus_tag="PKM49_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HNY81795.1"
                     /translation="MVPNLSKELIPPEDFVLVLGSSSPRRQELCKRFGLPIVVVNPGG
                     VEETPKQSQVTPQEITTKIALAKLQETERVAANEAAEKIKGKRIVYLAADTMVFLGEE
                     RLGKPLHAQHAEHMLRKLSGRWHDVYTGVGMKIVWNDARVQQMFFCERTRVLFRKLPY
                     QAIQKYVQTGIPLDKAGAYGIQDEGGFFVDQIRGDFYNVMGFPLGRIWELLYNNGVIR
                     "
     gene            1467..2183
                     /gene="radC"
                     /locus_tag="PKM49_02900"
     CDS             1467..2183
                     /gene="radC"
                     /locus_tag="PKM49_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNY81796.1"
                     /translation="MMPREKLEKYGPGELTNAELLAILLRTGTKERGVTVLAEACMEK
                     YGGSLYALERETLEGLSQLKGIGEVKAITIKSALELGVRYYKERLLQRTKITEPEDVF
                     VFCLEMSMLDQEHVRVLSLDSKNRVIGLDDVTKGTVNASLIHPREVFRKAILNSAVAI
                     ILVHNHPSGDPYPSEEDRKITEKITQSGELLSIPCRDHVIIGRGSFFSFTAGRTFLKG
                     DIENGREPRAAEVAEVKRSP"
     gene            2131..3411
                     /locus_tag="PKM49_02905"
     CDS             2131..3411
                     /locus_tag="PKM49_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81797.1"
                     /translation="MEENPELQKLLKLKEALKSDLEKKTGKKIEPKEEKKQKIQLSED
                     LRKRIASDGSVPLNIEEIRTPLDIEKTCAEIQCERLYGYAEKNADQVRDKLLAENLKL
                     LITMLKGRPQEAIRHFERLYVTSKSDFVYFNLLLAELLAGNDVENGVATFLAEQSRSA
                     YPLLMMLFFSLFRYKNFQIVKKTIPLLLKTEPNELHALFFQMLQMDDKEISKYCSILY
                     RKNEYREFQNIAKLLPYFVNKDGTNYQYTLDALNKKDAHHCSRLYYEYITRENALEPA
                     EAMKCPLGLYLAGRTAIQNNDCEKAREYAKRLNAFNDPYGGLLTVALKFGAGKGEEAI
                     RLLHELLLPFERVIVTWLSGRQIPCKGVGTLTNVFKKNTIIFPIAADFEQFQQILTES
                     LAVWDDFEVFLYPYEELRCFFGARLCTLAYKEKK"
     gene            3411..4253
                     /locus_tag="PKM49_02910"
     CDS             3411..4253
                     /locus_tag="PKM49_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81798.1"
                     /translation="MPLFTGKKIFQPMEEWPVYERDWMARISNKGPVLFTGEGLLSQI
                     SKDYSLEVNLSFRRGDFVANETGLLTLRGKLGALIRVRDEIWYVYSQTLLLATMLYRL
                     KETVKRVNPMASVIVRSLPPVKPLDDWYFEAIEDASCICWPSSVTDGVIHQEIWKEFG
                     GIERTLEFYRRSWNPSFKLLLECDRLSDAITAIKSGADGIVLKDLKSEMVGEIVKALR
                     ERFKKIYIEYCGDMTLFNVDQYASLGLDAISTYRADLTKATASSEMVYTLIPAPDTDE
                     PKHS"
     gene            4234..4944
                     /gene="tsaB"
                     /locus_tag="PKM49_02915"
     CDS             4234..4944
                     /gene="tsaB"
                     /locus_tag="PKM49_02915"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNY81799.1"
                     /translation="MSRSIPSDSVEESPAFLLIDASCSTNLVALVVPQRQIRLTRTFT
                     GREFPGENLLSVCEEWMRARGITGKELDFFATSHGPGSLTGLRVAGSFIRTLAQVCQK
                     PVITLNSLWLLERSLGEAEREVVTVIQARQDRYYVRLRSAPDPDEYDLLTAECFFEGC
                     PPEALCCLEERCDLVHRFPDRFAGRDYVLQRLDPDRYIEGVSQAIRNRAGKGYGEIVL
                     HYGGLSVAEEIFYKKMRQ"
     gene            4987..5319
                     /locus_tag="PKM49_02920"
     CDS             4987..5319
                     /locus_tag="PKM49_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNY81800.1"
                     /translation="MKIQNALGDIHIGVSVFKKIVFQSLLESYGLVRMDDVNILNKYL
                     GGDDKGIHIREDDTGITVDVFPVISYGMKIDQVSLNAQENIQYNFQQMLGVVPRAINI
                     HVLGIHIE"
     gene            5331..7121
                     /locus_tag="PKM49_02925"
     CDS             5331..7121
                     /locus_tag="PKM49_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HNY81801.1"
                     /translation="MDKQTDKLTSLALKGAFKKGIEVLLQHKDEVNALNVFPVPDGDT
                     GSNMSACMLEALKEIDKITFESMKNVVQAIRTGTLMGARGNSGVILSQIFSGFCATIT
                     NANVSEISIPLFAEALKKGSEVAKSAVIKPVRGTILTLIDDLAQYSAKISETASDFPV
                     FFQRLTKRSFQVVEKTREMMPKLKQAGVVDAGAKGLAYIIEGFYRYSIGEPINLQVGG
                     EMAGATTQGVFQRETLTYQYCTEFMARLHKGVENGNLRQLKTSLLERGDSLVLVQDSA
                     LLKGHIHTDHPGEIFETVLRYGELMKVKVDNMKEQHESLLGETPTDEPLRTVRSGGHP
                     SSTGYLSPGFTVEVEEPTDIPEVPFVDLETGEVKSMTEEETKENGFVAVSPGEGISRL
                     FKDLNVDQIIPGGQTMNPSTADIAGAIDRVNARHVFVLPNNPNITLAAQSASRMMSGD
                     KKTVYVVESNSVQEGIAAILAFNEIVTPESNFENMNSALKDIVSISVTHAIRDSEIEN
                     NEITEGEYLFFANKELIAHGAVLHDVIMEGLNKVGSDRFLTINIFYGMDIEEPEAQRL
                     REAIAQQYPAAEIEVFEGSQPHYSYFISLE"
     gene            7147..7734
                     /locus_tag="PKM49_02930"
     CDS             7147..7734
                     /locus_tag="PKM49_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNY81802.1"
                     /translation="MSKFEKIVLLTVVALFGVGIVIGLTPHDRAVAEDASRRQQTAKA
                     PVLFPIELNSAGLQELKAIKGIGDVKAKQIITYRQDKGLFRSVDELLEVKGIGPKTLE
                     SIREFIYVDESSLAEAGVYDRAEGTSKSGGRIDINRADLKDLVSLPGIGEVKGKRIID
                     YRETHGRFNEPEELIKVSGIGEKTLQAILPLICCE"
     gene            7908..8414
                     /locus_tag="PKM49_02935"
     CDS             7908..8414
                     /locus_tag="PKM49_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNY81803.1"
                     /translation="MKGKPVLSLIFFVTLGLLMGTVFAGTPATAKLKAPVIAFIDFDK
                     AGQQFTELKTLLQEYQADAEYYNSLLKPLEDEITRLRNLGDSSTTYQLKINEYQLQQQ
                     KFVNQLKMKYDPLAEAKQQRLLELAKEYARINGIDVLLTKVVAIFVADAYDVTDDFIK
                     YANSKATN"
     gene            8389..9123
                     /gene="lptB"
                     /locus_tag="PKM49_02940"
     CDS             8389..9123
                     /gene="lptB"
                     /locus_tag="PKM49_02940"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNY81804.1"
                     /translation="MPTPKLPIETVIRCDNLKKRYGSREVVKGANLIIRSGEIVGLLG
                     PNGAGKTTIFQMILGIIVPSKGKITLDGFEITHLPIHNRAMKGITYLPQEVSVFTGLT
                     VRNNLRLVLENLKYDRKRIEPRISELLFDFGISHLSDQRADSLSGGEKRRLELARMMT
                     LNPKFILLDEPFSGIDPMTVKEIQRLALKLKEKGLGVIVTDHNVEDIRRIVDRLYIIH
                     KGSILAEGLPDAVLSDPAVIANYLGE"
     gene            9167..11503
                     /locus_tag="PKM49_02945"
     CDS             9167..11503
                     /locus_tag="PKM49_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81805.1"
                     /translation="MNNRSVRTRKSSWIRGFALLAALSLLIACFFVACSGGGIQDLNK
                     KNYPPSKPVNLSPENGAVNIGEGPRLEWEPCEDPENDPLTYILLYSTDTINNSKVQTL
                     IATETFALLPDLSLGTWWWRVLVVDAGNNMMRGDRWSFTVGSDNLPKTDSPHFDTDLI
                     VVDIARDGVTLSWPAFYDEENPVSDIVYDLRVDDPLNLSSERKTDLPALVSQEARYLE
                     IATSTEATTIRLTGLSALTRFDGSLVARSASNTQTAVGFFSFTTGNTLPSTPVYLFPE
                     NRAAHLPATPTFRWNLCEDKDGDTLTYDIYIGEDQQTAQRFTRGASEGSYTPQEPLEM
                     GREYLWRIVVKDGKGGNRVGEWWSFTVGNATPISLEPIKPQDGETNVPTRTSLEWSCA
                     DPDGDTLTYTLRFGTAPNKMAVIGKGLKNTVYSFQNPLAYNQKYYWQVLADDGQGVTN
                     RATLTGVVWEFTTQATPNFPPDKPTLQTPGNGATNVSNAPQLTWTCKDDNPEDVLVYT
                     VYTGKSEATLTARGTTTRMSFNLSGLEYQQKYYWKVVADDGRPSSERKTAVSDTWSFT
                     TQATPNFPPDKPTLNTPGNGATNVSNAPQLTWTCKDDNPEDILVYTVYTGKSEATLTS
                     RGTTSQMSFNLSGLEYQQKYYWKVVADDGRPSSERKTAVSDTWSFTTQATPNSPPQAP
                     HTPKPTDGATNVSRSPKLEWQCTDPDNDVLEFAISWGSTNTALTNSATTANRFYSLSD
                     LATGTTYYWRVKADDKKGQAERSQAESPIWNFKTTETK"
     gene            11507..12124
                     /locus_tag="PKM49_02950"
     CDS             11507..12124
                     /locus_tag="PKM49_02950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNY81806.1"
                     /translation="MNGFPMGKALQKFVKMLIDRLFAGLFLVLFSPLFLGIAIGIKRE
                     DRGPVFFRQDRLGKNGRVFKIIKFRSMVPDAIHKGSGLHIQPNDDRITRIGRMLRKTS
                     LDELPQLINILKGEMSFIGPRPPVPTYPKRYEEYEEWVLPRFEALPGMTGLAQVSGRN
                     DIDWYERFRYDIEYVRDWSLLKDIRILFLTFKTVIKGKGIYKAPS"
     gene            13013..13999
                     /locus_tag="PKM49_02955"
     CDS             13013..13999
                     /locus_tag="PKM49_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81807.1"
                     /translation="MRKWVIALLVGLIVASGGMAMFRIGMTAIIDHPALEAAREGIID
                     ELIRRGLVLGTDFVVEFQSAQGNMNTAVAIANYFRSNPFDVVVALSTPSAQTCANAIQ
                     DIPVVFTAVTDPVDAELIPALGKNPGNVVGVSDMTPVRTQFQLIRLVVPKARKVGILV
                     NPGEANSLITVAHAREACAELGLELLEIAGSNTSEMIAALTALVGQVDAVYVGTDNTA
                     ASCVESLHRIAQSNKVPLITGFFTQERANGVISYGFEYYNLGLDTGTIVWDLLHGKPV
                     SELESRLMNANALLLLIDLDTAQDIGLQIPEALMARADYLVVNGVQTRVREK"
     gene            complement(14133..15002)
                     /locus_tag="PKM49_02960"
     CDS             complement(14133..15002)
                     /locus_tag="PKM49_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HNY81808.1"
                     /translation="MTFNAIKRLAVDSGLPLHEAALVADMTESGNNPEETRAQLSEFL
                     RVILEESEKQYGKAQDTLTGLTGTNAKKAHDRGVLYHSRFTHVAIVASLSICESNASM
                     GRVVACPTAGASGVIPGIFYALSKENAFPFAHLLPGFILASAIGNVIAAKATLSGAAG
                     GCQAEIGSAAAMGAGALAYLSGGDAETVDSAAALVLKSLLGLVCDPVGGFVEAPCVKR
                     NAIAVTAAISAAELALSGVTSVIPFDETVDAMRRVGQTLPMELRETGLGGLAATRTAK
                     QLVDRIQTRFQKP"
     gene            complement(14999..15685)
                     /gene="sdaAB"
                     /locus_tag="PKM49_02965"
     CDS             complement(14999..15685)
                     /gene="sdaAB"
                     /locus_tag="PKM49_02965"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HNY81809.1"
                     /translation="MSLVNVGGPVMIGPSSSHTLGALRIGRFGNYFLGKTPDKAEFFL
                     HQSFGQTYMGHGTDRALLAGVMGFGDADRAVKDALGIALSRGLVYSFHQEDLGEVHPN
                     TVKMLLYDGADSQEIWASSIGAGMIRIIKINGVECDLSGAYPTLILVNKDIPGALNMI
                     LEKIDANVANLYLRRINKLLAKALTIVELDSDLSLRSLLEIKSLPVILEAYYVKSLSD
                     VAQDRVGGLL"
     gene            16535..>16941
                     /locus_tag="PKM49_02970"
     CDS             16535..>16941
                     /locus_tag="PKM49_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNY81810.1"
                     /translation="MRIRVHLMFCLFLFIALSLFGIVRIGITAIIDHPALDAIRLGII
                     DGLIQDGLVLAQDFDFEFQSAQGNMSTALAIANHFNTDAFDIIVAISTPSAQACANVI
                     KSKPVVFSAATDPVAAGLITVLGKNPGNVVGVS"
BASE COUNT         4288 a         4294 c         4620 g         3739 t
ORIGIN      
        1 cgtcaggccg cttcgaccgt gatcgtcgaa gtagacggcc gagaggccta tcggtattcg
       61 attcgggagg atggagagta cccgatacgg ggttaccagc ctgcggttgg aaggagcgaa
      121 acgactcaca tattgcacat tcgaaacggc caagtttacg tagaggaggc gcgctgcagg
      181 gataaaatct gcgagaggat gggaaagatc gacaacccgg ggcagctcat cgtctgtgtc
      241 ccgtataaag tgctgatccg tttggaaggc gatcgagtcg tggataccgg cgcccacccg
      301 gatgcggtga cccgataagg gatggcagga cgtggagaac aatcggaagg tcgttcgtat
      361 cgcgctgttc gtctcgcttg ccctcgcggt ctatctcgtc gagaacgccg tcccggttcc
      421 ggtccccatt ccggggttca aatggggttt ttcgaatttc gtcgtattga tgtcgctgac
      481 gatgttgggt acgcgggacg ttttatggat cgtcattttt aaggtgttgc tagggaattt
      541 cttatctggg aaatttctgt ctccggtttt ttttatcagc tgttttggaa acctcagtgg
      601 cgtcttgtct atgatcgcgt tatcgaaaac gcgctgtttc ggttacatcg gcatcagcgt
      661 cataggcgcc tttgcgaata acctcgcgca gctagcggta tgcggggtgt ggctgctgcg
      721 ctccgttcat atatggtatt tctttccgta catcgcttta atgggtacgg tttctggcgt
      781 tttgaacgct ctaatggcca agggagggga acaatggttc cgaacctatc gaaagaattg
      841 ataccaccgg aggatttcgt gttggtgttg ggctcgagta gcccaagacg tcaagagctc
      901 tgcaaacgtt tcggtttgcc tatcgtcgtc gtaaacccgg gaggtgttga agaaacgccc
      961 aaacagtcgc aggtcacacc tcaagagatc actaccaaaa tcgcgctcgc gaagctccag
     1021 gagaccgagc gggtagcggc gaacgaagcg gcggagaaga tcaagggaaa gcgaatcgta
     1081 tacctagccg ccgatacgat ggttttttta ggcgaagaac ggctggggaa acctctacac
     1141 gcccaacacg ccgaacatat gctccgcaag ctctcaggcc ggtggcacga cgtgtacacc
     1201 ggagtcggga tgaagatcgt gtggaacgac gcgagagtgc aacagatgtt tttttgcgag
     1261 cggacacgcg tattgtttcg taagcttccc tatcaagcga tacaaaagta tgtccaaacg
     1321 ggtatccccc tcgataaggc gggcgcttac ggtatacagg atgaaggtgg gttctttgtc
     1381 gaccaaatca gaggcgattt ctacaatgtg atgggtttcc ccttaggaag aatatgggaa
     1441 ttattatata ataacggtgt cattcgatga tgcctcgaga aaagctggaa aaatatggac
     1501 ccggcgaatt gaccaacgcg gagctgttgg ccatactgct gaggacggga acgaaggaga
     1561 gaggcgtcac agttctcgcc gaagcgtgta tggaaaaata cggaggctcc ctttacgcgc
     1621 tcgagcgtga aacgctcgaa gggctgagcc agttgaaagg cataggagaa gtcaaggcga
     1681 ttacgatcaa gtccgcgttg gaattagggg ttcgttatta caaagagcgc ctgttgcaaa
     1741 gaacgaagat caccgaacct gaagacgtct tcgtcttttg tctggagatg tctatgttgg
     1801 atcaggagca cgtccgggtc ctgtctttgg attcaaaaaa ccgtgtcatc ggtttagacg
     1861 acgtgacgaa aggaacggta aacgcctcgt tgatccatcc gagagaggtt tttcggaagg
     1921 ccatcttgaa ttcggcggtg gcgatcattc tggtgcataa ccacccgtct ggggatccgt
     1981 atccgagcga agaagaccgg aagataacgg aaaaaatcac gcagagcggc gagctcctct
     2041 cgattccctg tcgggaccac gtgatcatcg ggagaggcag ttttttcagc ttcaccgccg
     2101 gaaggacttt tttgaaggga gacatcgaga atggaagaga accccgagct gcagaagttg
     2161 ctgaagttaa aagaagccct taagtcggat ttagagaaaa aaaccggaaa aaaaatagag
     2221 ccgaaggaag agaaaaaaca aaagatacaa ctctccgaag acctgagaaa acgaatcgcc
     2281 agcgatggaa gcgttccgtt gaacatagaa gagatccgta cgcccttaga tatagaaaaa
     2341 acttgcgcgg agattcaatg tgagagactg tacggctatg cggaaaagaa cgcagatcaa
     2401 gttcgtgaca agctccttgc cgaaaacctc aaactcttga taacgatgct gaaaggccgt
     2461 cctcaagagg cgattaggca cttcgaaagg ctgtatgtga cctcaaagag cgatttcgta
     2521 tatttcaacc tcttactggc cgaactactc gcgggaaacg acgtggaaaa cggggtcgcc
     2581 acctttttag ccgaacaatc acggtcggcc tatcccttgt tgatgatgct attcttctcg
     2641 cttttccgtt acaagaactt ccaaatcgtg aaaaagacga tccctctgtt gctcaaaacc
     2701 gaaccaaacg agctccacgc actatttttc cagatgcttc agatggacga taaagaaatt
     2761 tcaaagtatt gctcgattct ctaccgcaag aacgaataca gagaatttca aaatatcgcc
     2821 aaactgcttc cctatttcgt gaacaaagac ggcacaaact atcaatatac actcgacgcc
     2881 ctgaataaaa aagacgccca tcactgttcc cgtctctatt acgagtacat cacccgggaa
     2941 aatgcgctgg aaccggcgga ggcgatgaaa tgccccctgg gtctctacct cgccggacga
     3001 accgcaattc aaaacaacga ttgcgaaaag gcgcgagagt acgccaaacg gttgaatgca
     3061 tttaacgatc cctatggcgg gcttctgacc gtcgccttga aatttggcgc cgggaaaggg
     3121 gaagaggcta ttcgcctttt gcacgaactc cttcttccat tcgagagagt catcgtgacc
     3181 tggctcagcg ggaggcagat cccgtgcaaa ggcgtgggaa ccctcacaaa tgtcttcaaa
     3241 aagaacacaa tcatctttcc gattgcggcc gatttcgagc aattccagca gatcctgacc
     3301 gaaagcctgg cggtgtggga cgattttgag gtattccttt atccgtatga agaactgcga
     3361 tgtttctttg gcgcccggtt gtgcacccta gcctacaagg agaagaagtg atgccgctct
     3421 tcaccggaaa gaagatattt caaccaatgg aagaatggcc cgtttatgag cgggactgga
     3481 tggccaggat ctccaacaaa ggccccgtcc tgtttacagg agaagggctt ctatctcaga
     3541 tatccaaaga ctattcgttg gaagtgaacc tctctttccg tcgcggggac ttcgtcgcca
     3601 atgaaacggg actattgacg ttgaggggga aactcggggc cctgattcgc gtcagagacg
     3661 aaatatggta cgtgtatagc cagacgctcc tgctcgcgac gatgctgtat cgactgaaag
     3721 aaaccgtcaa acgcgtcaat ccaatggctt ccgtcatcgt tcgctccctc cctccggtga
     3781 aacctttgga tgattggtac ttcgaagcga tagaggacgc gagctgtatc tgttggccct
     3841 cgagcgtgac cgatggggtc atccatcaag agatatggaa agagttcggg ggaatcgaga
     3901 gaacgctcga gttttaccgt cgctcttgga atcccagttt caagctgtta ctcgagtgcg
     3961 accggctatc tgacgccatc acggcgataa aaagcggagc ggacgggatt gttctcaaag
     4021 atttgaagag cgagatggtg ggagagatcg ttaaggcttt acgcgaacga ttcaaaaaga
     4081 tatacatcga atactgcggg gatatgaccc tttttaacgt ggaccaatac gcttcactgg
     4141 gattggacgc catttcgacc tatcgagccg atcttacgaa agcgaccgct tcgtcggaga
     4201 tggtctacac gctgataccc gctccggata cggatgagcc gaagcattcc tagcgactcg
     4261 gttgaggaga gcccggcctt cctactgatc gacgccagtt gttcgaccaa cctcgtcgcc
     4321 ttagtcgttc cgcagcgcca gataagattg acacggacct tcactggtcg agagttcccg
     4381 ggagaaaacc tgctgtccgt ttgcgaagag tggatgagag cgcgagggat aacaggaaaa
     4441 gagttggatt tcttcgctac gtcgcacggc ccggggtctt tgacggggct gcgtgtcgcc
     4501 ggttctttca tccgcacgtt ggcccaggtt tgtcagaagc cggtgatcac cctcaacagc
     4561 ttgtggctgc tcgaacggtc tttgggagaa gctgaacgcg aggtcgtcac cgttatacag
     4621 gccaggcagg accggtacta tgttcggctc cgaagcgctc cggacccaga tgaatatgat
     4681 ctgctgaccg ccgagtgttt tttcgaaggc tgtcctccgg aggcgctctg ttgtttggaa
     4741 gaaagatgcg acctcgtcca ccgcttccca gatcgattcg ccggacgaga ctacgtttta
     4801 cagcggttag accccgatcg gtatatcgag ggtgtatcgc aggcgatacg gaaccgagca
     4861 ggaaaggggt acggagagat cgttttgcat tacggcggcc tatcggttgc cgaagagata
     4921 ttttacaaga aaatgcgcca atgatatcgc gcagggtttt tatgttcact ttaaggaggt
     4981 tgagaaatga aaatacagaa cgctttggga gatatccaca taggagtcag cgtatttaag
     5041 aaaatcgtat tccaatcgct cttggaaagt tacggattgg ttcgaatgga cgacgtcaac
     5101 attctgaata aatacctggg cggagatgac aaaggaatac atatacgaga agacgacacc
     5161 ggaatcaccg tagatgtctt ccccgtgatt tcttatggaa tgaagatcga tcaagtctcg
     5221 ctcaatgctc aagagaatat ccaatacaac ttccagcaga tgcttggggt ggttccccgg
     5281 gccatcaaca tccacgtgtt gggcattcat atcgagtagg ggcgtttctt atggacaaac
     5341 aaaccgacaa gttgacctcg ctcgctctga aaggggcttt caaaaagggc atcgaggttt
     5401 tgctgcaaca caaagatgaa gtcaacgcgt tgaacgtctt tcctgttccg gacggcgata
     5461 cgggttcgaa catgagcgcc tgtatgctcg aggccctgaa agaaatcgac aagatcacct
     5521 tcgagtcgat gaagaacgtc gtccaagcga tacgcacggg cacgctgatg ggcgcccgcg
     5581 gcaattccgg ggtgatctta tctcaaatct tcagtgggtt ctgcgcgacc atcacaaacg
     5641 ccaacgtctc cgaaatcagc attcctttat tcgctgaagc ccttaaaaag ggaagcgagg
     5701 tggcgaagag cgccgtcata aagcccgtga gagggacaat tttgacgttg atcgacgact
     5761 tggcccaata ctccgcgaag atttcggaaa cggcctccga ctttcctgtc ttttttcaac
     5821 gcttgacgaa acgttctttc caggtggtgg agaaaacgag agaaatgatg cccaagctta
     5881 agcaagccgg cgtagtggat gccggcgcca aagggctagc gtacatcata gaagggttct
     5941 accggtactc tatcggagag ccgatcaacc tacaagtagg tggagagatg gccggcgcga
     6001 cgacgcaagg ggtttttcaa cgggagacat taacctatca gtattgcacc gaatttatgg
     6061 cgcggttgca taaaggtgta gaaaacggca acttacgcca gctaaagacc agtcttttag
     6121 agcgtggaga ctccctggtt ctcgttcagg atagcgcgct gttgaaaggc catatccaca
     6181 ccgatcatcc cggagagata ttcgaaacgg tcttacggta cggagaactg atgaaggtaa
     6241 aagtggacaa tatgaaggag caacacgagt cactactggg agaaacgcct acggacgaac
     6301 cgctaagaac cgtacggagc ggtgggcacc caagttccac aggttacctg agccccggtt
     6361 ttaccgttga ggtagaagaa ccgacggata tcccggaggt gccgttcgtc gatctcgaaa
     6421 ctggagaagt gaagtcgatg actgaggaag agacgaagga gaacggcttc gtcgcggttt
     6481 cgccaggaga aggaatcagc cgattgttca aagatttgaa cgtggaccag atcattccgg
     6541 gggggcaaac gatgaacccc tccaccgccg atatcgcggg ggcgatcgat cgcgtgaacg
     6601 cccgtcacgt cttcgtccta ccaaacaacc cgaacatcac gttagcggcc cagagcgcgt
     6661 ctagaatgat gagcggagat aaaaaaaccg tctatgtcgt tgaaagcaat tcggttcaag
     6721 agggcatcgc cgcgatcttg gccttcaacg agatcgtgac gccggagtcg aatttcgaga
     6781 atatgaatag cgcgctcaaa gacatcgtct ctatttctgt cacacacgcg attcgagatt
     6841 cggagataga gaacaacgag atcacggaag gcgagtatct gtttttcgcc aataaagagc
     6901 taattgcaca cggcgctgtg cttcacgatg tgatcatgga gggattgaac aaggtgggtt
     6961 ccgatcgatt cctcacgatc aatatctttt acgggatgga tatagaagag ccggaagcgc
     7021 aaagactgag ggaagccatc gcccaacagt atccggccgc agagatcgag gtgttcgaag
     7081 gaagccaacc gcactattcc tacttcatat ctctggagta gggacccttt ggcgcaaggg
     7141 ggcgcgatgt ccaaattcga aaagatcgtt ttgcttacgg tagtcgctct cttcggggta
     7201 ggcatagtga tcgggctgac gccccacgac cgggcggtgg cggaagacgc gagccgccga
     7261 cagcagaccg cgaaagcgcc ggttctattt ccgatcgagc tgaatagcgc gggccttcaa
     7321 gagctgaagg cgatcaaggg catcggcgac gtgaaggcga aacaaatcat aacctatcgg
     7381 caagacaagg gtctttttcg ttcggtcgac gagctattgg aagtcaaagg cattgggcct
     7441 aaaacgttgg aatcgattcg agagttcatc tacgtcgatg agtcttcctt agcagaagcc
     7501 ggcgtatacg atcgcgcgga ggggacttcc aaatccggag ggaggatcga tatcaatcgc
     7561 gccgacctga aagacttggt ctcgttaccc gggatagggg aagtcaaagg gaagcggatt
     7621 atcgactacc gggaaacaca cgggagattc aacgaacccg aagagttgat taaagtctcg
     7681 ggcatcggcg agaaaacctt acaggcgatt ttacccttga tctgttgcga gtgaggggta
     7741 gcgcatcgcg cctgggttcc ggtgatagat ggaacgattc agcggctttc tttgaagtcc
     7801 taccgctatc aaggtaaaat atctattaaa gagaaacacg ggaagaacca taaaaccacc
     7861 gaggacaaag aggacatttt ttgtatttta cacaggagga tggaaacatg aagggaaagc
     7921 cagtcttatc gcttatcttt tttgtgacac tggggttgtt gatggggacg gtctttgcgg
     7981 gaacgccggc aacagcgaaa ctgaaagcgc cggtaatcgc gtttatcgat ttcgataaag
     8041 ccggtcagca gttcaccgaa ttgaagacgc ttttgcaaga gtatcaagcg gacgccgaat
     8101 actacaatag cctacttaaa ccccttgaag acgagatcac ccgcttgcgt aacctcgggg
     8161 actcgagcac gacctaccag ctgaaaatca acgagtacca attacagcaa cagaagttcg
     8221 tcaaccagct gaagatgaag tacgacccgt tagcggaagc taaacaacag cgtttgttgg
     8281 aattggccaa agaatacgcc cggataaacg ggatcgatgt gttgctcacc aaagtagtcg
     8341 cgatatttgt ggcagacgcc tacgatgtca cggacgattt catcaaatat gccaactcca
     8401 aagctaccaa ttgagacggt cattcggtgt gataatctaa agaaacgata tggcagtaga
     8461 gaggttgtaa aaggagccaa tttaatcatc cgttcgggag aaatcgtcgg actgttagga
     8521 ccgaatggag cgggaaaaac gacgatattt cagatgatcc tcggcatcat cgtcccctcg
     8581 aaagggaaga tcacgctaga cgggttcgag atcacccatc tgccgatcca taatcgggcg
     8641 atgaaaggga tcacctatct acctcaagaa gtttcggtct ttaccggtct aacggtaaga
     8701 aacaatttac ggttggtgct cgaaaacctg aaatacgatc ggaaaaggat cgagccgagg
     8761 atatcggagt tgctcttcga tttcgggatt tcccacctgt cggaccaacg ggccgatagt
     8821 ctatccggag gggaaaagcg gcgtttggag ctggcgagga tgatgactct caaccccaaa
     8881 ttcatccttc tggacgagcc cttcagcggg atcgacccta tgacggtcaa agagatacag
     8941 cggctcgcct tgaaactgaa agagaaggga ttgggggtca tcgtgacgga ccataacgtc
     9001 gaggatatac gaagaatcgt cgatcgcctt tacatcattc ataaaggctc tattttggcg
     9061 gaggggctgc cggatgccgt tttgtcggat ccggccgtga tcgccaacta tcttggcgag
     9121 tagacttttt taggcgcgtg aggggtattt ttggggaggg atgtctatga acaaccgaag
     9181 tgtgagaaca cgtaaatcct cttggattcg aggatttgcc ttattggccg ctctttccct
     9241 tctgatcgcc tgtttcttcg tggcctgctc ggggggtgga atccaagact taaacaaaaa
     9301 aaactacccg ccctccaaac ccgtgaatct gtcgcctgag aatggggctg tcaatatagg
     9361 tgaagggccg aggttggagt gggaaccgtg cgaggacccg gaaaacgatc cactgaccta
     9421 tatcctttta tactccacgg atacgatcaa caacagcaaa gtgcaaacgc tcatcgcgac
     9481 ggaaaccttc gccttgcttc ccgacctatc cctaggcacc tggtggtggc gcgttctggt
     9541 tgtggatgcg gggaacaata tgatgcgcgg cgaccggtgg agtttcactg tgggaagcga
     9601 caacctcccc aagaccgatt ccccccattt cgataccgac ctcatcgtcg ttgacatcgc
     9661 ccgcgatgga gtgacgctct cttggcctgc cttctacgat gaggaaaacc cggtgagcga
     9721 tatcgtctac gacctcaggg tcgatgaccc gttgaacttg agctcggaaa ggaaaaccga
     9781 cttgcccgcc ttagtatcac aggaggctcg atacctggag atcgcgacct ctacggaggc
     9841 gactacgata cggttgactg gcctttccgc gttaacccgt ttcgatgggt cgctggtggc
     9901 gcgaagcgcg agcaacacac aaaccgcggt ggggttcttt tctttcacaa ccggaaacac
     9961 tttgccttcc acccccgtct acctctttcc ggagaatcgg gcggcgcatc tgcccgcgac
    10021 ccctaccttt cgctggaatc tatgcgaaga caaggacggc gataccctca cctacgatat
    10081 ctacataggc gaagaccagc agacggcgca acgcttcaca cgcggcgcct ccgaagggag
    10141 ctataccccg caagaaccct tggaaatggg cagagaatac ctctggcgga tcgtggttaa
    10201 ggacgggaag ggcggaaacc gcgtcggaga atggtggtcc ttcacagtgg gtaacgccac
    10261 accgatttcg ttagaaccga tcaaacctca ggacggtgag acgaacgtgc ccacgaggac
    10321 gagcctggaa tggtcctgcg cggacccgga tggcgacact ctgacctaca cccttcgctt
    10381 cggcaccgcc cccaacaaga tggcagtgat cggtaaaggg ttaaaaaaca ccgtttactc
    10441 attccaaaac ccgctcgctt acaatcaaaa atactattgg caagtgctgg ccgacgatgg
    10501 gcagggagtg acaaaccggg cgaccttgac cggcgtggtg tgggagttca ccacacaggc
    10561 caccccgaac ttcccgccgg acaaaccgac cttgcagacc ccggggaacg gcgcgacgaa
    10621 cgtctcgaac gcgccacagc tgacttggac ttgcaaagac gacaacccgg aggacgttct
    10681 ggtctacacg gtctatacag gtaagagcga agcgacgttg accgcccgtg gaacgaccac
    10741 acggatgtct tttaatttgt ccgggctgga ataccaacaa aagtactatt ggaaagtggt
    10801 cgccgatgac ggtcgaccat cctccgagag aaaaaccgcc gtctctgaca cctggtcttt
    10861 caccacgcag gccaccccga acttcccgcc ggacaaaccg accttgaata ccccggggaa
    10921 cggcgcgacg aacgtctcga acgcgccaca gctgacttgg acctgcaaag acgacaaccc
    10981 cgaagacatc ctggtctaca cggtctacac tggaaagagc gaagcgacgt taacttcccg
    11041 cgggacgacc tctcagatgt cttttaattt gtcagggttg gagtaccagc agaagtacta
    11101 ttggaaagtg gtcgccgatg acggtcgacc atcctccgag agaaaaaccg ccgtctccga
    11161 cacctggtct ttcaccacgc aggccacgcc gaactcgccg ccccaagcgc cacacacccc
    11221 gaaaccgacg gacggagcga ccaacgtgtc gcggtccccg aaattggaat ggcagtgcac
    11281 ggacccggac aacgatgtct tagaattcgc gatatcctgg gggtcgacga atacggcgct
    11341 cacaaactcg gcgaccacgg ccaacaggtt ctattcgctg tccgacctcg cgacggggac
    11401 gacctactat tggcgcgtga aagcggacga caagaaaggg caagccgaac ggtcccaggc
    11461 cgaaagtccg atatggaact ttaaaacgac agaaacgaag taaggtgtga acggttttcc
    11521 gatgggcaaa gccttgcaaa aattcgtgaa aatgttgata gaccggttgt tcgccggtct
    11581 ttttttggtg ttgttctcgc cccttttctt aggtatcgca ataggcatca aaagggagga
    11641 ccgcggaccg gtcttcttta gacaggatcg gctgggaaag aacgggcgcg tcttcaaaat
    11701 cataaaattc agatcgatgg ttccggatgc gatccacaaa gggtcgggct tgcatataca
    11761 accgaacgac gatcgtatca cccgcatcgg tagaatgcta aggaagacca gcttggacga
    11821 gttaccgcag ctcataaaca tcctgaaggg agagatgagc tttatcggcc ccagaccgcc
    11881 agtaccgacc tatcccaagc ggtatgagga gtacgaggag tgggtattgc cacgcttcga
    11941 agcgctgccc ggtatgaccg gcttagcgca ggtgagcggc cggaacgaca tcgattggta
    12001 tgaacgcttc cgatacgaca tcgagtacgt tcgagattgg tccttgctga aggatattcg
    12061 cattctcttt ctaaccttca aaacggtcat caaagggaaa ggtatctaca aagccccatc
    12121 ctgagcgact ctgaaagcga ttttcggaga ctacgtcgac accacgagac cgaacgcttg
    12181 acgtgtgtgt caagttatga ggtctgatca agaaacaagg ccagaatcga ccgtacaatt
    12241 ttagtaatca tagtggcgca ataaagacaa aaggccgatc gcggggtccc ggcagccatc
    12301 gcggcgtaag cgccccttct cttcgcgacg gcgcgatacg cttgggaacg cctgggaacg
    12361 ccagctttta agttggtatg tctaaaaata aaacaggggt tcgcgcagag agcacaggtt
    12421 atcgcggggt aaccagcggg ctgacttcgg aagcccacga actctcgcga tcctccattt
    12481 gtcttttttt gttgcaagtt aaacatgccg aactaaagtt cggcgttccc aaaaacaggg
    12541 gttcgccaga gagcacagaa aaaaagattc ttacgaacgt ctgggaatgc cttggaaacg
    12601 tcgcgcttta gtgcggcagt ttttctggga acgccaactt ttaagttggc ttgtcttaaa
    12661 agaaaaagat aggagagaac gcatagctcg acttcgttag tatcgcgggg gcccgttttt
    12721 tccctcctcc tcttgccttt tttatgtacc tcgatattat actcacacag acttgacttt
    12781 gtcgaataaa atgtggtata actcgggtaa gtttttctcg gtaaggatgg gatcttatgt
    12841 ctaattgttt gtctttaacc gtcgtcaata atagccaaca gttcgttttc gcttttcgaa
    12901 caaagccctc ttgtggtttt gtttgaactt acgcataaaa aacacaaaac tacaaaaaga
    12961 gggcactgaa agaaagtgcc ctctttttat ttttggaaga ggaggcaaca aaatgagaaa
    13021 atgggtcatc gcgctgttgg taggtttgat tgtggcaagt gggggaatgg cgatgttccg
    13081 aatcggtatg acggcgatca tcgatcaccc ggctttggaa gccgcgcgag aggggatcat
    13141 tgacgaattg atccgcagag gcctcgtgct cgggacggat ttcgtcgtgg aatttcagtc
    13201 ggcgcaaggg aatatgaaca cggcggtagc catcgccaat tatttccgct ctaatccatt
    13261 cgatgtggtg gtcgcgcttt ccacgccttc ggctcaaacg tgcgccaacg ccatccaaga
    13321 cattccggtc gtctttacgg cggtaacgga cccggtcgac gccgagctga ttccggcgct
    13381 gggcaagaat ccgggaaacg tggtgggcgt gagcgatatg acgccggtca ggacgcaatt
    13441 ccaattgatc cgcctcgtcg tcccgaaggc ccgaaaggtc ggcattctcg tcaacccggg
    13501 cgaggcgaat tccctgatca cggtggccca cgcgcgcgag gcttgcgcgg agcttgggtt
    13561 agaactcttg gaaatcgccg gaagcaacac ctccgagatg atcgccgccc tcaccgcgct
    13621 cgtcgggcag gtcgacgcgg tctatgtcgg caccgacaac acggccgctt cctgcgtcga
    13681 atcactgcat cgcatcgcgc aatctaacaa agtgcccctg atcaccggat tcttcaccca
    13741 agaacgagcc aatggcgtga tctcctacgg attcgagtac tataacttgg ggttggacac
    13801 cggaacgatc gtctgggacc tcttgcacgg gaaaccggtg tcggaactcg aatcgcgatt
    13861 gatgaacgcc aacgcgcttc tgttgttgat cgatttagac acagcccaag acatcggact
    13921 gcagataccg gaagcgctga tggctcgcgc ggattacctc gtggtcaatg gcgtccaaac
    13981 acgagtgcgg gagaaatagc tttctggaaa ccaagctttc agactgaaga aacacgttta
    14041 aacaaagcag ggccgctttt tttggcattt cgggtgggac gtcgaaccgc cttaacgcgc
    14101 ccgatcgccg aagaaagcgg cccttttcgt ccctatggtt tttggaaacg ggtttgaata
    14161 cggtcgacca attgcttcgc ggtccgcgtc gcagcaagac ctcccaatcc ggtttcacgc
    14221 aattccatcg gaagcgtctg cccgacgcgg cgcatcgcgt cgacggtttc gtcgaaaggg
    14281 atcacggagg taaccccgga caaggcgagc tccgcggcgc tgatcgcggc ggtcaccgcg
    14341 atcgcgttgc gtttgacgca gggcgcctcg acgaacccgc ccacgggatc acacacgaga
    14401 cccaacaatg atttcagaac caaagccgcc gccgaatcga cggtttccgc atccccgccg
    14461 ctcaagtagg ccaacgcgcc cgcccccatc gccgcggcgc tccctatctc cgcctgacat
    14521 ccgcccgccg ctcccgacag ggtcgcctta gccgcgatca cgtttcctat ggcgctcgca
    14581 aggataaacc caggcagaag gtgggcaaaa gggaaggcgt tctctttcga aagcgcataa
    14641 aatatccccg gaataacccc gctggcgccc gcggtaggac atgcgacgac gcggcccatc
    14701 gatgcgttgc tctcacatat cgagaggctg gcgacgatgg cgacatgggt gaaccgactg
    14761 tggtaaagaa cacctcggtc atgcgctttc ttcgcattgg tacccgtcaa accggttagg
    14821 gtatcctgcg ccttcccgta ttgcttttcc gattcctcga gtatcacgcg caaaaactcg
    14881 ctcagctggg cgcgagtctc ttccgggtta tttcctgatt ctgtcatatc ggccaccaaa
    14941 gccgcttcgt gcaacgggag tccggaatct acggccagtc gtttgatcgc gttgaaggtc
    15001 ataagagacc acccaccctg tcttgcgcga cgtcggagag gctcttcacg tagtacgcct
    15061 ccaggatcac cggtaaagat ttgatttcca acagactccg cagagaaagg tcactgtcga
    15121 gctcgacgat cgtgagcgct ttcgccaaaa gtttgttgat gcgccgaagg tagagattgg
    15181 ccacattcgc gtcgatcttt tccaggatca tattcaaggc tccaggtatg tctttgttga
    15241 cgaggatcag cgtcggatag gccccagaga ggtcgcactc gacgccgttg atcttgatga
    15301 tacgaatcat cccggctcca atagacgagg cccaaatctc ttgcgaatca gcgccgtcgt
    15361 aaagaagcat cttgacggtg ttgggatgaa cctctcccaa atcctcctga tgaaaagagt
    15421 agaccaaacc tcgcgagagc gcaatcccca acgcgtcttt cactgcccgg tccgcgtctc
    15481 cgaaacccat cacgcctgcc aacagcgccc gatcggttcc gtgacccata taggtttgac
    15541 caaaagattg atgaaggaaa aactccgctt tgtccggagt cttgcctaga aaataattcc
    15601 cgaaacgtcc tatccgaagc gcacctaaag tatgagaact cgaagggccg atcataaccg
    15661 gcccccctac gttgactagc gacacagcag acacctcctg atcatttgcc ctccgattgt
    15721 atcatagggc aaaaagattt gcgccctcgt ggcgccaact tccagtcggc agggttttag
    15781 gtttgtcttc ttgggaactc cctgggaacg ccggacttta gtccggcacg gtttcttggg
    15841 cgtctttttg gaaccgccga acttcagttc cgcttgttta cagggaacgc ctgggaacgc
    15901 actgggaacg ccggacttta gtccggcatg atttcttggg cgcctttttg gaagcgcaga
    15961 atttcagttc cgcttgttta ctgggaacgc caacttttta gtcggcatgt ctttccgaag
    16021 agcgtctgaa aaacaaaaaa tccatgtcga actgaagtta agcgttctca agcgctccag
    16081 tggcgtttcg ctgtgctctt tgtgctctct gtgtactccg cgtactctgc gcgaaattcc
    16141 tttttggttt aaaaaaacta tgccgaacta aagttcggcg ttcccagaaa agcgaaacca
    16201 ctccgaactg agtgcagcgt tcccagaaag gcaaagcaca tacggtgcta tagttccgcg
    16261 ctccggtacg ttccgactgc gctcccgttc acgatttcaa gggagatttg acaaaggggg
    16321 ccgaaatgtg gtataacacg ggtaagaatt cgaaaggacc gaatgggtat gatcgagttg
    16381 tcaagtaagc caatttcctc ttcaataagc gtatattatt tttggtataa aacgaggccc
    16441 tctgtaggtt tcgtttgtct ttgagtatac acagaacgaa atgaaagagg gcaccgagag
    16501 aaggtgccct cttttttttc aaaggaggtc ggctatgaga atcagagtcc atttaatgtt
    16561 ttgtctgttt ctttttatag cgctttccct ttttggtatc gtcagaattg gtatcaccgc
    16621 gatcatcgac catccggctt tggacgccat tcgcctggga attatcgatg ggttgatcca
    16681 agacgggttg gttctcgctc aagatttcga tttcgagttt caatcggcgc aaggcaatat
    16741 gagtacggcc ttggcgatcg ctaaccactt caacaccgac gctttcgaca tcatcgtggc
    16801 tatctccacc cccagtgcgc aggcgtgcgc gaacgtgatt aagagtaaac ccgtggtctt
    16861 cagtgccgcg acggaccctg tagctgccgg tttgattacg gttttaggga aaaaccccgg
    16921 gaatgtggtg ggggtcagcg a
//
LOCUS       DAOPXN010000031        16765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3487_length_16765_cov_4.879832, whole genome shotgun sequence.
ACCESSION   DAOPXN010000031 DAOPXN010000000
VERSION     DAOPXN010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16765
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3487_length_16765_cov_4.879832"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /gene="argS"
                     /locus_tag="PKM49_02975"
     CDS             <1..1239
                     /gene="argS"
                     /locus_tag="PKM49_02975"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNY81811.1"
                     /translation="QAVLGDVLSSVFQKAGAALTREYYFNDAGKQMKLLSRSLWVRYN
                     RLCGSEYEIPEGGYHGEYLSGIAEGLYREKGDAYRDRWDDSIEALFTERAKNEIFQMI
                     KRTLESIGIRFDVYYTEGSLFRDGTVDEVLRQLRDREALYEKDQALWFKTSDFEEDQD
                     KVLIRSDGMPTYFLTDIAYHYAKYRRGFSKVYDIWGADHHGHIARMNAAMRALGIPEG
                     FFNVILHQMVSLRQNEEIVKMSKRSAHFVSLDELVQKVGKDAVRYFFAMVDKDSQFEF
                     NIDLALSRSMDNPVYYVQYAVARIHSLFENARNKGLLFAEFQNIDQLDSEDETFLIRD
                     LLLFPEIIREICATQQIHRLTNYLYGLAYAFHNYYTGHVFVDLKQPELSNARLNLASA
                     CQKTIAEGLKLLGVSAPERM"
     gene            1244..2683
                     /gene="mnmE"
                     /locus_tag="PKM49_02980"
     CDS             1244..2683
                     /gene="mnmE"
                     /locus_tag="PKM49_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNY81812.1"
                     /translation="MKSILLRNDIIAAQATPFGVSAIAAVRVSGKDCLPLALEFFSQK
                     TGSASRTLEPASLRPRRLYHGFWKNAEGRWVDEVQIVFYRAPFSFTGEDMVEIFTHGG
                     FLPVKMVLKTLFEKGARPAEPGEFSFRAFVNKKIDLIGAQTINALIHTASPSIEEVLA
                     RQLYDRQEQPIFDIRERLLGLSASLEVAFDYPEDHDAEHPDRIAQAVAKIAQSLEAVI
                     EKAEIAERLNQGLSVVICGRPNVGKSTLLNALLGEDRAIVSEVPGTTRDYLSESFLFQ
                     GLYIRLVDTAGLRETRDTIETLGIRKTLELIDRADLILYLLDPEVDSNEEAPGMWESL
                     RKKRKIILINKRDKYPETDELELAKALKGKIEALTDPNAGPDPVFSISALNRTGLSPL
                     KERMKGILSEVSSVEEGTMWVPAFQIDLLKRAHTILWEGMERFRETGMYETLGQDLRE
                     TVEILDGITGRSFNEDILQTIFSQFCVGK"
     gene            2685..4061
                     /locus_tag="PKM49_02985"
     CDS             2685..4061
                     /locus_tag="PKM49_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HNY81813.1"
                     /translation="MIIRSITNEDLFNFYECPRRFFLMKENGFSSIEMDQSIKMRHFE
                     IARGFFDGKPYIDADWDRQKTLEALLRRESLLFPLLTGRLYEMDVEIKMGGLHWCGDQ
                     YTLLLPFLSREISDRHRTTVFLAHLLLKEVGIPMAEEALFLGRSRNERLKLSENAKPN
                     FIAFMERGFENMGTVFPATSKACISCVFGNQCGVIVPEKRDLPLKALSGVTTSTLGAA
                     ELNGIRTLDDLLKKPLTQDLIEKIKGIEKLKLKAYAFINDLVLIAKGFEAPSEWARGE
                     DLYFDIEADVTPYLFGFRTRDDYLPFLVKEERYYERVTRSMLRFLNLRARNVYHFFEY
                     ERKVLDQLQQKTQISLDRIQLWDVYDILRRNMILPIQHYSLKMVAKWLGYEWKVNLEG
                     QKSIRHFRQWKEKNDHGVLRAILQYNESDCQATEIVKRWLSDPTAFNIPVRYLSKDEE
                     HGIIVGTQ"
     gene            4036..4926
                     /locus_tag="PKM49_02990"
     CDS             4036..4926
                     /locus_tag="PKM49_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNY81814.1"
                     /translation="MELLWELNETDAGRNRGADRLSGKSFFILDMDGTVYLDHTPLPG
                     AVDFVRWLKANQKEFVFLTNNSSKSRETYHKNLSYMGFEVERRNIYTSNTATIEYLED
                     HFPGKRVLLMGTSDAAEEYKKRGVDIALPFDRCMDATYDVAVLGYDTGLSYEKLKNFC
                     LWVRRGLFYISTHPDINCPSLEGPLPDNGAMIELIRASTGRSPDLILGKPQAATLQIL
                     LRQFGREKSETLIIGDRLYTDIRMGAQNGVDTLLVLSGETKEQPSQKGETSESAESAF
                     PTYVSRSLSDILAFVSKREV"
     gene            4928..7090
                     /locus_tag="PKM49_02995"
     CDS             4928..7090
                     /locus_tag="PKM49_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81815.1"
                     /translation="MVNTLRKEFSKFLRDYFSERKDKIVNDETMVSDWKIGHKEVRDL
                     LKRMVLALALESDAYDREKISDEGEAALSPILLGFAEIGEDSKGRHLYVSFDTLKNLL
                     RRVFSKLPTLLNENETYQAFQELTGEKEIDPGRLKKAISTYTPLIRFFENDRGGAATL
                     NLYRFFYPEREAFVSKTLLETTALKTSFLREEKRGLFSKKEQSGAIDPKGIIETAFAL
                     TFSEIGRLIIPLQELYAQKAFRSYFEEDRLPSFIEKELPSFYAHQPTIKKGVLNLFPE
                     NWIDVDSVERELKQKMAEGADPNGWLKQRYPFLNRSEITTLIGLFASPSSSFSPARVS
                     KPELSEKASAAGAKVPVALPKLQQKNTVAEDQGKEKSGSTSVAASDTEPIQTQVPSFG
                     IEKTAQIAEVERPGPPVSTSKPEGVYEAGDRSAGARDEGIGDLIRHLAITDGIVPKTE
                     PKSEAPPQSQRRAENRVEMPKIGDRFPDGREQIEEAPPGPPVDVRFRPEESKASQKSQ
                     SVDALPLESTVDSPSLFNVEPDIEALEIVREPQTEDDHTRKIDFESLESDIDAIDRTD
                     FVADNDREEVEYDPGSGEAESLPEEDSPFAIPFETNLVPPLDFSVAELPEDEAEKTKK
                     VSAEEKSLSGHPPKETEETPASLQGKIITVLTDNEELNAKIGLLSGMTGFELRDSLAY
                     WSDIAVFDRDFEYLIPIAKNTGMKIYLMRDFLRLLGLS"
     gene            7109..9448
                     /locus_tag="PKM49_03000"
     CDS             7109..9448
                     /locus_tag="PKM49_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY81816.1"
                     /translation="MNISHRFFLFFGIILAVLIGVFILVMSSNTLLRNETGKMSQTLD
                     LSVEQIVFNNSLNTLARKTSTTLRQISLLGYADRLENVQRVKGNFVDQLKDIRNQVSA
                     LSQNAVLSKDRGLFSVQEVTQALSDLEREFSSLIALKEREIKAVEQLQASNRVTAALE
                     AERLEVSQRIFEAEKNRDDVLERFLAEYASMTEEYGRKLASPEVIEQINAELDASSLR
                     HFTLYEFERLWDPKYISGLRQLQRFNQMKLHIRDFMSLAVQREDFYALLQEDVSKISA
                     DVDSQTAIGYFMIKLLEALTVKKSLSLFSEKAQAYLLLHQNAAKANLAYTDALKESTA
                     YQNEVFDYERQSLELINAELSPMMERLDSILSKNADDARAALLDLTKRTQNDNQALSE
                     KVNSQMRVILLLTLGAIFVVILVLAFLYLGALRPVAKLSSLSQKLSQLELGIVFPDRH
                     RKDEIGVLENSLKKMVQTIRNTLQKTKSVSDDLVGGSEKVLVSVKKNREISGEIRSKI
                     EEINRAIQESQANLQAVSTHTTELTRDSERLVSDVQALIATVNQRVEGTEREQQAILA
                     TTAKAESIGEEVAGNISSIENLRSITGEIETFVDKILNIAEQTNLLALNAAIEAARAG
                     DSGRGFAVVSEEVKKLAEEANTTSKDIREKLATISDMIDRVVDSSASSNEKVSSMFAE
                     TASIGRVIQEMVTSFKDATGAMVQTLRSIGTQNEQVISLSKQADTIVERFKQLFIQIN
                     SMNASFNDSSKAVEEFSALSFQLVEMSKNLSESVRVFKF"
     gene            9477..10358
                     /locus_tag="PKM49_03005"
     CDS             9477..10358
                     /locus_tag="PKM49_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81817.1"
                     /translation="MKKATIIAGIVVVLLVFLTVVFQNRRITLTVTTHPFVKTEELLL
                     AGFPYPIVPIYSAKDDFLTEVPKKTFEKVSTGDPIAVFEKIQEDFNYQKAQADYARSL
                     LQDGLAVQKEKEVAMQLALNELKNTTTLSPINGYIIRSFAIENTFVNKGTLLFELIPE
                     DFGFLIPVEKGLQERLKSMDAITLTFPRLGLSVSLTEMNYIYRDNQWCLSVPGSRFKG
                     HIPLKEETCTLYVYYPANDLAWIPARFLDSGKVQREDGTWIPVEVFPVPDEESATNDG
                     LVLVKGLTDGMKITGKR"
     gene            10364..11452
                     /locus_tag="PKM49_03010"
     CDS             10364..11452
                     /locus_tag="PKM49_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNY81818.1"
                     /translation="MAVIDISKLILHAYQLNASDVHLSTNENPVFRIFGELKRLKEVE
                     PFSSNDLQKSLEILFGQIGFTPPTNKKEVDFSFGVKDLIRIRANVYFERRNYAAAFRI
                     ITKKIRKLADLNVPILLRELAERDRGMILVSGPTGSGKSTTIAAMIEHVNESKPLHIL
                     TIEDPVEYVFENKMSIIHQRELGLDTLSFADGLKYALRQDPDVILVGEMRDLDTMELA
                     LTAAETGHLVLATIHTNSAPTAPERVIDVFPAHQQKQVGLQLANSLIAVIYQRLVRQV
                     GGKGFAPVLEIMMVTDAIRNLIREGKLHQIESLMDTGKKYGMISFDYALIDAVKQKKI
                     SKEDAFLYCRSVEKMTKELGPLTEALRE"
     gene            11466..11912
                     /locus_tag="PKM49_03015"
     CDS             11466..11912
                     /locus_tag="PKM49_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HNY81819.1"
                     /translation="MSITVFNPFSPTFLQALTHTAPIHEIPVDVLAYVGDAVFNLHCV
                     LLSIGDGRKEVSKASLAASLWKSAEGQNRFLHSLIDGLNEEEAAVVKRGRNSKGAKKR
                     GNDPSYRNSTGFEALVGYLLLSKQETRLQEILDALTRFCSESIPIP"
     gene            11974..14589
                     /locus_tag="PKM49_03020"
     CDS             11974..14589
                     /locus_tag="PKM49_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81820.1"
                     /translation="MDISRIQVNQALREERPGSTEGVTGKVLSRQNNTLEILVGDNVV
                     KVEARAAGKIVPGDVVQVFFGEDTLRTARLGQERPNPVTAKLIDVFSLSFPFPLDENT
                     TRIVEGFSDSEKIGFSRLVSEINRIVGSLLQETLAQGALETAEGTRDSFFSVNTDELR
                     SSIVELALRVKNTGKAWQELPQRIKEEIVKAALIDPLLKKAELRIGGAIAPSIADKAG
                     KEVSASDPHRSDEKHATPPPDRPGSETRAVSAKSIPFTQDSTPASETEGAPEIAREGV
                     AKESIFARLRPSSTFLSSLSEHKTTAARQDPDSGFRPSAPYRVDLPSKAIWSSPPLAE
                     PITVTPGLHTAPAEADEVTKTQELASPIPDLNGSIESKVVDSNRQEIVGSPLVGDDQA
                     DALLVRLVKALKEVLSEPTQMREYYERKEKLADNTPLRIPSPTGRIAPTVSPNTSTES
                     LEPQDPKAEESQTPTKVPSRAFADGAAPLSPLARKVSNLLDARAAVIGKEDRQLLETI
                     LKILQKPEVSSEAPTAPVKAFAKVLTDPPDPGSLWRRVISRPELSALSERITPEVGSR
                     WTQMIVSLSRETSKLGFFGEQTFQRSLSTLFENLIRPEVAHRLAETGTPVEVIERTLR
                     DLKEVFSSIAEKERPIEPRSEAAASSEARDPASTPSRAKEAEGESRPTTLQRAPSSES
                     DRIQEGLKRPSEGLERAPLQTSSTAEAKGVSVGREMPLPETKEGRPLPFQPLDRVLHI
                     GNNAQYDMIYAGFFDLNGGVFRVDLFHNRKPTGGYQADDYYRIIIEAETERLGTVLVD
                     TFSSNEQLDIVLYTEPRHGPFLTTHSHKLIRRLREDGFSVRLFQIKSLQEKDRVIAQK
                     AKMISGTERGFSRFA"
     gene            14582..14914
                     /locus_tag="PKM49_03025"
     CDS             14582..14914
                     /locus_tag="PKM49_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNY81821.1"
                     /translation="MPDNRLTKKKPPVYQTPIDQVTRAVALRFDEQTDSVPLVIAQAK
                     GEIAKRIVELALEYDIPIYSDSGLVEQLLELDYYSAIPPHLYKAVAQVIVYVYQLGGQ
                     KTKNGTDF"
     gene            14898..16253
                     /locus_tag="PKM49_03030"
     CDS             14898..16253
                     /locus_tag="PKM49_03030"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNY81822.1"
                     /translation="MEPIFKTMDDFYSFLYESRPHQQIKLGLERIFKLSRQLGHPERA
                     FRSIHVAGTNGKGSVTRMISAFLTDMGYTVGANYSPHLVNFSERITLNEEPVSDEDLL
                     RVLNAVYPAIERMDRLDQKMKPSFFEIVTLLAFEYFREKKADFASIEVGMGGRLDASN
                     ILRPEVCVITSIGFDHMKSLGNTLAKIAGEKAGIIKKKVPLVCGAISAEAAATIRSRA
                     KKMESPAFCLGEGYSYYPRLCAIDDNRFDYFEPGYALKDISVRLNGRHQFLNGATAIC
                     AYRRLCDRTGIPYREETVRSVLRKISWPARFERIATAPDIIIDGGHNPPAFETLVKAW
                     RDYYGKQKAIFVTGILSDKDYPKMIEVIAPILSHVIVTEPKAGDRVRIEEVAEAFRER
                     LEGVQTELIKDHREACVRALALSGGEKPILSAGSLYLVGYIKDTFLEYLKPVSASAAQ
                     R"
     gene            16292..>16765
                     /locus_tag="PKM49_03035"
     CDS             16292..>16765
                     /locus_tag="PKM49_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81823.1"
                     /translation="MEQRVYIGSRSPLAQSSVPFFFVDTAQDYDDFESSYPGKYNLVF
                     YRRLPVLVDTNGRILFWRCQRDLRSERVFSTIDFSIGLLRRDEIYRVGAYAFLIGTGL
                     QFLCVFDYGARAFSVPRALLEMVSLEVQTHLIYLLYTPEGITYRGFFYMKEQKCIH"
BASE COUNT         4306 a         4180 c         4551 g         3728 t
ORIGIN      
        1 caagccgttc tgggcgacgt tctgtcttcg gtattccaaa aggccggcgc ggcactcacg
       61 agagaatact acttcaacga cgcgggaaaa cagatgaaac tgctctcacg ctcgttatgg
      121 gttcggtaca accggctctg tggaagcgaa tacgagatcc cggaaggagg ttatcacggc
      181 gaatacctat ccggtatcgc cgaaggtttg tatcgagaga agggcgacgc atatcgcgac
      241 cgatgggacg attcgataga ggctctcttc acggaaagag cgaaaaatga gatatttcaa
      301 atgataaaac ggacgttgga gtcgatcggc atccgtttcg atgtgtacta tacggaaggt
      361 tccctatttc gagacggcac cgtagatgag gtcttgcggc aattgaggga ccgcgaggcg
      421 ctgtatgaga aagatcaagc gctctggttc aaaacgtcgg atttcgagga agaccaagac
      481 aaagttttaa tacgttccga tggcatgccc acgtatttct tgaccgatat cgcctaccat
      541 tacgcgaagt accgccgggg cttttcgaag gtatacgata tctggggcgc cgaccatcac
      601 ggacacatcg ctcgtatgaa tgcggcgatg agggccttgg gcataccgga gggttttttt
      661 aatgtcattt tgcaccagat ggtctctttg cgccaaaacg aagagatcgt caagatgtcc
      721 aaacggtccg cccacttcgt ctcgctcgac gaattggttc agaaagtcgg gaaggacgcg
      781 gtccgatact tcttcgcgat ggtggacaaa gacagtcagt tcgagttcaa catcgatctc
      841 gcgctgagcc gctcgatgga caatccagtt tactatgtcc aatacgcggt cgctcgaatc
      901 cattcgctat tcgaaaacgc ccggaataag gggctccttt tcgctgaatt tcaaaacata
      961 gaccagctgg actcggaaga tgaaaccttc ctcattcggg atctgttgtt gtttcccgag
     1021 atcatacgag aaatctgcgc cactcaacaa atccatcggt taaccaacta cctttacgga
     1081 ttggcctatg ccttccacaa ctactacacc ggccacgtat tcgtggacct caaacaaccg
     1141 gagctatcca atgcgcggct aaatctcgcc tccgcgtgtc aaaagacgat agcggaaggc
     1201 ctgaagcttc tgggagtcag cgctccggaa agaatgtgaa aatatgaaaa gtattttatt
     1261 gcgcaacgac attatcgctg cgcaagccac gccgttcgga gtttccgcga tagcagccgt
     1321 gcgggtcagt gggaaagact gcctacccct cgcgctcgag tttttctctc agaaaacggg
     1381 aagcgctagt cggaccctcg agccggcttc ccttcgccct cgacgccttt atcacggttt
     1441 ctggaagaac gcggagggcc ggtgggtgga cgaagttcag atcgtgttct accgggcccc
     1501 tttttctttt accggagagg atatggtcga gatttttacg cacggcgggt ttctcccggt
     1561 gaagatggtt ctgaagactt tgtttgaaaa gggtgcccgt cctgcggagc cgggcgaatt
     1621 ttctttccgg gccttcgtga ataaaaagat agatctcatc ggcgctcaaa ccatcaacgc
     1681 gcttatccat acggcctctc cttcgataga ggaagtgttg gcccgccagc tttacgatcg
     1741 gcaagagcaa ccgatctttg atattcgaga acgccttctc gggttgtccg cttcgctgga
     1801 ggtggccttc gactatcctg aagaccacga tgccgagcat ccggacagaa tcgcgcaggc
     1861 cgtcgccaaa atcgcccagt cgctggaagc cgtcatcgag aaagctgaga tcgccgaacg
     1921 cttgaatcag gggttgagcg tcgtcatctg cggccgaccc aacgtgggca aatcgacgct
     1981 tctaaacgcc ctgctgggag aagatagagc catcgtgtcc gaggtaccgg ggacaactcg
     2041 ggactatctc agcgagtctt tcctctttca gggtctatac atccggttgg tcgatacggc
     2101 cggtttgcgc gaaacgcgcg acacgatcga aacactcggt atacgaaaga ctctggagct
     2161 catcgaccga gcggatttga tcctctatct gctcgacccg gaggtcgact cgaacgagga
     2221 agcgcccggg atgtgggaaa gcctgcggaa aaagcggaag atcattctga tcaacaagcg
     2281 ggataaatat cctgaaaccg atgaactgga attggcgaag gcgttgaaag gaaagatcga
     2341 agccttaacc gacccgaacg ccggaccgga tccggttttc tctatctcgg ccttgaaccg
     2401 aaccggctta agccccctta aggagaggat gaagggcatc ctttctgagg tctcgagcgt
     2461 agaggaggga acgatgtggg ttcccgcttt tcagatcgat cttttaaaac gggcgcacac
     2521 gattctatgg gaaggaatgg aacgatttcg agaaacgggg atgtatgaga cactcgggca
     2581 ggatctgcga gagaccgttg agatcctgga tgggatcacc ggtcgctcat tcaacgaaga
     2641 tatccttcaa acgattttca gccaattctg cgtagggaaa taaaatgatc atccgcagca
     2701 taacgaacga agaccttttc aatttctacg aatgccctcg gcgtttcttt cttatgaaag
     2761 aaaacgggtt ttctagtata gaaatggacc aatcgatcaa gatgagacat ttcgaaatcg
     2821 cccggggctt ttttgacggg aagccgtaca tagacgctga ttgggatagg cagaagaccc
     2881 tcgaggcctt gcttcggcga gagagcctgt tgttcccgct cctcacaggc cgactctacg
     2941 aaatggatgt ggagatcaag atgggcgggc tccattggtg cggtgaccaa tacaccctat
     3001 tgctgccctt tctctcacgg gagatttcgg accgacaccg gacgaccgtc tttttggccc
     3061 acttgctcct caaagaggta gggattccga tggccgaaga ggcgctgttt ttgggccggt
     3121 ccagaaacga gcgtctgaag ttgagcgaga acgcgaaacc gaattttatt gcctttatgg
     3181 aaagaggttt cgagaatatg ggaacggttt ttccggcgac ttctaaagcc tgtatttcgt
     3241 gcgtgtttgg gaatcaatgc ggcgtgatcg tcccagaaaa gcgcgacttg cccttgaagg
     3301 ccttgtccgg agtgactacc tcgacgctcg gggctgccga acttaacggc atccgtacgc
     3361 tagacgacct gttgaaaaaa cccctgacac aggacttgat agaaaaaatc aaaggcatcg
     3421 agaaactcaa actcaaggca tacgccttta tcaacgatct ggttttgatc gccaaaggtt
     3481 tcgaagcgcc atcggagtgg gcgaggggag aggatctgta cttcgacatc gaggcggatg
     3541 tcacccctta tctcttcgga tttcgtacgc gggacgacta tcttcccttc ttggtcaagg
     3601 aagagaggta ttacgaacgc gtgacgcggt cgatgttgcg ctttttgaac ttgcgtgcga
     3661 ggaatgtgta tcattttttc gaatatgaaa gaaaagtgtt ggatcagtta cagcaaaaaa
     3721 cacaaatttc gctggatcga atccagctct gggacgttta tgatatactt aggcggaaca
     3781 tgatccttcc tatccagcat tactccctga agatggtggc aaaatggttg ggatatgaat
     3841 ggaaagtgaa tctggaagga caaaaatcga tccgacactt tcgccaatgg aaagagaaga
     3901 acgatcacgg ggtcttgcgg gccattttgc agtacaatga aagcgattgc caagccacgg
     3961 agatcgtcaa gagatggcta agcgatccga ccgcgttcaa tataccggtg cggtacctgt
     4021 cgaaggatga agagcatgga attattgtgg gaactcaatg aaacggatgc agggagaaac
     4081 cggggggccg atcgtttgtc cggcaagagt ttttttattt tggatatgga cgggacggtc
     4141 tatttggatc acactccctt accgggcgcc gtagatttcg ttcgttggct gaaagccaat
     4201 cagaaggaat tcgtgtttct gacgaacaat tcctcgaaaa gcagggagac ctaccacaaa
     4261 aacctatcct atatggggtt cgaggtcgaa cgccggaata tatacacctc caataccgcg
     4321 acgatagagt atctcgaaga ccattttcct ggtaagcgcg tcctgttgat gggaacgtcc
     4381 gacgctgccg aggagtacaa aaaacggggc gttgatatcg ctctaccctt cgatcgatgt
     4441 atggacgcca cttatgatgt ggcggttcta ggatacgaca ccggattatc ttacgagaaa
     4501 ttaaaaaact tttgcctgtg ggtaagaagg ggattattct atatcagcac ccaccccgat
     4561 atcaactgtc cttctttgga gggacctttg ccggacaacg gagctatgat cgagttaatc
     4621 cgcgcctcta cgggacgtag tccggacttg attctgggaa aacctcaggc agcgacgctc
     4681 cagatactct tgcgtcagtt tgggagagaa aagtccgaga ccctgatcat aggcgatcgc
     4741 ctttacacgg acatccgaat gggcgcgcaa aacggggttg atacgttgtt ggtgctttcg
     4801 ggagaaacga aagaacaacc gagccagaag ggtgaaacct ccgaaagcgc ggagtcagcc
     4861 ttccccactt acgtgtcccg atcgttgtcg gatatcctgg cgttcgtctc gaagagggag
     4921 gtatagaatg gtaaacacac ttagaaaaga attctctaag tttttgaggg attacttttc
     4981 cgaaagaaaa gacaagatcg tgaacgacga gacgatggtg agcgattgga aaatcggtca
     5041 caaagaggtg agagacctat taaaaaggat ggtcctcgcc ctcgcgctcg agtcggatgc
     5101 ctacgatcga gaaaagatat ctgatgaggg agaggcggcc ctcagtccta tccttttagg
     5161 gtttgccgaa ataggcgaag atagtaaagg gcgccatctt tacgtttcct ttgatacctt
     5221 gaaaaacctg ttaaggcgtg tattttccaa actcccaacg cttttgaacg aaaatgagac
     5281 ctatcaagct ttccaagagt tgacagggga gaaggagatc gaccccggcc gcttgaaaaa
     5341 agccatatcg acctatacgc cgctgattcg gttctttgaa aacgatcgag gaggcgcagc
     5401 gacgctcaat ctctatcggt tcttctaccc agagcgagag gctttcgttt cgaagaccct
     5461 tctagaaaca accgctctca aaacctcctt cttgcgagaa gaaaagaggg gactgttttc
     5521 caaaaaagag caatcgggtg cgatcgatcc caaagggatc atagagaccg cttttgcgct
     5581 cactttctcg gagatcggcc ggttgatcat ccctcttcaa gaactctacg cgcagaaggc
     5641 gttccgtagt tatttcgagg aggaccggct gccatctttt atcgaaaagg agctgccatc
     5701 tttttacgcg catcaaccga ccatcaaaaa gggtgttctc aatctctttc cagaaaactg
     5761 gatcgacgta gatagtgtag agagagaact caagcaaaag atggcagaag gagcggatcc
     5821 taacggttgg ctgaaacaga gatacccgtt tctcaatagg tccgagatca cgaccttgat
     5881 cggcctattt gcatcaccct catcgtcgtt tagccccgcg agagtgtcca aacctgaact
     5941 ttcagagaag gcctctgcgg cgggtgcgaa ggtaccggtc gcgcttccga aactccaaca
     6001 gaagaataca gtggctgaag accaagggaa agaaaaaagc ggatcgacct ccgtcgcggc
     6061 atcagatacg gaaccaatcc aaactcaggt accctcattc ggaatagaaa agacggctca
     6121 aattgcagaa gtcgagcggc ccggaccacc ggtgtccact tcgaaaccgg agggggtcta
     6181 tgaagccggc gatcggagcg ccggggcacg tgacgaaggt atcggcgact taatccgcca
     6241 cttggcgata accgatggga tcgttcctaa aacggaaccg aagagcgaag cgccacctca
     6301 gtctcaacgt cgggctgaaa accgtgttga aatgccgaaa atcggagacc gatttccgga
     6361 cggccgagaa caaatagagg aggccccgcc cggtccaccc gtcgacgtga ggttcagacc
     6421 tgaggagtcg aaggcttcgc aaaagtccca aagtgttgac gcgctcccgc tagagagcac
     6481 ggtagactcc ccctctctct tcaatgtgga gccagacatc gaagcgctcg aaatcgtacg
     6541 tgaacctcag accgaagacg accatactcg aaagatcgac ttcgagagcc tcgaatccga
     6601 tatcgacgcg atcgatcgaa ccgacttcgt agcggacaac gatagggaag aggtcgaata
     6661 cgatccgggc tccggagaag cggaaagcct accggaagaa gattccccct tcgcaatacc
     6721 gtttgaaacg aaccttgttc ctcctttgga tttttcagtc gccgaacttc cggaagacga
     6781 ggcggagaag actaaaaaag tctcagcgga ggaaaaaagc ctttcgggtc atccgccgaa
     6841 ggaaactgaa gaaactccgg ccagtttgca gggaaagatc atcaccgtgt taactgataa
     6901 cgaagaattg aacgcgaaga tcgggctact cagcggaatg accggttttg aattgcgcga
     6961 cagcctcgcg tattggtcag atattgccgt tttcgaccgt gatttcgagt acctgatacc
     7021 gattgcgaaa aataccggga tgaagatcta cctgatgcgc gatttcctgc gcctattggg
     7081 tctatcctaa aaattaggag gaagtaaggt gaacatttca catcggtttt tcctgttctt
     7141 cgggatcatt ttggcggtcc tgatcggggt attcatattg gtaatgagct cgaacacgct
     7201 gttgagaaat gaaactggaa agatgtccca aaccctggat ctcagcgtag agcagattgt
     7261 ttttaacaat tcactaaaca cgctggcgcg aaaaacatca accactttac gtcaaatctc
     7321 tttgctcggt tacgcggacc gtttagaaaa cgttcagcgt gtgaagggca atttcgtaga
     7381 ccaactcaaa gatatacgga atcaggtgag cgctttatct caaaacgccg tcctttcgaa
     7441 agaccgcggg cttttttctg ttcaggaggt cacccaagcc ctttcggacc tcgaacgcga
     7501 gttttcgtcg ctcattgctt taaaagaacg ggagatcaag gcggtcgaac agcttcaagc
     7561 gtccaatcga gtaactgcgg ctctggaagc cgagcgcttg gaggtatcgc aacgaatttt
     7621 cgaagcggaa aaaaaccggg acgacgtttt ggaaagattc cttgccgaat acgcttcgat
     7681 gacggaagag tatggtagaa aactggctag ccccgaggtg atagagcaga tcaacgccga
     7741 actcgacgcc tcttcgctac gtcacttcac cctctacgag ttcgaaaggc tttgggatcc
     7801 aaagtatatc tcaggactaa ggcagctaca acggttcaac cagatgaaac tgcatatccg
     7861 agatttcatg tctctagccg ttcagcggga agacttctac gcgctcttgc aggaagatgt
     7921 gtcgaaaatc tcggcggatg tggatagtca gacggcgatc gggtatttta tgatcaagct
     7981 gttggaggcg ctgacggtca aaaaatctct ctctttgttt tcggaaaaag cgcaagccta
     8041 tctgttgttg caccaaaacg cagcgaaggc taacttggct tacaccgatg cgttgaagga
     8101 atcgaccgcc taccagaacg aggttttcga ctatgagaga caatctctcg agctcatcaa
     8161 cgcggaacta tcgccgatga tggagcgctt ggactctatc ctatccaaaa atgcggacga
     8221 cgcccgggct gcgcttctgg acttgacgaa aagaactcaa aatgacaacc aggccctttc
     8281 agaaaaggtg aactctcaga tgcgggtcat acttctgctc accttgggcg cgatcttcgt
     8341 ggtgatctta gtcctggctt ttctgtattt aggcgcgcta cgacctgtcg caaagctatc
     8401 gagcctctca cagaaactct cgcaactcga gttaggaatc gtcttcccag ataggcatag
     8461 gaaggatgag atcggagttc tggaaaattc tttaaaaaag atggtgcaaa ccatccggaa
     8521 caccttacaa aaaacgaagt cagtgtcaga cgatttagtg ggcggttccg aaaaggtcct
     8581 cgtttcggtc aaaaagaacc gcgaaatctc cggcgaaatc aggtccaaaa tcgaggagat
     8641 caaccgcgcg atccaggaat cccaagccaa cttacaagcc gtttctaccc atacgaccga
     8701 gttgacgcgc gatagcgaac ggttggtatc ggatgtgcaa gcgctgatcg ccaccgtgaa
     8761 ccagcgggtc gaggggacag agagagaaca gcaggccata ctggcgacga cggccaaagc
     8821 cgaaagcatc ggtgaagagg tcgccggtaa catctcgagc atagagaatc tcagatcgat
     8881 caccggtgag atcgaaactt tcgtagacaa gatcctgaac atcgccgagc agaccaactt
     8941 gctcgcccta aacgccgcga tcgaagcggc gcgagcgggt gactctggga gggggttcgc
     9001 ggttgtctcc gaggaagtaa agaagctggc ggaggaggcg aataccacct ccaaggacat
     9061 acgagaaaag ctagcgacta tatcggacat gatcgatcgc gtggtcgatt cgtccgcgtc
     9121 cagcaatgaa aaagtgagtt cgatgttcgc agagaccgcg tctataggcc gcgtgatcca
     9181 agagatggtg acctccttca aggatgcgac gggtgcgatg gttcaaacgt tacgtagtat
     9241 cggtacccaa aatgaacagg tcatcagcct ttccaaacaa gcggacacca tcgtcgaacg
     9301 gtttaagcag ctttttatcc aaatcaacag tatgaacgcc tcttttaacg acagttcaaa
     9361 ggcggtcgaa gagttttcgg ccctctcttt ccaactggtg gagatgtcga agaacctttc
     9421 cgaaagcgta cgggtcttca agttctgata cgccggtagg agattagggt gcgggattga
     9481 aaaaagccac gattatcgcc ggtatcgtcg tcgttttgct agtcttttta accgtcgttt
     9541 ttcagaatcg tcgtatcaca ctcaccgtga cgacgcaccc tttcgttaaa acggaggagc
     9601 tgctgctcgc cggcttcccc tacccgatcg ttccaatcta tagcgcgaaa gacgatttct
     9661 tgacggaggt ccccaaaaag accttcgaaa aagtgtcgac cggcgatcct atagccgtgt
     9721 tcgaaaaaat acaagaggat tttaactatc agaaagcgca ggcggactac gcgcggtcgc
     9781 ttctacagga tggcctggcc gttcaaaaag aaaaagaggt cgcgatgcag ctcgcgctaa
     9841 acgaactgaa aaacacgacg acgcttagcc cgatcaacgg ctacatcata cgttctttcg
     9901 cgatagagaa tacttttgtg aacaaaggga ccctgctgtt cgagctgata cccgaggact
     9961 tcggcttttt gataccggtc gaaaaagggc tccaggagcg actaaaatca atggatgcga
    10021 tcacattgac cttcccacga ttggggttgt ccgtgtcatt aaccgagatg aattacatct
    10081 atagagacaa ccaatggtgc ctttctgtcc cgggttctcg gtttaagggc catattccct
    10141 taaaagaaga gacttgtacc ctctatgtgt attatccggc gaacgatttg gcgtggattc
    10201 cggcgcgctt tctggattcc gggaaggttc aacgagagga tggcacgtgg ataccggtcg
    10261 aggtctttcc ggttccggat gaagaaagcg cgacgaacga tggcttagta ctcgtcaaag
    10321 gattgactga cgggatgaaa ataacaggca agaggtgagt gccatagcgg tgatcgatat
    10381 ctccaaactt attctgcacg cgtatcagtt aaacgcctcg gacgtccatt tgagcaccaa
    10441 cgaaaacccg gtctttcgta ttttcggaga gctgaagcgc ctgaaagagg tggaaccctt
    10501 ttcttccaac gacctgcaga agagtctcga gattttattc gggcagatag ggtttacgcc
    10561 gcctaccaat aaaaaggagg tagacttctc ttttggcgtg aaagacctaa tccggattcg
    10621 agcgaacgtc tattttgaac gccgcaacta cgcggccgcg tttcgcatta tcaccaaaaa
    10681 gatccggaag ctggcggact tgaacgttcc gatcctgctg cgggaattag cggagcgcga
    10741 ccgtgggatg attttggttt cgggcccaac cggaagcggg aagtccacga ccattgcggc
    10801 gatgatcgag cacgtcaacg aatcgaagcc tctgcacatc ctcacgatcg aagaccccgt
    10861 ggagtacgtc ttcgaaaaca aaatgtccat catccaccaa agggaactcg ggttggatac
    10921 cctatccttc gccgatgggc tgaagtacgc gttacggcag gatcctgacg tgatactcgt
    10981 cggagaaatg cgcgatctgg atacgatgga actggcgttg acagcggcgg agaccggcca
    11041 tttggtttta gccacgatcc acacgaactc tgctccaacg gctccggagc gagttataga
    11101 cgttttcccc gcgcaccagc agaaacaggt cggcctacaa cttgccaact ccttgatcgc
    11161 agtcatctat cagcgattgg tgcgccaggt gggcggaaaa ggatttgcgc cggtcttgga
    11221 gattatgatg gtgacggacg ccatccgcaa tctcattaga gaaggcaaac tccatcagat
    11281 agaaagtctg atggatacgg gtaagaaata cggcatgatc tctttcgatt acgctttgat
    11341 cgatgcagtt aaacagaaga agatctcgaa ggaagacgcg tttttgtact gtcggagcgt
    11401 ggaaaagatg acgaaagaac tgggtccgtt gaccgaagcg ctccgggaat agggggcttc
    11461 gtcgtttgtc cataaccgtt tttaacccat ttagtccgac tttcttacag gctttgaccc
    11521 ataccgcccc gatccacgaa atccccgtgg atgttctggc gtacgtcgga gatgccgtgt
    11581 ttaacctgca ttgcgtgttg ctttcgattg gagatggcag aaaagaggtg tcgaaagcca
    11641 gtcttgccgc ttctctatgg aaaagcgccg aaggccagaa tcgcttccta cattccttga
    11701 tagacggctt gaacgaagaa gaggcggcgg tggtcaaacg cggacggaac tcgaaaggcg
    11761 ccaaaaaacg cggcaacgat ccctcctatc gcaacagcac gggcttcgag gccctggtcg
    11821 ggtaccttct tctgtccaag caagagacac ggctccagga gatattggat gcgctcaccc
    11881 gtttctgttc cgaatcgatc ccgattcctt gaataaacga tccgagaggt cgataaagag
    11941 gaggtagtcc tgcttggaaa gatgggtgaa ggaatggata tttcgagaat acaggtcaac
    12001 caggcattaa gggaagaaag gccgggatcg actgagggcg tgaccggaaa ggtcctttcc
    12061 cgtcagaata acacgcttga gattttagtc ggagataacg tcgtgaaagt ggaagcgcgg
    12121 gccgccggga agatcgtgcc gggagacgtg gtgcaggttt ttttcggaga ggacacccta
    12181 cgaaccgccc gcctaggtca agaacggcca aaccctgtga ctgcgaagtt gatagacgtc
    12241 ttttccctgt cttttccttt tcctttagac gagaatacga cgcgtatcgt cgaaggtttt
    12301 tctgactcgg agaagatcgg gttttcgcgc ctggttagcg aaataaaccg aatagtgggc
    12361 tcacttctac aggaaacatt ggctcaaggc gctctggaaa cggccgaagg gactcgggac
    12421 tccttttttt cagtcaatac cgacgagctg aggtcctcta tcgtggagct cgcactgcgc
    12481 gtcaaaaata cgggaaaggc ctggcaagaa ttaccgcaga gaataaaaga agagatcgtg
    12541 aaagcggcgt tgatcgatcc tttattaaaa aaagcggagt tgagaatagg aggcgccatc
    12601 gccccctcga tagccgataa ggccggtaaa gaggtttcgg cttccgatcc tcatcgttcg
    12661 gatgagaagc acgctacccc tccccccgat cgaccgggtt ctgaaacgcg cgctgtgtcg
    12721 gctaagtcaa tcccttttac acaagactcg acacccgcat cggaaacgga aggcgctcct
    12781 gaaatagcgc gtgaaggcgt agcgaaagaa tcgattttcg cgcggctgcg tccctcttcg
    12841 acgtttttgt catctttaag tgaacataag acgactgcgg ccagacaaga ccccgatagc
    12901 ggctttcgcc catcggcgcc ctatcgcgtg gacctaccct cgaaggcgat ttggtcttct
    12961 cctccgctcg cagagccgat tacggtgact ccgggtttgc atacggcacc ggcggaggcc
    13021 gacgaggtaa cgaaaacaca agaattggcg tcaccaatac cggacctcaa tggttcgatc
    13081 gagtcgaaag tcgttgactc gaatagacaa gagatagtgg gaagcccgtt ggtaggggat
    13141 gaccaggcgg acgcgctctt ggtccgactc gtgaaagctt tgaaagaagt tctttcggaa
    13201 cccactcaga tgagagaata ctacgaacgc aaagagaagc tggcggataa tacgccgtta
    13261 cggattccgt ctcccaccgg gcgaatcgcg ccaacggttt cgccgaacac ctcaaccgag
    13321 agcctcgagc cgcaggaccc gaaagccgag gagtcacaaa cccctacgaa agtgccgtct
    13381 cgggcgttcg ccgacggggc cgctcctctc tctcctttgg cgaggaaggt ttcgaattta
    13441 ctcgacgcgc gcgcggcggt gatcggcaag gaagaccgtc aactactgga aacgattctg
    13501 aaaatactgc aaaaacccga ggtctcgagc gaagcgccta ccgccccggt taaggctttc
    13561 gcgaaggttc tgacggaccc tccggacccg ggctctctct ggagaagggt gatttcacgc
    13621 cccgagctct ccgcgttatc cgaacggata acgcccgaag tcggttcgcg atggacgcag
    13681 atgatcgtct cgctttcacg agaaacgtcg aaactggggt ttttcggcga acaaactttc
    13741 caaagaagcc tttccaccct gtttgaaaac ctaatccgac cggaagtggc tcataggctt
    13801 gcggagacgg gtacccctgt agaagtcata gaacggactt tgagggactt gaaggaagtc
    13861 ttctcttcca tcgcggaaaa ggaaaggcct attgaaccgc gttcggaagc ggccgcttca
    13921 tccgaagcgc gagaccccgc gtcaacgcct tcgcgggcaa aggaagcgga aggggaaagc
    13981 cggccgacta cgctccaaag agccccgtcg tcagaaagcg atcggatcca ggagggattg
    14041 aagcgccctt ccgaaggctt agaacgggcc ccccttcaaa cctcttccac agctgaagcg
    14101 aaaggcgtga gcgttgggag agaaatgccc ctgcccgaga cgaaagaggg aagaccgctg
    14161 ccctttcaac ccctcgaccg ggtgctccat atagggaata acgcgcagta tgatatgata
    14221 tacgcaggtt tcttcgattt gaacggcggt gtttttcgcg tcgatctgtt ccacaaccgg
    14281 aaacctaccg gcgggtacca agcggacgac tattaccgga tcatcataga ggcggaaacc
    14341 gagcgcctcg gcacggtcct cgtcgacacc ttttcctcta atgaacaact ggatatcgtt
    14401 ttgtacacgg aaccgcgaca cgggccattc ctgacgaccc attcgcacaa actgattcgg
    14461 cgtctaagag aagacggttt ctccgtcagg ctattccaga tcaagtccct acaagaaaag
    14521 gacagagtga tcgctcagaa agcgaagatg atatccggaa cggaaagggg tttttcgcgc
    14581 tttgcctgac aaccgtttaa cgaagaaaaa gcctccggtc tatcagacgc ccatagatca
    14641 agtcacgcgc gccgttgcct tgcggtttga tgaacaaacg gatagcgttc ctctggtcat
    14701 cgcgcaagcc aaaggagaaa tcgccaagcg tatcgtcgag ctggctttgg agtatgatat
    14761 ccctatctac agcgatagcg ggctggtcga gcaactgctg gagttggact attactccgc
    14821 gatcccgccg catttgtata aggcggttgc tcaagtgatc gtgtacgttt accagttagg
    14881 aggtcagaag acgaagaatg gaaccgattt ttaaaacgat ggacgacttc tactcctttt
    14941 tgtacgagtc gcgtcctcac cagcagatca agctgggact cgaacggatc ttcaagttgt
    15001 cgaggcagct ggggcatccg gagagagcct tccgttccat acacgttgcc ggaacgaatg
    15061 gcaaaggctc tgtcacgcgg atgatctccg cttttctcac cgatatgggg tacacggtcg
    15121 gggcgaatta ttctcctcat ctggtgaact ttagcgaaag gatcacccta aacgaggaac
    15181 cggtctccga cgaggatctg ttgcgcgtcc tcaacgccgt gtatccggcg atcgagagga
    15241 tggatcgcct ggatcagaag atgaaaccca gtttttttga gattgtcacg ctcttggcct
    15301 tcgagtactt cagagaaaag aaagcggatt ttgcttcgat agaggtggga atggggggac
    15361 ggttggacgc ctccaacatc cttcggcccg aggtttgcgt aatcaccagc atcgggttcg
    15421 accatatgaa atccttggga aacaccctgg cgaagatagc gggggaaaaa gccggcatca
    15481 ttaagaagaa ggtcccgctc gtttgtggcg cgatttcagc ggaagcggcc gcgacgatac
    15541 gaagccgggc gaaaaagatg gagagtcccg ctttctgtct cggtgaaggc tattcctatt
    15601 acccccgtct ctgtgcgatc gatgacaacc gttttgacta tttcgaaccg ggttacgcgc
    15661 ttaaagacat ttcagtgcga ttgaacggtc gtcaccagtt cttgaacggg gcgacggcga
    15721 tctgcgccta tcggcggctc tgcgatcgga ccggtatccc ctatcgagag gagacggtac
    15781 gatcggtttt acggaaaatc tcttggcctg cgaggtttga gcggatagcc accgcgcccg
    15841 atatcatcat cgacggcggt cacaatcctc cagctttcga aaccctagtc aaagcatggc
    15901 gggactatta tgggaaacaa aaagcgatct tcgtgacggg tatcctgtct gataaagact
    15961 atccaaagat gatcgaagtc attgcgccga tcctctctca cgtgatcgtg accgaaccca
    16021 aagcggggga cagggtacgg atagaagagg tggccgaggc tttccgagaa cggctcgagg
    16081 gtgttcagac ggagttgatc aaggaccacc gcgaagcctg cgtacgggcg ttggcgcttt
    16141 ccggcggaga aaagccgatt ttgtccgccg ggtcgctcta tctcgtcgga tacataaagg
    16201 atacctttct cgaatacctt aaaccggttt ctgcatcggc tgcgcagaga taaaattgtt
    16261 ctcaggattg gaagggtgaa aaagggagtc tatggaacag cgggtgtaca tcgggtcgcg
    16321 ctcgccgttg gcgcagtctt ccgtcccttt cttttttgtg gatacggcgc aagactacga
    16381 cgactttgag agttcttatc ccggtaagta caacttggtt ttttatcgtc gattgccggt
    16441 cctcgtcgac acgaacgggc gtatactttt ttggcgctgc cagagagacc ttcggagcga
    16501 acgggttttt tcgacgatag acttttctat cgggttgctg cgacgcgatg agatctaccg
    16561 agtaggagct tacgctttct taatcggaac tggattgcag tttctttgtg tcttcgatta
    16621 cggggctcgg gctttctccg taccgcgggc gctgctcgaa atggtgtcgc tagaggtgca
    16681 aacgcacctc atttaccttc tatacacccc ggaagggatc acgtatagag gcttctttta
    16741 catgaaggaa caaaaatgca tccat
//
LOCUS       DAOPXN010000032        16659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3518_length_16659_cov_4.501325, whole genome shotgun sequence.
ACCESSION   DAOPXN010000032 DAOPXN010000000
VERSION     DAOPXN010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16659
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_16659_cov_4.501325"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKM49_03040"
     CDS             <1..528
                     /locus_tag="PKM49_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81824.1"
                     /translation="VLEYLRANPSMLYKSLTPNLTLMPAVWERASKLLYSFSNMQEIK
                     TKGLIPLQLVVGVSVGHEMMEMLFKSDCPTISPMDVIREYSKNEKLRLLVLEKAEAGH
                     MCELARLADAVSILMEKWDMNIVLFALDLPNDTCQMLISRLRSSEVDISDPKMEEALA
                     KLYIKIGDFGLETAI"
     gene            528..1841
                     /locus_tag="PKM49_03045"
     CDS             528..1841
                     /locus_tag="PKM49_03045"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNY81825.1"
                     /translation="MDRSNPPPYPTMADLESALQEGIQSGCLFSYILSSLNRRIDETA
                     EAIAYVSLSPNRKAWSLVVNPILFSQIYETRSKEPRERVLRAILIHEFLHLAHDHFDR
                     GLTLLKSYSLETLNIAADLAVNGCRKHGELSVLEEIGGLFPDRPPFDFPVRLSMEQYL
                     ALLPKTKGCMLVSVPKWLSGFSQTMEELQGLSDNGTPYEGSVYASIRDLASSATLELK
                     KRIGELMQGLQKKPSSDLRSRGFGRGEDVWVTDWVFPRVKNTWMRHLRNVLTGSGSGV
                     ANTRSYRRPHRREGGGILLKVNISSQKEQTLIILDTSGSMMAADYELFMGLIKTLNSD
                     ARSFYVMQWDYEQQTEPVPVTVFMAKKKPEFHGRGGTDMQQAVLVASQRYPQYKKMVV
                     VTDGGTPYFTKDDPSPVPVIWVLTHNAPFNNADGVVVRLREDRDT"
     gene            1868..2962
                     /locus_tag="PKM49_03050"
     CDS             1868..2962
                     /locus_tag="PKM49_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNY81826.1"
                     /translation="MKKEETYWIPGLDQNEHKVLSLSDIGLILRGSLHSAKNANAMSV
                     KGRLSAALNALSITSEMVRVRAGSFKMGHTREDRVEDAIHLGFFSHLVTLTYDYWIGR
                     YPVTCQEYDCFCESIGKAKPRDPFEENEWVPRRYFPVGYLKWGDAVRYCNWLSLREGL
                     KPAFDFDGNLLDTNGNITTEIKNVEGYRLPTEAEWEYAARGGHKRTRDYRFPGTDDLE
                     DLLIPYYDDKPLGQTKPNDLGLFDMSGTIWEWCYDRYFDYTNSTERVDPIGPTKGRAR
                     VIRGCGLDVNYALDRGREIEFNVAYRYESPPISRRSGLRTGFRVARTCDWARGDGQEP
                     AERKFVIPRKQRSHKGHRGHGVKKEIPLEF"
     gene            3625..4656
                     /locus_tag="PKM49_03055"
     CDS             3625..4656
                     /locus_tag="PKM49_03055"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNY81827.1"
                     /translation="MNVNCLSEITEAYLQQKVFPGGVIGFGNADRFSVFPFGKLDEEQ
                     PVCVEALYDVASLTKVVATLPAVFTLVQKGKIDLEDPVSRFLPVPYPSIRVWHLLTHS
                     SGFPPYSDAYKWAANPEEILQEIYRNPWVKEPGKEVVYSCLNFILLKQIVASIVGDYA
                     AYLKEAVFAPLGMNKTGFLPDNQEPIAPTSFRDGVRLRGLVDDELAYYLGGVSGNAGL
                     FSCIEDLCRYAQEWIRPRHILVPSTVALATRPWTSQIPGDPKGLGWALHTSRTSDGSL
                     FSPNSFGHTGFTGTSLWIDPDHGRFVCILTNRCFYDRHTAQSKDAIWEFRRRVAQVAV
                     SIMERGEVV"
     gene            4653..5795
                     /locus_tag="PKM49_03060"
     CDS             4653..5795
                     /locus_tag="PKM49_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNY81828.1"
                     /translation="MKRIIGLMSGTSCDGLDIACCQFVNNAERTELQLEHLESIEYPP
                     DLRDWIRGLTSGKISVAEVAQAHFRLGTLFGDWAFDHIRRHQLEGKVDLIVSHGQTVF
                     HRPFMGRSENEPACTLQIGDGDVIASITGTRVLSDVRVKDMAYGGQGAPMVVWADYVL
                     FRSKEKNVAMQNIGGIGNVTFLPKAGGVESVLAFDTGPGNVLIDEACRTLFERKFDED
                     GEISAKGTVNRPLFNRMREIEEGYVAERPPKSTGKEIRYHQQYRERLWKLREEFGCSP
                     HDFVRTLVALTAWSIGESYRRYLGEVERVILTGGGALNPTLVAEIIAELPGTEIRKLD
                     DDWTAFKEAIAFAVIGNEYLNGHPANVPSATGARKAVTLGKLSLPE"
     gene            5832..7061
                     /locus_tag="PKM49_03065"
     CDS             5832..7061
                     /locus_tag="PKM49_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY81829.1"
                     /translation="MNIAMLTDTYLPHVNGVSTSLFLFRKHLERLGHRVYIVSPVGQK
                     GDPYFFKVMGFRSMQVKDQRIALPNTKRILDFLETNQITHLHSHAPLSMGWTALWLQK
                     RYGFIHFHTYHTLLVDYLHYIPRFFRPTKKVIQEFSKWFCSEVDCTIAPTTEMKNELM
                     AYGMKKPIHVIPTGIDTDLFRTAAPIDLRAKHHLPKESFIALFAGRIAKEKNIVFLLE
                     VVERMIRKGHSFYLVLVGNGPFFEELSEIVRKKRLSENVIFAGEMKREELASYYRSAD
                     CFTFASTTETQGLVVLEALAAGLPVIAVAQKGVKSSLSEGEGCFLTEHPNVDFFVEKA
                     LLLMKDPELRQQMRGKGMEYVQKHWSIDVFAQHVSDLYGQFQKPEQYTPQKMFEFSEL
                     WMWVTGKLRNIQDNIFK"
     gene            7075..7758
                     /locus_tag="PKM49_03070"
     CDS             7075..7758
                     /locus_tag="PKM49_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81830.1"
                     /translation="MTRMPRNVPSFLPLLVVLTGLFSVLAFSLVLYLETDSFLDIIAQ
                     TLSALSFYLQVALFLVWALLLNQNIKLTYLRAEEFIPQADEETSFDLRWLQTNLRKNT
                     VPLWPLALLTVFNTLSLLFDFDLYLLSLASVSLSLFFLYRLLFCSRQLLTLKSNFYSF
                     YKVEGYTDQFQSIFPNRTLLSFTLLVFLTLGFYLFYFFLRFSAELNLYLALDGGYLDR
                     LALPEKPGD"
     gene            complement(7817..8527)
                     /locus_tag="PKM49_03075"
     CDS             complement(7817..8527)
                     /locus_tag="PKM49_03075"
                     /inference="COORDINATES: protein motif:HMM:NF039337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HNY81831.1"
                     /translation="MRFGNHKTVGQDPNYFSLLEAMNADGCVVCRLADRQLERIIDSL
                     RYERIDDYEIRRKIHQSGGFCREHALRILEQGDPLLHAIIYSRLFEQKVETLRSGEDI
                     ASRRSSSDGRKRFGAREGLRAPNGSECLICEGLRELEDRVLPLIPMFYDCDETFRKAF
                     DERGHLCVPHLEAVFSKSKPDASPNGILSAQIRKYDQLARHLREIERKFDYRYCNETS
                     CEDEKKAWRLAVALWTGC"
     gene            complement(8531..10066)
                     /gene="guaA"
                     /locus_tag="PKM49_03080"
     CDS             complement(8531..10066)
                     /gene="guaA"
                     /locus_tag="PKM49_03080"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNY81832.1"
                     /translation="MGKIVIVDYGSQYTQLIARKTRDLRVFSEVVTATSQPNLEDISG
                     LILSGGPFSVYDPRAPRIPDWVKPLIEKEVPILGICYGFQLLTQYFGGRVDRAHSSEY
                     GRMPIVRQGENPLFDGIDRSLTVWMSHGDSVLELPPGFAICALSENEIIASAFHVKDP
                     IYGLQFHPEVNHTEFGDRIFSNFLFKICRCTPDWTLKDFIAIERENLRATIGTQRAVC
                     AVSGGVDSTVAAVLAHQAIGSQLTGVFVDTGLLRKNEDAVVPDLLRRSFGLNLRVVNA
                     RETFLARLAGVVDPEEKRKIIGETFIRVFESEAREIGSPPFLIQGTIYSDVIESAGTL
                     GGGTIKIKTHHNVGGLPQDMRFKIVEPLRFLFKDEVRRAGAILNIPEQILARHPFPGP
                     GMAIRCLGEVTSDRVRILKEVDAIFLQTLHEFGWKEKVWQAFAVLLPLKSVGVAGDQR
                     AYGNVVALRSVDSSDAMTADWSRIPYHILDEAARRIINRVAEVGRVVYDITAKPPATI
                     EWE"
     gene            complement(10068..10907)
                     /locus_tag="PKM49_03085"
     CDS             complement(10068..10907)
                     /locus_tag="PKM49_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HNY81833.1"
                     /translation="MAKFDQLIITAAICGAEVTRNETPYLPLTPEELSDEAKKCYDAG
                     ACIVHLHVRDPEGNPTQDAAIFKRTVGLIRQKAPNLIVQVSTGGATWMKAEERIQSIE
                     SAPDMATLSTGSCNFGDDLFLNSKGMVLEFAKKMSERAIMPEFECFEIGHIHFANWLV
                     QKGHVVWPHFHYDFVMGVPGAIAATVPLLVRMREEIPAEASWTVAGIGRMELPMATVA
                     LAMGGHVRVGMEDNLYYSKGVLAKSNAELVARIVRIAKELGRPIATPDEVRDYFHLQK
                     KSL"
     gene            complement(10920..11315)
                     /locus_tag="PKM49_03090"
     CDS             complement(10920..11315)
                     /locus_tag="PKM49_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HNY81834.1"
                     /translation="MKKAMIRLRMSEADAHYAGGLVDGAKILQLFGDVATELLIVLDG
                     DEGLFRAYDSIEFLAPVYAGDYLEITGEIVETGKSSRKMTFQARKVITNASIPEQPSA
                     CVVLDEPILVCKASGTCVVPVSKQRYRDM"
     gene            complement(11281..12663)
                     /gene="obgE"
                     /locus_tag="PKM49_03095"
     CDS             complement(11281..12663)
                     /gene="obgE"
                     /locus_tag="PKM49_03095"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNY81835.1"
                     /translation="MEQTFVDRVKITVRGGKGGDGIVSFRRERGIPFGGPNGGNGGNG
                     GNVFLVASPALRTLLPFKFQQHFFASNGEPGRSSDQTGASGKDTVLEVPLGTVVFDSE
                     TEELLSDLTAKGQYVCVARGGRGGRGNSVFSSSTFQAPKIAEKGFLGEERKLLLEIKL
                     LADVGLVGFPNVGKSTLITKISNSRAKIGDFPFTTLVPNLGVLKSPSGDGIIIADIPG
                     LIEGSHKGAGLGHYFLRHVERTTLFLHLLDVSLSERSDPIADYETIWKELIAYNPLLK
                     EKEEIVALNKADLSIPEVIEEAKAHFQRLGKTVFVISALTGEGIDELVKEIVRKVQAT
                     RAASQEGSDSFSFTESEPNKIPKVAPLWQPIPDRISLEIEKLAPDRYLVTGKALDDLS
                     RRLDLRYPDSFKKFMDIIENSRLNALLIKRGVQNGDTVVLMDQEYSFFDYSLCEEEEH
                     EESDDQITDV"
     gene            complement(12665..14044)
                     /locus_tag="PKM49_03100"
     CDS             complement(12665..14044)
                     /locus_tag="PKM49_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNY81836.1"
                     /translation="MLKFDFSHCVDSRFPIFNTREQLAARYRETEEALTTLKSQEPGF
                     LKILEHPEALDELLEYKPALSDFDNFVVLGIGGSALGNTALHRSLKPFQWEFFSREER
                     NGLCRVFVWDNVDPDFLHDQLWLVGLNPPSAEASKTVFNVISKSGNTAESMALYLYVR
                     KALEKQGLDPRKHIVFTTDPEKGILRKISRKEGFKAFSIPPEVGGRFSVLTAVGLLSA
                     LAAGIDIEKLFQGAREAKKRLLSTPVAENPAALIALHHLLYYEKGISLSVMMAYSNAL
                     YTWADWYRQLWAESLGKRVNRSGQEVFVGPTPIKALGVTDQHSQVQLYNEGKLDKVLT
                     FLEVESFRHRLDIPSMHPEHEGLSYLGNQKFNDLINLELRGTEYALTKHGRPSMRVVF
                     PRIDETHIGEFIVTYEMATTLMAALLDIDPFDQPGVELGKQATYALMGRAGYDALAKE
                     IRFAEGEDS"
     gene            complement(14096..14608)
                     /locus_tag="PKM49_03105"
     CDS             complement(14096..14608)
                     /locus_tag="PKM49_03105"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNY81837.1"
                     /translation="MDLYRFIRDIPDFPQKGVIFKDITPLLKDPEAFRETINRLIERL
                     EGMDFDYIVVPEARGFIFGAPIAYEMRKGFIPVRKPGKLPYRTRKAGYTLEYGQAELE
                     IHEDALHPGAKVVLLDDVLATGGTIKAIAGLIEELGGSVAKMLFLIELKFLGARKALE
                     AYSVDSLMSY"
     gene            complement(14664..16436)
                     /locus_tag="PKM49_03110"
     CDS             complement(14664..16436)
                     /locus_tag="PKM49_03110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNY81838.1"
                     /translation="MSETNNAKRNFFPIILFSICVVLFLFFQVHQIVIQEIISSKTPI
                     WWLMVLLAFSYFADNLRLKHKGMYITTIFLPLLTLMLWTGPYTACFFTAFLSLFRLNQ
                     VKLPFFAGANRYLFRFSVHFLMFFVPIIVFQVSIPFFLQLALYIVLAEITNVLLINIV
                     TPVIEPGKKKISKEVLSLFLIETALQLMVIPYLSLMKALITYEFSYAKAFFYMIPLVH
                     LLYYGMIRFHAGYTAGLEEHRKLDRMRVGLENMLGIVRTIRTTDDRVSILKNALTLFA
                     QTMGYEKAIISLINFEESKINRIASYGIDDADFKAISRMNALRPFYDIAFQDRFLFGE
                     TYFIPEEADPFSGAESPIYVFPGQKIWIKKKNLPLWKPKDLFIVPFFNEKQEVIGYVS
                     LDNPISGERPTIEDAQLAKIFSDQISRIVEASNEYQIVLEKSKRDMMTGLYNHSTFFD
                     ILQEKIREADFKHPLSLILFDIDDFKKYNDAYGHQAGDKVIIRVAEIMKQSVPYNAYL
                     SRYGGEEFALLLPETGKLAAIRQGDLILSRISSEVIHEKPITLSAGVATAPEDGFQPS
                     TIVSSADNSLYRAKRTGKNRIIAS"
BASE COUNT         3861 a         4374 c         4441 g         3983 t
ORIGIN      
        1 gtgctcgaat acctgagagc caatccctct atgttatata aatcccttac ccctaacctg
       61 accctcatgc ctgccgtttg ggaaagagcg agcaagcttc tttactcatt cagcaatatg
      121 caggagataa aaaccaaagg ccttatccct ctccaattgg tggtgggggt ctccgttgga
      181 cacgaaatga tggagatgct cttcaaatcc gactgcccca caatttctcc tatggatgtc
      241 attcgagagt actcgaaaaa tgaaaagttg cgtttactcg ttctcgaaaa ggcagaggca
      301 ggccatatgt gcgagctggc tcgattggct gatgccgtat cgattttgat ggaaaagtgg
      361 gatatgaaca ttgtcctttt tgccttggat ttgcccaatg atacctgtca aatgctgatc
      421 agcagacttc gttcatcaga ggtggatatc tctgacccta agatggaaga ggctttggca
      481 aaactctata tcaaaattgg cgattttgga ttggagactg ctatctgatg gatcgatcga
      541 atccccctcc ctatcccact atggcggatc tggaatccgc cttacaggaa ggcattcagt
      601 ccggttgttt attcagctac atcctttctt ctctcaaccg taggatcgac gaaacggcgg
      661 aagccatagc ttacgtttct ttgtctccaa atcgaaaggc gtggtctctg gtggtcaatc
      721 ccatcctctt ctctcaaata tacgaaacgc gatcgaaaga gcctcgggag agagttttaa
      781 gggccattct cattcacgag ttcctacatc ttgcgcacga tcatttcgac cgtggcttaa
      841 cccttctcaa atcgtactcg ttagagacac tcaacatcgc ggccgacctg gccgtgaatg
      901 gctgcagaaa acacggggaa ctgagcgtat tggaagaaat agggggcttg ttccctgatc
      961 ggcccccttt cgatttccct gtgagactgt cgatggagca gtacctggca ttgttgccga
     1021 aaacgaaggg ctgtatgctg gtttcggtcc caaaatggct ctcaggtttc tctcagacga
     1081 tggaagagct tcaaggtctt tcggataacg gaacccctta tgaagggagc gtttatgcgt
     1141 ccattagaga tttggcttcg tcagcaaccc tagaactgaa aaagcgaatc ggagagctca
     1201 tgcagggtct acagaaaaag ccttcgagtg acctgcgcag ccgaggattc ggcaggggcg
     1261 aagacgtttg ggtgaccgat tgggtattcc cgcgtgtgaa gaacacatgg atgcggcacc
     1321 tgagaaacgt cttgacggga tcggggtctg gcgttgcaaa cacccgttcc tatcgacgcc
     1381 ctcatcgacg ggaaggaggg ggcattcttt tgaaagtaaa tatctcttca caaaaggagc
     1441 agaccctgat catactcgat acctcgggtt ctatgatggc tgcggactac gaactgttta
     1501 tgggtttgat caaaacgttg aatagcgacg ctcgcagctt ctacgtcatg cagtgggact
     1561 acgagcaaca gacggagccc gttcccgtca ccgtatttat ggccaagaag aagcccgagt
     1621 tccacggcag gggcggaaca gatatgcaac aggccgtgtt ggtagcctca caacgctatc
     1681 cgcagtataa aaaaatggtt gtggtgacgg atgggggcac accgtacttc accaaagatg
     1741 atccgtcccc ggtgccggta atctgggtac tcacccataa tgcgccgttt aacaatgccg
     1801 atggggttgt ggtccgttta cgcgaggatc gagatacgta aacgcgccga aggggagaga
     1861 aaaggagatg aagaaggaag agacgtactg gatcccaggg ttggaccaaa atgaacacaa
     1921 agtgctctct ctgtccgata tagggctcat cttgagaggt tccttgcact cggcgaaaaa
     1981 cgccaatgcg atgtctgtga agggcaggct gtcagccgcg ttgaacgcgc tctcgattac
     2041 ttccgagatg gtgcgcgtgc gggcgggtag tttcaaaatg ggccatacac gtgaagaccg
     2101 agtagaggat gccatccact taggattctt ttcccatctc gtgaccctca cctacgacta
     2161 ttggataggt cgttatcccg ttacatgcca ggaatacgat tgtttctgcg agagcatcgg
     2221 aaaagcaaag ccacgtgatc ccttcgaaga gaacgaatgg gttcctagaa gatattttcc
     2281 tgtcggctac ttgaaatggg gcgacgccgt caggtactgc aactggctga gcctgcggga
     2341 gggtctgaaa cccgcctttg attttgatgg gaacttgttg gacacgaatg gaaacataac
     2401 gaccgaaata aaaaatgtcg agggctaccg cctgcccacg gaagcggaat gggaatacgc
     2461 cgcccgcggc gggcataaga gaacgagaga ttaccggttc cccggaactg acgacttgga
     2521 ggatttattg atcccctatt atgacgacaa acccttgggg caaaccaagc ccaacgattt
     2581 gggcctgttc gatatgagtg gaacgatttg ggaatggtgt tacgataggt attttgacta
     2641 taccaactcg actgagaggg tcgaccccat aggtcctacg aaagggaggg cacgggttat
     2701 aagaggctgc gggctagatg taaactacgc cttagacaga ggccgtgaga ttgaatttaa
     2761 cgttgcctac cgctacgaat cccctccaat tagcaggcgt agtgggttgc gcaccggttt
     2821 tcgcgtggcg agaacgtgcg attgggcgag aggagacggg caagaacctg ccgaacggaa
     2881 gtttgtcatt cccagaaaac agcgttcaca caaagggcac agaggccacg gagtaaaaaa
     2941 agaaataccc ctggagttct aagaaacagt aaaacatgcc aacttgaaag ttgaccccac
     3001 gaagagaagg ggcgcttgcg ccgcgattag cgcgcgggtc atcgcggggt aaccaatggg
     3061 accccgcgat acgccttttt tctttacacc gcgatgatgt cggggtatcg cgccgtcgcg
     3121 agaagaagga gcgcttacgc cgcgatgccc gtgttggggg tatcgcgccg tcgcgaaaag
     3181 aaaaagcgct tacgccgcga tgcccgtgtt gggggtatcg cgccgtcgcg agaagaaaaa
     3241 gcgctacgcc gcgatgcccg tatggggtca tcgcagggta accagcgggc tgacttcgaa
     3301 ggcccacgaa ctctcgcgat acgctctatg tctttctaaa aagggcatca caccgtcgcg
     3361 aaaagaagga gcgcttacgc cgcgatgccc gtgttggggg tatcgcgccg tcgcgagaag
     3421 aaggagcgct tacgccgcga tgccccacta gggaatgctc ccagtgaaaa gataaagcca
     3481 tgccaaacta aagtttggcg ctcccagaag acatgccgga ctaaagtccg gcgttcccag
     3541 gaattcccag gcgttccact ttcggtgttt ttcgtagcgc ccggtaaatg gtataattca
     3601 tcggttattt gatgggggtg ttctatgaat gtgaactgtc tttccgagat cacggaagcc
     3661 tatctccaac agaaggtctt tcccgggggg gttatcggct tcggaaacgc ggatcggttt
     3721 tcggtttttc cttttgggaa gctcgatgag gagcagccgg tctgtgtcga ggcgttgtat
     3781 gatgtcgcga gtttgaccaa ggtcgtcgcc acgttgcccg ctgtttttac gttggttcaa
     3841 aaaggcaaga tagacttgga agatcccgtt tcccggttct tgcccgttcc ttatccttct
     3901 atccgcgtgt ggcaccttct gacacacagc tccggctttc cgccttattc cgacgcctac
     3961 aagtgggcgg ccaaccccga ggagatcctg caggagatct accgaaaccc gtgggtgaag
     4021 gagccgggga aagaggtggt gtattcctgc ctcaatttca ttttgctcaa acagatcgtc
     4081 gcgtccatcg tcggggacta cgcggcttac ctcaaagagg ccgtgtttgc gcccttgggg
     4141 atgaacaaga ccggcttttt accggataat caagaaccca tcgcgcccac ctcttttcga
     4201 gacggcgtac ggttgcgagg cctcgtcgac gacgagctcg cctactattt gggcggagtc
     4261 agcgggaatg ccggcctttt cagctgtata gaagacctgt gtcgatatgc gcaagaatgg
     4321 atccgcccac gccacatcct cgtaccctcc actgtcgcgt tggcgacgcg cccgtggact
     4381 tcccaaattc ccggtgatcc caaagggtta ggttgggcgt tgcatacctc gaggacctcc
     4441 gacggttcgt tgttttctcc gaatagtttc gggcacaccg ggtttacggg aacgagtctg
     4501 tggatagatc ccgaccacgg ccgcttcgtc tgcattctga ccaatcgctg cttctacgat
     4561 cggcacacgg cgcaaagcaa agacgccatt tgggagtttc gacggcgtgt cgcgcaagtc
     4621 gccgtcagca ttatggaaag gggtgaggtc gtatgaagcg gatcatcggt ttgatgtccg
     4681 ggacctcgtg cgacggcttg gatatcgctt gttgccagtt tgtcaacaat gcggagcgca
     4741 cggagctcca gcttgagcac ctcgaaagta tcgaataccc ccccgaccta cgagattgga
     4801 tccgtggcct gacttcggga aagatctccg tggccgaggt cgcgcaagcc cattttcggt
     4861 tgggtacgct gtttggcgat tgggcgttcg atcatattcg tcgccatcag ctcgagggga
     4921 aagtggactt gatcgtcagc catgggcaga cggtcttcca tcgcccattt atggggcggt
     4981 cggaaaacga accggcctgt acgcttcaga tcggtgacgg agatgtcatc gcctctataa
     5041 ccgggacgcg ggttctttcc gatgtccggg tgaaggatat ggcttacggc gggcaagggg
     5101 cgcctatggt cgtttgggcc gactacgttc tcttccgatc gaaagaaaaa aacgtggcga
     5161 tgcaaaacat cggaggaatc ggcaatgtga ccttccttcc caaagccggc ggagtagaaa
     5221 gcgttttggc tttcgatacg gggccgggca acgtcctcat tgacgaggcg tgccgtaccc
     5281 tattcgagcg taagttcgac gaagatggcg agatttcggc gaaaggtact gtgaaccgtc
     5341 cgttgtttaa taggatgcgc gaaatagaag aaggctacgt ggcagagcgc ccgccgaaat
     5401 cgaccgggaa ggaaatacgg tatcaccagc agtatagaga acgcctttgg aagctgagag
     5461 aagaattcgg ctgttcccca cacgatttcg tgagaactct ggtggcgttg acggcgtggt
     5521 cgatcggcga gagctaccgt cggtacttgg gggaagtcga acgggtgatc ttgaccggtg
     5581 gtggcgcgct taaccccact ctcgtcgccg aaatcatcgc ggagctcccg ggaacggaga
     5641 ttcgtaaact ggacgatgac tggacggcgt ttaaagaagc gatcgccttc gccgtaatcg
     5701 ggaacgaata cctcaatggg cacccagcga acgtcccttc ggcgacgggg gcccgaaaag
     5761 ccgtcacatt aggaaaattg agcttgcccg aatgagtcac caatttaaat tagaattagg
     5821 agtctgagta gatgaatatc gctatgttga cggataccta tctaccgcac gttaacgggg
     5881 tatccacgag tcttttcctg tttcgtaaac acctggaaag gcttggtcat cgtgtttata
     5941 tcgtttctcc cgttggtcaa aaaggggatc cctatttttt caaagtgatg ggttttcggt
     6001 cgatgcaagt caaagaccaa aggatcgctt tgcctaacac caaacgcatc ttggattttc
     6061 tggaaacgaa tcagatcacc cacctgcaca gccacgctcc cctgtcgatg ggatggacag
     6121 ctttgtggct ccagaagcgc tacggattca tccatttcca tacctaccat acgctgttgg
     6181 ttgactatct gcactatatc ccacggtttt tcaggccgac gaaaaaggtg atccaggagt
     6241 tttcaaaatg gttttgttcg gaagtggatt gcacgatcgc gcccaccaca gagatgaaaa
     6301 acgagctgat ggcctatggg atgaaaaagc ccatccacgt catacccacg gggatcgaca
     6361 ccgatttgtt tcgaacggcc gcgcctatcg acctgcgcgc gaaacaccac ttgccgaagg
     6421 agtctttcat cgcgttgttc gcgggacgca tcgcaaaaga gaaaaatatc gtctttcttt
     6481 tggaagtcgt cgaaagaatg attcgaaaag gtcattcctt ctatttagta ctggttggaa
     6541 acggtccgtt ctttgaggag ctctcggaga ttgtccgaaa aaagcggttg tccgagaacg
     6601 tgatattcgc cggagagatg aagcgcgagg agttggcctc ttattatcga tctgccgact
     6661 gtttcacgtt cgcttcgaca acggaaacgc aggggctcgt cgtcttagag gctttagcgg
     6721 cgggtttacc cgtcatcgcc gtcgcgcaaa aaggcgtgaa aagctcgctt tcggaggggg
     6781 aaggatgttt tctaacggaa catcccaatg tggatttctt cgtggaaaag gcgcttctct
     6841 tgatgaaaga ccccgagcta cgacaacaga tgcgtggcaa agggatggag tacgtccaaa
     6901 aacattggag catagatgtc tttgcccaac acgtgtcgga cctttacggt caatttcaaa
     6961 aacccgagca atacaccccc caaaagatgt tcgagttttc cgagctttgg atgtgggtca
     7021 caggaaaatt gcgcaacatc caggacaaca tcttcaaatg agggggctgt ttgtatgacg
     7081 cggatgcccc gaaacgtccc aagcttttta ccgctcctcg tcgttttgac cgggctgttt
     7141 tccgttctgg cgttttctct cgtgttgtat ctggagacgg attcttttct ggatatcatt
     7201 gcacaaaccc tgtccgcgct gtctttctat ttgcaggtcg cgctgtttct ggtatgggcg
     7261 ttgcttctga atcagaacat caagctgacg taccttcgag cggaagagtt cattccccaa
     7321 gccgatgaag aaacgagttt cgacctccgc tggttgcaaa ccaatttacg aaaaaacacc
     7381 gtcccgctgt ggccactcgc cctcctcacg gtctttaaca ccctttcgct gctgttcgac
     7441 ttcgacctgt acctgttgtc gttagccagt gtgtccctta gccttttctt cctctatcgg
     7501 ctcctgttct gcagtcgcca actgttgacg ttgaaatcga atttttatag tttctacaag
     7561 gtagaaggct acactgacca gttccaatcc atctttccaa accgcacgct cttatccttc
     7621 accttgctgg tctttctgac cctcggattt tatctcttct atttcttctt gcgtttttca
     7681 gcggaactta acctctattt ggccttagac ggggggtatt tggaccgcct tgcgcttccc
     7741 gaaaaaccgg gcgattgagc ctttcctctg aagatttcac actgggactc gatggggttt
     7801 ggataaaccc gagcgttcag cagcccgtcc aaagagcgac tgccagtctc cacgcctttt
     7861 tttcgtcttc gcaggaggtt tcgttgcaat accgataatc gaacttccgt tcgatctcgc
     7921 ggaggtggcg cgccaactga tcgtattttc taatttgcgc ggatagaatt ccgtttgggg
     7981 aagcatccgg tttgcttttt gaaaagacgg cctccagatg ggggacgcag aggtgcccgc
     8041 gctcgtcaaa ggcctttcga aaggtttcgt cgcagtcgta aaacatcgga atcaacggta
     8101 gaacccggtc ttctaactcc cgtaagccct cgcagataag gcactcggac ccgttcgggg
     8161 cccgtaagcc ctcacgggcg ccgaagcgtt ttcgcccatc cgaggaggac cggcgactgg
     8221 cgatgtcttc acctgagcgg agggtctcca ctttttgttc aaataggcga ctgtagatga
     8281 tggcgtgcaa cagcgggtct ccttgctcta gtatccgcag cgcgtgctca cgacaaaacc
     8341 cgccggattg gtgtatcttc cggcggattt cgtagtcgtc gatcctctcg taccgtaagg
     8401 agtcgatgat ccgttccagc tgccggtccg ccaaacgaca gaccacacaa ccgtccgcgt
     8461 tcatcgcttc cagcaacgag aagtaattgg gatcttgtcc tacggtttta tggtttccaa
     8521 aacgcacggt tcattcccat tcaatcgtgg cgggcggttt cgcggtgatg tcgtaaacga
     8581 cccgaccgac ctccgccacg cggttaataa tccgcctggc cgcctcgtcg aggatatgat
     8641 aggggatgcg cgaccaatcg gccgtcatcg catcgctact atccaccgaa cgcagggcga
     8701 cgacgtttcc gtacgccctt tggtcgccgg ctacccccac gcttttcaag ggcaggagaa
     8761 cagcgaaggc ctgccacacc ttttctttcc aaccgaactc gtgtagcgtc tggaggaaga
     8821 ttgcgtctac ctctttcagg attcggaccc gatctgaggt cacctcgccg aggcaacgga
     8881 tcgccattcc gggacccgga aaggggtggc gggccagtat ctgctcggga atattcagga
     8941 tcgctccggc tcgtctcact tcgtctttaa ataagaagcg caacggttcg actatcttga
     9001 agcgcatatc ttgcggcagc ccgccgacat tgtgatgcgt tttgatcttg atggtgccac
     9061 cgcccagcgt accggcgctt tcgatcacgt cggaatagat cgttccttgt attaaaaatg
     9121 gaggtgaccc gatctcgcga gcctccgatt cgaaaacgcg tatgaaggtc tcgccgatga
     9181 tcttgcgctt ttcttcaggg tcgaccactc cggccaagcg ggcgagaaaa gtctccctgg
     9241 cgttgaccac tcgcaagtta aggccgaaag aacgccttaa caaatctggc acgacggcat
     9301 cctcgttttt tctcaaaagg ccggtgtcta cgaagacgcc ggtcagctgc gacccgatcg
     9361 cttggtgcgc caaaacggcc gcgacggtgg agtctacgcc tccggaaacc gcgcaaaccg
     9421 cgcgttgtgt tcctatcgta gcccgcaggt tctctctttc gattgcgatg aaatccttta
     9481 gtgtccaatc gggcgtacac ctgcagattt tgaaaaggaa attcgaaaaa atgcgatccc
     9541 cgaactccgt atggttcact tccgggtgga attgcaaccc gtagatcggg tctttcacgt
     9601 ggaaggccga ggcgataatt tcgttctcgc tgagggcgca gatggcgaac ccggggggaa
     9661 gctccaaaac ggagtctccg tggctcatcc acacggtgag cgaccggtcg atcccgtcga
     9721 acaaggggtt ttctccttgt cttacgatag gcatacggcc gtactcggag gagtgcgcgc
     9781 gatcgacacg accgccgaag tattgggtta acagttgaaa tccatagcag atgccgagaa
     9841 tcggcacctc tttctctatg aggggcttga cccaatcggg gattctcggg gcccgcgggt
     9901 cgtatacgct gaatggcccg ccggagagta tgagccccga aatatcctcc aaattcggtt
     9961 gggaagtagc ggtgaccacc tcggagaaaa cgcggagatc ccgggtcttc ctggcgatga
    10021 gttgggtgta ctgtgaaccg tagtcgacga tgacgatttt gcccacgtta gagactcttt
    10081 ttctgcaagt ggaagtaatc tcgaacctca tcgggcgtgg ctattggccg gccgagctct
    10141 ttcgctattc gaacgattct tgcgaccaat tccgcgttgg attttgcgag cacgcctttt
    10201 gagtaataca ggttgtcttc catgcccacc cggacgtgcc cgcccatagc gagcgctacc
    10261 gtggccatag gaagttccat tctgccgatg ccggcgacgg tccaagaggc ctcagccggg
    10321 atctcctccc gcatacgcac caacaaaggc accgtcgcgg ctatcgctcc cgggacaccc
    10381 atgacgaagt catagtgaaa gtgcggccat acgacgtggc ctttttggac cagccaattg
    10441 gcaaagtgga tgtgtccgat ttcgaagcat tcaaactcgg gcataatcgc tcgttcactc
    10501 attttttttg cgaattccag caccatgccc ttcgagttga gaaagagatc gtcgccgaag
    10561 ttgcaggatc cggtcgagag cgtggccata tccggcgctg actcgatgct ttgtatgcgt
    10621 tcttctgctt tcatccaagt ggcgccaccg gttgagacct gcacgatcag atttggcgct
    10681 ttttgtcgaa tcaaacccac cgttcgcttg aatatcgcgg cgtcttgggt agggttgccc
    10741 tccgggtccc gaacgtgaag atgaacgata caggctcccg cgtcataaca tttttttgct
    10801 tcatcggata actcttccgg cgtgaggggg aggtacggag tctcgtttct ggtcacttcc
    10861 gccccgcaaa tggccgccgt gatgatcaat tggtcgaatt ttgccatatg atctcctcct
    10921 tacatatctc gatatcgttg tttgctgacg ggcaccacgc acgtcccgct ggctttgcac
    10981 actaagattg gctcatccag aaccacgcac gcgctcggct gttccgggat agacgcgttc
    11041 gtgatcacct tgcgcgcttg gaacgtcatc ttccgagagg attttcccgt ctccacaatc
    11101 tccccggtga tctccaggta gtctccggcg tacaccggcg ctaagaattc gatcgaatcg
    11161 taggcgcgaa aaagcccttc gtctccgtcc aatacgatca acagttccgt cgcgacgtct
    11221 ccgaacaact gtaaaatctt cgccccgtcc acaagtccac ccgcgtagtg ggcgtcggcc
    11281 tcagacatcc gtaatctgat catcgctttc ttcatgctct tcctcctcac ataaagaata
    11341 atcgaaaaac gagtattcct gatccattaa aaccacagtg tctccatttt gaactccgcg
    11401 tttgattaat aaggcgttga gccggctgtt ctcgatgatg tccataaact ttttaaaact
    11461 atcggggtaa cgcagatcca agcgccggga cagatcatct aacgcctttc ctgtgacgag
    11521 ataccggtca ggcgccagct tctcgatttc taacgaaatc ctgtccggta taggctgcca
    11581 cagcggggca accttcggga tcttgttcgg ttcgctttct gtaaacgaga acgagtccga
    11641 tccctcttgg ctagcggccc gcgtcgcctg aaccttccgc acgatctcct tgacgagttc
    11701 gtcgatcccc tcgcccgtga gcgcgctaat gacaaagacc gttttcccga ggcgttggaa
    11761 atgagctttc gcttcttcga taacctccgg gatcgaaaga tcggctttgt tcaacgccac
    11821 gatttcttcc ttctccttta aaagagggtt gtatgcgatg agctctttcc agatggtctc
    11881 gtaatcagcg atcgggtccg atcgctcgct caaggaaaca tccaagaggt gtagaaacag
    11941 cgttgttcgc tccacatgcc taaggaaata atggcctaaa ccggccccct tatgggagcc
    12001 ttcgatcaac cccggaatgt ctgcgatgat gatcccgtct cccgaagggc tcttgagaac
    12061 gcccaagttc ggcaccaacg tggtgaacgg gaaatcgccg atcttcgcgc gagagttgct
    12121 gatcttcgta atcaacgtgg attttcccac gttcggaaat ccgacgagcc cgacatccgc
    12181 cagtagcttg atctcgagca ggagctttcg ctcttcgccc agaaagccct tttctgcgat
    12241 cttcggcgct tgaaaggtag agctcgagaa aacgctgttt cccctacccc ctctaccccc
    12301 tctggcgacg caaacgtact ggcccttggc cgtgagatcg gagagtagct cttcggtctc
    12361 gctatcgaac acgacggttc ccaaaggaac ttctagcacg gtgtctttgc cggaggcccc
    12421 ggtttgatcg gaagaccttc ccggctctcc gttggacgcg aagaagtgct gttggaactt
    12481 gaacggcagt agggtgcgca atgcggggct cgccactaaa aagacgtttc cgccgtttcc
    12541 gccattaccg ccgttcgggc ctccgaaggg aatgccccgc tcgcggcgga aactcacgat
    12601 cccgtcgccc cctttacctc ctcggacggt tatcttaacg cggtcaacga aagtctgttc
    12661 caaattagga gtcttctcct tccgcaaacc gtatttcttt ggcaagagcg tcataacccg
    12721 cgcgacccat caacgcgtag gtggcttgtt tcccgagttc caccccgggt tgatcgaagg
    12781 ggtcgatatc gagaagggcc gccatcagcg tcgtcgccat ctcataggtg acaatgaatt
    12841 cgccgatatg cgtttcgtct atgcggggga agaccacgcg catcgagggg cggccgtgct
    12901 tcgtcaacgc gtactccgtt ccccttaact ccagattgat caaatcgttg aacttctgat
    12961 tcccaaggta agagagtcct tcgtgttcgg gatgcataga cggtatgtcg agcctgtgac
    13021 gaaagctttc tacctcgaga aaggtgagca ccttgtccaa cttcccctcg ttgtacagct
    13081 gtacctgcga atgctggtct gtgactccta gggctttaat aggcgtgggg ccgacgaaaa
    13141 cctcttggcc ggagcgattc acccgtttgc cgaggctctc cgcccacagc tggcgatacc
    13201 aatccgccca agtgtagagc gcgttggaat acgccatcat cacggatagg gaaatgcctt
    13261 tctcgtaata gaggaggtga tggagcgcga tgagcgccgc cgggttctca gcgacaggag
    13321 tggagagcaa acgcttcttc gcctcgcggg cgccttgaaa gagcttctct atatcgatgc
    13381 cggctgcaag agcggaaagg agcccgacgg cggtgagaac gctgaagcga cctcctacct
    13441 ccggcggaat agagaaggcc ttgaatccct cttttcggga gatttttcgt aggataccct
    13501 tttccgggtc agtcgtgaag acgatatgtt ttctcgggtc aaggccctgt ttctccaagg
    13561 cctttcggac gtacaggtat agggccatcg attctgcggt gttgcccgat ttcgagatca
    13621 cgttgaaaac ggtcttggag gcttctgcgc tcggagggtt caagccgact aaccagagct
    13681 ggtcgtggag aaagtccggg tccacgttat cccaaacgaa gacgcgacac agaccgttcc
    13741 gctcttcgcg agaaaaaaat tcccattgaa acggttttaa tgagcggtgt aaggcggtat
    13801 tccccaacgc ggaaccgccg attcccaaca cgacgaagtt atcgaaatcc gaaagcgcgg
    13861 gtttgtactc gagcaactcg tcgagcgctt ccggatgttc caggattttt aagaagccgg
    13921 gttcctggct ttttaacgtt gtgagcgctt cctccgtttc ccgataccga gcggccaatt
    13981 gctcccgggt attaaagatc gggaaacgag agtcgacgca gtgagaaaaa tcgaatttta
    14041 gcataagttc ctccagtgct gtattctttg acttatcgtg cgggggcctt cgtcctcaat
    14101 aactcatcaa agagtcgacc gaataggcct caagggcttt acgggcccct agaaacttca
    14161 gctcgatgag aaagagcatc ttagccacac tcccgcccaa ctcctctatt aggccggcga
    14221 tggctttgat tgtgccacct gtggccagga catcgtcgag caagacgact ttcgcgccgg
    14281 ggtgaagagc gtcctcgtgg atttcgagtt ccgcttgccc gtactccagc gtgtacccgg
    14341 cctttcgtgt ccgataggga agttttccag gctttctgac gggaataaag ccttttcgca
    14401 tttcataagc gatcggggct ccaaagatga accctctcgc ttcaggtacg acgatataat
    14461 cgaaatccat cccctcgagg cgttcgatta aacggttgat ggtttctcgg aaagcttcgg
    14521 ggtctttcag taacggtgtg atatccttaa aaataacacc tttttgaggg aaatcgggga
    14581 tatccctaat aaaacggtat aggtccatcg tcatttctcc ttatcagcgt tgtcacaaga
    14641 ttataccata accccaatca gtatcaagaa gcgataattc gatttttccc agtcctttta
    14701 gccctataca aagaattatc cgcggacgac acgatggtgg aaggttggaa cccgtcctcg
    14761 ggcgcggtcg ctactcctgc gctcaacgtg atcggctttt catggatcac ttccgaactg
    14821 attcgtgaaa ggatcaggtc tccctgtcgg atggccgcca actttcccgt ttccggaagc
    14881 aagagagcga attcttcacc cccgtaacgc gagaggtagg cattataggg aacggattgc
    14941 ttcataatct ccgccactcg gatgatcacc ttatcgccgg cttggtgacc gtaggcgtca
    15001 ttgtactttt taaagtcgtc aatatcgaaa aggatcaagg acaaagggtg tttaaagtcc
    15061 gcttccctta tcttttcttg caagatatca aaaaaggtcg aatggttgta cagcccggtc
    15121 atcatatccc tcttcgattt ctcgagaacg atctgatact cgttggaagc ttccacgatt
    15181 cggctgatct ggtcgctgaa gatcttcgcc aattgcgcgt cttctattgt gggccgctcc
    15241 ccgctgatcg ggttgtccag ggacacgtac ccgatcacct cttgcttttc attgaagaat
    15301 gggacgatga aaaggtcttt gggtttccat agcggcagat tctttttctt gatccagatc
    15361 ttttgacccg gaaagacgta tatcggagat tccgcgccag agaaagggtc cgcctcttcc
    15421 ggaataaaat aggtctcccc aaacaaaaag cgatcttgga acgcgatatc gtaaaacggg
    15481 cgtagagcgt tcatccggct tatagccttg aagtccgcgt catcgatgcc gtatgaggcg
    15541 atccggttta tcttggactc ttcgaagttt atcaacgaaa tgatcgcttt ttcgtacccc
    15601 atcgtttgcg cgaaaagtgt cagagcattt ttcaagatag agacgcggtc atcggtcgtg
    15661 cggatcgtcc gcacaatgcc gagcatattc tctagtccaa cgcgcatacg gtcgagcttc
    15721 ctatgttcct ccaaaccggc cgtgtatccg gcgtgaaacc ttatcatacc gtaatagagt
    15781 aagtgaacca atgggatcat atagaagaag gccttcgcgt aagaaaactc gtaggtgatg
    15841 agggccttca tcaaactcaa ataaggaatg accataagct ggagtgcggt ctctatcagg
    15901 aagagggaga ggacctcctt cgagatcttt ttcttaccgg gctcgataac cggtgtgacg
    15961 atattgatca acagcacgtt ggtgatttcg gcgaggacga tataaagggc taattgtaaa
    16021 aagaacggaa tagagacctg gaaaacgatg atgggaacga aaaacataag aaagtgcacc
    16081 gaaaaacgga atagataccg gttagctccg gcgaaaaacg gaagtttgac ctggttcagc
    16141 cggaatagtg aaagaaatgc ggtgaaaaaa cacgcggtgt aagggcccgt ccacaacatc
    16201 aacgtcaaca gcgggaggaa tatggtcgtg atgtacatcc ctttgtgctt caatctgaga
    16261 ttatccgcga aataagagaa agcgaggaga accatcaacc accaaatcgg ggttttcgag
    16321 gagatgatct cttggatgac gatctggtgt acctggaaaa agaggaagag aaccacacag
    16381 atcgaaaaaa gaatgatcgg aaagaagttt ctttttgcgt tatttgtttc ggacatcacg
    16441 catcccccct tttctcctcg actgctcagc gccaaacggc aaacctatgc tgtgactgtt
    16501 ctcgcctcgc ctttctttac gaaaagcatc gccgcgaagg ctaagacgaa acctcccata
    16561 gaaaagaaaa tcatagattg ataatcaaaa tggtcgatca atagccccac gaatggtggt
    16621 gccgccaccg ccgccaacat cgagaagaag tagtataac
//
LOCUS       DAOPXN010000033        16582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3538_length_16582_cov_5.170630, whole genome shotgun sequence.
ACCESSION   DAOPXN010000033 DAOPXN010000000
VERSION     DAOPXN010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16582
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3538_length_16582_cov_5.170630"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKM49_03115"
     CDS             <1..252
                     /locus_tag="PKM49_03115"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HNY81839.1"
                     /translation="RTPRTVVAIAPNKLVFIVIDGYQSSSAGLTIEELASFLISKGFT
                     DAMCLDGGSSSTMVVKSRVVNNPPGGEPEIPVGLLVKPK"
     gene            316..2379
                     /gene="fusA"
                     /locus_tag="PKM49_03120"
     CDS             316..2379
                     /gene="fusA"
                     /locus_tag="PKM49_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNY81840.1"
                     /translation="MEKRKTVTLIGHNGSGKSVLAEAFLVKTGKAEKIGGIMNYDPLE
                     IERGTSLSNAIATFEYGDTQLTVIDTPGFGDFISEVILGVFVGENVLSIVNAGAGVEV
                     QTERTWRIAQEQKKPIMVFINQMDKERADFDQTLDDLKKTFDIKMLILTLPMGSGESF
                     NGVIDVLTQKAYQYAADGSGKTAEIPIPDTMKARAKEVYEKTIEDIVETDEVLMEHYL
                     GGEEIELDQLAAALKKAYCSGQVVPVLAGSATKNAGTDRLLDWLTRLCASVNEGHPIT
                     TTMVEGGAEVEVLPSENEPFVGLIFKAAVDPFIGKQSFIKLYAGKIATGDGFVNTTKG
                     SYEKAARIYTIVGKEQKEISEAGAGDIVSIPKLKESSVGDSLSLAARKLLIPTIKFPE
                     PMLSKSVKPISKQDIDKISNGLARLSESDPTFVWEHDTETSETVISGMGAVHLDVMIE
                     RLKRLFKVEVEVGKPKIAYRETVKDFADAEYKHKKQTGGHGQYGHVKIKMSPLPRGTG
                     FEFIQTIFGGSVPYNYIPSVEKGVRSALKKGVLAGYPVVDVKVDLYDGSYHEVDSSDM
                     AFQIAAIQAFKKAMSMAKPTLLEPIMEVEVSVPDENAGDAMGEITSRRGRPLGMETGA
                     KGFQVVKAKAPFAEMLDFSNKLGSITSGKGHFTMKFSEYSEVPGNIQEKIVAERKREQ
                     EEANK"
     gene            2411..6217
                     /locus_tag="PKM49_03125"
     CDS             2411..6217
                     /locus_tag="PKM49_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNY81841.1"
                     /translation="MTCLKTLNRYIMKSAMFPFVLGLGGFIVFVSIELLYQLSEVIVS
                     YKVPIWRLFELLYYNVPMFTVMGVPVGVLLALFWILSQMGSNSELMALQVHGVSFKKI
                     VIPFLILGLIFSVFSYLLSDFVVPDFNHRASVTLTRYVFKQPESTIKANQFLDVGDNR
                     YFYVKRFDTEKATFEDVLLYDLSQSDMRIYYARQAIKSEDTGRWKLVEGRVFTMDRQG
                     LMKLDTVFQETEIDLTKDLDEFIRDTSKRAQDLTSKELWVKIEAYKKMGIRTNSLEVE
                     FHTRFANALGPIIVAFLGVPLSLTFNIKSKSWSVIFTFILIVLYQGSGAWMSALGKDG
                     ETLTPFLASWLPDIGFAIIGFILFILLDTKLMYRLKEMFSRLFTFSVTSRVFILLLFL
                     FFWQVTGFCNNQTTSKTGTDTTAVATPTAPPKAPKEPAPSLPTTNPLIPASDELLIKI
                     SADEMDYNGREKTITLTGNVIIEYDQMTLQAPRVTAYLENEEWVDYFLAESEGATKVT
                     LKQEKEYYQAQLVKAYVKRNAFFLYHLRGTFKQKNQKGQEKPLILVSDKSRSKTFVNK
                     HVTEVQTGYLTTCDLDKPHYRFEAGYIVVKESKEIIAYDLLLYVLNIPILPYPVYFAD
                     MTKEKQPVEISFTLSGEGGLQTHLEYTYLDRANEKGVIKFDTVEKGPKKGATIVTENK
                     FALPFDIIGYLNMTHKSTNDFKDQTRILDFNVSKTFENKLIMKAYYKSTRVLENEATS
                     KRDGSVGLEFSGPFFLKGLSFSLKANRTENRSLTASKNTYEFPTFTIGSYSQVLFKDF
                     YPVTITLDPFSLTGKAVTPAATSIVEALDVLDYTTHQSIKIGYSALKVDDVTLVKPMK
                     TDLVLDMDFNKRDFTNYFIIKNTVPFQNVGFQFGQKDKAFVGFDSAYTVRLGYIRHRN
                     NQTAWRYSENVLSEASVNLWWLNHQMTHNYVFLIADGENYSALGEDVAERNALSMTTI
                     LQVPFLFSKFTLKNGFDFMKKSDRMSDPTLISNTTIKVQQLSFDMTTTSPYSLELKQF
                     VSTVYTFNLTHPYGNSSLDFTYVYEQPDEKNKKKGEEWKGPVDKITNSNSLRFNNLGL
                     VSSFTEDLNFTWTTSGTATETGGFELNKLIQKNTLKLKGFGTLKSLQNTFDFEYFPQK
                     PAKDRWSYINDDLSLEFDKFTTTMTLKWKPRKSMYLGLVGTFPGFSFKFDTNYNIETF
                     AFQSMGLTLNKDLHCWENTTEIKFDYIDSKFKLASFATTFRIKSFTEKFFRADPVKKT
                     FDLSIF"
     gene            complement(6274..7107)
                     /locus_tag="PKM49_03130"
     CDS             complement(6274..7107)
                     /locus_tag="PKM49_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HNY81842.1"
                     /translation="MIEIPESVVLAKQISQSLRGKTVTKVLPQRTPHKMAWYYERPED
                     YPKLLTGKKIEFATGIGSMVEICASGAVILFGDGIRLTFCPTDSTVPKKDQLLIGFED
                     GSYLSAVVQMYGGLWCFPSGSFDNPYYLSAKSKPFALSDAFNQQYFEELIDNPDMQKM
                     PAKGFLATEQRIPGLGNGVLQDILFRAGLHPKRKIGTLTKEEKSLLYKILKTTLKEMA
                     EFGGRDTERDLFGACGGYQTQMSKNTAGKPCPRCGETIAKEAYMGGSVYYCPNCQKIP
                     A"
     gene            8192..8815
                     /locus_tag="PKM49_03135"
     CDS             8192..8815
                     /locus_tag="PKM49_03135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY81843.1"
                     /translation="MGNKSNTRLFNGIAPIYGLFYRRQKSEFRKVLERAHHVLDPRRF
                     KTVLDVGCGTGALCAALYEKGFDVTGIDPAENMLKIARKKARHTQIRFIQASVLEGLP
                     FADRAFDLSIASYVAHGLKPQDRQRLYAEMSRVTKSQVIVYDYNAKRGLLTTVIEWLE
                     RGDYFNFIRLAESEMKSCVFYLKECFSEVRVLEVGKRAAWYIGKPAR"
     gene            8981..9688
                     /locus_tag="PKM49_03140"
     CDS             8981..9688
                     /locus_tag="PKM49_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY81844.1"
                     /translation="MEKKYLNPLIDYTFKKVFGNEKHKEILIHFLNSLGIGSQGHKIA
                     DVRILDHTIEKRFRPNKNSIMDIKATFSNGEPVNIEVHILPYTHVAQRATYHLAKMYS
                     DQAEQSDDDDKLHNTVVVNVLGHNQFEHDKIHSVYRMLDSKTCEKLCDTLEIHFLEMP
                     KIGTSSQKLGKELTGWLLFLTDPGNQRTIELGKEVGEIGQAINLLEELSQSREERAIA
                     EEREKALNDERSFGNVE"
     gene            10252..11241
                     /gene="msrB"
                     /locus_tag="PKM49_03145"
     CDS             10252..11241
                     /gene="msrB"
                     /locus_tag="PKM49_03145"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNY81845.1"
                     /translation="MTSDGIREYLWKNAQKDKRILYLAGGCFWGVEGYFKRIPGVLET
                     RTGYANGLTENPTYQEVIGGSGHAETVKIVYNENVIHLEELILHFLRIIDPYSVNRQG
                     NDVGVQYRSGVFYPDETQRQRALAVIQQYETQQRRKTAIEVTPLAGFYDAEDYHQDYL
                     DKNPYGYCHISLRTAAEPLIPYKIHKDGSPDALRLRIGDLAYNVTQNSSTERAFSSIY
                     DNFYERGIYVDIVGGEPLFSSDAKYKSGSGWPSFTRPITADALEYVVDESYGMQRIEV
                     RSRQSGSHLGHVFDDGPRESGSLRYCINGAALRFIPYDAMEREGYGAYRIFVK"
     gene            11706..12281
                     /locus_tag="PKM49_03150"
     CDS             11706..12281
                     /locus_tag="PKM49_03150"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HNY81846.1"
                     /translation="MDILELFNMLTNDKTLDSLAGSVGATKTQTKQLVDLAMPTMMKA
                     MDRNTGVSKGADGLLKALKQHQDDDVKKMVMDFNTVDKVDGSKIVNHIFSQKTEQVEK
                     NLAKHTSLQKDQVSNVLSQLAPILLGALGNQQKGQPVDVSNLSSFLNGTMEKTGQTGM
                     MSLVESLLDKNKDGNIWDDILRFFAGLFKKK"
     gene            12634..13194
                     /locus_tag="PKM49_03155"
     CDS             12634..13194
                     /locus_tag="PKM49_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HNY81847.1"
                     /translation="MRKSNTRELALTGLFVSLTAVGSQISIPLGPVPWTLQVFFVCLT
                     GVFLPAKEAFFAQSLYLLLGLLGFPVFSRFGSGIPFLFGPTGGFLLTFPLMAFIIAKA
                     RSLAAFAATCCLSLALLYLFGALWLSFYLGSIEKALLVGVLPFLGWDLLKVFFIIRIV
                     KRTGRFRTENTEPVYAPINVRDLKDR"
     gene            13231..13872
                     /locus_tag="PKM49_03160"
     CDS             13231..13872
                     /locus_tag="PKM49_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNY81848.1"
                     /translation="MEPGKLDYRKTDKRYYLPKTDPEVIIVPAFHFFALEGEGDPNTE
                     RFARHVETLYALSYTLKMLPKSGVFPEGYSDYAVYPLEGVWSLKSERRPDYSDLFLPI
                     DKANLTYRIMIRQPDFLTSDLAQDILERTKKKKPKLPIENAEFEIYEEGRCVQMMHIG
                     TYDEESRSFAKMAEFCESHGLVRRERTHREIYLSDPRKTPPERMKTVLRWRIL"
     gene            complement(13911..14750)
                     /locus_tag="PKM49_03165"
     CDS             complement(13911..14750)
                     /locus_tag="PKM49_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY81849.1"
                     /translation="MKRSVRKRVNLVSIEIALIVITFVIFLPILLGITMSIQPPESVF
                     AFPPKLFPTGFHWRNYTEAFRKFPFEYFLMNSLIIGLATTLGKIVTGILAGYAFANFR
                     FFGKSFSFAFLFATLFLPAETIMIVPLFAIMKQFGWVNTYWALTIPFMASATNTFLFR
                     QHFRSMPKELEDAAKIDGAGYMTYFFRILIPLSKAIIGGSAIINFVYAWNMYLWPLIV
                     SMEDKMKTVQIGVKMMIDAEASNNWGIIMAGTMIALFPTLLVFFCLQSVFVKSLVRSG
                     VKG"
     gene            complement(14784..15680)
                     /locus_tag="PKM49_03170"
     CDS             complement(14784..15680)
                     /locus_tag="PKM49_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81850.1"
                     /translation="MFKRKLFHNAYGKTLPYLLLIPTFAVIILFIYWPAVSSFRMSLY
                     KVSPFGNKTIYTGLSNFENLFKNPDYEKSAWFTLFYVSFTVIATIFLSFILALLLNQK
                     VPGSRFYRTLIFSPYAVSPAISGTLWSFLLNPVVGHVNYFFMKAFNVQVLWLTTKPYA
                     LYALLFSSIWKALPFSIIFYIAALQNVSDELLESATIEGAGTLRKIWKIQFPLVSPIT
                     FYLVIMNIISSMFQSFAIIDVMTKGGPGGYTTTMMYRLFLDAFAYQKTGPASAQSVVM
                     FLLMAGVTIVYFLFGERKVHYA"
     gene            complement(15763..>16582)
                     /locus_tag="PKM49_03175"
     CDS             complement(15763..>16582)
                     /locus_tag="PKM49_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY81851.1"
                     /translation="ELYEYAKKLTVKDAAGNIIQSGITWNLNSWFFEEFMAVQNAPFV
                     DNDNGRTGRATKAVFNRPEGLVFFKLWNDLTQNGYMLNTKAVDWTAARQMFLAQKVGM
                     LITSTSDVALFTDSEANGFVTGAAFLPKPDNSPAGGVVIGGGALWIIGGHSKAETDAA
                     WKFVKFLAEEEQQIVWHLGTGYFPVDKNSLENLINSGFYAENPNLLTSIFQLLLSVQT
                     YNTNGAVIGAFPEIRNIIQNYVEKMLNKELTPEKALELAEIDSTKAIREYNELF"
BASE COUNT         4542 a         3940 c         4128 g         3972 t
ORIGIN      
        1 cggacaccga gaacggtcgt ggcgatcgcg ccaaacaagc ttgtttttat cgtcatcgac
       61 gggtaccagt cttccagcgc gggcttgacg atcgaagagc tggccagttt cctcatcagc
      121 aaagggttta ccgacgcgat gtgcttggat ggcggttctt cttccacaat ggtggtgaaa
      181 tccagggtgg tgaacaatcc gcccggagga gagcccgaaa tccctgtcgg gttactggtc
      241 aaaccgaaat aaagaaaggt tagatactat catagcatta gcgcaaaaac ggaggtgcta
      301 aagttgagtt tgccaatgga aaaacggaaa accgtcactt tgatcgggca caacgggagc
      361 gggaagagcg tgttagcgga agccttcttg gtgaaaacgg gaaaggcgga aaaaataggt
      421 gggattatga actacgaccc acttgaaata gagaggggca ccagcctttc gaacgctatc
      481 gcgaccttcg agtatggcga tacccagcta accgtgatcg acacgcccgg gttcggagat
      541 tttatcagcg aagtgattct gggggttttc gtgggagaaa acgtcttgtc catcgtcaat
      601 gcgggcgcgg gagtggaagt ccagaccgag agaacttggc ggatcgccca agagcaaaag
      661 aaacccatca tggttttcat caatcagatg gacaaagaaa gggccgattt tgaccaaacg
      721 ctcgatgatc tgaaaaaaac cttcgatata aagatgctga tactcacact gccgatggga
      781 tcaggagaga gttttaacgg tgtgatcgac gtcttgaccc aaaaagctta ccagtacgcg
      841 gccgatgggt cgggaaaaac cgccgaaatc ccgatcccgg atacgatgaa agcgcgggcc
      901 aaagaggttt atgagaagac gatcgaagac atcgttgaaa cggatgaagt ccttatggaa
      961 cactatttag gcggtgaaga gatcgaattg gaccagttgg ccgccgcgtt gaaaaaggct
     1021 tattgtagcg ggcaagtcgt acccgttttg gcgggcagcg ccaccaaaaa cgccggcacg
     1081 gaccgcctgt tggattggct gactcgattg tgcgccagcg tcaacgaagg ccacccgatc
     1141 accaccacga tggtagaagg cggcgcggag gtggaggtcc ttcccagcga gaatgagccg
     1201 ttcgtggggc tcatcttcaa agccgccgta gacccgttca tcggcaaaca atccttcatt
     1261 aagctgtacg ccggaaaaat cgcaaccggc gacggtttcg tcaataccac gaaaggttct
     1321 tatgaaaaag ccgcgcgcat ttacaccatc gtggggaaag aacaaaagga gatcagtgaa
     1381 gccggcgcgg gcgatatcgt cagcatccct aaactgaaag agagttcggt cggagactcc
     1441 ctatctcttg cggccagaaa attgctgata cctacgataa agttccctga gcctatgctc
     1501 tcgaaatctg tgaagcccat atcaaaacaa gacatcgata agatttccaa cggcttggct
     1561 cggctttcgg aatcggatcc cacctttgtt tgggagcacg acacggaaac ctcggaaacg
     1621 gtgatttcgg gaatgggcgc ggttcatttg gacgtgatga tcgagcgatt aaaacggctc
     1681 ttcaaagtgg aggtcgaggt cgggaaaccg aaaatcgctt accgtgaaac ggtcaaggac
     1741 ttcgccgacg cggaatataa gcacaagaaa caaaccggtg gccacgggca atatgggcac
     1801 gttaagatca agatgtcccc cttaccccgc gggactggat tcgagttcat acagaccatt
     1861 ttcgggggct ccgttcccta taactacatc ccgtccgtgg agaaaggagt ccgctctgcg
     1921 cttaaaaaag gcgtgttggc cggttacccg gtagtagacg tgaaggttga cctctacgat
     1981 gggtcttacc acgaagtgga ctcctcggat atggccttcc agatcgcggc gattcaggct
     2041 tttaaaaaag cgatgtctat ggcgaagccc acgttgttag agccgattat ggaggtggaa
     2101 gtcagtgtgc cggatgaaaa cgccggagat gcgatgggcg aaatcacctc ccgtaggggt
     2161 aggccgttgg ggatggaaac gggagcaaaa ggttttcagg tcgtgaaagc caaagcgccc
     2221 ttcgctgaaa tgctggactt ctcaaataaa ctcggatcga ttaccagtgg gaaagggcat
     2281 ttcacgatga agttttccga gtattccgaa gtgcctggaa acattcaaga aaagatcgtg
     2341 gcggaacgga aaagagaaca agaggaagcg aataagtaga acgtccttcc acacaagagg
     2401 cacggggggc ttgacctgtt tgaaaaccct caacagatat attatgaaaa gcgcgatgtt
     2461 tcctttcgtt ttgggtctgg gaggattcat tgtttttgtc agtatcgaat tactttacca
     2521 gctttcagag gttatcgtca gttataaagt gcctatctgg cgattatttg agctcctgta
     2581 ctacaatgtc ccgatgttta cggtgatggg cgttcccgtc ggtgttttat tggcgctctt
     2641 ttggattctc tctcaaatgg gctcgaattc cgaactgatg gctttacagg tacacggtgt
     2701 ctccttcaag aaaatcgtca tcccgttttt gatattgggc ctgatattca gcgtgttctc
     2761 gtatctgctc agcgatttcg tggtcccgga cttcaaccat cgcgcgtcag tcacattgac
     2821 caggtatgtc ttcaagcagc ccgagagcac gatcaaggcc aatcagtttt tagacgtggg
     2881 agataaccgt tatttttacg ttaagcgatt tgacaccgaa aaggcgactt tcgaggacgt
     2941 tctcctttac gacctgtctc agtcggatat gcgcatctat tacgcgcgac aagcaattaa
     3001 atccgaagac accggacgct ggaagctggt ggaagggcgg gtcttcacga tggaccgtca
     3061 gggattgatg aagctggaca ccgtctttca ggaaacggag atcgacctga cgaaagacct
     3121 cgacgaattc attcgagaca cctccaaaag agcgcaggac cttacttcca aagaactgtg
     3181 ggtaaagatc gaagcttata aaaaaatggg catccgcacg aattctttgg aggtcgagtt
     3241 tcatacccga ttcgcaaacg ctctggggcc gatcatcgtc gccttcctcg gtgttccctt
     3301 gtcactcacc ttcaatatca aaagcaaatc gtggagcgtc atcttcacct tcattttaat
     3361 cgtgctgtac cagggtagcg gagcgtggat gtccgcctta gggaaagacg gggaaacgtt
     3421 gacccccttt ctcgcctctt ggctgccgga catcggtttc gccattattg ggttcattct
     3481 cttcatcctt ttggacacga aactgatgta tcgacttaaa gagatgttca gccgattgtt
     3541 caccttttcc gtcacctcaa gagtcttcat cctcctcctc tttctttttt tttggcaggt
     3601 gacgggtttt tgtaataatc agacaacctc gaagacaggg accgatacga ccgctgtcgc
     3661 cacaccaacg gcacctccga aagcgccgaa ggaacccgcc ccttccctgc cgacgacgaa
     3721 cccgttgatc cctgcgtctg acgagcttct gataaagatc agtgccgacg agatggacta
     3781 taatggacgc gagaaaacga tcacgcttac gggcaacgtc atcatcgaat acgatcagat
     3841 gacccttcaa gcgcccagag tgaccgctta cctagaaaac gaggagtggg tagattactt
     3901 ccttgctgag tccgaaggcg cgacgaaggt gacgctcaag caagaaaaag aatactacca
     3961 ggctcaattg gtcaaagctt acgtcaaacg gaacgccttt tttttgtacc acttaagagg
     4021 gacctttaaa caaaagaacc aaaaggggca agaaaagccc ttgatcctcg tttcagataa
     4081 gagccggagc aagaccttcg tgaacaaaca cgtcaccgaa gtccaaactg gatatttaac
     4141 cacttgtgat ttggataaac cacactaccg gttcgaggcg ggctacatcg tagtcaagga
     4201 atcgaaagag atcatcgcat acgacctgtt gctctatgtt ctcaatatcc cgatattgcc
     4261 ttatccagta tatttcgccg atatgacgaa agagaagcaa ccggtcgaga tctcctttac
     4321 tttaagcgga gagggcggtc tacagacgca tttggaatac acctatctcg atcgcgcgaa
     4381 cgagaaaggc gtcatcaaat tcgacaccgt tgagaaaggg ccaaaaaaag gcgcgacaat
     4441 agtgacagaa aacaagttcg cgctcccgtt tgatatcata ggatacctga atatgactca
     4501 taaatccacc aacgatttca aggatcaaac tcgaatcctc gatttcaatg tcagtaaaac
     4561 tttcgagaat aaactcataa tgaaagccta ctataaatcc acacgtgtcc tggaaaacga
     4621 agcgacctcc aagcgtgatg gcagcgtcgg gctggagttt tccggccctt tttttttgaa
     4681 agggctcagc ttttcattga aggcgaacag gacagaaaat cgctccttga ccgcttcaaa
     4741 aaacacttac gagtttccga cctttacaat cggcagctac tcgcaggtgt tgttcaaaga
     4801 tttctatccc gtcacgatca cgttagaccc tttctccctc accggaaagg cggtgactcc
     4861 cgcggcgaca tcgattgtag aagccttgga cgtattggat tatacgactc atcaaagtat
     4921 aaagatcggt tatagcgcac ttaaggtcga cgacgtcacg ctcgttaagc ccatgaaaac
     4981 cgacctggtg ctcgacatgg atttcaacaa aagagatttc accaactatt tcataataaa
     5041 aaataccgtt ccgtttcaga atgtcggttt ccaatttggg cagaaagaca aagccttcgt
     5101 aggttttgac agcgcataca ccgtgcgttt gggatatatc cgtcacagaa acaaccaaac
     5161 ggcttggcgg tattccgaaa acgtcctgtc ggaagcatcg gtgaacctgt ggtggctcaa
     5221 tcatcaaatg acgcacaact acgtttttct catcgccgat ggagaaaact attcggcgtt
     5281 gggagaggac gtcgcagaaa gaaacgcgct cagcatgacg accatcttac aggtcccctt
     5341 tctgttttcc aagttcactt tgaagaacgg tttcgatttt atgaagaaaa gcgatcggat
     5401 gagcgacccg acattaatat cgaatacgac gatcaaagta cagcaactgt cattcgatat
     5461 gaccaccacc agtccctaca gtttggaact gaaacaattt gtcagcaccg tgtacacttt
     5521 caacctcacc catccctatg gaaactcaag cctggatttc acctacgttt acgagcagcc
     5581 ggatgagaag aataagaaga agggggagga gtggaagggg ccggtagaca aaatcacaaa
     5641 ctccaactcg ctcagattca acaacttagg tctcgtgagc tctttcaccg aagatcttaa
     5701 tttcacctgg acaacatctg ggacggcaac tgaaaccggg ggatttgagc tcaataagct
     5761 aatacaaaaa aacactctaa aattgaaagg gttcggtacc ctgaaatctc tacaaaacac
     5821 ctttgatttt gaatactttc ctcaaaaacc cgctaaagac cgttggtctt atatcaacga
     5881 tgacttatct cttgagttcg acaagttcac aactactatg actttgaaat ggaaaccacg
     5941 caagtcgatg tacctcggat tggtcggaac ttttccaggg ttctctttta agttcgacac
     6001 caattacaac attgaaacct tcgcgtttca aagtatgggg ctcacgctaa ataaagacct
     6061 ccattgctgg gaaaacacga cggaaataaa gttcgattat atcgattcaa aatttaagct
     6121 ggcgagcttc gcgaccacct tccgtataaa atccttcaca gaaaaattct ttagggccga
     6181 tccggtgaag aaaactttcg acctttccat tttttaaaag aacgaatatc cacaagccga
     6241 gaggcgcaag tttcgtgttc gacacggagc tttttaagca ggaattttct ggcaattcgg
     6301 gcaatagtat acgctaccgc ccatataagc ctctttcgcg atcgtttcgc cacatctagg
     6361 gcacggtttt ccggcagtgt ttttactcat ctgagtttgg tatccgccgc aggccccaaa
     6421 aagatctctt tcagtatccc ggcctccaaa ttcggccatt tcctttaggg ttgttttcaa
     6481 gattttatac aataacgatt tctcctcttt tgtcaaggtc ccgatttttc gcttcggatg
     6541 caaacccgcc cggaatagga tatcctgcaa aacaccgtta cccaagccgg gaatccgctg
     6601 ctctgtggcc aagaatccct tcgcaggcat tttctgcata tccggattgt cgattaactc
     6661 ctcaaaatat tgttggttaa acgcatcaga caacgcgaac ggcttcgatt tcgcgctcag
     6721 gtaatacgga ttgtcgaaac tcccgcttgg gaaacaccaa agcccgccat acatctgtac
     6781 aaccgcgctg agatacgagc cgtcttcaaa accgatgaga agctggtcct ttttcggaac
     6841 ggtagagtcc gttggacaga aggttaaacg gatgccatcc ccgaacagaa tcacagctcc
     6901 cgaggcgcag atttcgacca tactgccgat cccagtcgcg aattcgatct ttttgcccgt
     6961 taacaacttc ggatagtctt ccggacgttc gtagtaccac gccattttgt gtggcgttcg
     7021 ctggggcaac acttttgtga ccgtttttcc ccgcaaggat tgtgagattt gcttcgctaa
     7081 aacgactgat tctggtattt cgatcatacg cgacctcctg cgaaaacttt tttaaaatag
     7141 gcatcactta tcctgaaaac gattatatca ccaaaaaacc gccgtgtggg tcaagttatg
     7201 gggtgacata acaaaggggt tcattgcggg gtaaccaact ggatcccgcg aaacgccttt
     7261 tgttcttttt acatcggtcc aggagcgccc tgagaacgcc ggactttagt ctggcatgtg
     7321 ttttcttttt cccggagagc cctgtcccta gtcagctttt ccactgtgtt ctctgtgccc
     7381 tctgtgcgaa ccctgttttt caagacatat acaaaaccgt atcgcgcccc cgcggaaaga
     7441 atgagcgctt acgccgcgtt gaccgtgtgt ggttatcgcg gggtaagtgg cttttacgac
     7501 cccgcgatac gccttttgtt ctttttacat cgccccggga gcgccctgag aacgccggac
     7561 tttagtccgg cacgtctttt ctttttccgg gagagccctg tccctagtca gcttttccac
     7621 tgtgttctct gtgccctctg tgcgaacctt gtttttcaag acatatacaa aaccgtatcg
     7681 cgccgtcgca aaaagaagaa tcgcttacgc cgcgatgacc gtgtggggtt atcgcggggt
     7741 aagtggtttt tacgaccccg cgatacacct ttagcccttt ctacgcgaga tgagcctgtg
     7801 caaatgcata tcgcgccgtc gcaaaaagaa ggggcgctta cgccgcgatg accgtgtggg
     7861 gttatcgcgg ggtaagtggt ttttacgacc ccgcgatacg cccttagccc cttctttctt
     7921 ggacgcaata ggcccgtgca aaagcgtatc gcgccgtcgc aaaaagaaag ggcgcttacg
     7981 acgcgatgac tagagagaca aagaattgag cgggcgattc tggccttgtt tcttggcggc
     8041 ccctcataac gtgacgcaca cgaacgctcg aatgataaga gaactcaggt ataatcgctg
     8101 aagagaaaca gaacgaagga tacgcgccac atcgtgaaaa cttagaatag agaatccgat
     8161 tcttattcaa tgtgccggga ggtttgatga cgtgggcaac aaaagcaaca cccgattgtt
     8221 caatggcatt gcccccatct acggattatt ttatcgtcgt cagaaatccg aatttcggaa
     8281 ggtgttggaa cgcgctcacc atgttttaga tcctcggcgc tttaaaaccg tcctcgatgt
     8341 cggatgcgga acgggggctt tgtgcgcggc tctctatgaa aaaggcttcg acgtgaccgg
     8401 tatcgatccg gcagaaaata tgctgaaaat cgcgagaaaa aaggcaaggc acacccagat
     8461 tcggttcatt caagcgagcg ttttggaggg actccctttc gcggaccgtg cttttgatct
     8521 ctcgatcgcc tcttatgtcg cacacggtct gaaaccgcaa gaccggcagc gcctatatgc
     8581 ggaaatgagc agagtgacga agtcccaggt catcgtctat gactacaatg cgaaacgcgg
     8641 gctgttgacc acggtgatcg agtggttgga aagaggcgac tatttcaact ttatccgcct
     8701 cgcggaatcg gaaatgaaat cgtgtgtctt ctatttgaaa gagtgttttt cggaagtccg
     8761 ggttttggaa gtgggaaagc gggcggcttg gtatatcgga aaaccagccc gataagggcg
     8821 gcgttacccg aaattgagac gatactttta cctcgtagag cagaagatgt tttaataacg
     8881 gatcaattga caaaaccaaa aatatgagca gccggttggg aattgacgtc agaaaaagtg
     8941 gtatgattag aacaacctgc tcacgaagga ggatcaccaa atggaaaaga aatacctgaa
     9001 cccgttgatc gactatactt tcaagaaggt atttggaaat gagaagcaca aagagatcct
     9061 gatccatttt ctaaactcct taggtattgg aagccaaggg cataagatcg ccgacgtacg
     9121 catactcgac catacgattg aaaaacggtt caggccgaac aagaattcga taatggacat
     9181 caaggcaacg tttagcaacg gggagccggt gaacatcgag gtgcacatac tcccatacac
     9241 acacgtcgct cagagggcga cataccattt agcgaaaatg tattcggatc aagcggagca
     9301 aagcgatgat gacgacaagc tgcacaacac cgtcgtggtg aatgttttag ggcacaatca
     9361 gtttgagcac gacaagatcc attcggtcta tcggatgctg gactcaaaaa cgtgtgaaaa
     9421 gctttgtgat actctggaga tacacttttt agagatgcca aagattggca cgagttcgca
     9481 aaaattaggt aaagagctga cgggctggtt attattcttg accgacccgg gaaaccaacg
     9541 gaccattgag cttgggaagg aggtaggaga gataggacaa gcgatcaacc ttttagaaga
     9601 actgagccag agccgagaag agcgggcgat agcggaagag cgtgagaaag cgcttaacga
     9661 tgagcgttcc tttgggaacg tcgaataaaa aaaggatata gacaatggcg ggagaaagag
     9721 taatttttta atcgccaaaa agcacgaact taaagtgaag gacttatcgg aggtctcggc
     9781 gatcacagga gtatctctcg acaaccagat gagcatcgat atcacagggt tgtagaaacc
     9841 gggcggtcct tctcgttcgc cgatcgttct atctagaaac acgcgaggga tactagacag
     9901 acaaatccgg gcggttgatc gtattagccg gaagaaagac ctttttccct caatgcccta
     9961 tcttttcgaa ctttactgtt tgtgaggttt gcaaaaaacc cataagaccc tctattggta
    10021 atcggtttta aactggataa gaattcaggt gtcttcgata gtagtcaagc tcgagcgcat
    10081 ccggctcagt ggccaaaata gcgggtagga gcgctttata accgtcaagc ccgctttttg
    10141 aagtaggtga ctttcttgaa atcgatccta tggataatag tctttctttc agtgatactg
    10201 atactcgttt ttgctcaagc gaacttgatc gcagaaaaaa aggagacaac gatgactagc
    10261 gacggaatca gagagtactt atggaaaaac gcccagaaag acaagagaat actgtacttg
    10321 gctggcgggt gtttctgggg tgttgaaggc tacttcaaaa gaatccccgg ggtgttggag
    10381 acacgtaccg ggtacgcaaa cggattgacg gaaaatccaa cctaccaaga ggtcatcggc
    10441 ggctcaggcc acgcggaaac ggtaaaaatc gtatacaatg agaacgtcat acacctcgag
    10501 gagttgattc tgcatttttt aagaatcatc gacccgtact cggtgaaccg tcaaggcaac
    10561 gatgtcgggg tacagtatcg ttccggcgtt ttctatcccg atgaaactca gcgtcagagg
    10621 gctctcgccg tgatccagca atacgaaacc caacagagaa gaaagaccgc gatagaggtc
    10681 acaccattgg ccgggtttta tgatgccgaa gattaccacc aagactacct cgacaaaaac
    10741 ccttacggtt attgtcatat ctccctgaga accgcggctg aaccgctaat cccttacaag
    10801 atccacaaag acggttctcc agacgccttg agactcagaa tcggtgatct cgcatataac
    10861 gtgactcaga attcttccac cgagcgggct ttctctagta tctacgataa tttctatgaa
    10921 aggggcatct acgtggacat cgtaggaggg gagccgcttt tctcttcgga tgcgaaatac
    10981 aaatccggaa gcgggtggcc cagtttcacc aggccaatca ccgccgacgc cttggaatac
    11041 gttgtagatg agagctatgg tatgcaaagg atagaagtgc gcagccggca gagcgggtcg
    11101 cacctgggcc acgttttcga cgacggcccg cgcgaaagcg gctccctccg ttattgcatc
    11161 aacggcgccg ctttgcgttt cataccgtac gatgctatgg aaagagaagg gtacggcgcc
    11221 tacagaattt tcgtgaagta gtctgccgca gaagttgcta cgcgatcaaa ccctacacga
    11281 aaagcgcacg acacgtacgg tttatctttg gcgcggcgat atgcttattg tgtgggaata
    11341 atatcgatgt gtagcaaaaa aaaagcgaat gaggatgacc gtatgtgtga tcgcgcagca
    11401 agtggttttt acgactccgc gaaaaacctg ccaacttaaa aggtggcgtt ctcagagaac
    11461 atgccgaact aaagttcggc gttcccaggg cgttcccagg agttccctag cgtatcgcgc
    11521 cgtcgcgaaa agaaggggcg cttacgccgc gatgactcca tagaccaaaa atcgtgcgga
    11581 cgacgctggt cttgtttctt ggtgatacct cataacttga cacacactgt atatttatta
    11641 tgtcgtttta tgaggtatag tatgaaataa tatatactgg tataagcgaa aaacggaggt
    11701 gcgtgatgga tatcttagaa ctgttcaata tgctaaccaa tgacaaaacg ttggatagcc
    11761 ttgctggtag cgtcggggcc actaaaacgc aaacaaaaca attggtggat ttggctatgc
    11821 ctacaatgat gaaggcaatg gatcgaaaca ccggcgtgtc gaaaggggcg gacgggctgc
    11881 tgaaagcgct gaaacagcac caggatgacg atgttaaaaa aatggttatg gacttcaata
    11941 ccgtcgacaa agtcgatggc tcgaaaatcg tcaatcatat tttctcacaa aaaaccgagc
    12001 aggtcgaaaa aaacctggct aagcatacgt cgctgcaaaa ggatcaggta tccaacgtgc
    12061 tcagtcaatt ggcccctatc ttgctcgggg ctttgggcaa ccaacagaaa gggcaacccg
    12121 tggatgtctc caatctatcg agttttttga acggcacgat ggagaaaacg ggtcaaaccg
    12181 ggatgatgag cttggttgaa tcgttattgg ataaaaacaa agacgggaac atctgggacg
    12241 acatcctacg ctttttcgcg ggcttattca aaaagaaata agccaaaccg tagttttata
    12301 gcgtgaattg cgagtaatgc cgctcaacac cctctatgga tcttgtttcc ttgtaagcgt
    12361 ggcttgaaac tgtagagcta gcaactagat tcgggattgg ccatatttgc gtgcgaacga
    12421 gggaacttgg gtttctcgaa atgttctcag aggaggaaaa atcgctttgg gtcggaagac
    12481 ccacgccggt ccgagtacct atccggtcga tctgttgcgt ttgagaggta catacgaccg
    12541 ggcgagaagg aagaggtttc tatagacaag gatagcctgg tgtccacaaa ggatttttcg
    12601 atgaaaccta taaggtcgag tgtagataat atattgcgaa aatcaaacac ccgtgaactc
    12661 gcgctcacgg ggctattcgt ttccctaacc gcggtagggt ctcaaattag tatcccgctg
    12721 ggtccagtcc cttggacatt gcaagtgttt ttcgtttgtt tgaccggcgt cttcttgccc
    12781 gcaaaggaag ccttcttcgc gcagagcctg taccttcttc tcgggctctt gggttttcca
    12841 gttttttcaa gatttggttc gggaattccg tttctttttg gccctaccgg cggtttcttg
    12901 ctcacctttc ccttgatggc gtttataatc gcgaaagcac gaagtcttgc cgcttttgcg
    12961 gcaacttgtt gtttaagcct cgccctgctc tatctcttcg gagctctttg gttgagtttc
    13021 tacttgggaa gcattgagaa agcgcttctc gtcggcgttc ttccgttctt gggctgggac
    13081 ctcttgaaag tgttcttcat catacgaatc gtaaaacgca ccggaaggtt tcgaactgaa
    13141 aataccgaac ccgtctacgc tccgataaac gttagagatt taaaagaccg ataggctgag
    13201 cctattcggc taaaaaagga ggagaaactg atggaaccgg gaaagttaga ctatagaaaa
    13261 acggataaac gctactatct tccgaaaacc gacccggaag tcatcatcgt tccggccttt
    13321 cacttttttg ctctcgaagg ggagggtgat ccgaacacag aaaggttcgc ccgacacgtc
    13381 gaaactttat acgcgctgtc ctatacgttg aaaatgctcc cgaaatccgg tgtttttccc
    13441 gaggggtata gcgactacgc agtctaccct ttggaaggtg tgtggagtct caaatctgaa
    13501 aggcgtcccg actattccga cctgtttctt ccaatcgaca aagccaattt gacctatcgc
    13561 atcatgatac gtcaacccga tttcctaacc tcagatttgg cgcaggatat cttggaaagg
    13621 acgaagaaga aaaagcccaa actgccgata gaaaacgccg agttcgaaat ctacgaagag
    13681 ggacgctgcg ttcagatgat gcatatcggc acgtacgatg aggagagccg aagtttcgca
    13741 aaaatggcgg agttctgtga aagtcacgga ttggtacgaa gggagcgcac ccatcgggaa
    13801 atctaccttt ctgatccaag aaaaaccccg ccggaacgga tgaagaccgt tctacgctgg
    13861 cgtatcttat gaaaaagctt atgtatagga aatattacaa cgaaaagggc ttagcctttt
    13921 acacctgagc ggaccaaact tttcacgaac acgctttgca gacaaaagaa aacgagcaag
    13981 gtcgggaaca acgcgatcat cgtgccggcc ataatgatcc cccaattgtt ggaagcctcc
    14041 gcgtcgatca tcattttgac cccgatctgc acggttttca tcttatcttc catactgacg
    14101 atcagaggcc agagatacat attccaagcg tacacgaaat tgatgatcgc cgatcctccg
    14161 atgatggctt tcgagagagg gatgagtatc ctgaagaagt aagtcatata tccggcgccg
    14221 tcgatcttcg ccgcgtcctc cagttctttc ggcatcgagc ggaagtgttg tctgaatagg
    14281 aaggtgttgg tcgccgaagc cataaacggg atggtcaacg cccaataggt attgacccaa
    14341 ccgaactgtt tcataatggc gaacaacggg acgatcataa tcgtctcggc aggcaagaac
    14401 aaggttgcga aaagaaacgc gaaagaaaag ctctttccaa aaaaacggaa gttggcgaag
    14461 gcgtaaccgg ctaagatccc cgtgacgatc tttcccaagg tcgtcgcgag cccgatgatc
    14521 aagctattca tcaagaaata ctcgaaaggg aattttcgaa aagcctcagt gtagttgcgc
    14581 caatgaaacc cagtgggaaa tagttttggc gggaaagcaa acacgctctc tggcggttgg
    14641 atgctcatcg tgataccgag taaaataggt aaaaaaatca cgaatgttat gacgattagg
    14701 gcgatttcga tactgactag attcacacgt ttcctgaccg atcttttcat cgcacacctc
    14761 aaaccgcttt ctgtgtcatt attttatgca taatgcactt ttctctcgcc aaagagaaaa
    14821 tagacgatcg tgactccggc cattaacaag aacatcacga cgctttgtgc ggaagccggt
    14881 ccggttttct gataagcgaa ggcatccaaa aaaaggcgat acatcatcgt cgtcgtataa
    14941 ccgcccggac cgcctttggt cattacgtcg atgatggcga aggattggaa catcgaggag
    15001 atgatgttca ttatgaccaa atagaaagtg atcggagaca ccaacgggaa ctgtattttc
    15061 cagatcttgc ggagggtacc ggcgccctct atggtcgcgg attctagcaa ttcatcggac
    15121 acgttttgca gcgcggcgat gtagaagatg atgctaaagg gcaaggcttt ccagatgctc
    15181 gaaaatagca acgcgtagag agcgtatggt tttgtcgtca accacagcac ttgcacgttg
    15241 aaggccttca taaaaaagta attcacgtgc ccgacaaccg ggttgagcaa aaacgaccag
    15301 agcgtcccgg atatggccgg cgaaacagcg tagggtgaaa agatcaaagt tcgataaaat
    15361 ctggaacccg gcactttctg attcaagagc aacgccaaaa tgaaggatag gaagatcgta
    15421 gcgataaccg tgaaggaaac gtagaacaaa gtgaaccaag cggatttttc ataatctggg
    15481 tttttaaaga gattctcgaa attggacaat ccggtgtaaa tcgtcttgtt accgaaaggg
    15541 gataccttgt ataaactcat gcgaaacgag ctaaccgcag gccaataaat gaagagaatg
    15601 atgaccgcaa atgtcggaat caataataga tacgggagcg tttttccata ggcgttatga
    15661 aagagttttc gcttaaacat actttccccc tctatccaaa ataccgggga ctgctgaaaa
    15721 ggcagtcccc gacatctaat ccgcctgatc gttatactgg aatcagaaca gttcgttgta
    15781 ttctcggatg gcttttgtgg aatcgatctc ggctaattcc aacgccttct cgggcgtgag
    15841 ctctttattc agcatttttt ccacatagtt ctggatgatg ttccgaattt ctgggaacgc
    15901 gccgatcacc gcgccgttgg tattgtaggt ttgtacagac agaagcagct ggaagatcga
    15961 ggtgaggaga ttcgggtttt cagcataaaa accggaatta atgaggttct ccagtgaatt
    16021 cttatccaca gggaagtatc cggttcccaa atgccacacg atctgttgtt cttcttcggc
    16081 caaaaatttg acgaacttcc aagccgcgtc ggtttccgcc ttcgaatgac caccgatgat
    16141 ccacagagcg cctccgccga tgacgacccc gcccgccgga ctgttatccg gtttgggtag
    16201 gaacgcggct ccggtaacga aaccgttcgc ttcggaatcg gtgaacagcg cgacgtccga
    16261 cgtagaagtg atcaacatcc ccactttctg tgccaggaac atctgtctcg ccgcggtcca
    16321 atccactgcc ttcgtgttca gcatataccc gttctgcgtc aaatcgttcc acagtttgaa
    16381 gaataccaac ccttccggtc tgttgaagac tgccttcgtg gctcggcccg ttcttccgtt
    16441 gtcgttatcc acaaacgggg cattttgtac agccataaat tcctcgaaga accaggaatt
    16501 caagttccac gtgattccag attggatgat attgcccgca gcgtccttta ccgtgagttt
    16561 ttttgcgtat tcataaagct cg
//
LOCUS       DAOPXN010000034        16445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3585_length_16445_cov_4.826174, whole genome shotgun sequence.
ACCESSION   DAOPXN010000034 DAOPXN010000000
VERSION     DAOPXN010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16445
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3585_length_16445_cov_4.826174"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PKM49_03180"
     CDS             <1..887
                     /locus_tag="PKM49_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HNY81852.1"
                     /translation="SSFRGFAVDQIDYEVFEYLGDLLGPVKVNILTTLEYEDTQQNLK
                     IHFEPGLQALNGKQLLLYIRFRNDHLGDLGRIQRQKEVLLTLLQSLKTKKDTLTLNRL
                     IQDVLGKIETNFDAIDLLSLWGYADALGHVAFLNFPYLINAKGEIEVSSEKIWRVCEE
                     LAKMEPVQEPYAPQMVLINAWNDNAYQFSIVEYNLWSSQSIRPFIIEKRLVHEAVKKL
                     ASTRDLLVFLVKDADKQKEITERYQRVYPKRPFLAVFPEIGAVFSLKDYLSVVDALMK
                     AKAAFPFPADALIIRTQP"
     gene            900..2309
                     /locus_tag="PKM49_03185"
     CDS             900..2309
                     /locus_tag="PKM49_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNY81853.1"
                     /translation="MLQKRDIEEVLSVLLETGGDFGELFFEDTRSAEMSMTDGKLEKV
                     NTGRLFGVGLRAFLETKAVYAYTNDLSIDNLKATARRIGKALKGPSKGYESVTLSEKH
                     HQTKLPVLTPFEIMERQEKKRVMRLAHEGARTFAPAGSALNDLIGQVEVKYLEKDQYV
                     RIANTEGVFTEDRRRRTRLITTVIATEYGQKETGSSSPGSGAGFEFYNLIDPKEVGRE
                     AARIALRMIRADFSPAGMMPVVIDNGFGGVIFHEACGHSLEATAVAKGASVFCGKVGQ
                     KIAADCVSAVDDGTIPNAWGTCTVDDEGMPTQRNLLIENGVLKGYLIDHINARKMGMK
                     PTGSGRRENYTFAPTSRMSNTFLLPGYSIPAEIIANTEWGLYAKTMGGGSVQPTTGEF
                     NFAVLEGYLIEKGRVSKPVRGATLIGKGHEVLEKIDMVGNNLARSQGMCGSISGMVPA
                     DVGQPTIRVSALLVGGRNQ"
     gene            2306..3673
                     /locus_tag="PKM49_03190"
     CDS             2306..3673
                     /locus_tag="PKM49_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNY81854.1"
                     /translation="MNFEAFRNSIFDIAVKKGLKTFELFGMEQRELEISVFESQIEHY
                     KDASCAGASFKTIVDGKAGSSFTEVWTPESAARLVEDAYENCQQIDALDPEYIYTGQE
                     PYRTFPPFADRYAQLPIEEKIAYAITMEKEAKAYHSAIVNIPYCTYFEGADRFYVSNS
                     YGVNVEYGREGGGGYLVAAASDGKTAKTGLDFVAQASPVGLDFRATARKAAGKALKKL
                     GAKSVRTGKYPVILDYNMVGMLLTCFSFMINAENAHKGLSLLAGKLGQVVTAPVITIA
                     NEPYIEGGISNTPFDAEGVPTKTTTIVRNGVFETFLHRMKTAVKDGLSSTGNAARVRY
                     DQATVIAPQNLVLRPGLLNLDELIIQMGDGLYITDLQGMHSGANPYSGQLSLGAEGFK
                     VEGGKIAYPVEQITIAGNLLEWFGRVVDLSSSSNLSMPSGNFSVYCPDMYISEIDVAG
                     TESVS"
     gene            3679..4263
                     /gene="coaE"
                     /locus_tag="PKM49_03195"
     CDS             3679..4263
                     /gene="coaE"
                     /locus_tag="PKM49_03195"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNY81855.1"
                     /translation="MGITGSIASGKSEVSRYYERKGYPVVYEDEVGHLALRDAHIMAE
                     TVRAFGTSILGVHGDIDRKALGRIVFSDPQALLRLNAIVHPWMIDHTLARFQVLEDAH
                     HPLVFLEAAILFEMGLDASVDWVIFVDATRKTRIQRLVETRGYDVEEARKRVMAQRVG
                     RHKSKSHFILRNEGTLTALHKRCGRLLIVLLAKE"
     gene            complement(4277..4681)
                     /locus_tag="PKM49_03200"
     CDS             complement(4277..4681)
                     /locus_tag="PKM49_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNY81856.1"
                     /translation="MDFSDIGIGMINRQTFFVSEERVRAFAELTGDMNPVHLDEEFAK
                     STQFGRRIAHGMLVSSFISKVLGRDFPGDGTIYVAQKVRFRRPVFIGDTVVVEVEVTE
                     KNEEKRRLILRTDVINESGEKVIIGEAEVMKP"
     gene            5144..6763
                     /locus_tag="PKM49_03205"
     CDS             5144..6763
                     /locus_tag="PKM49_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81857.1"
                     /translation="MKRTIGLAILLFVFTLFFYGCAYIDIGPTIEAGFRHFNSGDLRT
                     AENCFREALRRNPTRPQANLGLSLVTLFRGNQRLISIFKRIGPIDYALPGALRANAKS
                     ALKAELQKRLLNWFFKTRVLRAEDDLDLSWIRWWIQEGQREIAETVALLEEAKSCLER
                     ALASPEPVDIFPNHLDWNQNGVLEPETPLLFSWFAQGAPVWKIIAEALIGKNELPPDE
                     KISRFFDPSSGDAWFDKELFDLIRSGEIDELDLDAWEPVFDEEDVLTIGSTEMRWLYL
                     LVTGELSALEPLVIYQLDFGSALQDFLNRFSKINREEGIESAYAYAADFLDANHDGKI
                     SNLEWCALFDEGFLAFRPEEENGGTHAVENWKGAIVGFCETMIWMIENDQLPFLDDPE
                     EEGPFGLNLARLDTGSPLSERLLAILRETIDYVSDPTKAVTLFSIESEEPLFSRVRGF
                     LDLRGIDSRFIDELHVLAYPGMYFAHPENFSDLKAFFPQLYYQWIDRTFPIPELFLFE
                     DPTFGGLLQADINGHPWNGVFSQIFFLITKR"
     gene            6773..7276
                     /locus_tag="PKM49_03210"
     CDS             6773..7276
                     /locus_tag="PKM49_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNY81858.1"
                     /translation="MKANPKTNAIRFLERHRVDFESRVYEVDESDLSAVSVAQKLGIR
                     VEQTVKTIVLKGNRSGVFVCLLQGHRDVDIKRLASFVPDTMIGTVDAGRLLSLTGYVR
                     GGVSPFGMTKTYPFFLDRPVLREEWISCSAGKKGIQIWMRSSDLAALTGATILDFSKD
                     QQPHIKG"
     gene            complement(7261..8337)
                     /locus_tag="PKM49_03215"
     CDS             complement(7261..8337)
                     /locus_tag="PKM49_03215"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY81859.1"
                     /translation="MDFYLNVTVAGLETTRQICSDYGYWFRRGDETSLTLFFTDRALV
                     SEVRRVVDDLKGPGSAPVHLIADPVPFHELHRFYRKKGLLNEWIPYRPGDLFRIHRLL
                     EPNAGNQSYETQPVSIQFDVRYDQTVPEKRRVYRREQIAAILDAIDHLHPTSLIDWGC
                     GCGANYSFLKDKLAQEGIRYQGLDTSRFLISKAKDLYETPESDPRVRFDLGDARRLPY
                     PDHAFDLGFSESLLPLVPNPLAALTEMARTTLFGFFSSLYTIASHQPGLVYLQKERAY
                     ALDVGATWKYRKGVSEQVFYLPKTADVRAFARKVPHIVTVENKRHQFFDALGIRTVSV
                     FFYPKRWFDQRRARFTLWNYQPLM"
     gene            complement(8347..8934)
                     /locus_tag="PKM49_03220"
     CDS             complement(8347..8934)
                     /locus_tag="PKM49_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY81860.1"
                     /translation="MSKRDTKRDILQAAKAVFASKGYEASTLEDIAEKAGVRKALIFY
                     YYPSKESLFLEAWNESIDELENTLFRELADESVYIRKLKKLFRGYIDFVMSRREVMKL
                     IEIDRVRIMDGPQDGDEWGRFRLRYNRFLQKIEELIEEGKEKKALPSDISTKATAKLL
                     AQMMGMGALEENITLERIIRIILSGMALEPERILL"
     gene            9695..11140
                     /gene="gatA"
                     /locus_tag="PKM49_03225"
     CDS             9695..11140
                     /gene="gatA"
                     /locus_tag="PKM49_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNY81861.1"
                     /translation="MEIWKNTVESLVRGIRDKSWSPRDILEAFYKHAWAADSRILAFI
                     RMPKLSEISIPDALDSLPLPFAVKDNIMVEGFQTTCGSYALWNYKAGYDATAIARLKA
                     SGAVFFGKTNMDEFAMGSSTKYSAFFTTKNPWDYYRIPGGSSGGSAAAVAAGCVPFAL
                     GSDTGGSNRLPAAHCGIVGYKPSYGLVSRYGLVSLASSLDQIGPLCRSVRDAACIGNI
                     LIGKDEKDATTVDRPMDLLKGIENSIAGKRCAVVRQSVPETIDPHVRRAFWEAVEIFK
                     QNRCVVEEIDFPEERYAIATYHILVPSEASSNLARFDGVRYGFRIPEESLTDMMAESR
                     QYGFGEEVKRRMLLGAFYLGEAQYDSHYQKAMKVRRLISQRVHQIFQDYDVLLSPTSL
                     FSPFRITENTDPLAFYLSDKNTVLANLVGLPALSIPMGFANGMPCGLHLQCKPFADEM
                     LFNIARAYEKSAGFFEDDHYPFPFQEERLWL"
     gene            11131..12585
                     /gene="gatB"
                     /locus_tag="PKM49_03230"
     CDS             11131..12585
                     /gene="gatB"
                     /locus_tag="PKM49_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNY81862.1"
                     /translation="MAIKPQIGLEIHAQLLTKTKAFCSCSSESFDAPPNTHICDGCTG
                     QPGTLPKLNATVVEFAVKAALALNCHINERSSFDRKNYFYPDLVKGFQITQYFRPIAE
                     NGSLLIHADGRKKKVRIRRVHIEEDTGKLLHSPADRPECAFIDYNRCGIALLEVVTEP
                     DLDTPKEAHAFMETLRNTFRTLGICSGDMEKGALRCDANISVRDTDRGLVTARVEIKN
                     VNSFRFVEKALEYEFERLKAALLSGEKTVSETRGWDQGSRSTVSLRSKGEENTYRHFP
                     EPDLPDIVLERAWIDRIRTTIPELPMEKQDRLMEQYGLTMAEAKTFLDEPELETFFVA
                     AVGQGLPVKAVKNWLLGEIQKTLKERALTIDRTTLTVARLAEIIEWIATGKTTTHIAK
                     TLIPDLLENDISLEQLLSEKGISRINDSAYVIEVSKKVVEEHPEQAEAYRFGKTGLLK
                     YFIGLVMKETKGQCDTSLVQETIKALLEGNNIEG"
     gene            12575..12835
                     /locus_tag="PKM49_03235"
     CDS             12575..12835
                     /locus_tag="PKM49_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81863.1"
                     /translation="MRDSDAGFWNRVPASSESEGKGQAAEESDLLRIERKIDRIQCFL
                     NDNYRELEESLKKQKSYFNALEIMFYILLGISLAQFIFLMIR"
     gene            12848..16102
                     /locus_tag="PKM49_03240"
     CDS             12848..16102
                     /locus_tag="PKM49_03240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY81864.1"
                     /translation="MILPVELTMIRFSEEYSVILKEIREKNHLTRKVPKSLWKVLDFE
                     FATEKDAVVITGGFAEREIYVIAIVSFKDRAISLHCSCGYDTRIERLCPHLLFLLEEA
                     GRQSSKVEQLYEEHKRIQMFESGYRKYLGNKEQLTHLLSFFHTEQRNSSEKGAKSEEE
                     KFVVLLNPCNRELLARVARRKRNAKGKYLATGQKLVSYQRLYKRDDPFFARVLSCMSY
                     VPPSTDSYHNEFSLEITDFQRFVEAFRDDDCVYINYLNEPAVFGPTLTLHLKPKRSGI
                     KAPDQQLLEFRLINEENRIEYPLKATKTKDYFLPAFAYYGKLGLKLAIITNKIFPVVT
                     SLDAETTFRLQELLKDYDWGAEKEENKGSLQRLLFLPLLSLQVNVAPQLFGNGMAYTS
                     EKPKALWQIQSYHWNYRFQLFFKYGDRRLSAEQPESIIISETTGQIFKRDLEFEAQHI
                     QTIRNMLSHETCLDAESLLYDLHLESVLRLVNEEFPKYEKEVVLECDTRLFSRGRVKP
                     IVKTFFSYGTDWFDLALSGELNGEPIAAEDLDRLLANPSQYLQINGEYFSVDPHEAGK
                     LETLRSRLSQLKDGLRIPKADFETLQTLREVTTPSFDERTERFLSAIDKFTQIQEYPV
                     PSDFGASLRPYQIAGYNWLRFLEEYGLSGILADDMGLGKTVQALAMLHDFKRRYGRLS
                     ALVVAPKSLLRNWEAETNRFSPDLTTLIYHGYGRQNQDISKSTSDLILTTYATLRNDI
                     RLFTPKTFNYVILDEAQNIKNKNTRTFKELTKVQAENRLALSGTPIENGLSDVKSIFD
                     FLMPGFLGADKLFFSTYNENFEELGKKIAPLILRRKKEDVLAELPEKTIENLYVDMTK
                     SQSDIYDTYFKAAQSEALSIMRDEAGFQRSRFLILTLLLRLRQICCHPRLTKEELSGK
                     GLSGKLEALRELVDSICAENHKVLVFSQFSKMLEIIQEDLSDYKDQILTMTGKTQNRQ
                     EIIDAFKENPETRIFLMTLKVGGVGLNLTEADYVIIFDPWWNPASEAQAIDRTHRIGQ
                     NKPVFVYRLIAKDSIEEKVLELQKRKQSVADGVLSGSERDGGSFGKEELTYLFS"
     gene            complement(16108..>16445)
                     /locus_tag="PKM49_03245"
     CDS             complement(16108..>16445)
                     /locus_tag="PKM49_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81865.1"
                     /translation="VFRALFTHGYYLLPPVFDPVIEEALFVLSLAGGGLVSFVCFKLL
                     PVHRGPIHTFLFGLVYALALGALYWYLFENLSTEKAALSRLFFIFMCALLGFLNHLLL
                     DSLSGRLKR"
BASE COUNT         4158 a         4127 c         4371 g         3789 t
ORIGIN      
        1 ccagcagctt ccgcggcttc gcggtggacc agatcgacta cgaggtcttc gagtacctgg
       61 gagacctcct tggccctgtg aaagtgaata tactgacgac gttagaatac gaggacactc
      121 agcaaaactt gaagattcac ttcgaaccgg gacttcaggc gctgaacggg aagcagcttc
      181 tgttgtacat ccgttttcga aacgaccatt taggagactt gggaaggata cagcgacaaa
      241 aagaagtcct gctcaccttg cttcagtccc tgaaaacgaa gaaggacacc ctcacgctca
      301 accgtctcat acaggacgtc ttgggaaaga tcgaaaccaa cttcgacgcc atcgacttgc
      361 tctccctttg gggatacgcg gacgctttag gacatgtcgc ctttttaaac ttcccctatt
      421 tgatcaacgc gaaaggggag attgaggtct cctctgaaaa aatctggcgg gtgtgtgaag
      481 aactcgcgaa gatggaacca gttcaagaac cttacgcacc gcagatggtc ttgatcaacg
      541 cctggaacga caacgcttat caattctcga tcgtggaata caatctctgg tcaagtcaga
      601 gtattcgccc cttcatcatc gaaaagcgtc tcgttcacga agccgtgaaa aagctggctt
      661 ctacccgcga tttattggtt tttttagtca aagacgccga taagcagaag gagataacgg
      721 agcgctatca aagggtctat ccgaagcgac cctttttggc tgttttcccc gaaatcggcg
      781 ccgtcttttc acttaaggat tatctatccg ttgtggatgc gctgatgaag gcaaaggccg
      841 cttttccttt ccccgcggat gcgctgatca tcaggacgca gccctgaaag gaggtcaata
      901 tgcttcaaaa gcgagacatc gaagaggtct taagcgttct gttggagacg ggtggagatt
      961 tcggggagct gttctttgaa gacacgcgat cggccgaaat gtcgatgacc gatggcaaac
     1021 tcgagaaggt gaacacaggc agactcttcg gggtcgggtt gagggccttc ttggaaacga
     1081 aggcggttta cgcctacacc aacgatctct cgatcgacaa cttgaaagcc acggcccgaa
     1141 ggatcggcaa agcgctgaaa gggccgtcca aagggtacga atcagttacc ctatctgaaa
     1201 aacatcacca gaccaagtta cccgtgctga cgccctttga aatcatggaa agacaagaaa
     1261 agaaacgggt catgcggctg gcgcatgaag gcgcgaggac cttcgccccc gcgggaagcg
     1321 cacttaacga cctgatcggc caagtcgaag tcaagtattt ggaaaaggat cagtacgtgc
     1381 gtatcgccaa taccgaaggg gtgttcaccg aggatcgcag gcggagaacc cgactcatca
     1441 cgacggtcat cgcgaccgag tacggacaga aagagacggg ttcgagttct cccggatccg
     1501 gagcgggttt cgaattctac aacctcatag acccgaaaga ggtcgggaga gaggccgccc
     1561 gaatcgcgct tcgaatgata cgcgctgact tctcgccggc agggatgatg ccggtagtga
     1621 tcgacaacgg attcggcggt gtcatttttc atgaagcgtg cgggcactcg ttggaggcga
     1681 cggccgtcgc gaaaggagcg tcggtcttct gtgggaaggt tggccagaaa atagccgccg
     1741 actgcgtgag cgcggtcgac gacgggacca tcccgaacgc ttggggaacg tgcaccgtcg
     1801 atgacgaggg gatgcctacc caaagaaatc tgttgatcga aaacggcgtg ctaaaaggct
     1861 acctgatcga tcacatcaac gctcggaaga tgggtatgaa acctaccgga agcggacgac
     1921 gagaaaacta caccttcgcg ccgacctcgc ggatgtccaa taccttccta ttacccggat
     1981 actccattcc ggcagagata atcgccaaca cagaatgggg gttgtacgcc aagacaatgg
     2041 ggggcggatc cgttcaaccg acgaccggtg agttcaattt cgccgttctc gaagggtacc
     2101 tcatcgaaaa aggtcgtgtt tcgaagcccg tacgcggggc gacgcttatc gggaaaggcc
     2161 acgaagtgtt ggaaaagatc gatatggtcg gaaacaacct ggcgagatct caaggaatgt
     2221 gcgggtccat ctccgggatg gtccccgcgg atgtcgggca accgacgatt cgtgtgtccg
     2281 cattactcgt gggggggaga aaccaatgaa cttcgaagcc ttcagaaact ctatctttga
     2341 catcgctgtc aaaaaaggat tgaagacctt tgagctcttc ggtatggaac aacgggaatt
     2401 agagatcagc gttttcgaaa gccagatcga acactataag gacgcatcct gcgctggagc
     2461 gagctttaaa acgatcgtag atggaaaagc aggctccagt tttacggaag tatggacacc
     2521 cgagtcggcg gccagactcg tcgaagatgc atatgaaaac tgtcaacaga tagacgcttt
     2581 agaccctgaa tatatctata ccggtcaaga accctataga accttcccgc ccttcgcgga
     2641 tcggtacgcg cagctgccaa tcgaggagaa gatcgcctac gccattacta tggagaaaga
     2701 ggcgaaggcc tatcactcgg ctatcgtgaa catcccctac tgcacatatt ttgaaggcgc
     2761 ggataggttt tacgttagca actcgtatgg tgtgaacgtg gaatacggtc gcgaaggcgg
     2821 gggcggatac ctcgtcgcgg ccgcttccga cgggaaaacg gcgaaaactg gtttggactt
     2881 cgtcgcgcaa gcgtcgccag tcgggctgga ttttcgcgca acggcccgga aagccgcggg
     2941 aaaagccctg aagaagctcg gcgcgaaaag cgttcgaacc ggaaaatacc cggtcatttt
     3001 agactacaat atggtcggga tgctgttgac ctgcttttct ttcatgataa acgcggaaaa
     3061 tgcgcataaa ggcctctcgc tgttggcagg aaagttgggg caagtcgtta cggctccggt
     3121 aatcacgatc gcgaacgagc cgtatatcga aggcggaatc tcgaacaccc cgttcgacgc
     3181 ggaaggcgta ccgacgaaaa cgaccacgat tgtccgtaac ggggttttcg aaaccttctt
     3241 gcaccggatg aaaacggctg tgaaagatgg gctgtcgtca accggaaacg cggcgagggt
     3301 taggtacgat caagcgacag tgatcgctcc ccagaacttg gtattacgac ctggcctcct
     3361 gaacttggac gaactgatca tacagatggg ggatgggctt tacatcacag acctacaagg
     3421 gatgcactcc ggcgcgaacc cctactcggg acagctatcg cttggtgcag agggtttcaa
     3481 agtggaaggc gggaagatcg cctacccggt ggagcagatc acgatcgccg gaaatcttct
     3541 ggaatggttt gggagggtag tagacctcag ttcgtcctcg aatctgtcta tgccttccgg
     3601 aaacttcagc gtctattgcc ccgacatgta tatttctgaa atagatgtcg cgggaacgga
     3661 atcggtttct tgaagaaagt ggggataacc ggatccatcg cctccgggaa atcagaagtg
     3721 agccggtact acgaaagaaa agggtatccg gtggtctatg aagatgaagt ggggcatctc
     3781 gcgttgcggg atgcccatat catggccgag acggtccgag catttgggac gagcattttg
     3841 ggagttcacg gcgatataga ccggaaagcg ttaggccgta tcgtcttctc cgatcctcaa
     3901 gcgttgcttc gactcaatgc catcgttcat ccgtggatga tcgaccacac gttggctcgc
     3961 tttcaggttc tggaagacgc ccatcatccg ttggtctttc tggaggcggc gattcttttt
     4021 gagatgggct tggacgcatc ggtcgattgg gtcattttcg tcgacgcgac gcgcaaaacc
     4081 cgcatccaac ggctagtcga aactcgcggt tatgacgtcg aggaagctcg aaaacgtgtg
     4141 atggcgcagc gtgtcggtcg gcacaagtcg aaaagccatt tcatcctacg caatgaaggt
     4201 acgttgaccg cgcttcacaa acggtgcggc cggctcttga tcgttctatt ggcgaaggaa
     4261 tgaaaaggtc ggggcgtcaa ggcttcatca cttcggcttc tcctatgatc accttttctc
     4321 cgctctcgtt gatgacgtcg gtccttagga tgagccgtcg cttttcctcg tttttttcgg
     4381 tcacttccac ctcaacgacc accgtatccc ctatgaagac ggggcgtctg aatcgcactt
     4441 tttgcgccac atagatcgta ccgtcaccgg ggaaatcccg gcccaggacc ttcgaaatga
     4501 aggaggacac gagcataccg tgagcgatcc gtctgccgaa ttgggtgctt ttggcgaact
     4561 cctcgtcaag gtggacaggg ttcatatcgc cggtcaactc ggcaaaggct ctcacacgct
     4621 cttctgaaac gaaaaaggtt tgtcgattga tcattccgat accgatatcc gaaaaatcca
     4681 tagtctcccc cttagttact cgtccgattg tgagatcggt cgatgatacc cttaatacta
     4741 ccggtccgga tgaaaaaagt caagggcgaa tgccaaccgc gagtatgccg gaacaggcgt
     4801 gtcgatttcg aacgggtttt ggtgtgggaa taataccgag gagcaacaaa tcgggaacgc
     4861 cgaactttag ttcggcacgt ttttttgctt ttcagtaagc tctcgaaagc ccttaaacgc
     4921 ttacaccgcg atgcccgtgc gaggttatcg cggggtaagt ggttttacga ccccgcgatc
     4981 ccccctttgc ttttggccgc gataggccca gaaaaaagcg tatcgcgccg tcgcgaaaag
     5041 aaggggcgct tacgccgcga taagtagacc cataccgggt tttgtttttt taccagatcg
     5101 tgtataattc ttgtaagaaa ctatagagta gggagtgatc gatgtgaaaa gaacgatagg
     5161 tttggcgatc cttctgtttg tatttaccct cttcttctac ggttgcgcgt atatcgatat
     5221 cggacccaca atagaagccg gttttcgaca tttcaactcc ggggacttac gaacagcgga
     5281 aaactgcttt cgagaagcgt taaggagaaa cccgacgcga cctcaagcta acctcggcct
     5341 ctctctggtc acgctattcc gaggaaatca acgcctgatc tccattttca agagaatcgg
     5401 tcccatagac tatgcgctgc caggcgccct tcgcgcgaac gcgaaaagcg ctctaaaggc
     5461 tgaattacag aagaggttac tcaattggtt ctttaaaacg agggtcctac gcgcggaaga
     5521 cgaccttgat ctctcctgga tacgatggtg gatacaagag gggcaacgtg agatcgccga
     5581 aaccgtcgcg ctattggaag aggccaaatc gtgccttgag cgggcgttgg cttctcctga
     5641 accggtcgat atcttcccga atcatctcga ttggaatcaa aacggcgtct tagaacctga
     5701 aacgcctttg ctgttctctt ggtttgcaca gggcgcgcca gtttggaaga tcatcgcaga
     5761 agccttaatc ggtaaaaatg agctccctcc ggacgaaaag atcagccggt tcttcgatcc
     5821 ctcctcgggc gacgcgtggt ttgacaaaga actgttcgat ctgattcgat ccggagaaat
     5881 cgatgagctg gacctagacg cgtgggaacc ggttttcgac gaggaggacg ttctgacgat
     5941 cggttctacg gagatgcgct ggctatattt attggtgacg ggagaactga gcgccttgga
     6001 accgctcgtt atctaccaac tcgatttcgg aagcgccctc caagattttt tgaaccgatt
     6061 ctcaaaaatt aaccgagaag aggggataga gtccgcgtac gcctatgccg cggattttct
     6121 cgacgcaaac cacgacggaa aaatctccaa cttagaatgg tgcgcgctgt tcgatgaggg
     6181 gtttttggcc ttccgaccag aagaagaaaa tggtggcacg cacgccgtgg agaactggaa
     6241 aggggcaatc gtcgggtttt gcgaaacgat gatctggatg atcgagaacg atcaactgcc
     6301 attcctcgac gacccggagg aagaagggcc gttcggcctg aacctcgcgc gcttggacac
     6361 cggctccccg ttgtccgaac ggcttttggc gatactgcga gaaacgatcg actacgtctc
     6421 cgatccaacg aaagctgtaa cacttttttc aatagagagc gaagagcccc tgttctcccg
     6481 cgtacggggg ttcttggatc taaggggaat cgacagccgt ttcatcgatg aacttcacgt
     6541 attggcctac cccgggatgt atttcgccca cccagaaaac ttttccgact tgaaagcctt
     6601 cttcccacag ctctattatc aatggataga ccggactttc ccgataccgg agttgttcct
     6661 cttcgaagac cccacctttg gcgggttgtt gcaggcggac atcaacgggc atccctggaa
     6721 tggcgtattt tctcagatat ttttcttgat cacgaaacga tagaggagta ccgtgaaggc
     6781 gaacccaaaa accaacgcga tccgttttct ggaacggcat cgcgttgatt ttgaaagtcg
     6841 ggtttacgaa gtggacgaat cggatctcag cgccgtatct gtagcccaaa agctggggat
     6901 tcgggtggag caaaccgtca agacgattgt gctcaagggg aaccgttccg gggttttcgt
     6961 ttgcctgctt caaggtcacc gcgacgtcga catcaaacgc ttggcctcat tcgttccgga
     7021 tacgatgatc ggcaccgttg acgcgggacg cctgttgtct ctgaccgggt acgttcgggg
     7081 tggggtatct ccgttcggga tgaccaaaac ctatccgttc tttctggacc ggccggtctt
     7141 acgagaagag tggatcagtt gcagcgccgg gaagaaaggg atacaaattt ggatgcggag
     7201 ctccgatctc gccgcgttga ccggtgcgac gatcttggat ttttcgaaag atcagcaacc
     7261 tcacatcaaa ggctgataat tccacagcgt gaatcgggcg cgcctttggt cgaaccacct
     7321 ttttggatag aaaaagacgc tcaccgttcg gatgcccaag gcatcgaaga actgatgccg
     7381 tttgttttcg accgtcacga tatgcgggac ctttcgtgcg aaagccctga catcggcggt
     7441 tttgggaaga tagaagactt gttcggaaac acccttgcgg tatttccaag tcgcccccac
     7501 gtccaaggca taggcccgct ccttttgtaa ataaaccaat ccgggttggt gcgaagcgat
     7561 cgtatagagc gaggaaaaaa agccgaacag cgtcgtacga gccatctcgg taagagccgc
     7621 cagggggttc gggaccaacg gtaaaagact ctcggaaaac cctaaatcga aggcgtggtc
     7681 ggggtaagga agtctcctcg cgtcacccag atcaaatcga acacggggat cagattccgg
     7741 agtttcatac aagtccttcg ctttcgaaat aagaaaacga ctggtgtcga ggccttgata
     7801 acggatcccc tcttgcgcca atttatcctt cagaaaagag taattcgcgc cgcatccgca
     7861 accccagtcg atcagcgacg tcgggtgaag gtgatcgatc gcgtcgagaa tggcggcgat
     7921 ctgttctctc cgatagacac ggcgcttttc gggaacggtt tggtcatagc gcacgtcgaa
     7981 ttgtatagag accggttgag tttcgtagct ctgattaccc gcgttcggtt cgaggaggcg
     8041 atggatcctg aaaaggtcgc ctgggcgata gggaatccac tcgttcagca agcctttttt
     8101 tcggtagaac cggtggagtt cgtggaaggg aaccggatcc gcgataaggt gaacgggagc
     8161 cgatcctggt cctttgaggt cgtcgacgac ccgccggacc tctgaaacga gggcgcgatc
     8221 cgtgaaaaaa agcgttaacg aggtttcgtc tcctcttcgg aaccagtaac cgtaatcgga
     8281 gcagatttgc cgggtcgttt ccaaaccggc tacggtcacg ttgagataga agtccataga
     8341 gccgggttaa agcaggattc tttccggttc caaagccatg ccggacaaga tgatccgtat
     8401 gatgcgttcc agcgtgatgt tctcttccaa agcgcccata cccatcattt gcgcgaggag
     8461 tttggccgtc gcttttgtcg atatgtcgga gggcagggcc tttttttctt tcccttcctc
     8521 gatcagttct tcgatttttt gcaagaaacg gttgtacctg agccgaaacc ttccccactc
     8581 gtctccgtcc tgcggcccgt ccataattcg aacccgatcg atctctatca gtttcatcac
     8641 ttctcgacgg ctcatcacaa aatcgatgta accgcggaag agttttttca acttccggat
     8701 atagacgctc tcgtccgcca gttctctaaa gagcgtattc tcaagctcgt cgatcgattc
     8761 gttccaggct tctaaaaaca acgattcttt tgaagggtag tagtaaaaaa tcagcgcttt
     8821 tctcacgccg gctttttccg cgatgtcctc taaggttgaa gcttcgtagc ctttgcttgc
     8881 gaaaaccgct tttgccgctt gtaatatgtc acgtttggtg tctctcttgg acaaccgtat
     8941 ccctccaatc cgaagcgtcg taccgcttct tattctatca cgttccgcga aggaattatt
     9001 tgaccgtttg gtaaattatt tgtaagaaca attcagttct tgaactaaat caactccctt
     9061 ctgggattaa cagaaggtat gccgccggtc ggctccacgc tttcgcgcgg cgagtttttc
     9121 tggactgcct ttttacccgc gatgacccaa acaggcatcg cagcgtaagc gcctcttctt
     9181 cttgcgacgg cgcgaaacgt ttttttccgg acccatcgcg gaactttaga aaaaaacaaa
     9241 agcgggatcg cggggtcgta aaaaccactt accccgcgat gacccacaca gacatcgcgg
     9301 cgtgagcgcc tcttcttctt tcgatggcgc gaaacgtttt tttccggacc catcgcggcg
     9361 taagcgtttt ttctctatgc gacggcgcga tactttgtgg gttgggaacg ccaactttta
     9421 agttggcata tctaaaaaat ctcccacgga gaccacggag gtcacaaaga agagaggtgt
     9481 ttaggagagt caacacccag gtttatgttt cctctgtgct ctctgcgtac tctgtgcgaa
     9541 accatctttt ctttgaacct gccgaactaa agttcagcgt tcccattttg gcgcaccccg
     9601 atattatacc cacacctcac aaaagtccgc tcgcgatttg tgttataatc ttcggaggta
     9661 ccgtaccttt catactctgt acggaggtgt ggcgttggag atctggaaaa acaccgtcga
     9721 gtcgttggtt cgcgggattc gggataagag ctggagcccg agagatattt tagaagcttt
     9781 ctataagcac gcgtgggccg ccgactcgag gatactggcc tttattcgga tgccgaaact
     9841 ttcggagatt agtattccgg atgccctgga cagtttgcct ttgcctttcg cggtaaaaga
     9901 caatattatg gtggagggct ttcagaccac ttgtgggtcc tacgccctat ggaactacaa
     9961 agccggttac gatgcgacgg ccatcgcgcg cttgaaagcc agcggtgcgg tcttctttgg
    10021 gaaaacaaat atggatgagt tcgctatggg ttcttcaacg aaatactccg ctttttttac
    10081 aacgaaaaac ccttgggact actatcgtat tccgggtggg agcagcgggg gctccgcggc
    10141 ggcggtagcg gccgggtgtg ttcctttcgc gctcggatcc gacaccggcg gttcaaaccg
    10201 cctgcccgcc gcccactgtg ggattgtcgg atacaaaccg agttacgggt tggtttcgcg
    10261 ttacgggctc gtctctttag cctcttctct cgaccaaatc ggacctctct gccgttcggt
    10321 gcgagatgcc gcgtgtatcg gcaatatcct catcggaaaa gacgaaaagg acgctaccac
    10381 cgtcgaccgt ccgatggatt tattgaaagg tatcgaaaac tcgattgcgg ggaaacgttg
    10441 cgccgtggta aggcagtcag ttcccgaaac gatcgacccg cacgtgcggc gtgcgttttg
    10501 ggaggcagtc gagatattta aacaaaatcg atgcgttgtg gaagagatcg actttcctga
    10561 ggagaggtac gcgatcgcca cctaccacat cctcgtgccc tcggaagctt cctccaatct
    10621 cgccagattc gacggtgtac gttacggctt caggataccg gaagaaagct tgacagatat
    10681 gatggccgaa tcccggcagt atggcttcgg tgaagaggtg aagcgaagga tgctattggg
    10741 cgccttctac ctcggagagg ctcaatatga ctcgcactat caaaaagcga tgaaggttcg
    10801 acggctcatc tcacagagag tccaccagat atttcaagat tacgatgtgc tcctctcgcc
    10861 cacatcgctt ttctcaccct ttcggataac ggagaacacc gatccgttag ctttttacct
    10921 ttcggataaa aacacggtct tagccaacct cgttggactg cccgccctgt cgatcccgat
    10981 gggattcgct aacggaatgc cgtgcggctt acatttgcaa tgcaagcctt tcgccgacga
    11041 gatgctgttc aatatcgcgc gcgcctacga gaagagcgca gggtttttcg aggacgatca
    11101 ctatcccttc ccatttcagg aggagcgctt atggctataa aacctcagat cggcttggaa
    11161 atccacgcgc aattgctgac gaagacgaaa gcgttctgca gctgttcgtc cgagtccttc
    11221 gatgcgcctc cgaacaccca catatgcgac ggttgcaccg gtcagcccgg aactttacca
    11281 aaattgaatg ccacggtcgt ggagttcgcg gtgaaggcgg cgctcgcgtt aaattgccac
    11341 atcaatgaaa ggagctcttt cgatcgaaag aattactttt atcccgatct ggtcaaaggc
    11401 ttccagatca cccaatactt ccgcccgatc gcagaaaacg gaagtctgtt gatccacgcc
    11461 gacggacgca agaaaaaggt acgcatacgc agagttcata tagaagagga tacgggaaag
    11521 cttctccaca gccccgccga ccgaccggaa tgtgcgttta ttgactacaa ccgttgcggt
    11581 atcgctttgc ttgaggtcgt cacagaacct gatctggata cgcctaaaga ggcccacgcc
    11641 tttatggaga ccttgcggaa tacctttcga accctcggga tttgtagcgg agatatggaa
    11701 aagggcgcgc tacgctgcga cgcaaatatc tcggtccgag acacggaccg tggcttggta
    11761 acggctcgtg tggaaataaa aaacgtcaac tccttcaggt tcgttgaaaa agctctggaa
    11821 tacgagttcg aacggctcaa ggcggcgctc ttatccggag agaagacggt ctcggaaacg
    11881 cgcggatggg accaaggaag ccgttcgacc gtatcgcttc gaagtaaagg agaagagaac
    11941 acctaccgtc attttcccga acccgactta cccgatatcg ttctagagcg cgcctggatc
    12001 gaccgcatcc gcacaacgat accggaactt cctatggaaa agcaagatcg actgatggag
    12061 caatacggct tgacgatggc cgaagcgaag actttcctcg acgagcctga actggaaacc
    12121 tttttcgtcg cagcggtggg acaaggacta cccgtgaaag cggtcaagaa ttggctgctc
    12181 ggggagatac agaaaaccct caaagagagg gcgcttacga tcgatcggac aaccttgacc
    12241 gtggcacgtc tggcggagat catcgaatgg attgccacgg gcaaaacgac cacccacatc
    12301 gcgaaaacac tgatcccgga tctattggag aacgatatct ccctagagca gctcctctct
    12361 gaaaaaggga tttcgcgaat caacgacagc gcctacgtga tcgaagtttc caaaaaagtg
    12421 gtggaagaac acccggagca agcggaagcg tatcggtttg gaaagacggg attgttgaaa
    12481 tactttattg gtctggtcat gaaagaaacg aaaggacagt gcgatacctc cctggttcaa
    12541 gagacgatta aagcgctgtt ggaggggaat aacattgagg gatagcgatg ccggtttttg
    12601 gaatcgggtt cccgcctctt cggaatccga aggaaaaggg caagcggcgg aagaatccga
    12661 cctcttgcgg atagagcgga agatagaccg gattcaatgt tttttgaatg acaactatcg
    12721 ggagctggaa gaaagcttaa aaaagcagaa gagttacttc aacgcgttgg agattatgtt
    12781 ttatattttg ttgggtatca gcctagccca attcattttt ttaatgatac gataaaggac
    12841 tattgcgatg attttacccg ttgaactcac tatgatccga ttttctgaag agtattccgt
    12901 tattttgaag gaaatacgag aaaagaacca tttaacaaga aaagtgccaa aatctctctg
    12961 gaaagtgttg gatttcgaat tcgccactga aaaggacgca gtggtcatca cgggcggttt
    13021 tgcggaacga gaaatatacg tgatcgcgat cgtctcgttc aaagaccgcg ccataagttt
    13081 gcattgtagc tgcgggtacg acacgcggat agaaaggctc tgccctcatc tccttttcct
    13141 cttggaggag gctgggagac aatcgagcaa agtcgaacag ctctatgagg agcataagag
    13201 gattcaaatg ttcgaatccg gttatcgtaa atacctggga aacaaagagc aactcactca
    13261 tctcctctct ttctttcata cggaacaacg gaattccagc gaaaagggag caaagtccga
    13321 agaggagaaa tttgtcgtgc tcctgaaccc gtgtaacagg gagctgcttg ctagagtcgc
    13381 ccggcggaaa agaaacgcaa aagggaaata cctcgctacc ggtcaaaaac tcgtgagtta
    13441 tcaacggttg tacaagaggg acgatccgtt ctttgcgcgc gttttgtcct gtatgagtta
    13501 tgttcctccc tcgactgatt cctatcataa tgaattttct ttagaaatca cagattttca
    13561 aaggttcgta gaggcttttc gggatgatga ttgcgtttac attaactatc tgaatgaacc
    13621 ggctgttttc ggtcccacgc ttacgcttca tctgaagccg aaacgctcgg ggatcaaagc
    13681 gccggatcag caactgttgg aattccggtt gatcaacgaa gaaaacagga tcgagtatcc
    13741 attgaaagct acgaaaacaa aagactattt tttgcccgct ttcgcttatt atggaaagct
    13801 gggtttgaaa ttggcgatca tcaccaacaa gatttttccg gtcgtcacct cattggacgc
    13861 agaaacgact tttcgtctcc aggaactgct gaaagactac gattggggag cagagaaaga
    13921 agaaaataag ggatctctgc agcgactact gttcttgccc ctactctctt tgcaggtcaa
    13981 cgtcgcgccc cagctgttcg gaaatggaat ggcctatacc agcgaaaagc cgaaggctct
    14041 atggcagatc caaagttatc attggaacta tcgatttcag ctgttcttca agtacggaga
    14101 tcgccgtttg tcggccgaac aacccgaaag tatcattatt tccgaaacca ccggccaaat
    14161 ctttaagaga gatttggagt tcgaggctca acacattcaa actattcgga atatgctcag
    14221 ccacgagacc tgtctagacg cagagagcct actttacgac ttacacttgg agagcgtttt
    14281 gcgcctagtc aacgaggagt tcccaaagta cgaaaaagaa gtggtgttgg agtgcgacac
    14341 tcggctcttt tctcgcgggc gggtcaagcc gatcgtcaaa acgtttttct catatggtac
    14401 cgactggttt gatctggcgt taagcgggga actgaacggc gaacccatcg ccgctgagga
    14461 tctggatcgt cttttggcca acccctctca atatcttcag atcaacgggg aatacttctc
    14521 cgtagatcct cacgaggcgg ggaaactcga aacactgaga tcacggttaa gccagctgaa
    14581 agacgggttg cgtatcccga aggcagactt cgagacctta caaacactta gggaggtgac
    14641 gacaccctct ttcgacgaga gaaccgaacg gtttctttcg gcgatcgata agttcactca
    14701 aatacaggag tatccggtcc catcggattt cggcgcgtca ctacgcccgt atcaaattgc
    14761 ggggtacaac tggctgaggt ttttggagga atacggtctt tcgggaatac tggccgatga
    14821 tatggggttg ggtaaaaccg ttcaggcgct cgcgatgcta cacgatttta aacgtcgata
    14881 cggacggcta tccgcgctcg tcgtcgcgcc gaagtcgttg ctgagaaact gggaggccga
    14941 gaccaaccgt ttctcgccgg atctcacgac cctgatctat catgggtacg gtcggcaaaa
    15001 ccaagatatc tcgaagagca cttctgattt gatcttgacg acctacgcca cgttgcgcaa
    15061 cgacatccgc ctatttaccc ccaaaacctt caattacgtc atcctcgacg aggctcaaaa
    15121 tataaagaat aaaaacacgc ggactttcaa agaattgacg aaagtgcaag cggagaaccg
    15181 gctcgccctt tccggcacgc cgatagaaaa tgggttgtca gacgtgaagt ccattttcga
    15241 ctttttaatg cccggatttc ttggtgctga caaactcttt ttttccacct acaacgagaa
    15301 cttcgaagag ctaggaaaga agatcgcccc tttgatcctc aggagaaaga aagaagacgt
    15361 tttagccgaa ctgcctgaaa aaaccattga aaacctgtat gtcgacatga cgaaatctca
    15421 atccgatatt tacgatacct acttcaaagc ggcacagtcc gaggcgcttt cgataatgcg
    15481 cgacgaagcc ggttttcagc gaagccgctt tctaatattg acccttttac tgcgcttgcg
    15541 tcagatctgt tgccatcccc gcttgacgaa agaggaattg tctggcaaag ggctgtccgg
    15601 taagttggaa gctttaaggg agttggtcga cagtatttgc gccgaaaatc acaaagtttt
    15661 agttttttca caattttcga agatgctgga gatcattcag gaggacctct ccgactacaa
    15721 agatcaaatc ctgacgatga ccgggaaaac gcaaaaccgc caagagatca tcgacgcttt
    15781 caaagaaaac cccgaaactc ggatattctt gatgactttg aaggtcggcg gtgtcggcct
    15841 caacctcacg gaagcggact acgtcatcat ctttgacccg tggtggaatc cggcctcgga
    15901 ggctcaagcc atcgatcgca cccatcgaat cggacagaac aaaccggtgt tcgtctatcg
    15961 actgatcgcg aaagactcga tcgaagaaaa ggtgctggag ctacagaaac gcaagcagag
    16021 tgtggctgac ggggtcttga gcggtagtga acgcgatggc gggtctttcg gaaaagagga
    16081 gttaacttac ctgttctctt aagggtccta ccttttcagc ctgccggaaa ggctatccaa
    16141 gagcaaatgg tttaaaaacc cgagcagggc gcacataaag atgaaaaaca accgcgacaa
    16201 cgccgctttc tccgtcgata ggttttcaaa caaataccag tataaggccc cgagggccaa
    16261 ggcgtaaacc aaaccgaaca agaaggtgtg gatcggccct ctgtgaaccg gaagcaattt
    16321 gaagcacacg aaagaaacga gccctccccc cgccagcgat agaacgaaca gcgcttcttc
    16381 gatcactgga tcgaagaccg ggggtaggag gtaatacccg tgcgtaaaca gggcgcggaa
    16441 aactg
//
LOCUS       DAOPXN010000035        16216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3668_length_16216_cov_5.208589, whole genome shotgun sequence.
ACCESSION   DAOPXN010000035 DAOPXN010000000
VERSION     DAOPXN010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16216
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3668_length_16216_cov_5.208589"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1140
                     /locus_tag="PKM49_03250"
     CDS             235..1140
                     /locus_tag="PKM49_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY81866.1"
                     /translation="MKRLSLWLLWVSLALTVSYANTASAAIFTSLEREVSFCVRGQTV
                     RGVLTVPAEKDASSPAILLLHGFLGHQNDLRVYGTTDGLYPIVARCFAQAGLVTLRFD
                     FRGSGESDGNWEETTFSRQILDAQAAVDFLIELGNVNPEKIGAIGLSQGGLVAACLGA
                     RDDRIGSIALWSPVAIPIHTYGHLLGFQTLEKALLVDETLTSPISWGGTTRLNSAFFR
                     EMFEIDPLAEIASYQNPLLVAVGIEDPIVYPQPESGELFLTYHPGEEALLVFSCDHIF
                     NLFSERAILDALIDETLLWFLRTLE"
     gene            1412..3706
                     /locus_tag="PKM49_03255"
     CDS             1412..3706
                     /locus_tag="PKM49_03255"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNY81867.1"
                     /translation="MKKKRDLNVIFTVFILLTTALFGIYVVKNLYFDSTFYAFLDRTS
                     PTLIASEAIAKKYGDTTTIMVLLPFDPDRAEESLREVDNVVRALEKVPFVQSVESLFN
                     AQKNGGLLLGAPFIRFQPYVTVTEDSENPGHRKYAFDRGILQDRAYSGILISEDGRSV
                     TVMLRKRAEMRVSAIELSNRLQSEIRSVTSLPFKLIGEDIVEADLFDSVRVLAFVYTP
                     LIGFVVLGIFLLKFRSLFLTLLTLVPPLLSSIWVYFFMLVFHRNINSLTVLIPTFIVI
                     IGSAYGMHYLSRFLEVRKETERRSGGGEGGRISSASLSATVKQASREEWMPILFSALT
                     TMAGFASNIFLQMEAFRDMGIFASLGIFLCAVFTMVVTPSFLYFYRKSPAQKQESSIR
                     ARSTRESRFGRYLKWAFIGFTAIGIGLSPFLIPQVSLEMDSYRYFKESSTLRQSANQL
                     KADFGWVSNMYLVFETPEGRPLSIQSEGAQKIKTFLDQVERIPQIANQMSIVKMSESL
                     KIPMPLLVSATRMSAQGKEMTRPFITEDSFRVMLFLTSNDSAAAKEVKSGIESAWEDA
                     ELANDYTIRLTGAPLVWAELSALVVSNQVESTLIAFGMILLLLFISFRSIKTALFSSI
                     PIGITVVFNFLFMSIFKISLDIPTAIVAGMLQGLVIDYAIHFMFWYKQTQDVEDAFHK
                     TASPILFNGMSLIGCFAVLLLSPMMVYVKIAWLMVLGITVGVLTTLVFLPALLRWRLP
                     LTFKPVWNRRKDASVQNAREHSID"
     gene            3666..4406
                     /locus_tag="PKM49_03260"
     CDS             3666..4406
                     /locus_tag="PKM49_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81868.1"
                     /translation="MQAFKMLVSILLISASASVCWGYSEAAWTLEAAVERFTVLNSHR
                     DRDAMEEFLRQLQNQVTLRSDLYPVLCTALFRYADWGIVSDAERNRTYHRAIEAGEKA
                     VRYSPDNVLSHYAYALSCGRIAEREGGLGALDLLKKFETHLQQVLSKDPEYYLAYYAL
                     AMRYRDAPWPMKDEKKAEEYYRKTIWIEPGFFNPYYDLSLLLIRQNRKAEAKELLLIV
                     ANLPPHPDWIAESEEIGRLSNALYASLR"
     gene            complement(4423..5730)
                     /locus_tag="PKM49_03265"
     CDS             complement(4423..5730)
                     /locus_tag="PKM49_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNY81869.1"
                     /translation="MRSLQTKISLWYLISFSILFLVFIIVVFSLFRHVIYDHFDNRLR
                     SLAEEFSRFFELNDPKDNQLLSLAGKEEIVLEVYQKKRLYLSFQVRPGHFAEWVSKET
                     VGYFSTDVYRCYIERIPDTDLVIIVGIDQRFYFGDLPVLIFEIVGVVAACMLSIYFVG
                     KVISKRLLSPLEIIGEELLDTVNRNIEGKRIAVPVTGKEIGNLVFAINLSLGKLEKYL
                     KELKGFSAYLAHELRTPLTLVKMTLQNLKTSEESLSFRQSIEEADQEVEEAIQFLNDL
                     LLLSNLEKGIIERFQDFDLGEVILQNFEKASKLFPKKKIEVDVSAVTVYRGAAQLISH
                     AVYILLENACKHGEEDTVSIRLYQSVRLFGEQRPVNVIRIVSVGKKVHWDELKQMPIL
                     KGHGIGLNLCRRIAQIHNGTLSYTFQETYQKELVKNAFELILP"
     gene            complement(5732..6406)
                     /locus_tag="PKM49_03270"
     CDS             complement(5732..6406)
                     /locus_tag="PKM49_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY81870.1"
                     /translation="MRILVVEDHQKIAESIKTGLEKEGFEVVCAYDGESALDALESLS
                     FDCVLLDIMLPDISGFELCRYIREDLANRVPIIMLTALDSLENKVEGFNCGADDYLPK
                     PFYFKELDARIRALIRRNINQTADIIRYGPFSYDPYKRLLRINEVEVQMSKRELELME
                     LFLKNPQKVLSRDFIKGEIWGESEEGKSNIVDVYILYLRKRLRPYGLEKSIKTYPQVG
                     YILDIA"
     gene            complement(6437..7009)
                     /locus_tag="PKM49_03275"
     CDS             complement(6437..7009)
                     /locus_tag="PKM49_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81871.1"
                     /translation="MPAFCICKKTCVRRIARRRYLLLLACSLIFCAGFSWPSIQDGGG
                     CLGDPLTLSPTQSGTLTIFSIMDSLVSVSLENGEFWISIVYSGENQSWTCMTNLCRYS
                     SSADGSLERTSPHPTSIKEEPKVSLFDVLTLARLQTSDAILAVYLCNDRERDYWRVLT
                     GSEVYSIDSEKPIVFKREKLIQAPEPNAND"
     gene            complement(6987..7439)
                     /locus_tag="PKM49_03280"
     CDS             complement(6987..7439)
                     /locus_tag="PKM49_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81872.1"
                     /translation="MKMTPEMTEAQERMAPGVITAQGFLGHDTRNVAEIIAEDERYFA
                     LREIPIERLVERMRELQEAGSAGLGETISVEPYWEVRVNEARGVLPCPFSDGVYGKIN
                     TIVIDRQSKEEIVYSDLTIHLIEKHHFFQGVGSPFRHEPEKYARILHL"
     gene            complement(7463..8572)
                     /locus_tag="PKM49_03285"
     CDS             complement(7463..8572)
                     /locus_tag="PKM49_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HNY81873.1"
                     /translation="MWTAPIIISAISAVLAFAIAIIDGIVNNYADVTISINEGQKTLS
                     VKGGSPLLSSLATKGVFVPSACGGKGSCGACKVKVLSDIGPHLPTELPFMSPEEIKDN
                     IRLSCQVKLKSDIRIRLPEELFSVRQLNCTVESLKDVTYDIKELRIRLPKNATIDFKA
                     GQYLQLVIPPYQEIKESAQRAYSISSKPSDKNSLEFLIRLVPNGIATTYVHRELKEKQ
                     PISVIGPFGDFCLRDTDADMFCVAGGSGMAPIRSILLDMYEKKINHRTVWYFFGARSR
                     RDLFYLNEFRELERLWPCFHFVPALSEPLPDDRWEGETGLITQVLDTYMKKNPDPSKS
                     KEGYLCGSPGMINACVKVMIANGIPENEIYFDKFA"
     gene            complement(8595..9188)
                     /locus_tag="PKM49_03290"
     CDS             complement(8595..9188)
                     /locus_tag="PKM49_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNY81874.1"
                     /translation="MTPDVHPLTLLIASVFTNNILLSLFLGMCSFISISKDLKASNGL
                     GMAVTLVLTITTLINWMVYHWVIVPFELDYLRYIVFIIVIAAIVQVLEMVIERVSPTL
                     YRGLGIFLPLITVNCAIMGVSLFMQLRNYDLLQSAFFGAGSGLGWWLAIVLLAAIRQR
                     IEKNPIPAGLKGAGITLITIGIIGMAFLGFAGMLKVQ"
     gene            complement(9193..9807)
                     /locus_tag="PKM49_03295"
     CDS             complement(9193..9807)
                     /locus_tag="PKM49_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HNY81875.1"
                     /translation="MSLNAATNTTRILKDNLFRNNPVASQIIGICSALAVTNNLVNTF
                     IMGVGVALSTAFSSFTLSLMKAWIPQKVRMIIQVLVLSFYVILLDILLRAFIPDIAKA
                     LGPYIGLIITNCILMGRAEAFAQANPPGAAFWDGLTSGIGYLMVLMIVAFFRELLGFG
                     TLFGVPVLPDQFIPWTIMVMAPSAFFVLAIYIWIHNTLLAKKRK"
     gene            complement(9804..10439)
                     /locus_tag="PKM49_03300"
     CDS             complement(9804..10439)
                     /locus_tag="PKM49_03300"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNY81876.1"
                     /translation="MKLNLLEYKDHKAYLVGFAVLVTFIFVLVLSFVNELTKEQVKKN
                     QELFLIRAVLESMQIPYQNDQEALTIYRGRIGVKEVRGKRIFFTNPPEDKKYAILFSG
                     NGLWSIIQGIIGVDSQFERLTGIAFVEQNETPGLGGRIEEPWFKGQFQGEKLVNGTVA
                     FRTGQADPGDRDKDNGSVDAITGATSTSKSLETILKKALKDLEERVKEGGL"
     gene            complement(10436..11310)
                     /locus_tag="PKM49_03305"
                     /pseudo
     CDS             complement(10436..11310)
                     /locus_tag="PKM49_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
     gene            11638..13791
                     /locus_tag="PKM49_03310"
     CDS             11638..13791
                     /locus_tag="PKM49_03310"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNY81877.1"
                     /translation="MERLRTVNLPVEMRRVFGFLYKNYTEEELRNVLEAYEFAKRQHA
                     EQYRKSGEPYINHPLKVAQILAEMKLDTVSIKAGLLHDVVEDTGTSLQEIEQRFGKEV
                     ARIVDGVTKIDRLKIDQKNFDQKAETVLKMFFAMSQDIRVILVKLADRLHNMRTLEFQ
                     SPETQKQKAKETLQIFTPIAHRLGIYTIKWQLEDLAFKYLYPEEYTHITSLVDSKRAD
                     RELITQEYLSVLQLTLARNGIQSEISGREKHFYSIWKKMNERGKNFDEIYDLIAIRVI
                     TNTDKECYYVLGLVHNLWKPIPGRVKDYIATPKSNGYRSLHTTVITHRGEPLEIQIRD
                     KEMHNEAEFGLAAHWIYKEGKMQKHQQTWLKKLQEWHQDYEQGVTGLNEFQKEIQLEE
                     VYIFTPKGELKHMQKGATTIDFAYSVHTEVGHHYAGAKVNGKIVPIGYELQNGDVVEV
                     IVNKNNPGPSLDWLKYAKSSSTRAKIRRFFKEKDAQVLIEEGKNLLRQVAKKLQRSME
                     DLLESDELKQYLGIDRQVSDRDILLRIGEKEHNAEDIVVLFQPQPIEPPPVMGPPDKK
                     QVQSTTIVVDGQLGIEVKMAKCCLPVPGDPIVGVISKKGITIHQKDCPNAENIMKGEL
                     VSVQWGNTDQERFMTHVILETNDHSGNLLKNICQSAKERNYHITEVNTKLDVIKHMTY
                     RLSIFVKSLTQLEEMLEYWRKQNGIVNVYRTRGEG"
     gene            13788..14231
                     /gene="dtd"
                     /locus_tag="PKM49_03315"
     CDS             13788..14231
                     /gene="dtd"
                     /locus_tag="PKM49_03315"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNY81878.1"
                     /translation="MKTVLQRVKRASVTVDGLLISEIGPGLLVFLGVGESDEIADLEW
                     MAEKIVNLRIFEDENEKMNLSVKDTGGSILLVSQFTLYGDAQKGRRPSFSEAAKPEKA
                     KVFYEACGRRIAELGVPVSMGVFGAMMAIDLLNDGPVTILIDSKR"
     gene            14241..14819
                     /locus_tag="PKM49_03320"
     CDS             14241..14819
                     /locus_tag="PKM49_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNY81879.1"
                     /translation="MKPQRLLLHICCAPDATVAFVKLKERFEVEGLFYNPNIFPEEEY
                     RKRCSAARDLAEAWSFLLHEGPYDWESWKALTRGYENVDEGGERCQLCIAHNLRQTAV
                     FAKALGFGWFSTSLFTSRKKDLRMIESLGYRIGKETSVQFYYEPFRRAGGFDESVRHS
                     RELGLYRQTYCGCPYSAQGANSNGPSGSTRAG"
     gene            14819..15745
                     /locus_tag="PKM49_03325"
     CDS             14819..15745
                     /locus_tag="PKM49_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HNY81880.1"
                     /translation="MKTERQESWKIQVPFHLDYCLNCGQVFRWKKLGNHWFGFIEGHL
                     YRIEYEESSQTIRALRISGDTGRTGADSEGESRRRIERYFGLYDSLSAIRESILTNLR
                     EKGYNWFVGKFDDIFSKSLGLRILRQDVWEVAVSFLLSSQTNIPALERKLDALCRLFP
                     QNEVRYEGESYYRFPKRESLATLCEEDFKRLGFGYRSAWLCEMVRRFRESDFRSIRTE
                     TYDRQLSFLLSFPGVGYKVAHCILLFGYSVMRAFPVDIWISRWIKEVFHVEGAPDRLT
                     SWGQNVFGEYSGYAQEYIFNVYRNLGSDGGEI"
     gene            15742..16161
                     /locus_tag="PKM49_03330"
     CDS             15742..16161
                     /locus_tag="PKM49_03330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY81881.1"
                     /translation="MTERLSIEFNAIIDHVRVIRGALRSFLRSKEINATQVFDTELAL
                     SEAVVNVIKHTYRFEPTQTVYLTILWEPEERACTFLLEDSGKTVSPEKMLSRDLDDLK
                     DHGLGVYIIKSLMDEVTYSPREDGSGNRLTMKKRFRP"
BASE COUNT         3961 a         4072 c         4327 g         3856 t
ORIGIN      
        1 cccagaggga tgcatctacc ttttcatcta tctcgttttt ctctgtgttc gccgtgagct
       61 ctgtgtgaac cccttctttt ctttctaaac catgccgaac taaagttcgg cgttcccaag
      121 ggccgtcccc aaagtgcgct cacaaagtga cccaagcgct tccggaaggt cggattaacg
      181 gatcgtggaa tttggcgtat aatacagtgc aaaatcatcg gaacaggtga tcgtatgaaa
      241 cgcttatctt tgtggttgtt gtgggtatcc ttggcgctga cagtctcata cgccaacacc
      301 gcgagcgcgg cgatttttac ttcgctggag cgggaggtct cgttctgtgt ccgcgggcaa
      361 acggttcggg gggtgctcac ggtgccggcg gaaaaagacg cctcctcacc ggcgatcctc
      421 cttttacacg gtttcttggg tcatcaaaac gatctgcggg tctacgggac gacagacggg
      481 ctctatccga ttgtcgcgag atgcttcgcg caagcgggcc tggtgacctt gcgcttcgat
      541 tttcgcggtt cgggagagag cgatgggaac tgggaagaga cgaccttttc gagacaaatt
      601 ctcgacgctc aagcggccgt cgattttttg atcgaattag ggaacgtaaa cccggagaag
      661 attggggcga taggcttgag ccagggaggg ctggtggcgg cttgcttggg tgcccgcgat
      721 gaccgaatcg gaagcatcgc gctgtggtcg ccggtggcca tcccgattca cacctacgga
      781 cacctactcg gatttcaaac cttggagaag gcgctcctcg tcgatgaaac cttgacgtct
      841 ccgatctctt ggggcgggac gacccggttg aacagcgcct tttttagaga gatgttcgag
      901 atagacccgc tcgcggagat cgcatcctat caaaacccgt tgctcgtggc cgttggaata
      961 gaggacccga tcgtttaccc ccaacccgaa agcggcgagc tcttcctgac ctatcaccct
     1021 ggagaggaag ccctcttggt gttttcctgc gaccatatct tcaacctgtt ctccgaacgg
     1081 gcgatcttgg acgcgctgat agacgaaacc ttgctgtggt ttctgcgaac cctagagtag
     1141 cttacggtta ccgagcccac cacatcccgg aggggcggaa acggttcggt ttagcgcctt
     1201 gggcgggtcc tcggatacga agccccgatc ttcggtcttc tggaaaatta gtataaaaaa
     1261 tcgatagttc aaattttgaa ataaatcttc gcttttctac gtcgtaggaa gaaacggatt
     1321 ggcaccgttt ttcctcggcg agcacgaacg aaacaataaa atttgccaac gcaaaggcta
     1381 tatttttggc cgaagagaga ggagcgccac tttgaaaaag aagagagacc tcaacgtgat
     1441 ctttaccgtc tttattctcc ttacgacggc cttattcggt atttacgtgg taaaaaacct
     1501 gtacttcgac tccaccttct acgccttcct agaccgaacg agcccgaccc tgatcgccag
     1561 cgaggccatc gcgaaaaaat acggggatac gacgacgatt atggtgcttc tccctttcga
     1621 cccggatcgg gcggaagaga gtttgcggga ggtcgacaac gtggttcgcg ccctagagaa
     1681 ggttcccttc gtccagtcgg tcgaatcctt attcaacgcc cagaagaacg gggggctttt
     1741 gttgggtgcg cctttcatac ggtttcaacc ctacgtcacc gtaaccgagg attctgagaa
     1801 tcccgggcac aggaagtacg ctttcgatcg ggggattttg caggataggg cgtactcggg
     1861 tattcttatt tctgaagacg gtcggtccgt caccgtgatg ttgcgtaaac gggcggagat
     1921 gagggtctct gcgatcgaac tgagtaatcg acttcagtcg gagatccgct ccgtaacgtc
     1981 gcttccattc aaactcatcg gcgaagacat cgttgaagcc gatttgttcg attctgtgag
     2041 ggtactggcc ttcgtctaca cgcctttgat cggattcgtc gtgctgggca tttttctgct
     2101 gaaattccgg agcctctttc taaccttgct gacgctggtt ccccctttgc tctcatcgat
     2161 ttgggtgtat ttttttatgc tcgtctttca taggaacatc aacagcttga cggtcctaat
     2221 tccgaccttc atcgtcatca tcggatccgc ttatgggatg cattatcttt ccaggttttt
     2281 ggaggttcgg aaggaaaccg agcggcgttc cggtggtgga gagggggggc gcatttcgtc
     2341 cgcgagtctt tcggcaacgg ttaaacaggc gtcaagagaa gagtggatgc ctatcttatt
     2401 ctccgctttg acgacgatgg cgggttttgc gtccaacatc tttctgcaga tggaagcttt
     2461 ccgtgatatg ggtatcttcg cttctttggg catctttctg tgcgcggttt tcacgatggt
     2521 agtaacgccg tccttcctct acttctatag gaaaagccct gcgcaaaagc aggaatcatc
     2581 catacgcgcc cggtctacac gagagtcccg tttcggtcgg tacctcaaat gggctttcat
     2641 cggtttcacc gcgatcggta tcggactctc cccgttcctc atcccccaag tctctttgga
     2701 gatggattcc taccgttact ttaaggagag ctccacgctc cggcagtcgg ccaaccagct
     2761 caaagccgat tttggctggg tttcgaacat gtatttggtg tttgaaactc ccgaaggaag
     2821 accgctatcg atacaaagcg aaggggcgca aaagatcaaa acctttctgg atcaggtaga
     2881 gagaatccct cagatcgcca atcagatgag catcgtcaaa atgagcgagt cgctgaaaat
     2941 cccgatgccg ctgttggtct ccgcaacccg tatgagcgcg cagggcaagg agatgacgcg
     3001 gcctttcatc actgaggata gttttcgcgt gatgttgttt ttaacctcaa acgacagcgc
     3061 agccgcgaaa gaggtgaagt ccggtatcga aagcgcctgg gaggatgcgg agttggcgaa
     3121 cgattacacg atccgactca ccggcgcgcc tttggtgtgg gccgaactca gcgccttggt
     3181 ggtttccaat caagtggaga gcaccctgat tgcctttggc atgattctgc tcttgctttt
     3241 catcagtttt cgaagcatta aaacggccct gttttcttct attcccatcg gcatcaccgt
     3301 tgtcttcaat tttctattta tgtccatttt caagatctct ttggacatcc ccaccgccat
     3361 cgttgccggg atgttgcagg gcttggtgat cgactacgcg attcacttta tgttctggta
     3421 caaacagacg caagatgtcg aggatgcctt ccataagacc gcctctccca tcctgttcaa
     3481 cgggatgagt ctgatcggtt gtttcgccgt tttattgctc tcgcctatga tggtttatgt
     3541 gaagatcgcg tggcttatgg tgctgggcat caccgtcggt gtcctaacca ctttggtttt
     3601 cttgcccgcg ttgcttcggt ggcgtctccc gttaactttt aaacccgttt ggaataggag
     3661 gaaagatgca agcgttcaaa atgctcgtga gcattctatt gattagcgct tcggcttctg
     3721 tgtgttgggg ttatagcgag gcagcttgga cgttggaagc ggccgttgag cgatttaccg
     3781 tgctgaacag ccaccgggac agagatgcga tggaagagtt tttacggcaa ctgcagaacc
     3841 aagtgactct ccggtcggac ctttaccccg ttttatgcac ggcgctgttt cgatacgccg
     3901 actgggggat cgtctcggat gcagaaagga atcgcaccta tcaccgtgcg atagaggcag
     3961 gagagaaagc ggtccgttac tctcccgata acgtgttgtc acactacgcc tacgcccttt
     4021 cctgtgggcg catcgccgaa agggaaggcg gtttgggcgc gttggatttg ctcaaaaaat
     4081 tcgaaaccca tttacaacaa gtcttgtcga aggacccgga gtattatctg gcctattacg
     4141 cccttgcgat gcggtaccgc gacgccccgt ggccgatgaa agatgaaaaa aaagcggagg
     4201 aatattaccg aaaaacgatc tggatcgaac cgggcttttt taacccttac tatgatctat
     4261 cgctcttgtt gatccgacaa aaccgtaaag ccgaggcgaa ggagctcctc ctcatcgtgg
     4321 ccaaccttcc accccatccg gattggatcg cggaatcgga agagatcgga cgtctctcta
     4381 acgcgctcta cgccagcctc agatagcggt aaagctcggt acttacggga gtatcaattc
     4441 gaaggcgttt ttcaccagct ctttttgata ggtttcctga aaggtatagg ataaggtgcc
     4501 gttatggatt tgcgcgatac ggcgacaaag gttcaggccg atcccgtgcc ctttcaagat
     4561 cggcatctgt tttagttcat cccaatgcac ctttttcccc acgctgacga tccggatgac
     4621 gttcaccggc ctctgctcgc caaagagacg tacgctttgg tataagcgta tcgacaccgt
     4681 atcctcttcg ccgtgtttac acgcgttttc taagaggatg tatactgcgt gcgagatcag
     4741 ctgggccgct ccccggtaga ccgtcacggc agaaacatcg acctcgatct tctttttcgg
     4801 gaagagtttg ctcgcctttt cgaagttttg caagatcacc tctccgaggt caaagtcctg
     4861 aaagcgctcg atgatcccct tttccagatt cgaaagcaac agaaggtcat ttaggaattg
     4921 gatcgcttcc tctacctcct ggtcggcctc ttcgatgctt tgtcgaaaag aaaggctttc
     4981 ctcggaggtt ttcaggttct gaagcgtcat tttcaccaag gtcagcgggg tgcgcagctc
     5041 gtgggcgagg taagccgaaa agcctttgag ctctttcaag tacttctcga gcttaccgag
     5101 ggaaagattg atcgcgaaaa ccaaattgcc gatctccttt ccggtcaccg gaacggcgat
     5161 acgcttgcct tcgatattcc tattgaccgt atccagcagc tcttctccaa tgatctccaa
     5221 cggggataac aatcgcttcg agatcacctt accgacgaaa tagatcgata gcatacaggc
     5281 cgcgaccacg cccacgattt cgaagatcag gaccgggagg tctccgaagt agaagcgctg
     5341 gtcgatccca acgatgatga ccaggtctgt atcaggaatc cgctcgatgt agcaacgata
     5401 gacatcggta gaaaagtatc cgaccgtttc tttcgacacc cattcggcga aatggccggg
     5461 ccgaacctgg aaagaaaggt atagccgctt tttctgatag acttccaaaa cgatctcttc
     5521 ttttccggcc aaagacaaca gctggttatc tttcggatcg ttcagctcga agaaacggga
     5581 aaactcttcc gcaagcgagc gcaaccggtt gtcgaaatga tcgtagatca cgtggcgaaa
     5641 aagagaaaag acgacgatga taaacaccag gaacaagata gagaaactga tgaggtacca
     5701 tagcgagatc ttggtctgga gggaacgcat ttcaagcgat gtctaggata taaccgacct
     5761 ggggataggt cttgatgctt ttttctagcc cgtagggtct caaccgtttg cgcagataaa
     5821 ggatgtaaac gtccacgatg ttactttttc cttcctcact ctctccccag atttctccct
     5881 tgatgaagtc cctggacaac accttttgtg ggttttttag aaacagttcc atcagctcca
     5941 gctctcgttt actcatttga acttccactt cattaatcct caacaaccgt ttatagggat
     6001 cgtaggagaa cgggccgtat cggatgatat ccgcggtttg attgatattc cttcggataa
     6061 gcgcccgtat gcgcgcgtcc aattctttga aatagaacgg tttcggcaag tagtcgtcgg
     6121 ccccgcagtt gaacccttct actttgtttt ctagggagtc cagcgcggtt aacatgatga
     6181 ttggcacacg gttggcgagg tcctcgcgta tatatcggca cagttcgaaa ccggagatgt
     6241 cgggaagcat gatgtccaag agcacacagt cgaaggatag ggattcaagg gcgtccaagg
     6301 ccgattcccc atcgtaggcg caaacgactt caaacccttc tttttcgaga ccggttttaa
     6361 tcgattcggc aatcttttga tggtcctcga cgacgagaat tctcatggtt caccctctct
     6421 ctatccggat tcctccctaa tcgttcgcgt tcggttcagg agcctgtatg agcttctctc
     6481 tcttgaagac gatcggtttt tcagaatcta tcgagtagac ttcggagccc gttaataccc
     6541 gccaatagtc ccgctctcga tcattgcaca gatacaccgc taaaatagcg tccgaagttt
     6601 gcaagcgcgc gagcgtcagc acatcgaaga gagaaacctt tggctcttct ttaatagacg
     6661 taggatgggg gcttgtgcgt tctaaggatc cgtccgccga gctgctgtat cggcaaagat
     6721 tcgtcataca cgtccagctt tggttctcac cggaatagac gatcgaaatc cagaactctc
     6781 cgttttccag tgaaacggaa acgagggagt ccataatcga gaagatcgtt aaagtaccgg
     6841 actgtgtggg cgacagggtg agggggtcgc caaggcagcc gcccccgtct tgtatcgacg
     6901 gccaggaaaa ccccgcgcag aagatcaagg agcaggctaa taacaggaga tagcgtcttc
     6961 tagcgatccg ccgaacgcag gttttcttac aaatgcagaa tgcgggcata tttctctggt
     7021 tcgtgccgga aaggcgaccc aaccccttgg aagaagtggt gtttttctat gagatgtatc
     7081 gtcaggtcac tgtatacgat ctcttctttg ctttgcctgt cgatgacgat ggtattgatc
     7141 ttgccgtaaa ccccgtcaga aaaggggcaa ggcagaacgc ctcgggcttc gttcacacgc
     7201 acttcccaat agggttcgac ggatatcgtt tcgcccaaac ctgcgctacc cgcttcttga
     7261 agttcccgca ttctctccac caaccgttcg atagggattt cgcgtagcgc gaaataacgc
     7321 tcgtcttcgg cgattatttc ggccacattg cgggtatcgt gccctagaaa tccttgagcg
     7381 gtgatcacac cgggcgccat cctttcctgc gcttcggtca tctcgggagt catcttcatt
     7441 tcttccctcc ttatcaaacg cgctaggcga atttatcgaa atagatttcg ttctcaggta
     7501 tgccgttcgc gatcataacc ttcacgcagg cgtttatcat ccccggactg ccgcaaaggt
     7561 atccctcctt tgattttgaa ggatcgggat tctttttcat ataggtatcc agcacctggg
     7621 tgatgaggcc ggtctctcct tcccaacggt catccggaag cggctcggag agagcgggca
     7681 cgaaatggaa acacggccat aagcgctcga gctctctaaa ctcattcagg taaaaaaggt
     7741 ctcgacgact gcgcgccccg aaaaaatacc aaaccgtacg gtggtttatc tttttttcgt
     7801 acatatccag cagaatcgac cgtataggcg ccatcccgga tcccccggcc acgcagaaca
     7861 tatcggcgtc ggtatcccga aggcaaaaat cgccgaaggg gccgataacg ctgatcggtt
     7921 gcttttcttt cagctcgcgg tgcacgtaag tggtcgcgat gccgttcggc acgaggcgga
     7981 tcaagaattc caacgagttt ttatccgagg gtttcgacga aatcgagtaa gctcgctggg
     8041 cgctttcttt gatctcctgg tagggcggta tgacgagctg caggtattga ccggctttga
     8101 agtcgattgt agcgtttttg ggcagccgga tccgaagctc tttaatgtca taggtcacat
     8161 ccttcaggct ctcgaccgtg caattcaact gtcgaacgga gaacaattct tccggcaagc
     8221 ggatccgtat gtcggacttg agcttgacct ggcaagagag gcggatgttg tctttgatct
     8281 cctccgggga cataaagggg agctcggtgg gcagatgggg tccaatatca gatagaacct
     8341 tcactttaca agcgccacaa ctgccttttc ccccgcaggc ggaaggcaca aagacccctt
     8401 ttgtggcgag gctcgacaat agtgggcttc cccctttgac cgagagagtc ttctgcccct
     8461 cgttaattga tatggtgaca tcagcgtaat tgttcacgat cccgtcgatg atggcgatcg
     8521 cgaaggccaa tacggcgctg atcgcgctaa tgataatcgg agcggtccac atagctttcc
     8581 ccctttcgat gaccttactg tactttgagc attccggcga agcctaaaaa ggccattccg
     8641 atgatgccga tcgtgatcag tgtgataccg gcgcctttta aaccagccgg aatcgggttc
     8701 ttctctatcc tttggcgaat ggcggccaag agcacgatcg cgagccacca acccagtccc
     8761 gaacccgccc cgaaaaaggc cgattgcagg aggtcgtaat tgcgaagctg cataaacagg
     8821 gatacgccca taatcgcgca gttcaccgtg atcaagggca agaagatgcc aagaccgcgg
     8881 tagagggtcg gggacacccg ttcaatcacc atttccaaaa cttgtacaat ggcggcgatg
     8941 acgatgatga acacgatgta tcgaaggtaa tccagttcaa agggcacgat aacccagtgg
     9001 tagaccatcc agttgatgag ggtggtgatc gtcaatacca aggtcaccgc catccctaat
     9061 ccgtttgacg ccttcaaatc cttggagata gagatgaacg agcacatacc caaaaagagg
     9121 gagagcagga tgttgttggt gaagacagaa gcgatgagga gcgttaaagg atgcacgtcc
     9181 ggagtcatct tctcacttcc ttttcttcgc gagtaaggtg ttgtgtatcc aaatatagat
     9241 ggcgagaacg aaaaaggcgc tcggagccat aaccataatc gtccagggaa taaactgatc
     9301 cgggagaacg ggtaccccga aaagcgtgcc gaaccccagc agctcacgga agaaggccac
     9361 gatcatcagt accatcaagt aaccgatccc ggaggtcaac ccatcccaaa aagccgcccc
     9421 cggcgggttc gcttgggcga aggcctcggc gcgccccatg aggatgcagt tggtgatgat
     9481 caaaccgatg tagggtccta aagccttcgc gatatcgggt ataaaagctc gtagaaggat
     9541 atccaacagg atcacgtaga aagacaagac tagaacctgt atgatcatcc tgactttttg
     9601 cggaatccaa gctttcatca acgaaagcgt gaagctggaa aaggccgtgg aaagcgcgac
     9661 accgacgccc ataatgaaag tgttgacgag gttgttggta acggcgagcg ccgaacagat
     9721 tccgatgatt tgtgaagcga cgggattgtt gcggaataga ttgtccttca aaatgcgtgt
     9781 ggtgttagtc gctgcgttta ggctcatagg cctccctcct tcactcgttc ctccaagtct
     9841 ttgagggcct ttttcaagat cgtctcgagg gatttcgatg tggacgtcgc ccccgttatc
     9901 gcgtcaacgc tcccgttatc tttatcacgg tcaccgggat cggcctgccc cgtcctgaag
     9961 gcgacggtcc cgttgacgag tttttctcct tggaactggc ctttgaacca aggttcctcg
    10021 atccgccctc ccaaccccgg cgtctcattc tgttccacga aggctatccc ggtgagccgt
    10081 tcgaattggg agtccactcc gatgattcct tggataatgc tccaaaggcc gtttccggaa
    10141 aagaggatcg cgtatttttt atcttcagga ggattggtga aaaagatccg tttcccacgg
    10201 acctctttca cgccgatccg gccccgatag atggtcaacg cctcttggtc gttctgataa
    10261 ggaatttgca tcgattcgag aacggctcgt atcaaaaaga gctcttggtt ttttttgacc
    10321 tgttctttcg tcaactcgtt gacgaaagac aggacgagta cgaatataaa ggtgaccaag
    10381 acggcgaacc cgaccagata ggccttgtga tctttgtatt ctaaaaggtt tagtttcatg
    10441 agcgaaccac cgcctttttc ttcggcatca acaggtcgat cagcggggca aaggcgttgc
    10501 caatcagaac ggcgaaactc attccttccg gaaagaagga aaaggttcgg atgagaacgg
    10561 cggtagtccc aatgatcgta ccgtatatcc ataaggagga agtttttttg gcgccgaaac
    10621 cgggtcggtc gccataaaga ccgtaacgaa cagaaagctg cccgaaaaaa ttgcgggtaa
    10681 cgcgtcaagg gcgcccggca ccttgagcca gtcaaataaa aaggtgaaca gcactccgct
    10741 gagcgcggtc gacaacataa tccgccaaga ggcggttttg gaccagatca ggtagatcgc
    10801 agccgcgatg actagaaaaa tcgggccttc gccgagagaa ccggctcgaa tcccgaaaaa
    10861 cagctcggcg agtccaggcg tctcaccgac cttcaagagg gccaacggag tggccgcggt
    10921 gacggggttc gatccaaaat ggctccacgc gatccatccg gtcgtgagga gattgggaaa
    10981 ggtcaggtag acgaacagcc gcgagacgat cgccggattg aagacgttcc ttccgaatcc
    11041 cccatagact tctttcgcga aaaacacccc gaagactata cccaaaacag cgacccacca
    11101 gggcgtcaca gggggtaagg atagcgtgaa aagcaggcag gttaccaata ccgcttcgct
    11161 gaccttcttt ttacgcccac gctctacgat ccattcggtc aagataccgg cgagaaagac
    11221 ccaagccccc agccataaga cgcgatatcc gtaggcgtaa atcgaaaaga tcgcgatggg
    11281 cagcagagag aagagcacct ttctcatcat tttttgtgtt tgaaaccagg aaaccaccac
    11341 tgtcaccccc gtattttccg atcgaatgtc taattttaac acaaatcctc ggcaagaacg
    11401 ataactcggg tcgactcgtt tggagggtca gtttttgagt ctaaaaatat gccggactga
    11461 agttcggcgt tctcagaaag gcaaaaacag atatatgccg agctgaagtt aagcgttccc
    11521 agtagttcgg ccctagaata aaaccccgat tagtcctctg ccgttactaa atccggactt
    11581 ctgtcgcccg ccctttctct ttgtggtaaa atcgaagcaa ttgagaaagg agaacgaatg
    11641 gagcgcttac gaacggttaa cttgcctgtg gagatgcgca gggtctttgg gtttctgtat
    11701 aaaaattata ccgaggaaga attgaggaac gtgctcgaag cttacgagtt cgcgaagcgt
    11761 caacacgccg aacagtaccg caagagcggc gagccgtaca tcaaccatcc cttgaaagtg
    11821 gctcaaattc tcgccgagat gaaactcgac accgtcagca tcaaagccgg cttgttgcac
    11881 gatgtcgttg aagacacggg gacttctttg caggagatcg aacagcgttt tgggaaagag
    11941 gtggcgcgta tcgtcgatgg cgtaaccaag atcgatcggt tgaagatcga tcagaagaat
    12001 ttcgaccaga aggctgaaac cgtactgaag atgtttttcg cgatgtccca agacattcgc
    12061 gtgattttgg tcaagctggc cgatcggctt cacaacatgc gaaccctcga gtttcaatct
    12121 ccggaaacgc agaaacagaa ggcgaaagag actctgcaga tattcacgcc gatcgcgcac
    12181 cgcttgggta tctacacgat caagtggcaa ttggaagatc tagccttcaa atacctctac
    12241 ccggaagagt acacgcatat tacgtcgctt gtggattcaa aacgcgcgga tcgggagctg
    12301 atcacgcaag agtacttgag cgtgcttcaa ttgaccttgg cgcgaaatgg catccaaagc
    12361 gagatatctg gcagagaaaa gcatttttac tccatatgga agaagatgaa cgagaggggg
    12421 aaaaacttcg acgagatcta cgatctcatc gccattcgtg tcatcaccaa caccgataag
    12481 gagtgttact acgtgttggg gttggtccac aacctgtgga aaccgattcc cggacgggtg
    12541 aaggactaca tcgccacgcc gaaatcgaac gggtatcgct ctttgcacac gacggtcatt
    12601 acgcatcgag gagaaccgct cgaaatacaa atcagggaca aagagatgca caacgaagcg
    12661 gagtttggtt tagccgccca ttggatatac aaagaaggga agatgcagaa gcaccagcag
    12721 acttggctga agaagctaca ggagtggcac caagactatg agcagggtgt taccggcctg
    12781 aacgaattcc aaaaagagat acagctcgaa gaggtctata ttttcactcc caaaggcgag
    12841 ctgaagcata tgcagaaggg cgcgaccacg atcgacttcg cctattcggt ccacaccgag
    12901 gtgggccatc actatgcggg cgcgaaggtg aacggaaaga ttgtcccaat aggttacgaa
    12961 cttcagaacg gtgatgtcgt cgaggtcatc gtcaataaaa acaacccagg ccctagcttg
    13021 gattggctaa aatatgcaaa atcgtcgagc acccgcgcga agatccggcg ctttttcaag
    13081 gaaaaggatg cccaggtgtt gatcgaagaa ggcaagaacc ttcttcggca ggtcgcaaaa
    13141 aaactgcaga gatcgatgga ggacctcctc gaatccgatg agctcaagca atacctcggt
    13201 atcgataggc aagtgagcga cagggatatc ctgctgagaa tcggagaaaa ggagcataac
    13261 gcggaggata tcgtagtttt attccaacct cagccgatag aaccccctcc agttatggga
    13321 ccaccagaca agaaacaggt gcagtctacc actatcgtag ttgacggtca gctcgggatc
    13381 gaggtgaaga tggcgaaatg ctgccttccc gttcccggag acccgattgt tggcgtcata
    13441 agcaagaaag ggatcacgat tcatcagaaa gactgcccta acgccgaaaa cattatgaag
    13501 ggagaattgg tatccgtaca atggggcaac acggatcagg agcggtttat gacccacgtt
    13561 attttggaga ccaatgacca ttcgggcaat ctcttgaaaa acatctgcca atcggctaaa
    13621 gagaggaact accatatcac ggaggttaac acgaaattgg acgtgatcaa gcatatgacc
    13681 taccgccttt cgatcttcgt caaatccttg acgcagctcg aggagatgct ggaatattgg
    13741 agaaaacaaa acggtattgt caacgtttat cggaccaggg gagagggatg aaaacagtct
    13801 tgcagcgcgt aaaacgcgca tctgtgacag tggatggcct attgatctcc gagataggcc
    13861 cgggtttgtt ggtctttctc ggcgtcggag agtccgacga aatcgccgat ctggaatgga
    13921 tggcggaaaa gatcgtgaat ttgagaatct tcgaagacga aaacgaaaag atgaaccttt
    13981 cggtgaaaga tacgggcggc tccatcctcc tcgtctccca gttcaccctg tatggagacg
    14041 ctcagaaggg gagaaggccc tccttttccg aagccgcgaa acccgaaaag gcgaaggtgt
    14101 tttacgaggc ctgtgggaga aggatcgccg aactcggggt cccggtcagt atgggcgttt
    14161 tcggcgcgat gatggcgatc gacctcttga acgatggccc cgttaccatc ttgatcgatt
    14221 ctaagcgata gagctacgcc gtgaaaccgc aacgattact cctgcatatc tgttgcgcgc
    14281 ccgacgcgac ggtcgcgttc gtcaagctca aagaacgatt cgaggtcgaa gggcttttct
    14341 acaatccgaa catctttccc gaggaagagt atcgtaagag gtgttccgcg gcccgggatc
    14401 tcgcagaggc gtggagcttc ttgctccacg agggcccata cgattgggaa agctggaagg
    14461 cgttgactcg cggttacgaa aacgtagacg aaggtgggga gcggtgccaa ctgtgcatcg
    14521 ctcataattt aaggcaaaca gcggttttcg cgaaagcgct tgggttcgga tggttctcga
    14581 cgtctctgtt caccagccga aagaaggacc tgagaatgat agagtctttg ggatatcgga
    14641 tcggcaaaga gacatcggtc caattttact atgaaccctt ccgaagggcg ggggggttcg
    14701 acgagagcgt acggcactcc cgcgagttgg gtctttaccg tcaaacctac tgcggttgtc
    14761 cctattccgc gcaaggcgcg aattccaatg ggccatcggg ttctacccga gcgggatgat
    14821 gaaaacggaa cgacaagaga gctggaagat acaggtcccc tttcatctcg attattgcct
    14881 gaactgcggg caggtgttcc ggtggaaaaa actcgggaat cattggtttg ggtttatcga
    14941 aggccatctt tacaggatcg aatacgagga gtcgagccaa accatccggg cgttacggat
    15001 ttcgggtgac acagggcgaa ccggcgcgga ctcggaaggg gaaagccgtc gccgaataga
    15061 gcgctatttc ggtctctatg actctttgag cgcgattcga gagtctattc tgacgaacct
    15121 gcgagaaaaa gggtataatt ggttcgtagg aaaattcgat gatatatttt cgaaaagcct
    15181 cggtttacgt attctccggc aagacgtgtg ggaggttgcg gtgagctttt tgctgtcctc
    15241 gcaaacgaat atcccagcct tagaacggaa gttggacgcg ctctgtcggt tgtttcctca
    15301 aaatgaggtc cgctatgaag gagagtccta ttacaggttt ccgaagagag agtcgttagc
    15361 gaccttatgc gaagaagatt tcaagcgtct cggatttggg tatcgcagcg cgtggttgtg
    15421 cgaaatggtt agaagatttc gagagtcgga tttccgttcg attcgaacgg agacgtacga
    15481 tcggcagctc tcgtttttgc tttcctttcc cggagtcggc tacaaggtgg cccactgtat
    15541 cctcttgttc gggtattcag tgatgagagc gtttccggtg gacatctgga tctctcgatg
    15601 gattaaagag gtgtttcacg tcgagggcgc tccggatcgg ttgacgtcgt ggggacaaaa
    15661 cgtgtttgga gaatactcag gatatgccca ggaatatatt tttaacgttt accgcaattt
    15721 ggggtctgat gggggagaga tttgacggag cgtctatcta tcgagtttaa tgcgatcatc
    15781 gaccatgtcc gtgtgatccg aggagcgctg aggtcttttc tcagatcgaa ggaaatcaac
    15841 gccacgcaag tcttcgatac cgaactcgcc ctcagcgaag ccgttgtcaa cgtcataaaa
    15901 cacacttatc ggtttgaacc cacgcaaacc gtctatttga cgatcctatg ggagccggaa
    15961 gaacgagcgt gcaccttcct gctggaggat tcgggaaaga ctgtttctcc tgagaagatg
    16021 ctctcgcgtg acctggacga cctgaaggat catggactgg gcgtctacat cataaaaagc
    16081 ctgatggatg aggttaccta ttctccgcgt gaagacggta gcggcaaccg gttgacgatg
    16141 aaaaaacgat tccgaccgta ggcgatgatg atcgaagcgg ttaagatcat cggaggaggg
    16201 ttggccggat gcgaag
//
LOCUS       DAOPXN010000036        16113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3697_length_16113_cov_4.963134, whole genome shotgun sequence.
ACCESSION   DAOPXN010000036 DAOPXN010000000
VERSION     DAOPXN010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16113
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3697_length_16113_cov_4.963134"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PKM49_03335"
     CDS             <1..1864
                     /locus_tag="PKM49_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNY81882.1"
                     /translation="GEPGVGKTAIVEGLTQKIVNQQVPENLKNRIIFSLDIASLIAGT
                     KYRGEFEKRLKKLLLFLVQEPNIIVFIDEIHTIVGAGAAEGAVDAANILKPGLSRGDF
                     RCIGATTPDEYRKYIETDGGLERRFQKLYIQEPTMDETLEILKGLKSRYEKHHNVRYT
                     DEALQESVRLSRTYVSDHFLPDMAIDVIDEAGASKRLKTLMIPDALKEKEERIEEVKR
                     QRNQATLEKNFLFMKKLMMDERELVLEYNRELQLWKANTKSVIEVVDHEDVAKIISNW
                     TGIPLTKLEEDEVTKLLNVEDTLHKRVVGQNEAIEAISKAIRRTRSGLKDPKRPSGSF
                     LFLGPTGVGKTELAKTLSEALFGNEKNLVRFDMSEYMERFALSRLIGAPPGYIGYDEG
                     GSLTEIIRRRPYSVVLFDEIEKAHPDVYNILLQILDEGRLTDSRGRNVDFRNTILIMT
                     SNIGGEIINRSKATVGFGVFESEEEAYQEMKRTVLNEVKKVFRPEFLNRLDEIIVFHQ
                     LTKDHIFQVVDIMISQLEERLAAKNITFELKQNVREFLVEKGFDKQLGARPLKRAIQK
                     YIEDPLSEELLLKRIEPGSVVILYMNAKGEISFKKKKDKENHKPELVSSSSLLP"
     gene            1877..3271
                     /gene="radA"
                     /locus_tag="PKM49_03340"
     CDS             1877..3271
                     /gene="radA"
                     /locus_tag="PKM49_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNY81883.1"
                     /translation="MKEKTVYVCDICGYDNPRWFGQCPQCRNWNTAQEVRQTKRPRAS
                     TEIEGTDATFEALIKKQKEPVTRITTRFNAFNRVLGGGIVPGSVLLIGGDPGVGKSTL
                     LTQLADQKVTVFYWSGEESTEQIMLRAQRTNDATLDRLYVYNTNRLELALEKLQKALS
                     DSESKLPDSFQPVLVVDSIQTMKSDEVSALPGGVVQIRECTAALVAFAKARKVPVIIV
                     AHITKYGQIAGPKLIEHLVDVVLYFDGDSDRDLRILRSIKNRFGPTNEIGVFEMGENG
                     LKEISDPSERFYDANANDSGNAISVILEGSTPFVVEVQALAVSTHFSSPRRLSKGVDQ
                     ERLNLIAAVLSKRLNLPIENHDLYLNLLGGLKCSDPGIEFPIAVAIFSSFFDKTVPPK
                     TAFFGEVGLDGKIRGVSFSEKRITELARLGFSRIYLPKNKAHAGVFKKHPQVDFFECA
                     YLKEALIEIFDGRS"
     gene            3272..3676
                     /locus_tag="PKM49_03345"
     CDS             3272..3676
                     /locus_tag="PKM49_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HNY81884.1"
                     /translation="MVTTRWNGNMSFEAKTPDGKVVLMDVPGTEGALGASPKVLLIVG
                     LTGCTGMDTVSILEKMKVPKYEFRIEADYHSTEEHPKIYDRIHLRYYFHFEGEPPKDK
                     VSKAVELSQTKYCGVSAMLKKAAEITYEILYE"
     gene            complement(3720..4682)
                     /locus_tag="PKM49_03350"
     CDS             complement(3720..4682)
                     /locus_tag="PKM49_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81885.1"
                     /translation="MNAYGSAGSSSALKIWGALLCFLMAFSLLQAAPPTHYSLHIPLS
                     LQRTIQDLIERYGEELDALWGLTLSFPIHVDIQRESGLSVRGEATFDGRLYTVFMTPS
                     FLDLPFLLKHELMHLYTFEWIWRTQRLDIEGTEQRVIYASVPLWVMEGLAVWYEGRLM
                     QDPRIAFPWEYRALINFTKIEEYPKGQAHAQYYIVLSDFFREYDRRANLRERFGDVLR
                     YVEAGMSWPEAFTKATGRDFEEALTRWKRGKTLAVWLSVFLDFLAAGIPLLLLFVLFV
                     HFFRRRNPVDTFGPEYEKKYGPSYWLTSSESEEPPNTAETKNGT"
     gene            complement(4687..5661)
                     /locus_tag="PKM49_03355"
     CDS             complement(4687..5661)
                     /locus_tag="PKM49_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81886.1"
                     /translation="MKRKVILCGLIVVLLCHSLTGAQTLYIDLRKGIALFERTPNLWE
                     VLPVLSTNLRGAPRFEVGITQVYRPPDTRYVLPFLPPVALRLENGLVITNREGYGDIA
                     LELPTEKTVFERIKRIASISLPWTFEEKALVIEDAEWLLFLPPYLNYLGKGNLIDQAM
                     SRAKLGVFPLKIPLEDEPLQIEVQVGLSYPGWPVFARLQANQPLTRCLWLEGVNELRS
                     LLDFRKDRPGLYTLTLFVEDIYGRTMTKEVPITISEFKVPVATQEITDKTTVGSRLLF
                     TQRVSGVWNLWDTQIVGTRASLAFPFPGTFDILFTAPKIRIRYHIVVE"
     gene            complement(5658..6731)
                     /gene="ispG"
                     /locus_tag="PKM49_03360"
                     /gene_synonym="gcpE"
                     /pseudo
     CDS             complement(5658..6731)
                     /gene="ispG"
                     /locus_tag="PKM49_03360"
                     /gene_synonym="gcpE"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
     gene            complement(6867..8591)
                     /gene="thrS"
                     /locus_tag="PKM49_03365"
                     /pseudo
     CDS             complement(6867..8591)
                     /gene="thrS"
                     /locus_tag="PKM49_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487327.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            8951..9622
                     /locus_tag="PKM49_03370"
     CDS             8951..9622
                     /locus_tag="PKM49_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81887.1"
                     /translation="MAEKAKPAKKKNGKLIFLTLTVILTVGLLVLIFGYIDFEFKKIG
                     EAGFSPLSDWRSYLAFLMSKVPIVKNYVKYEPRKIVKPSVFYTEIYESYIEKIETDRQ
                     ELENRAEELDKKDKRLKQLEAEINTRTQELDLKEERLLTETNNWEDQQKRYRQLAEWY
                     ENGETAKIALALSSDRISIEEIVSGLRFVEPGIASEIVGALANLNPDKAAQILAYLAG
                     KEVIR"
     gene            9619..12075
                     /locus_tag="PKM49_03375"
     CDS             9619..12075
                     /locus_tag="PKM49_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNY81888.1"
                     /translation="MNRIDQSLTDQLFSTKASSRLTDAENSVKSEYATSLFSQKESGS
                     RENTFTSAFNQERRAVRSSASEEPKEDRQTNSAEKEKTGAVVTRKKTRKPNSSKTSSG
                     VELDGESDWAPLSEEDSEPDQAKKAREGGLDPMWLALVNSEALTMVEPLAEGGAFQLE
                     RLLWNESGEKARAQALGLSVWIQLSEGKSVSVTTVDTQPLSTELIQAAPQIAEGGEGE
                     RASTLGDQLALDTLDPLAPSVLGKVETADPSVIANVGDPHEKPLKIEEPKTTPSNPQP
                     DPGPSLEGSLHGKTVASDTADTLQSVWNGGKDPLPETTELADSPQNLKNTPDRRVVDG
                     SKLGDAELALTKGAYVIREAGLDDPKPEGSTRPLESEATVNTGSKITAALAETLREAQ
                     NATNTTLPVQEEVAGSSVTKAFNPTERSTVAVQTQKPVNNPPLRAAKDINTNEKGPSL
                     LPEKMGYPANANSKEEEGQRQPGVSESSGALRKTAKYVPNPNLSNPAVKQSNTPTLWD
                     RYQEIFFEKDIQKVRLDSFTVKVFQKPPSPTEVSFDSAKPLERREGQQVTSGLKSVEN
                     PVFRMPTPIKASAYQDIGAVRGQDIQATLSGIEEMKASRSLAWEGSETSQLKVPQQTD
                     DSTKTLGLAQVPAKYNGSKEDQGDSQAMHQQSDGTWRAQGWGSKEVSLTKDWEANLRE
                     IAQRIKDLASISSSRMRTVESAVIRLNPPELGRMVVEVVKEGNSVVVLLKVETQEAKE
                     MLEKNAHLLTQRLAQSGFEAQKVQVMMEKYEEQGQGRQDAQQERGSEQDQSRKEQETP
                     KENDTEEPVYQSFAELLAGV"
     gene            12099..12800
                     /locus_tag="PKM49_03380"
     CDS             12099..12800
                     /locus_tag="PKM49_03380"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNY81889.1"
                     /translation="MWMDSINNTKGISNTGDPTKKASGLGAMGKDVFLRLLITELQNQ
                     NPLEPMSNQDFVNQMVQFQNMEQMSNMSQSLQDYLGTLNATTKMQAASIVGKYVVIEG
                     NEVSLSKGLADPILYSMEKDGKVMIKIKNEDGQVVRTENLGYKEIGLQNYRWDGKNDY
                     GAVLPDGTYTYQLSTIDENGKQTEFGGVIGGIVQAVQYADNEIYVILNGEKYNYKKII
                     EVSATIPQEGTTEEA"
     gene            12883..13314
                     /locus_tag="PKM49_03385"
     CDS             12883..13314
                     /locus_tag="PKM49_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HNY81890.1"
                     /translation="MAEDLKNQLNINNQRSTPLYNTRLGKSQLKHGEVTEGSFADVLQ
                     SVRQEEAPLGVKISSHAQKRIQSREIKVDEATLKTLDQVLALGAEKGSRSTLVMAKNQ
                     AFILSIKDRTLVTALKREEMDRYYFTNIDSVFVSPEEKETL"
     gene            13395..15968
                     /locus_tag="PKM49_03390"
     CDS             13395..15968
                     /locus_tag="PKM49_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNY81891.1"
                     /translation="MMRSLFSGISGLKGFQGSMDVIGNNISNVNTVGFKASRTTFQSM
                     LLQTMIGAKAPDEGGRGGVNSLQIGLGAQIASVDKMMTQGSFQNTGSKTDLSISGDGF
                     FVLSDGIQKFYSRAGNLSLDEKGNFVQPTTGLKLQGWIASIDAKTGKRYIDTDKPVAD
                     LFVTAGMTMAAKATVNMDIAGNLSAQTGIEPVTITVQNPNDTTKTYKVRFTFEKVPPF
                     NTDRLFSGTSITYTWKAEIVDWNASSPNEIPELDSTIGRIELDQFGQIVKFEKYATNA
                     LFGMIDTSTGGLTFDSSIIDGDSVRTWFNYSPSGSAAITLAVRDLFDPDQDKVPNKIL
                     TGASLKLKASLTATVTTGEYLFTPPPTSAGQTTSIRLHESVSPATGDKLTVSYFTQED
                     LAATSSKTQYRITGPIVDIVGANDITEVDMVVRLSDGAGNFSTAVLTAGTQYTYDANT
                     GTVTIIDTSALSGSSKIRIEYKTQESFIGDGTRTEFQLSKRMINKPPSLTAILTADAY
                     LDLNFVLKEEYGRMEFRKNYPTTGFFENYTIKYADPSNPTDFDVVNVKVSNATKASVS
                     IPKTGKIKFFDKNDTQNYALGSYIAPKYTTTVQVYDSLGKAYNLSIEFQKISTNKWAW
                     RAIEESGMVVSFVDDKERVYTPNPVDATSIVGGIVEFDSGGTVSAFKAIRQNGQIVED
                     VSITRIKFDPGVFVDDGAAPPPEEGATAVTVQIDFSELVQFAGAFSAAVSEQDGNAMG
                     VMQNFAINVSGQVIGTFSNGKSEALGQIALAIFNNPGGLQDLGNTLFTVTPNSGVPMI
                     GIADVGGRGTISPGVLEMSNTDLSEEFTGMVIVQRAFQANARTITTSDEILQELVNLK
                     R"
BASE COUNT         4207 a         4147 c         4322 g         3437 t
ORIGIN      
        1 tggtgagccg ggagttggga aaaccgctat cgtggaaggc ttgacccaaa agatcgtcaa
       61 tcagcaggtg cccgagaatc tgaagaacag gatcatcttt tccttggata tcgcttcctt
      121 gatcgcggga acgaagtacc gaggagagtt tgaaaaacgg ttgaaaaagc ttctgctgtt
      181 cttggttcag gaaccgaaca tcatcgtctt catcgacgag atacacacca tcgtcggtgc
      241 cggcgcggca gaaggggcgg tggacgcggc gaacatcttg aaacccggcc tatctcgggg
      301 cgattttcgt tgcataggcg cgacgacgcc cgatgagtac cggaagtata tcgagaccga
      361 cggtggcttg gaaaggcggt tccaaaagct atatatccaa gagccgacga tggatgaaac
      421 cctcgaaatc ctcaaagggt tgaagtctcg gtatgaaaag catcataacg tacggtacac
      481 ggacgaggct ctacaagagt ccgttcgttt gtctcgaacc tatgtctccg accatttttt
      541 gcccgatatg gcgatcgatg tcatcgatga agctggcgcc agcaaacgtc tgaaaacctt
      601 gatgattccg gatgcgctta aagaaaaaga ggaaagaata gaagaggtga agcggcaaag
      661 aaaccaggcg accctcgaaa agaatttcct gtttatgaaa aaacttatga tggacgaacg
      721 agagttggtt ttggagtaca accgcgaact ccagctatgg aaggccaaca cgaaatcggt
      781 catcgaagtg gtggaccacg aagatgtggc gaagatcatt tccaattgga ccggcatccc
      841 gttgacgaag ttggaagagg acgaggtcac gaaactgcta aacgtggagg atacccttca
      901 taaacgggta gtcgggcaaa atgaagcgat cgaagccata tcgaaggcga tacgccgtac
      961 ccgcagcggc ctcaaagacc caaaaagacc aagtggttct ttcctattct tggggcctac
     1021 gggggtgggc aagaccgagc tggccaagac cctgtccgaa gcccttttcg gcaacgaaaa
     1081 gaacctcgtg agattcgata tgtccgagta tatggaacga ttcgctctct cacgccttat
     1141 cggggcccct cctggctata tcggttatga cgagggcggc tccttgacag agatcatacg
     1201 ccgcagaccc tattcggtcg tgctcttcga cgagatagag aaagcccatc cggacgtgta
     1261 caatatcttg ttgcagatct tggacgaagg ccgattaacg gactcgagag gtagaaacgt
     1321 cgatttcaga aacaccattc tgattatgac gagcaacatc ggcggggaga tcatcaaccg
     1381 ctctaaagcc acggtcggat ttggcgtctt cgaaagcgag gaagaggcct accaagagat
     1441 gaaaagaacc gtcctcaacg aagtaaagaa ggtttttcgg ccggagttct tgaaccgttt
     1501 ggacgagatc atcgtcttcc atcagctcac gaaagaccat atcttccaag tcgtcgatat
     1561 catgatctcc caattggagg agaggctggc agcgaaaaac atcaccttcg aactgaagca
     1621 gaatgtgcga gagttcttag tggaaaaagg gttcgacaag caattgggcg ccaggccttt
     1681 aaagagggcg atacagaagt atatcgaaga ccctttgtcc gaagaactcc ttctgaaacg
     1741 catcgagccg ggaagcgtcg ttatccttta tatgaacgcg aaaggagaga tctcttttaa
     1801 gaaaaagaag gataaagaaa atcataaacc cgaattggtc tcctcttcct cgcttcttcc
     1861 ataagggtaa gccctcttga aagagaagac cgtatacgta tgcgatattt gcggctacga
     1921 caacccgaga tggtttgggc aatgtccgca atgtcggaat tggaatacgg cgcaagaggt
     1981 gcggcagacg aaacgtccga gagcatccac ggaaatcgag gggacggacg cgaccttcga
     2041 agcgttgatc aagaaacaga aagaaccggt aacccgaatc accacccgtt tcaatgcttt
     2101 caatcgcgtt ttgggtggcg gaatcgtgcc tgggagcgtc cttctcatcg gaggagaccc
     2161 cggtgtggga aagtctaccc tcttgacgca gctcgcagat cagaaagtca cggtcttcta
     2221 ttggagcggc gaagaatcga cagaacagat catgctcagg gcgcaaagaa cgaatgacgc
     2281 caccctcgac cggctctacg tctacaacac caaccgattg gaattggcgc tggaaaagtt
     2341 acaaaaagcg ttatccgact cagagtcaaa actccctgat tcctttcaac cggttttggt
     2401 cgtggactcg attcagacga tgaagtcaga tgaagtgagc gcgttaccgg gcggggtcgt
     2461 gcagattcga gaatgcaccg cggcgttagt cgccttcgcc aaggcgcgca aagtgccggt
     2521 gatcatcgtc gcgcatatca cgaagtacgg gcaaatagcg gggcctaagc tcatcgaaca
     2581 cctcgtggac gttgttctct atttcgacgg ggactcagac cgggatctac gtattttacg
     2641 gtcgatcaag aatcggttcg gccccaccaa tgagatcggc gtctttgaga tgggagagaa
     2701 cgggctcaaa gagatttcgg acccttcgga gcgtttctac gatgcgaacg ctaacgattc
     2761 gggaaacgcg atttccgtga ttttggaggg cagcacccca ttcgtcgtgg aggttcaagc
     2821 cctcgccgtt tcgacccatt tcagctctcc gcgtcggttg agcaaagggg tcgatcagga
     2881 acgcttaaat ctaattgccg cggttttaag caaacgtctg aacttgccta tcgagaatca
     2941 cgacctgtat ctcaatcttt tgggggggtt gaagtgcagc gaccccggta tcgagtttcc
     3001 gatcgccgtg gcgattttct cctctttttt tgacaaaacg gttcctccga agaccgcctt
     3061 tttcggtgag gtgggattag acgggaaaat acgcggcgtc tccttttccg aaaagcggat
     3121 taccgaactg gcgagattgg ggttttcacg aatatatcta ccgaaaaaca aggcccacgc
     3181 gggggtcttc aaaaaacatc cccaagtgga ttttttcgaa tgcgcctatt taaaggaggc
     3241 gctcatagaa atcttcgacg ggaggtccta gatggttacg acacgttgga acggaaatat
     3301 gagttttgaa gccaaaacgc ccgatggtaa ggttgtttta atggatgtgc cggggactga
     3361 aggggccctc ggggctagcc cgaaggtcct gctcatcgtg gggttaaccg gttgtaccgg
     3421 aatggacacg gtttctattt tagaaaaaat gaaagttcca aaatacgagt ttcgtatcga
     3481 agccgattac cactcgacgg aagaacaccc gaagatctac gaccgcattc acctgaggta
     3541 ctacttccac tttgagggag aacccccgaa agataaagtg tccaaagcgg tcgagctttc
     3601 gcaaacgaag tactgcggcg tctctgcgat gcttaagaaa gcggcggaga tcacctacga
     3661 gattttatac gaatgagttt gtcgatttgc gaagagccac tttttaacgc gtctggccgt
     3721 taggtaccgt ttttcgtttc cgcggtattg ggaggttcct cgctttcaga agaagtcagc
     3781 cagtaggacg gaccatactt cttttcgtat tcgggaccga aggtatccac cgggtttctt
     3841 ctacggaaaa agtggacgaa caatacgaaa aggaggagga gtggaatccc agccgccagg
     3901 aaatctagaa agaccgaaag ccatacggcc agtgtctttc ccctcttcca gcgggtcagg
     3961 gcttcttcga aatcgcgccc agtcgctttc gtgaaagcct ctggccaaga catacctgct
     4021 tccacataac gtaacacatc gccaaagcgc tctctcaaat tcgcgcgccg atcgtattcc
     4081 cgaaagaaat cggataaaac gatgtaatac tgggcgtgcg cctgcccttt cggatactct
     4141 tcgatctttg taaaatttat gagcgcgcga tactcccaag gaaaagcgat gcgcgggtct
     4201 tgcatcaacc gaccttcgta ccagaccgcg agcccttcca taacccataa gggaacggac
     4261 gcgtaaatca cccgttgctc cgtaccttcg atgtccaatc gttgagtccg ccaaatccat
     4321 tcgaaggtgt acagatgcat taactcgtgt ttcagtagaa acgggagatc cagaaaactg
     4381 ggggtcataa aaaccgtgta aagacgacca tcgaaggttg cctctccccg aacgctcagc
     4441 ccgctttccc tttgtatgtc cacgtggata gggaaagaca aggtcagtcc ccagagggcg
     4501 tccagttctt ccccataccg ctcgatcagg tcttgaatcg tcctctgcag ggacaagggg
     4561 atatggaggg aataatgcgt gggaggggcg gcttgaagaa gcgaaaacgc cataagaaag
     4621 cagagcaacg cgccccagat cttgagggcg gaagagctgc ccgcactacc gtaagcgttc
     4681 acgggttcac tccaccacga tgtggtaacg tatgcggatt ttcggcgcgg taaacaggat
     4741 gtcaaaggtg ccggggaagg gaaaagcgag cgaggcgcgc gtgcctacga tttgggtatc
     4801 ccagaggttc caaacgcccg aaaccctttg cgtgaacaac agccgagagc caacggtcgt
     4861 cttgtccgtg atctcctgcg tggctacggg gactttgaat tcgctgatcg tgatcgggac
     4921 ctctttcgtc atcgttcgac catagatgtc ttcgacgaac aacgtgaggg tgtatagtcc
     4981 ggggcggtct ttacggaaat cgaggagaga gcggagttcg ttgacccctt ccaaccatag
     5041 gcaacgggtt aaaggctgat tcgcttgcaa acgagcgaat accggccacc cgggatagga
     5101 gagaccaacc tgtacctcta tttgtagcgg ctcgtcctcc aacgggattt tcagcgggaa
     5161 aacgcccaat tttgctcgtg acatcgcctg gtcgatgagg ttgcccttac ccaagtagtt
     5221 taaataagga ggaaggaata gaagccattc ggcgtcctcg atcaccaaag ctttctcttc
     5281 gaacgtccac ggtagagaga tcgatgcgat ccgttttatg cgttcaaaaa cggtcttttc
     5341 ggtgggaagc tctagagcga tgtccccgta cccttctctg ttcgtaatga ccaagccgtt
     5401 ttcgagacgt aaggctaccg gtggcaggaa gggaagaaca taacgggtgt ccggaggccg
     5461 atagacctgc gtgatcccta cctcgaaccg cggagcgccg cgcagattcg tggaaagaac
     5521 cggcagaacc tcccataggt ttggggttct ctcgaacagc gcgatcccct tacgcagatc
     5581 gatgtagagc gtctgcgccc cggtcaaaga atgacagagg agaacgacga tcagcccgca
     5641 aaggatgacc tttcgtttca tcgaggggga ttgcctcctc cttcttcctg tatccagcgt
     5701 cttacccgcg cttcgaaggc cggaaacagt tcgcttttcg ggtagcgtcc caggatctcg
     5761 cctttttcga acagcgcccc accccccggt atgccggcga tccctaagtc cgcatttttc
     5821 ccttctccga ttccattgac cacgcacccc ataatcgcta ttcgcacagg cttaacgcac
     5881 gctctcgtca actcggtgac cttcaatgcg tattcttcca catcgataca cgttcgcgcg
     5941 caggtggggc acgaaatgat atccgggccc gacaacaagc ctgcggcctt caacagggtc
     6001 ttcgccgcgt ccacttcctt gaccgggtct ccgctaatcg agtatcgaat cgtatccccg
     6061 atcccttcgg acagaagcag gccgattccg agcgtgcttt tcaccaggga gatcgacagc
     6121 ggaccggctt cggtcacgcc gagatgcaat gggtagctta cagagctgga gacacggcga
     6181 gtggcttcca ccatctccgt cacgttcgaa gacttgaccg cgatgacgat ccggctcata
     6241 ccggcatcct cgagcagctt cacttcccgt aaagcggaag cgaccaaggc ttcgacgacc
     6301 ggcagatgtt cgaaattttt tgccaaggaa ccagaattgg cgccgacgcg tataggaacc
     6361 cctcgttctt cggctgctat cgcaatctct ttcactttcc agccctcacc gatattgccg
     6421 ggattgattc ggaccttatc aattcccgcc cgaatggccc ccaaggcgat acggtaatcg
     6481 taatggacat cggcgatcag ggggtagtct cccgtctctt ttcgtatctg ccgcaagctg
     6541 tcgacgcact cgaggtcata gagcgcgagc cgaatggcct cgcaccctgc ggcgcgcaaa
     6601 cacgcaatct ggcgcaaggt cgcgcccacg tcttttgtat cggtgttcgt catcgattgt
     6661 atcgcgatcg gatatcctcc tccgatcgtg atcgacccca cccgaatggg tattgtcatc
     6721 ttccggatcg ttttatccct ccgttcacgc cgcctctcgc gcgtgcgctt aaggtggaaa
     6781 cggcgcgtct ataaaggaga cagagacgcg ccgaagcaat tgggatttat tcttgattca
     6841 ggtatttaat gactaagcgt tttcggtcat tcgaacagtg aatgatccgc cagctctttc
     6901 acttctttct cgaagacgtc gatgaaggag tcgagcgtga tcgcgccgag gtctttctcg
     6961 tttcgggttc gcaacgcgat cttttcctcc tccacctctt tgtcgcctac caccatcata
     7021 tagggcactt tattcagctg tgcgtcgcgt atcttatagt tgattttttt ggatcggcta
     7081 tccacttgcg cgcggatgcc tctgttcgca agcgtctctt tcactcgttg gcaataatca
     7141 ttgtgacggt cggcgatcgg caagacggag acttggaccg gcgccagcca ggaagggaat
     7201 gcgcccgcgt aatgttcgat caggataccc ataaaccgtt cgagcgagcc gaagacggtg
     7261 cgatggatca tcaccggtcg gtgttcttgg ttatcttgac cgatgtaggt catatcgaaa
     7321 cgttccggca tctggaaatc gagttgaacg gtcgcgcact gccaagttct cccaaggcag
     7381 tctttgatgt gaaaatcgat ctttggcccg tagaacgcgc cgtcaccttc gttgatgcgg
     7441 tagtccactt gggtctctac aagggctttt ttaagcgact ccgttgccag ctcccacagt
     7501 tcgtccgacc ccatagagtt ctccggcctt gtgctgaggt cgacccggta ctcgaaaccg
     7561 aagaccttgt acatccggtc gatcaaatga atgaggtccg tcgcctctcg aacgatatca
     7621 tcttcggtca taaaaatatg cgcgtcatct tgggtaaagg accggacacg gaaaagcccg
     7681 tggagaacgc cgcttctttc gtaacggtgc accttgccaa attcggccat cttccacggt
     7741 agttctcggt aagaacgcgc ctgactcttg taaatgatga tgtgccctgg gcagttcatc
     7801 ggtttcaccg cataggtttg ctcgtccttc tcgatgaagt acatattctc gcgatagtgg
     7861 tcccagtgcc cggactgtcg ccacaacttt tcgtgcatca ccaaaggggt gatgacctcg
     7921 atgtacccgg cttctttgtg gaattttcgc caaaccgtct gtaattcggt taaggcaatg
     7981 acgccctttt ccaagaagaa gggcattcct gccgctattt ccgtatcgat gtggaacagc
     8041 cccaattgcg gacctaaccg ccgatgatcc cgtcgtttgg actcttccaa gaactcgaga
     8101 tagtccttga gggcctcatt ggtcggaaat gcggttccat aaatacgttg aagcatctgg
     8161 ttcttttcgt ctcccctcca ataagcgccg gcaagcgaaa gcagcttgaa ggggcggatt
     8221 attccagtag aagacagatg tggaccgcgg cataggtcga cgaagtcgcc ctgctcataa
     8281 aaagaaatgg gttcgccttc cggcaggtgt tcgatcaatt ccaccttgta aggctggcct
     8341 ttctctcgca ttaaggcgat ggcttcttct ttcggtaaag tgaagtggcg aatcggataa
     8401 ttctctttga tgatccgctc catctctttt tcgatcttcg gcaggtcctc ttcttgaacc
     8461 gctccattat ctatgagaat gtcgtaatag aaaccgtttt cgatcgttgg gcctatgccc
     8521 aagaccacat tctttacccc atagatgtga gtcaccgctt gggctaagat gtggctcatc
     8581 gtgtgacgat acacgctctg cgcctcagta gtatcgcgat gcacccattc gatctcatat
     8641 tcgccgctgc ccatctcgcc taccgtgcga gtgagatcgg tagtcccgtt tttggttttc
     8701 acagcgaccc attctttcca acggtctttt ttttcggact tcaaaatttc cagaaatgtc
     8761 tgctcgggtg tgacatcgat cttgactctt tctgggttca cgataaggcc catagcgctc
     8821 ataaaacacc tcctcaagac tcctttacta agggagtcga aatttcacat attgtagcac
     8881 actctcccgc ttttaggaaa acccgcggct cttatggaaa aataccgtaa acccagtcgc
     8941 gaggtgctcg ttggcggaaa aagcgaaacc ggcgaagaaa aagaatggca aactcatctt
     9001 tctgaccctg actgtcatcc tgacggttgg gttgctggta ttgattttcg ggtatatcga
     9061 tttcgaattt aaaaaaatcg gagaagctgg tttttccccg ctaagcgatt ggcgtagtta
     9121 tttagcgttt ttaatgtcca aggtcccgat cgtgaaaaac tacgtgaaat atgagcccag
     9181 gaagatcgtt aaaccgtcgg tattctacac cgagatctac gaatcctata tagagaagat
     9241 cgaaacggat cgacaagagt tggagaatcg cgccgaagaa ctggataaaa aagacaaaag
     9301 gttaaagcag ttggaagccg aaatcaacac gcgtacccag gaactagacc tcaaagagga
     9361 acgactgctg accgaaacga ataattggga ggaccaacaa aaacggtaca ggcagttggc
     9421 cgaatggtac gaaaacggag aaacggcgaa aatcgcctta gcgttgagct ccgaccgtat
     9481 ttctatcgaa gagatcgtta gcggattgag gttcgtcgag cccggaatag cctcggaaat
     9541 cgtcggagcc ttagcgaacc taaatccgga taaagcggcg caaatactgg cctatcttgc
     9601 aggaaaggag gtgataagat gaacaggatc gatcaaagcc ttacggatca actcttctcc
     9661 acgaaggcat catcgcggct aacggatgcg gagaactccg taaaatccga atacgccacg
     9721 agcctctttt ctcaaaagga gagcggttcg cgcgagaaca ccttcaccag cgcgttcaat
     9781 caggaaaggc gcgccgtgcg ttcctcggct tccgaagagc cgaaggaaga ccggcagacg
     9841 aactcggcgg aaaaggaaaa gaccggagcg gtcgtcacaa gaaaaaaaac gcgtaagcca
     9901 aactcttcca agacctcatc aggtgtggaa ctcgatggcg aaagcgattg ggctcctcta
     9961 tcagaggaag attcggaacc agatcaagcg aaaaaggcac gagaaggcgg ccttgacccg
    10021 atgtggctcg cgctggtcaa cagcgaggcg cttactatgg tagagccttt ggccgagggc
    10081 ggcgcttttc aactcgagag acttctttgg aacgaatcag gagaaaaagc gcgggctcaa
    10141 gcgctcggtc tttcggtttg gatccaactc agcgagggta aatccgtgag tgtaacgaca
    10201 gtagacacgc agcctctatc gacagagctt atccaagccg caccccaaat cgcggagggg
    10261 ggagaaggag agagggcatc caccttgggt gaccagctcg ccctggatac cttggacccc
    10321 ttggccccct cggtcttagg taaggtggaa actgcggatc cgtcagtcat cgcgaacgtc
    10381 ggagaccctc acgaaaaacc tctaaaaatc gaagagccga agactacccc atcgaatccc
    10441 cagccagacc ctgggccatc gctagagggg tcgcttcacg ggaaaaccgt agcgagcgat
    10501 acggccgata ctctccaatc ggtgtggaac ggcgggaagg acccgcttcc ggagacgaca
    10561 gaacttgccg acagccctca aaatctaaag aacacgccag atcggcgcgt agtggacggg
    10621 tcgaagcttg gagatgccga acttgcttta accaaaggag cgtatgttat ccgcgaagca
    10681 gggctcgacg atccgaaacc cgagggatcg acgagaccgt tagaatcgga agcgaccgtc
    10741 aatacgggat cgaaaattac ggcagcgctt gcagagacac tgcgagaagc gcaaaacgcg
    10801 acgaacacga cgctaccagt tcaagaggaa gtcgccggat ccagcgtaac gaaagcgttc
    10861 aacccaaccg aacgttccac ggtagcggtg caaacccaaa aacccgtgaa taatccacct
    10921 ctccgcgcgg cgaaagacat aaacaccaac gaaaagggac cgagcctttt accggagaag
    10981 atgggctacc ccgccaacgc gaattcgaaa gaggaggaag gccaaagaca gccgggcgtt
    11041 agtgaaagct ccggcgctct gcgaaaaacc gcgaaatacg ttccgaaccc gaatctctcg
    11101 aacccggcgg tgaaacagtc aaatacgccc acactttggg atcgctacca ggagatattt
    11161 ttcgagaaag acattcaaaa ggtccggctc gactcgttca cggtgaaggt ctttcaaaaa
    11221 cccccatcgc caacggaggt ttcgtttgac agcgcgaagc cgttggaacg ccgtgagggc
    11281 caacaggtga cgagcggttt gaaaagcgtc gagaaccccg tctttcggat gcccactccg
    11341 attaaagcga gcgcttatca ggacatcggg gccgtacggg ggcaggatat ccaagccacg
    11401 ctttcgggta tcgaagagat gaaggcgagc aggtcgctcg cgtgggaagg gtctgaaacg
    11461 tcccaattaa aagtgcccca acaaactgac gattccacaa aaaccctcgg cctcgcacaa
    11521 gtccctgcga aatataatgg ctcgaaagaa gatcaagggg attctcaggc gatgcatcaa
    11581 caatccgacg gcacatggag agcccaaggg tggggaagca aggaggtttc cttaacgaaa
    11641 gactgggagg cgaacttgcg tgaaatcgcc caacggatca aggatctggc ctcgatatcg
    11701 tcatcgcgga tgcgaacggt ggaatcggcc gtgatccgcc tgaatccacc cgaattgggg
    11761 cggatggtcg tagaggtcgt aaaagaaggg aattccgtcg tcgtccttct gaaagtggaa
    11821 acgcaagagg caaaggagat gctggaaaag aacgcgcact tgttgactca aaggctggcg
    11881 cagtccgggt ttgaagccca gaaggtgcaa gtgatgatgg aaaagtacga agagcaaggt
    11941 caagggcgac aggatgcgca acaggaaagg ggttccgagc aagaccaatc taggaaggaa
    12001 caagaaaccc caaaagagaa tgatactgag gagcccgttt accaatcctt cgccgaactc
    12061 ttggcgggcg tttagtttag accacggagg tgaaatctat gtggatggat tcgatcaaca
    12121 acaccaaagg catttctaat accggcgacc caacgaaaaa ggcgagcggt ttgggcgcga
    12181 tgggcaaaga cgttttcctt cgcttgttga tcacggagct gcagaatcag aacccgctcg
    12241 aaccgatgag caaccaggat ttcgtcaacc aaatggttca gtttcagaat atggagcaga
    12301 tgtccaatat gagccagtcc ttacaggact atcttgggac cttgaacgcg acgacgaaga
    12361 tgcaagctgc atctatcgtt ggaaagtacg tcgtcatcga aggaaatgag gtgtctttgt
    12421 cgaaagggtt ggctgatccc atcttgtatt cgatggagaa ggacggcaaa gtgatgatca
    12481 agatcaaaaa tgaagatggt caagtggtca gaacggaaaa ccttggatac aaagagatcg
    12541 gcttacagaa ctaccgctgg gatggcaaaa acgattacgg agcggtgttg cccgacggca
    12601 cttacacgta ccagctttcc acgatcgacg agaacggaaa acaaacggag ttcggcggcg
    12661 ttatcggcgg aatcgttcaa gccgtgcaat atgcggataa cgagatctac gtgatcctca
    12721 acggggaaaa gtacaactac aaaaagatca tcgaggtatc cgccacgatt ccccaggaag
    12781 gaacaacaga agaagcgtaa cgagaggagg tgtgaaggaa ggacgggtcc cgtgcatata
    12841 tgctcgagtt aattacgaaa cgggcttagg cttccgatcg aaatggcaga agatttaaag
    12901 aatcaactca acatcaacaa ccaacgttcg acaccccttt acaatacgcg tttaggaaaa
    12961 agccagttaa aacacggcga agtaacggaa ggcagttttg cggatgtgtt gcaaagcgtg
    13021 cgccaagaag aggcccccct gggcgtgaag atctcttcac acgcgcaaaa acggatacag
    13081 agccgagaga tcaaagtgga cgaagcgact ctgaaaaccc tggatcaagt actggccctc
    13141 ggggcggaga aagggtcgcg cagcaccctc gtgatggcga aaaaccaggc cttcatactc
    13201 agcatcaaag accggaccct ggtgaccgcc ctcaagagag aagaaatgga tcgatactat
    13261 ttcaccaaca tcgatagcgt attcgtaagc ccagaagaaa aagaaacact atagaggctg
    13321 gtcctcgata gagggagcct cggagcgggg acggttcgaa ccgctccaac acaaaaatcg
    13381 aggaggtaga tattatgatg cgctccctgt tcagtggaat ctcaggtctt aagggattcc
    13441 agggttcgat ggacgtcatc ggaaacaaca tatcgaacgt caacaccgta ggattcaaag
    13501 cctcccgcac gaccttccaa tcgatgcttc tgcaaacgat gatcggagcg aaggcgcccg
    13561 acgaaggcgg gcgaggcggc gtcaacagtt tacagatcgg attaggcgcg cagatcgcca
    13621 gtgtggacaa gatgatgacc caaggcagct tccagaatac cggaagcaaa acggacttgt
    13681 ccatttcggg cgacggtttt ttcgtgctgt ccgatggcat acagaagttt tactcgaggg
    13741 ccgggaatct atcgttggat gagaagggga atttcgtaca accgacgaca gggctgaaat
    13801 tgcaggggtg gatcgcgtcg atcgacgcga agacgggaaa acggtatatc gacaccgata
    13861 aacccgtggc agacctgttc gtcacagccg gtatgacgat ggcggcgaaa gcgaccgtga
    13921 atatggacat cgcggggaac ttgagcgcgc agaccggtat cgagccggtg acgatcacgg
    13981 tgcaaaaccc aaacgatacc acgaaaacct ataaagtgcg gttcaccttc gaaaaggtcc
    14041 cgcccttcaa caccgaccgg ctgttctcag gcacctcgat tacctacacc tggaaagccg
    14101 aaatcgtcga ttggaacgca tcctctccaa acgaaatccc ggagttagac tccacgatcg
    14161 gtcgcattga gctggatcag ttcggtcaga tcgtaaagtt cgaaaaatac gcgaccaacg
    14221 cactcttcgg aatgattgac acgtcaacgg gagggttgac tttcgattcg agcattattg
    14281 acggcgatag cgtgagaacc tggtttaatt attcaccttc tggttctgca gctattactt
    14341 tagcagtacg agatttgttc gatccggatc aagacaaggt gccgaataaa atcctcacgg
    14401 gtgcatcttt gaagttaaaa gcttcactta ctgccactgt cacgaccggt gagtacctat
    14461 ttactcctcc gccaacaagc gcgggacaga cgacttctat ccgcttacac gagtcggttt
    14521 cgcccgcaac gggcgacaag ttgacggtat cgtacttcac ccaagaagac cttgccgcca
    14581 catcttcgaa aacgcagtac cgtatcactg gcccgatcgt ggatatcgta ggggctaatg
    14641 acattactga agtcgatatg gtcgtccgtc tttctgatgg cgccggcaat ttctccacag
    14701 cagttctgac tgctggaaca caatacacgt atgatgccaa tactgggaca gtgacgatta
    14761 tcgacacgtc agcattgagc ggctcgagca aaattcgcat tgagtacaaa acgcaagaat
    14821 ccttcatagg agacgggacc cggacagaat ttcaactttc aaaacggatg ataaataaac
    14881 cgcccagtct gactgcaata ctcaccgcag acgcctatct cgacttaaat ttcgttctga
    14941 aagaggaata cgggcgcatg gagttccgaa agaattaccc cacaacaggc ttttttgaga
    15001 actacacgat caaatacgcc gatccgagca acccgacgga cttcgatgtc gtgaacgtca
    15061 aagtcagcaa cgcgacgaag gcctccgtgt ccataccgaa gacggggaaa atcaaattct
    15121 ttgataagaa cgacacccag aactatgcgt tggggagcta catcgcgccg aaatatacaa
    15181 cgaccgtaca ggtctatgac tctttaggga aggcctacaa tctgagtatc gagtttcaaa
    15241 agatctccac gaacaaatgg gcgtggcggg cgattgaaga gtcggggatg gtggtttcct
    15301 tcgtagacga caaagaacgc gtttacacgc cgaatccggt cgacgccacc tcgatcgtcg
    15361 gcggaatcgt cgagttcgac tccggcggta cggtgagcgc cttcaaagcc atccggcaaa
    15421 atggacagat cgtggaagac gtgagcatca cgaggatcaa gttcgacccg ggagtcttcg
    15481 tagacgacgg ggcggctccc cctcccgaag agggagcgac ggctgtcacg gtgcagattg
    15541 acttttccga gctcgtccag ttcgcgggcg ccttttccgc ggccgtctcc gaacaagatg
    15601 ggaacgctat gggcgtgatg cagaacttcg cgatcaacgt cagcggacag gtgatcggca
    15661 ccttttccaa cgggaaaagc gaggccttgg gtcagatcgc tctcgcgatc ttcaataacc
    15721 cgggcggtct tcaagacctg gggaacaccc tgttcacggt cacgccgaac agcggtgtcc
    15781 cgatgatcgg aatcgccgat gtgggcggtc gcgggacgat cagcccgggg gttttggaga
    15841 tgtcgaacac cgacctctcc gaagagttca ccgggatggt catcgtccag cgcgccttcc
    15901 aagccaacgc ccgaacgatc acgacttcgg acgaaatcct tcaggagctc gtcaacctga
    15961 aacggtaagg acgcttactc gtccttgtta aggaataaaa aagggcggtg tatccgccct
    16021 ttttcattct cacttatctc ggccggaacc gaaaaccacg gccgtctttt tggggctcca
    16081 gtccggtaac gggcctcggc ttccttcccg cgc
//
LOCUS       DAOPXN010000037        15997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3728_length_15997_cov_5.807176, whole genome shotgun sequence.
ACCESSION   DAOPXN010000037 DAOPXN010000000
VERSION     DAOPXN010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3728_length_15997_cov_5.807176"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="PKM49_03395"
     CDS             <1..369
                     /locus_tag="PKM49_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNY81892.1"
                     /translation="GAARNIEEGGSLTIIATALVDTGSKMDDVIFEEFKGTGNMELIL
                     SRQLADRRIFPAINLQLSSTRREELLLSPEELRKVWILRKMLSTMGEEEGLTLILKKM
                     KETSTNEFFLTLLDKERSRY"
     gene            413..499
                     /locus_tag="PKM49_03400"
     tRNA            413..499
                     /locus_tag="PKM49_03400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:447..449,aa:Leu,seq:cag)
     gene            557..853
                     /locus_tag="PKM49_03405"
     CDS             557..853
                     /locus_tag="PKM49_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNY81893.1"
                     /translation="MPIYRYRCDQCEKETEVFQGINDEPLMTCEDCGGRLRKLIGRVG
                     IKFNGSGYYVTDAKKSNFSSSVDTKTETKETKKTAEPKRAPEAAKADPVKKEAV"
     gene            complement(1147..1221)
                     /locus_tag="PKM49_03410"
     tRNA            complement(1147..1221)
                     /locus_tag="PKM49_03410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1187..1189),aa:Gly,seq:ccc)
     gene            complement(1303..2235)
                     /locus_tag="PKM49_03415"
     CDS             complement(1303..2235)
                     /locus_tag="PKM49_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HNY81894.1"
                     /translation="MSATEREINRAPALLNQTPRMKASYDKGRRYLFVGCGSSYNLGY
                     TLSSVLNRNGIPSFVRSAGSVLAVHNLPDLNPETDIAVLISRTGESTETVLALEHLRK
                     IGLSTIGVTCQRDSSIVKKSERAYLFDFAYEESVVMTGSFVAILDALLGPFQPTNLSE
                     RATVVLEDSKRAIARLDLTHLNHFVFLGFNELLGLSKEGALKLQEMALESVDYSEPLE
                     YRHGPKSTLTASSLVVLQSHDTTYEKDLIAELKRYGAQVLVVGKTGDIIVGSSESDEL
                     PLRVLPVLWLGYQKAISKGLNPDQPKNLSKTVVI"
     gene            3014..4525
                     /gene="thrC"
                     /locus_tag="PKM49_03420"
     CDS             3014..4525
                     /gene="thrC"
                     /locus_tag="PKM49_03420"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNY81895.1"
                     /translation="MRAQNTRDASQSIPLVEALFSGTASNGGLWVPETFPHFSLLRAK
                     GDRPRFLDIGPDILRTFLPEAPMEWLSELFEGLYPTRFDHPEITPLLECGGSAGLART
                     YFLELFHGPTLAFKDLALTLLPHFMRYALRVSGKTGRLLVLTATSGDTGKAALSAFQS
                     IPDIQIFVLYPEGGISSIQRAMMLEEQATNTRVFALKGDFDDAQRLVKSLMNDLEWRE
                     ELNRRGIVLSTANSINLGRILVQVIYYFSSYQDIVDRGTIKLGDRVNYCVPTGNFGNI
                     LAGYYAYRMGLPIGRLVCASNRNRILNDFFRTGVYDLTAHSPLVKTLSPSMDILVSSN
                     LERWIFEVLDREGEKTAELMTSLTRCGRFSIDVSAKKDGDLFFSATCNDLESLETIRE
                     TFRDGALLIDPHTAVAVYALGQYRKATGDDTPCVVHSTASPFKFPEAMLRALQRDTGS
                     IDDHTRLDFLSEISGRPLPEAVRRLRLVRGREPESGNPEEIRQRLRQYAFEGW"
     gene            4522..5439
                     /gene="thrB"
                     /locus_tag="PKM49_03425"
     CDS             4522..5439
                     /gene="thrB"
                     /locus_tag="PKM49_03425"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNY81896.1"
                     /translation="MIGISQRVEVVVPATSANLGPGFDCLGIALTIVNRYTFLLRDEG
                     IEISGCPEEFCNADHMTVRAFHSLYKEVGQTPPGLRLHLEGRIPIARGLGSSAACILA
                     GLAAANRFLGDRFSEEELFERACRLEGHPDNIAPALFGGFCAALRDDRTDRFSYRKFT
                     VHQFPRLYALVPDFCLSTAQARSVLPGLYTKEDVVATIQRLAWLLGNLTQHDLKSFAI
                     GFEDRVHHPYRGPLIPDWEPITRHLSQKIDGGVTISGSGPTLLVWVHGSAQPIATYIR
                     ELAPLLQARWEVLPCALNSEGLRVVSSVE"
     gene            complement(5478..6365)
                     /locus_tag="PKM49_03430"
     CDS             complement(5478..6365)
                     /locus_tag="PKM49_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY81897.1"
                     /translation="MLTERGIFLERAASGELRGLAERLSQVLTHCELCPRRCRVDRTR
                     HIGYCRQSAAVRLTNAVVHTGEEPPLIVGAGSGAVFFSGCTLGCVYCQNFGISQQNRG
                     FDYTDEALAEIFLSLQRAGVSNLNLVSPTPHLRAIVSALDIGARRGLSLPVVYNTSSY
                     DNVETLRWMDGIVDLFLADIRYTSEEYGLKYSGVKDYWSVTQKALREMFRQVGSKGMI
                     IRLLVLPDGINGTREALRFVSEELSTKVYISLMSQYFPVYKALKMPPLNRRITAREYE
                     EARGFLEEFGFENGWCQDF"
     gene            complement(6374..7321)
                     /locus_tag="PKM49_03435"
     CDS             complement(6374..7321)
                     /locus_tag="PKM49_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNY81898.1"
                     /translation="MRLSIFLFLCAVLFVCPSCTTTDTVRFDLYFLNASIRERTLGFI
                     EGATVSLDLCVYDISDPEVLRALAGKAAQGVAVRVVTEADNLAELGLLTRELEVVVDD
                     DRGLMHCKYMIADGKQVWGGSTNLTKTSLDNHYNDIFIASDPFIVRRFQDHFEHCMNG
                     LFKSDRPSAKEKGPVYFSPEDLPFNALMNLLSSAKEEVLIGIYAFSDYRIAHFLKVLS
                     AHGVEIYVFADRGWNQGSPYSQSVEVDQYTLLRYDLLETGLMHQKFIVVDRSAVLFGT
                     YNFTASAETKNDEYLILSREASVVERFRQRFFELWKASE"
     gene            complement(7372..7448)
                     /locus_tag="PKM49_03440"
     tRNA            complement(7372..7448)
                     /locus_tag="PKM49_03440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7412..7414),aa:Arg,seq:gcg)
     gene            complement(7453..7542)
                     /locus_tag="PKM49_03445"
     tRNA            complement(7453..7542)
                     /locus_tag="PKM49_03445"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7506..7508),aa:Ser,seq:gct)
     gene            7866..9512
                     /locus_tag="PKM49_03450"
     CDS             7866..9512
                     /locus_tag="PKM49_03450"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HNY81899.1"
                     /translation="MEDIIYVVGHKNPDTDSICAAIAYADLKKRSGVGVRAVPARIGE
                     INRETEFVLNTFKLPVPMFLETLKLQVQDLQLDPAVTISSEISVKTAWSLMQKHNAKT
                     LPVIDDNGHLIGIVTLSDITSKYMDMVDTNLLGISDTPLQNVLETINARLVIGHVTDF
                     SPTGKILIAAMTPDRMAHWVEKNDVVITGNRLENQVEALKLGANCLIITGGAVEETPE
                     LQRLVEEKKSLVLVTPFDTFTTARLIPQSIPVKYLMTSRNLVAFNRDDFLDDIKDSML
                     STRYRSYPVVDDENRVHGVVARYHLISQRKKKVILVDHNERSQCISTIDEAEILEIID
                     HHRVGDIQTGKPIFFKNEPLGSTSTIIGNLFFENGIRPSRSIAGILCSAIISDTLKFR
                     SPTCTYTDIRMAERLAELAGIKDIDEYAQKMFQAGSVLVGKTPEEIFTRDFKAYELGR
                     YKIGISQINVFGTEFSAEMKKEVLGFMEGYCRAKGFDLLILMLTDMMKENTELLFTGE
                     SRELVSLAFEAQPKGNTLFLPGTLSRKKQVVPLLSAAAQS"
     gene            9526..10551
                     /locus_tag="PKM49_03455"
     CDS             9526..10551
                     /locus_tag="PKM49_03455"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015136669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNY81900.1"
                     /translation="MGEKIKVGLLFGGPSPEHKISIESARMVFYSLKTDHNREKYAIY
                     PFYISESGYFNQFNQMRYASPGIPPEKNVAQRAIPSSLWLMDVIFPVMHGKFGEDATI
                     QGFLSTLQLDFVGSDVMASALAMDKIGMKALLASGGFLIPRYKVILRGESPNWKEIAD
                     TLRFPLFIKPSNSGSSIGVTKVKDASGLESAYREALKWMDRVIVEEGVSCRELECGVL
                     GDTDPIVSPVGEVSYTGEFYDYATKYGTGGATSRLIIPADIPVDVAERVQRMSLEIFK
                     LFHARDMARVDFFYVERTGEVLVNEINTVPGFTKSSMYPKLWEKLGILNWDLSDRLIR
                     LALNRKK"
     gene            10580..11383
                     /locus_tag="PKM49_03460"
     CDS             10580..11383
                     /locus_tag="PKM49_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81901.1"
                     /translation="MKNRSSEKKVGVVFLLMWIAIALFYLTWDGWLYEWQSCGYQGTN
                     QHFDLDLFDLKIQRNADGTIQYLSTNNLHNLIYICYFVVFLGAMGLITDRKWMKQGAI
                     AIYPISVVALISTLFPLSNHAYVVQIAYDAVHVISIVLGLYCLSKEEIRVREMFFGIF
                     GTWFFYLLARILLEPWPFWQTNRDAYFSLNQINDMPFYFYGLEYLPVVAILFGINWLF
                     SKTQRKEPVLFWKMIFPLVCSIIIGFILIAAGMIQLQQLDMTANCPLPH"
     gene            complement(11533..12009)
                     /locus_tag="PKM49_03465"
     CDS             complement(11533..12009)
                     /locus_tag="PKM49_03465"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNY81902.1"
                     /translation="MYQYDTYCGLYCGACTIKKANEDGTLNELAREWEEDPNSLVCEG
                     CKGKVTCHYCIGCPIQLCNREKGLEHCGQCPDFPCERLRAFDADKYAHHTGVIRNLVA
                     KREISTEEWLEGQAKRWRCEKCGARFTWYDEFCLNCGAPLYNSVKEEEYIKIARPK"
     gene            12259..14898
                     /gene="ppdK"
                     /locus_tag="PKM49_03470"
     CDS             12259..14898
                     /gene="ppdK"
                     /locus_tag="PKM49_03470"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HNY81903.1"
                     /translation="MSKKWVYFLGKSKAEGGAGMKEILGGKGANLAEMINIGIPVPPG
                     FTISTEVCKTFYDLGKRYPEELKDQVEAAMKMLEKETGKRLGDLVNPLLVSVRSGAAI
                     SMPGMMDTILNLGLNDRTVEALAKGTGNARFAYDSYRRFMQMFGDVVLTIPHEDFEKE
                     LEAVKAQKGVKQDTDLNADDLKEVVKRYKAVYQKNGKEFPQDPQQQLWASIGAVIQSW
                     MNERAITYRRLNNVQEGSLLGTAVNIVSMVYGNMGERSGTGVAFTRNPSNGEKRYYGE
                     YLKDAQGEDVVAGIRTPLQLDQMANEWPEVYAQLTRTFDILEKHYKDIQDVEFTIENQ
                     KLYMLQTRNAKRTAAAAVKIAVDMVEEGVIDKKTALKRVTPEQIENLLHPTFDLKEKK
                     QARVLTVALPASPGAACGRAVFEAKVAEQWAKKGEKVILVRVETSPEDIGGMAAAQGI
                     LTARGGMTSHAAVVARGMGEPAVVGAEELEVEYEKKCFVVNGTRVNEGDWLSLDGTTG
                     EVLLGKVRTIKPEGLSGPLAKLLGWADEVRKLGIRTNADIPRDATVAREFGAEGIGLS
                     RTEHMFFEADRLPIVRRMIVGRTLKDRQKALDELEPIQKKDFYGIFKAMHDLPVTIRL
                     IDPPLHEFLPQDHESQLKAAQELNITLEELKMTVEELHEFNPMLGHRGVRLTVTYPEI
                     AVMQTRAIISAAIDLKKEGVVTKPEIMVPLVGMKEELKMLHDIIKREADELIKAAGVD
                     LPYLIGTMIEVPRGALTADEIAEYAEFFSFGTNDLTQMTYGFSRDDAGKFIKDYLERG
                     ILAFDPFKRIDRDGVGQLIRMATQKGRSVRPNLKIGICGEHGGEPNSIEFCHIVGMNY
                     VSCSPYRVPVARLAAAHAALANG"
     gene            complement(15381..>15997)
                     /locus_tag="PKM49_03475"
     CDS             complement(15381..>15997)
                     /locus_tag="PKM49_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81904.1"
                     /translation="QMIKDLNLDMTDSELETKLQKLIKADLISSGSTAFRYAVAKDKT
                     YELVFRRLYQDEIDNFIPDIRKELRQEMGRTSYEKGKFREYLVRERIKKPFNLKDLAE
                     NGIDLTIKPKTILERETVKVGLRDREIDLIVKGNVEMWIDVKDTEGKYGKREADRWLE
                     IKQSISEKSPKTLFATYSQNGYTVSAKELLVSSGVYVFKGEEGQ"
BASE COUNT         3782 a         4263 c         4336 g         3616 t
ORIGIN      
        1 ggagccgctc gtaacataga ggaaggcggc agtttgacga ttatcgccac cgctttggtc
       61 gataccggaa gcaagatgga cgatgtcatc ttcgaagagt ttaagggcac cgggaatatg
      121 gagctgatcc tctcacgaca gttagcggac cgccgcatct ttccagccat caacctccag
      181 ttgtccagca cccgaagaga agaactcctc ctctccccgg aagaattgcg caaggtatgg
      241 atattgcgga aaatgctgtc caccatggga gaagaggaag gcctgaccct catcttgaag
      301 aagatgaaag agaccagcac gaatgagttt ttcctcacgc tcctcgacaa agaaaggagt
      361 cgttattaac acttgacaaa atcggaagga tcaggtagaa taacgacgct cagccgagat
      421 ggcggaattg gcagacgcgt atgattcagg ttcatatggg agtattcctt tgcgggttca
      481 agtcccgctc tcggcaccag atgtccggaa acggacgaag aggataacaa tgtaatagga
      541 ttgaggagga aaaactgtgc ccatctaccg ttacagatgc gatcaatgcg aaaaagaaac
      601 cgaagtcttc caaggaatca atgacgagcc gcttatgact tgcgaagact gtggcggtag
      661 gctccgaaaa ctgatcggcc gggtcggcat caagttcaac ggatccggtt attacgtcac
      721 cgacgcgaag aagtccaact tctcctcttc ggtggacacg aagaccgaaa ctaaagagac
      781 gaaaaagacc gccgaaccga aacgcgcgcc cgaggcggcc aaagcggacc cggtgaagaa
      841 ggaagccgtt taaccgaacg gctgcccggt ctctttattt cacccgactc tttttcatag
      901 cacatcgcga ctcctggaaa aagaagcctt ctggcttctt tttcgttttt catagcgtgc
      961 tttggaagag aagtcggggc acgatcgaga cacccgaaaa cgtatcgtaa gaagccgaga
     1021 gagtcaggta gaaaaaaagc ttccgggcct aaagaaccga aggcccggaa gcttttcaca
     1081 acaccgtctg ttatgaaccc caccctacag agatcggggc atcctaatgg atggctgttc
     1141 gttttttgga gcggtcgacg ggactcgaac ccgcaacacc cagcttggga agctggtgct
     1201 ctaccattga actacgaccg cgaacgctcc ccctattcta taccaatcgc ttttttttgt
     1261 caagcggaga agggccatcg agggctctgg catttcggtc gatcatatga cgacggtttt
     1321 cgacaggttt ttcggctgat cggggttcaa ccccttcgaa atcgctttct ggtagcccag
     1381 ccagagaacg ggaagaaccc gtaaggggag ttcgtcggac tcggacgacc cgacgatgat
     1441 gtcccccgtc tttcccacca cgagcacttg cgcgccgtat cttttcaact ctgcgatgag
     1501 atccttctca taagtggtgt cgtgagattg caacaccacc aggctggaag cggtgagcgt
     1561 cgatttgggt ccgtgccggt actccagcgg ttcgctgtag tcgaccgatt ccaacgccat
     1621 ctcttgcagc ttcagcgccc cttctttgga gagcccgagc agctcgttga aacccaagaa
     1681 cacgaaatgg ttcagatgcg tcaaatccag ccgcgcgatc gcgcgtttcg aatcctccaa
     1741 gacaaccgtc gcccgttcgg ataggttggt gggttgaaat ggccctaaaa gcgcgtccaa
     1801 aatcgcgacg aacgaccccg tcatcacgac gctttcttcg taagcgaagt cgaacaagta
     1861 cgcccgctcg ctttttttca cgatagaact gtccctttga caggtgacgc cgatcgtgga
     1921 cagcccgatt tttcgcaggt gctccagcgc taagaccgtt tctgtcgatt cgcccgttcg
     1981 ggatatcagt acggcgatat cggtttcggg attcaggtcc ggcaggttgt gcacggccaa
     2041 gacgctgccg gccgatcgaa cgaagctcgg gatgccgttt cgattgagaa ccgacgatag
     2101 ggtgtacccc aggttgtaag aagacccgca cccgacgaac agataccgtc tccccttgtc
     2161 gtaagacgcc ttcatccggg gcgtttgatt gagtaaagcc ggcgctcggt tgatctctct
     2221 ctctgttgcg ctcacttgac ccctccattc tcttcgagaa gtcggcgcct gttagacctt
     2281 gtggaaagca tttccgcctt ggcgccgttc gacgcaatca taacatcttt tatcgattgt
     2341 tgtcaacgaa tcgtgccgtt acacccgtgt tcttataggt gtgggtcaag ttttgaggtg
     2401 taatcaagaa acttggccag aatcaatcgc gcaatttttt agcctaggta gtcatcgctg
     2461 cgtaagtgcc ccttctctcc gtagggccga cttccagtcg gcctttctca gggaacgtct
     2521 gggaacgccg aacttcagtt cggcggaatt tctgggaacg ccaactttaa agttggcata
     2581 tcttaaaaac aggattcgca cggagggcgc ataaaacaca gtgaaaagat acttgtgaac
     2641 gccctgggaa cgccaaactt tagtttggca ggtctactgg gagcgccaac tttcaagttg
     2701 gcatgtcttg gaaaaaaact cacacagaga ccacggaggt cacaaagaag agatgtgtta
     2761 tcgagagtca gcaccatgct gaagttttct ctgtgttctc cgcgtgctct gtgcgaaacc
     2821 attttttatt tttctgaaca aaccgaacaa aagttcgcag tgcccgggcg ctgcatcgcg
     2881 gcgtaaaaag gacaaaaggc ggatctcctc ctctttcttt tatttgttgc gtctcgacat
     2941 tacacccgcg ctcttatagc gcaaaaactt gaaaagcggt ataatcggcc atcaaaggtt
     3001 ggaggagaaa cgtatgcgag cgcagaacac acgagacgcg tcacagtcca tcccgctcgt
     3061 cgaagcgttg ttttcgggga ccgcttcgaa cggagggctg tgggttccgg agacattccc
     3121 gcacttctcc cttctacgag caaagggaga tcggccgcgg tttttggata tcggccctga
     3181 catactacgg acgtttttac cggaagcgcc gatggaatgg ctttcggaac tcttcgaggg
     3241 gctatacccc acacggttcg accacccgga gatcactccg ctattagagt gcggcggctc
     3301 ggcgggactt gcccgcacct atttcttgga actctttcac ggtccgacgt tggccttcaa
     3361 agacttggcc ttaacactcc tgccccactt catgaggtac gccttgcgcg tgtccggaaa
     3421 gaccggccga ttacttgtgc tgacggctac gtcaggcgat acgggaaaag ccgcgttatc
     3481 ggccttccag tctatccccg atatccaaat cttcgttctg tatccggagg gcggcatctc
     3541 gtccatccag cgagccatga tgctagaaga gcaggcgacg aacacgcgcg tcttcgcgtt
     3601 gaaaggggat ttcgacgacg cgcagcgatt ggtcaagtcg ctgatgaacg atctcgagtg
     3661 gagagaagag ctaaaccgac ggggaatagt cttatccacc gcgaattcga tcaacttggg
     3721 gagaatcttg gtccaggtga tctattactt ctcttcctac caggatatcg ttgatcgagg
     3781 gactatcaaa ctcggcgacc gcgtgaacta ctgcgtcccc accgggaact tcggcaatat
     3841 cctggccggc tactacgcct atcgtatggg gttgccgatc ggtcgcttgg tctgcgcgtc
     3901 gaaccggaac cgaatcctaa acgatttttt ccgtaccggc gtttacgatc tcacagcgca
     3961 ctcaccgctc gtgaaaaccc tctcgccctc gatggacatt ttggtctcat ccaatttgga
     4021 acgttggatt ttcgaggtcc tcgacagaga aggcgaaaaa accgcggaat tgatgacgtc
     4081 cttaacgagg tgcggccgct tttcgataga tgtgtccgcg aagaaagacg gagacctttt
     4141 cttttcggcc acttgcaacg acctggagag cttggaaacc attcgggaaa ccttccgcga
     4201 tggcgccctg ctcatcgatc cgcatacggc cgtcgcggtc tatgcgcttg gccaataccg
     4261 gaaagcgacc ggagacgata ccccttgcgt cgtccattcc acggccagtc ccttcaagtt
     4321 cccagaggcg atgctccggg ctttacaacg ggatacggga tcgatcgacg atcacactcg
     4381 gctggacttc ttgagcgaaa tctccggccg gccccttccg gaggccgtcc gacggttacg
     4441 attggtgagg ggcagagaac ctgaatccgg gaatcctgaa gagatccggc agcgacttcg
     4501 ccaatacgcc ttcgaagggt ggtgatcggt atctctcaac gtgtcgaagt ggtggttccc
     4561 gcgacgagcg ccaatttggg cccggggttc gattgcctcg gaatcgcctt aaccatcgta
     4621 aaccggtaca cctttctctt gcgcgatgag gggatagaga tttccggttg cccggaagag
     4681 ttctgtaacg cggaccatat gacggtccgt gcctttcatt cactctataa agaagtgggg
     4741 cagacgccgc ctggccttcg cttgcatctg gaagggcgga tcccgatcgc gcgcggtctc
     4801 ggtagcagcg ccgcctgtat cttggcgggg ctggccgcgg cgaatcgctt tttgggggat
     4861 cgcttttccg aagaggagtt gttcgaacgc gcctgccgac tggaagggca tccggacaat
     4921 attgcgccgg cgctgttcgg agggttttgc gccgcgctgc gcgatgatcg cacggatcgt
     4981 ttctcgtaca ggaagttcac ggtccatcaa ttcccacgcc tgtatgcgct cgttcccgat
     5041 ttctgcctgt cgaccgcgca agcccgatcc gttctgcccg gcctttacac gaaagaggat
     5101 gtggtagcga caatccaacg cctggcctgg cttttgggga atctgaccca acacgatctc
     5161 aaaagcttcg cgatcggctt cgaagaccgc gttcaccatc cttatcgcgg ccctttgatt
     5221 cccgattggg agccgatcac gcgacattta tcccaaaaaa tcgacggtgg cgtcaccatc
     5281 agcgggtcgg ggccgaccct tctggtatgg gttcacggtt cagctcagcc catcgccact
     5341 tacatccggg aactcgctcc cctcttgcag gcgaggtggg aggtcctccc ctgcgctctg
     5401 aactccgaag ggcttcgggt tgtttctagt gtagaataga tttcgttatg ggcttccccc
     5461 acttggacgg acaagggtta gaagtcctga caccaaccgt tttcgaagcc gaattcttcg
     5521 agaaagccgc gcgcctcttc atactcccga gcggtgatcc ttcggttcaa gggaggcatt
     5581 ttgagcgctt tgtacacggg gaagtactgg gacatcagcg atatatacac ctttgtagat
     5641 agttcttcgg aaacgaagcg cagcgcttcc ctggtcccat tgatcccatc ggggagaacg
     5701 agcagccgga tgatcatacc tttcgagccc acttggcgaa acatctcccg caacgctttt
     5761 tgagtgacgg accaataatc cttgaccccg gaatacttca agccgtattc ctcggacgtg
     5821 tagcgtatgt cggcaaggaa aaggtccacg atcccgtcca tccagcggag cgtttcgacg
     5881 ttgtcgtagc ttgaagtgtt gtagacgaca ggcagggaaa gaccccgacg cgcgccgatg
     5941 tccaaggcgc tgacgatcgc tctcaagtgg ggggtgggtg agacgaggtt tagattggag
     6001 accccagccc tttgaaggga cagaaagatc tccgccaagg cttcatcggt gtaatcgaat
     6061 ccccgattct gctggctgat tccgaagttt tggcagtaga cacagcccaa tgtgcagcca
     6121 gaaaagaaga cggctccgga gccggcgcca acgatgagcg ggggctcttc acccgtatgg
     6181 acgaccgcgt tggtcaatct caccgccgcg ctttgcctgc aataaccgat gtggcgcgtg
     6241 cgatcaaccc gacaacggcg cgggcacaat tcgcaatggg tgagaacctg cgacaggcgt
     6301 tccgcaagac cgcgtagctc tcccgaagcc gccctttcca agaaaatacc gcgttccgtc
     6361 agcatagccc ctcctattcg gaagctttcc aaagttcgaa gaaccgttgc cgaaaacgct
     6421 cgacaacgga ggcttctcgt gaaaggatca ggtattcgtc gttcttcgtc tccgcggagg
     6481 cagtgaagtt ataggtgccg aacaatacgg cggatcggtc cacgacgatg aacttttgat
     6541 gcatcagtcc cgtttccaag agatcatatc tcaaaagagt gtactgatcg acctcgacgc
     6601 tttgactgta agggcttccc tgattccatc cacgatcggc aaaaacgtaa atctccacgc
     6661 cgtgcgccga caggaccttg agaaaatggg ctatacggta gtcgctaaac gcatagatcc
     6721 cgatcaacac ctcttctttc gcgctcgaca acaggttcat caaagcatta aagggaaggt
     6781 cctcagggga gaagtagacg ggtccctttt ctttcgcaga cgggcggtcg cttttgaaca
     6841 gaccgttcat acagtgttcg aaatggtcct gaaatcgccg gacgatgaag gggtcggacg
     6901 cgatgaagat gtcgttgtag tggttgtcta aactggtctt cgtcaggttg gtggatcctc
     6961 cccacacctg ttttccatct gcgatcatat atttacagtg catcaagccg cggtcatcgt
     7021 cgactacgac ctctaactcc cgagtaagca gccctaactc cgcgaggttg tccgcttccg
     7081 tgacgacccg caccgcgact ccctgcgccg cctttcccgc caaggcgcgc aagacctccg
     7141 gatcgctgat atcgtacacg cataaatcca aagaaaccgt cgctccctcg atgaaaccga
     7201 gggtgcgttc cctgatgctc gcgtttagaa agtaaaggtc gaagcgtacg gtgtccgtcg
     7261 ttgtgcacga agggcaaacg aagaggacgg cgcaaaggaa taaaaaaata gagagcctca
     7321 tacgccctct tctttcgatc gtttcatttc tatcccactc cctgatagaa ttggtgcgct
     7381 cggggagatt cgaactccca acctccacat ccgcagtgtg acactctttc caattgagct
     7441 acgagcgcga catggcggag agggagggat tcgaaccctc ggagggcaaa caccctcact
     7501 tgcttagcag gcaagcacct taggccgctc ggccacctct ccaaaattgc tcgatagtat
     7561 accataaact tttcgccaat tcaagcaaag aatacgcccc gactcatccg gaaagggttc
     7621 gaaacgccgt aaggcgagcc ctcgggtcgt ccgcgggggt atcagccggg tcccatgttg
     7681 cttgatagtc gtggtttcta gacagaaaca ccttacgggc atagcggctt cgggtagagg
     7741 taggcttttg tgttgacgga ggtcgaagtc actcaagaga ccggcggtat aaaacttcga
     7801 gaaagccgga aaatccatcc cttatgctat aatttcaccg tccattttaa aaggtgggat
     7861 gccgattgga agatatcata tatgtggtgg ggcataaaaa tccggatacg gactctattt
     7921 gcgcggcgat cgcttacgcg gatttaaaaa agcgttcggg ggtcggtgtt cgcgctgtcc
     7981 ccgcaaggat tggagagatc aaccgggaaa cggagttcgt gctcaacacg tttaagctcc
     8041 cggttcctat gtttttggag accttgaagc tgcaagtgca agaccttcag ttggatccgg
     8101 cggtcacgat ctcttcggaa atctccgtca aaaccgcctg gagcctgatg caaaagcaca
     8161 acgccaaaac gctcccggtg atcgatgaca acggacattt gatcgggatc gtcacgttat
     8221 ccgatatcac gagcaaatat atggatatgg ttgacaccaa tttgctcggg atcagcgata
     8281 cgcctctgca aaacgtgctg gaaacgatca acgcgcgctt ggtgatcggt cacgtcacgg
     8341 acttttcgcc gacgggaaag atcctgatcg cggctatgac gcccgatcgc atggcacatt
     8401 gggtggaaaa aaacgatgtc gtgatcacag gcaatagact ggaaaaccag gtggaggcgt
     8461 tgaagttagg ggccaattgc ctgatcatca caggaggcgc cgttgaagag acgcctgagc
     8521 tgcagcgttt ggtagaggag aagaaaagcc ttgttctggt caccccgttc gacaccttca
     8581 ccacggcgag actcattcct cagagcattc cggtgaagta cctgatgacc tctcggaacc
     8641 tcgtcgcgtt caatcgggac gactttttgg acgacatcaa agactcgatg ctgagcaccc
     8701 gataccggag ctaccctgtc gtggacgatg aaaaccgcgt gcacggtgtg gtcgcgcggt
     8761 atcacctcat ctctcagcga aagaaaaaag tgatcctggt ggaccacaac gaacgttccc
     8821 aatgcatcag cacgatcgat gaagccgaaa tcctggagat catcgaccac caccgcgtag
     8881 gggatatcca gaccgggaag ccgatttttt tcaagaacga acccttgggg agcacctcta
     8941 cgatcatcgg aaacctcttt ttcgaaaacg ggatccggcc ctctcgctcg atagcgggga
     9001 tcctatgttc ggcgattatt tcggacacgt tgaagttcag gtcgcccact tgcacctaca
     9061 ccgatatccg tatggcggag cgactggctg agcttgcagg gatcaaagac atcgacgaat
     9121 acgcccagaa gatgttccaa gcgggctccg tactcgtggg gaagaccccc gaagagatat
     9181 tcacccggga ttttaaggcc tatgagctgg ggagatacaa gatcggcatc agccagatca
     9241 acgtcttcgg aaccgagttc agcgctgaaa tgaaaaagga agtcctcggg tttatggaag
     9301 gatactgccg ggcgaagggc ttcgatctgc tcatcctgat gctaaccgac atgatgaaag
     9361 agaataccga gcttctattc accggggaga gccgagagct cgtgtccctc gcctttgaag
     9421 cgcagccgaa gggtaacacg ctctttcttc ccgggacgct ctcccgtaag aaacaggtgg
     9481 ttcccttatt gtctgcagcg gcgcaatcgt aaaagggtgc gaggcatggg ggaaaagatt
     9541 aaggtagggc tgcttttcgg aggaccttcg ccggagcaca agatttcgat cgaatcggct
     9601 cgtatggtct tctattcctt gaaaaccgat cacaatcggg agaaatacgc gatttacccc
     9661 ttctatatca gcgaatccgg atacttcaac cagttcaatc agatgcgata cgcatcaccc
     9721 ggcatccctc ctgaaaaaaa cgtggcccaa cgggcgattc cttcctcttt gtggttgatg
     9781 gacgtcatct tccccgttat gcacggtaag ttcggcgaag acgcgaccat tcaaggtttc
     9841 ctctccactt tgcaactcga ttttgtcggt agcgatgtta tggcctccgc cttggcgatg
     9901 gacaagatcg gtatgaaagc cttgctcgcc agcggcgggt ttctgatacc gcggtataag
     9961 gtcattctac gaggagagtc cccgaactgg aaagagatag ctgacaccct tcgattcccg
    10021 cttttcatca agccctcgaa ctccggctca tcgataggcg tcaccaaggt aaaagacgca
    10081 tctgggttgg agtccgctta tagggaagct ttgaagtgga tggatcgggt catcgtggaa
    10141 gaaggggtat cgtgtcgaga gctggaatgc ggggtgttgg gcgatacaga cccgatcgtc
    10201 tcgccggtgg gtgaggtttc ctatacgggg gagttttatg actatgccac caaatacgga
    10261 accggcggcg cgacgagccg attgatcatc cctgccgata ttccggttga cgtcgctgaa
    10321 cgagtgcaac ggatgtcttt agaaatcttc aaactctttc acgcgaggga tatggcccgg
    10381 gtcgacttct tttatgtgga gcggaccgga gaggtgctgg tcaatgaaat caacacggtg
    10441 ccgggcttca caaaatctag catgtacccg aaactatggg aaaagttagg aattctgaac
    10501 tgggatcttt cggaccgact catacgtttg gcgttgaaca ggaagaagta gtgggcacaa
    10561 aaggaggaca atcttggctt tgaaaaaccg atcatcggaa aaaaaagtcg gtgtcgtttt
    10621 tttgctcatg tggatcgcga tagccctttt ctacctcacc tgggacggtt ggctgtacga
    10681 atggcaatcc tgtggctatc aaggaacgaa tcagcacttc gatttagatt tatttgacct
    10741 gaaaatccag aggaacgccg atggcaccat acaatacctt tcgaccaaca acctccataa
    10801 cctcatctat atctgctact ttgtcgtctt cttgggcgcg atggggttga tcaccgaccg
    10861 gaagtggatg aaacaggggg cgatcgccat ctaccctatt tcggtggtcg ccctgatttc
    10921 cacgctattc ccactgagca atcacgccta cgtagtgcaa atcgcttatg atgccgtgca
    10981 cgttatcagc atcgttttag ggctctattg cctcagcaag gaggagatac gggtgcggga
    11041 gatgttcttc ggtatattcg gcacctggtt tttctatctt ctggctcgca tcctcctcga
    11101 accgtggccc ttttggcaaa cgaaccggga tgcctacttc agcttgaatc agatcaacga
    11161 tatgcccttt tatttctacg gtttagagta cctcccggtt gtcgcgattc tatttgggat
    11221 caattggcta ttttctaaaa ctcagcgcaa agagccggta ctcttttgga aaatgatctt
    11281 cccgctggtc tgttccatca tcatcggttt cattttgatc gcagcgggga tgattcaatt
    11341 gcagcaactc gatatgaccg ccaactgtcc gcttccgcac taagacgcga cgttacgcgt
    11401 cagtaaaagc tcgccccttc cgaacgccgg cgtcctcaca tttcgtcgtt atctatcgtt
    11461 tgaagggccg aataagaagc ctgtgactct tccccgtcgg gtcaaatcgg ggtcagaatc
    11521 tgcgcgcggg tgttatttag ggcgcgcgat tttgatgtac tcttcttctt ttacgctatt
    11581 gtataacggc gcgccgcagt tcaaacagaa ctcgtcatac caagtgaacc gagcgccgca
    11641 tttttcacat cgccaacgtt tggcctgacc ttccagccat tcctctgttg aaatctctcg
    11701 tttggcaacg aggtttcgga tgacgccggt gtggtgcgcg tatttgtccg cgtcgaacgc
    11761 ccggagccgc tcgcagggaa agtccgggca ctgcccacaa tgttcgaggc ctttttcgcg
    11821 gttgcagagt tgtatcgggc aacctatgca ataatgacag gtcaccttcc ctttgcaccc
    11881 ttcgcacacc aatgaattcg ggtcttcttc ccattccctc gccaattcat taagagtgcc
    11941 gtcctcgttc gccttcttaa tcgtgcaagc gccacaatag aggccgcaat aggtgtcgta
    12001 ctggtacatg aaattactct cctttcggtt ttgcatagag acggtaagta ttatacgcct
    12061 atttcaagac tttcgcgctg ggatgccgct agtccttccc aaccgcaaac tgacgacagg
    12121 gaattgaggc cgttcgatga tacccgcccg cgcatacctt cgctaagacc cgtatacagt
    12181 cgaatccgtc agaaaacgcg gaatcatggt ataatagagc ccggtttttt atccaaattt
    12241 gaaggaggaa cccgaaaaat gagcaagaag tgggtttatt ttttaggaaa gagtaaagcg
    12301 gaaggcggcg ccggtatgaa agagatcctt gggggaaaag gtgccaacct tgcggagatg
    12361 atcaacatcg ggattcccgt cccccctggg tttacgatat ccacggaagt gtgtaaaacc
    12421 ttctacgatc tcgggaaacg ctatccggaa gaattgaagg atcaagtcga agccgctatg
    12481 aagatgctgg aaaaggaaac gggtaaaagg ttgggagacc tcgtcaaccc cttgctcgta
    12541 tccgtacgat cgggtgcggc gatctcgatg ccgggtatga tggacaccat cctcaacctc
    12601 ggtttgaatg accggacggt ggaggccctc gcgaaaggga caggcaacgc gcgcttcgcc
    12661 tatgactcgt atcgccggtt tatgcaaatg ttcggagatg tcgttttgac catacctcac
    12721 gaggatttcg aaaaggaact cgaggctgtg aaagctcaga aaggcgtcaa acaagatacg
    12781 gatctgaacg ccgatgacct taaagaagtg gtcaaacgat acaaagcggt ctatcaaaaa
    12841 aacgggaagg agtttccgca agacccgcaa cagcagcttt gggcgtctat aggggccgtg
    12901 attcagagct ggatgaacga acgcgccatc acctatcgaa ggctgaacaa cgttcaggaa
    12961 ggttctctgt tgggcaccgc cgtcaacatc gtctctatgg tctatgggaa tatgggcgag
    13021 agaagcggga cgggtgtggc gttcacacgg aacccatcca acggtgagaa acggtattac
    13081 ggagagtacc tgaaggatgc gcaaggagaa gacgtggtcg ccggaatccg gacgcctcta
    13141 caactcgacc agatggcgaa cgaatggccg gaggtctacg cacagttgac ccgaaccttc
    13201 gacatactcg agaaacatta caaagacatc caagacgtgg aattcacaat tgagaaccaa
    13261 aagctctata tgcttcaaac ccgtaatgcg aaacgaaccg ccgccgcggc cgtgaaaata
    13321 gcggtggata tggtcgaaga gggcgtgatc gacaagaaaa ccgcgttgaa gcgcgtcacg
    13381 cccgaacaga tcgaaaactt gctgcacccc acttttgact tgaaagaaaa gaagcaagcg
    13441 cgcgtattga ccgtcgcgct acctgcctct cccggcgcgg cttgcggtag agccgttttc
    13501 gaggcgaagg tggccgaaca gtgggcgaag aagggggaaa aggtcatcct cgttcgcgtg
    13561 gaaacctccc cggaagatat cggcgggatg gccgccgccc aaggtatcct gacggcccgc
    13621 ggcgggatga cctcccatgc ggcggttgtc gcgcgaggga tgggcgagcc cgccgtcgtg
    13681 ggagccgaag agctggaagt ggaatatgag aaaaagtgct ttgtggtcaa tggcacgcgg
    13741 gtgaatgaag gagattggct ctccttagac gggacaaccg gagaggtgct gctcggaaaa
    13801 gtgaggacca tcaagcccga aggactctca ggcccgctcg ctaaattgct ggggtgggcg
    13861 gacgaggtcc gtaaattagg catccggacg aacgccgata tcccgagaga cgcgacggta
    13921 gccagggagt ttggcgccga aggcatcgga ctgagtcgga cggagcatat gttcttcgaa
    13981 gcagatcggc tgccgatcgt ccgccgaatg atcgtgggcc gaacgctcaa ggatagacag
    14041 aaggcgttgg acgaactcga gccgatccaa aagaaagact tctacggcat ctttaaagcg
    14101 atgcacgatt tgcctgtcac gattcggctc atcgatcccc cgctccacga gttcctacct
    14161 caagatcatg aatcgcaact gaaggcggct caagagctca atatcacgct tgaagagttg
    14221 aagatgacgg tagaagagct gcatgagttc aacccgatgc tgggacatcg gggcgtccgt
    14281 ttgaccgtga cctatcccga gatcgcggtg atgcagactc gagcgatcat cagcgccgcc
    14341 atcgatctga aaaaagaagg cgtggtaacc aaacccgaaa taatggttcc gttagtcggg
    14401 atgaaagaag aattgaagat gcttcacgat atcatcaagc gcgaggcgga cgaactgatc
    14461 aaagcggccg gtgtggatct gccctacctc attggaacga tgatagaagt tccaagaggt
    14521 gctctgacgg ccgacgaaat cgccgaatac gctgaatttt tctctttcgg aacgaacgac
    14581 ctcacccaaa tgacctacgg attcagcaga gacgacgccg gcaagttcat caaggactac
    14641 cttgagcgag gcattctggc cttcgacccc ttcaagagaa tagaccgaga cggtgtgggc
    14701 cagctcatcc ggatggcgac gcaaaaaggg cgaagcgttc gcccgaacct caagatcggc
    14761 atttgcggag aacacggcgg agagccgaac tctatcgagt tctgccacat cgttgggatg
    14821 aactatgtca gctgctcccc gtaccgggta ccggtcgcgc gcttggccgc cgcccacgcg
    14881 gcattagcga acggataacg agagccaatt caaaaaaggc gcctacgggc gccttttttg
    14941 tttgcgtccc gattgccttt tgggtattat gcccttccgg aagcgctgtg tagtcatcgc
    15001 ggcgtaagcg ctccttcttt gcgcgacagc gcgataccct tgggaacgcc tgggaacgcc
    15061 gaactttagc gtggcaaggt ttttgtaaaa acaaaagaag tttcgcacag agaacacaga
    15121 gaacacggag aaaggaggca ttcccaagcg cggcatcgcg gcgtaagcgc cccttctttt
    15181 cgcgacggcg cgatacctcg acatcatggc ggcataaaga aaacaaaaag ggaggatcgc
    15241 ggggcccaaa gcgggcatcg cggcgtaagc gctccttctt tttgcggcgg cacgatgccc
    15301 cgacatcatc gcggcgtaaa gaaaacaaaa gggggatcgc ggggtcccgt tggttacccc
    15361 gcgattaccc caaggcgctc ctactgcccc tcttcccctt taaacacgta caccccactc
    15421 gatacgagga gctcttttgc ggatacggtg tacccgtttt gagagtacgt tgcgaataag
    15481 gttttagggc tcttctcgct gattgactgc ttgatctcta accagcggtc agcttcgcgt
    15541 ttcccgtact tcccttccgt atcttttacg tcgatccaca tttcgacgtt ccctttcacg
    15601 atcaggtcga tctctctgtc ccgtaacccg actttgaccg tttcgcgttc gagtatcgtt
    15661 ttgggtttga tggtgagatc gattccgttt tctgcgaggt ctttcaggtt gaacggtttc
    15721 ttgatccgtt ctcgcacgag gtattctctg aacttcccct tctcatagct cgttctcccc
    15781 atctcctggc ggagctcttt gcgtatgtct ggaatgaagt tgtcgatttc gtcctggtac
    15841 aatctccgaa ataccaactc ataggttttg tccttcgcga cggcgtatct gaatgcggta
    15901 ctccccgatg agatcaggtc tgctttgatt aacttctgca gcttcgtttc cagttcggag
    15961 tcggtcatat cgaggttcag gtctttgatc atctgcg
//
LOCUS       DAOPXN010000038        15954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3742_length_15954_cov_5.062583, whole genome shotgun sequence.
ACCESSION   DAOPXN010000038 DAOPXN010000000
VERSION     DAOPXN010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3742_length_15954_cov_5.062583"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKM49_03480"
     CDS             complement(<1..329)
                     /locus_tag="PKM49_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81905.1"
                     /translation="MKNIRMTMFFSILFFTLFGVVYAATNQIDLSEYGQYVINYEDRE
                     FADYLHKRLADFSQILDNTQKQLKSPYVFRTNLFSETGEISLIKADEKEKLNQYVNII
                     DSLAKKR"
     gene            complement(810..1289)
                     /locus_tag="PKM49_03485"
     CDS             complement(810..1289)
                     /locus_tag="PKM49_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNY81906.1"
                     /translation="MSVTFNPIGEVKFEDGNCFIVLKKEAIPATLGLDEYSHIQIVWW
                     FHLYDNEDTRKYYVLDKPYTKGPEKIGVFATRSPVRPNPIGITSCVLVKLDRAQNRLQ
                     VAGLDAENGTPILDIKPYEPSIDRVRDVKMPLWCAHWPAYVEDNATFDWRSEFNFPE"
     gene            1454..1975
                     /locus_tag="PKM49_03490"
     CDS             1454..1975
                     /locus_tag="PKM49_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNY81907.1"
                     /translation="MKTEVTRLSSILLGGVSFYGDPISAKGGWDTENEIGKTWQRFMT
                     FLRENPERPYSCGKQLFYEVHLYGSETKKKGYFEVFVGEEVNTATLPIALCAKYIPES
                     DYLKMTLFGREIVGDWWLKLETEILPALGFSAKSSHLIQVYDDRFKGMDNVDESILDV
                     FIPIEKNDEQGRI"
     gene            1956..2693
                     /locus_tag="PKM49_03495"
     CDS             1956..2693
                     /locus_tag="PKM49_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY81908.1"
                     /translation="MNKAVFDSVKKSIAYIEAHLFHDIAVRDVSDFVSYSQFYFSREF
                     SRYTRISIYDYIIKRKISESYKRLFKEKMKIIDLAFQYGFQSHEVYTRAFKKVFKENP
                     SEAVIYKPLAVYERIDDRYLEFVCELQVEEIDQPCEERIFEIAKPSDAQQTNDSLVQL
                     SHTNLFEIENVFYGRLTSIDQNCLSFNLNGLKAIARIHDTDTQCALRFFTDNYYDVAK
                     MKANYIVLRRANGYVDVLIPTAISTGD"
     gene            3171..3803
                     /locus_tag="PKM49_03500"
     CDS             3171..3803
                     /locus_tag="PKM49_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNY81909.1"
                     /translation="MDLRSLILEKALIVPAILIGFTVHEYAHAKVANLLGDDTPYYQG
                     RLTLNPLAHIDWIGFLALLLVGFGWAKPVQVNPRSFKNVRTDFLMVTVAGPLSNLVTA
                     FAFALLTAFLASFIRYAPNGTDLLFTLLKIIIHVNCILCVFNLLPIPPLDGYQILKTL
                     VPAQYGQFFYNLERYQFFFLFCLVLIPGASIIITVPARSLYFLLTGWIRF"
     gene            4078..5346
                     /locus_tag="PKM49_03505"
     CDS             4078..5346
                     /locus_tag="PKM49_03505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNY81910.1"
                     /translation="MRATLIIGDSRAMNEVKNGAVDLIVTSPPYWQIKDYGTVGQTGY
                     NQTLHEYFRDLYCVWAECYRVLKPGRRLCINIGDQFARSIVYGRYKIIPLHSECIAQC
                     ERIGFDYMGSIIWQKKTTMNPTGGATVMGSFPYPPNGMIEIDFEFILIFKKPGKGVKT
                     PAEIKEKSKLSKEEWKKYFSGHWSFGGAKQLEHQAMFPEELPKRLIKMYSFYGETVLD
                     PFLGSGTTMKAALTLNRHAIGYEINESFLPVIREKAGFSGERLMPAHTLTTIRQSMPK
                     EDTSAEMTYTPGIQDAKRKFGSKGLDLNKGREYFRVVSLEGPDTLVLENGLKVRLLGV
                     KTFPEKREALGQYLCKYVCKKKIYLQYDQKTPDSNSEGIVSAYVYLSNRLCLNLSVLK
                     AKLVHVDPSVDHPHKERFRRLAENARTKDG"
     gene            complement(5385..6119)
                     /locus_tag="PKM49_03510"
     CDS             complement(5385..6119)
                     /locus_tag="PKM49_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81911.1"
                     /translation="MHRNERFRRYASNVVRLLNTSEQRKQQIRREIILELSQQYKVYT
                     IDDPELILGREIDTAKAWQEKIDSGLVPLTNEYVSSVQVGSIPLVHITKTPNGVAKGI
                     IAIGPIAIGVIAIGGISVGVIGFGGIALSVLFSFGGLSLSAFLSFGSIAISGLLAIGG
                     LAFSYQTAIGGFCVAKEFAVGGVAHGKIAVGDIAKGLVAVYSQSGTGETLIHVSRGYT
                     EIRRQILKVAPDFPKWLLYIIRLILN"
     gene            complement(6268..6726)
                     /locus_tag="PKM49_03515"
     CDS             complement(6268..6726)
                     /locus_tag="PKM49_03515"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HNY81912.1"
                     /translation="MCIQDAKSQGKAGIAVVTSGKKKPFLTDKTFFQKKGFVTLDKAT
                     PYFELMALKLNNGPLPAFSPSAKNGTIPIQDGLALVYTNQCPFMEEYATLTAQRAREK
                     GFSVTLRKLESAAEAKELGSPFGTLGIYYNGAFQTHIPTSWDKLQAAIQG"
     gene            complement(6775..7020)
                     /locus_tag="PKM49_03520"
     CDS             complement(6775..7020)
                     /locus_tag="PKM49_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81913.1"
                     /translation="MEIIEITEQNIDKEHICCAIGKDKENENRAFTKKAWMKKNFRDG
                     LVFRRLDDRGKVFIEYLPIEKAWKPLIGANYLIINCL"
     gene            7811..9181
                     /locus_tag="PKM49_03525"
     CDS             7811..9181
                     /locus_tag="PKM49_03525"
                     /inference="COORDINATES: protein motif:HMM:NF021645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoPQ-activated protein PqaA family protein"
                     /protein_id="HNY81914.1"
                     /translation="MEKRARTKAIRRKFFTIFMLVLFLHSVIWSVSLSDILNKEPFTG
                     LPEVISESGLPNVDPNTYSGVVLKVLRLRTQQWLGADWEHTVLLAYNEELSPTMDTAL
                     VMIGGDYKYDREVLQAVISVLQESDGLFVFLFDVPNQPLLGGLREDWLISETFERYFQ
                     TLDPTVPALVPMVRSVRKVMDWVENAFGIRKFVLSGASKRGWTTFLTAAVDERVKGIV
                     PISFDFLNFPEQLRKQKEWYGAYSPEIAPYTEKDFPAKLEEDVGKELLKIVDPFQYIP
                     TLTTPKICVVGTNDPYWHLFSWQHYFPELSGYKQMLYIPNKGHGVELLRPILAILALI
                     DHVCCGTQLPEYQWKVDDRQIVVEWASQQDYTVLAAYLWTARSSTMDFRRAKWAISPL
                     PIPENQALAQIAEFELENVSAFVELLFETHEAGKAAKRRLYLSTPVFLFPKDSERSGG
                     IEIPRP"
     gene            9774..10517
                     /locus_tag="PKM49_03530"
     CDS             9774..10517
                     /locus_tag="PKM49_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNY81915.1"
                     /translation="MEAIKKIEGKRGTVIDEAWMELREMILSGMLAQGERLPSETDLA
                     HEMGISRNSLREAIGMLEREGLLLKKQGVGTFVTSVTPIIRGGIERLTGIMDFVKANG
                     YEAQSRLISFSASVANPTVSTLLNLSPGQVVYNLTTIKLASEVPVALCIDYIPESIFK
                     TPVKPERLQYSLFEGLEKEYGIEIFYAEAEIIPVISDRDIASKLQIPEGEAVLLLSQI
                     HFGKNNRKILYSKSYFPTGKFTFRLIRKR"
     gene            10565..11812
                     /locus_tag="PKM49_03535"
     CDS             10565..11812
                     /locus_tag="PKM49_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNY81916.1"
                     /translation="MRKAFLLGLFLLATLLSAATLEVLWMGWPKDQVMPLIEEFQKQN
                     PGVEIDIQLVPFGQLFQTIEVRLSSGGGTPDVYIVDGPNTASYAARGYLLPLDDFFST
                     QEKAAWFPSSIVAGSYRGKFYSVPYGTSSAGLFFNKEIFEKYGVPLPEESLDARMTWD
                     EVAELAKRLTIDTDNDGRPDIWGIVIEQIDRPYLLFPLAQSLGAQVLSPDGLTATGYI
                     TSEKFVEAATFYWKLFNEWKVAPQGVNDSAIAREYFGTGKAAMLLGNEWNIVRLLKYP
                     EIRFGLSPFPIFPGGTAVTPTGSWHVGINSKTKKQELALKFVKYITGKEAVIQWYLAN
                     GVAPARPDVYEALPEIFMHPMWQMFLQEMKTTAVTRPLTPGYSQYELILREAFNSIHY
                     GAPPKQALEDAAKKIDRELSKYK"
     gene            11987..12853
                     /locus_tag="PKM49_03540"
     CDS             11987..12853
                     /locus_tag="PKM49_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81917.1"
                     /translation="MFRSNRAGWFFILPAFVFLGLFKIVPIGVSVLESLRQTTYTGRS
                     YFVGFENYTYLFFEDPTFWNSFWITLIYSLVVNPLIILIALVMALLLNENHFFTKFFR
                     TVFFLPTAISFAVVSVIWSVVLDPHYGLANGFLTLFGLPAQPFFASTAQALWCLVFLN
                     VWRSAGYWMMYFLAGLQNIPESLYQAADIDGASAWTKTRKITLPLLSRTFAFVLVANT
                     AFNFLTFAPVYIITKGGPRGSTNLLMYESYKSAFVNLDMGRASAITTILLGIILIFSL
                     FEFKWTKAGFEY"
     gene            12864..13712
                     /locus_tag="PKM49_03545"
     CDS             12864..13712
                     /locus_tag="PKM49_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY81918.1"
                     /translation="MMRRYKKPLVLAAEFVLLTAISLFFILPILWIVASALRPSGPLY
                     TYANPLTLQTFFPTTVTFDNFVHIFKNLHFGRALLNSLLVAVATIAISVLICSMAGFG
                     LAKFAFRGKNLFFFIVLITFMVPFESIVIPLFVLVKRLNIDNTYFALILPAAGNGLAI
                     FLFRQFFSEVPTEILEAARIDGASWIRIFRKIILPLSIPAIVTVIVMVFMFQWNSLFW
                     PLIATHSSRFEVVQVAITAHRSTEDTSWANLFSSAIAGSLPPVILFFFLQKYFVRGIS
                     GTGLKG"
     gene            13732..14487
                     /locus_tag="PKM49_03550"
     CDS             13732..14487
                     /locus_tag="PKM49_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HNY81919.1"
                     /translation="MRQTKNHLQLSVDKLPETVLLPGDPGRIAEIAACLDHVRQVGDH
                     RGFLTIVGEYKGTEIAACSSGIGGPSVEIAVVELKRYGVRRVIRVGTSGGIGQDVLPG
                     HLVVLSGCVRYSGAADLFVPVNYPAVSDFRTALLLEENAKKTGVPVHFGIGLSLDSFY
                     ATKTHLIQERFPSTIEENLDFWKKAGVLQSEMEAATLFLLSSLLQIRAGAICTVASNL
                     CQKVYPDTPPSNEKAIVAACETAYQLMNTNGRE"
     gene            14493..15254
                     /locus_tag="PKM49_03555"
     CDS             14493..15254
                     /locus_tag="PKM49_03555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY81920.1"
                     /translation="MGNGRLTGKNAIIFGGGGRIGSATARCFFEHGAHVLLIDRDEEK
                     VRALAKTLGNPSRCRYWAQSIENDRDAERLSDWIDTQGVVPDILVNCPAYISRAPFLS
                     SSIEEFDKQMHTNVRLVLIVSRVIAQKMAKAKKGKIINLASLGGVRPEKAHLGHCAAK
                     AAVIAASKVMALELAEFNIQVNVVAPGPTETIPFSSPFYAAHPEILKRIEETTPLGRI
                     GHPEDHTGLLLFLASEESDWITGQVILSDGGLALA"
     gene            15490..>15954
                     /locus_tag="PKM49_03560"
     CDS             15490..>15954
                     /locus_tag="PKM49_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY81921.1"
                     /translation="MKKALLILVLVGLIALTGFSATKIQFWHAMGGWRIDMLNEMAQD
                     FMKLNPDIVVDVQYTGSYDETLNKLVAGIQAGTAPHVIQNFEIATQKMIDGEIAVVMQ
                     DLIDKDPTFDVGKFLPQVLNYYRVNGKLYCMPFNSSNPVMFYNKTLFEKAGLD"
BASE COUNT         3864 a         3896 c         4077 g         4117 t
ORIGIN      
        1 ttccgttttt ttgctaagga atcaattatg ttcacatatt gatttaactt ttctttttca
       61 tccgccttga ttagagatat ttctccagtc tcggaaaaaa ggttagttct aaagacataa
      121 gggcttttca gttgcttttg agtgttgtcc agaatttgag agaaatctgc gagcctctta
      181 tggaggtagt cggcgaactc acgatcttca tagttgatga cgtattgacc atactctgac
      241 aggtcgatct gatttgtggc agcatacaca accccgaaca gcgtaaaaaa gaggattgaa
      301 aaaaacatcg tcatcctaat gttcttcata atcattttct ttccctcccg tatgaaattt
      361 ctttgtttcc ttgtatatag cgtgaaatcc gacaaaacgt cccgagtacc tcgtaactgg
      421 cgttggtatt cactttgtga agcaggattt tatattctga ggggctattt acattgccgg
      481 aagatatgta tagaatattg tatcatgaag aacgtaaaag tcaatgctct ttcaacccca
      541 gaagctctat gataggctcc cttttttcgc tcgcctttac aaagaggctt gaacgccgtt
      601 caagcctctt tcaactccct attttccacg ccgcttctcc ccctgtggct taccacgtct
      661 ccttcgagga tgagctgccc tctgttcgag cggttctctc cgatggcttc ttataggacc
      721 attttttgaa acggggagcg ttttatatgg ccttctacca gcgcgcatac gggtttctca
      781 accgctttct tataggctcc aaaaaagtgt tattctggaa aattgaattc acttctccaa
      841 tcgaaggtcg cgttgtcttc gacataagcc ggccagtgcg cgcaccacag gggcattttc
      901 acatcccgaa cccggtctat ggagggttca tagggcttga tatcgaggat cggcgtgccg
      961 ttctccgcgt cgagacctgc tacttgtagt ctgttttgcg ccctgtcgag cttgacgagt
     1021 acgcaggacg tgatccctat aggattaggc ctgacgggtg atcgcgtcgc gaacaccccg
     1081 attttttcag gccctttcgt atagggtttg tccagaacgt agtatttccg cgtgtcttca
     1141 ttgtcgtaca aatgaaacca ccagacgatc tggatgtggc tgtactcatc gagacctaat
     1201 gtggcgggaa tggcttcttt tttcaatacg atgaagcagt tcccgtcttc gaatttcact
     1261 tctcctatag ggttgaatgt gacggacatc tccttttacc tccttaaagt gcattctaat
     1321 ttggtaggaa cgtatctcac acacatgata caaagaaatc gcgctgctgt cttgacgatt
     1381 tttgccgcga ttccgtgtgc gattttgata caataaaagc ggtttgacga attttgctca
     1441 aggggtagat tctatgaaaa cggaagtgac tcggttaagc tctatattgc ttggtggtgt
     1501 gagcttctac ggtgatccta tcagcgcgaa gggcggatgg gacaccgaaa acgagatagg
     1561 caagacgtgg caaaggttta tgacgttcct tcgtgaaaac cctgaacgtc catattcttg
     1621 cgggaaacag cttttctatg aggttcatct atacggttcg gaaacgaaaa aaaagggata
     1681 tttcgaggta tttgtcggcg aagaggtgaa taccgcgaca ttaccgatcg cgctatgcgc
     1741 gaagtacatt ccggaatccg attacctgaa aatgactctc tttgggaggg agatcgtagg
     1801 ggattggtgg ctcaaactcg agaccgaaat cttacccgct ttgggctttt cggcgaaatc
     1861 ctcccatctg attcaggtat atgacgacag attcaaagga atggacaacg tcgacgagtc
     1921 cattctggac gttttcattc ccatagagaa aaatgatgaa caaggccgta tttgattcgg
     1981 taaagaaaag catcgcttat atcgaagccc atctgtttca cgatatagcc gttcgtgatg
     2041 tttcggactt cgtctcttat tctcaattct atttttcacg tgagttttct aggtataccc
     2101 gtatctccat ttacgattat attataaaaa gaaaaatatc cgaatcgtac aaacgccttt
     2161 ttaaagagaa aatgaaaata atcgacctcg cctttcagta tgggtttcaa tcgcacgaag
     2221 tgtatacacg agcattcaag aaggtattca aagagaatcc cagcgaggcg gtgatctaca
     2281 aacccttagc cgtatacgaa aggatcgacg atcgatattt agagttcgtg tgtgagcttc
     2341 aggtggaaga aatagatcag ccttgtgaag aacgtatctt cgaaatcgct aaaccttcgg
     2401 atgcccaaca gacgaatgat agcctcgtgc aactctctca caccaacctg ttcgaaattg
     2461 agaacgtatt ttacggccgt ttaacctcta ttgaccagaa ttgtctgtcc ttcaatctga
     2521 atgggttgaa agcgatcgcc cgtattcacg ataccgatac gcaatgcgcc ctccgttttt
     2581 ttacggacaa ttattatgat gtggccaaga tgaaggccaa ctatatagtc ctgaggcgag
     2641 cgaacggcta cgtggatgtt ctgattccta ctgcgatttc aacgggagat tgatcgtttt
     2701 atacctatag ggcaagccag tggggctacc gataccgtga aaaacacact gtggggcggc
     2761 cctccgagag ccaagggggc ggcgacgcgt tacgcttttg agaaggacat agagcggatc
     2821 gcggggtcgt aaaaaccact taccccgcga ttgacgcacg agcatcgcgg cgtaacgttt
     2881 atgagttctc ggaagctccc ggaaaagcga aaaacatgcc gaacttaaat tcggcgttcc
     2941 cggagcggag tgcgggggtc gaaaaataca ctccttactc ccctttgaag ggtcaaatga
     3001 gctctaatgg cggctccttt gggggctgtt tttcccctca tcctctttcc atttttcttg
     3061 cgcctcgaaa tgatatccac accctcagta tgcgatgtct tattttaggg tataattcag
     3121 gaggaaacgg aagaaaagat cggggttatc ccggaaaggc aggaaatcca ttggatcttc
     3181 gctccttgat actagaaaag gccttgatcg ttccagcgat actcatcggg ttcacggtgc
     3241 atgagtacgc gcatgccaaa gtggccaatc tccttggaga cgatacgcca tactatcaag
     3301 gccgcttgac cctcaacccg ctggcgcata tcgactggat cggttttttg gccctcttgt
     3361 tggtcgggtt cggttgggca aaaccggtgc aagtgaatcc aaggtctttt aaaaacgtcc
     3421 ggacggattt cttaatggtc accgttgccg ggccgctttc gaacctcgtt accgccttcg
     3481 ccttcgccct cctcaccgct tttcttgcga gcttcatccg ctacgcgcca aacggcaccg
     3541 atcttctttt cacgttgctg aagatcatca ttcacgtcaa ttgtatactt tgcgttttta
     3601 accttcttcc catccctcct ttggatgggt accagatcct aaaaaccctc gtacccgccc
     3661 agtatgggca gtttttttac aatctcgagc gttaccaatt cttcttttta ttctgcctcg
     3721 tcctgattcc gggagcgagt atcatcatca cggttcccgc gcgaagtctc tatttcctgt
     3781 tgaccggttg gataagattc tgattctgtc tacgcgtgtt tttaaagtga ttttgggtgt
     3841 gagtcaagct agggagtgac ataacttgac ccacaccggg ccgtcttctt ctgttccgat
     3901 cgtgctataa tcctgtggag cacaaccccg tggaaaggat ttggtcggac ttgatgcgcg
     3961 aataccttgc ggcatccgaa gattctcgag aggtacaagc tcgaaactgc ggcggatagc
     4021 ctgtcaaata gaacgcgatt gcttacagag ccgtatattt tataaggggg gttactgatg
     4081 cgcgcgaccc tcatcatcgg agacagccgg gctatgaacg aagtgaagaa tggcgcggta
     4141 gacttgatcg tcacctctcc tccctactgg caaataaaag actatggaac ggtagggcag
     4201 accggataca accagactct ccacgagtac tttcgggacc tctattgcgt gtgggccgag
     4261 tgttaccgag tgctgaaacc ggggaggcgg ttgtgcataa atataggcga ccaatttgcg
     4321 cgttctattg tctatgggag gtacaagatc atccccctac actcggaatg catcgcgcag
     4381 tgcgaaagaa tcgggttcga ttatatggga agcatcatct ggcagaagaa aacgacgatg
     4441 aaccccacag ggggcgcgac ggttatggga tcctttccct atcccccaaa cggtatgatc
     4501 gaaatagact tcgaattcat cctgattttt aagaaacctg ggaagggtgt gaaaacgccc
     4561 gccgaaatca aggaaaaatc taagctttct aaagaagaat ggaaaaaata cttttccggt
     4621 cactggtctt tcgggggcgc caagcagttg gagcatcaag cgatgttccc ggaagagttg
     4681 cctaaacggt tgatcaagat gtattctttc tatggagaaa ccgttctaga cccttttttg
     4741 ggtagcggaa ccacgatgaa ggccgcgttg acattgaatc ggcacgcgat cggttacgag
     4801 atcaacgaaa gcttccttcc tgtcatccga gagaaggccg gattttctgg tgagcgcctt
     4861 atgcctgcac atacgctgac aacaatccgg caaagcatgc cgaaggaaga caccagcgct
     4921 gaaatgacct atacccccgg cattcaagac gcgaaacgca aattcggctc caaagggctt
     4981 gacttgaaca aagggagaga gtatttccga gtcgtatcgc tagaagggcc ggataccttg
     5041 gtcctagaaa acgggttgaa ggttcggtta ttaggagtga aaacctttcc agaaaagaga
     5101 gaagcgctcg gtcagtactt gtgtaaatac gtttgtaaaa aaaagatcta tttgcaatat
     5161 gatcaaaaaa ctcccgattc gaactcggaa ggcatcgtga gcgcctacgt gtacttatcg
     5221 aaccgactct gtttaaacct gtccgtgctg aaagccaaac tcgttcacgt ggatccgtcg
     5281 gtcgaccacc cccacaaaga gcgatttagg cgattggctg aaaacgcaag aacgaaagac
     5341 gggtaaagaa cgcctaacgg gggctttgaa acgactcttt attgtcagtt gagaatcaat
     5401 cggatgatat agagcagcca tttcgggaaa tcgggggcca ccttcaagat ctgtctgcgg
     5461 atctccgtat accctctact cacgtgaatc aaggtctccc ctgttcccga ttggctataa
     5521 acggccacga gccctttcgc gatatcaccc acggcgatct tcccgtgagc gacaccgccg
     5581 accgcgaact ctttcgccac acagaagcct ccgattgcgg tttgataaga gaacgcgagc
     5641 ccgccgatcg caagtaagcc ggagattgct atcgaaccga aggacaagaa agcggacaag
     5701 gagagtcctc caaaagaaaa taaaacggaa agggcaatgc caccgaatcc gatcacaccg
     5761 acggatatcc cccctatggc gatcacgcca attgcgattg gcccgatagc gataatgcct
     5821 ttggccactc cgtttggcgt ttttgtgatg tgaacgaggg gaatagagcc gacttgcacg
     5881 ctagagacgt actcgttcgt tagtggaacg agaccgctgt cgatcttttc ttgccaggcc
     5941 ttggcagtat cgatttctcg tcccaagatc aactcgggat catcgatcgt atacaccttg
     6001 tattgttgcg acagttccag aatgatctct cgacgaatct gttgcttcct ctgctcggaa
     6061 gtgtttaaga gcctaaccac attggaagcg taacgtctga atcgttcatt tcgatgcatt
     6121 tgtttacccc catttcaaaa aataggtgaa gcctaatagg cttcctgata aaggtcttcg
     6181 gcatactcta tgaaaaccgt ccgccgctcc gtggtgttga catccgtagc ggcggagcgt
     6241 ttccgcattc gatggtatga ggtggtttca tccctggatc gcggcctgaa gtttatccca
     6301 agatgtgggg atatgagtct gaaacgcgcc gttataatag atacccagcg tcccaaacgg
     6361 gctcccgagc tctttcgcct ccgccgcgct ctctagtttt cggagggtaa cggaaaaacc
     6421 cttctcacgc gcccgttgcg ccgtgagcgt cgcgtattcc tccataaaag ggcactgatt
     6481 cgtatacacc agcgcgaggc catcctgaat gggaatggtc ccgtttttag ccgagggtga
     6541 aaacgcgggc aggggcccat tatttagttt caaagccatc agctcaaaat agggggtcgc
     6601 tttgtcgagg gttacgaatc cctttttttg gaagaacgtt ttatctgtca ggaatggttt
     6661 cttctttccg gaagtgacga ccgctatccc cgccttcccc tgagacttcg cgtcttggat
     6721 gcacaaatcg agcagccggt ttgcccatcc ttgccccttg aacttccccg caacctataa
     6781 acagttgatt atcaggtaat tcgcaccgat caagggcttc cacgccttct ctatcggaag
     6841 gtattcgatg aagaccttcc cgcggtcgtc cagtcgccgg aagaccagcc cgtctcggaa
     6901 attctttttc atccacgctt ttttcgtaaa agcgcgattt tcgttctctt tatcctttcc
     6961 tatcgcgcag caaatgtgct ctttatcgat gttctgctcg gtgatttcga taatttccat
     7021 atttctccct cgatggtctc ctcatgcccg gttcaatgcc gtcggagttt gttggtcctt
     7081 ttgtttaggt agaatagggt cgtgttgact taagataagt tccaaagccg agttttcaca
     7141 agccgtttat agtgtatcac aaaaactatc cgactagaaa gcctcgtatg tgacaagcta
     7201 tggggtttaa tcaagaaaca aggccagaat cgatcgcacg attttagtcc gtgtagtcat
     7261 cgcggcgtaa gcgccccttt ttttcgcgac ggcgcgatac cccggttttc gttcgttcta
     7321 aagataaaaa acagggttcg cacagagggc gcagagaaaa gaatcttgtg aacgtcttgg
     7381 gaacgccaaa ctttagtttg gcatggccct atttttccac tgggagcatt cctgacgcaa
     7441 catcgcggcg taagcgcccc ttcttttcgc gacggcgcga tacgcttttg agtaaggcat
     7501 agagcgtatc gcgagacttc gtgggcctcc gaagtcagcc cgctggttac cccgagttaa
     7561 tctttgcact cttctcgagg tcgaaaaaac actagttaat cccctcgaag ggtcaaatga
     7621 aatttaatgg cggctctttc gcggccattt tgtttctcct cctcctcttt cttttttttt
     7681 gcacctcgat aatataaccg cacggtctcg tttgaaacct tggatttatg ctacaattag
     7741 gactaaccga gagtcgttga cgattttctt accgcgacgc gacagtagac cttgaaggag
     7801 ggagtcaatt atggaaaaaa gagcccgcac aaaggccatt cgccgcaagt tcttcacgat
     7861 cttcatgctg gtgttgtttc tacattcggt gatctggagc gtttctcttt cggatattct
     7921 caacaaagag ccattcacag ggttacccga agtgatttct gagtcgggtc ttccgaacgt
     7981 cgatcctaac acgtactcgg gcgtggtgtt gaaggtgcta cgcttaagaa cgcagcagtg
     8041 gttgggcgct gattgggaac acacggtttt actcgcttac aatgaagagc tctcgcccac
     8101 gatggacacg gccttagtca tgatcggtgg agactataaa tacgatagag aggtgcttca
     8161 agcggtcatc tccgtactcc aggaaagcga cgggttgttc gtgtttctgt tcgatgtgcc
     8221 gaatcaaccg ttattagggg gattgaggga agattggctg atttccgaga ctttcgaacg
     8281 gtattttcaa acgcttgatc caacagtacc cgctttggta ccgatggttc gatcggtgag
     8341 aaaggtgatg gattgggtcg agaatgcctt cggcatccgg aagttcgtcc tttccggagc
     8401 atctaaacgc ggatggacca cgttcttgac ggccgcggtc gatgagcgcg tgaaggggat
     8461 tgttccgatt tcttttgatt ttctcaattt tcccgaacaa ctccgaaagc aaaaagagtg
     8521 gtatggcgct tatagtcctg aaatcgcgcc ttacacggaa aaggacttcc cggcaaagct
     8581 cgaggaagac gttggcaaag agttgctaaa gatcgtggac ccgtttcagt atatcccgac
     8641 gttgacgact cccaagatct gcgtggtggg tacaaacgat ccttattggc acttgttcag
     8701 ctggcaacac tattttccgg agctgtcggg atacaagcag atgctgtaca tcccgaacaa
     8761 gggtcacgga gtagaactct tacggccgat tctggcgatt ctcgcgttga tcgatcacgt
     8821 gtgttgtggt acccagttgc ccgaatacca atggaaggta gacgatcgac agatcgtcgt
     8881 cgagtgggcg tcacaacaag actacacggt tcttgccgct tacctttgga cggcccgttc
     8941 ttccacgatg gattttcgca gggcgaaatg ggcaatctct ccgttaccga ttcctgaaaa
     9001 ccaggctttg gcgcaaatcg cagaattcga actcgagaac gtaagcgcct tcgtcgaact
     9061 acttttcgaa actcacgagg caggaaaggc ggcaaaaaga aggttgtacc tttccacgcc
     9121 tgtatttctg tttcctaagg acagcgaaag aagtggcgga atagagatcc cacggccata
     9181 gacaccctcc ggttttgagc tgaaactgtg atcattttat acctcagtgg tgtgtagcca
     9241 gatcacgtct agaatgttga agctcagtgt gggtataata tcgaggcaca acaaaaaaat
     9301 aagaggagga ggtccgcctt ttgttctttt acgccgcgat gcaagttatc tctgtgcgcg
     9361 atccagcttt tttctctcta aaaatgacat gccaacttaa aagtaggcgt tcccaggacg
     9421 gatcgcgaga cttcgtggac ttccgaagtc agcccgctgg ttaccccgcg ataacccgcg
     9481 aagtttggcg ttcccagaga gacaggccga ctggaagtcg gccccacgaa gagaaggggc
     9541 gcatacgccg caatgactac gtggactaaa aaccgtgcga ttgattctgg ccttgtttct
     9601 tggtcacacc tcataacttg acacacactg aagcgaccct ttaacgcttc agggtttgtg
     9661 ggagttgggg aatttccttt ttcaaactgg cctcggttga ctcccgatag taaatacggt
     9721 ataatataaa tgtcagacgt ccgacgaccg atgggaaaag aggggaatct caattggaag
     9781 cgataaaaaa aatcgaggga aagcgtggca cggttatcga cgaagcgtgg atggagctca
     9841 gagagatgat cctttcgggg atgctcgcgc aaggagaaag gctgccctct gagaccgatc
     9901 tggcccacga gatgggtatc agccgaaact ctttgagaga agccataggt atgctcgaac
     9961 gcgaaggttt gcttctcaaa aaacaaggtg tgggtacctt cgtcaccagc gtgacgccga
    10021 tcatccgcgg agggattgaa agactgaccg gcattatgga ttttgtgaaa gccaacgggt
    10081 atgaagcgca aagccgtttg atatccttta gcgcctccgt tgcaaatccc acggtctcaa
    10141 ctcttctaaa cctgtctccg ggccaggtcg tttacaacct gacgacgatc aaattggctt
    10201 cagaggttcc cgtggcgctg tgtatcgatt atatcccgga atcgatcttt aagacgccgg
    10261 tgaagcccga acgccttcag tattcgctct ttgaggggtt ggagaaagaa tacggcatcg
    10321 aaatctttta cgcggaagcc gagatcatcc cggttatttc ggatcgggac atcgcctcca
    10381 aattacagat tcccgaaggc gaagccgttc tcttgctttc acagattcac ttcggaaaaa
    10441 acaacagaaa aatcctttac tccaaaagtt acttcccaac gggtaagttt acctttagat
    10501 tgatcagaaa acgataggga gcggtagggt ccgaaaatca accgaatcat aggaggtgta
    10561 gctgttgagg aaagcgtttt tgttggggtt gttcttgttg gcaaccttat tgtcggccgc
    10621 gactctcgag gtgctatgga tgggatggcc gaaagaccag gtgatgccgc tgatcgagga
    10681 gttccagaag cagaatccgg gggtagagat cgacattcag ctcgttcctt tcgggcagtt
    10741 gtttcagacg atagaagtcc gattgtcatc cggcggcggg acaccggatg tgtacatcgt
    10801 agatggtccc aataccgcct cgtatgccgc aagaggttat ctccttccgc tagacgactt
    10861 tttttccacg caagagaagg cggcgtggtt cccttcttct atagtcgccg gcagttatcg
    10921 cggtaaattc tattccgtgc catacggcac gtcctcggcc ggactattct tcaacaaaga
    10981 gatctttgaa aagtacgggg ttcccctgcc cgaagagagt ctcgacgcta gaatgacctg
    11041 ggatgaagtc gccgagttgg ccaaacgatt gaccatcgat accgataacg atggcagacc
    11101 agatatctgg gggattgtga tcgagcaaat cgataggccc tatctcttgt ttccactcgc
    11161 ccaatcctta ggcgctcagg tgttgtctcc ggacggactg accgccaccg ggtatatcac
    11221 gtccgagaag ttcgtcgagg ccgcgacctt ctattggaaa ctgtttaacg agtggaaggt
    11281 ggccccccag ggtgtcaatg actccgcgat tgcgcgggag tatttcggta cgggcaaagc
    11341 ggcgatgttg ttaggcaacg aatggaatat cgtcagattg ctcaaatatc ccgaaatccg
    11401 attcgggttg tcgccattcc cgatctttcc gggaggaacg gcggtgacgc caaccgggag
    11461 ctggcacgtt gggatcaact ccaaaacgaa gaagcaagag ctcgcgttga agttcgtcaa
    11521 atacataaca ggaaaagaag cggtcattca atggtatctc gcgaacggcg tcgccccggc
    11581 gagaccggat gtctacgaag cccttccaga gatatttatg cacccgatgt ggcagatgtt
    11641 cttgcaggag atgaaaacga cggcggttac ccgtccattg accccaggtt actcacaata
    11701 tgaattgatc ttgagggaag ccttcaactc catacattac ggtgcgcccc caaagcaagc
    11761 gctcgaagac gcggcgaaaa aaatcgatcg agaactcagt aagtataaat agacgcggaa
    11821 gacctcgtag tcttccttga ataaaacggg gaatatatta ccagaaaggg cgagagtacg
    11881 tcgccctttc ttatcaggga agattccttc tatttcgcca aggcctgagg tcgaacagtg
    11941 ggagttcgtc aggccgatca cccgataggg gacccggggg caagcgatgt ttcgaagcaa
    12001 tagagccgga tggttcttca ttcttcctgc tttcgtgttt ttgggacttt tcaaaatcgt
    12061 tccgataggg gtttcggtcc tagaaagcct gaggcagacg acctacaccg gtcggtcata
    12121 tttcgtcgga ttcgaaaact atacctacct gtttttcgaa gacccgacct tctggaattc
    12181 gttctggatc accctgatct attcgctcgt cgtcaacccg ctgatcattc ttatcgcgct
    12241 ggtgatggcg ttgctcctga atgaaaacca ctttttcacc aagtttttta ggacggtatt
    12301 ttttttgccg accgctatct catttgcggt cgtgtcggtg atctggtcgg ttgtgctcga
    12361 cccgcattat ggtctggcga acggtttttt aacgttgttc gggcttccgg ctcagccgtt
    12421 tttcgcgtcc accgctcagg ccttatggtg tctggtgttc ctgaacgtgt ggaggagcgc
    12481 ggggtattgg atgatgtact ttttagccgg actccagaat attccggaaa gcctttatca
    12541 agcggccgat atcgatggcg cctccgcttg gacgaaaact cggaaaatca cgttgccttt
    12601 gttgtctcgg accttcgcct tcgtcctggt ggccaacacc gcgttcaatt ttctcacctt
    12661 tgcccctgtg tatatcatca cgaaaggagg gccccgcggt tcgaccaacc ttctgatgta
    12721 tgaatcctat aagagcgcct tcgtcaacct ggatatgggg cgagccagcg cgatcaccac
    12781 gatactcttg gggatcatcc tgatcttcag cctcttcgaa tttaagtgga caaaggccgg
    12841 tttcgaatac taaggggatc gtgatgatga gacggtataa aaaacccctc gttttagccg
    12901 cggaatttgt cttgttgacc gcgatcagtc tgtttttcat cctgccgata ctctggatcg
    12961 tcgcctcagc gctgcgccct tcgggaccct tgtacaccta tgcgaacccg ttgactctgc
    13021 agacgttttt tcccaccacg gtcaccttcg acaatttcgt acacatcttc aaaaacctgc
    13081 acttcgggag agccttgctc aacagcctcc tggtggctgt cgcaactatc gcgataagtg
    13141 tcctcatctg ttctatggcc ggttttggac tggcgaagtt tgcctttcga gggaagaacc
    13201 tcttcttttt catcgttttg atcactttta tggtcccctt cgaatcgatc gtgattccgc
    13261 tcttcgtttt agtcaagcga ctgaacatcg acaacacgta cttcgcgctc atcttgcctg
    13321 ccgcggggaa cgggttagcg atctttcttt tccgccagtt tttttctgag gtcccgacgg
    13381 agatcctaga agccgcccga atcgatggcg cctcgtggat caggatcttt cggaagatca
    13441 tccttccatt gagcatccca gcgatcgtga ccgtgatcgt tatggtcttt atgttccaat
    13501 ggaattctct gttttggccg ttgatcgcga cgcattcgag ccggttcgag gtggtgcagg
    13561 tggcgatcac agcgcaccgc tctaccgaag acaccagctg ggcgaaccta ttcagctcgg
    13621 cgatcgcggg aagcctgcct ccggtgattc tgttcttttt tttgcagaag tacttcgtcc
    13681 gcggtatatc aggcaccgga ctgaagggat agaacaagga gagtggaacc gatgcgtcaa
    13741 acgaaaaacc atttgcagct cagtgtcgat aagctgccag agactgtcct attgccaggc
    13801 gacccgggga ggattgcaga gatcgccgcc tgtttggatc acgtgcgcca ggtgggggac
    13861 catcgcggct ttctcacgat cgtcggagag tataagggaa cggagatcgc tgcttgctcg
    13921 agtgggatag gcggcccgtc cgtcgaaata gcggttgtgg agttgaaaag atacggagtc
    13981 cgccgggtta ttcgggtcgg tacttcaggc gggatcggac aggacgttct gccggggcat
    14041 ctggtcgtat taagcggatg cgttcggtat tccggggctg cggacctgtt cgttccggtc
    14101 aattaccccg cggtttcgga cttccgaacg gcgttgttgc tggaagagaa cgccaaaaaa
    14161 accggagttc cggttcattt cggcatcggc ctttcgctcg actctttcta cgcgacgaaa
    14221 acccacctca tccaagagag atttccttcg acgatcgaag aaaatctgga tttttggaaa
    14281 aaggcgggtg tccttcagtc ggagatggaa gcggctacgc tgttcctcct gtcttcgttg
    14341 ctccagatac gagctggagc gatctgcacg gtggcttcga acctatgcca gaaagtctac
    14401 cctgatactc ctccgtcgaa cgaaaaggcc atcgttgccg cgtgcgaaac agcctatcag
    14461 ttgatgaaca cgaatggaag ggagtgaagg tgatggggaa cggacggtta acgggaaaga
    14521 acgcgatcat tttcgggggt gggggaagaa taggcagtgc gacagctcgg tgttttttcg
    14581 aacacggagc acacgttctt ttgatcgacc gggacgagga gaaggtacgc gctttagcca
    14641 aaactctggg gaatcccagt cgatgccggt attgggcgca atccatcgaa aacgatagag
    14701 acgcggaacg gctatcggat tggatcgata cgcagggggt cgtacccgat atattggtca
    14761 actgtcccgc ctatatatcg agagcgccgt tcttatcctc gtcgatcgaa gagttcgata
    14821 aacaaatgca tacgaatgtg cggctggtct tgatcgtttc gagagtgatc gcacagaaga
    14881 tggcgaaagc gaaaaagggg aagatcatta acctggcttc gctcgggggg gtccgcccgg
    14941 aaaaggcgca tctgggacat tgtgccgcaa aagcggcggt catcgcggcc tcaaaggtta
    15001 tggctttaga acttgccgag ttcaatattc aggtcaacgt cgtggccccg ggcccgacgg
    15061 agacgatccc tttctcctct cccttttatg ccgcccatcc ggagatcctc aaacggatcg
    15121 aagagactac cccactaggc agaatagggc accccgaaga tcacaccggc ttgcttctct
    15181 ttctggcctc cgaggaatct gattggatca ccgggcaagt cattctttcc gacgggggtt
    15241 tagcgttggc ttgaaggaaa cccagatgct gggactttct ttgtcgacga aacgctcggc
    15301 gctcagctta taatcggccg atagtgtttg gcacttcctc tatttatgga ttaggcgccg
    15361 caattgagtt ggaaaaccag cccataagcg gttttagtaa acgtccttgc aaagtgataa
    15421 gacttaggtt ataattagaa gcacgcgcct gtgctttttg cctgaaaaaa attctaagga
    15481 gggatttaag tgaagaaagc gttgttgatt ctggtactgg tcggtttaat cgcgttaaca
    15541 gggttttcag cgacgaagat tcagttctgg catgcgatgg gaggctggcg catcgatatg
    15601 ctgaacgaga tggcccagga ctttatgaaa ctgaatccgg acatcgtggt ggatgttcag
    15661 tataccggga gttacgacga aaccttgaac aagctggtag ccggaatcca agcgggaacc
    15721 gcgcctcacg tcatccaaaa tttcgagatt gctacccaga agatgataga cggagaaatc
    15781 gcggttgtga tgcaagatct catcgataaa gaccccacct tcgacgtggg gaagttcttg
    15841 ccgcaagtcc tgaactatta tcgagtcaac gggaaattgt attgtatgcc gttcaactca
    15901 tccaaccccg taatgttcta taacaagact ttattcgaaa aagcgggact agac
//
LOCUS       DAOPXN010000039        15750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3798_length_15750_cov_5.426569, whole genome shotgun sequence.
ACCESSION   DAOPXN010000039 DAOPXN010000000
VERSION     DAOPXN010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15750
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3798_length_15750_cov_5.426569"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1436
                     /locus_tag="PKM49_03565"
     CDS             249..1436
                     /locus_tag="PKM49_03565"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNY81922.1"
                     /translation="MNPKWLKALAYGFVGYRILTVEFQPGGSAALALVLLLVFISVSQ
                     WRSNYLTARPFLFWVTAVAEVAFCVYLFHRYGGFLSIYLFSALMDTTSAFRHHRRVLP
                     SLAAVGLFFAVYYRFFPSQEAFTLSLAIADLVLLLFFAGFLLFLQEEKSRKVVAQELY
                     DQLRKSEDRLKDAIGALEQYSSTIEELTLLRERARISRELHDSVGHALSTLSLQLRAI
                     QTIVEKDAPKAKSMLDELGQFAHNALENVRRAVRELRPIEFEAFEGIFAIEELARNFS
                     KLTGIDTRLLLSQNRYPMNSDQSLQLYQIVKESLSNSMRHGKARHITIALQFLEDRIY
                     SQIKDDGEGCAQIVFGMGLKGIRERTMSLGGEMRAFSTPGKGFELVVELPKRPSMPAS
                     KVE"
     gene            1438..2082
                     /locus_tag="PKM49_03570"
     CDS             1438..2082
                     /locus_tag="PKM49_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY81923.1"
                     /translation="MIRLLIVDDEKLICEGLRVLMGVYEDIQVVGTCGNGLEALRFCR
                     QNPVDVVLMDIRMEKCDGVEGTRLIKEANPAIKVVILTTFQDEEYIYSALSLGASGYL
                     LKDSSSDRIYEAIRSAHFGNVAVHPEIVKRMVSAKETPLAKDKIKKAYQLTDKELELI
                     GFVCQGLSNKEIGEKLFLTEGTVKNNIGKILSKLNLRDRTQMIIFAFQNHLVES"
     gene            2213..3151
                     /locus_tag="PKM49_03575"
     CDS             2213..3151
                     /locus_tag="PKM49_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81924.1"
                     /translation="MYEISIQNVTKRYDDQLALDNVSLRIEEGEIFGLLGPNGAGKTT
                     LIHLLCGLIGMDSGDILIGGRSMKKSPEKLKALIGYVPQEIVLFDTLTVKDNLEYFGR
                     LYHLSGTRLKEKIQSALAITGLKDHHKQKVKKMSGGMKRRLNIACAILHDPKILVLDE
                     PTVGVDPQSRNHILEAIREMNRTQKMTVLYTSHYMEEAEALCDRLMILDLGKELVQGT
                     KTEIIRTVTNESVVEILTDYLPTGITEAFQEVPGVKKASVRDGKIHLIVDSSDPIVEK
                     AVRLLDGLEVKVRNLSVDNPTLETVFLSITGKALRD"
     gene            3163..4299
                     /locus_tag="PKM49_03580"
     CDS             3163..4299
                     /locus_tag="PKM49_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81925.1"
                     /translation="MFDSVWKATGAIFSKDFKVIKSQGFSTLLLFLFLLALGLLYGLM
                     YQNVFDQNIHWTPLPVFIFDETGSSEPGFAEDLLSPDRFPFIELHKAFSSLDFEEGLA
                     NQKNAVGLLIKEEEGQTKIYWRSVGEKSIEKNILFQAIEKGVEEALWHTRTYAILGPR
                     AADFMTEWEILAARPLIREVPREDIRVLSSLEYYLISLFTGFCILFTTEFVRDREQRL
                     IARSFTAGIRKSTLYWSNFLSNFVLVFAVALVYFTIVFGIFLRIPVPLGPFFGAVIVQ
                     AMVVSAFQSLLGGVCKNRKVELVISLLALFTMLYLGGAFFPVEVMALERIAEFMPNFQ
                     LFKLYEGLVLGHPGLDATRLGILFAQSAVMALVGWFVFKKSEVL"
     gene            4301..5413
                     /locus_tag="PKM49_03585"
     CDS             4301..5413
                     /locus_tag="PKM49_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY81926.1"
                     /translation="MRLHVLYGNFRRVIKDPILILTIFSLPLIGILILSLLVHGGEVI
                     QQVPVGVIDLERTPLSEHLIDRLKTDKNYEVSLYAADQGQTLFENRRLGALITIPEGF
                     TASFRTETPKEILFSVLPTIPSGMIEASLQDGLRQAVRDDWLRGDTPRPDLDSAVSDS
                     PIALVTPEPEKGSTNTIVLNIIIFIMMFSATFIASDLLQLRQKNLLFRFYATPNSPTG
                     IALSILGSMLALVALHTLLLFIVGSVFSPAPLIQNDLLGGVMLLGSFLLVTLSLGVLI
                     GRICRRPSMISIVSNLIIIPTGVISGTFVPKEFLPEAVYKLAVLAPQYWVINGIERLN
                     RAEGFLSVVPNTLILLLFAFCLFSAGIFRFREMVKP"
     gene            6197..7441
                     /locus_tag="PKM49_03590"
     CDS             6197..7441
                     /locus_tag="PKM49_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81927.1"
                     /translation="MKKVWITLLVTVLVFSAIGMAKTKITYFGDNDPNSAEAILTRLF
                     NSSQDEIEVEFLLQSWSTDDKHTALVTRFGAKDPNPTVYLGDVIWPAEFAAAGWALDL
                     TPYFPKSEQDKFLPGTIKSATYLGKIYAIPSFTDGGLLYYRTDLLEKYGYKPPTTWEE
                     LKTIAATIAKKEGIDGFVFQGAQYEGLVCDAVEFIAANGGEVIDGNGNVKVNSPEVIQ
                     ALEFMRELITSGAAPDKVFTFMEEDARMAFQQGNAVFMRNWPYCHSPSNMAGDQSAVK
                     GKYGMIPMPKGPKGSGGAATLGGWMLFVNPFASKAEQAAGVQFAKFITSESAQIIRNL
                     VAGNAPTRIAVYSNPTVLRANAIVETLYPIMINAVPRPVTPFYAQVSNALQEEFHAVL
                     TGKKTAADAVKALERKIKEIYE"
     gene            7505..8458
                     /locus_tag="PKM49_03595"
     CDS             7505..8458
                     /locus_tag="PKM49_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY81928.1"
                     /translation="MRKRYSREKQETRMGLLFLLPSLIVIVLITFLPIGQTFYRSLFR
                     WDIKFESRQYFTGLTNYTAIFQGLVNPDEALSWTVSFWHSAWVTFLFTVVAVSIETVL
                     GLLIALIINREFRGRGLVRTATLVPWAIPTVASAQMWRMMFSSQGGVINDIAKKLGLI
                     TDYVNFLGDSTTTAFWSMVTADAWKTTPFMALLLLAGLQTIPLQLYEAARVDGASKWR
                     QFFSITLPMLKPSLLVALIFRTLDTFKAFDIAQVLTGGGPASGTELLSLFNYKLMIQQ
                     GQHGRGSAVAIMLFVFTMLIAVFFIKVLGADPYASKGRELK"
     gene            8455..9285
                     /locus_tag="PKM49_03600"
     CDS             8455..9285
                     /locus_tag="PKM49_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY81929.1"
                     /translation="MKKRWIPRLLLTLAVIVIIVFMFFPFYWLFIMSLKGDLDLYKMP
                     PDWIAKNPRWDNYKTIFTDHNLGQNLQNSFVIAFSTTLISVLIGSWAAYALAKFRMRF
                     KSLILGIVLAVSMFPGIVVVSPLYMQFSDLKIINTYWALILPYLTFSLPMTIWILQSF
                     FKQIPDALTEASVIDGAGPMRTLFQILFPLAAPGIFTAFILTFIGAWNELLFASIFIT
                     DYRKFTVPRALTLFSGQYTSSWQLIAAASVVITLPLIVMVLLLQKWIVAGLTAGGVKD
                     "
     gene            9303..10904
                     /locus_tag="PKM49_03605"
     CDS             9303..10904
                     /locus_tag="PKM49_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY81930.1"
                     /translation="MDKPWWKGAVIYQIYPRSFKDANQDGIGDLKGITEELDYIQELG
                     VDAIWLSPIFKSPMKDFGYDISDYLEIDPIFGTMADFDRLLDAAHERGIKVILDQVYN
                     HTSDRHPWFVESRASRDNPKADWYIWVDGEKGQAPNNWLSYFGGGAWEWEEARQQYYL
                     HLFVKEQPDLNWRHPDVRKQVMDSIRFWLDKGVDGFRFDVVNLYYKDERLRDNPTKYP
                     DEPIAFNRHHHLYTRDRPETEGAIEEIRELMDRYKDRVSIGEVATELGQAQYFAYTRP
                     NRLNLAFNFDFKELRPFAAKTYVEHIKLNEAVFRDFAWPSYVLGNHDSKRFLSKLGEG
                     EDAVRKAKVLAALLLTLRGTPFVYYGEEIGMREAVIPYEKIVDPQGKNLWPNNVGRDG
                     CRTPMPWNASPHAGFSQTEPWLPIGEDAREVNVERQKAEPGSMFHYYKQLIRIRKESE
                     ALRFGRLELLDTKDPALMGFFRIGESERVLVLLNFEDRKVVLNLKGFGVEVRRYIGGT
                     TSRPERFNAASTSLEPYEAAILEVS"
     gene            complement(10933..12264)
                     /locus_tag="PKM49_03610"
     CDS             complement(10933..12264)
                     /locus_tag="PKM49_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81931.1"
                     /translation="MRPRIPQSRSIYSLKRLKPYLAALWVILFLSFASLSFCLGQIED
                     PCAEESGGCSLLDILGEGLDVHTDSTVQIRREPENGRVFLYLFYGDDCRHCQELRAFL
                     NTLVDSDAYEEFEGYTVNRALYDQYQSVDPRLLMNAYEISRDAANASILESFFAAYDT
                     RFTGIPILFVGREAFSGFDRTTMPDEILRAIITAYGVAPVSSDNTITLPLFGKIDRSS
                     PLFFLTILIGLLDGFNPCAFWVLMFLLSLLSTAKSRKRMLLIGSVFVIASGVVYFGFM
                     SAWFNFFSLIGMHRIITWILSVVAIGMGLINLKEVFFFKKGVSLMISEKQKPKLYQRV
                     RKIIQTRSTLLAIALTVALALFVNLIELTCTMGLPALFSKILTERKIAIGAKYFYLVL
                     YNIAYIFPLWLIVVGFSVTLGKYKMTEKSAKVFKAISGLLMIALGIWLWFA"
     gene            complement(12212..12925)
                     /locus_tag="PKM49_03615"
     CDS             complement(12212..12925)
                     /locus_tag="PKM49_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81932.1"
                     /translation="MSRVLFSFIVGLFVCTSLLALQFQDYAVYNEIENGLKVAALTQK
                     GVVLIFTRPSCPSCTQLKAEGLATLELANLLRLNHIVIVAEAEKDFYARFPFDVFLNP
                     DITQYETLSYYDIAAKKFHVSAIPRTFLLDSQFQTVGSVERYLSYESYVTSLRSVMNP
                     AMSQPVRLIRSVTSKEATLLTATLPNVRTVTFSEFAKLFPTYDWMGYYILLNTSVQEV
                     QEFAAANPTVPLNLLVKAP"
     gene            13990..14652
                     /gene="pgmB"
                     /locus_tag="PKM49_03620"
     CDS             13990..14652
                     /gene="pgmB"
                     /locus_tag="PKM49_03620"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HNY81933.1"
                     /translation="MFDIKGYIFDLDGVIVDTARFHFLAWRETARELGFELTERDNER
                     LKGVSRMESLEIILRLAGIHLDEERKWAFTEKKNTRYLEWVDTLRPSDTLPGIADFLR
                     SLKEAHRPVALASASKNARRVISLLEIASLFDAVIDGSMIEKTKPDPEIFIKAAQALG
                     IRASECVVFEDAQNGIEAAKAAEMTAVGVGSPEHLGEADLVIPDFSPSHIYLLKQRGI
                     VR"
     gene            14649..>15750
                     /locus_tag="PKM49_03625"
     CDS             14649..>15750
                     /locus_tag="PKM49_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HNY81934.1"
                     /translation="MNEIVFTEDHWDPSIYPLHETLFAQANGYLGIRGSFEEGFAGPS
                     PKSVRGTYINGFYENEKILYGESAFGFAKTNQIMLHIPDATRLRLTVDGEPVFIDTGR
                     LWAYKRVLSLADGVLTRTMDWESPLGKRVLFRFRRFVSLQDPHLLAMHLTLTPINFSG
                     AIELVSLIDGNVRNIQNEDDPRVGSQSEGLFLEPISSAGYAKEGLAISVFRTRHSNLT
                     LGCSIHHLLSREPAEAPEVTCENRQAAIRYVLLLDPEQEASLTKFACYTTSQETPVEE
                     IDRFLRQKGAHALQEGFDAYAASQGEILSRYWRVAEVKIENQPEVEKAMALNQFHLYQ
                     STGKDGKRNVASKGLTGEGYGGHTFWDTEIYMI"
BASE COUNT         3697 a         4057 c         4254 g         3742 t
ORIGIN      
        1 gcgatgacta cgtggaccaa aaatcgcgct atcgattctg gccttgtttc ttgatggcgc
       61 ctcataactt gacccgcacg cacttgcata cttcccgcct tttatggtac aatcaattgc
      121 tattctcgtc aatctaatgg accccttcga gggtcttttt ttctcttttt ttcctttact
      181 tcagccatta aacccacaca cggaaaaaac gtgctatact tcaatcaaat ccaagtggag
      241 agatcgcatt gaatccgaaa tggctgaaag cgctcgctta tgggtttgtg ggctatcgaa
      301 ttctcaccgt cgaattccaa ccgggtggtt cggcggcgtt ggcgctggtt ttattgctgg
      361 tcttcatctc ggtatcgcaa tggcgaagca actacctcac ggcgcgaccg ttcctgtttt
      421 gggtcacagc tgtggcggaa gtcgcgttct gcgtttacct ctttcaccga tatggcggtt
      481 tcctttcgat ctatctcttt tcggcattga tggacacgac ctccgcgttc cgtcatcacc
      541 gtcgggtcct tcccagcttg gccgcggtgg gcttgttctt cgcggtttat tatcggtttt
      601 ttccatcaca agaggctttc accttgtcgt tggcgattgc agatcttgtc ttgctccttt
      661 tttttgccgg gttcttgctc tttctccagg aagagaagag tcgaaaggtt gtcgcccagg
      721 agttgtacga tcagctgagg aagtccgaag accgcttgaa ggatgcgatt ggtgcgctag
      781 aacaatattc gagcacgatc gaagaattga ccttgctgcg agagcgcgcg cgcatctctc
      841 gcgagctaca cgatagtgtc ggccatgccc tctccaccct gtcactccag ctgagggcga
      901 tccaaaccat cgtggagaag gatgccccga aagcgaagag tatgctggac gaactcgggc
      961 aatttgcaca caacgcgttg gaaaacgttc gacgcgccgt tcgggagctg cgacctatag
     1021 aatttgaggc gttcgaaggg atcttcgcca tcgaagagtt ggcgcgcaac ttcagcaaac
     1081 tcacggggat cgacacccgt cttctcctat cgcaaaaccg ctacccgatg aactccgacc
     1141 aaagcctcca actctatcag atcgtcaaag aaagcctgag caacagtatg cgtcacggga
     1201 aagcccgcca catcaccatc gcgctccaat ttttggaaga ccgcatctat tcccagatca
     1261 aagatgacgg agagggatgc gcccaaatcg ttttcgggat gggactcaag ggaatacgag
     1321 agcgaacgat gtcgctgggt ggagagatgc gcgccttttc gacaccgggg aaagggttcg
     1381 agctcgtggt cgaactaccg aaacggccgt ctatgcccgc gtcaaaggtg gagtgagatg
     1441 attcgacttc tgattgtgga cgatgaaaag ctgatctgcg aggggttacg ggtgctgatg
     1501 ggggtgtatg aggatattca ggtcgtcggg acctgcggga acggtttgga agcgctgcgt
     1561 ttctgccggc agaacccggt ggatgtcgtt ctgatggata tccggatgga aaaatgcgat
     1621 ggggtcgaag ggacccgctt gatcaaagag gcgaatccgg ctatcaaggt ggtcattctg
     1681 acgacattcc aagacgagga gtacatctac tccgccttgt cgctcggagc aagcgggtac
     1741 ttgctgaaag acagttccag cgatcggatc tacgaagcca tacggagtgc gcattttggg
     1801 aacgtggccg tgcatcccga aatcgtgaag cgtatggtca gcgcgaagga aacgccgtta
     1861 gcgaaagata agatcaaaaa agcttatcaa ttgaccgaca aagagctcga gctcatcggg
     1921 ttcgtctgcc aggggctatc caataaagag atcggtgaaa agctgttcct caccgaagga
     1981 acagtgaaga acaacattgg aaagatcctt tccaagctga acctgcgtga ccgcacccag
     2041 atgatcatct tcgccttcca aaaccatctg gtagagtcat agcccaatcg tgacctctgg
     2101 tgatgaccta agtcacttcc atatcccgcg aacgatgccc taggctactt cccattctgt
     2161 gtgaaaccct ctataatgcg gtaaaagatt gacacaaaag gaggttttca cgatgtacga
     2221 gatttctata cagaacgtga ccaagcgata cgacgatcaa ttggctctgg acaacgtgtc
     2281 cttgcgcatc gaggagggcg agattttcgg gttgttgggc ccgaacggcg cggggaagac
     2341 gaccttgatc catctgctat gtggactgat cgggatggac agcggggata tcctgatcgg
     2401 aggtcggtcg atgaaaaaat cgccggaaaa actcaaagcg ttgatcggct acgtccccca
     2461 ggagatcgtc ctcttcgaca cacttaccgt gaaggataat ctggagtact tcgggcggct
     2521 gtaccacctc tccggaaccc gattgaaaga gaagatacaa tctgcgctcg cgatcaccgg
     2581 gttgaaagac caccacaagc agaaggtgaa gaagatgtcc ggcgggatga aacgtcggct
     2641 gaacatcgcg tgcgcgatcc tacacgaccc caagatcctg gtactggacg aaccaacggt
     2701 cggagtggat ccccagtctc ggaaccatat cctcgaagcc atccgagaaa tgaaccgcac
     2761 gcagaaaatg accgttctgt acacttctca ctatatggaa gaggcggaag cgctctgtga
     2821 ccgactgatg atcctcgacc tgggaaaaga actcgtacag ggcaccaaaa ccgagatcat
     2881 cagaacggtg acgaacgaat cggtggtcga aatcttgacg gattaccttc caaccggcat
     2941 cacagaggcc tttcaggagg tgccgggtgt gaaaaaagca tctgtcaggg acggaaaaat
     3001 acatttgatc gttgattcga gcgatcctat cgtcgaaaag gccgttcggc ttctcgatgg
     3061 gttagaggtc aaggtccgca acctttcagt agacaacccc accttagaaa cggtctttct
     3121 gagcatcacc gggaaggcat taagggatta ggggggacgc atatgttcga cagcgtatgg
     3181 aaagcaacgg gcgctatctt ttctaaagac ttcaaagtga ttaaaagcca ggggttttcg
     3241 acgctcctcc tctttctctt tcttttggcg ttaggcttac tatatggatt gatgtatcag
     3301 aacgttttcg atcaaaatat ccactggaca cccttgcccg ttttcatctt cgatgagaca
     3361 ggaagttcag agcccggctt cgccgaagac ctgctgagcc cagatcggtt tccttttatc
     3421 gagcttcata aggccttttc cagtctcgac ttcgaggaag gattggcaaa tcagaaaaat
     3481 gcggtcgggc ttttgatcaa agaggaggaa gggcagacca agatctattg gcgctctgtc
     3541 ggtgagaaga gcatagagaa gaacattctt ttccaggcaa tcgaaaaggg ggtagaagag
     3601 gcgctatggc atacgaggac ctacgcgatt ctcgggcccc gagctgcgga ttttatgacc
     3661 gagtgggaga tcctcgccgc ccgccccctc atacgcgagg ttcctcggga agacatccgg
     3721 gtgctgtcgt cgcttgagta ctatctgatc tcgttgttca cgggcttctg tattctattt
     3781 acgacagagt tcgtccgaga tcgagaacaa aggttgatag cgcgttcgtt taccgcgggt
     3841 ataaggaagt cgacgctcta ctggtcgaat ttcctgtcga acttcgtatt ggtttttgca
     3901 gtagctcttg tgtacttcac gatcgttttc gggatattcc ttcgaattcc tgtcccgttg
     3961 gggccttttt ttggcgcggt tatcgtacag gctatggtcg tatccgcctt ccaatcgttg
     4021 ttgggcggag tgtgtaaaaa tcggaaagtc gaactcgtca ttagcctcct ggccttattc
     4081 acgatgctgt atttaggcgg cgcctttttt ccggtggagg taatggcttt ggaaaggatc
     4141 gcagagttta tgccaaactt tcagctgttc aaactctacg agggccttgt tttaggacat
     4201 cctggcctcg acgcgacgcg tttggggatc cttttcgccc agtcggcggt gatggccctc
     4261 gtcggttggt tcgttttcaa aaaaagcgag gtgttataga atgcgacttc acgtactgta
     4321 tggaaacttc cggcgggtga tcaaggaccc gatactcata ctcacgatat tttcccttcc
     4381 gcttatcgga attctcatcc tgtccctctt ggtgcacggt ggtgaggtca tccagcaggt
     4441 tcctgttggc gtgatcgact tggagagaac ccccctctca gaacacctga tagacaggtt
     4501 aaaaaccgat aagaattacg aagtctcact ctacgcggcg gaccaagggc aaacgctttt
     4561 cgaaaaccgg cggcttgggg cgcttatcac cataccggaa ggcttcaccg caagctttcg
     4621 tacggaaacg cccaaagaga tcctgttcag tgtgctgccg accatcccct ccggaatgat
     4681 cgaggctagc ctgcaagacg gtttacgtca ggcagttcgg gatgactggc tccgaggcga
     4741 tacgccgcgc ccggacctcg actccgcggt ttctgacagc ccgatcgcgt tggtgacgcc
     4801 cgaacccgag aaaggctcga cgaatacgat cgtcctgaac attatcattt tcattatgat
     4861 gttttcggct acctttatcg cgtcggatct cctccaactc cgacaaaaga acctgttgtt
     4921 tcgattttac gcgacaccga actcacccac gggtatcgcg ctttcgattc ttggatcgat
     4981 gctcgctttg gttgcgttgc ataccctcct gttgttcatc gttggatcgg ttttttcacc
     5041 cgccccattg atccagaacg atctcttggg aggggtgatg ctcctcggtt ctttcctgct
     5101 ggtcacgctc agcctcgggg tgttgatcgg ccgaatctgc cgacgtccgt cgatgatttc
     5161 aatcgtttcc aacctgatca tcatccccac aggggtgatc agcggtacgt tcgtcccaaa
     5221 agagttcctg ccggaagcgg tctacaaact ggcggtttta gcgccgcagt attgggttat
     5281 caatggcata gaacgcttga accgcgccga aggattcctc tcagttgtac caaacaccct
     5341 catccttctc ctcttcgcgt tttgcctctt ttccgcaggg atattccgtt ttcgagaaat
     5401 ggtcaaacct taacattcga gccctcgata accgaatcaa aaacgtatcg cgcctcgaga
     5461 aaaaaagggg cgcttacgcc gcgatgctca ggtgagttat cgcggggtaa ccaacgggac
     5521 cccgcgatcc cttcttcgcc cttctaaaga gggtatcgcg ccgtcgcgaa aagaaaggac
     5581 gcttacgccg cgatgcctac aaggttatcg cggggtaagt ggtttttacg accccgcgat
     5641 ccccaccctt tgccattttt tcctctgtgt tctccgtgcc ctctgtgcga accctgtttt
     5701 ttgatcttcg gaaacgaatg aacggggtat cgcgccgtcg cgagaagaag ggacgttcac
     5761 gccgcgatgc tcaggtgggt tatcgcgggg taaccaacgg gaccccgcga ttcgcctttt
     5821 gcccttctca aaaatgtatc gcgacgtcgc gaaaaatcgc gcgattgatt ccaaccttgt
     5881 ttcttgatag caccccataa cttgacaggc acgtcttttt tgtatttctc gagttttggt
     5941 atataattaa gagcgggaaa cgcaaaatgc cattgtgatt ccattcttat ttcttctgtt
     6001 cagtcgtctt ttgtgtgggg tcaacttctc ttatccaaca gccggaaaga ttcggccaga
     6061 agtgtcgagc tgcttagtca tacctgtttt tgtgcgtcac cgtttgaaat gtgccttagg
     6121 cgctcggggc gctatgattt ttagagaaat aatcctttat tcttttagga atcaatccca
     6181 ttttggaggt gtcagtatga agaaagtatg gatcacgtta ctcgtcacgg tcctcgtctt
     6241 ttctgcgata ggtatggcga agaccaaaat tacctacttc ggggacaacg acccgaattc
     6301 ggctgaggcc attttgacaa ggctgttcaa cagctctcaa gatgaaatcg aagtcgaatt
     6361 cctgctgcag tcctggtcta cagacgacaa acacaccgcg ctcgtcaccc gctttggggc
     6421 aaaagacccc aatccgaccg tctacttggg tgacgtcatt tggcccgcag agtttgccgc
     6481 agccggatgg gcgttggacc tgacccctta ttttccgaag tccgaacagg ataagttcct
     6541 gccgggaacg atcaaatcgg cgacctacct tggtaagatt tacgcgattc ccagtttcac
     6601 cgatggcggt ctgctgtact accgcaccga cctgttggaa aaatacggtt ataaaccgcc
     6661 gaccacctgg gaagagctga aaacgatcgc ggcgaccatc gcgaagaagg aaggcatcga
     6721 tggcttcgtc ttccaaggcg cccagtatga aggcttggtc tgtgacgccg tcgagttcat
     6781 cgccgcgaat ggcggggagg tcatcgatgg caacgggaac gtgaaagtga atagccccga
     6841 agtcatccag gcgttggagt tcatgagaga actgatcact tccggcgctg cccccgacaa
     6901 agtgtttacc tttatggaag aagacgcccg tatggccttc cagcaaggga acgcggtctt
     6961 tatgagaaac tggccttact gccactctcc ttccaatatg gctggagacc aatctgcggt
     7021 taaaggaaaa tacggaatga tcccgatgcc gaaaggtccg aaaggatccg gaggcgccgc
     7081 gacgcttggc ggttggatgc tcttcgtgaa cccgttcgct tcgaaggcag aacaggccgc
     7141 tggcgtgcag ttcgcgaagt tcatcacgtc ggaatccgcg cagatcatac ggaatttggt
     7201 ggccggcaac gcgcccaccc gcatcgccgt ctactcgaat cccacggtac tgagagccaa
     7261 cgcgatcgtc gaaactctgt atccgattat gatcaacgcc gttccgcgac cggttactcc
     7321 gttctacgcc caagtcagca acgcgcttca ggaagagttc cacgccgtcc tgaccggcaa
     7381 gaaaaccgcc gccgatgcgg tcaaagcctt agaaagaaag atcaaagaga tctacgagta
     7441 gtctgtacat tacgtttttt ccggcccttg agagggccgg atttttttcg gaggtgtttg
     7501 gaccttgaga aaacgctatt ctagagaaaa acaagaaacg cggatgggct tgctcttctt
     7561 gcttccgtcg ctgatcgtca tcgttctgat taccttcctc ccgatcggcc aaaccttcta
     7621 tcgctccctg ttccgctggg atatcaaatt cgaaagcaga caatacttta cgggactcac
     7681 caactacact gcaatattcc aaggcttggt caatccggac gaagcgctca gctggaccgt
     7741 cagtttctgg cattccgcct gggtcacctt cctgttcacg gtggttgcgg tatcgataga
     7801 aacggtgctg gggctgttga tcgccctgat catcaaccgc gaattccggg gtagagggtt
     7861 ggtgcgcaca gcgaccttgg ttccgtgggc gattccgaca gtcgcctcgg ctcagatgtg
     7921 gcggatgatg ttttccagtc agggtggggt gatcaacgat atagcgaaaa aactgggttt
     7981 gatcaccgac tatgtgaatt ttctaggaga cagtacgacg acggcttttt ggtcgatggt
     8041 caccgccgat gcctggaaaa ccaccccgtt tatggcgctt ctgcttttgg cgggcctaca
     8101 gacgatccct ttgcaactgt atgaagccgc gcgggtcgac ggcgcttcga agtggcggca
     8161 gttcttcagt attacgctcc cgatgctcaa acccagttta ctggtcgctt tgatctttcg
     8221 gaccctcgac acctttaagg ccttcgatat cgcgcaggtg ttgaccggag ggggaccggc
     8281 ttccggaacg gaattgttgt cccttttcaa ctataaattg atgatccaac aagggcagca
     8341 cggccgtggg agcgcggtgg cgattatgct gttcgtcttt acgatgctga ttgccgtctt
     8401 tttcattaaa gtcctcggcg ccgatcctta cgcctcaaag ggaagggaat tgaaatgaag
     8461 aagcggtgga tcccccgttt attgctgacc ttggccgtga tcgtgatcat cgtcttcatg
     8521 tttttccctt tctattggct gttcatcatg tctctgaaag gggatctcga tctctataaa
     8581 atgccgccgg actggatcgc gaagaatccg cgctgggata actataaaac gatcttcacc
     8641 gaccacaatc tcggtcagaa cctgcagaac tctttcgtga tcgccttttc taccacgctc
     8701 atcagcgtgt tgatcgggag ctgggcggcc tacgcgttgg ccaagttccg tatgcgtttt
     8761 aaaagcctca tcttgggcat cgtcttggcg gtttcgatgt ttcccggaat cgtcgtcgtc
     8821 tcgccgctct atatgcaatt ttcggacttg aagatcatca acacctattg ggcgctgatc
     8881 ttgccctacc tgaccttttc attgccgatg actatatgga ttctccaatc ctttttcaag
     8941 caaatccccg atgccttaac cgaagcgtct gtcattgacg gggcgggccc gatgcgtacg
     9001 ctattccaaa tcctcttccc gttggcagcc cccggcatct tcaccgcctt catcctcacg
     9061 tttatcggcg cgtggaacga gctgctcttc gcttcgatct tcatcaccga ctatcgaaag
     9121 ttcaccgttc cccgcgcgtt gacactcttt tcaggtcagt acacctcctc gtggcaattg
     9181 atagccgcgg cgagcgtggt gatcacctta cccttgatcg tgatggtact attgcttcag
     9241 aaatggatcg tcgccgggtt aacggccggc ggagtcaaag attgacgaaa ggagagcccg
     9301 agatggacaa gccctggtgg aagggagcgg ttatctatca aatctatccg aggagtttca
     9361 aagatgcgaa tcaagatggg atcggcgatc tcaagggcat aaccgaagag ttggattata
     9421 tccaagaatt aggggtggac gccatttggc tttctccgat cttcaaatcc ccgatgaagg
     9481 acttcggtta tgacatcagc gactatctgg agatcgatcc gatcttcgga acgatggccg
     9541 atttcgaccg tctgttggat gcggcgcacg aacgcgggat caaggtgatc ctggaccaag
     9601 tctacaacca cacctccgat cggcacccgt ggtttgtgga atcgcgcgct tcgagagata
     9661 atccaaaagc cgattggtac atttgggtgg atggagagaa ggggcaagcg ccgaacaact
     9721 ggctttccta cttcggcgga ggggcgtggg aatgggagga agcgcgtcaa cagtactatt
     9781 tgcacctctt cgtgaaagag caaccggatc tgaattggcg tcatcccgat gtccggaaac
     9841 aggtgatgga tagcatccgg ttctggctgg acaaaggggt ggacggcttt cgattcgatg
     9901 tggtcaacct gtactacaaa gacgagcggt tgcgagacaa tccgacaaaa tatccggacg
     9961 aaccgatcgc gtttaatcgt catcatcatc tctacacgcg cgacaggccg gagacggagg
    10021 gcgcgatcga ggagatacgg gagctgatgg accgctataa agaccgggtt tctatcggtg
    10081 aggtggcaac cgagctgggg caggcgcagt acttcgctta tacacggccg aatcgcttga
    10141 acctggcctt caatttcgat ttcaaagagc tgcggccctt cgcggccaag acttatgtcg
    10201 aacacatcaa gctcaacgag gcggtgtttc gggactttgc ctggccgagt tacgtgctgg
    10261 gtaaccatga ctcaaaacgg tttctgtcca aattgggtga aggcgaagac gccgtgcgta
    10321 aggcgaaggt cctcgccgct ctacttctga cgctgagggg aacgccgttc gtttattacg
    10381 gtgaagagat aggaatgcgc gaagccgtga tcccgtacga aaagatcgtc gatccgcaag
    10441 gcaaaaacct gtggccgaac aacgtcggtc gagatggatg ccgaacacct atgccctgga
    10501 acgcctctcc ccatgcgggg ttctctcaga ctgagccttg gctccccatc ggagaagacg
    10561 cgcgggaagt gaacgtcgaa cggcagaaag cggaaccggg ttcgatgttc cattattata
    10621 agcaactcat acgtatccgg aaagaatccg aggcgctgcg attcggtcgg ctggagctcc
    10681 tggacacgaa agatccggcg ttgatgggct tcttcaggat cggcgagtcg gaacgggtct
    10741 tagttctcct caatttcgaa gaccgcaagg tcgtattgaa tttgaaaggc tttggtgtcg
    10801 aggttcggcg ttacatagga ggcacaacat cgcgccccga gcgcttcaac gcagcttcca
    10861 cctctttgga gccttacgag gcggcgattt tggaagtcag ctgaaaatta cgcgttcgaa
    10921 aggtattctg tcttacgcaa accatagcca gatacccaag gcgatcatca gaagaccgct
    10981 gattgctttg aaaactttgg cgctcttttc ggtcatctta tacttcccca acgtcaccga
    11041 aaacccgacg acgatcagcc acaaaggaaa gatgtaggcg atattgtaga gaacgaggta
    11101 gaaatacttc gcgccgatgg cgattttccg ttctgtcagt attttcgaga ataacgccgg
    11161 taaccccatc gtacaggtga gttcgatcaa attgacgaat agggctaaag caacggtcag
    11221 cgcgatcgcc agcagcgtgc ttcgtgtttg gatgatcttt cgcacgcgtt gatacagctt
    11281 cggcttctgt ttttcggaga tcatcagaga gacccctttt ttgaagaaga agacctcttt
    11341 caaattgatg agccccatac cgatcgctac caccgagagg atccaggtga tgatccggtg
    11401 catcccgatg agggagaaga agttgaacca ggcggacata aagccgaagt aaacgacacc
    11461 ggaggcgatg acgaagaccg aaccgatcag aagcattcgc ttgcgcgatt tagccgtgga
    11521 aagcaggctg agcaggaaca tcaacaccca gaaagcgcag gggttgaagc cgtcgagcag
    11581 accgatcagg atggtcagga agaacaaagg cgagctccgg tcgatcttgc caaacagggg
    11641 aagcgtgatg gtgttgtcgg acgagactgg agcgacacca taggcggtga tgatggctct
    11701 gaggatttcg tctggcattg tcgtgcggtc aaaccccgaa aaggcctccc ggcctacgaa
    11761 aagaatggga attccggtga accgggtatc gtaagcagcg aaaaaggatt cgaggatgga
    11821 agcgttggcg gcgtcacggc tgatttcgta ggcgttcatt aaaagacggg gatcaacgga
    11881 ttggtattgg tcatagagcg cccggttgac cgtgtaccct tcgaactcct cataggcgtc
    11941 cgagtcgacc agcgtgttca aaaacgctcg gagctcttgg cagtgacgac aatcgtcacc
    12001 gtaaaaaagg tataaaaata cccgcccgtt ctccggctct cgccgaattt gaactgttga
    12061 gtccgtatgg acatcgagcc cttcccccag aatatcgagg agactgcatc ccccggattc
    12121 ttccgcgcac ggatcctcga tttgcccgag gcagaaagac aaagatgcga acgataagaa
    12181 cagaatgacc catagggccg cgaggtaggg tttaaggcgc tttaacgagt aaattgagcg
    12241 ggactgtggg attcgcggcc gcaaactcct gaacctcctg gacactggta ttcaacagga
    12301 tataataacc catccagtcg tatgtgggaa agagtttggc gaactcgctg aaggtcaccg
    12361 tccgaacatt cggtaacgta gccgtcagca gcgtcgcttc cttagacgtg accgatcgaa
    12421 tcagccgaac cggttgggac atcgcggggt tcattaccga gcgcaacgaa gtcacgtacg
    12481 actcatagct cagataccgt tcaaccgaac cgacggtttg aaattgggaa tccagcagaa
    12541 aggttcgcgg aatcgcagag acgtggaatt ttttcgccgc gatatcgtag tagctcaacg
    12601 tctcgtactg cgtaatatcc gggtttaaaa atacatcgaa aggaaagcgg gcatagaaat
    12661 ctttttccgc ctccgcaacg atgacgatgt gattcaaccg caacaagttc gctaattcca
    12721 gagtcgccag gccttccgcc ttcaattggg tgcaagaagg acagctgggg cgcgtaaaga
    12781 tcaacacgac ccccttctga gttaacgcgg cgaccttcag cccgttttcg atctcgttgt
    12841 acaccgcgta atcctggaat tgcaacgcta agagagaagt acacacaaat aggccgacaa
    12901 taaaagagaa caaaacccga ctcatccgaa tagcctcctt cgaaagcgtg gcgcgtattc
    12961 gtacgcgtga acgcgtgtac gcgaattata ccatagattt ccgagtcaat agggggcagt
    13021 gagggtcaag ttatgaggcg ccatcaagaa acaaggccag aatcgctcgc gcgattttta
    13081 gtccataaag ccatcgcagc gtaagcgttt aagggttctc ggaagcttcc agaaaagcaa
    13141 aaaaacatgc cgaactaaag ttcggcgttc ccagggcgga tcgcgcggct tcgtgggctg
    13201 ggaacgccaa cttttaagtt ggcatgtctt gaaaaaagtt catacggaga cggcggaggg
    13261 cttataggag agagatgttt gcaagagtca acaccaggtt tatattttct ctgcgctctc
    13321 tgtgtcctct gtgcgaaacc ccggctttaa accatgccga actaaagttc ggcgttccca
    13381 gagcgcatcg cggggtcgca aactacactt accttgcgat aaccccacag gctcattgcg
    13441 gtgccccgat ggtcatcgcg gcgtaagcgc ttcttctttt cgcgatggcg cgatacgctt
    13501 ttgtacgggc taatcgcgag gcttcatcag tatcgcgaga cttcgtgggt ttccgaagtc
    13561 agcccgctgg ttatcccgcg cttaccccgc acgggcatcg cggcgtaagc gcttcttctt
    13621 ttcgcgacgg cgcgatacgc ttttgtacgg gctaatcgcg aggcttcatt agtttcgcga
    13681 gacttcgtgg gtttccgaag tcagcccgct ggttaccccg cgataaacgt cgttgtcttc
    13741 gcaagttcgt aaactacact taccccgcga tgaaccttgt agttatcccg gcgtaagcgt
    13801 ttaagggttc ttggaagctt caaaaaaagc aaaaaacatg ccgaactaaa gttcggcgtt
    13861 cccagagtgg cgttctgagg tgctctagca aacgaaaaaa accctgccga actaaagttc
    13921 ggcgttctca ggcgctcttt tacgaggttc gtgttataat tttttattga tgagcgcatc
    13981 ggaggggcta tgtttgacat taagggttac atattcgatt tggacggggt catcgttgat
    14041 acggcccgtt ttcatttttt agcttggaga gaaaccgcgc gagaattggg gtttgaactg
    14101 accgagcgcg ataacgagcg gctaaaaggg gtgagtcgga tggaatcgct cgagatcatc
    14161 ctccgattgg ccggcattca tttggacgaa gagcgtaaat gggctttcac cgagaagaag
    14221 aatacacgtt acctggagtg ggtcgacacc cttcggcctt cggacacctt acccggcatc
    14281 gccgacttcc tccggtctct taaggaagcc cacaggcccg tcgcactggc ctccgcgagc
    14341 aaaaacgccc ggcgggtcat ctctctgctg gagatcgcct cgctgttcga tgcggtgata
    14401 gacggttcga tgatcgaaaa gaccaaacca gaccccgaga tatttatcaa agccgcccaa
    14461 gcgctgggaa ttcgcgcctc tgagtgcgtc gtttttgaag acgcccaaaa cgggatagag
    14521 gcggcaaaag cggcagagat gaccgctgtc ggtgtggggt ctcccgagca cctcggagag
    14581 gcggatttgg tgattcccga tttctctcct tcgcacatct atctcttaaa acagaggggg
    14641 atcgtcagat gaatgagatc gtctttaccg aagaccactg ggatccgtca atttatccgt
    14701 tgcacgaaac gcttttcgcg caagcgaacg gatacttggg gatcagaggg agttttgagg
    14761 aagggttcgc ggggccttct cccaaaagcg tccgtggaac ctatatcaac gggttttatg
    14821 agaatgaaaa gatcctttac ggggagtccg cttttggatt cgcgaaaacg aatcagatta
    14881 tgctccatat tccggatgcc acacgccttc gtttgacggt ggatggtgaa cccgtcttca
    14941 tcgacacagg gcggctatgg gcctacaagc gagtgttgtc tctggcggat ggggtactaa
    15001 ctcgaacaat ggactgggag tcgcctctag gaaaacgggt tctcttccgc tttcgtcggt
    15061 tcgtatcctt gcaggaccct cacctgctgg cgatgcatct gacgctgaca ccgatcaact
    15121 tttccggggc gatagaactc gtttctctca tcgacggaaa cgtccggaat atacagaacg
    15181 aagatgatcc ccgtgtggga agccagtcag aggggctttt cttagaaccg atttcatcgg
    15241 ccggctacgc gaaagagggc ttggccatct ctgtgtttcg aacgcgccac tcgaacctca
    15301 ccttaggctg ttccatccac catctgttgt ctcgagaacc ggctgaggct cccgaggtca
    15361 cgtgcgagaa tcggcaagcc gccatccgat atgtcctgct cctcgatcca gagcaagaag
    15421 cgtcgttgac gaaattcgcg tgctacacca cctcacagga gacgccggtc gaagagatcg
    15481 accggttttt gcggcagaag ggagcccacg cgctccaaga aggatttgac gcgtacgcgg
    15541 cttctcaagg cgaaatcctg tcccgttact ggcgtgttgc cgaggtgaag attgaaaatc
    15601 agcctgaggt cgagaaggcc atggctctga atcagtttca cctgtaccaa tccacgggga
    15661 aggacggaaa aaggaacgtc gcttcgaagg ggctgaccgg cgagggatat gggggtcata
    15721 ccttctggga cacggagatc tatatgatcc
//
LOCUS       DAOPXN010000040        15542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3864_length_15542_cov_5.149351, whole genome shotgun sequence.
ACCESSION   DAOPXN010000040 DAOPXN010000000
VERSION     DAOPXN010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3864_length_15542_cov_5.149351"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            299..676
                     /locus_tag="PKM49_03630"
     CDS             299..676
                     /locus_tag="PKM49_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81935.1"
                     /translation="MKRALCVMVLSLIAITAVLAAPLSRTIVIGTETLAFTESQFSSL
                     LVRYEKEAAPEECGVILKLFLKSGPYHYLSKVVKLTFPKYFSLPRPDYDLQILKIRYH
                     IEQRIQTFMEGRETVLHINLSDF"
     gene            complement(763..1692)
                     /locus_tag="PKM49_03635"
     CDS             complement(763..1692)
                     /locus_tag="PKM49_03635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY81936.1"
                     /translation="MVEFRLARSTELLQVFSVINQVFRSKYDPTMQLEFPLLFDLKNL
                     ARVFVAVEEGRVVSHLAYQRTDYLSEGRRVCVGFLGAVATLPEHRGSGIASRLLEMAE
                     EAMHQEGVDLCLISGNRPLYLRQGYEERGVTYEYPLTAQNKSPEVRVRVYTEADLDTV
                     LELYAGEQKLFRREPEDFRRLLDVNPTLKAQGPRSIPISQIGPTFLVYDQETPIAYFI
                     LRGSEKYPIEYAGDRLGVLRGLQTLITETGRPSFIWVPQSDDVMLRLLEREGYQIQTR
                     YHFPPYFTAKVLNAAACDENVWLGMPLPGINYV"
     gene            2754..3071
                     /locus_tag="PKM49_03640"
     CDS             2754..3071
                     /locus_tag="PKM49_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HNY81937.1"
                     /translation="MNKGDSPFLFENIMELVVDEVIEDLLKVPTLQVCRCDRCYIDIK
                     ALALNRLPPKYVVTERGEIFAKLGTFRNQVRVDVLKAVIEAIEIVRQRPSHSALSPVF
                     KKE"
     gene            3219..4157
                     /locus_tag="PKM49_03645"
     CDS             3219..4157
                     /locus_tag="PKM49_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81938.1"
                     /translation="MKRRLLLVNLLFITVYLFSYKIALPMGPTLVSVLPITEGRIAPE
                     GNYEFVHWKSFDEAIAMVASRQVSAILLPVSYGATLHDKGLPVKLVAVTLWNSFFCVS
                     DGWDYTGIASLRSQTLYFAQGKGQTADVVLRILLKAKGLDPDRDLSMKYLSPPEIVAF
                     FQKGLATLAIVPEPYASLILNLKPKASIAFDLQTLWQETLKTAFRLPVTGLFYVPAAN
                     EPPSQTKDQVNTLRQDFQKATGFLSNHLEEMAALATSVFPGMAEGVILRSIGNSEYDF
                     SFSEEDKKALIHYFDTISRFLPESLPAVPDEDFYLF"
     gene            4135..4914
                     /locus_tag="PKM49_03650"
     CDS             4135..4914
                     /locus_tag="PKM49_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY81939.1"
                     /translation="MRTSICSSLFKYGLGLFLIALGWELASLWFRSSLVLPSLSEVFA
                     AFFGLLNDPSLWMHLSSTFFKAFFGLFLALAIGLPLGVLIGLSAFFSQLLLPALVLLK
                     SMPIISWLTSILILWGIGWKAPVFIVLVTLLPIVIYHAANGIKTVDKQLLELVKLNNL
                     PFRKAFSGIYLPSIVPFIVSSVEISIGTMWKTVVAAEFLAGEKGIGVQIAWNKYYVQT
                     PVVFAYTLFVIIIGLGLEILLNAFFSSPFMRRWRGLWSTSY"
     gene            4896..5594
                     /locus_tag="PKM49_03655"
     CDS             4896..5594
                     /locus_tag="PKM49_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY81940.1"
                     /translation="MEHVVLKGIHKAFGRLRVLDGIDLVVERQHAVALLGPSGCGKTT
                     LIRMVAGLEKPDRGEIATLPGGVSFAFQEPRLIPWKTVRENLTFFLPEPKIDSSFLDA
                     FELTEILDAYPHQLSGGLKQKVNIGRALLLNKPLLLLDEPFQNIDVNTRVSYIRSITE
                     RRKEEGFTMILITHNLREALLFADRLVFFGPKPARIVGEWLVKRPPDQSFRSFDWLFE
                     EERKVVALFPEFMI"
     gene            complement(5656..7410)
                     /locus_tag="PKM49_03660"
     CDS             complement(5656..7410)
                     /locus_tag="PKM49_03660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HNY81941.1"
                     /translation="MQWDLTFFYKDHQDPNLVKDLKEALKESEEYQTEGVKFFSKPKI
                     DAKGLEKLFKTQEKIIQKIARAGEFSFLYFAQNTANLEAQKLLGLCANYEAKIRQNFA
                     FITPSLARLSDDALKELETEIPAYRHVIEKVRIGKANTFSVETEQVLAARSNSGRGRL
                     SELYDKIVSAFSFSLTVDGEKKTLTGEQVRNLRYHPDSKTRRKAMKVYFKKYEENQIA
                     LANLYNIIQTDYDTEASLRKYPSSISMRNRENETDDRTVGKLIEVTTDNAILVERYYR
                     WKGKKLGLKMTPADIYAPLETVKKTYTYEEAKQIVLDAYLHFDRDIGKIVESFFEERR
                     IDSKIVPGKRAGAFCSYAYPHGKPFILLNFDGNIREIMTLAHELGHGVHGTLSAKQSY
                     LNFFTPLTMAEIASVFGEMIVFDKLMGELDAESQMLFLARTIEDMFATTFRQNMFARF
                     EVSSHRRITEEGFVPWEELCELYKGELQKMFLTSLTIPEEYHAEWSSIPHIYGVPYYV
                     YAYNFANLLVLSLYGKYKEEGKRFIPKYKALLASGGSDHPEKLLAPLGIDLTSETFWQ
                     KGFDIVKGYIDRLESLSV"
     gene            7578..8714
                     /locus_tag="PKM49_03665"
     CDS             7578..8714
                     /locus_tag="PKM49_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HNY81942.1"
                     /translation="MIETFLSFFATTAFGAVSFGNLIMIGIATVVLIVSIKKDAEPLL
                     LIPIAFGMLLANIPILHTGLFAKPTDTTTGGLMYYITMGLQLEIYPPLIFLGIGALTD
                     FSFLLSNPILIFLGGAAQVGVFIAFGLAKAFGFTLPVAAAIGIIGGADGPTSIYIAKR
                     FAPEYIAMIALAAYSYIALMPLLQPPIMKALTTKKERMIRMKPPRQVKKVEKLVFAGA
                     TVLFCGLLVPPSIPLVGMLMLGNFLRESGYTKRLAEACSRYILDTATIMLCLSVGGSA
                     KAEVFLQPTSLLILGLGCVAFITSIAGGVLFTKFMNLFLKEKINPLVGGAGVSAVPDS
                     ARISQQVAKQEDPTNFILMHAMGPNVAGVIGTAVAAGVFLSVLG"
     gene            8719..9828
                     /gene="alr"
                     /locus_tag="PKM49_03670"
     CDS             8719..9828
                     /gene="alr"
                     /locus_tag="PKM49_03670"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNY81943.1"
                     /translation="MDVLNSRRTRACISLTAYRRNLLYFSEQGAPSLIAPVIKADGYG
                     HGAIPLAKVAADLGCPYLCVGFLEEALQLRYAGVTLPILVFNYFAPETTPLFIQENLT
                     ATVYSGYQLEAIAPYIAGGKLRAHLKVDTGMSRLGVKPEEVERLIRRVWEIGNIELEG
                     LYSHFATADDPDSDFARRQYEQFAALRRLYRSSIRYFHISNSAGAQYLSVDPFDFIRL
                     GISSYGLDARNQKRPSELSPVLSWETILSHVKTIREGASISYGRTFIADRPMTIGTVA
                     VGYADGYNRLLSNRGSVLVKGRRCPILGRVCMDQFMIDLSGIPAPEPGEKVTLLGFDG
                     AEEISCEEIAALLSTINYEVTCLISKRVPRVYTEV"
     gene            9874..10962
                     /locus_tag="PKM49_03675"
     CDS             9874..10962
                     /locus_tag="PKM49_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY81944.1"
                     /translation="MNLGELVMKKIAELSEKTGQFNELLDGKLVSDIRIDDQVSTILK
                     VVSKKLQNTRDGKTFLLMTLGDRSGTIRSIDWNNSKYNDERLTNGTVVRATGKVSLFE
                     GHPQLNIASSRDAIKVLGEGEFDPNKFIEITSKNIREMYDSLLKQIQKVKNDKVRALL
                     HEAFVKNDRFVSDFIESPAAINVHHAYKGGLLEHTLDVTELAIQLMEAYPEMPIDKDL
                     VIAGALLHDIGKVEEYSLSPTGIDRSDAGELIGHIVIGINWLTETAARIPQFPKKALV
                     DLQHILLSHHGEIEWGSPVLPKTIEALIVHSADNTDSKIAQFREYTLKAQPNILNPDD
                     TVWSDFNKYLSRRIRIRGVVSYGEKTKD"
     gene            10940..12391
                     /locus_tag="PKM49_03680"
     CDS             10940..12391
                     /locus_tag="PKM49_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HNY81945.1"
                     /translation="MEKKRKIKFGTSGWRGILAEDFTFDNVRTVSQAIATEILKDPKA
                     KNGALVGYDSRFLAEDFAWAVAQVFAGNGIRTYVCHQPTPTPVISYTIRNLNLAGGVN
                     ITASHNPHFWNGIKFNPATGGPALPEVTKAIETEIERLQRGEGEVRLLTHLDEEFSKL
                     VYSIDPSQEYFAEIRRKIDMDRIKGAVKKVAVDLVYGTGVDYLDQLLLSLLPGEEAQG
                     LVVLHDYRDPYFGGYRPEPDKKRLRELGQVVLEHRAQIGLAVDGDADRFGIVDDKGVP
                     ITANEYLAIVAWHLYKNKGLRGDSARSVATSHAMDRIVEHFGFQTLVTPVGFKFIGDA
                     ILNDRIVLGGEESGGLSIQDHVPEKDGILACLLALEAIAYEQRPMSEIRKIVQEVTGP
                     YFTDRIDLELESESRKRELMDFFANMQREVFGIPVKERQTVDGIGFDLSIPEGEAWIL
                     IRASGTEPVIRIYIESTQEKVFLKLQEGVEKLG"
     gene            12388..14679
                     /gene="topA"
                     /locus_tag="PKM49_03685"
     CDS             12388..14679
                     /gene="topA"
                     /locus_tag="PKM49_03685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNY81946.1"
                     /translation="MKTLVIVESPSKAKTIEKYLGKDYHVISSKGHIRDLPPDELGVD
                     IEASFEPKFTLMRGKEKVVQQIKEEARNKPVLLASDMDREGEAIAWHLAQILSLSDQE
                     TNRVLFSEITEKAIKAAIKRPLRINRSKVEAQFARRILDRLVGYQISPFLWKVIQVKS
                     LSAGRVQSVALRFICDLEEKILTFVPEEYWKFKARFLCEKMQGELETALVSVDGVSLE
                     KQPIRNVQDAMKIEEEILKSSFAVRSVEEKVINRKPPAPFITSTLQQEASNRLGFSVS
                     RTMKVAQELYEGLETKDGPLAFITYMRTDSNRISEEALEAAKLYITEHFGESHSKTQR
                     YGKNKANTQDAHEAIRPTYPDVDPQKAEKMLSADQAKLYRLIWSRFMASQMIPAVYRQ
                     TKIKIEDIEQGCYTFEAEGARCVEEGYEKVYPPTSKEKDLSVVPVAGTPASLSHFEKA
                     QNFTTPPARFTEASLVKKLEKEGIGRPSTYASIIHTLLERHYAYREQKSLVPTFLGMI
                     VNHFMSDFFPKIIQAKFTASMEENLDKVEEGEENWKEAIQRFYSDFEPTLLKLKEKTL
                     TKSLNWLFSTNLPCKENEKHTLILKMGRFGPYLRCEACDKNYSISEKAKIIFRQNQAE
                     IYGETVLENSKEVVAIGRACPKCGGDLVERQGRYGKFIACSNYPKCKYTENVDEKVAA
                     KCPKCGADVVRKKSKRGRTFFACKNNAYNGGTCDFFSWYEPTEEKCPQCGQTMVLKKG
                     RSGEESIVCESCARTTRIAKKTK"
     gene            14809..15276
                     /locus_tag="PKM49_03690"
     CDS             14809..15276
                     /locus_tag="PKM49_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81947.1"
                     /translation="MTDKIAITIGEKALSYWLRIMLAQKVEKIQDVEVSCELQGFQLV
                     VRVGIPGFNAGVQAIFRLLQELRDEGGIHTLLFSVKPVGLFSSMALATFSKAFDRLKP
                     GIVFADNTLRVSFRDLLAPNGPLQAPLLEGYSIESIDFLPSTFSIVLKLKATS"
BASE COUNT         3812 a         3946 c         4056 g         3728 t
ORIGIN      
        1 agtattttga aagactgaag tcccttacaa tagagcaact tgactccttg ggacttgagt
       61 tgatcgatat gaaaaacgag gaagagttaa agaagcattt aatgtagaga gtccgacaaa
      121 aggcttgaac gcgatttgat ggatcgtgtg cgcgaagtaa tggtggctcc tttgggggcc
      181 gttatttccc acctcgtttt tgtctttttt cttatcatac cccgatataa tacccacaca
      241 ccgcggccaa taaaaaacag gggctgtggt ataatcggaa aaaatgagga ggaataatat
      301 gaaaagagcc ctttgtgtga tggtgttgtc tttgatcgca ataaccgccg ttctagccgc
      361 gccgctctcc cgtacgatcg tgatcggcac tgagacgttg gccttcactg agagccagtt
      421 ctcaagcctg ctcgtccgat acgagaaaga agccgccccg gaagaatgcg gtgtcatcct
      481 caaactgttt ttgaaaagcg gcccttacca ctacctttcg aaagtggtga aactgacctt
      541 tccaaagtac ttttctctcc ctcggccgga ctacgatctg caaattctca aaattcggta
      601 ccatatagag caacgcatcc aaacctttat ggaaggccga gaaaccgtct tgcatatcaa
      661 cctgtccgat ttctaaggca gggaccacct cgggccaaac tgccgtggga gatctcggct
      721 cgacgccgcg cgacccgctg ctctttcggt tgagtccctt ttctaaacgt aatttatacc
      781 gggcaaaggc ataccgagcc acacgttttc gtcacaagcc gctgcgttca ataccttcgc
      841 tgtaaagtac ggcgggaagt ggtaacgggt ttgtatttgg tacccctctc gctccaatag
      901 ccttagcatt acgtcgtctg attgcggaac ccaaataaag gacggacgac cggtttctgt
      961 tatcagtgtc tgcaaaccgc gcaacacccc taagcgatct ccggcgtatt cgatcgggta
     1021 cttctcgcta ccgcgtagta tgaaataggc gataggcgtt tcttgatcat agactaaaaa
     1081 ggtcggacca atctgagaga tcgggatcga gcgtgggcct tgcgccttta acgtcggatt
     1141 cacgtcgaga agcctccgga aatcttccgg ttctctacgg aacagtttct gttctcccgc
     1201 gtataactcg agaaccgtat cgaggtcggc ctccgtatag acacgcactc gaacctcggg
     1261 agacttgttt tgagccgtca atggatactc ataggtcacc ccgcgctctt cgtagccttg
     1321 gcgtaaataa agaggtcggt ttccggaaat gagacaaaga tcgacccctt cctgatgcat
     1381 cgcttcttcc gccatctcca gcaaccgaga agcaatgccg cttccgcggt gttcgggcaa
     1441 ggtcgccacg gcccctaaaa accccacgca gacacgcctg ccttccgaaa gatagtcagt
     1501 tctttgatag gccaggtggg agacgacgcg accttcctcc accgcgacga agaccctcgc
     1561 gaggtttttt aaatcgaata gcagcgggaa ttccaattgc atcgtagggt cgtatttgct
     1621 acgaaaaact tgattaatga cggaaaaaac ctgtagtagt tcggtagaac gcgcgagtct
     1681 gaattcgacc attccgatcc cccttccctt ttgtatggtg tgctgtgaaa acaaaaactc
     1741 cataacgtct tctgtcggct ttccgaactc actaggctcc catcgatccg cgattcgtca
     1801 aaaatcattc gttagtgcct tggagagtgg gcccctcaaa cgcgaaaagc gaagcgtgtc
     1861 ttcgatcgat caaaacgcga aggcttccgc gaacccaatt atagcatgaa cagcgagtga
     1921 cggggcgtgt tggtgtgggt caagttttgg ggagtactca agaaacaagg ccagaatcga
     1981 tcgcacgatt tttagtccac atagtcatcg cggcgtaagc gacccttctt ttcgcgacgg
     2041 cgcgatacgc atttcaaaag aagggttcgc acggagggcg cgctgggaac gccaactttc
     2101 aagttggcac ggtttctaag ccgttaaaaa gacaaaaaga agggttcgca cggagaacac
     2161 agagggcaca gagaaagaaa aaagaagggt tcgcaccgag ggcacagagg gcacagagaa
     2221 agatgaaaac aagaagacat gccaaactga aatttggcgt tcccaggagt tcccagaaaa
     2281 gccagaacaa cagacaaaag caagaagata tgccggactg aagtccagcg tttctaggcg
     2341 ttccaaagcg gggaatcgcg gggtcccgtt ggttaccccg cgataacccc acacgatcat
     2401 cgagacttcg gtgcttccga agttagtccg ctggttaccc cgcgatgatc ctcttagtta
     2461 tgtcgcccca taacttgaca cacgaggcgt gttggcgagc accttttctc cgagaccttg
     2521 ggtgggatga taaaaaccga gttgtcattt acgcttatgt gacctccttt ttccctcgtc
     2581 attttactgg cctacctcta gcggttcaac agaatccaca catcgtgtta ctgttaactg
     2641 acgtttaaaa aatgagaacg aatcgacctt atgctcgtga aatgtccgaa attcccaatt
     2701 ttatggtaat atcggaaggc gcagaaattt cggaaactcg aggcggtgac caggtgaata
     2761 agggcgattc tccttttcta ttcgagaata ttatggaact cgtcgtggat gaagtcatag
     2821 aagacttgct caaagtgccg actttgcagg tgtgtcggtg cgatcggtgt tatatcgaca
     2881 tcaaagctct cgctttgaac cgattgcccc cgaaatacgt agtcacggaa cggggagaga
     2941 tcttcgccaa actcggtacc ttccggaatc aagtgcgagt ggacgtgctg aaagccgtga
     3001 tcgaagcgat agaaatcgta aggcagcggc catcccactc ggctctcagt ccggtgttta
     3061 aaaaagaata agagccctcg agaggggcgt gcaacagaga cggttcgccc cttttttcgg
     3121 acgaagcgtt tcgacaatga actcttgcca caccgcccat agcaattttc aacaacaaga
     3181 gctagggcta tccaaacgtc aaagaggaac gataacggat gaaaaggcgc ttacttttag
     3241 tgaatcttct ctttatcacc gtatacctat tttcttacaa aatcgccctt ccgatggggc
     3301 cgacactggt ctccgtcttg cctatcaccg aggggcgcat cgcccccgag gggaattacg
     3361 aattcgtcca ttggaaatcc tttgacgagg cgatcgcgat ggtggcaagc cggcaagtct
     3421 ccgcaatact cctaccggtc agctacgggg caactctgca cgacaaaggg cttccagtga
     3481 aactcgtcgc cgttacctta tggaatagct ttttttgtgt ctcagacggg tgggactaca
     3541 cgggtatcgc gtctttacgg agccaaaccc tttacttcgc gcagggtaaa ggccaaacgg
     3601 ccgatgtcgt cctgcggatt ttgttgaagg cgaagggcct ggatccggac agggacctct
     3661 cgatgaagta cctctcccct ccggagatcg tagccttctt ccaaaaaggg cttgcgacgc
     3721 tcgcgatcgt tccggagccc tacgcttcgc tgattttgaa cctgaagccg aaggcatcga
     3781 tcgccttcga tctgcagacc ctatggcaag agacgttgaa aacggccttc cgcctaccgg
     3841 tcaccgggct cttctacgtg cctgcggcga acgagccacc ttcccaaacg aaagaccaag
     3901 tcaacaccct ccggcaagat ttccaaaagg ccacagggtt tctttccaat catctggaag
     3961 aaatggccgc gctggcgacc tctgtcttcc cgggtatggc cgaaggggtt atcctccgtt
     4021 cgatcggaaa ctccgagtac gacttctctt tttcggagga agacaagaag gcgctcatac
     4081 actacttcga cacgatttca cgatttctac ccgaaagttt gcccgccgtt ccggatgagg
     4141 acttctatct gttctagtct cttcaagtac ggcctaggct tgttcctgat cgcgttgggt
     4201 tgggagttgg cttccctctg gtttcgttct tccttggttc tcccgagtct gtcagaggtg
     4261 ttcgcagcct ttttcggatt attgaacgac ccgagcctat ggatgcatct ttcttcaacc
     4321 ttcttcaagg cctttttcgg gttgttcctc gctttggcca taggccttcc cctcggcgtt
     4381 ttgatcggtc ttagcgcctt tttttctcag ttgctcttac ccgcccttgt gttgttaaag
     4441 agtatgccga tcatctcctg gctgacgagc atcttgatcc tttgggggat cggttggaaa
     4501 gctcccgtgt tcatcgtttt agtcaccttg ctaccgatcg tcatctatca cgccgcgaac
     4561 gggatcaaaa cggtggacaa acaactcttg gaattggtga agctcaacaa cttgcccttt
     4621 aggaaagcct tttccggtat ctacctaccg tctatcgtac cgttcattgt ttcctccgtt
     4681 gagatcagca tcggaacgat gtggaaaacc gtggtcgcgg cagaattctt ggcgggagag
     4741 aaaggtatcg gagttcaaat cgcctggaac aagtattatg tccaaactcc cgtcgttttt
     4801 gcctacaccc ttttcgtgat catcatcgga ttgggattgg aaatcttgtt gaacgcgttt
     4861 ttttctagcc cgtttatgag gcggtggcgt gggttatgga gcacgtcgta ctgaaaggca
     4921 tccacaaagc gttcggccga ttgcgggtcc tagacgggat cgacttggtt gtcgaacggc
     4981 aacacgccgt cgccctattg ggtccttcgg gctgcggtaa aacgacgctc attcggatgg
     5041 tcgccggtct cgaaaaaccg gatcgtggag agatagcgac cttacccggc ggagtcagtt
     5101 tcgcctttca ggaaccccgc ttgatccctt ggaaaaccgt gcgggaaaac ttgacgttct
     5161 ttctaccgga acccaaaatc gattcgagct tcttagacgc tttcgagttg acagagatct
     5221 tggacgccta tccgcaccaa ttgagcggcg ggctcaagca aaaggtcaac atcggaaggg
     5281 cgctgttgct taataagccc ctgctgcttc tggatgagcc ctttcaaaat atcgacgtga
     5341 acacgcgggt ttcctatatc cgctcgatca ccgaacggag aaaagaagaa ggcttcacga
     5401 tgatcttgat cacccacaat ctacgagaag ccttgctttt tgccgatcga ttggtcttct
     5461 tcggacccaa acccgcgcgt atcgtcggtg aatggctggt taaaagaccg cctgaccagt
     5521 ctttccgctc tttcgactgg ctgttcgaag aagagaggaa ggtcgtcgcg ctatttccgg
     5581 aatttatgat ttaaaaagtt ggatatcttc ccaacgggcc cctcgtttct ttccccgggc
     5641 ccgtaggtaa gtcgtctata ccgaaagcga ctccaaccta tcgatgtacc ccttcacgat
     5701 atcaaaacct ttctgccaga aggtctcgga ggttaagtcg atgcccaagg gagcgagcag
     5761 tttttccgga tgatcgctac cgccactcgc caataacgcc ttgtatttcg gaatgaaacg
     5821 ctttccctct tccttgtatt tcccatatag cgacaaaact aagaggttgg caaaattata
     5881 ggcgtaaacg tagtacggga ccccgtaaat gtgtgggatc gaggaccatt ccgcgtgata
     5941 ctcctcgggg atggtcaggc tggtcaggaa catcttctgc aattcgcctt tatagagctc
     6001 acagagttct tcccacggta cgaaaccttc ctcagtgatg cgccgatggg aggacacttc
     6061 gaaccgcgca aacatatttt gtcggaaagt ggtggcgaac atatcttcga tcgttcgcgc
     6121 tagaaagagc atctggctct ctgcgtccaa ctcgcccatc agcttgtcga agacgatcat
     6181 ctccccgaag accgaagcga tttcggccat agttagcggg gtgaagaagt tgagataaga
     6241 ctgtttcgca gagagggtgc cgtgtacccc gtgacccaac tcgtgggcca aggtcataat
     6301 ctcgcggata ttcccatcga aattgagcag gatgaatggc ttcccatggg gataggcgta
     6361 agaacagaat gccccggctc gcttgccggg aacgattttg gagtctattc tccgctcttc
     6421 gaagaagctc tcgacgatct ttccgatatc tcgatcaaaa tgaaggtatg cgtccaacac
     6481 gatctgcttc gcttcctcat aggtataggt tttcttgaca gtttccaatg gcgcgtagat
     6541 atccgccggg gtcattttca aaccgagttt tttccctttc caacgatagt agcgttcgac
     6601 caaaatcgcg ttgtccgttg tcacctctat caattttccg accgttcgat cgtcggtttc
     6661 gttttcgcgg ttgcgcatag aaatgctgct agggtacttc cgtaaagagg cttccgtgtc
     6721 gtaatccgtt tgtatgatgt tatagaggtt ggcgagcgcg atttgattct cctcgtactt
     6781 tttaaaatac actttcatcg ctttcctgcg ggtttttgag tccggatggt aacggaggtt
     6841 tctcacctgc tcaccggtga gcgttttctt ctccccgtct accgtgagag aaaaggagaa
     6901 agcggaaacg atcttgtcgt aaagctccga caggcgaccc cggccggagt tggagcgagc
     6961 cgccaaaact tgctcggtct caactgaaaa ggtattcgct ttgccgattc gaactttttc
     7021 gatgacgtgc cgataggccg gtatctctgt ctcgagctct ttaagcgcgt catctgacaa
     7081 tcgagcgagc gacggggtta taaacgcgaa attttgcctg attttcgcct cgtagttcgc
     7141 gcataggcct agcaatttct gcgcttcgag gtttgccgtg ttttgggcaa aatagagaaa
     7201 ggagaattca cccgcgcgtg cgatcttttg gatgattttt tcctgagttt tgaatagctt
     7261 ttccaagccc ttcgcgtcta tcttaggctt tgaaaagaac ttgacgcctt cagtttggta
     7321 ttcttcggac tccttgagcg cttccttcag atcctttacc aagttcgggt cctgatggtc
     7381 tttgtaaaaa aacgttaaat cccactgcac ggggactcac ctccggaata tggattatac
     7441 gtgactattc tatacgactc gcaagccttt ggcaagtgag gttctttttc ttgggttttg
     7501 tgataaaatc ttaccggccg tagacagccg gttcaaaacg agaatacgcc ggtctgcgtt
     7561 ggagggatga ctctttgatt attgagacct ttttgagttt ttttgcgacc actgcctttg
     7621 gagccgtcag cttcgggaat ttgattatga ttggtatcgc cacggtcgtt ctcatcgtat
     7681 cgattaagaa agatgcggaa cctttgctgc taatcccgat cgcgttcgga atgcttctgg
     7741 cgaatatccc gatcctccac acgggtctgt tcgccaagcc gaccgataca acaaccggcg
     7801 ggttgatgta ttacatcacg atggggttgc agttggaaat ctatcctccg ctcatctttt
     7861 taggaattgg cgcgctcacg gacttctcct tcctgctgtc taacccgatt ctcatctttc
     7921 taggcggtgc ggcgcaagta ggggtgttca tcgctttcgg gctggcgaag gcctttggct
     7981 tcaccttacc ggtagcggca gcgatcggta tcataggcgg cgcagacgga cccacctcga
     8041 tctacatcgc caaacgcttc gcgccggaat acatcgcaat gatcgccttg gccgcctact
     8101 cttacatcgc tttgatgccg ctcttgcaac cgcctatcat gaaagccctt acgacgaaga
     8161 aagagcggat gatccggatg aaacctcccc gacaggtaaa aaaggtggaa aagctcgtct
     8221 ttgcgggcgc caccgttctg ttctgcgggc tgctcgttcc tccgtccatc cctctcgttg
     8281 ggatgctgat gctggggaat ttcttgaggg agagcggata caccaagcga ttggccgaag
     8341 cctgctcgag gtatattttg gacacggcga ccattatgct ctgcctatcg gttggaggct
     8401 cggccaaagc cgaagttttt ctgcagccca cctccctatt gatactcggt ttgggttgtg
     8461 tggcattcat caccagtatc gccggcggcg ttcttttcac gaagtttatg aacctattcc
     8521 tcaaagagaa gatcaaccct ctcgtcggag gggccggggt ttctgccgta ccggattccg
     8581 ccagaataag ccaacaggtc gccaaacaag aggacccaac gaattttatc ttgatgcatg
     8641 cgatgggccc gaatgtcgcc ggggtcatcg ggacggccgt tgcggcggga gtctttttgt
     8701 cggttcttgg ataatcgtat ggatgtcctc aattccagac ggacgcgagc gtgcatctcc
     8761 ttaacggctt accgccggaa tctgctatat ttctctgaac aaggggcgcc gtctttgatc
     8821 gcccccgtta tcaaggcgga tgggtacggc cacggagcga tccctctggc gaaggttgcc
     8881 gccgatcttg gttgccccta tctgtgcgta gggtttttgg aagaagccct gcaattgcgg
     8941 tacgccgggg tgaccctacc tattttggtg tttaactatt tcgctccaga aacgacaccg
     9001 cttttcatac aagaaaatct cacagcgaca gtctattccg gctatcagct cgaagccatc
     9061 gccccatata tcgcgggagg taaactcaga gcgcatctga aggtcgacac cggtatgtct
     9121 cgtctcggtg taaaacccga ggaggtcgaa cggttgatac gtcgagtttg ggagatcggt
     9181 aatatcgaac tcgaaggttt gtacagccat ttcgccacag ccgatgatcc ggattcggat
     9241 ttcgcgcggc ggcaatatga gcaattcgcc gccctcaggc gactctaccg ctcttcgata
     9301 cggtattttc acatctcaaa cagcgcgggc gctcaatact tgtccgtcga tccgtttgac
     9361 tttatacggc tcggcatctc ctcatacggg ctggatgcgc gaaatcagaa aagaccgtcg
     9421 gaactctccc cggttctttc ctgggaaacg atcttatccc acgttaagac catacgggag
     9481 ggcgcttcga tcagttatgg gcggacgttt atcgccgacc gaccgatgac gataggaacg
     9541 gtagcggtgg gttacgcgga cgggtacaat cggttgttaa gcaatcgcgg gtcggtgttg
     9601 gtcaaagggc gcagatgccc gatcttgggc cgcgtgtgta tggaccaatt tatgatcgac
     9661 ctctcgggta tccctgctcc cgaacccggc gaaaaggtga cgctcttagg gtttgacgga
     9721 gcggaagaga tctcgtgcga agagatcgct gcccttcttt ccacgatcaa ctacgaagtg
     9781 acctgcttaa tctccaaacg agtcccccgc gtttacacgg aagtataagc gaatcgaaca
     9841 tttcgcatat aaaatacgag aggagttttg tgtgtgaact taggcgagtt ggtgatgaag
     9901 aagatagcgg aattgtccga gaaaactggg caattcaacg agctgttgga tggcaagctg
     9961 gtttcggata tccgcatcga tgatcaggta tccacgattt tgaaggtcgt cagcaaaaaa
    10021 ctgcaaaaca cgagggacgg taaaacgttc ctgttgatga ccttgggcga ccgttccggg
    10081 acgatccgat ctatcgattg gaacaactcg aagtacaatg acgaacgttt gaccaacgga
    10141 acggtcgtgc gcgccactgg aaaggtctct cttttcgagg gacatccgca attgaacatc
    10201 gcgagctcga gagatgcgat caaggtgctc ggagaaggcg aattcgatcc caataaattc
    10261 atcgagatta cgtccaagaa tattcgagag atgtatgatt ctctactcaa acagatacag
    10321 aaggtcaaga acgataaagt gagggctttg cttcatgaag ccttcgtgaa gaacgaccga
    10381 ttcgttagcg atttcataga gtcgccggcc gcgatcaacg ttcaccacgc gtataaaggt
    10441 gggcttctcg agcacacgct ggatgtgacg gagctcgcca ttcaactaat ggaagcttac
    10501 ccggagatgc cgatcgacaa agatttggtc atcgcggggg cgctgcttca cgatatcggg
    10561 aaggtagagg agtattcgct ttctccgaca ggtatcgatc gcagcgacgc gggcgaactg
    10621 atcggccata ttgtcatcgg aatcaattgg ctcaccgaaa cggccgcgag gatcccccaa
    10681 tttccaaaaa aggcgctggt ggatttgcaa cacatcctct tgtcccatca cggagaaatc
    10741 gagtggggct cgcccgtgtt gccgaagacg atcgaagcgt tgatcgtgca ttcggctgac
    10801 aataccgatt ccaagatcgc ccaattcagg gagtatacgt tgaaggctca gcccaacatc
    10861 cttaacccgg atgataccgt ttggagcgat tttaataaat acctgtcccg caggattcgt
    10921 ataagaggag tggtatccta tggagaaaaa acgaaagatt aaattcggaa cgagcggatg
    10981 gcgagggatt ttggccgaag acttcacctt cgacaacgtt agaacggtct ctcaagcgat
    11041 cgctaccgag attttaaaag accccaaggc gaaaaacggc gcgttggtgg gttacgattc
    11101 tcggttcttg gccgaagact tcgcttgggc ggtcgctcaa gtgtttgccg gaaacggcat
    11161 ccgcacctac gtgtgtcatc aaccgacccc taccccggtg atctcctaca cgatccgaaa
    11221 cttgaacctg gccggcgggg tcaacatcac ggcctcgcac aacccccact tttggaacgg
    11281 catcaagttc aatcccgcga ccggcgggcc ggctttgcca gaagtcacca aagccatcga
    11341 aaccgagatc gaacgccttc agcggggcga aggagaggta cggttactca cccacctcga
    11401 tgaggagttc agtaagctcg tctattcgat cgacccaagt caggaatact tcgccgaaat
    11461 ccgacgaaag atcgatatgg accggatcaa aggggcggta aaaaaagtgg cggtggattt
    11521 ggtctacgga accggtgtcg attacttgga ccagctgctt ctctcgctcc ttccgggaga
    11581 agaggcgcag ggattggtcg tactccacga ttaccgggat ccctacttcg ggggataccg
    11641 cccggagccc gacaaaaaac ggctgagaga actcgggcaa gtggttttag aacaccgggc
    11701 gcagatcgga ctagccgtgg acggagatgc cgatcgattc gggatcgtcg atgacaaagg
    11761 tgtaccgatt acggccaacg aatacttggc gatcgtagcg tggcatctgt acaaaaacaa
    11821 gggattgcgg ggcgacagcg ctcgtagcgt ggcgaccagc cacgcgatgg atcgaatagt
    11881 cgagcacttc ggatttcaga ccctcgtgac ccccgtcggg ttcaagttca tcggcgacgc
    11941 gatcctcaat gaccgtatcg tgttgggcgg tgaggagagc ggcgggttga gcatacaaga
    12001 ccacgtgccg gaaaaggatg gaatcttggc ctgcctgttg gcactggaag ccatcgctta
    12061 tgagcagcgg ccgatgtcgg agatcagaaa aatcgtccaa gaggtgaccg gcccgtattt
    12121 cacagataga attgacctgg aattggaaag cgaaagtcgg aagcgcgagc ttatggactt
    12181 tttcgcaaat atgcaacgcg aggtttttgg gatacctgtg aaagaacgtc agaccgtcga
    12241 cggtataggc ttcgatctgt cgattccgga gggtgaagcc tggatcttga tccgggcctc
    12301 aggcaccgaa cctgtgatta ggatctacat cgaaagtacg caggagaaag tttttttgaa
    12361 acttcaggaa ggcgttgaaa agctcggatg aaaactctcg tcatcgtcga gtcgccctcg
    12421 aaagccaaaa cgatcgaaaa atacttagga aaagattatc acgttatctc ttccaaaggc
    12481 cacatacgcg atttacctcc ggacgaacta ggcgtcgata tcgaggcctc tttcgaaccg
    12541 aagttcacgc taatgcgtgg aaaagagaag gtcgtccagc agatcaaaga ggaagcgagg
    12601 aacaagcccg ttctgctcgc ttcggatatg gaccgggaag gggaggctat cgcttggcat
    12661 ctagcgcaga tcctatcgct gagcgatcaa gagaccaacc gcgttttgtt ttcagagatc
    12721 accgaaaaag ccattaaggc ggcgataaaa agacctttaa ggatcaaccg gtctaaggtg
    12781 gaggcgcagt tcgcgaggcg gatactggac cgactcgtcg gttaccagat cagccccttc
    12841 ttatggaagg tcatccaggt gaaatctctg agcgcaggac gcgttcaatc cgttgcgttg
    12901 cggtttattt gcgaccttga ggaaaagatc ttgacatttg ttcccgagga gtactggaag
    12961 ttcaaagcgc gcttcctgtg cgagaagatg caaggggaac tggaaaccgc gctggtgtca
    13021 gtagacggcg tgtccttgga aaagcagcct atcaggaacg tacaagacgc gatgaaaata
    13081 gaagaggaga tcttgaaatc ctccttcgcg gtgcgttcgg tcgaagaaaa agtgatcaac
    13141 cggaaacctc cggccccctt catcacgagc acgctacaac aagaagcgtc gaaccgcttg
    13201 ggcttttcgg tcagtcgaac gatgaaagtg gcgcaagagc tttatgaagg cttagagaca
    13261 aaggatgggc ctttggcctt catcacctat atgagaacgg actcgaatcg tatctcggaa
    13321 gaggccttgg aagcggcaaa actatacata accgagcatt ttggagaatc gcattcgaaa
    13381 acgcaaaggt atggaaagaa caaggcgaat acgcaggatg cccacgaagc gattcggccc
    13441 acctacccgg atgtcgatcc gcagaaagct gaaaaaatgc tttcggccga tcaggccaag
    13501 ctctaccggc ttatatggag ccgttttatg gcgtcgcaga tgataccggc agtatatagg
    13561 caaacaaaga taaagataga agatattgaa caagggtgct acacctttga agccgaaggc
    13621 gcgcgttgtg tggaggaagg atatgaaaaa gtatacccgc ccacctcgaa agaaaaagac
    13681 ctctctgtcg ttccggtagc gggtacgccg gcctctttgt cgcatttcga aaaagcccag
    13741 aactttacga caccacccgc acgattcacc gaagcgtcct tagtcaagaa gctcgaaaaa
    13801 gaagggatcg gtcggccgag cacctacgcc agcatcatcc acacactgct agaacgacat
    13861 tacgcctatc gcgaacagaa aagcctcgtc cccaccttcc tgggaatgat cgttaaccac
    13921 tttatgtccg attttttccc caagatcatc caagcgaagt tcacggcatc gatggaagag
    13981 aacctcgaca aagtcgaaga aggagaagag aattggaagg aagcgatcca acggttttac
    14041 tcggacttcg agcccacgct cttaaaacta aaggaaaaaa cccttacgaa gtccctcaat
    14101 tggttattct cgactaacct cccttgcaaa gaaaacgaaa aacatacgtt gatactgaag
    14161 atggggcgct tcggccccta tttacggtgt gaagcctgcg ataaaaacta ttcgatatcg
    14221 gaaaaggcga aaattatatt ccgacaaaac caagccgaga tatacggcga gaccgtactc
    14281 gagaatagca aagaggtggt cgcgatcggt agggcttgcc ccaaatgcgg gggcgacttg
    14341 gtcgaacgcc aaggcagata cgggaagttc atcgcgtgtt cgaactaccc caaatgcaaa
    14401 tacaccgaaa acgtggacga gaaggtagcg gcgaagtgcc cgaagtgtgg cgccgacgtc
    14461 gtgcgtaaga agagcaagcg agggagaacc tttttcgctt gcaaaaacaa cgcctataac
    14521 gggggcacgt gcgatttttt ctcttggtac gaaccgacgg aagagaagtg tccccagtgc
    14581 ggacaaacga tggttctgaa aaaggggagg tcaggagagg aaagcatcgt ctgtgagtct
    14641 tgtgcgcgta ccactcggat cgctaagaaa acgaaataag cggatttctt ttcctgatcc
    14701 ttctcctgtg gtataatgaa ctatcctctg attcaagggg gagaggggcg gccatctttc
    14761 cggggtgaaa acccttagaa agatggcttt tttttggtgg tgatcaaaat gactgacaaa
    14821 attgctatca caattgggga aaaagcgcta tcctattggc ttcgcattat gctggcccag
    14881 aaggtcgaga aaatccagga cgtggaagtc agttgtgagc tacaagggtt ccagcttgtt
    14941 gtgcgggtgg gcatacccgg attcaacgcc ggtgtccaag ccatcttccg cttacttcag
    15001 gagcttcggg atgagggggg tatccacact ctcctttttt cagtcaaacc cgtcggtttg
    15061 ttctcctcga tggcgctcgc tactttctca aaggctttcg atcggctgaa accgggtatc
    15121 gttttcgccg acaacactct cagggtatcc tttcgtgacc ttctcgcccc aaacggacca
    15181 ttacaggcgc cacttcttga gggttacagc atcgaatcga tcgacttctt gccatccacg
    15241 ttttccattg ttctaaaatt gaaagccact tcctagtgtt tgtcttccgg gcgaaagcct
    15301 ttgcgctttt gcctacgttt taccacgttt gggaacgcca aacttaagtt tggcacggtt
    15361 ttccgcgaca atagcaaaag accaagggcg gatctcgggg tcgcaaaaac cacttacccc
    15421 gcgataacct cacacgtcat tgaggcgtaa tcgttttttt ctttccgcga ctgctcgaaa
    15481 aaagggcatt cacatggagg gcacgtagga aaattttagt gaacgccctg ggaatgccct
    15541 gg
//
LOCUS       DAOPXN010000041        15530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3866_length_15530_cov_6.115024, whole genome shotgun sequence.
ACCESSION   DAOPXN010000041 DAOPXN010000000
VERSION     DAOPXN010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3866_length_15530_cov_6.115024"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            722..1915
                     /locus_tag="PKM49_03695"
     CDS             722..1915
                     /locus_tag="PKM49_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012955749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HNY81948.1"
                     /translation="MRTVGTVVRGIRTPIICTGDPLQRVVVDSLLLAAKEESFSFRDR
                     DIVAITEAVVAIAQGNYATVDQIAVDIRSKYPSGHIGVVFPTPLSRNRFAILLQGIAR
                     GAKKLTILYSYPADEVGNALFREDLLENFGINPCTDEFSGQEYEIRFGDEVHPITGIN
                     YPRYYREIVEKEGCEIDYLFSNRAASILDFCSDVLVANVHRREKTKRQLREAGANVVF
                     GTDDILTQSVLGSGYNPKYGLLGSNRATEEKVKLFPRDGERLVNGVQQELRERTSKEI
                     EVLVYGDGAFKDPVTGIWELADPVSCVSHTEGLGGTPNEVKLKYLVNSQFSSLQGEEL
                     NRAVKETIRQKDKTLTGKMWSQGTTPRRLTDLLSSLCDLTTGSGDKGTPVVLIQGYFD
                     NYSDA"
     gene            2689..3993
                     /locus_tag="PKM49_03700"
     CDS             2689..3993
                     /locus_tag="PKM49_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY81949.1"
                     /translation="MGEIYSRSNFVLFALGRLVSLIGAGIQMIALPLFILDTTGSASM
                     MGIFTLLSIVPRLAATPFAGVLGDRWNRKKIMVYADFGHGVILYLLGVIALYRQITIP
                     VLFVSTALLSVLDAFFSGATAGMLPDIVPEANLKRANSTLGLVSGISSIIGPVVGGIL
                     YGLTGILGVFFINATCFTLSAISEIFIRYEPKLRSEKLSVVGFVRDLKEGAPFVFAKK
                     GLKVLFIFAMFLNFLSAPLLYIVFPFILKTTIQFSSDQYGYLQSAFVVGALIGNIVII
                     KFLLKASNRKMLIGGLFAQYGFLFLFALTLFQPMFGWLQSRLSLYFWIGAAFFALTGY
                     FNTLVNVPINTNLQVMTPTHIRSRVFSVLDLLSQIMIPIGAVIYGYLMDNIPVHWFLI
                     ANNALCVLVTVVFLWVAPPEVYDPHLESATTVENAVSCSGNL"
     gene            complement(4016..5803)
                     /locus_tag="PKM49_03705"
     CDS             complement(4016..5803)
                     /locus_tag="PKM49_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY81950.1"
                     /translation="MLNGHRKEYLWVFVIVSLFCFVSCGNGTVQDVTLPMIRLLSPID
                     GARDLGTTVTLTWEATPSGAATPERPQALGTIAGYRVYFTVAGTNYPAPTFCQEKQLV
                     QTGLAEGTDYRWKVEVVEQAGKSRVSPEFRFSTKAGTTPQWGEETLSEPAVFTVEGAI
                     WVSPTGNDVTGIGSETRPYRTIFKALSECEGGETILLCSGEYREAVRIRWPNITLRSA
                     PGHWAKIIVPTDDEEEAIALMMDVESSGSKIQSLEIIGGYYYGIKFETRWDWGIEDRS
                     GASDIIIEDCVIHHTGRDAVKITPQCNRITIRRCEIYASGVGAHNQGNPNAEGIDNVN
                     GDRMLVQECHIHDVSTTGIYFKGGARECIVERNIIEHCGASGILIGFDTSPEYFDITT
                     NPEYYESISGIVRNNVILDTAYAGIGLYAAKDAQVYHNTIVRTAREGMSPITFGLSFQ
                     DWEPEAGRPPSINPIILNNLVYQESGERPIVAIRYSDELGGMSALEGPLVIDANCYYA
                     PGGEIWFQDRRPGHYLERGDFEDWKAHVGAEANSLCADPLLTERYRLEAGSPCIDKGI
                     PTDLVRYDVDNRLRPSPFDIGASERLATD"
     gene            complement(5876..10033)
                     /locus_tag="PKM49_03710"
     CDS             complement(5876..10033)
                     /locus_tag="PKM49_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY81951.1"
                     /translation="MHSVNYETLIRQAPFAYALYEVVFDENSKPSDFTFLETNESYEK
                     MTGLKLENIVGKKLTEVLSEIGSKGFDWVSVFSEVARNGSEKNFEFYSIPLARWYRVR
                     VSSLGNDLITTIFYDISSEKHRAEELERFFSVNLDLLCIADMDGHLLKVNQTWREILG
                     YTEEELEQNLFLDFVHPDDHLATIEAVEKLKNNQSVPNFVNRYRSKEGSYRYIEWRSV
                     SSGKLIYAAGRDITRRLLEQREAEASRDHLESILSHVPAIIYSLVEAGKIAKFTYISR
                     NVEKILGHTPEDFYRNLRFQSDHIHPEDRPKVKERLKQEPIHGSYELGEYRIQNKAGD
                     YQWIYDKQTITVQPNGIREIIGAWWDITELKKAELQLKEAQRQVREQEARYTSLFNQS
                     HDAVFLLDLLGKIVDSNKRAEELSGFTRAELKGLRFDKTCDVPGLSQQILKRLLAGEK
                     FPLYERSFVRKNGSTVPVELSTELALDENDNPSHIQCVIRDISERKEIERKLIESEDN
                     FRTFFETMEEMIFVADKKGRFLYTNGALKEKLGYTSEELMGMHVLDTFLAQNRKEAEG
                     IFAEMLAGKRTTCVLPLVRKDQSLIPVEARVWYGKWGGQECLFGVSKDLTQEQAALQL
                     FNKVFEKNPTMMALSSASENRFFEVNPAFVERTGYTREETLGKNPEELGLACEPEMNR
                     LVQDTISKDGSIHNMEIKLRTKNQEVLEGLLSGEAIEIQGQKMHLTVMTDITAQKKAE
                     ALALQASRAKSDFLANMSHEIRTPLNGVVGFAELLQKTPLNLSQQQYVENLVTSARSL
                     MEIINEILDFSKIEAGKLELEKTRTDLAQLVESTVDIVKLTAAKKGLELLLSIQPKMP
                     RYAVVDSVRLKQILVNILGNAVKFTQVGEVEMSLTFDWKEDGKAAFHFSIRDTGIGID
                     SDQKKRLFNAFTQADSSVTRKYGGTGLGLAISSLLAKKMGSEIRVESEKGKGSTFSFS
                     VEALCEPDAESPFAPETPIHRALIVDDNRRLCQILQEQLKAWQIDAEICEDGLSALKA
                     ISQSGPYDVLLIDSQMPYLDGLQTIRIIRDKYERTPDKNAIILLNAELNGPRIAEAQR
                     GFSLQGILDKPVKTTALRTVLENLYTRAPTSEVFQKTETEEFAITVERIPHILVVEDN
                     PLNMLLIRTILKEIVPNAILTEADNGLQAVEAYENAPFDLILMDVQMPEMSGLEAAER
                     IRKSETGTQRRVPIIALTARAIQGEEEKCLKAGMDDFLTKPVNQVALRNILVKHFSSI
                     LETPAKEHQPSEIKTEVHFDKESFLGQINHRQDVFQSIIRSVLLEFGGYLKEFGQMIA
                     QGDPSTIRQKAHALKGIALNMFFPKMSKYTEEVEKNSESLSRDELEDYYARMLAEWAL
                     IKKELGD"
     gene            complement(11029..15204)
                     /locus_tag="PKM49_03715"
     CDS             complement(11029..15204)
                     /locus_tag="PKM49_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY81952.1"
                     /translation="MYSVNYEMLIKKAPFAYALHRIVLDEQEKPCDYVFLEINEAFEN
                     MTGLKAENTVGKRVTTVLPGILSDQFDWIAFYGDIALNGGEKTFDQYSAPLNRWYRVM
                     VHSPETGLFSTIFSDISSEKAKAEELERFFSVNLDLLCIADTDGNFLKVNEAWHEILG
                     YSVEELEKSRFLDFVHPEDLLPTLEAMKQLDSQKKVLNFVNRYRSKDGSYRFIEWRSF
                     PAGKLIYAAARDITQRIREKQEIESSRDNLERILSHVPAVIYSLITTEDCSKFTYISK
                     NVEKILGHTPEDFYLDSKFQRNHIHPEDLPQVNALLKCEPTQGSYELGEYRIQNKAGD
                     YQWIYDKQTITIQPDGNREIIGAWWDITDLKKAESQLQEAEKQIRDKEARYTSLFNQS
                     HDAVFILDLKGNVIESNKRAEELLGFTKQEMKGLPLDVTSAAPERSYQVLEQILSGNV
                     VPLYERPFRRKDGTTVPVEISAELVRDGEGLPTHIQSVVRDISERKRIEKKLIESEDN
                     FRTFFETMEDMIFVADRQGRVFFTNGAVTRKLGYSLDELMGMHVLDVHPVDRRKEAAE
                     IYSDMFAGKLTTCPLPLGRKDGSEVPVETRVWFGKWDGTECIFGVSKDLSQEQEALRM
                     FNRIFEKNPTSMALSSTEDDRFIEVNQAFIDRTGYTRDEVIGKKAKDLCLTEYPETFE
                     RIVEGFAKDGFVHNLEIKFRTKHGEILDGLFSGESFEVLGKKINLTVLADITAQKNAE
                     ITALQASRAKSEFLANMSHEIRTPLNGVIGFGELLLKTQLNPSQRQYAENLTVAGRSL
                     LEIINQILDFSKIEAGKLELEKTPEDLIHLVQSTLDIVKLNAEKKNLELLLAIQPDIP
                     RYAIVDPLRLKQILVNLLGNAVKFTQSGEVELTLTCEIKEGNNGVFRFSIRDTGIGID
                     HEQQKKLFNAFTQADSSVTRKYGGTGLGLMISNLLAKKMGSEIHVESEKGKGSTFSFT
                     IEAVCELDDAPFKPDKPIRRALIVDDNHRQCQILREQLRAWQIDAEVCGDGFSALKLL
                     DRSEEPFDVLLLDSKMPYLDGIETIRIIRKKYDRDSHKNRIILLNADQSDVRIAEIQK
                     SYSIWGILNKPIKLSELRMVLENLYAPEPLANMPISADSTDTVLTVARPPRILIVEDI
                     AMNMLLLRTLLKEMVPNAILVEAENGIQAVEAYKNNVIDLVLMDVQMPEMSGLEATEI
                     IREQERVTGRHVPVIALTAGAIKGEEEKCLQAGMDGFLTKPINPVALRNVLANHFSTM
                     STETPLSSPEAGQVIGAHFDKDQFMQQYAHRRDIYHSIIRSTLLEFGGYVEQFGQVIP
                     KGDPALIRGKAHALKGVSLNMFFPVMAKYAEEVEKNSQSLTREELEDYYSRMVKEWEQ
                     IKTEIGEILGLNT"
BASE COUNT         3497 a         4138 c         3767 g         4128 t
ORIGIN      
        1 tcattcagtt tttcgctttt ctggtttttg taatgaccac gcacatgttg agttaagaaa
       61 tagagccgga atcgaccgcg cggtttttag tccatatagt catcgcggcg caaaaaagac
      121 aaaaggcgga tcgcaaaaag ggatcgcgag acttcgtggg cttccgaagt cagcccgctg
      181 gttaccccgc gatacccaca cacgggcatc gcggcgtgag cgcttcttct tttcgcgacg
      241 gcgcgatact ttgtgggctg ggaacgccaa cttttaagtt ggcatgtctt gtgaaaatgc
      301 tcacacggat accacagagg tcacaaagaa gagattcttg ggaacggctt gggaacacct
      361 tgggaacgcc aacttttaag ttggcatgtc ttgaaaaaag ctcacacgga taccacagag
      421 atcacaaaga agagatgtgt tttcgaaact caacacatgg tttatgtgtt ctctgcgtgc
      481 tctgcgtact ctgtgcgaaa ccatctttta cttttttgaa cctgccgaac taaagttcgg
      541 cgttcccagg gcggatcgca aaaagaggat cgtgggggcg taaaaaccac ttaccccgca
      601 ataacccaca cggtgatgcg agctttcaga aaattatgat acaattatcc ggcctttttc
      661 aggcttatga cgcgcacgtt ccctttgtct gatagaactt gcgctttcgg gaggcgaaca
      721 gatgagaacc gtcggaaccg tggtcagagg gattcgaacc ccgatcatct gcaccggaga
      781 ccctttgcag cgggttgttg tcgattcttt gctccttgcc gcgaaggaag aaagcttttc
      841 tttcagggat agggatatcg tggcgattac tgaggcggtc gtcgccattg ctcaagggaa
      901 ctacgccacg gtcgatcaga tcgccgtgga catccggagt aaatatccga gcggtcatat
      961 cggtgtcgta tttcctacgc cgttgagccg caatcgcttc gccatcctcc ttcaggggat
     1021 cgccagagga gcgaagaagc tgacgatcct atacagttat cccgcagacg aggttggaaa
     1081 cgcgctcttc cgtgaagacc ttctcgagaa cttcggtatc aacccctgca cagacgagtt
     1141 cagtgggcag gagtacgaaa ttcgttttgg cgacgaagtc catccgatca ccgggatcaa
     1201 ctaccctcgg tattacaggg aaatcgtcga aaaagagggt tgtgagattg actacctttt
     1261 ctccaaccgg gccgcctcga tcctcgattt ctgctctgat gttctcgtgg ccaatgtgca
     1321 tcggcgcgag aaaaccaagc ggcagctgcg ggaagccggc gcgaacgtcg ttttcggaac
     1381 agatgatatc ctgacacaaa gtgttttggg gagcggctat aacccgaagt acggccttct
     1441 aggttctaat cgagccacag aagagaaagt taagctcttc ccaagagacg gtgagcgctt
     1501 ggtcaacgga gttcaacagg agctacgcga aagaacgtcc aaagagatag aggtcttggt
     1561 ctacggtgac ggcgctttta aggatccagt tacgggaatc tgggaactcg cggatcccgt
     1621 ctcctgcgtg tcccacaccg aagggctcgg ggggactcca aacgaagtga agctcaaata
     1681 cctggtgaac agccagttct cctctctaca aggcgaagag ctcaaccggg cggtcaaaga
     1741 gaccatccgc caaaaagata agaccctgac ggggaaaatg tggtcccaag gcacgactcc
     1801 aaggcgcctc accgacctcc tcagctctct ctgcgacctc acgacgggaa gcggtgataa
     1861 aggcaccccc gtcgttttga tccaaggcta ttttgacaac tactccgacg cgtgaggggc
     1921 tcgcggaaag atttgccagg ctggtaacgc cccgggaacg ccgaacttgt gttcggtatg
     1981 tcttttctga aacacaaaag aagtatcgcg cagagaacac ggagtacacg gagagaacac
     2041 agagaaacac cgtgtgggtc aagctatggg gtgcaatcaa gaagcaaggc cagatacgat
     2101 cgcacgattt ttgtccacga agtcatcgcg gcgtaagcgc ttcttcttct cgcgacggcg
     2161 cgatacgctc tttagaaaga cgaagaaggg atcgcgagac ttcgtgggct tccgaagtca
     2221 gcccgctggt taccccgcga taaccccact ggcatcgcgg cgtaagcgct ttttcttttc
     2281 gcgacggcgc gatacattct ttagaaggac gaagaaggga tcgcgagact tcgtgggctt
     2341 ccgaagtcag cccgctggtt accccgcgat gaacctctta gttatgctgc tccataactt
     2401 gacctacacg aaacaccttg agaacgtcct gggaacgctg aacttgtgtt cggcaggttt
     2461 taaaagccag ggcttctcac ggagaaccca gaatagcgcc ccgatacccg ctgtatcccc
     2521 ttatcccacc cggcctttca ttttattact tctcacttcc aaaattatcg tttgtgagtt
     2581 gacttttcca attttttgga gtataatgat aaaaatactg gattgatacc caaataattg
     2641 gaattgcaat tcaatttatc ttgtacgata aggaaaggag cagtggttat gggggagatt
     2701 tacagcagaa gtaatttcgt gttattcgct ttaggaaggt tggtgtcttt gatcggcgcg
     2761 ggcatacaga tgatcgcctt gcccctgttc atcctggaca ccaccggctc ggcctcgatg
     2821 atgggaattt tcacgctcct cagcatcgtt ccgaggctgg cggctacccc atttgcgggg
     2881 gttctggggg atagatggaa ccgaaagaag attatggtct atgccgactt tggccacggg
     2941 gttatcttgt acttgctcgg cgtgatcgcg ctctaccgac agatcactat ccccgtcttg
     3001 ttcgtcagca ccgcgctctt gtcggtcctg gatgccttct tttctggggc gaccgccggc
     3061 atgctcccgg atatcgttcc agaggcgaat ttaaagagag cgaactccac cttaggattg
     3121 gtgagtggta tctccagtat catcggcccg gtcgttggcg ggattcttta cgggttgacc
     3181 ggtatcctcg gcgtcttctt cattaacgcc acgtgcttca ccctttcggc catcagcgag
     3241 atctttattc gctacgaacc gaaactcaga tcagagaaat tgtcggtggt cggcttcgtc
     3301 agggacctaa aggaaggggc acccttcgta ttcgccaaga aagggctgaa agttctcttt
     3361 atattcgcga tgttcctgaa cttcctctct gcgccgctac tttacattgt tttcccgttc
     3421 atcctgaaaa cgaccatcca gttctcttct gaccaatacg gatacctgca gtcggctttc
     3481 gtcgtggggg ccttgatcgg aaatatcgtc atcattaagt tcctgttgaa agcttcaaac
     3541 aggaagatgc tcattggcgg actcttcgct cagtacggtt ttctgttcct cttcgcgctc
     3601 actctcttcc agccgatgtt cggttggctt cagagccgtc tttctctgta cttctggatc
     3661 ggggcggcgt ttttcgcgct gaccggatac ttcaacacat tggtcaacgt tcccataaac
     3721 acgaaccttc aagtaatgac acccacgcac atccgatctc gcgtgttttc tgtcctggat
     3781 ttgttatctc agattatgat ccctattggc gcggtgatct acggttattt gatggacaac
     3841 atacccgttc actggttcct catcgccaac aacgcgctct gcgtcctggt gacggtcgtc
     3901 ttcctgtggg tcgctccgcc agaggtctac gatccccact tggaaagcgc cacgacagtc
     3961 gaaaacgccg tttcatgttc tggaaacctc taaatgagat agcggaaaat ttagatcaat
     4021 ccgttgcgag gcgctcgctc gccccgatat cgaaaggcga tgggcgtaat cggttatcca
     4081 catcgtatcg tacgaggtcc gtgggtattc ctttgtcgat acaaggactg cccgcctcca
     4141 aacgataccg ctcggtcaag aggggatcgg cgcataaaga attcgcctcc gcgccgacgt
     4201 gcgctttcca gtcttcgaaa tcgccgcgct cgaggtaatg gcccggacgc ctgtcttgaa
     4261 accagatctc tcctcccggt gcgtaataac agttggcgtc gatcacgagc ggcccctcca
     4321 aagcgctcat cccgccgagc tcatcgctat accgtatggc gacgatcggg cgctccccag
     4381 attcttgata cacgagattg ttgaggataa tcgggttgat cgagggcggg cgccccgctt
     4441 caggttccca atcctggaaa gaaagaccga aggtgatcgg gctcatccct tcgcgagcgg
     4501 tccgaacgat cgtattgtga tagacctgag cgtcttttgc cgcatacaag ccgattccag
     4561 cgtacgctgt gtcgaggatg acgttgtttc gaacgattcc ggaaatgctt tcatagtact
     4621 ctgggttcgt cgtgatatcg aaatactccg gactcgtgtc gaatccgatg agaatgcccg
     4681 acgcaccgca gtgttcgatg atgttccgtt cgacgatgca ctctcgagcg ccgcctttga
     4741 aatagatgcc tgtcgtagat acgtcgtgga tatggcattc ctggaccaac atccgatctc
     4801 cgttgacgtt gtcgattcct tcggcgttcg ggttgccctg gttatgggcc ccaacacccg
     4861 atgcgtagat ttcgcaacgc ctgatggtga tccgattgca ttgaggggtg atctttaccg
     4921 catcccgtcc cgtatggtga atgacgcagt cctcgatgat gatgtcgctg gcgccgcttc
     4981 tgtcctcgat cccccaatcc caacgcgttt cgaacttgat cccgtagtaa tagcccccga
     5041 tgatctctaa actctgtatc ttgctgccgc tggactccac atccatcatc agcgcgatgg
     5101 cctcctcttc gtcatcggtc ggaacgataa ttttcgccca atggcccggc gccgaccgaa
     5161 gcgtgatatt cggccaacgg atgcgaacgg cttcccgata ctcgcccgaa cacaagagga
     5221 tcgtctctcc accctcacac tcggataacg ccttgaagat cgtgcggtat gggcgagtct
     5281 cgctcccaat ccctgtaacg tcgttgcccg tcggtgaaac ccagatcgcc ccctctaccg
     5341 tgaaaacggc cggttcagac agggtctcct cgccccactg cggggtcgtt cccgctttgg
     5401 tcgaaaaacg gaattctggg ctcacacggc tctttccagc ttgctccacc acctcgactt
     5461 tccagcgata gtcggtccct tcggccaatc cggtttggac gagctgcttt tcttgacaga
     5521 aagtgggcgc tgggtaattc gttcccgcta ccgtgaagta aacccgataa ccggcgatcg
     5581 tgcctagagc ctgtggccgc tcaggagtgg ctgccccgct gggcgtcgcc tcccacgtga
     5641 gggtcacggt cgtgcccagg tcgcgcgccc catcgatagg agagagtagc cgaatcatcg
     5701 gcagcgtaac gtcctggaca gtcccgtttc cacaagatac aaaacagaat agacttacga
     5761 taacgaagac ccacaaatac tctttcctat ggccatttag catacagaac cctcctcaaa
     5821 cacgttgcgg tttgtcaacc tgtaggcgcg atcatttgat ttgacatcgc tagtgtcaat
     5881 cgccgagctc cttcttgatg agcgcccatt ccgccagcat tcgggcgtag tagtcctcca
     5941 gttcgtctcg cgagagggac tcgctgtttt tctctacctc ttctgtatat ttcgacatct
     6001 tcggaaagaa catattcagt gctatgcctt tgagcgcatg cgccttttgg cgaatcgtcg
     6061 aaggatcccc ttgggcgatc atctggccga attctttcag atacccgcca aactcgagca
     6121 agacgctgcg gatgatgctt tgaaagacat cttgtcgatg attgatctgc ccaaggaagc
     6181 tctctttgtc aaaatgaact tccgttttta tctccgatgg ctggtgttcc ttcgcaggtg
     6241 tttccaaaat ggaagaaaaa tgcttcacaa ggatatttcg caacgccacc tgattgaccg
     6301 gcttagttag aaaatcatcc attcccgctt tcagacactt ctcttcttct ccttgtattg
     6361 cccgcgctgt taaggcaatg atcggaacgc gccgctgggt gccggtctct gatttccgaa
     6421 tcctctcggc tgcctccagc ccgctcatct cgggcatctg cacatccatc aatataaggt
     6481 cgaaaggggc gttttcgtaa gcttcgaccg cttggagccc attgtcagct tcagtgagaa
     6541 tggcattcgg cacgatctct ttgagtatgg ttctgatgag cagcatattc aacgggttat
     6601 cttcgacgac taagatatga ggaatgcgtt cgacggttat cgcaaattct tctgtttcag
     6661 ttttttggaa gacttcagaa gtcggcgctc gggtatagag gttttccaac accgttcgta
     6721 acgccgtagt cttgaccggt ttgtccagga tcccctgcaa agaaaagcct cgttgtgcct
     6781 cggctatgcg gggaccattc agttcggcgt tcagaagaat gatcgcgttt ttgtccggtg
     6841 tgcgttcgta cttgtcgcgg atgatgcgta tcgtctgaag tccgtccaaa tagggcatct
     6901 gtgaatcgat cagaagaacg tcgtacggac cggattgact gatcgctttt agcgcgctga
     6961 gcccgtcttc acaaatctcc gcgtcgatct gccaagcctt caactgctcc tgaagtatct
     7021 gacaaagcct gcggttatcg tcgacgatta aggcgcgatg aatcggagtt tccggagcga
     7081 aaggcgattc ggcatccggt tcgcaaagcg cttcgacaga aaaagaaaac gtggaccctt
     7141 tccccttttc gctttccacc cgaatctcgc tccccatttt tttcgctaat aaactcgaga
     7201 tcgccaaccc caatccggta ccgccatatt tacgggttac cgagctgtcc gcttgagtaa
     7261 aggcgttgaa cagccttttc ttttggtcgg aatcgatgcc aatgccagta tctctaatcg
     7321 agaaatgaaa ggccgctttc ccgtcctctt tccaatcgaa ggtcagggac atttccacct
     7381 ctccgacctg tgtgaatttc acggcgttgc ccaatatatt gaccaagatt tgtttcaacc
     7441 ggacggagtc gacaacggcg tatctcggca tcttcggctg gatcgacaga aggagttcca
     7501 accctttctt tgcggcggtc agttttacga tatcgaccgt cgattccacc agttgcgcca
     7561 agtccgtccg ggttttttcc aactccagtt ttccggcctc aatcttcgaa aaatccagga
     7621 tttcgttgat gatttccatt agcgaacggg ccgaagtgac caagttttcc acatactgtt
     7681 gctgggaaag gttgagcggt gttttctgca gaagctccgc gaaaccgaca accccgttta
     7741 agggagtccg aatctcgtgg ctcatattcg ccaaaaaatc gctcttcgcg cgactggcct
     7801 gtaaggctag tgcctccgct tttttttggg ctgtgatgtc ggtcatcact gtgagatgca
     7861 tcttttgacc ctgtatctct atggcttctc ctgatagtag cccctccaac acctcttggt
     7921 tttttgtacg taacttgatc tccatattat gaatcgaccc gtctttcgaa atggtgtctt
     7981 ggaccaaacg gttcatttcc ggctcacagg caaggcccaa ctcttccgga ttcttcccta
     8041 acgtctcttc gcgggtatac cccgttcttt ctacgaatgc cgggttcacc tcaaagaagc
     8101 gattctctga cgcagaagaa agggccatca tcgtcgggtt tttctcgaat accttgttga
     8161 acaattgtag agccgcttgc tcctgagtaa ggtctttgga taccccaaag aggcactcct
     8221 gccctcccca cttcccgtac cacactctcg cttccactgg tataagcgat tggtcttttc
     8281 gcaccaaggg taaaacgcag gttgtccgtt ttcccgctaa catttcggcg aaaattccct
     8341 ccgcttcctt tcggttctga gccagaaacg tgtccaacac gtgcataccc atcagctctt
     8401 cagatgtgta gccgagtttt tctttgagcg ctccgttcgt gtaaagaaaa cgcccttttt
     8461 tatctgcgac gaagatcatc tcttccatcg tttcaaagaa ggttcggaaa ttatcctcac
     8521 tttcgatcag tttacgttct atctctttcc gttcggagat gtcccgaatg acacattgta
     8581 tatggctggg attgtcgttc tcatcaaggg cgagctccgt cgaaagctcc actggaacgg
     8641 tggagccatt ctttcttacg aacgacctct catacaatgg gaacttttct ccggccagca
     8701 gcctttttag gatctgttgg cttagccccg gaacgtcaca ggtcttatcg aaacgtagcc
     8761 ctttcaactc ggctctggtg aaacccgata gctcttctgc tcgcttattc gagtcgacga
     8821 tcttgcctag caaatccaag agaaataccg cgtcgtgaga ttgattgaac aacgaggtat
     8881 acctggcttc ttgctccctg acctgtcttt gggcttcctt aagctgtaac tccgcttttt
     8941 taagctctgt gatatcccac caagccccga tgatctctcg aataccgttc ggttggaccg
     9001 tgatcgtctg tttgtcatag atccactgat aatcaccggc tttattctgt atccgatact
     9061 ctcccaattc ataacttccg tgaatgggct cttgtttcag gcgctctttc actttcgggc
     9121 ggtcttcggg atggatgtga tcgctctgaa accgcaagtt cctataaaaa tcttcgggcg
     9181 tatgtcccag aatcttctca acatttcggc tgatatacgt gaatttggcg atcttaccag
     9241 cctcaaccaa ggagtagatg atcgccggta cgtgagatag gatgctctcg aggtgatctc
     9301 ggctcgcttc ggcttcccgc tgttcgagca accttcgggt gatatcgcgg cccgcggcgt
     9361 agatcaattt cccggaggaa acgctgcgcc attcgatgta ccggtaagag ccttctttag
     9421 atcgatagcg attgacgaag tttggaacgc tttgattgtt tttcaacttt tctacagctt
     9481 caattgtcgc aagatgatca tccgggtgca cgaaatctaa aaaaagattc tgctccaact
     9541 cttcttcggt gtagccaagg atttcgcgcc acgtctggtt tactttgagc aagtgaccgt
     9601 ccatatccgc gatacacagt aggtcgagat tgacggaaaa gaacctttcc aattcctccg
     9661 cgcgatgctt ttcagacgaa atatcataaa agatcgtcgt gatcaaatcg tttccgaggg
     9721 agctgacccg aacgcgatac cagcgagcca agggtatcga gtagaattca aagttttttt
     9781 cgctcccgtt tcgcgcgacc tccgagaaaa cacttaccca atcgaagccc ttcgatccta
     9841 tttcgctcag cacctcggta agcttttttc ccacgatgtt ttctagctta agtcccgtca
     9901 tcttttcata ggactcgttg gtttctagga aagtgaaatc gctgggtttc gaattctcgt
     9961 cgaacacgac ctcatagagc gcataagcga acggcgcttg gcgtatcaac gtttcatagt
    10021 taacggaatg catcctgttt ttcctccaga aagttacaga tacgatcttt gcccgaagac
    10081 gacggagccc aaatctctca ggcttttttt ttgatttttg attataccat tcgctgcggg
    10141 ttgttgaccg ttggagattg ggtcaagtta tggagtgaca taacgaagag gttcatcgcg
    10201 ggagtaacca gcgggacccc gcgatacacg cccttcgccc ttctcaaaaa cggatcgcgc
    10261 cgtcgcgaaa agaagaagcg cttacgccgc gatgccgcgc ttgggaacgc ctgggaacgc
    10321 caacttttaa gttggcatgt ctttgaggct cttcgacaat ttacaagacg tacttaatta
    10381 acgtcttcta tcgtaataat tctgtagacc cttagaccgt ttcgctaatc cgctctaatc
    10441 gcataatcgg ccatctcgcg gactcttttt attcctctta tgttactttg tatttcttgg
    10501 atgatcagtg ggcttcaagt aatgtgtcga agagccgtct ttgagctttc gcacttaaga
    10561 gcgcacaaag ttacaaaaaa gagccaaaca aaaacggatc gcgccgtcgc aaaaagaaaa
    10621 agcgcttacg ccgcgatgct cgcacgggtt atcgcgggga aggtgtactt cacgaccccg
    10681 cgatagcctt ttctccttct caaaagcaga aaggcctccc tcggacttgc aaaccagtca
    10741 tagacagaat cttatccgaa aacaagataa gcgaaagctc gaatcgtgcc gcttagagaa
    10801 ttttccgacg agcgggcgtg acatatctca gtcccgccac gacagtgctc acacagatct
    10861 aagaacgtcc gaagcgtaaa ccggggactc gttgcgatct ctttcaccga aagcaactct
    10921 ccgcgacgat ctccttttca ccggagtttt gaggtacccc gtcaaggcta tcacgaccag
    10981 taacaggccg atcaaaaccc gctcaacccg tgcgtccgct tgaaccgttc aagtgttgag
    11041 accaagaatt tctcctattt ccgtcttgat ctgctcccat tccttgacca tgcgcgaata
    11101 gtagtcctcc aattcttcgc gtgtcaggga ctgactgttt ttctccactt cctcagcata
    11161 cttcgccatc acggggaaaa acatgttcag cgagactcct tttagcgcat gagccttccc
    11221 ccggataagc gcgggatccc ctttcgggat gacctgcccg aattgctcca catatccgcc
    11281 aaactcgagc agcgtgctcc ggataatgct atgatagatg tcccgacgat gagcatactg
    11341 ttgcataaat tggtctttgt caaaatgcgc cccgatgacc tgacccgctt cgggtgaaga
    11401 tagaggagtt tccgtggaca tcgtggaaaa gtggttggcc agaacattcc gcagggccac
    11461 tggattgatc ggttttgtca gaaacccatc cattcctgct tgtaggcact tttcctcttc
    11521 acctttaatc gctccggccg tcagggcaat caccgggaca tgtcttcccg tgacacgttc
    11581 ttgttcccgg ataatttccg tggcttccaa tccgctcatt tccggcatct ggacatccat
    11641 caacaccaga tcgataacat tgtttttata ggcttcaacg gcttggatcc cgttttcagc
    11701 ttccacgaga atcgcattcg gcaccatttc cttgagaagg gttcgcaaca gcaacatatt
    11761 catggcgata tcttcgacaa tcaggattcg cggcgggcgg gcgactgtca agaccgtatc
    11821 ggtcgaatcc gcgcttatcg gcatattcgc caatggttct ggggcgtata agttttccag
    11881 aaccattctt aactccgaca gtttgatggg tttattcagg ataccccaga tggaatagga
    11941 tttttgaatc tccgctattc gcacatcact ctgatcagcg ttcaagagaa tgattcggtt
    12001 tttgtgagaa tcgcggtcat actttttacg gatgatacgg atcgtttcga tgccatccaa
    12061 gtagggcatc ttcgagtcga gcaaaaggac atcgaagggc tcttcggacc ggtcgagcaa
    12121 tttcaaagcg gagaatccat cgccgcagac ctccgcatcg atctgccacg cccgtaattg
    12181 ctcgcggagg atctgacatt gccggtggtt atcatcgacg atgagcgcgc gacggatggg
    12241 cttatccggt ttaaagggag cgtcgtcgag ttcgcaaact gcctcgatgg taaaagagaa
    12301 ggtggaccct ttcccctttt cgctttccac gtgaatttcg ctacccatct ttttcgccaa
    12361 cagattggag atcatcagcc ctaatcccgt gccgccatat tttcgggtta ccgaactatc
    12421 ggcttgcgtg aacgcgttaa acagtttctt ttgttgctcg tggtcgattc cgatgccggt
    12481 atcccgaatt gaaaaacgga aaaccccgtt gttgccttct ttgatttcac aggtcagagt
    12541 gagctccacc tctccgcttt gggtaaactt gacggcgttt cctaataaat tgaccagaat
    12601 ctgcttgagg cgcaaaggat cgacgatggc atatctcggg atgtccggtt gaattgccag
    12661 aaggagttcc aaattctttt tttccgcgtt cagtttcaca atatccaggg tcgattgaac
    12721 cagatggatc aaatcctctg gagttttctc caattccaat tttccggcct ctatttttga
    12781 gaaatcaagg atttggttga tgatttccaa cagcgaacgc cccgcgaccg tcaggttttc
    12841 cgcatattga cgttgggaag gattcagttg ggttttcagt aacagttcgc cgaagccgat
    12901 gactccgttc agaggagtcc ggatttcatg actcatattc gctaaaaact cactcttggc
    12961 ccggctcgcc tgcaaggccg taatctccgc gttcttctgc gccgtgatgt cagccaggac
    13021 agtcaggtta atctttttgc ccagtacctc gaaagactcc ccggagaaaa gcccgtccag
    13081 tatctcacca tgtttcgtgc gaaacttgat ctccagatta tggacaaagc cgtctttcgc
    13141 gaatccctct acaattcgtt cgaatgtttc cgggtattcg gtcagacaca ggtctttggc
    13201 cttttttccg atcacttcat cacgagtata tcccgttcta tcgatgaacg cctgattgac
    13261 ttcgataaat cgatcatctt ccgttgaaga gagggccatc gaggtggggt ttttttcgaa
    13321 gattctattg aacattcgca gcgcttcctg ttcctggctc aggtctttgg aaactccgaa
    13381 aatgcattcc gttccgtccc acttcccgaa ccaaaccctc gtttcgaccg gtacttcgga
    13441 accgtctttt cggcccaggg gcagaggaca ggtcgtgagt ttaccagcaa acatatcaga
    13501 atagatttct gccgcttcct tccttcgatc tacaggatga acatcgagca cgtgcatgcc
    13561 catcagctcg tctagcgaat agccgagctt tcttgtgacg gctccattcg tgaaaaatac
    13621 tcgcccttgt ctgtccgcga cgaagatcat atcctccata gtctcgaaga atgttcgaaa
    13681 attgtcctcg ctttcgatta gttttttttc gatccttttc cgttcggaga tatctctgac
    13741 gacgctctgg atatgggtag gaagaccttc cccatcccga acaagctcgg cggatatctc
    13801 gaccggaacg gtggtaccgt cttttcgccg gaaaggccgt tcatagagcg gtactacgtt
    13861 accggaaagg atctgttcca atacttgata actgcgctcc ggggcggcgg aagttacatc
    13921 cagcggcaac cctttcattt cttgttttgt gaaacccagc aactcttcgg cacgtttatt
    13981 ggattcgatg acgtttccct ttaagtccag gatgaaaacg gcatcgtggg attgattgaa
    14041 gagcgaggtg taccgcgctt ccttgtcccg tatctgtttt tccgcttctt gtagttgaga
    14101 ttctgccttt ttaagatcgg tgatgtccca ccatgcgccg atgatctccc ggtttccgtc
    14161 gggttggatt gtgatcgtct gtttgtcata gatccactga taatcaccgg ctttattctg
    14221 tatccgatac tctcccaact cataactacc ctgagtcggc tcgcatttga ggagtgcatt
    14281 cacctgcggc aggtcttcag gatggatgtg attcctctga aacttactgt cgaggtaaaa
    14341 atcctccggg gtatgaccca ggatcttttc gacgttctta ctgatatagg tgaacttcga
    14401 gcaatcttca gtcgttatta aggaatagat gaccgccggt acatgagaca gaatacgttc
    14461 caggttgtct cgactggact cgatttcctg cttttctcgt attctttgag ttatatcgcg
    14521 ggcggccgca tagatcagtt tgccggcggg aaaggatcgc cactcgatga atcgatagga
    14581 accgtctttc gaccgatacc ggttgacgaa gtttagtacc tttttctgac tgtccagttg
    14641 tttcatcgct tcaagagtgg gaagcaagtc ctccggatgc acgaagtcca gaaatcggct
    14701 cttttccagt tcttcaactg agtaaccgag gatttcgtgc cacgcttcgt ttactttaag
    14761 gaaattaccg tccgtatcag cgatacacag caggtccaag ttaaccgaga agaatctttc
    14821 caactcttcc gctttggctt tttcggaaga gatgtcggaa aagatcgtcg aaaacaatcc
    14881 ggtctctggg gagtggacca ttacacgata ccagcggttc aaaggagcgg agtattgatc
    14941 gaaggttttt tcgccgccgt taagggcgat atctccgtaa aaggcgatcc aatcgaactg
    15001 atccgacaga atccctggca ataccgtggt tacccgtttt ccaaccgtgt tttccgcctt
    15061 gaggccggtc atattttcga aggcttcgtt aatctccaaa aagacataat cgcaaggctt
    15121 ctcctgttcg tcaaggacaa tccgatggag cgcgtaggca aaaggcgctt tctttatcaa
    15181 catttcatag ttaacggaat acatcccttt ttcctccaga gatttaccga tacgatcttt
    15241 gcccgaaaac gaggggggcc caatctcccg gtttttttcg gttttttgat tataccattc
    15301 tcgttgcgga ttgttgactg atgagtgtag gtaaagctat ggggtgacat aacaaagagg
    15361 gttatcgcgg ggtaaccaac gggctgactt cttgcgtgtt atcgcgtggt aaccaacagg
    15421 acaccgcgat actaactaag ccccgcgatc agtccgtata aaagcgtatc gcgccaaggg
    15481 gtaatcgcgg ggtaaccgaa gggaccccgc gatccccctt ttgttttttt
//
LOCUS       DAOPXN010000042        15399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3909_length_15399_cov_5.018379, whole genome shotgun sequence.
ACCESSION   DAOPXN010000042 DAOPXN010000000
VERSION     DAOPXN010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3909_length_15399_cov_5.018379"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2322)
                     /locus_tag="PKM49_03720"
     CDS             complement(43..2322)
                     /locus_tag="PKM49_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNY81953.1"
                     /translation="MRILPKLELFILSACMTLTLCFNLFGHWQEKRILLTSFYDSSET
                     EILHFEKVMDAFFIEAKANVLFLEELLAEVPLDDTLFSLVGLTGEGKVLPSYTSETDL
                     RLLSLFDGFLKTHRQFLMAYFGSANGGYIEVPVIELGKGYDPRVRPWFQKALQNPGQV
                     NITEVYQTLFSEVPQLGIVKAVSRGGTLCGVLGLSLSLKALTDMIRQGKIQRIGNVFL
                     VDASDRVLANSMDPMYNFGPLSALSSELPEGSIGRTWRLNINGKRTLGVSARSQILPW
                     RYIVFVDEDELFEPLKLNAFWFSLITLGILFFVVFFTIPVSRGFSRPIEELSRQMVRF
                     GKQHFFEQIHVKGFREIEEMSDSFNEMTGELKTLIEKNKAYYDELERINLALKRRNIE
                     LENTSEHLFDYSSRMEEMISITSRMCSSTIEKDESFLKDALEMILDLVPKADYGSVSL
                     VEDDKWRFVHTVGHDIETLKQLPLTVDMLEELQNAKSLERLMEADDGVFPPDLSDAFR
                     RATRSIRASILIPLKFSGQTLGTLSIEIAKESDEQFTSSDYKILQTFSNLASAFLKIQ
                     KYIQQQEGFQKYLITSMIRLLEIHDPYTKGHSENVANLSITIAEAMGLDKTLVSRVFW
                     SGMVHDIGKVLVPGVILSKPSMLTLKEYNLIKEHPVWGARVLEPFEDLKDVAKYVRHH
                     HERWDGLGYPEGIGGETIPLVSRILSVADALEAMTSDRPYRKRVSLTAALREIENNAG
                     SQFDPKIAEIAVRSIKKLA"
     gene            complement(2423..3361)
                     /gene="argF"
                     /locus_tag="PKM49_03725"
     CDS             complement(2423..3361)
                     /gene="argF"
                     /locus_tag="PKM49_03725"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNY81954.1"
                     /translation="MAVNLKGKHLIGIKHLTPEEFVQYVQMSEALKRDYLAGYRNELL
                     KNRTLAQIFQKPSLRTRVSFESAMTHLGGHGIYLGPDDIRLGQRETTEDIAKVLSGLC
                     DVIMARVFKHEAILDLAKYATVPIINGLCDTEHPTQIIGDFLTIWEKFRKFEGLKMTY
                     IGDGNNVCNSLIYGSALVGTRFTAICTPGYEPKPEVIRDANAIIAKYNTGSVIEVSNH
                     LDAVAGSDIIYTDVWASMGQESEAEERKKIFMSYQITMPVFKKAKPTAIFMHCLPAHY
                     GEEVVYEVAHCERSVIFDQAENRLHSIKAILVLLTP"
     gene            complement(3361..4320)
                     /locus_tag="PKM49_03730"
     CDS             complement(3361..4320)
                     /locus_tag="PKM49_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY81955.1"
                     /translation="MNAIGVDLGGTNIKAALVSQDGKFLAKKHVKTEVSSGKERVAAK
                     IADIVNDLGQGDRSICVGIGSPGSIDRKNGLVRFSPNLPGWLDFPLAPLVSEKTGREV
                     LIENDAKAAALGEKWFGEARGCDSFIVLTLGTGVGGGAVTNGQLLIGGGGIGGELGHM
                     CVDPRGPLCGCGSHGCLECYASSSGMRRLAEEYRARYPHSLVFQEQSGGQFEAIAAFS
                     AYQRGDPLAEIIYERFVFAMGVAIGSLVNIFNPEKIILSGGISSSAPLYLEKVKKISN
                     EYTVLSMMNSFDIVQSSLKENAAIYGAASIVFDRKLPHTSEVL"
     gene            complement(4339..4890)
                     /locus_tag="PKM49_03735"
     CDS             complement(4339..4890)
                     /locus_tag="PKM49_03735"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNY81956.1"
                     /translation="MIALTITGGEFKGRRIKALANEVTRYTSEKVRLACFSILKGHRD
                     IATSHFADLCAGSGVVGIEALSRGAPFVEFYDLSRESEKAIATSLRELGVQDRARVYR
                     RNLLISSRKLRLESEVVFIDPPFHDDIAQKFFQTFADSPNLMIKNALLMIEYRKSIPD
                     ITGFERLHTYRYGTVFLNLFRKE"
     gene            complement(4914..5807)
                     /locus_tag="PKM49_03740"
     CDS             complement(4914..5807)
                     /locus_tag="PKM49_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81957.1"
                     /translation="MGSLIKLGPPEILKAFEVFYVKALSNIDFHSIYQKIGKRGSEDY
                     LGFVVSAPAYPPLYILSQRQGPIVIGLGSNVRELIRKYGSAEMSTPPFFFDLYRELFR
                     AYTDTLPEKGQVKCFYSILGGEMVNVPSFAFLGQEKTSKTLLMRESVQGHEKMLVAGF
                     FSEEAMGNLLGAYEKWHGKGYRFDFEEDLASLSLKFPKEATPKYALSEYRLISKLIKQ
                     LLIEWFPHLFEPGEIGLVFPDNTYTLMSIRLLFKNLPKLMVSLSPFLAQFHSVLDLCA
                     KTLSEQLHAGLRFFERTDTLP"
     gene            complement(5817..6866)
                     /gene="pseI"
                     /locus_tag="PKM49_03745"
     CDS             complement(5817..6866)
                     /gene="pseI"
                     /locus_tag="PKM49_03745"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015054269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HNY81958.1"
                     /translation="MQFRIGERLIGDHAPVFIVAEMSANHQQDFDVAVKIIKAAKKTG
                     ADAIKMQTYTPDSLTIDCDNEYFSLKQGTIWDGQTLYRLYQKAYTPWEWQPKLKQIAE
                     EEGMICFSTPFDTKAVDFLEGMNVPAYKIASFEITDIPLIEYIATKKKPVLFSTGVAT
                     ISDIDLALKTLRNKGIADIVLLKCTSEYPAPLEAANLRTIPNMAETFRVVPGLSDHTL
                     GITVPLTAVALGAKVIEKHFTLDRSAGGADAAFSLEPEEFTQMVQGIRECEKALGEVN
                     YTVAESTLKKGRVFSRSLFVIKDMEAGELFTSENVRSIRPGYGLHPVFLRDILGKKAR
                     VDIRFGTPLHWDLVD"
     gene            7100..8251
                     /locus_tag="PKM49_03750"
     CDS             7100..8251
                     /locus_tag="PKM49_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNY81959.1"
                     /translation="MHKTFLIVASVEAHIFGFHLPVLTYFQERGFEVHIATRFSKRLG
                     FFESQGYICHDIPFSRSPFAFDNGAALRALTKLFRARRFDAIHTHTPAASFLCRWAAQ
                     RTRQPYVAYTAHGLHVYRGAPISHRLLYGTAERLAARWTDTLITMNREDTDWAKENLR
                     LKKGGSTVYIPGVGIDLERYQMAGFDRAEFRKSLDVFEDAFILSFIGELNKNKNQLML
                     IEACERFKPSEPIRLLLVGEGPMEETYRRYVQERGLNNIHFLGFRDDIPKILAVSDVI
                     VLTSKREGLPRCVMEGMAAGKPILGTNIRGTRDLVIDGENGFLVEVGDVGALTEKIRE
                     LYTNAPLRLQMGERNLKRIKDFALPKVMDALDKVYIDAGLFKTEEERGR"
     gene            complement(8248..9078)
                     /locus_tag="PKM49_03755"
     CDS             complement(8248..9078)
                     /locus_tag="PKM49_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNY81960.1"
                     /translation="MKRAFVMGILLAIVWLLSGCNLITVKESFSKDIALGSIKSLVVQ
                     TKNGAIEFLGTEGSSISVRGEKRVQGLGNLDEEIKKIIISYDLQGDRLIIKSESPSDG
                     NFFTKASYGTKFVVYLPPNALASITGRTSNGAVKAQNLETSIQLTTTNGGVDLRNTRG
                     RIQADTTNGGIDLYRVELPGGNNVFSTSNGTIQGDLAFAGNGDCKISTSNGRISLRVP
                     SRSAFDFRASTSNGSIDFFGLSVLLTQSEKNLKSGRVNTGGFRLDLATSNGTIRVEGF
                     "
     gene            complement(9106..9294)
                     /locus_tag="PKM49_03760"
     CDS             complement(9106..9294)
                     /locus_tag="PKM49_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNY81961.1"
                     /translation="MADYKRLYRSRTDRRLAGVCGGIGNYFEVDPNPVRVVWAVTCFI
                     GLGILMYLLAWIIIPEEP"
     gene            complement(9392..11152)
                     /gene="uvrC"
                     /locus_tag="PKM49_03765"
     CDS             complement(9392..11152)
                     /gene="uvrC"
                     /locus_tag="PKM49_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNY81962.1"
                     /translation="MVVDLEPNEFLEHPLKEVALRFPDEPGVYVFSQQDKPIYIGKAK
                     SLKKRILSYFRQTNGERHKVSGILQRSDQLKYILVSSEKEAFMLEANLIYENKPAFNT
                     MLKDNRFYPYVLITQEKFPRIKVVRDKVEKGKYYGPFSSVGMVRQVFEIIYRTYGIRP
                     CDYDLSKVRKPCLEYQLGRCSAPCAFISEEDYNEQLKKVERFLKGNIHSLKTLMKEKM
                     AFYSGHLLYEKAKIVRDLLTKLDTLFFPQYITLPKNINQDFFAIDLAEGKTVVFRMKD
                     GAVFGCLTLDIDGVLEVEEILSQFYYGRHSEIPDSIILNPGAIPEERIKGLFDVSYIG
                     PPRTVGEEKILEIASNNLIKEVEKKRLSVESLKLLQVQLGLKKYPATIEGIDIAHTQG
                     LYTVASVVSFSNGFPDKSRYRKYRISQLTQPDDFEALRIVTRRRYSKHPLPELLLIDG
                     GIGQVGAVKEVLEKELHITDYQMIGLAKEEETIVFPDDRVALKLPENSPSLRVLVFVR
                     DESHRFANTFHAQLRDDRMEKTALEDIPGVGKVRKIKLLKRFHSLKNISKAEVSEITA
                     VVRDQALAIRIKEYLQRNFN"
     gene            complement(11160..12467)
                     /locus_tag="PKM49_03770"
     CDS             complement(11160..12467)
                     /locus_tag="PKM49_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNY81963.1"
                     /translation="MSQKPLWEQGEDVPLAHRIRPRTLDEVLGQDHLFSQGSVLRKAI
                     ENDQLFSAIFYGPPGCGKTTVGGIIKERTRHPFAHFSAARSTMSQLKPILDEASKRFA
                     LSKQSTILFVDEIHRFNRLQQDVLLPYIENGEVVFIGATTENPSFEINPALLSRCKVF
                     TFQELSKQAIAKIFLNGKAALDPNNEITVEEDALNVLVDWSSGDARTILNYAEVLFFG
                     MRQSGIAVLSHAVIERFLQKSTAKYRKHGDEHFDLISAFIKSMRGSDPDAAVYYLTRM
                     LESGEDPLYVARRIVRFASEDIGLAEPQAMSVAVSCFQAAHYIGLPECTTSLAQAAVY
                     MAIAPKSNRIYVAYESAKKEVARNPDLPVPFRLRNPVTPFMKESGYGKDYLYPHDSDT
                     GFIREPYLPESLQEQRFYVPSFIGKEKKIREYLESIWKRTYPK"
     gene            complement(12477..13409)
                     /locus_tag="PKM49_03775"
     CDS             complement(12477..13409)
                     /locus_tag="PKM49_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY81964.1"
                     /translation="MKRALVTGGAGFIGSHLVDALIEEEYDVAVIDDLSTGSLDNLPP
                     KGIRLFKGSVSDAELVREAFSSHKPDYVFHLAAQISVSRSVREPAFDADINVVGSIRI
                     MEACVREAVKKIIYTSSGGVMYGENPPVLPTPETSPCAPFSPYGIAKLTGEKYLAFFE
                     KEHGLRWTSLRYANVYGPRQNPHGEAGVVAIFLQKMLRGQPVTINGDGEYIRDYVYVQ
                     DIVRANLLAIDRADGLALNIGTGSGLSVNRLFGLLRDQTHYRQEAFHGPARPGDLRKS
                     VLDSSLAKTTLGWSPLMGLTEGISETVRYFEKTT"
     gene            complement(13419..13874)
                     /locus_tag="PKM49_03780"
     CDS             complement(13419..13874)
                     /locus_tag="PKM49_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNY81965.1"
                     /translation="MTRGRKKVLTSALEDYIKVIFDLTQAAPVARVKDIAESVGVSLP
                     SVTNAMKRLKDLGYVNYEKYGLIYLSEEGKKRAITLIRVHQLLDAFFVDLVGMPANVS
                     SNLSCRIEHFLDQRTEERSARFITILKKIKTGEPEAYNDLERFLQEKPG"
     gene            complement(13891..14781)
                     /locus_tag="PKM49_03785"
     CDS             complement(13891..14781)
                     /locus_tag="PKM49_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81966.1"
                     /translation="MAYIPGPGTKEKKPATSVESVPVSIEEKPLKRYYEERARLRKKE
                     KSSRKTPLKEGETSETPVPEPKTRTPATRESDGPKRATITGTKRLVTKQPPTFVWTFL
                     FFVALAFSIFSYMKLHEFRNTYATQLDALKQKSEERFQTLNKMIMEDRAYLGPNGIID
                     KYLVVSNNIDSQIIGLNDMIALSESRAFDKVGFVRFFISGSKPVWVSVESSSGKVVFQ
                     NTLSPGLSREMIYYFKKPAQAIQNITFEVTQNFKVSSGNYENTYLVFFSFGTTKIVRM
                     DSKTISNPVQKYNIWIPSTT"
     gene            complement(14781..>15399)
                     /locus_tag="PKM49_03790"
     CDS             complement(14781..>15399)
                     /locus_tag="PKM49_03790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY81967.1"
                     /translation="LVLYAQDGVEEAFSLLVERYENMVLKFANQYYGQWAEKSDIIQS
                     GYVGLIKAIYNYRTDKDANFNTFAWTNIHSEIKTFLTFLNRKKNKILTESYSFDYVSS
                     EEAEEEEPMMTKVSTQESEAINLKDLLLEKTTERMKASLSEEEMSIFVMYTESYTYSE
                     IARRYGINIKKVDNIIQKCRKSFKAFYEEEQGILDKSEEILRRAV"
BASE COUNT         3560 a         4131 c         3705 g         4003 t
ORIGIN      
        1 gtcggtggat ttgatcgaga gaaatatcct ttctctttgt atctaagcaa gttttttaat
       61 cgatcttact gcgatttcag cgatcttcgg atcgaattgg gatcccgcat tgttctcgat
      121 ctccctcaac gcggcagtta acgatactct ttttcggtaa ggccgatcgc tcgtcatagc
      181 ttccaacgcg tctgccacgg acagtatccg cgatacgagt ggaatggttt ctcccccgat
      241 cccttcgggg taacccagcc catcccaccg ttcgtggtga tgtcgcacat acttcgcgac
      301 gtctttgagg tcttcgaagg gctccaacac ccgggccccc cacacgggat gctctttgat
      361 gaggttgtac tctttcaacg tcagcataga gggtttggac aggatgactc ccggaaccag
      421 caccttcccg atatcgtgca ccattccgga ccaaaaaacg cgcgagacga gcgttttatc
      481 taaacccatc gcttccgcga tcgtgataga gaggttcgcc acattctccg aatgcccctt
      541 cgtatatggg tcgtggattt ccagcaaacg gatcatagag gtgatcagat atttctggaa
      601 accttcttgc tgttggatgt acttctgaat cttcagaaaa gccgacgcca ggttcgagaa
      661 ggtctgtagg attttgtagt ctgagctcgt aaactgctca tcgctttcct tcgcgatctc
      721 gatggacaga gtccccaagg tttgcccgct gaacttcagg ggtatcagaa tagaggctct
      781 aatcgatcgg gtcgcacgac gaaaagcgtc gctcaaatca ggtggaaaga ccccatcgtc
      841 ggcttccatc aagcgctcga gcgatttcgc gttttgtagt tcctcgagca tatcgacggt
      901 gagcggaagc tgtttcaggg tctcgatgtc gtggccaacc gtatgcacaa aacgccattt
      961 atcgtcttcc accagagaga cgctcccgta atcggccttc gggaccaggt ccaaaatcat
     1021 ctctaacgcg tccttaagga agctttcgtc cttttcgatc gtcgacgagc acatccgaga
     1081 agtgatcgaa atcatctctt ccatcctgct cgaatagtca aaaaggtgct cgctcgtgtt
     1141 ttccagttcg atgtttctcc gtttaagcgc caggtttatg cgctcgagtt cgtcgtaata
     1201 ggctttgttc ttttctatca gtgttttaag ctccccggtc atctcattga aggagtcgga
     1261 catctcttcg atttctcgga agcccttcac gtggatttgt tcaaaaaagt gttgttttcc
     1321 gaaccgaacc atctgtctgg atagctcttc gatcgggcga gagaacccgc gactgacagg
     1381 aatcgtaaag aacaccacaa aaaataaaat acctagcgtg atgagcgaga accagaaggc
     1441 attaagtttc agaggttcga acagttcgtc ctcatcgacg aaaacaatgt aacgccaggg
     1501 gagaatctgg cttcgggcag aaacgccgag ggttctcttg ccgtttatgt tcagacgcca
     1561 cgtccttcct atggaccctt cgggcaactc ggacgacaag gctgacaaag gaccgaagtt
     1621 atacataggg tccatagagt tcgccaatac cctatcggaa gcatcgacca aaaacacgtt
     1681 tccgatcctt tggattttac cctggcgaat catatccgtc agggccttca gagaaaggct
     1741 taatccaagg acgccgcaca gagtacctcc gcgtgagacc gctttcacta tgcccaactg
     1801 cggaacctcg gagaagaggg tttgatacac ttctgtgatg ttcacctggc ccgggttctg
     1861 taaggctttc tgaaaccaag ggcgaactct cgggtcatag cctttaccca gttcgattac
     1921 cgggacctcg atataccccc cgttggccga accgaaatag gccatcaaga actggcgatg
     1981 cgttttaaga aatccgtcga agagagataa caaccgtaga tccgtttccg aggtatagga
     2041 agggagcacc ttgccttctc cggtcaatcc taccaaagag aagagggtat cgtctaaagg
     2101 aacctccgcg agcagttcct cgagaaacaa cacgttggct ttcgcctcta taaaaaacgc
     2161 gtccatcacc ttttcaaaat gcaagatttc cgtttccgag gaatcgtaga aagatgtgag
     2221 caagatgcgt ttttcctgcc aatgtccaaa caggttaaaa catagggtca gggtcatgca
     2281 cgcagataag atgaagagct ccaatttcgg cagtatcctc atcgaaaacg ctcccttttt
     2341 agaaagtcga gattacgaga ccgcgagcga ctctattaag gaggaggctt ccgggtgtcg
     2401 acgcagaaac ctcctggtga atttatggcg tcaagagcac caaaatggcc tttatcgagt
     2461 gcaatctgtt ttcggcttgg tcgaagatga cgctcctctc acagtgcgct acctcgtaga
     2521 cgacttcctc tccgtaatgc gcgggcaggc agtgcatgaa gatcgccgtt ggcttcgcct
     2581 tcttaaagac cggcatcgtg atctggtatg acataaatat ctttttccgt tcttcggctt
     2641 ctgattcctg gcccatactg gcccaaacgt ccgtgtaaat gatatccgag cccgccacag
     2701 catcgagatg gttcgacact tctatgacgc taccggtatt gtatttcgcg atgatggcgt
     2761 tcgcgtctcg gatcacttcc ggcttgggct cgtatccagg cgtacagatg gcggtgaaac
     2821 gcgttccaac caatgcagac ccatagatca gagaattgca gacgttgttt ccgtctccga
     2881 tataggtcat tttcaaacct tcgaacttcc ggaacttctc ccagatggtt agaaagtcac
     2941 cgataatctg agtgggatgt tcggtgtcgc aaaggccatt aattatcgga acggtcgcgt
     3001 acttggccag atccaatatc gcctcgtgtt tgaacactct ggccataatc acgtcgcaaa
     3061 ggccggacaa taccttcgcg atgtcttcgg tcgtctcgcg ttgtcccagt ctgatatcat
     3121 ccggtcccag atagatcccg tgtcccccga gatgggtcat cgcggattca aaagaaaccc
     3181 gagttcgtaa agaaggtttt tggaagatct gcgccaaggt tctgtttttc aacaactcgt
     3241 ttctgtagcc ggccaaataa tctctcttca acgcctcgga catctgaacg tactgcacaa
     3301 attcttcggg cgtcaagtgc ttgatcccga tcaaatgctt tcctttcaag ttcactgcca
     3361 ttagagcacc tccgatgtat gtggtagttt cctgtcaaaa acgatcgatg cggcgccgta
     3421 gatcgctgcg ttctctttta gcgaactttg cacgatatcg aaagaattca tcatcgatag
     3481 aaccgtgtac tcgttggaga tctttttcac cttttctaaa tacaaaggcg cggaagaaga
     3541 gatccccccg cttaaaatga tcttttcggg gttgaagatg ttcaccaaag agccgatcgc
     3601 gacgcccatc gcgaacacga accgctcgta tatgatctcc gctaacgggt ctcccctctg
     3661 gtaagcggag aacgcggcta tcgcttcaaa ttgcccaccc gattgttctt ggaatactaa
     3721 agagtgtggg tatcgcgcgc gatactcttc cgccaacctg cgcatcccgg agctactcgc
     3781 gtagcattcc aaacacccgt ggctcccgca tccgcaaagc gggcctcgag gatccacgca
     3841 catatggccc agctccccgc cgattccgcc tccgcctatg agaagttgac cgttcgtcac
     3901 ggcccctccg cccacaccgg tccctaaggt cagcacgatg aagctatcgc acccgcgcgc
     3961 ttctccgaac catttttctc ccagcgcggc ggccttcgcg tcgttttcga tcaaaacctc
     4021 gcgccccgtt ttttctgaaa ccagcggcgc aaggggaaaa tccagccaac ccgggagatt
     4081 tggcgaaaat cgaacaagcc cattctttcg atcgatggag ccgggtgagc cgatgccgac
     4141 acatatcgat cggtcgcctt ggcctaaatc gttgacgata tcggcgattt tggccgcgac
     4201 cctttcctta ccggaggaca cctccgtttt cacgtgtttt ttcgctaaaa acttcccgtc
     4261 ttgggacacg agcgcggctt tgatgttcgt acccccgaga tccactccga tcgcattcat
     4321 ctctcgttcg cctgccttct attcttttcg aaagaggttc agaaacactg ttccgtaccg
     4381 atacgtatga agcctttcga atccggttat atccggaatg cttttcctat attctatcat
     4441 cagcaacgcg ttttttatca tcaaattagg tgaatccgca aaggtttgaa aaaacttttg
     4501 cgcgatgtcg tcgtgaaagg gagggtcgat aaagacaacc tcagattcga gccgcagctt
     4561 tctactcgaa attaataggt ttcgacggta cacccgcgcg cggtcctgaa cccctagttc
     4621 acgtagcgag gtcgcgatcg ctttttcgga ctctcgcgac agatcgtaga attccacgaa
     4681 cggcgcccca cggctaagcg cctcgattcc gacgacgccg ctccccgcac ataggtcggc
     4741 gaaatggctg gtggcgatat cgcgatgtcc cttcaagatc gaaaagcacg ccagccgtac
     4801 cttttccgac gtataccgcg taacctcgtt ggcgagcgct tttatccttc gacctttaaa
     4861 ctcgccacct gttatcgtta atgcgatcat tagaaaaacc ccttctctgt tcttcagggg
     4921 agcgtgtccg tccgttcaaa aaaccgaagt cccgcatgca attgctcgga aagcgtcttc
     4981 gcgcataggt ccaaaacgct gtggaattgc gcgagaaagg gactcagact aaccatcagt
     5041 ttcggtaaat ttttgaacaa taggcgtatg ctcatcaaag tataggtgtt gtcgggaaag
     5101 accaaaccga tctctccagg ttcaaaaagg tgggggaacc attcgatcaa gagctgtttg
     5161 atgagctttg aaatcaaccg atactcgctt aaagcgtatt tcggggtggc ttctttcggg
     5221 aacttgagag agagactcgc cagatcttct tcaaaatcga aacggtaacc cttcccgtgc
     5281 catttttcgt aggcgcccaa caggtttccc atcgcttcct ctgagaaaaa cccagcgacg
     5341 agcatttttt cgtgaccttg aaccgactcc ctcattaaga gcgttttaga cgttttttcc
     5401 tggcctaaaa acgcgaagga gggcacattg accatttcgc ctcccaaaat ggagtagaag
     5461 catttgactt gccctttctc cggaagtgta tcggtgtaag cccgaaagag ctcccggtac
     5521 aaatcgaaga aaaagggcgg ggtcgacatc tccgcagagc cgtattttct gatgagctct
     5581 cgcacattcg atccaagacc gataacgatg ggcccctgtc tttggcttaa aatatacaga
     5641 ggcgggtagg cgggagcgct gaccacgaac cccaagtagt cttcggaacc gcgcttcccg
     5701 atcttttggt agatagaatg gaaatcgata ttcgataacg cttttacgta aaaaacttca
     5761 aaggctttta ggatctctgg cggacctaac ttgattaaag accccattaa aacctcttaa
     5821 tcgacgagat cccaatggag cggggttcca aatcgtatgt cgactcgagc cttttttccc
     5881 aggatatccc tcaaaaaaac gggatgtagc ccgtatcccg gtcgaatcga ccgcacgttt
     5941 tccgaggtga atagctcccc ggcttccata tctttgatta caaagagaga ccggctgaaa
     6001 acccttcctt ttttcaacgt gctttcagcc acggtgtagt tgacctcccc gagcgccttc
     6061 tcacattctc ggatcccctg caccatctgc gtaaactctt ctggctccaa agaaaacgcg
     6121 gcgtccgcgc caccggcgct gcggtccagc gtaaaatgct tctcgataac ctttgcgccc
     6181 aacgcaacgg ctgtgagcgg caccgtgatg ccgagcgtat gatcggacaa gcccggaacg
     6241 actcggaagg tttctgccat attggggatc gttctcaggt tcgccgcctc cagcggggcg
     6301 ggatactcgc tggtgcattt caagagaacg atatccgcaa tccctttgtt ccgtagggtt
     6361 tttagtgcca gatcgatatc ggatatcgtc gcgaccccgg tggagaagag aacgggtttt
     6421 ttcttcgtgg cgatgtattc gatgagtggg atgtcggtaa tctcgaagga agcgatttta
     6481 taggctggca cgttcatccc ttccaagaag tctaccgctt tggtgtcgaa gggggtggag
     6541 aaacagatca tcccctcctc ttccgctatc tgcttgagtt ttggctgcca ttcccaagga
     6601 gtatacgctt tttgatacag tcggtaaagt gtctgcccat cccatatggt cccttgtttt
     6661 aaagaaaagt actcgttatc gcagtcgatc gtgaggctat ccggagtgta cgtttgcatt
     6721 ttgatggcat ccgccccagt ttttttcgct gctttgataa tcttcaccgc cacatcgaaa
     6781 tcctgctggt gattggccga catctccgcc acaatgaaaa ccggcgcgtg atccccgatc
     6841 agccgttctc cgatgcgaaa ttgcatccta ttccctcctt tttgcttcct accgcaccct
     6901 cccgaagccc ttcgctcgat ttacagtaaa tgtatctagg ggtttgcgga aatatcggat
     6961 ctacttcatc cgttatcggg tcttccgaaa acttgtttca ttataccaca gcccaccgcc
     7021 ctttatgcta caataggcgg tatgaaggca ttttggaaac tcttagtacg tcatttcccg
     7081 taagcttcta gggggggtta tgcacaagac gtttttgatc gtcgcttcgg tagaagccca
     7141 tatcttcggt ttccatctcc cggtattgac ctactttcag gagagggggt tcgaagtcca
     7201 tatcgctacg aggttcagta aacgactagg cttttttgag tcacaggggt atatctgcca
     7261 cgatatcccg ttttctcgtt cccctttcgc gttcgacaac ggggccgcgc tgcgcgcctt
     7321 aacgaaactc tttcgtgcta gacgatttga cgcgatacac acccatacgc cggccgcatc
     7381 ctttctctgc cggtgggcag cgcaacggac tcggcaaccc tatgtggctt acaccgcgca
     7441 cggccttcac gtctaccgtg gcgcacccat ttcccatcgt ctactctatg ggacagccga
     7501 acggcttgcg gcacgttgga ccgacacgct cattacgatg aaccgggaag ataccgactg
     7561 ggcaaaagag aacctccgcc tgaagaaggg cggctcgacg gtctatatcc ccggtgtcgg
     7621 gatcgacctc gaacggtatc aaatggccgg gttcgaccgt gcggaattta gaaaatcgct
     7681 cgatgtcttt gaagacgcgt tcatcctctc gttcatcgga gagctcaaca aaaacaagaa
     7741 tcagctgatg cttatagaag cctgtgagcg tttcaagccc tccgagccca tccgcctcct
     7801 cctcgtcgga gaaggcccaa tggaggaaac ctaccgccgt tacgtccaag agcgcggttt
     7861 aaacaatatc cattttctcg gttttcgaga tgatatcccg aagatactcg ccgtaagcga
     7921 tgtgatcgtc ttgacctcta aacgagaagg actcccccgg tgcgtgatgg aagggatggc
     7981 tgcggggaaa ccgatcctgg gcacaaatat tcgggggaca cgggatctcg tgatcgacgg
     8041 agagaacggc tttttggtcg aagtcggcga tgtgggcgcg ctaaccgaaa aaatccgaga
     8101 gctttacact aacgcccccc tacgccttca aatgggagag cggaatctaa agaggataaa
     8161 agacttcgct cttccgaagg ttatggatgc cttggacaaa gtgtacatcg atgccggttt
     8221 attcaaaaca gaagaagaac gtggtcgcta aaaaccctct acccggatgg tgccgttgga
     8281 ggtcgccaga tctagacgga agcccccggt gttgaccctt cccgacttca ggtttttctc
     8341 tgattgtgtc aacaaaaccg ataacccgaa aaaatcaata ctcccattgc tcgtggaggc
     8401 cctgaagtcg aaggctgacc ttgacggaac cctcaggctg attctgccgt tcgaggtgct
     8461 tatcttgcaa tcgccatttc ctgcgaacgc gagatccccc tggatagtcc cgttggaggt
     8521 actaaacacg ttatttcccc cagggagttc cacacgataa aggtctatcc cgccgttggt
     8581 ggtgtcggcc tgtatccggc cgcgtgtatt ccgaagatcg acccccccgt tggtggtcgt
     8641 cagttgtatg ctcgtttcga ggttttgcgc cttcaccgcg ccatttgaag ttcttccggt
     8701 gatgctggcc agggcgttag gcggaaggta gacgacaaat ttcgtgccat aagaggcttt
     8761 ggtgaaaaaa ttcccgtcag agggagattc agacttgatg atcaaacgat cgccctgcaa
     8821 atcatagctg ataatgatct tcttgatctc ttcatccaga ttgccgaggc cttgaacgcg
     8881 tttttcaccc cgtacgctga tcgaagaacc ctcggtcccc aagaattcga tcgctccgtt
     8941 tttggtctga acgacaagac tttttatcga ccctaaggcg atatctttcg aaaagctttc
     9001 tttcaccgtg atgaggttgc agcccgataa taaccacacg atagcaagca ggatccccat
     9061 aacaaaggct cttttcatag tatcacctcc acctgaccgc ttgtttcaag gctcctctgg
     9121 aatgatgatc cacgcgagca gatacatcag gattcctaaa ccgatgaagc acgtgaccgc
     9181 ccaaaccacg cgaaccggat tcgggtccac ttcgaagtaa ttgccaatgc caccgcaaac
     9241 tcccgccaat ctgcgatccg ttctcgaccg ataaagtctt ttataatccg ccatactcct
     9301 tcgcctcact cgactctcta tttatggggt tactcatctt cctcgaaggc gttgctctat
     9361 ccttcgtttg ccgtcgatag tctatcacat ctcagttaaa attcctttga agatattcct
     9421 tgattcgaat agccaatgct tgatcccgca caaccgcggt gatttctgaa acctcggctt
     9481 tagagatgtt tttcaagctg tgaaagcgct tcaataactt gatttttctc accttaccaa
     9541 cgcctggaat atcctcgagg gcggtttttt ccatccggtc atctcgtaat tgcgcgtgaa
     9601 aggtgttcgc gaaacggtgg gattcgtctc gcacgaagac caaaacgcgt aaagaagggc
     9661 tgttttcagg cagcttgagt gcgacgcgat cgtcgggaaa gacgatcgtc tcttcctctt
     9721 tcgccaagcc gatcatctga tagtcggtga tatggagctc tttctccaac acttccttta
     9781 ccgcgcccac ctgtccgatt ccgccgtcta tgagcaacag ctccggcaac gggtgtttag
     9841 agtacctccg tctggtaacg atacgcaacg cctcaaaatc gtcgggttgg gtcagttgac
     9901 tgattcggta cttccggtag cggcttttat cgggaaaccc gtttgaaaag gagacgacgg
     9961 aggccactgt gtacaaccct tgcgtatgcg cgatatctat cccctctatc gtagccgggt
    10021 atttttttag tcccaactgc acctgtagaa gcttcaggga ttccacagac aatcgctttt
    10081 tctccacctc tttgatcagg ttgttgctcg cgatttctaa gatcttctct tctccgacag
    10141 tcctcggagg accgatataa ctcacgtcga aaagcccctt gatccgctct tctgggatcg
    10201 ctccggggtt caagataatg ctgtccggta tctccgagtg gcggccataa tagaattgac
    10261 tcaagatctc ttcgacctcc aacaccccgt cgatgtctag agttaggcag ccgaagacgg
    10321 ctccatcctt catcctgaat acgactgtct taccttcagc aaggtctatg gcaaaaaagt
    10381 cttgattgat gttcttgggc aatgtgatgt attgaggaaa gaacagggta tccagttttg
    10441 tcagcagatc tctcacgatt tttgctttct catatagtag gtgccccgaa taaaacgcca
    10501 tcttttcttt cataagcgtt tttaacgagt gtatgttccc tttcaaaaac cgctctacct
    10561 tttttagctg ttcattgtag tcttcttccg aaataaaagc gcaaggcgcg ctgcaccgcc
    10621 ccagctgata ctcgaggcac ggtttgcgaa ccttactcaa atcgtagtca caagggcgga
    10681 tcccgtaggt tcgatagatg atctcaaaca cctgacgaac catccctacc gagctgaaag
    10741 ggccgtaata ttttcccttt tccactttgt cgcggaccac cttgatcctc gggaacttct
    10801 cctgtgtgat caaaacgtac ggatagaagc gattgtcttt cagcatcgtg ttgaaagcgg
    10861 gtttattctc atagatgagg ttggcctcca gcataaaggc ctctttttcc gaactcacga
    10921 ggatgtactt caactggtca gacctctgaa ggatgccgct caccttgtgt ctttcgccgt
    10981 tcgtttggcg aaaataagat aggatgcgtt tcttcaggct tttcgctttc ccgatgtaga
    11041 tcggcttgtc ttgctgtgaa aacacgtaga cgcccggctc gtcaggaaaa cgaagcgcga
    11101 cctctttcag cggatgttct aaaaactcat tgggctctag atccacgacc atcacgcctt
    11161 tatttggggt aagtgcgctt ccatatcgat tccaagtatt ctctgatctt tttctctttc
    11221 ccgatgaagg aaggcacgta aaacctttgc tcttgaaggg attcgggaag gtagggttcg
    11281 cgaatgaaac cggtgtcgct atcgtgcgga tatagatagt ctttaccgta cccggactct
    11341 ttcataaaag gggtgaccgg gttacgtagg cgaaagggaa ccggcaggtc ggggttcctc
    11401 gccacttctt ttttcgcgct ctcgtaggct acgtagatcc ggttgctctt cggcgcgatc
    11461 gccatataaa ccgccgcctg ggccagcgaa gtggtgcact cggggagtcc gatgtaatgc
    11521 gctgcttgaa aacagctgac ggcaacgctc atcgcttgag gttcggcaag tccgatatcc
    11581 tcgctcgcga accgaacgat ccggcgcgcc acatacaagg ggtcctcgcc cgattccagc
    11641 atacgcgtca aataataaac ggcggcgtcg gggtcgcttc cccgcatact tttaatgaag
    11701 gcagagatca gatcaaagtg ttcgtcgccg tgcttccgat atttggccgt cgatttttgc
    11761 aggaaccgtt cgatcaccgc gtgcgataag acggcgattc cggattgccg catgccaaag
    11821 aacaacactt ccgcgtaatt taagatcgta cgggcgtctc ccgaagacca atccaccaac
    11881 acattcagcg cgtcttcttc gacggtgatc tcgttattgg ggtccaaagc cgcttttccg
    11941 ttcaaaaaaa tcttcgcgat cgcctgtttc gagagttctt gaaaggtaaa gaccttgcat
    12001 cgggaaagca gtgcggggtt gatctcaaaa ctgggatttt ccgtcgtcgc tccgataaag
    12061 acgacctctc cgttttctat atagggaagg agcacgtctt gttgcaggcg attgaatcgg
    12121 tgaatttcgt ctacgaagag gatggtgctc tgtttggaca aggcgaagcg tttggaggct
    12181 tcatccaaaa tcggcttcaa ttgagacatc gtcgaccttg cggccgaaaa atgcgcgaat
    12241 ggatgacgag tccgttcttt aatgataccc ccaacggtcg ttttgccgca tccgggaggg
    12301 ccgtagaaaa tggcggagaa gagctggtca ttttcgatcg ccttccggag cacactccct
    12361 tgcgagaaga ggtggtcttg tccgagcacc tcgtccaggg tacgcggacg aatccgatgg
    12421 gctaacggga cgtcttcgcc ctgctcccaa agaggttttt gagacatacc ccagtcctac
    12481 gttgtcttct cgaaataacg gaccgtttca gaaatcccct ccgtcaaacc catcagagga
    12541 gaccacccca gcgtggtttt ggctagagag ctatcgagca cgcttttcct taggtcgccc
    12601 ggtcgggccg ggccgtgaaa cgcctcttgc cgataatggg tttggtcgcg taacagaccg
    12661 aacagtcgat tgacggagag acccgatccc gttccgatat tcaaagccag gccatccgcc
    12721 cgatctatcg ccagaaggtt ggcgcgtacg atgtcttgaa cgtaaacata atcgcgaatg
    12781 tattctccat ccccattgat cgtaaccggt tggccgcgca acatcttttg caaaaaaatc
    12841 gcgacgacgc cggcctctcc gtgggggttc tgccgaggac catagacatt cgcgtacctt
    12901 aaggaagtcc atctgagccc gtgttctttc tcgaaaaaag ccaaatactt ttctcccgtc
    12961 aatttggcaa taccgtaggg agagaagggc gcgcagggag aggtttccgg ggtgggtaaa
    13021 acgggcgggt tttcaccgta catcacgcca cctgaagacg tgtaaatgat tttcttcacc
    13081 gcttcccgca cgcatgcctc cataatacgg atagagccta cgacgttgat gtcggcgtca
    13141 aaggccggtt ctctcacaga acgggaaacg ctgatctgcg cagcaaggtg aaagacgtag
    13201 tccggtttat ggctcgaaaa ggcctctcta actagctccg cgtccgagac cgatcctttg
    13261 aacagccgga tgcccttcgg cgggaggttg tctagagaac cggttgaaag atcgtcgatt
    13321 acggccacat cgtattcttc ctcgatcaac gcgtctacca aatgagagcc gatgaacccg
    13381 gcgcctcctg taaccaaagc tcttttcatc acgaccgcct atcccggctt ttcttgaagg
    13441 aatctttcca gatcgttata agcctctggt tccccggttt ttatcttctt gaggatcgtg
    13501 atgaaccggg cgctccgctc ttcggtccgt tggtccaaga agtgttcgat gcggcaggac
    13561 aggttactgg atacattcgc cggcatcccc accaagtcga cgaaaaaagc gtccagaagt
    13621 tgatggactc ggatcagcgt gatcgcacgc tttttgccct cttccgaaag atagatgaga
    13681 ccgtatttct cgtaattgac gtagccgaga tctttcaatc gcttcatcgc gttcgtgacg
    13741 ctgggaagac tcacaccgac gctctcggct atgtctttta ctctcgcgac gggagccgct
    13801 tgcgtcagat cgaatatcac tttgatatag tcttcgagtg cgctggtgag cactttcttt
    13861 cgtccgcgag tcaattcaca ccaccgcctt tcaagttgtg ctcggtatcc aaatgttata
    13921 cttctgcact ggatttgaga tcgttttact gtccatccgg acaattttcg tggttccaaa
    13981 actgaaaaac actaaatagg tgttctcgta gtttccggag ctaaccttaa agttctgagt
    14041 cacttcaaag gtaatgtttt ggatagcttg ggcgggtttt ttaaagtaat agatcatctc
    14101 tcgggagagt ccgggagaaa gggtgttttg gaaaacaacc tttccgctag aagactccac
    14161 gctgacccaa accggtttgg acccgctgat aaaaaaacgt acgaacccca ctttatcgaa
    14221 agcgcgcgat tcgcttaatg cgatcatatc gttgagcccg ataatctgcg aatcgatgtt
    14281 gttcgaaacc accaggtatt tgtcgatgat cccgttcgga ccaagatagg cccgatcttc
    14341 cataatcatc ttgttcaaag tctgaaaacg ctcttctgat ttctgcttca atgcgtccaa
    14401 ctgggtcgcg tatgtgtttc ggaactcatg aagcttcata taactgaaga tcgaaaaggc
    14461 gagcgccaca aaaaaaagaa aggtccagac gaaagtggga ggctgttttg tgacaaggcg
    14521 cttcgttccg gttattgtcg cgcgcttggg gccatcgctt tctcgtgtcg ctggggttcg
    14581 cgtcttgggt tccgggaccg gcgtttcgga ggtttcccct tcctttagcg gcgttttccg
    14641 actcgatttt tcttttttac gcaaacgcgc acgctcttcg tagtaccgct tgagcggttt
    14701 ttcttctatg ctgacgggta cgctctcgac ggaggttgca ggtttttttt ctttcgtgcc
    14761 cggtccaggg atataggcca ttacacagcc ctccttagta tctcttcgct cttgtccaga
    14821 atgccctgtt cttcttcata gaaagcctta aagctttttc ggcacttctg tataatgttg
    14881 tccacttttt ttatgttgat cccgtacctt cgggctatct ccgaataggt atagctctcc
    14941 gtgtacatca cgaagatact catctcctcc tcagacagcg acgctttcat acgctcggtc
    15001 gtcttttcga ggagcaggtc cttcagattg atcgcttcgc tctcttgcgt agaaaccttc
    15061 gtcatcatcg gctcttcctc ttccgcttct tccgaagaga catagtcgaa agagtaggat
    15121 tccgtcagta tcttgttttt cttccgattc aagaatgtca gaaaggtctt aatctcggaa
    15181 tggatgttcg tccaggcgaa ggtgttaaaa tttgcgtctt tgtcggtcct atagttataa
    15241 atcgccttaa tgagccctac ataaccgctt tgaattatat cacttttttc cgcccactga
    15301 ccgtaatact gattagcaaa ctttaaaacc atgttttcgt atctctccac caagagggag
    15361 aacgcctctt ccaccccgtc ttgcgcgtat aggaccaat
//
LOCUS       DAOPXN010000043        15398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_3910_length_15398_cov_5.513980, whole genome shotgun sequence.
ACCESSION   DAOPXN010000043 DAOPXN010000000
VERSION     DAOPXN010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15398
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3910_length_15398_cov_5.513980"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="PKM49_03795"
     CDS             <1..1425
                     /locus_tag="PKM49_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81968.1"
                     /translation="PDTNFREMGGGYVEKLARAFFVLTLVFAAAFVVFFLLYFRANNE
                     QTALVIERANLKQQVTTLNTELNRRGVYQQELERKILALETEKPEPAATQVEEIDRLT
                     AQLNTLQDELEKKNAQISLLSTSQVSAPSEREKALQEDIVRLKQSYDDEYAKLQLSLR
                     KKDDEIARLTKTLEAQGSGDDQVKSLQTLLNTTQKDLAKVQTDLDSAKRQVAEGQTEI
                     AALKTQIETYQKGNQTLVQKDQEIASLKTQVADLRAQSTQQEAQIKNLNADVLTLTKE
                     KESLYQQLTKERVYQPIPPGESDAVRYKYLILGEDALLAEKYKESAENFQRAQLKDLA
                     LDVMATVYLRKRDLAYQKAISLYYSEGFDHYKGNRFEQAVAPFLKAVSLAQEVKTDYD
                     DDVLYYLGLSYYQLKRYTDAETQLKAVYAMKESTFKVHALYYLVRVTMDAGKKSEALA
                     YANLLKGFSQYATFAREAIKTLGE"
     gene            1427..2788
                     /gene="glmM"
                     /locus_tag="PKM49_03800"
     CDS             1427..2788
                     /gene="glmM"
                     /locus_tag="PKM49_03800"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041571045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNY81969.1"
                     /translation="MRILFGTDGIRGIAGAELSAELAFKVGNAIALIEKERQRHKVAG
                     NPQLFVGKDTRVSCDMLCNALSAGAAAGGLCVYDCGVLPTPALAYITRLHGSAGVMIS
                     ASHNTYEFNGLKILRDGFKLPDAEEARIEKVVLSDGVDVCTHAGIGRVLDYSQARGEY
                     VRWVLGKYPDGRIGFPGKSAVRVVMDPGNGAAYRIAPEVLRVLGVEVLLVNDEPDGFN
                     INLECGSQHTHTLRRKVLETKADFGIAYDGDADRCIFVDDRGKLTDGDKLMAVTALYY
                     RDKGILTGNQIVATVMSNLGLETFLKENHIGMERTRVGDRYVLEKMLANDRVLGGEQS
                     GHVIFLDRSTTGDGLITSLTVLEAVAHFGLPLSRLTERIPEYPQILRNVKVNDRERVM
                     ESAAIKEAIEECKGALGDDGRILLRPSGTEPLIRIMLEGKDMDVICRIAEEIAAKVER
                     ADA"
     gene            2781..5648
                     /gene="uvrA"
                     /locus_tag="PKM49_03805"
     CDS             2781..5648
                     /gene="uvrA"
                     /locus_tag="PKM49_03805"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNY81970.1"
                     /translation="MLDSIVIKGAREHNLKNVSLAMPRNAFIVITGLSGSGKSSLAFD
                     TLYAEGQRRYLESLSSYARQFLGDMKKPEVDMIDGLSPAVAIDQKTVSHNPRSTVGTV
                     TEIYDYLRLLYSHIGIPHCSKCGRELSRMSVDEIVDILIKELGEEAKILILAPIAREK
                     RGEYKKDFEAFRKKGYSKIRVDGIIYDLEENIQIDKNQRHTIHLVVDRMRFQKDKDHL
                     QRLADSLELALKEGNGFVEIEEMDGHRSFLYSEDYTCPICGISIPSITPKIFSFNTPW
                     GACKTCTGLGYTMEVDPDLMIDPELSLEKGALKMYKEGYMYDAIARVAEFYGARLDVP
                     WKDLPKKARDAVLFGTQEKIKHRHEFSDGFYEMSRPFEGAYTNLVRRYRETQSAEVRS
                     WIESLFMRMRECEECKGRKLRPEALAVTVHGKNIAELTDLPIDAMARFLNTLPLTELE
                     EKIAGEVLKEISRRLTFLLDVGLDYLSLSRRASTLSGGESQRIRLATQIGSKLQGVLY
                     VLDEPTIGLHQRDNTRLIQTLIELKEIGNTVLVVEHDEQVIRSADYLVDMGPGAGVQG
                     GQVVFCGTVDELIRKPPSGSLTGAYLNKSRQIETKTRLRDTSGDRIRLCGAGQNNLKG
                     IDVEIPIGVLVCVTGVSGSGKSSLINETLYPILKNRIFFTRSDEGIHRDISGVELIDK
                     VINLDQSPIGRTPRSNPATYTKAFDAIRDLYTLTTEAKARGYKKGRFSFNVKGGRCEA
                     CEGQGQVKIEMHFLPDVYVDCEVCKGKRFNRETLEVKFKGKNIADVLDMTVDEAADFF
                     ENLGAIKGTLNLLQEVGLGYVKLGQPATTLSGGEAQRMKIASELRKKATGKTLYILDE
                     PTTGLHFEDVRRLLQVLDRLVDLGNTVLVIEHNMDIIKNADWLIDLGPEGGEKGGRLV
                     YQGPTRAIVNCSGSYTGFYLKQVLEGGSLDRVSSRPNASSF"
     gene            5665..6429
                     /gene="mazG"
                     /locus_tag="PKM49_03810"
     CDS             5665..6429
                     /gene="mazG"
                     /locus_tag="PKM49_03810"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNY81971.1"
                     /translation="MRTLRSDKGCAWDREQTHDSLKPYLIEEAYEVIQAIEEGDAQHL
                     KEELGDVLLQILFHAQIAEEEDAFSLAESIEHLNEKMIRRHPHVFGDSKAGYTYKQWE
                     EIKAKEKGRAKSSRIGEFNPALPALSMARRAQENASTVGFDWPDASEAFCKVEEEGDE
                     VKKLLNRSAHRAGVETGEDRADIELEIGDLLFSVVNVARLLHLDPEAALKKSTRKFIS
                     RFQKMERLLEAEGLVLEDQTLEFLDRYWEKAKADGV"
     gene            6419..8401
                     /gene="uvrB"
                     /locus_tag="PKM49_03815"
     CDS             6419..8401
                     /gene="uvrB"
                     /locus_tag="PKM49_03815"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNY81972.1"
                     /translation="MAFKINQEMRPSGDQPEAIERLITSIQRGHRFQTLLGVTGSGKT
                     FTMAHTIAALNRSALIISPNKTLAAQLYREFKDFFPENRVEFFISYYDYYQPEAYIPT
                     KDIYVEKEAQINDIIQRMRLSTLKSVLTRKDVIIVASVSCIYATGDPDDFKNINLFLE
                     VGKTYSRRSILSKLTQLQYERSEDLFSGGKFRWKGEALEIFPTYDEGGIRFEFFDETL
                     ERVYSFDPVSRKVIEEFDRMVVFPTREYITTEEKIRRALESIQAELEERIRTFKDQNK
                     LLEAQRIEQRTLQDMEMLKELGYCTGIENYSRHFDGRAPGEPPWTLLDFFGDDYLLFI
                     DESHISVPQIGAMYRGDRSRKLNLVEYGFRLPSAIDNRPLQYEEFLEKVHQAVFVSAT
                     PGEYERGISDTIVEQIIRPTGLVDPQIEVRTTQNQVDDLIDQIHETTQKKERVLVLTL
                     TKSMAERLSDYLQELGIRSLYLHSELGTIERVEVLKKLRKGEIEVVVGINLLREGLDL
                     PEVSLVAILDADKEGFLRSETTLIQIVGRAARNVNGRVIMYADKLTPAISRTVEETNR
                     RRKLQLAYNEKHGITPQTIRKKIDDDLFSEFRVEGEEQEFYSTETREVLKLAERIDRS
                     DYVALLEEEMKKAAQELRFEDAMRLRDELYRLKKVY"
     gene            8706..9050
                     /locus_tag="PKM49_03820"
     CDS             8706..9050
                     /locus_tag="PKM49_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNY81973.1"
                     /translation="MLNPALLETILKFREDRDWEQFHTPKNLAISITLEAAELLEIFQ
                     WTKETETPETVSRKRLEIEDEIADVFVYLLLLCHDLGIDPELAIYKKMKKNAEKYPVE
                     KSRGKSDKYTEF"
     gene            9083..9367
                     /locus_tag="PKM49_03825"
     CDS             9083..9367
                     /locus_tag="PKM49_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81974.1"
                     /translation="MLIDEGMFIQVFSIEKDKQEIQKERIINIFSSPYMKEPLVFVAK
                     DNLNKARSADLRNPEVLKDIEAVLSDAGVEYIDLVTRHVIRDTFTKYING"
     gene            9400..10794
                     /locus_tag="PKM49_03830"
     CDS             9400..10794
                     /locus_tag="PKM49_03830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY81975.1"
                     /translation="MRETLLLPAERKDREALIALSSRIWEGEDYLPKVFDDWLSEEDP
                     FLLLWDKNRERLIGCAHLAFFGLTAWLEGVRVDPAFQGKGYGGALFRGALLEAFRKKA
                     DRIVSLIYSGNVESLHLAKKHGFDSIGEPYLLEAEPLHEPWGKTEPPLPIHPREIGRM
                     RSAFCEFLIASNQYWHDGWLAYPEADHLKDRFLFSYPDGSVIAGICSRDPDNFTVSAF
                     TKPGEWLAQALEEIRRWAGHLGCQLVTLTLPQQMEGFLPLLEKHGFLPVYDEDSPGEL
                     PARVCLMEYNPYHWDAAIVQAQLGLSAEKMSNYTATSYRCGYGFPAVVECFPLQNGKP
                     FPTSHYLTCPHLRYEISRLEESGEIKRLAPLFDSEAMRLSHGAVRRLRWTRLRNLGME
                     KDLPESYTEALSKGIGGIRDTHHGKCLHLHTAVYLAGISNPAGQAVIDRLKADGLTLD
                     CKTMRCAEFWSAQF"
     gene            complement(10838..13276)
                     /locus_tag="PKM49_03835"
     CDS             complement(10838..13276)
                     /locus_tag="PKM49_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HNY81976.1"
                     /translation="MKIVIIGGVAGGATAAARLRRVNEKAEIIMVERNGYISFANCGL
                     PYHIGGVIKERERLLVSTPQLFRDRFRIDVRTRTEALRIDRENKRVVLKDLSTNREYT
                     ESYDRLLLSPGGVPIRPTLPGVADEGFFTLRNMEDMDRIKDWMAARSITRALVIGGGF
                     IGLEMVENLVEKGVSVSLVELADQVMVNMDKEMVAPIHQSLADHGVSLYLKTQTTGFE
                     TVGKTIKATLDNGTVIETEMVLFSIGVRPEVKLAKDAGLELGPRGIRVNRYMQTSDAD
                     IYAVGDAVETHHPLLNEPTVVPLAGPANRQARIAADNMLHPNRSSYEGPIGTSIAKVF
                     ELTAGATGLNEKQLKAKKIPYLKSYTINNSHAGYYPGAFPITIKLLYDPSGKVLGAQA
                     IGIDGVDKRIDLLAVAVKMGMNVRDLTTFELSYAPPYGSAKDPVNIAGYVASNVLDGA
                     LEVFFPEDIQTADPSVVFLDVRDPEEQALSALPVKNVQSIPLAQLRARLSELDRTKTY
                     VTVCAIGQRAYIAYRILKDNGFKNVKDLTGGMKLVEAFSEKEYGKAAKVSKALFPAEV
                     LVESTQKIKLDACGLQCPGPIMETYKRMLDMKTGDFLEVLATDPGFRKDIAKWAEKTG
                     NSLIKIDQNNGVTTAVLRKGDRVPEEPTASASRTGKTIVVFSNDLDRALASFVIANGA
                     LAMGKSVTMFFTFWGLNILRKPDKSSIQKPFIERMFGAMMPRGAGKLKMSKMNMAGLG
                     TRMIRSRMVEKKIASLEQMIQNAIKEGVHLVACQMSMDLMGIRAEELIDGVELGGVAS
                     YLAEAEDANVNLFI"
     gene            complement(13281..13748)
                     /locus_tag="PKM49_03840"
     CDS             complement(13281..13748)
                     /locus_tag="PKM49_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNY81977.1"
                     /translation="MPRPPFIRHSLLKYLSMVSEMTGYDFLRYCSKNGLTVSPGSVYP
                     LLKKLESEGFVASSEVGRKKTYRLLPAGQEWVENHLAGDTDRDTERALGIFQMTVSCQ
                     CGEIPKSLCDSLKSFLLRLGKTNWHSKEQLGKLLGLSESLAREIRTYKNNLEG"
     gene            complement(14427..15278)
                     /locus_tag="PKM49_03845"
     CDS             complement(14427..15278)
                     /locus_tag="PKM49_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81978.1"
                     /translation="MNGVKPASMNRTFQAMLADTSMTIYNPKNIAIWPEFRWPVIVFR
                     MRQFGAVVVNGLTPTQSLYTEASNIHAMMVDAMKKLFNSPTVAVDYVKRVENPNNKPT
                     DVADAIRDLVENKKVNYIVLYYIAHGNIEYMSVGGTGFNASQLETLMASYPGVKFILF
                     VETCHGGSWRDYFRDLSPRLLNLKMVIASTSRDQGAYPDWDVAHGQTDVNGNDDQWIE
                     FTSDFLLKMEYYTSEAHWGEVTGLTFPAFDNNILRLFWLCYQRAQNDSWILPQNTGHQ
                     SPQIFYP"
BASE COUNT         3779 a         4146 c         4203 g         3270 t
ORIGIN      
        1 ccggatacca attttcgaga aatgggaggg ggttacgtgg aaaaattagc cagagcgttc
       61 ttcgtcttaa cgctagtttt cgccgccgct ttcgtcgtct ttttcttgct atattttcgc
      121 gccaataatg agcaaacggc tttggtcata gaacgagcca atttgaaaca gcaggtcacc
      181 acgttaaata cggagctgaa ccgacgcggg gtctatcaac aggaactcga gagaaaaatc
      241 ctggcgctgg aaacggaaaa gcccgaaccc gccgcgacac aagtggaaga gatcgacagg
      301 ttgaccgccc agctgaacac gctccaagac gagctagaaa agaagaacgc gcaaatctcg
      361 ttactgtcaa cctctcaagt ctcggcacct tccgagcggg aaaaggcctt gcaggaagat
      421 atcgtccggc tgaaacagag ctacgacgac gaatacgcca agctgcagct ttctcttcgc
      481 aagaaggacg acgagatcgc gcggttgacg aaaaccctag aggcgcaagg ctccggagac
      541 gatcaggtca aatctttaca aaccttactg aacacgaccc aaaaagattt ggccaaggtc
      601 cagacggacc tcgactccgc caagcggcag gtggcagaag gccaaaccga gatcgctgcc
      661 ctgaaaacac agatcgagac ctaccagaaa ggcaatcaaa cgctcgttca gaaagaccaa
      721 gagatcgcga gcctgaaaac gcaggtggcc gacctgcggg cacagtcgac ccagcaagag
      781 gctcagatca aaaacctgaa tgcggacgtc ctcaccctga caaaagagaa agagtctctc
      841 taccagcagc tcacgaagga acgggtctac cagccgatcc cccccgggga gagcgacgcc
      901 gtacgctata aatacctgat tttgggggag gatgcccttc tcgccgagaa atacaaggag
      961 agcgcggaaa acttccagag ggcccaattg aaggacttgg ccctagacgt tatggctacg
     1021 gtctacttac gaaaacggga cttagcctac caaaaggcca tctcccttta ctattccgaa
     1081 gggttcgatc actacaaagg aaaccgcttc gaacaagccg tcgctccgtt cctaaaagct
     1141 gtctctttgg ctcaagaggt caaaacggat tacgacgacg acgttctcta ttacctgggg
     1201 ctttcctact accaactcaa gcgttacacg gatgcggaaa cacaattgaa agcggtttac
     1261 gctatgaaag aatcgacctt caaggtccac gcgctctact acctggtccg ggtgacgatg
     1321 gacgcgggta aaaaatccga agccctggcg tacgccaatc tattgaaggg cttctctcag
     1381 tacgccacgt tcgctcgaga agcgattaaa acgttgggag agtgacgtgc gtattctatt
     1441 tgggaccgac ggtatccgag ggatagcggg cgccgagctt tcggcggagt tggcctttaa
     1501 ggtcggtaac gcgatcgccc tcatcgagaa agaaagacag cgccacaagg tcgccggaaa
     1561 cccgcaactg tttgtgggga aggatacgag ggtctcctgc gatatgctct gcaacgccct
     1621 ctcggccggc gcagccgccg gtggactttg cgtctacgat tgtggcgtgc tccccacgcc
     1681 cgctttggcc tatatcacgc gactacatgg ctctgccggc gtgatgatct ctgcctctca
     1741 caacacctac gagttcaacg gactgaagat cttgcgagac gggttcaagc ttcccgatgc
     1801 agaagaagcg aggatagaga aggtggtact ttccgacggc gtcgacgtat gcacgcacgc
     1861 cggaatcggc cgcgtactgg attactctca agcgcgtgga gaatacgtgc gctgggtgct
     1921 cggcaagtac ccggacggaa gaatcggctt tccaggcaag agcgctgtgc gcgtcgtgat
     1981 ggatccgggc aatggcgccg cttatcggat cgcgcccgaa gtgttgcgag ttttgggcgt
     2041 cgaggtcctc ctggtgaacg atgagcccga cggcttcaac atcaatttgg agtgcggctc
     2101 ccagcacacc cacacccttc gccgtaaggt gctggaaacg aaagccgact tcgggatcgc
     2161 ctatgacggt gacgcggacc gttgtatctt cgtcgacgac cgaggaaagc taaccgatgg
     2221 ggataagtta atggcggtca ccgcgctcta ctaccgggac aaaggcatcc taaccgggaa
     2281 ccaaatcgtg gcgaccgtga tgagcaactt gggtttggaa acgtttttga aagagaacca
     2341 catcggaatg gaaagaacgc gagtcgggga tcggtacgtc ttggaaaaga tgctcgccaa
     2401 cgatcgggtc ctgggcggcg aacaatccgg acacgtgatc tttttggatc gaagcaccac
     2461 cggagacggg ttgatcacgt cgttaaccgt tctcgaagcg gtcgcccact tcggtcttcc
     2521 cctatcgcga ctgaccgaac ggattcccga atacccgcaa atcctacgaa atgtcaaggt
     2581 gaacgaccgc gaacgggtga tggaatcggc agccattaaa gaagcaatcg aagagtgtaa
     2641 gggcgccctc ggggacgacg gcagaatctt gttgcgcccg tcgggcaccg agccgctcat
     2701 acgtattatg ctggaaggga aggatatgga cgtgatatgt agaatcgcag aagagatcgc
     2761 agcaaaggtg gaacgcgccg atgcttgatt ctatcgtcat caaaggcgct cgtgagcata
     2821 acctgaaaaa cgtctcccta gccatgcccc gcaacgcgtt catcgtcatc accggtctct
     2881 cgggatcggg aaaatcctct ttggctttcg acaccctcta cgccgagggg cagaggcgct
     2941 atctggaatc cctatcctct tatgccagac agtttttggg agacatgaaa aaacccgaag
     3001 tggatatgat cgacggcctg tcgccagctg tggcgataga ccagaagacg gtgagtcaca
     3061 atccccggtc caccgtgggc accgtcaccg agatctacga ttacctccga ttgctttatt
     3121 cgcatatcgg cattccgcat tgttccaaat gtgggaggga gctgagccgg atgagcgtag
     3181 acgagatcgt ggacatcctg attaaagagc tgggagaaga ggcgaagatc ctgattctcg
     3241 ccccgatcgc gcgtgaaaag cgcggggagt acaaaaaaga tttcgaggcc tttcgaaaga
     3301 aagggtactc caagattcgc gtggatggta tcatctacga cctcgaagag aatatccaga
     3361 tagacaagaa tcaacgccat accatacact tggtcgtgga tcggatgagg tttcagaagg
     3421 acaaagatca tctgcagcgc cttgccgact ccctcgagtt ggcgttaaaa gaaggaaacg
     3481 ggtttgtcga gatcgaggag atggatggcc accggagctt cctgtacagc gaggactaca
     3541 cgtgccctat ttgcgggatc agcatcccgt cgatcacccc gaagatattt tctttcaata
     3601 ccccttgggg agcgtgtaag acgtgcaccg gcttgggcta tacgatggaa gtggaccccg
     3661 atctaatgat cgatccggaa ctgtcgctcg aaaaaggcgc cctcaagatg tacaaagaag
     3721 gatatatgta cgacgcgatc gcccgagtcg cggagtttta cggagcgcgc ctggacgttc
     3781 cttggaagga cctacccaag aaggcgcgag atgcggttct tttcggaaca caggagaaaa
     3841 tcaaacaccg gcacgaattc tcagatggct tctacgagat gtcgaggccg ttcgagggag
     3901 cctacacgaa tctggtccgc cggtatcgag aaacacagtc cgcggaggtt cgcagctgga
     3961 tcgaatcgct ctttatgcgt atgcgcgagt gtgaggaatg caaagggaga aaactgcgtc
     4021 ccgaagcctt ggcggtcacg gttcacggca agaatatcgc cgaactcacg gacctgccca
     4081 tcgacgcgat ggcccgtttc ttgaacacct tacctttgac ggagctggag gaaaaaatcg
     4141 cgggtgaggt gttgaaagag atttcccgga gactcacctt cttgctggac gtcggtctcg
     4201 actatctcag cctttccagg cgcgcttcca cgctgtcggg cggggagtcg caacgtattc
     4261 ggctggctac ccaaatcggt tcgaagctcc aaggggttct ttacgtcttg gacgaaccga
     4321 cgatcgggtt acaccaacgg gacaataccc ggctgattca aacgctgatc gagctgaagg
     4381 agatcgggaa caccgtgctg gtcgtcgaac acgatgaaca ggtcatacgc agcgccgatt
     4441 acttggtcga tatgggcccc ggggcgggcg tgcaaggcgg gcaagtggtc ttctgtggca
     4501 cggtggacga gctgatacgc aaacctccct ccggttcgtt gacaggggcc tatctaaaca
     4561 aatcgagaca gatcgaaacg aaaacccgac ttcgcgacac ctccggagat cgaatacgtc
     4621 tatgcggcgc aggccaaaac aatctcaagg gtatcgacgt ggagatacca atcggcgtgc
     4681 tggtttgtgt caccggtgta tccggatcgg ggaaatcatc gctgatcaac gaaacactct
     4741 atccgatcct caagaaccgg atctttttca cccgcagcga cgagggcata catcgcgaca
     4801 tctcgggggt cgagcttatc gacaaggtca tcaatctcga ccaatcgccc ataggaagga
     4861 ccccgcggag caacccggcg acgtacacga aggccttcga cgccatccgt gacctctaca
     4921 ccttaaccac ggaagccaag gcgcgcggat acaaaaaggg acggttcagc ttcaacgtca
     4981 aaggcggacg atgcgaagcc tgcgaggggc aagggcaggt gaagatcgaa atgcacttcc
     5041 tgccggacgt ctacgtcgac tgtgaggtct gtaaaggaaa gcggttcaat agagaaacgt
     5101 tggaagtgaa gttcaagggc aaaaacatcg cggacgtgct cgatatgacg gtcgatgagg
     5161 ccgcggactt ctttgaaaac ctcggggcga tcaaagggac gcttaacctc ctccaagagg
     5221 tcggactggg ttacgtgaaa ctcggacagc ccgcgacgac cttgtccggc ggtgaggcgc
     5281 aacgaatgaa aatcgcctcc gagctgcgaa agaaagccac cggaaagacg ctctacattt
     5341 tggacgaacc gaccacggga ttgcatttcg aagacgttcg tcggttgttg caagttttgg
     5401 accgcctggt cgatcttggg aacaccgtcc tggtcataga gcacaatatg gacatcatca
     5461 agaacgcgga ctggctgatc gacctcgggc ccgagggtgg agagaaaggt ggccgtctgg
     5521 tgtatcaagg acctacgcgc gcgatagtta actgttcggg gtcttacacc ggcttttacc
     5581 ttaaacaggt gttggaagga ggctctttgg atcgtgtctc gagtcgaccg aacgcttcaa
     5641 gcttttagca aagccgtcga aattatgcgc acgctacgtt ctgacaaggg gtgcgcttgg
     5701 gatagggagc aaacccacga ttcgctcaaa ccttacctga tcgaggaggc ttacgaagtc
     5761 atccaggcta tcgaagaagg ggacgcgcag catctgaagg aagagctcgg ggatgtgtta
     5821 cttcagattc tgttccacgc ccagatcgcc gaggaagaag acgccttttc cttggccgag
     5881 tccatcgaac acttgaatga aaagatgatc cgccgtcacc cccacgtttt cggagattcg
     5941 aaagccggct acacatacaa gcaatgggaa gagatcaagg caaaagagaa agggagggcc
     6001 aagagctcgc gcatcgggga gttcaacccg gcgcttccgg ccctatcgat ggcccggcgg
     6061 gcccaagaga acgcctcgac ggtcggcttc gattggccgg acgcgtctga ggccttttgc
     6121 aaggtggaag aagagggcga cgaagtgaaa aagctgctga atcggagcgc ccatcgggcg
     6181 ggagtggaaa cgggagaaga ccgcgcggat atcgaactcg agatcggcga cctgctgttt
     6241 tcggtcgtga acgtcgcccg actgcttcac ctcgatccgg aagcggcgtt aaagaagagc
     6301 acgcgtaagt tcatctcacg ctttcagaag atggaacgct tgctggaggc ggaggggttg
     6361 gtcttggaag accagacctt agaattcctc gatcgctact gggaaaaggc gaaggcagat
     6421 ggcgtttaaa atcaatcagg agatgcgacc gtctggagac cagccggaag cgatcgaacg
     6481 tctcatcacc tcgatccaaa gggggcatcg gtttcagaca ttgctgggcg tgacggggtc
     6541 cggaaagacc ttcaccatgg cgcacacgat cgcggcgtta aatcggtctg cactgatcat
     6601 ctcacccaac aagactctcg ccgctcagct ctacagggag tttaaagact tctttcccga
     6661 aaaccgcgtg gaattcttca tcagctacta cgattactac caacccgaag cctacatccc
     6721 cacgaaggac atctacgtgg agaaggaagc gcagatcaac gacatcattc aacgtatgcg
     6781 cctttcgacg ctcaagtctg ttttaacacg caaagacgtg atcatcgtcg ccagcgtctc
     6841 ctgcatctac gccacggggg accccgacga cttcaaaaac atcaacctct ttttggaggt
     6901 ggggaagacc tattcgcgtc ggagtatctt atcaaagttg acgcagttac agtacgagcg
     6961 gtcggaagac ttgttcagcg ggggcaagtt tcggtggaaa ggcgaggccc tggagatctt
     7021 tcccacctat gatgaagggg gcatccgctt cgaattcttc gacgagaccc tggagcgtgt
     7081 ctattccttc gacccggtct cccgcaaggt catcgaagag ttcgaccgga tggtggtgtt
     7141 tcccactcga gagtacatca ccaccgaaga gaaaatccgg cgcgcgttgg aatcgatcca
     7201 agcggagctc gaagagcgca tccgcacgtt caaagaccaa aacaagctcc tggaagcgca
     7261 gcgcatcgag cagcgaacgc ttcaggatat ggagatgctg aaggaattgg gctactgcac
     7321 cgggatcgag aactattccc gtcatttcga cgggcgcgcc cccggagagc caccgtggac
     7381 ccttctggat ttcttcggcg acgactacct tctcttcatc gacgaatccc acatctccgt
     7441 accgcaaata ggggcgatgt accggggaga ccgctcacgg aaattgaacc tcgtcgaata
     7501 cgggttccga ctgccttccg cgatcgacaa ccgcccgctc cagtacgagg agttcctcga
     7561 aaaagtccat caagccgtct tcgtgtccgc cacgccggga gagtacgaac gggggatttc
     7621 cgacacgatc gtagagcaaa tcatccgccc gacgggcctg gtggatccgc aaatcgaggt
     7681 cagaacgacg cagaatcaag tggacgacct gatcgaccag atccacgaga ccacccagaa
     7741 aaaggaacgg gtcttggttt taaccctcac aaaatcgatg gccgaacgat tgagcgacta
     7801 cctgcaagag ttgggcatcc gctcgttgta cctccattcc gaactcggca cgatcgagcg
     7861 ggtcgaggtg ttgaaaaaac tgcggaaggg cgagatcgag gtggtcgtcg gcatcaacct
     7921 gttgcgtgaa gggctggacc tcccggaggt ctcgctcgtc gctattttgg acgcggacaa
     7981 agagggattc ttgcgctcgg agaccacgtt gatccaaatc gtcgggcgcg cggcgcgtaa
     8041 cgtgaacggt cgcgtgatta tgtacgcgga caaactcacc ccggctatct ccagaacggt
     8101 ggaagaaacg aaccggagac gcaagctgca gttggcgtac aatgagaagc acggaatcac
     8161 cccgcagacg atcagaaaga agatcgacga cgatttattc tccgaattcc gagttgaagg
     8221 agaagaacag gaattctact ccaccgaaac gcgcgaagtc ctgaaattgg ctgagcggat
     8281 cgatcgtagc gattatgtgg ccttgttgga agaagagatg aaaaaggcgg cccaagaatt
     8341 gagattcgaa gacgcgatgc gcctccgcga cgaactgtac cggttgaaaa aggtgtatta
     8401 acgatggagc tttctcgaag gaccttggcc gcgcctggaa acgccctggg aacgcttggg
     8461 aacgccctgg gaacgccgaa cttcagttcg gcatgggtta ctgggaacgc caatctttag
     8521 attggcatgg tttctttact tttctgggaa cgcttgggaa cgccaatctt tagattggca
     8581 tggttttatt tttcctggaa cgtttgggaa cgccgaactt cagttcggca tgggttactg
     8641 ggaacgccac acttcagtgt ggcatggttt ctttccagga acgtcgtata aaggagaccc
     8701 gtatgatgct caaccccgcg cttcttgaaa ccatactgaa attccgcgaa gatcgcgatt
     8761 gggaacagtt ccacacccca aagaacttgg cgatctccat caccctagaa gcggcggaac
     8821 tgctggagat tttccaatgg acgaaagaga cggagacacc cgagaccgta tctcgaaaac
     8881 ggctcgagat cgaagacgag atcgccgacg tcttcgtcta cctgctactg ctatgccacg
     8941 acctcggcat cgatccggag cttgcgattt acaaaaaaat gaaaaaaaac gcggaaaagt
     9001 acccggttga gaagtcccgg ggaaaatccg ataagtatac cgagttctaa cctcatttat
     9061 aggaagaaag gcagggtttg atatgctgat agacgaaggg atgttcatac aggttttctc
     9121 gatcgaaaaa gacaagcaag agatccaaaa agaacgcatt atcaatatct ttagctcccc
     9181 gtacatgaag gaaccgctgg tcttcgtcgc gaaagacaat ttgaacaagg cgcgaagcgc
     9241 ggacctgcgc aatccggagg ttctcaagga tatagaagcc gttttatccg atgccggcgt
     9301 ggagtacatc gacctcgtca cccgacatgt gatccgggac accttcacaa agtacatcaa
     9361 cgggtaaaaa aacgacatcc tcaatccacc cgcacgccga tgcgcgagac tcttctcctc
     9421 cccgcagaaa gaaaagaccg ggaagctctc atcgccctca gcagccgcat atgggagggt
     9481 gaggactacc ttcccaaagt gttcgacgac tggttgtccg aggaagaccc gttcttgctc
     9541 ctttgggata aaaaccgaga aaggctcatc gggtgcgccc acctcgcctt tttcgggtta
     9601 accgcttggt tggagggggt gcgtgtggat ccggcctttc agggaaaagg ctacggtggg
     9661 gctctgttcc gcggagcgtt gctcgaagcc ttccgaaaaa aggccgaccg aatcgtgtct
     9721 ctcatctatt ccggtaacgt cgaaagtctc catttagcca aaaaacacgg gttcgatagc
     9781 atcggcgaac cctatctgtt ggaagccgaa ccgcttcacg agccttgggg aaaaactgaa
     9841 cctccgctcc ccatccatcc gcgtgagatc ggaaggatgc ggtccgcttt ctgtgagttc
     9901 ctgatcgcct caaaccaata ctggcacgac gggtggctcg cctatccgga agcggaccac
     9961 ctcaaagatc gattcctctt ctcctatcca gacggcagcg tgattgccgg tatctgctct
    10021 cgagaccccg acaacttcac ggtttccgcc ttcacaaagc ccggggagtg gttggctcaa
    10081 gccttggaag agattcgccg ctgggccggt catttgggat gtcagttggt gacgctcacg
    10141 cttccgcaac agatggaggg gttccttcct ttactcgaaa aacacgggtt tcttcccgtc
    10201 tatgatgagg acagcccggg ggagcttccg gcacgcgtgt gtcttatgga gtacaaccct
    10261 taccactggg acgcggcaat agtgcaagcc caactcggtt tgtcagccga aaagatgagc
    10321 aactacaccg ccacatctta ccgctgtgga tacggattcc cagcggtcgt cgaatgcttt
    10381 ccccttcaga acgggaagcc cttccccacc tcccattact tgacctgtcc gcatctgcgg
    10441 tatgaaatct cacggttgga agagagcgga gagatcaagc ggttggcgcc cctcttcgac
    10501 tcggaagcaa tgcggctttc gcatggagcg gtgcgtagac tccgttggac ccgcttacgt
    10561 aatctcggga tggaaaaaga cctgccggaa agctatacag aggctctgtc aaaaggaatc
    10621 ggcggaatcc gagacacaca tcacgggaaa tgcctgcatc tgcataccgc cgtctatctg
    10681 gccggtattt caaaccccgc cggccaagcg gttatcgacc gtttgaaagc ggatggcctt
    10741 actctggact gtaaaacgat gcggtgcgcc gaattttgga gcgcccagtt ttgatggaat
    10801 ccggcaggat aaaccggacg ctccaagctt ctcactatta gataaacagg ttgacgttcg
    10861 cgtcttctgc ttcggccaaa taggaggcga cgccgccgag ctccacgccg tcgatgagtt
    10921 cctcggcgcg gatccccatc aaatccatcg acatctggca ggcgacgagg tggacaccct
    10981 cttttatcgc gttctgaatc atctgttcca gagaggcgat cttcttttcc accatccgag
    11041 accggatcat ccgagtcccc agtcccgcca tgttcatctt ggacatcttc agtttccctg
    11101 cgccacgggg catcatcgct ccgaacatcc gctctataaa tggtttttgt attgaggatt
    11161 tgtccggttt tctcaagatg ttcaatcccc aaaacgtgaa aaacatcgtg actgacttcc
    11221 ccatcgctaa agcgccgttc gcgatgacga aggaggccaa cgcgcgatcc agatcgttgc
    11281 tgaacacgac gatcgttttt ccggttctgg acgcggacgc cgtcggctcc tccggtacgc
    11341 ggtctccttt ccggagcacg gccgtcgtta ccccgttatt ctgatcgatc tttatcaatg
    11401 agttaccggt cttttcggcc catttggcga tgtcttttcg aaaaccgggg tcggtggcca
    11461 acacttcgag gaagtctccg gttttcatat cgagcatccg tttgtacgtt tccataatcg
    11521 gtccggggca ttgtagcccg catgcgtcca acttgatctt ttgcgtcgac tccaccaaca
    11581 cttcggcagg gaagagcgct ttcgatacct tggccgcttt accgtactct ttctcgctga
    11641 aggcctccac aagcttcatc ccgccggtca agtcttttac gtttttgaac ccgttatctt
    11701 tcaagatgcg ataggcgatg tatgcgcgct ggccgatggc gcagacggtc acataggttt
    11761 tcgtccgatc gagctccgaa agccgggcgc gaagttgagc cagtggaata ctctggacgt
    11821 ttttaacggg taaagcgctg agcgcctgct cttcgggatc ccgaacgtct aagaaaacaa
    11881 ccgatggatc ggctgtttgg atgtcttcgg gaaagaagac ttcgagcgcg ccgtcaagga
    11941 cgttggacgc gacgtacccg gcgatgttca cggggtcctt agcggagccg taaggcgggg
    12001 cgtaggagag ttcgaaggtc gtgaggtcac gcacattcat ccccattttg actgctacgg
    12061 caaggagatc gatacgcttg tccacaccgt cgatgccgat ggcttgcgcg cctaaaacct
    12121 ttccggatgg gtcgtaaagt agttttatgg taatcgggaa agcgccggga tagtaaccgg
    12181 cgtgcgagtt gttgatcgtg taagacttca gatagggaat cttcttcgcc ttgagctgtt
    12241 tctcgttcaa accggtcgcc ccggccgtca attcgaagac cttcgcgatg gaggtgccga
    12301 tcggtccctc gtatgaagac cgattgggat ggagcatatt gtcggctgct atacgcgctt
    12361 gccggttggc cggcccggcc aagggcacca ccgtcggctc gttcaaaagg ggatgatgcg
    12421 tttccaccgc gtcgcccacg gcgtaaatat ccgcgtcgct cgtttgcata taacggttga
    12481 cccgtatccc tctgggtccc aattccaacc ccgcatcttt ggccagcttc acttccgggc
    12541 ggacgccaat ggagaacagg accatttcgg tttcgatcac ggttccatta tcgagagtcg
    12601 ctttgatcgt tttcccaacc gtttcgaaac cggtcgtctg agtcttcaag tacaaggaca
    12661 ccccgtgatc ggcgagtgat tggtggattg gcgcgaccat ctctttgtcc atattcacca
    12721 tcacctggtc cgccagttcg accaaagaaa cggatacccc tttttcgacg aggttttcaa
    12781 ccatttccaa accgatgaaa ccgccgccta tgaccaacgc ccgggtgatc gaccgagcgg
    12841 ccatccaatc tttgatacgg tccatatcct ccatattccg aagggtaaag aacccttcat
    12901 cggcgacacc gggtagggtc ggccgtatcg ggacaccccc gggagaaagc agcaagcggt
    12961 cgtaggactc cgtgtactct cgattggtgc tcaaatcctt caacacgacc cgtttgtttt
    13021 cccgatcgat gcgcaaggct tcggtccgcg tgcggacatc gatgcgaaaa cggtctcgaa
    13081 acaactgggg tgtggaaacc agaaggcgct cacgctcctt gatcacgccg cctatgtggt
    13141 aaggcaaccc gcagtttgca aaactgatgt acccgttgcg ttccaccatg atgatctcgg
    13201 ctttttcgtt cactcttctc agacgggcgg ccgccgtggc gcctcccgcc acgccgccta
    13261 tgatcacaat cttcatctcg ctatccctcc agattatttt tataggttcg tatttcacgc
    13321 gctaacgatt cggaaagccc caacaattta cccaactgtt ctttactgtg ccagtttgtc
    13381 ttccccaggc gaagaaggaa tgatttgagc gaatcgcaaa gggatttcgg aatctcgcca
    13441 cactgacaag aaaccgtcat ctgaaaaatc ccgagcgccc tttcggtgtc tctgtccgta
    13501 tcacccgcga gatgattctc aacccactcc tgaccggcag gcagcagccg ataggtcttt
    13561 ttccgcccga cttcggaaga cgccacgaac ccttcacttt cgagcttctt caggagcgga
    13621 tacacagagc cgggagaaac ggtaagccca tttttcgaac aataccgtaa aaaatcgtaa
    13681 ccggtcatct ccgaaaccat cgatagatac ttcaacaaac tatgccgaat aaagggtggt
    13741 cgcggcatct cttcctccta atatcgtcac tgatatatcg ttgacgatat tatatcacag
    13801 actattctga aaaatcaaga gagaagcggg agaactatct ctgctgggaa cgactgggaa
    13861 cgccgcactt cagtgcggca tgttttcgtt tttccggcaa cttctctggg aacgctgaac
    13921 gcctgggagc gtcggacttt agttcgtctt gtctactggg aacactggga acgccacact
    13981 tcagtgtggc atgtctattg ggaacacctg ggaacgccac acttcagtgt gacatgggtt
    14041 gtgttttatc ttgaaaacct caaaacaagc caacttgaaa gttggcagcc cttaaaaata
    14101 aaacaggggt tcgcacagag aacacggaga acacagaggg gggctcatcg cggggtaacc
    14161 aaagggaccc cgcgatacat cctttgcctt tctcgaaaga gggtatcgcg cctgggaacg
    14221 ccgcacttta gtgcggcatg tctactggga acgccaactt ttaagttggc atgccttaaa
    14281 aataaaacaa ggtttcgcac agagaacaca gagaacacgg agaacacaga ggggagattc
    14341 acgctgggaa tgccgcgcgc tcccagttaa gcccaataaa aaaacgggag acgtactccc
    14401 gctttttttg gaaatatatg gagtttttat ggataaaaga tttgtgggga ctgatggccc
    14461 gtattttggg gcaagatcca tgaatcgttc tgtgcgcgtt ggtaacacaa ccagaacagt
    14521 cttaagatat tgttgtcgaa agcggggaag gtcaagcccg ttacctcacc ccaatgcgcc
    14581 tccgaggtat agtactccat ctttaggagg aagtcgctcg taaactctat ccactgatcg
    14641 tcattgccgt tgacgtcggt ttggccgtgc gccacatccc aatcaggata ggcgccttgg
    14701 tccctactgg tggacgcgat caccattttc aggttgagca gacgaggaga tagatctcga
    14761 aaataatcgc gccagctccc tccgtggcag gtttcgacga agaggataaa tttcacgcca
    14821 gggtagcttg ccatcagcgt ttctagctga ctggcgttaa agcccgtccc acccacgctc
    14881 atatattcga tattcccgtg cgcgatatag tagagaacaa tatagttgac cttcttattc
    14941 tccactaggt ccctaatcgc atccgcgacg tccgtgggtt tgttgttcgg attctcgacc
    15001 cttttgacat agtcgacggc gacagtgggg gagttaaata gttttttcat tgcgtctacc
    15061 atcatagcgt gtatattgga ggcttccgta tacaggcttt gggtaggggt caggccgttc
    15121 acgacaaccg ccccgaattg acgcattcga aaaacaatca caggccatct gaactctggc
    15181 caaatggcga tgtttttcgg attatagatc gtcatcgagg tatccgccag catcgcctgg
    15241 aaggtccgat tcatcgaagc gggttttacc ccgttcacca ctggccatcc ttgagtttcc
    15301 actgagtaaa gaatccgccc gcttttaccg ataacgcaga tcctaccagg atgactgtag
    15361 aacgatcccg gccactcgtc cacgtagaag agccaccc
//
LOCUS       DAOPXN010000044        15099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4010_length_15099_cov_5.713640, whole genome shotgun sequence.
ACCESSION   DAOPXN010000044 DAOPXN010000000
VERSION     DAOPXN010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15099
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_15099_cov_5.713640"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PKM49_03850"
     CDS             complement(<1..943)
                     /locus_tag="PKM49_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY81979.1"
                     /translation="MFKMPETITYALPELIGDTNLFVGRKKEFDYFLRVWYNRLIANI
                     AQSHAIVARRKKGKTAFLQRLFNILWSCPETGVIPFYFSVREMKTNLAEFSKSFFAAF
                     VNHYLAYIKRKPEWVAFPKAFEALRGKIENKRLKETYEVIEAAERINNWGQMWEAASR
                     APCGIAAVQNVKIVQIIDEFQNINAYVLDERGNTIDSMSGTYLDLAEKKEAPLIISGS
                     EVHGLLEIVRRLTARFEENVLENLPEEEAKETIWKYAEIFHTKIDDVGVDKLWNLTGG
                     DPLYIKALFSSKHNTKNDYVQEDNIIEVYTQEITSGLI"
     gene            1279..1995
                     /locus_tag="PKM49_03855"
     CDS             1279..1995
                     /locus_tag="PKM49_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY81980.1"
                     /translation="MPSFRVEKIGLTRFVVIDSNRGPILVDTVPLAYRERFWRRLDRQ
                     GLAPSRIAALILTHHHSDHTGMAASLLEINPMIRLIFHANEEQALSSGRNQPTVYTNQ
                     KVENFSKKANLQPTFPPLKRRPHDIVFEEAQSPILRDWGVEATLLHTPGHTSGSVCLF
                     FDDGTLIAGDTFSNIPLNPFKTNPFPFVYEDMPALLRSWKWLLSKEPVTITPSHGRSL
                     SVSKVEKALKNQETQSNLSQ"
     gene            2133..2873
                     /gene="fabG"
                     /locus_tag="PKM49_03860"
     CDS             2133..2873
                     /gene="fabG"
                     /locus_tag="PKM49_03860"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNY81981.1"
                     /translation="MRLAGKVALVTGGARGIGKAICLLFAKEGANVAACDVLDEDLAQ
                     LEKEAAGLAGVVKGFHMDVTNREEVKAVVEKTVAEFGKLDTLINNAGITQDAFLHKMT
                     EDKWDKVINVNLKGTFNVTQAAIPYLKEAGSGSITTTSSIVGQFGNVGQTNYAATKAG
                     VIGMTLSWAKELARFKIRANAVAPGFISTPMTQVMPEKIIEYMVEKTPLKMMGEPEDI
                     AYAFLYLASDEARYVTGQVLGVNGGLTI"
     gene            2902..4257
                     /locus_tag="PKM49_03865"
     CDS             2902..4257
                     /locus_tag="PKM49_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNY81982.1"
                     /translation="MPKRRIPLSTKGEKTYHDILETAQSLFLDRGYHKTSVLTISKAV
                     GISPAAFYQYFDSKETVFRKICDRFFDFIFDFDFSSTAGDRPIEELLEKLLDHFFDLL
                     WENRGVYKVFREAEFIDLEIGKAFSDRLHQKLNGFLNRKTSPEESRACFWFLWGPIYY
                     LSGFWILWKGEPVSSQVRSTLKSFLLHGISPDEFMVNKEVYLNLGDAQTIAQQVKTKG
                     EKTRALVLEAAEALFGRNGYTGTGIHHISEAAQCSVGTVYVYFRTKEDILRELVKQTS
                     RELRHQIKAYAARFTDRRDQEIAAYCGFLHFFRNHRWMYAIVREAEFVDSSISISYYD
                     SLRKPYEIALQQAIDRGEVSFYEPEHLAAILMGIGHSIGFSLFLIEDRAYYDNRNVLE
                     PLSQLILYGLNRFMPSHLMQKPARKKTQKQTIKSEGAIGAETSPAEPLISSTKNREVV
                     T"
     gene            4254..5456
                     /locus_tag="PKM49_03870"
     CDS             4254..5456
                     /locus_tag="PKM49_03870"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNY81983.1"
                     /translation="MKRVYIIGAKRTAIGAFGGSFKATSAVQLGVVAAKAAMEQAKVD
                     PKDVCETIVGNILSAGMGMGPGRQVSVHAGIPVERPGFSVNMLCGSGMKAVMMGAVDI
                     VAREAEVVVCGGIENMNMAPYLLLQGRNGYRLGHGEVYDHAVLDGLTDVFNRYHMGIT
                     AENLVEKYQISREAQDVFALSSQEKAIRAIESGRFKEEIAPVVLKDKKGDTVIDTDEH
                     PRKGTTLEGLAKLKPSFKKDGTVTAGNASGINDGASMTVLASEEAVKRHGYTPLVELV
                     SWAQSGVEPSIMGIGPVGAIREALKRANLTLKDIDLLELNEAFAAQSLAVLADLKQEH
                     GVSQEWLDSRVNVNGGAIALGHPIGASGNRIIVTLIYEMKKRGAKLGLASLCIGGGMG
                     TAVIVRNV"
     gene            5472..6497
                     /locus_tag="PKM49_03875"
     CDS             5472..6497
                     /locus_tag="PKM49_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HNY81984.1"
                     /translation="MSRLDKIGIIGIGSYLPATYMTAKDISLATGIPEDVIDLKFGVS
                     RKPIPGPGDTTSYMGLQAAKNALEMAKVSPLDLDLVIWNGAQHKDYPCWLASLKVAYD
                     LGAQNAWGFDMEAMCGSMMAGMETAKSLMRNNDDLRTVLLVSGYRNSDLISLKEPDTS
                     FMFDIGAGGAAMVLKKGADRNILLESAFKGDGSFSEGCVVPVGGTKKWPPVAEDLDHF
                     HFKIVEDPKEFKTRLGEKTMPNFYGVIRQSLKKSGYTEKDIDYLAILHFKKSAHLAVL
                     DELGLKESQTTYLQDYGHLGQNDQVLSLEIGLREGKIRDGSLIVMVGAGLGFVWASSV
                     VRWGPSR"
     gene            6508..7809
                     /locus_tag="PKM49_03880"
     CDS             6508..7809
                     /locus_tag="PKM49_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNY81985.1"
                     /translation="MEVFAEAYKSKKTDLDHVLKMIRSDQVIVTGMCCMEAQSFLSEL
                     HTIADRVRNVQVYTCLNMKEYEFYRQPGFESSFFNNSWFHSAGIRKSLKEGILNSTYV
                     PNNLHEAGANLLSLGDPDIYIGMVSPMDEHGFCSLSLSVTYEKDVIERAKTVIVEVNP
                     NAPRTHGDTQIHISEIDYLYEVDYPIPTLPDALPSEIEKGIANHIAELIPNEATLQIG
                     IGGIPNAVSRLLLDKREIGIHTEMFTESMIDLFEAGVITNRKKTLWPGKAICTFALGS
                     KKMYDFVDDNPGVWLLRGHYVNDPYVICQNKNMVSVNTALMVDLTGQVCSEGLGTTHY
                     SGAGGQLDTHRGASMSEGGKGIIALESATRKGESKIVSIFKPGSPVTVPRHDLDWVVT
                     EYGAVRLRGKPVNQRAKLLIGIAHPDHRERLKKEAHEYGIF"
     gene            7840..9024
                     /locus_tag="PKM49_03885"
     CDS             7840..9024
                     /locus_tag="PKM49_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY81986.1"
                     /translation="MKKLLVLLLVAALLVSGLAFAEPGITKDKVLIGTFQALSGPVAY
                     IGLSMRKGMDAYFNWVNANGGVYGRKIELLVADDQFNPAKTVVEVKRMVEQDKVFSIV
                     GGLGTPGCLAVMKYLNDSKVPFVYQGSGALGLAVPPNKYIFSVQPNYYSVEGPIVAKY
                     LARNLGYKRIGMVYRAADDGKEELAGFQSWLVSNRLEKLLVKAIPVNPNATSFDNEIL
                     ELLGAGVDVVFLSMYGQQPPNFMIQAKQYGLDAKIVMSYPNSDIQVIQLSQGAAEGAQ
                     AMAWVWLGDADSEQFQRYLAIYQETFPQEIPNAYAAAGFIAGEVFTEGLRRAGQNPTR
                     EGLVQALETMSGWQGLISPIISYKPYSVGDNTCRVGLQAMYAMEVVNGLWEKVSDWID
                     SK"
     gene            9088..9855
                     /locus_tag="PKM49_03890"
     CDS             9088..9855
                     /locus_tag="PKM49_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81987.1"
                     /translation="MGFLEVNRITVKFGGLVAVSEIDLTVEKGEIHALIGPNGAGKTT
                     LFNVITRVLPPTSGEISLNGKNLLKLQPHQTVQIGISRTFQNLEIFRMMTVKENLFVG
                     YHNRVNTRILQEAFQTKGLRAEEEKVQEKAIAVSDFLGLKNRLNSFAGQLPYGLQKMV
                     EIGRALMIDPDILLLDEPAAGLNPSETQELKQIIRSIRKKGITILLVEHDMSLVMDIS
                     DRITVMNFGKKIAEGDPREIRNDPVVIEAYLGESKYA"
     gene            9848..10552
                     /locus_tag="PKM49_03895"
     CDS             9848..10552
                     /locus_tag="PKM49_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY81988.1"
                     /translation="MLELKGVKLKYGYVEALKGIDMRVEEHQITALLGANGAGKSTTL
                     RAVSCLNRIEKGEIVFLDEQVHRLLPSEIVRRGIVHCPEGRKLFPFLTVQENLLAGAY
                     TQKDRNQVRNFQERVFTLFPILKDRRRQMAGTLSGGEQQMLAIGRALMAAPKILMLDE
                     PSLGLAPKVVEQIFDTLVTLREEGTTIFLVEQNANAALHIADYAYILEIGKVVLEGEG
                     KALLKNENVKKKYLGG"
     gene            10553..11443
                     /locus_tag="PKM49_03900"
     CDS             10553..11443
                     /locus_tag="PKM49_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNY81989.1"
                     /translation="MSAVLLQQIILGLREGSLFALVALGLVIVHKTTGVVNFAYGHMG
                     MFAAYITFTFYTLLGFNLYLAVAIGITFAVFLGILTEKFLLRPIRHLSHSAMLIITLG
                     MLMILEGMALLLWKQDFKQLPNLITGVPYRISLGNSFLIITRQDVLIFLVTTAIVFGT
                     FFYLKYTKLGLAVRMVSQNEEASHLMGVKVGRVYGFAWGLGTGLSALAAILAAPKEQV
                     QPNMMLNLQIQGLTAAVLGGFDSLPGAIVGGVLLGIIEQLVAKYISVELKTALALMVI
                     IILLLVKPEGLLGKSTKRRV"
     gene            11446..13209
                     /locus_tag="PKM49_03905"
     CDS             11446..13209
                     /locus_tag="PKM49_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY81990.1"
                     /translation="MNAKRDFLIFAAVLIALPLFFLNQTFLLSIFGQVLIFSIAALGL
                     NILIGFAGQISIGHASFIAVGAYLSAILTKNYHIPFAVTVFLSGGLAGLFGLVLGFPA
                     LRLKGFYLAIATMAFVVMIEQLLGSWSYVGGHIGMRDIPNFDLFGYVLKSDLGKYYVI
                     AFCTFFLFLFGFNFLKSRTGRALKAIRESEFAARSAGVNVAKYKLTAFVISAIYAGIA
                     GALYAHTINYIAPTDFGLGVSINLLAMIVVGGLASFSGNFIGAILMIAMPFLFSRSNL
                     PLSVIFGILLVIVVLFFPRGLGYGVQLLNLKYFGIPAAWLRRKIRVSSVVGGKEMYVN
                     LPEGKMHYEIGGNPTGKPLFMVHGNFGSWRWFQPVMSRLEKTPFKGVAIDLLGFGDSE
                     KPEREISIPQYGQELAGFIDVFKEVKANLIGHSLGGAVIWDYAVNHPDRVNRILLIDP
                     APADGLVTPEEYYPALALYRNNRDLLKTALAGTIPSGDPDKLLDKLVNDAMVMDPRAF
                     TENARALERYNYLADLHKIQCPVLIVYGEKDLLITDKLLAETLKRLPNARVKALPGIG
                     HSVNVENPDLAVQIINEFFGG"
     gene            13209..14018
                     /locus_tag="PKM49_03910"
     CDS             13209..14018
                     /locus_tag="PKM49_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY81991.1"
                     /translation="MGVFLALRDGGSLYYETFGAKGDWIVFLNGIMMSVPSWKDFTGR
                     VSKHFRLLLVDFRDQGQSSKRAGPYQCAVHVPDLVELFDHLGIESVHMMGVSYGGQVA
                     LEFCRAHQNRLKTLELVTVLPKVSNYLVAMGEGWEVAAALNDGARFFSLTIPSIYSDT
                     FYAQRLEWLKNRQNAFRKTLTPEWFEAFIRLSQSANRFDCEDVLPTIRVPTLVLCAEK
                     DILTPAKEMKEMAKLIPSATVLEIPDAGHAAFLEKPEAFLTAIIGYIKSRD"
     gene            complement(14119..14523)
                     /locus_tag="PKM49_03915"
     CDS             complement(14119..14523)
                     /locus_tag="PKM49_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015006685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HNY81992.1"
                     /translation="MTKLGIIEGIGEAYELKLKEVGIKSVEQLLEACTSKKGRAELAK
                     KADISEKLLLKWANFADLMRIKGVGGRYSELLELAGVDTVPELAARKPENLYKKMVEV
                     NEQKNLVQKLPTAKQVENWTKQAAALPRILQY"
     gene            complement(14813..>15099)
                     /locus_tag="PKM49_03920"
     CDS             complement(14813..>15099)
                     /locus_tag="PKM49_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81993.1"
                     /translation="GIEIVIHVDPVCTHTEIVDRVYAHLCEIAGKIDPSLSIEHLRVI
                     RGRSRSSIRFSLGYPAGLPPFKMEETIRRLREDLQKDNQNSEYVIDDTNL"
BASE COUNT         3621 a         3947 c         4048 g         3483 t
ORIGIN      
        1 aaataagtcc gctcgtgatc tcttgcgtgt atacttcgat gatgttatcc tcttgcacgt
       61 agtcgttttt cgtgttgtgc tttgacgaga agagcgcttt gatatacaaa gggtctccgc
      121 ccgtgaggtt ccaaagcttg tctactccga catcgtctat cttcgtgtga aagatctccg
      181 cgtatttcca aatcgtttct ttcgcctctt cttcagggag gttctccagg acgttctctt
      241 cgaaccgcgc cgtgagtctt ctcacgatct ccaacaaccc gtggacttca ctccccgaaa
      301 tgatgagcgg cgcctctttc ttctccgcaa ggtccagata agtcccgctc atcgaatcaa
      361 tcgtattccc acgttcatcg agcacataag cgttgatgtt ttggaattcg tctatgattt
      421 ggacgatttt tacgttttga accgcggcga ttccgcaagg cgcccggctt gccgcttccc
      481 acatctgccc ccagttgttt attctttccg ccgcttcgat gacttcgtag gtctctttca
      541 agcgcttatt ttcgattttt cctcttaatg cttcaaaagc cttcgggaaa gctacccact
      601 cgggcttgcg tttgatatag gctaaataat gattcacgaa cgctgcgaaa aaggatttcg
      661 agaactctgc gagatttgtt ttcatctctc ttacgctgaa gtagaacggg atcacacctg
      721 tttcggggca gctccataaa atattaaata atcgctgcaa gaacgccgtt ttccctttct
      781 ttcgcctggc gacgatcgcg tggctctgcg cgatattagc gatcaatcgg ttgtaccaaa
      841 ctctcagaaa gtaatcgaac tccttctttc tcccgacgaa gaggttggta tcacctatca
      901 gctccggcaa ggcgtaggtt atcgtttccg gcatcttgaa catcgctttc acccgctttg
      961 tttatgtttc ataagattat agcatagaac gaaagactgg gagcgccaac ttttaagttg
     1021 gcatgtcttg gaacagaaga ggttatcgcg gggtcccgtt ggttgccccg cgataacccc
     1081 actcgggcat cggggcgtaa gcgctcctac tttttgcgac ggcgcgatac gcttttgaga
     1141 aggacatagg gtggatcgcg gggtcgtaga aactacttac cctgcgataa accacaaaag
     1201 catcgctccg gctgcgtggc tgtgccgcgc gctctatgtg atacaattaa gccgacataa
     1261 aaataagggg tgaagacctt gccgagtttt cgtgtggaaa aaatcgggtt aacgcgcttc
     1321 gtcgtcatcg actcaaaccg tggaccgatc ttggtcgata ccgttccgct cgcttaccgg
     1381 gagcgctttt ggcgtcgtct ggatcgacag ggcctcgcgc cttcgcggat cgccgcgctt
     1441 atcctcaccc atcatcactc ggaccatacc ggaatggccg cctctctgct cgaaattaac
     1501 ccaatgatcc ggctgatctt tcacgccaac gaggaacaag cgctcagctc cgggcgaaat
     1561 cagcctaccg tttacacgaa tcagaaggtc gagaactttt ccaagaaggc gaatctgcag
     1621 cccacctttc cacccctgaa acgccgaccg cacgacatcg ttttcgaaga ggcccagtct
     1681 ccgatcctca gggattgggg agtcgaggcc accctgttgc acacccctgg gcacacctcc
     1741 ggctccgttt gcctgttctt cgatgacggc accttgatcg ccggggatac cttttcgaat
     1801 atcccgctga atcccttcaa aactaaccct ttccctttcg tctacgaaga tatgcccgcg
     1861 ctgctccgct cctggaagtg gctcctgtcg aaagagcccg tgacgatcac cccttctcac
     1921 ggccgttcgc tctccgtatc gaaagttgaa aaagctctaa aaaaccaaga aactcaaagc
     1981 aatctctccc aatagaacgc atttttcgca taatcgggcg caagtgagga atctcgcggt
     2041 ggatttttct agaagaatgg tttatcatca aatatgacag gtgattcatt cgatagatga
     2101 tagatgattc agatattatg aggaggtgca ccatgcgttt agcaggaaaa gtcgcgctcg
     2161 tgaccggtgg cgcgaggggg atagggaaag cgatctgttt gctttttgcc aaagaggggg
     2221 ccaacgtcgc cgcttgcgat gtcttggatg aagacctcgc gcaacttgaa aaagaagcgg
     2281 cagggcttgc cggagtggtc aaagggtttc atatggacgt cacgaaccgc gaggaagtca
     2341 aagcggtcgt cgagaaaacc gtcgcggagt tcggtaaact ggatacctta atcaacaacg
     2401 ccgggatcac ccaagacgcc tttttgcaca agatgacgga agacaaatgg gataaggtga
     2461 tcaacgtcaa tctgaaagga accttcaacg tgacgcaagc ggcgattccc tacttgaaag
     2521 aggcgggaag cggctcgatc acgacgacct cctcgatcgt cgggcaattc ggcaacgtgg
     2581 ggcaaacgaa ctacgcggcg acgaaggccg gagtgatcgg gatgaccctg tcctgggcca
     2641 aagagctcgc gcgtttcaag attcgagcca acgcagtcgc cccaggcttc attagcaccc
     2701 ctatgacaca ggttatgccc gaaaagatca tcgaatatat ggtagaaaaa actccgttaa
     2761 aaatgatggg agaaccggaa gatatcgcct acgccttcct gtacctcgcc agcgacgagg
     2821 cccgatacgt gaccgggcag gttttagggg tcaacggggg cctgacgatc taacccccct
     2881 atcctgttag gagaaaaagc gatgccaaaa cggagaatcc ctctttcgac taaaggggaa
     2941 aaaacctatc acgatatcct cgagacagct cagtccctat tcctcgacag aggttatcac
     3001 aagacctcgg tcctgacgat ctcgaaagcg gtggggatct ctccggcggc gttctatcag
     3061 tatttcgact ccaaggagac ggtctttcgg aagatttgcg accggttctt tgacttcatc
     3121 tttgatttcg atttctcatc cactgcaggc gacagaccga tagaagagct attggaaaag
     3181 cttctggacc atttcttcga tctgttgtgg gaaaaccggg gcgtttataa ggtcttccgt
     3241 gaggcggagt tcatcgatct cgaaatcggg aaggccttct ccgatcgttt gcaccaaaaa
     3301 ctgaacgggt tcctcaatcg aaagacctct cccgaagaga gccgcgcctg cttctggttt
     3361 ctatggggcc cgatctacta tttgagcggc ttttggatct tgtggaaggg agaacccgtt
     3421 tcctcacaag tccgatcgac cttgaaatcc ttcttgctcc acggcatttc cccagacgaa
     3481 ttcatggtga acaaagaagt ctaccttaat ttaggcgatg cgcagaccat cgctcaacag
     3541 gtgaagacga aaggagaaaa aacccgtgcg ctcgtattgg aggccgccga agcgctattc
     3601 ggccggaacg gatacaccgg cacggggatc catcacattt ctgaagccgc tcagtgcagc
     3661 gtcgggacgg tatacgtcta ttttcggacg aaagaggaca tccttcgaga actcgtgaaa
     3721 cagacgagca gagaactccg ccaccagatc aaagcctacg ccgcgcgctt taccgatcga
     3781 agggatcaag agatagcggc ctactgcggg tttctgcatt ttttcagaaa ccatcgttgg
     3841 atgtacgcga tcgtgagaga ggcggagttc gtggactctt cgatttccat ctcctattac
     3901 gattccctcc ggaaacctta tgaaatagcc ctacaacagg cgatcgaccg gggtgaggta
     3961 tcattttacg aacccgaaca cttggccgcg atcttgatgg gcatcggcca ctcgataggc
     4021 ttttctctgt ttctgatcga agatagggcg tattacgaca accggaacgt gctcgaaccc
     4081 ctatctcagc tgatcctgta cggactcaat cgatttatgc cgtctcacct tatgcaaaaa
     4141 ccagccagga aaaaaacgca aaaacaaaca ataaaatcgg aaggcgcgat cggagccgag
     4201 acttcacccg cggaaccttt gatctcgtcg accaaaaatc gggaggttgt cacttgaaga
     4261 gggtatatat catcggagcg aaacgaacgg ctatcggggc ctttggcggt tctttcaaag
     4321 cgacgtcggc ggtacaactg ggagtcgtcg cggcaaaagc ggcgatggaa caggcaaagg
     4381 tcgatccgaa agacgtgtgc gaaacgatcg tcggcaacat actttctgcc gggatgggta
     4441 tggggccagg gaggcaggtc agcgttcacg ccggtattcc agtcgagcgg cctgggtttt
     4501 cggtcaatat gctgtgcggg tcgggtatga aagcggtgat gatgggcgcc gtcgatatcg
     4561 tggcgagaga ggccgaagtg gtcgtgtgcg gcgggatcga aaatatgaat atggcccctt
     4621 atctgctcct acaaggccga aacgggtatc ggcttggcca cggagaggtc tacgatcacg
     4681 ccgtcctgga tggattgacc gacgtcttca atcgctacca catgggaatc accgccgaaa
     4741 acctggtcga aaagtaccag atttcgcgtg aagcgcagga cgtcttcgcg ctgagcagtc
     4801 aagaaaaggc cattcgcgcc atcgaatccg gtcgtttcaa agaggagatc gcgcccgtcg
     4861 ttctcaagga caaaaagggc gatacggtca tcgataccga cgagcatccc cgcaaaggga
     4921 cgactctgga ggggctggcg aaattgaagc cgtcctttaa gaaagacgga accgtcaccg
     4981 cgggaaacgc ttccggaatc aacgacggcg cgtctatgac cgttttggca tcggaagagg
     5041 cggttaagcg ccacggatac accccgcttg tcgaactggt ctcttgggcg cagagcggtg
     5101 tggaacccag catcatggga atcgggcccg tcggcgccat acgggaggcg ttgaaacgcg
     5161 ccaacctcac cttgaaagat atcgatctgc tcgaattgaa cgaggccttc gcggcccaaa
     5221 gcctcgcggt cctggcggat ctgaagcaag agcacggcgt ctcccaagaa tggctggaca
     5281 gtcgtgtcaa cgtcaacggt ggggcgatcg cgttaggaca tccaatcggc gcgagcggca
     5341 accgcattat cgtcaccttg atctatgaga tgaagaaaag gggcgccaag ctgggattag
     5401 ccagtctgtg catcggcggg ggcatgggta cggccgtcat cgttcgcaac gtctaaagcg
     5461 ggaggttgcg tatgagcaga ttggataaga tcggaatcat cggtatcggg agctacctac
     5521 cagccaccta tatgacggcg aaagacatct ctttagccac cgggattccg gaagacgtga
     5581 tcgacctgaa gttcggggtt tcccgaaaac ccattccggg tcctggggac acgaccagct
     5641 atatgggact ccaagcggcg aagaacgcct tggagatggc caaggtctcc ccgctcgatc
     5701 tcgacctggt catttggaat ggcgcgcagc acaaagacta cccgtgttgg ttggccagtt
     5761 tgaaagtggc ctacgatttg ggcgcgcaga acgcctgggg attcgatatg gaggcgatgt
     5821 gcggttcgat gatggcggga atggaaacgg ccaaaagctt gatgcgtaac aacgacgacc
     5881 tgcgaacggt tctgctggtt tcgggctatc gcaactcgga tttgatcagc ttgaaagagc
     5941 ccgacacctc cttcatgttc gacatcggcg cgggaggcgc ggcaatggtc ctgaagaaag
     6001 gggccgatcg gaacatctta ctggaatccg ctttcaaggg ggacggctcg ttttccgaag
     6061 gctgcgtggt tccggtgggc gggactaaaa aatggccccc tgtcgccgaa gacctcgatc
     6121 acttccattt caaaatcgtg gaggacccga aagaattcaa aacccgtctc ggagagaaga
     6181 cgatgccaaa cttctacggc gtgatccgcc agtcccttaa aaaatcaggt tataccgaaa
     6241 aggatatcga ctacctggcg atcctacact ttaaaaaatc cgcccatctg gcggtactag
     6301 acgaattagg acttaaagag agccaaacca cgtatcttca agactatggc cacctaggcc
     6361 aaaacgatca ggttttgtcc ctcgaaatcg gactgcggga aggaaagatc cgggatggga
     6421 gtctcatcgt aatggtgggc gccggactgg gtttcgtttg ggcctcgtcc gttgttcgat
     6481 ggggaccttc tcgataggag gagagctgtg gaagtctttg cggaagctta taaatcaaaa
     6541 aaaactgacc ttgaccacgt tctgaagatg atccgctcgg atcaggtgat cgtcaccggc
     6601 atgtgttgta tggaagcgca atcctttcta tcggagctgc acaccatcgc ggatcgtgtc
     6661 aggaacgtcc aagtctacac ctgtttgaat atgaaagaat acgaattcta tcgacaaccc
     6721 ggtttcgagt cgtctttctt caacaattct tggttccata gcgcgggaat tcggaaatct
     6781 ttgaaagaag gaatcctcaa ctcgacttac gttccgaaca acctgcacga ggccggcgcc
     6841 aatctcctct ctttagggga ccccgatatt tacatcggca tggtgtcccc gatggacgag
     6901 cacgggtttt gttccctatc cctcagcgtc acctatgaga aagatgtgat cgagagagcc
     6961 aaaaccgtca tcgtggaagt taacccaaat gcgccacgca cgcacggcga cacgcagatc
     7021 cacatatctg agatcgatta cctgtacgag gtggattatc ccataccgac actgccggac
     7081 gcgcttccat ccgaaattga aaagggaatc gccaaccaca ttgcagagct gatcccaaac
     7141 gaggcgacgc tccaaatagg tataggcggc attccgaacg cggtaagcag gctcttgctc
     7201 gataaacgag agatcgggat acacacagag atgttcaccg aatcgatgat cgaccttttc
     7261 gaggccggcg tgatcaccaa tcgaaaaaag acgctgtggc cggggaaagc gatctgcacc
     7321 ttcgcgctcg ggtccaaaaa gatgtacgac tttgtcgacg acaacccagg cgtgtggctc
     7381 ttgcgtggcc attacgtgaa cgacccgtat gtcatctgtc aaaacaaaaa tatggtttcg
     7441 gtcaacaccg ccctaatggt cgatcttacg ggacaagtct gctcagaagg actgggaacc
     7501 actcattaca gcggtgcggg cggccaattg gacacccatc gtggggcctc gatgagtgag
     7561 ggtggaaaag ggattatcgc gctggagtcc gcgactcgaa aaggagaaag caagatcgtt
     7621 tcgatcttca aacccggatc gccggtcacg gtcccgcgac acgacttgga ttgggtcgtc
     7681 accgaatacg gggccgttcg gttgagggga aagccggtca atcaacgggc gaagctgttg
     7741 atcgggatag cgcacccgga tcatagagaa agactcaaga aagaagctca cgaatacggt
     7801 attttctaaa cgttaacaaa aaactcagga ggtggaagta tgaaaaagtt gctcgttttg
     7861 ttgttagtgg ctgcactact cgtatccgga ttggccttcg ccgaaccggg aataactaag
     7921 gataaggttc tgatcggtac gttccaagcg ctttcgggcc cggtcgccta catcggttta
     7981 tcgatgcgga aagggatgga cgcctacttc aattgggtta acgcgaacgg cggcgtctac
     8041 ggacgaaaga tcgaactgtt ggtggcggat gaccaattca accctgccaa gaccgtcgtc
     8101 gaagtgaagc gtatggtcga gcaggacaag gtcttttcaa tcgtcggcgg gttgggcacc
     8161 cccggctgtc ttgccgttat gaagtatttg aacgacagca aagtcccctt cgtctaccaa
     8221 ggaagcggag cgctcggttt ggccgtgccc cccaacaagt acatcttctc cgttcagccc
     8281 aactactact cggtcgaggg accgatcgtc gcgaaatacc tggcgagaaa cctgggttac
     8341 aaacgcatcg gtatggtcta cagagcggcc gatgacggaa aagaagaact cgccggattc
     8401 cagtcctggc tcgtgtcgaa ccgcctggaa aagctgttgg tgaaagcgat acctgtgaac
     8461 ccgaacgcga cttctttcga caacgaaatc ctcgaattgt taggcgcggg cgtggatgtg
     8521 gtattcctgt ctatgtacgg gcaacagccg cccaacttca tgatccaagc gaagcaatac
     8581 ggcttggatg ccaagatcgt gatgagctat cccaactcgg acatccaggt gatccagttg
     8641 tctcagggag cggctgaagg cgcccaggcg atggcttggg tatggttggg cgatgccgat
     8701 tccgaacagt tccaaaggta cttggccatc tatcaggaaa ccttcccaca agagattccg
     8761 aacgcctacg ctgccgcggg ctttatcgcc ggtgaagtgt tcaccgaagg tctcagaaga
     8821 gcggggcaga atcccacacg cgaagggttg gtgcaagcgc ttgagacgat gagcgggtgg
     8881 cagggcttga tcagccccat catctcctac aaaccctaca gcgttggcga caacacatgc
     8941 cgggttgggt tgcaggcgat gtacgcaatg gaagtcgtta acggcctttg ggaaaaagtc
     9001 tctgactgga ttgattctaa ataagtatgg ggaaacgggg gctctgcgaa gagcccccgt
     9061 gttctccgtg aggagtgagg tgacgtcgtg ggttttttgg aagtcaatcg aataaccgtc
     9121 aagttcggcg gtctggttgc ggtcagcgag atcgacctca cggtggagaa gggagaaatc
     9181 cacgccctca tcgggccgaa cggagccgga aagacaaccc ttttcaatgt catcactcgt
     9241 gtcctgccgc cgacttcggg agagatctct ctaaacggaa agaacctgct caaattgcag
     9301 ccgcatcaaa cggttcaaat cggcatctct cgtaccttcc aaaatctgga gatcttccga
     9361 atgatgacgg tgaaagagaa cctgttcgtc ggataccaca atcgggtgaa tacgcgcatc
     9421 cttcaggagg ccttccaaac caaaggcttg cgtgccgaag aagagaaggt gcaagagaag
     9481 gcgatcgccg tttcggattt tttgggactc aaaaaccgtc tgaacagttt tgccggtcag
     9541 ttgccttatg gtctccaaaa aatggtcgaa atcgggcggg cgctgatgat cgacccggac
     9601 attctcttgc tcgacgaacc cgccgcggga ttgaacccgt ccgaaactca ggagctcaaa
     9661 cagatcatcc ggagtatccg gaaaaaaggc atcacgattc tcttagtgga gcacgacatg
     9721 tccttggtga tggatatctc cgatcgcatt acggtgatga acttcggaaa aaagatcgct
     9781 gagggagacc ctcgagagat acgaaatgac ccggtcgtta tcgaggccta tctgggggag
     9841 agtaagtatg cttgaactga aaggggtaaa gctaaaatac gggtacgtgg aagcgctcaa
     9901 ggggatcgac atgcgtgtgg aagagcatca gatcaccgct ttgctcgggg cgaacggggc
     9961 gggtaaatcc acgaccttac gcgcggtctc ctgtctcaac cgcatagaaa aaggggagat
    10021 cgtctttctc gacgaacaag tgcatcgcct gctgccttcg gagatcgtgc gcagaggaat
    10081 cgttcactgc cccgaagggc gaaagctctt tccctttctc accgttcaag agaacttgtt
    10141 ggccggagct tatacgcaga aagaccggaa tcaggtgcga aacttccaag agagggtgtt
    10201 tacgctcttt ccaatcctta aggaccgaag gagacaaatg gccggaaccc tctcgggagg
    10261 ggagcaacaa atgctcgcga tcggtagggc tttgatggcg gccccgaaaa tcctgatgct
    10321 agacgaaccc tccctcggat tggctcccaa agtagtggaa cagattttcg acacgttagt
    10381 caccttacgg gaagaaggta ccacgatctt tttggtggaa caaaacgcca atgccgcttt
    10441 gcatatcgcc gattacgcct atatactcga aatcggaaaa gtcgttctcg aaggcgaagg
    10501 aaaggccctg ttgaagaacg agaatgtcaa aaagaaatac ctgggaggat aggtgagcgc
    10561 cgtgctgtta caacaaatca tactgggatt gagggaagga agcctcttcg ccctcgtcgc
    10621 cctcggattg gtcatcgtcc ataaaaccac cggagtggtc aatttcgctt acgggcatat
    10681 gggtatgttc gccgcctaca tcaccttcac tttctacacc ctcctcgggt tcaacctcta
    10741 cctcgccgtg gcgatcggaa tcaccttcgc cgtctttttg ggaatcctca ccgaaaagtt
    10801 ccttcttcga ccgattcggc atctctctca ctctgcgatg ctgatcatta ccttagggat
    10861 gttgatgatc ctggaaggga tggcgctcct cttgtggaaa caagacttca agcagctgcc
    10921 caatctcatt accggtgtgc cataccggat cagcctgggc aactcctttc tcatcatcac
    10981 gaggcaggac gtgctgatct tcctcgtgac gaccgccatc gttttcggaa ccttcttcta
    11041 tctcaagtac accaaactgg gactggctgt gaggatggtc tcccagaacg aagaggcctc
    11101 gcatttgatg ggcgtcaaag tcggtcgcgt ctacggtttc gcctgggggc tcggtaccgg
    11161 gttgagcgcg ctcgctgcga tactggcggc tccgaaagag caggtccaac cgaatatgat
    11221 gctcaacctc cagattcaag gcctcaccgc cgccgtgttg ggagggtttg acagtttgcc
    11281 aggcgctatc gtgggcggcg tgctgttggg gatcatcgag cagctcgtag cgaagtatat
    11341 ctccgttgag ctcaaaacgg ccctcgcgct gatggtaatc atcatcttgc tcctcgtgaa
    11401 gcccgaaggc ttgctgggta agtccacgaa aaggagggtt tgatcatgaa cgccaaacgt
    11461 gattttttga ttttcgccgc cgttctgata gcgcttccct tgtttttcct gaaccaaacc
    11521 ttcctcttga gcatcttcgg tcaggtctta atcttcagca tcgccgctct gggattaaac
    11581 attttgatcg gcttcgcagg ccaaatctcg atcggacacg cctccttcat tgcggtcggc
    11641 gcctatttgt ccgcgattct aactaaaaac taccacatac cctttgcggt gaccgtcttt
    11701 ctatcaggcg gactggcggg gttgttcggc ctggtcctcg gcttccccgc tctacggttg
    11761 aaagggttct atttggcaat cgccacgatg gcctttgtgg ttatgatcga gcagctctta
    11821 ggctcttgga gttacgtggg cggtcacatc gggatgcgcg atattcccaa cttcgacctc
    11881 ttcggatacg tgttgaagtc tgatctgggc aagtattacg tgatcgcctt ctgtaccttc
    11941 ttcctattcc tcttcgggtt caatttcctg aaatccagga cgggaagagc attgaaagcc
    12001 atacgagaaa gcgagttcgc ggcgcggtcg gccggtgtca acgtcgcaaa atacaagttg
    12061 accgccttcg tcataagcgc gatttacgcc ggtatcgccg gcgcgttgta cgcccatacc
    12121 atcaactata tcgcgccgac ggacttcggg ttgggcgtct cgatcaacct gctggcgatg
    12181 attgtcgtcg ggggtttggc ctctttttcg ggaaatttca tcggcgccat cctcatgatc
    12241 gctatgccct tcctgttcag ccggtccaac ctcccgcttt cggtcatttt cgggatccta
    12301 ctcgtcatcg tcgtgctctt cttcccccgt ggcctaggat acggggtgca actgctcaat
    12361 ctcaaatact tcggtatccc ggccgcttgg ctgagaagga aaataagggt ttcatctgtc
    12421 gtaggaggta aagaaatgta cgtgaaccta cccgaaggaa agatgcatta tgaaatcggc
    12481 ggaaacccca caggcaaacc gttgtttatg gtacacggga acttcggttc ctggcgttgg
    12541 ttccagccgg tgatgtcccg attggaaaag acacctttta aaggggtggc catagacctc
    12601 ttgggattcg gagattccga aaaacccgaa cgcgagatct ctatccccca atatggacag
    12661 gagctggccg gcttcatcga cgtcttcaag gaagtaaaag caaacctgat cggccactca
    12721 ctagggggtg ccgtcatatg ggactacgcg gttaaccatc ccgacagggt gaaccgtatc
    12781 ctattgatcg atcccgcccc cgcggacggg ttagtcacac ccgaggagta ctaccccgcg
    12841 ctggcattgt accggaacaa ccgcgacctc ttgaaaaccg cgttggccgg aacgataccc
    12901 tcaggagacc ccgacaaact cctggataaa ctcgtcaacg acgcgatggt gatggacccg
    12961 cgcgccttta ccgaaaacgc ccgagccctg gagcgctaca attacctcgc ggatttacac
    13021 aagatccagt gccctgtgct catcgtctat ggagaaaagg atcttttaat caccgacaaa
    13081 ctgttggccg aaacactgaa gcgattaccg aacgccaggg tcaaagccct cccgggcata
    13141 ggccacagcg tcaacgtgga gaacccagac ctcgccgtac agatcatcaa cgaattcttc
    13201 ggaggctgat gggcgtgttc ttggcgttac gagacggcgg aagcctgtac tacgaaacct
    13261 tcggcgctaa aggggactgg atcgtctttc taaacggcat tatgatgagc gttccgagct
    13321 ggaaggactt tacggggcgg gtttcgaagc atttccggct attgctggtc gattttcggg
    13381 atcaagggca gtcgtccaag cgcgccgggc cctatcagtg tgcggttcat gtgcccgatc
    13441 tcgtggaact cttcgaccac ttgggaatcg aaagcgtgca tatgatgggc gtttcctatg
    13501 gcggacaggt cgctttggaa ttctgtcgag cgcaccagaa ccgtcttaaa accctcgagc
    13561 tggtgaccgt tttaccgaaa gtttcgaact atctggtcgc catgggggag ggatgggagg
    13621 tcgctgccgc gttaaacgac ggcgcgcggt ttttcagctt gacgatcccg agcatctatt
    13681 ccgacacctt ttacgcgcaa cgtctcgaat ggctaaaaaa cagacagaac gccttcagga
    13741 aaacactcac cccggaatgg ttcgaagcct tcatccgcct atctcagagc gctaaccggt
    13801 tcgattgcga agacgtcttg cccacgattc gcgttcccac actggtctta tgcgccgaaa
    13861 aggatatttt aacgccagcc aaggaaatga aagagatggc caaactcatc ccttcggcta
    13921 ccgttttaga gattccagac gccggtcacg cagcgttttt ggaaaaaccc gaggccttcc
    13981 taaccgccat catcggatac attaaaagca gggattgacc tgaacgctgc cattccggaa
    14041 gtacttgctt tatcgtccga ttaacaaaag aatcgctcgg aatgagcgat tcttttgttg
    14101 aggtttcgtt atcggatctc agtattgtag aatgcgtgga agcgccgcag cctgcttggt
    14161 ccaattttcc acctgtttgg cggtgggcag cttctgaacc aggtttttct gttcgttgac
    14221 ctccaccatc tttttgtaca ggttttccgg cttccgggct gccaattcgg gaacggtgtc
    14281 cactcccgcc aattccagca attcggagta gcggccgccg acgcctttaa tacgcattaa
    14341 atccgcgaaa tttgcccatt ttaacaagag tttttcggaa atgtccgcct tcttcgcaag
    14401 ttccgctctc cctttctttg aggtacaggc ttccagtaac tgttcaaccg acttgatccc
    14461 tacctctttg agtttgagtt cgtaggcttc gcctattcct tcgatgatac caagctttgt
    14521 catcgctttc cctcctttaa aatgtgtgaa aaacggttgt caggactgtc tacgccaata
    14581 gacgcgaccg ttggaatggt ttaacgtccc cagcgcgacc ggcgaccaat ttagtctatt
    14641 atatcactat catcgaaaga taacaaatac atgttttgac agatatgagt agtaatagag
    14701 aaatcatatt tctcatattt cgtgagcaaa tttcggaaga catcgaaaac cgtctctata
    14761 aaaagagcgc ggtaacgtgt ttgccggctt ttcctttttg ccgcgcagac catcataggt
    14821 tcgtgtcatc gatcacgtat tcggagtttt gattgtcctt ttggaggtct tcccgtaagc
    14881 gtcgtatggt ctcttccatt ttgaatgggg gaaggccagc gggatagccg agtgaaaaac
    14941 ggatcgaaga acgggagcgg cctcggatca cccgaaggtg ttcgatagat aggctgggat
    15001 cgatttttcc tgcgatttcg cataagtgcg cataaacgcg atcgacgatc tcggtgtgcg
    15061 tgcataccgg atcgacatgg atgacgattt cgataccga
//
LOCUS       DAOPXN010000045        15080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4021_length_15080_cov_5.704626, whole genome shotgun sequence.
ACCESSION   DAOPXN010000045 DAOPXN010000000
VERSION     DAOPXN010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4021_length_15080_cov_5.704626"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1438)
                     /gene="hutI"
                     /locus_tag="PKM49_03925"
     CDS             complement(206..1438)
                     /gene="hutI"
                     /locus_tag="PKM49_03925"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HNY81994.1"
                     /translation="MKTIVRNIRQLATPLGERLRKGAEMAVINEREHVDICLSAGRFL
                     RIGRDLNLEIKPEDTVIDASGLVATPAFVDPHTHIPFFGFREKEFFLRQRGAGYLEIL
                     QAGGGILETTRQVRAASLEDLIGFNLPFVYALQRKGVLVLEGKSGYGLDRENEWKQLE
                     ALSAIEKITGTKVFKTFMGPHAIPPEFPNADAAIDWLCAEVLEELAKNREMVDFLDVF
                     CEKGVFNYAQSERYLEKGKALGFKTRLHAEEMSFEGGSLLGAKVGASSVDHLIAIEPA
                     GIHALANSDTVATLLPGTSLFLNKPFAPARNLIDAGVGVALASDFNPGSCTFYDPTII
                     AFLAASKYKMTPAEILTAQTLNAASSLGVADSWGSIEEGKIGGLLLWDVPDFTAIPYL
                     PGHERLRFVIGPVGILRL"
     gene            1901..3544
                     /locus_tag="PKM49_03930"
     CDS             1901..3544
                     /locus_tag="PKM49_03930"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNY81995.1"
                     /translation="MKKVLWLFFLLSVVAISAFTCTTIAVTKGASVDGSVMVTHTADC
                     GSCDFRLVRIPAADHEPGSLRPVYPFIEEYPRYVGKDMGPGYDTPGFEPTKPLGYIEQ
                     VPHTYAYFDAVYGVMNEHQLSIGECTTAGNVYAMPKENVCIFDVAALSRVAMERCTKA
                     REAIQLMGELAVKYGYYGWGETLTVADTEEVWVFEITATPDQKSALWVAKKVPDGEVF
                     VESNEFRIREVLRNDPDMMYSPNLFAVCEAEKWWNPEKDKELDWLRTVSPGEYANPYY
                     SLRRVWSVLRRVAPSLNLSPYVEDTYTKAYPFSVKPDKKLSVADVFALHRDWYQGTEF
                     DLSKGLAAGPFGTPNRYGGASKLVKGAWERAVSVFRCDYSFVAQVRGWLPDPIGAVLW
                     FGPDAPHSTAYVPFYCNHIDLPEAYDSGSHAKFDPNNAFWVHSFAANWADLKFSYIIK
                     DIQAMQNKVEGRQFAQQPAIENAALQLYKVDPQLAIEFLTEYSNNNANRVVREWWEFG
                     QTLITRYNDGYVDGNSVGYPDWWLKEVGYESGPLKHGLK"
     gene            3566..4597
                     /locus_tag="PKM49_03935"
     CDS             3566..4597
                     /locus_tag="PKM49_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY81996.1"
                     /translation="MRNRFLWLFLLLSLTCFSVSILDFEAQSAFTVYVPGESLGLADV
                     NLFYFDRPTLEQNPEWFGKNLREDAIGAILLLSQKYGAGSDTRPVSFLYDITPVEGVE
                     KDTGYPLAAYLRFFTRGEEFGGESDCGDCGKPLAQGEKTLFLADFVKPLGLEGLWIEL
                     FNDRIDPYQQHTFTVDWFVNDQRIQKVVDVDPNQTSVSVCFWDPKLIMQIAQAFYEVI
                     CSEIVGPLSLRVSPVDSLERSILVSVDGVKLNSDVRNQPEELLAFNRQFLLGQKLLVS
                     FPDFIDQEDLKKYLAGAEPVPVLVWKSEPISGLPQVLWNIVLISNGFASIDGNNLILA
                     PFLRICKCQ"
     gene            4620..6266
                     /locus_tag="PKM49_03940"
     CDS             4620..6266
                     /locus_tag="PKM49_03940"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015335764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNY81997.1"
                     /translation="MKNRILVLLLLVSAVLTVFACTTIGVTKGASVDGSTMVTHTCDC
                     GMCDFRIIRTPAMDHPVGSMRPVYVYMDQYPAYVGLDRGPGWNTPGYEPTKPLGYIPQ
                     VPHTYAYFGSSYGIMNEHQLAIGECTTGAKIYAMEKENECIFDISALSRVALERCKGA
                     EEAVLLMGELGQKYGYYGWGETLVVADTEEVWVIEMCGTPDGKSALWAAQRVPDGEVF
                     VEANHFRIRDLEEKGQKFHYSSNLKEVAQKEGWWAPGQPLDWTKIVGSGEYGNAYYSH
                     RRVWRTLDRLAPSLGLSPWVEDTYTTAYPFSVKPEKKLTVADVIDLQRDWYQGTEFDL
                     SKGLAAGPFGNINRYAGSSKLVKGGWERAISVFRCAYVFVSQSRGWLPDPIGGLLWFG
                     PDAPHTSAYVPFYCGMTELPRVYEIGDLHTYDYESAGWTFNLMGNWMDLKFSYMIKDV
                     QAKQHEIEDREFAFQPAIEDAAKQLYLVSPELAVDFITDYAVNNANRVVQEWKRFTEK
                     MFAKYIDGYVDGNSVGYPDWWLQKVEFVTGQLAGQYAPNK"
     gene            6347..6979
                     /locus_tag="PKM49_03945"
     CDS             6347..6979
                     /locus_tag="PKM49_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNY81998.1"
                     /translation="MKKIVFLIAILGILVALPVFGKTVLGFPVVMTSAGQSDEINTVI
                     YLADEVGLKYDYCDILSLAEFKAGVGLAGAKNAPGKHVAYFSAEPKGTQFKTFLIAIG
                     ASLKGMGASGLSVDDEVNRIKALIAYAKESGMKILGMAIGGEIRRGLPGSLNEIMIDI
                     VAEHCDVIVVTSDTNMDKRFTNIAKEKNIEFVEVESAFDLLEAIPALFGL"
     gene            7019..8317
                     /locus_tag="PKM49_03950"
     CDS             7019..8317
                     /locus_tag="PKM49_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HNY81999.1"
                     /translation="MFNDPQFFQTIGYTLAMAGGYIVGKIFKLSTEICLFIAALVGAL
                     VAGSGLDVFRHFAEGSVTYFDIGLIFIFATLFMNILKESGAMDLIVRWIIRAFGNKKV
                     FFLILLMIVMLIPGALTGAGSISVLVVGGTVSAALSAMGLKKERVAAIVFIVAGLSAA
                     APPINVWAMMTCAGTAVPYVGFTMPLLVPILILGLFTVLFLGWKGKISNIEEALKTIP
                     EPKGLSVWSVAIPFIVLVALLSLPRIFPFDFPLLGLPLSFALSALVAWIMNYKRVKVF
                     RVMADTVAQLTPLIATTTVVGMFVQVMSFNGTKGLFSLWIVTAPMALLYIILPFVIPI
                     SEGLLTYGGVAVLGIPLIWMFNARGINTVIALSGLTLLWPLGDGLPPTKLIGRLTVDT
                     VGYTGSYGKFLKECIVPWIAITVVGILMVAFSNQLSFLMG"
     gene            8327..8479
                     /locus_tag="PKM49_03955"
     CDS             8327..8479
                     /locus_tag="PKM49_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82000.1"
                     /translation="MDFIMFLYYVMSAIIAIVFFWNYFKTKDKQKQILIAIALLPFLL
                     RLLRWK"
     gene            8488..9639
                     /locus_tag="PKM49_03960"
     CDS             8488..9639
                     /locus_tag="PKM49_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNY82001.1"
                     /translation="MKWSKWIILAILLLFLLAGGIPLFIQRNYKETLVMADGVTRVLR
                     LSDFFEPLKGTMGDTNIYEFQGKDPGGKVLILAGTHESEVSAMVTAFMFVENLQVQAG
                     TVYVVPHYNYSGSLGSQPGGGFPLYYTIPTPWGEKKFRLGDRGLQALDQWPDPDVYVH
                     YPDGQLLSYIDARNTNRTWPGKPNGTLGEKVAYAAMEMIRNEKIDIVIDLHEAEAMFP
                     VTNCIVAPEKSKSYAIGAALYVKGREKFESHVEPSPSNFRGLSHREVGDFSDALVFLL
                     EAPCVHLDQISGPKTNDLMIVGKDPFINVAAEKGLLFVPIDDRGWTIERRVGQQSSVI
                     QEIVNQFNKKNPARAILFTAPKHAEVVEKRMGAFFKDPDKAPKSRIAYE"
     gene            9743..11788
                     /locus_tag="PKM49_03965"
     CDS             9743..11788
                     /locus_tag="PKM49_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNY82002.1"
                     /translation="MKKTLFSVLLVLLASFSILIASGFFVDLDDNPYRNAIEGLFELT
                     WLGGDYGEEFLPKEPLTRGQAAHWLSEALKLPRIEPLDIDHPVEKSYTYKDPLGVIDE
                     SFVVSTYKDMDYHYYREEVEGLSRARILPITETFDVDDPISQEEFLIWLDNAIFGVDE
                     KYDNTRAERFHQLGILGMPKNPSSEPLTRELAACMLYSIIGDPKFQVVTVLVSADIHG
                     HLEPYKPVGSDYYIGGMAKMSRYVNQVWYDQKALLLLDVGDAPYNTNVANLFEGEPVI
                     RIMNWMGYDAMVLGNHDFDFPFSVLERNAKLADFPFLSANTLKDGRHPDFLKPYHIIE
                     CGGITFGIIGVTDDESAWYTHPNNVKGITFIDHFEAAKEAVAQLKGKVDFIIALAHLH
                     GDNHALSQKVEGIDLVYGGGTDIVAFPEKIHDSWLLSSGKHAELISQTNLNFWDKEVL
                     GFTFSHIFLSQNMPDDPVVDLIVKKAVSAMDARLMRVVGETKVLLDGERSTVRLKESN
                     LGNVIADSLRAMTDADIALQNGGGIRESVPPGKITVKDIYTVLPFDNTVVVVEATGKQ
                     VWDALEHGIAAYPGAAGQFLQVSGVSYTFDASKEPYHRLLSVIYNGAPIEMDRKYRVV
                     ANDFLTGGGDKFLMLTECKELVRTKSYLRDAFTEYFLKNPVISPQLEGRITILNPAN"
     gene            12076..14181
                     /locus_tag="PKM49_03970"
     CDS             12076..14181
                     /locus_tag="PKM49_03970"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HNY82003.1"
                     /translation="MAKTLVLAEKPSVARDIARVMGCSQKGNGFLEGSRYVVTWALGH
                     LVTLADPDAYGPQYKTWKMEDLPLLPTEMKLVVIKETGRQFQVVKTQMMRGDVSEIVI
                     ATDAGREGELVARWIIEKVHVRKPIKRLWISSVTDRAIQEGFTRLRDGRDYENLYHSA
                     VARSEADWYVGINATRALTCKFNAQLSCGRVQSPTLAMIAHREEEIRHFVPKSYYGIS
                     AKTHGVTLIWRDKKTKEYRTFDEAYRLSVLKGLTDKTATVVGVEKTEKKTFPPLLYNL
                     TELQKDANARFSFSAKDTLSIMQSLYETHKLLTYPRTDSRYLSADIVDTIRERINACG
                     VGIYAPLGARVRNLCIGSNAFFVDDTKVTDHHAIIPTEQKVYLDKLSLEERKIYDLVI
                     RRFFAVLLPPFEYEQTAVEARINGETFIARGKRVLKAGWKEIYGHFDDEDPDEEGLAV
                     QILPEIRKGDRLTLDAIFETTGTTKPPARFTEATLLAAMENPARWLEGEKKDLAKTLG
                     ETGGLGTVATRADIIEKLINTFLIEKEGRELPITSKGEQLLNLVPKELKSPALTAVWE
                     QKLALIAKGTLNKDSFVADMKRYASEIVKEIKNSEAVFKHQNLSGKRCPTCGKNLLLV
                     KDKRGSLYLCEDRVCGYRERILSPKKVAFEDRKTEKQGGLSKKEIRQYLQSQEKSDNQ
                     PHNTALAEALKRFQESEKP"
     gene            14325..>15080
                     /locus_tag="PKM49_03975"
     CDS             14325..>15080
                     /locus_tag="PKM49_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HNY82004.1"
                     /translation="MATKKVLIIGAGIAGLSAASYLQRNGYSTEIFEAHYLPGGLCTA
                     WKRGQYTFDYCIHWLVGTNPETGFDVLWNELGAFQNEDGSKTQIRNFDESSRVELSDG
                     QIVRFYSNADRLKEELLRVGPDDGKEIERLTNDLKTLSRLIPVADPEKRSVMYRLAVN
                     LRYLSIMSRFMRHLKTPMQDYARKYHSEAIRKALLAVTPAEWSLATLTMGLGMQHSQA
                     AGYPVGGSLNLARNIERKYLSLAVKSITVTQWKK"
BASE COUNT         3717 a         3826 c         4031 g         3506 t
ORIGIN      
        1 ccggtacaaa tctacgaggg ggtggcgcgg ttttcaaagc gatacccggc tccttcaacg
       61 acccaggcaa ctcgggatcc tcgatccaat acgcggggat acagtaattt agcctcaaga
      121 gaaaaaaaca tgccgaacta aagttcggcg ttcccaggac gctcccataa agaaatgaag
      181 cgccccggtg agaccggggc gctttttata ggcgtagaat acctacgggg cctatgacga
      241 accgtaggcg ctcgtgcccg ggaaggtaag ggatggcggt gaaatcggga acatcccaaa
      301 gcaagaggcc tcctattttt ccctcctcga tcgatcccca ggaatccgcg acgcctaacg
      361 aggaggccgc gttcagggtt tgagcggtga ggatttcggc aggggtcatc ttgtacttgg
      421 aagccgctag aaaggcgatg atcgtggggt cgtaaaaggt gcaagacccc ggattgaagt
      481 cgctggccaa cgcgacgccg accccggcat ctatcagatt cctggcgggc gcgaagggct
      541 tgttgaggaa caggctggtg cccggtaaca gggtggcgac agtgtcgcta ttcgccaacg
      601 cgtgtatacc ggccggttcg atcgcgatca ggtggtcgac ggaactggct ccgactttgg
      661 cacccagaag gctgccgccc tcgaaactca tctcctcggc gtgcaatctc gttttaaaac
      721 ccaatgcctt gcccttctcg aggtatctct cggattgggc gtagttgaaa actccctttt
      781 cgcaaaacac gtctagaaag tcgaccatct ctctgttttt ggccaattcc tccaagacct
      841 ccgcgcacag ccaatcgatg gcggcgtccg cgttcggaaa ctcgggagga atggcgtgcg
      901 gacccataaa ggttttgaag actttcgtcc ccgtgatctt ttcgatggct gatagggcct
      961 ccagttgctt ccattcgttc tctcggtcga gtccgtaccc gctctttcct tccagcacga
     1021 ggacgccttt cctttgtagg gcataaacga aagggaggtt gaaccctatc aggtcctcta
     1081 gcgacgccgc tctgacctgt cgggtcgttt ctaaaatccc gccgccagcc tggaggatct
     1141 ccagataacc ggcccccctt tggcgtaaga agaactcttt ttctcgaaat ccaaagaacg
     1201 ggatgtgggt atgggggtct acgaaggcgg gcgtcgcgac caacccagac gcgtcgatga
     1261 ccgtgtcttc gggttttatt tcgagattca aatccctgcc gatccggaga aagcggccgg
     1321 cagagagaca gatatcaacg tgttcacgtt cgtttatgac ggccatttcc gccccttttc
     1381 gaaggcgctc ccccaggggc gtagccaatt gacggatgtt ccggactatc gttttcatct
     1441 tgctcgcctc ttttcgctat ttattgacgt ctaaattata acggattcga gcctaattat
     1501 caaagttacg gtctctcacg gtcagatacg atcttaagga tttttatcga ctccattcac
     1561 ggtttcttcg ccggatttca tacataattc ctcatttgcg tgaaggtaat ttgaattttt
     1621 tatgtgtgtc aatatattat tttctaaata ttattcgcga tcacgcgttt gctcatagac
     1681 cagatggtca ttagttgatt ttatagactt ttattatcga atattggaac agaataatta
     1741 ttaatgtatg accttctatc aatgacaaac ggtgtagcat cggacgtgaa ttaatcatgt
     1801 aaacaacgag attatttcgg agatatgaaa agatttcgtt ttccgaacgg ttattgtata
     1861 ataggggtgc ttctcactaa tcttcaaagg ggtgatccat gtgaaaaagg ttctttggtt
     1921 attcttcctg ctcagtgttg tcgccatcag tgcttttact tgtactacaa tcgccgtgac
     1981 caaaggcgcc tccgtggatg ggtcggttat ggttacccac actgcggatt gcggatcctg
     2041 tgacttccga ctggtcagga ttccggcggc cgatcacgag cccggttcac tgaggcccgt
     2101 ttacccgttt attgaagagt atccgcgcta tgtcgggaaa gacatgggac cgggctatga
     2161 cacgccgggg ttcgaaccca caaaaccgct cggatacatt gagcaagttc ctcacaccta
     2221 tgcgtacttt gacgccgttt acggagtgat gaacgagcat cagctgtcga tcggcgaatg
     2281 tacgacggcc ggaaacgtgt atgccatgcc gaaagagaac gtgtgcattt tcgacgtggc
     2341 cgccttgtcc cgcgtggcga tggaacgctg caccaaggcc cgcgaagcta tccagctgat
     2401 gggtgagttg gcggtgaagt acgggtatta cggatgggga gagaccctca ccgtagccga
     2461 taccgaagag gtatgggtat ttgagattac cgcaacgccg gatcagaaga gcgcgttgtg
     2521 ggtagcgaaa aaagttccgg atggagaggt attcgtggaa tcgaacgagt tccgcattcg
     2581 ggaagtgttg agaaacgacc ccgatatgat gtattccccg aacctgttcg ccgtttgtga
     2641 agcggaaaaa tggtggaacc ccgaaaaaga caaagagctg gattggctga gaacggtcag
     2701 ccccggcgag tacgcaaatc cgtactacag cttgagaagg gtatggagcg tgttaagacg
     2761 ggtggctccg tccttgaacc tgagccccta tgttgaagac acttacacca aagcctaccc
     2821 gttctccgtc aaacccgaca agaagttgtc cgtagccgac gtcttcgcgt tacatcgcga
     2881 ttggtatcag ggaactgagt tcgacttatc caaaggcttg gccgcgggtc catttgggac
     2941 tcccaaccgc tacggcggcg cgagcaagtt ggtgaaaggc gcctgggaaa gagcggtatc
     3001 cgttttcaga tgcgattaca gctttgtggc gcaagtgcgc ggctggctac ccgacccgat
     3061 cggtgccgtg ctgtggttcg ggcccgacgc gcctcatagc actgcttatg ttcccttcta
     3121 ttgtaaccac atcgatctgc ctgaagccta cgattccggt tctcacgcaa aattcgaccc
     3181 caacaacgct ttctgggtcc attcttttgc ggcgaactgg gctgacctga agtttagcta
     3241 tatcatcaaa gacatccaag cgatgcaaaa caaagtggag gggcgtcaat tcgcccaaca
     3301 gcctgcgata gagaacgctg cgctgcaact ttacaaagtc gatccacaac tcgctatcga
     3361 atttttgacc gagtacagca acaacaacgc caaccgtgtc gtgagagaat ggtgggagtt
     3421 cggtcaaacc ctcatcaccc gctacaacga cggctacgtg gacggcaatt ccgtgggtta
     3481 ccccgactgg tggctcaaag aagtcggcta cgaaagcggt ccgctcaaac acggattgaa
     3541 gtaatggttt aacggaggag gatatatgcg aaatcgtttt ctatggctct ttttgcttct
     3601 gtcgttgacc tgcttttccg tatcgatttt ggactttgaa gcgcaatcgg ccttcacggt
     3661 ctatgtgccg ggagagtctc tgggactcgc cgatgtgaac ctcttctact tcgaccggcc
     3721 gactttggaa cagaatccgg agtggttcgg caagaacttg cgagaagatg ctataggagc
     3781 gattttactg ttatcccaaa agtacggggc tgggtctgat acccgccccg tatccttctt
     3841 atacgacatc accccggtcg aaggggtaga gaaggatacc ggctatcctc tcgcagccta
     3901 tctaaggttt tttacgagag gtgaagagtt tggcggcgaa agcgattgcg gtgactgtgg
     3961 aaaaccgctc gcgcaagggg aaaaaactct cttcttggcc gatttcgtta aacccttagg
     4021 gctcgaaggt ctttggatcg agttattcaa tgacaggatc gatccctacc agcagcatac
     4081 ctttacggta gattggttcg tcaacgacca aaggatccag aaggtcgtgg acgttgaccc
     4141 aaaccaaacg tcagtttccg tctgcttctg ggatcccaaa ctcatcatgc aaatcgcgca
     4201 ggccttttat gaagtgatct gttccgaaat cgtcggcccg ctgtctctaa gagttagccc
     4261 ggttgactcg ctcgagcggt cgatcctcgt ctcggtggac ggggtgaaac tcaacagtga
     4321 cgtacgcaat caaccagagg aacttttggc tttcaacaga caatttttgc tcggtcaaaa
     4381 gttgctcgtc tctttcccag attttatcga ccaagaggac ctgaaaaaat atttggccgg
     4441 cgctgagcct gttccggttc tcgtatggaa gagcgaaccg atctccggct taccacaagt
     4501 gttatggaat atcgttctca tctctaacgg ctttgcttct atcgatggta ataatctcat
     4561 actggcacct ttcttgcgga tatgcaagtg tcagtaaaaa ctcaaaagga gtgatgtatg
     4621 tgaaaaacag gattttggtg cttttgctcc tcgtcagcgc agtcttgacc gttttcgcct
     4681 gtacgaccat cggtgtcaca aaaggcgcgt cggtggacgg ttccaccatg gtcacccaca
     4741 cctgcgactg tgggatgtgc gatttccgta ttattcgaac ccccgctatg gatcacccgg
     4801 tagggtcgat gagacctgtc tatgtttata tggatcagta tccggcctat gtgggtctag
     4861 accgcggccc cggttggaat accccgggat acgaaccgac gaaacccctc ggttatatcc
     4921 cacaagtccc gcatacctac gcgtacttcg gttcgtccta cgggattatg aacgagcacc
     4981 agctggcgat cggcgagtgt acgaccggcg cgaagattta tgcaatggaa aaagaaaacg
     5041 agtgtatttt cgacatttcg gcgctttcca gagtcgcgct cgaacgctgt aaaggtgccg
     5101 aagaagccgt cctgttgatg ggcgagctcg gtcaaaagta cggctattat ggctggggag
     5161 agaccctcgt cgtcgctgac acagaagaag tatgggtcat cgagatgtgt gggacgccgg
     5221 acggaaagag cgcgctttgg gcggcgcaac gcgtccccga tggcgaagtc ttcgtagaag
     5281 ccaaccactt cagaattcga gatttggaag aaaaaggaca aaagttccac tattcctcca
     5341 acctgaagga agtcgcgcaa aaagagggct ggtgggcgcc cggacaaccg ctggactgga
     5401 ccaaaatcgt cggcagcggc gaatacggta acgcctatta cagccacaga cgcgtatgga
     5461 gaaccctcga ccgcctcgcg ccgtccctag gcttgagccc ctgggtcgaa gacacttaca
     5521 ccaccgccta cccgttctcc gtgaaacccg aaaagaaatt gaccgtggcc gatgtcatcg
     5581 acctgcagag agactggtat caagggaccg agttcgacct ttccaaaggg ttagcggccg
     5641 gcccgttcgg caacatcaac cgatacgccg gaagcagcaa attggttaaa ggcggttggg
     5701 aaagagccat ttccgtgttc agatgcgctt acgtgttcgt ctcccaatcc agaggttggc
     5761 tccccgatcc gatcggagga ctcctatggt tcgggcctga tgcgccgcac acctccgctt
     5821 acgtaccctt ctactgcggt atgaccgaac tcccgagagt ttacgagatc ggcgacctcc
     5881 acacctacga ctacgaaagc gcgggttgga ctttcaactt gatgggtaac tggatggatc
     5941 tcaaattcag ctacatgatc aaggacgtcc aagcgaaaca acacgagatt gaagacagag
     6001 aattcgcttt ccaaccggcg atagaggatg cggcgaagca gctctatctc gtttcaccgg
     6061 aactcgcggt cgatttcatc accgactacg cggtgaacaa cgccaaccgc gtggtgcaag
     6121 aatggaaacg ctttaccgaa aagatgttcg cgaagtacat cgacggttac gtcgatggta
     6181 attctgttgg atacccggat tggtggctac agaaagtcga attcgtcaca ggccagctag
     6241 ccggacagta cgcgccgaat aaataatcat tcgatccatt ctcaggaccc cgacgcggcg
     6301 tcggggtcct tactcggaaa tataagaatt gaaggaggtt aaaaagatga aaaaaatcgt
     6361 gtttcttatt gccatcctcg gaatactggt cgctctaccc gtctttggaa agaccgttct
     6421 cggattccca gtcgtgatga cttcggccgg acaaagcgac gaaatcaaca ccgtgatcta
     6481 tctggccgac gaagtgggat tgaagtacga ttactgtgac atcttgtctt tagccgagtt
     6541 caaagccgga gtgggtttag cgggagcgaa aaatgccccc ggaaaacacg tcgcctattt
     6601 ttcggccgaa ccgaaaggca cgcagtttaa aacattcttg atcgcaattg gtgccagttt
     6661 gaaaggtatg ggagcctccg gtttgtccgt agatgacgaa gtcaatcgca tcaaggcctt
     6721 gatcgcctac gcgaaggaga gcggaatgaa aattcttggg atggccatcg gaggggaaat
     6781 ccgcaggggg ctacccggga gccttaacga gattatgatc gatatcgtcg cggagcactg
     6841 cgatgtcatc gtcgtgacca gcgataccaa catggacaaa cggttcacga atatcgcgaa
     6901 agagaagaac atcgaattcg ttgaggttga gagcgcattc gacttactcg aagctatccc
     6961 tgccctgttt ggattgtaat cgtctctcat tttctcaact ctctaagggg gtttcaacgt
     7021 gtttaacgat cctcagttct tccaaacgat cggctatact ctggcaatgg cgggcgggta
     7081 tatcgttggg aagatcttta aactgtctac agagatctgt ctgttcatag ccgccctcgt
     7141 aggtgcgttg gtagcaggct caggactaga cgtattccgc catttcgccg agggttcggt
     7201 gacctatttt gacatcggtt tgatttttat tttcgcgaca ctgtttatga atattcttaa
     7261 ggagtccggc gcgatggact tgatcgtccg ctggatcatc cgcgccttcg ggaataagaa
     7321 ggtcttcttt ttaatcctgc tcatgatcgt tatgctcata cccggcgcgt tgacaggggc
     7381 cggaagcatc tcggttctcg tcgtcggcgg aaccgtgtcc gcagccttaa gcgcgatggg
     7441 actgaagaag gaacgcgtgg ccgcgatcgt cttcatcgtg gccggactca gcgcagccgc
     7501 acctcctatc aacgtatggg cgatgatgac ctgtgccggg acagccgtgc cgtacgtcgg
     7561 attcactatg cctttactcg tcccgatcct catcttgggc ttgttcaccg tgttgttcct
     7621 cgggtggaaa ggaaagatat cgaacataga agaagccctc aaaacgattc ctgagcccaa
     7681 gggactgagc gtttggagcg tagcgattcc gtttatcgtt ttggttgcgt tgctgtcgct
     7741 gccacggata ttcccgttcg atttcccact gctgggatta cccctgtctt ttgcgctcag
     7801 cgcgcttgtc gcttggatta tgaactacaa gcgagtgaag gttttccgag ttatggctga
     7861 taccgtcgct cagctcaccc cgttgatcgc gacaaccacc gtcgtcggta tgttcgtcca
     7921 agtgatgagc tttaacggca ccaaagggct attctcgctt tggatcgtca cggcgccgat
     7981 ggcgttgctc tacatcattc tcccatttgt gattcccatc tcagaaggat tgttgaccta
     8041 cggtggggta gccgttttgg gtatcccgct gatatggatg ttcaatgcca gagggatcaa
     8101 caccgtgatc gcgctgtccg gcttgacact tctgtggccg cttggagacg gattgcctcc
     8161 cacaaaactg atcggaaggc tcacggtcga taccgtcggc tacacaggca gttatggaaa
     8221 gttcctgaaa gaatgcatcg ttccttggat cgccatcacg gtcgtcggta tcctgatggt
     8281 cgctttcagt aatcaacttt ccttcctcat gggttaggtg gttagtatgg attttattat
     8341 gtttttatat tacgtgatgt cggcgatcat cgcgatcgtc tttttctgga attattttaa
     8401 aacgaaagac aaacaaaaac aaatcttaat agcgatcgca ttgctgccat tcctactacg
     8461 attgctccga tggaagtagg tgcgaagatg aaatggtcta aatggataat actggcgatt
     8521 ctgcttcttt ttctcttggc cggtgggata ccgctcttta tacaaaggaa ctacaaagag
     8581 accttggtga tggcggatgg cgtaactcgg gtcctgaggt tgagcgattt ctttgagcca
     8641 ttaaaaggga cgatgggcga cacgaatatt tacgaatttc aaggcaagga ccccggcggg
     8701 aaagttctga tccttgctgg cactcacgaa agcgaagttt ctgcgatggt cacggcgttt
     8761 atgttcgtgg aaaaccttca ggttcaagcg ggtaccgttt acgtcgtacc gcattacaac
     8821 tacagcggct ctttgggctc ccaacccggc ggcggctttc ctctgtacta cactatcccc
     8881 acaccttggg gagagaaaaa attccgctta ggggatagag gactacaagc gctagaccaa
     8941 tggcccgacc cagatgtgta cgtccactac cccgacgggc agttgttgtc atacatagac
     9001 gcaagaaaca cgaacaggac ctggcctggc aaaccaaacg gcacgttggg agaaaaggtc
     9061 gcctacgccg ctatggaaat gatccgcaat gaaaagatcg atatcgtgat cgacctgcac
     9121 gaagcggagg cgatgttccc tgtcaccaat tgtatcgtcg ctccggaaaa aagtaagagc
     9181 tatgcgatcg gcgccgcgct gtacgtgaag ggtagagaga agttcgaaag ccacgtagag
     9241 ccgtcgccct cgaacttccg cggcctttcg catcgcgaag tgggagattt ttccgacgcc
     9301 ctcgtctttt tgttggaagc cccgtgtgtt cacctggacc agatctcggg acctaagacc
     9361 aacgatttga tgatcgtcgg aaaagacccg tttatcaacg ttgccgcgga gaagggcttg
     9421 ctgttcgtgc cgatcgacga ccgcggttgg acgatcgaga gacgtgtagg acagcaatct
     9481 tcggttattc aagagatcgt caatcaattc aacaaaaaaa acccggcgcg ggctattctg
     9541 tttactgctc ctaaacacgc tgaagtggtt gaaaagcgta tgggggcctt ctttaaagac
     9601 cccgacaaag cgccaaaatc tcgaatcgct tacgaataag tagaaagggc aaacgaaaga
     9661 gtttgccctt tttttcgcta ggacaggaaa gccggtttac cgaaaccccg aaaacctttg
     9721 aaaacgaaag gagaggatcg taatgaaaaa aactttgttc agcgttcttc tggttctttt
     9781 agccagcttc tctatcctca tcgcgagcgg tttcttcgtc gatttggatg acaaccccta
     9841 ccgaaatgcc atagaggggc tctttgagct cacgtggctc ggtggcgatt acggagagga
     9901 gtttttaccc aaggaacctt tgacgagggg acaggcggct cattggctga gcgaggcgct
     9961 gaaactcccg agaatcgaac cgctcgatat cgatcacccc gtcgaaaagt cctacaccta
    10021 taaggatcct ctgggtgtga tcgacgagtc cttcgtcgtc tccacatata aggacatgga
    10081 ttaccattac tacagagagg aagtggaagg cctttctcgg gccagaatct tgcccatcac
    10141 tgaaaccttt gacgtggacg accccatctc tcaagaagaa tttctcatct ggctggacaa
    10201 cgcgattttc ggtgtggatg aaaagtacga taacacgcgt gcggaacggt ttcatcaatt
    10261 gggcatcttg ggaatgccaa agaacccctc ttcagaaccg ctcactcgag aattggcagc
    10321 ctgtatgctt tatagtatca ttggagaccc gaaattccaa gtggtgacgg ttttggtgtc
    10381 cgcagatatc cacggccacc tcgaacccta caaacccgtc ggctccgatt attacatcgg
    10441 cggtatggcg aagatgagcc ggtacgtgaa tcaggtgtgg tacgaccaaa aagcgttgct
    10501 attgctggac gtgggtgacg ccccttacaa cacgaacgtc gccaacctct tcgaaggcga
    10561 gccggttatt cgtattatga actggatggg gtacgacgcg atggtactcg gaaatcacga
    10621 ttttgatttc cccttctcgg ttttggaacg gaacgccaag ctggccgatt tcccgtttct
    10681 ttccgccaat acgctgaagg acggccgtca tcccgacttc ctgaaaccct accatatcat
    10741 agaatgtgga gggatcacct ttggaatcat cggggttacg gacgatgaga gcgcgtggta
    10801 cacgcatccg aacaacgtga aagggatcac gttcatcgat cacttcgagg cggctaaaga
    10861 agcggtagct caactaaaag gaaaagtcga ttttatcatc gccttagcgc atttgcacgg
    10921 cgacaatcac gcgcttagcc aaaaagtcga aggcatcgac ctggtctatg gcggtggcac
    10981 cgacatcgtc gccttcccag aaaagattca tgattcgtgg ctgctgtcct cggggaagca
    11041 tgcggaactg attagccaaa cgaacctcaa cttctgggat aaagaggtcc tcggattcac
    11101 cttctcgcac atcttcctct ctcaaaatat gcccgatgat ccggtggtgg atctcatcgt
    11161 caaaaaagcg gtgtccgcga tggatgcccg gctgatgcgc gtcgttggcg aaacgaaggt
    11221 gttgctggac ggcgagagat cgaccgttcg tttgaaagaa tccaaccttg ggaacgtcat
    11281 cgcggacagt ttaagggcga tgaccgatgc cgatatcgcc ttacaaaacg gcggaggcat
    11341 acgcgagagc gttcctcccg gaaagatcac ggtcaaagat atctacacgg ttctcccgtt
    11401 cgataacacc gtcgtcgttg tcgaagcgac gggtaagcaa gtctgggatg ccctcgaaca
    11461 cggaatcgca gcctacccgg gagcggccgg ccaattcctg caagtctccg gcgtcagcta
    11521 cacgttcgac gcctcgaaag aaccttacca ccggctcctc tccgtcatct acaacggcgc
    11581 gcctatagag atggacagaa agtatcgcgt cgtcgccaac gatttcttga caggcggcgg
    11641 agataaattt cttatgctga cagagtgtaa agagttggtc cgaacgaagt cctatctacg
    11701 tgacgcgttc accgagtact tcctgaagaa cccggtgatc agcccgcagt tagagggcag
    11761 aatcacgatc ctgaacccgg caaactagcc ggcttgatta cgggccctag aaccgtggcg
    11821 aagagcggac gaaaaaggtc cgctcttttc ttgcgaacgc catcgcgttt tcagccttca
    11881 agaaacctgg ggtacgtcac atgaagagtt tcccgtaaaa cgcaacggtg ttcttcctga
    11941 ccaaaccttc cttttcctat ttaccagaat ccggtgaacg accattaaag ctaatttcct
    12001 gtttcgtatg ttatgatcga agggcgcgat acgtgcgcga agatactatc tccatataat
    12061 gaaaggaacg actcaatggc gaaaacactc gtgttagcgg aaaaaccttc ggtcgcgagg
    12121 gatatcgcgc gggttatggg ctgctctcaa aaaggcaacg gttttctcga aggatcgcga
    12181 tatgtggtca cttgggcgtt gggccactta gtcaccttgg cggacccaga tgcttacggc
    12241 ccccagtata agacgtggaa aatggaggac cttcctctgt tgcctacgga gatgaaattg
    12301 gtggtaatca aggaaaccgg gcgccagttc caggtcgtga aaactcagat gatgcgcgga
    12361 gacgtgagcg agatcgtcat cgcaaccgac gctgggcgtg agggagaact cgtcgcgcgt
    12421 tggattatcg agaaagtaca cgtgagaaag ccgatcaaac gcttgtggat ctcttcggtg
    12481 accgatcgtg cgatccagga gggttttaca cggttacgcg acggcagaga ctacgaaaac
    12541 ctgtatcatt cggcagtcgc gcgttcggaa gcggattggt atgtgggaat caacgccacc
    12601 cgggcgttga cctgcaagtt caacgcgcag ctttcctgcg gccgcgtcca gagcccaaca
    12661 ctcgctatga tcgcccatcg tgaagaggag atccggcact ttgttccgaa atcctattat
    12721 ggaatcagcg cgaaaacgca tggagtcact ttgatctggc gcgataaaaa aacaaaagaa
    12781 taccgtacgt ttgatgaagc gtatcgcctg tccgtgctga aagggttgac cgacaagacc
    12841 gccaccgtgg tgggggttga aaaaaccgaa aagaaaacct ttccgccctt gctatacaac
    12901 ctcaccgaac tccaaaaaga cgcaaacgcc cgtttttctt tttctgccaa agacacgcta
    12961 tcgattatgc agtcgcttta cgaaacccat aagttgctga cctatccgcg gacagactct
    13021 cgctacctat cggccgatat cgtcgacacg ataagagagc gtatcaacgc gtgcggcgtg
    13081 gggatatacg ccccgttagg cgccagagtg cgaaacctct gtatcgggtc gaacgccttc
    13141 ttcgtggatg ataccaaggt caccgatcac cacgcgatca tcccgacgga gcagaaggtc
    13201 tatctcgaca agctcagcct cgaagagcgc aagatctacg atctggtgat acgccgattc
    13261 ttcgccgtcc tgctacctcc attcgaatac gaacagacag cggtggaagc ccggatcaac
    13321 ggtgaaactt ttatcgcgag agggaaaagg gtcctgaaag ccggatggaa ggaaatctac
    13381 ggacatttcg atgatgagga tccagatgaa gaggggctcg cagttcaaat attgcctgaa
    13441 attcgtaagg gagaccgttt gacgctcgac gcgatctttg aaacgaccgg aaccaccaaa
    13501 ccgcccgcgc gatttacaga ggccacgctg cttgcggcta tggagaatcc cgcccgatgg
    13561 ttggaagggg aaaagaaaga ccttgcgaaa acactaggag aaaccggtgg gctgggaacg
    13621 gtcgcgacac gggcggatat catcgaaaaa ctcatcaata cctttctgat cgagaaggaa
    13681 ggcagagaat tgcccatcac ctccaagggg gagcaactgc tgaacttggt tccaaaagag
    13741 ttgaaatcgc ccgcgctcac cgctgtttgg gaacagaagc tggccttgat cgcaaaaggg
    13801 acgctgaata aggactcgtt cgtggcagat atgaaacgct atgcctcgga aatcgtcaaa
    13861 gagatcaaaa acagcgaggc ggtgttcaag catcagaacc tgtctgggaa acggtgtccc
    13921 acgtgtggca agaatttgct tctggtcaaa gacaaacggg gtagcctgta cctctgcgaa
    13981 gatcgggtgt gcggttaccg ggaacggatt ctcagcccaa aaaaagtggc cttcgaagac
    14041 cggaagaccg aaaaacaagg cggactgtcc aagaaagaaa tccgacaata cctgcagtcg
    14101 caggagaaat cggataatca gccacacaac accgctctgg ccgaagcgct taagcgattc
    14161 caggagtccg aaaaaccata aaaatttcct atgacggagt tcacctttag ttctgcggga
    14221 cattcccaag tgtatcccca cacacgcgag ccacgcacag aaagaaatat gataaactaa
    14281 cctcgaaaga gtcgtgttct ggataataaa aggagggggg cagtatggct acgaaaaaag
    14341 tgttgatcat cggcgccggt atcgccgggc tatcggccgc gtcttacctg cagcgaaatg
    14401 ggtattccac agagatattc gaggcccact accttccggg aggcctttgc accgcctgga
    14461 agcgcggaca gtatacgttc gattattgta ttcattggtt ggtcggcacg aatccggaaa
    14521 ccggtttcga tgtgctgtgg aatgaattgg gcgcttttca aaatgaagat ggcagcaaaa
    14581 cccagatacg gaatttcgat gagtcttcac gtgtggagct ttccgacggg caaatcgtcc
    14641 gtttttactc aaacgcggac cgtctgaaag aagagctgct tcgcgtcggc cccgatgacg
    14701 gtaaagaaat cgagcggtta accaacgatt taaaaacgct gagccgcctt attccggtcg
    14761 cggatcccga aaaaagaagc gttatgtatc gcttagcggt gaatcttcgg tacctttcaa
    14821 ttatgagtcg atttatgcgg cacctgaaaa ctcctatgca agactacgcg cggaaatatc
    14881 acagtgaggc catacggaag gctttgctcg cggtgacccc ggcggaatgg tctctcgcga
    14941 cgttgacgat ggggttgggc atgcagcaca gccaagccgc cggatatccc gtcggaggct
    15001 ccctcaatct agctcggaat atcgagcgga agtacctctc tctggcggta aaatccatta
    15061 ccgtcaccca gtggaaaaaa
//
LOCUS       DAOPXN010000046        15013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4053_length_15013_cov_5.177965, whole genome shotgun sequence.
ACCESSION   DAOPXN010000046 DAOPXN010000000
VERSION     DAOPXN010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 15013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15013
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4053_length_15013_cov_5.177965"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..4459
                     /locus_tag="PKM49_03980"
     CDS             206..4459
                     /locus_tag="PKM49_03980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82005.1"
                     /translation="MRIIKLLRSNYDGDEYLIEHEGTYKRSKHLSREAYGLSLDKLAD
                     RITAISKARSRFLYPDYVGLDVNPPVFHFPFHEESDVHNSILEEGHRLQIAQSLLENF
                     ELFQHASSIGFGVWGLPDLLISDRLYFLTPLWVNYRQNSLKNLLNRSTVVVASELLEG
                     KQPTPASDVYVLGRILEAILPERILTEVRDPVTQMTDPNPANRPTHYSRILSQPQFAG
                     LPKALSTAIKREFISPNIIEREKELAAFMSLLNERKHRGVSLIVIRGQTRVGKSSFLT
                     LAQNEMWNEGWKTIVASDLQRFAQELLQIVESPAKVGIDEEDFSYLWDLGDVYNFDRV
                     LSIVGKLLTSVDKLAILVDDIELADERYSRLLKSIRNMIVDNQIVIAMTQLREKGTID
                     YEHLIHLRPFTEEQTEKLLELMLGERFVKKHKDKVLWISKITGGYPGYIISLLNLLNG
                     SGKLFIEDGRWVLSEDMTSIDGFGDYISTMFPSLEPEEQSLLSKIACLSEKFTAEELD
                     ALGEAIDLPLEKLRKSFWHFQDLGLFFTEDHLFRFSLQEIWEQSYARCPQELRYRLHR
                     RFAEYGSQLNKQAWHYKEIGETRMAAKIYLCASRKAFRKNEGFETARGYMDEVFQLLK
                     PEEISPSMLGWYAILCAYDGKPFPESMEESLYNTENYRYLCIANRIIKGEYQKADEMC
                     GRQAGLFKDKKLSQALWVTQMIYACATLKVKIGELDNALSLLNLVLERVCNKKTDAHN
                     RLEIMAYNLIAQAYERKNNWQPAYSICEANIERAKKYHMSYLLPDVFLTAGNIVFPLG
                     PGYAQPHYENAIKIAKVFFSAQKSLQPTLELANGFLYSGETAKMFDYLEKAREIAQIF
                     NEKDALARSYLLEGLYHAYNKQWDEALEDYECAKRMADTQTFFGMTARFSAMLFMLSR
                     NYAALEMLLESAPEAMMEYGYQEVVAVYRANTPESVRQAYEQLIKNNPFWNEEVAVAF
                     PEKLAEAAPEIVEQHLEITASKHLKTHDIVSLAMTYEGLALFYDATGNPKKASKFARD
                     AYEVYKQCSYHQACKWIKERFFGTEAVFEDLLAAMAVEIHSEIVHPQRTKDTFERLER
                     SLVKLSNEIAVLRQIVNFSKILTASSDPKEVLDQFNNCIAASIPVNRVGILVVANQMV
                     LYQSTIVLDSTDGDELVQEILTKKCFSAPDPFESRTEFYIDDTKKALLYISNRNLRLD
                     EEEYEKYLAFIDYLEPIMAMAIGNAISYKSSILDPLTRLYTRWYYNQRLEEEMEKALR
                     TNAPLSVIMGDLDDFKHFNDRYGHKTGDEILVTVAHTMRNSCRKYDIIGRYGGEEFIL
                     ILPNTAGEEAYRMAERIRKAIEDETYLPKPLKEMTAELPKTISFGVASTDTVQYDTHT
                     TMVADADKALYHSKNTGKNRTTKFWTMGERVEGTQNIHS"
     gene            4431..5774
                     /locus_tag="PKM49_03985"
     CDS             4431..5774
                     /locus_tag="PKM49_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82006.1"
                     /translation="MRGLKTFILSHRAAKPFLFLLIVLFSSLLMGSEVSFTITGTLPA
                     TLMKAPRSLFHLELITIDPLEKRVVEIYAPIQKPTSLIAEEVSFATELTLRHKKMRAL
                     LFKEETTILISLQKSSLFSIPQILDPRKPGKPFGDEPPLAAAPPARDMTQALDFVLPI
                     EVYPEKRKMLPIERGVKAVEAEQDLAFPLSTQIMNPKGKNTMDHPLSIRIGIDSTWDV
                     LVSLESNVLSFEKGFGQNYTAALLLGETVFQADISAEYVSFSLEKNGFSFRPFLTDEL
                     FGLSILQTEWPFRSVDVGLDFLYPYPLPHLVWYPGIERRFSLSLGVENRRSYGPGFKI
                     YFEPLWLSVFSGISFPDTAFDITVAGGFTIKPISTNLFALVGYNRELYGKIRLETQAF
                     YVKPFHLRARAEAGYERGLYATSELEIQIWSLSLGAGVKWQDDGLVYFLTIAYKF"
     gene            5812..6111
                     /locus_tag="PKM49_03990"
     CDS             5812..6111
                     /locus_tag="PKM49_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HNY82007.1"
                     /translation="MSAQEPIQKEIGPPEKDREKEILEYDIYVKMPVEETHILGYLAE
                     AEEHIINIRHSELIDGAVKVIVPALLLDEALRMLELVKEELPLEIIRYEPNPGYP"
     gene            6086..7303
                     /locus_tag="PKM49_03995"
     CDS             6086..7303
                     /locus_tag="PKM49_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HNY82008.1"
                     /translation="MNRIRDILSLPTPKDRAASIWEIDYDRLYRQGFRFLFFDYDNTL
                     APWKSPLLDDDTKRLFSELIEKGFSINIISNAPTKRVAEVRKGIDERVGVFGNMRKPG
                     VKKLRDVVLRAGAAPSQSVLIGDLFFTDIIVGNRLMMHTFLVNPYMAKLENATKRFTT
                     IFQNILSMIPVFFYKLYFYTIGWFFRLTHLISPHEYARSVFSVDYEQLALHRLDAVVF
                     DLDNTLLPRRIGSLSEEVTALFTRLQRCGFRVFILSNTWRTDRLKNIEKQIGEGQIIR
                     GKMLKPFPYKMRGFLKQAGQDPKKTVIIGDQLFTDILCGNLLHLYTIKVEALDPHDEY
                     WTTKILRFFERRLISFIHFKPKIKETGGWAKAPSPVSKPEMTAGDTDESEEGELLPIG
                     MERMDRRATNAEM"
     gene            7320..8387
                     /locus_tag="PKM49_04000"
     CDS             7320..8387
                     /locus_tag="PKM49_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNY82009.1"
                     /translation="MQSINAAGPGYVPLELFDKYENPLCQRCFRIRHYGSHYHVVSRY
                     YTPQMVLDRLEKCGAVFYVTDLTDLTGTLNPDFLRRLPPRAMVLLNKFDLLPRYLTAS
                     AVKKKVSELYGIEKERLWPISTVNRYGIPGLKDLMLRDAENGFCGYVNAGKSSLINAL
                     LKNPLSEDNEELEKATTSPYPGTTYEPVTYDGGWARLVDVPGMENLSGICRWVDEKDR
                     HLPGKTKRWHRKTCFIRQARAIFFSGLVKVEVEPEETVILQFYFGDNVSVHETNPTRA
                     EEIYAHHCGTLLLPPYSPKFIKTWNWETRAASMEAGYDLCFSGLGWIYCSQGKAKMSV
                     RFPEGLYIEKRTGFIKGTKVR"
     gene            8430..8804
                     /locus_tag="PKM49_04005"
     CDS             8430..8804
                     /locus_tag="PKM49_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNY82010.1"
                     /translation="MKIRPDNTPNVAIAQPSLERQPKVPDSSLVDPAVYQYPRQAEEA
                     KQVQQPLEDIVKGLESSLEKMKGVLRSNLQFEVDKKAEMVIVKIVNKDTGEMVRQIPP
                     ADIVRLIQNLNEFIGALMDERA"
     gene            8832..11390
                     /gene="fliD"
                     /locus_tag="PKM49_04010"
     CDS             8832..11390
                     /gene="fliD"
                     /locus_tag="PKM49_04010"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNY82011.1"
                     /translation="MANFDLTNYINSSVINRTSVGGLASGIDTNSLIDKILDAESVPL
                     KKLTAKKEAMTNRQKALNELSEELTEFRTFTNNWRLESSFLKYQTESTNESIATIQAA
                     PPAKAKNFSFVVDQVARNETYFSAAYVSGNSSTQLQNLGGYHNGVSLEGALTLTVNGK
                     TYDPISYHPSNTLSEVIAQIRADIPEVQVDTVNSAEGVKLFISGADASVDFSITDSGD
                     FLQVMGMTTSYESGFFANGKATNTLANFDGPGTGITANGQLTIRYNGSDIHFDYETTQ
                     TLADLLNEINTDPTLAGKVDVYYATNNTGDALALYIQARNPEDTLSISDAPGAGSKGL
                     LEMLYMTESLFSSTGVDGESGTTTLQSLGVVDGRLTLSVNGVDYFVDYTSSGGTEVLD
                     DVIDAINSTDDLKDSFHAWAEERDGRMYLHLDAKNTDVSISVADNSNLLEVFHIDPSK
                     ATGYNPGALRNGTFHQTAREAQITVDVNGVSTTVTSASNLFENLFDGISVTVKRASSE
                     RVYASVGQDIDGMMDHMKEFIEKYNEVMNNLYLRMNPPETDNTRTDDEKLTEEQKAIK
                     GALKNDPVVRDIFNRLRSLVYSDLDGSNHEVYYSTEVTGKKTDLLSAFGITGGALTVT
                     VTGSSTPYTIEYHGTDTVQSIVDKFRFLNPAIHSKIQEGNGVFSVFVNSTKDFNIVDT
                     PTGESLSFRDFFLNQHSDPVLTYDFLTDIGVGSSDGFTGGYLKIVKGQIMLDETRFRN
                     ALETDAEAVWKTFGASEVFGDTTVKGFATQLSDRLYDITKFGSGYIAQTAGTSGSISN
                     QLRVVNQQIISLSQRLSKRYEALMAKFSFMEKTIQAWQRQSDYITKALEKKSGG"
     gene            11443..12228
                     /locus_tag="PKM49_04015"
     CDS             11443..12228
                     /locus_tag="PKM49_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNY82012.1"
                     /translation="MLTDRAYFSSREKAQAEILLGNIIVNGRKETKPGKQVPSDASIE
                     VLKTDCPYVSRGALKLKGALETFGLSVKGLICCDFGASTGGFTEILLLNGAREVFCVD
                     VGYGQLDWKLRQDPRVVVRERTNVRYLTFESFGHPFDFICGDLSFISLKWILPVASTL
                     LSEGAPSIFLIKPQFELEPQKNVKGIVKGPAYHVEAIERTIRYALDSGLSPEKLSFSP
                     IKGTKGNLEYVILLRKETNVEDKIGNERLREVVDEAERFFLDH"
     gene            12243..14675
                     /locus_tag="PKM49_04020"
     CDS             12243..14675
                     /locus_tag="PKM49_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82013.1"
                     /translation="MKELRPEDLDIDKGYVWKGTSQLESLDFTEESDETKDTFLDGLA
                     LKARNYNIFVTGLSGSGRRSSVKKWLESYAKREKTPDDWIYVNNFEDPWSPVAISLLP
                     GLGKSLKKDWETMIEEALKSLEGVFLSETYRSKLSELNGEFEKQKKRLWSEITQKARE
                     VEYLLQATPTGIAPIPLWDGKPLTNEIHGTLSSDEKRKIEENGVKARHLAEGYLFRLR
                     EYEKDFRTRTEELNRFSAKFAVRSILNEFEQKYQQSEEFLQYKEQVIEDILVQLPNLL
                     AKPELWESLLHRYRINLFVDHSKTVGVPVTFEMNPSFSSLFGKIEFLSKSGYLYTDHT
                     LIKPGAVHLCNGGYLVLDARDVVSNYSLWDTLKKTLYAEKTGVENADTRYGFSSLATL
                     KPQEIPISFKCILIGEPELYDILQELDKDFDKLFRYKVEFDYEIDDSPKNRDQYTRYL
                     ASAVSEKGLLPLEADAVREVARYGMRLASSHKKLSSDYSRITDLLIESDHLSRRENLR
                     SIEGKAVQEAIRRKKARTSLEKRKIEEMIQNDVIRIRTSGKQVGQVNALTVLDLPDHS
                     FGIPTRVTATYALGSGNIIDIHREIDMSGKIFKKAILTLESYIESTFSQKFPLSVKGT
                     LAFEQTYGSIEGDSATMAETVALLSVLAKVPVMQGIAITGSMSQLGEAQAVGGINEKV
                     EGFYSVCKMKGLTGFQGVILPKANLDSLVLEDELLKAIEHRRFHIWCVDTLEEVLEIV
                     MKIQVGKRTRGGNYPKGTLYGKVMDNLFQAFSLYNQGEDGLKTRRKERRKDVAEGQEL
                     IPIETNESADSE"
BASE COUNT         3930 a         3847 c         3940 g         3296 t
ORIGIN      
        1 tcgcgagact tcgtgggctt ccgaagtcag cccgctggtt accccgcgat aaccctacaa
       61 cttgacccac acaacgtttt tccaggacag tactttgtgt tatacttaat ggggtaatgt
      121 cgagtggacg atgattttca taagaaattg aagcaccgcc gcttgagggc aacagcgagg
      181 ctttcacaca taaggacgtg atcagttgcg catcataaag ctcttacgct cgaattatga
      241 tggggacgag tacctcatcg agcacgaagg aacctataaa cgttcgaagc acctgtcgag
      301 ggaggcatac ggcttaagtc tcgataagct tgcggatcgg atcaccgcta tttcaaaagc
      361 gcggagtcgg tttctatacc cggattatgt gggtttggat gtcaatcccc cggtttttca
      421 tttcccgttt cacgaagaat ctgacgtcca taacagcatc cttgaagagg ggcaccgtct
      481 ccaaatcgcg caatcgctgc ttgaaaactt cgaactcttt caacacgcct catcgatcgg
      541 ttttggggtt tgggggcttc cggacctact catttccgat cggttgtact tcttgacgcc
      601 gctttgggtg aactaccggc aaaactcgtt aaaaaacctg ctcaatcggt ctaccgtggt
      661 tgtggcgagt gagctgttgg aggggaaaca gcccacgccg gcctctgacg tctacgttct
      721 cggaaggata ctggaggcga ttttgcccga gcggatactg actgaggttc gtgatcccgt
      781 cacgcaaatg accgatccga acccggcaaa taggccgacc cattactcgc gtatcctttc
      841 gcagccacaa tttgccggtt tgcccaaggc gctttcgacg gcgattaaac gcgaattcat
      901 ctcaccgaat atcatcgagc gcgaaaaaga actggccgcc tttatgtctc tcctgaacga
      961 aagaaaacac cgcggagtca gtctgatcgt gattcgcggc cagactcgcg tcgggaagag
     1021 cagtttcctg acgttggcac aaaacgaaat gtggaacgag ggatggaaga cgattgtggc
     1081 ctccgatttg cagcgattcg cacaggagct cttacagatc gtggagtcgc cggccaaagt
     1141 cggtatcgat gaagaggatt tctcttacct ttgggatttg ggggatgtct acaattttga
     1201 ccgagtgctc tctatcgtgg ggaaactctt gacctcggta gataaacttg ccatattggt
     1261 tgacgatatc gaactggcgg acgaacggta ctctcggctg ttgaaaagca tccgaaatat
     1321 gatcgtggac aatcaaatcg tgatcgcgat gacgcagttg agagagaaag ggactatcga
     1381 ctacgaacac ctcatccacc tacgtccgtt taccgaagag caaacggaaa aactgttgga
     1441 gttgatgttg ggcgaacggt tcgtaaaaaa acataaagac aaggtgcttt ggatttcaaa
     1501 aatcaccggc ggctatcctg ggtacattat cagcctactc aacctcctca atgggtccgg
     1561 aaagctcttt atcgaagacg gacgctgggt tctctccgag gatatgacat cgatcgacgg
     1621 gtttggcgat tacatctcga cgatgttccc ttcgctagaa cccgaagagc agtcgcttct
     1681 gtccaagatc gcgtgtcttt cggaaaaatt cactgccgaa gagcttgacg ctctcggtga
     1741 agcgatcgat ctgcccttgg agaaacttag aaagagcttt tggcactttc aggacctcgg
     1801 tttatttttc acggaggatc acctgtttcg cttctcttta caggagatat gggaacaatc
     1861 ttacgctcgc tgcccgcaag agctgcgata tcgattacac cgccggtttg ccgaatacgg
     1921 gagccagtta aacaagcagg cttggcatta caaggagatc ggtgaaacga ggatggccgc
     1981 gaagatctat ttatgcgcct ctcgtaaagc cttcagaaaa aacgaaggct tcgaaacggc
     2041 tcggggatat atggatgagg tctttcagct gctgaaaccc gaagagatat cgccatcgat
     2101 gctcggctgg tacgcgatcc tttgcgctta tgacggcaag ccttttcccg aatctatgga
     2161 agagtctctg tataataccg aaaactatcg ttacctgtgc atagccaacc gcatcatcaa
     2221 aggagagtac cagaaagcgg atgagatgtg cgggcggcaa gcgggtttgt ttaaggataa
     2281 aaaactatca caagcgctct gggttacaca gatgatctac gcctgtgcta cgttgaaagt
     2341 gaaaatcgga gagttagaca acgcgctgtc gctcctcaac ttggttctgg agcgtgtctg
     2401 caataaaaaa accgacgcgc ataacagatt ggaaatcatg gcttacaact taattgcgca
     2461 ggcttacgag cgaaagaaca attggcagcc tgcgtattcc atatgtgaag ccaacatcga
     2521 gcgggccaag aagtatcata tgtcttacct gttacctgat gtcttcctga ccgccggcaa
     2581 catcgtcttt cctttggggc ctggttatgc ccaaccgcac tacgaaaacg cgataaaaat
     2641 cgccaaagta ttcttttctg cccaaaaatc cctccaacct acgctagagc tggctaacgg
     2701 ctttctgtac agcggtgaaa ccgcgaaaat gtttgactac cttgaaaaag cccgggagat
     2761 cgcccagatc ttcaatgaaa aagacgccct agcgaggagt tacctcctcg aggggctgta
     2821 tcacgcctac aacaagcaat gggacgaggc gctagaagat tacgagtgtg ccaaacgtat
     2881 ggccgatacc cagacttttt ttgggatgac cgctcggttt tctgcgatgc tctttatgct
     2941 cagtcggaat tacgcggctt tggaaatgtt gctcgaatcc gctccggaag cgatgatgga
     3001 gtacgggtac caagaagtag tggcggttta ccgcgcgaac actccggaaa gtgtacgaca
     3061 ggcttacgag cagctcatca agaataaccc gttttggaat gaggaggttg ccgtcgcttt
     3121 ccccgaaaag ctggcggagg cggcacccga aattgtggag caacatctcg aaattaccgc
     3181 atccaaacac ctgaaaacgc acgatatcgt ctctttggcg atgacctacg aaggcttggc
     3241 ccttttttac gatgcgacag gaaacccgaa gaaggccagc aaattcgctc gggatgccta
     3301 tgaggtctac aagcaatgct cttatcacca agcctgcaag tggataaaag aacgattctt
     3361 cggtaccgaa gcagtttttg aggacctttt ggcagcgatg gcggtcgaga tccattccga
     3421 aatagtgcat cctcaacgga cgaaagacac cttcgaaaga ctggaacgaa gcctggtgaa
     3481 gctcagcaac gaaatcgccg tgctacgcca gatcgtcaac ttttcgaaga tactgaccgc
     3541 ttcttccgat cctaaagaag ttttagacca gttcaacaac tgtatcgcgg ccagcattcc
     3601 ggttaatcga gtcgggattc tcgtcgtcgc caatcaaatg gttctctatc agagcacgat
     3661 cgtattggac tcgaccgacg gtgatgaact ggttcaagag attttgacga agaaatgctt
     3721 cagcgcgccg gaccccttcg agtcgcgcac ggaattctac atagacgaca cgaaaaaggc
     3781 gctcctgtat atctcgaacc gaaacctgag actcgatgag gaagagtacg aaaagtactt
     3841 ggccttcatt gactatctgg aaccgattat ggctatggcg atcggaaacg cgatttcata
     3901 caagtcttcg attctcgacc ccttgacgcg cctgtacaca cggtggtact acaaccaacg
     3961 gttggaagag gaaatggaga aagcgttgag gacaaacgct cccctttccg tcattatggg
     4021 agatttggac gatttcaagc acttcaacga ccgatacggc cacaagaccg gagacgaaat
     4081 attggtgacg gtcgcgcata cgatgcgaaa cagttgccgg aaatacgaca tcatcggccg
     4141 atacggagga gaagagttca ttttaatcct tccgaatacg gcgggcgaag aggcatatcg
     4201 gatggcggaa cgcatccgca aagccataga ggatgaaacc tatctgccga agccgctaaa
     4261 ggaaatgacc gcagagttgc cgaagaccat cagttttggc gtcgcctcta ccgataccgt
     4321 tcaatacgat acgcacacca cgatggtcgc cgatgccgac aaagcgctat atcattcgaa
     4381 aaatacgggc aagaatagaa cgaccaagtt ttggacgatg ggagagcgcg ttgaggggac
     4441 tcaaaacatt cattcttagt caccgtgcgg cgaaaccgtt tctatttttg ttgatcgtcc
     4501 tcttttcatc gcttcttatg ggctccgagg tgagcttcac catcacgggc accttgcccg
     4561 ctaccctaat gaaagcgccc cgttcgttgt ttcatctgga actgatcacc atcgatcctt
     4621 tggagaaacg cgtggtagaa atctacgcgc ccattcagaa gccgacctct ttgatcgccg
     4681 aagaggtatc tttcgcgacc gagctgacac taaggcataa aaaaatgcgg gcgctcctct
     4741 tcaaagaaga gacgacgatt cttatctccc tccaaaagag ctccctgttt tcgattccac
     4801 aaatcctaga ccctcgaaag cccggtaaac cctttggcga cgaaccgccg ctcgccgccg
     4861 ctccgcccgc gcgcgatatg acccaggcgc tcgacttcgt cttgccgatc gaagtctacc
     4921 cggagaaaag aaagatgctg ccgatagaga gaggggtaaa agccgtcgaa gcggagcaag
     4981 atctcgcctt ccccttgtcc acgcagatta tgaaccccaa ggggaagaac acgatggacc
     5041 atccgctatc catccgcatc ggaatagaca gcacgtggga cgttctcgta tcgttggagt
     5101 cgaacgtcct ttcctttgag aaaggatttg gtcaaaacta tacggccgct ctcttactgg
     5161 gcgaaacggt gtttcaggcc gacatttcgg ccgaatacgt ctccttttca ttagaaaaga
     5221 acgggttttc cttccgccct ttcctcaccg acgaactgtt cggcctttcg atcctacaga
     5281 cagaatggcc gtttagaagc gtcgatgtcg ggctcgattt tctctacccc taccccttgc
     5341 cgcatttggt atggtacccc ggaatcgagc ggcgcttttc tctctccttg ggcgtcgaaa
     5401 accgacggtc atacggcccg ggcttcaaaa tctacttcga gcccttgtgg ctctccgtct
     5461 tcagcggcat atcattcccc gataccgcct tcgacatcac cgtggcgggc gggtttacaa
     5521 tcaaaccgat aagtacgaac ctctttgccc tcgtgggata caatcgagag ctgtacggca
     5581 agattcggtt agaaacccaa gcgttttatg taaaaccctt tcacctgcgc gcaagagcag
     5641 aagccggata cgaaaggggc ctttatgcga cttcggaatt agagatacag atttggtctc
     5701 tgtcgctggg agccggtgtg aaatggcaag acgatggatt agtctacttc ctgaccatcg
     5761 cgtacaagtt ttgatcttcc gtctatatat acgattgagg tgtgtgagat attgagtgcc
     5821 caagaaccta ttcaaaaaga gatcggaccg ccggaaaaag accgggaaaa agagattctg
     5881 gagtatgaca tctacgtgaa gatgccagtg gaagagacgc atattctagg ctatctggcg
     5941 gaagcggaag aacacataat caatattcgc cactccgaac tcattgacgg ggccgtgaag
     6001 gtgatcgttc cagccctgtt gctggacgag gccctcagaa tgttagaact ggtcaaagaa
     6061 gaactccctt tggaaatcat acggtatgaa ccgaatccgg gatatcctta gcttaccgac
     6121 acccaaagat cgggcagcct ctatctggga aatcgactac gatcgtttgt accgacaagg
     6181 ctttcgtttt ttgttcttcg actacgacaa tacgctcgcc ccttggaaaa gcccgttgct
     6241 cgacgacgac acgaaaagat tgttttccga gctgatcgag aaggggtttt ctatcaacat
     6301 catcagcaac gcgccgacta aacgcgtggc cgaggtccgc aagggaatcg acgaacgggt
     6361 gggcgtgttc gggaatatgc ggaaaccggg agtaaaaaag ctcagagatg ttgtgctgcg
     6421 cgcgggggcc gcgccctctc aatcggttct gatcggcgac ctgtttttta ccgatatcat
     6481 cgtgggaaat cggttgatga tgcacacctt cttggtgaac ccctatatgg cgaagctgga
     6541 gaacgcgact aaacggttca cgactatttt ccaaaacatc ctcagcatga ttccggtttt
     6601 tttctataag ctctatttct atacgattgg ctggtttttt cgtttgactc acctgattag
     6661 cccgcacgaa tatgcccggt cggtcttttc tgtcgactat gaacaattgg ccctacatcg
     6721 gttggacgcg gtcgttttcg atctggataa taccctcctt cctaggcgaa tcgggtcttt
     6781 aagcgaggag gtgactgcgc tcttcacccg tctgcagcgg tgtgggtttc gcgtcttcat
     6841 cctatccaat acgtggagga ccgatagact caaaaacatc gaaaaacaga tcggcgaagg
     6901 gcagatcatc cgcgggaaga tgctgaaacc ctttccatac aagatgaggg ggttcttaaa
     6961 acaggcggga caagacccaa agaagacggt tatcatcggc gaccagctct tcaccgatat
     7021 cctatgcggg aacctactac acctctacac gatcaaagtg gaagccctag acccacacga
     7081 cgagtattgg acgacgaaga tcttacggtt tttcgaaaga cgactgatct ccttcatcca
     7141 tttcaagcct aaaataaaag aaaccggcgg gtgggccaaa gcgcctagcc ccgtttccaa
     7201 acccgagatg accgccggtg ataccgacga atccgaggaa ggagagctcc ttcctatcgg
     7261 aatggaaagg atggatcgac gtgccacaaa cgccgaaatg tgaggggtgc ggtgtttctt
     7321 tgcagtcaat aaacgccgcg ggaccaggct atgtcccgtt ggaactgttc gataaatacg
     7381 agaaccccct ctgtcagcga tgctttcgga tacggcacta tggctctcac tatcacgtgg
     7441 tgagtcgtta ttacaccccc caaatggttc tcgataggct cgaaaaatgt ggcgccgtct
     7501 tttacgtgac agacctgacc gatctgacgg ggacgctgaa tcccgatttt ctccgccgtt
     7561 taccgcctcg ggcgatggtg ttattgaaca aattcgacct cctcccgcgc tatctcaccg
     7621 ccagcgcggt gaaaaagaag gtttcggagt tatatggtat agagaaagaa cggttatggc
     7681 cgatcagcac ggtgaaccgt tatgggatac cgggtttgaa ggatctgatg cttcgtgatg
     7741 cggagaacgg cttttgcgga tacgtcaatg ccggtaagtc ctcgctgatc aacgcgctgt
     7801 tgaaaaaccc cctcagcgaa gataacgaag aactcgaaaa ggcgaccacc tctccatacc
     7861 ccggaactac ctacgaaccg gtgacctatg atggaggatg ggcgcgactt gtggacgttc
     7921 caggcatgga aaacctgtcc ggcatctgtc gatgggtcga cgaaaaagac cgccacttgc
     7981 cgggaaaaac caagcgttgg catcgaaaaa cttgctttat ccggcaggca cgcgcgatct
     8041 ttttcagcgg cttggtcaag gtggaggtcg aacccgaaga gaccgtcatc ctccagttct
     8101 atttcggaga taacgtcagc gtccacgaaa cgaatccgac gcgggccgaa gagatctacg
     8161 cgcaccattg cggcaccctg ctgctacctc cctattcccc gaaattcata aaaacctgga
     8221 attgggaaac gcgagcggcc tctatggaag ccggatacga tctttgtttc agcggtttag
     8281 gatggatata ctgctcacaa ggtaaagcaa aaatgtctgt acgttttccg gaaggtttat
     8341 atatcgagaa gagaacagga ttcataaaag ggactaaagt ccgttaggac tattgccgat
     8401 aaaaggtatc ataccatgga ggggagatta tgaaaatccg accggacaac acgccaaacg
     8461 tggcgatcgc ccagccatct ctggaacgac agcccaaggt accggactcc tcactcgtcg
     8521 acccggccgt gtatcaatat ccacgccaag cagaggaggc caagcaggtt cagcagccgc
     8581 tcgaagatat cgtcaaaggt ttagagagca gcctggaaaa gatgaaaggg gttttgagaa
     8641 gcaacctaca gttcgaagtg gacaagaaag ccgaaatggt cattgtgaag atcgtgaaca
     8701 aagacaccgg cgagatggta cggcagattc ctccggccga catcgtccga ctgatccaaa
     8761 acctcaacga attcatcggc gccttgatgg acgaacgagc gtaaagtccc ttaaaaaagg
     8821 gggttctgaa tatggcgaat tttgatctga cgaattatat caattcgtcg gtgattaatc
     8881 gcacttccgt cggcggtctc gcaagcggaa tagacaccaa ttctctgata gacaaaatac
     8941 tcgacgccga aagcgtgccg ttgaaaaaac tcacggcgaa gaaagaggcg atgaccaacc
     9001 gccaaaaagc cctgaacgag ctgtccgagg agttaaccga gttcaggact tttacgaaca
     9061 actggcgttt ggaatcgagt ttcctgaagt accagacaga atcgaccaac gagtcgatcg
     9121 cgacgattca ggcagcccct ccggcgaaag cgaagaattt ttctttcgtc gtcgaccagg
     9181 tcgcccgaaa tgaaacctat ttttccgccg cctacgtatc ggggaatagc tcgacgcagt
     9241 tgcagaatct cggcggatac cacaatgggg tctcgctcga aggcgcgtta acgttgacgg
     9301 tcaacgggaa gacctatgac ccgatctcct accacccttc gaacacccta tccgaggtga
     9361 tcgcgcaaat cagagccgat atccccgagg tgcaggtaga tacggtgaac tccgccgagg
     9421 gggtgaaact gttcatctcc ggcgccgacg cttcggtgga cttttcgata accgattccg
     9481 gcgacttcct tcaagtgatg gggatgacga cgtcctatga atcaggcttt ttcgcgaacg
     9541 gaaaagcgac gaatacgctc gccaattttg acggccctgg aaccggtata acggccaacg
     9601 ggcagctgac cattcgctac aatgggtcag atattcattt cgattatgag accacgcaaa
     9661 ccttggcaga cctgctgaac gaaatcaaca cagaccccac tttagccgga aaagtggacg
     9721 tttactatgc gacgaacaac accggagacg cgttggccct gtacatccaa gcgagaaatc
     9781 ccgaggatac cctttctatc tccgatgccc cgggagcggg ttccaaaggg ttgctcgaaa
     9841 tgttgtacat gaccgaatcc ctattctctt ctaccggcgt cgacggggag agcggcacga
     9901 cgacgctcca gtcgttgggg gttgtggacg ggcggctgac gctgagcgtg aacggcgtcg
     9961 actactttgt cgattacacc tcatctggcg gtaccgaggt gttggatgac gtaatcgacg
    10021 ccatcaactc gaccgacgat ctcaaagact cctttcacgc ttgggcggaa gaacgtgatg
    10081 gtaggatgta cttgcacctc gacgcgaaaa ataccgatgt ctccatttct gttgccgata
    10141 acagtaacct attagaagtt ttccatatcg atcccagcaa ggcgaccggt tataaccccg
    10201 gcgccctacg gaatgggaca ttccaccaaa cggctcggga agcgcagatt acggtcgacg
    10261 tcaacggggt ttccacgacg gtcacgtcgg cgtcgaacct cttcgaaaac ttgtttgacg
    10321 gcatatccgt gaccgtcaag cgcgcgagct ccgaaagagt gtacgcttcc gtcggccagg
    10381 acatagacgg aatgatggac catatgaagg agttcatcga aaagtacaac gaagtgatga
    10441 ataacctcta cctccggatg aatcctccgg aaacggacaa cacgaggacc gacgatgaga
    10501 aattgaccga ggaacagaaa gccatcaaag gggctctgaa aaacgacccg gtcgtccgag
    10561 acatcttcaa ccgtctgcgt tctctcgtct attcggacct ggacggctcg aatcacgagg
    10621 tctattactc cacggaggtg accggtaaaa aaaccgatct cctgagcgct ttcggcatca
    10681 cggggggcgc cttaacggtg acggtcaccg gctcttcaac gccttatacg atcgaatacc
    10741 acggcacgga caccgttcaa tcgatcgtcg acaaattcag attcctcaac cccgcgatcc
    10801 attcgaagat acaagaggga aatggcgttt tcagcgtctt cgtgaatagc acaaaagact
    10861 tcaatatcgt cgacacgccg accggcgaat ccttgtcctt ccgtgatttc ttcttgaacc
    10921 aacactccga tccggtgctg acctatgatt ttctgaccga tatcggtgtc ggttcctcgg
    10981 atgggttcac cggcggatat ttaaaaatcg tcaaaggcca gattatgctg gatgaaaccc
    11041 gttttcgaaa cgctttggaa accgatgcgg aagcggtatg gaagaccttt ggcgcgagcg
    11101 aggtcttcgg agacaccacg gtgaagggct tcgcgactca gctctccgat agactctacg
    11161 atatcaccaa gttcgggagc ggatacatcg cccaaaccgc gggcacttcc ggatccatca
    11221 gcaaccagct gcgggtggtg aaccagcaga tcatctcgct gagccaacga ctatccaaac
    11281 gttacgaggc gcttatggcc aagttttcct ttatggaaaa gacgatccaa gcgtggcaaa
    11341 ggcagagcga ctatatcacc aaagccttag aaaagaaatc aggcggataa tcgtataaca
    11401 ccgaaaactc ccaagcgcgt ttaccaaacg aggctcgatc tattgctcac ggatcgagcc
    11461 tatttttcgt cacgagagaa agctcaagca gaaatcctat tgggtaacat catcgtgaac
    11521 gggcgtaaag aaacgaagcc cggaaagcag gtgccttccg acgcttcgat cgaggttttg
    11581 aagaccgact gtccgtacgt cagtcgcggc gcgctgaagt tgaaaggggc gctggagacc
    11641 ttcggccttt ccgtcaaggg actcatctgt tgcgacttcg gcgcttccac cggtggattc
    11701 accgaaatcc tcctattgaa cggcgccaga gaagttttct gcgtggacgt gggttacgga
    11761 caattggatt ggaagctgcg gcaagacccg agggtggtgg tccgagaacg aaccaacgtc
    11821 cgctatttga ccttcgagtc cttcgggcac cccttcgatt tcatctgcgg cgacctctcc
    11881 ttcatttcct tgaaatggat cttgcctgtg gcctctactt tgctctctga aggcgccccg
    11941 tcgatatttc tcatcaaacc gcagtttgaa ttggagccgc aaaaaaacgt caaaggtatc
    12001 gtcaagggcc ccgcatacca cgtcgaagcg atcgagcgga cgatccgcta cgctttggac
    12061 agcggcctat cccccgaaaa actctccttt tcgccgatca aggggacgaa gggcaacctc
    12121 gaatatgtta tactcctgcg taaagaaaca aacgtggaag acaagattgg aaacgaacgt
    12181 ctcagagagg ttgttgacga agcggaacga ttcttcctgg atcattgaga ggtggaaaaa
    12241 gcttgaaaga attacggcct gaggatttgg acatagacaa agggtatgtg tggaaaggga
    12301 cctcgcagct tgaatctctg gacttcaccg aggaaagcga cgaaacgaaa gatacctttc
    12361 tcgacggcct agctttgaaa gccagaaact acaacatctt cgtaaccgga ctcagcggct
    12421 ccggacggcg ctcctctgtg aagaaatggc ttgaatccta cgcgaaaaga gagaaaacgc
    12481 cggacgattg gatttatgtg aacaatttcg aagacccttg gtctcccgtg gcaatctccc
    12541 ttctacccgg tttgggcaag agcctgaaaa aggactggga gacgatgatc gaagaagcgc
    12601 tgaaatcgct cgaaggggtg ttcctctcgg agacctatcg gagcaagctc tcggagctca
    12661 acggcgagtt tgaaaagcaa aaaaagcggc tctggtcgga gatcacccaa aaagcgagag
    12721 aggtggagta cctgctgcaa gcaaccccga ccggtatcgc tccaattccg ttgtgggacg
    12781 gaaaaccgtt gacgaacgaa atacacggca ccctcagctc agacgaaaag cggaaaatcg
    12841 aggagaacgg cgtcaaggca aggcatttgg cggaagggta cctgtttcgg ttacgagagt
    12901 atgaaaaaga cttccggacc cgtaccgaag aactgaaccg cttcagcgcc aagttcgccg
    12961 ttcgttcgat tctgaacgaa tttgagcaga agtatcagca gtccgaagaa ttcctccagt
    13021 acaaagagca ggtgatagag gacatcttgg tgcagctccc caatttactg gccaaacctg
    13081 agctatggga aagcctgttg catcggtatc ggatcaacct cttcgtggat cacagcaaaa
    13141 cggtcggcgt ccccgtgaca ttcgagatga acccgagttt ctcatctctc ttcgggaaaa
    13201 tcgagttcct gtcgaaaagc ggatacctgt atacggacca caccctgatc aaaccgggcg
    13261 ccgttcacct gtgcaacggc gggtacctcg ttttagacgc ccgagacgtg gtttcgaact
    13321 actccctttg ggacaccttg aagaaaacgc tctatgccga aaagaccggt gtggagaacg
    13381 cggacacgcg ttatgggttc tcctcgctgg ccacgctaaa acctcaagaa ataccgattt
    13441 ccttcaagtg catcctcata ggcgaaccgg agctgtacga tattctgcaa gaactggaca
    13501 aagacttcga caagctgttt cggtataaag tggagttcga ctatgagata gacgactccc
    13561 cgaaaaacag agaccaatac acgcgttact tggcttctgc cgtctctgag aaaggtctgt
    13621 tgccattgga ggcggacgcc gttcgagagg tggcgcgata cggtatgagg cttgcaagct
    13681 ctcacaagaa gctttcgagc gactactcgc gtatcacgga cctactcatc gaaagcgacc
    13741 acctgagccg acgtgaaaac ctccggtcga tcgagggaaa ggccgtacag gaggctattc
    13801 ggaggaagaa ggcccgaacc agcctagaga agagaaaaat cgaagagatg atccaaaacg
    13861 acgtcatccg tatacgcacg agcggaaagc aggtggggca ggtcaacgcc ctgactgtcc
    13921 tggaccttcc cgatcactcc ttcggtatcc cgacccgagt cacagcgacc tacgccctag
    13981 gcagcggcaa catcattgac atccaccgcg agatcgatat gagcggtaag atattcaaga
    14041 aagcgatttt gacgttggaa agctacatcg agtccacctt ctcccaaaaa ttcccgctct
    14101 ccgtgaaagg caccctcgct ttcgagcaaa cctacggctc gatcgaaggc gacagcgcga
    14161 cgatggcgga aacggtggcg ctgttgtccg ttttggcgaa ggttccggtg atgcagggca
    14221 tcgcgattac gggttcgatg agccaactgg gggaagccca ggcggtgggc ggaatcaacg
    14281 aaaaggtgga gggtttttac agcgtctgca aaatgaaagg gctgaccggc tttcaagggg
    14341 tcatcttgcc taaggccaac ttggactcgt tggtactgga agacgaacta ttgaaggcga
    14401 tagaacaccg acgctttcac atttggtgtg tggacaccct tgaagaggtg ttggagatcg
    14461 tgatgaagat ccaggtcggg aaacggaccc gtgggggcaa ctatccgaaa ggcacgctgt
    14521 atgggaaagt gatggacaac ctcttccagg ccttcagtct atacaaccag ggggaagatg
    14581 ggctgaaaac gagaaggaaa gaacggcgaa aagacgtggc ggaaggccag gagttgattc
    14641 cgatagagac aaatgaaagc gccgattcgg aatagcgtgg gtcaagatat tgggtgacat
    14701 aactaagaag gttatcgcgg ggtaaccaac gggaccccgc gatgctcgtg cggggtaatc
    14761 gcggggtaac cgaagggacc ccgcgatccc ctttttgttc tctttacacc gcgatgatgt
    14821 cgtggtatcg cgccgtcgcg aaaagaaggg gcgcttacgc cgcgatgatc gtacggagtt
    14881 atcgcggggt aagtggtttt tacgaccttg cgattcgccc tttgcttttt ttgccccgcg
    14941 accagctcga atagaagcgt atcgcgccgt cgcgaaaaga aggggcgctt acgccgcgat
    15001 gatcgtacgg agt
//
LOCUS       DAOPXN010000047        14938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4083_length_14938_cov_5.219243, whole genome shotgun sequence.
ACCESSION   DAOPXN010000047 DAOPXN010000000
VERSION     DAOPXN010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 14938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14938
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_14938_cov_5.219243"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="PKM49_04025"
     CDS             complement(<1..1070)
                     /locus_tag="PKM49_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82014.1"
                     /translation="MEKITFSYRKTFLLGFGFFSISLLWSIYNSYIPIFLKEFSLSSL
                     LVGFIMTFDNILAIVLQPYIGFLSDQTRTRFGRRMPFIMVGAPIAAVFFLLIPFAKSL
                     NIGWMITTILIMNLAMAIFRAPVIALMPDTVPSQSRSKANGVVNFMGGLGALLAFLVG
                     SKLYEINKSLPFIIGGVALVVSCLLVFVFVKEPIAETDDSSEKPSKKTAKIGFIEAFK
                     ELWTALKELFLSKEKSELWLLLAIFLWFCGFNAIETFFTSYGKYHLGIEESKASFALG
                     LFSLTFMLFSIPAGFIGTKYGRKKTILAGIVILLIMNGIIGLLTDFSLIQATLIFGGF
                     GWACININSLPMVVDMVEQEKVG"
     gene            complement(1114..2817)
                     /locus_tag="PKM49_04030"
     CDS             complement(1114..2817)
                     /locus_tag="PKM49_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY82015.1"
                     /translation="MKTFLVYLSIKPEMAKRSNLPLRLPLLLEDFVELGPASEVRPEM
                     VLRGLRAQIKAGTAKGATSEEGTLDYYASYFLFFLYENVKSALSEKAFDKAGALLEEA
                     RAFLDRFPHLVKAHDARLDFYQALYAFRTGFPGQAETVLRKIVTENPKNPYFAMELAR
                     LLEEQDDVDSALEVYSKVVEEDPAFLPALFSMGEIFLRTGDYDRAEDSYKACIQSGAD
                     FVPPYARLGVIYNNRQQYTKAVHILTQGLSRAPDDVPMHYNLSFALQKISKPFTALAH
                     LKAALVQNPDSIPVLNEIGVLYRKLGLFEESLDALKKARSADEENLGILWNLLWTTML
                     ISKEEFDHLLIRSLPFLTRHGFDVERFSPFHNRRTSRPFDLFTFLAELAGAYEGIPGY
                     LEERLHGLLVGRMPTEEMDSSEDLIVLDWIVDLFENYDADPIELFRGILLVNVALTRS
                     TRWMAFCFSLFELVTHRAGFPFDLDKTVEALALTAQEYDWDLASRISSIDQECFSDWT
                     DLVDAPPTLSNLYELIRWVLNLLWIDPASEELETLPVEPSFKRLIGYVFHKTQEAPAG
                     T"
     gene            complement(2814..3074)
                     /locus_tag="PKM49_04035"
     CDS             complement(2814..3074)
                     /locus_tag="PKM49_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNY82016.1"
                     /translation="MKSDNDLLISLGADAFVTKDRIQAVFSPENAAMKKIKMVADQER
                     KVINLTYGNETKSILLMDSGHILLTMIEVDQLLSIYEKGKTD"
     gene            complement(3071..4069)
                     /gene="galT"
                     /locus_tag="PKM49_04040"
     CDS             complement(3071..4069)
                     /gene="galT"
                     /locus_tag="PKM49_04040"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HNY82017.1"
                     /translation="MPELRKDPITNRWVIIATERAKRPMDFGKAPEEAKGAFCPFDYG
                     NEYTTPPEILAFRPKDSQPNLPGWWIRVVPNKFPALEPNLKMQRYGHGMYDAMTGFGY
                     HEIVIETPDHNATIATCEGKQIEEMIWAYKARYKALAKDPGIKYILIFKNHKRDAGAS
                     LAHSHSQIIATPIVPKRVQEELTGAQTYFQYKERCVFCDIIYQERMESVRIVEETDQF
                     ISINPYASRFPFETWILPKNHSHDFGEIGEVQVQEFATVLKKSLLRIYKSLDNPPYNF
                     LLHTAPTTGEAKQSFHWHLEIVPRLTKVAGFEWGSGFYINPTPPEDAAKYLKGVEI"
     gene            complement(4088..5569)
                     /locus_tag="PKM49_04045"
     CDS             complement(4088..5569)
                     /locus_tag="PKM49_04045"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNY82018.1"
                     /translation="MRIAMMSYEVYPFVKVGGLADVLGSLPKYLERLSSRSGSQPMEI
                     DIYMPYHRAVSKNAKPFGYSIEKIGSPFPVPFMQTIQEVQLYRSRLPDSDVNVYFIGN
                     DHFFSGEKVYGYEDQGEQAIFFNVGALEAIRVLGKSYDVIHAHDWQSGLIPVYIKTLL
                     REEPMFQKALTVFTIHNLGYQGKFPSMYLNMAGLPHYLFNVDALEFYGEINFLKGGVL
                     FSDIVTTVSPRYAQEIQTKAFGEKLEGVLSIRSDALYGILNGIDYDIFDPTKTNLVPY
                     AFTPNDLSGKARNKRLLQERLGLEVNPDVPVFGLISRLVAQKGLDILSKITPFFLMQD
                     VQLVVLGTGDAEYEELVQEMAAENPKKVSANLKFDIDLANLIYAGSDIFLMPSRYEPC
                     GLGQMFSLRFGTIPVVHYVGGLADSIIEFDEKEKTGNGFGFSEYRESELLLSIFRALY
                     FYRRKESWETLIKNAMTDDFSWERSAKEYVYIYTEGLKNKRAL"
     gene            complement(5584..6207)
                     /locus_tag="PKM49_04050"
     CDS             complement(5584..6207)
                     /locus_tag="PKM49_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNY82019.1"
                     /translation="MFVREWYNREKPRLVGDMHPKDVKKFFSNEDVNSLPVVDERGFY
                     LGILHYEDFKDAEKTEKAIDYSSNPEFFVEEDDTLEDVALLLMENHEMLVPVLDSQRR
                     LLGCITIFEILEAFTQVAAFDEGGMSISLLLEDTPGKLKTLVDLLAFYNINILSILTY
                     KKEHLSTGGKAFREVYLRIDSNNVSEVKEILEKIRVPINYLKKREAY"
     gene            complement(6292..7689)
                     /gene="gltA"
                     /locus_tag="PKM49_04055"
     CDS             complement(6292..7689)
                     /gene="gltA"
                     /locus_tag="PKM49_04055"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNY82020.1"
                     /translation="MLNRSKQKTKLIERAPEERRKDFLEVNDGYSLTEAVSEADRCLQ
                     CRNAPCIQGCPVAIDIPGFIKAIQEKDLERSWEILYATNRLPAICGRVCPQEKQCEER
                     CTVGKIKNSEPVAIGRLERFTADWKRKAGFSPAPVELTHTERIAVVGSGPAGLTVAAE
                     LAQNGYAVEVFEALHEPGGVLTYGIPEFRLPKEIVRAEIEGIRKLDVVLRTDFIVGKT
                     IFFEEIRKRYQAIFLGIGAGAPKFMNVPGTNLNGVFSASEFLTRVNLMKAFRFPHADT
                     PITIGKNVIVVGGGNVAMDAARSALRLGAQTVKIVYRRTETELPARWEEYHHAMEEGV
                     EFLWLSNPIAYLPDSDSRVKGARIQKMGLGEPDASGRRKPVPLPGVEETLECDCVIEA
                     IGQSAHRILSEGFKEVRLNRWGYIEVDPETLMTSVPGVFAGGDIVTGAATVIEAMGAG
                     KTAAKGIMNYLRTIG"
     gene            complement(7706..8593)
                     /locus_tag="PKM49_04060"
     CDS             complement(7706..8593)
                     /locus_tag="PKM49_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNY82021.1"
                     /translation="MTSPIKPPQEIVSTITKIAPKIYDMTVVSTSEEGSAALISRNAK
                     AGQFVMIRADEEGERIPLTIAQTEAKKRAVRLIFQVVGTSTHKLSLLKPGDQVLNLAG
                     PLGKPSEVVEGKKVLVIGGGVGIAAILPIAGSLYHAGNDVTVVLGARTRDLLILKDEF
                     KPIAHRLVLCTDDGSEGRKGFVTDAMNDLVANTGAYDVGWAVGPTIMMKMCSEIAKRL
                     DFPLWTSLNPIMVDGTGMCGGCRVLVGNEMFFACVDGPEFDGRLVDWDNLLLRQKQFK
                     TEEADSFHHCRLENQLHSR"
     gene            complement(8605..11388)
                     /gene="ileS"
                     /locus_tag="PKM49_04065"
     CDS             complement(8605..11388)
                     /gene="ileS"
                     /locus_tag="PKM49_04065"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNY82022.1"
                     /translation="MEYKDTLQLPKTSFPMRANLPQKEPEILRYWQEIGLEAYISEER
                     ANSPKKVLHDGPPYANGEIHVGTALNKILKDCIVKYWTMQGYQSPFKPGWDTHGLPIE
                     HRVFTELGDKSKKMDKMEIRKACQAYAKKYVDLQREEFIRLGIRGKWADPYLTFLPAY
                     EAGVYEVFRELVKKGNVYRSKKVVHWCYSCETALAEAEIEYAEDTSDSIYVKFALEGA
                     ENTYIVIWTTTPWTLPANLAIAVHPDFDYAYVDIDGETWVLAEKLVEPLMKKTNRSRY
                     RVLRTVKGTQLENKRARHPFIDRDSLIVLADYVDLETGSGCVHTAPGHGQEDYLTGLK
                     YGLDIYSPVNSQGKFTEDVKDYEGQFIFSANPAIVQHLKESGHLIWSEKYKHPYPHCW
                     RCKKPVLFRATEQWFIRVDGDDLREKALDQIDHVHWVPDWGKNRILAMVRDRPDWCIS
                     RQRVWGIPIPAFFCQSCGNTVMTEETISHLIPIVEREGTDVWFEQPPESLLPEGFRCP
                     YCGGKNLKKEEDILDVWIDSGSSFESVMAADPELGFPAELYLEGSDQHRGWFQSSLFL
                     SVAKRGIAPYKEVVTHGFIRDGKGIKMSKSLGNVIVPHEVYDKYGAEILRLWTATADY
                     RNDINLSMDIILQQVDVYKKIRNTMRFLLGNLSDFVPERETVPYDRLSSFDRWALSRL
                     NDLVETVTESFDTFEFYKGIQTINKFVISDLSAVYLDILKDRLYVEGKDSPVRKSGQT
                     VLLEILKTLTLLLAPILTFTSEEVYQFFPAALKQKKTIQAESWPIRHPEWKGNHIGSN
                     GLTLNQKWERLLELRERVNASLESVRTAGVIGHSLDASVSISVKDPELYRLLQEEHAF
                     LSDFFIVSECKTTFVENADSPLEIGVQRAQGAKCQRCWKYDLKIGEDQIHPDICPRCA
                     QIIRQQTYANRLT"
     gene            complement(11442..11810)
                     /locus_tag="PKM49_04070"
     CDS             complement(11442..11810)
                     /locus_tag="PKM49_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY82023.1"
                     /translation="MEKPRIMVVEDEDNMRFLISEELSDEGYLVDTAANGSECLRKFE
                     EGSYDLVTIDIEMPDMSGLELAGLLRERKIPVRIILLTAYSHYKSDLSSWAADAYIVK
                     SSNFLELKSTIKELLNATNA"
     gene            complement(11851..14628)
                     /locus_tag="PKM49_04075"
     CDS             complement(11851..14628)
                     /locus_tag="PKM49_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNY82024.1"
                     /translation="MIKIIHTLLNLDIVHSDEGVNLLAEEIGALLNVQECSIFVYEKE
                     KLAFRLIGTSILDRRFVKQFKIKEFQVPSIQDLKRGSSVFLEKKALEDENRKFESENL
                     LICPILDDGLLDSLIFLGDESPEKLQALDIKNYDFFRILGKIIRLSIENRLLYENVGK
                     LLGLTRLSDVVTKTEAISELYERSLKKTCDVLNVENGSIWLANAEGDLEIGYWYGLNE
                     NQLLKRTIPVGHGMVGWCYKHKKPMLSISVKKDPRAALDMLAVDIKSSIAVPLFHENE
                     IFGVIILINRKDQELYRPYKHFDEFDLALLEDFAARISLIVSKMTYYNQLSKDYKELS
                     ELSASNESLLETQKEQVNLLKNITAINRAMRESYDIQNIYTILLLGATEYAGLGFDRA
                     MLLAKDIKSQTLIPRYWMARREQEGTRGSGSATGKYGNLSQHLREKAIMTDLKEYESD
                     KLRNRVFPYKSHAIFEKAVLRKKIVQVTPEMIREWGNEYRELKNLLETDHFIVVPLVG
                     RLETVAVLIVDHFSSQRVVKPHLIESLKIFADNAGLALENVLNYDELRAKTLTLERQT
                     SLLNYHREFSQSILESLDVAIVVLDRENKIREWNRRAETLFQRAKEQVLGMGFDMLGP
                     AVFDLLNVADRVYEVKNTILLTKYEYSGKESKQYLDIKFTPLRSLEMARIEGIIIMFD
                     DVTQKKLLEDEIHRQEKLASLGEMSARVAHEIRNPLSVIGGFANRLINSIETEMKEKS
                     LRYLAILNSEVERLEGIVEEILEFSRPRQRLEFVEFNISDVIEDLRILYQDKAEEKGI
                     RLTTDIAGEMLFGDKRRIKQALINLIQNALEAGVTFVSINGYRQDAYYYCEIKNDGEK
                     ISESVVKNLFSPFFTTKVTGTGLGLPISKKIIEEEHGGKIFIHGVGDQKVKETVFVVQ
                     LPIENHPIGTT"
BASE COUNT         3407 a         3845 c         3764 g         3922 t
ORIGIN      
        1 ccgacttttt cctgttcgac catatcgacg accataggca gcgagttgat gttgatacag
       61 gcccatccga accccccgaa gatcagcgtc gcttgtatga gtgaaaaatc tgtcagtaga
      121 cctatgatac cattcattat taaaagaatg acaataccag ctaaaattgt cttttttctg
      181 ccatatttcg tcccgatgaa tccggcagga atggagaaga gcataaaggt cagtgagaaa
      241 aggcccaagg cgaaggaagc tttgctctct tcgattccga ggtggtattt cccgtagctg
      301 gtgaagaagg tttctatcgc gttgaagccg cagaaccaca agaatatggc gagaagtagc
      361 cagagttcgc tcttttcttt cgataagaac aactctttca gcgcggtcca gagttctttg
      421 aaggcttcta tgaaaccgat cttggcggtt tttttgctgg gtttttcaga agagtcgtcg
      481 gtttccgcga tcggctcttt cacgaaaacg aaaacgagca ggcagctcac aacgagggca
      541 acaccgccta tgatgaaggg aagactctta tttatttcgt agagttttga tcccacgaga
      601 aacgccagta gtgcgcccaa tccccccatg aagtttacca cgccattcgc tttgcttctc
      661 gattgtgatg ggaccgtatc gggcatcagc gcgatcacag gagcgcgaaa gatcgccatc
      721 gcaaggttca ttatcaagat ggtggtgatc atccaaccga tattgagcga tttggcgaaa
      781 gggatcaaca ggaagaaaac ggctgcgatg ggcgcgccta ccataataaa gggcatccgc
      841 ctgccgaatc gcgtgcgcgt ttgatcgcta agaaacccga tgtacggttg caagacgatc
      901 gccagaatgt tgtcgaaggt catgataaac ccgaccaaga gtgagctcaa gctaaactct
      961 ttcaggaata tggggatata ggagttatag atcgaccata acagagagat cgaaaaaaag
     1021 ccgaatccta acaaaaaggt cttgcggtag gagaacgtga tcttttccat agctcaactc
     1081 ctctatgatt ttgtaagtta ggccgcccga ccgtcaagtt ccggccggcg cttcttgcgt
     1141 cttgtggaag acatacccga tcaagcgttt gaaagacggt tcgacgggta gtgtctccaa
     1201 ttcctccgat gccgggtcga tccacaaaag attgagaacc catcggatga gttcgtacag
     1261 gttcgataag gttgggggcg catcaaccaa gtccgtccaa tcggaaaagc actcttgatc
     1321 gatgctcgaa atcctgctcg ccaagtccca atcgtactct tgagcggtta atgccagcgc
     1381 ttcgacggtt ttgtccaagt caaaggggaa accggcccga tgcgtgacaa gctcgaaaag
     1441 agaaaaacag aaagccatcc atcgtgtgga tctcgtgagg gccacgttga ccagcaggat
     1501 tcctcgaaag agttcgatcg ggtctgcgtc gtagttctcg aaaaggtcga cgatccagtc
     1561 caaaacgatg aggtcttcgc tactgtccat ctcttccgtg ggcattcggc cgacgagtag
     1621 tccgtgaagt ctctcctcga gatagcccgg tattccttcg taggcgcccg ctaattcggc
     1681 gaggaaagtg aaaaggtcaa aagggcgcga ggtccgtcgg ttatgaaaag gagagaaccg
     1741 ctcaacgtcg aacccgtgcc gcgtcaaaaa cggcagggag cggatcagta aatggtcgaa
     1801 ttcttccttc gatatgagca tcgtggtcca caaaagattc cacaggatcc caagattctc
     1861 ttcgtcggcc gagcgcgctt tctttagcgc atcgagcgat tcctcgaaga gtcccagctt
     1921 tcgatacagg acgcctattt cgtttaggac agggatcgag tctgggtttt gaaccagcgc
     1981 cgctttcagg tgcgccaacg cggtaaaggg tttcgatatt ttttgcaagg caaaagacag
     2041 gttgtaatgc atcggcacgt catcgggcgc gcgcgaaagc ccttgtgtga ggatgtgcac
     2101 ggctttcgtg tactgttggc ggttgttgta gatgacgcct aaacgggcgt aaggcggaac
     2161 gaagtcggcc ccggattgga tacaagcttt atagctgtct tcagcgcggt cgtaatcgcc
     2221 cgtccgtaga aagatttctc ccatagagaa cagggcgggt aaaaaagcgg gatcttcttc
     2281 cacgactttc gaatagactt ccaaggcgct atccacatcg tcttgttctt ccaacaaccg
     2341 cgccaattcc atcgcgaaat acggattttt cgggttctcg gtaacgattt tacgcaagac
     2401 cgtctccgct tggcctggaa agccggtccg aaaggcgtac aaagcttgat aaaagtctag
     2461 tctcgcgtcg tgcgctttaa cgagatgagg gaatcggtcg agaaaggccc tcgcttcctc
     2521 taaaagtgcg cccgccttgt cgaaagcctt ctcacttagg gccgatttca cgttttcgta
     2581 caagaaaaag aggaagtagg aagcgtagta gtccagagtc ccctcttccg atgtcgcacc
     2641 tttcgccgtt cccgccttta tctgagcccg caaccccctt aagaccatct cgggcctgac
     2701 ttccgaggcg gggcctaatt ccacaaaatc ttcgagcaac aaaggcaggc gaaggggtaa
     2761 gttcgagcgt ttggccattt ccggttttat ggaaaggtaa acgaggaagg tcttcaatcg
     2821 gtcttcccct tttcgtaaat tgacaataac tgatccacct cgatcatcgt cagcaagatg
     2881 tgaccgctgt ccatcagcaa gatcgacttg gtttcatttc cataggttaa attgatgacc
     2941 tttcgttctt ggtcggcgac cattttgatc tttttcatcg cggcgttctc gggagaaaaa
     3001 acggcttgta tgcgatcttt cgtgacgaaa gcgtccgccc cgagtgagat gaggagatcg
     3061 ttatcggact tcaaatctcg actcctttga ggtatttcgc cgcgtcttcg ggaggggtcg
     3121 gattgatata aaacccggaa ccccattcga atcccgcgac cttcgtcaaa cggggtacga
     3181 tttccaaatg ccagtgaaag gactgcttcg cctctcccgt cgtgggcgcg gtgtgcaaca
     3241 ggaagttgta ggggggattg tccaaggact tatagatccg caagagcgat tttttcaaca
     3301 ccgtcgcgaa ctcttgcacc tgcacttctc ctatctctcc gaaatcgtgg ctgtgattct
     3361 tcggcagaat ccaagtctcg aaaggaaatc gtgaggcgta cgggttgatc gagatgaatt
     3421 ggtcagtttc ttcaacgatc cgtacgctct ccatccgttc ttgatagatg atatcgcaga
     3481 atacgcaccg ttctttgtat tggaaatagg tttgggcacc cgtcagctct tcttgcacgc
     3541 gtttgggtac aatcggagtg gcgatgatct gcgagtggga gtgggcgaga gaggcgccgg
     3601 catccctttt gtggttttta aagatcaata tgtattttat acccgggtct ttcgccaacg
     3661 ctttgtatct ggctttgtac gcccagatca tctcttcgat ctgtttgcct tcacaggtcg
     3721 cgattgtggc gttgtggtcc ggcgtttcga tgacgatctc gtggtaaccg aaaccggtca
     3781 tcgcatcgta catcccgtgc ccatagcgtt gcatcttgag atttggttct agcgccggga
     3841 atttgttcgg cacgacacgg atccaccagc cgggcaggtt gggttgagaa tccttcggcc
     3901 tgaaggcgag gatttccggc ggagtcgtgt actcgttccc gtaatcgaac gggcagaagg
     3961 cgcccttcgc ttcttcgggc gcttttccaa agtccatcgg ccttttcgcc ctttccgtgg
     4021 cgatgatgac ccatcggttc gtgatcggat cctttcttag ttctggcata gggatatgcc
     4081 ccccttttta caaagcgcgc ttatttttaa ggccttccgt gtatatgtaa acgtattctt
     4141 ttgcagaccg ttcccaggag aaatcgtccg tcatcgcgtt tttaatgaga gtctcccaag
     4201 actctttgcg tcggtaaaag tagagagccc gaaagatgct gagaagcagc tcgctttctc
     4261 tgtactcgct gaatccgaat ccatttcccg ttttctcttt ttcgtcgaat tcgatgatcg
     4321 agtcggctaa cccccccacg tagtgaacga ccgggatcgt accgaaacgg agactgaaca
     4381 tttgtcccaa accgcacggt tcgtaacgcg agggcattaa aaagatatcc gacccggcgt
     4441 agatgaggtt ggccaaatct atgtcgaatt ttaggttagc ggaaactttc ttcgggtttt
     4501 cagcggccat ttcttgtacg agctcttcat attcggcgtc tccggtgccg aggacgacca
     4561 attgcacatc ttgcatcagg aaaaacgggg tgatcttcga cagaatgtcc agcccttttt
     4621 gggcgaccaa cctcgagatg aggccgaaaa ccgggacatc gggattcact tccaaaccca
     4681 agcgctcctg gagtaaccgt ttattgcgag ctttacccga taggtcgttc ggggtgaaag
     4741 cgtaaggcac gagattcgtt ttggtgggat cgaagatatc gtagtcgatt ccattcaaaa
     4801 tcccgtataa ggcgtcgctg cgtatactca gaacgccctc gagtttttct ccaaaggctt
     4861 tcgtttgtat ctcttgcgcg tagcgagggc tgacagtcgt aacgatgtcg gagaataaga
     4921 ccccgccctt caaaaaattg atttcgccgt agaactccag cgcgtcgacg ttgaacaggt
     4981 aatgcggcag accggccata ttgaggtaca tgctgggaaa cttcccttga taccccaggt
     5041 tatgaatggt aaaaacggtg agggccttct gaaacatcgg ctcctcgcga aggagcgtct
     5101 ttatatacac cgggatgagt cccgattgcc agtcgtgggc gtggatgacg tcgtaggatt
     5161 tgcctaagac ccgaatggcc tccagagccc caacattgaa aaagatggcc tgctctcctt
     5221 ggtcttcgta gccatacact ttctcaccgg aaaagaaatg gtcgttgccg atgaagtaca
     5281 cgttgacatc ggaatcgggc aggcgcgaac ggtataattg gacctcctgg atcgtttgca
     5341 taaaaggaac ggggaagggg cttccgattt tttctatgga atagccgaaa ggtttggcgt
     5401 ttttggatac cgctctgtgg taaggcatgt aaatgtcgat ttccataggt tgcgagcccg
     5461 aacgcgagct caggcgctcc aagtattttg gtagggatcc caagacgtcg gctaatccgc
     5521 cgacttttac aaatgggtag acttcgtagg acatcatcgc gattctcatt ttcgatcacc
     5581 cctttagtag gcttctcgtt tcttcaggta attgataggc acacgtattt tttccaatat
     5641 ctccttcact tcggagacgt tatttgagtc aattctcaaa tacacttccc gaaaggcttt
     5701 tccgcccgtc gacaaatgct cttttttgta agtcagtatc gagagaatgt tgatattata
     5761 aaacgcgagg aggtcgacca gggttttcag tttgcctggc gtatcttcca acaataagga
     5821 tatcgacatc ccgccttcgt cgaatgccgc gacctgggtg aaagcctcca gtatttcgaa
     5881 gatcgtgatg caccctaaca gacgcctctg gctatctaaa acggggacga gcatctcgtg
     5941 gttttccatg agcagaagcg cgacgtcttc gagcgtatcg tcctcttcta cgaaaaactc
     6001 ggggttgctg ctgtaatcga tcgctttttc cgttttttcc gcatccttaa agtcttcgta
     6061 gtgaaggatt ccgagataaa acccgcgctc gtcaaccacc gggagagagt tgacgtcttc
     6121 gttcgaaaaa aacttcttga cgtcttttgg gtgcatatca ccgaccagtc tgggtttttc
     6181 acggttgtac cactctctaa cgaacacacc aaacaccccc atcagaaaga gatcgtttgt
     6241 acccgtcggt tcgcgcttat gcacaagtcg cgcagaaaaa ggcgtttttt ctcagccgat
     6301 cgtcctcaga tagttcataa tgcctttcgc ggctgttttg ccggcgccca tcgcttcgat
     6361 gaccgtcgcg gctcccgtga cgatatcccc acccgcaaat actcccggga cagaggtcat
     6421 caacgtttcg ggatcgactt cgatataccc ccatcgattc agccgaacct ctttgaagcc
     6481 ttccgaaagg atgcggtgag cggattggcc gatcgcttcg ataacgcaat cacattccag
     6541 ggtttcctca acgccaggca acggaacggg tttgcgtcgc ccggaggcat ccggctctcc
     6601 caatcccatc ttttgtattc gcgctccttt gacgcgagag tccgagtcag gaaggtatgc
     6661 aatcgggttc gacaaccata agaattcgac accttcttcc atagcatggt ggtactcttc
     6721 ccatcgcgcc ggcagctccg tttcagttcg gcggtagacg atcttcacgg tctgggcgcc
     6781 cagccgcagt gcgctcctcg cggcgtccat cgcgacattc ccgcctccga cgacaattac
     6841 gtttttccct atggtgatcg gcgtatccgc atgtgggaac cgaaacgctt tcatcaggtt
     6901 gacccgtgtc aaaaattcac tcgccgaaaa aacaccgttg aggttggttc ccgggacatt
     6961 cataaacttt ggcgctcccg ccccgattcc caaaaaaatc gcctgatacc gttttcgtat
     7021 ttcctcaaaa aaaattgttt ttccgactat aaaatctgta cgaaggacga cgtccagctt
     7081 ccgtatcccc tctatttcag cgcggacgat ctctttgggt aaccggaact ccgggatacc
     7141 ataggtcagc actccgcccg gttcgtggag cgcttcgaac acttctaccg cgtagccgtt
     7201 ttgcgcgagt tcggcggcga ccgtcagacc cgcgggaccc gatccgacaa ccgcgatccg
     7261 ctcggtatgg gtgagttcga cgggcgcggg agagaatccg gctttccgtt tccaatcggc
     7321 ggtgaaacgc tcgagcctgc caatggcgac aggttcggag tttttaatct tcccgacggt
     7381 acaacgctct tcgcattgtt tttcttgcgg gcacacccga ccacaaatgg ccggaagtcg
     7441 gttggtcgcg tagagtatct cccaagagcg ctccaaatcc ttttcttgaa tggctttgat
     7501 aaaacccgga atatcgatgg cgacggggca cccttggatg cacggggcgt ttcggcattg
     7561 gagacagcgg tctgcttcgg agacggcctc tgtcaaagag tatccgtcgt tgacctccag
     7621 aaagtcttta cgcctttcct ccggagcgcg ttcgatcaat ttggtcttct gtttggaacg
     7681 gttcagcata cgcggcctct ccttctcatc gagaatgcaa ctggttttct aaccggcaat
     7741 ggtgaaacga atccgcttcc tcggttttga actgtttctg ccgaaggagg aggttatccc
     7801 agtcaaccag ccttccgtcg aattcgggcc catcgacgca cgcaaaaaac atttcgttcc
     7861 ctaccaagac gcggcatccg ccgcacatcc cggtgccgtc gaccatgatc gggttcaaag
     7921 aagtccagag cgggaaatcc aatcgtttgg ctatttcaga acacatcttc atcataatcg
     7981 tgggcccaac agcccagccc acatcgtagg cgccggtgtt ggcgacgagg tcgttcatcg
     8041 cgtccgtcac gaagcccttg cgtccttcgg atccatcgtc agtgcagagg accaggcggt
     8101 gagcgatcgg tttgaactcg tctttcaaaa tgagtaagtc tcgggttcga gcccctagaa
     8161 cgacggtcac gtcgttcccg gcatgatata agctcccggc gatcggtaga atcgcggcga
     8221 ttccgacacc gcccccaatc accaacacct tctttccttc gacgacttcg gacggcttcc
     8281 cgagggggcc tgcgaggttc agcacctggt ctccaggctt cagaaggctc agcttatggg
     8341 ttgaagtgcc aaccacttgg aagattaggc ggacggctcg ctttttcgcc tccgtttgtg
     8401 caatcgttaa cgggatacgc tcaccctcct cgtcggctct gatcattacg aattggccgg
     8461 ctttcgcgtt tcgtgagatc aaggccgcag agccttcttc ggaagtggaa acgacggtca
     8521 tatcgtatat ttttggcgcg attttcgtta tggtcgatac gatctcttgg ggtggtttga
     8581 tcggtgaggt catcctttct ctccttatgt caatctattg gcataggttt gctgtcggat
     8641 gatctgcgcg cagcgggggc agatatccgg gtgaatctga tcttctccaa tcttgaggtc
     8701 atacttccaa cacctttggc atttcgctcc ctgagcgcgt tgtacaccga tctcaagagg
     8761 cgaatccgcg ttttcgacga aggtggtctt gcattctgag acgatgaaaa aatcgcttaa
     8821 aaacgcgtgt tcttcttgaa gcaatcgata gagttcggga tctttcacag agatggatac
     8881 ggaagcgtcc aaagaatggc cgatgacgcc tgccgtacgt actgattcca aagaagcgtt
     8941 cacgcgttcg cgaagctcca gcaaccgttc ccatttttga ttgagtgtca gaccgttaga
     9001 gccgatgtga tttcctttcc actccggatg tcggatcggc caagattctg cttggatcgt
     9061 ttttttctgt ttcaacgccg ccgggaagaa ttggtaaacc tcctcgctgg tgaaggtcaa
     9121 gatcggggct aataggagcg tgagcgtttt caaaatttct agcagaacgg tttgcccact
     9181 ttttctcacg ggggagtctt tgccctccac gtagaggcgg tctttcagaa tatcgaggta
     9241 gacggcgctc aggtccgaaa tcacgaactt gttgatcgtt tgaatgccct tgtaaaactc
     9301 gaaggtatcg aacgactcag ttaccgtctc cacaagatcg ttcaatcgcg aaagcgccca
     9361 gcgatcaaaa gagctgagtc ggtcgtatgg aaccgtttcc ctttccggca cgaaatcgga
     9421 gaggttgcct aaaaggaaac gcatcgtgtt gcgtatcttt ttatagacat ccacctgctg
     9481 gagaatgatg tccatagaca ggttgatatc gtttcggtaa tcagccgtcg cggtccaaag
     9541 tctgagaatt tcggcgccat atttgtcgta tacttcgtga ggtacgatga catttccgag
     9601 ggatttgctc atctttatcc ctttgccatc ccgtatgaac ccgtgagtga cgacttcttt
     9661 gtagggcgca atccctcttt ttgcgacgga taggaacagc gaagactgaa accagcctcg
     9721 gtgttggtcg cttccctcga gatagagttc ggcggggaac cccaattccg gatctgccgc
     9781 cataaccgac tcgaaggaag agcccgagtc tatccacaca tccaatatat cttcttcttt
     9841 ttttaggttt ttacctccgc aatacgggca acgaaaccct tccggaagta ggctttcggg
     9901 cggttgctcg aaccaaacgt ccgttccttc cctctcgacg atcggaatca ggtgggagat
     9961 ggtctcctca gtcatcaccg tatttccgca tgattggcaa aagaacgccg ggattgggat
    10021 tccccaaacc cgctggcgcg aaatgcacca atccggccga tctcggacca tcgctagaat
    10081 gcggtttttt ccccaatcgg ggacccagtg gacatggtca atttgatcta gggctttttc
    10141 gcgtagatcg tcgccgtcca cccgaatgaa ccactgttcg gtggcgcgga acagaacggg
    10201 ctttttgcac cgccagcaat gagggtatgg gtgcttatat ttttccgacc atatcagatg
    10261 accggactcc ttcaggtgct gaacgatggc cggattagcg gagaagatga actggccttc
    10321 gtagtccttc acgtcctcgg tgaactttcc ctgagaattc actggagaat atatatccag
    10381 cccgtatttt aaacccgtca gataatcttc ctgtccgtgt ccaggggcgg tgtgaacgca
    10441 cccggagccg gtttcaagat cgacgtaatc ggcgagaacg atcagagaat cgcggtcgat
    10501 gaaggggtgg cgggcgcgct tgttttctag ttgcgtgcct ttaaccgtcc ggaggacacg
    10561 ataccgagat cgatttgtct ttttcatcaa cggctcgacg agtttttcgg ccagcaccca
    10621 cgtttctcca tctatgtcca cgtaagcgta gtcgaaatcg gggtgtaccg caatcgcgag
    10681 gttagcgggt agtgtccaag gggtggtcgt ccagatgacg atataggtgt tctccgcacc
    10741 ttcgagagca aacttcacgt agatagagtc ggaagtgtct tccgcgtact cgatttcggc
    10801 ttcggcgagc gctgtttcac aactgtaaca ccaatgaacc acctttttgc tgcgatagac
    10861 gtttcctttt ttcaccagtt ctcggaaaac ctcatacacc ccggcttcat aagcgggaag
    10921 aaaggtaaga taggggtcgg cccacttccc gcgtatgccc agacggataa actcttccct
    10981 ctgcaagtct acgtactttt tcgcgtacgc ttgacaggct ttccgtattt ccatcttatc
    11041 catctttttg cttttgtcgc caagttcggt gaacacgcgg tgctcgatcg gtaacccgtg
    11101 cgtgtcccat ccgggtttga aaggcgattg atagccctgc atcgtccaat acttgacgat
    11161 acagtctttt aaaattttgt tgagcgcggt tcccacgtgg atttctccat tcgcgtaagg
    11221 cgggccgtcg tgcaagacct tcttcgggga attcgcccgt tcttcggaga tataggcctc
    11281 caggccgatc tcttgccaat aacggagaat ttcgggttct ttttgcggaa ggttagcccg
    11341 catcggaaaa ctcgttttag gcaactgcag cgtatccttg tattccatag tctattctat
    11401 tcctcccgtc ggtttagaaa acacgagacc taacgaacag attaagcgtt cgttgcgttc
    11461 agcagttcct tgatcgtgct cttcagttcc aagaaatttg agctttttac gatataggcg
    11521 tcggccgccc aagaagacag atcggatttg tagtggctgt aggcggtgag aagtatgatg
    11581 cgaacgggga ttttccgctc gcgcaagagg ccagccaact ccaatccgga catatcgggc
    11641 atctctatat caatggtcac cagatcgtag gatccctctt cgaattttcg aaggcactcc
    11701 gatccgttcg cggccgtatc gacgaggtac ccttcatcgc tgagctcctc ggagattaaa
    11761 aaccgcatat tgtcttcgtc ttcgacaacc ataatccttg gtttttccat gtcattcctc
    11821 ctattctcat aggtatttaa aacttttcga ctaagtcgtt ccgattggat ggttctctat
    11881 cggcaattga acgacaaaca ccgtttcttt gaccttttga tcccccactc catggataaa
    11941 aattttcccg ccatgctctt cctcgattat ttttttcgag atcggcagtc ctaaaccggt
    12001 acctgtgacc ttggtcgtaa aaaagggaga gaaaaggttt ttgacaacgg attcggaaat
    12061 cttttcccca tcgtttttta tctcacaata ataataggca tcttgacggt atccgttgat
    12121 gctgacgaac gtcacgcccg cttccaaagc gttttggatc aggttaatca aggcttgttt
    12181 aatacggcgc ttgtctccaa aaagcatttc acccgcgatg tccgttgtca aacgtatccc
    12241 cttctcctcc gccttgtctt gatagaggat tcgtaggtct tcgatcacat cgcttatgtt
    12301 gaattccacg aattccaaac gctgcctagg ccggctgaat tccaatatct cttcgacgat
    12361 accttccaaa cgttcgactt cggaattgag gatggcgagg tatcgaaggg atttttcctt
    12421 catctccgtt tcgatggaat tgatcaatcg gttggcgaaa ccgccgatga cagacaaagg
    12481 gttccgtatc tcatgggcaa cgcgcgcgga catttctccc agggaagcca gcttttcttg
    12541 acgatggatt tcgtcctcca aaagtttctt ttgggtgaca tcgtcaaaca taatgatgat
    12601 accctcgatt ctggccatct ccagggaacg taacggagtg aacttgatat cgaggtattg
    12661 tttgctctcc ttccccgaat actcgtattt cgtcaacaag atcgtgttct tcacttcata
    12721 gacgcgatcc gcgacattga gcaggtcgaa aacggcgggg cccagcatat cgaagcccat
    12781 tccgaggacc tgttccttcg ctctttggaa aagcgtttcc gctcgacgat tccattcgcg
    12841 gatcttgttt tcccggtcca acacaacgat ggccacatcc aaactttcta gaatactctg
    12901 cgagaactcg cggtggtagt tgagaagcga ggtttggcgt tcgagggtca acgttttcgc
    12961 ccgaagctcg tcataattga gcacgttctc gagggctaag ccggcgttat ccgcgaatat
    13021 tttcaaactc tcgatcaagt ggggtttcac cacacgttga gaagagaaat gatcgacgat
    13081 caaaaccgcg acggtttcta gtcttccgac gagcggaacg acgatgaagt ggtccgtctc
    13141 cagcaagttt ttcaattcac gatactcgtt accccactcc cggatcatct ccggagtaac
    13201 ttggacgatt tttttgcgta agaccgcttt ttcaaagatg gcgtggcttt tgtagggaaa
    13261 gacgcgattc cgaagtttgt ccgactcata ctcctttagg tcggtcataa tcgccttctc
    13321 tctcaaatgc tgagaaaggt tgccatattt gcccgtggcc gaaccgcttc ctcgcgttcc
    13381 ctcttgttcg cggcgggcca tccaatagcg agggatcaac gtttgactct ttatatcttt
    13441 ggcgagaagc atcgcgcggt cgaaccccag cccggcgtat tcggtggctc ctaacagcag
    13501 gatcgtatag atgttctgta tatcatagct ttcacgcatt gcgcggttga tagcggtgat
    13561 gttttttagg aggttaacct gctctttttg cgtttcgagc aaactttcgt tggaggcgct
    13621 caattcgctg agttccttgt aatctttgga tagctggttg tagtatgtca ttttggaaac
    13681 gatcaaagag atacgcgcgg cgaagtcctc caacaaagcc aagtcaaact cgtcgaagtg
    13741 tttgtagggt cggtagagct cttggtcttt gcggttgatc aagatgatga caccgaagat
    13801 ctcgttttcg tggaagagtg ggaccgctat cgacgattta atgtcgacag ccagcatatc
    13861 cagggctgcg cgcgggtctt tcttcacgga gatggacagc atcggcttct tatgtttata
    13921 gcaccatccc accataccgt gcccgactgg aatcgtgcgt tttaacagtt ggttctcgtt
    13981 taagccgtac cagtagccga tctccagatc cccctcggca ttagccaacc agatcgaacc
    14041 gttttccaca ttgagcacgt cgcacgtctt tttaagcgag cgttcataga gttcggagat
    14101 ggcctcggtt tttgtcacga cgtcgctcaa tcgcgttaag ccgagcaact tccccacgtt
    14161 ttcgtagagc aagcgattct cgatggacaa gcggatgatc ttccccagaa tacgaaagaa
    14221 atcataattc tttatatcca gggcctgaag cttttccgga gactcatccc ccaaaaagat
    14281 gaggctgtct aagaggccgt catcgaggat cggacaaatt agtaggtttt ctgattcgaa
    14341 tttacgattt tcgtcttcta gcgccttttt ttccaagaag acggacgagc cgcgttttag
    14401 gtcttggata gaagggactt ggaattcttt gatcttgaac tgcttaacga aacgtcgatc
    14461 cagaatcgaa gtgcctatca atcggaaggc gagtttttct ttctcgtata cgaagatcga
    14521 acactcctgg acattcaaca aagccccgat ttcttcagcg agcaggttga cgccttcatc
    14581 ggagtgaacg atatcgaggt tgagtagggt atggatgatt ttgatcattc gattctgttg
    14641 attcaatttt cccctgttcc cttcggtagc agtctattac cacggtttcg gcgcttccta
    14701 aaattatacc attttttcgc tgaaatagga agagaggcca actgcaaaaa acggtcgcgc
    14761 gccaagtgat gaggtgctat caagaaaaaa ggccagaatc gaccggacgc tttttatgtc
    14821 gttgtagtca tcgcgtcgta agcgcctctt cttttcgtgg gggcgcgata cgcttttgag
    14881 ggagggcatc gcggcgtaag cgccccttct tttcgcgacg gcgcgatacc cctttcat
//
LOCUS       DAOPXN010000048        14838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4114_length_14838_cov_5.777650, whole genome shotgun sequence.
ACCESSION   DAOPXN010000048 DAOPXN010000000
VERSION     DAOPXN010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14838
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_14838_cov_5.777650"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PKM49_04080"
     CDS             <1..913
                     /locus_tag="PKM49_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNY82025.1"
                     /translation="VLDILRSHGKKVISIRHPMPYGDLVKQKVQRFANYSDLDTHDCT
                     IEEREEYEPHIDRKSVIYAGVDYEAILREAEKEQPDVILWDGGNNDFPFYKPDLWITV
                     VDPHRAGHEYSYYPGAVNTLAGDVIIINKEETANPEDIQQVRLRVQSLNPNATIIDAA
                     SPIFVEKYQQIKGKKVLVVEDGPTLTHGGMKYGAGLVAAQKFGAGEIIDPRPYAKGSL
                     LSTYKKFNHLDNILPAMGYGAKQMKELEESINATPVDLVIIGTPIDLTRVIKINKPAL
                     RVQYELQEIGKPDLEDVLQDFIRKHNI"
     gene            2082..2258
                     /locus_tag="PKM49_04085"
     CDS             2082..2258
                     /locus_tag="PKM49_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82026.1"
                     /translation="MKKKGLWIVAIGAVVLFCSGCFIQDTISYIQSVEDIVNTVYVIG
                     IVGFVAGWLLFHKE"
     gene            2390..3514
                     /locus_tag="PKM49_04090"
     CDS             2390..3514
                     /locus_tag="PKM49_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY82027.1"
                     /translation="MSKRVFLLSFLILITVSVGLSFSVWVSWEGEDWYRMIAKAYTEA
                     TQTPVEIVPIPAPDQKLLVALKTPDTLPDLCLIKSDDFPLAATIALPIDLPRLPQYAD
                     RFAQIGIQTYTSGGALRAVPFYADTQVLFLQTAVFEKYRIPLPEPDWNIGDFEALLAH
                     LRAEGVMPIGWGFNSPYIFQGLQEGFGQSFLPDGTIRVITEENTRLLKRMRSWVTNGW
                     FKDYPQRPGLVKDFSAGKVAMMPQGSYLIPDLDEMGVPYALAPLPEPWRSVIDTKAWV
                     IFKNETLVYPFLSFLFERMEGLCATYAKQSLIEGIPFSGNAGGVLERALENGLVQPST
                     RAYSEGYWTAMKACLELYLRGEGELQELLSTAQAYIDQFQ"
     gene            3511..5361
                     /locus_tag="PKM49_04095"
     CDS             3511..5361
                     /locus_tag="PKM49_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82028.1"
                     /translation="MTFFLPFFLLIAGMTLFPAVFSAIAGQAQIRFDLFWTLFQDGAF
                     RISAQVSALYAFIATLSVFFCGTTLAYLLIRLRRFWRKAVALLLFVIWALPSFVSLPI
                     FRALLLNTIENPIASEIQAFCSILVGRFWVDLPVVALIALALFEELPPSYEESMRLEG
                     AGTVDRFFSLFFQIAQKSLVGYLCVSFLDLMRDVNVPMMITEGRPLSYAGFTPYGIAG
                     ATTTLGFFLKNNLLDFAATDSWTFLWSQNALVTVTLLAGFWFLNRMFKRPEKTFGIWL
                     GLEIVWSFSLGSLLFLPAFFFRDWHRRGHLRACAIIALGASLILRQLSPSTVILCFYL
                     LFRLFPFQRAFDRLLFFFNVALAVLWIAMSGGALGFLVWLSFSRYRYLPPFPGIADAF
                     KGLSWNNYVYLFDSGFLVNLKNSLWLGLGSGLLGVLVFSMAAYAALKNSRYHTFAKGL
                     IGLTLVMAGMNTFVPLVLTLDALGGLNRFLPIVLAILNQSAPIAFMTAYAAFRQTDPT
                     YAELGQIDGASRWQLFWKIVFPLAFPTVAVLFLYIFIRGWSSFIAPLLLISSPEKYPV
                     SLRLYDWAGNPGLHHTRWGLFAAGALLTVTMMAVCSIPLPALWRKRWSSG"
     gene            complement(5394..6578)
                     /locus_tag="PKM49_04100"
     CDS             complement(5394..6578)
                     /locus_tag="PKM49_04100"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HNY82029.1"
                     /translation="MQDWMELTNEMEEAKEQGLYTWIRTLEGPQGAWLTIQSKKVLNL
                     SSNNYLGLANHPAMKQAAIEAIQNWGVGPGAVRTIAGTMSLHNQLEERLAAFKKVEAV
                     IMLQSGFTANQAVLAPLVGAQDALLSDELNHASIIDGTRLTKAMRFVWKHKDMVDLRE
                     QLRKAQLEGARRKIIITDGVFSMDGDIAPLPEIVALAKEFEAIVLVDDAHGEGVLGSH
                     GRGIVDHYHLHGEVDIEVGTLSKAFGVIGGFVAGKKPLIDYLKQKARPFLFSSSLSPA
                     DTAAAIESVKILESSDELVKKLWENAEYFRSNMTRLGFDTGHTQTPITPVMLYEAPLA
                     SQFSRTLFEKGVFAQSLGFPTVPKGKARIRVIMSAAHSKEDLDYGIRAFEEVGKELGV
                     LR"
     gene            complement(6550..7743)
                     /locus_tag="PKM49_04105"
     CDS             complement(6550..7743)
                     /locus_tag="PKM49_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82030.1"
                     /translation="MSWPSPTPVVRSSGAMLPLAAFSIEKERLLKIVSYCEMIAGSEK
                     KYGFFWLFHHQGLRIFVTDGSLRTVFQAKGEYPAFERAYQFPIRPLKLFLQERSVDTH
                     VNVLLGEESAIFRCDNEVLSLRQGQDRRIEDLSFPDSLCTVPFKAFMNGIDFATISLP
                     MLDRVFLVDYDGQFAVVSVEQDSFALFNSEIPIRRFEDGSFLKAQIPYASIRHFVKAC
                     SLIDVNELKIGVSLESEEIYFQTAGAVTALCMKALDERDETDLFRLLHVYERMEPVAR
                     VDSGKARKLFSKALRLSDNGILNLSRYPEELEFWISKKTLYYSVRDSLEPYCETADAP
                     SSIQFYGKMIKSSLNRITTKKLCMFQSGEFIGFGDENKRRMAVVRGKKVERSWNHAGL
                     DGTHE"
     gene            complement(7740..8462)
                     /locus_tag="PKM49_04110"
     CDS             complement(7740..8462)
                     /locus_tag="PKM49_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82031.1"
                     /translation="MLYKAFEVLENAAGHTCGHWKIHLKKTIPTQSGLGGGSADAAVL
                     LRFLCRRFGLDPHTEWSVAKRLGADVPFFLLCGTAIGEENGSALSPLPPLPALPVLLV
                     QPPYAMSTAKMYEQYDQMVSRSPNHPLREDRSDQQTAQDVYMALAQQLPVISRNDFEE
                     VALTYYPEYRVFHSFFSRLGEDGMHRDPAILLKRMSGSGSAHYVVFKADTDPSVLARY
                     ESLCRDAGCWATLTRFSRGDVV"
     gene            complement(8693..10927)
                     /locus_tag="PKM49_04115"
     CDS             complement(8693..10927)
                     /locus_tag="PKM49_04115"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY82032.1"
                     /translation="MKTTLTLVVLCICLTLTAFSQDLFELKSVQSKILLAYDLNDYSL
                     LDAAIIDLQKIILDRGSDPYVLSKAHLLTGDAYFYKGYYSTAIEAYEKALQYTGKDDQ
                     DYPYAVYSKCYATYHMAMETHQSQRDSLLNQALILLEQLVSFSGYQQDYYLLKGMIQK
                     AKGDYLAASSSFDAVTDPSAIGFASYYKGLIFFEQGNYPSAIMAFERAKQFGQSPELM
                     AASIYQTIRAMMNLQNYREALSYSENLVRNYSTTRYRDEIFMLHVELLYRTGEYANAA
                     RYIDYIIENAKSTRDLVDAYNAMGWLAYKSGNYETALANWNKAISVGAAEYPIETFEV
                     AKQAINVLRNTGDSAKMVSYLNQMKATFPSQGTALDLESAKVYLSLGRYSEAEVLLQR
                     VLSSGLFYQDANYTLAQLYQKTGDMKRAQDYVDRVIRSGNQAAVFQGYTFQGDLYLQT
                     GDYQKAKIAYENALKNASEAERSQAILNLGIVALNAKDYATAQKQFETLKTEITKDLS
                     AALDAAFYMAETYIARNLPASALNEYDWILRNDVSKKYYKTVTLKKYTVMLDTNQDPL
                     EIARQIDAALQVTSDSALSAELRYLRAEAYLKKGDLISAFEALRDASFDRLSAGSKGG
                     YLYIRGKYYASQGNEGEMRAAFEQLIDQYPQSPKTPWAIQDYALYYYNKNDYTNAKNQ
                     FFKLITSYPDFSKNDTAYYYIGLCYEKSGERDKALKIYQDLVAKYPASSRVSEAKKRI
                     EALQ"
     gene            complement(10941..11687)
                     /locus_tag="PKM49_04120"
     CDS             complement(10941..11687)
                     /locus_tag="PKM49_04120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNY82033.1"
                     /translation="MTLQYYLHRCGLAARRKVNAIIEGGRVRVNGVVVRDFWAPVDPE
                     KDRITVDGAVCALPLGHRYYLFHKPSGLITTVSDPQGRPTVIDYVRRVHGLSGPLHPT
                     GRLDKETEGLLILTDDGDFTYRITHPSHEIPKVYLAVLQSEIGHEQILLLESGVVIDG
                     VKTAKAKLSETRLEKRGFTGRPCWRITIHEGKKRQVRRLFESVGSRVVYLRRDQIGPF
                     TLEGIEIPGQIREIDENELEDFRKAYFNDG"
     gene            complement(11689..12354)
                     /locus_tag="PKM49_04125"
     CDS             complement(11689..12354)
                     /locus_tag="PKM49_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82034.1"
                     /translation="MRPRLCAFLLLSLFGLSVFGMLFEIGTGYRFAPFSFANHGWNLD
                     LGLFNQFNDMLTLDVTYHPQFSFSESGEREATPTNYLNFDVDVMIPLLGRRETREGYR
                     FGLFGTFEFGNFYDTYKDPSETSSDLSNWEVWQNSGYWLGAGLYGQYYLHPWMFQLGI
                     GFPIIRSPNTLNTMDWLLGTVEFKTRFFIESDRHRFRDHLTVEIEISARKIGLSVCLI
                     EPF"
     gene            complement(12351..13436)
                     /gene="dprA"
                     /locus_tag="PKM49_04130"
     CDS             complement(12351..13436)
                     /gene="dprA"
                     /locus_tag="PKM49_04130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNY82035.1"
                     /translation="MERIERLALRGSKIFTPAEIDTVEAFSSISEFLKSETAPMLERK
                     KRRVEQMWNADIADRCRKVSGRKDLTIFFPEEEGYPPLLREIDPMPQVLFIKGQAEWL
                     SSPELKIACVGSRKPSSYGKYVVQKIIGQLAERRVTIVSGLAYGIDVMAHLTAIEKGT
                     HTIGVLGFGVDHIYPASNTVAFRKVEEQGCLVSEYLPWQKPEVYFFPERNRIISGLSQ
                     GVLVIEASKTSGSLITVQHALSQNRDVFAIPGSIQSALSEGTNGLIKKGAKLVTDALD
                     IVEEYPWLVPDTLASPAAGADSASLASEERAVYDLIVEGCDQFDSLAERTRYQASTLN
                     RILTGLSLKGLVTKIGSQYLPGREGFV"
     gene            complement(13449..>14838)
                     /gene="hslU"
                     /locus_tag="PKM49_04135"
     CDS             complement(13449..>14838)
                     /gene="hslU"
                     /locus_tag="PKM49_04135"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNY82036.1"
                     /translation="YIVGQTAAKKAVAIALRNRIRRQKLLDPLRKEVIPKNILMIGPT
                     GVGKTEIARRLAFLSGSPFVKVEATKFTEVGYVGRSVDSMVRELVESAINFLKKEEMQ
                     KVVALAQKEVEKIILNAITKTSVKQPEKKLGDFFSRFLDSKITMESFGDRDRQTSQKA
                     ETFEEDTPPPHPMDLAERYRHGELDDQFIDIEIEKAQQPPLGVMGLGGDMSDLEIDLQ
                     GVLSNLMPPKKIKKRMTVAQARKILMPIESEKLIDTDKITSDALELAQNDGIIFIDEM
                     DKIASSSKSVSGPDVSREGVQRDLLPIIDGTTVMTKYGIVRTDHILFIAAGAFHISKP
                     SDLIPEMQGRFPIRVELDPLTERDFERILLEPECSLVKQYKALLETDGVTLVFDDTGI
                     HEIAHLSFELNERLENIGARRLYTVLEKVLEEISYDAPDTDNTYRMIDGDYVRSVMSP
                     IVSDQDLSSYIL"
BASE COUNT         3233 a         4180 c         3779 g         3646 t
ORIGIN      
        1 agtcctcgac atcctgcgaa gccacgggaa gaaagtgatc tccatccgtc atccgatgcc
       61 ttacggtgac ctcgtaaagc agaaagtgca gcgtttcgcc aactactccg atttggatac
      121 gcacgattgc accatcgaag aacgtgaaga gtacgagccc catatcgata gaaaatcggt
      181 catctacgcc ggcgtcgact acgaagccat ccttcgagag gcagagaaag aacagcccga
      241 cgtgatcctt tgggatggcg gcaacaacga ctttcccttc tataaaccgg acctttggat
      301 taccgttgtc gacccccacc gcgcgggcca cgaatactcc tattaccccg gcgcagtcaa
      361 caccctcgcg ggtgatgtca tcatcatcaa taaagaagaa acggccaacc ccgaagacat
      421 ccagcaagtt cgtctgcggg ttcagagttt gaatccaaac gcgaccatca tcgatgccgc
      481 gtccccaatt ttcgtggaaa agtatcaaca gatcaaaggg aaaaaggtgc tcgtggtcga
      541 agacggccct accctcaccc acgggggtat gaaatacggt gcgggcctcg tggcggcgca
      601 aaaattcgga gcgggtgaga tcatcgaccc caggccatac gccaaaggat ccctcctatc
      661 gacctataaa aagttcaatc acctcgacaa tatcctaccc gcgatgggtt acggcgctaa
      721 gcagatgaaa gagctcgaag aatccatcaa cgccacgccg gtcgatctgg tcatcatcgg
      781 aacgccaatc gacctcaccc gagtcatcaa aatcaacaaa cccgcgctcc gagttcaata
      841 tgaacttcag gagatcggaa agccagacct ggaagacgtt ctgcaagatt tcatccggaa
      901 gcacaatata taagcggccg ttcggctcga caaggtgagg gtataatatc ggggtacata
      961 aaaatgggaa cgccggactt tagtccggca tgtttctttg cttttctgaa agctctcgag
     1021 agcccttaaa cccttacggc gcgatgggcc agtggggtta tcgcggggta accagcgggc
     1081 tgacttcgaa agcccacgaa gtcccgcgat tcgccctggg aacgccggac tttagtccgg
     1141 catgtctttt ctttttccgg gagaaccctg tcccttgcca gtttttcctc tgtgttctcc
     1201 gtgctctctg tgtgagcttt ttccaagaca tgccaacttt aaagttggcg ttcccagaga
     1261 gacgagccgg ctggaagtcg gccccacaaa aagaaggggc gcttacgccg cgatgctcgt
     1321 gtgttatcgc ggggtaacca gcgggctgac ttcgaaagcc cacgaagtcc cgcgattcgc
     1381 cctgggaacg ccgcatttta gtgtggtatg ggttcattgt tatctccgtg ccctcagttc
     1441 cctccttgcg aaccctgttt tctgggaacg ccggacttta gtccggcatg tttctttgct
     1501 tttctgaaag ctctcgagag cccttaaacc cttacggcgc gatgggccag tggggttatc
     1561 gcggggtaag tggtttttac gaccccgcga tccgcccttt gccttttaac cgcgatgggc
     1621 gcagaaaaaa tcgtatcgcg ccctcgcgaa aagaaggggc gcttacgccg cgatgctcgt
     1681 atgtgttatc gcggggtaac cagcgggctg acttcgaaag cccacgaagt ctcgcgatcc
     1741 tgatgaagcc ctacgatcag cacgtataaa agcgtatcgc gccgtcgcga aaagaagggg
     1801 cgcttacgcc gcgatgctcg tgtgttatcg cggggtaacc agcgggctga cttcgaaagc
     1861 ccacgaagtc tcgcgatcct gatgaagccc tacgatcagc acgtataaaa gcgtatcgcg
     1921 ccgtcgcaaa aagaaggggc gcttacgccg cgatgactac gtgggcgaaa aaccgcgcga
     1981 ttgattctgg cctcgtttct tggttacacc tcataacttg acacccacac gggggggatc
     2041 cccgatgatt ttcttatgct ataatcaaga gggggatgac gatgaaaaaa aagggcttat
     2101 ggatagtcgc gatcggcgcc gtcgttctgt tttgctccgg ctgcttcata caggacacga
     2161 tttcgtatat acaaagcgtt gaggatatcg tcaacacggt gtacgtgatc ggaatcgtgg
     2221 ggtttgttgc cggatggttg ctcttccaca aagagtagtc gcgttgtctc gagatttcgt
     2281 acttctattt ttatcacggg ctccgacgcg agcgcgccct cttgccacat ttcgttttaa
     2341 gtcgttatag gacgacagtc tttgcttttt tgcttgaaag gagccgaaaa tgtcaaaacg
     2401 cgttttcctc ttgtctttct taatcctaat cacggtctcc gtgggacttt ccttttcagt
     2461 gtgggtttcc tgggaagggg aagactggta tcggatgatc gcgaaagcct acaccgaggc
     2521 gactcagaca ccggtcgaga tcgtcccgat accggcaccc gaccaaaagc ttctagtggc
     2581 gttaaaaacc ccggacacct tgcccgacct ttgcttgatt aaaagcgacg attttccgct
     2641 ggctgcgacg atcgcccttc ctatcgattt gccgcggctg ccccagtatg cggatcgttt
     2701 cgcacagatc gggatacaga cctacacttc gggcggcgcg ttacgcgcgg ttccttttta
     2761 cgccgatacc caagtgctct ttttacaaac ggccgtcttc gaaaagtatc ggattcctct
     2821 tcctgaaccc gattggaaca tcggcgattt cgaagcgctc ctggcccatt tacgcgccga
     2881 aggcgtgatg ccgatcggct ggggattcaa ttccccgtac atctttcagg ggttgcagga
     2941 aggctttggc cagtctttcc taccggacgg taccatccgc gtcatcacag aagaaaacac
     3001 ccgcttgctc aagagaatgc gctcctgggt gacgaacggt tggttcaaag actatccgca
     3061 acggccggga ttggtcaaag atttctccgc cggaaaggtg gcgatgatgc cacagggcag
     3121 ctatctcatt cccgatttgg atgagatggg ggtcccttat gccctcgctc cactacccga
     3181 accatggcgg tcggtcatcg acacgaaggc ctgggtgatc ttcaaaaacg agacgctcgt
     3241 ctatcccttt ctttcctttc tcttcgaacg aatggaaggc ctgtgcgcga cgtatgcgaa
     3301 acaaagcttg atcgaaggga ttcctttctc cggaaacgcc gggggcgtat tggagagagc
     3361 gttagaaaac ggcctcgtcc aaccatccac acgcgcgtat tcggaagggt attggaccgc
     3421 gatgaaagcg tgtttggagt tgtatttacg cggagaaggg gaactgcaag aactcttgtc
     3481 caccgctcaa gcctacatcg atcagtttca atgacctttt ttctcccatt ttttctgctc
     3541 atcgccggaa tgaccctctt tccggccgta ttttcggcga tcgccggcca agcgcagata
     3601 cgattcgacc tcttttggac cttgttccaa gacggggctt tccgaatttc cgctcaagta
     3661 tccgctctgt acgcgttcat cgccacccta tcggtcttct tctgcggtac aacgctcgct
     3721 tacctcctca tccgattgcg gaggttttgg agaaaagcgg tggctctgct tctgttcgtg
     3781 atatgggcgc ttccttcttt cgtttccctg cccatatttc gagcgcttct gctcaatacg
     3841 atcgaaaacc ccatcgcttc ggaaatccaa gccttctgtt ccatcctggt cgggcgtttt
     3901 tgggtggacc ttcctgtcgt ggccctgatc gcactggcgc tattcgaaga gctccctcct
     3961 tcgtatgagg aaagtatgcg gctcgaaggc gccggaaccg tcgaccgctt tttttccctc
     4021 ttcttccaga tcgctcaaaa aagcttggtc ggctatctct gcgtttcctt tctggacctt
     4081 atgcgcgacg tcaatgtccc gatgatgatc accgaaggcc gccctttatc ctacgcgggc
     4141 ttcacgcctt atgggattgc gggtgcgacg acgacgctgg gattcttttt gaaaaacaac
     4201 ctgctagact ttgctgcaac ggactcttgg accttcttat ggtcgcaaaa cgctttggtc
     4261 accgtcaccc tcttggccgg cttctggttc ctgaaccgga tgttcaagcg tccggaaaag
     4321 accttcggga tttggctcgg gttggagatc gtgtggagtt tttccctcgg atccttgttg
     4381 tttttacccg cgtttttctt cagagactgg catcgtcgag gtcatctacg cgcttgcgcg
     4441 atcatcgcct tgggggcttc tttgatcctt cggcagctct ctccgtccac cgtcatcctc
     4501 tgtttttatc tattgtttcg gctttttccg ttccaaaggg ccttcgaccg actcctcttt
     4561 tttttcaatg tcgcgttggc cgttctatgg atagcgatga gcgggggcgc tttaggcttc
     4621 ctggtttggc tttctttttc ccgataccgg tatctccccc cctttccagg gatcgcggac
     4681 gccttcaagg gtctcagttg gaacaactac gtctatctct tcgactcggg atttctcgtt
     4741 aatctgaaga acagcctttg gctgggtttg ggaagcgggt tgttgggggt gctcgttttc
     4801 tcgatggccg catacgcggc attaaagaac tcgaggtatc acaccttcgc gaaggggtta
     4861 atcggcttga ctctggtgat ggcgggaatg aacaccttcg ttcctctcgt gttgacgctg
     4921 gacgcgttag gcggcttgaa tcggtttcta ccgatcgtgt tggcgatttt gaatcagagc
     4981 gctcccatcg cgtttatgac cgcctatgct gcctttcggc aaacggatcc gacctatgcc
     5041 gagttgggac aaatcgacgg ggcctcgcgt tggcagctct tttggaagat cgtgttcccg
     5101 ctggcgtttc ccacagtagc agtcttgttt ctgtatatat ttatccgagg gtggtcgagt
     5161 ttcatcgcgc cgctgttgct gatctcgtca ccggaaaagt atcccgtctc cttgcgtctc
     5221 tacgattggg cgggcaatcc cggcttacac cacacccgtt gggggctttt cgccgccggg
     5281 gcgctcctga cggtgacgat gatggcggtg tgctccatcc cgttgcctgc gttatggcgc
     5341 aaaaggtggt cgtcgggttg acgacagacg accaccctta caggccgaag ggtctatctt
     5401 aacaccccta gctctttacc gacctcttcg aaggcgcgga tgccgtaatc gaggtcttct
     5461 ttactgtggg ccgcgctcat aatgacccgt atacgcgctt tccctttggg aaccgtcggg
     5521 aatccgagcg attgcgcaaa gacacccttt tcaaagaggg ttcggctgaa ttggctggct
     5581 aaaggcgcct cgtagagcat caccggcgtg atcggggtct gcgtgtggcc ggtatcgaat
     5641 ccgaggcgcg tcatattcga ccggaaatat tcggcgttct cccaaagctt tttcaccaac
     5701 tcgtcgcttg actccaggat tttgaccgac tcgattgcgg ccgccgtatc cgctgggctc
     5761 agagagctgc tgaacaaaaa gggacgcgcc ttctgtttca agtaatcgat cagcggtttt
     5821 ttcccggcga cgaacccgcc gatcactccg aaggccttcg aaagcgtgcc gacttcgatg
     5881 tccacctctc cgtggagatg gtaatggtcg acgatgccgc gcccgtgtga gcccaacacc
     5941 ccctcgccat gcgcgtcgtc aaccaacacg atcgcttcga actctttcgc cagagccacg
     6001 atctccggta agggcgcgat atcgccatcc atactgaaaa caccgtcggt gatgatgatc
     6061 tttcgcctag caccttcgag ctgggccttt cgcaactgtt cgcgcaagtc aaccatatcc
     6121 ttatgcttcc aaacgaagcg catcgctttc gtcagacgcg tcccatcgat aatgctggcg
     6181 tgattcaatt catccgacaa cagggcgtct tgggcgccga ccaaaggagc cagcacggct
     6241 tgattggctg taaagccgga ttggagcata atgacggctt ctaccttctt aaacgcggcc
     6301 agccgctctt cgagctggtt gtgcaaagac atcgtccccg cgatcgtccg aaccgccccc
     6361 ggaccgacgc cccagttttg gatcgcttcg attgccgcct gtttcatcgc cggatgattg
     6421 gctaacccca aatagttgtt cgaagacagg ttgagcactt ttttcgactg tatcgtcaac
     6481 caggcgccct gtggcccctc caacgtccga atccatgtgt ataacccttg ctctttcgcc
     6541 tcttccatct cattcgtgag ttccatccaa tcctgcatga ttccaactcc tttctacttt
     6601 tttcccccgg accacggcca tccttctttt gttttcgtcc ccaaacccta tgaactcgcc
     6661 ggattggaac atacacagct ttttcgttgt aatgcgattc agcgaagact tgatcatctt
     6721 cccgtaaaac tgtatcgagg atggagcgtc cgcggtctca caatacggtt caagactatc
     6781 tcgaacggag tagtacaaag tttttttgga aatccaaaac tccagctcct cgggataacg
     6841 ggataggttt aggatgccgt tatccgaaag ccgaagcgcc ttagaaaaaa gctttcgggc
     6901 ctttccggag tccacccgcg ccaccggttc catccgctcg tagacgtgca acagtcggaa
     6961 gaggtcggtc tcgtctcttt cgtccagcgc tttcatacac aaagccgtca cggccccagc
     7021 cgtttggaaa taaatctcct cgctttctag agaaacgccg atcttgagtt cattcacatc
     7081 gatgagcgaa cacgccttca caaaatggcg gatgctggca tacgggattt gggctttcaa
     7141 aaagctgccg tcttcgaagc gacgaatcgg aatctcgcta ttgaagagcg cgaagctgtc
     7201 ctgctccacg ctgaccaccg caaactgccc gtcgtaatcg accaaaaaaa cccgatcgag
     7261 cataggaaga gaaatcgtcg cgaagtctat gccgttcata aacgccttga aaggcaccgt
     7321 acacaacgaa tccggaaagg acaagtcttc tatccgcctg tcctggccct gccttaatga
     7381 caatacttcg ttatcgcaac gaaagatcgc cgattcttcg cctagaagaa cgttaacgtg
     7441 cgtatccacc gaccgttctt gcaaaaagag cttcaacggg cgtatcggaa attggtaggc
     7501 gcgctcgaaa gccggatact cccccttcgc ctggaagacc gttctcaaag aaccatccgt
     7561 gacgaaaatc cgcagcccct gatggtgaaa gagccaaaaa aacccgtatt tcttctcgga
     7621 gcccgcgatc atctcgcaat agctcacgat cttcaatagc cgctcttttt ctatactgaa
     7681 agcggcgaga ggcaacatcg cgccacttga acgaaccacg ggagtcggac tcggccagct
     7741 catacgacat ccccacgcga aaaccgtgtt aaagtcgccc aacagccggc gtcccgacac
     7801 aggctttcat aacgcgccaa gaccgacggg tcagtatcgg ccttaaacac gacgtaatga
     7861 gcggatccag aaccgctcat ccgtttgagc aagatcgccg ggtccctatg catcccgtct
     7921 tcacccagac gagagaaaaa actgtgaaaa acacggtatt ccggataata cgtcaaagcc
     7981 acctcttcaa agtcgttgcg cgaaatcacc ggcaactgtt gcgccaaggc catatagaca
     8041 tcctgagcgg tctgttggtc cgacctatcc tccctgagcg gatgattcgg gctccgtgag
     8101 accatttgat cgtactgctc gtacattttc gcggtactca tcgcgtacgg cggttggacg
     8161 agcaacaccg gcaaggcggg caacggcggt aacggggaca aggcgctccc gttttcttca
     8221 cctatcgcgg taccgcatag gagaaaaaac ggaacatcgg cgccaagccg tttcgctacg
     8281 ctccattcgg tatgcgggtc caaaccgaac cgtcgacaca aaaaccggag caacaccgcc
     8341 gcatcggcgc tgccaccgcc gagtcccgat tgcgttggga tcgttttctt taggtggatc
     8401 ttccaatgcc cgcacgtatg accggcggcg ttttctaaca cttcgaaggc tttgtacagc
     8461 aaattgcgcc catcccaacg cagttccggt tgaaaaacga tgctaggatc gtgctccaca
     8521 gagaaggagg tgggactttc cgtctcctcg agtgtcagcc tatccgagag agcgaccgtc
     8581 tggaacagcg tctgtatcgg gtggtatccg tcttgcccgc gagagaagac ccgtaaaaaa
     8641 agattgatct tcgcccgcgc ctctaatacc gtgacctgtc tcttcgcgga ggttattgta
     8701 aggcctctat ccgttttttt gcctccgaaa ccctcgaaga cgcggggtac ttcgcaacca
     8761 ggtcttggta tattttcagg gccttgtccc tctcgccgga cttctcgtag cataagccga
     8821 tgtagtaata ggcggtgtcg tttttggaaa aatccggata ggaggtgatc agcttaaaaa
     8881 actgattctt cgcatttgtg taatcgttct tgttatagta gtacagggca taatcctgta
     8941 tcgcccaggg tgttttcggg gattgcgggt actgatcgat cagctgctcg aaggccgcgc
     9001 gcatctcccc ttcgttcccc tgagaggcgt agtatttccc gcgtatatac aggtaccctc
     9061 ccttagatcc cgcggacaac cgatcgaagc ttgcgtccct caacgcttcg aaagcggaaa
     9121 tgaggtcgcc ttttttcaaa taggcttcgg ctctcagata ccttaactcc gcgctgagag
     9181 cagagtccga cgtcacttga agggccgcat cgatctgccg ggcgatttcc agcgggtcct
     9241 gattcgtatc caacatcacc gtatactttt tcagagtcac cgtcttatag tactttttcg
     9301 aaacgtcgtt tctcaaaatc caatcgtact cgttcaacgc cgaagcgggc agattccgcg
     9361 cgatataggt ttcggccata tagaacgccg cgtccagcgc cgcgctgagg tctttcgtga
     9421 tctctgtttt caaggtttca aactgcttct gcgcggtcgc gtagtctttg gcattcagcg
     9481 cgacgattcc gaggttcagg atcgcttggc ttctttccgc ttcggaggcg ttcttcaagg
     9541 cattttcgta cgcgatcttc gccttttgat aatcgccggt ctggaggtag agatcgcctt
     9601 ggaaggtata cccttggaag acagcggctt gatttcccga gcggataacc cggtcgacgt
     9661 agtcttgggc ccgcttcata tctcccgtct tctggtagag ttgggcgagc gtgtagttcg
     9721 cgtcctgata aaaaagaccg gacgacaaga cacgctgtaa cagaacctcc gcttccgagt
     9781 accgccccaa ggagagatac actttcgcgc tctccaggtc caaggccgtc ccttgcgaag
     9841 gaaaggtggc cttcatctgg ttcagatagg acaccatctt cgccgaatcc cccgtgttac
     9901 gtaaaacatt gatcgcctgt ttcgccactt cgaaggtttc gatcggatac tccgcggcgc
     9961 ctacgctgat cgctttgttc cagttggcca aagcggtctc gtaattaccc gacttgtagg
    10021 cgagccaacc catcgcattg taggcgtcga ccaaatcacg ggtgcttttg gcgttttcga
    10081 tgatgtaatc gatatagcgc gcggcgttgg catattcgcc cgtgcgatac aacagttcca
    10141 cgtgcagcat gaagatctca tcgcgatagc gggttgtgga atagttgcga accaagttct
    10201 cggagtaact caacgcctcc cgataattct gaaggttcat catggcgcgg atcgtctggt
    10261 atatgctggc ggccatcagc tccgggctct gcccgaactg tttcgcccgc tcgaaagcca
    10321 tgatcgccga agggtagttc ccttgctcaa agaagatcaa tcctttgtag tagctggcga
    10381 atcctatggc gctcgggtcg gttaccgcgt cgaaactgga ggaagcggcg aggtaatcgc
    10441 ccttcgcttt ctggatcatc cctttcagca aataatagtc ctgttggtat ccggaaaaac
    10501 tgaccaattg ctctaaaagg atcaacgcct gattcaacag agagtcgcgc tggctttggt
    10561 gggtctccat cgccatatga taggtcgcgt aacacttcga atagaccgcg taaggatagt
    10621 cttgatcatc tttaccggtg tattgcagcg ctttctcgta agcttctatg gccgtactgt
    10681 aatagccttt gtagaagtag gcgtctcccg tgagcaagtg agctttcgaa aggacgtaag
    10741 ggtcggaacc tcgatccaat atgatcttct gaagatcaat gatcgcggca tccaacaaag
    10801 aatagtcgtt gagatcgtaa gccagtagaa tcttggattg gacgcttttc agctcgaaca
    10861 aatcttgaga aaaggccgtc agcgtcaggc aaatgcataa gacaaccagc gtgagcgtcg
    10921 ttttcatcaa acccccccat ttatccgtca ttaaaataag cctttctgaa gtcttccagt
    10981 tcattttcat cgatctccct gatctgcccg ggaatttcta ttccttcgag ggtaaagggc
    11041 ccgatttgat cccgccgcag gtacaccacc cgagacccga ccgattcaaa gagacggcgt
    11101 acctgccgct tcttcccctc gtgtatcgtg atccgccagc acggtcggcc tgtaaagcct
    11161 ctcttttcca gacgcgtttc ggacagtttt gctttcgcgg tcttcacgcc gtcgatcaca
    11221 accccggatt ccaagaggag tatttgttcg tgaccgatct cactctgcaa gaccgccagg
    11281 tagaccttcg ggatctcgtg agagggatgc gtgatccggt acgtgaagtc cccgtcatcc
    11341 gtgagaatga gcagcccctc ggtctcctta tccaaccgac ccgtcggatg cagcggcccc
    11401 gaaagaccgt gaacgcgtct gacgtaatct atgaccgtag gccgtccttg agggtccgat
    11461 accgtcgtga tgagtccgct cggtttatgg aacaggtaat agcgatgacc caggggcaac
    11521 gcgcaaaccg ccccgtcgac cgtgatgcga tccttctccg ggtccacggg cgcccaaaaa
    11581 tcgcgaacga cgactccgtt caccctcacc cgtcctccct cgatgatcgc attgaccttt
    11641 cgccgcgcgg ccagtccgca ccgatggaga taatactgca gcgtcatctt aaaagggctc
    11701 gatgagacaa accgacagcc cgattttccg agcggatatc tcgatttcca cggtgagatg
    11761 gtccctgaac cgatgacgat cgctttcgat gaaaaatcgc gtcttaaact cgacggtgcc
    11821 caacaaccag tccattgtgt tcaaagtatt cgggcttcga atgatcggaa acccgatacc
    11881 caactgaaac atccacgggt gaagatagta ttggccgtat aaaccagcgc ccaaccagta
    11941 tccgctgttt tgccacactt cccaattgga taggtcagag gaagtttctg aagggtcctt
    12001 ataggtgtcg taaaaattcc caaactcgaa ggtgccgaac aagccgaagc ggtacccttc
    12061 tctcgtttcc cgccggccta acaacggaat cataacatcc acatcgaaat tgaggtagtt
    12121 cgtaggcgtg gcctctcgct ctcccgattc gctgaaagag aactgaggat gataggtgac
    12181 atcgagggtg agcatatcat tgaactggtt gaataacccc aaatccaggt tccagccgtg
    12241 gttggcgaaa ctgaacggtg cgaagcgata gcccgttccg atctcgaaca acataccaaa
    12301 gaccgatagc ccgaagagcg ataagagaag aaacgcgcaa agtcgaggtc tcatacgaac
    12361 ccttctcgtc cgggtaggta ttgggagccg atcttcgtaa ccagcccctt aagcgacaat
    12421 cctgtcaaga tgcggttcag ggtcgacgct tgatatcggg tcctctcggc taggctatcg
    12481 aactggtcac agccctctac gatgagatca tagacggctc tttcctcgga agccaaggag
    12541 gcgctatcgg cgccagctgc tggactcgcc agggtatcgg ggaccaacca ggggtattcc
    12601 tcgacgatat ccagcgcgtc cgtcaccaac ttggctcctt tcttgatcaa tccgttggtg
    12661 ccctctgaca aagcgctctg tatcgacccg gggatggcga agacatcccg gttctgagag
    12721 agtgcgtgct ggaccgttat caacgaaccg ctcgtcttgg aggcttcgat gaccaaaacc
    12781 ccttggctta accccgagat gatccgattt ctttcaggga aaaagtagac ctccggtttt
    12841 tgccaaggca agtattcgct gaccagacag ccctgttctt cgaccttcct gaaggcgacc
    12901 gtgttggatg cggggtagat gtggtccaca ccgaagccca atacgccgat cgtgtgggtg
    12961 cctttctcga tcgccgtcaa atgggccatc acgtctattc cgtatgcgag tccgctcacg
    13021 atggtcaccc gacgttcggc taactgaccg atgatctttt gaactacgta cttcccgtat
    13081 gatgacggtt ttcgtgagcc gacacacgcg atcttcagtt ccgggctgga tagccactcc
    13141 gcttgccctt tgatgaacaa gacctggggc atcggatcga tctccctgag caaaggggga
    13201 taaccctctt cttcgggaaa gaagatcgtg aggtctttgc gccctgaaac tttccggcaa
    13261 cgatcggcga tatcggcgtt ccacatctgc tcgactcttc gtttctttct ttcgagcata
    13321 ggggcggttt cggatttcaa gaactccgaa atcgaagaaa aggcttcgac cgtatcgatt
    13381 tccgcggggg tgaagatctt cgaaccccgg agggccaatc gttctatccg ttccacgggt
    13441 tatccccttt acaaaatgta ggatgagaga tcctggtcgg agacgatcgg gctcataacc
    13501 gaacggacgt agtctccgtc tatcatccta taggtgttat cggtatccgg cgcgtcatat
    13561 gatatctctt ccaaaacctt ttctagaacc gtgtatagcc ttctcgcgcc gatgttttcc
    13621 aaccgttcgt tgagctcgaa agaaaggtgc gcgatctcgt gtattcccgt atcgtcgaaa
    13681 acgagggtca cgccgtctgt ttcgagcaaa gctttgtact gtttcaccaa ggaacattcc
    13741 ggttccagca agatcctctc gaaatcccgc tcggtcaggg ggtccaattc gactcggatg
    13801 ggaaaccgtc cctgcatttc gggaatgaga tcggacggtt tcgagatgtg aaacgctccg
    13861 gcagcgataa acaagatatg atccgtacgc acgataccgt acttggtcat cacggtcgtg
    13921 ccgtctatta tcgggagcaa gtctcgctga acgccctctc gggacacgtc cggccccgaa
    13981 accgacttcg aggaggaagc gattttgtcc atctcgtcga taaagatgat cccgtcattt
    14041 tgggccaact ccaaggcgtc ggaggtgatt ttatcggtgt caatcaactt ctccgattct
    14101 ataggcatca agatttttcg ggcttgggcg acggtcatcc gcttttttat ttttttaggt
    14161 ggcatcagat tgctcaaaac gccctgcaaa tcgatttcca gatcgctcat atcccctccg
    14221 agacccatca cgccgagggg gggttgctga gccttttcga tctcgatatc gatgaactgg
    14281 tcatccaact cgccgtgacg atagcgttcg gcgaggtcca tcgggtgtgg gggcggcgta
    14341 tcctcctcga aggtttccgc tttctgactc gtctgtctgt cgcggtctcc gaaggactcc
    14401 atcgtaattt tcgaatccaa aaaacggctg aagaagtccc cgagtttctt ttccggttgc
    14461 ttgacggacg ttttcgtgat cgcgttgagg atgatcttct ccacctcttt ttgcgccaga
    14521 gccaccacct tctgcatctc ttcctttttc agaaagttga tcgcagattc caccagctct
    14581 cgaaccatcg agtctacgct ccgccccacg taacccactt cggtaaactt cgtggcctcc
    14641 accttcacaa acggagaccc ggaaagaaag gccagtctcc tggcgatctc ggtttttccg
    14701 acacccgtgg ggcctatcat gagtatattt ttcggaatga cctctttcct caaaggatcg
    14761 agtagttttt gacggcgaat ccgattgcgc aatgcgatcg ccacggcctt tttggcagcc
    14821 gtttgaccca cgatatac
//
LOCUS       DAOPXN010000049        14789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4134_length_14789_cov_5.352179, whole genome shotgun sequence.
ACCESSION   DAOPXN010000049 DAOPXN010000000
VERSION     DAOPXN010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 14789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14789
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4134_length_14789_cov_5.352179"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1730)
                     /locus_tag="PKM49_04140"
     CDS             complement(72..1730)
                     /locus_tag="PKM49_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82037.1"
                     /translation="MPEKIIYALPEEIGNTELFVGRKKEFDYYLGDWYYYLVNNMAQS
                     QAIVARRKKGKTAFLQRLFNILWSCSESKVIPFYYAIQEEQITRASFAKEFFATFASH
                     YLSFLTRNEEWVRTPFDYVEMKEYITQYPDLYKKSKSIDAYEKTGNWDLMWKVASRIP
                     FDIAVSTGVKVVQIIDEFQNINAYILDERGNTIDTMSGAYLILAEKREAPLIISGSEV
                     HGLLEIVRRLTARFKERTLGNLPEDEAKEAIRRYAKASRTKINEQAEEKIWNLTQGDP
                     LYIRALMQSEHNDTKDYTNEDNIIATYTQEITQGEIYGTWTEYIAKIFVEVNKRNAKR
                     ILLYLFQAGEERTRAQIIKDLKLDITDSELETKLHKLIKADLISRGETYFDYKIGKDK
                     TYELVFRHLFQKEIDYFVPDIKKELRQEMGRTSYEKGKFREYLVRERIKKPFNLKDLA
                     ENGIDLTIKPKTILERETVKVGLRDREIDLIVKGNVELWIDVKDTKGKYGKREAGRWI
                     EIKQAIGEKSPKTLFATYSQNRYTAAAKELLVSNGVYVLKGEEG"
     gene            2183..2932
                     /locus_tag="PKM49_04145"
     CDS             2183..2932
                     /locus_tag="PKM49_04145"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY82038.1"
                     /translation="MRLPDYLKQTGAITDERLYRAFCAIDRASFLPEDRQEEAHRDWV
                     IPTYQVRGRSFSTNSQPSLIAAMIEMLRLTGSERVLEIGTGTGYCTALLASMLDLGAV
                     YSVDILDHLVEEARKNLSRYGITNARLKVGDGFFGWEEEAPFDAILATVAIGDIPGPL
                     FNQLKVGGLFVCPLYIHQGRTPVYLLERLSLLEVIGSFRIDAVFITMADHIGPEENRL
                     QKPKTRIRFRKETPDSPYLLSTVNGGRERWF"
     gene            2932..4086
                     /locus_tag="PKM49_04150"
     CDS             2932..4086
                     /locus_tag="PKM49_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82039.1"
                     /translation="MGIFIDSVVELLMYANLAMFNILGQYFYMRGFSPFEVGILVAMI
                     PAASFVSNPFWFRMKEKLPRTRLIAILTIGTVVGFWSLFFVRPFLLNLVLMAFTAFFS
                     GAVVPIGESYVMESLIRKQKKLDLPRMFGTIGYSMGSLMIGFLLKIDVVFLFAFSTLF
                     LVLVSFLIRRLDQIALVSEGRSAASLPRTGSWNAFLWYLVLASASILLISFNGAFLPQ
                     LIGQRGYDPALTGICFAVLSFSEVPFLLFAKKILRKMGTLLLLATGVLCISLRMFLTP
                     FTNSELALVLVQAMHGWSYIVIYYALFDYIHYELPIAYASKAQAVFWIGIQGLSFFLG
                     SSLGGLLVEGIGLDRTYLLLSIGLFCVAAPLTVVAVVKRFKGSGMKRKTA"
     gene            complement(4670..5122)
                     /locus_tag="PKM49_04155"
     CDS             complement(4670..5122)
                     /locus_tag="PKM49_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82040.1"
                     /translation="MRKILFILLTVFSVTTLVCGPMIATTDDGRKVILYENRTWDWAQ
                     SGSPSLRYNLTGQWDFSWTLGIFDETMSAQITHGQTSITLTWKDTFGTTNVTGRIVSS
                     NELTFNGKSFYNGEITFKATVIDNNTLKGTVSSGGAFPKTGNFTAKKR"
     gene            complement(5134..5883)
                     /locus_tag="PKM49_04160"
     CDS             complement(5134..5883)
                     /locus_tag="PKM49_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HNY82041.1"
                     /translation="MEIDLIPFYYTGCETLYEPSDVCFLIDVYRASSMVVALAQRGAE
                     RFFLTAEVQAALCWKERHPEVLLYGERGGVAPPGFDGGNSPTELENRSLTGKTVVLTT
                     TNGTRAAAAFWKKTGRLFALSLVNFEAAVSWVERNFVIDGRGRGRVTILCAGTRNRLS
                     LEDFGVGALFWRTLKRYDEAPVSDEKTLAEFLAPRLQDESMLRATLHHVRHAMTLRSL
                     GFSNDLDWILSPKNRFSVLPELHLLNVESSD"
     gene            complement(5888..8047)
                     /gene="rnr"
                     /locus_tag="PKM49_04165"
     CDS             complement(5888..8047)
                     /gene="rnr"
                     /locus_tag="PKM49_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNY82042.1"
                     /translation="MQKDKSAVLEHLRTQAVPLLLRELYRVFGANTKSEKAEFRAFIK
                     RLEAEGAIEKNETNRYRIPEDEEASESGEKASSHGIAGLLDFSRNGHIAFCAASDGIE
                     YVIFPEDSKYAIHHDLVQIKPSGFYKDWRRAQVVKILQRNQKDVVGILAREGQNYFII
                     PDDKRMGLRYYLTGAEKPDRLGSELGSKVIAHIVSYPSKKKTPVAKITHYLGSIEDPR
                     VHLPTVIVKHGLPFPEDFPQGVMEEAKRIPDRIFPKDTKNRKDYRNQIVFTIDKETAK
                     DFDDAVGIEKLDNGHYLLGVHIADVSAYVPMDSELDREAYRRGTSTYLLDTVVPMLPL
                     ALSNGICSLIENRSRLTLSLEMEVDSNGRLVRYECHEGVIKSKKRLTYTAVNDFLNGN
                     TPSEGAKLFAKNPEIGQALILMDELAKILRENRKSRGAIIEIESGEVEIPLDEVGNAL
                     DILVKKRDRAESFIEEFMLLANETVASIFADKPEAFIYRVHERPDPETLIQLKRYLLA
                     LGVQKKLPKEMRSSDIQAYLETLQDHPLKSSIQRLVIRSMKRALYSEENIGHYGLASE
                     NYTHFTAPIRRYPDLVVHRMLKGWMEHRPAPYNSEQLTKIARQSSVRERVSSEAEWDL
                     VALKKVNYVSRNLAELYQVVVTNVARFGLFVEVPEKLIPGLIPISTLDDYFVFDEERN
                     ILVGERTRKVYKIGDILEVRVKGIDHVRGEIDFELLK"
     gene            complement(8088..8975)
                     /locus_tag="PKM49_04170"
     CDS             complement(8088..8975)
                     /locus_tag="PKM49_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY82043.1"
                     /translation="MEIIVFSKAMYSTWLYYAPDRIMFDCGEGVSTFLTNKIYAVQRI
                     FLSHGHADHISGLWGFLNTRNSAMGEREKPLEIYYHSSSSRIRKYLEFIVESSNRLRY
                     DILVREIDEGFTLPLFPKEEGAPTRSALDRKAKKSRYLEAFSTKHMETECTLGFHIKE
                     TRKRLKEPYRSMSQSDIRKAIQSKGSEEISEYYDQNLFTYSGDGLVMPRSRMENTDTL
                     FHECTFLDIDERKGENHTSLEELVEPVLAAKPKELYLYHISSRYTDSLRERIEKFERK
                     MAENNVKVNVVPPGKICYF"
     gene            complement(8997..9446)
                     /gene="rplI"
                     /locus_tag="PKM49_04175"
     CDS             complement(8997..9446)
                     /gene="rplI"
                     /locus_tag="PKM49_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNY82044.1"
                     /translation="MKVILLQDVPKIGKKGEVKTVADGYARNFLIPKGLVKEATEGTL
                     KHSQSIAQIRKDHQDKMQQKSQEKLDRLTKEFFTIYAKAGEGKKLYGSITNADIAQEL
                     EKVLEEPVDKRCISLSAPIKEIGVYEVGVKFSGGVRGVLKIKVEKTP"
     gene            complement(9567..10736)
                     /locus_tag="PKM49_04180"
     CDS             complement(9567..10736)
                     /locus_tag="PKM49_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNY82045.1"
                     /translation="MNTSAQREKTATCVVIGSTGSIGTQTLQVIDRLSARGYGMRVVG
                     LAARSHPSFIQQLKTYRPAGYCFERPLTGIPETARRYGAVEEMLDALHPDMTMIASSG
                     GISLSYALKALETSKRLCLANKESVVLGGRLIPERAKALGKELIPVDSEHSGLFQLLL
                     GTDRKEIRKVYITASGGALRDWDESRKENASVEQVLRHPNWQMGAKITVDSATLMNKA
                     LEMIEAKFLFDLPKETIAAAFCHNSFVHAVLAYWDGHFKIHAGPPDMRIPIAYSLTYP
                     ERVSVLEENGTAVEDPRSLFEPMILKPADTAVHPPLRLAQAVIDAPSSLRIALNAANE
                     AAVLLFLQRKVTFGELTRLVERTVLSQEAYEVTELSQILELHEGIKRRVELDYSC"
     gene            complement(10789..11580)
                     /locus_tag="PKM49_04185"
     CDS             complement(10789..11580)
                     /locus_tag="PKM49_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNY82046.1"
                     /translation="MTRFSYSKLSAFDQCPLKFRYQYVQQVPSKTSPALFLGQIVHDT
                     IAAYEKALQRKSSGQIVAGVMFSTENEDLEWLHQTFRQKWMAERNKCRQQGLLEMSRS
                     EEAIYGIRGLRMLENFYKSPFASCPYETERFLDVPLGEDLIFVGRIDRIEKRQTGFRI
                     VDFKTGKFSERFIDFLQLHTYSWLAYQYGLKVDSTLFYFLEDDELVERPFSHEQNQRT
                     EAALKEKCQTVLLAAQNNAFPKKKGPLCAYCDYQDLCDSESAPPL"
     gene            complement(11577..12572)
                     /gene="fmt"
                     /locus_tag="PKM49_04190"
     CDS             complement(11577..12572)
                     /gene="fmt"
                     /locus_tag="PKM49_04190"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNY82047.1"
                     /translation="MSGFFNPFLRRVVFLGTPEFAANYLEPLLQEGVPIVAVFTGEDA
                     VSARGNKRVAPPVKQTALQYGVPVFQPRQINRGEGFESLARYQPDLILLIAYGKLLRE
                     PALELPRYGAYNLHPSLLPRYRGAAPIQRCLLNGDNETGVCLMKMSLALDEGPVARSV
                     TMPVSADQTYSGLVHQMRETALPMLLDFLRGFDPATVHLTEQDPAQELSYAAKVEKEE
                     WEVRWADSSQAVHNRIRAFDADPLARTAYKGVTVKLAGSRLYDGPEEKPPASASSKPT
                     PGTILDITKQGARIACGMGSVIVGRIQFPGKSLISTYQATQGRLLSLGERFEGPI"
     gene            complement(12572..13147)
                     /gene="def"
                     /locus_tag="PKM49_04195"
     CDS             complement(12572..13147)
                     /gene="def"
                     /locus_tag="PKM49_04195"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNY82048.1"
                     /translation="MTYHVRYYGDPILRSKSKRVESFDEDFRRFATRLVELMYEYDGV
                     GLAAVQIGVPKRVFAIDDGSGGGWKVIVNPEITWRSKESVINEEGCLSLPEIYEDVER
                     PQSIAVRYQNLEGETVEERLEGYPAIVFQHEADHLNGVLFIDHISVAKRRLLHRTLLD
                     IQRKAIPRMAADFVEPRPASSESNPKAKETL"
     gene            complement(13165..13923)
                     /gene="surE"
                     /locus_tag="PKM49_04200"
     CDS             complement(13165..13923)
                     /gene="surE"
                     /locus_tag="PKM49_04200"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNY82049.1"
                     /translation="MNILITNDDGITAPGLKVLKDYLTNAGHAALVCAPDTERSAIGH
                     AITLRMPVWAKSVSLNGRFFGYASSGTPADCVKLGILQLAPFKIDLVVSGINHGANLG
                     TDVPYSGTVSGALEGAIMGYPSIAISDVSHKESTYETAAAFLVDFLKDFDPKSIPPFG
                     ALNINVPTVPLSELKGFKITKQSNRRYNDFYEKRQDPYGNDYFWLMGDVIEEQPSFES
                     DYYAVMNKYVSITPLSVFMNDDKAFEQLRRQYPG"
     gene            complement(14503..>14789)
                     /locus_tag="PKM49_04205"
     CDS             complement(14503..>14789)
                     /locus_tag="PKM49_04205"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82050.1"
                     /translation="MQTIAEWLKQEGMEKGMEKGMKEGMEKGLAKGIIKGKEEGREEL
                     LWKLISKKFPQIPSRYYEKLKALTIDQLDTLGLDLMEMQSEEELKRHLLM"
BASE COUNT         3074 a         4019 c         3846 g         3850 t
ORIGIN      
        1 gagcggatcg cggggtcctg ttagttaccc cgcgataacc tataaagttc ggcgttccca
       61 gggcgctcct tctacccctc ttctccctta agcacgtaca ccccattcga tacgaggagt
      121 tcttttgctg cagccgtgta cctgttttgc gagtatgtcg cgaataaagt tttcgggctt
      181 ttttcaccga ttgcctgctt gatctctatc cagcggcccg cttcgcgttt cccgtacttc
      241 cctttcgtgt ctttcacgtc gatccacaac tcgacattgc ctttcacgat cagatctatc
      301 tctcgatccc gtagtcccac tttgaccgtt tctcgctcta agatcgtttt cggcttgatg
      361 gtgaggtcga tcccgttctc cgcgaggtct ttcaggttga acggcttctt gatacgctct
      421 cgtacaaggt attctctgaa ctttcccttc tcatagctcg tccttcccat ctcttgacga
      481 agctctttct ttatgtccgg gacaaagtag tcgatctctt tttgaaagag gtgtcgaaag
      541 acgagttcgt aggttttgtc ttttccgatc ttgtaatcga agtatgtttc acctcgcgag
      601 atcagatccg ctttgatcaa cttatgaagt ttcgtttcca gttcagagtc ggttatatcg
      661 agcttgaggt ctttgattat ctgcgctcgg gtgcgctctt cgccagcttg aaataaatac
      721 agtaagatcc gctttgcatt ccttttattc acttctacga atatcttcgc gatatattcg
      781 gtccaggtgc cgtagatctc tccttgtgtg atctcttgcg tgtacgtcgc gatgatgttg
      841 tcttcgttcg tgtagtcttt tgtatcgtta tgctcgctct gcatcagcgc gcggatatac
      901 aacgggtccc cttgcgtcag attccatatc ttttcttccg cctgttcgtt gattttggtc
      961 cgcgaggcct tcgcataccg ccgaatcgct tctttggctt catcttccgg gagattccca
     1021 agtgttcgtt ctttgaaccg tgccgtgagt cttcgtacga tctccaacag tccgtggact
     1081 tcactccccg aaatgataag cggcgcctct ctcttctcgg cgaggatcag gtacgcccca
     1141 ctcatcgtat caatcgtatt cccacgttca tcaaggatat aggcgttgat gttctgaaat
     1201 tcgtcgatga tttggacgac tttaacccct gtcgacacgg cgatatcaaa cggaatccgg
     1261 cttgcgacct tccacatcaa atcccagttg cctgtttttt catatgcgtc tatcgatttg
     1321 ctttttttat agagatcagg gtattgggta atgtattctt tcatttccac gtaatcgaaa
     1381 ggggtcctaa cccattcttc gttccgagtg agaaacgaaa ggtagtgact tgcgaacgtc
     1441 gcgaagaatt cttttgcgaa gctggcgcgg gtgatctgtt cctcttgtat cgcataatag
     1501 aaaggaatca ccttgctttc cgaacagctc cacaggatat tgaacaatcg ctgcagaaac
     1561 gccgttttac ctttcttccg tctggcgacg atcgcttggc tttgggccat attattgacc
     1621 aggtagtagt accaatctcc cagatagtag tcgaactcct tctttctccc gacgaagagc
     1681 tcggtgtttc ctatttcctc cggtaaggcg tagattatct tttccggcat cttgaacatc
     1741 gctttcaccc gctttgtttc ggtttggaaa gattatagca tagagtggaa gtctgggtgc
     1801 aactagtagc gccgactccc agtcggcctg ccttgtaaaa aggtctcgca cagagatcac
     1861 agagggcacg gaggacacaa aggagagaga ttttaaagag agtcaacact tggttattgt
     1921 tttctctgtg ttctctccgt gtactctgtg cgaaccttct tttggttttg gaaagacata
     1981 aagctgatcg cgagacttcg tgggcttccg aagtcagccc gctggttacc ccacgatgaa
     2041 ccgcaaattg gcgttcctag gtgatgcgcg aagggataca gccggctgaa tgtccgtaac
     2101 accttttcca ccgaaatagc atataataca cgcgaaaagt gcgggctggg ggtccacctc
     2161 accgaacgcg ggaggaacag atatgcggtt gccagattac ttaaaacaga cgggcgcgat
     2221 cacggatgag aggctttatc gcgccttttg cgcgatagat cgggcgtcct ttcttcccga
     2281 agatcgacaa gaggaggccc atagagactg ggtgatcccg acctaccaag ttcgtggaag
     2341 aagtttcagc acgaactccc aaccgtcgct catcgccgcg atgatcgaga tgctccggct
     2401 cacggggtca gaacgtgtgc tggagatcgg aacgggtacc ggctattgta cggcgcttct
     2461 tgcctctatg ctcgacctcg gagccgtcta ctccgtcgac atcttggatc acttggtcga
     2521 ggaagcgcgg aaaaacctat cccggtatgg gatcacgaac gcacgtttga aagtcggaga
     2581 tggttttttc ggctgggagg aggaggcgcc tttcgacgcg atcttggcga cggtcgcgat
     2641 cggtgatata ccggggccgc tttttaatca gctgaaagtg gggggattgt ttgtctgccc
     2701 gctgtacatc catcaaggcc gaacaccggt ctacttgttg gaaaggctct cgcttctgga
     2761 agtcatcggg tccttccgta tcgacgcggt ctttatcacg atggccgatc acatcgggcc
     2821 cgaagaaaac cgccttcaaa aacctaaaac ccggatacgc tttcgtaaag agacccccga
     2881 ttctccttac ctgctgagca cggtaaacgg cggaagagag agatggttct gatgggcatc
     2941 tttatcgatt ctgtcgtcga attgctcatg tacgccaacc tagcgatgtt caacatcttg
     3001 gggcagtact tctatatgcg cggattctct cccttcgaag tcggtatcct cgtcgcgatg
     3061 atcccagccg cctccttcgt ttcgaacccc ttctggttcc ggatgaaaga gaagctaccc
     3121 agaactcggc tgatcgcgat tctgacaatc ggaacagtcg tcgggttctg gagcctgttc
     3181 ttcgtccgtc ccttcctatt gaatttggtt ttgatggcct ttaccgcgtt tttctcgggt
     3241 gcggtggttc cgataggcga gtcttacgtg atggagtctt taatccgtaa acaaaaaaag
     3301 ctcgacctgc cgcgtatgtt cggcacgatc ggctattcga tgggttcttt gatgatcggc
     3361 tttctcctga agatcgatgt ggtctttttg ttcgccttct cgacgctctt tctggtcctc
     3421 gtctccttcc tgattcgtcg tttggaccag atcgccctcg tgagcgaggg tagaagcgcg
     3481 gcctctcttc ccagaaccgg gagctggaat gcctttctat ggtacctggt tctcgccagc
     3541 gcctcgattc tgctgatctc tttcaacgga gcctttttgc cacaactgat cgggcaacgg
     3601 ggttacgatc ccgcgttaac cggtatctgt ttcgccgtgt tgtccttttc ggaggttccc
     3661 ttcctgctct tcgcaaaaaa aatcctgcgt aaaatgggca ccttgctgct gttggcgacc
     3721 ggcgtgctgt gtatttcgct aagaatgttt ctcacgcctt tcacgaattc ggaactcgcg
     3781 ctggttctgg ttcaggctat gcacggttgg agctacatcg tcatttacta tgccctgttc
     3841 gactacatcc actatgaatt gccgatcgcc tatgcttcta aagcccaggc ggtcttctgg
     3901 attgggatac aaggcctgag cttttttctg ggctccagct tgggtgggct tttggtcgaa
     3961 ggcatcggcc ttgaccgaac ctatctccta ctgtctatcg ggctgttctg tgtcgccgca
     4021 ccgctgaccg ttgtcgcggt cgtcaagcgc ttcaagggtt ccgggatgaa aagaaaaacc
     4081 gcctaacgct tgttcgaaga caggtcgaaa gcccctttcc ggtttttagt gtgcgtcagg
     4141 ctttggggcg taatcaagaa aaaaggtcag aatcaaccgc gcgaattttc gtccgtgtag
     4201 tcattgtggc ataagaaagg caaaaagcgg atcgcggggt cgtaaaaacc acttaccccg
     4261 cgataacaca cacgagcatc gcggcgtaag cgccccttct tctcgcgacg gcgcgatacg
     4321 cttttgtata tgtccgaaaa cagggttcgc acagagaaca cagagggcac ggaggagtgc
     4381 caaaaactta aaacctgccg gactgaagtc cagcgttccc agggcgttcc caagcgaaaa
     4441 catcgcggcg taagaaaggc aaagggggat cgcgagactt cgtgggtttc cgaagtcagc
     4501 ccgccggtta acccgcgata acacacacct tcatcctcat tccatttttt gctacgcctc
     4561 gatcttatac ccgcactttt gccgtcgctt tggaagcgta gaaccatact ggcggaaggg
     4621 cgccgtcaac gtggagccgt gtcaccaaac cgtaagactt gatggatagc tatctctttt
     4681 ttgccgtgaa gttccccgtt ttcgggaagg cgcctccgct gctcaccgtc cccttaaggg
     4741 tgttgttgtc aatgaccgtc gctttgaacg tgatctctcc gttgtagaag gacttcccgt
     4801 tgaatgtcaa ttcgttgctg ctgacgattc gtccggtcac gttcgtcgtt ccgaaggtgt
     4861 ctttccaggt cagggtgatc gacgtttgac cgtgggtgat ttgagcgctc atcgtttcgt
     4921 cgaagatgcc gagcgtccaa gagaaatccc attggcccgt gaggttgtag cgcaaagaag
     4981 ggcttcccga ctgcgcccaa tcccacgtgc ggttttcgta cagaatgacc tttcgaccgt
     5041 catcggtcgt cgcgatcatc gggccacaga cgagtgtcgt cacggaaaaa acggtcagca
     5101 agatgaacag tatctttcgc ataaattcct ccttcaatca ctagactcga cattcaacag
     5161 atgaagttcg ggaagaacag aaaagcggtt cttcggggac aggatccagt ccagatcatt
     5221 tgaaaagccc aggcttctca aggtcatcgc gtggcgcacg tgatgtaagg tggctctaag
     5281 cattgactcg tcttggagtc ttggggccaa gaactcggca agcgtctttt cgtcggaaac
     5341 gggtgcttca tcgtaccgtt tcagagtgcg ccagaacagg gcgcccacgc cgaaatcctc
     5401 taaggacaag cggttgcggg ttcccgcgca gagaatcgtg acccgtcccc ttccgcgtcc
     5461 gtcgatgaca aagtttctct ccacccagga aacggcggcc tcaaagttga ccaaggagag
     5521 ggcgaacaga cgaccggtct ttttccagaa agccgcggcg gcccgtgtcc cattcgttgt
     5581 ggtcaacacg actgttttcc ccgtcaaaga gcggttttcc agctccgtgg gcgagttgcc
     5641 cccgtcaaag cccggagggg cgacccctcc ccgttcgccg tataagagca cttccggatg
     5701 gcgttctttc caacacaagg cggcctgaac ctccgcggtc aaaaaaaacc gttccgcgcc
     5761 ccgttgggcc aaggccacca ccatgctgct cgcccggtaa acgtcgatta aaaaacagac
     5821 gtcggagggt tcgtaaaggg tttcgcaacc cgtataatag aagggaatga ggtcgatctc
     5881 cattgcgcta tttcaacagc tcgaagtcga tttcaccgcg aacgtggtcg atgcctttga
     5941 cccgaacttc caagatatcc ccgatcttat agacctttcg cgtcctctcg cccaccaaga
     6001 tgttgcgctc ctcgtcgaag acgaagtagt cgtcgagcgt tgaaatcggg ataaggccgg
     6061 ggatgagttt ttcgggcact tcgacgaaaa ggccaaaccg tgcgacgttc gtgaccacaa
     6121 cttggtacag ttcggccagg ttccgactca cgtagttgac ttttttcagc gcgaccaaat
     6181 cccactccgc ctcgctggaa acgcgctccc gcaccgaaga ctgcctggcg atcttcgtca
     6241 gctgttccga gttatagggg gccggcctgt gttccatcca ccctttcagc atccggtgaa
     6301 cgacgaggtc cgggtatctc cgaatcggcg cggtgaaatg ggtgtagttc tccgaggcga
     6361 ggccgtaatg tccgatgttt tcctcactgt agagcgcgcg cttcatcgat cggatgacca
     6421 atcgctgtat gctgcttttc aacgggtggt cctggagggt ttcgagatac gcttggatgt
     6481 ccgaagagcg catctccttc ggcagcttct tctgtacgcc caacgcgagg aggtaccttt
     6541 ttagctggat cagtgtctca ggatccgggc gttcgtggac ccggtaaatg aaggcctctg
     6601 gcttgtcggc gaagatagag gcgacggttt cgttggccaa cagcatgaat tcctcgatga
     6661 aagactccgc tcgatcgcgt tttttgacca agatgtcgag ggcgttcccc acttcatcga
     6721 gcgggatctc gacctctccg ctctcgatct caataatcgc tcctctgctt ttccggttct
     6781 ctctgaggat tttggccagt tcgtccatca ggatcagggc ctgcccgatc tcagggtttt
     6841 tcgcgaacaa cttcgcgccc tcggaggggg tgtttccatt caagaagtca ttcactgcgg
     6901 tgtaagtcag tcgttttttg ctttttatca cgccttcgtg acattcgtac cgcacgaggc
     6961 ggccgttcga gtctacttcc atctccaaag agagcgtcaa ccgagaccgg ttctctatca
     7021 gagaacaaat cccgttggac agcgcgaggg gcaacatcgg gacgaccgtg tcgagcaggt
     7081 aggtgctggt acctcgtcgg taagcctcgc gatcgagctc cgaatccata gggacgtacg
     7141 cggatacgtc ggcgatgtgg actcccagca ggtaatggcc gttgtccagc ttttcgatcc
     7201 cgacagcgtc gtcgaagtct ttcgcggtct ctttatcgat ggtgaaaacg atctgattcc
     7261 ggtaatcctt tcggtttttg gtatctttcg ggaagatgcg atcgggtata cgtttagcct
     7321 cttccataac accctggggg aagtcttcgg gaaaaggcag cccgtgtttg acgatcacag
     7381 tgggtagatg gacccgcgga tcttcgatcg accccagata gtgggtgatc ttggccaccg
     7441 gggttttttt cttcgacggg taggatacga tgtgggcaat caccttcgaa cccagctcgc
     7501 taccgagccg gtctggtttt tccgctcctg tgaggtaata gcgaagtccc atgcgcttgt
     7561 cgtcgggaat gatgaagtag ttttgccctt cgcgcgccag gatgcccacc acgtctttct
     7621 ggttcctttg caggatcttc acgacctgcg cccgacgcca gtctttgtag aacccgctcg
     7681 gttttatttg taccagatcg tgatgaatgg cgtatttcga gtcttccgga aagatcacgt
     7741 actcgattcc gtcgctggcc gcgcaaaagg cgatatggcc atttcggctg aaatctagta
     7801 gccccgcaat tccgtgagaa gaggccttct ctccggactc ggacgcctcc tcgtcttcgg
     7861 gtattcgata acggttcgtc tcattctttt ctatcgcgcc ttcggcctcg agacgtttga
     7921 tgaaggcgcg aaattccgct ttttcgctct tggtgttggc gccgaaaact ctgtacagtt
     7981 ccctcaatag aaggggcacg gcttgtgttc tcaggtgctc caataccgcg cttttgtctt
     8041 tttgcattgg tttactcctt cgtcccgcgc tatgcgcaga actattgtta aaaataacag
     8101 atctttcctg ggggaacgac gttcactttg acattgtttt cggccatctt gcgttcgaac
     8161 ttttcgatcc gttcgcgaag ggagtcggta taccgactgg aaatgtggta aaggtagagt
     8221 tccttcggtt tcgctgccag tacgggctcg accaattctt ccagcgacgt atggttttcc
     8281 cctttccgct catcgatgtc gaggaaggtg cactcgtgga aaagcgtgtc ggtgttttcc
     8341 atacgcgaac ggggcatcac cagcccgtca ccgctgtagg tgaacagatt ttgatcgtag
     8401 tattcggata tctcctcaga gcctttgctc tgaatcgctt tacggatatc ggattgggac
     8461 atcgaccgat acggctcctt caagcgtttt cgtgtttcct tgatatgaaa gcccagggtg
     8521 cattcggttt ccatatgctt cgtggagaag gcttccaggt accggctctt cttcgccttt
     8581 cggtctaagg ccgagcgggt gggagcgccc tcctcttttg ggaagagcgg tagggtgaag
     8641 ccttcgtcga tctctcgaac gaggatatcg taccgcaagc gattgctgga ctccacgatg
     8701 aactccagat acttgcgtat tctggatgag ctgctgtgat aatatatttc gaggggcttt
     8761 tcacgttcgc ccatcgcgct gttccgggtg ttcaagaatc cccagagacc ggaaatgtga
     8821 tcggcgtggc cgtgagacag aaaaatgcgc tgaaccgcgt agatcttgtt ggtcagaaag
     8881 gtgctgacgc cctctccgca atcgaacata atccgatcgg gcgcgtagta gagccacgtg
     8941 gaatacatgg cctttgaaaa aacaatgatc tccaatctat tcctccgttt tctattctag
     9001 ggcgttttct cgaccttgat tttgagaact cctctgaccc ctcccgagaa cttcacgccg
     9061 acctcgtaca cccctatctc cttgatcggt gcggacaacg agatacaacg tttgtccacc
     9121 ggctcttcaa gcaccttttc cagttcttgc gcgatgtcgg cgttcgtgat cgaaccgtac
     9181 agcttcttcc cctcgcccgc tttggcgtaa atggtgaaaa actccttggt cagacggtcc
     9241 agtttctcct ggcttttctg ctgcatctta tcttgatgat ctttacgtat ctgcgcgatg
     9301 gattgggaat gcttgagggt cccttcggtc gcttctttca ccaacccctt aggaatgagg
     9361 aaattcctcg cgtaaccgtc ggcgacggtc ttcacttccc ctttcttacc tatttttgga
     9421 acgtcttgca gtaaaatgac tttcatcgtg agtcctcctt ccttcgattc ttgacgctta
     9481 atgcgtcctt atcaatcggt cttcgcatat ttcaatcttc gttattgttg ttgtggtctg
     9541 gccacgcacg cgcttcaaaa aactcgctaa caagaataat cgagttcgac acgccgtttg
     9601 atcccttcgt gaagctccag aatctgtgag agttccgtca cttcataggc ctcctgggat
     9661 aagacggttc tttcgacgag gcgcgtcagc tctccaaaag tcacctttcg ttgtaggaac
     9721 aagaggactg ccgcttcgtt cgctgcgttg agcgcgattc tcaaagagct aggcgcgtct
     9781 atgaccgctt gggccaaccg taacggggga tggactgccg tgtcggccgg tttcaggatc
     9841 atcggctcga agaggctgcg cggatcttcc acggcggttc cgttctcttc caaaaccgac
     9901 acccgttcgg ggtaggtcaa ggagtaggcg atcgggatcc gcatatcggg cggtccagcg
     9961 tggatcttga aatgcccgtc ccagtacgcg aggaccgcgt gaacgaaact gttgtgacag
    10021 aaagcggccg cgatcgtttc cttcggcaaa tcgaaaagga acttcgcctc gatcatctcc
    10081 agcgctttat tcatcaacgt ggcggaatcc accgtgatct ttgcgcccat ctgccagttg
    10141 gggtgtcgca ggacctgctc cacgctcgcg ttctctttac gcgactcgtc ccagtcgcgt
    10201 aaagcgcccc cggaagcagt gatatagacc ttgcgtattt ccttgcgatc ggtgcccaac
    10261 aaaagttgaa acagcccgct gtgctcgctg tcgaccggta tcaactcttt gcctaaggct
    10321 ttggccctct cgggaatcag tcgcccaccg agcacgacgg actctttgtt ggccaaacag
    10381 agcctcttcg aggtctcgag ggccttcaag gcataagaca gagagattcc gccggaagag
    10441 gcgatcatcg tcatatcggg gtgcagcgcg tccagcatct cttcgacggc gccgtaacgg
    10501 cgcgcggtct ccggtattcc cgttaaaggc ctttcgaaac aataacccgc gggtctgtaa
    10561 gttttcaact gttgaataaa tgagggatga cttcgggcgg ccaggccgac gacacgcatc
    10621 ccgtacccgc gcgcagacag gcgatcgatg acttgtagcg tctgggttcc gatggacccc
    10681 gtgctgccta tcacgacaca agtggcagtt ttttcgcgct gcgcggatgt gttcatcgat
    10741 aggctcctat tctacttccg atgtcgtgaa ggctgcgccc atccacgatc acaaaggggg
    10801 cgcggactcc gaatcgcaaa ggtcttgata atcgcaatac gcgcacaaag gccccttttt
    10861 tttcggaaag gcgttgtttt gagccgccag gaggacggtt tggcactttt ctttcaaggc
    10921 ggcctctgtc ctttggtttt gttcgtgcga aaaggggcgt tccacgagtt cgtcgtcctc
    10981 cagaaagtag aaaagtgtcg aatcgacttt caagccgtat tggtaggcca accaggagta
    11041 ggtgtgcagc tggagaaaat caatgaaccg ttcagagaat ttgccggtct tgaaatcgac
    11101 gatgcgaaac ccggtctgac gtttttcgat acggtcgata cggccgacga agatcaaatc
    11161 ctcacccagt ggcacatcca aaaacctttc ggtttcgtag gggcagctgg caaatggact
    11221 tttatagaag ttttccagca tcctcaggcc acgtatcccg tagatggctt cttcggaacg
    11281 ggacatttcc aacagccctt gttgacggca cttgttgcgt tcggccatcc atttctgtcg
    11341 gaaggtctga tgaagccact cgaggtcctc attttcggta gaaaacatca caccggcgac
    11401 gatctggccc gaagacttgc gttgtagggc tttttcgtag gcagcgatcg tgtcgtggac
    11461 gatttggcct aagaagagcg ccggcgaggt ttttgagggt acttgctgta cgtattgata
    11521 ccgaaacttg agcggacact ggtcgaaagc gctcagtttg gaataagaga aacgggtcat
    11581 atcggtccct cgaaacgttc gcccaaagac aataggcgtc cctgtgtggc ttgatatgta
    11641 gaaatcaaag atttaccagg aaattgaatc cgccccacga tcacgctacc cattccgcaa
    11701 gcaattcggg cgccctgttt cgtgatatcg agaatggtac ccggagtagg tttcgaagaa
    11761 gctgaggcag gcggtttttc ttctggcccg tcgtaaagcc ggcttccggc cagtttgacc
    11821 gtcacacctt tatatgcggt tcgggccaaa gggtccgcgt cgaaggcgcg gattcggtta
    11881 tgtacagctt gcgaggagtc cgcccaacgc acctcccatt cctctttctc cactttcgcg
    11941 gcataggaga gctcttgagc gggatcctgc tccgttaaat ggacggtcgc cggatcgaat
    12001 ccccgtaaaa aatcgaggag catcgggagg gcggtttctc tcatctgatg aacaagcccg
    12061 ctataggtct gatcggccga taccggcatc gttacgctcc gcgcgaccgg cccctcatcc
    12121 aaagccaagg acatcttcat caggcacacg ccggtttcat tgtccccgtt gagcaaacac
    12181 cgttgaatcg gcgccgcgcc ccggtatcgg ggcagcaacg aggggtgaag gttataggcg
    12241 ccgtacctgg gcagttccag cgccggctct ctcaacaact tcccataagc gatcaacaaa
    12301 atcaaatccg gctgataacg cgccagggat tcgaagcctt cgcctcggtt gatctgacgg
    12361 ggctgaaaga cggggacgcc gtattgtaac gccgtttgct tcacgggtgg ggcaaccctt
    12421 ttgttccctc tcgcgctgac ggcgtcctct ccggtaaaca cggcgactat gggaacccct
    12481 tcttggagaa gcggctccag gtagttggct gcgaactccg gcgtgcccaa gaaaacgacg
    12541 cgcctgagaa atggattgaa gaatccgctc attaaagggt ttccttcgct tttggattgg
    12601 attcgctcga tgcggggcgc ggttccacga aatcggcagc cattctcgga atcgctttgc
    12661 gttggatatc caggagcgtc cggtggagaa ggcgtcgctt ggccacgctg atatggtcga
    12721 taaacaacac tccgtttaaa tggtcggctt cgtgttgaaa gacgatggcc ggatacccct
    12781 ccaaccgctc ttcgacggtc tctccttcta gattttgata ccgaaccgcg atgctctgtg
    12841 ggcgctccac gtcctcgtag atctcaggaa ggctcaaaca cccctcttcg ttgattacgc
    12901 tctccttgct tcgccacgtg atctcggggt tgacgatcac tttccatccg ccaccgctgc
    12961 cgtcatcgat ggcgaagacc cgtttgggca ccccgatctg cacggcggcc aagccgaccc
    13021 cgtcgtactc gtacatcaac tcgaccaatc gagtcgcgaa acgccgaaaa tcctcgtcga
    13081 aggattccac tcgtttcgat ttcgatcgta aaatggggtc tccgtaatac cgtacgtgat
    13141 aggtcatgcc gagctcctca agcgctatcc cggatattgc cgacgtaatt gttcgaaggc
    13201 tttgtcgtcg ttcataaaca cgctcagcgg ggtgatcgag acgtatttgt tcatcactgc
    13261 gtaatagtct gactcaaatg aaggttgttc ctcaatcacg tctcccatca accagaagta
    13321 gtcgttaccg taggggtctt ggcgcttttc atagaaatcg ttgtatcttc gattactctg
    13381 tttcgtgatt ttaaaaccct tcagctccga aaggggtacc gtgggcacgt tgatgttcaa
    13441 agccccgaag ggggggatgg acttggggtc gaaatctttt aaaaaatcga cgagaaaggc
    13501 ggccgccgtt tcgtaggtgg actccttatg gctcacgtcg gagatggcga tagagggata
    13561 acccataatc gcgccttcca acgcgccgga gaccgttcca gagtaaggaa cgtccgtgcc
    13621 taagttggcg ccgtggttga tacccgaaac cacgaggtcg atcttgaagg gggccaactg
    13681 tagaatgccc agtttcacgc agtccgccgg cgtcccgctg gaggcgtacc cgaagaaccg
    13741 cccgttcaac gagacggact tcgcccacac aggcatccgc aaagtgatcg cgtgaccgat
    13801 cgcgctcctt tccgtatcgg gcgcgcagac cagggctgcg tgccccgcgt tcgtcagata
    13861 gtctttaagg acctttaatc cgggtgcggt gatcccgtca tcgttggtaa tcagtatatt
    13921 catacaaatc ctcttttcat cggtgatagg caccctcgcg gggcaccgtg tcatccgtcg
    13981 attataccac aaaggctttt tcgttgaacc gcgccgacaa cgccattctg ggaacacctg
    14041 ggaacgccaa aattcaagtt ggtatgcttt gaaagaaaaa agatgggttc gcacagagga
    14101 cacagagaac acagagagaa caggcatcgc ggcgtaagcg caccttcttt ttgcgacggc
    14161 gcgataccgt tttttatacg ctattcgcgg cgggttaaaa aacaaaaggt ggatcgcgag
    14221 acttcttggg cttccgaagt cagcccgctg gttaccccgc gataacccta cacggctggc
    14281 atcgcggcgt aagcgctttt tcttctcgcg acggcgcgat accccggttt tcgttcgttc
    14341 caaagattaa aaaacagggt tcgcacagag ggcacagata acacggagaa atgattgaag
    14401 atagttcgcg aggtcggctg aaagtcggtc cggctctctg ggaacgccct gagaacgcca
    14461 acgacgttcc caaagcgctc tctgtctgac cctcaaaacg tctcacatca gaagatgcct
    14521 tttcagctcc tcctcactct gcatttccat cagatccaag cccaaggtat cgagctggtc
    14581 tatcgtgagc gcttttagct tttcgtagta ccttgaaggg atttgtggga attttttgga
    14641 gatcaacttc caaagaagtt cttcccggcc ttcttctttg ccttttataa tcccttttgc
    14701 taatcccttt tccattcctt ctttcatacc tttttccatt cctttttcca ttccttcttg
    14761 cttaagccat tccgctatcg tttgcatcc
//
LOCUS       DAOPXN010000050        14721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4163_length_14721_cov_5.002796, whole genome shotgun sequence.
ACCESSION   DAOPXN010000050 DAOPXN010000000
VERSION     DAOPXN010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 14721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14721
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4163_length_14721_cov_5.002796"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..450
                     /locus_tag="PKM49_04210"
     CDS             130..450
                     /locus_tag="PKM49_04210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY82051.1"
                     /translation="MSIEQTKASIVIPVYRAERTLDRCLASVFAQTHSNLEVILVDDG
                     SPDSCPEICDSCAQKDERIRVIHQSNAGVGAARNAGMSAMSGAYLFFLEFRGNNMQGI
                     YPGC"
     gene            complement(770..1216)
                     /locus_tag="PKM49_04215"
     CDS             complement(770..1216)
                     /locus_tag="PKM49_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82052.1"
                     /translation="MVHIVYCDDKEKVLEKILDGTKTMVVRGAAGRKIPHSRVFEGET
                     LYFMKKGTAKISAKATVDSVTNLIKLSEDEIVATLAEPRLALTPKQKERWHKKCHYLV
                     GFTDVTPIGPHDFEKQSNMDDWLIVEKLEDVLVGTSIPYNYEKSKF"
     gene            complement(1242..1691)
                     /locus_tag="PKM49_04220"
     CDS             complement(1242..1691)
                     /locus_tag="PKM49_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HNY82053.1"
                     /translation="MDLDSLVASLARSDNQTAYENLKLLEAESARSNAVYRYFDTFIE
                     MLDHDNSYVRTRAIILISANAKWDTDYKIDENITRLLRHIEDVKPITARQCIQRLPEL
                     AKHKPELREDVLTALRKVHKREYAGSMQPLIHKDIALAIKRIESYTD"
     gene            complement(1761..2435)
                     /locus_tag="PKM49_04225"
     CDS             complement(1761..2435)
                     /locus_tag="PKM49_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNY82054.1"
                     /translation="MKNKVMDYTVRVFAQKLLGLLCYALGAGWVMSWGAWVYFGVGFG
                     LTALSITIVYRVNPVTLAERGKVLTDSPVWDKWLLGVFWVLYFFVIHLVAGLEAKNAP
                     DPAVLFWIGILLIIASAAIATAALAVNTYLESTARIQSERDQIVITKGVYGVVRHPTY
                     AAVLVSCVGIALVFPTPFVALTAAIIATLTVFRTYLEDNLLKKNLDGYIEYSRRIKYR
                     LIPFLW"
     gene            complement(2535..3122)
                     /locus_tag="PKM49_04230"
     CDS             complement(2535..3122)
                     /locus_tag="PKM49_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNY82055.1"
                     /translation="MPFFIVQHDITKIPADVIVNAANTDLLMGGGVCGAIFNAAGPEE
                     LQAACDKLAPIETGEAVITPGFNLPARFIIHTAGPIYQPSEKERCEQQLRAAYTNSLK
                     RAVENDCVSVAFPLISSGIYGYPKEEALRVAITAIRDFLTARDLTVFLVLFDASRFFI
                     DGNPIESIEGYVDARSLDFHELLSKKPREKGLGGV"
     gene            complement(3535..3885)
                     /gene="ssrA"
                     /locus_tag="PKM49_04235"
     tmRNA           complement(3535..3885)
                     /gene="ssrA"
                     /locus_tag="PKM49_04235"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3850..4497)
                     /locus_tag="PKM49_04240"
     CDS             complement(3850..4497)
                     /locus_tag="PKM49_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNY82056.1"
                     /translation="MRGYKNIVFDIGNVLIRYDPIGFLERSPYAEQKNEYIRHIFDAE
                     WWVELDKGTIDEETVFRHLYEQFPDLREGISYISNHWINEVFSLIEGTSQILTELKEA
                     GYRIYLLSNFGERGFQKVSKEYAFFEKVDGKIVSYDVKTVKPEREIYDLLLERYDLAP
                     HETIYIDDRPENLVVPEEYGILCHHFRNPETLADFLGRAGLSVYGGEKGSTGTED"
     gene            complement(4494..4964)
                     /gene="nusB"
                     /locus_tag="PKM49_04245"
     CDS             complement(4494..4964)
                     /gene="nusB"
                     /locus_tag="PKM49_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNY82057.1"
                     /translation="MESDWPEKQKSAGRRKLREAVFKVLFSVDFGNRNDTNEIGLILS
                     SLTEEEGGQFTGLEQEIVHYIESIYAHLEEIDEIIKLNILNWSWERLVAVDKSILRLA
                     TYELLYEKRVPIEVSLNEAVEIAKAYGTEKSGKFVNGILDVIAKKYSSEEKRAL"
     gene            complement(4966..5472)
                     /locus_tag="PKM49_04250"
     CDS             complement(4966..5472)
                     /locus_tag="PKM49_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82058.1"
                     /translation="MNKVILRWVTITGIAIFFISVGFWIVSFPFGWAKWLAENDWISA
                     LFRIFLTAVGILVFWWAHTKRERFLSVIQEEGLDASVSVGVFKTLINKVLEGFDGITL
                     EKVAFKKSKFANITDLVIELKVNDPYSLSEVLKEIHAKVESSIRENIGEMTGIHPSIK
                     VRGFEVKA"
     gene            complement(5477..5920)
                     /locus_tag="PKM49_04255"
     CDS             complement(5477..5920)
                     /locus_tag="PKM49_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HNY82059.1"
                     /translation="MEEEKMTVSPSQMKDENQPVQSKETQYGSVEIADSVILDLVNVA
                     LGKVEEIVKDQKKEKKAVAMDRKGNENTVSITVSTKLMLSKSIREVAEKIQRTIKEEV
                     EGMTGLIKVEKVTINVLDVALMLKEEPQIPTFSDDEPGEHPEKKG"
     gene            complement(5959..7047)
                     /locus_tag="PKM49_04260"
     CDS             complement(5959..7047)
                     /locus_tag="PKM49_04260"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNY82060.1"
                     /translation="MKEEIISRVDKLLASMAESSVDAVLIANEEHSTKNLRYFSNFTG
                     STGYLLISRDKQMIFTDSRYWEQTLAQSPLTLEKIKELRTRPFVARFFQNYKRIGFEA
                     KALTVDFFNGFEALLPPDVEWIPVDGMISEIRCSKSVLEKNKIVEANKIAIDAFKKVI
                     GELKVGMTEKEVAIALEFQMLKLGGEDKSFGTIVASGWRSALPHGAASDKKIEKGEFI
                     VFDFGCFLGGYCSDITRTIALGKPSDEMLEVYDIVKKAQNSAERAAKAGMSGKDIDTI
                     ARDIITNEGYGEYFGHGLGHGIGLEVHEEPRVSQINTNALPVGAVVTIEPGIYLPNRF
                     GVRIENDIFLGLETTEVLTVFPTDLIIV"
     gene            complement(7468..8058)
                     /gene="scpB"
                     /locus_tag="PKM49_04265"
     CDS             complement(7468..8058)
                     /gene="scpB"
                     /locus_tag="PKM49_04265"
                     /inference="COORDINATES: protein motif:HMM:NF016005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNY82061.1"
                     /translation="MNKLAMVEALLFASKRGIDLNQLSQIIESPKAEALSLLEELREK
                     YAQETHGVKLGQVGKLYSFYTKPEYAETVAKLVIRPMEKLTPSQLEVLAVVAKKGPVK
                     KSQIDEIRGKGSDNQVIELLAMGLVRRKRAKGPGRPFAYSVTELFYDLFHLSDEELNR
                     YKLPPEDEETVAGPELAADESENEEEPIDPEPNSVV"
     gene            complement(8055..8342)
                     /locus_tag="PKM49_04270"
     CDS             complement(8055..8342)
                     /locus_tag="PKM49_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNY82062.1"
                     /translation="MAVFLYTILILGTISFSSGVFLAFAAKKFEVKEDPLLKEVLAAL
                     PGANCGSCGFAGCEGFAKEILKGAVKADICIPGRKSGSPEKIKVILDNRPK"
     gene            complement(8354..8929)
                     /gene="rsxA"
                     /locus_tag="PKM49_04275"
     CDS             complement(8354..8929)
                     /gene="rsxA"
                     /locus_tag="PKM49_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNY82063.1"
                     /translation="MKVFTIFFAAILVNNFVLQKFLGICPFLGVTKKIDTATGMTMAV
                     IFVMILASLITWLLNGLLEALGIGFLTTIAFILVIAVLVQFVEIFLKKSSPALYQALG
                     IYLPLITTNCAILGLTLINVQNQYSLLETVFNSLGAGVGYGMSMILFAGIREQLDLVE
                     IPEPFKGFSGALVTAGLMSMAFMGFNGMIKL"
     gene            complement(8926..9612)
                     /locus_tag="PKM49_04280"
     CDS             complement(8926..9612)
                     /locus_tag="PKM49_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNY82064.1"
                     /translation="MTDKTLGSETQKKFFDFSILTSGIFANNPTFIQVIGMCPTLATT
                     TNAINGLGMGVATLFVMVMSNLMLSVVRKWTPDKIRIPIFVIIIAGFVTIIDLLMHAF
                     TYDLWKTLGIFIPLIVVNCIIMARAEQFASKNNVVDSILDGIGNGIGFIGALVLVGSI
                     RELLGNGTIFGVTVWGEAFRVFLVILPPGAFITLGLLLALFNWAGIERKKAEKKRAEA
                     LKAARAGEKA"
     gene            complement(9612..10328)
                     /locus_tag="PKM49_04285"
     CDS             complement(9612..10328)
                     /locus_tag="PKM49_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNY82065.1"
                     /translation="MGNYVKTGLILMAFTLVVGVLLAGIYVLTKEPIEKADLSNQLRA
                     MKTVLTDPNSGRSVVPEQEIPTSTAQLEKMIWRKADSETVFSSSVWKGAIYSPIYHIK
                     STEGKEIAIVSAGATGYGGDVKILAAFIQAEKGFDLFRLEVMDFSQETPGLGAKISEE
                     GVKARFFDFTTENLERGLKVDKDTGIGSLSSPAGIREAKKDGIIYVSDLMTGATITPR
                     AVANAVNTITEYLSAEWGEK"
     gene            complement(10321..11307)
                     /locus_tag="PKM49_04290"
     CDS             complement(10321..11307)
                     /locus_tag="PKM49_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNY82066.1"
                     /translation="MATVLSTSPAPHIRVKDTVPKIMVDVLIALVPAVLFAVYAFGWT
                     ALWLCVIGALSGELIEYAILRFIRKKKKTRPDGSAAVTGLLLAMNVSGSFPWYLLILG
                     VLVAIGIAKHTYGGLGNNPFNPALIGRVFLFVSFPVQMSTWAPLQNAVSTVVTSATPL
                     GVLKQKGFEEAARHISTLDMFIGNMQGCLGEVSALALIIGFAYLLWRKRVNPIIPLAY
                     LGTVFLFSSLFFWINPNLFGSPIFHLLSGGLMLGALFMATDMVTSPATMKGAFIYGVG
                     CGVLTMLIRCFGALPEGVSFSILIMNACKPLIDKISRPKPFGYVAPEVKKNG"
     gene            complement(11307..12635)
                     /gene="rsxC"
                     /locus_tag="PKM49_04295"
     CDS             complement(11307..12635)
                     /gene="rsxC"
                     /locus_tag="PKM49_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNY82067.1"
                     /translation="MAILSFSGGIHPPDRKELAKDQPIREAPIPKTFYLFVNQNSGKP
                     PALTVKKGDAVKTGQKIAQAQGPVSVPLHSPVTGIVSDVKKMMHPVTQKPEELLVIDR
                     ETDAYDFLEPLTGWETYDTEKCHTLLYGRIKEAGIVGLGGATFPSHFKYVEAMKKPVD
                     YVILNGAECEPYLTIDYRLMVEHPAEIVKGLEILLKICGAKHGYIGLEDNKPEAYEAL
                     RKHANGRIEPVLLKTKYPQGGEKQLVYALTKRAVPVGGFPFHVGSIVHNVATAFAIYE
                     AVEKGKPLIDRGLTITGEGISKPSNLWVRLGTPIQELIDYVGGLKSEGGKVILGGPMM
                     GIPIKDLALPVMKGTSGILVLPPEMVQEIDEETCINCGKCLMACSLKLEPNFLVKLVK
                     AKKFDEAYKYGVLNCCECGSCAFVCPSKIEHVKHFKLAKKLHATLKGGRS"
     gene            complement(12658..13680)
                     /locus_tag="PKM49_04300"
     CDS             complement(12658..13680)
                     /locus_tag="PKM49_04300"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HNY82068.1"
                     /translation="MRVFLFGYYGFENFGDELLLRSALKLLKDLPIERIDMLFPQKGS
                     RKVFGLKIKQIPRNSPFGILHALKACDIVIGGGGGIFQDETSAKSFSYYDFLVSTAFR
                     HKKPVYLLGHGIGAIRNPRNYRRLRKILAHPLCVGYFRDAISARYAAHFSALHSRGSD
                     LAYGTLSNREKIRTTPDKIGLMLKYPVKAIEEFIEPLKLEGYKAIDLLVSFPKEELIQ
                     AERNAAVLSKDFFVRMPQTTGGQLIDDISSCSLLVTERLHGVIVASYFGIPFITRSSF
                     KARAFLRDFKAYRAFYDDFNRMEVWGAMTELKKIDFHNQNAEFTRSNLEKYQKMVHSF
                     TNRFLK"
     gene            13905..>14721
                     /locus_tag="PKM49_04305"
     CDS             13905..>14721
                     /locus_tag="PKM49_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNY82069.1"
                     /translation="MIQGIQWLKKWKIAMGSLFLFTAIALATIGGDPFEPEVSDFSQL
                     AWTEAFSLLHQRLEEEYAFTEWKGIQWNTLWERSYARIERAQEKGDFESYYLALREYL
                     HVIPDGHVSCTNLIEIDEKHVGGGFGFSIAKVADGQLIITWVDAKSAAWEAGIRPRAE
                     ILEWNGESAKPAFDRVSTAFAGNSATDKDLDLKRTWYFVRAPVGAVVTMAFANEADAE
                     PKTVCLRAYEDEGVTFKRSYPSAILSDGVRDAFIDKPNPDPLPESMVETALLED"
BASE COUNT         3302 a         3971 c         3684 g         3764 t
ORIGIN      
        1 cggatgcgga tccgataccg agaggtgtat gatcaggtga tgggcgaaaa ttgactttcc
       61 ctgtgcggcc ttctctggct cgattccgcc cgttgtcaga ccgcccacgc cgctgagtgg
      121 aggaatcgga tgagcataga acagaccaag gccagcatcg tcatacccgt ttaccgggcg
      181 gagcgcacgt tagatcgctg ccttgccagc gtgttcgcgc aaacccattc gaatctcgag
      241 gtgatactgg ttgacgacgg ctctcccgac agttgcccgg agatttgcga ctcttgcgcc
      301 cagaaagacg aacgtatccg cgtgatccat cagtcgaatg ccggagtcgg cgctgcgcga
      361 aacgcgggga tgtctgcgat gtcgggcgct tacctgtttt tcttagagtt ccgtggaaac
      421 aatatgcaag ggatctatcc cggttgctga gccgaggcgc gtacacgaaa gcctctatga
      481 ttttatagtt gcgtttcgag gttatagaac gccggtggcg ctaaggcgct cctttctagg
      541 agcgccggac tttggtccgg catgtctttg atatttccac taagagctct ttttgtagga
      601 gcaaccgatc tacttttcgc ctatctcgtt tttcgccttc cctccgtgcc ctctgtgccc
      661 tctgtgccct ctgtgccctc agtgtgaacc ctgttttcca agacatacca acttaaaagt
      721 tggctctccc aggcgttccc acgcaacacc gatcaattgg tttgcgctgc taaaatttcg
      781 atttttcgta gttgtacgga atgctcgtgc ccacgagcac atcctcgagt ttttctacga
      841 tcagccagtc atccatattg ctttgtttct cgaagtcgtg ggggccgatc ggtgtgacat
      901 ccgtaaaacc gacgaggtaa tggcattttt tatgccatcg ctctttttgt ttgggggtca
      961 gcgccagacg aggttctgcg agcgtggcga cgatctcgtc ttcggagagt ttgatcaggt
     1021 tcgtcacact gtcgaccgtc gcttttgccg atatcttcgc ggtacctttt ttcataaaat
     1081 acagcgtctc tccctcaaat acccggctgt gtgggatctt gcgcccggcg gcgccacgga
     1141 ccaccatcgt ctttgtaccg tccagtatct tctccaacac cttttctttg tcatcgcaat
     1201 acacaatgtg caccatgccg atatgactcc tttccgagcg gttagtctgt gtaactttcg
     1261 attcgtttga tagctaaggc gatatctttg tggatcaatg gctgcatact gcccgcgtat
     1321 tctctcttgt gcacctttcg gagggcggtg agaacgtctt ccctgagctc cggtttgtgt
     1381 tttgcgagtt ccgggagccg ttggatgcac tgcctggcgg tgatcggctt gacatcttcg
     1441 atatgtcgga gcaaacgggt gatgttttcg tcgattttat aatcggtatc ccatttcgcg
     1501 ttcgccgaaa tcaggatgat tgcccgtgtt ctcacgtaag aattgtcgtg gtcgagcatt
     1561 tctataaagg tatcaaaata ccgataaacc gcgttggaac gcgcgctttc ggcttccagc
     1621 agtttgaggt tttcataagc tgtctgatta tctgagcgag caagagaggc caccaaagag
     1681 tcgagatcca tgttttttcc tccaccatcc ctatcgatgc cgaatcaatc aggcgcgatc
     1741 caccccgtat ccaaaagccc tcaccagaga aaagggatca gccgatactt gatgcggcgc
     1801 gaatattcta tatagccatc gagatttttc ttcaacaagt tgtcctcgag ataggtgcgg
     1861 aagaccgtga gcgtcgcgat tatcgccgcg gtcaacgcaa cgaagggggt cgggaacacc
     1921 aaggcgatac cgacgcagga gaccagcacg gcggcgtaag tgggatgtcg gacgacccca
     1981 tacacgcctt tggtgatcac gatttgatca cgctccgact gtattctggc ggtcgattcc
     2041 aggtaggtgt tcacggcgag cgcggccgtg gcgattgcgg ccgaagcgat gatcaatagg
     2101 atcccgatcc agaagagaac ggcagggtcc ggggcgtttt ttgcttcaag gccggccacg
     2161 aggtgtatga cgaagaagta taggacccaa aaaacaccga ggagccattt atcccatacc
     2221 ggtgagtccg tgagcacttt tccgcgttcg gccaaggtga ccgggtttac ccgatagacg
     2281 atcgtgatgg acagcgcggt caggccgaat ccgacgccaa agtataccca ggctccccac
     2341 gacattaccc atccggcgcc caacgcataa cacaacagac cgagcagctt ttgcgcaaag
     2401 accctaaccg tgtagtccat caccttgttc ttcaaacgcg acctcctaat ttcgtatggc
     2461 ccggtttttc atcacacctt gtcgtataaa atctacaccc tcatcctcga gtctgcctgt
     2521 ttcatctttg ctcatcatac gccgcccagc cctttctccc gtggcttttt cgataggagt
     2581 tcgtgaaagt caaggctgcg agcgtcgacg tacccttcta tcgactcgat cgggtttccg
     2641 tcgatgaaga agcgtgaagc atcgaacaag acaaggaaca cggttaagtc gcgggcagtc
     2701 aggaaatccc gaatggcggt aatcgccacg cgtaaggcct cttctttcgg gtaaccgtag
     2761 ataccgctgg agatcagagg aaaggcgacg cttacgcagt cgttttctac tgctcgcttc
     2821 agcgagttgg tgtaggcagc gcgaagttgt tgctcgcaac gctctttttc ggaaggctga
     2881 tagatggggc ccgcggtgtg aatgatgaac cgggcgggca ggttgaaccc cggcgtgatt
     2941 acggcttctc cggtctcaat cggggcgagc ttatcgcaag cggcttgtag ttcctccggt
     3001 ccagcggcgt tgaaaatggc tccgcacacg ccgccaccca ttaagagatc ggtattcgcg
     3061 gcattgacga tgacatcggc cggtatcttc gtgatatcgt gttgaacgat gaaaaaaggc
     3121 atctcttccc cccggaactc ttccccattt tgtagcgcgg ctaagcaacc tctcagggtt
     3181 agtgtaccac atctcgagcc tttctcggaa accctgagag tgcagtcgga acgccgtcga
     3241 agcgccgcca gagcggcgga ctttagtctg acatgttttt tccgaaccta taaaattaca
     3301 aaaaggagat ttcccaccga atgcctctct gggagcgccg ctcccagaga ggcaaggccc
     3361 tgccgaacta aagttcggcg ttcccaggca aagcacatac cgcactaaag ttcggcgttc
     3421 ccagcggcgt tcccagctgc gggcagggga aatccggcat agttttactt ttcaaaaacc
     3481 ccctttcaaa aaactatggc acgccaaggg ttgacgtgcc atagggtgtt tgattggtgg
     3541 aggcgggggg aattgaaccc ccgtccgaaa acagttcata acaggtttct acgtgtgtag
     3601 cgtctgatcg atgtcgggtt ttcgcagttc agacgcaagc tgctaaaacc ccaatcccta
     3661 cggtgtcctc ggatcgcttg ggagggcgat cgagtgagcc cgaattgctc acgctctcgc
     3721 gaaacgtctc gagctaaacc tttcgcagaa cggcaactta cattaagctg ctaatgcaaa
     3781 agttggttcg acatttatcg atgttccgcc tttttaatga ggcatgcgga gacctcaaca
     3841 cgctgcctgc taatcttccg tccccgtcga acccttttcg cccccgtaaa cgctcaggcc
     3901 tgcgcggcca agaaaatccg ccagggtttc gggatttctg aaatgatggc agagtatacc
     3961 atactcttcc gggaccacca agttttccgg tcggtcgtcg atgtagatcg tctcgtgcgg
     4021 ggccaaatcg tatctttcta gaaggaggtc gtatatctcc ctctcgggtt taacggtttt
     4081 cacatcgtat gagacgatct ttccgtccac tttttcgaaa aacgcatact ctttcgaaac
     4141 cttttggaat ccacgttccc cgaagttcga caacagatag atccggtaac cggcctcttt
     4201 tagctcggtt aagatctgac tcgtcccttc gataagggaa aagacctcgt ttatccaatg
     4261 gttggaaatg taagaaatcc cttctcgaag gtcggggaat tgttcatata aatgacggaa
     4321 aacggtttct tcgtcgatcg tacctttatc cagttcgacc caccattccg catcaaagat
     4381 atgacggatg tactcgtttt tttgttccgc gtaaggactg cgttctagaa acccgatcgg
     4441 gtcgtaccga atcaggacat tcccgatatc gaagacgatg tttttgtatc ccctcatagc
     4501 gcccgtttct cctccgatga atattttttt gcgatgacat ccaggatacc attgacgaat
     4561 tttccgcttt tttccgttcc gtaagctttg gcgatctcga ctgcctcgtt gagagagact
     4621 tcgataggca cgcgcttttc atagagcagc tcgtaggtgg ctaaacgtaa gatgcttttg
     4681 tcgaccgcca ccaaccgctc ccacgaccag ttcaatatgt tgagcttgat gatctcgtcg
     4741 atctcttcca aatgggcgta gatactctcg atgtagtgta cgatctcctg ctcgagcccc
     4801 gtgaattgcc ctccttcttc ttctgttagg ctcgacagga tcaagccgat ttcgttggta
     4861 tcgtttctgt ttccgaaatc tacggagaaa agcactttga acaccgcttc cctgagtttc
     4921 cttctccccg cgcttttttg cttttctggc cagtcagact ccattttatg cttttacctc
     4981 gaaacctctc actttgatcg acgggtgtat tccggtcatt tccccgatgt tttctctaat
     5041 ggacgattca accttcgcgt ggatctcttt caacacttcc gataacgagt aagggtcgtt
     5101 tacctttaac tctatgacca aatctgtgat gtttgcgaat tttgattttt tgaaggccac
     5161 tttttctagt gtgatgccgt caaacccttc aaggaccttg ttaataagtg ttttgaagac
     5221 tccgaccgag acggaggcgt ccaggccctc ctcttgaatg accgacagga atcgctctcg
     5281 tttcgtgtgc gcccaccaaa acactaggat gccgaccgcc gtcaaaaaga tgcgaaataa
     5341 cgcgctgatc caatcgtttt ctgctaacca tttggcccaa ccgaaaggga aggacacgat
     5401 ccaaaaaccc acactgataa aaaaaatcgc tatgcctgtt atcgtcaccc atctcaggat
     5461 aactttgttc atgtcgttag cccttttttt cggggtgctc accgggttcg tcatcggaaa
     5521 aggtgggtat ctggggttct tctttcagca ttaacgccac gtctagcaca ttgatcgtga
     5581 ccttttcgac cttaatcaag ccggtcatac cctccacttc ttccttgatc gtcctttgta
     5641 tcttttccgc tacctcgcga atgcttttac tcagcatcaa tttcgtgctg acggttatcg
     5701 acacggtgtt ttcgttgcct tttctatcca tcgccaccgc ttttttctct ttcttttggt
     5761 ctttgacgat ttcttctacc ttcccaagcg cgacgttcac cagatcgagg ataacggaat
     5821 cggcgatctc tacggatccg tactgggttt ctttgctctg caccggttgg ttttcgtcct
     5881 tcatttgaga aggagatacc gtcattttct cttcttccat gttgtcacct ccgcattttt
     5941 ccttaattgt tttggaattc aaacaatgat taaatccgtc ggaaagacgg ttaaaacctc
     6001 ggttgtttcc aacccgagga aaatatcgtt ctctatccga acgccaaacc ggttaggtaa
     6061 ataaatcccc ggttcgatcg tgacgaccgc tcccacgggt agcgcattgg tgttgatttg
     6121 cgagacccga ggttcttcgt gcacttcgag ccctatccca tggcccaagc catgcccgaa
     6181 atactccccg taaccctcat tagtgatgat gtcgcgagcg atcgtgtcga tgtctttccc
     6241 gctcatccct gccttcgcgg cacgctcggc gctgttctgc gcttttttga cgatatcgta
     6301 gacctctaac atctcatcgc tgggttttcc gagggcgatg gttcgggtga tatccgagca
     6361 atacccgccc aaaaaacagc cgaagtcgaa tacgataaac tccccttttt cgatcttttt
     6421 atccgaggcg gccccgtggg gtaacgcgct ccgccaaccc gacgcgacga tggtgccgaa
     6481 agatttatct tccccaccga gcttcaacat ttgaaactcc aaagcgatcg cgacctcttt
     6541 ttcggtcatc cctaccttca attcccctat caccttttta aacgcgtcga tcgcgatctt
     6601 gttggcctcg acgatcttat tcttttcgag gacggactta gagcagcgga tctcggagat
     6661 catcccgtcg acgggtatcc attccacatc ggggggcaga agcgcttcaa aaccgttgaa
     6721 aaagtcgact gtgagcgctt tcgcttcgaa tcctatccgt ttgtagttct ggaaaaagcg
     6781 agccacgaag ggacgtgtgc gtaattcctt tatcttttcg agggtaagcg gtgactgagc
     6841 gagggtttgt tcccaatagc gggagtccgt aaagatcatt tgtttatccc gcgaaatcaa
     6901 caaataacct gtggaaccgg tgaaattact gaaatagcga agatttttcg tcgaatgctc
     6961 ttcgttcgct atcaacacgg cgtcgaccga cgactcggcc atcgatgcca gaagcttgtc
     7021 gacacggctt atgatctctt ctttcatctc tatcctccgt ttatcactgt tgaaagatta
     7081 taacacatct tctattagta aagtcgcgag tgtgcgtgag tattatattg aggcgcaaaa
     7141 aaaagataag aggaggagat ccgccctttg cctcttctac gccacgatag gcccgtacaa
     7201 acgcgtatcg cgccgtcgcg cgaagaagag gcgcttacgc cgcgatgccg tgtgtgttat
     7261 cgcggggtaa gtggttttgt aggaccccgc gatccgccct ttgccccttc tacgccgcga
     7321 tgactacact tctatttcgc caacattttg gaatgctccc ttgagaacct caagtttctg
     7381 agatgttata gtttcaaagt tgcatcttcc gcaaaccgag atggtttaaa actggcgaag
     7441 ggcttcttct caatcggata tatgtttcta cacgactgag ttgggttcgg ggtcaatagg
     7501 ctcttcttcg ttctcagact catccgcggc gagctccggc cctgcaacag tctcttcgtc
     7561 ttcgggtggt agcttatacc gattcagttc ttcgtcggac aggtggaaga gatcgtagaa
     7621 caattcggtg acgctatagg cgaaaggcct tccgggccct ttcgcccgtt ttcggcgtac
     7681 cagccccatg gcgagcagct cgatcacctg attgtccgat cctttccccc gtatttcatc
     7741 gatctgcgat ttcttcacgg gtcccttttt cgcaacgacc gcgaggacct ccaattgact
     7801 cggcgtcagc ttttccatcg ggcggatcac cagtttggcc acggtttccg catactccgg
     7861 cttcgtgtag aacgaataga gcttcccaac ctgccccagc ttcaccccgt gcgtttcctg
     7921 agcgtatttt tcccgaagct cctcgagcag actgagcgct tcggctttag ggctttcgat
     7981 gatttgagag agctgattca gatcgatccc tctttttgag gcgaacagga gggcttcgac
     8041 catcgccagt ttgttcattt cggtcgattg tccaggatga ccttgatctt ttccggtgag
     8101 cccgatttac gaccgggtat gcatatatcg gcctttacag ctcctttgag gatctcctta
     8161 gcgaatcctt cacaccccgc gaaaccgcaa ctgccgcaat tggcgcccgg taaggcggct
     8221 agcacttctt tcagcagagg gtcttccttc acttcgaatt ttttcgccgc gaacgccaag
     8281 aagacccccg acgaaaaact gatcgttccc aatatcagga tcgtgtataa aaagacggcc
     8341 atagcgcatc acctcacaat ttgatcatac cgttgaaccc catgaaggcc atcgacataa
     8401 gtccggccgt aaccaaagct cccgaaaacc ctttgaaagg ttccgggatt tccaccaagt
     8461 cgagttgttc tcgtatcccc gcgaagagga tcatagacat cccatatccc actccagcgc
     8521 ccaaagaatt gaagactgtc tcgagcagcg aatattggtt ttgcacgttt ataagggtca
     8581 aaccgagtat ggcgcagttc gtggtgatca gcggtaagta gatgcccaag gcttgataca
     8641 acgccggtga gctcttcttc aagaatattt cgacgaactg aaccaaaact gcgatcacga
     8701 gaatgaaggc gatggtggtt aaaaacccga tgcccaaagc ctccaacaac ccgttcagca
     8761 gccaagtaat caggctggcc aaaatcatga cgaaaatgac cgccatcgtc atacccgtcg
     8821 ctgtgtctat ctttttcgtg actcctaaga acggacagat gcctaaaaat ttctggagca
     8881 caaaattgtt caccagtatg gctgcgaaga agatcgtaaa gactttcatg ctttctcacc
     8941 cgcccgagcc gctttcagag cttcggctct ctttttttcc gctttttttc tttcgatccc
     9001 ggcccagttg aagagcgcga gcagtaggcc caacgtgata aaggcgcccg gtggaaggat
     9061 cacgaggaaa actcgaaagg cttctcccca aacggttacg ccgaagatcg ttccgtttcc
     9121 taaaagttcg cgtatcgacc cgacgagcac caacgcgccg atgaatccga taccgttgcc
     9181 gataccgtct aagatggaat ccacgacgtt gttcttgctc gcgaactgtt ccgcgcgcgc
     9241 cataatgatg cagttcacga cgatcagcgg gataaagatg ccgagtgttt tccaaaggtc
     9301 gtatgtgaac gcgtgcatca acagatcgat gatcgtcacg aaacccgcga tgatgatgac
     9361 aaaaatgggg attcgtattt tatccggggt ccatttccgg accacagaca gcataaggtt
     9421 ggacatcacc atgacgaaga gcgtggccac tcccatcccc aacccgttga tcgcgttggt
     9481 tgtcgtcgcc aacgtcgggc acataccgat cacctggata aaggtcggat tgttagcaaa
     9541 aatgccgctg gtaaggatcg aaaagtcaaa aaatttcttc tgcgtttcgg aaccgagcgt
     9601 tttatcggtc attatttctc accccattct gcgctgaggt actccgtaat cgtattcacc
     9661 gcgttcgcca ccgcgcgggg cgtgatcgtg gcgcctgtca tcaggtcact cacatagatg
     9721 atgccgtcct tctttgcttc tctgatcccc gcgggcgaag acagggagcc gattcctgtg
     9781 tccttgtcca ccttcaaccc tcgctcgagg ttttccgtgg tgaaatcgaa gaagcgcgcc
     9841 ttcaccccct cctctgagat ttttgcccca agccccggag tttcttgaga aaagtccatc
     9901 acctctaacc ggaaaaggtc gaatcccttc tcggcctgga taaacgcggc caggatcttg
     9961 acatcgcccc cgtatccagt tgccccggcg gatacgatcg cgatctcttt gccctctgtg
    10021 gacttaatat gatagatagg gctatagatc gcccctttcc acaccgagga cgaaaaaacc
    10081 gtttcactgt cggccttgcg ccatatcatt ttttccaact gggccgtcga tgtcgggatt
    10141 tcctgctcag ggacgaccga tcgaccggaa ttcgggtccg ttaaaacggt tttcatcgcc
    10201 ctcagttggt tgctgaggtc ggctttttcg ataggctcct tcgtgagcac gtagatgccg
    10261 gctaacaaca cgccaacgac caacgtaaaa gccatcagaa tgagtccggt tttcacgtag
    10321 ttacccattt ttcttcacct ccggagcgac atagccgaaa ggtttcggtc tagaaatctt
    10381 gtcaatcaag ggtttgcacg cgttcatgat gagaatggag aaagagaccc cttcgggtaa
    10441 tgcgccaaaa caccgtatga gcatcgtcag gactccacaa ccgaccccgt agatgaaggc
    10501 gcctttcatc gtggccggag aggtgaccat atcggtggcc ataaacagcg cgccgagcat
    10561 aagcccgccg cttaataagt gaaagatcgg agagccaaat aggttcgggt tgatccagaa
    10621 gaatagggaa gagaagagaa acaccgttcc gaggtaggcg aggggaatga tcgggttcac
    10681 acgtttcctc cacaaaagat aggcgaatcc gatgattaac gccaaagcgc tgacttcccc
    10741 gaggcagccc tgcatgtttc cgatgaacat atccagcgtg gagatatgcc tggcggcttc
    10801 ttcgaagccc ttttgtttca ggacgcctag gggtgtcgcg gaggtgacca ccgtggagac
    10861 tgcgttttgg agtggcgccc aagtcgacat ttgaacaggg aaagaaacga aaaggaagac
    10921 gcggccgatg agcgcggggt tgaacgggtt gtttcctaac ccgccgtagg tgtgcttcgc
    10981 gataccgatt gctacgagga cgcctaagat cagcaagtac cagggaaagg aaccgctgac
    11041 gttcatcgcc aggagcaggc ctgtcacagc cgcgctgcca tcggggcggg tttttttctt
    11101 ttttcttata aaacgcagaa tcgcgtactc tatcagttct ccgctcagcg cgccgatcac
    11161 gcaaagccac aaagcggtcc acccgaaggc gtacacggca aagagaaccg cgggaactaa
    11221 cgcgatcaac acgtcgacca ttattttcgg aaccgtgtct tttacacgaa tgtgaggtgc
    11281 aggagaagtg ctcagaaccg tggccattat gacctccctc ccttaagggt ggcatggagt
    11341 ttttttgcca atttgaaatg cttcacgtgt tcgatcttag aggggcatac gaaggcgcag
    11401 ctgccgcatt cgcagcagtt caggacgccg tatttgtagg cctcgtcgaa ttttttcgcc
    11461 ttcaccagct tcacgaggaa gttgggttcc aatttcaaag agcaggccat cagacatttt
    11521 ccacagttga tacaggtctc ctcatcgatc tcttgaacca tttccggcgg taaaaccagg
    11581 ataccggagg tccctttcat cacgggcagc gcgaggtctt tgatcgggat gcccatcatc
    11641 gggccgccga ggatcacttt tcccccctcc gacttcagac cacccacgta gtcgatcagt
    11701 tcttggatgg gcgtgccgag tcggacccag aggttgctgg gtttggagat tccttcgccc
    11761 gtgatcgtca gaccgcggtc aatcaacggc ttcccctttt ccacggcctc gtagatggca
    11821 aaagccgtcg cgacgttatg gacgatcgac cccacgtgga atggaaaacc accgaccgga
    11881 acggcccttt tggtgagcgc gtagaccaac tgcttttcac cgccttgcgg gtatttggtc
    11941 ttcagtaaca ccggctcgat tcgtccgttc gcgtgtttgc gcaacgcttc gtaggcttcg
    12001 ggtttgttgt cctctaagcc gatatacccg tgcttcgcgc cgcatatctt caacaggatt
    12061 tccaagcctt tgacgatctc tgcgggatgt tccaccatca accgatagtc gatcgtcagg
    12121 tagggctcgc actccgcgcc gttgaggatc acatagtcca ccggcttttt catcgcctcg
    12181 acgtacttaa aatgcgaagg aaaggtggcg ccacccaaac cgacgatccc ggcttctttg
    12241 atccgcccat atagcagcgt gtggcatttt tctgtatcat aggtctccca gccggttagg
    12301 ggctcgagaa agtcgtatgc atccgtttct ctgtcaatta cgagcaactc ctcgggtttt
    12361 tgcgtgaccg gatgcatcat ctttttaaca tccgacacga taccggtcac gggcgagtgg
    12421 agcggtacgg agacgggtcc ctgggcttgc gcgatcttct gtccggtctt tacggcatct
    12481 ccctttttca ccgttagcgc cgggggtttt ccgctgttct ggttgacgaa caggtaaaaa
    12541 gtttttggaa tcggagcttc tcgaatcggt tggtcttttg ccaattcctt acgatcggga
    12601 gggtgtattc ctccgctgaa agataggatc gccaagatat cttctccttt cgttcttcta
    12661 tttcaagaat ctgttggtga atgaatggac catcttctgg tatttttcca agttagagcg
    12721 cgtgaattcg gcattttgat tgtgaaagtc gatttttttc agttcggtca tcgcgcccca
    12781 gacctccata cggttgaagt cgtcatagaa cgcccgatag gctttaaagt cccgtaaaaa
    12841 cgcccgcgct ttgaaactcg aacgcgtgat aaagggaatc ccaaaatatg aggctacgat
    12901 gactccatgc aatctctctg tgacgaggag cgagcacgag gagatatcgt cgatcagctg
    12961 tcctcccgtt gtttggggca tacgtacgaa aaaatccttt gacagaacgg cggcgttccg
    13021 ttctgcctgg atcaactcct cttttggaaa ggaaaccagc agatcgatcg ctttgtagcc
    13081 ctcgagtttt aagggctcga tgaattcttc gatcgctttg accgggtatt tgagcatcaa
    13141 cccgatcttg tccggggtcg tccggatctt ttcgcggtta gaaagcgtac cgtaggcgag
    13201 gtccgaacct ctggaatgca gggcagaaaa atgggcggcg taccgggcgg aaatggcgtc
    13261 ccggaagtat ccgacgcaga gaggatgcgc cagtattttt cgcaagcggc gataattacg
    13321 tgggttacgt atagcgccta taccgtgtcc taagagatag accggttttt tatgtctgaa
    13381 ggcggtcgat actaaaaaat catagtaaga gaaactcttc gcgctggttt cgtcttgaaa
    13441 gatcccgccg ccgcccccga tgacgatatc gcacgccttc agggcgtgaa ggatcccgaa
    13501 cggcgagttc ctcgggattt gcttgatctt caacccaaac acttttcgcg accctttttg
    13561 tggaaacaac atatcgatgc gttcgatggg aaggtctttt aagagcttga gcgcgctcct
    13621 caacaagagt tcgtccccga aattttcaaa gccgtagtaa ccgaatagaa atacccgcat
    13681 caacagttgt ctccccgtct tttttcaacg aaattataac acgtttaagg gaaattcggt
    13741 gttctcggtt ttggcgtgat aaaggggagt gtatacccat cagaagcccg ccctcttttt
    13801 ttccccctcc gtttggtgta aaatcgatag gcgcaagcac ggaagattcg ccccgttaag
    13861 gcagaaaacg tctgcagaaa gcgaagccgt gtgggaggtg ttcgatgata caagggattc
    13921 aatggttaaa gaaatggaag atcgcgatgg gctcgctctt tcttttcacg gcgatagcac
    13981 tcgcgactat aggcggtgac ccgttcgagc cggaagtcag cgatttttcg caactcgctt
    14041 ggaccgaagc cttttctctc cttcaccaac ggttggagga ggagtacgcg ttcaccgagt
    14101 ggaagggcat tcaatggaat acgctttggg agaggtctta cgcaagaatc gagcgcgcgc
    14161 aagaaaaagg ggactttgaa agctactacc tcgccctgcg agagtacctg cacgttattc
    14221 cggacgggca cgtcagctgc accaacctga tcgaaatcga cgaaaagcac gtgggcggag
    14281 ggttcggttt ttcaattgcg aaggtggccg atgggcagct cattatcacg tgggtcgacg
    14341 cgaaaagcgc cgcttgggag gcgggaattc ggcctagagc cgaaatcctc gaatggaacg
    14401 gcgagagcgc gaaacccgcg ttcgatagag tttcgaccgc ctttgcgggc aactccgcca
    14461 cagacaaaga cttggacctg aagaggactt ggtatttcgt tcgcgccccc gttggcgctg
    14521 ttgtcacgat ggccttcgcc aatgaagccg acgctgaacc aaaaaccgtt tgtcttcgcg
    14581 cctatgaaga cgaaggggtg accttcaaaa gaagttaccc aagcgccatc ctgtcggacg
    14641 gtgtgcgcga tgccttcatc gacaaaccaa atcccgaccc gcttccggaa tcgatggtgg
    14701 aaacggccct cctcgaagac a
//
LOCUS       DAOPXN010000051        14689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4178_length_14689_cov_4.768074, whole genome shotgun sequence.
ACCESSION   DAOPXN010000051 DAOPXN010000000
VERSION     DAOPXN010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4178_length_14689_cov_4.768074"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..1102
                     /locus_tag="PKM49_04310"
     CDS             272..1102
                     /locus_tag="PKM49_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY82070.1"
                     /translation="MKEARLLEEMGLSQNEAKCFIALLKKSPMTGYEVAKTANVTRTM
                     VYDVLKRLERKKCVQVIEGSPKRYAAVDYKDFVRAAREECSGKLDALEKELESLTSAS
                     HRENFVFNLSGKADMISAFKKAIDQTREVIYLSTWAQEARLIAEQLKAAHQRGVKLYI
                     FSFCKLPFDFGVQYTYDLEDAHRKFPNRRITGVFDRQLLIMGEGNESIEEIGISTENP
                     MLMEMAIDQMLMDLILHRTLSHFGYLEGVSRVSDYTCEVDRFFTDIAMYTDIPKRLDE
                     "
     gene            1127..2212
                     /locus_tag="PKM49_04315"
     CDS             1127..2212
                     /locus_tag="PKM49_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82071.1"
                     /translation="MFKALSEYKPDLSKTLTTLSKTYDSAYNRKGGHVTFRALPPSDV
                     ALYSEFDLTAFDYETDIDAYANALCEMYAASFDARTRIDDNMIPAVTPLLGIGDYSAF
                     VAGEIHFQRDTSWSKPVLNSLRDVKTLPAIGSSPWYGRFLRITEALLIRLRESGIPFT
                     RGFFSPLDLAAALRGEAIYTDFYEDRDGLSELLDFCATATIRFAEDIYSLVDRELGHT
                     PYGFWYLSGNINMSEDIACMISGKLYRTLCAPHTQRVIDHFGRGHMHSHSRAMYLVKE
                     ICSLRHVVNLWLATDPNQPRPIEHLERLVADADGVCLAIDCDSFQEIEANADILKRGN
                     FSICLPVKDTREGELLADRFNRLFEDA"
     gene            2224..4545
                     /locus_tag="PKM49_04320"
     CDS             2224..4545
                     /locus_tag="PKM49_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY82072.1"
                     /translation="MIDTNKVWTLSLSVFLACIAFLWLPSVAFASEPVRVWFSHIEAE
                     NETLRAIAREFTSETGIPVEIISRRSVFDAPADLVNNAELSDRPDVIFMQAPDIGGLV
                     ASGYLLPLKIGQDLRGRFAEVAFTAFSYQGNVYGIGYSVDTSGLVYNKALIAPSDLPK
                     TWEDFFETACALTKTNAEKKVIQYGARLNPKDMWFNYPIIREYGGYYYGTLPNGSYNA
                     YDIGLDCQSMLGYVERMKCAMNENLTISNPNHTESHISHDFACGKVAMILYGLWNAQI
                     YQNMGVDYGIAPLPRHRDGSVSRPLSTVQGFVINRFSLQPEAAWAFLNYMSRDENQQR
                     LIESGNRGPQKTGERNPANIAVMSSTYIQSDEILSSLCQIGKQSEPFPNIPEGPIWYN
                     YTPTAFRTIFFGDQKEREVDPEQHLCMLADRIRADVAAMNERADPLLLPDWLWVWVLC
                     AFSGGVGLWLFIRRKRPRSRYRERPSKKETLLAWLLLTPLILLVAFFYLYPIGHNVYL
                     SLTDYSGINLRDYRVVGLTNYRYLFTAQIAGLVSLTLWTIGFAVSVIGLSFLLATLFA
                     TLLERIRFSLSRVYRVIFILPWVIPAVITLLMWQGLLETEGGLVNQLLGAFGLPAVPW
                     LSHPIWARVSVVLVMTWFSFPYFMVIVQGLLKTIPQSFYDVARIEGANAYRVFTGITL
                     PWVFRAMLPMLIMGLVMQFNQFGVYLLTQGGPAGDTLGAPGATDLLITYVFNTAFNTK
                     RYALAASYSVIIFLFVGGLALGTMILRKAATRA"
     gene            4574..5404
                     /locus_tag="PKM49_04325"
     CDS             4574..5404
                     /locus_tag="PKM49_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82073.1"
                     /translation="MKRKERLRAGVIHFLLILLAIVTLTPFVHIALIAFGENVVGTGV
                     AVPRSFTLRHFSLLLSETHFLRWMLNSFLIAVATVGVAVVLVSISVYALSRLRFYGRE
                     HLFRAILLIQVFPLTLAMVSIFRIFAAMGLLNRMAGLIVVNAVLASTGLILVAKGYFD
                     TIPMDIDEAAKIDGASRLTILWKINLPLAKPMMMIVAIQSFVIAYNEYVLSSTVMTQG
                     LDRMPLAVGLQSMIVGQYGTNWSLYCAGAIIGSAPMLIIFYSLQKYFIGGLTEGSVKS
                     "
     gene            5302..7098
                     /locus_tag="PKM49_04330"
     CDS             5302..7098
                     /locus_tag="PKM49_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNY82074.1"
                     /translation="MRGRDHRKRADAHHLLQLAKILHRRTHGRECEIINRSAVFHLPE
                     HVYAYPTRPDTLVVRLKTARRDIRKAVVLYKNIYDHHSLPYEKDMALVGRSEDSDWFE
                     TEIALSEKRFKYYFKLDDGREVTYFCASGFLNDPEETDSFFYPYINGCDLLDLPEWAR
                     GEIIYQVWIDRFFDGDPRNNPPEVKPFDALPDRNTYYGGDFAGIIKKLDYLAEMGVRI
                     LYLSPLFLSGSYHKYDVIDYTKVESIYGGEEGLVALVQAAHAKGMRIVLDAVFNHCSD
                     QHVFFQDVLRNQSQSQYAEWFTIHRFPIEDRTRDYDSFGGLIPSMPRFNTDHPDVIDY
                     LVGIAEDWTKRLGIDGWRLDVADEVSHALWKAFRRRLRRVKNDLLIIGEIWNQAGRWL
                     LGDEMDTVTNYPFMKWLRNFARGEIDANRFWQRMSANAMLYKTPIHDYLVNLVGSHDT
                     VRNRRFVGDETIHELMLMTMLCFSGMPLIYYGDERAMDGGEDPDNRRAMQWDYNPSFR
                     DRIAALGRIRAQSDVLKKGKLIPFALSDRALAFARVLRDKRFLAVANFDTLPTNRIQV
                     DRKPTLLFGRAQTEWNGVIVPGRSFALFEEHT"
     gene            7134..8270
                     /locus_tag="PKM49_04335"
     CDS             7134..8270
                     /locus_tag="PKM49_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82075.1"
                     /translation="MTHYRWFKAMIVIVLLVNAVFMFAASPRYFLGTSANGYQVPKDG
                     GLELMPIPGRDGWYTITIDFNEDNRDPMYDGHYYKVTDGTWSASGSWGTDHYAFQPAP
                     VMITPDGQVAGLGSIYIKENTVLTILFDSNTKTIYDNAIQVFPTPRIYGSFNSAMGRG
                     SDWSMKDGEALELADIYGDGTYHGFYTLPAFTGEGDGYMMATVLSTRFEPAWTIFGAY
                     EQYVFDGTAGGMGKVSYLKPAEETTYVFTFDPKTKVTEVSPVFAGEIVALPGPTVYGD
                     FNGWVVFGENALVFQKTEDVGKYRLTLTLPAYKGEGEGYMILVALSKKFYDDQWGKRW
                     GVEEQYKLDGAPAGFGQASFLKPDRETVYTLTYDAATHVTSVSQ"
     gene            8330..9385
                     /locus_tag="PKM49_04340"
     CDS             8330..9385
                     /locus_tag="PKM49_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY82076.1"
                     /translation="MMFRRADREPDFENLRLVLQKKAPPRAVLFEFLIGEHKEKKLTG
                     AEYRTDTEFDRVVTTIKAFDSGGYDFAPIIVRGLSFERKTHFVSHAQTKSLNEGSVIV
                     DRRSFENYTWPEIANCDFSIITEAAAYLHPKAKLIPFSHDGILENVIGIVGYERLCLM
                     LYEDKTLVSEIFAEVGKRILSYFQACLPYPEVGAVILNDDWGFNTHTLISPRDLRQHV
                     FPWYKRIVAMAHDYGKCAILHSCGKYDAILEDIFDMGFDARHSYEDGITPVEKAYDAL
                     KGRLAVLGGIDVGFLARESAEAVYERSTRMLRRAAALGGYALGSGNSVPDYIPDENYL
                     AMLQAAFDWEKQEGGRV"
     gene            9382..10029
                     /locus_tag="PKM49_04345"
     CDS             9382..10029
                     /locus_tag="PKM49_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HNY82077.1"
                     /translation="MTLLEELKNAIINVEPKKAEETTHLLLNQGVGAADILNQALIPG
                     MDEVGRLFKEGEYYVPEVLVAAKAMNATLNLIEPLLIQGDVPRRGKIVIGTVEGDLHD
                     IGKNLVSMMWKGAGYTIVDLGVDVTPERFVEAVRQEKPDYLAISALLTSTMLHIPAVI
                     QALQAAGLRDDVKVLVGGAPLSQPFANEIGADGYAADATDAVSLVRRIDLEETGT"
     gene            10035..11420
                     /locus_tag="PKM49_04350"
     CDS             10035..11420
                     /locus_tag="PKM49_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY82078.1"
                     /translation="MKEVLIQTLQGKKAERTPWVPFVGVHGGYLIGENARSYLRSDET
                     LYKGLREAIIRYQPDGIPIVFDLQLEAEILGCDLRWAADGPPCVISHPLENRSFERLP
                     LPTARSGRIPLIMKTVDRLKAKHPEVALLGLITGPLTLATHLKGTELLTQMILEPDAV
                     EVVIAFCIEVAEIMASLYAEKGVELIAVVDPMISQIAPRTFKRFLLDPYQRLFERIRS
                     MGSLSASFVCGDARKNLSVISETKPDSLFVDENVPLAQLRDCAKAAGISFGGNIPLAQ
                     VLFSGSPEKCQLSAAKCIYESGGRGHILAPGCDLLYATPERNLAAIGEMVHAVSYRET
                     GALIASLSAKLPQERMEPYELPDYQNRTTLTIDIITLNSRSCAACQYMVEAVSALIED
                     FPNLPIVWREHSLMEKQTLPMIKAFDVKGIPAIVIDGEVTFESVTPAREELLERIKRA
                     LKRHPRIPPQF"
     gene            11435..13279
                     /locus_tag="PKM49_04355"
     CDS             11435..13279
                     /locus_tag="PKM49_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNY82079.1"
                     /translation="MDKTFDVLYKTRLFKTSSEETLYETLRRHGVYVQSACGGTGRCG
                     KCRIRVQGTLSEPDDTEKSILGEGELERGIRLACRAKARGDLRILEIDSLESDAFALS
                     EPFSASERQRIEGCESRIQLLERTWPEETEAASDMEWLFQDLGQEAPRSSLFIEDLSP
                     VLAASLSRKLPDLLRFPRFTEIRLDGQLRGFLTEAEALNPHYYIAVDIGTTTLTLFLL
                     DTNGKWKGGLTAKNPQIPFGTDVIARINHARTPGGLEELRTRLTTKIDGMIQEVKDTQ
                     RISAERILCVGLVGNTCMQQLFWGILPERLGERPFRAFTTETLSSNAIEAGFYALEAH
                     TPVVFLPSVAGYVGSDALAGALWCELDRQQSPYLLVDIGTNGEMMLNRGNEVFCCSTA
                     AGPALEGMNISHGMPGVEGAITGVDTAENGEIRYTVIGDGPPKGLCGSGLISLIAWLL
                     QQRVLKPGGAFEKPESLANPPKDLWRDERGQVRWRIRGDEVFLDQRDVRQFQLAKGAI
                     RCGIDILLENAGMRPEDLAGVFLSGSFGNALDRRAAVATGLLPPIDPSKITCVGNAAC
                     KGLSLWLACRQSTERLENLRKRMRSVRLEGHPEFSDLFGRSMRLGEFT"
     gene            13276..14016
                     /locus_tag="PKM49_04360"
     CDS             13276..14016
                     /locus_tag="PKM49_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82080.1"
                     /translation="MSVKRLTLTVPFPSVERVERVLGLSSDSKRKISKEIETLWPTLL
                     SIETNPLALFTVFRIQKAEAQGESVLSTDFEIVRGLWLEPRLPFPFNPEYAKAYYSLL
                     IGLITLGSGLEDAASRAFAEGESLKGWLADSLGTLMLRDAFSVFLERMSGEIEGELGP
                     RYAPGCPPLSLGAQKRIFELLGAAQAGMRLTKGSMIHPVKSTTFVLGIGERRTAREPV
                     PCRNCEHAALCYGGVFEENPKGVGGVMR"
     gene            complement(14006..>14689)
                     /locus_tag="PKM49_04365"
     CDS             complement(14006..>14689)
                     /locus_tag="PKM49_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNY82081.1"
                     /translation="DEIISAADGHSTLFQMLEGKYLTKTLQNAYTRFPLFPSSVFIAL
                     GIARDMKDEPKLLSLELDSPLELPDKSMHPFLSVTLRHYDPTLAPAGKTSASVLLNTW
                     NGEFWKDLAGKDRPAYNEMKKEIGNQVIGLLEKRFPGIRSDIEEIDVSTPYTVQRYTR
                     NWRGSYEGFGLTPETFMTRFPKMLPGLSHFSMIGQWTTPGGGLPPAALDGRNIAAKIC
                     RKDGRPFIA"
BASE COUNT         3291 a         3882 c         4229 g         3287 t
ORIGIN      
        1 cgatagtctg taagacttca gggggaagat aggtctctaa gaacgcttgg gtattcgcga
       61 cattccccag tacttctttg aaaaacctat cgtgggggtt ataaaacttc tccatccttg
      121 tcctccctac gggaaatcat ttcacgggta ttatacacta ataaagaggg agagtgtggt
      181 tttcgtgaga aaaaaggctt gacagagccc aatccatatg atagaatagt tgtaaggact
      241 atcactacta ctcttagttc ggagggatcc gatgaaagag gcgcgtttac tcgaagagat
      301 gggattgtca cagaatgaag ccaaatgttt catcgcgcta ctcaagaaaa gcccgatgac
      361 cgggtatgag gtggcgaaga cggccaatgt gacacgaacg atggtctatg atgttctgaa
      421 acgcctcgaa cgcaagaagt gcgtgcaggt gatcgagggc agcccaaagc gctacgctgc
      481 cgttgactac aaagacttcg tccgcgcggc gagagaggag tgttcgggaa aactcgacgc
      541 gttagaaaaa gagttagaat cgttaacaag cgcctcacat cgcgaaaact tcgtctttaa
      601 cctttccgga aaagcggata tgatttctgc ttttaagaaa gcgatcgatc aaacccgaga
      661 agtgatctat ctgtccacct gggcacaaga agcccgattg atcgcggagc aattgaaagc
      721 ggcccatcaa cgcggcgtga agttatacat cttttccttc tgcaagctcc cgttcgattt
      781 cggtgtacag tacacctacg atctggaaga cgcccaccgt aaattcccga atcgacgtat
      841 cacgggggtt tttgatcgtc aactcctgat tatgggcgaa ggcaacgaga gcatcgaaga
      901 gatcgggatt tccaccgaga accctatgct tatggaaatg gccatcgatc agatgctgat
      961 ggatttgatc ctacaccgta cgctcagcca cttcggctac ttggaagggg tatctcgcgt
     1021 cagcgactac acctgcgaag tcgaccgttt ttttacggat attgcgatgt acacggatat
     1081 cccgaaacgg ttggatgaat aaccgacgcc gttaaggagg aagaggatgt ttaaagcgct
     1141 ctctgagtac aaaccagatc tgtcgaaaac ccttacaacc ctgtcgaaga cctacgatag
     1201 cgcctacaat cgtaaaggag gtcacgtgac cttccgcgcg ctgcctcctt cggatgttgc
     1261 gctatactcg gagttcgacc tgaccgcctt cgactatgaa acggatattg atgcctacgc
     1321 gaacgcgtta tgtgaaatgt acgccgcttc tttcgacgcg agaacccgaa tcgatgacaa
     1381 tatgattccg gccgtcacgc cccttttagg gataggcgac tattccgctt tcgtcgcggg
     1441 agaaatccat ttccaaagag acacgagctg gtcaaaaccc gtactgaaca gccttcgaga
     1501 tgttaaaact ttgcccgcga tcggctcgag tccgtggtac ggacgtttcc ttcgaattac
     1561 ggaagcattg ctcatccgtc tccgagaaag cggaatacct ttcacgcgcg gattcttctc
     1621 gccgcttgat ttagccgccg cgctacgtgg ggaagccata tacacggact tttacgagga
     1681 ccgtgacggc ctttccgagc tgctggactt ctgcgcgacc gcgacgatcc gcttcgccga
     1741 agatatctac tccttagtag accgcgaatt ggggcacacc ccttacgggt tctggtacct
     1801 gagcgggaac atcaatatgt cggaagacat tgcctgcatg atctccggta aactgtatcg
     1861 aaccctctgc gctcctcata cccagagggt gatcgatcat ttcggaagag ggcatatgca
     1921 cagtcattcc cgagcgatgt acctggttaa agagatctgt tccctgcgcc acgtggtgaa
     1981 cctgtggctc gctaccgacc caaaccagcc ccgccccatc gagcatctcg agcgtttggt
     2041 ggccgacgcg gacggcgttt gtctggcgat cgattgcgac tctttccagg agatcgaagc
     2101 gaacgcggac atcctgaaaa gagggaattt ttctatctgc cttcccgtga aagacacgcg
     2161 ggagggcgag cttctggcag accggttcaa ccgtcttttc gaggacgcct gagagaagcg
     2221 ggggtgatcg atacgaacaa ggtttggacg ctttctcttt ccgtttttct tgcgtgtatt
     2281 gcgtttttat ggctcccttc cgtcgccttc gcctccgaac cggtgcgggt atggtttagc
     2341 catatcgaag cggaaaacga gaccttgcgg gcgatcgccc gggagttcac ttctgaaacc
     2401 gggattccgg tggaaatcat cagtcgtcga agcgttttcg acgctcccgc cgatcttgtc
     2461 aacaacgccg agctgtcgga tagaccggac gtgatcttca tgcaggcgcc cgatatcggg
     2521 ggactcgtcg cctcggggta tctgctcccc ctgaagatcg gccaagacct ccgcgggcgg
     2581 ttcgccgagg tggctttcac cgccttctcc tatcaaggga acgtctacgg gatcggttac
     2641 tccgtggaca catcggggct agtctacaac aaagcgctaa tcgcaccgag cgaccttccg
     2701 aagacgtggg aggacttttt cgaaaccgcg tgcgccctca ccaaaacgaa cgcggagaaa
     2761 aaggtgatcc aatacggcgc gcgcctgaac cccaaggata tgtggttcaa ttatccgatc
     2821 atccgtgaat acggaggata ctactacggg accttgccaa acggctccta caacgcttac
     2881 gacattgggc tagactgcca atctatgctg gggtatgttg agcggatgaa gtgcgcgatg
     2941 aacgaaaacc tgaccatttc gaaccctaac cacaccgaaa gtcacatctc ccacgacttc
     3001 gcctgtggaa aggtcgccat gatcctatac ggtctatgga atgctcagat ctatcagaat
     3061 atgggcgtcg actacggaat cgcgccgctc ccccgacacc gtgacggttc tgtttcccgg
     3121 cccttgtcca cggtgcaggg attcgtgatc aaccgtttct cacttcaacc ggaggcagcg
     3181 tgggcgtttt tgaactatat gtcgcgggat gagaaccaac agcgtttgat cgagtccgga
     3241 aacagaggcc cgcaaaaaac cggagaacgc aaccccgcca acatcgcggt gatgtcgagc
     3301 acctatatcc aatccgatga gatactctcc tccttatgtc agatcggtaa acagagcgag
     3361 cctttcccga acattccgga aggcccaatc tggtacaact acaccccaac cgccttccgt
     3421 acgatcttct tcggagacca aaaggagcgg gaagtcgatc cggagcaaca tctgtgcatg
     3481 ctggctgaca gaatccgcgc ggatgtggcg gccatgaacg aaagggccga tcccctactg
     3541 cttcccgact ggctttgggt ttgggttctc tgcgccttct cgggcggagt tggcctctgg
     3601 ctttttattc gacggaaaag gcctcgatcg cggtatcgtg aacgaccgag caagaaagag
     3661 accctgctcg cctggttgtt gctgaccccg ttgatccttt tggtggcctt cttctatctc
     3721 tatccaatcg ggcacaacgt ctacctttca ctcaccgatt acagcgggat caacctgcgc
     3781 gattatcgcg tggttggtct gaccaactac cgctaccttt tcacggcgca aatcgcgggg
     3841 cttgtctcat taacgctctg gacgataggc ttcgcagtgt cggtgatcgg cctttcgttc
     3901 ctgctcgcca cccttttcgc cacgctcctc gaacggatcc gtttttctct ttcgagggtt
     3961 taccgggtga tcttcatcct gccctgggtg atcccggcgg tcatcaccct gctgatgtgg
     4021 caggggctgc tcgaaaccga gggcggattg gtcaaccagc tgcttggcgc tttcggtcta
     4081 ccggccgttc cctggctctc tcatcccata tgggcgaggg tcagcgtcgt gttggtgatg
     4141 acttggttct ctttcccgta cttcatggtg atcgtccagg gactattgaa aacgatccct
     4201 cagagcttct acgatgtggc gcggatcgaa ggggccaatg cctaccgtgt attcaccggg
     4261 atcacccttc cctgggtgtt tcgggcgatg ctacccatgc tgatcatggg tttagttatg
     4321 caattcaacc agttcggcgt ttatctgttg acacaagggg gaccggcggg cgacacactt
     4381 ggtgccccgg gcgccaccga tctattgatc acctatgtct tcaacaccgc cttcaatacg
     4441 aaacggtacg cgttggccgc ctcctattcc gtcatcatct ttctcttcgt gggcgggttg
     4501 gcgttgggaa cgatgatctt gaggaaggcc gctacgcgcg cctgacggaa aaactcgtgg
     4561 agggaggttg gcgatgaagc ggaaagagag gctaagggcg ggggttattc actttcttct
     4621 aatcctgtta gccattgtga cgctgacgcc gttcgtgcac atcgcgttga tcgctttcgg
     4681 tgaaaacgtg gttggaaccg gagtcgcggt ccctcgctcc ttcacgttgc gtcatttttc
     4741 ccttttgctg agcgaaaccc attttctcag atggatgctc aattcctttc tcattgcggt
     4801 agcgaccgtg ggggtcgccg tcgtgctggt gtctatctcc gtctatgccc tttctcgcct
     4861 gcggttttac ggacgcgaac acctctttcg cgccatccta ctgatccaag tcttcccgct
     4921 taccctggcg atggtttcga tctttcggat ttttgccgcg atgggacttt tgaatcgtat
     4981 ggccgggttg atcgtcgtga atgcggtgtt ggcttccacc gggttgatcc tcgttgccaa
     5041 agggtacttc gataccatcc cgatggacat cgatgaagcg gccaaaatcg acggggcttc
     5101 ccggttgacc atactgtgga aaatcaacct tccgcttgcc aagccgatga tgatgatcgt
     5161 ggccattcaa agcttcgtca tcgcgtataa cgaatacgtg ctttccagca cggtgatgac
     5221 gcagggcctg gatcgtatgc cgttggcggt ggggcttcag agcatgatcg tggggcagta
     5281 tggaaccaat tggagcctgt attgcgcggg cgcgatcatc ggaagcgcgc cgatgctcat
     5341 catcttctac agcttgcaaa aatacttcat cggaggactc acggaaggga gtgtgaaatc
     5401 ataaaccgaa gcgcggtctt tcatcttccc gagcacgtat acgcctatcc gacgcgtccc
     5461 gacaccctgg ttgtccggct caagacagcc cgaagagata tccgaaaggc tgtggtgcta
     5521 tacaaaaaca tctacgatca ccattccctt ccctatgaaa aggacatggc gctcgtgggc
     5581 cgaagcgagg atagcgattg gtttgaaacg gagatcgccc tcagtgaaaa acggttcaaa
     5641 tactacttca aattggatga tgggcgggag gtcacgtatt tttgcgcgtc ggggtttttg
     5701 aatgatcctg aggagaccga ctcctttttc tatccctaca tcaacggatg cgacctcttg
     5761 gatcttccag aatgggctcg aggggagatc atctaccagg tgtggatcga ccgttttttc
     5821 gacggtgatc ctcgaaacaa cccccccgaa gtgaagccct tcgacgcctt accggacaga
     5881 aacacctatt acgggggaga tttcgctgga atcataaaaa aactcgacta cctggcggag
     5941 atgggggtca gaatcctcta tctcagcccg ctatttctat ccggaagcta ccacaagtac
     6001 gatgtgatcg attacacgaa agtggagtcc atttacggtg gagaagaggg gctcgtggct
     6061 ctcgttcaag ccgcgcacgc caaagggatg cggatcgttt tagacgcggt ttttaatcat
     6121 tgcagcgatc aacacgtatt cttccaggat gttttaagga accaaagcca atctcagtat
     6181 gcggagtggt tcacgatcca tcgttttccc atagaggacc ggacacgaga ctacgacagc
     6241 tttggcggat tgatcccctc catgccccgc ttcaatacgg atcaccctga tgtgatcgac
     6301 tatcttgtcg ggatcgccga agactggacg aaaaggctgg ggatcgacgg ttggcgattg
     6361 gatgtggcgg acgaagtctc ccatgccctt tggaaagcct ttcgccgtag gcttcggcgt
     6421 gtgaagaacg acctactgat catcggcgaa atctggaatc aggccggccg ttggctgttg
     6481 ggcgacgaga tggatacggt gaccaactac ccgtttatga agtggttgcg gaacttcgcg
     6541 cgcggagaga tcgatgcgaa ccgtttttgg cagaggatga gcgcgaacgc gatgctctac
     6601 aaaactccga ttcacgatta tctcgtcaat ctcgtcggca gccacgacac cgtgcggaac
     6661 cgccgtttcg tcggggatga gacgatccac gagctgatgc tgatgacgat gttgtgtttt
     6721 tccgggatgc ctttgatcta ttacggcgac gaaagggcga tggacggggg tgaggacccc
     6781 gacaaccgac gggcgatgca gtgggactat aacccatcct tccgtgacag aatcgcggcc
     6841 cttgggagaa tacgcgctca atccgacgtg ttgaagaaag ggaaactgat tccgttcgcc
     6901 ttatccgacc gggcgctggc tttcgcgcga gtcctgcgcg ataagcgctt cctggctgtg
     6961 gccaacttcg acacgcttcc gacgaaccgg atacaggtgg acaggaaacc gacgcttctc
     7021 tttgggcggg cgcaaaccga atggaacggt gtgattgtgc ctgggcggtc tttcgccttg
     7081 tttgaagaac atacgtaatc tttttcgcct acaataaaag ggagggaaga acaatgacgc
     7141 attatcgatg gtttaaagca atgattgtga tcgtgttgtt ggtaaacgcc gtgtttatgt
     7201 tcgctgcgag tccgcgatat tttctcggga ccagcgcaaa cgggtatcag gtaccaaaag
     7261 atggagggtt ggagttaatg ccgatccccg gacgagacgg atggtatact atcacgattg
     7321 acttcaatga ggacaatcga gaccctatgt atgacgggca ctattacaag gttaccgatg
     7381 gcacgtggag tgcgagcgga tcctggggaa cggaccacta tgccttccag cccgctccgg
     7441 ttatgatcac acccgacggt caggtggccg ggctgggctc tatttacatc aaagagaaca
     7501 ccgtgctcac gatccttttc gactcgaata ccaaaacgat ctatgacaac gcgatccagg
     7561 tgtttcccac gccgcgtata tacggcagtt ttaacagcgc gatgggcaga ggctccgatt
     7621 ggagtatgaa agacggagag gcgctcgaat tggccgatat ctacggggac gggacctatc
     7681 acgggttcta taccctccca gccttcacag gggaaggcga cggatacatg atggcgacgg
     7741 tgctttccac acggttcgaa cccgcttgga ccattttcgg cgcttacgag caatacgtgt
     7801 tcgacgggac ggcgggggga atggggaagg tcagttacct gaaaccggcg gaagaaacca
     7861 cctacgtgtt cacgtttgat ccaaaaacga aagtcacgga agtcagcccc gttttcgcag
     7921 gagagatcgt cgcgcttccg gggccgacgg tgtacgggga cttcaacggg tgggttgtct
     7981 tcggagaaaa cgccctcgtc tttcaaaaga ccgaagatgt gggaaagtac cgccttacct
     8041 tgaccttgcc cgcgtataag ggtgagggag aggggtatat gatcctcgtc gccttatcca
     8101 aaaagtttta cgacgatcaa tgggggaaac gatggggtgt ggaggagcag tacaagctgg
     8161 acggcgctcc agcggggttc ggtcaagcca gtttcctaaa acccgatcgg gagaccgtct
     8221 acacgttaac ctatgacgcc gctacacacg tgacgagcgt atcccaataa ccagggcgaa
     8281 ccggttgcgg aaggctttcg cgaccggtta ccctttcgaa ggagacagaa tgatgtttcg
     8341 aagagcggat cgcgaacccg actttgaaaa cctgaggttg gttctgcaaa agaaagcgcc
     8401 cccgagggcg gtgttatttg agtttttgat cggagaacac aaagaaaaga aactgacggg
     8461 ggcggagtat cggactgaca cggagttcga ccgtgtcgtg acgacgatca aagccttcga
     8521 ctcgggggga tacgatttcg ctccgattat cgtgcgcggt ttgagtttcg agcgcaaaac
     8581 ccactttgtc agccacgcgc agacgaagtc tctgaacgaa ggaagcgtga tcgtcgatcg
     8641 caggtccttc gaaaactaca catggccgga aatagcgaac tgcgattttt ccatcatcac
     8701 cgaagccgcc gcttacctgc atccgaaagc gaagctcatc ccattttctc acgatggcat
     8761 tttggaaaac gtgatcggca tcgtcggcta cgaacgcctg tgcctgatgc tttacgaaga
     8821 caaaaccctg gtttcggaga tcttcgccga ggtggggaag aggatcctgt cctactttca
     8881 ggcctgtctg ccctatcccg aagtgggtgc cgtgatcctc aacgacgact ggggatttaa
     8941 cacccacacg ctgatctccc cgagggactt gcgccaacac gtctttccgt ggtacaagcg
     9001 tatcgtcgct atggcccacg attacggcaa gtgcgcgatc ctacactcct gcggtaagta
     9061 cgacgcgatc ctcgaggaca ttttcgatat ggggtttgac gccaggcact cttacgagga
     9121 cgggatcact ccggtggaga aggcctatga cgcgttgaaa gggcggctcg cggtccttgg
     9181 ggggatagat gtggggttcc tggctcgaga gagcgccgaa gcggtgtacg aacgatccac
     9241 gcggatgtta cgccgagcgg cggccttagg gggatatgcg ttgggaagcg gtaattccgt
     9301 tccggactat attccggatg agaactatct ggcgatgctg caagccgctt ttgattggga
     9361 aaaacaggaa ggaggacgcg tatgacgctt ctcgaagaat tgaagaacgc gattataaac
     9421 gtggaaccaa aaaaagccga agagacaacg cacctgctgc tgaatcaggg tgttggcgcc
     9481 gcagatatct tgaatcaggc gctgattccg gggatggacg aagtggggcg gctgtttaaa
     9541 gaaggagaat actatgtgcc tgaggtcctc gtggccgcca aagcgatgaa cgcgacactg
     9601 aaccttatag agccgcttct cattcagggc gatgtgccgc gtagaggcaa gatcgtgata
     9661 gggacggtcg agggagacct tcacgacatc gggaaaaact tggtgagtat gatgtggaag
     9721 ggcgccggat acacgatcgt cgacctcggg gtcgatgtca caccagaacg gttcgtggaa
     9781 gcggttcgtc aggaaaaacc cgattacctc gccatttcgg cgctattgac cagcacgatg
     9841 ctccacatcc ctgccgtgat tcaagcgctg caggcggcag ggctgcgcga tgacgtaaaa
     9901 gtgttggtag gaggggcccc cttatcacaa ccctttgcga atgagatcgg cgcggatgga
     9961 tacgccgccg atgcgacgga cgcggtgagc ttggtccgac ggatcgatct cgaggaaacg
    10021 ggaacgtgag cgcgatgaaa gaggttttga tacagactct tcagggcaaa aaagcagagc
    10081 gcaccccttg ggtcccgttt gtgggggtgc acgggggata cctgatcggc gagaacgcga
    10141 gaagctatct gcgttcggac gaaacgctat ataagggcct tcgggaggcg attattcgtt
    10201 accaacccga cggcattccg atcgtcttcg atctccagct cgaggcggag atcttgggat
    10261 gcgatctgcg atgggcggcg gacggccccc catgcgtgat ctctcaccct ttggagaaca
    10321 ggtctttcga acgcttgccc ctgccgacag cgcgcagcgg acggatcccg ctcattatga
    10381 aaacggtgga tcgtctaaaa gccaaacacc ccgaggtcgc gcttctgggg ttgatcacgg
    10441 ggccattgac gctggccact catttgaagg gcaccgagct gttgactcag atgatcctcg
    10501 aacccgacgc ggtcgaggtg gtgatcgcat tttgtataga ggttgcagag atcatggcga
    10561 gcctgtatgc ggagaaaggc gtggaattga tcgcggtcgt cgacccgatg atctcccaga
    10621 tcgccccgcg aacgttcaag cggtttttac tcgatccgta ccagcgtctg tttgaacgca
    10681 tccgaagtat gggcagcctt tccgcctcct tcgtatgcgg agacgcccga aagaaccttt
    10741 cggtcatcag cgaaaccaag ccggatagcc ttttcgtgga tgagaacgta cctctggcgc
    10801 aactcaggga ttgcgcgaag gccgcgggca tctctttcgg ggggaatatt cccctcgctc
    10861 aagtgctctt ttcgggctca ccggagaaat gccagctttc cgcggcaaag tgcatctacg
    10921 aatccggagg aaggggccac atcctcgccc ccggatgcga cctcttgtac gcgacaccgg
    10981 aacgaaacct ggcggcgatc ggagaaatgg ttcacgctgt gagctatcgc gaaacgggcg
    11041 ccctcatcgc ctctttgtcc gcaaaacttc ctcaggagcg gatggagccc tacgagctgc
    11101 cggattacca aaatcgaacg accctgacga tcgatatcat cacactaaat tccaggagct
    11161 gcgcggcctg tcaatatatg gtcgaagcgg tcagcgcgct gatcgaggat ttcccgaact
    11221 tgccgatcgt atggcgcgaa catagcctga tggaaaaaca gacgctcccg atgatcaagg
    11281 ccttcgatgt gaaaggaatc ccggcgatcg tcatcgacgg agaggtgacc ttcgaaagcg
    11341 tcacccccgc tcgcgaagag ctgctcgagc gaatcaagcg cgcgttgaaa cgccatcccc
    11401 gcataccgcc ccagttttag gtgtactatc cgttatggac aagactttcg atgtcctcta
    11461 taaaacgcgc cttttcaaaa cgagttcgga ggaaacgctt tacgaaaccc tacgaaggca
    11521 cggggtctac gtgcaatccg cttgcggtgg aaccgggcgt tgcgggaaat gcagaatccg
    11581 tgtccaaggg accctgagcg aaccggatga caccgagaaa tccatcttag gggaaggcga
    11641 gctcgaacgc ggaattcgct tggcctgccg ggcgaaagcg cgcggcgatc tgcggatttt
    11701 ggagatcgat tctctggaat cggacgcttt cgcgctctcc gagcctttca gcgcatccga
    11761 gcggcagagg atagaagggt gcgaatcgcg gattcagctg ctcgaacgaa cgtggccgga
    11821 ggaaacggag gccgcctccg atatggagtg gctttttcaa gacctcgggc aggaagcgcc
    11881 gcggtcgtcg ctttttatag aggacctctc tccggtgttg gctgcctccc tctctcggaa
    11941 gctgcccgat ctgcttcggt ttcctcgatt caccgagatc cgcttggatg gccagctcag
    12001 gggctttctc acagaggcgg aagcgctaaa cccccattac tatatcgctg tcgatattgg
    12061 cacgaccacg ttaacgctct ttctcctcga cacaaatggc aagtggaaag gcgggctcac
    12121 cgcgaagaac cctcagattc cgttcggaac cgacgtgatc gcgcggatca accatgcccg
    12181 aacgccagga ggtttggaag agctgagaac gcggctgacg accaagatcg acgggatgat
    12241 acaagaagta aaggataccc aacggatatc ggcggagcgg atcctctgcg tcgggctcgt
    12301 cggtaacacc tgcatgcagc aattgttttg ggggatactg cccgaacgtt tgggtgagcg
    12361 tccgtttcgg gcgttcacga ccgaaacatt gtcttcgaac gcgatagagg ccggtttcta
    12421 tgcgttggaa gcccatacgc cggtggtctt cttgccgtcc gtggccggtt atgtcggatc
    12481 ggacgcgttg gcgggcgccc tgtggtgtga gctcgatcga cagcaaagcc catacctctt
    12541 ggtcgacatc ggaacgaatg gagagatgat gctgaaccga gggaacgagg tgttctgttg
    12601 ctccaccgcg gcgggtccag cgctggaagg aatgaacatt tcccacggca tgcccggagt
    12661 cgaaggcgcg ataaccggcg tcgacaccgc cgaaaatggt gaaatccgat acaccgtcat
    12721 cggagacggg ccgcctaaag gcctttgcgg ttcgggactg atctctttga tcgcctggct
    12781 actccaacag cgggtattga aacccggagg cgccttcgaa aaacccgaat cgctggcgaa
    12841 tccgccgaag gacttgtggc gtgatgagcg aggtcaggtt cgctggcgta tacgcggaga
    12901 cgaggtgttc ctggatcagc gggatgtccg gcagttccaa ctcgccaaag gagcgattcg
    12961 ttgcggcata gatatattgc tggagaacgc ggggatgaga ccggaagacc tggcaggagt
    13021 ctttctttcg gggtctttcg gtaacgcgtt ggatcgtcgg gccgcggtgg cgacggggtt
    13081 gttgccaccc atcgaccctt caaaaatcac ctgtgtgggg aacgccgctt gtaaagggct
    13141 atcattgtgg ctcgcctgcc gacaatctac cgagcggttg gagaacctgc ggaaaaggat
    13201 gaggagcgtg cgtttggaag gtcatccgga gttttccgat ctctttgggc gttcaatgag
    13261 gctgggcgag ttcacgtgag cgtgaagcgg ctgactttaa cggtcccctt cccatccgtg
    13321 gagagggtag aaagggtcct tggcctctcg agcgactcca aaagaaagat atcgaaagag
    13381 atcgagacgc tctggcccac gttgttatcg atagaaacga acccgctcgc cctgtttacg
    13441 gtctttcgca tccaaaaagc ggaggctcaa ggggagtcgg ttctttctac cgatttcgag
    13501 atcgtgcgcg ggctatggct cgaaccccgg cttccgtttc ctttcaaccc ggagtatgcg
    13561 aaggcctact attccctcct gatcggcttg atcaccttag ggtcggggtt ggaagacgcc
    13621 gcttcgcggg cgtttgcaga gggagagtcg ctcaaaggat ggttagccga cagcctcggc
    13681 accctgatgc tccgagatgc cttttcggtc tttctggaac gaatgagcgg cgagatcgaa
    13741 ggggaactcg ggccacgata tgcgcccggt tgccctcccc tctcgcttgg cgcgcagaag
    13801 cgcatcttcg agcttctcgg ggccgctcaa gcaggaatgc gcttgacgaa gggatcgatg
    13861 atccatccgg tgaaatccac gaccttcgtc ttgggcatcg gagagcgccg gacagcgcgc
    13921 gagcccgttc cctgtagaaa ttgcgaacac gccgctcttt gttatggcgg cgtgttcgaa
    13981 gaaaacccca aaggcgtggg cggggttatg cgataaaagg ccgcccgtct tttctgcaga
    14041 ttttggccgc gatattcctg ccgtcgagcg cggcgggtgg caggccgcca ccgggggtcg
    14101 tccactggcc gatcatagaa aaatgggaca aacccggaag cattttcggg aagcgtgtca
    14161 taaaagtttc gggagtcagt ccgaaccctt cgtagcttcc gcgccaattg cgcgtatacc
    14221 gttggaccgt atagggtgtg gagacgtcta tttcctctat atcagagcgt attcccggaa
    14281 agcgtttttc caaaaggcca atcacttggt ttccgatttc ctttttcatc tcattgtacg
    14341 cgggtcggtc tttgccggcg aggtctttcc agaattcgcc gttccaagtg ttcaataaaa
    14401 cggaggcgga ggtctttccg gcaggcgcga gtgtcggatc gtaatggcgt agggtcaccg
    14461 agaggaatgg gtgcatgctc ttatccggca gttcgagagg cgaatccagc tctagagaaa
    14521 gcagtttcgg ctcgtctttc atatcccggg cgattccgag cgcgatgaaa accgaggaag
    14581 gaaagagagg gaagcgtgtg taggcgttct gcagcgtttt tgtgaggtat ttcccctcca
    14641 gcatttgaaa cagcgtggag tgcccatcag ctgcggagat gatttcgtc
//
LOCUS       DAOPXN010000052        13859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4507_length_13859_cov_5.229281, whole genome shotgun sequence.
ACCESSION   DAOPXN010000052 DAOPXN010000000
VERSION     DAOPXN010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_13859_cov_5.229281"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..1075)
                     /gene="flgA"
                     /locus_tag="PKM49_04370"
     CDS             complement(32..1075)
                     /gene="flgA"
                     /locus_tag="PKM49_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HNY82082.1"
                     /translation="MIPPSSHNRCRFCGFLLLLVLWAGLFASDVKPLILLPYDQSRIY
                     FADFFDIESIPSPELSEKLRTIVFGFAPLPGKTLVFQSDYLRAKIALYFPEIEPIIRS
                     ETITVSRGLTPTSSSWGAAPPLTGATETVDIPAETFLSPNEWKSRLETLTLAFLSETW
                     SVAIPPEAFHLELAIEPPSVFSDRPWDLTFTKFGKSEYFVKIVADSPVNSPATRWTGK
                     VKPIWIRNLATAGRNISYKESFSSDAVVFKPLNYFDYRDPVVEGVSFPKVAKAFIKSG
                     AVIEGTMIANPPYVKAGQVIFAVVRIGGVEVKGLVEALKDAQIGEVIQARNVETGALL
                     TGIVEEGPVLRLH"
     gene            complement(1072..1398)
                     /gene="fliE"
                     /locus_tag="PKM49_04375"
     CDS             complement(1072..1398)
                     /gene="fliE"
                     /locus_tag="PKM49_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNY82083.1"
                     /translation="MAQSMDPIGKIGSANPIQRVSRVGQTPRQAEKSEIEPDFMKILQ
                     QALEETNADQYEAERAADEFAAGRLSNIHDVLISAEKAAMSLRLTLEVRNKIVDAYRE
                     IMRMQL"
     gene            complement(1401..1853)
                     /gene="flgC"
                     /locus_tag="PKM49_04380"
     CDS             complement(1401..1853)
                     /gene="flgC"
                     /locus_tag="PKM49_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNY82084.1"
                     /translation="MLDVFRTLNISASGLTAQRARLDVIASNIANAETTRSDEGGPYR
                     RKTVVFREVYESAIHDAGYRNVRAQTDAMRGVEIAAIEADKRPFRWIYDPSHPDANEE
                     GYVQMPNVNVLREMVDLINAQRSYEANVTALNATKSFVNAALQIGKGG"
     gene            complement(1853..2260)
                     /gene="flgB"
                     /locus_tag="PKM49_04385"
     CDS             complement(1853..2260)
                     /gene="flgB"
                     /locus_tag="PKM49_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNY82085.1"
                     /translation="MAGLYNTNFTTLPLAMDVASLRNEVYAHNIANAETPGYKRREVS
                     FEDSFKKALAGDELRLKTSRVRHIPNYPVSLDGVEPDLTRIKNTVITNDGNNVDIDKE
                     MAALSANTLRYQTLTRLMDLNIVRYNIVLKGTR"
     gene            2480..3013
                     /locus_tag="PKM49_04390"
     CDS             2480..3013
                     /locus_tag="PKM49_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNY82086.1"
                     /translation="MTYGEKLRKLRMALDLTQEEVAIRADLARSFISQVETDKTSPTL
                     DNLERILKAVGSTLQAFFSEEEEEKVIYRKSEEVPLYDQPVGVQTTLLMNEVENKKID
                     ALLVTLKPGSKTDEEDYHSGDEFGYVLAGTIHLILDGVDYQCEKGDSFYYRADKKHSV
                     MNQDPTVKAKFLWIKID"
     gene            3038..3439
                     /gene="speD"
                     /locus_tag="PKM49_04395"
     CDS             3038..3439
                     /gene="speD"
                     /locus_tag="PKM49_04395"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNY82087.1"
                     /translation="MKALGKHLIAEFYDCDAETLNDVHKVEERMKSAALVAGACIVDS
                     SFHQFSPYGISGVVVISESHLSIHTWPEYGYAAVDVFTCGDHVDPWVAFDFLKKAFGA
                     QRFLIDEYQRGNYKALGISEGASYKAVEATA"
     gene            3507..4382
                     /gene="speE"
                     /locus_tag="PKM49_04400"
     CDS             3507..4382
                     /gene="speE"
                     /locus_tag="PKM49_04400"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNY82088.1"
                     /translation="MNLSEKSPFYFREVYHDKKCFLELTVLRWVHSQTTRFQKIDIFD
                     TPSHGRVFALEGIVQTMERWEFMYHEMIAHVPMMICERPKQVLVIGGGDGGTVREVLR
                     HPSVERVVLCEVDREVVEAAKTYLPTLSHAFSDERLSCVFADGAEYLKGSPQTWDVII
                     IDSTDPTAGEGGNLFTESFYALCRDRLLPKGVCVAQTENPIYDREWMRTAYRRIAGAF
                     PTAGVYLGFSPQYPSGFWTYSIGYRSEEGQSAPDFYRLSPEKMRRIPPHLRYYTADVH
                     HAAFQIPPFLSDYLA"
     gene            complement(4502..5902)
                     /locus_tag="PKM49_04405"
     CDS             complement(4502..5902)
                     /locus_tag="PKM49_04405"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HNY82089.1"
                     /translation="MRDVYFVDTTLRDAHQSLLATRMSTEEMLPILKTIDQTGYQSLE
                     VWGGATFDSCLRFLGEDPWERLRTIRKNIKRTELQMLLRGQNIVGYKNYADDVLELFV
                     KRAIKNGLDRIRTFDALNDLRNLEKSIAYTQKYKGHIQVGICFTTSPVHTLEYYSSLV
                     DRILTYEPNSICIKDMAGLLTPKISYDLVKMIKSKKDIPVEMHTHCTVGTGELSYLSA
                     FEAGADLFDTACSSMSGASSQPAVESFYYSFKEYPQTRCHLNPEHFLEISDYFETVRQ
                     KHATDDVNIKHTDLRVLSSQVPGGMLSNLYSQLKKQNSMHRLNDVMAEIPSVRKDLGY
                     PPLVTPTSQIVGVQAVLNVLTGKRYSRITKETEQYVKGHYGKPPADIDPDLKRVIVKD
                     PSEVIDCRPADLIPPQLPELRKMLGSFVEKEEELLSIALFGDLALNFLKEKYYGRIGI
                     DESIADTVENGVYPAV"
     gene            complement(6157..7338)
                     /locus_tag="PKM49_04410"
     CDS             complement(6157..7338)
                     /locus_tag="PKM49_04410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNY82090.1"
                     /translation="MDARIILKRLLSPRLANGHPWVYDNEIERIEGDITPGDTVDVFS
                     FSRQWIGRGYVNPNSKIVARILTRRNERIDRDFFFKRIAEAIDYRNAITPRSTVRRLV
                     FGEGDDLSGLIIDRLEDVFVFQFNTAGMHRFRSEIIDAVRERFPEGFFYDKSDPIALA
                     KEQVTPLNGWIGREFPEPFSVSYQGLRFSVSPSEGQKTGIYIDQLENAVIAASLCFPA
                     GKVVWDVFSNSGNFGLRMLQNGAKRVAFVDQSATAIADCRENLRLNALSGASFHEGNA
                     FDILRAWEESNEKADIIVLDPPSFTKSRSSKGNALRGYKEINLRALKALRRGGYLVSA
                     SCSQAVSRQEFEMMMYSAATDVGARLRLVYRGGQPADHPVVLNIFETDYLKFYIFEKL
                     S"
     gene            7940..8635
                     /locus_tag="PKM49_04415"
     CDS             7940..8635
                     /locus_tag="PKM49_04415"
                     /inference="COORDINATES: protein motif:HMM:NF039975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6063 family protein"
                     /protein_id="HNY82091.1"
                     /translation="MEASLREQMRWVFQMITRALVTGQISFRDDPQLYSLYFENHDIR
                     DSVRLFAQEARLLVREDTQAIYFFCAQESPFTVKKTEFFKQVSFPSKRWPLFEVITAC
                     LFLRFFEENAEYVVLSEILERLQKLKEKARPESPDRSPKKQDAAAVRWMTDWEGLPEG
                     FFRPNDWSVYKSDSQVGLIVKTVQVLKEQGLVAEYENQWTVIPTGKARAVYGMWREEG
                     AWNALGLGDTEDA"
     gene            8628..12488
                     /locus_tag="PKM49_04420"
     CDS             8628..12488
                     /locus_tag="PKM49_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82092.1"
                     /translation="MPELKGFRVLNLAYGETFVIPDLTLDFFGRNTLILMPNGTGKTY
                     LLSCFFQTVLPGAHTPSRSVEDLFSRCTGTSHILTAFSIEKETVFLGFCTRKKTDRTY
                     FNWTLRLDEPCDIDALPMRRGESVLDYDELPLLLKSLHKDRFTLFRQEARPAYEQQLE
                     RYGLGTEEWRLMRAFNLFLEGALPVQEVLPGASDRLLDPMVFPLVEKRLKMDGLDPRE
                     RVDAFFSSQNAIKPSATAGSVLALKERLQSRLSTLDDFREAKEELNNAVYSLRTIQEF
                     THKEIRKAKAELHRLGARLVALVEEEAQCDRNRQVRALQESVEKKAELERTETRLVGL
                     IEEKQQKIALLYETLRRRLTSEPDPHDSDWHTDASLESRKPVSVPWALMAKHHLESLL
                     ETVKAHQTERESALRSSWEILSIQQRCVALQERYERLGGLTRFQKIGYEKERARAALE
                     RNRRRQSIQKVLRIKLRQKQRTLEGDLERLKAQLAEVDRNAEGIQNYREFAQALTVLE
                     GARPLPLLAERLVEKRNRMCVRIAEERGEIERLDRLDAKEAEPIGARPPKEVEKLSAA
                     LERNGFSGFEKGADWIGSGKSDNPFLSFSLITEYPLEMDQALSRLPFEDFPTFPIPVL
                     SVREATNLAEASEYRTMMRLKLRWVPSLTERALQLRLSPGMRAFAEKGPVVGSDLAHW
                     LQARRASIRARIENLEGTLKKTERQLERLAYFIQAVGSDPEVARETLAEKRSLIEKDL
                     WRVSKSLGDLGEKIRASRLLSTSLDEEERALQQKIETLEREEWEWSQLSSEIDQACQV
                     PLKDFSLFCLSIQKRLAEWDAKVQEAERVIGESLEELRNPWLSLVAREGQAALTTVEE
                     QPEALWEIVARIRSLESELRELLQERAVLQARSPALLDSSLSPNLSRAIERGSQEDPL
                     SQTTETLETLGRRLREIEKERQEIAQETAEIVETLQRLETLAQRQRIFLAGKESSEKK
                     YISGLPQVFLKSDLHGLWTEKEAVCLKQLYALDRQQAALDEDWADLKKALDPTESWEE
                     PVLDSPGEEELEGGKTVVTRLLHGLKRRIDAERAEERRAQSALSCFSREIGDAMEVVN
                     AHLREIARFSKPELERAPLLEFSLPNKTREEYDKSFSSRLAEYIALQRARGQEIRLQW
                     PLRTLWEFYHDERLEIRIEKPPFTREGEGKHAWKEVEKWSTGEKMAVYLALYASLFAW
                     WKGQTPNTKPTSFLILDNLFGHINTETLLEIPMRMLYTHKFQIIGFTSSAEPFLFKYF
                     PKWIGLSPQETDHYSFFLPFHVER"
     gene            complement(12668..13441)
                     /locus_tag="PKM49_04425"
     CDS             complement(12668..13441)
                     /locus_tag="PKM49_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNY82093.1"
                     /translation="MKKTIISCICFCVFFLSFAFGLTRNGVAYVYSYTYLIPMDLSYR
                     VITPAPARVVSVVDGNTVKVSLASGTESVRLIGVATQETVHPTKTVEAFGKEASDFLK
                     KLCKDGQEVYLSYDRNPRDVNNQLLAYLWWKGDDGKWVLANLAIIENGYGYACSDFSF
                     REDYMVMFSDAQEKARENGYGLWGAAAVSTTMPTVQQASPASIPSTSKKYQYIGNKNT
                     KVFHRLTCSSLPDESNRVYFYTREEAIAAGYRPCGNCKP"
BASE COUNT         3333 a         3633 c         3682 g         3211 t
ORIGIN      
        1 gtgccaattt tcttcattag aacctccgct ctcagtgaag ccgtaacacg ggaccttctt
       61 cgacgattcc ggtcaacaag gcgccggttt cgacattcct cgcttggata acctctccga
      121 tttgcgcgtc ctttagagcc tccaccaggc ccttgacctc cacaccgccg atcctcacca
      181 ccgcgaagat cacctgcccc gccttcacgt acgggggatt cgcgatcatt gtgccttcta
      241 tcaccgcgcc ggatttaatg aaggccttgg ccactttcgg aaaggaaacg ccctccacaa
      301 ccggatcccg gtagtcaaaa tagttcaaag gcttgaaaac caccgcgtcg gatgaaaaac
      361 tttccttgta actgatattt ctaccagccg tggctaaatt cctaatccag atgggtttca
      421 ctttccctgt ccagcgtgtg gccggtgaat tcacaggcga gtcggcgacg attttgacga
      481 agtactcgct cttgccgaat ttcgtgaagg tgaggtccca gggtctatcg gagaagacag
      541 agggtggctc tatcgccaat tccagatgaa aagcctccgg cggaatcgca accgaccaag
      601 tctccgacag gaaagccagc gtgagcgtct cgagtcggct cttccattca ttcgggctta
      661 agaacgtctc ggcagggatg tccacagttt cggtcgcccc agtcaaaggg ggcgcggctc
      721 cccaggacga agacgtcggg gtcagacctc tcgagaccgt gatcgtctcc gatcgtatga
      781 tcggttcgat ctcgggaaag tagagcgcga tcttcgcgcg gaggtaatcg gattgaaaaa
      841 ccagcgtctt acccggcaag ggggcgaacc caaacacgat agtccggagt ttttcggaga
      901 gctccggact cggaatgctt tctatgtcga agaaatcggc gaagtaaatc cggctttgat
      961 cgtaaggtag gagtatgagg ggctttacgt cgctggcgaa aaggccggcc cacaacacca
     1021 gcaacaagag gaagccgcag aaccggcagc gattatggct cgaaggaggg atcacaactg
     1081 catgcgcata atctcgcggt aagcgtcgac gatcttgttg cggacttcga gagtcaagcg
     1141 taaggacatg gccgcttttt ccgccgaaat cagcacatcg tggatgttgc tgagccttcc
     1201 ggccgcaaac tcatcggccg cgcgttccgc ttcgtactgg tccgcgttcg tttcttcgag
     1261 agcctgctgt agaattttca taaagtccgg ctctatttcg gacttttccg cttgtcgcgg
     1321 cgtttgtccg acccgggaca ccctctgtat cgggttcgcg cttcctatct tacctatggg
     1381 gtccatcgat tgcgccatgt ttaaccaccc ttccctatct gtaaggcagc gttaacaaat
     1441 gattttgtcg cattcagcgc cgtcacgttc gcctcgtagg aacgttgggc gttgattaaa
     1501 tccaccattt cgcgtaggac gttcacattc ggcatctgga cgtacccttc ctcattcgca
     1561 tcgggatgtg aaggatcgta tatccatcga aaggggcgct tgtccgcttc tatcgcggcg
     1621 atctcgaccc cccgcatcgc gtcggtctgc gctcgcacgt tgcgatagcc ggcgtcgtgt
     1681 atcgcggatt cgtagacttc cctaaacacg accgtcttcc ttcgatacgg accaccttca
     1741 tcggaacggg tagtttccgc gttcgcaatg ttgctcgcga tgacgtccaa acgcgccctt
     1801 tgagcggtta agccggacgc gctgatgtta agggttcgaa aaacgtccag cattagcggg
     1861 ttcccttcaa cacgatatta tacctgacga tgttcaaatc catgagccgg gttaaggtct
     1921 ggtatcggag cgtattcgcg gacaaagcgg ccatttcttt gtcgatatcc acgttgttgc
     1981 catcgttggt gatgacggta tttttaatgc gcgtgaggtc gggctcgact ccgtcgaggg
     2041 agaccggata gttcgggatg tgcctcacac gggaggtttt aaggcgaagc tcatcgccgg
     2101 ccagcgcctt tttgaagcta tcctcgaaag agacctcccg ccgcttatac ccgggggttt
     2161 cggcgttggc gatattgtgg gcgtatactt cgttccgcaa actggccaca tccatcgcca
     2221 gcggtagagt cgtgaagttc gtgttgtaca atcccgccat cgttccctct cctttcggtt
     2281 ctctaaccca tctttctaca gcttcccccc cttttccttt tcttttttct ctgcaaattc
     2341 ccccaaaatg cggggacgga cgaactcggt ggtttcttcg tcgactctgt ctatttattt
     2401 gacaaagtac gtcatatatg gtataatgtc gtacgaagtg acagacttcg tcatttaaag
     2461 gcacaagaag ggagttctga tgacctacgg cgaaaaactc agaaaacttc ggatggccct
     2521 tgatctgacg caagaagagg tggccatacg ggcagattta gcgaggagtt tcatctcgca
     2581 agtggagaca gacaagacat cgccgacact ggacaacctt gaacgtattt tgaaagcggt
     2641 cggttcgacg ctccaagcct tcttttccga agaggaggaa gagaaggtca tctatcggaa
     2701 atcggaagag gtccccttgt acgaccagcc ggtgggagtg cagaccacct tattgatgaa
     2761 cgaggttgaa aataaaaaga tcgacgcgtt gctggtgacg ctgaaacccg ggagtaaaac
     2821 agacgaggag gactaccact ccggcgacga gtttgggtac gttttggctg gaacgatcca
     2881 cctcatccta gacggcgtgg attaccagtg tgaaaaagga gactcctttt attaccgcgc
     2941 cgacaagaaa cacagcgtta tgaatcaaga tccaacggtc aaagccaagt ttctctggat
     3001 aaaaatagat tagcctatca gaaaggagtt gatacccatg aaagccttgg ggaaacactt
     3061 aatagccgaa ttctatgatt gcgacgccga gaccttgaat gatgtccaca aggtcgagga
     3121 aagaatgaaa agcgcggcgt tggtcgccgg cgcctgtatc gtcgattcga gttttcacca
     3181 gttctctcct tatggaatca gtggcgttgt agttatttca gagtcgcatc tgtcgatcca
     3241 cacttggccg gaatatgggt acgcggcagt cgacgtcttc acttgcggcg atcacgtgga
     3301 cccttgggtc gctttcgatt ttttgaagaa ggcctttggg gcgcaacgct ttctgatcga
     3361 cgagtaccaa agagggaact acaaagctct gggcatctcg gaaggggcat cctataaggc
     3421 ggttgaagcc acggcttgat atcgtcttca aaccgcctta tttctggccc aaacactcga
     3481 ataaaacaga gactggaggg tttcacatga acctaagcga aaaaagcccg ttctatttcc
     3541 gagaggtcta ccacgataag aaatgttttt tggagttgac cgtattgcgt tgggtccact
     3601 ctcagaccac ccgttttcag aagatcgata ttttcgacac gccctcgcac gggcgtgtct
     3661 tcgccttgga gggcatcgtg caaacgatgg agcgttggga gttcatgtat cacgagatga
     3721 tcgcgcacgt gccgatgatg atctgtgaac ggccgaagca agtgctcgtg atcggaggag
     3781 gagacggcgg aaccgtacga gaggtgctca ggcatccctc cgtggaaagg gtcgtcctgt
     3841 gcgaagtcga ccgggaggtc gtggaagcgg ctaagaccta tctgccgacc ttgagccacg
     3901 ccttctccga cgaacgcctt tcctgcgtct ttgcggacgg agcggagtat ctgaaagggt
     3961 ctcctcaaac ttgggatgtg atcatcatcg attccaccga tccgacggca ggcgaaggcg
     4021 gcaatttgtt cacagaatcc ttctacgcgc tgtgccgaga tcgactcctg cccaaaggcg
     4081 tctgtgtggc ccaaacggag aatccgatct atgaccgcga atggatgcga acggcttacc
     4141 gccgtatcgc aggggcgttt ccaaccgcgg gcgtctacct cggattcagt ccgcaatacc
     4201 cgtccgggtt ctggacttat agcatcggct atcgttcgga agaaggacag agcgcgccag
     4261 acttttaccg gctgtccccg gaaaagatgc ggcgaatccc ccctcatttg cgctattata
     4321 cggctgacgt ccatcacgcc gcctttcaga ttccgccctt cctgtccgac tatttggcct
     4381 gatccgccca gcccttatcc tcaacaaaaa aagaacccat atcgggttct ttttttctgt
     4441 gcaggcgccc ttatgtgatc agaatcagtc gttgggccat cgtcgacggt gggctccgct
     4501 atcacacggc cgggtagacg ccattctcga ctgtgtccgc gatcgactcg tctataccga
     4561 tgcgtccgta gtacttctct ttcaaaaagt tcagtgccaa gtctccgaac aaggcgatgc
     4621 tgagcagctc ttcttctttt tccacgaaag accccaacat cttgcgcagc tccggcaatt
     4681 ggggcggtat caggtcggcg ggtcgacagt cgatcacctc ggaagggtct ttgacgatca
     4741 cgcgttttag gtcgggatcg atgtcagccg gcggttttcc gtagtggcct ttcacatact
     4801 gctccgtctc tttcgtgatc cgtgagtacc gttttcccgt caacacgttc agtaccgcct
     4861 gtacgcccac gatctgactg gtcggggtga cgagcggggg atacccgagg tctttgcgca
     4921 ccgagggaat ctccgccatc acatcgttta agcgatgcat cgagttttgc ttttttaact
     4981 ggctatagag gttggagagc ataccacccg ggacctggga cgagagcacc ctcagatcgg
     5041 tgtgcttgat gttgacgtcg tcggtggcgt gtttttggcg tacggtctcg aagtaatcgg
     5101 atatctccag aaagtgttcg ggatttaagt ggcaacgggt ctgcgggtat tctttgaagc
     5161 tataatagaa gctctccacg gctggctgag aggacgcccc ggacatagag gaacaggccg
     5221 tgtcgaacag gtcagccccc gcctcgaatg ccgaaagata gctgagttcg ccggttccca
     5281 ctgtgcagtg tgtatgcatt tccacgggga tatctttttt ggatttgatc atcttgacga
     5341 ggtcgtagga tatcttggga gtcagcaatc cggccatatc cttgatacag atcgagttcg
     5401 gttcgtacgt cagtatgcgg tcgaccagag aagaatagta ttccaacgta tggacggggc
     5461 tggttgtaaa acagataccc acttggatat gccccttgta cttttgggtg tacgcgatag
     5521 acttttccaa gtttcttagg tcattgagcg cgtcgaaggt tcggatgcga tccaaaccgt
     5581 tcttgatcgc ccgcttcacg aacagttcca agacgtcgtc cgcgtagttt ttgtaaccca
     5641 cgatgttctg ccctcggagc agcatctgaa gttcggtgcg tttgatgttt tttcgtatgg
     5701 ttcggagtcg ctcccaaggg tcttcgccca aaaagcgtaa gcagctgtca aaggtcgccc
     5761 caccccatac ttccagggat tggtagccag tttggtcgat ggtcttcagg atcggaagca
     5821 tctcttctgt gctcatccga gtggccaaca aagactgatg cgcgtctctt aaggtcgtat
     5881 cgacaaagta cacatctctc atcgtcttct ctcctcatag gtcggtgtat ggataattta
     5941 gtctcttcac tcctcaaaga aagcatagca taaatatgtt tcaattctga aggtgcgttg
     6001 gtaggagcgc gtgtcagtcc gaagttagcg cgctacgaac caagctccgc cctcacgcct
     6061 actaaaggcc agcgccacaa tctaagcggg aggtcgttta taatcctctc cccgttaaga
     6121 taccctcttt gcgagaggag acgtttggcg ccagggttag gacagctttt caaagatata
     6181 gaacttcaaa tagtccgttt cgaagatatt cagtaccacg gggtggtccg cgggttgacc
     6241 tcccctgtag acaaggcgaa ggcgcgcccc tacgtctgtg gccgcggagt acatcatcat
     6301 ctcgaattct tgcctggaaa cagcttgaga gcaagaagcg gagaccaagt acccgcctcg
     6361 tctcaaggct ttcaacgctc gaaggttgat ctctttgtac ccccgcaacg cgtttccttt
     6421 tgaagagcga gacttagtga acgacggggg gtctaaaacg atgatatcgg ccttctcgtt
     6481 gctctcttcc caggcacgca ggatatcgaa ggcgttacct tcgtgaaaag aagcgccgct
     6541 caacgcattc aaacgtaggt tttctcggca gtcggctatc gcggtcgcag attggtcgac
     6601 gaaggcgaca cgtttcgcgc cgttttgcag catccggaga ccgaagttgc ctgagttgga
     6661 aaaaacgtcc cacacgacct ttccggctgg gaagcagaga gaagccgcga tcacggcgtt
     6721 ttcgagttgg tctatataaa tccccgtttt ctgcccttcg ctcggagaaa ccgagaaccg
     6781 taacccctga tacgagacgc taaaaggctc gggaaattct cgcccaatcc aaccattcaa
     6841 cggcgtcacc tgctccttcg ccagcgcgat agggtcgctt ttatcgtaaa agaatccttc
     6901 gggaaagcgc tcgcgaaccg cgtcgatgat ctcggaacgg aagcgatgca tcccagccgt
     6961 attaaattgg aagacaaaca cgtcttccaa gcggtcgatg atgagcccgc tcagatcatc
     7021 cccctcgccg aagaccagac ggcggaccgt agaccgcggc gtaatggcgt ttcggtaatc
     7081 gatcgcttca gcgatgcgct tgaaaaagaa gtcgcgatcg atccgctcat ttctacgggt
     7141 gagaatccgt gcgacgatct tcgagttcgg gttgacgtat ccgcgcccta tccactgcct
     7201 cgaaaatgaa aagacatcga ctgtatcacc aggcgtgatg tcgccttcga ttcgttctat
     7261 ttcattgtcg tacacccacg gatgcccgtt ggctaacctg ggggacagca gtcttttcaa
     7321 gatgattcgt gcgtccatat ccggtccttt ctcaaacctg tgtagaacgg ttaaatgcgc
     7381 actttcggct gggaatttcc tatacattcc cgaagtcgga ctattgccgc gacaccggat
     7441 ggccagggag tgatccgatc gcagtttcgc gtctttaaaa gggcctcctg cgctccctca
     7501 acgttttcgc gcgcgagccc gtccgagccg atcgaaactc cgaggcgacc cgttctctaa
     7561 tcgaccaaaa agcgcggtgt cagcgtggaa actttcctgt gcctatcggt accggccgat
     7621 tataacacac ctttcattga gtgtgggtat aatatcaagg tgcaacgaaa aatagaaaga
     7681 ggaggagatc ggccttttgc cctttttacg ccgcgatgat cgtgtgtgtt atcgcggggt
     7741 aagtggtttt tacgaccccg cgattcgccc tttacctttt tacgccacga tggctacgtg
     7801 gactaaaaat cgagcgatcg attctggcct tgtttctcga tgacacctca taactagaca
     7861 cacctttcat tgacaactcg gatatagacc tgtataattg tacgaacagc gttcgaatat
     7921 ccttcgagaa ggtgaaaagg tggaagcctc cctccgtgaa cagatgcgat gggtattcca
     7981 gatgatcact cgcgccttgg tgacgggaca gatcagcttt cgagacgacc cacaactcta
     8041 ctccttgtac ttcgagaatc acgacatacg cgattcggtc cggcttttcg cacaagaagc
     8101 caggctgttg gttcgggaag acacccaagc gatctatttt ttttgcgccc aagagagtcc
     8161 gttcacggtg aaaaagacgg aattctttaa acaggtctcc tttccctcaa aacgatggcc
     8221 actgtttgag gtcatcaccg cgtgcctttt tctacgattt ttcgaagaaa acgcggagta
     8281 cgtggttttg tccgaaatcc tagagcgcct tcaaaaatta aaggagaaag cgcgccccga
     8341 atcgcctgac cgttctccca agaagcaaga tgcggccgcg gtgcgttgga tgaccgactg
     8401 ggaaggactc ccggaggggt tttttcggcc aaacgactgg agcgtataca agagcgactc
     8461 tcaggtcgga ctgatcgtta aaaccgttca ggtgctcaaa gaacaagggc tcgtcgcgga
     8521 atacgagaat cagtggacgg tcataccgac gggaaaagcg cgcgcggtgt acggaatgtg
     8581 gagagaagag ggcgcttgga acgctctagg cctcggagat accgaagatg cctgagctta
     8641 agggatttcg ggttctcaac ctcgcctatg gagagacatt tgtcatcccc gacctcaccc
     8701 tcgatttctt cgggcgtaat accctcatcc ttatgcctaa tggcacgggt aaaacctatc
     8761 tgctctcttg tttcttccag acggtccttc cgggcgctca cacgccgtcc cgaagcgtgg
     8821 aggatctgtt ctcgagatgt accggaacct cccatatcct cacggctttt tcaatcgaaa
     8881 aagaaacggt tttcctcggc ttttgtaccc gaaagaaaac ggatcggacg tatttcaatt
     8941 ggacgctacg actcgatgaa ccgtgcgaca tcgatgcgct gccgatgcgg aggggtgagt
     9001 cggtgctcga ttacgatgag ctccctttgc ttctgaaatc cctgcacaaa gacagattca
     9061 ccctctttcg acaagaggca cggcctgcct acgaacagca gttggaacga tatggattgg
     9121 ggacggagga gtggcggttg atgcgcgctt tcaacctttt tttagaaggc gcgctacccg
     9181 tgcaagaggt attgcccggc gcttccgaca gactcctcga tccgatggtc tttcctttag
     9241 tcgaaaagcg cctaaaaatg gacggattag acccccgaga gagggtggac gccttttttt
     9301 cgtcccagaa cgcgataaag ccaagcgcga ccgcaggttc tgtcctcgcg ctgaaagagc
     9361 ggctccaatc ccggttatct acgctggatg actttcgaga agcaaaagaa gagctgaata
     9421 acgcggttta ctctcttcgc acgatacagg agttcaccca taaagagata cggaaagcga
     9481 aggccgagtt gcatcggcta ggggcccgac tcgtcgcttt ggtggaggag gaagctcagt
     9541 gcgatcgaaa tcgtcaagtg cgggctctgc aagaaagtgt cgaaaaaaaa gcggagcttg
     9601 agcgaacgga aacccggctg gtcggcctta tcgaagaaaa gcaacaaaag atcgcgctcc
     9661 tgtacgaaac cttacgccga cgcttgacaa gcgaacctga tcctcatgat tccgattggc
     9721 acaccgatgc ctcgctcgaa agccgaaagc ccgtgtcggt tccttgggcg ttgatggcca
     9781 aacatcatct ggaatcgctt ctggaaacgg ttaaggccca tcaaaccgag agagaaagcg
     9841 ccttgcgctc ttcttgggaa atcttgtcga tacagcagcg ctgtgtcgcc cttcaggaac
     9901 gctacgaaag gttgggaggg ttgacgcgct tccaaaagat cgggtacgaa aaagagaggg
     9961 cgcgcgccgc gttggaacga aatcgacgga gacaatcgat acaaaaggtc ttgagaataa
    10021 agcttcgcca gaaacaacgc accctcgagg gcgacctaga acgtctgaag gcccagctcg
    10081 ccgaggtcga tcggaacgcg gagggaatcc aaaactatcg cgagttcgct caagcgctaa
    10141 cggttttgga gggcgcgcgc cccctacccc tcttagccga gcggttggtg gagaagcgaa
    10201 accgtatgtg tgttcggata gcggaagagc gtggggagat tgaacggctc gatcgtctgg
    10261 acgcgaaaga ggccgaaccg atcggcgcgc ggcccccaaa agaagtggaa aagctatccg
    10321 cagccctcga gcgcaacggc ttttccggtt tcgaaaaggg ggcggattgg attgggagtg
    10381 gaaaaagcga caacccgttt ctttcctttt cactgatcac ggaataccct ttggagatgg
    10441 accaagcgct atctcgccta ccctttgagg acttccctac cttcccgatc ccggttttat
    10501 ccgtcagaga ggcgacgaac ttggccgagg caagcgaata ccggacgatg atgcgactga
    10561 aattgcggtg ggtgccttcc ctaacggagc gggcgctcca actccgcctc agcccgggta
    10621 tgcgcgcctt cgcagaaaag ggccccgttg tcggatcgga cttagcccat tggctgcagg
    10681 cccgccgggc cagtatacgc gctcggatag aaaacctcga agggacgttg aaaaaaaccg
    10741 agcgtcaact ggagcgcttg gcctatttca tacaggcagt cggttcagat ccggaggtcg
    10801 cccgagagac cctcgctgag aaacgctctc tcatagaaaa agacctatgg cgggtatcca
    10861 aaagtctcgg cgacctcgga gagaaaatac gcgcctcgag actgctttct acatccctag
    10921 acgaagagga acgggctcta caacaaaaga tcgagacgct cgagagagaa gagtgggaat
    10981 ggtcgcagct ttcctctgag atcgatcagg cctgccaggt gccgttaaag gacttttcac
    11041 tcttttgcct atcgatccag aaacgtctcg ccgaatggga cgcgaaggtt caagaagcgg
    11101 aacgggtaat aggagagagc ctcgaggagt taagaaaccc ttggctttct ttggtcgccc
    11161 gagaaggaca agccgcgctt acgactgtcg aagaacagcc tgaggcgctg tgggagatcg
    11221 ttgcgcggat ccgcagcctc gagagtgaat tacgagaact tttacaggag cgggcggtcc
    11281 tccaggcgag gagccccgcg cttctcgatt ctagcctctc tcctaaccta tcgcgcgcga
    11341 tcgaaagggg gtctcaggag gacccacttt ctcagaccac ggagacgctc gaaaccttgg
    11401 gaagaaggtt acgagagatc gaaaaagagc ggcaagagat cgcacaggag acagctgaaa
    11461 tagtagagac gctgcagcga ctggaaaccc tcgcgcaacg acagcgcatc tttctggcgg
    11521 gcaaagagtc gtcagaaaaa aagtatatct cgggtctccc ccaagtcttc ttaaagagcg
    11581 acctacacgg cctttggacg gaaaaggaag ccgtatgtct caaacaactg tatgcattgg
    11641 atcgacaaca ggcagctttg gacgaggatt gggccgatct gaaaaaggcc ttagacccca
    11701 ccgaaagctg ggaagagccc gttctcgaca gtccgggaga agaagagctc gaaggcggca
    11761 aaacagttgt cacgcgtctg ctacacgggc taaaaaggcg gatcgacgca gagcgagcgg
    11821 aagaaagaag ggcccaaagc gcgctgtcgt gtttttctag agagattggc gacgcgatgg
    11881 aagtcgtgaa cgcgcacctg agggagatcg cgcgtttttc gaaacccgag ctcgaacggg
    11941 cgcctttgct cgagttctcg cttccgaata agacgcggga agagtacgac aagagctttt
    12001 ccagtcggct cgcggaatac atcgcccttc aacgggcgag ggggcaggag attcgcctac
    12061 agtggccctt gcgaaccctt tgggagttct accacgacga gcggttggaa atccgcattg
    12121 aaaagccccc tttcacgagg gagggagaag gcaagcacgc gtggaaagag gtagagaaat
    12181 ggtcgactgg agaaaagatg gccgtctacc tcgcgctgta cgcttcccta ttcgcctggt
    12241 ggaaggggca gacgccgaac acgaaaccca cctcgttcct cattctcgac aacctgttcg
    12301 ggcacatcaa taccgaaacg ctactagaaa tccctatgcg gatgctctac acccacaagt
    12361 ttcaaatcat cggcttcacc tcatcggcgg aacccttcct gttcaaatac tttccgaaat
    12421 ggatcggcct cagcccccaa gaaaccgatc actacagttt tttcttgccc tttcacgttg
    12481 aaagataaaa cctcatcgac gcttccggga gcgcaatcgg aataccgttg aagcttcgga
    12541 ttttcgtttg gcgagttttc gctgggaacg cctggaaacg tctgggaacg ccgattttta
    12601 agagagtatt gcagtaagcc caagtagagc attgggagtc aatgatagag ttctgaacga
    12661 gtatatctta aggcttgcag ttcccacacg gcctataacc cgcagcaatt gcttcttccc
    12721 gtgtgtaaaa atatactcgg ttactttcat caggcaacga actgcaggtt aaacgatgga
    12781 agactttggt atttttgttt ccgatgtact ggtatttttt agatgtgctc ggaattgatg
    12841 cgggacttgc ctgttggact gtaggcatcg ttgtagagac ggcagctgct ccccaaagac
    12901 catacccgtt ttctcgagct ttctcctgcg catcagaaaa catcaccatg taatcttcac
    12961 ggaaggaaaa atctgagcac gcgtacccat acccgttttc tatgatggca aggttggcca
    13021 aaacccattt tccatcatcg cctttccacc acaaataggc cagaagctgg ttatttacat
    13081 ctctgggatt ccggtcatag ctcagataga cctcctgacc atccttacag agttttttca
    13141 gaaagtcgga agcctctttg ccgaatgctt caacagtttt ggtaggatgg acagtctctt
    13201 gtgtggccac tcctattagc ctcaccgatt cagttcctga agctaatgat acttttaccg
    13261 tgtttccgtc tacgacactc actacgcgag caggggctgg tgtaattacc ctgtaagata
    13321 gatccatcgg aatgagatag gtgtaggagt atacgtaagc aaccccattc cgcgtcaggc
    13381 cgaaagcaaa agacaaaaag aatacacaaa aacagataca ggaaatgatc gtttttttca
    13441 ttcaacacct ccggttagta catcctccaa aagaatatac caattcctcg cgtgggaata
    13501 atatagggat ctcacaattt gataataatt caacttgtgc gtggtcatta caaaaacgag
    13561 aaaagcgaaa aactgaatga aatcggtctt tgaaaagaaa gcgaacaagg aataaggaaa
    13621 atcggtaccc catctcaaat tattaagtgt gaacaaaata acaaggagga ggggtaccga
    13681 catgactatt gtaccactaa taagacaggt tgtccaggaa aggttcggaa cgtttttcga
    13741 cagcatcgaa aacttttatc tgaagaactt tcttatcctt atccaaggct tactcacgaa
    13801 ttcttatacg agtataagcg ctatcgcggc agatgaacac tacggcgttt cccatacca
//
LOCUS       DAOPXN010000053        13828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4518_length_13828_cov_5.013940, whole genome shotgun sequence.
ACCESSION   DAOPXN010000053 DAOPXN010000000
VERSION     DAOPXN010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_13828_cov_5.013940"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            639..1862
                     /gene="pgsB"
                     /locus_tag="PKM49_04430"
     CDS             639..1862
                     /gene="pgsB"
                     /locus_tag="PKM49_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HNY82094.1"
                     /translation="MFILLTASCLVAILTYLIYEQRQLKKCYRAIPIRVCVTGTRGKS
                     SITRMITWLLQREGLPTCGKITGTNARFLRPDGTEEPIVRKRPASILEQKRMLRRAAQ
                     EGAYAYVSEVMSVTPEYQNTETKRILSPTHLVIANVRADHLSVTGASLEEIGSVFFRS
                     APTHTLIVTTRDVATRYGANRPSSQRVISVEAEHFPFDPLDFSYPEFPENYALAVSCV
                     EEVLKSLSEFDSGRPVPKENLTDPRTISMKGLRSDFGVLRAWKAYGKVFVSGFAANDP
                     ESTESILQACERAFGWPRNEAIGWCHLRIDKGERSLQWLEEWQQKGIPFRKLYLSGGH
                     AVAVKRRLRNPKVSLLREAPMEHIVKILSEETSPAVFGFGNIKGFGARFVEYLSSDPA
                     RFPPCSIQVTKERLP"
     gene            1859..2302
                     /gene="pgsC"
                     /locus_tag="PKM49_04435"
     CDS             1859..2302
                     /gene="pgsC"
                     /locus_tag="PKM49_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HNY82095.1"
                     /translation="MIQINTLVGLTLSLFFTEWFGLYPGGIIVPVYLSLYVEQPLRIV
                     GTLFISLATLLLYRWLCRYVILYGRRRFILLILIGSILNFSWTFFSGALFAEALRLQG
                     IGWVIPGLMANAYERQGIGKTLLAMAVVTLSIFFSIRIVTLFFFT"
     gene            2314..3408
                     /gene="pgsW"
                     /locus_tag="PKM49_04440"
     CDS             2314..3408
                     /gene="pgsW"
                     /locus_tag="PKM49_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNY82096.1"
                     /translation="MKAKDIWLMLGLAVSFFFLFRFVWYRQENDDYAEMHRARQEMVQ
                     ALQIVRGERLNRGIPIDLVTDPNQTGIIGYEYSEITSTPGELEAKRTTTNPDMAALMV
                     SFFKELGLSRGDIVVVGSSGSFPSLYIACLSACEAMGLAPLSIISLMASEYGANIPGF
                     NFLDMVTALKEAGKTAFYPGAVSIGGESDSGKGVSSDVIGKLETQIESSGLQYFRNPS
                     LKEGTQERLDWLNELIGERPIKAFVNIGGATANVGTDPIFLYLKPGVNHISQYAPFET
                     GGLLYALARQGIPVIHLLYVKGLVAQNGLPWDPIPLPSELSLQRKLLGVSEKAAMATL
                     FLAYWAIALLLRLRGARTLRHRLGLFDRQR"
     gene            complement(3346..4407)
                     /gene="tsaD"
                     /locus_tag="PKM49_04445"
     CDS             complement(3346..4407)
                     /gene="tsaD"
                     /locus_tag="PKM49_04445"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNY82097.1"
                     /translation="MSNSVPKARRYLLGIETSCDDTGVAILDDEGKVLANELLSQIDA
                     HQVFNGVVPEIASRKHFETIHTLLDRALESAGLSWGDLSAVACTIGPGLIGSLLVGVC
                     TGKAVACALGIPFIPVNHLIGHIFSNALTHPELAPPFIVLLVSGGHTDLYAVVSYEDL
                     RHLGGTLDDAAGEAFDKGARILGLPYPGGPSVSKAAVTGDPHSVRFPLPLKKQGTMDF
                     SFSGLKTALLYHMKKHPDSSVSDAAASYQEAITDILVEKTFLAGRQTGYRKLVFAGGV
                     SANRALREKARSRAAKWGYTVFFPRVEDCMDNAAMIARAGWHALWSGAVHQPKATVDS
                     SLTIEKTQAVTKGSGSSKA"
     gene            complement(4400..5104)
                     /locus_tag="PKM49_04450"
     CDS             complement(4400..5104)
                     /locus_tag="PKM49_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNY82098.1"
                     /translation="MEHIKENLERLKTEIAKICDKHKRPTSDITLVAVSKTFPPEAVV
                     AAYACGHREFGENRPQSLRDRFLEIKEEGISWHLVGALQKNKFKYVVGKTALIHSIDS
                     AETALGIDEFCRKRTLTQDILIEVNTSGEWNKMGVSPALLPELLEATRSCQHIRITGL
                     MTMAPLTSNEKVLKKSFSTLRTLRDTYQKSYPELKHLSMGMSNDFQIALGEGATILRI
                     GTAIFGARTEPKTFRV"
     gene            complement(5184..5564)
                     /locus_tag="PKM49_04455"
     CDS             complement(5184..5564)
                     /locus_tag="PKM49_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNY82099.1"
                     /translation="MTAKEQVYKCEKCGNIVEVVHSGAGTLVCCGEPMKLMVEQTAEQ
                     AKEKHIPVLEKLEKGVKVVVGSTLHPMIEKHYIEWIELVTDHGVHRKYLKPGGAPEVT
                     FDCGCQIKQVREYCNIHGLWVLNL"
     gene            complement(5578..6750)
                     /locus_tag="PKM49_04460"
     CDS             complement(5578..6750)
                     /locus_tag="PKM49_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNY82100.1"
                     /translation="MAIKKISDTIYNIGVIDWSRRLFDELIPLPDGTSYNAYLVVGSE
                     KTALIDTSDPSKPEEFRRGLEGLPVKKVDYVIANHAEQDHSGLIPLVLKKYPGAKVVT
                     NEKCKEILLEHLPLKTEDFIVVSDGQTLSLGNKTLRFFFAPWVHWPETMLTFLVEDRI
                     LFPCDFLGSHYATSEVYSDDSQRIYRAAKRYYAEIMMPFRSFVQKHLATVRGLDPVLI
                     CPSHGPVYKNPNFILSAYESWVSDDVEPRVLIPYISMHGSTEAIVDHLVNAFVQKKME
                     VIPLNMSVSDLGELAIDIVDASTLLFATPTMLLGPHPQMLYTMVLTNALKPKTKYLSL
                     VTSYGWGTKVVEAAKGALGNLKAEWVDPITIKGFPKAKDYEAMDRLVDLIVKKHHT"
     gene            complement(6772..6930)
                     /locus_tag="PKM49_04465"
     CDS             complement(6772..6930)
                     /locus_tag="PKM49_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNY82101.1"
                     /translation="MERYVCTICGYVYDPEIGDPAGGISAGTPFQDIPDDWICPECGA
                     GKDAFEKE"
     gene            complement(7119..8264)
                     /locus_tag="PKM49_04470"
     CDS             complement(7119..8264)
                     /locus_tag="PKM49_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNY82102.1"
                     /translation="MDLLQRALVEHERWKGKLSVESKASVRSMDDLSIAYTPGVAEPC
                     KEIHKDREAVYRYTMKGNCVAVVTDGTAVLGLGDIGPEAALPVMEGKAVLFKEFAGVD
                     AFPICLNTKKVEEIVQAVEWIAPVFGGINLEDISAPRCFEIERTLKSRLSIPVFHDDQ
                     HGTAIVVLAALINALTVVSKKAEDVKVVISGAGAAGNAVAFLLKTYGVKDITVCVRAG
                     IIHREAPWLNWAQRELAEFTNLVQKTGTLADAMRGADVFIGVSGPNIVTTEMVASMNS
                     NAIVFALANPVPEIMPDLAKAGGARVVGSGRSDYPNQVNNVLVFPGIFKGALKARKQI
                     SEPMKLAAAKAIASMIAPGDLREDSILPKPFHPGVADQVARAVLESE"
     gene            complement(8270..9391)
                     /locus_tag="PKM49_04475"
     CDS             complement(8270..9391)
                     /locus_tag="PKM49_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HNY82103.1"
                     /translation="MLDDRVVWWPFEDAKVFVEAVFCAAGIPNEDAKTCTEILLESDK
                     RGIESHGIGRLKPIYIDRIREGTLQPVTKTEIVRESPATAVVDGHNGMGFVVAKASME
                     LAIKKAAVYGIGMVATRHSNHYGIAGYYPLMATERGMIGITGTNARPSIAPTFGVENM
                     LGTNPLTFGMPTDEDFPFLLDCATSITQRGKIEVHDRAGKDIPEGWVIGHDGRIRTDT
                     HQILSDLVSGQAALLPLGGPGEALAGYKGYGYATVVEILSASLQNGYFLKMLNGFDEL
                     GNKRPFGVGHFFIAINVESFIELEEFKRITGTILRELRASKKAPGADRIYTAGEKEHL
                     AWLERKDKGVPVNTTLQKQMSGLKETYGLSDLPLPWEKR"
     gene            complement(9408..10562)
                     /locus_tag="PKM49_04480"
     CDS             complement(9408..10562)
                     /locus_tag="PKM49_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNY82104.1"
                     /translation="MSLSPVYLEIAKGISIFSVIAGHSLAAVINDMPVLEQLLFSYLG
                     KSMVPAVFCLIYLFGYGQGLKRKRVTTRMNLERIVDMVKPYLFWATLSLLIYTVSDSR
                     VTYPYTGREIFGYEITPLSFVLSILTFSGSWQYYFLFILILFQYFTGILRNVHPELYP
                     KLLRRAFIFQTTVLAILSVSLWVIPQEKIPLLSLGALLYPNPLLWFFPFFWGYLRSAS
                     RKPVFPSFGKRGVYGYLILLALTGTEAFLFVRRWQTYFVIDQFSVFTFFLSIFALSCI
                     GSVSHRLEKRWHSLRARKETLPRPLREKLGVGVLSIFIRFGRFSFVLFLTHQPFMWFF
                     LVWFEEAIGYRFSRSVEFLLMIFFGILFCWGIIKLSDFLPKSIRRFVIGF"
     gene            complement(10597..11823)
                     /locus_tag="PKM49_04485"
     CDS             complement(10597..11823)
                     /locus_tag="PKM49_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY82105.1"
                     /translation="MFWFKWISIIAVYPLVYAFSRIFSFGSYVFPCLLASVGAFFLVF
                     YTSRKRFLEQMTLEALPYEESLFHEPRIAVLFVVKNEGERFIENVRDYLSLAGPIEVT
                     VYDDHSEDQTVEMLRALQREIDPVTGKDYRDRLHIRSVPASEKRIHPKGIALEEAFHT
                     IECDLFFVGDADTAISKETFSKALGYTLREGYDVLHLTRRNVEHGTLSYSIADADEIF
                     VSGLQLLNMIPFNFTGSSFFISSKVASKINFPSQVYSEDSEIGRQANAFARKKGFSIS
                     CHTWERAPQTLFLLLKQRWKWNKYAMNQYFEQDFPVILLAVLLSANLIFGLLSLYSFN
                     FLCTLVLAFCVLAFSLSSNLYIASKKLSVAFFSSCAYILMMLFQFGILLFILILFLPK
                     DQRLGIRYVKTETTAK"
     gene            complement(11835..12863)
                     /locus_tag="PKM49_04490"
     CDS             complement(11835..12863)
                     /locus_tag="PKM49_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82106.1"
                     /translation="MQVNMRSISGTKKGHGVDSAYNDLVRLLERGGEFQVLKNTDSLT
                     DILHLHTVDLKSLRLIRHFHKRGKGVVITCHMMEDSLDGSLSLPKFVVKYFGKYLYYF
                     YRQGDRLVVVNPYYREELISKGLDPAKVSFIPNYADERLFFEVNEEKKAALRRELDLP
                     LKAPVIVSSGQIQRRKGFDDFVACAQLNPDYLFVWVGGFSFGRLSDGYDTYRAIVADP
                     PQNMRFPGIVDREVVAEYLQASDLFFLASFQELFPMSLLEAAKCGLPILLRDIPLYPG
                     ILFGHYYRATDVQSFSDWIRRICQDEAARRKGIEDARRLASIYSETAIYKQWKALYEE
                     LRPGRTTV"
     gene            complement(13280..>13828)
                     /locus_tag="PKM49_04495"
     CDS             complement(13280..>13828)
                     /locus_tag="PKM49_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNY82107.1"
                     /translation="RPSNKKEVLPLKGSPFLLFSLLVLPIVAFALLCIGYSVFKLPYL
                     PQIVNSSGKYGIDPLLTAAVIQTESGFDSLAQSPAKAFGLMQLLPQTAAWLEESLPIV
                     GSWREPHNNIELGTRYLRYLADMFEEDWDIVLTAYHQGPTYIRALLEAGGWQKTAYVR
                     KARVFYLCFFLLYHGYFSVLGD"
BASE COUNT         3295 a         3756 c         3588 g         3189 t
ORIGIN      
        1 gtagatagtg ttggaggaaa gacttcgcgg tttctatatc ccccaagact tctttaaaaa
       61 atctgtcgtg tgacttactg attttttcca cggatatccc tccagaagaa agtctcatcg
      121 tatcatacct caaaggcggg atttccaaaa agaaaaagcg tctccgcctt tttttctttc
      181 tcaaaagcgt atcgcgggcg tcgcgaaaag caaaagcgct tacgccgcga tgctgccttc
      241 gagaatgctc ccagtgaaaa ggaaaaacct gccaaactaa agtttggcgt tcccagggcg
      301 ttcacaagta tcttttcaca gtgttttctg tgccctccgt gcgaattctg tttttagata
      361 tgccaacttt aaagttggcg ttcccagaaa ttctgccgaa ctgaagttcg gcgttccaag
      421 acgtcccctg agaaaggccg actggaagtc ggccccacga agagaagggg cgcttacgcc
      481 gcgatgacta catagactaa aaaccccgcg gccgattcta accttgtttc ttgattacac
      541 cccataactt gacacacacc ggcaaaatcc tcgaacccgg gactatgtta taatcgagag
      601 gtctcaagat catccatccc ataggaggat gagcgtcctt gttcatacta ttgaccgcta
      661 gctgtttggt cgccatcttg acttacctga tctatgagca acgacagctg aaaaagtgct
      721 atcgcgcgat ccccattcgc gtgtgcgtta cgggtacccg cgggaaatcc agcattaccc
      781 ggatgattac atggctccta caacgagagg ggttacccac ttgcgggaag atcacgggca
      841 ccaacgctcg ctttctgcgc ccagacggta cggaagagcc gattgttcgc aaacggcctg
      901 cctcgatcct ggaacagaag cggatgttga gaagagccgc ccaagaaggg gcttacgcct
      961 acgtaagcga agtgatgagc gtcacgcccg aataccagaa taccgaaacc aagcgaatcc
     1021 tctcgccgac ccatctcgtc atcgcgaacg tccgagcgga ccatctatcg gtgaccggcg
     1081 cttccctcga agagatcggc tctgtcttct tccggtccgc cccaacacat accctcatcg
     1141 tgacgacacg tgacgtggcc acacgctacg gagcgaaccg tccctcatcg caacgcgtta
     1201 tttcagtgga ggccgaacat ttccctttcg acccgctgga tttttcttat cccgagttcc
     1261 cggaaaacta cgctctcgcc gtttcctgcg tggaagaggt cctgaaatct ctctccgaat
     1321 tcgactcggg ccgaccggtt cctaaagaga acctgacgga ccctcgaacg atttcgatga
     1381 aggggctgag gtccgatttc ggcgtccttc gagcgtggaa agcatacggg aaggtttttg
     1441 tcagcggttt cgccgcgaac gaccccgaat cgaccgagtc catccttcag gcttgcgaac
     1501 gagccttcgg ctggccccga aacgaagcga tcggctggtg ccacttgcgt atcgacaagg
     1561 gcgaaagaag cttgcagtgg ctggaggagt ggcaacaaaa aggcataccc ttccgaaaac
     1621 tctatttgag cggcggacat gcggtagccg tcaagaggcg ccttcgaaac ccgaaggtga
     1681 gtttgcttcg cgaagcgcct atggagcata ttgtgaagat cctatccgaa gaaacctctc
     1741 cggccgtctt cggattcggg aatatcaaag gatttggcgc gcggttcgtc gagtacctct
     1801 cctccgaccc ggcgagattc cctccctgtt cgatacaggt cacaaaggag cgtttaccgt
     1861 gatacaaata aacactttgg tcggtttaac cctctcgctg ttttttacgg aatggttcgg
     1921 tttgtatcct gggggcatca tcgtcccggt ttacctctcg ctctatgtgg agcaacccct
     1981 gcggatcgta gggacccttt tcatatctct ggcaaccctc cttctatatc gttggctttg
     2041 ccgctacgtc atactctacg ggagaaggag atttatactg ttgatcctca tcgggtctat
     2101 cctaaatttt tcgtggacct ttttttctgg cgcgctcttt gccgaagccc tacggctgca
     2161 agggatcgga tgggtcatcc cgggactgat ggcgaatgcc tacgagcggc aaggaatcgg
     2221 caagactctt ctggcgatgg cggtggtgac gctatcgatc ttcttttcta tccggatcgt
     2281 caccttgttt ttcttcactt aagggggctt tcgatgaaag ccaaagacat ctggcttatg
     2341 ctgggcctgg ccgtatcctt cttctttctc ttccggttcg tgtggtaccg tcaggaaaac
     2401 gatgactacg ccgaaatgca tcgcgcgcgc caagagatgg tccaagcgct tcaaatcgtg
     2461 cgcggggaac ggcttaaccg cgggattccg atcgacctcg tcaccgaccc caaccaaacc
     2521 ggcatcatcg gctatgaata ctcggagatc acctcgacgc ctggcgaact cgaggctaaa
     2581 cggacgacga ccaatccaga tatggctgcg ttgatggtct cgtttttcaa ggaattgggc
     2641 ttgtcaagag gggatatcgt tgtcgtcggt tcttcggggt cgttcccttc gctctatatc
     2701 gcctgtctca gcgcttgcga agcgatggga ttggccccgc tatccatcat ctctttgatg
     2761 gcctccgaat atggcgcaaa catcccgggg ttcaactttc tggatatggt tacggcctta
     2821 aaggaggccg gaaagacggc cttttatcca ggagctgtgt ctataggcgg agagtccgat
     2881 tccggaaagg gagtttcgag cgatgtcata ggtaagctgg aaacccaaat cgaaagctcc
     2941 ggattgcagt attttcgcaa cccgtccttg aaagagggca ctcaagagcg attggactgg
     3001 cttaacgagc taatcggaga aaggccaata aaggccttcg tcaacatcgg gggggcgacg
     3061 gcgaacgtgg gcaccgaccc gatcttcttg tacctaaaac ctggggtcaa ccacatttca
     3121 cagtatgcgc ctttcgagac cggcgggctc ctttacgcgc ttgcacgaca gggtataccg
     3181 gtcatccatc tcctgtacgt gaagggattg gtcgcgcaaa acggtctccc gtgggacccg
     3241 atcccgctcc cctcggaact gtctttacaa cgtaaactgc ttggcgtttc ggaaaaagcg
     3301 gcgatggcga cgctgttcct cgcctattgg gctatcgctc ttttactacg ccttcgagga
     3361 gcccgaaccc ttcgtcaccg cctgggtctt ttcgatcgtc aacgatgaat ccacggtagc
     3421 cttcggttgg tggacggccc cgctccaaag cgcgtgccaa cccgcgcgtg cgatcatcgc
     3481 cgcgttgtcc atgcaatctt cgaccctcgg aaagaagacc gtataccccc atttggccgc
     3541 gcgacttcgc gccttctccc gtagcgcgcg gttcgcggac accccgccgg cgaagacgag
     3601 cttcctatac ccggtctgac ggccggctaa aaaggtcttt tcgacgagga tatcggtgat
     3661 cgcctcttga taagaagcgg cggcgtctga taccgaggag tcgggatgtt tcttcatatg
     3721 atagagcaaa gccgttttta acccgctaaa ggagaagtcc atagtgcctt gtttctttag
     3781 gggcaggggg aagcggaccg agtgtggatc tcccgttacg gctgccttcg atacgcttgg
     3841 gcctcccgga tagggtagcc ccaagattcg ggcacctttg tcgaaggcct ctccggcggc
     3901 gtcgtcaagt gtccccccga gatgacggag gtcttcgtag gagaccaccg cgtacagatc
     3961 ggtgtgacct ccggacacca agagtacgat gaacggaggg gcgagctccg gatgagtcag
     4021 ggcattcgag aagatgtggc cgatgagatg gttcactggg ataaaaggga tgcctaaggc
     4081 gcaggcgacc gcttttcccg tgcacactcc caccaatagg gagccgatca aacctggacc
     4141 gatcgtacac gcgaccgctg acaggtctcc ccaagagaga ccggcagatt cgagggcacg
     4201 atccaaaagc gtgtggatcg tttcgaaatg ctttcgagag gcgatttcgg gcacgacccc
     4261 gttaaacacc tgatgggcgt ctatctggga gagcaactcg ttggcgagga ccttcccctc
     4321 gtcgtccaaa atcgcaacgc ccgtatcatc gcaagaggtt tctatcccca acagatagcg
     4381 acgggctttg ggtaccgaat tagacacgaa aggtcttcgg ctccgtacgg gcgccaaata
     4441 tcgccgttcc gatgcgtaag atcgtggcac cttctcccaa cgcgatctgg aaatcgttgg
     4501 acatacccat cgataggtgc tttaattccg gatacgactt ttgataggtg tcccgcagcg
     4561 tccgcagcgt ggaaaaggat tttttaagaa ccttctcgtt cgaggtcaac ggagccatcg
     4621 tcattaaccc cgttatacga atatgctggc aagaacgcgt ggcttccaac aactcgggca
     4681 agagcgccgg agatacgccc atcttgttcc actctccgga ggtgttcacc tcgatcaaaa
     4741 tatcctgagt gagggtgcgc ttcctgcaga actcgtcaat gcccagcgcg gtttctgcgg
     4801 aatcgatcga atgaattagg gccgttttac ccaccacgta tttaaactta tttttttgga
     4861 gagcgccaac gagatgccag gagattccct cctccttaat ctccaagaat cggtcacgga
     4921 gtgattgtgg ccgattttcg ccgaattctc gatggccgca ggcgtaggcc gccacgaccg
     4981 cttccggcgg gaaggttttg ctcacagcca ccaacgtgat gtcagaggtg gggcgcttgt
     5041 gtttgtcgca aatcttcgcg atctccgttt ttagccgctc taggttctct ttgatatgct
     5101 ccatttggtc aggctccttc gcgacgcgtc ctttctagac gaaagacacc cttgagggcg
     5161 tctttcgtat tgattgatag tgtttacagg ttcaggaccc agagtccgtg aatattgcag
     5221 tactccctca cttgcttgat ttgacatccg cagtcgaaag tcacctctgg cgctcccccc
     5281 ggtttcaggt acttgcgatg gaccccgtga tcggttacca gctcgatcca ttcgatatag
     5341 tgtttctcta tcatcggatg caatgtggaa ccgacgacga ctttgacgcc tttttcgagc
     5401 ttctcgagca ccggaatgtg tttctctttc gcttgctcgg ccgtctgctc caccatcagc
     5461 ttcattggtt ctccgcagca gaccagcgtg ccggcgccgc tatggacgac ttccacgatg
     5521 ttgccgcact tttcgcattt atagacctgt tctttggctg tcatccggaa cctcctttta
     5581 cgtgtgatgt ttttttacga tgagatcgac caagcgatcc atcgcttcgt agtcttttgc
     5641 ctttgggaag cccttgatcg tgatcggatc cacccactcg gcctttaaat tgcccaatgc
     5701 gccttttgcc gcttccacga ccttggtgcc ccacccgtaa gaggtgacga gtgacagata
     5761 cttcgttttc ggctttaacg cattggtcaa caccatcgta tacagcatct gcggatgcgg
     5821 gcctagaagc atagtcggtg tggcgaacag aagggtgctc gcatcgacga tatcgatcgc
     5881 aagttcacca agatccgaaa cggacatatt cagagggatg acctccatct ttttctgtac
     5941 aaaggcattg acgagatggt cgacgatagc ttccgtgctc ccgtgcatag aaatgtaggg
     6001 tatcagaact cgtggttcca cgtcatcgga aacccaactt tcgtacgcgg agaggatgaa
     6061 gttcggattc ttgtagacgg gcccgtgact gggacatatg aggaccgggt cgagacctct
     6121 cacggtcgca aggtgttttt ggacaaaact ccggaagggc atcatgattt cggcatagta
     6181 ccgcttcgcc gctcgataga tcctctggct gtcatcggag tagacttcgg aggtggcgta
     6241 atgggaaccc agaaaatcgc acgggaacaa aatgcggtct tccaccaaaa acgttaacat
     6301 cgtctccggc caatggaccc acggagcgaa aaagaaccgg agggttttgt ttcccagact
     6361 caaagtctgc ccatccgaga cgacaatgaa gtcctcggtt ttcagcggta gatgttccag
     6421 caaaatctct ttgcacttct cgttcgtgac aactttcgct ccgggatatt tcttaagcac
     6481 cagcgggatc aacccggaat ggtcttgctc tgcatgattg gcgatgacgt agtccacttt
     6541 tttcaccgga aggccttcca atccgcgtct gaactcttcc ggcttagaag gatcggaggt
     6601 atcgatcaac gcagtcttct ccgatccgac aactaagtac gcgttataac tggtgccgtc
     6661 cggtagcggg ataagttcgt cgaaaagccg gcgcgaccag tcaataactc cgatgttgta
     6721 gatggtgtca gaaatttttt taatagccat agcgcacctc caagggtagc ttcattcttt
     6781 ttcgaaggcg tcttttccgg cgccacattc ggggcaaatc caatcgtctg gaatatcctg
     6841 aaagggagtg ccagccgata tccctcctgc cgggtcaccg atctcggggt catagacgta
     6901 accgcatatc gtgcatacgt atcgttccat agtctctacc tcctttttta gctggttgga
     6961 tagattatac tacaaaaaac ccggggcatt tccgtcgcgc tcaatggtcg cccggactac
     7021 ctcgctctct agacgaaaaa taccggctaa aaaaaccacc tcatccgtat aagagtgatc
     7081 ccccctctct gtcggttttc gcgagggaac ggacggcatc actccgattc gagtaccgcc
     7141 cgcgccacct gatccgccac cccagggtga aagggtttcg ggagtataga atcttccctc
     7201 aagtcaccgg gggcgatcat cgaagcgatc gcctttgcgg ccgccagctt catcggctct
     7261 gaaatttgct ttctggcttt caacgcgcct ttaaaaatcc caggaaagac cagcacgttg
     7321 ttgacctggt tcgggtagtc cgatctgccc gaaccgacca cgcgggcgcc tcccgctttc
     7381 gccagatcgg gcataatctc aggaacgggg ttagccaagg cgaatacgat cgcgttcgaa
     7441 ttcatcgagg cgaccatctc ggtggtgacg atattaggac cggacacgcc gataaacaca
     7501 tcggcccccc tcatcgcgtc ggccaaggtt cccgtcttct gtacaaggtt cgtgaactcg
     7561 gccaactctc tttgcgccca attcaaccac ggcgcctccc gatggatgat accggctcgc
     7621 acacaaaccg taatgtcttt gaccccgtag gttttcagga ggaaggcgac cgcgttaccg
     7681 gcagcccccg cgccgctgat gacgaccttg acgtcttcgg ctttcttcga cacgaccgtc
     7741 agcgcgttga tcagcgcggc taaaacgacg atcgcggtgc cgtgttgatc gtcgtggaac
     7801 accggtatcg acaggcgaga ttttaacgtt ctctcgattt cgaaacaacg cggcgcgctg
     7861 atatcttcca agttgatccc gccaaatacc ggggcgatcc attctaccgc ttgaacgatt
     7921 tcctcgacct ttttcgtgtt caggcagatc gggaaagcgt cgactccggc gaattctttg
     7981 aacagaaccg cttttccttc cataaccggc aacgcggctt ccgggccgat atcgcccaag
     8041 cccaacaccg ccgtgccgtc ggtaacgacg gccacgcaat tgcccttcat cgtgtaacga
     8101 tagaccgcct ctcggtcttt gtgaatctct ttgcagggct cagcgactcc cggcgtgtag
     8161 gcgatagaaa gatcgtccat cgaacgaacc gacgctttcg actccacact tagcttccct
     8221 ttccacctct cgtgctcgac caacgccctt tgtagcaagt ccatcccgat cacctctttt
     8281 cccaagggag cggcaggtcg gaaaggccgt aggtttcttt caagccgctc atctgctttt
     8341 ggagcgtcgt gttcactgga acgcccttgt cctttctttc cagccaagcg aggtgttctt
     8401 tttcgccggc ggtatagatg cgatccgctc cgggcgcttt tttagaagct cgcagttccc
     8461 tcaaaatcgt gccggtgata cgtttaaact cttccaactc gatgaagctc tccacgttga
     8521 tggcgataaa aaagtgcccg acaccgaaag gccgcttgtt tccgagctcg tcgaaaccgt
     8581 tcagcatctt gaggaagtac ccgttttgta gagaagcgga caagatttcg accaccgtcg
     8641 catagccgta ccctttatag cccgctagcg cttctcccgg gccacccagc gggagcaacg
     8701 ccgcttgacc ggatacgagg tccgataaaa tctgatgcgt gtcggtcctt atacgtccgt
     8761 cgtggccgat gacccatccc tccggaatgt cttttccggc gcggtcgtgg acctcgatct
     8821 ttcccctttg cgtaatggag gtcgcgcaat cgagaaggaa cggaaagtct tcgtccgtcg
     8881 gcatcccgaa ggtcaaagga ttggtgccca acatgttctc cacgccgaag gtcggcgcga
     8941 tagagggtcg cgcatttgtg ccagtgatgc cgatcatccc tctttcggtc gccatcagcg
     9001 gatagtaacc ggcgatgccg tagtggttgg aatggcgcgt cgccaccata ccgataccgt
     9061 agaccgccgc tttcttgatc gcgagttcca tcgacgcttt cgccaccacg aaacccatac
     9121 cgttgtgccc gtccactacg gcggtggctg ggctctctcg gacgatctct gttttggtca
     9181 ccggttgtaa ggtcccctct ctgatcctat cgatgtaaat aggttttaaa cggccgatac
     9241 cgtgggattc tataccccgt ttatcggact cgagcagtat ctccgtacac gtcttcgcgt
     9301 cctcgtttgg aatgccagcc gcgcaaaaaa ccgcctctac gaagacttta gcgtcttcga
     9361 aaggccacca aaccacacga tcatccagca tatcgattcc tcctttgtta aaagccgatc
     9421 acgaaacgcc tgatgctttt gggaagaaag tcgctcaatt tgatgatacc ccaacagaat
     9481 agtataccaa aaaagatcat cagtaagaat tctacggaac gggaaaaacg gtagcctatg
     9541 gcttcttcga accagacgag gaagaaccac atgaacggtt ggtgggtcaa gaacagcacg
     9601 aaagaaaagc gaccgaagcg gataaaaatg gaaagaaccc ctacgccaag cttttctcta
     9661 agaggcctag ggagggtctc ttttctagcg cgaagggaat gccaccgttt ttccaggcga
     9721 tggctgacgg agccaataca ggaaagcgcg aagatcgaga ggaaaaaagt gaatactgaa
     9781 aactgatcga tcacgaagta ggtctgccat ctccgtacaa agagaaaggc ctccgtcccc
     9841 gtgagggcca ggagaatgag gtacccgtac acccctctct tgccaaagga aggaaagacc
     9901 ggcttcctgc tggcacttct caagtacccc caaaaaaacg gaaagaacca tagcagcgga
     9961 ttcggataca aaagggcgcc gagtgagagg agcggaatct tttcctgtgg aatcacccac
    10021 aagctaaccg aaaggatggc taaaaccgtt gtctgaaata taaaagcacg tctgagaagc
    10081 ttaggataca gttcagggtg gacattacgc agaatacccg tgaagtattg gaacaggatg
    10141 agaataaata gaaaatagta ttgccaagac cccgaaaagg tcaaaatgct taagacaaac
    10201 gagagcgggg tgatttcgta gccgaaaatc tctcggccgg tatacgggta ggtcacacgc
    10261 gaatcggaca ccgtgtagat gagaagtgag agcgtggccc aaaataggta gggtttgacc
    10321 atatcgacga ttctttccag attcatacga gtggtcaccc gcttgcgttt cagcccctgt
    10381 ccgtagccaa acagatagat cagacagaaa acggccggaa ccatcgattt gccaaggtaa
    10441 gagaagagca gctgttctag tacgggcatg tcgttgatca ccgcggccaa agaatgaccg
    10501 gcgataacag aaaagatcga tatccctttc gcgatctcca aatagactgg actaaggctc
    10561 aaggacgctc accacacgaa tccaccaggt gttgcgctat ttcgccgtgg tttcggtttt
    10621 gacgtatcgg atccctaggc gttggtcttt cggtaagaac aggatcagga taaataagag
    10681 gattccgaac tgaaaaagca tcatcaagat gtaggcacac gacgagaaga aggccactga
    10741 gagttttttg gaagcgatgt acaggttgga gcttagagaa aaagccaaca cgcagaaggc
    10801 caagacaagg gtgcaaagga aattgaagga gtaaagggac agtaacccga agatcaggtt
    10861 ggcggacaac aaaaccgcca gtaagatgac gggaaagtct tgctcaaagt attggttcat
    10921 cgcgtacttg ttccatttcc atctctgctt caagagcaag aaaagggttt gtggcgcgcg
    10981 ctcccaagtg tggcaactga tcgaaaagcc ttttttacgg gcaaaggcgt tcgcttgccg
    11041 gcctatttcg gagtcttcac tgtacacttg agaaggaaag ttaatcttgc tcgcgacctt
    11101 ggaagagatg aagaaggagg agcccgtgaa gttgaacgga atcatattca gcaactgcaa
    11161 accggacacg aatatctcgt ccgcgtcggc gatcgagtag ctgagcgtac cgtgttcaac
    11221 gtttcggcgg gtcaaatgca aaacatcgta cccttctcgg agcgtgtaac ccaaggcttt
    11281 cgaaaaggtt tccttcgaga ttgccgtatc ggcatctccg acgaagaata gatcgcattc
    11341 gatcgtgtgg aaagcctctt cgagagcgat acctttcgga tgtatccgtt tctcagaagc
    11401 gggaacggag cggatgtgca gacggtctcg atagtctttg ccggtgaccg gatcgatctc
    11461 gcgttgcagc gcgcgtaaca tctcgacagt ctgatcctcg gagtgatcgt cgtaaacggt
    11521 gacctctatc gggccggcca agcttaggta gtcgcgaacg ttttcgatga atcgttcccc
    11581 ttcgtttttg accacgaaga gcacggcgat acggggttcg tgaaaaagcg actcctcgta
    11641 gggcagggct tcaagcgtca tttgctcgag aaatcgtttc cgcgaagtat agaataccaa
    11701 aaaaaaggcg ccgacgctgg ctaacaaaca cggaaacaca tagcttccga aagaaaaaat
    11761 acggctaaac gcataaacca aggggtagac cgcgatgatc gaaatccatt taaaccaaaa
    11821 catagcgcct ccctctatac agtggttcgg cccgggcgca attcttcata aagcgccttc
    11881 cattgcttat agatcgctgt ttctgagtaa atcgaagcga gccgtcgcgc gtcctcgatc
    11941 cctttccgcc gcgcggcctc gtcttggcag atacgtcgga tccaatcaga aaaactttgc
    12001 acatccgtcg cacgataata atggccgaag aggatacccg ggtagagggg aatatcgcgc
    12061 aacagaatag gtaacccgca ttttgccgcc tccaagaggg acatcgggaa cagctcctga
    12121 aaagaggcca ggaagaagag gtcagaggcc tgcaagtatt cggcgaccac ttcccggtca
    12181 acgattccgg gaaagcgcat attttgagga ggatcggcca cgatggcccg ataggtgtcg
    12241 tagccgtcgg acaaccgtcc gaaagaaaac ccccctaccc aaacgaaaag atagtccgga
    12301 ttcaactgtg cgcaggcgac gaaatcgtca aatccctttc tccgttgtat ctgccccgaa
    12361 gagacgatca ccggagcctt gagcgggagg tcaagctcgc gtcgcaaggc cgcctttttc
    12421 tcctcattga cctcaaaaaa gaggcgctcg tcagcatagt taggaatgaa cgatactttt
    12481 gccggatcca accctttgct gatcagctct tcccggtaat acgggttgac aacaaccaat
    12541 cggtcccctt gccggtagaa atagtacaag tatttaccga aatactttac gacgaatttc
    12601 ggcaggctga ggctcccatc gagtgagtct tccatcatat ggcaggtgat gaccacgcct
    12661 ttcccccgtt tatgaaaatg gcggatcaac cgcagggatt tcaaatccac cgtgtgcaag
    12721 tgaaggatat cggttagaga atcggtgttt ttcaggactt ggaattcgcc gccccgctcc
    12781 aacaagcgca ccagatcatt gtaagcggag tcaactccgt gccccttttt tgttccggat
    12841 atcgaacgca tattgacttg caactggtaa gacccccaca tcggatattc tagctacttt
    12901 aggttttttt tcaaaggatt ataacacgaa accgacctgc tcgagctgcc tgtgcgtgtc
    12961 aatttatgag gtgccgccaa gaaacaaggc cagaatcgac cgcacgattt ttagtccgtg
    13021 taatcgtcac ggcgtaaacg ccccctgttt gcaagaccgc gcgatacgct tgagaacgcc
    13081 ttgggaacgc caacttttga gttggcatat cttgaaaaaa agtcacacag aggcctcgga
    13141 gagtacaaag aagagaggta tatacgagag ccaacaccag gttgatgttt tctctgcgta
    13201 ctctgtgcga aaccatcttt ttttgaatac gccgaactaa agttcggcgt tcacaaataa
    13261 gacgggcatt ctacgggtgt caatctccga ggacagaaaa ataaccgtga tagagtaaaa
    13321 agaagcacag gtaaaagacc cgcgcctttc ggacgtacgc ggttttctgc cacccgccgg
    13381 cctccagcag ggctcgaatg taggtggggc cttgatgata ggcagtgaga acgatatccc
    13441 aatcctcttc gaacatatcg gccaggtacc gaagataacg ggtacccagt tcgatgttgt
    13501 tgtgaggttc gcgccaactc cccacgatcg ggaggctctc ttcgagccac gcggcggttt
    13561 ggggcagaag ctgcatcaag ccaaatgcct tcgccggtga ttgcgctaac gagtcaaacc
    13621 cgctttcggt ttggatcacg gcggccgtca acaacgggtc gatgccgtac ttaccactgc
    13681 tgttgacaat ttggggcagg tatggtagct taaacaccga ataaccgatg cacaacaggg
    13741 cgaatgcgac gatcggcaag accaacaaag agaagagaag gaagggcgac cctttcaacg
    13801 ggaggacctc ctttttattg gaaggtcg
//
LOCUS       DAOPXN010000054        13699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4574_length_13699_cov_5.882659, whole genome shotgun sequence.
ACCESSION   DAOPXN010000054 DAOPXN010000000
VERSION     DAOPXN010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13699
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4574_length_13699_cov_5.882659"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(554..1858)
                     /locus_tag="PKM49_04500"
     CDS             complement(554..1858)
                     /locus_tag="PKM49_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNY82108.1"
                     /translation="MKKREQCILSPEEIPYYWYNVKADLPFTLDPILHPGTNQPAKPE
                     DLKPIFPDGLIEQEFTLQREVPIPEAIRDEYAVYRPSPLVHATRLEEYLDTPAKIYFK
                     DESVSPCGSHKVNTALAQAYFNKREGIEKLVTETGAGQWGSALAYAGKKFGLTVEVYM
                     VRVSYLTKPARQQMMHLFGAEVYPSPSERTEHGRAVLETDPNCNGSLGMAITDAVEVT
                     VKSKNAKYSLGSVLDHVLLHQTVIGQEVKKQLKKMNVKPDILVACVGGGSNFGGFVLP
                     FIPDRLKDPHLEIIASEPEACPSLMKGEFRYDFGDTGHLTPLLKMCTLGSDFLPPPIH
                     AGGLRYHGASPIVSRLVAEKYVRPDDLAQDEILQAAQIFSLTEGIIPAPESAHAIATV
                     IKEAKKCKANREPKTIVFNLSGHGHLDLASYDFIAKINGNSK"
     gene            2422..3798
                     /locus_tag="PKM49_04505"
     CDS             2422..3798
                     /locus_tag="PKM49_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82109.1"
                     /translation="MRKKAYYLVLWMLLVLFATTLLGDPDVVITQVTIPTTLPLSQPV
                     VGTLTTAPTFQTEPVPVPNEPALPMISVEPEWAIFPPFDIDFELLTAFRTSNGFFSQD
                     GFQLRIQQNLLPLSAIGTLFSLNGATPTLLKFGADIEKVLSGDVNLSAIGNTDFHFGH
                     TVDHFGIGVFVKDDLDSRLFLPAWFAQTLAGKIPPANHAEDFSGSVIDVHLSAGVFAA
                     LKFDYFQVAGEIGMYTPVLTSHFNTGSNLVSNGTTYAGTATVTGQLYSSADLGALDLF
                     TLPHALLTSGGWKADLSILIGKEKTLAGFSVSNIPITDAKLNFIAPWSLNYQASSGSA
                     TPTLTTTEALIWTPAEHTYRIKTTFAGFINVPMAEVGFFQLHGKWRPLSGTDIGGMLH
                     FFITDGFSLMGDFTYNTTGFYYYSVGLGFYGSKTRTNVDIGIGSRLFFDYATLTSPRI
                     SVSSSANY"
     gene            3935..4126
                     /gene="rpmB"
                     /locus_tag="PKM49_04510"
     CDS             3935..4126
                     /gene="rpmB"
                     /locus_tag="PKM49_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNY82110.1"
                     /translation="MAWICAVCGKKPSTGNRVSHSHRKTKRRWKPNLQNVTVGSETGN
                     KRIKVCTKCLKAGKVKKIA"
     gene            4189..4524
                     /locus_tag="PKM49_04515"
     CDS             4189..4524
                     /locus_tag="PKM49_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82111.1"
                     /translation="MVFILFFVADCLLLTAFLFFPAWIGLKAAFLLYAVGVGILVHRF
                     LSATLVCDPLEGSILYRKQGYRFERVLFSKKRFGNHLLFMVRLRDDKVVRLSLRPYDL
                     ASVKEIFSL"
     gene            4544..4756
                     /locus_tag="PKM49_04520"
     CDS             4544..4756
                     /locus_tag="PKM49_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY82112.1"
                     /translation="MPEKKRYNVRINPEFCKSCGICYTVCPTKTIKKGTANAPKVDDP
                     DACIGCKQCERLCPEFAIDVSEREGE"
     gene            4761..5918
                     /locus_tag="PKM49_04525"
     CDS             4761..5918
                     /locus_tag="PKM49_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNY82113.1"
                     /translation="MIKTGEYVLVSGDEACAYGAISAGCRFFAGYPITPATEIAETFA
                     QELPRVGGTFIQMEDELASACAIIGASLAGVKSMTATSGPGFSLMQEAIGYAAMTETP
                     SVFVNVMRGGPSTGMPTKVSQGDIMQARWGTHGDHPVIALYPNSVKETYTLIVRAFFL
                     AERFRVPVIFLMDESLGHLRESIEVPPIPSEWILQRLADDLGDEEIYHPYEEVSGFIT
                     PLRMMGKSRFHVSGLVHDETGFPLGTSDVADKLAKRLEKKIQFHLDEIINYEEFMVED
                     AEILLIAYGSTSRSARQAVRMARVDGIKVGLFRPITIWPFPNARLKKLISKVDAALVA
                     ELNMGQIYKEVSQLNKKGKLLNLINRVDGELISPDQILQAINELRIQLDEY"
     gene            5946..7349
                     /locus_tag="PKM49_04530"
     CDS             5946..7349
                     /locus_tag="PKM49_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNY82114.1"
                     /translation="MERRVEIIIDAQKMNAFLVFHPPDGDTRGPERSEIDEVVQAQNI
                     RFGIMEEKINAFLSTYIPGVPHPFARGILAQPCQDADIVFNFDMEQLKAFCSGKLSQS
                     DKNLYRNFIVKKGTLIAKKIPAVEGKPGTDIFGSEVLPKAPKDKSLLIYKGKNTALID
                     NGLRLVSEGEGILQLEGERINVDNVLFVRGNVDVGIGNIEFEGSVIVEGMVMPGMVIK
                     ARDDIKVSDIVEGATLIAGRDIEIEAGVKGRSKTFISAGRDAKVKFIENAELEAKRDI
                     QISNAAVNSTVRSKRNVIVVGDPGSIIGGSVSAGHAVEANEIGSDMNLKTSVEVGIDP
                     DMRQRAALLRSQIALNMDNLNKLTQIVKKLRELRNTLGDKFPPEKIEYLVKSINAINN
                     LNAETPGLQAELENILGKIAESAVGSKIVARKLMKPGTEVTIRDHKFYATQAYEKVML
                     VLEDGEIRLGGYRPEGP"
     gene            7398..7487
                     /locus_tag="PKM49_04535"
     tRNA            7398..7487
                     /locus_tag="PKM49_04535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7433..7435,aa:Ser,seq:cga)
     gene            7526..7903
                     /gene="yajC"
                     /locus_tag="PKM49_04540"
     CDS             7526..7903
                     /gene="yajC"
                     /locus_tag="PKM49_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNY82115.1"
                     /translation="MEFLLRAFAAPDTAAGAGADSAAAPAGGLGSFGLIIWIVVLFAM
                     FYFLIIMPQRKRDKQYKTMMNELKVGDKIVTIGGIIGKVVSLKEQTVDIQTADTRLEI
                     TRRSISAILGKSTKDPNEAVQRQ"
     gene            7944..9353
                     /gene="secD"
                     /locus_tag="PKM49_04545"
     CDS             7944..9353
                     /gene="secD"
                     /locus_tag="PKM49_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNY82116.1"
                     /translation="MKADRIRLFFVVVILIVALASIFWPNKSEAFLDSSINIFFQNIR
                     LGLDIRGGTRLDYRVVVPKDIERSAGTIADEVVVVVRQRLDAANYTEAVVSKIGGEDA
                     RIRVEIPGIDDPTIAERLVGKKGQLYFGEILEQQTAATMPDKRIGIKYANALWVPSKD
                     KTPNNERVWYLLNPYVTVGSQKLYLGGSEIKNAKASVDTQKGGFKIDLEFTAKGTELF
                     GKITAAFVEQQLPIVLDDVVLVAPQVMSAIRSSNAEISGRFTAQEAMELAALIKSGNL
                     PADLQKLEERTLGPTLGRDIITVSLIAGMFGLLIVLVYMIVFYGLSGFIADLALIYNT
                     VLILGAMALGKFILTLPGIGGIILTFGTAIDGNIIIIERIKEEIRAGKTVLNAITAGY
                     SRSFSAIFDSNVTSILAGVVLYYLGTGTIKGFATTLIIGILGSMFATLVVSRVLTNMV
                     SSKLHFRVSKEIAKAGDVK"
     gene            9350..10252
                     /gene="secF"
                     /locus_tag="PKM49_04550"
     CDS             9350..10252
                     /gene="secF"
                     /locus_tag="PKM49_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNY82117.1"
                     /translation="MSIKTFDFCGKKKIFFAISLTLIAISLISIFTRGFNFGVDFSGG
                     TELIFKSNRDMTVAAFREQIGGIEKALAAANISKLSQMGDQEQNEYRYSVTTVLFYES
                     DQKQQLEGLARESGLEIESYNSVSGFAAEELRSKAIWIVIFVSAILLVYITFRFRYEF
                     GIGAILALLQDAVITLGFYSLFQIPFDVSVVAAILTLLGYSINATIVIYDRVRENMRL
                     MRGKGIAEIINTSIWQTMTRTVHTSMTTFIVVFFLLVFGGISLRPFAFGLTVGVVAGT
                     YSSVFIASPLISGFLQGKKKKSYS"
     gene            complement(10501..11778)
                     /gene="serS"
                     /locus_tag="PKM49_04555"
     CDS             complement(10501..11778)
                     /gene="serS"
                     /locus_tag="PKM49_04555"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNY82118.1"
                     /translation="MIDIKKIRKEPDHIKEALSIRNDNPDLVDQILALDAQRREWVAQ
                     TDALKAERNTLSQLVAKQKAENKSADVSHWVEKSKAIGDQIKANDERLAQLESQQQAI
                     VSLLPNIPSDKVPRGTDENHNVEVRRWGEPRVFDFEPLPHWDLGSNLNMMDFERASKL
                     SGSRFVVLKDKVATLNRALISFMLDVHTREHGYVEVNVPHLVKRESMFATGQLPKFEQ
                     EAYRTDPDDFFLIPTAEIFLAGMHRDEILSEKELPLKYVAYSSCYRREAGSYGKDVRG
                     MIRVHQFEKVELVKYTTPETSFDELEKLTADAERILQLLELPYRVVLLCTGDLGFGAA
                     LTYDLEVWLPSYQKYREVSSCSNDTDFQARRGNMRYRNAQNKMEFVHTLNGSGLAVGR
                     TLIAVLENYQNPDGSITVPKRLIPYMNSIEIIP"
     gene            complement(11864..12460)
                     /locus_tag="PKM49_04560"
     CDS             complement(11864..12460)
                     /locus_tag="PKM49_04560"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNY82119.1"
                     /translation="MDWRGFIDSEVFHWVVLPALIFSARVLDVSMGTLRIILIARGRR
                     AIVPIIGFVEVFIWISVMSQLMARVDNPFYMVAYAAGFATGNYLGMVIENKLAIGKAC
                     IRMISGNDLLPLVNALRQAGFGVTVADGWGSKGPVKILYTIVRRKEIAMAVQIIHRFE
                     PKAFYTVEDLRDAKEGVFLREARMEKSPNQLWLRKKEK"
     gene            13010..>13699
                     /locus_tag="PKM49_04565"
     CDS             13010..>13699
                     /locus_tag="PKM49_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HNY82120.1"
                     /translation="MVCVLFFILCLSFAFGHPQIEYRLSRLGYEDGADLYGVHSRIAF
                     FFPRPSIPTDTALTIRLRYAYTDLAGPKTMVTFLVNGRPIALRTLPNRVGELTVSLPM
                     EWVPGWQGLTVEALVTLDYSECDNKPLAKESLWFSIEPETTFSFTGNHAPPKTIVDFF
                     RAGGYASPLRLALTEYTSSSLKSLCQTSLLLGYLSKGLKKEVSLVSSLPDAGHGVLFK
                     PGAGIRLNGNTL"
BASE COUNT         3195 a         3516 c         3680 g         3308 t
ORIGIN      
        1 tttttgagaa agtggctttt atcgccgttt gtagcacttt tagtcgaaag ttgggggatg
       61 gtcaccgagc ttcaaataat gtatcgaaga gccaaaaaac aaggccagaa atcgatcgcg
      121 cgatttttag tccacgtagt catcgtggcg tagaaggggc aaagggcgga tcgcggggtc
      181 gtaaaaacca cttaccccgc gataacacac acaatcatcg cggcataagc tcccctactt
      241 ttcgcggggg cgcgatacgc tttagtgcgg catggctttt tcttttcact gggaacgcgg
      301 aacttccgtt ccgcctgcgt tctatggtct ctccgtgccc tctgtgtgaa accttctttt
      361 gtctttttaa aggtatgaag acataccgaa ctcaagttcg gcatgttctt aagagtagaa
      421 gataatagag aggattgctg gacttcgtta gtatcgcggg gtcccgttgg ttaccccgcg
      481 ataatcttcg tacactaatc cgtctccact cgaaaaagtc acaaaagtgc agggcgagcc
      541 ggaaggttgt gggttacttg ctgttaccgt tgattttggc gatgaaatcg tatgacgcca
      601 aatccaggtg cccgtggccg gagaggttga agacgatcgt cttgggctca cgattcgcct
      661 tgcacttttt cgcttccttg atcacggtgg cgatggcgtg ggcggactcg ggcgcaggga
      721 tgatcccttc ggtcagagag aagatctggg cggcttggag gatctcgtct tgagccaaat
      781 catcgggccg aacgtatttt tctgcgacca agcggctgac gatcgggctc gcgccgtggt
      841 agcgaagtcc gccagcgtgg ataggcggcg gcaagaagtc ggaaccgagc gtgcacattt
      901 tcaagagagg ggtcaggtgg ccggtgtcgc cgaaatcgta ccggaattcg cccttcatca
      961 gagacgggca ggcctccggt tcggaagcga tgatctccaa atgggggtct ttcaaccggt
     1021 ccggaatgaa ggggagcacg aatccgccga agttcgatcc cccgcccacg caagcgacca
     1081 ggatatcggg cttgacgttc attttcttga gctgtttctt gacctcttgg ccgatgacgg
     1141 tttgatggag cagcacgtgg tccaagacgc ttccgaggga gtatttggcg ttcttgctct
     1201 tgacggtcac ctctaccgcg tcggtgatcg ccatgcccaa agagccgttg cagttggggt
     1261 ctgtttccaa gacggcgcgg ccgtgctcgg ttctctcact cgggctcggg tagacttcgg
     1321 ccccaaaaag gtgcatcatc tgttgtcgcg ctggcttcgt cagatagctc acccggacca
     1381 tatagacctc taccgtcaaa ccgaactttt ttcccgcata ggcgagcgcg cttccccatt
     1441 gccccgcccc ggtttccgtc acgagctttt cgatcccttc cctcttgttg aagtaagcct
     1501 gcgcgagtgc ggtgttcact ttgtggctcc cacacggact cacgctttcg tctttgaaat
     1561 agattttcgc aggagtgtcc aggtactctt ccaaccgcgt cgcgtgaacg agcggagacg
     1621 gacgatagac ggcgtattcg tcgcggatcg cctccggaat cggtacctct cgctgaaggg
     1681 tgaactcctg ttcgatcaga ccgtccggga aaatgggctt gaggtcttcc ggcttggccg
     1741 gctgattggt gccgggatgc aaaatgggat ccagtgtaaa gggaagatcc gctttcacgt
     1801 tataccagta gtatggaatc tcctccggtg acaagatgca ctgctctctc tttttcatac
     1861 tccataaccc ccttctattt ggtttgagtt tttttcaaaa aaaaaagcgg ggaactcttc
     1921 gttccccgct tcagacttat ctgaaccctt ttaagcagcc aaagagctcc cgtccgaaac
     1981 ggggagtctc caccaccaaa tggacttaca ggatagggcc gctttacagg gcgtcgcgtt
     2041 catcgttttc ctccgaatga aatttcccct attgtaccag aaaaaacgag cgatgtcaaa
     2101 gaaacgattt gtccgtcttt ttttgaaata ccggcgcgca tcacgtccga gagggatcga
     2161 cgcatactca gctcatagag cggttttatg gagtccgtgc ttcggaatta tcggttattg
     2221 actcagcctc tcgtgtgaaa aaagccccgt tgatttctgc gcattggcgc ggctcaaaga
     2281 aatagagatg tttggtctat aatgaaagag cgaagggttt gccatttcta cgaagtttgt
     2341 ggtaaaatga tagaagtcgc gaagtcgatt cgcgaggttt ttgcttattc aacaggggta
     2401 tggatgataa gggggaggaa gatgcggaaa aaagcgtatt acctcgtact ttggatgtta
     2461 ttggtgttat tcgctacgac gctcttgggt gaccccgacg tggttatcac ccaggtgacg
     2521 atacccacga ccttaccgct ttcgcaaccg gtcgttggaa cgctgaccac ggctccgacc
     2581 tttcagactg agcccgttcc ggttccgaac gagcctgcgc tgccgatgat ctccgtagaa
     2641 cctgaatggg cgatattccc tcctttcgat atcgatttcg agctgttaac cgcctttcgg
     2701 acctctaacg gcttcttctc gcaagacggc tttcagcttc gtatccaaca gaacctctta
     2761 ccgttgtccg ctatcgggac gcttttctca ctcaacgggg cgacaccgac gctcttaaaa
     2821 ttcggagccg atatagaaaa ggtcctctcc ggtgacgtca acctttcggc gatcggtaat
     2881 accgattttc actttggtca taccgtcgat cacttcggta tcggcgtgtt cgtaaaagac
     2941 gatctcgact cccggctgtt tctgcccgca tggttcgctc aaaccctcgc gggaaagata
     3001 ccccccgcga accatgccga agatttttcg ggcagcgtaa tcgacgtcca cctttccgcg
     3061 ggagtttttg cggcgttgaa gttcgattac tttcaggtgg ccggggagat cgggatgtac
     3121 acgccggtct tgacctcgca tttcaatacc ggatcgaacc tggtgagcaa tggcacgacc
     3181 tacgccggaa cggccaccgt gaccggccaa ctgtattcgt ccgccgatct cggagccctc
     3241 gatttgttca cgttaccgca tgcgctttta accagcgggg gttggaaggc cgacctgtcc
     3301 attctgatag gaaaagaaaa gacgctcgca ggcttttccg tctcgaacat tccgattacc
     3361 gacgcgaaac tgaacttcat cgctccttgg agccttaact accaggccag ttccggatcc
     3421 gcaacgccca cgttgaccac gacggaagcc ttaatctgga cgccggcaga gcatacctat
     3481 aggataaaaa ccacctttgc cggttttatc aacgtgccta tggctgaggt tgggtttttc
     3541 caactacacg ggaaatggcg ccctctatcg ggcacggata tcggcggaat gctccatttt
     3601 ttcatcaccg acgggttctc tctgatgggc gattttacct ataataccac gggtttctac
     3661 tactactccg tggggcttgg tttttacgga agcaagacgc ggacgaacgt cgatatcggt
     3721 atcggctcca ggctgttttt cgattacgca acgctcacat ctcccaggat atccgtcagc
     3781 tcctctgcta actactaaac atgtgaccgt caggtatctt taccttgatc ggcccgttaa
     3841 atttcgatta ggctgcgcgg ttgcttgcaa aaaggtccgg gaattgatat aatcggcgtc
     3901 gaaaaagtat tgtctttggg aaaggaggag cgctatggcc tggatatgcg cggtctgtgg
     3961 gaagaaacca tccacaggga atagggtcag tcattcgcat cgcaaaacga agcggagatg
     4021 gaaacccaat cttcagaacg ttacggtcgg ttctgaaact gggaacaaga gaatcaaagt
     4081 ctgcaccaag tgccttaaag cgggaaaggt caagaaaatt gcataaaaag cgcggaaagg
     4141 cgcttttttt ctatgttggc tgagataccg gttaaaagat cgctaaggat ggttttcatc
     4201 cttttttttg tcgccgactg cttattgctt acggccttcc tgttctttcc cgcttggatc
     4261 gggctgaaag ccgcttttct tttgtacgcc gtcggagtcg gtatcctcgt ccaccggttt
     4321 ttgagcgcga ctctcgtctg cgatcctctg gaaggttcga tcctttatcg aaagcaaggg
     4381 taccgttttg aacgggttct tttttcaaaa aagcgatttg gcaatcattt gttgtttatg
     4441 gtaagattac gtgatgacaa agtggtgcgt ttgtcgctga gaccctatga tttagcgagc
     4501 gtcaaagaga ttttttcact ataagggtaa aggggtgtta gttttgcctg agaagaaaag
     4561 gtataacgtc cgtatcaatc ccgaattttg taaatcttgc ggtatctgtt atacggtttg
     4621 tccgacgaaa acgataaaga aaggaacggc aaacgcgccg aaggtcgacg acccggacgc
     4681 ttgcatcggc tgcaagcaat gtgaacggtt atgtcccgaa ttcgcgattg acgtgtccga
     4741 acgggaaggg gaatgagcct atgataaaaa caggagagta cgtactggta tccggagacg
     4801 aggcctgcgc gtacggggcg atcagcgccg gctgtcggtt ttttgccggt taccccatca
     4861 cgcccgcgac ggagatcgcc gagacctttg cgcaggagct tcctagagtg ggcgggacct
     4921 tcatacaaat ggaagatgaa ttagcgagcg cgtgcgccat catcggcgcg tccctcgccg
     4981 gggtgaaatc gatgacggcg acgagcggcc ccggcttttc gttgatgcag gaagccatcg
     5041 gttacgcggc gatgactgaa acacctagcg tctttgtgaa cgtcatgcgc gggggccctt
     5101 ctacggggat gccgacgaag gtttctcaag gggatattat gcaagcgcgt tggggcacgc
     5161 acggcgacca tcccgtgatc gcgctctatc ccaacagcgt caaagaaacc tataccctta
     5221 tcgttcgcgc ctttttcctg gctgagcgtt tccgcgttcc cgtcatcttt ttgatggacg
     5281 aatccctagg ccatctgaga gaaagcatcg aagttccgcc gattccttcg gaatggattc
     5341 ttcagcgttt ggctgacgac ctgggggacg aggagatcta ccatccgtac gaagaggtca
     5401 gcgggttcat cacgcccctg cgtatgatgg gaaagagccg gtttcacgtc tccggtttgg
     5461 ttcacgatga gaccggattt cccttaggca cctcggatgt cgccgataag ctggcaaaac
     5521 gcttggagaa gaagatccaa tttcatctcg atgagatcat caactacgaa gagttcatgg
     5581 tagaggatgc cgaaatcctc cttatcgctt acgggtcgac ctctcgaagc gcacggcagg
     5641 ccgtccgtat ggccagagtg gatggcatca aagtgggcct tttccgaccg attacgatat
     5701 ggccctttcc gaatgcgcgc ttgaaaaaat tgatttccaa ggtggacgcc gcgttagtcg
     5761 ccgagctgaa tatgggtcag atttacaaag aggtctctca actcaacaaa aaaggaaagc
     5821 tcctgaactt gatcaatcga gtggacgggg aactcatttc gcccgaccag atacttcagg
     5881 cgatcaacga gctgaggatc cagctggacg aatattgacc gaatccttgt taaggagagt
     5941 ttttcgtgga acgtagagtc gaaatcatca tagacgctca aaagatgaac gcgtttttgg
     6001 ttttccatcc cccagacgga gatacccgag gccctgagag gtccgaaatt gatgaggtcg
     6061 tacaagcgca aaacatccgt ttcggcatta tggaagaaaa gataaacgcg tttttatcga
     6121 cctatattcc cggcgtccct catccctttg cgcgcggcat attggcacag ccctgtcaag
     6181 acgcggatat cgtgttcaac ttcgatatgg aacaattgaa agcgttttgc tctggaaagc
     6241 tgtctcaaag cgataaaaac ctctatcgga actttatcgt gaaaaaagga acgctgatcg
     6301 caaaaaagat accggccgtg gaaggaaaac ccggtaccga catcttcggc tccgaggttc
     6361 taccgaaggc gccaaaagac aagtccctac tgatatacaa aggaaagaac acggcgctca
     6421 tcgataatgg cctccgattg gtatccgagg gagaaggcat cttacagctg gagggagagc
     6481 gaattaacgt cgacaacgtc ttgttcgtgc gcggcaatgt cgacgtcggt atcggcaaca
     6541 tcgaattcga aggctccgtc atcgtcgagg gtatggtgat gccgggaatg gtcatcaaag
     6601 cgcgagacga catcaaagtg tcggatatcg tggagggggc caccttgatc gcgggccgag
     6661 acatcgagat cgaagcgggc gtgaaaggcc ggagcaagac gttcatttca gcgggtagag
     6721 acgctaaggt caagttcatc gagaacgcgg agctagaagc gaagcgggat atccagattt
     6781 ctaacgcagc cgttaattcg acggtacgtt caaaaagaaa tgtgatcgtg gtgggagacc
     6841 ccggaagcat catcgggggc tcggtctcag cgggacacgc ggttgaggcg aacgaaatcg
     6901 gttcagacat gaacctcaag acctccgtgg aagtggggat cgatcccgat atgcggcaga
     6961 gagccgcttt gctgcgttct caaatcgcgc tcaatatgga caaccttaat aaactgaccc
     7021 agatcgtcaa gaagctgagg gaacttcgga ataccttggg tgacaagttt ccgcctgaaa
     7081 agatcgagta cttggtcaag tccattaacg ccatcaacaa cctcaacgcg gagacgccgg
     7141 gtctgcaagc ggaattggaa aatatcttag ggaagatcgc cgaatccgcg gtcggttcga
     7201 agatcgtcgc gcgaaagctc atgaaaccgg ggactgaagt caccatcagg gatcacaagt
     7261 tttacgcgac tcaggcttac gaaaaggtga tgctggtttt ggaagacgga gaaatacgct
     7321 tggggggcta tcggcccgag gggccctaag cgcgtacaaa tcggacgcaa acgtgttata
     7381 atacggggca aaaaaacgga ggcgtgttcc gaactggcta aggggccggt ctcgaaaacc
     7441 ggtgagcata tttgctcgtg tgggttcgaa tcccaccgcc tccgccatct catttttgcc
     7501 aacttataaa gaggtgatac gggttatgga gtttttatta cgagctttcg cggcgccgga
     7561 tacggcggcg ggagccggag cggattccgc tgccgccccg gcgggcggat tgggttcttt
     7621 tggcttgatt atctggatcg tcgtactatt cgcgatgttc tactttttga tcatcatgcc
     7681 ccaaagaaaa cgggataaac aatacaaaac gatgatgaac gaactgaagg ttggggacaa
     7741 aatcgtcact atcggaggaa tcataggaaa ggtcgtttcg ctgaaggaac aaacggtcga
     7801 tatacaaacc gccgacacca gattagaaat cacccgccga tcgatctccg caatccttgg
     7861 gaaatctacg aaagacccta acgaagcagt acaacgccaa taattcagtt aaattacggt
     7921 aagggaaaga gaggggaagg tctttgaaag cggacagaat acgtttgttt tttgttgtcg
     7981 tgattttgat cgtcgccctg gcgtcgatct tttggccgaa taagagcgaa gcgttcctgg
     8041 attccagcat caacattttc tttcagaaca tacggttagg cttggatatt cgtgggggaa
     8101 cccgactaga ctaccgcgtc gtggtcccga aagatataga gagaagcgcg ggtacgatcg
     8161 cggatgaagt ggtcgtcgtc gttcgccagc gtttggatgc ggccaactac accgaggcag
     8221 tcgtttccaa aatcggcggc gaagatgccc gaatacgcgt cgagataccg gggatagacg
     8281 acccgacgat cgccgaacgc ttggtcggaa agaaagggca gctctatttc ggagagattt
     8341 tggagcaaca gactgccgcc acgatgccgg acaagcgcat cggcatcaaa tatgcgaacg
     8401 cgttgtgggt tccatcgaag gataaaaccc cgaacaacga gcgagtctgg tatctcttga
     8461 acccgtacgt taccgtcggc tcgcaaaagc tttacttggg cggttcggaa atcaagaacg
     8521 ccaaggcctc cgtcgatacc caaaaaggag ggtttaagat cgatttggaa ttcaccgcga
     8581 agggcaccga actgttcgga aagatcaccg ctgccttcgt cgaacagcaa ctgccgatcg
     8641 ttctggacga tgtggtgctg gtggctccgc aggtgatgtc ggcgattcgg agttccaacg
     8701 ccgaaatctc aggtcgcttc accgctcaag aagcgatgga gttggccgca cttataaaaa
     8761 gcggtaacct gccggcggac cttcaaaagc tggaagaaag aaccttgggg ccaaccttgg
     8821 gtcgagatat catcacggtc agtttgatcg ccgggatgtt cgggctgttg atcgtcctcg
     8881 tttacatgat cgtcttctac gggctgagtg gtttcatcgc cgacctggcg ctgatctaca
     8941 acaccgtcct catcttaggc gcgatggcgc tcggtaagtt cattctcacc ttgcctggta
     9001 tcggaggtat catcctcacc tttggaacgg ctatagacgg caacatcatc atcatcgaac
     9061 ggatcaaaga ggagatacgt gccggaaaga cggtattgaa cgcgattacc gccggttact
     9121 cgagatcatt ctccgcgata ttcgactcca acgtgacctc gatcctggcg ggagtcgtcc
     9181 tctactatct gggtaccggt acgataaaag ggttcgccac caccctgatc atcggtatcc
     9241 tcggaagcat gttcgcgact ctggtcgttt ctcgcgtgct caccaatatg gtctcctcca
     9301 aactacactt tcgggtaagc aaggaaatcg cgaaagcggg tgacgtgaaa tgagtataaa
     9361 aaccttcgat ttttgcggga aaaagaagat atttttcgcc atctccctga ctctgattgc
     9421 gatatcgctg atctccatct tcacgcgggg atttaacttc ggggtggact tctctggcgg
     9481 aacggagttg atcttcaaat ccaatcggga tatgaccgtg gccgctttta gagaacagat
     9541 cggcgggata gaaaaagccc tggcggcggc caatatctct aagctctctc aaatggggga
     9601 tcaagaacaa aacgagtatc ggtattccgt tacgaccgtg ctgttctacg aatccgatca
     9661 gaagcaacag ctggaagggt tagcgcggga gagcggattg gagatcgaaa gctacaactc
     9721 cgtcagcggg ttcgcggccg aagagctccg aagcaaagcc atctggatcg tgatcttcgt
     9781 ttccgctata ttactcgttt acattacctt ccgcttccgt tatgagttcg gcatcggcgc
     9841 gatcctggcc ttgctccaag acgccgtgat caccctcggc ttctactccc tgttccagat
     9901 accgttcgac gtgtccgtgg tcgcagccat cctgaccttg ctgggttact cgatcaacgc
     9961 gacgatcgtc atctacgacc gcgtccggga gaatatgcgg ctgatgcggg gcaaaggcat
    10021 agcggagatc atcaatacga gtatttggca aacgatgacc cgaacggtgc atacgagcat
    10081 gacgaccttc atcgtcgtct tcttcctctt ggtgtttggc ggaatcagtc tgagaccctt
    10141 cgctttcggg ctcacggtcg gcgtggtcgc gggaacctac tcctcggtct tcatcgcttc
    10201 tcctctgatc tcaggttttc tccaaggcaa aaagaagaag tcctattcct gataaaaggt
    10261 atccataaaa aaaagacgct gatccagcgt cttttttttg gtccccggta ggggtctatt
    10321 gggctttcga ccgaagggca aatagaagag cacgaaaata cacccctcat aaaaaattag
    10381 gcggacgatt gtccgcttaa atgtgagggg ttcattctct ttacactatt tagactgaac
    10441 cccttcggaa aagttcaaag tgttttgcag gaaagaacga atatattggt aaacatatgg
    10501 ctacggaatt atttcgatgc tattcatata agggatcaag cgttttggta ccgtgatcga
    10561 tccatcgggg ttctgatagt tctccagaac cgcgatcaac gtacggccga cggccagacc
    10621 ggacccgttc agggtgtgga cgaactccat tttgttttgg gcgttacggt agcgcatatt
    10681 cccgcgtcgg gcctgaaaat ccgtgtcgtt gctgcacgaa gagacctcgc ggtacttctg
    10741 gtaggaggga agccagacct ccagatcgta ggtcagcgcc gccccgaaac cgaggtcgcc
    10801 ggtgcatagg agaacgacgc ggtaaggaag ctccaggagc tgtaagatcc tctccgcatc
    10861 cgcggtcagt ttttcgagct cgtcgaagga ggtttcggga gtggtgtatt tgaccaattc
    10921 cactttctcg aactgatgga cccgtatcat cccacgcaca tccttgccgt aactcccggc
    10981 ttcgcggcgg tagcaggagg agtaggccac gtatttcaag ggtagctctt tctcagaaag
    11041 gatctcgtcg cggtgcatcc cggcgaggaa aatctcggcg gtaggaatca ggaaaaaatc
    11101 gtcggggtcg gtccgatagg cctcctgttc gaacttcggt aactgcccgg ttgcgaacat
    11161 actttcgcgt ttcaccagat gcggcacgtt gacttcgaca tagccgtgtt cacgcgtgtg
    11221 cacgtccagc ataaaagaga tcaacgcccg attgagggtt gccaccttgt ccttcagcac
    11281 gacgaagcgc gatccgctga gtttggaagc gcgctcgaag tccatcatat tcaggttgga
    11341 acccagatcc caatgcggta ggggttcgaa atcgaaaacg cgcggctctc cccagcggcg
    11401 gacctcgacg ttatggtttt catccgtgcc ccggggaacc ttatccgaag gaatattcgg
    11461 gaggagagaa acgatcgcct gttgttggga ttccaactgc gcgagtcgct cgtcattggc
    11521 tttgatctgg tcgccgatgg ccttactttt ttcgacccag tgcgagacgt cggccgattt
    11581 gttttcggcc ttctgcttcg cgaccagctg ggatagcgtg ttgcgctcgg ccttcaacgc
    11641 gtcggtttga gcgacccact cccttcgttg cgcgtctaag gccaagattt ggtcaaccaa
    11701 atcggggttg tcgtttctta tggacaaggc ttctttgatg tggtccggtt cttttcttat
    11761 ttttttaata tcgatcatcc caccctctcc ttcctgtcgc ttgcgcgaaa cgcccgacaa
    11821 gttttcagat gtcgcgcgct attatagcat atcggcttgc gccttatttt tcctttttcc
    11881 gcaaccagag ttggttcgga gatttctcca tcctcgcttc cctgaggaaa acgccttctt
    11941 tggcatctcg aagatcttca acggtataga aggctttagg ctcgaagcgg tgtatgatct
    12001 gcaccgccat agcgatttct tttcttctca cgatcgtgta caagatcttc accggcccct
    12061 tgctgcccca tccgtcggct acggtcaccc cgaaacccgc ttgtctcaag gcgttcacca
    12121 agggtagcag gtcatttccg gagatcatcc ggatacaggc cttcccgatg gcgagcttgt
    12181 tttcgatgac catccccagg tagttgccgg ttgcgaaccc ggccgcatag gccaccatat
    12241 agaaggggtt gtccacacga gccatcaatt gactcatcac ggatatccag atgaagacct
    12301 cgacaaaccc aatgatgggg acgatagcgc gtcttcctcg cgcgattaaa atgatccgca
    12361 acgttcccat cgacacgtcc agtacccggg cggaaaaaat taaagcgggt aagacgaccc
    12421 agtgaaaaac ctcggagtct atgaaccctc gccaatccac agccgttctc ctctctctat
    12481 ccccttttgg gtgattataa cacaatcatt tcacactttt accggctgat gtgtgtcaag
    12541 ctatggggtg taatcaagaa acaaggccag aatcgatcgc gcgattttta gcccacatag
    12601 tcatcgcggc gtaagcgctt cttattttcg cgacggcgcg atacgttttt gagagggcat
    12661 aaggcggatc gcggggtcgt aaaaaccact taccccgcga tgaacctctt agttatgcca
    12721 ctccataact tgactcacac taccgactga tggcctttgg gaaggccgaa ctactgggaa
    12781 caccctggga aggccgaact ttagttcagc atgtcttttc tataaacaca caaaagagaa
    12841 aaaagaaagt tttgcgcgtt aaccaaaacc ccaaacccgt tcccagggga acccggcgcg
    12901 cgtggcgttc ccagccgctt ccgtctgttc attgacaaac cgtcacggaa acctgtagaa
    12961 ttggatcatt cggtacatca tagggggtgt cggtatcctg acccgtcgtt tggtatgcgt
    13021 cctctttttc atcttgtgcc tttcttttgc ctttggccat ccacagatag aataccgcct
    13081 atcgcgcttg ggttacgaag acggggcgga tctatacggc gttcattcgc gcatcgcatt
    13141 tttctttcca agaccgtcca ttccaacgga tacggcgttg actatccgcc tacgctacgc
    13201 atacaccgac ttggcgggac ccaagactat ggtcaccttt cttgtcaacg gacgccccat
    13261 cgccttgaga acgctgccta accgggtggg cgagctcacg gtctccctgc cgatggagtg
    13321 ggttcccggc tggcaaggcc tgaccgtaga ggcgctggtc acactggatt actccgagtg
    13381 tgacaacaaa ccgttggcaa aagaatccct ttggttttcg atcgagcccg aaacgacctt
    13441 ctcctttacg ggaaaccatg caccgccgaa gacgatcgtc gatttctttc gggctggagg
    13501 gtacgcgtcg ccattgcgat tggccttaac cgagtacacg tcctcgtcgt tgaaaagtct
    13561 gtgtcaaacc agcctgttgc tcgggtattt gtccaaaggg ctgaaaaaag aggtctccct
    13621 cgtttcctcc ctacctgacg cgggacacgg ggtattgttc aaaccggggg cgggaatacg
    13681 gttgaacggc aacacgctg
//
LOCUS       DAOPXN010000055        13431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4698_length_13431_cov_5.082835, whole genome shotgun sequence.
ACCESSION   DAOPXN010000055 DAOPXN010000000
VERSION     DAOPXN010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13431
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4698_length_13431_cov_5.082835"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PKM49_04570"
     CDS             <1..346
                     /locus_tag="PKM49_04570"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HNY82121.1"
                     /translation="NVILDFTLAPIQGAYGVALATSFAGMTPVAFLLVHLIRIKGLTM
                     TRRQLSELLKTVSATAVMSAVLLMIAKTYSYSKPLVVLELVCGVLVYLLALLLLRKQE
                     MKRLILRWSRRG"
     gene            404..477
                     /locus_tag="PKM49_04575"
     tRNA            404..477
                     /locus_tag="PKM49_04575"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:436..438,aa:Gln,seq:ctg)
     gene            complement(481..1182)
                     /locus_tag="PKM49_04580"
     CDS             complement(481..1182)
                     /locus_tag="PKM49_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY82122.1"
                     /translation="MNRSFAEFERSQNYYEAITGASEGIPKEHRITHATTLLLWYCGI
                     PPKDAFFAEIGCGVGFSMCWMAKTHGIRGIGIEKQTELKDSFDRLAQRAGVSGQVSFL
                     PLSAEEVPKAYSAGSCDFVFSNPPHFEHQRGKSYRDSIRQTFRSLEGPAYESFCFAIA
                     HLLKPRRRFYLVLAPQYLPEWIEHLSELRLRVKNLCFVHGKPGKHAELVLVKGIKEGN
                     PGFLVVDPPVFLKEG"
     gene            complement(1172..3391)
                     /locus_tag="PKM49_04585"
     CDS             complement(1172..3391)
                     /locus_tag="PKM49_04585"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HNY82123.1"
                     /translation="MGRKGRDFLLFLPILLSLLALFSSCAQVSGRINPPETKIETKTQ
                     KFGPSGVTIAFSGKDERTPPEKLTYAYWVYRLVEPTEKNAKSDPFTFERKPVLEASST
                     VAQAALDSQRLKEGQFLFAVVAVNEFGLADESPAEHVFRIDLTPPAPPQVEAILKAGK
                     VKVACDYDDTPEDLAGYAFTALSNNDSRTTEQPIGIWTFPAKLGEVYEIEVYAYDDVG
                     NRSKTATLECDASEDHPPVLLTALPALLGVKTVEIELDYYDDWDSQTQIAIEATISSV
                     ALKIKDGRLQINYDQLSEGTNTILLELKDTNKHVHRVQKEAFVDLTPPQIPDELSIQN
                     LSNGYMVSWKKAVEADSYALYGSNDGNDWEQIGTTNDQKLYSPQRFLHFAVSAFDKAG
                     NESHISYPVRTYDEMYSPVISSTIDSIGGNTLLTTLFSPYLIKSHLVIPDNVSLAFER
                     GVEITFVDEGELEIEGELISIPSRTDRYTRFLIQREPNGQPFIKVGKGNVWLEKIIFE
                     NPGKNGWLFAMSRNSNILINDAQIKGFETVVCCNSSDSFQITESEVEAENFVVGDSVK
                     NMKITCSAIRAKNGISLNNVQNLFLSETILECGESLMTTSGLSNIKIENSTLLSSRNG
                     IQAQKLTVIELFDSSISASNTALAINGASSLNIRKSKISDSEVGVSAMQSSYVSISES
                     VISGCDRGVQVVNSDLSINDSTFEQNATAVALQGRNAFEQKKVVFTGNDLDISRDEP"
     gene            complement(3563..6292)
                     /locus_tag="PKM49_04590"
     CDS             complement(3563..6292)
                     /locus_tag="PKM49_04590"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HNY82124.1"
                     /translation="MAPEKHLALFYQDREHQRRMMMPFFSECLKQDLKVLYITNDETT
                     RWIDGLFSKLTVSLDKHPKDQQIKAWTAESVYLREGTLNFDSIIRILQREEKRAESEG
                     FEGLSFCLEVTHILLKLYSLSSFRTFMNALNVFLAGNHRILFLYQSSIIEAVSLMQIL
                     TAHPYIAVGDEFIKNPYYTAPEKNPAQVFFTYLMQNHEYLETIAVNREKIREITLARD
                     RFEGLWRSATGALDETKGALTSLEQALKEKTASEENFREEAERLLRLSSEQARGLSVM
                     EERHKELVEREQVQNQETQRLQALLSQAEIRDSERTDRVTWLESQNHHLKDELERLEK
                     EKSEAESKFSEFFQGKALEIKSLEESQMKERIEWNEVKRDLEDRVGDLTQQSLREKSR
                     FEEEISAYIRAIDRLETELANQKSLTIESEIERHRLEASLAEQEETLDLRRQEVRSAQ
                     ETLLKEKEEWTAKIKVLETQLTLGIEEGRRTEKALKEALSEKAAQNDRIEALTLESSM
                     AKTERTDLFRELEQVKALHKAETESRQESERKTGALLAEREQYRSEKERLRREIETVS
                     DRIEALSAELNALEVEKQAWKEERSGYLEKIAQLTEQIERTAQMKDELELFCEDARIR
                     SENSSRELESARQTILELSAQKEGLLQDLENGKAQITSLSAQVEEAKEMAERKRKCLG
                     DLKLRLEQQETSELRLRKELAESNKSLAESRALNEDLNAKELECRDRIEHLKIRLDQE
                     ESLFQEVQKEIFSYKAQISILEERLKDEEIRHREKDRFAADLTGLVELLKTENEEVRE
                     QYRQIRDLKNRLETLWMAKGLEPGIARKNERLASAYSLGIVSKCAAPDDGEVGDHECS
                     QEEREERDVQVKLLQETVESLQRERSELLSLLEVADDELKKAQRPEEDFLSRD"
     gene            complement(6405..7361)
                     /locus_tag="PKM49_04595"
     CDS             complement(6405..7361)
                     /locus_tag="PKM49_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNY82125.1"
                     /translation="MKKPIAGLFFTLLFCLAAGILYAGGPEGILSEAIRGFTGDHVVG
                     EPIVAGEVVIIPIFSADFSFGEISGGPDGEVWGSGASGKLNFLPYAIAIVDQKGVRVL
                     PVSNKKTFFEQVVEALPKLMPIVQQVIGYFSLNSGSAALEMPKTGSEQIIDVETLQPT
                     DRVAELYHRVQLGLPPEDYQEILAEARELLPEDPGNAKLHSVNAYATMMMIQKAGPLQ
                     QIRMALEAQKEIDTALSLDPNDILGLISQGWMNLHNPMGRIDLAEAAFEKVLSIDPEH
                     LEALMGLTMVYEKMGRSEKVKEWAEKGLALDPENEYFLNLIH"
     gene            complement(7358..7852)
                     /locus_tag="PKM49_04600"
     CDS             complement(7358..7852)
                     /locus_tag="PKM49_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82126.1"
                     /translation="MKKVFSIAVAVFFILVGVLGLLSSLTTFSIDWRFLGAAIPLFIG
                     LFFQVQYFTEKKEPGLLVPGGTLTVIGILVLLDFFFTDSVSEYIWPFYILAPAVGLFQ
                     FYWFGKRDRALLIPVTVLSVVCVVFLLVTLYQIPPFSYILPILLLVIGTFMLYTYFRK
                     GRKP"
     gene            8517..8747
                     /locus_tag="PKM49_04605"
     CDS             8517..8747
                     /locus_tag="PKM49_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82127.1"
                     /translation="MAILEDNFYLGVRAVASLKNNAERLVQRNKVTGEEAWAFSTLTR
                     PEHVTKEEFTMPFEMRVKIEETKGLGPNPLWK"
     gene            8738..9325
                     /locus_tag="PKM49_04610"
     CDS             8738..9325
                     /locus_tag="PKM49_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82128.1"
                     /translation="MEMIIVVSVVLLTVTVVLVMVFAYLRHMKTQQASPHLDISDLEE
                     RVLELVNRFQHISATRINTFEQKIKEMSVLIQHANESYMKLTSVISEAYKAESELLER
                     SRKKEMETLFGKLRSEKVESENVVEYIGSTVSEKREPFSPVPPDAVDLRTSSIEHQIL
                     NLSSEGQSAEDIAESLQIGRGEVQLVLELFRRKKK"
     gene            9341..9853
                     /locus_tag="PKM49_04615"
     CDS             9341..9853
                     /locus_tag="PKM49_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82129.1"
                     /translation="MRSVLREKSQDFFEILRYDRRDWMVFWDRYISENEEFMAGYCPA
                     LGLDREAVRAHLYAFERRFLDRLKMENETIRRIKGKTVNALSAIQGQLKLNQADFTVY
                     MAGGLGVREFIAYRESRGFVVLMDIIALKKLDHLARMPELTVACVQQIRKVLDSPEGG
                     SVWVSKELAS"
     gene            9832..10791
                     /gene="pfkA"
                     /locus_tag="PKM49_04620"
     CDS             9832..10791
                     /gene="pfkA"
                     /locus_tag="PKM49_04620"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNY82130.1"
                     /translation="MKRIGILTSGGDSPGMNAALRAATRYGIKMDLEVYGISRGFAGI
                     LDEEIAPLTFKDVAGTMERGGTILRTARCPEFKVAQVRDEAAQKLKKHGIEALIVIGG
                     DGSLSAGALLNDDHGVPCVGIPGSIDNDIYGTDMSIGVDTCLNTVMDAIQKLKDTASS
                     HERAFIVEVMGRECGYIAMMSGIITGAEAAIIPEVPVDYDKLAERLLEERKRGKINTI
                     IVVAEGAASAYTVARHLEHRIGYETRITILGHVQRGGSPSAFDRVIATRMGIHAVKSI
                     MEGQYGVMVGLQAQGIARVPFSDVLTKKKKLNMELYDISFLLS"
     gene            10811..12238
                     /gene="pyk"
                     /locus_tag="PKM49_04625"
     CDS             10811..12238
                     /gene="pyk"
                     /locus_tag="PKM49_04625"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNY82131.1"
                     /translation="MRKTKIVSTLGPASNTETMIERLIDAGVNVFRLNTSHGKIEEHT
                     ETVRLIKRIRERRHAPIGILIDLEGPKIRIGPFQSGSVLLTKGQSFILTAQDILGDEN
                     RVSVSHKRLPKEVRSGDSIMIADGLIELKVRSADGTEIVTIVENGGPLSGRKGVNAPG
                     VDLGLEAITEKDKTYIRMAVEEGADYLAQSFVRKPEDIDVCRSEIRRLDSSIPIIAKI
                     ETKQALGYLNSILYKSDGVMVARGDLGVEIPAQEVPLIQKKLIAMANAQSKTVITATQ
                     MLESMIENPRPTRAEASDIANAILDGTDAIMLSAETTVGRYPVEAVRMMADIANVIEK
                     NWESFQTRPFENPLIQEIFLDNPEEAIAHATCDIARQLAIEVIVTSTVSGKTARMISK
                     YKPPSTLLGITPELATYYRLALVWGVWPVLVERTRSTDEMMAIAERKAMDCGLVKKGE
                     RLLLTAGIPWGHSGSTNMLKIHTCS"
     gene            12187..13371
                     /locus_tag="PKM49_04630"
     CDS             12187..13371
                     /locus_tag="PKM49_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82132.1"
                     /translation="MGTFRVHKHVKNSHLFLTLLLLLLYSALFGQAIDIKVSTTGAMT
                     ESESAFIKEFRNEFASMYERNASGIFYSAKAMAVSLSFGKENSTFFLRAAIEGQLFYT
                     EKGFRSLDRLALSQSVTQCVENLLVWFFLRRAFQTEKRIGLSDLLYEYPHFSADGRFF
                     TYITEKRTGNANPLVVSLTDGTVFLLELPMKTEFYPRVYGEFLYYLQSLEGAREILRV
                     PLSRLSDRREGLYEGDVTGYSVRGNELIWSEKNRLLRKPLDGKGPYSEEPLPPNASRI
                     QSFDIADHYWAISLAREGAQYDLYLYDPVRKTGLFLTHTPYQEVDVRFSKDGTMLVFS
                     SNPDGNFNLFYFDLVNNRLGRVTQNAYDEFYPAFTPDGSGLVFCRYETKSEPYLVALP
                     LR"
BASE COUNT         3207 a         3489 c         3430 g         3305 t
ORIGIN      
        1 gaacgttatt ctagacttca ctttagcgcc gatccaaggc gcttacgggg tcgccctcgc
       61 aacctctttc gcggggatga cacctgtcgc tttcctgctc gtccatctca tcaggataaa
      121 agggctcaca atgacgcgaa gacagctctc cgaactcctg aaaacggtca gcgcgaccgc
      181 ggtgatgagc gccgtcctgc tgatgatcgc taagacctac agctactcca aacccctcgt
      241 ggttttagag ctcgtgtgcg gcgttttggt gtaccttttg gccctactcc ttttgagaaa
      301 acaggagatg aaaaggctga ttctcagatg gagtcggagg ggctgaccgt agcaggcgcc
      361 cgtttgacat cgatagggct ttcgtgtata attgcgggtg ttttgggaga tcgtctaacg
      421 gtaggactac ggactctgga tccgtaagtg gaggttcgaa tcctcctctc ccagccagac
      481 ttaaccctct ttcaaaaaaa ccggaggatc gaccaccaag aaaccggggt ttccttcctt
      541 tattcctttc accaaaacga gttccgcgtg ctttcctggt ttaccgtgaa cgaaacacag
      601 gttcttcact ctcaatcgaa gctcggagag gtgctctatc cattctggta ggtactgagg
      661 ggcgaggacc aaataaaagc gccggcgcgg tttgaggagg tgcgcgatcg cgaaacagaa
      721 gctttcgtag gctgggccct ccagggagcg aaaggtttgg cgaatcgaat ccctatagga
      781 tttcccgcgt tgatgctcga aatgtggagg gttggaaaac acgaagtcgc aagaaccggc
      841 ggagtaagct ttgggaacct cttcggcgga gagtggaaga aaagacacct gtccggacac
      901 cccagctctt tgcgcaagac ggtcgaaaga atcctttagt tccgtttgct tttcgatacc
      961 gattccccgt atcccgtggg tcttcgccat ccagcacata gaaaaaccga cgccgcaccc
     1021 gatttcggcg aagaaggcgt ctttaggggg aatcccgcag taccatagca acagagtcgt
     1081 ggcgtgggtt atccgatgct cttttggaat cccttcactc gctcccgtta tcgcctcgta
     1141 gtagttttgc gaccgctcga attcggcaaa actacggttc atctcggctg atatcgaggt
     1201 cgttgcccgt aaagaccact ttcttttgct cgaacgcgtt acggccttgt aaagccacag
     1261 ccgtggcgtt ttgctcgaag gtggagtcgt tgatggacag gtcgctgttg accacttgca
     1321 cgccccggtc gcatccgctt atcacgcttt cgcttataga gacgtaactg ctctgcatcg
     1381 cggacacacc cacttcggaa tcgctgatct tcgatttacg tatattcaag gaagaggcgc
     1441 catttatcgc tagggccgta ttagatgcgg agatcgagga atcgaacagt tcaatgactg
     1501 tgagcttctg agcctgtatg ccgttacgcg aggaaagtag ggtcgagttc tctatcttga
     1561 tgttgctgag tccggatgta gtcatcaatg actcgccgca ttccaaaatg gtctcggata
     1621 agaaaaggtt ctgcacattg ttcagactaa tgccgttttt ggctcgaatc gcgctgcacg
     1681 tgatcttcat attcttcacg gagtctccaa cgacgaagtt ttcggcctcc acctcggact
     1741 ccgtgatctg aaagctatcg gagctattgc aacacacgac ggtttcaaaa ccctttattt
     1801 gggcgtcgtt gattaagatg ttgctgttcc ggctcatcgc gaacaaccat ccgtttttcc
     1861 ccggattttc aaaaatgatc ttttccaacc atacgttacc ctttccgact ttaatgaagg
     1921 gttggccgtt cggttccctc tgaatgagaa aacgagtgta ccggtccgtc cgagagggga
     1981 tggagatcag ttcaccctct atttccaatt caccctcgtc cacgaaggtg atctcgactc
     2041 cccgctcgaa ggccaaagaa acgttatctg gaatgaccag atggctttta atgaggtaag
     2101 gggagaagag cgtcgtcagg agcgtattgc ctccgatgct gtctatcgtg gagctgatca
     2161 ccggagagta catctcatcg taagttcgaa cggggtagga gatatggctt tcgttgccgg
     2221 ctttgtcgaa agcagaaacg gcaaaatgca gaaaccgttg gggcgaataa agtttctgat
     2281 cgttggtcgt gccgatctgt tcccaatcgt taccgtcatt ggatccgtac agcgcataag
     2341 aatcggcctc caccgccttt ttccaactga ccatatagcc gttcgacagg ttttgaatag
     2401 acagctcatc gggtatttgc ggaggcgtca ggtctacgaa ggcttccttt tgcactctgt
     2461 gtacgtgttt gtttgtatcc ttcagctcca ataagatggt gttcgtgcct tcggaaagct
     2521 gatcgtagtt gatttgaagg cgaccgtctt tgattttgag ggctacgctg gagatcgtcg
     2581 cctctatggc tatttgggtc tgcgaatccc aatcatcata gtagtccagt tcgatctcaa
     2641 cggtttttac gcctagcaga gcggggagcg ccgtaagcaa gaccggtgga tggtcttccg
     2701 aggcgtcgca ttcgagggtt gccgttttgg agcgatttcc cacatcatcg tacgcgtaca
     2761 cttcgatctc gtaaacctct cctagcttcg caggaaaagt ccaaatcccg atgggttgct
     2821 ccgtagttcg cgaatcgttg ttggagagcg cggtgaaggc ataaccggca agatcctccg
     2881 gcgtgtcgtc gtagtcacag gccaccttga ctttgcccgc tttcagaatc gcttcgacct
     2941 gcgggggagc aggcggggtc aggtcgattc ggaaaacgtg ctctgccggg ctttcgtcag
     3001 ccaacccgaa ctcgttcacc gccacgacag cgaacaagaa ctgcccttct ttaaggcgct
     3061 gactgtccaa ggccgcttgc gcaacggtag aggaggcttc gagaacaggc tttctttcga
     3121 aggtgaaggg gtcgctcttc gcattttttt ccgtaggttc aacgagtcgg tagacccaat
     3181 aggcgtacgt gagtttttct ggcggggtcc gctcatcctt gccggaaaag gcaatggtga
     3241 cacccgaagg gccgaatttt tgagttttcg tctcaatttt cgtctcaggt gggtttatcc
     3301 gaccgctcac ctgcgcacaa ctcgagaata gcgcaagaag cgatagtaag atcggtaaaa
     3361 atagaagaaa atctcgaccc tttctcccca tataatgtcc ccctctttaa gtttctccga
     3421 tcaaaaaaga ataaataaag aatcgtggcc atagggatta tagaataaga gcgcgtgtag
     3481 acacaagtat tctgtcacaa cgtgttcttt tccgctcaca tcggccactc ccttttgatc
     3541 gttctcgctt ctccattcgg ttttagtctc tgcttaaaaa atcctcttcc ggtctctgag
     3601 ccttcttgag ctcgtcgtcg gctacctcta ataacgagag caattcggaa cgttctctct
     3661 gtaaactctc gacagtctct tgaaggagtt tgacttgaac gtccctctcc tcccgctcct
     3721 cttgactaca ctcgtggtct ccgacctccc cgtcgtcagg ggcggcacat ttagatacga
     3781 tcccgaggct ataggcggac gctagtcttt cattctttcg agcaatccca ggctctagcc
     3841 ctttcgccat ccaaagagtt tccaagcggt ttttcaaatc cctgatctgc cgatactgtt
     3901 cgcgtacctc ttcgttttcg gttttgagca actcgactaa ccctgtcaaa tcggccgcga
     3961 atcggtcttt ctctcgatgt cgtatttcct catctttgag acgctcctcc aagatgctga
     4021 tttgcgcttt ataggagaaa atttccttct gaacctcttg gaaaaggctt tcttcctgat
     4081 cgagccgtat cttcaagtgc tcgatgcggt ctcggcattc gagttctttc gcgttcaagt
     4141 cttcgttaag ggctcgagat tcggccaatg atttgttcga ttccgccaac tcttttctta
     4201 gacgcagttc ggaggtttct tgttgttcca accttagctt caaatccccc aaacatttgc
     4261 gtttcctctc agccatctct tttgcttctt cgacttgagc agacagcgag gttatctggg
     4321 cctttccgtt ctccaagtct tggagaagcc cttctttctg cgcgctcaat tctaggatcg
     4381 tctgccttgc gctctccaat tcccgcgaag aattttcgct ccggattctt gcgtcctcgc
     4441 agaacagctc gagctcgtct ttcatttggg ccgttctttc tatttgctcg gtcaactggg
     4501 cgatcttctc caagtagccg ctccgctctt ctttccaagc ctgtttctcg acttccaggg
     4561 cgttcaattc cgcggaaagg gcttcgatac gatcggagac ggtttcgatc tctcgcctta
     4621 acctttcttt ttcagaacga tactgttctc tctccgcaag caaagccccg gttttccgct
     4681 cgctttcctg cctggattcc gtttctgcct tatgaagcgc cttgacctgc tcaagctctc
     4741 taaacaagtc cgtgcgctcg gttttcgcca tagaagactc caacgtaagc gcttcgatac
     4801 ggtcattctg tgcggccttc tcactcagtg cctctttaag ggctttttca gtgcgtcggc
     4861 cttcttcgat ccccaacgtt aactgggttt cgagcacctt gatcttcgcg gtccactcct
     4921 ctttttcctt caagagtgtt tcttgggccg agcgcacctc ctgacgacgc agatcgaggg
     4981 tttcctcctg ctccgccaaa gaagcttcca agcggtgtcg ctctatctca gattcgatcg
     5041 tcagactttt ctgattggca agttcggttt ccaacctgtc tatggctcga atgtaagcgg
     5101 agatctcctc ttcaaaccga ctcttttctc tcagggactg ttgcgtcaaa tccccgaccc
     5161 tatcctctaa gtctctcttg acctcgttcc actctatccg ctctttcatt tgactctctt
     5221 cgagcgattt gatctccagc gcctttcctt ggaaaaattc agaaaacttc gactcagctt
     5281 cggacttttc tttctcgaga cgttccaatt catccttaag atgatggttt tggctctcca
     5341 accaggtcac ccggtcggtc cgttctgagt cgcggatttc ggcctgagaa aggagcgcct
     5401 gtaagcgttg ggtctcttgg ttttggactt gctccctctc tacgagctcc ttgtgccgct
     5461 cttccataac cgataggcca cgggcctgct cggacgacaa tcgcagcaaa cgttccgcct
     5521 cctctcgaaa gttctcctcg gaagcggtct tttccttcaa cgcttgctcc aaagaggtca
     5581 gagccccctt ggtttcgtcc aacgcgccgg tcgcggatcg ccacaggcct tcaaagcgat
     5641 cgcgcgcgag ggtgatctct cggatctttt ctcgattcac ggcgatcgtt tccaagtatt
     5701 cgtgattttg catcagatag gtgaagaaaa cttgcgcagg gttcttttcc ggagcggtat
     5761 agtatgggtt cttgatgaac tcgtcgccca cggcgatgta gggatgggcg gttaaaatct
     5821 gcattaacga taccgcctcg atgatcgagg attgatatag aaacagaatc cggtgattgc
     5881 cggcgagaaa gacgttcaag gcgttcataa aagttctgaa agaagaaaga gaatagagct
     5941 tcaagaggat gtgggtgact tccaaacaaa aggataaccc ttcaaaaccc tcagactccg
     6001 cacgtttttc ctcccgctgc agtatcctga tgatcgaatc gaaattcagg gttccttctc
     6061 tcagataaac ggattcggca gtccacgcct tgatctgttg gtctttcggg tgtttgtcca
     6121 aagaaacggt gagcttcgaa aataaaccgt cgatccaccg cgtggtttcg tcattggtga
     6181 tgtagagcac cttgagatct tgcttcaggc actcggaaaa aaacggcatc atcatacgtc
     6241 tttggtgctc acggtcttga tagaaaagcg cgagatgttt ttctggcgcc atcgcaatcc
     6301 cccccataga tcttactcac acaggacaaa aatagacgaa agcgctgtta agcttcgctt
     6361 tcgtccggta tctcccatcc attcctcacc tgaatggaac aggctcaatg gatgaggttt
     6421 aagaaatact cgttttccgg gtccaaagcc aacccttttt cggcccattc cttgaccttt
     6481 tcggaccgac ccatcttctc atagaccatc gtgaggccca tcagcgcctc gaggtgttcg
     6541 ggatcgatag acaacacttt ttcgaaggca gcctcggcga ggtcgatacg gcccatcgga
     6601 ttgtgtagat tcatccaccc ttggctgatc agacctaaaa tatcgttggg atccagagac
     6661 agcgccgtgt cgatctcttt ttgagcttcc agcgccatgc ggatctgttg caacgggcct
     6721 gctttttgga tcatcatcat cgtcgcatag gcgttcaccg agtgaagctt ggcgtttccc
     6781 gggtcttcag gcagcaactc gcgcgcttcc gctaagatct cctgatagtc ttcgggtggt
     6841 aagcccagtt gaacacggtg gtagagttcg gccactcgat cggtgggttg taaggtttcc
     6901 acgtctatta tctgttcgga gcccgttttt ggcatctcga gggcggcgct cccggaattt
     6961 aaagaaaagt acccgatcac ctgttgaacg atcggcatta gttttggaag cgcttctacg
     7021 acctgttcga agaacgtttt tttattcgac accggcaaga cgcgtacgcc cttttgatcc
     7081 acgatggcga tcgcgtaagg aaggaaattc agctttccgc ttgcgccgct tccccacact
     7141 tctccgtcag ggcctccgga gatttctccg aaagaaaagt ccgcggaaaa gatcgggatg
     7201 atgacgacct ctcccgccac tataggttcc ccgaccacat gatcaccggt gaacccccgt
     7261 atcgcttccg ataagattcc ctctggaccc cctgcgtaaa gaatgcctgc ggctaaacag
     7321 aacaataatg tgaagaatag ccctgcgata ggttttttca cggttttctc ccctttctga
     7381 agtaggtgta aagcataaag gttccgataa cgagcagcaa gataggcaag atataagaaa
     7441 acggtggaat ttgatataga gtgaccagga gaaagacgac gcacacgacg gaaagcacgg
     7501 taaccggaat cagcaaagcc cgatctcgtt tgccaaacca atagaactgg aaaagaccca
     7561 cagccggggc gaggatatag aaaggccaga tatactcgct caccgagtcg gtgaagaaaa
     7621 aatccagtag gactaatatg ccgatcaccg tcaaagtacc ccccggcacc agtaatccgg
     7681 gttctttctt ctcggtaaag tattgcactt ggaaaaagag cccgatgaat aatgggatcg
     7741 ccgctcccaa aaaccgccaa tcgatcgaaa aagtcgtaag ggaagacaac aacccgagca
     7801 ctcctaccaa gataaaaaag acggccaccg cgatagagaa cacttttttc atctacctca
     7861 caccctcgcc aacagacggt acctttatag agttttatta tagcacaaac agtaccgttt
     7921 caatcctcgt tgtgtgccaa gttatgaggt gtaatgaaga aacaaggtca gaatcgactt
     7981 cgcgattttt agtccatata gtcatcgcgg cgtgagcgtc ccatctcttc gtggggccga
     8041 cttcgagtgg gcctgcctct ctgggaacac cgatattata cccacactac agtttagaaa
     8101 aagcaaaaac acgcggatca gggatcgata gtattacagt tttaacctcg gaccacagta
     8161 ttcttgatat atataactcg taaaagcgaa ccaaaccata gcggagtacg ccatttttat
     8221 gcccacacgc gtttttgtta taagtcgtct tcgtgatata atctcatatt cttaaactct
     8281 cttcggagag ggtggcgcgc aggaaaggac cggtggaaga atactaacag acggttatat
     8341 aaaaatccga tcgcaaccgg tggtccttca acatcttatt tgttcagaca atgggcgctg
     8401 cggcactgat tggcttgtac aaccgtaaaa ggaatcgtag ggcggcacaa acgactttcg
     8461 ctatctgttg gctcgacgtt cgtttacggg gtcttcttct gtgaaagggg ggagctatgg
     8521 ccatattgga agataacttc tatctcggag tgcgggcggt ggcttcgcta aaaaataacg
     8581 ccgaaaggct cgtccaacga aacaaggtaa ccggagaaga agcctgggcg ttctccacgc
     8641 tcacccgacc ggaacacgtg acaaaagaag aattcacaat gcctttcgaa atgcgcgtga
     8701 aaatagaaga aacgaaagga ctcggaccga accccctatg gaaatgatca tcgtcgtcag
     8761 cgtcgtgctg ctcaccgtaa cggtcgtgtt ggtgatggtg ttcgcttact tgcgccatat
     8821 gaaaacgcaa caggcctctc cgcatctgga catctccgac ttggaagagc gagtattgga
     8881 actggtcaat cgttttcagc acatatcggc tacgcgcatc aacacgttcg aacaaaagat
     8941 taaggagatg tccgtattga tccagcacgc gaacgagtct tatatgaaat tgacctccgt
     9001 tatctcggaa gcctataaag cggaatcgga gcttttggaa cgttcacgaa aaaaagagat
     9061 ggaaaccctc ttcggaaagc tccgttcgga gaaagtagaa agtgaaaacg tggtagaata
     9121 catcggttcg acggtctcag aaaaacgcga accctttagt cccgtaccgc cggacgcggt
     9181 tgacctccga accagctcga tcgagcatca gatactcaac ctttcctcgg aagggcaaag
     9241 cgcggaggac atcgccgaat ctcttcagat aggaagagga gaggtacagt tggttctaga
     9301 gctattccgt agaaagaaaa aatgaacggg ggcagtgggc gtgaggagtg tactgaggga
     9361 gaaatcgcaa gattttttcg aaatcttgcg gtatgatcga agggattgga tggtattttg
     9421 ggaccggtat atttctgaga atgaagagtt tatggcaggc tattgtcccg ctttaggctt
     9481 ggatcgggag gcggtcaggg cgcatctata cgccttcgag cggcgatttc tcgaccgttt
     9541 gaagatggag aacgagacga tcaggagaat caaggggaaa acggtaaatg ccttatccgc
     9601 catccaaggg cagctgaaac tgaaccaagc ggacttcacg gtctatatgg ccggagggct
     9661 gggggtgcgc gagttcatcg cgtatcggga atcacgcggt ttcgtcgttc ttatggatat
     9721 catcgcgttg aaaaaactag accatttggc gcgtatgccg gaattgaccg tcgcatgcgt
     9781 tcaacagata cggaaggttt tagacagtcc ggagggaggc tcagtatggg tatcaaaaga
     9841 attggcatct taacgagcgg gggagactct cccggtatga acgccgcttt gcgtgctgct
     9901 actcgctatg ggataaaaat ggatttggaa gtgtatggca tatcccgggg cttcgcgggt
     9961 attctggatg aagagatcgc tcccctcaca ttcaaagacg ttgccggaac gatggagcgt
    10021 ggagggacca tcctgcgcac agcgagatgc ccggaattca aagtcgccca agtgcgggat
    10081 gaagccgctc agaaactcaa aaagcacgga atcgaagcct tgattgttat cggtggagat
    10141 ggcagcttgt ccgcgggcgc tttattgaat gacgatcacg gggtaccgtg cgtaggcatt
    10201 cccggcagca tcgacaacga catctacggc acggatatga gcatcggcgt ggacacttgc
    10261 cttaataccg tgatggatgc catacagaag cttaaggaca ccgcctcatc acacgagcgg
    10321 gccttcatcg ttgaagtgat gggtcgggaa tgcgggtaca tcgcgatgat gtccggaatc
    10381 atcaccggcg cagaggcagc gatcattccg gaggtcccgg tcgactatga caagcttgct
    10441 gagcgcctgt tggaagaacg caagcgagga aaaatcaaca cgattatcgt tgtcgccgaa
    10501 ggcgccgcca gcgcatacac ggtcgcgcga cacctggagc accgcatcgg atatgagaca
    10561 cggataacga ttttagggca cgtacagaga gggggaagcc catcagcctt cgatcgagtc
    10621 atcgccacac ggatgggcat acacgccgta aaatccataa tggaaggcca atacggcgtg
    10681 atggtcggcc tgcaagccca aggaatcgcg cgggttcctt tttccgatgt ccttacgaaa
    10741 aaaaagaagc tgaatatgga actctacgat atctccttcc tactttcctg aagataggag
    10801 gagcagatcg atgcggaaaa ccaaaatcgt tagcacgctc ggacccgcct cgaatacgga
    10861 gacgatgatc gagcgcttga tagacgcggg agtgaacgtg tttcgcctga acacctctca
    10921 cgggaagata gaagagcaca ccgaaaccgt acgcctgatc aagcggatac gcgagcgccg
    10981 gcacgccccg atagggatac tgatcgattt ggaggggccg aagatacgga tcgggccatt
    11041 tcaatccggt tcggttttac tgaccaaagg gcaatccttc atcttaaccg ctcaagatat
    11101 attgggagat gagaatcggg tttccgtgtc ccataagcgc ctgccgaaag aggtgaggtc
    11161 cggtgattcg ataatgatcg ctgacgggct gatcgagctg aaggtacgaa gcgccgatgg
    11221 caccgagatc gtcacgatcg tagaaaacgg agggccgctc tccggaagga aaggggtcaa
    11281 cgcgcccgga gtggacctcg gtttggaggc aatcacggaa aaggacaaga cgtacatacg
    11341 gatggccgta gaggaagggg cagactacct cgcccagtcc ttcgttagaa aacccgaaga
    11401 catcgacgta tgccgaagcg aaattcggcg gttggacagt tcgatcccga tcatcgcgaa
    11461 aatagagacg aagcaagctt tggggtacct caacagcatc ctttacaaat cggacggagt
    11521 tatggtcgcg cgcggtgatt taggggtaga aatacctgct caggaagtcc cgctgatcca
    11581 gaagaaactg atcgctatgg cgaacgcgca gagcaaaacg gtgatcacgg ccactcagat
    11641 gctggagtcg atgatcgaaa acccccgccc tacgcgtgcg gaggcttccg acatcgccaa
    11701 cgccatcctc gacggtaccg acgctatcat gctctcagcc gaaaccaccg taggcaggta
    11761 tccggtggag gcggttcgta tgatggccga tatcgccaac gtgatcgaaa agaactggga
    11821 gagcttccag acgcggccct tcgagaatcc cctgatacag gagatctttt tggataatcc
    11881 ggaagaagcg atcgcccacg cgacttgcga tatagcgcgc cagttggcga tcgaggtcat
    11941 cgttacttcc accgtcagtg ggaaaacggc ccgtatgatc tcaaagtaca aacccccgtc
    12001 cacgctccta ggaatcaccc ccgaactcgc cacttactat cgtttggcgt tggtatgggg
    12061 agtgtggcca gtcctcgtgg aaaggacacg aagcacggac gagatgatgg ccatcgccga
    12121 acgaaaggcg atggactgcg gtttagtcaa gaagggagag cgcttgttat tgacggcggg
    12181 gataccatgg ggacattccg ggtccacaaa catgttaaaa attcacacct gttcctgacc
    12241 ctactgctcc tactcctcta ttccgctctg ttcgggcagg ctatcgacat aaaggtttct
    12301 acgaccggcg cgatgacaga atctgaatcc gccttcatca aagaattccg gaacgagttc
    12361 gcctctatgt acgaaagaaa tgcctccggc atcttctatt cggccaaagc gatggcggtt
    12421 tctctctcat tcggtaaaga aaactcgacc ttcttcctgc gggccgcaat cgaaggccag
    12481 ctcttctaca cggaaaaagg gtttcggtct ttagaccggc tcgccctttc gcaaagtgtc
    12541 acccaatgcg tggagaacct gttggtatgg ttctttttgc gccgcgcctt tcaaacggaa
    12601 aaacgcatcg ggctatccga tctcttgtac gaataccctc atttttccgc cgacggccgc
    12661 ttcttcacct atatcaccga aaaacgaacg ggcaacgcca accctttggt cgtgtcattg
    12721 acagacggga ccgtcttttt actggagctg cctatgaaaa ccgagttcta cccgcgggtg
    12781 tacggcgaat tcttatacta cctccaaagc ttggagggag ctcgggagat actgcgggtg
    12841 ccgttgagtc gattaagcga caggagagag ggactctacg agggagacgt caccggatat
    12901 agtgttcgcg ggaacgaact gatatggagc gaaaaaaacc gtctattgag gaaaccgcta
    12961 gacgggaagg gcccttatag cgaagagcct ttacctccaa acgcttcgcg tatccaatcc
    13021 ttcgacatcg ccgaccacta ctgggcgata tctttagcgc gggaaggggc gcagtacgat
    13081 ctctatttgt acgatccggt ccgtaaaacg gggttatttt taacccacac cccctaccaa
    13141 gaagtcgatg tccgattttc caaagacggg acgatgttgg tcttttcgtc taacccagac
    13201 ggaaacttca atctcttcta tttcgacctc gtcaacaatc gcttgggccg agtgactcaa
    13261 aacgcttatg atgaatttta tcccgctttc acaccggacg ggagcggcct ggttttttgt
    13321 cgctatgaaa ccaagtccga gccctatctg gtggcccttc cgcttcgatg actttctccc
    13381 gactttcgac cggttttgga gaaaaaggga aaaaataaga aaaaacaacg c
//
LOCUS       DAOPXN010000056        13404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4710_length_13404_cov_4.835868, whole genome shotgun sequence.
ACCESSION   DAOPXN010000056 DAOPXN010000000
VERSION     DAOPXN010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4710_length_13404_cov_4.835868"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            625..2022
                     /locus_tag="PKM49_04635"
     CDS             625..2022
                     /locus_tag="PKM49_04635"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY82133.1"
                     /translation="MAKKKLNRFWALILILNPIASLALGLWVFSLPGTLGLVRWDSFY
                     RLLVGLVLSLGLWAPVLWVVGRLFLRKRKWLAKIWGVGVAALSAAILAFTLGAWGYLI
                     AQPMLKSGDKPPVLLVSSHSGAAMPDLTLAVWTKTPQQLTASLGSETEPTRSILSDSG
                     RVHRLLFPKLQPERVYTYQLEGGESVSFRALPDIALEGSKPMKVVFSSDVHYGSSKSS
                     AEKTTRMMDQWLSRKDEIDLFCLLGDITELGFMDSDWQDALDSLSPVFSQIPMIPVIG
                     NHDLLFQGYRHYLDYLHPEGRTSFEDLYRRVDIGPVHFLLLNAVWGTEDVSREQLAWL
                     EAQLASIPKDHWKVVLSHAYYYASGSTFAGEPWYDNREMIRIFGPIFEKRGVDLVVSG
                     HNHLLEVLEHAGVTYAVVGVMGAPLDTITYRSPASVWCRDVHGWMEVTWTAREATLQF
                     FQWDGVPLFSQTLTK"
     gene            2546..3772
                     /locus_tag="PKM49_04640"
     CDS             2546..3772
                     /locus_tag="PKM49_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNY82134.1"
                     /translation="MDALTDLGVKKMSWAWQYMPILNELHQKYKDEKPFAGCKMVVCC
                     HLEAKTANLARLFRDTGAQVWTTGSNPLSTQDEIVAALANHEKMVVHAKHTEDNEAFR
                     KNLYKALELKPNLILDDGGDLSAIVHTERKDYLEGLKGISEETTTGVVRLRALERDGL
                     LKVPVIAANNAQMKYLFDNRYGTGQSVWDGIMRTTNLTICGKVVVVAGYGWCGKGVAG
                     KARGLGARVIVTEIDPIKALEALMDGFDVMPMDEAAPLGDIFVTVTGNIDVVSKRHFD
                     LLKDGAVLTNAGHFDVEIAVAALEAYAQNTWEAKPNIKGYRLPNGKSVFVIGSGRLVN
                     IAAADGHPIEIMDGSFGVQYLSMLYLWKNRDKLEPKLFDTPAEIDRELAALKLKSLRK
                     SIDTLTEEQTRYMNSW"
     gene            3778..5289
                     /locus_tag="PKM49_04645"
     CDS             3778..5289
                     /locus_tag="PKM49_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82135.1"
                     /translation="MSINRLLVIADDTERVESFVKRVIELGIEVLGYGEGYRSLLEKG
                     YPIQPLFRPEEESRVTEGSDKTLDCASDPYFYILFRLKTESQESTPLFDMLLADFPKL
                     GRVSSALSFPTVEEEETAPSRVALLRNVAKDFRSIATLVDETDFDWVLSSLAECGDIP
                     LQGRRQLSLKVFSEILKFDTQTYNGFSLLFADESRKHLSLEKALLLQYGSNPHQEAYV
                     ATLSGEDHLLGHLHAVKKGNMTTRRIIDLYNGIRFVRELREPSGVYIRHGNPLWVSVT
                     PGEAEYAGLIRVIKNETIMGGVIVINHTLNRQILEHVRRLPVELVAYNRELEAEATSL
                     QTSSIASVYAFDLWKLSEWEYKYLDGCFIVQERDTEVRQDRFRKLKEELFDPVVEREL
                     ILAFQVSKLTRSDSVVLWAGTQTAGIGAGQMLRRDAFDIALSQAKRRGLAIKETVFAT
                     DGAILDTELFTRVLNSGVRGMVLPSVTKTNQQIAALMAESRVPILLSNHRHFT"
     gene            5434..6456
                     /gene="pheS"
                     /locus_tag="PKM49_04650"
     CDS             5434..6456
                     /gene="pheS"
                     /locus_tag="PKM49_04650"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNY82136.1"
                     /translation="MSLLSDLLEELRLLREQSEQAIEAAENTQMLEELRVRFFGKKGT
                     ISQGILKKLGALPPEERPLLGEKANEVKVLLQEAMDRKNRHFLDAEERVFEESIRIDP
                     TMPGARRMVGFEHLITKTQQEIEDIFLSLGYEIAEGPEIENVHYNFEALNTPDWHPAR
                     DDQDTFYIVPDQWLLRTQTSPVQVRTMEKKTPPIALISPGKVYRSDYDATHLPMFHQC
                     EGLLVDVDVSVSHLKGTLEYFIEKIFGEKKKIRLRPSFFPFTEPSFEVDVLWENRYGE
                     TRWLEVLGCGMVDPNVFRSVGYDSEKWTGFAFGMGIERIAMLKYRLTDIRDFIRGYAH
                     FIRQDL"
     gene            6527..8938
                     /gene="pheT"
                     /locus_tag="PKM49_04655"
     CDS             6527..8938
                     /gene="pheT"
                     /locus_tag="PKM49_04655"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNY82137.1"
                     /translation="MRISKKWLNEYIPVNDLSDGEIDGLLSLSGTEIEETLYPWEGLK
                     GVILGTVIETRPHPSHSRLIVCIIQLPGETKRLVTADKTVKTGDRVLVALPGTVLYNG
                     KRIEAHDFGGVLSEGMLCSLEELGLENKSDYVYRLTDEWPEGFLEDTVFEASITSNRP
                     DELGMLGIARELYAVTKGRRPLTMPPADYDILPDEGDSRFRITIAEPEDCYRYCGLIV
                     RDIRPIDSPLWLKRALVASGLRSISFVVDVTNYVMLETGHPVHAFDLTRLENFRIHVR
                     KAKVGERFVLLDGQSITAEGFECLITDGDNPLALGGIMGGEGSGIEETTKDVLLEVAT
                     FNPVLIRKTRKWHNLNTDASYRFERGVDPNNNPFVLGRLAHLIQKYAGGRVTTEVIDA
                     YPHRIENKTVLLRRSRVESILGASYPSKAIIESLENLGIETEAIEDTEIFRCSIPTGR
                     PDLEREIDLIEEVGRIQGYEQLPGTLPIVRAPDRGRTPYQVFRDDIHRLALSLGYNEI
                     KPLSFTDPADFEPFGIDQSHPWKKEAVALVKPLSRDLSLLRPTLSFGLIKTLAFNFSR
                     QCGDLKVYEIGKVFRRSESFEESEFFGFAHMGAENPEDYTDKRRVGFYAFKGVVEELI
                     HHLSASSDALTFAAPDEGDLYNGFLYPTHSAVISFRGQRIGIIGLVKKHVLNHYDIKT
                     DVYLGEINLNLLFGFIKGSSREWKSVQNLNYPSSRKDFSVLVPRGKEMGRVINGLKRI
                     EYVESVKIIDIYKGKNIPEGFVSVTISLVLRSTESTITEAQMNGVLEKVRAFLIEEKL
                     EIREL"
     gene            8959..9351
                     /gene="queD"
                     /locus_tag="PKM49_04660"
     CDS             8959..9351
                     /gene="queD"
                     /locus_tag="PKM49_04660"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNY82138.1"
                     /translation="MRVSKVFSFDSAHFLPSYHGKCETLHGHTYRLVVTVAGVPDAEG
                     MVIDFILLKKIVQEEVLSVLDHSLLNDILPQPTAENIAKWVWQRISEKVRRDRAALFE
                     VEVWETPTSYVRVRSEDILESTSRGTPD"
     gene            9551..10882
                     /gene="aspS"
                     /locus_tag="PKM49_04665"
     CDS             9551..10882
                     /gene="aspS"
                     /locus_tag="PKM49_04665"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019869747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HNY82139.1"
                     /translation="MVKMHLTERKLIHRVLASENGERVLIAGFLKRKRILGKKLVFGL
                     IQDSTEEIQAVFSLSDIGQENLDTIAGMPLQSVCAVEGTLQKNTDPTKKSELLVSAFK
                     ALSISEPQLPIDLGPGTETGMDLRLNWRFLDLRDERQRTIMLMCSDFERFSRDFFYRE
                     GLREIHSPKIMGTASESGAEVFTVNYFDTKAYLAQSPQFYKQMAIAAGFDRVFEIAPA
                     YRAEKSYTNRHLTEFVSLDVELGYIEDVEDVMAFEERWIVDVLDRLKEKWGATVERLF
                     GVEIEIPALPFPRIRMEEAIEKVNRSGMSVPKGGDLPSEGERRLGQLIAEERHHPFVF
                     VTHFPFSVRPFYHHKTDPVTSCSYDLLWNGVEITTGAQREHQYEVLKGQLAEKGMKEE
                     PLTDYLNFFRFGCPPHGGFGLGIARFLMALLGLENIREATLLTRDPKRLTP"
     gene            complement(10872..11021)
                     /locus_tag="PKM49_04670"
     CDS             complement(10872..11021)
                     /locus_tag="PKM49_04670"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82140.1"
                     /translation="MIYIPCVATVAAIRKEAGTKWMLFSVVYLFFLAYVVSLAIFQIG
                     RLFMG"
     gene            complement(11018..12961)
                     /gene="feoB"
                     /locus_tag="PKM49_04675"
     CDS             complement(11018..12961)
                     /gene="feoB"
                     /locus_tag="PKM49_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HNY82141.1"
                     /translation="MKGITGQRQRRMRRKHLNRRSNLALIEGVSIDRDKFGRNNERTV
                     ALCGNPNSGKTTIFNALTFSHQHVGNWPGVTVEKKEGILRNGEWLYRVVDLPGTYSLT
                     THSADERVARDYLMKDDPDLVLIIIDQTNFERNMYFALEVLEMKKNAILVLNMKDEAE
                     RMGLTIKTDALSSFLGIPVISVYGKSPESISQLKALIQQSAANPVVPKVIRYDEKIEE
                     LISQLQKHDPDPDDEHARWRAIKIIEGAMDFRKSLSPDSLREIDDRLFSFERDTAQDP
                     DIALAEARYGFIHGLTKEFLQRRTTLQERERITTVIDSWLTHRIWGFPIFLAIMWLMF
                     QFTFTVGGFFSSLLDSAFSWFGATVSGVMGESLLSSFIVDGLISGVGSVISFMPEIFC
                     LFLFISFLGDIGYMSRAAFLMDKLMHLFGLHGKSFIPMILGFGCTIPAIMATRSLESR
                     KDRVLTILVTPLMSCSARLPVYVVFAGVFFKNGAGTVIFSIYLLGILLSIIMARVFKK
                     VLFKGEEALFIMELPPYRMPTAKETLGEAWRKTSSFLKKAGTVIFAAVAIIWVLSTFP
                     EGVPYASPESWIGRIGSFIAPIFAPLGFGFWQASVALVFGFFAKEIIVGALGTMLGGR
                     PSSRRPSRYTSPRSPPTASWSSV"
BASE COUNT         3179 a         3536 c         3759 g         2930 t
ORIGIN      
        1 ttgctctttt tataccgcga taggcttggg gagttatcgc ggggtaacca gcgggctgac
       61 ttcggaagcc cgcgaagtcc cgcgactcgc ttttttgtat tctttacgcc gcgatgactt
      121 cggggtatcg cgccgtcgcg aaaagaagaa gcgcttacgc cgcgatgcca ccacataaac
      181 cttatcgcgt tatcgcgaaa aggagaagcg cttacgccgc gatgcccgtg tggggttatc
      241 gcggggtaac caacgggttg acttcggaag cccacgaagt ctcgcgatcc gcctttttgt
      301 attctttacg ccgcgatgac ttcggggtat cgcgccgtcg cgaaaagaag aagcgcttac
      361 gccgcgatgc caccacataa accttatcgc gttatcgcga aaaggagaag cgcttacgcc
      421 gcgatgcccg tgtggggtta tcgcggggta accaacgggt tgacttcgga agcccacgaa
      481 gtctcgcgat ccgccttttt gtattcttta cgccgcgatg acttcggggt atcgcgccct
      541 cgcgaaaaga agaagcgctt atgccgcgat gccacctctt aacttgaccc acacgtggga
      601 catacgattg aagggagttt gcatatggca aaaaagaaac taaaccgatt ctgggcgctt
      661 atcctgattc tgaaccccat cgcctctctc gcgctgggtt tgtgggtctt ctctttgccc
      721 ggaaccctcg ggttggtacg atgggatagc ttctatcgtc tcttggtcgg gttggtcctt
      781 tcgcttggcc tttgggctcc cgtcttgtgg gtcgtgggcc gcctgtttct gaggaagcgg
      841 aagtggctcg caaagatatg gggagtcggt gtcgccgctc tgagcgccgc catattggct
      901 ttcacattgg gcgcctgggg atacctcatt gcccaaccga tgctgaaaag cggagacaaa
      961 cctcccgtgc tgttggtctc ttcccatagc ggggccgcga tgcccgattt aactctggcc
     1021 gtgtggacca aaactccgca acagctgacc gcctctctgg gctcggaaac agaaccgacg
     1081 cgtagcattc tgtcggactc cgggcgggtt catcgcctgt tatttccaaa gctgcaaccg
     1141 gagcgggtct acacctacca gcttgagggc ggagagtccg tctctttccg ggcgctgccg
     1201 gatatcgccc tcgagggatc aaaaccgatg aaagtggtct tttcgtcgga cgtgcactac
     1261 ggaagctcca aaagtagtgc ggaaaagacc acgcgaatga tggatcaatg gctgagccgc
     1321 aaggacgaga tcgacctctt ctgcctgctc ggggatatca cggagttggg gtttatggat
     1381 tcggattggc aggacgcgtt ggactcgtta tcgccagttt tttcacagat cccgatgatc
     1441 ccggtcatag gcaaccacga tttactgttt caagggtatc ggcactatct ggattacctg
     1501 cacccggaag gccggaccag tttcgaagac ctctaccggc gcgtggatat cggacccgtt
     1561 cacttcttac tgttgaacgc cgtttggggc acggaagacg tctctcgtga gcagctcgcc
     1621 tggttggaag cgcaattagc ctcgattcca aaagaccatt ggaaggtcgt tctttcccac
     1681 gcctactact acgcttcggg gtctacgttc gcaggcgaac cctggtatga caatcgggag
     1741 atgatccgca tttttgggcc gatttttgaa aagcgcgggg tggacctcgt cgttagcggg
     1801 cacaatcacc tgctggaagt cctggaacac gccggcgtga cctatgcggt tgtcggcgta
     1861 atgggagccc ctttggatac catcacatat cgctcccccg cctccgtgtg gtgtcgggat
     1921 gttcacgggt ggatggaggt cacctggacc gcgcgggagg cgaccctcca gtttttccaa
     1981 tgggacggcg taccgctgtt ctcacaaacc ttgaccaagt gatgacatat gccttacggg
     2041 ctcgcgggaa gcccttcgcg gtgttcgcca gtctgtgcag gtgaaaagag cgtccgcttt
     2101 ctgagaagtg ctgcgtgggt ataccatcgg ggtacaccaa aatgggaacg ccgaacttta
     2161 gttcggcatg ttcaaaaaag aagatggtgg tttcgcacag agtacacaga gtacacagag
     2221 aaaacataaa cttggcgttg acgcttgtaa acacctctct tctttgtgcc ctccgtggtc
     2281 tccgtgtgaa ccctgttttt ttaagacatg ccaacttaaa agttggcgtt cccagggcgt
     2341 tcacgaagtg ttctcaagcg tatcgagccg tcgcgaaaag aaaaaacgct tacgccgcga
     2401 tgactacacg gaataaaaat cgtacggtcg attctgacct tgtttctcga taacgcctcg
     2461 caactcgacc cacactgttc attctgattc taagcggatg tgatacaata tccgcaacca
     2521 ccacagaaag gggagatagg gagagatgga tgccttgacc gatctgggag tcaaaaagat
     2581 gagctgggca tggcaatata tgcccattct gaacgaatta caccagaaat acaaggatga
     2641 gaagcccttc gccgggtgta agatggtcgt ctgctgccat ctggaagcga aaaccgcgaa
     2701 cttggcgcga cttttccgtg acaccggcgc acaggtgtgg acgactgggt ccaatccgct
     2761 gtccacgcaa gacgagatcg tcgcggctct ggccaatcac gaaaagatgg tggtccacgc
     2821 caaacacacc gaggacaacg aagccttccg caaaaacctt tataaggcgc tggagctcaa
     2881 gccgaacttg atcttggatg atgggggcga tctcagcgcc atcgtgcata ccgagcgaaa
     2941 agactatctg gaagggttga agggcatctc ggaagaaacg accaccggcg tcgtccggtt
     3001 acgcgccctc gaaagagacg gtctcttgaa agtacccgtg atcgcggcga acaacgcgca
     3061 gatgaaatac ctcttcgata accgatacgg taccgggcaa tccgtgtggg acggcattat
     3121 gagaacgact aacctgacga tctgcggaaa ggtggtcgtc gtcgccggct acgggtggtg
     3181 cgggaagggc gtcgcgggaa aggcgcgcgg tttgggcgcg cgtgtcatcg taaccgagat
     3241 cgatccgatc aaagctctcg aggcgctgat ggacggtttc gacgtgatgc cgatggacga
     3301 agctgccccg ctcggtgaca ttttcgtcac cgtcaccgga aatatcgacg tggtatcgaa
     3361 acggcatttc gaccttctga aagacggcgc ggttttgacg aacgccggcc atttcgacgt
     3421 cgagatcgcg gttgccgctc tcgaagccta cgctcaaaac acctgggagg ccaaaccgaa
     3481 tataaaaggg tatcgacttc cgaacggcaa atcggtcttc gtgatcggaa gcggtcgatt
     3541 ggtcaacatc gccgcagcgg atgggcatcc gatagagata atggacggtt ccttcggcgt
     3601 acagtattta tcgatgctct acctctggaa gaaccgcgac aagctggagc ccaaactgtt
     3661 cgacactccc gccgagatcg atcgggaact ggctgccctg aagctgaaaa gcctgcgcaa
     3721 atccatcgat acgttgacag aagaacaaac gcgctatatg aacagttggt gatctctttg
     3781 agcatcaacc ggttactggt gatcgcggac gataccgaac gcgtggaatc tttcgtaaaa
     3841 cgcgttatag aacttgggat agaggtcctc ggatatggag agggctatcg gagcctactg
     3901 gaaaaggggt atccgatcca gccgctcttt cgtcccgaag aggagtcacg ggtgacggaa
     3961 ggttcggaca aaacgttgga ctgcgcttcg gacccgtatt tttacattct gttccgtttg
     4021 aagaccgaat cacaagaatc aacgccactg ttcgatatgc ttctggcgga ctttccgaaa
     4081 ctcgggcgcg tgagttcggc gttatccttt cccaccgtcg aggaggaaga gacggccccc
     4141 tctcgagtcg cgctcctcag aaacgtcgcc aaagacttcc gcagtatcgc caccctcgtc
     4201 gatgagaccg atttcgactg ggtactctct tctttggccg aatgcggcga catcccctta
     4261 caggggagaa gacagttatc cctaaaggtc ttctcggaga ttctgaagtt cgacacgcag
     4321 acctataatg gcttctcgtt gttgttcgcc gacgagagcc gtaagcacct ctctttggaa
     4381 aaggcgctgt tgttacagta cggctcgaac ccccatcagg aagcctatgt ggcgaccctt
     4441 tccggagaag accatctctt ggggcatctg cacgccgtga agaaggggaa tatgaccacg
     4501 cggcggatca tcgacctcta taacggtatc cgttttgtca gagaattgcg agagccctcc
     4561 ggcgtctaca ttcggcacgg caaccctctt tgggtctcgg tgacgcctgg agaagccgag
     4621 tacgctgggt tgattcgggt tataaaaaac gaaaccatta tgggcggggt gatcgtgatc
     4681 aatcacacgc taaaccgcca aatcctcgag cacgtccgca ggctgcccgt cgaacttgtc
     4741 gcgtacaaca gagagctcga agcggaagcc acttcccttc aaacctcctc catcgcttcc
     4801 gtttacgcct tcgatctgtg gaagctctcc gaatgggagt acaagtacct tgacggttgc
     4861 tttatcgttc aagagcggga taccgaggtt cgacaggatc gttttcggaa actgaaagag
     4921 gagcttttcg atcctgtcgt ggaaagagag ctaatcctgg cttttcaagt gtcgaagctc
     4981 acgcgatcgg attcggtcgt cctgtgggca ggtacgcaaa ctgccgggat cggcgcaggg
     5041 cagatgcttc ggcgcgatgc cttcgatatt gccttgtcgc aagcgaagcg gcgtggcctg
     5101 gccataaagg aaaccgtttt tgcgacggac ggcgctatat tggatacgga gctgttcacg
     5161 cgcgtcctca actcgggggt gcgtgggatg gtccttccct ccgtgacgaa aaccaaccaa
     5221 cagatcgccg cgctgatggc cgaaagccgc gtgccgatac tgttgtccaa tcaccgccat
     5281 tttacgtaaa acagaggtct atagaggaag accttgccgg tcgggcggcg tgataaaatg
     5341 cccggcacct aagaaaaggc gagtgggttg tcatccgcac tcgagttcgt ggggaccttc
     5401 acaggtgccc tttgtctatg aggagagata gagatgagcc ttttatcgga tcttttggaa
     5461 gaattgcggc tactccggga gcagtcagaa caagccatag aagccgctga gaacacccag
     5521 atgctcgaag agttgcgggt tcgtttcttt ggaaagaagg ggacgatcag ccaaggcatc
     5581 ctgaaaaagt taggcgcgct gcctcccgaa gagcgccccc ttttgggaga gaaagccaat
     5641 gaggtgaagg tcctccttca ggaagcgatg gatcggaaaa accgccattt cctggacgcg
     5701 gaagaacgcg tctttgagga gtcgattcgg atcgatccca ctatgccggg cgccaggagg
     5761 atggtcggtt tcgagcattt gatcacgaag acgcagcagg aaatcgaaga catctttctg
     5821 tccttggggt acgagatcgc cgaaggcccg gagatcgaaa acgtccatta caacttcgag
     5881 gcgttgaaca ccccggactg gcaccctgcg cgagacgatc aggacacctt ctacatcgtg
     5941 cccgatcaat ggttgctgcg cacccagact tcaccggtcc aggtgcgtac gatggagaag
     6001 aagacgccgc ccatcgcgct catctcccct ggaaaggtct accgcagcga ctacgacgcc
     6061 acgcatctcc cgatgttcca tcagtgcgaa gggttgctgg tcgacgtcga tgtctcggtg
     6121 agccatttga aaggcacact cgaatacttc atcgaaaaga tcttcggcga aaagaagaag
     6181 atcagactca gacccagctt tttccctttc acggaaccga gcttcgaagt cgacgttctc
     6241 tgggagaaca ggtatgggga aacgcgctgg ctagaagtct tagggtgcgg catggtagac
     6301 ccgaacgtct ttcgttccgt cggttacgac agcgaaaaat ggaccggatt cgcgtttggg
     6361 atggggatcg aaaggatcgc gatgctgaaa taccggctca cggatatacg agatttcatc
     6421 cgaggttacg cgcatttcat ccggcaggac ctataaattc tccctgatat ggcgcaaaca
     6481 cgacacgaga acagagaaac ggactctcta aaaaggagcg atagcgatgc gcatctcgaa
     6541 aaaatggctg aatgaataca tccccgtgaa tgacctgtcc gatggcgaga tcgacggtct
     6601 cctcagcttg agcggtaccg agatagagga gaccctgtac ccgtgggagg ggttgaaggg
     6661 ggtgattctg ggcacggtga tcgaaacccg cccgcatcct tcgcatagtc gtctgatcgt
     6721 ttgtataatc cagttaccgg gcgagaccaa gcgattggtc accgccgata aaaccgtaaa
     6781 aacgggggac cgcgtgctcg tcgcgttacc gggaacagtt ctctacaatg gaaaacggat
     6841 agaggcgcac gactttggcg gcgtcctctc ggaggggatg ctctgctcat tggaggagct
     6901 cgggttagag aacaaatccg actacgtcta tcgccttacc gacgaatggc ctgaagggtt
     6961 cttggaggat acggtttttg aggcgtccat tacctcgaac cggcccgatg agttggggat
     7021 gctggggatc gcgcgagaac tgtacgcggt cacgaaaggc cggcgcccct tgacgatgcc
     7081 gcccgcggac tacgatatcc tcccggacga gggcgacagc cgctttcgca tcacgatcgc
     7141 ggagcccgaa gactgttacc gctattgcgg actgatcgta cgcgatatcc ggcccatcga
     7201 ctcgccgctt tggttgaagc gggcgctggt cgccagcgga cttcgatcga tcagtttcgt
     7261 cgtggacgtg accaactacg tgatgttaga aaccggtcac cccgtccacg ccttcgacct
     7321 cacgagactg gaaaacttcc gcatccacgt ccgaaaggcc aaggtaggcg agcggttcgt
     7381 actattggat ggccaatcga tcacggccga aggcttcgag tgcttgatca ccgacggcga
     7441 caacccgctc gccttaggag gcattatggg tggcgagggt tccggcatcg aggaaacgac
     7501 gaaggatgtc ctgctggagg tcgctacctt caacccggtt ttgattcgca aaacccgaaa
     7561 atggcacaat ctgaacaccg atgcctccta ccggttcgag cgaggggtgg atcccaataa
     7621 caacccattc gtgctcggtc ggctcgcgca tttgatacag aaatacgccg gagggcgcgt
     7681 caccacagaa gtgatcgatg cctatccgca ccggatcgag aataaaaccg tccttctgcg
     7741 gcggtcccgt gtagaaagca tcctcggcgc ctcctaccct tcgaaggcga tcatcgaaag
     7801 cctggagaac cttggaatcg agacggaggc catcgaggat accgagattt ttcgctgctc
     7861 catcccgacg gggcgcccgg atttggaacg agagatcgac ctgattgaag aggtcggaag
     7921 aatacaggga tacgaacagc ttccgggaac gttgccgatt gttcgagccc cagaccgggg
     7981 gcgcacaccc taccaagtat ttagggacga tatccatcgt ctggccctat ctttagggta
     8041 taatgaaatc aaaccgttat cttttaccga tccggcggac ttcgagccct tcggtatcga
     8101 tcaaagccat ccgtggaaga aagaagcggt agcgctagtg aaaccgttgt cacgcgacct
     8161 gtctttgctc cgccccacgt tgtctttcgg gctgatcaag acgctcgcgt tcaacttcag
     8221 taggcaatgc ggcgacctca aagtctatga gattggaaag gtctttcggc gttcggaaag
     8281 tttcgaggaa tcggagtttt ttggattcgc ccatatggga gcggaaaatc ccgaggatta
     8341 tacggataaa cggagagtgg gattttatgc gttcaagggg gtagtggaag agcttatcca
     8401 tcacctgagc gcctcctcgg atgcgttgac cttcgccgcg ccagatgaag gtgacctcta
     8461 taacgggttt ttgtatccga cacattccgc cgtgatttcc ttccgcggtc aaagaattgg
     8521 tataataggc ctggtaaaaa aacacgtctt aaatcattat gatatcaaaa ctgacgtcta
     8581 tctgggggag atcaacctca atctcctctt tggcttcata aagggaagca gcagggagtg
     8641 gaagtctgtg cagaatctga actatccatc cagccggaag gacttttccg tccttgtacc
     8701 acgcgggaaa gagatggggc gcgtgatcaa tggacttaag cggatcgagt acgtggaatc
     8761 cgtgaagatc atcgatatct acaaggggaa gaacatcccc gagggattcg tcagcgtcac
     8821 catttcatta gtcttgcgtt cgacggagag cacgataacc gaggcccaaa tgaacggtgt
     8881 gttggagaaa gtcagagcct tcctgattga agagaaactc gagatcaggg agttataacc
     8941 ccgccgacgg gcatccctat gcgtgtcagc aaagtctttt cttttgacag cgcccatttt
     9001 ttaccctcat accacgggaa atgcgaaacg ctccacggtc atacctatcg cttggtcgtg
     9061 acagtcgccg gcgttcccga tgcggaaggg atggtcatcg acttcatcct gctaaagaaa
     9121 atcgtgcaag aggaagttct gtcggtcctg gaccactcgc tcctcaacga tatcctccca
     9181 cagccgacgg ccgagaacat cgcgaaatgg gtctggcagc ggatctcgga aaaagtacgc
     9241 cgtgaccggg ccgctttgtt tgaggtggag gtctgggaaa ccccgacgag ttacgttcgg
     9301 gtacgttcgg aagatatctt agagtccact tcacgcggca cccccgattg agctcaaaac
     9361 acgggtaaag acgagatttc ggaggaagaa ctttggatac gctaaccctt cttgtgattt
     9421 ttataaagaa ccatccatac cggtggaatg gagatgatga tcgcaacccg cgtcggggcg
     9481 tgttgtgata cgaacgcaac caaacccgac gcttagaagc gtcgggtttt tttatgaaaa
     9541 aggagagggt atggtgaaga tgcatctgac ggaaaggaaa ctgattcata gggttttggc
     9601 tagcgagaac ggcgaacggg ttctcatcgc ggggtttcta aaacggaaac gcattttagg
     9661 aaagaagctg gtattcgggc tcatccagga tagcaccgag gagatccaag cggttttttc
     9721 tctttcagat atcggacaag aaaacctcga taccatcgcc ggaatgcctc tgcaaagcgt
     9781 ctgcgccgtc gaagggacgt tacaaaagaa tacagaccct accaaaaagt cggaactgtt
     9841 ggtgtcggct ttcaaagcgc tatcgataag tgaaccacag ttgccgatcg atttgggtcc
     9901 cgggacggaa accggaatgg atttgcgttt aaactggcga ttccttgacc tgcgggacga
     9961 acgtcagcgg acgattatgc ttatgtgctc cgacttcgag cgcttttcac gcgacttctt
    10021 ctatcgggag ggcttgcggg agatccactc tccgaagatc atgggaaccg cttccgagtc
    10081 gggtgcggag gtctttacgg tcaactactt cgatacaaaa gcctatctcg cccagtctcc
    10141 tcagttctac aagcaaatgg cgatcgccgc agggttcgac cgcgttttcg aaattgcgcc
    10201 cgcctatcgc gctgagaaat cctacacgaa ccggcacctg acggagttcg tctctctaga
    10261 cgtcgagttg gggtacatcg aggatgtcga agacgtgatg gccttcgaag agcgttggat
    10321 cgtcgacgtg ctcgaccgat tgaaagaaaa atggggcgcg acggtagaac ggttgtttgg
    10381 agtcgagatc gaaatccctg ctctcccgtt tccgcgcatc cgtatggaag aggcgatcga
    10441 gaaggtcaat cggtcgggga tgtccgtccc aaaaggcgga gacctccctt ccgaagggga
    10501 acggcgattg ggacagctga tcgccgaaga acgccatcat cccttcgttt tcgtgacgca
    10561 cttcccgttt tccgtcaggc ctttctatca tcataaaacc gacccggtta cctcctgcag
    10621 ctacgacctc ctatggaacg gagtcgagat cactacgggg gctcagcggg aacaccagta
    10681 cgaagttttg aaaggtcaac tggcggaaaa aggtatgaaa gaagagccgt taaccgatta
    10741 tctgaacttc tttcgcttcg gatgtccccc gcacgggggt ttcggtttgg gaatcgctcg
    10801 ttttttgatg gcgctgttgg gactagagaa tatccgggaa gccacgcttc taacccggga
    10861 tccgaaacgc ctcaccccat aaacagtcgc ccgatttgaa atatggccaa agagacgacg
    10921 taggcaagga agaagaggta aacgacagaa aacaacatcc atttggttcc ggcctctttc
    10981 cggatcgcgg ctacggtcgc cacgcacggg atatagatca aacagaagac caagaagctg
    11041 taggcggtga gcgaggtgaa gtatagcggg agggccgtcg cgaggagggc ctccccccca
    11101 gcatcgtgcc cagcgccccg acgatgatct ctttcgcgaa gaacccgaag accagcgcga
    11161 ccgacgcttg ccaaaatccg aaaccaagcg gcgcaaatat gggcgcgatg aagctcccga
    11221 tccgtccgat ccaggattcc ggagaggcgt agggcacgcc ctccgggaag gtcgagagca
    11281 cccaaataat cgccacggct gcaaaaatca ccgtcccggc tttcttcaag aaggagctcg
    11341 tctttcgcca tgcttctccc agcgtctctt tcgcggttgg catccgatac ggagggagtt
    11401 ccataatgaa gagggcctct tctcctttaa acaacacttt tttgaaaacg cgggccataa
    11461 tgatcgatag caggatacct aagagataga tcgaaaagat gaccgtgccg gcgccgtttt
    11521 tgaaaaacac tcccgcgaag accacgtaaa cggggaggcg ggcgctacaa gacatcaaag
    11581 gcgtcacgag gatggtgagg acgcggtctt tgcgcgattc gagggatcgg gtcgccatta
    11641 tcgcggggat ggtacatccg aagcctaaga tcatcgggat aaaggacttc ccgtgcaggc
    11701 caaacaggtg catcagcttg tccataagga aggcggcgcg ggacatatac ccgatatctc
    11761 ccaaaaagga gatgaagagg aagaggcaga agatctcggg cataaaggag atgacagagc
    11821 ccaccccgga gatgaggcca tcaacgatga aggagctcaa gagcgactcc cccatcacgc
    11881 ccgaaaccgt agcgccgaac caactgaagg cgctgtcgag aagagaagaa aaaaaaccgc
    11941 cgaccgtgaa ggtgaattga aacatcagcc acataatagc tagaaagatc ggaaaccccc
    12001 aaatgcgatg ggtcaaccaa ctatcgatga ccgttgtgat gcgctctcgt tcttgtaggg
    12061 tcgttcggcg ttgtagaaac tccttcgtca gcccgtggat gaaaccgtac cgcgcctccg
    12121 ccaatgcgat gtccgggtcc tgagccgtat ccctttcgaa agaaaacagc ctgtcgtcga
    12181 tctctctcaa agaatcggga gatagcgatt ttcggaagtc catcgccccc tcgataattt
    12241 tgatcgcgcg ccaacgggcg tgttcatcgt cagggtcggg gtcgtgcttt tgtagctggg
    12301 agatcagttc ctcgatcttc tcgtcgtacc ggatcacctt tggaacgaca ggattcgcgg
    12361 ccgattgctg gatcagcgct ttcaattgag aaatgctctc cggcgatttg ccatagacgc
    12421 tgatcaccgg gatgcccaag aacgaggaga gcgcgtcggt cttgatcgtc aaccccatac
    12481 gctcggcctc atctttcata ttgagaacca atatcgcgtt cttcttcatc tccaacactt
    12541 cgagggcaaa gtacatattc cgctcgaagt tcgtctggtc gataatgatc aacaccaggt
    12601 ccgggtcatc cttcatcaga tagtcccgcg ctacccgctc gtcggcggaa tgggttgtga
    12661 gcgaataggt tcctgggaga tcgacgacgc ggtaaagcca ttcgccgttg cgaagtatcc
    12721 cctctttctt ttctacggtc actccgggcc agttgccgac gtgttgatga ctaaaagtca
    12781 gcgcgttgaa gatcgtcgtt ttcccactgt tcgggttgcc gcacaacgcg accgtgcgtt
    12841 cgttgtttct accgaattta tctctatcta tcgaaacacc ctcgatgagc gccaagttag
    12901 accggcggtt caggtgtttt ctgcgcatac gtctttgcct ttgcccggtt atgcctttca
    12961 tgcaaccctc cgaagccttc gtttcgcgat aaaagatgat tctaccgata ttataacata
    13021 ctacagaatg aacctatatc aataataaat agaccagact actttcgcca agcgacggct
    13081 tatcttcttt gtgtcgtata aaccgacgga ataaccaaga gccctcttca aatactgccg
    13141 gatgtgtgag taaagttatg aggcggcaca actaagaggg tcatcgcggg gtaaccagcg
    13201 ggttgacttc ggaagcccac gaagtctcgc gatacgccgt tcaccccttt tacgaaggct
    13261 cgagagcgcc ctgagaacgc cctgggggcg cttgggaacg ccaaacttta gtttggcatg
    13321 gctccatctt ttcattggga gtgtttccaa gccagcatcg cggcgtaacc cccccttctt
    13381 ttcgtgacgg gcgcgatacg cttt
//
LOCUS       DAOPXN010000057        13191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4800_length_13191_cov_5.070037, whole genome shotgun sequence.
ACCESSION   DAOPXN010000057 DAOPXN010000000
VERSION     DAOPXN010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13191
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4800_length_13191_cov_5.070037"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PKM49_04680"
     CDS             <1..611
                     /locus_tag="PKM49_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82142.1"
                     /translation="AYRLYRGGLVVFSNGPVSYEGLIALRDRVKNEMQALQRTLFGHR
                     EKMGRGPAGTQRVEETPSALTPVELLNGQLAAYGVLLDFRGVFGLNFSPFYPLSGIHL
                     VLVTETLPGEELVKGSDGLRTFLIRRRKDPVNIGDYRIVRGERETETIGAWDPSAAVL
                     RFDGEAFFRLETQLRSSLSSLKTAGVVKIMIELRDKTMEVRL"
     gene            608..1447
                     /locus_tag="PKM49_04685"
     CDS             608..1447
                     /locus_tag="PKM49_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNY82143.1"
                     /translation="MRLEGKPIAESIKVRIKEKAQQLNRQPVLLTVSVAPNESTLTYI
                     RSQEKQAKAQGIAYRHLNFEKEITTEGLLAEIERINRDAEIDAVLLSHPLPKHIDELR
                     LLTALSPEKDVEGRGPVNLGRLAYEKAFLYPCTAEAVMAFIDHYHIPIQGEQVAIVGR
                     STTIGKPLSWMLLEKARNATPIVCHTKTKDIPEIIASSKVVVVATGVPGSLSLSGVRE
                     GAFVIDVGINVVDGKVVGDVSLDPALENERQISVTPTPGGVGPVTSLLLMEHAYICAL
                     GSK"
     gene            complement(1518..2672)
                     /locus_tag="PKM49_04690"
     CDS             complement(1518..2672)
                     /locus_tag="PKM49_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HNY82144.1"
                     /translation="MKKVLFCCLVCLFVGVMGFSQLPLKDVPPNHWAYESVKYLLEAG
                     IISGMTDGTFQGTSNTTRYQLAVSLYRTVEYLKTSLPYLKAENIAGLMTQIDGTKSLV
                     DSLARNVENMGSQVQKVNSQLTAVTNDISELKALRNLIGGWEGRIVALERDVPSLQNS
                     VSTMQGTVYAVQDKVTRAENDMRNVQTETATVKKEINDIRLEISTYGQRIKSVETLVG
                     GISSLELKNSISRVSDDISKVKADQTQLKSSLDAQVIQLADHKKQIDTISQEIPRMKI
                     QIGNVETRISESGMKMDNIAADYEAFKSDILPKVSKNSSDIINLNRKLQDMDVTVNTR
                     LDAVERKITLPTWLGVGGIVLGIAGIGVGVFSLFSLQALIKSLEEESSAP"
     gene            complement(2717..3760)
                     /locus_tag="PKM49_04695"
     CDS             complement(2717..3760)
                     /locus_tag="PKM49_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HNY82145.1"
                     /translation="MYPKLDIYIDKIIHNAQYLRQECQKVGVELTVVTKVIRAMPEIV
                     IPLRDAGVTSFGDSRMINIKRLRQSGIENPLYLIRIPMLSEVPSILEYADFVFVSELE
                     TLKRIVHTSGKKNTAVLFMIDVGDIREGVWFEQADRDLEQALKIAGPRLIGLGTNLGC
                     FGGVLPDENNMRMLCEFGARYGLPTLSGGNSAALLLIEEGTLPEQINHYRVGEAVFLG
                     TDVTRNRAVPGTDLDTFLLRAEIVELQTKPSVPVGEIGQDAFGRKPEFEDKGLRQKAI
                     IAVGEQDIIASGLRPLDPKIIVLHASSDHTILDVTDSDQAYRVGDFLSFRLSYGALLR
                     AMTSEYIFKNIVV"
     gene            complement(3791..5875)
                     /locus_tag="PKM49_04700"
     CDS             complement(3791..5875)
                     /locus_tag="PKM49_04700"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNY82146.1"
                     /translation="MKGIHLLIGVHNHQPVGNFDFIFKEAFQKAYEPFIRTLYKFPNV
                     KMNLHFSGALLLWIAEHSDSLTEMLRKMVQKGQVEMFTGGFYEPVLAVIPEADQRKQI
                     EKLTAFVDRLFGARPKGLWLAERVWEPHLPTVLSQCGIEYVAVDDTHFKNAGFYEDEL
                     NGYFLTEDQGNLLKVFPINKTLRYLIPFDEPEATIRFLQERVSENGDNMYMMFDDGEK
                     FGIWPNTYERTYKEKWLERFFQLLEENRSWLTTSTCQDFVLSKKPKGRAYLPTASYSE
                     MMTWALPSRARNHFERLENDLKAQGNLDRYAMFLKSGFWRTFLSKYTEANLMHKKMLH
                     VRKRITEEMPQEITDHILKAQANDAYWHGLFGGLYLPNLRSSVYENLIQADKKIDEHL
                     HRGQDFIQITVEDIDMDGYPEILINTKKYTIGIAPNCGGRIFEISVKDRNINLCNNLT
                     RRYESYHETMANAQTADEGGKGNLLVKEKGLDKYLNYDWYEKYSCLDHFFGDWNDLTK
                     YSKSRHPEQGDFINQKFDYEITGPETGEVKLTKKGHIWVGSLWLPVRLEKTIQVEDDG
                     FSVHHVITNESDQTIPIMYGCEYNINLLSPQTADRYFYCPEFEKKTLGEAGKVKATFF
                     GARSEYEGVDITFQPGKEVDFWFFPVYTVSFSEGGFEKVYQCSSITPMHHFQLEPLAA
                     YQTNLKIKIQTL"
     gene            complement(5918..7411)
                     /locus_tag="PKM49_04705"
     CDS             complement(5918..7411)
                     /locus_tag="PKM49_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HNY82147.1"
                     /translation="MRWTVIVISNSQIKRILVSTLEDEILLAVLEGQQLVELHFEEID
                     SESITGNIFLSRVENVVNSLDAVFVNIGTSKNAFLRNKDIVKKAYLQQLGGKATDAHR
                     SGAIDSLSKGQKLIVQVKKDPIGSKGPQVTTNIGLTGRFLVYMPYSTTVGVSKKIEDP
                     EERKRLHDFMGGCIHDEGIIIRTVSKDITLATLEEELHTLRVKWQEVLKGFQRFKKPR
                     VLFSEPDILEQLMREKYDTTVDEVITNSKEVYSRLQEILNHFDKKYVKGKLRYCPDDI
                     LELYNVNKQMDDLVKRRIELSCGGSLVIDRTEALTVIDVNSGSNQSNGKSRDMVLETN
                     VQAAKEIARQVRLRNLSGIIIGDFIDMENENDQMTILKVLEEELKKDKAKTSVMGFTK
                     LGLVEMARKRTSPPLGELIHETCPVCKGEGMVFSPKRIVKRMERELEVAAKMKDVKEV
                     LFTVHWNLSGYLSKEWRERSEAAYGKHLVLEFSRQDPNGYDVRYKKE"
     gene            7575..8438
                     /locus_tag="PKM49_04710"
     CDS             7575..8438
                     /locus_tag="PKM49_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82148.1"
                     /translation="MRLFFGILFIVIGVLVFTGSFQWEIFVNAADNFFQAWPFIFVLI
                     GFDVLSKIKGLRWLKYLHALLAISFFCFLFLWPSKNVSEAKTAHMPVDVLLSEEYGDY
                     TIEIKGNAYQFFITESTDTTTNRIIGEIVGHLKDVSVEQYKNVLSIGLDESKASNFFR
                     PENNRLSLTLPHGYSYRVIATGGAINADLALDEVPLSTLDIKGVAILMKATVNPLKTP
                     LLVWLECVFFKGDFVIPDTATWSAKWDSGIKNTDIDKRLKEEYTDPDINFRVNSGILN
                     FGLRGRLKEDR"
     gene            8442..8780
                     /locus_tag="PKM49_04715"
                     /pseudo
     CDS             8442..8780
                     /locus_tag="PKM49_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009374189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            9129..11213
                     /locus_tag="PKM49_04720"
     CDS             9129..11213
                     /locus_tag="PKM49_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNY82149.1"
                     /translation="MKNLIGVLLAICFFFFLFFLMLALTTPNGPIIKDWQYIGPFSAY
                     EAQFPLQIEQRGPFSFRLRSEIRRAQESYLILYNVYASRIDLVRDGVVIDSMGQLEDD
                     AHFWSALPIPFQFGEEGRGRTERIELVVYSREKAAIGRAPFIVAKQPYQRLSGILQVF
                     SYDMFLISIGICFFISFMLVLMGKLTDSRTTYIAIGMSAFFNGLYLLGYVFPDSSAST
                     GLSKVFTILQPITLVVCSYLICVGIESFLTEKISLAKKILWINVFGIFGYSLLFFVPI
                     RHIQIFNLYLWEIPYLTLLLINMVVLLVVSFRSPHGARFIVSVTMFELTVAYELIRVF
                     FLGLPQIPLIGYGSLTVILGYGLIFVWEFQKTYLELQTTSNQLFASYEEMTAMNEELE
                     NSYHELDKKVEQRTHELKVAMNAIKLLMDNTDEGFLSLGPDLTVEENYSSECEVIFGP
                     DFTKESFENLLYPGNEELRAFIKTIIPKILAEETPSTRAMYLSLLPEEVTVRDRIIQM
                     EYRVLESVEDLQSDKKMMVILKDITETRILETQIEEERNTLKMVIKVLVNREDFLESI
                     LDFKQFVGADIFHETLPDIYRIVHTFKGTFANFEMKRAVAALHEVENRINENRETLLK
                     MEARELFDYLSQLKLQNCLIEDIEIIKAHLNPDFFDKDRNSSSNRNGSSTSKKKSSQS
                     CRSSIMRSSWWI"
     gene            11204..12289
                     /locus_tag="PKM49_04725"
     CDS             11204..12289
                     /locus_tag="PKM49_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82150.1"
                     /translation="MDLKRLRYIPFSQLLNHYPDYVSRLSQRMGKWINPVQITSDEIL
                     VDKELFKELARSLVHLFRNSVDHGIEAVEERVENGKDEYGSITLLITEEDRTLNLVFT
                     DDGRGIDVSQVLGKAVEKGILTAESVNEASAQERYQMAHTVIFADNLSTTQETTILSG
                     RGVGLASLKKAVEDLSGTIEVHSVPHEGTEFTIRIPLAEEKLEQISPETIMHPVLKNA
                     IQTLKYYGVSLADEEYAYAPVEELELFAVSSFITIGGLFRSTFALSVDQNLAECLWRS
                     FSLSEKEGEESNALEVEDAVGEISNIILGNSIRSMGIRDTGFAIFPPFTMRAVEATVR
                     LPDNKIWCCQFHTKCGLCKIYFVGIES"
     gene            complement(12265..>13191)
                     /locus_tag="PKM49_04730"
     CDS             complement(12265..>13191)
                     /locus_tag="PKM49_04730"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNY82151.1"
                     /translation="PSRTGERLDRFVTEKTPSWVSRTMVQNAIDRGKVAVNGVIRKSS
                     YRLKAREIVLLDVPTPPVTEILPENIPLSIIHEEEDFLVIDKPPKMMVHPLPTIPGGT
                     LVNALLFHFGDLPQIPGSLTRPGIVHRLDKETSGALVVAKTMIGLQSLCEQFRERKNR
                     KEYLALLHGILPHKSGTVDLALGRHPKSRLKMRIDPSGKEATTDYRVISEFINRVSLA
                     RIRIKTGRTHQIRVHFKHLDAPVLGDYLYGYAEEDRVFGVERQMLHAFRLGFFHPRSH
                     RYVEFLAPLPEDFKRALRVLSGREPQDSIPTK"
BASE COUNT         3111 a         3364 c         3303 g         3413 t
ORIGIN      
        1 ccgcctatag gctctaccgg ggcgggctgg ttgtgttttc gaacggcccg gtctcttacg
       61 agggtttaat tgcgttgcgc gatcgcgtga aaaacgagat gcaggccctc caacgtactc
      121 tattcggaca ccgagagaag atggggcgag gacccgcggg aacgcagagg gttgaagaaa
      181 ctccgtcagc gttaaccccc gttgaactgt tgaacggtca actggccgcg tacggggtat
      241 tgctcgactt tcgcggggtg tttggcctaa atttctcccc cttttacccg ttgtcgggaa
      301 ttcacctggt cttggttacc gaaaccctgc ctggtgaaga actcgtgaaa gggagcgacg
      361 gcctaagaac ctttttaatt cgcaggcgga aagaccccgt aaacataggg gactatcgga
      421 tcgtgcgggg cgaaagagag accgaaacca ttggcgcgtg ggatccgtcc gcagcagtcc
      481 tccggttcga cggggaggcg ttttttcggc ttgaaacgca gctgcgaagc tctctctcaa
      541 gcctgaaaac tgcgggtgtc gtcaaaatta tgatagaatt acgagacaaa acgatggagg
      601 ttcgattatg agattagaag gaaaaccgat tgcggaatcg atcaaggtga ggatcaagga
      661 aaaggctcaa cagcttaatc ggcagccagt attgcttaca gtcagcgtgg cgccaaacga
      721 atcaacgctc acctacattc gctcgcagga gaaacaggct aaagcgcagg ggattgctta
      781 tcggcatctg aacttcgaaa aagagattac gaccgaagga ctcttagccg aaatcgaacg
      841 gatcaaccgc gatgctgaaa tcgacgccgt attgctctct catccgctgc ctaagcatat
      901 cgatgagctc cggttgttga cggccttgtc gccagaaaaa gacgtggagg gccgagggcc
      961 ggtcaatctc ggaagactgg cttatgaaaa ggcctttctc tacccttgta cagcggaagc
     1021 cgtgatggct ttcatagacc actaccacat accgatacag ggagaacagg tcgcgattgt
     1081 gggccggagc accacgatag gaaaaccgct gtcttggatg ctgttggaaa aggcaaggaa
     1141 cgcgacgccg atcgtatgtc acacgaaaac gaaggacatt ccggagatca tcgcctcctc
     1201 gaaggtggtc gtcgtcgcca ccggagtccc tggcagtctc tctctttcag gagtacgaga
     1261 gggcgccttc gtcatcgatg tcggaatcaa cgtggtggac gggaaggtcg tcggtgacgt
     1321 ctctctcgat cccgctcttg aaaacgaaag gcagatatcg gttactccga cgccgggcgg
     1381 cgtagggccg gtaaccagcc tgcttttgat ggaacacgcc tatatctgcg ccttgggctc
     1441 gaagtaacaa aacacgagaa gagagcgggt tatcacccgc tctctttgct taaaaaagaa
     1501 cccttcctaa tccgtagtta gggtgcgctt gactcctctt ctagagattt gataagagct
     1561 tgtaagctga agaggctgaa gacccccaca ccgataccgg cgatgcccag aacgatcccg
     1621 ccaacgccca accaagtggg tagggtaatc tttctctcca cggcatccaa ccgagtgtta
     1681 acggttacgt ccatatcttg gagtttgcgg tttaagttga tgatatcgga gctgttcttg
     1741 gaaactttcg gcaaaatatc gcttttgaag gcttcgtagt cggcggcgat attgtccatt
     1801 ttcataccgg attccgaaat acgggtttcg acgttcccga tctgaatctt catccgaggt
     1861 atttcttggg agatcgtgtc gatttgtttc ttgtgatcgg ccagttggat gacttgcgca
     1921 tctaggctcg acttcaactg cgtttgatcg gctttgacct tgctgatgtc atcgctgacg
     1981 cgggatatgg aattcttcag ctccaaactg ctgatccccc cgacgagcgt ttcgacgctt
     2041 tttatccttt ggccataggt ggagatttcc aaacggatat cgttgatctc tttctttacg
     2101 gtcgccgttt ctgtttggac gttgcgcata tcgttttccg ctctcgtcac cttgtcttga
     2161 accgcgtaaa cggtcccctg catcgtactc accgaattct gcagagaggg gacatcccgt
     2221 tctaaggcta cgattcgacc ttcccacccg cctatcaagt tcctaagagc tttcaactcg
     2281 ctgatatcgt tggtcacagc ggtcagctgg gagttgacct tctgcacctg agatcccata
     2341 ttctctacgt tccgggcgag cgaatccacc aaagacttgg tgccgtcgat ctgggtcatc
     2401 aaccccgcga tgttttcggc ttttaagtaa ggcaaagatg tcttcaaata ctcgaccgtc
     2461 cgatacagcg aaacggcaag ctgataccgc gtcgtgttgc tcgttccttg aaaagttccg
     2521 tccgtcatgc cgctaatgat gccggcttcc aaaaggtact tgactgattc ataagcccag
     2581 tggtttgggg ggacatcctt taaaggcaat tgagaaaaac ccataacccc cacaaataag
     2641 cagaccaaac aacaaaataa aactttcttc atcctctttt tccccctttc taaagagtaa
     2701 gtacttgtgt tcctgattat accacaatgt ttttgaaaat atattcggaa gtcatcgctc
     2761 gtaagagcgc cccgtaactc agcctaaaac tgaggaaatc ccctacccga taggcctgat
     2821 cgctatcggt gacgtccagt atggtgtggt cgctgctagc gtgaagtacg atgatttttg
     2881 ggtcgagcgg ccgaagcccg gaggcgatga tgtcttgctc tcccacggcg attatcgctt
     2941 tctggcgaag ccctttgtct tcgaactccg gttttctccc aaaggcatct tggccaatct
     3001 ctcccactgg taccgacggt ttggtttgca gctccacaat ctctgcgcgt agaagaaagg
     3061 tgtcgagatc tgtgccgggt accgctcgat tcctcgtgac atccgtcccc aaaaatacgg
     3121 cctctcccac ccggtaatga ttgatctgtt caggcaaggt tccttcctcg atcaacagca
     3181 acgcggcgga gttcccaccg gagagggtgg gtaaaccgta gcgcgccccg aattcacaga
     3241 gcatccgcat attgttttcg tctggaagga ctcccccgaa gcaacctaaa ttcgtcccga
     3301 gtccgatgag acgtgggccc gctatcttga gggcttgctc caggtctcgg tcggcctgtt
     3361 caaaccatac cccttcccgg atgtctccca cgtcgatcat aaagaggacg gcggtattct
     3421 tctttccact cgtgtgcaca atccgtttga gtgtctcgag ctcagaaacg aagacaaagt
     3481 ccgcatactc caagatcgat gggacctccg agagcatcgg aatgcggatc aggtagagcg
     3541 gattctctat ccccgattgg cgcagtcgct tgatgttgat cattcgagag tctccaaagg
     3601 aggtcacgcc ggcatcccgc aaagggatca cgatctccgg catcgcgcgg atgacttttg
     3661 tgacgacggt caactcaacc ccgacttttt ggcattcttg ccgcagatat tgagcgttat
     3721 ggatgatctt atctatgtag atgtccagct tgggatacac aaaaaaaccc ctttcctcta
     3781 tgagatggcg ttatagcgtt tgtattttga ttttcagatt ggtttggtag gcggcgagag
     3841 gctccagctg aaagtggtgc ataggggtga tgctggaaca ctgataaacc ttttcgaaac
     3901 cgccttccga gaaggagacc gtgtagaccg ggaaaaacca gaaatccact tctttcccag
     3961 gttggaaggt gatatccaca ccctcgtact cgctccgggc gccaaagaac gtcgccttga
     4021 cctttccggc ctctcccaac gtcttttttt cgaactccgg acagtaaaaa tatcgatcgg
     4081 ccgtttgagg ggagaggagg ttgatgttat actcgcatcc gtacataatc gggatcgtct
     4141 ggtctgactc attggtgatg acgtgatgta cagaaaaacc gtcgtcttcc acttgtatgg
     4201 ttttctccaa tcgaaccggc aaccagaggg atcccaccca tatgtgccct ttttttgtga
     4261 gtttgacctc gccggtttcc ggtcccgtga tctcgtaatc gaacttttgg ttgataaagt
     4321 caccttgttc cgggtggcgg ctcttcgagt acttggtcag gtcgttccag tcaccgaaaa
     4381 agtggtcgag gcacgagtac ttttcgtacc aatcgtagtt cagatatttg tccaaccctt
     4441 tttctttgac cagaaggttc ccttttccgc cttcgtcggc agtttgcgcg ttcgccatcg
     4501 tttcatggta agactcgtat cgccgggtca gattattaca gaggttgatg ttccggtctt
     4561 tgactgatat ttcgaagatg cggcccccgc agtttggagc gatacctatc gtgtactttt
     4621 tggtgttgat caggatctcc gggtagccat ccatatcgat gtcttctacg gttatttgga
     4681 tgaaatcttg acctcggtgt aggtgttcgt ctatcttttt gtccgcttgg atcaggttct
     4741 catataccga actgcgtagg ttcgggaggt aaagtccgcc aaataacccg tgccagtagg
     4801 cgtcgttggc ttgcgctttg aggatatggt cggtgatctc ttggggcatc tcttcggtga
     4861 tgcgtttacg cacgtgaagc atctttttgt gcatcaggtt cgcttcggtg tacttggaga
     4921 ggaaggttct ccaaaagcct gatttgagga acatcgcgta gcggtccagg ttgccttgcg
     4981 ccttcaagtc gttttctagg cgctcaaagt ggtttctggc acggctaggt aaagcccagg
     5041 tcatcatctc ggagtaggat gctgtcggaa ggtaggctct ccctttcggt tttttcgata
     5101 gaacgaagtc ctggcaggtc gacgtcgtca accaactccg gttttcttct agaagttgaa
     5161 aaaaccgctc tagccacttc tctttataag tcctttcgta tgtgttcggc caaatcccga
     5221 acttttctcc gtcgtcgaac atcatataca tattgtcccc attttccgaa acacgttctt
     5281 gtaagaaccg tatggtcgcc tccggctcgt cgaagggaat taggtagcgg agggtcttgt
     5341 tgatcgggaa gactttgagg aggttgcctt gatcctcggt taagaagtag ccgttaagct
     5401 cgtcttcgta aaaacccgca ttcttgaaat gggtatcgtc gacggccaca tactcgatcc
     5461 cacattggga gaggactgtg ggcaaatgag gttcccacac tctctctgcg agccacaacc
     5521 ctttcggtct ggccccgaaa agccggtcca cgaaggcggt taacttctct atttgcttgc
     5581 gctggtccgc ctccgggatc actgccaaaa cgggttcata aaatccgccg gtgaacatct
     5641 cgacttggcc tttttggacc atttttctaa gcatttctgt gagggagtca gagtgttcgg
     5701 cgatccataa gagcagggcg ccgctaaagt gaaggttcat cttaacgtta gggaatttat
     5761 ataaagtgcg aataaacggt tcgtaggcct tctgaaaagc ttccttgaag atgaaatcga
     5821 aattccccac gggttggtga ttgtggacgc ctatcaataa gtgtatacct ttcataaacc
     5881 tcctcctctc ttcccgatta tacaatatcg acgacgatca ttcctttttg tatctcacgt
     5941 cgtatccgtt cgggtcctgt ctagaaaact ccaggaccag atgttttccg tacgcggctt
     6001 cagagcgttc tcgccattct ttcgacaggt atccggacag gttccaatgc accgtaaaca
     6061 aaacctcttt gacatccttc atctttgcgg cgacttcgag ctctcgctcc atcctcttaa
     6121 cgattcgttt gggcgagaag accatccctt cgcctttgca aaccggacaa gtctcgtgga
     6181 tcaattctcc gagaggtggg gaggtccttt tcctggccat ttcgactagg cccagtttcg
     6241 taaaccccat cacggaggtc ttcgctttat ctttctttag ctcttcctcc aataccttca
     6301 gtatcgtcat ttgatcgttc tcgttctcca tatcgatgaa atccccgatg atgatgccgc
     6361 tcaggtttcg taggcgtacc tgcctcgcga tctcttttgc cgcttgaacg tttgtttcta
     6421 aaaccatatc cctgcttttc ccgttcgact ggttgctgcc ggagttgaca tcgatgacgg
     6481 tgagcgcttc ggtgcggtcg atcaccagac tcccaccaca gctcaactca atacggcgct
     6541 tgacgagatc gtccatctgt ttgttcacgt tgtacaactc gagaatgtcg tccggacagt
     6601 atcgcagttt acctttgacg tactttttgt cgaaatgatt gagaatctcc tgcaatcgtg
     6661 agtagacctc tttgctattg gtaatgactt cgtcgacagt ggtgtcgtac ttctctcgca
     6721 ttagctgttc caggatatcg ggctccgaga acagtacgcg aggttttttg aaccgctgaa
     6781 agcctttcaa cacctcttgc catttcaccc gcagggtatg gagttcttct tctaaagtcg
     6841 ccagggtaat gtcctttgaa accgtccgga tgatgatgcc ttcatcatgg atacaaccac
     6901 ccataaagtc gtgcaaccgc ttccgctcct cagggtcttc tatttttttc gagaccccga
     6961 cggttgtgga gtagggcata tagactaaaa accgaccggt caatccgatg ttggtcgtca
     7021 cctgagggcc tttggacccg atggggtctt tcttcacttg aacgatcagc ttctgtcctt
     7081 tgctcaaaga atcgatcgct ccgcttctat gggcatccgt cgcttttccg cccaattgct
     7141 gtaggtaagc tttcttcacg atatccttat tccgtaggaa cgcgtttttt gaagtgccga
     7201 tattgacgaa gacggcatcc aaactgttga cgacgttttc cacacgactg aggaaaatat
     7261 tgcctgtgat gctctcgcta tcgatttctt caaaatgtag ttctacgagc tgctgccctt
     7321 ccaaaacggc gagcagtatc tcatcctcta gggtactgac cagtattctt ttaatttgag
     7381 agttcgaaat gacgataaca gtccacctca ttatgcccta agcgcgtcgc tttgggtccg
     7441 cacatactca cgtggtgttt ttcccctatt ataacccaaa tcgactcaat tttaatacgc
     7501 gcgcccgttc gtgatataat cctcctgttc gaagggaaga caccgtcaga tacgttcccg
     7561 gggaggaaag cagcgtgcgt ttgttttttg gcattttgtt catagtgatt ggcgttttgg
     7621 tattcaccgg atccttccaa tgggagatat tcgtgaatgc ggccgacaat ttctttcaag
     7681 cctggccgtt catcttcgtc ctgatcggat tcgacgtttt gtcgaaaata aagggattgc
     7741 gttggctcaa atacctccac gcccttctgg cgatcagctt tttctgcttc ctctttttat
     7801 ggccctcaaa gaacgtctcc gaagcgaaaa ccgcccacat gccggttgat gtgcttttat
     7861 ccgaagaata cggtgattat acgatcgaga tcaaagggaa cgcctatcag ttctttatta
     7921 ccgaatcgac cgatacgacg acgaatagga tcatcgggga gatcgttggg cacctaaagg
     7981 acgtcagcgt cgaacagtac aagaacgttc tttcgatcgg attagatgaa tccaaagcaa
     8041 gcaatttttt ccgaccggag aacaaccggc tttcgttgac gctaccacac ggctactcct
     8101 accgagtgat cgccaccggt ggggcaataa atgccgatct ggctctggat gaggtcccgc
     8161 tgtccacgtt ggacattaaa ggcgtggcga tcttgatgaa ggccaccgtt aatccgttaa
     8221 aaacgcccct actcgtgtgg ctggagtgcg tcttttttaa aggtgacttc gtcatcccag
     8281 acacggccac ttggtccgca aagtgggatt cggggattaa aaatacggat atagacaaaa
     8341 gattaaaaga agaatacacc gatccggata tcaatttcag agtgaactcg ggtatcttga
     8401 actttgggct tcgcggtcgg ttgaaggagg atcgataatc tatgccaacg atcgttatcg
     8461 ccgacgattc gttcttgatg cggaaaaacc ttagaaccat tttttctcaa gccggttacg
     8521 atgtcgtggc cgaagccgga aacgggaaag cggccgtaaa agcctataag gaatacaggc
     8581 cggatattat gacgatggat atcacaatgc ctgtgatgtc cggaatcgac gccgtgaaag
     8641 agatcacatc ggaattccct caagcgaaga ttattatgat caccgctttg gatcaaaagc
     8701 atatggtttt ccaagcgatc agtaacggcg ccaaacacta cattatgaaa cccttcaaca
     8761 taagtcaagt cttgtcggtg gtgaagagcg ttctagaggg agacgaaaca aaaaaaccca
     8821 cacaagagcc ctactcggtg ctcaaggagg aagaccttta catcgtggcc gtcaaagggc
     8881 ctttggagga gaaaaacctg accctattgc aaggaaccgt ccaaggcctc ttgctgaccg
     8941 aacgctgtca gatactcttc gatctttccg aaaacccgtt cgacgagatc gttgccgtaa
     9001 ggttggagga aatgttaaga aagattaagg gaagcgaagg ccattatgcg atcagaaggt
     9061 gagccggccg atcctctttc gccaagccta tgagacgacc caaaaaagat accgaaggag
     9121 cccgcgggat gaagaacctg atcggcgttt tgttggccat ctgttttttc tttttcctct
     9181 tctttttgat gctcgccctg actactccca acggaccgat cataaaagac tggcagtata
     9241 tcgggccctt ctccgcgtac gaggcgcaat tcccgctaca gatcgagcaa cgcggccctt
     9301 tttccttccg actccgttcc gaaatccgcc gcgcccaaga atcatatctc atcctgtaca
     9361 acgtttacgc cagccgtatc gatctggtac gcgacggtgt cgtgatcgat tcgatggggc
     9421 agttggaaga tgatgcccat ttttggagcg ccttacccat accgtttcag ttcggcgaag
     9481 aggggcgcgg gagaacagaa cgcatcgagt tggtcgtgta tagtcgcgaa aaagccgcca
     9541 taggacgggc gccctttata gtagcgaagc agccctatca acggctaagc ggtatattgc
     9601 aggtattctc ctacgatatg tttctcattt ccatcgggat ctgttttttc atcagcttca
     9661 tgctggtctt gatgggaaag ttgaccgatt ctcgcacgac ttatattgcg atcggtatga
     9721 gcgcgttttt caacggcctc tacctcctcg ggtacgtttt tccggattct tccgcgagta
     9781 cgggcctgag taaggtattc acgatattgc agccgatcac cctcgtggtg tgttcttatc
     9841 tgatctgcgt gggtatcgag tcgtttctga cagaaaaaat ctcacttgcg aaaaaaatcc
     9901 tatggatcaa cgtgttcggc atcttcgggt attctttgct ttttttcgta cccatacggc
     9961 acatacaaat cttcaatctc tacttgtggg aaatacccta tctgacgctc ctcttaatca
    10021 atatggtcgt tttattagtc gtctcctttc gttcgcccca cggagcgaga ttcatcgtct
    10081 ccgtaacgat gttcgaattg accgtggctt acgagctgat ccgagtcttc ttcttgggtt
    10141 tgccacagat accgctcatc ggatacgggt cactgacggt gatcttaggg tatgggttaa
    10201 tattcgtttg ggagtttcag aagacctatc tggagctgca aacgacctcc aaccaactct
    10261 ttgcctccta cgaagagatg acggcgatga acgaggagct ggagaactcc tatcatgagc
    10321 tagacaagaa agtcgagcag cgcactcacg agctgaaagt cgcgatgaac gcgataaagc
    10381 tccttatgga caataccgac gagggtttct tgtctctggg accggacctg acggtagagg
    10441 aaaactacag ctctgaatgc gaagtgatct tcgggccgga cttcaccaag gaatcctttg
    10501 aaaacctcct ctaccctgga aacgaggagc tccgagcgtt tataaaaacg atcatcccga
    10561 agatactggc ggaggaaacc ccttcgacga gagcgatgta tctttctctg ttgccggagg
    10621 aagtgaccgt tcgggatcgt atcatccaga tggaataccg tgtcctcgag agcgtcgagg
    10681 atctacaaag cgataaaaag atgatggtga tcctgaaaga catcacggaa actcgcatct
    10741 tggaaacaca gatcgaagaa gagcgcaata ccctcaagat ggtgatcaaa gtcctggtca
    10801 atcgcgaaga ctttctcgaa tcgattctcg atttcaagca attcgtcgga gccgacatct
    10861 ttcacgaaac cttgcccgac atctatcgaa tcgttcacac cttcaaaggc acgtttgcga
    10921 attttgagat gaaacgcgct gtggccgctc ttcacgaggt ggagaaccgg atcaacgaaa
    10981 atcgggagac cctgttgaaa atggaagccc gggagttgtt cgactacttg agccaattga
    11041 aacttcaaaa ttgccttata gaagatatcg agatcatcaa agcgcatttg aacccagact
    11101 tttttgacaa ggatcgcaac tcctcatcga accggaacgg atcgtcaaca tcgaaaaaga
    11161 aatcctcgca gagctgccgg tcatcgatta tgagaagctc ttggtggatt tgaaacgtct
    11221 tcggtacatc ccgttttcgc aactgcttaa tcactacccg gactacgtta gccgcttatc
    11281 ccaaagaatg gggaagtgga tcaacccggt tcagatcacg tccgacgaaa tcttggtcga
    11341 taaagagctc ttcaaggaat tggcgcgtag cctcgtccat ctcttccgta actccgtgga
    11401 ccacggaatc gaagctgtgg aagaacgcgt cgaaaatgga aaagacgaat acgggagcat
    11461 cacgctgctg atcacggagg aagaccgaac cctcaatttg gttttcaccg acgacggaag
    11521 agggatagac gtctcgcaag ttttaggaaa agccgtggaa aagggaatac tgacggctga
    11581 atcggttaac gaagcctccg ctcaagagcg atatcagatg gctcataccg taatttttgc
    11641 cgacaacttg tccacaacac aagaaacgac gattctgtct gggcggggag tggggcttgc
    11701 ttctctaaaa aaagccgtag aagacctttc tgggacgata gaagtccatt ccgttcccca
    11761 cgaaggaacg gaattcacga ttcgaatccc cttggcagag gagaagttag aacagatttc
    11821 tccggagacc attatgcatc cggttcttaa gaacgcgatc cagactctta agtattacgg
    11881 agtttcttta gccgacgaag aatacgccta cgcgccagtc gaagaactcg agcttttcgc
    11941 ggtttcctct ttcattacga tcggcggact gttccgctcc accttcgccc tctctgtgga
    12001 tcaaaacctt gcggagtgcc tctggcggtc tttttcgctc tcagaaaaag agggagaaga
    12061 atcgaacgct ttggaagtcg aagacgccgt gggcgagatt tcgaacatca tcctcgggaa
    12121 ctcgatacga tcgatgggga tacgagacac gggattcgcg atctttcccc cgtttacgat
    12181 gcgtgcggtt gaggcgacag tacgattgcc ggataacaag atttggtgtt gtcagtttca
    12241 cacgaaatgc ggcctgtgca agatctattt tgttggaatc gagtcttgag gttcccgccc
    12301 ggatagaacg cgaagggctc gtttaaaatc ttcgggaagc ggggccagga actccacgta
    12361 acgatggcta cgcggatgga aaaagcctaa acggaacgcg tgtagcatct gcctctcaac
    12421 cccgaacacc cgatcttcct ccgcataccc atagaggtaa tcgcccagaa ccggggcgtc
    12481 gaggtgttta aaatggactc gtatctgatg ggtcctcccc gttttgatcc gaatccgcgc
    12541 caacgatact cggtttatga actcactgat gacacgataa tcggtagtcg cctcttttcc
    12601 agaagggtcg atccgcatct tcaaccgact tttcggatgc cttcctaaag cgagatcgac
    12661 ggtccctgat ttgtgcggga ggatgccgtg caggagcgcc aaatactctt ttcgattttt
    12721 tctttctcgg aactgctcgc agagggattg taagccgatc atcgttttag cgacgaccaa
    12781 cgccccggaa gtctctttgt ctaaacggtg gacgattccc ggtcgagtca agctccccgg
    12841 gatctgtgga aggtcaccaa agtgaaatag aagcgcgttg accaacgtcc cgccgggtat
    12901 cgttggaagc ggatgcacca tcatcttcgg cggcttgtcg atcacgagga agtcctcctc
    12961 ttcgtgaatg atcgacaggg ggatgttttc gggcaggatt tccgtaactg gaggggttgg
    13021 cacatccaat agaacgatct cacgcgcctt caagcgataa gaggacttcc ggatcacgcc
    13081 gttcaccgcg accttaccgc ggtcgatcgc gttctgaacc atcgtcctgg acacccagct
    13141 cggagttttc tccgtgacga aacggtctag ccgttccccc gtccggctag g
//
LOCUS       DAOPXN010000058        13057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4867_length_13057_cov_5.098062, whole genome shotgun sequence.
ACCESSION   DAOPXN010000058 DAOPXN010000000
VERSION     DAOPXN010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4867_length_13057_cov_5.098062"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..607
                     /gene="rsfS"
                     /locus_tag="PKM49_04735"
     CDS             281..607
                     /gene="rsfS"
                     /locus_tag="PKM49_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNY82152.1"
                     /translation="MNALYWNLSEKEAEGICVLDMQKTPIPTDFFVICTANSQTHMAS
                     LRDAAVEFFDEKGIPLIFYDKGKNYDWLVVDAGIIIVHVFSKRGREFFALEDLWVRAE
                     RLQFGG"
     gene            640..2196
                     /gene="fliF"
                     /locus_tag="PKM49_04740"
     CDS             640..2196
                     /gene="fliF"
                     /locus_tag="PKM49_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNY82153.1"
                     /translation="MEFVKKIVDWFKNFPENYWKKFTRSQKIVFLGTAATILVVMLAM
                     VVILSNPNYTLLIGGLNQSQAGSVIQALEEQGVPYRTTAAGSIYIPASYQPDALRMKL
                     FTQGVLGDSSKGFEIFESQPLGATSFDKQVRYQIALQGSLERSITSIDGIDYARIHLT
                     VPKFTYYTRGDDSVTKASVMLGLRPGVSLSPDKVKAIMELVAAAVEGLDYKNVRVVDN
                     FSRLLSDSVSGDESMGFATSRMEIQRLTENYYTSKVRSSLEQVFGYGRVVVISEILLN
                     WETIEKESKEYSPVTRTSGIVESEQLEKEKSVDRGIGAPVGVDSNVPPTYESTIVTAL
                     GEYEREKTIRNYSINEAYEHVLQNKQGEILAKNITVLLDSTAVSTSVNKDVIKTLVAN
                     AINATLTSVEVDFLAFDRSLEQQYESEMQVSARQQKFIQLTIGFLLLISALTLLLYLL
                     LSRLRLRKQRQEVLSRRQRFEQELLASVAEEKLTPEETELVNMMETLFHAAENQPEEV
                     ALLLRLWLNE"
     gene            2237..4042
                     /gene="lepA"
                     /locus_tag="PKM49_04745"
     CDS             2237..4042
                     /gene="lepA"
                     /locus_tag="PKM49_04745"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNY82154.1"
                     /translation="MFSPEKIRNISIIAHIDHGKTTLTDRILELTNSVEARLMKEQFL
                     DSMDLERERGITIKAHPVTVRYTAKDGNTYEINIIDTPGHVDFSYEVSRSLAACEGAL
                     LVVDSTQGVEAQTVANAYLAINNDLEIIPIINKIDLANANIEETRREIEDLLGLDGSD
                     CLLASGKTGQGLPEILEAIVHEIPSPEKKIGDRLEALIFDAHYDKYQGVVVHVRVFSG
                     SVKPGDKIVLMNLGEVLEVSQVGVFSPKMTPTKALSAGNVGYIIAGMKEVRQARVGDT
                     LTSASEPALKPLPGYQEAKPMVFAGMYPGTPEYYEDLRKALEKLKLNDAALVFEPDNS
                     PALGFGFRCGFLGLLHMDIVKERLEREFEMTVILTAPNVILRIAGPDGKQEEVTNPTR
                     FPDDALEVYEPYVRLSIITPVEYMGNLGGFIQQERRGLFHHMENAGKDRIMISYDVPL
                     SELIFDFFDKLKAKTRGFGSMDYEFLEYRKTRMAKLSIILNREPVDALTTVVHESKKE
                     YYARELALKLKNLIPKHQFEIPIQIKSGSRIIARETIKALRKDVIAKCYGGDVTRKMK
                     LLDRQKEGKRKMRDIGSVKVPQEAFLAILRVGEEE"
     gene            4039..4875
                     /gene="truA"
                     /locus_tag="PKM49_04750"
     CDS             4039..4875
                     /gene="truA"
                     /locus_tag="PKM49_04750"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNY82155.1"
                     /translation="MRRVAAALAYDGTGFCGFQLQEGVRTVEEEIERALHRIFKEPMT
                     IDFAGRTDTGVHAVGQVISFFVKYDTMADRDVKNALNANLPSDIYTRRVWTVETNFNP
                     RFAATRRIYHYYVLNTREPDLFSKNRCWWFPYPLDIPRMRAGSRYLEGIHDFSTFMKK
                     DKYEDKNPVRVVFRVRVTPLRRPGLILIRVEGQSFLRRMVRNIVGALIRVGTGQWEPQ
                     KIKEIIMERTRTALNTTAPPEGLYLYSVDFTPVPIQNYLKNAYWSENDLREDLFCSRA
                     DS"
     gene            4854..5150
                     /locus_tag="PKM49_04755"
     CDS             4854..5150
                     /locus_tag="PKM49_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82156.1"
                     /translation="MFESGFIGIILIIAVAGFLVFSASAKKRKEKKQIRLFEDRVHEY
                     IHQQKILNPFTIYRDIESSPDIPREVLRKMNAFEYIDRAVKQAKAEKGDESGIL"
     gene            5131..5856
                     /locus_tag="PKM49_04760"
     CDS             5131..5856
                     /locus_tag="PKM49_04760"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNY82157.1"
                     /translation="MNLASYKNLHSWKITNEEAKELQTQLAGELKFTVLGKLPRLIAG
                     ADSAFIRIAGEEHIITVIVVLSFPELEMVEKKFLTNKVTFPYIPALLSFREGPSFIKT
                     WKRLNYEPEIVFFDGQGIAHPRLLGLAAHLGLWIDRPTIGVAKSRLFGVTEHTPIKKG
                     EWYPLFHPEDRRVIGATVCTKDGAAPLFISQGNYITLEDSIRLVLSCTNKQRLPEPTR
                     LAHLLTERVKKDKKTVGTQDPEE"
     gene            complement(6121..7416)
                     /locus_tag="PKM49_04765"
     CDS             complement(6121..7416)
                     /locus_tag="PKM49_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82158.1"
                     /translation="MRRLKLRSQINTEKPWEVPEAITKLVKLMISDIVKEGEHRFKRN
                     RVYTVEDLMMHLLVSEIQGISLKEHIAKFGENSEGKPYSKQSLQYRLNNFSAEPLWTL
                     LNKIINLYQNDPGTQAKQTVLSDKTCREFSNIVSISKISLRLPKLLPTDQQEKQNTQL
                     TGEQDKSRSGGHLLVMMDFFNQMIVKAAYLERTLTGDTNSVETLLHWIQPGSLIVLDL
                     GFQDTGLFEEIVKKGADFIIPWRKRKGILRKETLNDTPYYNETLGQIKAESEPILTLR
                     RIKSKSLPQEESEESERIFLVSVTDPEKFPVDEVIRCYDPQHSLQRISQQPEHPLCLS
                     YLWSSKPGHIEIQLVVKMIAYQLYNRFRVALANESDVTVPDVSLISCKHLIKAFAAQP
                     DSPKTVGWISRYLEPFETYAAELQPSGPITLDTHELKET"
     gene            complement(8056..9297)
                     /locus_tag="PKM49_04770"
     CDS             complement(8056..9297)
                     /locus_tag="PKM49_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82159.1"
                     /translation="MPVKNVFLSKVNEMVTGMNDPFVEELRSITGPKRVFPMKATATL
                     LGNRFVEKIEKPYEGISRPLFPKEFSYEYHPFHSQISAYGIVFDQQRLLDEAALLFAH
                     IRDSFNDPRSVKEIATDVLMVVFWANLLIGYFEHLVESELYRNKQLQLTTADYPTLCR
                     EEEFRSILTNMKLAYAFTHLSRISAYSRIPNGNPFDYQWVCRYYLRRKIDVLGGSAMD
                     YIGETRWYDGLERLLRALTSKDTYKSVLKQFPLRGIDELHNIPVYLVAGDEIPAFSST
                     IKSAPRTLDDVLRETKEPNQKFFREILHIVEFSFDLSVNLDHDVGFAIESQDGKALLE
                     MYPRTHTQSLRAPFCDMIALPEAALESLREEGREDLIKELIQLGFEMEASGKMILVLS
                     EKIGWQRWNRTLTCIKRILKI"
     gene            complement(9310..10164)
                     /locus_tag="PKM49_04775"
     CDS             complement(9310..10164)
                     /locus_tag="PKM49_04775"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNY82160.1"
                     /translation="MVSYVGVLLSGIAMGIANIIPGISGGTVLVILKVYEKFVTALSS
                     FHLKGKGFIFAPEAWKFLIVLGIGALIGVFGFAPVMAFLLTRFELFTYLFLIGILLSS
                     LLFVLKSLTTTHKAEVLWVGVGILAFSLVFWASSRVAPYAQTGGGNTLRLFLGGLVGA
                     IFMVLPGISGSMMLIVLGLYEDILFAINHHEPLSIVVIGLGVIAGILISAKVIRYCLN
                     RFRNQTYFFVVGLMLASLVPLWKVNSSHTATDIALGFGFLVLGFLLGYPLSKTSPAKQ
                     IENTHLTS"
     gene            complement(10969..12723)
                     /locus_tag="PKM49_04780"
     CDS             complement(10969..12723)
                     /locus_tag="PKM49_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82161.1"
                     /translation="MKRFLIPLLGVILLMSFFPLAVAFKDSFLHDIYGEKSFAGLDNY
                     AYLFSDRGFWLSLGITVIWAVTSTAIMIYGGLSTALFLNKRGALQKLVFFLLFIPWGI
                     PAYIAVPIWRALIHGNSGNSLFRLLTGITINLFTQPVGAFFATLWVGFWMALPFTAFI
                     FVGHLKKIPRDIIDAARIDGANHRWLLSDIILPLMKPAVIAVTVLNLVKFLKDFTVIF
                     LITGGGPPMPQLITERSILGATTTAEVFLYDLFESARDIGIPSAFSFILGLLILIIML
                     LWYKSQRQARHRSTRTLKARPSFGRAPFELSRRGAALGLHFGSLPLVALVSIGTILSS
                     YALIWMSFSKVDTTYIDTFLPRFFTWRNYTDLFVKEGIPRYYANSLFLSLTSALLIPV
                     LTFGASYAMTRISLKKQTTILTIVSLSTVLSGVHSLIPLFVFFRVFQLTDTYFPLVLI
                     YLSHSIPFSILTLKTYLESFPASFHELARLEGMPERSYLWKVLFPLSLSPIATSMIYT
                     FLNAWNGFLAPMIFLNDDAKYPLSVKLYTYVGKVSSGNPRWGLFSAASILNLIIVFAL
                     FSSLRRPLFHSVLAENSS"
     gene            complement(12720..>13057)
                     /locus_tag="PKM49_04785"
     CDS             complement(12720..>13057)
                     /locus_tag="PKM49_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82162.1"
                     /translation="YTGIPLWMLLAYVDDPNYAPHKQDSSIIAYNRDLALKGYNVKIT
                     AMDGYAITLRSEELDMNNDVLIATTKNGEELPEGEWPLILVWQYDSTQIPANIKGVKQ
                     VTSIEVITD"
BASE COUNT         3290 a         3107 c         3566 g         3094 t
ORIGIN      
        1 gttgtttttt cttatttttt ccctttttct ccaaaaccgg tcgaaagtcg ggccaggaat
       61 gcggcgctgc caggtgtgtt gaaactggcg ccttttccac accagatacg acggtccatt
      121 tttcagaaaa taatgttata atctaaaggt atagtagaca acagggccga tcatcggccg
      181 gcttttcggc ataaggacgg cgaagccgac tttctgagga tcgtactaag gcgtctgaaa
      241 aaaggggggt acttctctgg aatcaaatgt tctcgggatt atgaatgcgc tatattggaa
      301 tctcagcgag aaagaggctg agggtatttg cgtgctggat atgcagaaga cgccgatacc
      361 gacggatttt ttcgtcatct gcaccgcgaa ttctcaaacg cacatggctt ctttacggga
      421 tgcagcggta gagttttttg atgagaaggg tatcccgttg atattttacg acaaaggaaa
      481 gaactacgat tggttagttg tcgacgccgg catcatcatc gtccacgtct tctccaagag
      541 gggaagggag tttttcgccc tcgaagacct gtgggttcgc gccgaacgcc ttcaatttgg
      601 cggttgagcg ctttttagac caggaggaag ttgaaggcca tggagttcgt taagaagatc
      661 gtcgattggt ttaagaactt cccggaaaac tactggaaaa aattcacccg ctcacaaaaa
      721 atagtctttt taggaaccgc agcaacgatc ctcgtcgtca tgctggcgat ggtggtgatc
      781 ctgtccaatc ctaactatac gctcttgatc ggcgggctca atcaatccca agccgggagc
      841 gtcatacaag cgctcgaaga gcaaggggtt ccctatcgta cgacggccgc gggcagtatc
      901 tatatccccg cgtcctacca acccgacgcg ctgcgtatga agttgtttac gcaaggcgtt
      961 ttgggagaca gttcgaaagg tttcgaaata ttcgaatcac agccactcgg cgcgacgagc
     1021 ttcgataaac aggtgcgcta tcagatcgct ttgcaggggt ctttggagcg ttcgatcact
     1081 tcgatcgacg ggattgatta cgcgagaata cacctgactg taccgaagtt tacctactac
     1141 acccgcggag acgacagcgt cacgaaggcc tcggtcatgc ttggcctcag gcccggcgta
     1201 tcgctaagcc cggacaaggt gaaagcgatc atggaattgg tggccgccgc ggtggaaggg
     1261 ttggactaca agaacgtccg ggtcgtggac aacttttcta gattactcag cgattccgtt
     1321 tccggagacg agtcgatggg gtttgcgacc agccggatgg agatacagag gttgacggag
     1381 aattactata cctccaaggt aaggagcagt ttggaacaag tattcggcta cgggcgcgtg
     1441 gtcgtgatct ccgaaatcct gttgaactgg gaaacgatcg aaaaggagtc gaaagaatac
     1501 tcccccgtca cccgcacctc aggaatagtc gaaagcgagc agctcgaaaa agagaagtcc
     1561 gtggatcgcg ggatcggcgc accggttggg gtcgattcca acgttcctcc gacctatgag
     1621 tcgacgatcg tgaccgcttt gggcgaatac gagcgagaaa agaccatccg aaactatagc
     1681 ataaatgagg cctatgagca cgttctccaa aacaaacagg gtgaaatact cgcgaagaat
     1741 attacggtgc tgttggattc cactgcggtt tctacgagtg tcaacaaaga tgtgatcaag
     1801 actctcgtgg caaacgccat caacgcgacg ctgacgagcg tggaagtcga ctttttggct
     1861 ttcgatcgat ccttggaaca gcaatacgaa tccgagatgc aagtctctgc tcgccaacag
     1921 aagttcatcc aactcacgat cggctttctt ttactgataa gcgcgttgac gcttctgttg
     1981 tacctcctct tgagccggtt acgacttcgc aagcagcgtc aggaggttct aagccgacga
     2041 cagagattcg agcaggagtt gctcgcttcc gtggcggaag aaaaattgac acccgaagaa
     2101 acggagctgg tcaacatgat ggagacactt ttccacgctg cagaaaacca gcctgaagag
     2161 gtcgctttgc tgttgaggtt atggctgaac gagtaggtca cgccgatagt cattaattat
     2221 cattgggggg tccgttttgt ttagtccaga aaagatccgc aatatcagta ttattgcgca
     2281 tatagaccat ggaaaaacca cgctcaccga tcggattttg gagctgacga attcggtgga
     2341 agctcgttta atgaaagagc agttcctgga ttcgatggat ttagaacgag aacggggcat
     2401 cacgatcaaa gctcacccgg taaccgtccg ttataccgcc aaagacggga atacctacga
     2461 gatcaatatc atcgataccc ccggacacgt ggacttctcc tacgaggtgt cacggtcttt
     2521 ggcggcgtgt gaaggcgctc tgttggtggt tgacagtacg cagggcgtgg aagcgcagac
     2581 ggtcgcgaat gcctatctgg ccatcaacaa cgaccttgaa atcatcccga ttatcaacaa
     2641 aatagacctc gcgaacgcca atatcgaaga aacgcggcgg gagatcgaag acctgttggg
     2701 gcttgacggt tccgattgtc ttttagccag cggtaaaacg gggcaaggcc tcccggagat
     2761 tttggaggcg atcgttcacg aaatcccttc ccctgagaaa aaaataggag accggttgga
     2821 agcgttgatc ttcgacgccc attacgataa ataccagggt gtggtggtcc acgtgcgggt
     2881 tttcagcgga tcggtgaagc caggcgataa aatcgtcctg atgaatttgg gcgaagtctt
     2941 ggaggtctcc caagtaggcg tcttttctcc caaaatgacc cccaccaaag ctctgtcggc
     3001 agggaacgtg ggatacatca tcgcggggat gaaagaggtg aggcaggcgc gggtaggcga
     3061 tactctcaca tccgcttccg aacccgcatt aaagccgctc ccaggatacc aggaagcgaa
     3121 accgatggtc tttgccggta tgtatcccgg gactcccgaa tactatgaag atttgcggaa
     3181 ggcgttagaa aaattgaagc tgaatgacgc cgctttggtt ttcgagcccg acaactctcc
     3241 cgcactggga ttcggctttc gttgcgggtt tttgggctta ctgcatatgg atatcgtcaa
     3301 agagcgcttg gaacgcgagt tcgagatgac cgtcatcttg acggccccca acgtgatctt
     3361 gaggatcgcg ggccccgacg gcaaacagga agaggtgacg aacccgacgc gctttcctga
     3421 cgacgcgttg gaggtctatg agccatacgt tcgattgtcc atcatcactc ccgtggagta
     3481 tatggggaat ctcggggggt tcatccaaca ggaacgccga gggctcttcc atcatatgga
     3541 aaatgccgga aaagaccgga ttatgatctc ctacgacgtt cctttgtccg aactgatctt
     3601 cgactttttc gacaaattga aagcgaaaac ccgaggcttc gggtctatgg actatgaatt
     3661 cttggagtac cggaagaccc gtatggcgaa gctctcgatc atattgaaca gagaaccggt
     3721 ggacgccctc acgacggtcg ttcacgagag caaaaaagag tattacgcgc gggaattggc
     3781 gctcaagctg aaaaacctga tcccgaagca ccaatttgaa ataccgattc agatcaaaag
     3841 cgggagtcgc atcatcgcgc gagaaacgat taaggccctc cggaaagacg tgatcgcgaa
     3901 gtgttatggt ggcgacgtga ctcggaagat gaagttgttg gacaggcaaa aagaaggcaa
     3961 gcgaaagatg agggatatag ggagcgtgaa agtgccacag gaggcgtttt tggcgattct
     4021 tcgagtgggg gaggaagagt gagaagggtt gccgccgcct tagcctacga cggtacgggt
     4081 ttttgcgggt ttcagcttca ggaaggtgtc aggaccgtgg aggaagagat cgaacgcgcc
     4141 cttcatcgca tcttcaaaga accgatgacc atcgatttcg ccggtcgcac cgataccggc
     4201 gttcacgctg tagggcaagt catcagcttt ttcgtgaaat acgacacgat ggcagacaga
     4261 gacgtgaaaa atgccttgaa cgccaacctg ccatcggaca tctatacgag aagagtctgg
     4321 actgtcgaaa cgaacttcaa cccgcggttt gcagcgacgc ggcgtatcta tcactattat
     4381 gtgttgaaca ccagggagcc ggacctgttc tccaaaaacc gttgctggtg gtttccttat
     4441 cctttggaca tcccgaggat gcgcgcgggg tcgaggtatt tagaagggat ccacgatttt
     4501 tcaaccttca tgaaaaaaga caagtacgag gacaagaacc cagtgcgagt ggtatttcgc
     4561 gtacgcgtga cacctctccg tcgtcctggt ttgatcctca tccgggtgga aggccaatcc
     4621 tttttgagaa gaatggtacg caacatcgtc ggggcgttga tacgcgtggg aaccggacag
     4681 tgggagccgc aaaaaatcaa ggagatcata atggaaagaa cccgaacggc cttgaatacg
     4741 acggcgcccc cggagggtct gtatctctat tctgtcgatt tcacaccagt tccgattcaa
     4801 aattacctta agaatgccta ttggtcggag aacgacttaa gggaggattt gttttgttcg
     4861 agagcggatt catagggatc atcctcatca tcgccgtcgc ggggttcctc gtcttctcgg
     4921 cttcggcgaa gaagcgaaaa gaaaagaaac aaatacgcct gttcgaagac cgggtacacg
     4981 aatacatcca ccaacaaaaa atcctgaatc cttttacgat atatcgagat atagagagtt
     5041 ctccggatat cccgagagag gtattgagga aaatgaacgc tttcgaatac atcgatcgag
     5101 cggtaaaaca agcgaaggcc gaaaaggggg atgaatctgg catcctataa aaacctccat
     5161 tcttggaaga tcactaacga agaggcgaaa gagctacaga cacaactcgc tggggagttg
     5221 aagtttacgg ttctagggaa gttacctcga ctgatcgctg gagcggactc tgcctttata
     5281 cggatcgcgg gcgaagagca tatcatcact gtgatcgtcg ttctctcttt tccggagctc
     5341 gaaatggtcg aaaagaaatt tctgacgaat aaggtgacct ttccctatat tcccgccttg
     5401 ctatccttcc gcgaggggcc ttcattcatt aaaacttgga aacgtttgaa ttatgaaccg
     5461 gagatcgtat tctttgacgg tcaggggatc gctcacccgc gccttttggg attggccgct
     5521 catctcgggc tgtggatcga ccgacctacg atcggcgtgg cgaaatcccg tctttttgga
     5581 gtcacggagc acacgccgat caaaaaaggg gagtggtatc cgttgtttca cccggaggac
     5641 cgtcgggtca tcggggcgac ggtttgtacc aaagacggcg ccgctccgct gtttatctcc
     5701 caagggaact atatcacgtt ggaagactcg atccgcctcg ttttgtcttg cacgaacaag
     5761 caacgattgc ccgaaccaac ccgactggcc cacttgttga ccgaaagagt gaagaaggac
     5821 aaaaaaacgg tgggaaccca agaccctgaa gaataagcgt ggcgtttctt cgggaagcgc
     5881 cttcctggga aggtcttcgg ggcgctgcca gagcggtgga cttgagtctg gcaagggttt
     5941 tgtttgggaa cgccgaacaa aagagaaacc tcaaaccatg ccgaacgaga gttcggcgtt
     6001 cccaggtgtt cgacgttccc agggcgcgtt cacagaaggt ctggtgctcg ggagggcgcg
     6061 gaaatacaaa actgcgtctt cgaaaaatac gagagagatt tcgctttgga aaaatggctc
     6121 ttatgtttcc ttgagttcgt gggtatctaa cgttataggg ccggagggtt gtaactcggc
     6181 tgcataagtt tcaaagggtt ccaaataacg ggaaatccac ccgacggttt tcggagagtc
     6241 gggctgcgcg gcgaaagctt ttataagatg tttacagcta atcagagaga cgtccggtac
     6301 agtaacgtcc gattcattgg ctaaagcgac gcgaaaacgg ttgtataatt gataggcgat
     6361 catcttgacg actagctgga tctctatatg tccaggttta ctgctccaca ggtaggacag
     6421 acacaaggga tgctccggtt gttgtgaaat tctttgaaga ctgtgttgag gatcataaca
     6481 tcgtatgacc tcatcgacag ggaatttttc ggggtcagtt acggagacaa ggaatatgcg
     6541 ctcactttct tcgctttctt cttgtggaag gcttttactc ttgatcctgc gtaaagttaa
     6601 tatcggttca gattcggcct ttatttgacc aagggtttcg ttataatacg gggtgtcgtt
     6661 aagcgtttct tttctcagga tacccttcct cttgcgccaa gggatgatga agtccgcgcc
     6721 tttctttacg atttcttcaa acaacccggt atcttgaaat cctaggtcca gcacgatcag
     6781 agagcctggc tgaatccaat gcaggagtgt ttccacggag ttcgtatctc cggttaacgt
     6841 acgttccaga tatgctgctt ttacgatcat ctggttgaag aagtccatca tcaccagtaa
     6901 gtgaccgcca ctcctcgatt tatcctgctc accggttaat tgagtgtttt gcttttcttg
     6961 ctgatcggtt gggaggagct tcggtaagcg caggcttatc ttgcttatcg acacaatgtt
     7021 gctgaactct ctacaggttt tatcactgag cacagtctgt tttgcttggg tccccgggtc
     7081 gttttggtat aggttgatta tcttgttcag tagtgtccat aggggttccg cggaaaagtt
     7141 gttcagccga tattgtaggc tttgtttcga gtaaggcttg ccttccgagt tttccccaaa
     7201 tttcgcgata tgctctttca acgagatgcc ttgtatctcc gaaacgagca ggtgcatcat
     7261 cagatcttcg accgtgtata cgcggttccg tttaaagcgg tgttcccctt ctttcacgat
     7321 atcagaaatc atcagtttta ctagttttgt aatcgcttcg ggcacttccc aaggcttttc
     7381 agtattaatt tgagatctca attttaagcg tctcaataaa agtcaccccc gcgattatcg
     7441 tgtgtcgctt ttcgatttta tcatacggcc tatgtttctc tgttatcatt cgcatatatc
     7501 ttgtgtttag tatttttttg cgagaagagg tatttttctt cgggctctta aggtcggcgg
     7561 ctcacgcgtc ccgataaacg tagaagatac gcgctcttca tcgggggagg ggcttggagc
     7621 aaaatatgca tcagactaat gccgggcgct cccggaaggg tacctcccag aaagccattc
     7681 ttgggcaaac cttaaaagat agaagagcgg ggcttatgtc tgtaatgtcc tttgcttttc
     7741 accgacaaca aaactggaga taatggggtt tcccatagaa ctcacggcgc ttgcagagga
     7801 atgatggaaa agaagatacc ctgaaactta aagggtattg ctccaaactt cggtgttctg
     7861 ggagcctggc taaggtctgg aagccgaact aaagttcggc gttcccaagg ggcattccca
     7921 gcaaagaaat gcttcaaacc ctgccgaact gaagctcggc gttcccaagg ggcgttccca
     7981 ggagtccggc gtacccaaga aagcccccca gagggggatg aaggctgaca cgccctaacc
     8041 gaggactatt acgcgctata tttttaaaat ccgtttaatg caggtgagcg tacggttcca
     8101 ccgttgccag ccgatttttt cgctgagcac caagatcatt ttccccgagg cttccatttc
     8161 gaaacctagc tgaatcaatt ctttgattaa gtcttcgcgg ccctcttctc ttaaactttc
     8221 cagagcggct tcgggaaggg cgatcatatc gcagaaaggg gcgcgcagcg attgcgtatg
     8281 ggtccgcggg tacatttcca gaagggcctt tccatcctgg gactcgatgg cgaaccccac
     8341 atcgtggtct aggttgaccg agaggtcgaa agagaactcc acgatgtgca ggatttctct
     8401 gaagaacttt tggttgggtt cttttgtctc tcgcagcacg tcgtccaacg tccgcggggc
     8461 ggatttgatc gtggaggaga aagccggaat ctcgtctcct gcgacgaggt acacggggat
     8521 attgtgaagt tcatcgatac cgcgtaatgg gaattgcttg agtacgcttt tgtaggtgtc
     8581 tttcgaggtc aaggccctta atagcctttc taatccgtca taccagcgcg tttctccgat
     8641 gtagtccata gcggaaccgc ccagtacatc gatctttctt ctcaggtaat accgacagac
     8701 ccattggtag tcgaacgggt tcccgttcgg aattctcgaa taggcggaga tgcgagagag
     8761 gtgcgtaaaa gcgtaagcga gtttcatatt cgtaaggatg gatcggaact cttcttctcg
     8821 gcaaagggtg ggatagtccg cggttgtcag ctgaagctgt ttgttacggt acagttcgct
     8881 ttctacgagg tgctcgaaat acccgatcag caggttcgcc cagaagacga ccattaggac
     8941 gtccgtcgcg atctctttca cgcttcgagg atcgttaaag gagtcccgta tgtgggcgaa
     9001 cagcaacgcg gcttcatcaa ggagcctttg ctggtcgaac acgataccgt aagcgctgat
     9061 ctgagagtga aaggggtgat attcgtaaga aaattctttc ggaaacagcg gccggctgat
     9121 tccttcatag ggtttttcga tcttttccac gaagcggttt cccaagagcg tcgccgttgc
     9181 cttcattgga aagactcgct tggggcccgt gatggagcgc aactcttcca caaatgggtc
     9241 gttcatcccg gtgaccatct catttacttt gctcaagaac acgtttttta caggcaaagg
     9301 aatcactcct tacgaggtga gatgtgtatt ttctatttgt tttgcaggag acgtttttga
     9361 cagcgggtac ccgagtaaaa aacctagaac caaaaatccg aaacccagcg cgatatccgt
     9421 cgcggtgtgg ctggaattga ccttccacag tggaacgagg gaagcgagca tcaatccgac
     9481 cacaaaaaaa taggtttggt ttctaaaacg gttgaggcaa taacggatca ctttagccga
     9541 gatcaggatc ccggcgatca cgccgagtcc gatgactacg atggataggg gctcgtggtg
     9601 attgatggcg aagaggatgt cttcgtacag tccgagaacg atcagcatca tcgatccgct
     9661 gattccgggc agcaccatga aaatcgcgcc gaccaatccg cccaggaaga gtctcagcgt
     9721 gtttccccct ccggtttgag cgtaaggcgc cacgcgcgat gaggcccaga aaacgagcga
     9781 aaaggcgaga atacccaccc ctacccagag gacttcggct ttatgggttg tcgtaagaga
     9841 cttcaaaacg aacaagagag aagagagtag gatccctatg aggaagaggt aagtgaacag
     9901 ttcaaaacgg gtcagcaaaa acgccatgac cggcgcgaac ccgaaaaccc cgatcagggc
     9961 gccgattcct aaaacgatca gaaacttcca agcttccggc gcgaatatga accccttacc
    10021 tttcaaatga aaagaagaga gagcggtcac gaacttttcg tacactttca gaatcaccaa
    10081 caccgtcccg ccgctgatgc cggggatgat gttcgcgatt cccattgcga tgccgctcaa
    10141 taatacgccc acataggata ccaaacagac gctcctttgg gtttgcggtt taatcttccg
    10201 cctattcccc tgtcttcctc gattataaca caagacaccg ttcaccggat gaagtgggtg
    10261 cgggtataat atcggggtac accaaattgg gaacgccgaa ctttagttcg gcatgttttt
    10321 ttgcttttca gtaagctctc gagagccctt aaacgcttac actgcgatgg ccgcgcgaaa
    10381 gcgcatcgcg ccgtcgcgaa aagaaggtgc gcttacgccg cgatgaccgt gtggggttat
    10441 cgcggggtaa gtggtttttg cgaccccgcg attcgcccta tgaccttctc aaaagcgtat
    10501 cgcgccttcg cgagaagaag aagcgcttac gccgcgatgc tcgcatagga atgctctcag
    10561 tgaaaagata aacccttgcc aaaccaaagt ttggcgctcc aagcccacga agtatcgcga
    10621 tccgctctgc tgtctatctt tttcaagaca agccaactaa aaagtaggcg ttcccaaaag
    10681 acatgccgga ctaaagtccg gcgttcccag ggtgttccca gggtgttccc agcgcgttcg
    10741 caggaatctt ttctctgtgc gctctgtgcc ttctgtgcga acactgtttt atttttagga
    10801 cctgccaact taaaagttgg cgttcccaag cgtatcgcgc cgttgcgaaa agaaggggcg
    10861 cttacgccgc gatgactaca aggacaaaaa tgctgcaatc gattctggcc ttgtttcttg
    10921 attacacctc ataacttgaa acacacgatg aagacccgct tctctgcctt atgacgaatt
    10981 ttccgctaaa acggaatgaa agagggggcg gcgcaatgag gagaacaagg cgaagacgat
    11041 gatgagattg aggatggaag ccgcgctaaa gagcccccat ctggggttgc cagaggagac
    11101 tttgcccacg taggtgtaca acttcacgct gagcgggtat ttcgcgtcgt cgtttagaaa
    11161 tatcatcggc gcgagaaacc cgttccaggc gttgaggaag gtatagatca tagatgtggc
    11221 gatcggtgac aagctcaacg gaaagaggac cttccagagg taggaacgct ccggcatccc
    11281 ttccaatcgc gccagctcgt gaaaagaagc ggggaagctt tcaagatagg ttttcaaggt
    11341 caatatcgaa aagggtatgg agtgggaaag gtagatgagg acgagaggga aataggtgtc
    11401 ggttagttgg aatactcgga aaaacacgaa cagcgggatc aacgaatgga ctccggagag
    11461 aacggtagag agactgacga tggtcaggat ggtcgtctgc tttttgagcg agatgcgcgt
    11521 catcgcgtaa gaggctccga aggtcaagac cgggatcagt aacgcgctcg tgaggctgag
    11581 aaacaaactg ttcgcgtaat aacgagggat gccttctttg acgaagaggt cggtgtagtt
    11641 ccgccaggtg aaaaaacggg gtagaaaggt gtctatatag gtggtatcca ctttcgaaaa
    11701 gctcatccat atcagcgcgt acgagctcag aatcgttccg atagaaacga gagccaccaa
    11761 cgggagggaa ccgaaatgga gacccaaggc ggcccccctt ctcgagagct cgaagggcgc
    11821 tctcccgaag gatgggcggg cttttaaggt tcgagttgac cggtgtcgtg cctgcctttg
    11881 agatttatac cacaggagca taatgatgag aatcagaagt cccaatatga acgaaaaggc
    11941 cgaggggatg ccgatgtctc gggccgattc aaacaggtcg tataggaaga cctctgcggt
    12001 ggtcgtcgcg ccgagaatgc tcctttccgt gatcaactgt ggcatcggcg gcccgccccc
    12061 ggttatgagg aatatgaccg tgaagtcttt taggaattta acgaggttga ggacggtgac
    12121 ggcgatcacg gccggcttca tcagcggtag tatgatatcc gagagcagcc agcggtgatt
    12181 cgcgccgtct attctggcgg cgtcgatgat atcccggggg atctttttca gatgccctac
    12241 gaagatgaac gcggtaaagg gaagggccat ccaaaagccc acccataggg tggcgaagaa
    12301 ggcaccgacg ggctgtgtga acaggttgat ggtgatcccg gtcaacagcc taaataggga
    12361 attgccgctg ttgccgtgga tgagcgctcg ccaaatgggt acggcgatgt aggccggaat
    12421 gccccatgga atgaataaga gaaagaagac gagtttctga agcgcccctc ttttattgag
    12481 gaaaagggcg gtactcaggc caccgtaaat cataatggcc gtgctcgtga ccgcccagat
    12541 caccgtgatc cctaaactca gccagaaccc ccgatcggaa aagaggtaag cgtagttgtc
    12601 gagtccggcg aaggatttct ctccatagat atcgtgtagg aacgaatcct taaaagccac
    12661 tgccaggggg aagaagctca taagcaggat aacccccagc agtgggatta aaaaacgctt
    12721 caatcagtga tgacctctat gctggtaacc tgtttcacgc ctttaatgtt cgcgggaatt
    12781 tgcgtactgt cgtattgcca taccaggatc aaaggccatt cgccttcggg gagttcttcg
    12841 ccatttttgg ttgtcgcgat gagcacgtcg ttgttcatat ccaattcctc agagcggagg
    12901 gtgatcgcgt acccgtccat cgcggtgatt ttgacgttgt atcctttcaa cgcgaggtct
    12961 cggttgtagg cgatgatcga ggagtcctgt ttatgcgggg cataattcgg gtcgtccaca
    13021 taggcaagca gcatccagag gggaataccg gtgtact
//
LOCUS       DAOPXN010000059        12779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_4996_length_12779_cov_4.989704, whole genome shotgun sequence.
ACCESSION   DAOPXN010000059 DAOPXN010000000
VERSION     DAOPXN010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12779
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4996_length_12779_cov_4.989704"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            966..1577
                     /locus_tag="PKM49_04790"
     CDS             966..1577
                     /locus_tag="PKM49_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82163.1"
                     /translation="MKRLTLLLLICLFASVNLFAIRLGILQSNWQGWLQGAYYYGLRV
                     EQTAPGYPAFGVIQPGDIITEALLIPNSQIWNNPYNPYGGFLLQLNPYLPLANQISVP
                     WYQMYGLQYQNTVDQNAFAAFIGNAMYGSNVVLRLFRPSWNAWTYASVILDLQGNGSV
                     WLMQPVSPPQGNQIVVQPANPSQQGQIQVQPQTPGLQIWININ"
     gene            2572..3426
                     /gene="tsf"
                     /locus_tag="PKM49_04795"
     CDS             2572..3426
                     /gene="tsf"
                     /locus_tag="PKM49_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNY82164.1"
                     /translation="MEVSMEQVKELRRASGAGILDCKKALADSQGDMEKAIDLLRKKG
                     VAAAAKRLGRMTNEGIVSVVLSEDGRQATMLSVMCETDFVARTEEFKALAVELTEDFA
                     KTAPVFVGGPVSEQLAGVYKERLTDLVAKIGENIQLGDYCKFKASPTSHLFYYIHHNK
                     KIGVMLELECQKGGEALDRLGKNLTLHIASLAPEYVAKEDVPEEVLAKEKEIYLEQLK
                     ETGKPQNVLEKIAQGKLEKFYEERCLLLQDFALPPNLPITQMLADTSNELSEKVKVVR
                     FFRAKIGE"
     gene            3481..4203
                     /locus_tag="PKM49_04800"
     CDS             3481..4203
                     /locus_tag="PKM49_04800"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNY82165.1"
                     /translation="MYHKILLKLSGEAFSGIGERGFAPQRLQYLVDELKKVIDMGASL
                     GIVVGGGNHFRGSELKELHPLYADQIGMLGTVINALYLKSALDRQGIRSVVYSNIVEL
                     PGVYPIRYDSLHQNIESGVVALFGGGTSNPLFTTDSAAALRAVEMGADLLIKATKVDG
                     IYSRDPKIHPDAKRYDRLSFSEAIRLNLTVMDIGAFSICQRNGIPIHVLDFFQADNLL
                     KAVLEEHIGTLVCPDSLKPTRL"
     gene            4200..4844
                     /gene="tmk"
                     /locus_tag="PKM49_04805"
     CDS             4200..4844
                     /gene="tmk"
                     /locus_tag="PKM49_04805"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNY82166.1"
                     /translation="MKEKGSLFSFEGIDGCGKTTQIYGIRDDLLELGYRVEVFREPGG
                     TALGEELRRIVLHRKDFPICERAEVLLYVTSRAQLVREKVFPLLEQGTIVLLDRYADS
                     SVAYQGYGRHLPVEEIIRLNDFAIEKRYPCKTFFIDISVEEAESRLSGKEKDRLEGEE
                     QEFFHRIRLGYLALAKRFSDRFVILDGEQPPDVLRKIILRSVLEVVGHPKLETR"
     gene            complement(4851..5588)
                     /locus_tag="PKM49_04810"
     CDS             complement(4851..5588)
                     /locus_tag="PKM49_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82167.1"
                     /translation="MMKRYLAVAFACICALTAFALEQEGILTWRKMVRTFAGTTHLHA
                     RISIRMTLQENATTLTQTQYGLELWVRDLRDYRLDILEPDVLAGITFLFRYRENYFSV
                     LDRRTQKRANQRVSLEPVGAVTPDFDLQEIISNFADFLLGLIETYNPLLSLSYHKDAG
                     HHVYTLLIREPAILTIFKKEYPLILIVVDHNERLERIALQNQKTSEALEICFEELTLS
                     AQPISQKQDPFSIRESEYQLIDYIDLR"
     gene            complement(5585..6739)
                     /gene="fliY"
                     /locus_tag="PKM49_04815"
     CDS             complement(5585..6739)
                     /gene="fliY"
                     /locus_tag="PKM49_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch phosphatase FliY"
                     /protein_id="HNY82168.1"
                     /translation="MADSGNFLTQDELDALLGAISGGGENSRPEPAKPVGGSSKLTAE
                     ELDMIGEVGNIIMGSAATALFTIIGKEVQITTPDVRTSRLSEVRKSFKEERLVTILEF
                     HQGLSGLNILVMDKKTALIIADLMMGGTGQDVKGELDELKSSAVGEAMNQMMGTASTA
                     MAGFLKKPIAITPPNVGISDFSRKEVQFPEISTDGDTDAAVVSFRMEIEDLATTELYQ
                     IVPISFVKRLYALFTEGQTPAPPPAPPPKPAPVEPPVQAAPRKEAPQDMGYFPPPPQK
                     HEPVTVQKAEFEDFSSESLVHLPKQLELLYDVPLEITVELGRSRLTLKEILDLSIGSI
                     IELDKLTGEHVDILVNGKVIARGEVVVVEESFGVRVTEIVNPRDRLRTLR"
     gene            complement(6746..7783)
                     /gene="fliM"
                     /locus_tag="PKM49_04820"
     CDS             complement(6746..7783)
                     /gene="fliM"
                     /locus_tag="PKM49_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNY82169.1"
                     /translation="MADTLSQEEIDSLLRAVTKGEVSVEEIKKDEERAKVKSYDFRRP
                     MKFSKDHTRTFQMIHENFARSLSTYLSGRLRFFVDVSLGPIEQITYGEFLKSVPNPTY
                     MAIFTGSTLKGSAILEINQTIIFGMIDRILGGAGMSITKSRSLTEIETNIIRRETMRF
                     LTLLRDAWASVMEFIPEIENIESNPQFVQIAPINDMTLLITLEVTIGTIEGFVNICIP
                     TSVVEPFTEKITHQFWFSAAEKKEKEELTPKIARNLQNAPITLQAILGNTRLSVGELL
                     YLQKGDVLRLDQYHDQKINIAIGDFPKFEGIPGTHRGKKAVKVTGVRHEITEDQEEEF
                     DLSAWIPKKGE"
     gene            complement(7822..8355)
                     /locus_tag="PKM49_04825"
     CDS             complement(7822..8355)
                     /locus_tag="PKM49_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FliL"
                     /protein_id="HNY82170.1"
                     /translation="MPEADDIQTVAEPKVKKLPVNFLGLLIAAVIAAIVSGAVTFVLV
                     RFVLGTNDPGSSQQKATPSAPIEIRVRFIVEGSNKTFMLKGGKNVVVVDMLSFTVGSD
                     GCRESIATRNDQILSSLQDLFIQKETVEMSNPAGLDLIKRQIRDLVNRVTGYIGDKAK
                     FGVIEVYTYIKAIASVQ"
     gene            complement(8362..9237)
                     /locus_tag="PKM49_04830"
     CDS             complement(8362..9237)
                     /locus_tag="PKM49_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HNY82171.1"
                     /translation="MAKKCPECKAGAPFWLTTYGDMITLMLCFFVALLAFSSINPGKF
                     QEVASGISMVFAGSPPSVLMGGRTTVSDPVISSNPGVKEEIVRISQDDSFKGKLSIIE
                     NEEGTLIVLSKMAFFEPFSAKLTADAKTVLEKIGAVVVEHTTNVLEIRGYTDDRTPPE
                     NSIYPSNWHLGAARAASITKYFLEDLKTKRIAERVVDIRNGRFDPDFYYDPRRFAPVS
                     KGDIDILSEVRSMDMEAKNQLELLDYQLKDGEITQEQAKAKLETMKAEYQSAITVLRH
                     EFQRVDILIKREKVR"
     gene            complement(9243..10034)
                     /locus_tag="PKM49_04835"
     CDS             complement(9243..10034)
                     /locus_tag="PKM49_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HNY82172.1"
                     /translation="MDISTLLGLGMGFIVIALGLVSSGDMSVYLGKDSIMSLIIVLGG
                     AIASVFVGFPKAKSMKIIQIIMSTIQQPKLDYVDAIRTLVSFSEKARREGLLSLEGDI
                     SELKDTFMQKGIQLIVDGTDPDLLKSMMEIEIDLLDSDLSMNGKMLESAGAFAPAFGM
                     LGTVMGLIAMLQALNNPDALGPAMAVALITTFYGSLLSNLIFLPMGEKLGMRKDIFVM
                     HRQMILEGVLSIQAGDNPRLLEEKLKTFLPPDARKAYDAAIPSKE"
     gene            complement(10164..10340)
                     /locus_tag="PKM49_04840"
                     /pseudo
     CDS             complement(10164..10340)
                     /locus_tag="PKM49_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589298.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
     gene            complement(10378..10935)
                     /locus_tag="PKM49_04845"
     CDS             complement(10378..10935)
                     /locus_tag="PKM49_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82173.1"
                     /translation="MSMRTMADHLLDIAQNAINAGATSVRLTVEETSRTFRFLVIDNG
                     KGMDPATVERVFDPFYTTREKKIRRFGLGLPFLREAARMTGGDVRLQSEKGKGTEIEV
                     LFRTDHIDCQPVGDLGSVWFSLLSYSPDIRWHILRRYGEEEYEIDSVVFTQMTRSSED
                     FYAPAFLQLMKESLQELEESIREKG"
     gene            complement(10926..11663)
                     /locus_tag="PKM49_04850"
     CDS             complement(10926..11663)
                     /locus_tag="PKM49_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY82174.1"
                     /translation="MKCFKADLHIHTCLSPCADITMTPSAVCERLVRYGIDWIALADH
                     NSTRNVRAFQRALGRNGIKVMPGIEIQTREDVHILGYFPDAQAAESAGGEVERCLPNV
                     PVDPERDGYSLLVDEHDAFLEVVALPFGFPTSLDIDETVRLIRSYGGQPVFAHVFRNL
                     GLFTQMGLLPESARGIPLEVYLLEKRALVQRFCGTVPVTILSSSDAHSPEALGEARME
                     ICCESRNFSEFVKAIHTQEGRSVRLCL"
     gene            complement(11644..11976)
                     /locus_tag="PKM49_04855"
     CDS             complement(11644..11976)
                     /locus_tag="PKM49_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur binding hydrogenase"
                     /protein_id="HNY82175.1"
                     /translation="MRFSQALERINGTVVVGDPELVEVSRGITCDLLSEVMAHQEQAD
                     LWITVQSHSNIVAVALIVGVKVIILANGRDFYPDAIQKARDENVLLVKSPLGGFELSG
                     IFYEMFQG"
     gene            complement(11980..12360)
                     /locus_tag="PKM49_04860"
     CDS             complement(11980..12360)
                     /locus_tag="PKM49_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNY82176.1"
                     /translation="MIHQSNRAPKPVGPYSVGVSYRDLLFISGQIALNPATNEMVTGS
                     LEAETRQVLGNLKNMLEDFGSSLENVLKVTVFLTDMKRFGDFNAIYAEYFKNNPPARS
                     VIQVGALPKGANVEVEAIAIRKGA"
     gene            12440..>12779
                     /locus_tag="PKM49_04865"
     CDS             12440..>12779
                     /locus_tag="PKM49_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82177.1"
                     /translation="MGIISQYAFLGTPEFNSARFFESSAGNAFLRTPDFSPARFFESS
                     AGNAFLGTPNFSSAWFYLFTGNVPVRASRRKRPFFSRRRDTHLCGLFAGLHQYRETSW
                     ASEVSPLVTPR"
BASE COUNT         2953 a         3487 c         3184 g         3155 t
ORIGIN      
        1 ctctgtgctc tccgtgcgaa ccctgttttt caagacaagt acaaaagcgt atcgcgccgt
       61 cgctaaaaga aggggcgctt acgccgcgat aacagcgaag cacatcgcgg ggtaaccagc
      121 gggctgactt cggaagcccg cgaagtctcg cgatacaaac gtattccagc gatccactct
      181 ctccaacctt tctcttttaa gacttgccga accaaaattc gacgtatcca gagcgttacc
      241 tgtgagctct ttgaggaaat cttcgtgttc tctctgtgtt ctctgtgccc tccgtgcgaa
      301 cccctttttc tttcaaaccc tgccaactta aaagttggcg tttccagggt gtacccagag
      361 agacaggccg actggaagtc ggcccacgaa gagaaggggc gtttacgtca cgttgaccac
      421 gaggcccata gcgggggtag tatattatgt ggccccgtgt tccgcccttt gtccctcttt
      481 cgaagctccg cgctaccggg aagtaactcg ttaccgtcga ccccgaggtt tttttcgaaa
      541 cgtgctataa tactctcaaa agcatcccct cagatagagg gagaaaggag gagcgtgtag
      601 agtatgagaa aaggatggaa gtcttttatc ctgagctgcg tggctatact gttgatggtc
      661 ttcatatcgg cggcaacctg ttctattgag ccgactccca ctacgtattc agtgacggtt
      721 aataattccg cgcgaggatc ttatgtggtc ttattagcaa actatgccaa tcaagggacg
      781 gttccagctt accaaactct gacattttac ggttttgccc caggaacatt tctccaggtt
      841 agaaacagtg gcgggtcctt aatggtgttc gaaggaggga ataactttta cagaatctat
      901 tctaacgtga cattcgtcat accctccggc ggaatttggg tcactgctga aagggattga
      961 ggcgtatgaa gagactaacc ctcctacttc taatctgcct cttcgcctcg gtgaacctgt
     1021 tcgcgatccg cctgggcatc ctgcagtcga attggcaggg ctggctgcag ggcgcttatt
     1081 actacggact ccgggtggag cagacggcgc cggggtatcc ggctttcggc gtgatccagc
     1141 ccggcgacat tatcaccgaa gcgctcctta tccccaacag ccagatctgg aacaacccgt
     1201 ataatcccta tggcggattc ctcttgcagc tcaatccgta cctccccttg gcgaaccaaa
     1261 tctcagtgcc ttggtatcag atgtacggat tgcagtacca gaacaccgtc gaccaaaacg
     1321 ccttcgccgc ctttatcggc aacgcgatgt acggatcgaa cgtcgtgctg cggctgttcc
     1381 gtccgagttg gaacgcttgg acctacgcgt ccgtaatttt ggatctccaa gggaacggca
     1441 gcgtatggtt gatgcagccc gtcagcccac cacagggtaa ccagatagtc gttcaacccg
     1501 caaatccgtc gcagcaggga cagatccaag tccagcccca aacacccggg ctccagattt
     1561 ggattaacat caattagtct ttacgtatca cggcttcgcc ggtcatcgga gaatactacg
     1621 gtttcattga gccgcatcgc gagctgtttt cactgctctt gagacggctt ttgtcggtac
     1681 tgtggaattt ttacacggca atcccaggaa ggcacaccaa acacggtttg cgttgcggaa
     1741 ggcgtttcca gtaaaacaaa gaacatgcca aactaaagtt tggcgttccc agggcgcttc
     1801 cagagagtta cctgggtgct ctttgtgaaa atctttgtgt gctctctgtg ttctccgtgc
     1861 tctctgtgct ctctgtgcga acccttgttt tttctatttt tttctcggct aagaacgtgc
     1921 caaagtaaag ttttgcgttc ccagggggct tccagagagt tacctgggtg ctctttgtga
     1981 aaatctttgt gttctttctg tgcgacccct tgtttttaaa gacaggtaca aaagcgtatc
     2041 gcgccgtcgc gaaaagaaga ggcgcttacg ccgcgataac tacgaagcat ttcgcgaggt
     2101 aaccagcggg ctgacttcgg aagcccacga agtctcgcga aactaacgaa gtccagcgtt
     2161 ccgctctctc ctggctttcg ctttttagac atgctaactt taaagttggc gttcccaggg
     2221 cgatccaaga gggcttccag cacatatctc ccctctgtgc tctctgtgcg aacccttgtt
     2281 tatttttgaa ccgtggcaac tttaaagttg gcgtttccgg gacgttccca agcgcttcca
     2341 aaaaacacaa aaacatgcca aactaaagtt tggcgttccc aaagcgttcc tagcaaggtg
     2401 ccataaatgc gttcccagaa tggcgctccc agcgagactc gcctagattc ttccccggca
     2461 tttctcgatg ctctctgtga gcttcgcgcg aaacactcta tcgaaaaaca ccgccgttta
     2521 tagtataata tcgcggactt tttatcactg cgcgcgaaag gatgatggaa gatggaagtg
     2581 tcgatggagc aagtgaaaga attgagaagg gcatcgggag ccggtatttt ggattgcaaa
     2641 aaagccctcg ccgatagcca gggcgatatg gagaaggcca tagatctcct ccgaaagaaa
     2701 ggcgtcgccg cggccgccaa gcggctgggc cgtatgacca acgaagggat cgtttccgtc
     2761 gtgctgtccg aagacggccg gcaagccacg atgttgtcgg tgatgtgcga aaccgatttc
     2821 gtcgcgagaa ccgaggagtt taaagcccta gcggtagaac tgaccgaaga tttcgccaaa
     2881 accgcgcccg tttttgtggg cggaccggtg agtgaacagt tggccggtgt ttataaagaa
     2941 agattaaccg atctcgtggc caaaatcgga gaaaacatac aacttggcga ttactgcaag
     3001 ttcaaagcgt ctccgacctc acacctgttc tattatatcc accataacaa aaagatcggg
     3061 gttatgttgg aattggaatg tcagaagggc ggggaagccc tggaccggtt gggtaaaaac
     3121 ttgacattgc atatcgcttc tttggcaccc gagtacgtgg cgaaagagga tgtcccggaa
     3181 gaggttctcg caaaagagaa ggagatctac ctcgaacagc ttaaagagac ggggaaacct
     3241 cagaatgtgc tcgagaagat cgcccaaggg aagctggaaa agttctatga agagcggtgc
     3301 ttgctgctcc aggacttcgc tcttcctccg aatttaccca ttacccagat gttggctgac
     3361 acctcgaacg aattgagcga aaaggtcaaa gtcgtcaggt ttttccgcgc gaaaattggc
     3421 gaataattct taaaaccccc tccccggggg ttttattttc tcgataaggg aggctttgcg
     3481 ttgtaccaca aaatcctttt aaaactaagc ggagaagctt tttctgggat tggcgaacgc
     3541 ggtttcgctc cgcaaagact gcagtacttg gtggatgaac tcaaaaaagt gatcgatatg
     3601 ggcgcctcct tggggatcgt cgtcggcggc gggaatcatt tccgaggttc cgagttgaaa
     3661 gaactccatc ctctttatgc cgatcaaatc gggatgctgg ggaccgtaat caacgccctc
     3721 tacctcaaaa gcgccttgga tcgccaaggt atccgctccg tcgtatattc gaacatcgtc
     3781 gagctccccg gggtttatcc catacgttat gacagcttac atcaaaacat cgagagcgga
     3841 gtggtggccc ttttcggcgg aggaacctcg aaccccttgt ttactacgga ttcggccgcc
     3901 gcgcttcggg cggtcgaaat gggcgccgat ctgctgatca aagctaccaa agtggacggc
     3961 atttactccc gagaccccaa gattcatccc gatgcgaaac ggtacgaccg cctttccttc
     4021 agcgaagcca tccgactcaa tctgacggtg atggacatag gggccttctc aatatgccag
     4081 cgcaatggca taccgatcca cgtcttggac ttcttccaag cggacaattt actcaaagcg
     4141 gttctcgaag aacatatcgg aaccctcgtc tgcccggatt ccctgaagcc aacgagatta
     4201 tgaaagagaa aggttctctg ttttctttcg aagggatcga cgggtgcggt aaaaccacgc
     4261 aaatctatgg catccgggac gaccttctcg agctcggtta tcgagtggag gttttcagag
     4321 agcctggcgg tacagctttg ggagaagaac tccgccgaat cgtcttgcat cgcaaggact
     4381 tccctatctg tgagcgcgcg gaggttctgc tgtatgtgac gtcgagggcg cagctggtgc
     4441 gcgagaaggt ctttccgttg ctagaacagg ggaccatcgt cctactggac cgatacgccg
     4501 actcctcagt cgcctatcaa ggctatggac gacaccttcc ggtcgaagag atcatcaggc
     4561 tgaacgattt cgcaatcgaa aagcgctacc cctgcaagac cttcttcatc gatatctccg
     4621 tagaagaagc cgaaagtcgg ttgtccggaa aagagaaaga ccggttggag ggggaagagc
     4681 aagagttctt ccatcgcatc cgcttagggt atttggccct ggccaaacgt ttttcagatc
     4741 gattcgttat actggacggg gagcagcctc cggacgtcct gaggaaaatt attttgcggt
     4801 ctgtgctgga ggtagtcggt cacccgaaac tcgagacccg atagtctgtt tcaccgcaaa
     4861 tcgatgtagt cgatgagttg gtattccgat tcccggatgg aaaaggggtc ttgtttctgt
     4921 gagatgggtt gggcgctcag cgtcagctct tcgaaacaga tctcgagcgc ttccgaagtc
     4981 ttctggttct gtaaggcgat tctctccaaa cgctcgttgt gatccacgac gatcaggatc
     5041 agcgggtatt cctttttgaa aatggttaag atggcgggtt ctcggataag cagggtgtag
     5101 acatggtgtc cggcgtcttt gtgataactg agcgacagca ggggattata ggtctcgatc
     5161 aggccgagta aaaaatctgc aaaattgctg atgatttctt gaaggtcgaa atcgggtgta
     5221 acagcgccaa caggctccaa agaaacccgt tgatttgcgc gtttttgcgt gcgtctatcc
     5281 aaaacggaga agtaattctc tcgatagcga aacaggaagg taattccggc taacacatcc
     5341 ggttctagga tgtccaagcg gtagtctcgc aaatcacgga cccataactc caatccgtat
     5401 tgggtctggg tcaaggtggt cgcattctcc tgaagcgtca tacggatgct aatacgcgcg
     5461 tgcaggtgcg tcgtgcccgc gaaggtccga accatctttc gccacgtcaa aatgccctct
     5521 tgttcgaggg caaaggccgt caaggcgcat atacaggcga atgcgacggc gagatagcgc
     5581 ttcatcatcg cagggttctc aaccgatcgc gcggattaac gatttcggtc acccggacgc
     5641 cgaagctttc ttcgaccacg acgacctcgc ctctcgcgat cactttgccg ttgaccaaga
     5701 tatccacgtg ctctcccgtg agcttgtcga gttcgatgat ggacccgatg ctcaagtcga
     5761 ggatttcttt cagagtcagt cgactgcgcc cgagttccac cgttatttcg agcgggacgt
     5821 cgtacagcaa ttccagctgt ttaggcagat gcacgaggct ttctgaagaa aagtcctcga
     5881 actccgcctt ctgtacggtg accggttcgt gtttctgcgg tggcggtgga aaatacccca
     5941 tatcctgcgg ggcttctttc cttggggctg cctgaaccgg aggttcgacg ggcgcgggtt
     6001 ttggcggcgg cgcgggaggc ggagcaggcg tttgcccttc cgtgaacaac gcgtacaggc
     6061 gtttgacgaa cgagatcgga acgatctgat agagctctgt cgtcgccaag tcctcgatct
     6121 ccattcggaa ggaaacgacc gctgcgtcgg tatcaccatc ggtagaaatc tccgggaatt
     6181 gaacttcctt ccgggagaag tcggagatgc cgacgttagg aggggtaatc gcgatcggct
     6241 ttttcaaaaa cccggccatc gcggtggatg cggtccccat catctggttc atcgcttctc
     6301 ccactgcgct ggacttcagc tcgtccaact cgcctttgac atcctgaccg gttccgccca
     6361 tcatcaaatc cgcgatgatc aaagccgttt ttttatccat caccaggatg tttaacccgg
     6421 aaagcccctg atggaattcg aggatggtga ccagacgctc ttccttgaaa gatttacgga
     6481 cctcgctcag tctggaagtt ctcacatcgg gcgtggtgat ttgcacttct ttgccgatga
     6541 ttgtaaacag ggcggtagct gcggagccca tgatgatgtt ccccacttcc cctatcatat
     6601 ccaattcttc ggctgtgagt ttagacgatc cccctaccgg cttagcaggc tccggccgag
     6661 aattctctcc gcctccgcta atggccccta aaagcgcgtc cagttcgtcc tgtgtgagga
     6721 agttcccaga atccgccacg tcgcatcatt ctcccttctt tggaatccaa gcggaaagat
     6781 cgaactcttc ctcttgatcc tccgtgattt catgccgtac gcctgtcact ttcacggcct
     6841 tttttccgcg atgggtgccc ggaatcccct cgaatttcgg aaagtcaccg atagcgatgt
     6901 tgatcttttg atcgtgatac tggtccaagc gcaaaacgtc ccctttttgt aaatacagaa
     6961 gctctcccac actcaaccgg gtatttccca agatggcctg cagggtgatc ggggcatttt
     7021 gaagattacg ggcgatcttc ggggtcagct cttctttttc tttcttttcc gccgccgaaa
     7081 accagaactg atgggtaatc ttctctgtga aaggctccac aacagaagtc ggaatgcaaa
     7141 tgttcacaaa cccctcgatg gtcccgatcg tgacctccaa cgtgatcaac agggtcatat
     7201 cgttgattgg ggctatttgc acgaattggg gattcgattc gatgttttct atttccggga
     7261 tgaactccat gacgctggcc cacgcatctc ttaggagcgt taaaaaccgc atggtttctc
     7321 gtctgatgat atttgtctct atttcggtca aggaacggga cttggttata gacatccccg
     7381 caccgccgag aatccggtcg atcatcccga agatgatcgt ctggttgatc tccaaaatcg
     7441 cgctaccctt gagggtggaa ccggtgaaga tcgccatata cgtggggttt ggaacggact
     7501 ttaagaattc gccataggtg atctgttcga tcgggcccag actgacatcc acgaaaaacc
     7561 gcagtctccc agacaaatag gtggacaacg atcgcgcgaa gttctcgtgg atcatttgga
     7621 aagtccgggt atgatccttc gaaaacttca ttggacgcct aaagtcgtag gatttgacct
     7681 tcgccctttc ttcgtctttc tttatctctt caacgcttac ctctcctttg gtcaccgcgc
     7741 gtaaaagaga atctatttct tcttggctca gcgtgtcggc catcttcgct caccccctcc
     7801 cgaaacggca agttttcgtc ttcactgcac cgaagcgatc gctttgatat acgtatagac
     7861 ctcgatcact ccgaacttgg ctttgtctcc gatatatcct gtcacacggt tgaccaagtc
     7921 ccgaatttgg cgtttgatca ggtcgagtcc cgcggggtta gacatttcca ccgtctcttt
     7981 ctggataaac aaatcctgca gcgaagagag gatttggtca ttgcgtgtgg cgatcgattc
     8041 acgacacccg tcgctgccaa cggtaaaact cagcatatcg accacgacga cgttttttcc
     8101 tcccttgagc ataaaggttt tattagaccc ttccacgatg aatcgaacgc gtatctctat
     8161 cggggcgctc ggtgtcgctt tttgctgact agatcccggg tcgttcgtcc ccaaaacaaa
     8221 tcgcacgagg acaaaggtga cggcccctga aactattgct gcgataaccg ccgctatgag
     8281 caatccgagg aaattgacgg gcagcttctt aactttaggc tctgctaccg tttgtatatc
     8341 gtctgcttct ggcaacgacg ctcacctcac cttttcccgc ttgatcaaaa tatccacccg
     8401 ctgaaactcg tgccggagca cggtgatggc gctttggtat tcggctttca tcgtctcgag
     8461 tttcgctttg gcctgctcct gggtgatctc gccatccttg agttgatagt ccaacagctc
     8521 caattgattc ttcgcttcca tatccatcga acgcacttcc gacagaatat cgatatcgcc
     8581 tttcgagacg ggggcaaacc gtctcggatc gtaataaaaa tctgggtcga atcggccgtt
     8641 acgaatatcg accacccgct cggcgatccg tttcgttttc aagtcttcca aaaagtattt
     8701 cgtgatgctg gcggcccggg cggctcccaa atgccagttt gacggatata tggagttttc
     8761 gggcggagtc cgatcatcgg tgtaaccgcg tatctccaac acgttcgtgg tgtgctccac
     8821 gaccacggcg ccgatctttt ctaacaccgt tttcgcgtcc gccgtcagtt tcgcggagaa
     8881 aggctcaaag aacgccatct tactgagcac gattaaagtg ccctcttcat tttcgatgat
     8941 cgacagtttc cctttgaagc tatcatcctg actgatccga acgatctctt ctttaacccc
     9001 cgggttactc gaaatgactg gatcggaaac cgtcgttctt ccgcccatca aaacgctggg
     9061 cggcgatccg gcaaagacca tactgattcc gctggcgacc tcctgaaact tacccgggtt
     9121 aatcgacgaa aaagccaaca gagcgacgaa aaaacagagc atcagcgtga tcatatcgcc
     9181 ataggtcgtc aaccaaaaag gggcgcccgc tttacactca ggacattttt tcgccattca
     9241 gatcactcct tcgaggggat ggctgcatca taggccttcc gcgcgtccgg aggcaaaaag
     9301 gtcttcagtt tctcttccag aagcctcggg ttgtctccgg cttgtatcga caatacccct
     9361 tccagtatca tctggcggtg catcacaaaa atgtcttttc tcatcccaag cttctctccc
     9421 atcggcaaaa agatgaggtt cgacaagagc gatccataga acgtcgtaat caacgcaacc
     9481 gccatcgcgg gccccaaggc gtccggatta ttcaatgcct ggagcatcgc gatcaaaccc
     9541 ataacagtac ccaacatacc gaaggcgggc gcaaaggctc ccgcgctctc gagcatctta
     9601 ccgttcatcg acaaatccga gtccagcaaa tcgatctcga tttccatcat actttttaac
     9661 agatccgggt cggtgccgtc cacgatcagc tgtatccctt tttgcataaa ggtatccttc
     9721 agttcggaga tatcgccttc caacgacagt agcccctcgc gccgcgcctt ttcggaaaaa
     9781 gataccaaag tgcggatcgc gtcgacatag tccagtttcg gttgctgtat cgtgctcatt
     9841 atgatctgta tgatcttcat ggactttgcc tttggaaagc caacgaagac ggacgcaata
     9901 gcgcctccga gaacgatgat caaagacatg atcgagtctt tccctaaata aacggacata
     9961 tccccgctcg aaaccaaacc cagggcgatg actatgaaac ccattcccag tcctaataac
    10021 gtagaaatat ccaaatgcat ccctccgtca aagagccatc ttaatgccag atcccgttag
    10081 caggcgtcgc aatccgggtc cttcttctcc tgggatagac ctctggtttg agcgatcaac
    10141 tgcgctactg cgcctaaaga agcgaaactc actttccgga aatccaacac cttctgaaac
    10201 acttcttcaa gcgactcttt cacaatgatc ttcttgccgt tgagcaacgt gatgatcgta
    10261 tctggtctgg actcgaccga ttcgatcaaa tcatcgttca gagcgaaggt atctccattg
    10321 agcttggata aataaatcat tctaacaccc tttagatttt tctctctttc cgtcttttta
    10381 tcctttctct cggatcgatt cttccaactc ctgcaaagac tccttcatca gctgtaaaaa
    10441 agcgggagcg taaaaatctt ccgagcttcg cgtcatctgt gtgaaaacga cgctgtctat
    10501 ctcgtactcc tcttccccgt atcgtcgcaa gatgtgccac cggatgtccg gggagtagga
    10561 cagcaatgag aaccagacgc tgccgaggtc gccgaccggt tgacagtcga tatgatccgt
    10621 acggaacagg acctcgatct ccgtgccctt ccctttctca ctctgtaaac ggacgtcgcc
    10681 gcccgtcatt ctagccgctt ctcgtaaaaa cggcagccct aaaccgaatc ggcgtatctt
    10741 cttctctcgc gtggtgtaaa aaggatcaaa aaccctttcg accgtagcgg ggtccatccc
    10801 cttcccatta tctatgacga gaaagcgaaa ggtccgggat gtttcctcaa cggtcaaacg
    10861 gacgcttgtc gccccggcat tgatggcgtt ttgggcgata tcgaggaggt gatcggccat
    10921 cgttctcata gacataggcg aacgctcctt ccttcctggg tgtgtatcgc ttttacaaac
    10981 tcggagaaat ttcgactttc acagcagatc tccatcctgg cctctcccaa agcctctgga
    11041 gaatgggcgt ccgacgaact caagatcgtc acgggaacgg tgccgcagaa tctctgcacg
    11101 agggcgcgtt tctccaacaa atagacctcc agagggatcc ctcttgcgct ttccggaaga
    11161 agccccatct gggtgaacag tcccaagttc cggaaaacgt gcgcaaaaac cggttgacct
    11221 ccgtagcttc tgatcaggcg caccgtctca tcgatgtcca gcgaggtcgg aaagccgaag
    11281 ggtaaggcga cgacctccag gaatgcgtcg tgctcatcga cgagcaacga gtacccgtct
    11341 ctctccggat cgacgggcac gttgggcaaa caccgttcca cctctccccc tgcagactcg
    11401 gcagcttgcg cgtccggaaa atatccgagg atgtggacgt cctctctcgt ctgtatctcg
    11461 attccgggca tcaccttgat gccgtttcga ccgagcgccc gctggaatgc ccgaacattc
    11521 cgtgtggaat tgtgatcggc aagcgcgatc caatcgatcc cgtaacgcac gagtcgctca
    11581 cagacggccg acggggtcat cgtgatgtcg gcacaaggag aaaggcaggt gtggatatgc
    11641 aaatcagcct tgaaacattt cataaaaaat accgctgagt tcgaaccctc ctaaaggact
    11701 tttcacaagc aacacgttct catcccgggc tttttggatg gcgtctgggt aaaaatcccg
    11761 accattggcc aggatgatca ctttcacccc aacgatcagc gccacggcaa cgatgttcga
    11821 gtgcgactgc acggtgatcc acaggtccgc ttgctcttga tgcgccatca cttcgctgag
    11881 caagtcgcac gtgatgcccc gagaaacctc caccagttcc ggatccccga ccaccaccgt
    11941 cccgttgatc cgttccaagg cttggctaaa gcgcatagat caggctcctt tccttatcgc
    12001 gatcgcctct acctctacat tcgcgccttt cggcaaagcg ccgacctgta tcaccgaacg
    12061 agccggcggg ttatttttga agtattcggc gtaaatggca ttgaaatccc cgaacctttt
    12121 catatcggtt agaaaaaccg tgaccttcaa cacattctct agagaagacc caaaatcctc
    12181 caacatattc ttcaaattac ccaggacctg acgcgtttcg gcttcgaggc ttccagtcac
    12241 catctcgttg gtcgccgggt ttaaggcgat ttggcccgaa atgaacaaca ggtctcggta
    12301 ggatacgcca accgaataag gaccaacggg cttcggcgct ctgtttgact gatggatcat
    12361 cttgctcacg acaaatcctc ctccgattat tatgtgtgaa gagattataa catactgatc
    12421 tataaaaacc tggtcgcgtg tgggcattat atcgcagtac gcctttctgg gaacgccgga
    12481 gtttaattcg gcgcgcttct ttgaaagcag cgctggaaac gcttttctga gaacgccgga
    12541 ctttagtccg gcacgctttt ttgaaagcag cgctggaaac gcttttctgg gaacgccgaa
    12601 ctttagttcg gcatggtttt atcttttcac gggaaacgtt ccagtgcgcg catcgcggcg
    12661 taagcgcccc ttcttttcgc gacggcgcga tacgcatttg tgcgggctgt tcgcggggct
    12721 tcaccagtat cgcgagactt cgtgggcttc cgaagtcagc ccgctggtta ccccgcgat
//
LOCUS       DAOPXN010000060        12596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5083_length_12596_cov_5.110438, whole genome shotgun sequence.
ACCESSION   DAOPXN010000060 DAOPXN010000000
VERSION     DAOPXN010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 12596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12596
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5083_length_12596_cov_5.110438"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1098
                     /gene="murQ"
                     /locus_tag="PKM49_04870"
     CDS             199..1098
                     /gene="murQ"
                     /locus_tag="PKM49_04870"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HNY82178.1"
                     /translation="MLENLDTEKRNPKTMDLDQFSIGELLQAMNEEDATIPLAIRDAL
                     KEISQVVATCLSTIENGGKIYYVGAGTSGRLAIIDAVELIPTFNAPADLFIPIMAGGT
                     EAVFRALEGSEDQEASGETEIVSRAVRKTDYIIGVTASGRTPFVKGALKKGKEIGCPT
                     ALICNVRKPEIAPLADTVVRVVTGPEVLTGSTRLKAGTAQKMVLNMISTATMVKMGKV
                     FQNFMIDVVVLNEKLRERAIKIVKESTGESPERAKSELIKANWKAKQAILQIINDMTP
                     EQAREALEKHGGFLRRAMKVKPK"
     gene            1095..2009
                     /locus_tag="PKM49_04875"
     CDS             1095..2009
                     /locus_tag="PKM49_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HNY82179.1"
                     /translation="MKTFLAIDAGGSTLRATWMSGDGNLIRKETLEKNANFLSAGEKE
                     IENILRFLKHKVPMVDALTLSMAGIDGKPEFDKVKALCDTIFPTTACRILPDADGAYY
                     ANFGIEPGVLVICGTGSIVLGKDEEGKRVRSGGWGYLLGDEGSGFWLVKELFRKYLAY
                     CEFLEERKACFSVFEELFPGNPRDAFVYFYQPETRKRTASFANRFLSLSDPYVIELHQ
                     SGLKSLATQVLSVLRRMGLKQGRICLMGGVFSNAQAEDFFRRYLLGKLSALEQEQISI
                     VAGVKNISDAIAQKMVGETTRSLDGGTE"
     gene            2006..2791
                     /locus_tag="PKM49_04880"
     CDS             2006..2791
                     /locus_tag="PKM49_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNY82180.1"
                     /translation="MRIAVLMKQVPDTDDVKLDEEKGTMIREGIGAVINPLDLHAIEA
                     GRQFVEQDGGTLSVFSMGPPQTEESLREAIAMGAHTATLLTDRKFGGADTWATARTLC
                     EILKLEGPFDLILAGEKATDGETGQVGPEVAAMLGIPFSTYVSAIEKKSDGVIVTRTV
                     EDGYETQFLPFPCLLTVLNNLNQPGMPTLAGKKRGHSATLKIVKAADLSIPEDELGLI
                     GSPTRVSKVFYPKIQRKCEVYTLSDIRTGIERIVRELKNRALL"
     gene            2803..3819
                     /locus_tag="PKM49_04885"
     CDS             2803..3819
                     /locus_tag="PKM49_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNY82181.1"
                     /translation="MEKENRVIPRILVISEVKKNHILPVTFELAGKAAELARKIEGAV
                     DVLLFYDRLDEPVDSLFQAGADRALLVEDSRLGLFNQEIAVKIGLEVLRRYPAQIVLA
                     AATTSGRTFLPAMAAILHTGLTADCTGLDIEPETGLLLQTRPAIGGNVMATIKTPTHR
                     PQMATVRPRTFPKPVPVQRKGAVEQLELPESCFSSKIVPLSHERISDDYTNIQDLDVV
                     VTGGKGLKKPENFALLEKLAKLLGGGVGATRPTVEAKWIDYPHQVGLSGKVVSPKFYL
                     AAGVSGAVQHLAGMQTAEFIVAINKDPEAGIFKIADIGLCGDLFEILPELIAALEKER
                     GEGR"
     gene            3816..5231
                     /locus_tag="PKM49_04890"
     CDS             3816..5231
                     /locus_tag="PKM49_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNY82182.1"
                     /translation="MSNAYNRLTEERMGELQALMAYRHYTVDPERLKVYSHDEVSGEK
                     YRALPEIVLYPETTEEIARIMKWANENRIPVTPRGAGTGLSGGAVPVFSGIVLSLERM
                     NRILELDKETLTITVEPGVVTDEIQRAAKSAGLLYAGDPCSSDASFIGGNVAENAGGN
                     KVIKYGATGRHVMGLEVVLPDGQIARFGGKLVKDVTGYDFVSLMVGSEGTLGIMTKIT
                     LRLIPLLKYSAALLVPFEDVETAIAFVPKMMTQAKLLPASIEFMDQASLALTGRFLNS
                     ELPYATAGAHLIIELEENDKGLLFDEYTRIGEMCLKYGALEVYVADNRNTREKIWKAR
                     KAIAEAVSSFYPEHCMEDIVVPINRIPQIVEKVRDISERYRLETINFGHAGDGNLHVT
                     FLNHRYTTEQWEETIENALDDLYRETVLLGGTLSGEHGIGLKRKKYLSTVLSPTVLEL
                     TRRIKNAFDPNGILNPGKIVD"
     gene            5334..5996
                     /locus_tag="PKM49_04895"
     CDS             5334..5996
                     /locus_tag="PKM49_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82183.1"
                     /translation="MKMVRLFVIVILFMCLMTSCTFFKDLFLDVNKIGRDFVTAFDNL
                     VKTLEIPLGPSTGEFENVRSFEGREIDPVLTAFMNTYVHLATGVTKGSEAYEVLELVI
                     AIMTVYSKKVTFVSAEKIDIPNITTGARVISPVWCGNHPAYVSKGILVKMRMNLDIPE
                     WDSDMIVAWPLMLIDNKPYMFTVYKATDTPGEYYPFYPIPFFALPFIQVHSSREAAAT
                     AG"
     gene            6063..6443
                     /locus_tag="PKM49_04900"
     CDS             6063..6443
                     /locus_tag="PKM49_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82184.1"
                     /translation="MKKKTLLGVLVVVLVFSFLFVGCASATYNWLYGVWKIAGWGVFL
                     GDGRSVPSDAAAELNVTKVWPSEEFEGNYGFLDGASKPVNYTIMDAKVVEGKTVRFSV
                     NDNGTVRTFVGELVVGKTAIGTWE"
     gene            6473..7183
                     /locus_tag="PKM49_04905"
     CDS             6473..7183
                     /locus_tag="PKM49_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82185.1"
                     /translation="MKKSLVFLLLALLAISTFSITMGIYVDNFKGAIDGCKVKGVYVN
                     KVEPNYPAYGHICIGDIITEAVLVGEGKVWSHCCELPPTGRTFVFNPDYSLRDQLFML
                     KEARAKYYFNDINRWQDMAYLLNRALPGDTALLRIYRTQERRWLYVPVSLGAEGRYTF
                     MNTPTYQPQVFAQPVAVVPVVAVPVVTYQTVTVLPAFPLSNCTGTVPLQVAPQPIAPA
                     PVVTTVVPSCCSIIIWLP"
     gene            7318..8016
                     /locus_tag="PKM49_04910"
     CDS             7318..8016
                     /locus_tag="PKM49_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HNY82186.1"
                     /translation="MSNEWLAAISIFVDLSLVLVLFRAFRKEGLYLIIVANIIICNIQ
                     VLKTVNLFGLTTTLGNVLYGSIYLTTDILSETYGQKAANKGVWLGFTALLFLTLYTQM
                     ALWFTPHPEDFASPHLSALFSLMPRVALGSLTAYFVSQFLDVWLFHKWKNLTRDRHLW
                     WRNNFATAVSQIADTLIFCSIAFLGLYPIEMWWQIVLTTYIYKFVVALCDTPFVYLGR
                     RMGKKFEQEALTHG"
     gene            8009..8794
                     /locus_tag="PKM49_04915"
     CDS             8009..8794
                     /locus_tag="PKM49_04915"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HNY82187.1"
                     /translation="MDNRIWEVVRRIEESQALVVLTGAGVSTDSGISDFRSPDGLYSR
                     WDRNLVFNIDYFHQDPTYFYRFAKEELYRFDSIEPNVTHRLLALLESEGYLKMLITQN
                     IDGLHQKAGTRAIIELHGGVSQGHCQKCGKAVPAVRIKEAIARAPVARCDDCGGLVKP
                     DVIFFGEALDTSNMENAFYWSRKADVFLVLGSSLQVYPAGYLPEVAKRSGAYLAIVDQ
                     QDTAQDDIADRKLVMPLRDFSEALYGVFSSPDYPKTDRGDRFK"
     gene            9188..>9736
                     /locus_tag="PKM49_04920"
     CDS             9188..>9736
                     /locus_tag="PKM49_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY82188.1"
                     /translation="MIDRLPVFFLQVLGLVEPWYISDVEVRGLEIHIRVDFKRGAKFP
                     YKGELCRVHDTVIREWRHMNLFQYKTYLHARVPRIYTPEGVKQVQVPWAREGSGFTLF
                     FEALLLSLAQLTSVAQVHQLCDVSDNRIWRVLEHYIEKEVKFQDFSEEPVTTLSIDEV
                     ARKKGHVYLTSFMDLERAKVVHV"
     assembly_gap    9737..9836
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9837..10563
                     /locus_tag="PKM49_04925"
     CDS             <9837..10563
                     /locus_tag="PKM49_04925"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HNY82189.1"
                     /translation="HVYLTSFMDLERAKVVHVSDGKGKESIFSFKERYEEKEGKVDDI
                     ETVVMDLSPAFISAIEEAFPKATIVFDKFHVVKLLNNVLDSIRRRERKENIPRFNKIR
                     YLLLKDPKKLKKSQRERLDEFLTQANLDTVKAYNLIQMFKRGYEYQKPSWAGKFFHHW
                     IKLCQQSKIPEMLKGAQAILTHLKGIMAYFTYRVTNAVMEGNNSKLRTITKRSYGFKT
                     LRYLRLMIFLVFGKLRFDVPRLT"
     gene            11081..12367
                     /gene="eno"
                     /locus_tag="PKM49_04930"
     CDS             11081..12367
                     /gene="eno"
                     /locus_tag="PKM49_04930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNY82190.1"
                     /translation="MYLEISDVFAREVLDSRGNPTIEVEVRLDDGASGIAIVPSGAST
                     GSFEALELRDGEARYMGKGVQRAARNVGEKLAPRLYGMDAYSQALIDMLMIDWDGTSN
                     KSNFGANAILGVSMAIARASADSLDMPLYRYLGGSNAKVLPVPLMNVINGGQHADNNL
                     DIQEFMIVPAGFPTFSEALRAGAEIFFHLKKLLKNEKHATSVGDEGGFAPNLKSNEEA
                     IAIIVKAIETARYKPGKQVFIALDCAASSFFKKQTQSYTIDGASLSSDELLRYYGKLI
                     QNYPILSIEDAFDEEDWDGFAKFQKEYGSRVQNVGDDLYVTNISRLKKGVELGATNSI
                     LIKLNQIGTLTETMDAIQYAKEHRMTSVISHRSGETEDTFIADLAVATNAGQIKTGSL
                     SRSERIAKYNRLLRIEEELDDAAVFNGLDSFYSIKR"
BASE COUNT         3158 a         3103 c         3441 g         2794 t
ORIGIN      
        1 aagcactttt ggccgaaatc acgggagcgg ggagtaccat cggctacctc ctgctgttta
       61 cctccgaaag taagcagaaa cgcttcacac ccacttcata taaggtgatt ggcgttctcg
      121 gcagcatcgc ttcgatcttg ctggcttcaa cgttgaaaaa acaataaaat ccgaatacgt
      181 gaaagaagga gaaacgtgat gttagaaaac ttggacaccg aaaaacggaa ccccaaaacg
      241 atggacctgg accagttctc aatcggcgaa ctgttgcagg cgatgaacga agaagacgcg
      301 acgatccccc tggcgataag ggatgccctg aaagagatct cacaagttgt ggcgacgtgt
      361 ctttcgacga tagagaacgg gggaaagatc tattacgtcg gcgccggaac gagcggacgg
      421 ttggccatca tcgacgccgt cgagctgatc cccactttca acgcgcctgc ggacctgttt
      481 atcccgatta tggccggcgg aaccgaggcc gttttcagag ccctagaagg ttccgaagat
      541 caggaggctt cgggagaaac agagatcgtc agcagggccg tccgaaaaac tgattacatc
      601 atcggtgtca cggcaagcgg gagaacgcca ttcgtcaagg gtgccctcaa aaaggggaag
      661 gaaatcggat gccccaccgc gctgatctgc aatgtgcgaa agcccgaaat cgcgccacta
      721 gcagacaccg tggtgagggt tgtgacgggg ccggaggtct tgactggttc cacaaggctg
      781 aaggctggca ccgcccagaa aatggttttg aatatgatca gcaccgcgac gatggtcaaa
      841 atggggaagg tctttcaaaa ctttatgatc gacgtcgtcg ttctcaacga aaagctccgg
      901 gaacgggcga taaagatcgt caaggagagc accggcgaat cccccgaacg cgcgaaatcc
      961 gaattgatta aggcgaattg gaaagcgaaa caggcgattt tgcagattat caacgatatg
     1021 accccagaac aagcccgcga agccttggaa aagcacggcg gttttttgag gcgggctatg
     1081 aaggtgaaac cgaaatgaag acttttctcg cgatcgacgc cggaggtagc accctacgcg
     1141 cgacttggat gagtggcgac ggaaacctca tccggaaaga aactctcgaa aagaacgcga
     1201 actttctttc cgcgggagaa aaagagatag agaacatcct ccgttttctt aagcacaagg
     1261 tgccgatggt cgacgccctc acgctgtcga tggccggcat cgatgggaaa cctgaatttg
     1321 acaaagtgaa agcgttgtgt gacacgattt ttccaacgac cgcctgtcgt atcttgccag
     1381 acgcggacgg ggcctactac gccaactttg gcatagaacc tggggtcctg gtcatctgtg
     1441 gcaccggctc gatcgtactt ggaaaggacg aagaagggaa gagggttcgt tccggcggtt
     1501 ggggatactt actgggagac gagggcagcg gtttctggtt ggtcaaggaa ctgtttcgta
     1561 agtacctggc atactgcgag tttctcgaag agcgaaaggc gtgtttttcc gtattcgaag
     1621 agcttttccc gggtaatccc agggacgctt tcgtttattt ttaccaaccg gagacacgga
     1681 aacgaacagc ctccttcgcg aatagatttt tgtctttgtc ggatccttac gtgatcgagt
     1741 tgcaccaaag cgggttgaaa tcgctggcca cgcaggtgtt atccgtctta cgccgaatgg
     1801 ggctgaaaca gggacggatt tgcctgatgg gcggggtgtt ttccaacgct caagcggaag
     1861 atttttttag acgctatctt ctaggaaagc tttcggcgtt ggagcaggag cagatttcga
     1921 tcgtcgccgg tgtgaaaaac atctcggacg cgatcgcgca gaagatggtc ggagaaacga
     1981 caagaagttt ggacggagga acggaatgag aatcgctgtg ttgatgaaac aggttccgga
     2041 tacggacgat gtgaagttgg acgaagaaaa gggtacgatg atccgcgagg gcatcggcgc
     2101 cgtcatcaat ccattggatc tgcacgccat cgaagcgggc agacagttcg tggagcagga
     2161 tggcgggaca ttgtcggtct tttctatggg gccccctcaa acggaggagt ccttacggga
     2221 ggccatcgcg atgggcgcgc acaccgcgac gctcctgacc gatcgtaaat tcggcggggc
     2281 ggacacctgg gctaccgcgc gtacgctttg cgagattttg aaactcgagg gcccgtttga
     2341 cctgatctta gccggcgaaa aggcgacgga cggcgaaacc ggacaggtgg gacccgaggt
     2401 cgcggcaatg ctcggaatcc cgttctccac ctacgtgagc gcgatcgaaa agaaaagcga
     2461 cggtgtgatt gtgactagaa ccgtggaaga tggttacgaa acccaattcc ttcccttccc
     2521 gtgccttctg acggtcttga ataacctcaa tcaaccgggt atgcccaccc ttgcggggaa
     2581 gaaacggggc cactcggcga cgctgaaaat cgtgaaagcc gccgatctga gtattcccga
     2641 ggacgagttg ggactcatag gctcgccgac acgcgtgagc aaggtgttct atcccaagat
     2701 ccagcgcaaa tgtgaagttt acacgttatc ggatatacgg acggggatcg aacggatcgt
     2761 ccgcgaactc aagaaccgag cgctcttgtg aaggagataa agatggagaa agagaatcgt
     2821 gtgattccta gaatattggt gatcagcgag gtcaaaaaaa accacatcct accggtaacc
     2881 tttgagctcg ctggcaaggc ggcggaactc gcccgaaaaa tcgaaggggc cgtggatgtt
     2941 ctgctgttct acgatcggct ggacgagccc gtggactccc tgttccaagc cggcgccgac
     3001 cgggcgttgt tggtggagga cagccggttg gggcttttta atcaagagat cgcggtgaag
     3061 atcgggctcg aagtcctacg ccgctacccc gcgcagatcg ttttggccgc cgcgaccacc
     3121 tcgggcagaa cctttttacc cgcgatggcc gcgatcctac acaccgggct caccgccgat
     3181 tgcaccggtc tggacatcga accggaaacc gggttgttgt tgcagacgcg gccggcgatc
     3241 ggaggaaacg ttatggcaac catcaaaacc ccgacccacc gcccccaaat ggcaacggtt
     3301 cgtccgagaa cctttccgaa accggtgccc gtccaacgaa aaggcgccgt cgagcagctg
     3361 gagctgccgg aaagttgctt ttcgagcaag atcgtgccct tgtctcacga aaggatttcg
     3421 gacgactaca cgaatataca ggacctcgac gtcgtggtca ccggtgggaa aggattgaag
     3481 aaacccgaga acttcgcctt gctagagaaa ttggcgaagt tgctgggggg cggtgtgggc
     3541 gcgacgcgac ccaccgtgga agcgaaatgg atcgactacc cccaccaagt gggtctcagc
     3601 ggcaaagtgg tgtccccaaa gttctacttg gcggccgggg tgtcgggcgc ggttcaacat
     3661 ctggccggta tgcagaccgc ggagttcatc gtcgcgatca acaaggatcc tgaagccggc
     3721 atcttcaaga tcgccgatat cggactctgc ggcgatctct ttgagatctt acccgaactc
     3781 atcgcggcat tggagaagga aaggggggag ggacgatgag caacgcctac aatcggctaa
     3841 cggaagaacg aatgggagaa ttgcaggcat tgatggctta tcgccactat acggtcgatc
     3901 cagaacggct gaaggtgtac tctcacgacg aggtcagcgg ggaaaagtac cgagcccttc
     3961 cggagatcgt gttgtaccca gaaacgaccg aggagatcgc ccggattatg aaatgggcga
     4021 acgaaaaccg tatacccgtc acgcccagag gcgccggaac cgggttgtcc ggaggggccg
     4081 tgcccgtgtt ttccggaatc gtcctgtcgc tggagcgtat gaaccggatt ctcgagctcg
     4141 ataaagaaac gctaacgatc acggtggaac cgggagtggt gaccgacgag atacagagag
     4201 cggccaaaag cgcgggcttg ttgtacgccg gtgacccttg cagtagcgat gcctctttca
     4261 tcggaggaaa cgtggctgaa aacgcgggtg gcaacaaagt catcaaatat ggcgcgacgg
     4321 gccggcacgt gatgggtttg gaggtcgtcc ttcctgacgg gcaaatcgca cggttcggcg
     4381 ggaaacttgt gaaagacgtc accgggtacg atttcgtgag cctgatggtc ggctcggaag
     4441 gcacgttggg gattatgacc aagatcacgc tccgattgat ccctctgttg aagtattcgg
     4501 ccgccctatt ggtccctttc gaagacgtcg aaaccgcgat cgccttcgtg ccgaagatga
     4561 tgacacaggc gaagctgctc ccagcgtcga tcgaatttat ggatcaggcg tctctggcgc
     4621 tgaccggacg gtttttgaac agcgagctcc cctacgcgac ggccggggcg catttgatca
     4681 tcgagctgga agaaaacgac aaagggttgc tcttcgacga gtacacgcgt ataggagaga
     4741 tgtgcctgaa atatggcgcg ttggaagtct acgtcgccga caaccgcaac acccgggaaa
     4801 agatatggaa ggcgcgtaaa gcgatcgcgg aggcggtatc cagcttttac cctgagcact
     4861 gtatggagga tatcgtcgtc ccgatcaacc gcatcccaca gattgtggaa aaggttcggg
     4921 acatctcaga acggtaccga ctggaaacga tcaacttcgg gcacgcgggc gacgggaacc
     4981 tccacgtcac ctttttgaac caccgctaca ctaccgagca gtgggaagag actatcgaga
     5041 acgcgttgga tgacctctat agagaaacgg tcctcttggg aggcacccta tcgggtgaac
     5101 acggcatcgg actcaagcgg aaaaaatacc tttccaccgt cctttctccg accgtactgg
     5161 aattaacacg acgtattaaa aatgccttcg atccaaacgg aatcctcaat cccggaaaga
     5221 tcgtcgactg aaaacagaaa ggccggattc ttgtgaattc accggaatta tgttataatg
     5281 aagccgattc tactcttctg ctatcctaaa aaacaaaaac gggggggacc atcatgaaaa
     5341 tggttcggct ttttgttata gtgatccttt ttatgtgctt gatgacttcc tgtacgttct
     5401 ttaaagacct gtttttggac gtgaacaaga taggaaggga tttcgtaacc gcttttgata
     5461 acctcgtaaa gaccttagaa attcctttag gtccgtctac cggggagttt gaaaacgttc
     5521 ggagctttga aggcagagag atcgatccgg tgttaaccgc ttttatgaac acgtacgtgc
     5581 atctggctac gggtgtgacg aagggctcag aagcgtacga agtattggaa ctagtgatcg
     5641 ctattatgac cgtttactca aaaaaggtaa ccttcgtgag cgctgaaaag atcgatatcc
     5701 ccaatatcac gaccggtgcg cgcgtaataa gcccggtatg gtgcggcaat catcccgcct
     5761 acgtttctaa ggggatcctc gtaaaaatga gaatgaatct agacataccg gagtgggatt
     5821 ccgatatgat cgtcgcgtgg cccttgatgc tgatcgataa caaaccctat atgttcaccg
     5881 tgtacaaggc aacagatacc ccaggggaat actacccctt ttatccaatt ccgttctttg
     5941 cactaccatt catacaggtt catagtagca gggaagcagc tgcgacggct ggctaaactg
     6001 tcgacaagaa cttatcgtct attcataatt agaacaaagg aatttatctg ggaggtggga
     6061 taatgaagaa aaaaacactg ctaggcgtac ttgtggtcgt gttggttttt agtttcctgt
     6121 tcgtcggttg cgcgagtgcc acctacaatt ggctctacgg ggtgtggaaa atcgccggtt
     6181 ggggcgtgtt cttgggagat ggccgttcgg taccgtccga tgccgccgcg gaactgaacg
     6241 tgacaaaagt gtggccttcg gaggagttcg aaggtaatta cggattcttg gatggcgcgt
     6301 ccaaaccggt taattatacg attatggatg cgaaagttgt cgaggggaag accgttcgtt
     6361 tttctgtcaa cgataacggg accgttcgga ccttcgtggg cgagctcgtc gtaggcaaaa
     6421 cggctatcgg cacgtgggag tagccgtttc atccgtttaa ggagggaagt gcatgaaaaa
     6481 atctctcgta tttcttttat tagcgctttt ggccatttcg accttttcga tcacgatggg
     6541 catctacgtc gacaatttca aaggggcgat agacgggtgc aaagtcaaag gcgtctatgt
     6601 caacaaagta gagccgaact atccggccta cgggcatatt tgcatcggag acatcatcac
     6661 ggaagcggtc ttagtcggag aaggaaaggt ctggagtcat tgttgtgaac tacctccaac
     6721 cgggagaacc ttcgtcttta accccgacta ttctctgcgg gatcagctgt tcatgctgaa
     6781 ggaagcgcgc gcgaagtatt atttcaatga tatcaaccgc tggcaggata tggcgtacct
     6841 tctgaaccgg gcgcttccgg gagacaccgc gctgttgagg atctaccgga cccaagagag
     6901 aagatggctg tacgtgcccg tttctttagg agccgaaggc cgctatacct ttatgaacac
     6961 gcccacctac caacctcagg tgttcgctca gccagtcgcg gtggttccgg tcgtcgcggt
     7021 tccggtcgtg acctaccaaa ccgtgaccgt gctccccgcg ttcccgctct cgaactgcac
     7081 cgggaccgta ccgcttcaag tggcgcctca accaatcgcc ccagcgccgg ttgtaaccac
     7141 tgtggttcct tcttgctgta gtattattat ctggttgccg taaagaaagg aatacaaaaa
     7201 aagggggcta tcctccccct ttttttgttt gcccgtgcga gatcacagcg ccgctatcgc
     7261 aaaggctcgg ttcccggtat ctcttttaag acaattccgt aaaggagacc tccttcgttg
     7321 agcaacgaat ggttagccgc aatctctatc ttcgtcgatc tatcgctcgt cttggtcctg
     7381 ttccgggcct ttcgaaaaga ggggctttat ctcatcatcg tcgccaatat catcatctgc
     7441 aatatacagg tcttgaaaac ggtgaacctc ttcgggttga ccaccactct cggcaacgtg
     7501 ttgtacggca gtatatacct gacgaccgac atcttgtcag aaacctatgg ccagaaagcg
     7561 gcgaataagg gggtttggtt gggcttcacc gcgctgctct tcttgacgct ctacacacag
     7621 atggccttgt ggttcacacc gcacccggaa gactttgcct cccctcacct cagcgcgctg
     7681 ttttcgctga tgccacgggt cgccttgggc agtctgaccg cctatttcgt ctctcagttc
     7741 ctcgacgtct ggttatttca caaatggaag aacctcaccc gagatcgtca cctgtggtgg
     7801 cgcaacaact tcgcaaccgc cgtcagtcag atagccgaca ctttgatctt ctgctcgatc
     7861 gccttcctcg ggctttaccc gatagagatg tggtggcaaa tcgttctgac gacctatatc
     7921 tacaagttcg tggtggcgct ttgcgatacc ccattcgtat atctcgggcg gaggatgggc
     7981 aaaaaattcg aacaggaggc attgacccat ggataatcgg atttgggagg tcgtccgacg
     8041 tatagaagaa tcacaagcct tggtggtctt gactggcgcg ggggtcagta cggacagcgg
     8101 catctcggat ttcagaagcc cggatggcct ttactcgcgc tgggatcgta atcttgtctt
     8161 caatatcgac tatttccacc aggaccccac ctacttttac cgtttcgcca aggaagagct
     8221 ctaccgcttc gattcgatcg agccgaacgt gactcaccgt ttgttggcgc ttttggaatc
     8281 ggaaggatat ttgaaaatgc tgatcacgca aaacatcgac ggactgcacc agaaagccgg
     8341 gacccgggcg atcatcgaac tgcacggggg tgtctcccaa ggccattgtc agaagtgtgg
     8401 taaagccgtt ccagcggtaa gaatcaagga agcgatcgcc cgcgcgccag tggcgaggtg
     8461 cgacgactgc ggcggcctcg tgaaaccaga tgtcattttt ttcggagaag cgctcgacac
     8521 aagcaacatg gaaaacgcct tctactggag cagaaaagcc gacgtctttc tcgtgctcgg
     8581 gagctccttg caggtgtatc ccgccggcta cctcccggaa gtggccaaac gatctggcgc
     8641 gtacttggcg atcgtcgacc aacaggacac ggcgcaggac gacatcgccg accgcaagct
     8701 ggtgatgccg ttgagggact tttcggaggc gctttacggg gttttctcga gccctgacta
     8761 cccaaaaacc gaccgggggg atcgattcaa ataactgtaa ccgaaataaa cccggaaaat
     8821 tgttcgtcta agttgtaccc ctgaaaaaac tcctccccct tagaggatga agaaacgaaa
     8881 atcgaagaga gaagagattc tctcttcttt tttcgccttg ttcctgtgct ctctgggaac
     8941 gccctgggaa cgcctgggaa cgccgaactt tagttcggca tgtttactgg gaacgccgaa
     9001 cttcagttcg gcatgttttt ggtttttttg gtgttttagc ttttctagga acgcctgaga
     9061 acgccaatct ttaaaggttc tcttaccagt tatatgttta agaactatat tatgggtaaa
     9121 aaaagaattc gaaactgaat gattatgata gaatgcccaa taaaaatgcc aattgggggt
     9181 atataaaatg atcgacaggc ttccagtttt tttcttgcaa gtgctagggt tagtggaacc
     9241 gtggtacatc agcgatgtag aagtaagggg gcttgaaata cacatccgcg tagatttcaa
     9301 acgaggagcg aaatttccct acaaagggga gctgtgtagg gtacacgata cggttatccg
     9361 agaatggcgg catatgaacc tgtttcagta taagacctat ctgcacgcgc gtgtcccgag
     9421 aatctatacg ccggagggtg tcaaacaagt acaagtaccc tgggctcggg aaggaagcgg
     9481 attcacgctt ttcttcgaag cgctgctcct ttctttggct caactaacca gcgtagcgca
     9541 ggtccatcag ttatgcgacg taagtgacaa ccgcatttgg agagtgttag agcactacat
     9601 agagaaagaa gttaaatttc aagatttctc agaagaaccg gttaccacac tctccataga
     9661 tgaagtggcg cgtaaaaaag ggcatgttta cctgaccagc ttcatggact tggaacgagc
     9721 caaggtggtc cacgttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcat
     9841 gtttacctga ccagcttcat ggacttggaa cgagccaagg tggtccacgt ttccgatggg
     9901 aaaggaaaag agtctatttt ctctttcaaa gaacgctatg aggagaaaga aggaaaggtt
     9961 gacgatatcg agaccgtcgt gatggatctc tccccagcct ttatttcagc gatagaagaa
    10021 gcctttccca aagcgacgat cgtctttgac aagttccacg tcgtgaagct tctcaacaac
    10081 gtactggata gtattcgacg cagagaacgc aaagaaaaca tcccaaggtt caacaaaatt
    10141 cgctatctac ttttgaaaga ccccaaaaag ctcaaaaaga gccaaagaga acgactggat
    10201 gaattcctca cccaggctaa cctggataca gtaaaggcgt acaatttgat ccaaatgttc
    10261 aaaagagggt acgaatacca aaagccatcc tgggcaggaa aattctttca tcattggatc
    10321 aagctctgcc aacaatcgaa aatccccgaa atgctgaaag gggcccaagc gattttgact
    10381 catctgaaag gcattatggc ctattttacc taccgggtga ccaacgctgt gatggaaggg
    10441 aacaattcca agctgagaac gatcacaaaa cgctcctatg gattcaagac acttcgatac
    10501 ctgaggttga tgatcttctt ggtcttcgga aaactcaggt tcgatgtacc aaggttgaca
    10561 taactatttt cttacccatt gaacttggaa gagaacctct ttaaattggc atgtcttgaa
    10621 aaaacagggt ttctcacgga gaacacggag ggcgcggaga gagcatagat cgcgacgtaa
    10681 gcgcctcatc tgctcgcgat ggcgcgatac gcttttaaaa ggagaaaggg cgtatcgcgg
    10741 ggccttaaaa accacttacc ccgcgatgac ttgcgcggta atcacggcgt aagcgcttga
    10801 agcttcccag ggcgccccca ggagttcaca gcaaacacaa aaaacctgcc gaactgaagt
    10861 tcggcgttcc taaaaatctt tccttcgtgt tctctgtgtg ctctgtgcga acccatcttt
    10921 tctctttttt aggatatgcc gactaaagtt cggcgcttcg gcacattcct aaagaacggc
    10981 gcccagcgaa gccggcggat aactccgtgt gatggtgtta tgatatacta tgggctgtta
    11041 atcgcacgca aatttcgtac ggaaatggga ggttaacctt atgtatttag aaatcagtga
    11101 tgtgttcgcc agagaggtcc ttgactcgag agggaacccg acaatcgaag tagaggttcg
    11161 gttggacgac ggggcttcgg gaatcgcgat cgtgccgtcc ggcgcttcga cggggtcgtt
    11221 tgaggcgctc gaattacggg atggcgaagc gcgttatatg ggtaaagggg tccaaagggc
    11281 ggcgagaaac gtgggtgaaa aactggcccc gaggctttat ggaatggatg cctacagtca
    11341 agctttaatt gatatgctga tgatcgattg ggatggcacc agcaacaaat ccaattttgg
    11401 cgccaacgcg attctcggtg tttcgatggc catcgcgcgg gcctcggccg attccttgga
    11461 catgcccctg tacaggtatt tgggcggatc gaacgcgaaa gtcctgccag ttcccttgat
    11521 gaacgtcatc aatggcgggc agcacgcgga caataatctg gatatccaag agtttatgat
    11581 cgttcccgcc ggatttccca ctttcagtga agccttgagg gcgggcgccg agatcttctt
    11641 ccatctgaaa aagctcttga aaaatgaaaa gcacgccact tctgtcggag atgaaggcgg
    11701 gttcgccccg aacctcaaat cgaacgaaga ggcgatcgcc atcatcgtca aagccataga
    11761 aaccgcgagg tacaagcctg gcaagcaagt cttcatcgcc ttggattgcg cggccagcag
    11821 cttcttcaaa aaacagacgc agagctacac catcgatggt gcgtcgctgt cttcggacga
    11881 actgctgcgc tactacggga agttgattca aaattacccg attctgtcga tcgaagacgc
    11941 cttcgacgaa gaagactggg acggcttcgc caaattccaa aaagagtacg gaagtcgggt
    12001 tcagaacgtg ggagacgacc tctacgtcac caatatcagc cggttgaaga aaggggtcga
    12061 actgggcgcg accaactcta ttttgatcaa actgaaccaa atcggcacgt tgacggaaac
    12121 gatggatgcc atccaatatg ccaaagaaca tcggatgacg agcgtgatct cccaccgatc
    12181 cggggaaacc gaagacacct tcatcgcgga tttggcggtc gccacaaacg cgggacagat
    12241 taaaaccggc tctctttccc gtagcgaacg catcgccaaa tacaaccggt tgctccgcat
    12301 cgaagaagaa ctcgatgacg cagccgtttt caacggcctg gacagctttt attccattaa
    12361 gcgctgaaat aacccaagcc cggaataaac ccgttgcccg cccccgtcga acaccggaaa
    12421 accctcagaa ggcgagaact ccggtatttt tttcgtgcgt gatggctatg gcttgagaag
    12481 gagtatttgt gcggaccttt tggttgtttt tctgtaacga atggaagtat gtcttgcgga
    12541 acccgttgtt cttcgcgatc ctgatcttga ctccgatctt gttgctcacg gtttcg
//
LOCUS       DAOPXN010000061        12554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5106_length_12554_cov_4.413873, whole genome shotgun sequence.
ACCESSION   DAOPXN010000061 DAOPXN010000000
VERSION     DAOPXN010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12554
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5106_length_12554_cov_4.413873"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3667
                     /locus_tag="PKM49_04935"
     CDS             <1..3667
                     /locus_tag="PKM49_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82191.1"
                     /translation="IRWIQVLPLLPFQPLNTLHEKSLLNEVIYHGSRMSESMRWPHPS
                     HAGVIDLLESPESEEMGRVLTKVPGAQYLPEELRFLPASVETEKVQSLMSWATRTVPF
                     ANYSDGPRVMMGGKNLKQAVKVVSGERPIIGTGVEEELRMDWGVNAFVGYALYHGLNF
                     EDGIVASEGFAKRMAIEREEELREDVEFPCPAVFQMQYDRRDNRVVLSDSDAGSRSAI
                     SVELVFKRKGHILTRNGEFGSITVYFVDKDRSLPAKKLLSKVLIYKERYPAELLLDLD
                     NEDHRERLIRFVQRKYPFGKAKGTKEEKAGVVLNTSIVELRVPIFVRKPLEVGDKITG
                     RHGNKGTISKVLPDSSMPYVLKNGQRKPLDLILSPFGIITRMNLGQLLETHLSLVSER
                     TQKSFTTVDPADCLKHMKESGFVTDLDAEFGKADLLLDGYRVSATVGYQYFVRLNHCV
                     RDKLHVISKTDRINLNTFQPYKGRSNDGGQRMGEMEFWTLFNHGATELIDLFAHTNLS
                     DWKDKEKYIARLNEILQYYHRYTLSTKAAQGKVILTKARAESISLKNLLLKEMKNPED
                     ASLVYRLIKRDIRKQCVKRLLASKEGYIRKCMLGRRVHYSGRATITPATDITIDQVKL
                     PLEFALEWFPHFKISARDRDRVFNGDRSKRAEICKRVNESLPEGLFVLLNRQPSLHRH
                     SMASFKPVFWEEYAIGLPIMVCEGFGADFDGDTMAAFYPITQEKNVRMQEELEGMRPS
                     RHPFRSGNRDFAYSLSQDLLFGYWRKWGSAKIKSHVKDILNSSKPDEIPDRLLSFQTE
                     CLDEATRQNVSLSFYEVVHQKGDLLPIMESKARGKEAHFEKMKRREGADSFGSGHTVS
                     SYFGLGEDREPLPDFDERIVVQTRRSMMDKKFHVGEGGYFTRKLVELLYPVRVTESDC
                     GAQEGLRFPRAYLEELIDKIVLEGSANLSQNALEVRKERASFLFRKLFLNRYVRDKHG
                     TWALVDEEALEHLFRSSEDIEVRSPVKCATKNGMLCSRCLGLDPSKIREGFAIDDFVG
                     VMAGHTIGERGTQLSMKTFQTGDYGFRMQRVSSFFFNLEGACDKGSLGYLEYFRRICL
                     ASVQRIMSADTGQSVDMLATQESASDVGGSLLQTIDVISLHLEILYREMSRLNLQTEA
                     QMLDCLTDWRKRGFFTALSFENPRKRKGGKEKSDTEKSEAIIASEFGAENEDSIEISP
                     KTSYAFCLKWEDNYGSV"
     gene            3654..4541
                     /locus_tag="PKM49_04940"
     CDS             3654..4541
                     /locus_tag="PKM49_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82192.1"
                     /translation="MEAFKRQTRESFKNKSLLKSASRKKRVPNLDIFGEENPEDEIPT
                     DESGLEREEETVEVNDDEVAPTLAPEKTSLFESDLEVPFAEARGMRVVLLLAKQESGE
                     MTVFLNGERSYEDIEMNHTGAYYSNVKRILQRFCSGFFDGERPPEPEDFFARFKSTWF
                     FGEKNTSKTNRRYNTDYAIEFWNGERVALNLLTPNGLMPEKTEAATLFRIALGADPAI
                     LKNRFRDLCCDYASQGNLDGTLEVLSYFKGLTYALGDMGIDLSDRKEDFLFSALKGQP
                     FFISLVQANGRNDRACGPK"
     gene            4516..5931
                     /locus_tag="PKM49_04945"
     CDS             4516..5931
                     /locus_tag="PKM49_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY82193.1"
                     /translation="MIVPAAQSRVSLLVIEDKKAKEASEVVKEIITTTLKSKNLDAYV
                     EVRFEADYNLQGAATDILVINTPQFKPEDVVSSGKNPYIFLLFTGSLSVETARAAQKS
                     GRFELFPTDRFYGERIEYSEIARFAESLKRVVRELAGESVLEYHFSKSLDWKIRFDHK
                     EKGAVSSFIADPVFQAVLSQAEKALGYLRGFQTELIEFRQKTKRSLSELGAYENDVSG
                     ADLNAVKRGFSEINAHAAKMKTKKIHGILLTGETGSGKTRVAEWIASRFLDKDFASHY
                     GKIPLVNIAPNLIDSELFGTFPGAFTDARYKTGKLLSCAGGVVFLDEIGEIPATVQAK
                     LLTFLDDRKALVEGYNDPQGVVIPLLLIAATNRNVREEIKKGDFRNDLYHRFLFKLHI
                     PPIRDRKNDFRYLLSYVLQSQNANIPDSSVESISLKAIETLEKHDYPGNFRELEDIVR
                     RALLNADFARRKCILAKDIEY"
     gene            complement(6054..7178)
                     /locus_tag="PKM49_04950"
     CDS             complement(6054..7178)
                     /locus_tag="PKM49_04950"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HNY82194.1"
                     /translation="MKKTCPNPAFLGFLIATAILVAIPGLSYVKQRAPETIIPGPGVT
                     RRGSLSDYLPALKGTSADTPVFYLEGKEPGGTVLLVGGAHPNEPAGFIAPLIVVERGV
                     AQKGRVIVVPYANGSAISHTEPLEGSPASFSLKNKAGETRVFRVGSRLSNPLDQWPDP
                     ALYVNEKGQVLSGSEMRNLNRAFPGKADGSITEQIAYAIMEIIRREKADISIDFHEAS
                     PEYTTVNAIVAHEKSLELAAEVAMYLEFDDIPITLEQSPKNLNGLSHREWGDYSDTLP
                     FLFEAANPFMGRLRGKTDETLIIEGKDPYYLAAAKAKRLNVPYDEKGLPLSYRVARHI
                     TAFRYVLDTYNTYYEEKAIIVTDLPEYEEMAYRNLGDFLN"
     gene            complement(7194..7340)
                     /locus_tag="PKM49_04955"
     CDS             complement(7194..7340)
                     /locus_tag="PKM49_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82195.1"
                     /translation="MMKWLYLGILCFLIVLLIRYTFTSKKKWEWLMAAFVLAPWILRL
                     LQLK"
     gene            complement(7337..8656)
                     /locus_tag="PKM49_04960"
     CDS             complement(7337..8656)
                     /locus_tag="PKM49_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNY82196.1"
                     /translation="MQTEFIILLIMLGSFILFAALRFPISISLIVAAILGALCSGFGF
                     PLARLVEGAFGYLDAIMIIATAMIFMRSIERSGLLKTLANAIARVLHNRPYLLLAFMT
                     LFIMFAGMITGSSTASVLTTGAIAYPVLTQLGLDKKRAGAIIAMSAIFGMIAPPVNIP
                     VMIIGQGVDMPYMGFTYPLLWITIPLAFLTSFLFGGKYIHRIPKESIEKGTGGFLVYA
                     PFILLIVLMLLEELRVLDLGMPLVFLLCALCATVTGKRFDFFKSSVEAIGDSLNILSI
                     LVGVGMFIQVMTMTGVRGFVVGSFVSIPQSVLFLTMAVGIPLFGAVSSFGSASVLGVP
                     FLLSFLSGDVIVVTAALSMLAGLGDMVPPTALAGIFAAQVVGEKSYFKIFARSLISVA
                     ITIVACVFVVLYSKHVAALPPWLFWVVSGGFLGVLSLIKGKEAPKVV"
     gene            complement(8846..9886)
                     /locus_tag="PKM49_04965"
     CDS             complement(8846..9886)
                     /locus_tag="PKM49_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HNY82197.1"
                     /translation="MRKHFLICLCVLLAFSFSAFATAYLPFTYDIRSGVGVTEQRRLS
                     DYLDALRYTPGDTPVFVLDSGKAGANVLVLGGTHANEISGIMAAILLVERASVETGRL
                     FVIPFTNGSAITWRDNKNAVIPPWFEFTTPSGVRRLPYGSRLTNPEHEILPDPERFYL
                     ENGIPLDGSEARNLNRVHPGLPDGTLTQRISAAITSLIFEEEIDLVDDFHESGTKSRL
                     AYMLVCNPKNLDLGAFAIIDLQMQGIDLKLEESSAEFLGLSHREFGDKTPAFAFLFET
                     PNPGQDSDLKQPPDVVNDLIYPLYHRVSLQVAVLQTLCQYLPDFTGKTIEIVNIPPFE
                     VLNSERLYDYYQ"
     gene            complement(9903..10457)
                     /locus_tag="PKM49_04970"
     CDS             complement(9903..10457)
                     /locus_tag="PKM49_04970"
                     /inference="COORDINATES: protein motif:HMM:NF040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HNY82198.1"
                     /translation="MKKRVFLLAFTLVFAILVIANGPAVPKLAEPVMITTAGQSAGAA
                     MMKVLFTKSNIKDFVFEKLVTADEIEGYETLVIVAGASSKGLGAAGIDFDGETQRVTA
                     LIEAAKKQGMKVVVAQIEGAARRGTSSDQLFSLFVPMSDWVIIVRDADSDGFFTNLCE
                     EHGIPLTIVEKSIEVSAQLNAVFE"
     gene            complement(10585..12318)
                     /gene="ggt"
                     /locus_tag="PKM49_04975"
     CDS             complement(10585..12318)
                     /gene="ggt"
                     /locus_tag="PKM49_04975"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HNY82199.1"
                     /translation="MKRFASILLIVSVLSLSVFGQIVTGQTAIAMNGMVASASPYAAE
                     VGARVLEMGGNAVDAAVAMGFALGVVEPYASGPGGEGFLVVRLADGTTKTIDFKSTAP
                     SDLSLYTKAPSNYSTGPLSVCVPGVVDGLLKALEEWGTMSREELISPAVKLAEEGFIV
                     SGDFAGNLSDAYEKILNAPGDIANVYLNDLMLYTAGDIFKNPDQAAILREIIAGGSEA
                     FYNGTFADKLDAYFRANGGAIRKIDLANYKAKIKDAVSGTYRGYNIYSVPPSVAGAQL
                     IEGLNILEYFNLSAFEYDDPMVISLIADALYLASIDRRNYIEDSDTVPVKGLISKEFA
                     KSRLPLLTLGKGLKRITNTNERNAAYAADPWPYELGESYVDVELSGVAALAEPAYESP
                     STTHYSVVDRWGNAVSVTQTLSSFFGSCVVVPGTGILLNNEWANFSSSGINKFVPGKN
                     PRTIICPTIITDPNDELFMVVGSPGASRILSMITQIIVNVIDFKMPVTAALKAPKTAT
                     YDTAAELFIEGGYPENTLKALESQYGYKLIQYPGIDLFFGGANIVYRYPDGLLLGIGS
                     YRRSGGAAGID"
BASE COUNT         3122 a         3132 c         3453 g         2847 t
ORIGIN      
        1 gatccgctgg atacaggttc ttccgcttct gccctttcaa cccctcaaca ccctgcacga
       61 gaagtcctta ctcaacgaag tgatctacca cggaagccgg atgtccgaat cgatgcgctg
      121 gcctcatcct tcacacgccg gtgtgatcga tctcctcgag agtcccgaat cagaggagat
      181 ggggcgcgtt ctgacgaaag tacctggagc tcagtacctg cccgaggaat tgcggtttct
      241 ccctgcttcg gttgaaacag aaaaggtaca aagcctgatg agctgggcga cgcgaacagt
      301 tcctttcgct aattattccg acggaccgcg cgtgatgatg ggtgggaaaa acttgaagca
      361 agcggttaag gtcgtgagcg gggagcgccc catcatcgga accggggtgg aagaggaatt
      421 acggatggat tggggggtga acgccttcgt tgggtacgct ctctatcacg gtttgaactt
      481 cgaggacggc atcgtcgcca gcgaaggctt cgcgaaaagg atggccatcg aaagggaaga
      541 ggagttgcgt gaggacgtcg agttcccttg tccagcggtc tttcagatgc agtatgacag
      601 gcgagacaat cgtgtcgttt tgtcagattc cgatgctggt tctcggagcg cgataagcgt
      661 ggagttggtt tttaaacgga aagggcatat tttaacacga aacggcgagt tcggatcgat
      721 caccgtctat tttgtggata aagaccgctc gcttcccgcg aagaagcttc tttcgaaagt
      781 cctgatttat aaagagaggt acccggcgga gctgctcttg gatttggata atgaggatca
      841 tcgggagcgc ttgattcgct tcgtacagag aaagtacccc tttgggaagg cgaaagggac
      901 gaaggaagaa aaagccggtg ttgtactcaa cacgagtatt gttgagctgc gtgtgccgat
      961 cttcgttcga aagccgctcg aagtggggga taaaatcacc ggaaggcacg gcaataaggg
     1021 cacgatttcc aaggtactgc cggattcttc gatgccctat gtgttgaaaa acgggcaacg
     1081 aaaaccgctc gatctcattt taagtccttt cgggataatc acccgaatga accttgggca
     1141 gctactggaa acccacctgt ctttggtgtc cgagcggaca cagaagagtt ttacgacggt
     1201 ggacccggca gattgtctca aacatatgaa agaatccggg tttgtgaccg atctggacgc
     1261 tgagttcggc aaagcggacc tgctccttga tgggtatcgt gtttccgcca ccgtgggtta
     1321 tcagtatttc gtcagactca atcattgcgt gagagacaag ttgcacgtga tctcgaaaac
     1381 agatcgcatc aacttgaata ccttccaacc gtataagggg cgctcgaacg acggcgggca
     1441 acgtatggga gaaatggagt tttggaccct cttcaatcac ggcgctacag aactcatcga
     1501 ccttttcgca catacaaatc tgtcggattg gaaggataaa gaaaagtata ttgcccggct
     1561 caacgaaatc cttcagtatt accaccggta tacgctctcg acaaaagcgg ctcaaggaaa
     1621 agtgatcttg acgaaggcac gcgcggagtc tatttctttg aaaaacctgc ttctcaaaga
     1681 gatgaaaaac cccgaagacg cctcactcgt gtatcggctc attaaaaggg acatccgcaa
     1741 acaatgcgta aaaagattgc tcgcttcgaa agaggggtat atacgcaaat gtatgctcgg
     1801 gcgaagggtt cattattccg ggagggcgac gatcactccg gcgaccgata tcacgatcga
     1861 tcaggtcaaa ctaccgttgg aattcgcgtt ggaatggttt ccgcatttca aaatatcagc
     1921 gcgagataga gacagggttt ttaacggaga ccggagcaag agggcggaga tttgcaagcg
     1981 ggtaaatgaa agccttcccg agggtctttt cgtgcttctg aatcgacaac cctcgctgca
     2041 tcggcacagt atggcttcct tcaaacccgt tttttgggag gagtacgcga ttggcctgcc
     2101 gattatggtt tgcgaaggct ttggagccga cttcgacggg gacacgatgg cggcgtttta
     2161 tccaatcacc caagaaaaaa acgtgaggat gcaggaggag ttggaaggta tgcggccttc
     2221 acgtcatccg tttcgttctg ggaataggga tttcgcgtac tcgctttcgc aggacctcct
     2281 tttcggatac tggcgaaagt ggggcagcgc gaaaatcaaa agccacgtga aagatatcct
     2341 taactcatcc aagccggacg aaatacccga ccgcttactg tcctttcaaa cggaatgttt
     2401 ggatgaggcg acgcgccaga acgtcagcct atccttctat gaagttgtcc atcaaaaagg
     2461 cgatctcctc ccgataatgg aatctaaagc gcgcgggaaa gaggcccatt tcgaaaaaat
     2521 gaagcggcga gaaggagcgg actcctttgg ctccggccat acggtgtcga gttattttgg
     2581 tctaggggag gaccgagaac ccctacccga ttttgacgag cgcatcgttg ttcaaacccg
     2641 ccgttcgatg atggataaaa aattccacgt gggagaaggc ggctacttca cccggaaact
     2701 cgtcgaactg ctgtatccgg tacgtgtgac ggagtcggat tgcggcgcgc aagaggggct
     2761 gcgctttccc agagcctatc tggaagagct gatcgataag atcgttctgg aaggctcggc
     2821 caaccttagt caaaacgcgc tcgaggtccg taaagagaga gcgtcttttt tattcagaaa
     2881 gttgttcctt aaccggtatg ttcgcgataa acacggaaca tgggcgctgg tcgacgagga
     2941 ggccctcgaa catctatttc gatcgtcgga ggacatcgaa gtgagaagcc ccgtcaaatg
     3001 cgccacaaaa aacggtatgc tctgttctcg ttgtttaggt ctggaccctt cgaaaattag
     3061 agaagggttc gcgattgacg attttgtcgg ggtgatggcc ggccacacga taggagaacg
     3121 cggtacgcag ctctcgatga aaaccttcca gactggcgat tacgggttca gaatgcaacg
     3181 ggtatcctcc tttttcttca atttagaagg cgcctgtgat aaaggtagtt tgggatattt
     3241 agagtacttt agaaggattt gcttggcttc cgttcagcgg attatgtctg cggatacggg
     3301 acaaagcgtt gatatgctcg ccacgcaaga gtctgcgtca gacgtggggg gatcgcttct
     3361 tcaaacgatc gacgtcatct ctctacattt ggagatcctc tatcgagaga tgtcacggct
     3421 taaccttcaa acggaagcgc aaatgctcga ttgtctcaca gactggagaa agagaggctt
     3481 ttttacggcg ctgagttttg aaaaccccag aaagaggaaa ggtggaaaag aaaagtcgga
     3541 tacggaaaag agcgaggcga ttatcgcgtc ggaattcgga gctgaaaacg aagactctat
     3601 agaaatctcg ccgaaaacct catatgcctt ttgtctgaag tgggaggaca actatggaag
     3661 cgtttaagcg gcaaacgcgg gaatcattca aaaacaagtc ccttttaaaa agcgcgtcca
     3721 ggaaaaagcg cgttccgaac ctggatattt ttggcgaaga gaacccggag gatgaaattc
     3781 cgactgacga gagcggtttg gaaagggagg aagaaaccgt agaggtcaat gacgacgagg
     3841 tagccccaac ccttgcgccg gagaagacct cgttgtttga gagcgatctc gaggttccgt
     3901 ttgccgaagc ccgcgggatg cgcgtcgtct tgctcctcgc gaaacaggag tccggcgaga
     3961 tgacagtgtt cctaaacgga gagcgaagct acgaagatat cgagatgaat cacacagggg
     4021 cctattattc aaacgtcaaa cgtatattgc aacgcttctg cagcgggttc ttcgacggcg
     4081 aaagaccgcc ggaacccgaa gactttttcg ccaggttcaa atccacgtgg ttctttggcg
     4141 agaaaaatac ttctaagacc aatcgccgat acaataccga ctacgcgata gagttctgga
     4201 acggtgaaag ggtggcgcta aacctattga ctccgaacgg actaatgccg gaaaaaacgg
     4261 aagcggccac gctttttcgt atcgcgctcg gcgcggatcc ggccatattg aaaaatcgat
     4321 ttcgtgatct ttgctgtgac tatgcgagtc agggaaacct tgacggcact ttggaggtat
     4381 tatcgtattt caagggactg acctacgcgc tcggtgatat ggggatcgat ctcagcgatc
     4441 gaaaagaaga tttcctcttt tcggcgttga aagggcagcc gttttttata agtttggtac
     4501 aagcgaatgg gaggaatgat cgtgcctgcg gcccaaagta gagtgagcct cctcgtaatc
     4561 gaggataaaa aagccaaaga ggctagtgag gtcgtaaaag agatcatcac aacgactttg
     4621 aagagcaaaa accttgacgc ttacgtcgag gtgaggtttg aggcggacta caatctgcag
     4681 ggagcagcca cagacatcct ggtcatcaat acccctcagt ttaagcccga agacgtagtt
     4741 tcctcgggta agaaccccta tatctttctt ttgttcacgg gcagcctttc ggtcgaaacc
     4801 gcgcgcgcgg cgcaaaaaag cggacgattc gagctgtttc cgaccgatag gttttatggg
     4861 gaaaggatag agtattcaga aatcgcgagg tttgcggaat cgctgaaacg cgtagtccga
     4921 gagctcgcgg gagaaagcgt tttagaatac catttttcta agagcctcga ttggaagatc
     4981 cggtttgatc ataaagagaa aggagccgtt tcatccttca tcgcggatcc ggtgtttcaa
     5041 gcggtacttt ctcaagcaga aaaagcgctc ggttacttgc gagggtttca aacagaatta
     5101 atagaatttc gacaaaaaac gaagcgctca cttagcgaac tcggcgcgta tgagaacgac
     5161 gtttcgggcg ccgatctcaa cgcggtaaaa cgcggattca gtgagattaa cgcgcacgcc
     5221 gccaaaatga aaacaaaaaa gattcacggt atactcctaa caggagaaac cggtagcggg
     5281 aagacccggg ttgcggaatg gatagcgtct cggttcttgg acaaggactt tgcgagccac
     5341 tatggaaaaa tcccactcgt gaatatcgcg ccgaacttga tcgattcgga gctgttcggg
     5401 acttttccgg gcgctttcac ggatgcgagg tacaagacgg gaaagctgct gtcttgcgcc
     5461 ggaggcgtcg tttttctcga tgaaataggg gaaataccag caacggtaca agcgaaattg
     5521 ttgactttct tggacgatcg caaagcgctg gtggaaggtt acaacgatcc acagggggtc
     5581 gttattccgc tgttgctcat cgctgcgacg aatcgtaacg ttcgagagga aataaaaaaa
     5641 ggcgatttca ggaacgatct ctatcatcgt ttcctcttca aactacacat tccaccgatt
     5701 agagatagaa agaacgactt tcggtatcta ttgagctacg tcctccaatc acaaaacgcg
     5761 aacattcccg attcttcagt ggaatccatc agcttaaagg cgatcgaaac cttggaaaag
     5821 cacgattatc cgggcaattt ccgcgaattg gaggatatcg taaggcgtgc gcttctaaac
     5881 gccgacttcg cgcggaggaa atgtattttg gcgaaggata tcgaatactg agcgaaaacg
     5941 gcctctgacg acgctttttt gcgtaagcga cgggcgcgcg cagattgaga ttttcaccgg
     6001 ggcgtgaaag aagaaaaagg gggattcacg cggcgctccc acggcggtcc cagttagttc
     6061 aagaagtccc cgaggttccg ataggccatt tcttcgtatt cgggcaagtc ggtgacgatg
     6121 atcgcctttt cctcataata tgtattgtat gtgtccagca cgtatcggaa ggcggtgatg
     6181 tgtcgggcga cccggtagga gagcggtaaa cctttttcat cgtacgggac gttgagtcgc
     6241 ttcgctttgg cggcggccag gtaataaggg tctttgcctt ctatgatcag ggtttcatcc
     6301 gtctttccgc gtaaccggcc catgaagggg ttagcggcct cgaataggaa cggtagggtg
     6361 tccgagtagt ccccccactc gcggtggctc aatccgttta ggttctttgg cgattgttcg
     6421 agcgtgatcg ggatgtcatc gaattccaag tacatagcca cttcagccgc tagctccagg
     6481 cttttttcgt gggcgacgat cgcgttcacg gtcgtgtact ccggtgatgc ctcatgaaaa
     6541 tcgatggaga tatcggcctt ttccctccgg atgatctcca taatcgcata ggcgatctgt
     6601 tcggtgatcg atccatctgc ctttccgggg aaggcgcggt tgaggtttct catctccgac
     6661 ccactcaaca cttgcccctt ttcattgaca tatagagccg gatccggcca ctgatcgagg
     6721 ggattggaaa gtcgagaccc cacgcgaaag acacgggttt ctccggcttt gttcttaagg
     6781 gaaaaggagg cgggggagcc ttccaagggt tcggtgtggc tgatcgcgct tccgttggcg
     6841 taagggacca cgattactct ccctttttgc gccacccctc tttctaccac gattaaaggc
     6901 gcgatgaatc ccgcgggttc gttcggatgc gccccgccga cgagtaaaac cgttccgccg
     6961 ggttcttttc cttcgaggta aaataccggt gtgtccgcag acgttccttt caacgccggt
     7021 aagtaatcac tcagggaacc tcgtcgggtg actcccggcc ccgggatgat ggtctcgggc
     7081 gcccgctgtt tgacatagga taggccggga atcgccacca ggatagcggt cgcgatcaga
     7141 aaccccagga atgcggggtt tgggcaagtc tttttcatcg cattcacctc tctctatttg
     7201 agttgcagta ggcgcaatat ccacggggct aacacgaaag cggccatcaa ccactcccac
     7261 ttcttcttcg aagtgaaggt gtagcgtatc agtaggacta tgaggaaaca caggataccc
     7321 aaataaagcc atttcatcat acgaccttcg gcgcctcctt tccttttatg agcgaaagaa
     7381 ccccaaggaa tccaccggat accacccaga agagccaagg gggtaacgcc gcgacgtgtt
     7441 tcgagtagag aacgacgaag acgcatgcga cgatcgtgat ggccacggag atcaaagagc
     7501 gggcgaagat tttaaagtag ctcttttccc ccaccacttg ggcggcgaat attcccgcca
     7561 gagcagtggg cgggaccata tctcccaaac cggcgagcat cgagagcgcg gctgtgacca
     7621 cgatcacatc accgctgagg aaggagagga gaaaaggcac tcctagaacg gaagcggaac
     7681 cgaatgaaga gaccgcgcca aatagcggga taccgaccgc catcgtgagg aagagtacgg
     7741 attgcgggat gctgacgaaa ctgccgacca cgaagcctct cacgcccgtc atcgtcatca
     7801 cctggatgaa catccccact ccgacgagga tcgataggat gtttaaagaa tctccaatcg
     7861 cttcgaccga ggacttgaaa aagtcgaatc gcttgcccgt taccgtggcg catagcgcgc
     7921 agagcaagaa gaccaagggc attccgaggt caagcacgcg cagttcttcg aggagcatca
     7981 agacgatcag caagatgaag ggggcgtaga cgaggaaacc gccggtaccc ttctcgatcg
     8041 attccttcgg gattcggtga atatacttgc cgccgaaaag gaaactggtt aggaaggcga
     8101 ggggaatggt gatccacaag agggggtagg tgaagcccat atagggcata tcgaccccct
     8161 ggccgatgat cattaccgga atgttcaccg gtggggcgat catcccaaaa atcgcggaca
     8221 tcgcgatgat ggcgcccgct ctcttcttat ccagaccgag ttgcgtcagc acagggtagg
     8281 caatggcgcc ggtcgtcaat acggaggcgg tggaggagcc ggtgatcata ccggcgaaca
     8341 tgatgaacag agtcataaag gccaacagga ggtacggcct gttgtgaagc acgcgggcta
     8401 tcgcattcgc gagcgttttc agcaagcctg agcgttcgat gctgcgcatg aagatcatcg
     8461 ccgtcgcaat aatcatgatc gcgtccaaat atccgaaagc cccttcaacc aacctggcca
     8521 aaggaaaacc aaaacccgag cagagcgcgc cgagaatggc cgcgacgatg agcgaaatcg
     8581 agatcggaaa acgcaaggcc gcgaaaagga tgaaagaacc gagcataatc agcaggatga
     8641 tgaattctgt ctgcaaatag aacacctctt atcgatggaa tgaatccccc cgttttggat
     8701 tctgagcccg aaaggacagt accgggcgcc ctgcgcggtt cgccggagaa tcggacatag
     8761 agagccggaa agcccggcgc atctaacaac gacgcaggcc gtctttccgg ttttaagaat
     8821 cagcctgtcc gtaaaatctc ggcgatcact gatagtagtc gtacaaccgc tccgaattca
     8881 acacctcaaa cggtggtatg ttgacgattt ctatcgtttt tcccgtgaag tccggcaagt
     8941 actgacaaag ggtttgcaga acggccactt gtaaagaaac ccgatggtag aggggataga
     9001 taaggtcatt cacgacatcg ggaggttgtt tgagatcgct gtcctgtccc ggattgggcg
     9061 tttcaaagag aaaggcgaac gccggcgttt tgtccccgaa ctctctgtgg ctcaaaccca
     9121 agaattccgc gctggactct tcgagtttca aatcgatccc ttgcatctgc aaatcgatga
     9181 tggcaaacgc gcccagatcc aggtttttcg gattgcacac gagcatatac gcgagtctcg
     9241 atttcgtgcc cgattcatgg aaatcgtcga caaggtcgat ctcttcttcg aagatgagag
     9301 aggtaatcgc ggcgctgatt ctttgcgtga gcgtaccgtc gggaagtccc gggtgaacgc
     9361 ggttcaggtt tctggcttct gaaccgtcca gtgggatgcc gttttcgaga taaaaacgct
     9421 ccggatcagg taggatttcg tgctccggat tggtcaaccg cgaaccatag ggaagccgtc
     9481 gtacgccgga cggagtcgtg aactcaaacc agggaggaat aacggcgttt ttattgtcgc
     9541 gccaggtgat cgcgctgccg ttcgtgaaag ggatcacgaa cagtcttccc gtttcgaccg
     9601 acgcccgctc gacgagaaga attgcggcca taataccgga aatctcgttt gcgtgcgtcc
     9661 cacccaaaac gaggacgttc gcgcctgcct tcccgctatc taaaacgaaa accggtgtgt
     9721 ctcccggcgt gtacctcaac gcatccaagt aatcgctcaa acggcgctgt tcggtaaccc
     9781 ctacgcctga gcggatatcg taggtgaagg gcagataggc tgtggcaaag gccgagaacg
     9841 agaacgctag caacacgcag agacagatta aaaagtgttt tctcatcagc taggacccgc
     9901 ccttattcga aaacggcatt cagctgagcg gagacctcaa tcgacttttc gacgatggtt
     9961 agagggatgc cgtgttcttc gcagagattg gtaaagaacc cgtcgctatc cgcatctctg
    10021 acgatgatta cccagtccga cataggaacg aacagagaga agagctgatc gctgctggta
    10081 cctcgccggg cagccccttc gatttgcgcg acgaccacct tcatcccttg ttttttcgcg
    10141 gcttctatca gagcggtgac cctttgcgtc tcgccatcga agtcgattcc ggccgccccg
    10201 agtcctttgg agctcgcgcc ggcgacgatg acgagcgttt cgtatccttc gatttcgtct
    10261 gcggtcacca atttctcaaa aacgaagtct tttatattcg atttcgtgaa caataccttc
    10321 atcatcgcgg cgccggcgct ttgacccgcg gtcgtaatca tcacaggctc ggcgagtttg
    10381 ggaacggccg gcccgttggc tatcacgagg atagcgaaaa ccaacgtgaa agccagtaag
    10441 aaaacccttt tcttcatgga agtaccctcc tcattatgcg tttgattgcg ctattcgaat
    10501 ctaaacctaa gaaagggagg cggaaacccc cgatgttgct tccgcctacc cttgacgtga
    10561 ataacttatc gatagaaaac tgtcttaatc gattccggca gcgcctccgg agcgtctgta
    10621 ggacccgatg cccaacaata ggccatcggg gtagcggtag acgatgttcg cccctccgaa
    10681 gaagagatcg ataccgggat actgaatcaa cttgtacccg tattggctct ccaacgcttt
    10741 tagagtattc tcagggtatc cgccttctat gaacagctcc gcagccgtgt cgtaggtagc
    10801 tgtcttcggc gctttcaaag cggctgtgac cggcattttg aagtcgatga cgttgacgat
    10861 gatctgggtg atcatcgaga ggattctcga agctccgggg cttcccacga ccataaacag
    10921 ttcatcgttc gggtcggtga tgatcgtcgg gcagataatc gtcctggggt ttttccccgg
    10981 tacgaatttg ttgatcccgc tggaagagaa gttcgcccat tcgttgttca gcaggatacc
    11041 ggtgcctgga acgacgacgc aggagccgaa gaacgagctc agggtttgcg tcacgctgac
    11101 cgcgttgccc catcggtcca caacactgta atgggtcgtg gaaggagatt cgtaggcggg
    11161 ttccgccaaa gccgcgacgc cggatagctc aacgtccacg taggactctc ccaattcgta
    11221 aggccaaggg tcagcagcat aagcggcgtt tctttcgttt gtgttcgtaa tgcgcttcaa
    11281 tccttttccg agcgtcaaca gcggcagcct ggatttggcg aactctttcg agatcaatcc
    11341 tttcaccgga acggtgtcgc tgtcttcgat gtagttccgc cggtcgatcg atgccaggta
    11401 caacgcatcg gcgatcagag agatcaccat ggggtcatcg tattcgaacg cggacaggtt
    11461 gaagtattcg aggatattga gcccttcgat cagctgggcg cccgccacgc tcgggggcac
    11521 cgagtagatg ttatacccgc gataggtacc cgatacggcg tcctttattt tcgctttgta
    11581 gttggccaaa tcgatcttcc tgatcgctcc gccgttcgct ctgaaatagg cgtcgagttt
    11641 gtcggcgaaa gtgccgttgt agaatgcttc actgccgccc gcgatgatct ctcgaaggat
    11701 tgcggcctga tccgggtttt tgaagatatc cccagcggta tagagcatca ggtcgttaag
    11761 gtatacgttc gcgatgtctc ctggcgcgtt cagtattttt tcgtaagcgt cggataagtt
    11821 cccggcgaaa tcgcctgata cgataaaccc ttcttcggcc aatttaacag ccggagagat
    11881 gagctcttct cgagacatcg tcccccattc ctcaagggct ttcagcagcc catccacaac
    11941 gccgggaacg cagactgaca aaggaccggt ggaataattg ctcggagctt tcgtatacag
    12001 agacagatca gaaggcgccg tggatttaaa gtcgatggtt tttgtcgtgc cgtccgccaa
    12061 acgtacgacg agaaaacctt ctccaccggg gccgctggca tacggttcta ccacgcccaa
    12121 ggcgaatccc atcgcgacgg ccgcgtcgac agcgttaccg cccatttcta gtactcgggc
    12181 gcctacctcg gcagcataag gactcgctga ggcaaccatg ccgttcatag cgatagccgt
    12241 ttgtccggta acgatttggc caaatacgga aaggctcaga acggagacga tcaacaggat
    12301 actggcaaaa cgtttcatgt ttccctcctt ctttcgggta cataattcct tgaaacgccc
    12361 tgttcggatc cccttttcag gatctggtta gggcgccggt gctgacccga atctttcttt
    12421 attataccct ataagagggc gggatttacc cttcgggtaa ttcaggctta ccgaaaaact
    12481 ctataacccg cataaaaagt gtgaaggtct attttctttt gaccccttac gctcgagtag
    12541 taaccccttg tttt
//
LOCUS       DAOPXN010000062        11852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5528_length_11852_cov_5.500127, whole genome shotgun sequence.
ACCESSION   DAOPXN010000062 DAOPXN010000000
VERSION     DAOPXN010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11852
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5528_length_11852_cov_5.500127"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PKM49_04980"
     CDS             complement(<1..385)
                     /locus_tag="PKM49_04980"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82200.1"
                     /translation="MEKISKSHDKFFKEVLGDIETAKSFLQHYLPPKIVRLIDPESIT
                     IEKDSYVEKDLSEYFFDLLFRVKMQQEEGYVYLLFEHKSKVDKHVSLQLLGYMTSIWK
                     KTVPQPLPVILPVVFYQGREKWEAPQ"
     gene            506..1084
                     /locus_tag="PKM49_04985"
     CDS             506..1084
                     /locus_tag="PKM49_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HNY82201.1"
                     /translation="MSEFILAVDVSKIPDGGTLEIEGDPSLSEIETPTATLPLRAPLS
                     YKIRLSRNKEDFFLFGTVSTVLEYPCARCLESVDLAVSGQVEATYKKPPKRVVEEEIE
                     LEDLENVIYYEALTLDLSERVIEAILVEIPIKVLCKEECKGLCPYCGVDLNQVAEHHC
                     EIEASVSPGRLFNALKPFATEDRDTNDNKNDR"
     gene            1096..1296
                     /gene="rpmF"
                     /locus_tag="PKM49_04990"
     CDS             1096..1296
                     /gene="rpmF"
                     /locus_tag="PKM49_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNY82202.1"
                     /translation="MAVPKQKQSRTRTHRRRAETLYKAVETPLSKCPNCGELRKPHRV
                     CLHCGFYKGRQVLEFKEDKEEK"
     gene            complement(1313..1981)
                     /locus_tag="PKM49_04995"
     CDS             complement(1313..1981)
                     /locus_tag="PKM49_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DtxR family transcriptional regulator"
                     /protein_id="HNY82203.1"
                     /translation="MGEMFSESLEDYLLVIYEMEINGQVPRVKGISEALKKKSTSVID
                     ALKKLGASDLIEYERHSHIKITQKGISKAQRIFRRRMLFESFLSTILKIPAEASSDLA
                     MKLEHIDSDDFYRGLETLQLFFEREPDSFKKFESFSEERKKNPHTAYLTLNKVRLREK
                     VKVLEIKGSDFVKKRLVAMGFVPGVEVEIIGTAPMGDPIEVKIRGYNLALRKDEAANI
                     FVEK"
     gene            complement(3210..4901)
                     /locus_tag="PKM49_05000"
     CDS             complement(3210..4901)
                     /locus_tag="PKM49_05000"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82204.1"
                     /translation="MRHSRWISLTLTLLNEHYGISRKRQRYLVDRKDLWEPFLIFGIV
                     AVLIVTLFPLMKTLLDQMIAVYAQMGAPEMVYAYFFMICSAFGFLVGLLVILSVFFFS
                     KDLPLLMSLPLKPTEILSAKIALIVFDQTWISIVGLLFPYIYLGARIGLGWEYWISGF
                     LILLSAQILPILFETIVILPLSRVIRFGKHRDFLLFSVSVLFVVAILLINVAITNTGV
                     SGELTEETFREFLENPQNIILKGLSIYPPAIWAYRAVSAKGMVSLGWLACFLSVTAGG
                     FLLTLFLANRFYLASYLELQDRHTRKSLLTRAELDHSIRRERSPVRALFIREWRYFLR
                     IPAFAFNGIGNVLIFPILLILFGSTNKSLGSASSLGAIITNLESLRAYLLPLGTLVGV
                     LAGGMNLLSSTFLSREGRMLTELKTLPVSAKDILTAKYLHIFILSEIGPLTAVIAMRI
                     LVQAAWGDLLGAFIGSSVCISFLNLIQMIVDIKRPMLDWDNPQKAMKQNLNGLFSVVL
                     LFGFIAGCLGMGWLTQGFLSPLMMFALLVGVCALGHIPLIPITISSLDRFLLKDL"
     gene            complement(4855..5616)
                     /locus_tag="PKM49_05005"
     CDS             complement(4855..5616)
                     /locus_tag="PKM49_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82205.1"
                     /translation="MLEIRHLTKVFGKNFKAVDDLSLSVKKGEVFGFLGPNGAGKTTT
                     IKMIVGLLKPNEGSISIGGKDIQKDTIAAKLKIGYVPDEPVMMEKIKGIEYLDFIADM
                     YGVPLQKRRERVEKLTTAFKLRNALRDIVAGYSHGMKQKLSLVAALLHQPDLWILDEP
                     LTGLDPESAFLLKKMMQNHTESGNTVFFSTHLMEIAEKLCDRIGIIHQGKLLFTGTIE
                     ELKKEKGEATLERVFLEVTGSEAQQMDFSYLDASE"
     gene            complement(5630..6403)
                     /locus_tag="PKM49_05010"
     CDS             complement(5630..6403)
                     /locus_tag="PKM49_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82206.1"
                     /translation="MYSKEILDIRWRLLVFFILFGILLVGTIALRPFVGELLGISSRE
                     IDELPEFVRKIVGDLSALNRLETDDEYYLYSQWQGKNMGQFLPICALLLVIPLFCREE
                     EKKTLYFLLARFPRRKIFWSKIAASFSAILSLLVIFALAAPAMMNLAGFSVGFQNSFL
                     ILVQQVLALWFFFGFFVFLSVVFRDQLKPILWGILFVLALPMAGLLPRFSWINPYPYL
                     CGVSVLNTGGIDWAYSFGMMIIGAAFLGIAAILFEKKEF"
     gene            complement(6396..7265)
                     /locus_tag="PKM49_05015"
     CDS             complement(6396..7265)
                     /locus_tag="PKM49_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82207.1"
                     /translation="MGIHLEKGLLFDRVKKTYRNGVKAVDGLTLNIPKGCVHAFLGPN
                     GAGKTTSLKAALGFISYGGTVSWDGKPVEAMRHDIVFVPEEKRFYEHWTLVETLRICS
                     KLDKRFSLQRAREYFDFFELPMKKRIRGFSLGMKTSLYLSLAFSTEAGFLILDEPTSG
                     LDPIKRDDVLEIIRRQVIDGKTVLYTSHIIPEVERIADTFSILQHGTLVYNGTLDELK
                     ERFRVFTFSMEEWGREEAPKGVIVTEQRDQVTVLTDNEQAISAWNKKKECFSEIPTLD
                     TFFQLTIRGKRYV"
     gene            complement(7250..7615)
                     /locus_tag="PKM49_05020"
     CDS             complement(7250..7615)
                     /locus_tag="PKM49_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNY82208.1"
                     /translation="MPIDIRSPMPAYQQIKISVKEAIVTGRLKENDTLPSIREMAQEM
                     KINPNTVVRAYRDLEQEGIIFPKQGIGNLVIASADTVRQSLIEEMAAELFMTMAKLKK
                     TGILFQEFAKVVEKVWESI"
     gene            8158..8424
                     /locus_tag="PKM49_05025"
     CDS             8158..8424
                     /locus_tag="PKM49_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNY82209.1"
                     /translation="MMIKPLGDRVLIKPLKEETRTVGGIVLPDTSKEKPIKAEVIALG
                     KLEEETLSIGDQVIFSKYSGTELKMDNEEYILIDAKDILAIIVR"
     gene            8441..10069
                     /gene="groL"
                     /locus_tag="PKM49_05030"
     CDS             8441..10069
                     /gene="groL"
                     /locus_tag="PKM49_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103842.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNY82210.1"
                     /translation="MSKLLKFDEEARRALEKGMNVLADAVKITLGPRGRNVVLEKSWG
                     SPTITNDGVSIAKEIDLEDKFENLGAQLLKEVASKTNDIAGDGTTTATVLAQAMAKEG
                     LKNIAAGTNPIVMKKGIQMASDAAVAHIKKASKKLKTKADIENVASISANNDPETGKL
                     IANAMDKVGEDGVITVEDSKTMETFVDFAEGMKFDRGYISPYFCTNMEKMEVELKEPY
                     ILITDKKISAVQPLIPVLEKIAQSGKPLLIIAEDVEGEALTTLVLNKLKGTLVSTAVK
                     APGFGDRRKAMLQDIAILTGGTMISEEIGLTLENVTLGDLGEAGMIRVKKDDTIIIDG
                     KGQKADIQARIIQIKKQIAETTSEYDKENLQERMSKLSGGVGVIKVGAATETELKEKK
                     HRIEDALSATRAAVEEGVVTGGGVTLLHAKKAVMDLIGTLTIPEEKIGAMIVAKALEA
                     PTRQIVENAGVDGSIIIDKILNQKNPDFGYDALKNEYTDLMAAGIIDPTKVTRSALQN
                     ATSIVSLLLTTEVLIVDKPEPKTPPMPPAPDYDY"
     gene            10177..11697
                     /locus_tag="PKM49_05035"
     CDS             10177..11697
                     /locus_tag="PKM49_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HNY82211.1"
                     /translation="MRKHSVMLLVCLMALVSALWAAPQLLTILHVNDTHGHAWSYTLG
                     DLKNVGGFAAIATLVDQIKAEVEAKGGSVLVLHAGDINTGVPESDQLDAISDVVGMNM
                     IPFDAMVLGNHEFDKDGTVLAKQIKTMKFPALSANILTKTGEPVFTPYVIKQLKDLKV
                     AIYGLSTEAITILEPIYVEDYVFMNAIELSKKMVPELRAQADIVVALTHLGYGEPHPG
                     CTTSDMLAKAVDGIDVIVDGHSHTLFTEAPVYGDTILVQAGEWGKYLGRLDLWILDGK
                     VIDWEWEAIPVNSIAGVEHIEQNPRVLAAMEYFYNLGGEKLNEVIGKTAIMLDGERSN
                     ARAKATNLANLLGDAMVWKSMADFAITNGGGIRASIKAGDITYRDVLTVLPFGNMIYV
                     VNLTGSEVMRLLQFASGVPAGQGAFPQFGGITFTVENGQVKNVLIQGKPLEMDRVYKV
                     ATNDYMAGGGDGYTVLKDNKANGYDTGFVLADALMLYFQYLGTVQDYDSSQRMVKK"
BASE COUNT         3151 a         2991 c         2987 g         2723 t
ORIGIN      
        1 attgcggcgc ttcccatttc tctctcccct ggtagaagac cacgggtaga atcactggta
       61 ggggttgcgg tactgtcttc ttccatatgg aggtcatata ccccaggagt tgaaggctca
      121 cgtgtttgtc aaccttgctt ttgtgttcga acagcagata gacatacccc tcttcctgtt
      181 gcatcttcac ccgaaagagc agatcgaaga agtattccga gaggtctttc tcgacgtaac
      241 tgtctttttc tatcgttatc gattcgggat cgattaaccg gacgatcttc ggaggtagat
      301 agtgttggag gaaggacttc gcggtttcta tatcccccaa gacttctttg aaaaatttgt
      361 cgtgtgactt actgattttt tccacgggta tccttccaga agaaagtctc atcggtatta
      421 taccttaaag gcgggggttt ccaaaaaagc ccccgaaaat actcccgttg tgatataata
      481 cacactcgta tatggaggag agattatgag cgaatttata ttagcggtgg atgtatcgaa
      541 gataccggat ggagggaccc tcgagatcga aggcgacccc tcgctgtcgg agatagaaac
      601 cccaacggcg acactgccgt tgagggcgcc tttgagctat aagattcgcc ttagtcggaa
      661 taaagaggac ttttttctat tcgggacagt ttcgactgtt ttggaatacc cctgcgcgcg
      721 ttgtttagag agcgtggacc ttgcggtttc cggtcaggtt gaggccacgt acaagaaacc
      781 tccgaagagg gttgtcgaag aagagatcga gcttgaagat cttgaaaatg tgatatacta
      841 tgaggctttg actttagacc tctccgaacg ggtgatcgag gcgattctgg tagagattcc
      901 cataaaagtt ttgtgtaaag aggaatgcaa aggcctttgc ccatattgcg gagtggattt
      961 gaaccaggtg gccgagcacc attgcgagat cgaggcgtcg gtatcgcccg ggcggttgtt
     1021 taacgcgctg aagcctttcg cgacggaaga tagggataca aacgataata aaaacgacag
     1081 ataggaggaa agacaatggc tgttccaaaa cagaaacaat cgcggacgcg gactcacaga
     1141 agaagagcgg agacccttta caaagctgtc gaaacgcctc tttcaaagtg cccgaattgc
     1201 ggcgagttac gaaagcctca ccgggtttgt ctgcactgcg ggttttataa agggagacag
     1261 gtgctcgaat ttaaagagga taaagaagaa aaataaaacg gcgaaagccg ttttattttt
     1321 ctacgaagat gttagcggcc tcatcctttc gcaaagctaa gttataaccc cgaattttta
     1381 cctcgatcgg gtctcccata ggtgccgttc cgatgatttc tacctccacc ccgggcacga
     1441 agcccattgc cactaatcgc tttttgacga agtctgaccc cttgatctcg agcaccttga
     1501 ccttttcgcg caaccgaacc ttattgagcg tcaggtaggc ggtgtgcggg ttcttcttgc
     1561 gttcttcgga aaaggattcg aacttcttga aagaatccgg ctctctttcg aagaagagct
     1621 gaagggtctc caatcctcgg tagaagtcgt ctgaatcgat atgctccaac ttcatcgcca
     1681 aatcggatga agcctccgcc ggtatcttca aaatcgtgct caagaaggat tcgaagagca
     1741 tccgacgtcg gaaaatccgc tgggctttcg aaatcccttt ttgagtaatc ttgatgtgac
     1801 tgtggcgctc atactctatg agatccgaag cccccaattt ttttagggcg tcgatgacgg
     1861 atgtgctctt ctttttcaat gcctcgctaa tgcctttcac ccgtggcacc tgtccattga
     1921 tctccatttc gtatatcact aacagatagt cctctagact ttcggaaaac atctctccca
     1981 tcgcgtgccc tcctaactat cgcgttgcca gtcgtctaat cactcgtttc taaggtgaat
     2041 tatactacat ttaccaaaat taagtatacg agcctattta ttttcaacgg tcattggttt
     2101 tttggataat gcccgacgtc agcgtatttg taaaaagaaa gagagtaacg caccgtcttg
     2161 aagaaatggg tcgcttacgc cgaatgacgc gcttgggaat acctgggaac gccaactttt
     2221 aagttggcat gttttttaaa aaatggttct agcacggagc gtacagagga aacgaagaga
     2281 acaggacaca cgtcacacaa aagtgcggcg ttcccagagc gtacacaagc gtatcgcgcc
     2341 gtcgcgaaaa gaaggggcgc ttacgccgca atgaccttgt gggtaatcgc ggggtaaccg
     2401 aagggacccc gcgaaacgcc cttcctcttt ttttgattaa aaagtaccag aaaagataag
     2461 gcgggaaatt taaaaacgta tcgcgccgtc gcgaaaagaa ggggcgctta cgccgcgatg
     2521 ctgtcttcct tttttcacaa accatgccaa actaaagttt ggcgttccca ggcgtcccag
     2581 ggtgctcccg gggcgttcac aagcgtatcg cgccgtcgcg aaaaaaagag gcgcttacgc
     2641 cgcgatgctg cgcacgtaaa cgcactcagt gaagaaacca tgccaaacta aagtttggtg
     2701 ttcccaggca ccccaggctt tccagggcgt tcctagacgt tcacaaacgt atcgcgccgt
     2761 cgctaaaaga aggggcgctt acgccgcgat gcctgttttc tccttttcac aaaccatgcc
     2821 aaactaaagt ttggcgttcc caggcgttcc caagcgttcc caagcacccc aggctttcca
     2881 gggcgttcct agacgttcat aagcgtagcg ccgtcacgaa gagaaggagc gcttacgccg
     2941 cgctccgcgt tttctccgtg ccctctgtgt gaaaccgttg tatttctaag acatgccaac
     3001 ttaaaagttg gcgttcccaa gcgttcccag gcgccccagg ctttccaggg cgttcacaag
     3061 cgtatcgcgc cgtcgcgaga aaaaggggcg cttacgccgc gatgctgtct ttccattttc
     3121 gcaaaccatg ccaaactaaa gtttggcgtt cccagggcgt tcccaggcgt tcgcactcaa
     3181 agagcgccct tttcaccgcg agaaacgaat caaaggtctt ttaataaaaa acggtccaac
     3241 gatgaaattg tgatagggat taaagggatg tgtccgaggg cgcagacccc aacaagcagc
     3301 gcgaacatca ttaacgggct tagaaagcct tgagtcaacc accccatacc taaacagccg
     3361 gcgatgaaac cgaatagaag gaccacggaa aatagcccgt tcaggttctg cttcatcgct
     3421 ttttgggggt tgtcccagtc taacataggt cgtttgatgt cgacgatcat ctggatgagg
     3481 tttaaaaagg agatgcagac tgacgacccg atgaaagctc ctaagaggtc tccccacgcg
     3541 gcttgtacga gtatacgcat agcgatcact gcggtaaggg gcccgatttc gcttaatatg
     3601 aaaatatgga ggtacttcgc ggtcaagatg tctttcgcgc tgactggtag cgtttttagc
     3661 tcggtcagca tcctaccctc ccggctcaag aaggtcgatg agagcagatt cattcctcca
     3721 gccagaacac cgaccaatgt tcccaacggg agaaggtagg ctctcagact ttcgagattc
     3781 gtaatgatcg ctcccaaaga gctcgcgctc cctaaggatt tgtttgtgct gccaaatagt
     3841 attaggagga taggaaatat caacacattc ccgatgccgt tgaacgcgaa ggccggaatc
     3901 cgcaaaaaat atcgccattc acggatgaaa agcgcgcgta caggcgacct ctcgcgtcgg
     3961 atcgaatggt ccagctctgc gcgggttagg aggctttttc gggtgtgtct gtcttgcaac
     4021 tccagataag aggcgaggta gaaacggtta gctaaaaaca gggtcagtag aaaaccaccc
     4081 gccgtgaccg atagaaagca ggcaagccaa cctaaactga ccattccttt tgcgctcacc
     4141 gcccgatagg cccagatcgc gggcgggtag atagagagcc ctttcaaaat gatgttttgc
     4201 gggttttcta aaaattctct aaatgtctct tccgttaatt caccggacac cccggtattc
     4261 gttatcgcca cattgattag tagaatcgcc actacgaaca acacgctaac gctgaacagg
     4321 agaaagtcgc ggtgtttccc gaaacggatg acacgtgata agggcaagat gacgatcgtt
     4381 tcaaatagga tcgggaggat ctgcgcggac aggaggatca ggaaaccgga tatccagtat
     4441 tcccagccga gtccgatgcg tgctcccaag taaatgtagg ggaaaagtaa accgacgata
     4501 gatatccacg tttggtcgaa gacgatcagc gcgatcttcg ccgagagaat ctccgtcggt
     4561 ttcaaaggta gcgacatcag caggggtaag tctttagaga aaaagaagac gctgagaatc
     4621 acgagcaagc cgacaaggaa cccaaaggcg ctgcagatca tgaagaaata ggcgtagacc
     4681 atctccggcg cgcccatctg ggcatagacg gcgatcattt ggtccaacaa cgttttcatc
     4741 agcggaaaga gggtgacgat taaaacggcg acaattccga atattagaaa gggttcccac
     4801 aggtccttcc ggtcgaccag atagcgttga cgtttccggc tgattccgta gtgctcattc
     4861 agaagcgtca aggtaagaga aatccatctg ctgtgcctca ctgcccgtca cctccagaaa
     4921 aacgcgttcc aacgtggctt cccctttctc tttcttcaat tcttctattg tccccgtgaa
     4981 caggagcttt ccttggtgaa tgatgccgat tcgatcacaa agcttctcag cgatctccat
     5041 aagatgtgtg gagaaaaaga cggtgtttcc gctttcggta tggttttgca tcatcttttt
     5101 caacagaaaa gcagattccg ggtccaaacc cgttaaaggt tcgtccagta tccaaaggtc
     5161 cggctggtga agcaacgccg caacgaggga gagtttttgc ttcattccgt gcgaataacc
     5221 ggctacaatg tcacgcaagg catttcgcag tttaaaagca gttgtgagct tttcaacgcg
     5281 ctcacgacgt ttctgtagcg gaaccccata catatcggcg atgaaatcga gatactcgat
     5341 ccctttgatc ttttccatca taaccggttc gtcgggcaca tagccaattt tcagtttggc
     5401 cgcaatcgta tccttttgga tatcctttcc cccgatcgat atcgatcctt cgttgggttt
     5461 gagcaaaccg acgatcattt tgatcgtcgt tgtctttccg gcgccattgg gtccgaggaa
     5521 cccgaaaacc tctcccttct tcaccgacag ggacagatcg tcgactgctt tgaagttttt
     5581 accaaagact ttcgtgaggt gtcgtatttc taacatgctc ttctccttcc tagaactctt
     5641 tcttttcgaa tagaatagcg gcgataccta gaaaagccgc cccaataatc atcatcccga
     5701 acgaataggc ccaatcgata ccgccggtat tcaaaacaga taccccgcaa aggtaggggt
     5761 aagggttaat ccaggaaaac cgaggaagca aaccggccat cggtagcgcc aaaacgaaaa
     5821 ggatccccca taagatcggt ttgagttggt ctcgaaagac cacggataga aaaacgaaga
     5881 atccgaagaa gaaccacagg gccagaactt gctgtacgag tatcagaaag ctgttttgaa
     5941 aaccgactga gaaacccgcc aagttcatca ttgcgggcgc cgcgagggcg aagataacca
     6001 ataaggagag aatggccgaa aacgatgctg cgatcttgct ccaaaagatt tttcggcgtg
     6061 ggaagcgcgc aaggagaaag tacaaagttt ttttctcttc ttctcggcag aaaaggggga
     6121 tgacgagcaa gagcgcgcat atcgggagaa actgtcccat attcttccct tgccattggg
     6181 agtataggta gtattcatca tcggtttcca agcggttcaa ggcggaaaga tcccctacga
     6241 ttttcctcac gaactcgggc agctcgtcga tttctcggct tgagatgcca agcagctctc
     6301 cgacaaaagg acgcagggcg atggtgccga caagcaggat gccaaagagg atgaagaata
     6361 ccaggagccg ccatcgtata tccagaatct ccttgctata catatctctt cccccttatc
     6421 gtcagttgga agaaggtgtc tagcgtgggg atttcactga agcactcctt cttcttattc
     6481 caagcggaga tggcctgttc attgtctgtc aagaccgtca cctggtctcg ctgttccgtc
     6541 acgattaccc ctttcggcgc ttcctcgcgt ccccactcct ccatcgagaa ggtaaagact
     6601 cggaatcgtt cttttaattc gtccaacgtg ccgttgtata ccaaagttcc gtgttggagg
     6661 atcgaaaagg tgtcggcgat cctctccact tcagggatga tatgagaggt gtacaaaacg
     6721 gtcttgccgt cgataacctg tcgacggata atctctaaca catcgtctct tttgatcgga
     6781 tccaacccag acgtcggctc gtctagtatg aggaacccgg cttctgtgct gaacgccaaa
     6841 ctgagataga gcgaggtttt catacctaaa gagaagccac ggatcctctt tttcatcggt
     6901 agttcgaaga agtcgaaata ctctcgcgct ctctgtaagc tgaaacgctt gtccagttta
     6961 gaacaaattc gaagcgtttc gacgagggtc cagtgttcgt aaaatcgctt ctcttccggt
     7021 acgaaaacga tgtcatggcg catcgcttct accggtttcc cgtcccaact caccgtgcct
     7081 ccataggata tgaaccccaa agccgctttc aacgatgtgg tttttccggc gccattgggt
     7141 ccgagaaagg cgtgcacgca tcctttcggg atgttgaggg ttagcccgtc tacggctttt
     7201 acgccgtttc tataagtctt tttcacacga tcgaacagaa gtcctttttc taaatggatt
     7261 cccatacctt ttccaccacc ttcgcaaact cctgaaagag tatcccggtc ttttttaact
     7321 tcgccattgt cataaaaagc tcagcggcca tttcttcgat caaggactgt cgcacggtat
     7381 cggcagacgc gatgaccaag ttcccaatgc cctgctttgg aaagatgatg ccctcttgtt
     7441 ccaggtcgcg ataggcgcga accacggtgt tgggattgat cttcatctct tgagccatct
     7501 cgcggatcga tggcaaggta tcgttttctt tcaatcggcc ggtaacgatg gcctctttga
     7561 cgctaatctt aatctgctga taggccggca taggggaccg gatatcgata ggcatccaca
     7621 atcctctcac ctctcttatc tgtattatat catatagtac agttgatgtc aacaccaatg
     7681 aggggataaa gtcgaggcgc gacctgggaa gaccaacttt tagtacatcc aggtaagccg
     7741 gaaatgtcac aggggggaaa gcaagtacgc tggctgggtg cgggaggcac taggttcatc
     7801 gcggggtaac caaggggacc ccgcgatact aacgaaatcc agcgaggcgc tctttcctgt
     7861 ctttagcttt taagacctgc caacataaaa gttggcgttc ccaggcgttc ccaatagacg
     7921 tgccgaactg aagtgaggtg tttccaggcg ttcccagcag aatagtacga tatgaagaga
     7981 gatagtgcat attcgcgtaa aatggaggaa tatcgagtat tatccataca atctataata
     8041 tatctatatt atcggataat atcgccaatt caagcggttg ataatccgaa gcgttttttg
     8101 tagaatacgt tagcaatcaa actataagag tgctaatttt taggaggtgt ttttttcatg
     8161 atgatcaaac cattaggcga cagagtcctt attaagcctt tgaaagaaga aaccagaacc
     8221 gtcggaggaa tcgtgcttcc cgataccagc aaagagaagc cgattaaagc cgaagtcatc
     8281 gcgttgggaa aacttgagga agaaacgttg agcattggcg atcaggttat cttttccaag
     8341 tactccggga ctgaactcaa gatggacaac gaagagtaca tcctcatcga cgccaaagac
     8401 attctagcca tcatcgttcg gtagtggggg agagatagag atgtcgaaac tgttgaaatt
     8461 tgacgaagag gcgagaagag ccctggaaaa agggatgaac gtcttggccg atgccgtgaa
     8521 gataaccctg ggaccgagag gccggaatgt ggtcttggag aaaagctggg gaagccccac
     8581 gatcaccaac gacggtgttt ccatcgctaa agagatagac ctcgaagaca agttcgaaaa
     8641 cctcggcgct cagctcttga aagaagtcgc ctccaaaacc aatgacatcg caggcgacgg
     8701 cactacgacc gccaccgtcc tcgcccaagc gatggcgaag gaagggctca agaacatcgc
     8761 ggccggaacg aacccgattg tgatgaagaa agggattcaa atggccagcg acgctgcagt
     8821 tgcccatatc aagaaagcga gtaaaaaact aaaaaccaaa gcggatatcg aaaacgtcgc
     8881 ttctatcagc gcgaacaacg atccggaaac cggcaaactc atcgccaacg cgatggataa
     8941 agtgggagaa gatggggtca tcacagtcga ggattccaaa acgatggaaa ctttcgtgga
     9001 tttcgccgaa gggatgaaat tcgaccgcgg gtatatctct ccctacttct gcaccaatat
     9061 ggagaaaatg gaagtcgaac tcaaagagcc ttacatcctc attaccgata agaagatttc
     9121 ggctgtccag ccgctcatcc cggtcctgga aaagatcgcg cagagcggaa aacctctctt
     9181 aatcatcgcc gaagacgtcg aaggggaagc gctgaccact ctcgtgctga acaaactcaa
     9241 agggacgttg gtttcaaccg cggtgaaggc gcccggattc ggtgacaggc gaaaagcgat
     9301 gctgcaggat atcgcgatcc tgaccggcgg taccatgatc agcgaagaga tcggcttgac
     9361 gctcgagaac gtgaccctcg gtgacctcgg agaagccgga atgatccgcg tcaagaagga
     9421 cgacaccatc attatcgacg ggaaaggtca gaaagccgat atacaagccc ggattatcca
     9481 gataaaaaaa caaatcgccg agacgacctc cgagtacgat aaagagaact tgcaggagag
     9541 aatgtccaaa ctcagcggcg gcgtgggcgt catcaaagtc ggcgcggcca ctgagacaga
     9601 gctgaaggag aagaaacacc ggatagaaga cgccctcagc gcgactcgag cagccgtaga
     9661 ggaaggcgtt gtcaccggag gcggcgtgac cttgctgcac gcgaagaagg ccgtgatgga
     9721 cctgatcggc accttaacca tcccggaaga aaaaatcggg gcgatgatcg tggctaaagc
     9781 cctcgaagcc cccacccgcc aaatcgtgga aaacgcgggc gttgacgggt cgatcatcat
     9841 cgacaagatt ttgaaccaaa aaaaccccga tttcggctac gatgcgctga agaatgaata
     9901 caccgatctg atggccgccg ggatcatcga cccgacgaaa gtgacacgta gcgccctcca
     9961 aaacgccacc tccatcgtgt ccttgttact taccacggag gtcttgatcg tcgacaaacc
    10021 cgaacccaag acacctccta tgccccccgc ccctgactac gactactaaa aaaccacccc
    10081 ccccgcaagg gggggttttt acaaagaccc cttcccagaa ttgtagaaat ctgtgctata
    10141 atccttaaag aaactcaacg aataggaggg aaaaagatga gaaaacactc tgtaatgtta
    10201 ctcgtgtgcc ttatggcgct ggtaagcgcc ctttgggccg cgccccaact cttgaccatt
    10261 ctccacgtca atgacactca cggtcacgct tggtcgtata ccttggggga tctcaagaat
    10321 gtcggcggct ttgccgcgat cgccacactc gtcgatcaaa tcaaagccga agtggaagcc
    10381 aaaggcggtt ctgtgctggt tctccacgcc ggtgacatca ataccggtgt ccccgaatcc
    10441 gaccagctgg acgcgatctc cgatgtcgtc gggatgaaca tgatcccatt cgatgcgatg
    10501 gttcttggga atcacgaatt cgacaaagac ggcaccgttc tggccaaaca gattaagacg
    10561 atgaagtttc cggccttgag cgccaacatt ttaacgaaaa ctggcgaacc cgtgttcact
    10621 ccttatgtga tcaagcaact gaaggatctg aaagtcgcga tctacggatt gagcaccgaa
    10681 gcgatcacca ttctggaacc gatttacgtc gaagactatg tgtttatgaa cgccatcgag
    10741 ctctccaaga aaatggtccc agagctcaga gcacaagctg acatcgtggt cgctttaacc
    10801 cacttgggtt acggcgaacc ccaccccggc tgcaccacct ccgatatgct cgccaaagcc
    10861 gttgatggca tcgatgtgat cgttgatgga cacagtcata cgctgttcac tgaagcgccg
    10921 gtttacggag ataccatcct cgtgcaggct ggcgaatggg gcaagtacct gggtcgactc
    10981 gacctgtgga tcctcgacgg taaggttatc gattgggaat gggaagccat tccagttaat
    11041 tccatcgccg gcgtagaaca cattgagcag aacccaagag tgctggccgc tatggagtat
    11101 ttctacaatc tgggcggaga aaaactgaac gaggtcatcg ggaaaactgc gatcatgctg
    11161 gatggcgaac gcagtaacgc gcgcgcgaaa gccaccaacc tcgcgaacct gttgggcgac
    11221 gcaatggtct ggaagagcat ggccgatttc gcgatcacaa acggtggcgg tatccgcgct
    11281 tcgataaaag ccggcgacat cacctaccgc gacgttctca ccgttctgcc gttcgggaat
    11341 atgatctacg tggtcaacct cacgggttcc gaagtgatga gacttctaca gttcgcgtcg
    11401 ggcgttcccg cgggtcaggg cgccttcccg cagttcgggg gcatcacctt caccgtcgaa
    11461 aacggtcaag tgaaaaacgt tctgattcaa gggaaacctc tggaaatgga cagggtttac
    11521 aaagtggcga cgaacgacta tatggccgga ggcggagacg ggtacaccgt gctgaaagac
    11581 aataaggcga acgggtatga caccggtttc gttttagccg acgcgctgat gctctacttc
    11641 caatacttgg ggactgtgca agactacgat tcttcccaga gaatggtcaa aaaatagggc
    11701 cctattcgaa aagcctttct ttgcgccaag agccggttgc gacagcaatc ggctcttctt
    11761 cttgtttctc ccgaatataa aatctcgagc agttcagaac ccaactttcg actaaaagtg
    11821 ctacaaacgg cgataaaagc cactttctca aa
//
LOCUS       DAOPXN010000063        11611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5660_length_11611_cov_5.716424, whole genome shotgun sequence.
ACCESSION   DAOPXN010000063 DAOPXN010000000
VERSION     DAOPXN010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5660_length_11611_cov_5.716424"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1304
                     /locus_tag="PKM49_05040"
     CDS             54..1304
                     /locus_tag="PKM49_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY82212.1"
                     /translation="MYGGTGLGLSISGLIVEKMGSKILFESRVGEGSRFYFTLRVRSE
                     ELREHRAPAPQSIRRILAVDDNVNNLRILEGLLKSWGFDVALCDSGSAALDLLEKQGP
                     FDLMILDHLMPEMDGLQVALLIRQNPRWSANSLPIILLHSSAVDLSSFSDLSQQCVQC
                     ILTKPVKPDELRRCLDHFSGMTIHAEGERSPLDARGGNALARSDSVAVLLVEDQPLSR
                     QLLHSYISIAFPNAQIIDASNGREAVMAVQNQRFNAIVMDIRMPEVDGLEATRQIRAW
                     EAGKGYRTPIIAVTASAMKGDRERCLEAGMDDYLTKPVLQDSLSVALEKYIRQESRIE
                     PERVKVIAGLLDELTGALSSHDLRSLSLLKSLQEATSIEMRDDLFETLVEKINDLQFT
                     EALSLIKRLRQRWLYDETDDPHRG"
     gene            1273..2187
                     /locus_tag="PKM49_05045"
     CDS             1273..2187
                     /locus_tag="PKM49_05045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82213.1"
                     /translation="MMKQTILIVDDIPHNIHLMNAILNDEYRLLFATNGPDALKIAKD
                     WLPDLILLDVMMPEMNGFEVCRLLKQDDLLKDIPVIFVTALSEVVKESEGLKLGAIDY
                     IRKPYNPDIIRLRVRNQLELKRARDELFFLSNVDRLTGIPNRRALDASLEKEMKRAVR
                     THRSIGLLMIDIDYFKDYNDHYGHVSGDDCLRRIAEAFRENLLRPGDMVGRYGGDEFL
                     CLLPEIPRDGILFVAEKLRKAVDGLAIPHAYSAASDKVTISIGALWKMPGKEDTVRSL
                     IEAADKALYQTKQAGKNGVTLTLESEEQ"
     gene            2218..2820
                     /locus_tag="PKM49_05050"
     CDS             2218..2820
                     /locus_tag="PKM49_05050"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY82214.1"
                     /translation="MIPDRETALSLLNAAYEKNPGLWRDHSIVTSQVAYRIASAIPDM
                     DPDRCQMAGLLHDIGRIRGISGVRHTIDGYDYLMEQGDSDLARYCITHAFPGKRMIPH
                     TRWDFLPGQLDFVCGYLNGIEYDLTDKILQIADFLSTAHGPMVLEVRLMESALRNSVN
                     RKFLDFWRECLQLKTEIDQALGMSVYDLFDDLLIDWPNQG"
     gene            2836..3972
                     /locus_tag="PKM49_05055"
     CDS             2836..3972
                     /locus_tag="PKM49_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014732008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HNY82215.1"
                     /translation="MRLFQNLHAYGPEDLGKVDLLIGNGRVLSIGTKIDPFHLPDEDV
                     EVVRTDAWGATGEVIVVPGFFDGHVHLIGGGGEGGFGTRTPEGSPEMFFNTGTTSVIG
                     VLGTDGVTRDLKSLYGKVMQLRANGLNAFMLTGNYRFPPKTITGDVVQDIVLIREVLG
                     FGELAVSDHRGSGLTGGELKRLGLDCRVAGMISLKAGKLVLHIGDGAEGLASLFEATG
                     DRTLPPSQLLPTHISRNQRVLADGIRWIEERGGTIDFTAGNETPSLLASLSAPKHWPH
                     VTVSSDGLGSFPVFNEARECIRVDVAPVTGLMEAFKPLVLKKGLDISQALLPFTLTPA
                     RFFGLDQDGVGRIKKGGRADLLFLDEGLTLLATMSEGVFRYRRP"
     gene            complement(4236..6581)
                     /locus_tag="PKM49_05060"
     CDS             complement(4236..6581)
                     /locus_tag="PKM49_05060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNY82216.1"
                     /translation="MKRSVYYIGVRDLIESTLRSGSLDNSFFSPNRAVEGTHYHQKIQ
                     MSVGSNYRSEVPLSHRFVLRQHSIDIEMRGKADGVFETDEGAVIDEIKVLFGDARRVS
                     RDTFPNHWHQAMCYAFMWASAEKLQQIAIQLTYYFPDTKRTRRIKDFFTMEELTDFVK
                     PLVERYAERLAFCERWRAQRDASIRELDFPYPSFHKGQKELLDRVFASVRDRRTLFAQ
                     APTGIGKTLATIFPAIRALGEGYGEKVFYLTSKTSTQMVAESTFDLLRERGLKAKITV
                     ITAKDKVCFKETALCEPYYCEYADGHFDRVDAAIEALLPEKKINRSLIEDTAREYRVC
                     PYELTLDLALLSDIILCDYNYAFDPRVFLKRFFLSAGDYIFLIDEAHNLVDRARDMFS
                     AEIGLQQVETLCEAFQTADLEVYDATRGVGQRLRERESALEKRTDGPKAQTDLPKELI
                     KDMNTFLKAAEKWLETHYRSPLARPLIDLYFLASAFVKTGESFDEKFRVYDEATPEGF
                     RTKLFCLDPSTLLLEFLKKGRSAVFFSATLLPLDYFVRLFGGDENAERLTIPSPFPSE
                     NLCLLVNPGVSTFYRNRSSSYEKIVESIEALARSKKGNFLVFFPSHQYLQQVYESFAI
                     KNPDIDCIAQKSRMRESERQDFIEAFCEDREQTLVGFAVMGGIFGEGIDLRGDRLSGA
                     VIVGIGHPQICLEREIIKEYFAQTLETGYEYAYVYPGMIKVMQAAGRVIRSPEDRGVV
                     LLIDERFRYPSYQSLFPPEWSHARKVSSCSSLSQILKAFWK"
     gene            complement(7257..8327)
                     /locus_tag="PKM49_05065"
     CDS             complement(7257..8327)
                     /locus_tag="PKM49_05065"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNY82217.1"
                     /translation="MKNARIRFLTLLLLIIGLAVGTIVLAGQARQSNAKGLQEILSQG
                     FIKAPPEKRLIEAGKIEAEVKKGGYVTQKKSVGIVTGQVATEQLALALAQTQVSSTPS
                     GMVRVNRGRFQMGNVENDKSAFDWEKPVHTVTLTYDYWIGKYEVTFNEYDTYCAATGK
                     SKPSDAGWGRGTRPVINVSWWDAIGYCNWLSEREGLKKAYDGDGNLLDKSGKVTTDIT
                     KVEGYRLPTEAEWEYAARGGQNSRGYKYAGSNDLNEVGWYLDNSGKKTHPVGEKKPNE
                     LGLYDMSGNVMEWCHDRWDDSYYSQGPQTNPTGPSSGALRAVRGGSQSESAPYCRTAF
                     RIGGTSTVSGDKLGFRLSRTVF"
     gene            9079..9792
                     /locus_tag="PKM49_05070"
     CDS             9079..9792
                     /locus_tag="PKM49_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY82218.1"
                     /translation="MLKRLSSLGLLVLLALSAFSLNFTDYAVYNDIENGLKAAVLLEK
                     SVLMVFTLPNCSTCGRLKNEALSNPTVEALLREHCVVIVAEAEKDRYAKFPFSAYVNP
                     SSQSFETVNYYELITKKLGSNAVPFTIFLNPGLEMIGSTQSYNGYESYESALKTFIRL
                     NARNPKKVFKKITASEKELILDTLPYAQEIGWEEFKAAINNLDPLKVYILSKVKVEEV
                     RAVLDPLPLLRADVLIQTK"
     gene            9942..10715
                     /locus_tag="PKM49_05075"
     CDS             9942..10715
                     /locus_tag="PKM49_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNY82219.1"
                     /translation="MKHNETISLLLNRKSVRKFQETVPDLETIEAIVRAGQQAPFASQ
                     LYSVVYQSGGKYAFQAPLWFLICADIHKLRLFMKRRGWEIVTNGVTLLLFAIQDASFM
                     AENMVIAAESLGLGSCFLGECSTNANRMRALVKKHHLPKGVFPVVELVMGFPAEEYPP
                     RPRYPLDFTLFKDRYPELTEEGLTQAMAQMDDGYRAQGYYKNQNIKVKTPGREDTMSF
                     DEYGWTEHISRKWGQWEPSPDKLLEVLKEQGFAFSEEDE"
     gene            complement(10766..11041)
                     /locus_tag="PKM49_05080"
     CDS             complement(10766..11041)
                     /locus_tag="PKM49_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017895818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNY82220.1"
                     /translation="MNYREHLEELKKNPKFAKEYDKLGPEYEIIEQIIRARAEKGLTQ
                     QQLAEITGIPQAHISRIENGKYNPTLKQLKRLAKGLDKELHIEFKQV"
     gene            complement(11038..11388)
                     /locus_tag="PKM49_05085"
     CDS             complement(11038..11388)
                     /locus_tag="PKM49_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNY82221.1"
                     /translation="MWTVEYYETGKKSCPVKEFIDSLPSPKMQAKALRDIGLLESLGS
                     EMREPFSKPIGSGLFELRIQFSSNIARIFYFFFYGKRIVLTNGFMKKTNKTPQREIER
                     AIEYKNDYERRVRM"
BASE COUNT         2691 a         3063 c         2997 g         2860 t
ORIGIN      
        1 accaggacaa actgtttgtt gtctttacgt agggtgatga attcatcacg cgcatgtatg
       61 gtggaacagg cctcggattg tccatctccg gattgatcgt cgaaaagatg ggctcgaaaa
      121 tcttatttga gagccgtgtc ggcgaggggt cccgcttcta tttcacgctt cgcgtccgtt
      181 cggaagagct gagagaacac cgcgcgccgg cgccccaaag tattcgccgg atcctggcgg
      241 tggatgacaa cgtcaataac ctgagaatac tggaagggtt actcaagagc tgggggttcg
      301 acgtcgccct ttgcgatagc ggttcggccg cattggacct tttagagaaa caggggcctt
      361 tcgatttgat gattttggac catctgatgc cggagatgga tgggttgcag gtggccctac
      421 ttatacggca aaaccctcga tggtccgcaa acagcctccc gatcatccta ctccacagct
      481 cggctgtcga cttaagcagt ttttctgacc tgagccaaca atgtgtccaa tgtattctca
      541 cgaaaccggt caaacccgat gaattgcgcc gatgtttgga tcatttctcc ggtatgacca
      601 tacacgctga aggtgaacgg tctccactcg acgcgagggg agggaatgca ctcgccagaa
      661 gtgattctgt cgccgttttg ttggtcgagg accaaccgtt gagtcggcag ctcctacact
      721 cctacatatc aatagctttt ccgaatgccc aaatcattga tgcctcaaac gggcgggagg
      781 cggttatggc agttcagaat caaagattca acgcgatcgt gatggatata cggatgccgg
      841 aggtagatgg tttggaggcg acgcgccaaa tccgcgcatg ggaggcgggg aaaggatatc
      901 ggacgccgat catcgcggtt acggcctccg ctatgaaggg agaccgagaa cgatgcctgg
      961 aggccggcat ggatgattac cttaccaagc cggttttaca ggatagcctg tccgtcgctt
     1021 tagaaaaata cattcgacag gaatcgcgaa tcgaacccga gcgtgtaaaa gtcatcgcgg
     1081 gacttctgga cgaactcacc ggagcgctgt ccagccacga tctcagatcc ctttctttgt
     1141 tgaagagcct acaagaggcg acttctatag agatgcggga tgaccttttc gaaaccttgg
     1201 tggagaagat caatgatttg cagtttacgg aagcgctatc cctgataaaa cggctccgac
     1261 agaggtggtt gtatgatgaa acagacgatc ctcatcgtgg atgatattcc ccataacatc
     1321 catttgatga acgcgatcct aaacgatgaa tatagacttt tgttcgcgac gaacggtccc
     1381 gacgctttga agatagcaaa ggattggtta ccggatctga tcttactcga tgtgatgatg
     1441 cccgaaatga atgggttcga agtctgccgt ttgctcaagc aagacgacct tctgaaagac
     1501 attccggtca ttttcgttac cgcgctcagc gaagtcgtga aagaatcgga ggggttgaag
     1561 ctcggcgcga tcgattacat ccgtaagccc tacaacccgg atatcatccg gttgcgcgtt
     1621 cggaatcaac tcgaactgaa acgcgcgaga gacgaactct tcttcttgtc aaatgttgac
     1681 cgtctgacgg gtatacccaa ccgccgagcc ctcgacgctt ctctggaaaa agaaatgaag
     1741 agagccgttc gcacccatcg ctcgattggg ctactgatga tcgacatcga ttatttcaaa
     1801 gactacaacg atcactacgg ccacgtttcc ggcgacgact gcctcaggag gatcgcagaa
     1861 gcatttcgag aaaacttgct tcgacccgga gatatggtgg ggcggtacgg aggggatgag
     1921 ttcttgtgcc tgctgcccga aattccccga gacggcatcc tgttcgtcgc tgaaaagctg
     1981 cgaaaagcgg tcgatggctt agccatacca cacgcttatt ccgccgcatc ggataaggtc
     2041 acgatcagca ttggcgccct ctggaagatg ccggggaaag aagacaccgt gaggtccctc
     2101 atcgaagcag cggataaagc gctctaccaa accaaacaag ctgggaaaaa tggggttacg
     2161 ttgacacttg aaagcgaaga gcaatgaggg agaatacctc gaaatacagg aattcgcgtg
     2221 attccggata gagaaacagc cctttctctt ttaaacgccg cttacgagaa aaatcccggt
     2281 ctgtggagag atcacagtat cgtcacctca caagtggcct atcgtattgc gagcgcgata
     2341 ccggatatgg atccagatcg gtgccaaatg gcgggattac tccacgatat cgggcgtata
     2401 cgcggtattt cgggcgtgcg tcacacgatc gacggttacg actatttaat ggaacaaggc
     2461 gattcggacc tcgcccggta ttgcatcacg cacgcctttc cgggaaagcg gatgatcccg
     2521 catacgcgct gggatttttt accggggcag ttggacttcg tgtgcggata cttgaacggg
     2581 atagaatacg atctgaccga caagatcctc cagatcgccg attttttgtc gaccgctcac
     2641 ggtccgatgg tactcgaggt gcggttgatg gaatcagctt tacggaactc cgtcaataga
     2701 aagtttttgg atttctggcg agagtgcctc caattgaaaa cggagatcga tcaagcgctt
     2761 ggaatgagcg tgtacgacct gttcgacgac cttctgatcg attggccgaa tcaaggataa
     2821 ggagatgtga tccggatgcg actcttccaa aacctgcacg cctacggacc cgaagacttg
     2881 ggaaaggtcg acctactgat cggcaacgga cgggtcttgt cgatcggtac aaaaatcgat
     2941 ccgtttcatt tacccgatga ggacgtggag gttgtccgca cggacgcttg gggcgctacc
     3001 ggtgaggtga tcgtcgtccc gggtttcttc gacggtcacg tacacctgat cggaggaggc
     3061 ggggaggggg gattcggaac ccgtacgccg gaaggatccc cggaaatgtt ttttaacacg
     3121 ggcacgacga gcgttatagg agtcctgggt actgacgggg tcacccgaga cctgaaaagc
     3181 ctgtacggaa aagtgatgca acttcgagcg aacggattga atgcgttcat gttgaccgga
     3241 aactatcgat ttcccccgaa aacgatcaca ggcgacgtcg tccaagacat cgtgctgatt
     3301 cgcgaagtcc tgggcttcgg cgagctcgcg gtctccgacc acaggggctc ggggttgacc
     3361 ggcggagaac tcaaacgatt gggattggac tgccgcgtag cagggatgat cagcctcaag
     3421 gcggggaagc tcgttctcca catcggcgac ggggcggaag ggctcgcgtc gctattcgaa
     3481 gcgaccggcg accggacctt gccgccttcc cagctgctcc caacccacat ctcccggaac
     3541 cagcgcgtgc tcgccgacgg gatccgttgg atcgaggaac gaggtggaac catcgacttt
     3601 acggccggca acgagacccc ctcgctcttg gcgtctcttt ccgcacccaa gcattggccc
     3661 cacgtcaccg tcagtagcga tggtttgggg agctttcccg tattcaacga agcgcgcgaa
     3721 tgcattcgcg tggacgtcgc tcctgtgacg ggattgatgg aagcgttcaa accgctcgta
     3781 ttgaaaaaag gattggatat ctcgcaagcg cttcttccat tcacgctcac ccccgcccgg
     3841 ttcttcggat tggaccaaga cggcgtgggt agaataaaaa aaggcggccg ggccgacctg
     3901 ctcttccttg acgaagggct cactcttcta gccacgatga gcgaaggcgt ctttcgatac
     3961 cggagacctt gagccgccat ccgccgggat cctcttatgc aaaaaaggtt ccgaaacaac
     4021 agagcaaccc gtgagatcgt cacttcacga caaatggaag gccagcggcc ctgatgaagc
     4081 tcacgcgcga tcgttggcga aatcccttcg gtgcggagtt tcccactatt cgtctaccgt
     4141 aacatagcaa aaaacggttc ctgacctact cccttagaag agtacgagcg aaccaaccca
     4201 gcgctttccc tttacggggc attgagctat agacttcact tccagaacgc ctttagtatt
     4261 tgggatagtg aggaacaaga agaaaccttg cgagcgtgag accattcggg aggaaagagc
     4321 gactgataag aagggtatct gaagcgttcg tcgatcaaga ggacgacgcc ccggtcttcg
     4381 ggtgagcgaa tcaccctgcc tgccgcttgc atcaccttga tcatgcccgg gtaaacgtac
     4441 gcgtattcgt agccggtctc gagcgtttgc gcgaaatact ccttgatgat ctctcgttcc
     4501 agacatattt gtgggtgccc gatgcccacg atcaccgcgc cgctcagtct gtcacccctc
     4561 aggtcgatgc cctctccgaa aatgcccccc ataaccgcga acccgaccag cgtctgttct
     4621 cgatcttcac agaaggcttc gatgaaatcc tgcctttcgg attccctcat ccggctcttt
     4681 tgggcgatgc agtcgatgtc cgggtttttt atagcgaacg attcataaac ctgttggagg
     4741 tactgatggg agggaaagaa gacgaggaag ttcccttttt tggagcgcgc gagcgcctcg
     4801 atcgattcga cgatcttttc gtaagaggaa ctcctgtttc gatagaaagt ggagactccc
     4861 ggattcacga gtagacacag gttttcactt ggaaagggag acggaatggt cagccgctcg
     4921 gcgttttcgt cccctccgaa caagcggaca aaataatcca acgggagaag cgtcgccgag
     4981 aaaaacaccg cggaacgccc tttctttaag aactccaaaa gcagcgtgga ggggtccagg
     5041 cagaaaagtt tggtgcggaa gccttcgggt gtcgcctcat cgtaaacccg gaacttctcg
     5101 tcgaaggact ccccagtttt tacgaaggcg ctcgccagga agtacaagtc gatcaatggt
     5161 cgcgcgagcg gtgatcggta atgggtttcc aaccattttt ccgcggcttt cagaaaggtg
     5221 ttcatatcct tgatcagctc tttcggcaga tcggtttgcg ctttcggacc gtcagtccgt
     5281 ttttctaatg ccgactctct ttcacgtaat cgttgtccga caccccgcgt cgcgtcatag
     5341 acctccaaat ccgccgtttg aaaggcctcg cagagggttt ctacctgttg aagacctatc
     5401 tcggccgaaa acatatccct cgcgcggtca acgaggttat gcgcctcatc gatcagaaaa
     5461 atatagtcgc ctgcgctgag gaaaaaccgt ttgaggaaca ccctagggtc gaaagcgtag
     5521 ttgtagtcgc agaggatgat atcggagagc aaggccaaat cgagagtcaa ctcgtaggga
     5581 catacgcgat attcacgggc tgtgtcttcg atcagcgaac gattgatctt tttttccggt
     5641 aacagcgctt cgatggccgc gtctacccgg tcgaaatggc cgtccgcgta ttcgcaataa
     5701 tacggttcac agagcgcggt ttctttgaag cagaccttgt ctttcgcggt gatgacggtg
     5761 attttggctt tcaatccccg ttcgcgtaag agatcgaagg tcgattccgc gaccatctgg
     5821 gtgctggtct tcgaagtgag atagaagacc ttttctccat agccctctcc gagcgcccgt
     5881 atggcgggaa agatcgtagc cagcgtctta cctattccgg tgggcgcttg ggcgaacagc
     5941 gtcctccgat ctcgtacgct ggcgaagaca cggtcgagca actccttctg acccttatga
     6001 aacgagggat aggggaaatc gagttcccgt atcgacgcat cgcgttgcgc tctccaccgt
     6061 tcgcaaaaag cgagccgctc ggcgtatctt tcgactagag gtttcacaaa gtcggttagc
     6121 tcttccatcg taaaaaaatc tttaattctc cgcgtccgct tcgtgtccgg aaaataatag
     6181 gtcaactgaa tcgcgatctg ttggagtttt tctgcggatg cccacataaa cgcataacac
     6241 atcgcctgat gccaatgatt cggaaatgta tcccggctca ctcttctcgc atcgccgaac
     6301 aggaccttga tttcgtcgat aacggctccc tcgtcggttt cgaagacgcc gtccgctttt
     6361 cccctcattt cgatatcgat gctgtgctgt cgcaaaacga aacgatggga caacgggact
     6421 tccgaccggt agttgctacc gacgctcatt tggatttttt gatggtagtg cgtgccttcc
     6481 accgcacgat tcggggagaa aaaagagttg tctaaactgc ctgaacgcag cgtcgattcg
     6541 atcaaatcac gcacaccgat gtaatagacg cttcttttca agatgtttcc ttctccacaa
     6601 ggaacgattt tttaatcacg gtgttatttt accatcgatt aacctcgaat cacccatcga
     6661 actaaaacaa catacccgaa aggctcactg gagtgctgcg cttcagctcg gcaggggttt
     6721 gggaccgccg ccggaaccgc ctgggaacgc cgaacttcag ttcggcctgt ctttgggagt
     6781 gccctgggaa cgctgcactt ttgtgcggca cggatttggg accgccagct tttaagttgg
     6841 tatgtcttga aaaaaggttc cgcacagaga gcacagagaa agattcttgg gagcgccgaa
     6901 ctttagtttg gcatatgttt tacccaggag ctcttcactt tagagtggct tgggttcgat
     6961 tttctcttta ccctctgcgt tctctgtgtt ctcagtgcga acaatagtct ttgttggttt
     7021 gacgggacac ttccagacaa tttgtcgaac aaaagttcag cgtttccaga aaatatgcct
     7081 gcttaaaaat aggcgttccc agacgttccc ggcgctaatc tcccctccgc gttctctgtg
     7141 tgttctgtgt gaaccctttt cctcatttca tgttctaaac cgcttcgctt gttccgactt
     7201 ctttgagcgg aaaattaatc gctttcataa cctcgctaaa agtaaaaatc taaaagttaa
     7261 aagactgtcc tggaaaggcg aaagcctagc ttgtcgccgc tgaccgtcga cgtaccgccg
     7321 atccgaaacg ccgtgcggca gtacggcgcg ctttcgctct ggctgccgcc ccgaaccgct
     7381 cggagggcgc cgctgctcgg ccctgtcgga ttcgtctgcg gcccctgcga atagtaactg
     7441 tcatcccacc tgtcgtggca ccattccatc acgttcccgc tcatatcgta taaccctagt
     7501 tcgttcggtt tcttctcgcc taccgggtgt gtcttctttc ctgaattgtc caggtaccag
     7561 cccacttcgt ttaggtcgtt gctccctgcg tatttatacc cccttgagtt ttgtcccccc
     7621 cgtgccgcgt attcccattc cgcttccgtt ggcaatcgat acccttctac tttcgtgatg
     7681 tctgtcgtta cctttccgct cttgtccagc aggtttccat cgccgtcgta cgctttcttt
     7741 aacccttctc tctcactcag ccagttgcag tacccgatgg cgtcccacca gctcacgttg
     7801 atcaccggac gcgtcccgcg gccccagcct gcgtcgcttg gcttgctctt tcccgtcgcc
     7861 gcacagtatg tgtcgtactc gttgaacgtc acttcgtact tcccgatcca gtagtcgtac
     7921 gtcagggtta ccgtgtggac cggtttttcc cagtcgaacg ctgatttgtc attctccacg
     7981 ttccccatct ggaagcgtcc gcgattcacc cgtaccatcc ccgacggcgt gctcgatacc
     8041 tgagtttgcg ctaacgcaag ggctaactgc tccgtcgcga cttgccccgt tacgatcccg
     8101 accgatttct tctgtgttac gtaaccgccc tttttgacct ccgcttctat ctttcccgct
     8161 tcgataagtc ttttttccgg gggagccttt atgaatccct gtgacaagat ttcttgtagt
     8221 cctttggcgt tggattgtcg ggcctgacct gccagcacta tggtgccgac agctaaccca
     8281 attatcaata gcagtagcgt gaggaatctt atccttgcgt ttttcatttt ttcttcctcc
     8341 tctctttttt aatgattttt ccgttcgctt tctgtactaa attactcgcg attatcgagg
     8401 ttattctgcg gtttttcttg agcggatccc tttggttgtc ttatcccgat tttctggcac
     8461 acctcctttc cggcgcggat cgcgaggttt ccaaagttca ctcccggtag ctcgcgcagc
     8521 cattgcagtg taagcgcttc ttcttttcgc gacggcgcga tacggcggtt ttccctcttt
     8581 tccaaagatc aaaaaaacaa ggggcacaca gagggcccag agaaaagaat tttgggagcg
     8641 cttagggaac gccgaacttt tgttcggtat gggtttggga acgccaactt taaagtcaat
     8701 accgtctaat tgtaccacaa tcgcgtgtgt attttccgaa ggggggctcg aaagtgaagt
     8761 gccaaagcga atcgcggagg tacctgtaaa aatgagaatc ctgcgcggtc atcgcggtgt
     8821 aggcgctatg gacaaaggcg gatcgtgggg tggcaataac cgcttacccc gcgataaccc
     8881 cataggctca tcgcggcgtc taaaaaaaca aaattcgtat cgcgagactt cgcgggcttc
     8941 cgaagtcacc ccgctggtta ccccgcgatg aacctcttag ttatgtcacc ccataatttg
     9001 actaacacac cgcgccagaa agccgaaatg cggttcgtgg tataatcccg gaagacttta
     9061 tttttggagg gactgggtat gttgaaaagg ctttcttcgc taggcttatt ggttctcctt
     9121 gctctcagtg cgttctcgct caacttcacc gactacgctg tctataacga catagaaaac
     9181 ggactaaaag ccgccgtttt gttggagaaa agtgtgctga tggtgtttac gttaccgaat
     9241 tgctccacct gtgggcggtt gaagaacgaa gccctttcga accctaccgt agaagctctt
     9301 ttgagagaac actgcgtcgt gatcgtggcg gaagcggaaa aagaccgata cgcgaaattc
     9361 ccgttcagcg cctacgtgaa cccctccagc caatccttcg aaacggtcaa ctactacgag
     9421 ctgatcacga aaaaactcgg gagtaacgcg gttcccttta cgatatttct taaccctgga
     9481 ctggaaatga tcggatccac ccagtcctac aacgggtacg agtcttatga gtcagcttta
     9541 aaaacgttca ttcggctgaa cgcacgaaac ccgaaaaagg tatttaagaa aatcacggca
     9601 agcgaaaaag agctgatatt ggacaccctc ccgtacgcgc aagaaattgg ctgggaggag
     9661 ttcaaagcgg cgatcaacaa tttggaccct cttaaggtct atatcctcag taaagtcaaa
     9721 gtggaagagg tacgggcagt tttggacccg cttcccctac tccgggccga cgtattgata
     9781 caaaccaaat gaaatgagcg aaaaaccaca gggccctcta cacgggcagc ggactttttg
     9841 atatcatgta gaatgtgaag tagagaaata aattccacgg cttaacccgg aaacctggcg
     9901 gccctgaaaa atgcgtgaag gtgttgttgg aggcgatagg tttgaaacac aatgaaacga
     9961 tctctttatt attgaaccga aaatccgtcc gaaagtttca agaaacggtg ccggatctcg
    10021 agaccataga ggcaatcgtc cgagcgggac agcaagcgcc ttttgcgtcc caactgtata
    10081 gcgtcgtcta tcaatctggg ggaaaatacg cctttcaagc gcccttatgg ttcttaatct
    10141 gtgcggacat acacaaattg cggctgttta tgaaacgaag gggctgggag atcgtcacca
    10201 acggcgtcac cctcctgctc ttcgcgatcc aggacgcctc ctttatggcg gagaacatgg
    10261 tgatcgcggc agaaagcttg gggttaggct cttgtttctt gggagagtgc agcaccaacg
    10321 cgaatcgtat gcgcgctttg gtgaagaaac atcatctccc gaaaggcgtc tttcccgtgg
    10381 tcgaactcgt gatggggttc ccggctgaag agtatccccc gcgaccacga tatccattgg
    10441 acttcaccct attcaaagat cggtatccag agctcacaga agaggggttg acgcaggcga
    10501 tggcacagat ggatgatggg tatcgcgcgc aagggtacta caaaaaccaa aacatcaaag
    10561 tcaaaacccc aggtagagaa gacacgatgt ccttcgacga gtacggatgg accgaacata
    10621 tctcgagaaa atggggccaa tgggaaccct ctccggataa actcctcgaa gtcctgaaag
    10681 aacagggctt cgccttctca gaagaagacg aataaacgcc acgcgttaca ccgtagggag
    10741 ttcttttcat agtcgaagag aaagcctaaa cctgcttaaa ttctatatgc agctctttgt
    10801 cgaggccttt cgccaggcgc tttaattgtt tgagggtggg gttgtatttc ccgttttcaa
    10861 tccggctgat gtgggcttgc gggatgccgg ttatctccgc gagttgctgt tgcgtaaggc
    10921 ctttttcggc tcgcgcgcgt ataatctgct cgatgatctc gtactccggt ccgagtttgt
    10981 cgtattcttt agcgaacttc ggattctttt tcaactcctc caaatgttca cgataattca
    11041 catcctcact ctcctttcgt aatcgttttt atactctatc gctctctcaa tctctctctg
    11101 cggtgtttta ttcgtctttt tcataaaccc gttcgttaaa acgattctct taccgtaaaa
    11161 gaaaaaatag aatatcctcg cgatattgct ggaaaactgg atgcgtaatt caaagagccc
    11221 gcttcctatg ggtttggaaa agggttcgcg catttccgat cccaagctct ccaacaatcc
    11281 gatatcccgc aacgctttcg cttgcatttt cggagacgga agggaatcga tgaactcttt
    11341 cacagggcaa gactttttac ctgtttcgta atactccact gtccacattt cacgggctcc
    11401 ttgtataggg tttctccatt atatgtcata taagacattt tgtcaatacc tggtcgtcgg
    11461 gagggttatg ttgcctcgaa tcggcgagcg ctttcgactg tcatcgaggc gcaagcgcgc
    11521 cttcttttcg cgacggcacg atacgttttt gagggagggc atcgcggcgt aagcgccctt
    11581 tcttttcgcg acggcgcgat acccctttca t
//
LOCUS       DAOPXN010000064        11608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5663_length_11608_cov_5.804899, whole genome shotgun sequence.
ACCESSION   DAOPXN010000064 DAOPXN010000000
VERSION     DAOPXN010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5663_length_11608_cov_5.804899"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1676)
                     /locus_tag="PKM49_05090"
     CDS             complement(240..1676)
                     /locus_tag="PKM49_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82222.1"
                     /translation="MCAIHIKRIRGHEYAYDVTSYWDKEQKKYRKKTVYVGKVLDKEK
                     GLYEKRSDRAGNVEERLRQSLILSFGDTYALSEFLEKLPLGRLIEKSLPEEGDTLKGL
                     LYYRILKESAMQYAQTWMEGNYARILYPKARLTSQSISEFLKTLGEEGVWRKFFQSYL
                     AQASGEEAAVIIDSTGLPNEIDIPISAFGCHGGEIERETRLLMVMDQASGMPLYFRYM
                     AGNIVDVSTLATTMMELEYYGVKTAFALIDAGYFSQENIGKLYQNRIAFLTRLPARRV
                     LYKELIRAHAQDLEKAENAVVYGKRVLYVKRIQVDLYDYEGYAYVVCDIHRKAQETTK
                     LLLEAKETALMPEEIEEALIFKGKFVIVSSREIPIEEVLPLCYMRQTAEALFGISKDS
                     LDLIPLRVHSIEAFRGYLMLNFMALILYLELKKALKGKYTVEGALSELRNLMCTIYEK
                     KAIVMEPTKRMKEIFSLLDIKVPLSLRV"
     gene            complement(1796..3595)
                     /locus_tag="PKM49_05095"
     CDS             complement(1796..3595)
                     /locus_tag="PKM49_05095"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HNY82223.1"
                     /translation="MRRVLKIVLLCALAGLFFSFSAFAQLHIFYGNLHAHTSYSDGAG
                     DPWEAYTHARDYGKLDIQGVTDHCHYLQYPLADGSMRLPKTFQAAREMNEDGRFLAIA
                     GFEWTLTGQGHITVYDTQHFTHRDESDLFQLYEWIYENDGIGSFAHPGVKYGDFYDFV
                     YDPKADQVMNLIELGNGSTYVSRCINEEYLERYERALVRGWHLGAAANQDNHQANWGS
                     ANTLRTGVFMESLTLENLYKALRARHTFATEDADVKIYFEGNGAMMGDILYDLDRVTF
                     RVEYSDPGDTILEAQAIYSGGVLTLPVQGDVWETTFSLSIDKPYEWVFIRVEQDDSNE
                     VITSPIWIQDSKRVYLFNTFSFPARIVRDQPFKYGYEIANLNEEERTILFEIKNDEGE
                     AFFQKTITLEPLGLFKEVVELRVPTKALTLYLDGLPYGDAGLEEVSFIAGIDTSHENY
                     FLKELEPLSNYLESLGGDTIPLKGSLNPAKLSNCHWLIMPLPPSDAMLPSFAVLTARD
                     VELFKQYVTRGGHFIFVLSKEKSLPAAIDSCKRFFEEVGVSVHLDEDGTALSDPENVF
                     TTKSGSHFLFLTWEEATDPNRISEVLKGLGHLW"
     gene            complement(3650..4894)
                     /locus_tag="PKM49_05100"
     CDS             complement(3650..4894)
                     /locus_tag="PKM49_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82224.1"
                     /translation="MKKVWITVTLLICAMMLFAAPVKVVVWYAQTGIYSPTLLELIDE
                     FNKLNEGKIVVEGVYSGSYEDTMQKLVASMVAGNLPHIAQIEQSRIGQFLDGDAFQNL
                     DEFINKDPEFKATLSDFFPRFLAANTFYGKLYGFPLNTSTPLLYINRDVFRAAGLDPN
                     KPPRTWEEVYEAAKAVKKLGPDYYGYRLGDDDWILESYAWQFGGDIVSEDGMKVLLDS
                     PGTIDAWKFFQKGLREGVFVYTIPEQGNAMDLSGKVAMVCRSTGSLAYMIQNAKFDLG
                     ATFMPYQVKKATPIGGANIYMFKNKPQNEKQAAWEFMKFVTATPNTKKWSLATGYMAS
                     RVSAYESPEIQELFKKDPRAEVTYLQLKESAVRRPYIGPYREIFNIMLSSWQTIMTDM
                     NANVENVLKTATEKAQKVLDEY"
     gene            complement(4914..5744)
                     /locus_tag="PKM49_05105"
     CDS             complement(4914..5744)
                     /locus_tag="PKM49_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82225.1"
                     /translation="MRKAPSLWIRIPLYIAIIVIMLLVNLPFIWMLVTAFKDESEVFT
                     LPPKFYPNFFDFRNFAGAMAQIPMGRYLFNSLFVALSVTLLQLLFNSFAAYGLSRLRF
                     KGRDFIFLILVGTLMVPPEVTLVPLYIIVKNLGMIDSYRVLIIPFMSSAFGIFLLRQF
                     FLGIPRELEEAAIIDGAGHLKIFLTIILPLTKPALWTMSLFTFIAHWNEYMWPLVSVS
                     DPKYQMIQVGISQFVSGWETRWTYRMAASTLVVVPIIIFFFFVQKQFVEGISVSGIKE
                     "
     gene            complement(5741..6619)
                     /locus_tag="PKM49_05110"
     CDS             complement(5741..6619)
                     /locus_tag="PKM49_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82226.1"
                     /translation="MRRKTREALTGWVFVSPALVTQILFIYFPLGWSFFVSFFRWNLI
                     RPMRFIGFDNYTSMFTSSDFWSSMSVTFIYVAVTVPVSILIGLLLALLVNLPWLKGKG
                     VFRTFFYIPVITSMAAAAVIWAWIFEGNVGLLNYFLSWFGAKSINWLSDPDYALIALM
                     IIGIWKRIGYNMVLFLAGLQVIPRTYYEAADIDGASTFSKFTSITWPLLSPTTLFVTV
                     MQFIASFRVFVSVSVITRGGPANSTRVITFYLYENAFAYLKFGYAAAISVVMFLMMVV
                     FTLIQFRFSKRRVHYA"
     gene            complement(6708..7790)
                     /locus_tag="PKM49_05115"
     CDS             complement(6708..7790)
                     /locus_tag="PKM49_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82227.1"
                     /translation="MGLVLENLVKTFRSDGAELRAVDRIWLEIPSGALITLLGPSGCG
                     KTTTLRMIAGFELPTDGIVRLEGEDITFLPPNQRNISMVFQSYALFPHLSVEENVSFG
                     LKIKRLSRDVVRKEARDMIDLVGLRGLEKRRPDQLSGGQQQRVALARSLVMKPKVLLF
                     DEPLSNLDAKLRESMRLEIRRIQQEMKITSVYVTHDQVEAMSISDLIVIMDKGKIMQK
                     GSPFEVYTRPANAFVADFIGKVNFIDGVVDSRDGETCIVHAKTLNQSFKCRNLLVAEG
                     QGARIVVRPEAFSLTPSSNEGFNSISGIVRSFIFLGSHLEYSVELRDGSVINALEYNL
                     SEGSLPRVGEEKRLFFSQKNAWVIPG"
     gene            complement(7795..9480)
                     /locus_tag="PKM49_05120"
     CDS             complement(7795..9480)
                     /locus_tag="PKM49_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018526011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNY82228.1"
                     /translation="MKSKELFRSLKALTKEPFLLLLIIGIFFLLFLFSLYPLIKVAMV
                     SFEKGDGTLSLDVYRQTLSNNYLRQGFYNSLLVASLTAIFGVAIGYLFAYTLNRTNIP
                     LKRFFRFVALMPVVFPPFIGAMALIMLFGFNGLITAKVFGIRNFPVYGLWGLMMAQMV
                     CFFPVAFITLDGVISTISPTLEDAAFNLGASRWEAFRKVILPVSIPGIASTLLVLFIE
                     SLADFGNPLILAGSRFPVLSVQAYLEITGMYNLRTGAALAVWLLLPSILAFLAQKYWV
                     SRKRYVTLSGKPTTSVIKSVNRPIKWALFSLCLIISLFILLIYATIVWGSFTQAWGMS
                     SRFTLDNFKYVLDVGLESIKDTLVIAFSSTPLSGLLGMIIAFLIVRKRFPGKGAMDFL
                     SLLSFAVPGTVIGIGYIFAFNTPPFLLTGTLAILVLNFVFRYIPVGIQSGIALLNQVD
                     PAIEEAAFTLGANNRAVFRRITLPLIVPAFFSALVFSFVRAMTAISAAIFLVSAKWNL
                     MTVQILSQTDSGRLSEACAYSVILISIILIFMLSLKLFLRQRISLSNQNYGVR"
     gene            complement(9568..10551)
                     /locus_tag="PKM49_05125"
     CDS             complement(9568..10551)
                     /locus_tag="PKM49_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82229.1"
                     /translation="MKKCVFFLSLLLIALIGFSELVIYSSVDEANARKILNAFSAETG
                     IKLNFVFLSSGPAMARIEAEKNNPQADVWFGAPMENHILAKEKGLTMAYKSLHVYGIS
                     PEFYDVDGFFHAFYMNPLGIGINTQVLAQLKAPMPKTWEDLFHVAYRKMIQYPNPQAS
                     GTAYSMMTGLIMLYGEDGMIEFLKKMAPNVQTYTQSGTGPSKAVGTGQALMGLQFTPA
                     FFQFKEQGYPVEIIFPEEGVPYECACASILKGAKNIGEAQKLIDWLISKNGQQQIVEQ
                     KTYFYPIRNDVDFGSLQPLSTIKLITVDGIWAAQEKVRLLERWVKEVLPVQ"
     gene            complement(10767..>11608)
                     /locus_tag="PKM49_05130"
     CDS             complement(10767..>11608)
                     /locus_tag="PKM49_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82230.1"
                     /translation="SITIETDSYIEKDLSEYFFDLLFRVKMQQEEAYVYLLFEHKSKV
                     DKHVSLQLLGYMTSIWKKTVPRPLPVILPVVFYQGREKWEAPQWFSNRFSNSDSMDGD
                     LKDYIPDYKYELFEISSLREEEVKGAKRLRIYLDVIRMRALEGKEAIREVMLRVTVTI
                     SELPQTEANERFFQVCIIYLFDTMEREYFQQLSELMKTVSEERSEKMQTIADMLRQEG
                     IKKGREEGREELLWKQIAKKFPKASQKYFERLKSLTIEQLDSLGLELIDMKNEEELKK
                     HLM"
BASE COUNT         2965 a         2947 c         2763 g         2933 t
ORIGIN      
        1 cttacttcgc gtctggttgc ttattcgtct attatctgct tttcaaagac cgaaatacaa
       61 tgaccgaaaa cccggggcta tattatcata tctccgttgc tttgtcaatc ccttctcggt
      121 ctattgttcg cgtgaaaaaa atgttaatcg gtgttctatg gatcgaaccg ttccctacga
      181 catcggaaag ggtcccggtg aagcgttctc ttcaaaaatg tctacggtgc gctcttaccc
      241 tatactctca aagatagagg taccttaatg tcgagtaaag aaaaaatctc cttcattcgt
      301 ttggtcggtt ccatcacgat cgctttcttt tcgtaaatgg tacacatcag attcctcagc
      361 tccgagaggg caccttctac ggtatatttc ccctttagcg ctttcttcag ctccaaataa
      421 aggatcaaag ccataaagtt caacatcaga tacccgcgga atgcctcgat cgaatgaacc
      481 cgcaagggta tgaggtccaa agagtctttc gaaatcccaa aaagtgcctc tgcggtctga
      541 cgcatatagc acaacggcaa tacctcttcg atcggtatct cccgagaaga tacaatcacg
      601 aatttccctt tgaagatcaa cgcttcctct atctcctcag gcatcaacgc cgtttctttc
      661 gcttccaata aaagtttcgt cgtctcctgc gctttcctgt gtatatcaca gaccacatag
      721 gcatatccct catagtcata gaggtccacc tgtattcttt tgacatagag aacgcgtttg
      781 ccatacacaa cagcgttttc ggccttctcc aaatcctgcg cgtgggcgcg tatgagctcc
      841 ttgtaaagta cccttcgtgc aggcaagcgt gtcagaaagg ctatacggtt ctgatagagc
      901 tttccgatgt tttcttggct gaaatatccg gcatcgatta acgcgaaagc cgttttaacc
      961 ccataatact ccagctccat catcgtcgtc gccaaggtgg agacatccac gatattgccc
     1021 gccatatacc gaaagtacag gggcattccg ctcgcttgat ccatgaccat caataatctg
     1081 gtctctcgtt ctatctctcc tccatgacag ccgaaagcgg atatcggaat gtcgatttcg
     1141 ttcggcagtc ccgtactgtc gatgatcacc gccgcctctt ccccactggc ctgtgccaga
     1201 taactctgga aaaacttcct ccatacccct tcttctccca acgtcttcaa gaactcgctg
     1261 atactctgtg aggttaaacg tgctttggga tacagaatgc gcgcgtaatt cccctccatc
     1321 cacgtctgag catactgcat cgcactttct ttcaatatcc ggtagtatag caaacccttg
     1381 agcgtgtcac cctcttcggg tagactcttt tctattagcc tacccaaggg aagcttctcc
     1441 aggaattcgg ataatgcata ggtatcccca aaggaaagaa tcaaggattg tcgaaggcgt
     1501 tcttctacgt tgcctgcgcg atcacttcgt ttctcgtata ggcctttctc tttgtctaaa
     1561 accttcccga catagaccgt tttctttcgg tacttcttct gttctttatc ccagtaggag
     1621 gtcacgtcat acgcatactc gtggccccgg attcttttga tgtggatcgc gcacattatc
     1681 ttacctcgat atatggtagt tgcgtaacta ccacatattg taccacatca tgcttatgtt
     1741 gtcaagtgta tatcaggttc tcctcctatg gtggttaatt atcgaaggat ttggcttacc
     1801 aaagatgccc gagccccttc aagacctcgg aaatacgatt tggatctgtc gcttcttccc
     1861 acgtcaaaaa gagaaaatga gaaccgcttt tcgtcgtgaa cacgttttca ggatcagaca
     1921 atgcggtccc atcctcgtct aagtggaccg ataccccgac ttcttcgaaa aagcgcttac
     1981 aagaatcgat cgcggctggt aagctcttct ctttcgatag gacgaaaatg aaatgtcccc
     2041 ctcgcgtgac atactgcttg aaaagttcga cgtctctcgc cgttaaaacc gcaaacgacg
     2101 gtaacatcgc gtcggaggga ggtaagggca taatcaacca atgacagttg ctgagcttcg
     2161 ctgggttgag agatcctttc agcggaattg tatccccgcc gaggctttca aggtagtttg
     2221 aaagcggttc gagctctttc agaaaatagt tttcgtgtga agtgtcgatc ccggcgatga
     2281 aagaaacctc ctcaaggccg gcatcgccgt aagggagtcc atcgagataa agggtcaagg
     2341 ctttcgtcgg gactcgaagc tcgacgacct ctttgaacaa ccctaagggt tccaaggtta
     2401 tcgttttctg aaagaaagct tctccctcgt cattttttat ttcgaacaga atcgttcgct
     2461 cttcttcgtt caggttcgcg atctcatagc cgtacttgaa aggctgatcc cggactattc
     2521 gagcgggaaa tgaaaaggtg ttgaacaggt agaccctttt cgaatcctgt atccaaatag
     2581 gtgaagtgat gacctcgttg gagtcgtctt gctccacccg tatgaaaacc cactcgtaag
     2641 gtttgtcgat cgacaggctg aaggtggtct cccatacgtc tccctgaaca gggagtgtca
     2701 agacccctcc tgaataaatg gcctgagcct cgagaatcgt atctccggga tcagagtatt
     2761 cgacccgaaa tgtgacgcga tccaggtcat agaggatgtc tcccatcatc gcaccgtttc
     2821 cttcgaaata aattttcaca tctgcgtctt cggtcgcaaa ggtatgacgt gctcttagcg
     2881 ctttgtatag gttttcgaga gtcagcgatt ccatgaaaac tcccgtgcgg agggtattgg
     2941 cggatcccca gttcgcctga tgattgtctt ggttggctgc cgcgcccaga tgccatcctc
     3001 tcactaaagc gcgttcgtag cgttcaagat actcctcgtt gatacaccga gaaacatagg
     3061 tgctcccgtt ccctaattcg atgaggttca tcacttggtc cgctttcggg tcgtatacga
     3121 agtcgtaaaa atctccgtat ttcacaccgg ggtgagcaaa agagcctatt ccgtcgtttt
     3181 cgtaaatcca ctcgtaaagt tggaacagat cgctttcgtc gcggtgggtg aaatgctggg
     3241 tgtcgtagac ggtaatatga ccctggccgg tcaaggtcca ctcgaacccg gcgattgcca
     3301 ggaaccgtcc atcctcgttc atttctctgg cagcttgaaa ggttttcggt aaccgcatcg
     3361 acccgtcagc cagaggatac tgcagatagt gacaatgatc ggtaacgcct tggatatcca
     3421 gctttccgta atctcttgcg tgcgtatagg cctcccaagg gtctccggct ccatccgagt
     3481 aagaggtgtg cgcatgcaaa tttccgtaaa aaatatggag ctgcgcgaag gcgctaaagg
     3541 agaaaaacaa cccggccaaa gcacacaata agacgatttt caaaacgcgg cgcatagttc
     3601 ctcctaaaaa ctaaagggcg cgtcgaaacg cgccccgtga tgtgtgagtt tagtactcgt
     3661 cgagaacctt ttgagccttt tcggtcgcag tttttaatac gttttcgacg ttggcgttca
     3721 tatcggtcat aatcgtctgc caactggaaa gcataatatt gaagatttcg cgatatggcc
     3781 cgatataagg tctccgaacg gcgctttcct tcaactgcag ataggtgacc tccgccctcg
     3841 ggtctttctt gaacaactct tggatttcgg gagattcgta ggctgataca cggctagcca
     3901 tataccctgt ggctagcgac cattttttcg tgttcggtgt cgcggtgaca aacttcatga
     3961 actcccaagc tgcctgtttt tcgttttgcg gtttgttctt gaacatatag atgttcgcgc
     4021 caccgatcgg tgtggccttt ttcacttgat aaggcataaa ggtagcgcct aagtcgaact
     4081 ttgcgttctg aatcatgtag gcgagacttc ctgtcgagcg gcaaaccata gcgaccttac
     4141 ccgagaggtc catcgcgttc ccttgttccg gtatggtata aacgaaaacg ccttctcgta
     4201 accctttttg gaaaaatttc caggcgtcta tcgtccccgg agaatcgagt aaaaccttca
     4261 tcccgtcttc ggaaacgatg tctccgccaa actgccaggc gtaggattcg agtatccagt
     4321 catcgtcacc cagccgatag ccataataat cgggcccgag tttctttaca gccttcgcgg
     4381 cttcgtagac ttcttcccaa gttctgggtg gtttattagg atccaaaccc gcagctcgga
     4441 aaacgtcgcg gttgatgtac aataatgggg tgctcgtgtt caacggaaag ccgtatagct
     4501 taccgtagaa ggtgttggct gcgaggaatc tggggaagaa atcagacaag gtagccttga
     4561 attcggggtc tttgtttatg aactcatcca agttctggaa agcatccccg tccaagaact
     4621 ggcctattcg gctttgttcg atctgggcga tatgcggcag gttgcccgcc accatagacg
     4681 ccacgagctt ttgcatcgtg tcctcgtaac tacccgaata gactccttct accacgattt
     4741 ttccttcgtt gagtttgtta aactcatcaa ttaactctaa gagcgttggg gaataaatcc
     4801 cagtttgcgc ataccagacg acgaccttca ccggtgcagc gaagagcatc atcgcgcaaa
     4861 tcaagagcgt taccgtgatc catactttct tcatgttctc tttcctcctt ttttcattct
     4921 ttgatgccag aaacgctaat accttcgaca aactgttttt gcacaaagaa aaagaaaatg
     4981 atgatcggca ccactacaag agtcgaggcg gccattcgat aggtccagcg ggtttcccaa
     5041 ccgctcacga attgcgagat gccgacctga atcatctgat acttgggatc gctgacggaa
     5101 accagcggcc acatatactc gttccaatgg gcgatgaagg tgaagagcga catcgtccaa
     5161 agcgccggct tcgtgagcgg gagaatgatg gtgagaaaaa tcttcagatg gccggctccg
     5221 tcgatgatgg cggcctcttc cagctctctt gggattccca ggaagaactg tcgtagaagg
     5281 aaaatcccga aagcgctaga cataaatggg atgatcaaca cgcggtaact gtcgatcatc
     5341 cccaggtttt tcacaatgat gtagagcggt actaaggtca cctccggcgg aaccatcagt
     5401 gtcccgacca agattaggaa gatgaaatcc cttcctttga accgcaaacg agaaagccca
     5461 taggcggcga agctgttaaa cagaagttga agtaaggtga cagacagggc gacgaaaagg
     5521 gaattgaaaa ggtatctccc catagggatc tgggccatcg caccggcgaa atttcgaaaa
     5581 tcaaagaaat ttgggtaaaa tttcgggggc aaggtgaaca cttcgctttc gtctttgaaa
     5641 gcggtcacga gcatccagat gaacggcaga ttcacgagca gcataatgac gataattgca
     5701 atatacaggg gtatacgtat ccagagtgag ggcgcctttc tcatgcgtaa tgcaccctcc
     5761 ttttactgaa tcggaactgg atcaacgtaa agactaccat catgaggaac atcaccacgg
     5821 agatggcggc ggcgtaacca aacttcaagt atgcgaatgc attttcgtac aggtaaaaag
     5881 tgatgacccg cgtactatta gcagggcctc cgcgagttat tacgctaacg gaaacaaaca
     5941 cacggaagga agcgatgaac tgcataacgg taacgaagag cgtagtcgga gatagaagcg
     6001 gccaagtgat cgacgtaaac ttactaaaag tggatgcgcc atcgatgtca gccgcttcgt
     6061 aataggttct cgggatgacc tggagtccgg ctaaaaagag gaccatatta taaccgatcc
     6121 ttttccatat gcctatgatc atcagcgcga tcaaagcgta gtccggatca ctcaaccagt
     6181 tgatcgactt agcgccaaac caactgagga aataattcag cagccctacg ttcccctcga
     6241 atatccaagc ccagatcacc gccgcggcag ccatcgaggt gatgacgggg atatagaaga
     6301 aggttcgaaa gacccctttt cctttaagcc aagggagatt gaccagcaag gccaggagta
     6361 acccgatgag tatggaaacg gggacggtta ccgccacata gatgaaggtc actgacatcg
     6421 agctccagaa atctgaagag gtaaacattg aggtatagtt atcgaaaccg ataaaccgca
     6481 tcgggcggat gagattccat ctaaaaaaac tgacgaaaaa tgaccatccc aaagggaagt
     6541 agatgaacag tatctgtgtg accaatgcag gcgacacgaa gacccatccg gtcagcgctt
     6601 ctctcgtctt tcttctcatg tatggcacct acccttatcg tttcgcattt tgggacttgc
     6661 ttctgatgaa gagggcaaaa tcccgtccct cgaatgcccg cagggaatta tcctggaatc
     6721 acccaagcgt ttttttggga gaagaaaagg cgtttttctt cgcctacccg gggtaaagac
     6781 ccttccgaga gattgtattc caaagcgttg atgacagaac cgtcgcgcag ttctaccgag
     6841 tattcgaggt gagaacccaa gaatatgaac gaacgcacta tgccagaaat ggaattaaaa
     6901 ccctcgttgg aactgggagt cagggagaac gcctccggac gaacaacgat ccgggctcct
     6961 tgaccttcgg ctactaaaag gttccggcac ttgaaggatt ggtttagagt cttcgcgtgg
     7021 acaatacagg tctcgccgtc tctactatcc acgacgccgt cgataaaatt gactttaccg
     7081 ataaaatctg ccacaaacgc attagccggg cgcgtgtaaa cctcgaacgg actccctttt
     7141 tgcataatct tgcctttgtc cataatcacg atcaggtcgg aaatgctcat cgcttccact
     7201 tggtcatgag tcacgtatac gcttgtaatc ttcatctcct gttggatacg tcgtatctcc
     7261 aagcgcatcg actcccggag cttggcatcc aaattcgaaa gcggctcatc gaatagcaga
     7321 accttcggtt tcatcaccaa gcttctggcc aaggcaaccc gctgttgctg cccaccggac
     7381 aactggtccg gtctcctttt ttccaatccg cgcaaaccaa ccaaatcgat catatcgcgg
     7441 gcctctttac gcacgacatc ccgggaaagg cgcttgatct tcagcccgaa agagacgttc
     7501 tcttccacgc tcaggtgtgg aaagagggcg tagctttgaa acaccatcga aatattccgt
     7561 tgattcggcg gtagaaaggt aatatcctct ccttccagtc tgacgatgcc atctgtcgga
     7621 agctcgaatc cggcgatcat ccgaagggtc gtggtttttc cgcagccgga ggggccgagc
     7681 aaagtgatca aggcccccga cgggatttcc aaccatatcc ggtcgaccgc ccttaattca
     7741 gcgccgtcgc ttctgaaggt tttgacgagg ttttccaaaa cgagacccat aagctcacct
     7801 aaccccgtag ttttggttcg ataggctgat tctttgacgt aaaaagagct tgagagacaa
     7861 cataaagatc aaaataatgg aaatcaagat cacggaatag gcgcatgctt cggaaagccg
     7921 gcctgagtct gtttgactca gtatctgcac cgtcatcagg ttccatttcg ctgaaacgag
     7981 aaagatcgcg gcgctgatcg ccgtcatcgc ccgtacgaaa gaaaagacga gggcggaaaa
     8041 gaaggccggg acgatcagcg gcaacgtgat cctccgaaat acggcgcggt tgttcgcgcc
     8101 taaggtgaag gccgcttctt ctatcgcggg atctacttgg ttgagaaggg cgatccccga
     8161 ttgtatcccg acaggaatat accgaaacac gaaattgagc accaggatcg ccagggtacc
     8221 cgtgagtaaa aacggggggg tgttgaaagc gaagatgtat ccgataccaa ttacggttcc
     8281 gggtaccgcg aagctgagca gagagaggaa gtccatcgcc ccttttcctg ggaatcgttt
     8341 gcgtacgatt aaaaaggcga taatcattcc caaaagccca gacagtgggg ttgaggagaa
     8401 ggcgatgacc agagtgtcct taatcgactc taatcccaca tcgagcacat acttgaagtt
     8461 atctagagtg aagcgactac tcatccccca agcctgcgtg aaggatcccc aaacgatcgt
     8521 cgcgtaaatg agtagaatga agagcgaaat tatcaggcaa agcgagaaca gagcccattt
     8581 gatagggcgg ttcacgctct tgatgaccga agtggtcggt tttccgctca aggtgacgta
     8641 ccgctttctg ctgacccagt acttttgcgc taagaacgcg aggatcgaag gcaagaggag
     8701 ccagacggct aaagccgcgc cagttctgag gttgtacatc ccggttatct ccaagtacgc
     8761 ctgaacggac aaaacgggga aacgactacc ggcgaggatc aaggggttcc cgaagtctgc
     8821 gagagactct ataaacaaaa ccagcaaggt actggcgatc ccggggattg aaacgggcaa
     8881 gatgaccttt cggaaggctt cccatctgct cgctccgaga ttgaaagccg catcttcgag
     8941 tgtgggtgag atagtcgaaa tcaccccgtc cagggtgatg aaggcaacgg gaaagaagca
     9001 gaccatctga gccatcatca ggccccatag gccgtacacc gggaagttgc gtatgccgaa
     9061 aacttttgcc gtgattaaac cgttaaaccc aaacagcata atcagtgcca tcgctccgat
     9121 gaagggtggg aaaacgaccg gcatcaacgc gacgaatcgg aaaaaccttt tgagggggat
     9181 attcgtgcgg ttgagagtat aggcgaaaag gtatccgatc gccacgccga aaatagcagt
     9241 gagggaggcg actaaaaggc tgttatagaa tccttggcgc aaatagttgt tggagagggt
     9301 ctgacggtaa acgtccaagc tcaatgttcc gtcacctttc tcgaagctaa ccatcgctac
     9361 ctttatcaac ggataaaggg aaaacaggaa cagcaagaaa aaaatcccga tgatcaacaa
     9421 taacagaaaa ggttctttcg ttaaggcttt cagagagcga aaaagctcct tcgacttcaa
     9481 cggtacaccc ccggcgcgcg tgtgcgctct gaaaaagacc ggcttccggt tcacccggaa
     9541 gccttgttct atttaacctc gcctgagtta ttggacaggc aacacctctt ttacccaacg
     9601 ctccaggagg cggaccttct cttgcgctgc ccaaatgccg tcaacggtta tgagcttaat
     9661 cgtggaaaga ggttggagcg agccgaaatc gacgtcgttg cgtatggggt aaaaataggt
     9721 tttctgttct acgatttgct gttgcccgtt tttcgagatg agccaatcga tcagtttttg
     9781 cgcttctccg atgtttttag cgccctttaa aatcgaagcg catgcgcatt catagggaac
     9841 gccctcttcc ggaaagatga tttcgacagg atagccttgt tctttgaact ggaaaaaggc
     9901 gggtgtgaac tgcaaaccca tcaacgcctg accggtgcca accgcttttg acggacccgt
     9961 accgctttgg gtataggttt gcacgttcgg agccattttt ttcaggaatt cgatcatccc
    10021 gtcttctcca taaagcataa tcaagccggt catcatcgag taggctgtcc cggacgcttg
    10081 cgggttcggg tattggatca tcttgcggta agccacgtgg aagaggtctt cccaggtctt
    10141 cggcatcggc gcttttagct gcgcgagcac ctgggtgttg atcccgatcc ctaaggggtt
    10201 catatagaag gcgtggaaaa acccgtccac atcatagaac tccgggctaa tcccgtaaac
    10261 gtgcaaagat ttgtaggcca tcgtcagccc tttttctttc gcgagaatgt ggttttccat
    10321 cggcgctcca aaccaaacat ccgcttgggg gttattcttt tccgcttcaa ttctagccat
    10381 cgccggaccg gaggaaagga aaacgaagtt cagttttatc cccgtttccg ctgaaaaggc
    10441 gttgagaatc ttccgcgcgt tggcctcatc gacgctggag tagatgacga gttcggagaa
    10501 accaatcaat gcgatgagaa ggagagagag aaagaatacg cacttcttca tactaacacc
    10561 tccaaagttt tcttaaagat gtgggtctat gacataccca attttaccat agttcgcagg
    10621 cagttcccta ttccgtgtgg tatcaaatcg gggtgtgata ggaaaaaatg acaaaaacga
    10681 ggagggaaaa aaaggccccc gaaggagcca ccataacttc gcacacgctg cccttcaaat
    10741 cgcgttcaag ccctttgccg gagtctctac attaaatgct tttttaattc ttcctcgttt
    10801 ttcatatcga tcaactcgag tcccaaggag tcaagttgct ctattgtaag ggacttcagt
    10861 ctttcaaaat acttttgcga ggccttaggg aattttttgg ctatttgttt ccaaaggagc
    10921 tcttcccggc cctcttctct gccttttttt attccttcct gtctgagcat atccgcgatt
    10981 gtttgcatct tttcgctcct ttcttccgat accgttttca tcagttctga caattgttgg
    11041 aaatactccc tttccattgt atcgaacagg tagattatac agacctggaa aaaccgttca
    11101 ttcgcttctg tctgcggcaa ttccgaaatc gtcactgtga ctcggagcat cacttctcgt
    11161 atcgcttctt ttccctcgag cgctctcatt cttatgacgt ccaaatagat acgaagacgt
    11221 ttcgcccctt tgacctcttc ttctctcaat gacgaaattt caaacagttc atatttgtag
    11281 tccgggatgt agtctttcaa gtccccgtcc atactgtcgc tgtttgaaaa gcgattcgaa
    11341 aaccattgcg gcgcttccca tttctccctc ccctggtaga aaaccacggg tagaatcact
    11401 ggtagaggac gcggtactgt cttcttccat atggaggtca tataccccag gagttgtagg
    11461 ctcacgtgtt tgtcaacctt gcttttgtgt tcgaacagca gatagacata cgcctcttcc
    11521 tgttgcatct tcacccgaaa gagcagatcg aagaagtatt ccgagaggtc tttctcgatg
    11581 taactgtctg tttctatcgt tatcgatt
//
LOCUS       DAOPXN010000065        11511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5722_length_11511_cov_5.358764, whole genome shotgun sequence.
ACCESSION   DAOPXN010000065 DAOPXN010000000
VERSION     DAOPXN010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5722_length_11511_cov_5.358764"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PKM49_05135"
     CDS             complement(<1..436)
                     /locus_tag="PKM49_05135"
                     /inference="COORDINATES: protein motif:HMM:NF021034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HNY82231.1"
                     /translation="MNGVNDQIEGFVERLWGLRKSEISLLKRNAGCSLPESLNTLPIF
                     VRICPPGLYKIQEELYHLVSTLYGLNPYPEEGDFGATMGKVSKKTQSKSVERRFAILL
                     DSKIGVSSVSGLEMGELRFRLRQNVKLAASKEVGVNWYQLTKD"
     gene            complement(433..2001)
                     /gene="casA"
                     /locus_tag="PKM49_05140"
     CDS             complement(433..2001)
                     /gene="casA"
                     /locus_tag="PKM49_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HNY82232.1"
                     /translation="MTVSFDVWKEPWIPVLNTKGTIEEKGIRDCLCSSHELEAIVDPS
                     PLIQFGLYRLLIVMLMDIYDSLTANRPYDFILDLLEEGRFCPDKIDDYYNNRVGPSRF
                     DLFDETYPFLQSAFNEEYDAEDKPVSYLLHHQPSGTNVIHFHHVFEGDLALSPVVCAR
                     ALCTFQPFITTGGRGNAPSINGVPPLYVLVKGRSLFETLCLNTVWGQPDERFSGAEPV
                     SWRSLKEVVPGQERDTATYLEGLTWRPRRVRLIPSDGGVCTYSNQHSSTLVRRMNFSA
                     GFTARLKGAWVDPNAASIITNKDLRMMTPKEDREIWRDVGPLLLLHENDYSGKREKIR
                     YEKPLVLRQYEQLKRELPDMLKTEVYGLRTDSKNKYFEWVYEPLHIPMAYDADGSKRG
                     LVQKVMETTDQVCNWLGIALKKLAPASSEARSYIITAQRMFWDIAREPFFELLDAMSG
                     FLDDVNIEKMMMREWKKRLSAICNRALQEALQEFSETGRDLMRQAEARQVFWGNFQRL
                     FKVYSENEQKGVTV"
     gene            complement(1998..4691)
                     /gene="cas3"
                     /locus_tag="PKM49_05145"
     CDS             complement(1998..4691)
                     /gene="cas3"
                     /locus_tag="PKM49_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HNY82233.1"
                     /translation="MENDLIVSCLKRLWAKTNPFYPLFQHLIDVGVGVGFLLRHSSYR
                     CLGEKLARSFGAPLDQSVPWCQYLCAMHDIGKCHPLFQGKADTDFSRDLKAMGKIPAI
                     VTPGFRHEAFSDDWVYRLLKETFDWKRGQARTMGSILHGHHGNYFSKVVPEPGSLSAF
                     WEEVRQEFAHMLLNVFDPPELKGHTIAHHSNTGVILSGLCVLSDWVVSNSEVFEMGVF
                     DGFAVDYAPISEKRVSDAFRKIGFTESINWPRRPLFRQVWSNLGIRELRPIQENVESL
                     VQDDIEPGLVIIEAPMGEGKTEAALHLAAHWIGRNDLSGLYIGLPTAATSNQMYQRVK
                     SLLSQHLSNSRLPIKLIHGRAWLIDELTPHKEIETDQENGYSVHEWFSPKKRSLLSPY
                     GVGTVDQAMFSALNVKFGFLRQLSLSGKAFVIDEVHAYDAYMSQIIERLLAWCASLET
                     PVILLSATLPSAKKRAFIQAYRSGKNFPDQHWEVSSEQHYPLLSVSSLTGEYMEYPVQ
                     GAAKTQTIEIAHHPFLNDTDSIADLISPKISKVGGVYCCIVNTVRNAQQLYKSLRQRM
                     PETELLLYHARFLAGRRQQIEEEALRRYDKRSLLPVDDPERQERPKTSLLIATQVVEQ
                     SLDVDFDEMFTEIAPIDLLLQRAGRLHRHSGRTRPTGKSPTLHIFGPKENEWDWGSTG
                     KVYEPFILAKTRWLLQGKTQLKLPEEIRCLVESVYNDEKDIPDSFIAPSKIDQFYDRM
                     KESQAQEENEARQYLLPKPNKESYMIVDRASHAYEEEEEGMGDFFRASTRMGNNTCTL
                     LVLRDEDASGPDFRNTDKKRLKELMLSTTSVPRWWLRDVEPDAGYAKIEEARNFPVKA
                     LVLRTDDGGRWKGKGLANSGVTITNDRELGITYEKTEVKSD"
     gene            5674..6921
                     /locus_tag="PKM49_05150"
     CDS             5674..6921
                     /locus_tag="PKM49_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine hydrolase"
                     /protein_id="HNY82234.1"
                     /translation="MQRLFASDLHVGDGLFNDNFSLDEEFSSLLESVASSGKPTELVL
                     LGDIFELLESKMVRDMGLCSFEEVCEGIDVGVVDQIFSRHPRLILSLKKFMRTNRIVY
                     VVGNHDYYFLKREDLCDRVNEYLAEGEGISFVPYFYDRHWGLFGYHGSNFDLTNRFGK
                     EKKTGRLIPPIGDYMARYMMIHFRERLRDERVPSSIVDDFDDVRPNIDVFDWVKYIKE
                     TFHLSINLAELWMTELLKMLQTVSAQSWMKMNYPRAAKLSGLFVNNKGGVRLGRIMIG
                     IVAKLRQLRRTDYMRRKAKKALVGAVHKEPDRFQESDFWGFCDLPDIDYRNLSGVLFG
                     HRHRFDSVIHTVNGENRFYINTGTWRNVIERGRKRDQEKFVKRTEYSYALIHDDGGEL
                     TIQTIMNSKIRNKALAIPRAGVV"
     gene            6922..9726
                     /gene="polA"
                     /locus_tag="PKM49_05155"
     CDS             6922..9726
                     /gene="polA"
                     /locus_tag="PKM49_05155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNY82235.1"
                     /translation="MRKLYLIDGTGLVYKAYYGIKELNTSKGQPVNALYGLARMLTKL
                     SRERLLEGQYVTFFMDAGRTTFRTELLDEYKAQRPPMPETLREQMKEVAPLAEGFGIP
                     VLSMEGFEADDLIATYVRKYRDLCDEIWILTSDKDLCQLIDDKVKMLRAEKGVADLVV
                     YDREKVLEKYGVTPAQMIDYLSIVGDKTDNIPGIRGIGEKGALQLLTEYPTLDAIYEH
                     LDSVPEKLRGKLTEGRADAFLSQQLVRLKADIPISQDFEDLRYHSVNPTQLLQDFRRF
                     EFRSLEREWMPTLFGAGGDGTSEREQEAIPTSSACQEASPAPTEEDRDYRVVVDEIGF
                     RQLLEAMHKVEDCAFDTETDSLNTRDARLVGLSISMAERAGFYLPVAHVEGPNLSEPL
                     VAEFFKALKEKRVIGHNLKYDFEVVAHNGYPLPERYFDTMIAAYLLDPDRSRFHLDGL
                     ALEVSSQAPLSYKDLMSAHPFARDFSQIPIEQAARYSVEDADLSLRLCRRFEPELKEN
                     GLDPLFERIEMPLLRVLVEMETTGVYFDREALLDLSERMGAQIKGLEKEVVSLAGEPF
                     NLDSPIQLGKVLFEKLGLPSKKSTAKSKRYSTDKSVLEELAGEYEIAEKILEYRKLNK
                     LKTTYIDAIPNLIHPKTQRVHTSFNQAGTATGRLSSSEPNLQNLPVKNEEGKEIRAAI
                     KPQRPGWLLLSADYSQIELRVMAFLSQDTHLLEAFRRSQDIHRYTASRLFDIPWSEVD
                     ENQRRIGKMINFSIIYGVSSFGLSSRLGIGRKDAEKFIDAYFRAYPGIKDYMNAAIAL
                     ANSDGIVRTLYGRLRRIPFIAARNPGLRKEAERVAINSPVQGTAADVIKIAMIRIYER
                     LKREGLQARLILQVHDELVFELPESEKASLQAIVAEEMEQVLVGEPHSAYGAHAGGMK
                     GIPLLNSISLKVDIEVSEHY"
     gene            9740..10669
                     /gene="ftcD"
                     /locus_tag="PKM49_05160"
     CDS             9740..10669
                     /gene="ftcD"
                     /locus_tag="PKM49_05160"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HNY82236.1"
                     /translation="MKWIETVPNFSEGRRKEVVDAIVAQAKAYDHVWILDESLDYDHN
                     RSVVTMVGEPESVLKALFDMTKKASELIDLRVHRGEHPRMGATDVIPLIPLRNVTVEE
                     CVTHAATLGKRIAEELEIPVYLYEKSATRDERKDLSNIRQGQFEGFAAKIQKPEWCPD
                     FGRREIHPSAGACAVGVREFLIAFNINLRTQDLEIANKIAKAIRNISGGFRYVKALGM
                     ELKEKQMVQVSINMTNYKRSTLFRVYEMVKLEAERYAVAIDSSEIVGLTPLQALIDVA
                     SFYLRLDGLTPENVLEMKMLEILMKDADGQKTS"
BASE COUNT         2801 a         2896 c         3089 g         2725 t
ORIGIN      
        1 aatctttcgt aagctggtac caattgacac ccacttcttt actcgcggcc aattttacgt
       61 tttgcctcaa gcgaaaacgg agttcgccca tttcgagtcc gctcacggag gaaacaccga
      121 ttttgctgtc gaggagtatg gcgaatcgcc tttcgacact tttggattgg gtttttttcg
      181 acacttttcc catcgtcgcg ccgaaatccc cttcttcggg gtacggattc aacccgtaca
      241 gagtgctcac cagatggtaa agctcttctt gtattttata caagcctggt gggcaaattc
      301 gaacgaagat gggtagcgtg ttgagagact ccggcagaga acaccccgcg tttcttttca
      361 gcaggctgat ttcgctttta cgcaaccccc acaacctctc gacgaagcct tctatttggt
      421 cgtttactcc attcatacgg tcaccccctt ttgttcgttc tctgaataaa ccttaaaaag
      481 ccgttggaaa ttgccccaaa aaacctggcg cgcttccgct tgacgcatca aatcccttcc
      541 ggtttcggaa aactcctgta acgcctcttg cagcgccctg ttgcagatgg cggacaggcg
      601 ctttttccac tctcgcatca tcatcttctc gatattcacg tcatccaaaa agccgctcat
      661 agcgtccaat agttcgaaaa agggttctct cgcgatatcc caaaacatcc gttgagcagt
      721 aatgatgtaa gaacgcgcct cgctagaagc gggagcgagt ttctttagcg cgattcctag
      781 ccaattacac acctgatccg tagtttccat cactttttgt accaaaccgc gcttgctccc
      841 gtccgcgtcg taagccatag gaatgtgtaa tggctcatac acccactcga agtacttgtt
      901 cttggagtct gttcgaaggc cgtagacttc cgttttgagc atatcgggga gttctctttt
      961 tagttgctcg tattgccgta gaaccaaagg tttttcgtaa cggatctttt ccctttttcc
     1021 cgagtagtcg ttttcgtgta gaaggagcag aggaccgaca tctctccaaa tctcccggtc
     1081 ctctttaggc gtcatcatgc ggagatcctt attggtgata atcgaagccg cgttgggatc
     1141 gacccaagca cctttcaatc gcgccgtgaa tccagcggaa aagttcattc tgcgcaccaa
     1201 tgtgctgctg tgttgatttg aatacgtgca gaccccaccg tcagaaggga tgagccgcac
     1261 cctgcgcggc ctccaggtga ggccttccag gtaggtcgcc gtgtcccttt cttgccccgg
     1321 taccacctct ttgagtgaac gccaagatac cggttctgct cccgagaatc gctcatccgg
     1381 ttgcccccag accgtgttca agcagagcgt ttcgaaaagg gatcgcccct ttactagcac
     1441 ataaaggggc ggaactccgt tgatactcgg cgcgttccct cttccgcctg tcgtaatgaa
     1501 gggttggaac gtacacagcg cccgagcgca gacgacggga gacaacgcca ggtcaccctc
     1561 gaaaacgtgg tggaaatgga tcacgttcgt cccgcttggc tggtggtgca ataggtaaga
     1621 cacaggtttg tcttctgcgt catactcttc gttgaacgcg ctttgtagga agggatatgt
     1681 ttcgtcgaac aaatcgaacc ggctcggccc cacccgatta ttgtagtaat catcgatttt
     1741 atccgggcag aaccgccctt cttctagcaa atcgaggatg aaatcgtaag gccggttcgc
     1801 cgtaagcgaa tcgtagatat ccatcagcat tacgatcagc aggcgataga gaccgaactg
     1861 aatcaggggg gaagggtcta cgatggcttc cagttcgtgc gatgaacaca gacagtctcg
     1921 aatccccttt tcttcgattg tcccttttgt gttcaaaaca ggtatccacg gttctttcca
     1981 aacgtcaaaa gatacggtca atcgcttttc acctccgtct tttcgtatgt gattcccaat
     2041 tctcgatcat tcgttatggt caccccgctg tttgccaacc cttttccttt ccaacgtcca
     2101 ccgtcgtccg ttctcaatac gagcgctttc acggggaagt tccgcgcctc ctcaattttg
     2161 gcatatcccg catcgggttc tacatctcgc agccaccatc ggggcacgct ggtcgtcgag
     2221 agcatcagtt ctttcagtcg ttttttgtcc gtatttctga aatcgggacc gcttgcgtct
     2281 tcgtcccgta ggaccaataa cgtgcacgta ttgttgccca tccgcgtact tgccctgaaa
     2341 aagtcgccca taccttcctc ctcttcttcg taggcgtggc tggctctatc cacgatcata
     2401 tacgattctt tgttgggttt cgggagcaag tattgtctag cttcattttc ttcctgcgct
     2461 tgcgattcct tcattctatc gtaaaattgg tctatcttcg acggcgcgat gaaagaatcc
     2521 gggatgtctt tttcgtcgtt gtagaccgat tcgacgagac accggatttc ttcggggagc
     2581 tttaattgcg ttttgccttg caaaagccat cgcgttttcg ccaagatgaa cggctcgtag
     2641 actttgcccg tcgatcccca gtcccattcg ttttcctttg ggccgaaaat atggagcgtg
     2701 ggggatttcc ctgttgggcg cgttctacct gaatgtcggt gcaacctccc tgctctttgt
     2761 aataacaagt cgatcggggc tatttctgtg aacatttcgt caaaatcgac gtcaaggctt
     2821 tgctccacga cttgagtcgc tatgagcaga ctggttttgg ggcgttcttg gcgctccgga
     2881 tcgtctaccg ggagaaggct gcgtttgtcg tatcttctca gcgcttcttc ttctatctgc
     2941 tgtcgccgcc ccgccaagaa ccgagcgtga taaaggagga gctctgtttc gggcattcgc
     3001 tgccgtaagg acttatagag ttgctgcgcg tttctcaccg tattgacgat gcagcaatat
     3061 accccgccaa ctttggatat ttttggggaa atgagatcgg ctatcgagtc cgtatcgttt
     3121 aagaagggat ggtgggctat ttctatcgtc tgagttttcg cggcgccctg aacggggtac
     3181 tccatatatt cgcctgtcag agaagacacg gacagcaatg ggtagtgttg ttctgaagaa
     3241 acttcccaat gctggtcggg aaagtttttg ccggaacggt aggcttgaat gaacgctcgc
     3301 ttctttgccg agggaagcgt ggcggagagc aagatcacgg gagtttcaag ggaagcgcac
     3361 caggcaagca accgctcgat aatttgcgac atatacgcgt cgtaagcgtg aacttcatct
     3421 atgacaaacg ccttcccgga aaggctgagt tgccgaagaa acccgaactt gacgtttagc
     3481 gcgctgaaca tcgcttgatc gactgtgcct acaccgtaag gagagagcaa actccgcttt
     3541 ttcggcgaaa accactcgtg aacagaatag ccgttttcct gatcagtttc tatctctttg
     3601 tgcggtgtca attcgtcgat caaccacgct cttccgtgaa tgagtttgat gggaaggcga
     3661 gaattggaaa ggtgttgaga caagagcgac ttgacccgct gatacatttg attgctcgtt
     3721 gcggctgtcg ggagtccgat atataacccg gacaaatcat tccgacctat ccagtgagcg
     3781 gctaagtgga gggccgcttc tgttttccct tctcccatcg gcgcttcgat gatcacgagc
     3841 ccgggttcga tgtcgtcctg aacgagggat tcgacatttt cctgtatggg gcgtagttca
     3901 cgaattccca agttgctcca cacttgtcga aagagggggc gacgcggcca attgatactt
     3961 tcggtgaacc cgatttttcg gaaggcatcg gatactctct tctcgctgat aggggcgtaa
     4021 tccactgcga atccatcaaa gacccccatt tcgaatacct cggaatttga cacaacccag
     4081 tcagataaaa cgcataaacc ggaaaggata actcccgtat tgctgtgatg cgcgatcgtg
     4141 tgccctttca attctggcgg gtcgaacacg tttagaagca tatgggcaaa ttcctgtcgt
     4201 acttcttccc aaaacgcgct taagcttcca ggctccggta ccactttgga gaaataattg
     4261 ccgtgatgcc cgtggagtat tgaacccatc gtccgagcct gccccctctt ccaatcgaac
     4321 gtttctttaa gcagccgata aacccagtca tccgagaacg cttcgtggcg aaagcccggt
     4381 gttacgatgg cagggatttt ccccatcgct ttcaggtcgc gcgaaaaatc agtgtccgct
     4441 tttccttgga acaacggatg acatttgccg atatcgtgca tagcgcagag gtattggcac
     4501 cagggaacgg attgatcgag aggggcgccg aaagatcgcg caagcttctc ccccaggcag
     4561 cgatacgaag aatgacgtaa aagaaacccg acgccgaccc caacatcaat gaggtgttgg
     4621 aataacgggt aaaaaggatt cgttttcgcc cataaacgct tcaggcagct aactattaga
     4681 tcgttttcca taggagttca gcctcaggat ttcatcttcc atttctatga tttcaccttg
     4741 accgaatcac gtaactgatt ctcgtcaaaa taaagacaga ttcgaagaga ttctttctca
     4801 ccgtaggccc ccactaaaac atatcgatga aaaaagcata gaaatcggat tgtgaaaaga
     4861 agaaaaaagc aaaaatagat gtctctgcac atcatttcat attgtcaagt cacaacgaaa
     4921 acgacacaga gaagggagtg taatgtttag tagattttac taccgatcgg acatattgtc
     4981 aaggataggt taggaacata tttcgttacg gtggtcgttc gctatggggt atttcgctcg
     5041 tacgcgtctg aggtacgtat caaaaatcga gggcgcgggc gacccttgtt cggcggcgct
     5101 gccacggttg tgcgttgcga gtaaaaacgc gtaccggata agaatggcgt tggggcgcct
     5161 aggaagaacc cgggacacaa tgggacaccc cttgggggcg ctctgagaac gtctgggaac
     5221 gccgaatttt tcgttcgaca ggtatttcaa aaacaaaaaa aattcgcact gaggacattg
     5281 agaacacaaa gggaaaatag gcgatgggtt gggaacaact gggattgccc cactgggaac
     5341 gccgaacttc agttcggcat ttttttcaaa aaccaaaaaa agtttcgcac ggagaacaca
     5401 aagagaaaat cgacaactgt tcggatgcac aggctctcgc cctgagaagc cataagcgac
     5461 tacgccgcga tgttcgtgcg gttcatcgcg gggtaagtgg tttttacgac cccgcgatgc
     5521 gcacccataa aagcagatcg cggggtcccg ttggttaccc cgcgatgaac ctctatttta
     5581 tgtcaccccg taacttgacc cactcctccc agtttgaacg ctatggttag tggtataata
     5641 aggcggagaa acgagaaaat agggggaaaa cggatgcaac ggttattcgc cagcgacttg
     5701 cacgtgggcg acggtctgtt taacgacaat tttagcttgg acgaagagtt ttcttccttg
     5761 ctggaatcgg ttgcttcatc agggaaaccg acggagctcg tgcttttggg cgatatcttc
     5821 gaacttctag aaagtaaaat ggtccgcgat atgggcctat gctccttcga agaggtatgt
     5881 gagggtatcg acgtcggggt ggtcgatcag attttctccc gtcatccgag gttgatctta
     5941 agcctaaaaa agtttatgcg gacgaaccgt atcgtctacg tcgtcggcaa ccatgattat
     6001 tacttcctca aacgcgagga cctctgcgat cgcgtgaacg agtatctggc cgaaggagag
     6061 ggcatctcgt tcgttcccta tttctacgat cggcattggg ggctttttgg ctaccacggc
     6121 agtaacttcg acttgacgaa ccgttttgga aaggaaaaaa agaccggacg tttgatccca
     6181 cccatcggtg actacatggc ccggtatatg atgatacact ttcgcgagcg gctccgggac
     6241 gagcgggtgc cctcttccat cgtcgatgac ttcgacgacg tccgcccgaa tatcgatgtg
     6301 ttcgattggg tgaagtacat caaagaaacc ttccatctca gtatcaacct cgcggagctt
     6361 tggatgaccg aattgctcaa aatgctacag acggtgtcgg cgcaaagctg gatgaaaatg
     6421 aactacccga gggcggcgaa actgtccggg cttttcgtga acaacaaggg aggggttcgg
     6481 ttggggcgga ttatgatcgg gatcgtcgcg aagctgcgtc aacttcgccg aaccgactat
     6541 atgcggcgaa aagcgaaaaa ggctctcgtg ggcgccgttc ataaggagcc cgatcgtttt
     6601 caggaatctg atttttgggg gttttgcgat ctccccgaca tcgattaccg gaatctgtcc
     6661 ggcgttttat ttggccatcg tcaccgcttc gacagcgtca tccacacggt gaacggtgaa
     6721 aatcggttct atatcaacac tggcacgtgg cggaacgtaa tcgagagggg aaggaaacga
     6781 gatcaagaaa aattcgtgaa gcgaacggag tactcctacg ccttgatcca cgacgacgga
     6841 ggagaattga ccatccaaac gattatgaac agtaaaatac gtaataaagc cctggcgata
     6901 cctcgggcgg gagtggtgta gatgcgaaag ctgtacctga tagacggaac cggcttggtg
     6961 tacaaagcct attacggcat caaggagctg aacacctcca aaggccaacc ggtcaacgcg
     7021 ctttacggcc tcgcgcgaat gctgacgaag ttgtccagag agcgcctcct cgaggggcaa
     7081 tacgtcacct tctttatgga tgcggggcgg accaccttca gaacggagtt gctggacgaa
     7141 tataaggcgc agcgcccgcc tatgccggag actttgaggg aacagatgaa ggaagtggct
     7201 ccattggcgg aaggattcgg gattccggtc ctatcgatgg aaggcttcga agccgacgac
     7261 ctgatcgcca cctacgtgag gaagtaccgg gacctatgtg atgaaatatg gatcctcacc
     7321 agcgataaag acctttgcca gctcatcgac gataaagtga aaatgctgcg cgcagaaaag
     7381 ggagtcgccg acttggttgt ctatgatcgc gagaaggttc tagagaagta cggcgtgact
     7441 ccggcgcaga tgatcgacta cctatcgatc gtcggcgaca agacagacaa tatccccggc
     7501 attcggggca tcggtgaaaa aggcgcgctt cagctcctca cggagtaccc aacgttggac
     7561 gccatctacg agcatctcga tagcgttccg gaaaagctac gtgggaagct aacggagggc
     7621 cgagcggacg cctttctgag ccagcagctg gtgaggttga aagccgatat ccccatttct
     7681 caggattttg aagatctacg ctatcattcg gtgaacccca cccagttgct gcaggatttc
     7741 cgccgttttg agttcagaag tctcgagcga gaatggatgc cgacgctctt cggagcggga
     7801 ggggacggga cgagcgaacg ggaacaggag gcgataccga cgtcgtcggc gtgtcaagaa
     7861 gcgtctcccg ctccaactga agaagaccgg gactatcgcg tcgtcgtgga cgaaataggg
     7921 ttccgtcaac ttctcgaggc gatgcataag gtcgaggatt gcgcgtttga caccgaaacg
     7981 gattccttga acacaagaga tgcgcgcttg gtgggccttt caatttcgat ggcggagcga
     8041 gcgggattct acttgcccgt cgcgcatgtc gagggtccga acctttccga accgctggtc
     8101 gcggagtttt ttaaagcgct caaagagaaa cgggtgatcg gacataatct caaatacgat
     8161 ttcgaggtgg ttgcgcacaa cggatacccg ctgccggagc gctacttcga cacgatgatc
     8221 gcggcgtatc tcttggaccc ggaccgctca cgcttccatc tcgacggcct ggcgctcgag
     8281 gtctcatcgc aagccccgtt gagctacaag gatctgatga gcgcgcatcc cttcgcgcgg
     8341 gacttttctc aaattccgat cgaacaggcc gctcggtatt cggtggagga cgcggacctt
     8401 tccctgaggt tatgccgccg cttcgaaccg gaactgaaag agaacggtct ggacccgttg
     8461 ttcgaaagga tcgagatgcc cttgctgaga gtcctagttg agatggaaac gaccggggtg
     8521 tatttcgacc gcgaagcgct actcgacctt tcggaaagga tgggagcgca gataaaaggg
     8581 cttgaaaaag aggtggtgag cctcgccgga gagcctttca atttggactc gccgattcag
     8641 ttaggaaagg tcttgttcga aaagctgggc ttgccttcca aaaagagcac tgcaaagagc
     8701 aaacgctact ccaccgacaa atcggtgttg gaagagctgg ccggagaata cgagatcgcg
     8761 gagaagatcc tcgagtaccg caagctgaat aagctcaaga ccacgtatat cgacgcgatc
     8821 ccaaacctga tccacccgaa aacgcaacgg gtacacactt ccttcaatca ggcgggaaca
     8881 gcgaccggcc gcttgagcag tagcgaaccg aacctgcaaa accttcctgt gaagaacgaa
     8941 gagggcaaag agatccgcgc ggcgatcaaa cctcagagac ccggctggtt gttgctgagc
     9001 gcggactatt cccaaatcga gttgcgggtg atggccttcc tgtctcagga cacccatttg
     9061 ctcgaagcat tccgaagaag ccaggatatc catcggtaca cggcctccag gctattcgat
     9121 atcccgtggt ccgaagtcga cgaaaatcaa cggcgtatcg gcaagatgat caacttttcg
     9181 atcatttacg gcgtctcgtc tttcgggctg tcctcgagat tgggaattgg gaggaaagac
     9241 gccgaaaaat tcatagacgc gtattttcgc gcctatccgg gcatcaaaga ctatatgaac
     9301 gcggccatcg ctctcgcgaa ttcggacggt atcgtaagaa cgctctacgg caggcttcgt
     9361 aggataccgt tcatcgccgc gaggaacccg gggctacgta aagaggcgga acgggtggcg
     9421 atcaactccc cggttcaagg gacggcggcg gatgtgatca agatcgcgat gatccggata
     9481 tatgaaaggc tgaaaagaga gggattgcag gcgcgcctga ttttgcaggt acatgacgaa
     9541 ttggttttcg agttacctga atccgaaaag gcctccttac aggctatcgt cgccgaagag
     9601 atggaacagg tcctcgtcgg cgagccgcac tctgcatacg gcgcacacgc gggcggaatg
     9661 aaggggattc cgcttctgaa tagcatctct ttaaaagtgg acatagaagt cagtgagcat
     9721 tattagggag ggaaaagcga tgaaatggat cgagaccgta ccgaacttca gtgaaggccg
     9781 acgcaaagaa gtcgtggatg cgatcgtggc gcaagcgaag gcctacgatc acgtgtggat
     9841 actagacgaa tctctggact acgatcataa ccggagcgtc gtgactatgg taggagaacc
     9901 cgagtccgtc ttgaaggcgc tgtttgatat gaccaaaaaa gcctccgagc taatcgatct
     9961 ccgcgttcac cgaggagaac accccaggat gggggccacc gacgtgatcc ctttgatacc
    10021 cttgaggaat gtgaccgttg aagagtgtgt cacacacgcg gccaccttgg gaaagagaat
    10081 agcggaggaa ctggagatcc ccgtatacct ctatgaaaaa tcggccaccc gggatgagag
    10141 aaaggatctc tccaatatac ggcaaggaca gttcgaaggg ttcgcggcga aaatccagaa
    10201 gccggaatgg tgcccggact ttggcagaag ggagatacac ccttccgcgg gcgcctgtgc
    10261 cgtgggagtg cgggaatttt taatcgcctt caatatcaac cttagaaccc aggacctgga
    10321 gatcgccaac aaaatcgcca aagcgatacg caatatcagc gggggatttc ggtatgtcaa
    10381 ggcgttgggg atggaactga aggaaaaaca gatggttcag gtgtccatca atatgaccaa
    10441 ctacaaaaga agtacgctgt tccgcgtcta tgaaatggtg aagttggagg ccgagcggta
    10501 cgcggtcgcg atagacagtt cggaaatcgt aggccttacg ccgttacagg ctctcatcga
    10561 tgtcgcttcc ttctaccttc ggctggacgg cttaaccccg gaaaacgtgc ttgagatgaa
    10621 gatgctggaa attttgatga aagacgcgga cggacaaaag acctcatagt cgatttgaat
    10681 tagcggggtc actgcgaact gaaataaaaa ttgggtgaga tttttccccg tcgctgtttt
    10741 cttaaaaaaa ccggaaacgc aatgattttt agttcatgta gtcatcgcgg ggtaataatg
    10801 aagggactaa gggcgtatcg cgggtttctg taggctggga acgccgaact ttagttcggc
    10861 atgggttcat tgttcactcg gagcaagatc atttttcaag acatgccaac ttaaaagttg
    10921 gcgttcccag gcgctcccaa gtgcagcatc gcggcgtaga aggggaaaag ggcgtatcgc
    10981 gggtttctgt ggactgggaa cgccgtactt tagtgcggca tgggctcatt gttctctcgg
    11041 agcaagacca tttttcaaga catgccaact taaaagttgg cgttcccaag tgcagcatcg
    11101 cggcgtaagc gtcccttctt tatgcgacgg cgcgatacgg gtttgtttat gccttgaaaa
    11161 acagggttcg cacagagggc acagagaaca cagagaacac agagaaaaga ccgaagaaaa
    11221 ctcgtggggc cgacttccag tcgatctgtc tctctgggaa ggcactggga gcgcctggga
    11281 atgctgtact ttagtgcggc gtgggttcat agttctctcg gagcgaaacc atttttcaag
    11341 acataccaac ttaaaagtag gcattcacaa gtgcagcatc gcggcgtaag cgccccttct
    11401 ttatgcggcg acgcgatatg tgtttgcacg gtcctattgc gcgtagaagg ggctaagggc
    11461 gtatcgcgag acttcgtggg cttccgaagt cagcccgctg gttaccccgc g
//
LOCUS       DAOPXN010000066        11368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5808_length_11368_cov_4.883850, whole genome shotgun sequence.
ACCESSION   DAOPXN010000066 DAOPXN010000000
VERSION     DAOPXN010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_11368_cov_4.883850"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="PKM49_05165"
     CDS             complement(<1..680)
                     /locus_tag="PKM49_05165"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNY82237.1"
                     /translation="MEVWVQKYGGTSVANAERIKDVANRIVKTAKTGKKLVVVVSAPA
                     GMTDSLIGKAKEVSHAPNGRELDMILATGEQLSISLLALAIQELGEKAIALTASQARI
                     QTESVHTKARIKSIPTDKLLKELESGKIVIIAGFQGVTDENEITTLGRGGSDITATAL
                     GCALNAGEVEIYTDVDGVYTADPRVVPQAKKIPRISYEEMLEMAGSGAKVLHSRCVEL
                     AAKYRLPI"
     gene            complement(1331..3187)
                     /locus_tag="PKM49_05170"
     CDS             complement(1331..3187)
                     /locus_tag="PKM49_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82238.1"
                     /translation="MGGPGMGPAMMRGGEKAKDFKGTFRKLLKHLRGYRTTIVIVMIF
                     AACSAIFSILGPKIMGRITTSLFEGVMNKVQGRGSGIDFDAIGKTMLFLIGLYGFSSF
                     LNYLQGWMMTGVSMKISYRFRKDISQKINKLPLKFFDRTTQGEVLSRITNDVDTLSQT
                     LNQSLSQMISSVASVIGVVVMMFSISWQLTLIALLILPLSTALISFVVKMSQKYFRRQ
                     QALLGEVNGHVEEMFGAHIVVKAFSGEENSVQTFGELNRKLYSSAWKSQFLSGMMMPI
                     MVFVGNIGYVIISILGGWFAARKIITVGDIQAFIQYIRTFTQPIAQIANIANIFQQTA
                     AAAERVFAFLEEEEETPETPSPTVLTHVVGEVSFNHVRFGYDPEKTVIHDFSSQITPG
                     QHIAIVGPTGAGKTTIVKLLMRFYELDAGEILLDGHDIRNFSRNHLRDQFGMVLQDTW
                     LYNASILENIRYGRLSATDEEVLEAAKAAHVDSFVRALPEGYQMVLNEEATNLSQGQK
                     QLITIARAILSDPKILIFDEATSSVDTRTEVQVQQAMENLMKNRTSFIIAHRLSTIRD
                     ADLILVMNHGDIVEQGTHQALMNQNGFYADLYRSQFEKPLVNNHVGMVTSAD"
     gene            complement(3232..5460)
                     /locus_tag="PKM49_05175"
     CDS             complement(3232..5460)
                     /locus_tag="PKM49_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82239.1"
                     /translation="MLKIIRYVKPYTHFLLLSLFFLFVQAMSELALPDYMSRIVNTGI
                     QQGGIEHSVPTALRKTTLDHLALFMTDDEKSLVFDRYTVVKLGDPRFESAQNTYPILK
                     SEPIALLNDPSDVRSESLSKALNRSLLIISGIEKAKSEAVDGMMDLNGTKISVAVDPY
                     RLIALMGVEKRQEMVKGMDKMFTALGESMVHQGAVAAIKAEYTAMGVATESLQNRYIV
                     RTGLLMLAIALLAALSTITVVFLSAKIGAGVARDLRQDVFSKIEGFSLYEFDRFSTAS
                     LITRTTNDITQIQMVTVMIVRMVFFAPIMGIGGIIRALDKSVSMSWIIALAVIILLGL
                     ISVLFAIAVPKFKKIQLLIDRLNLVSRENLSGLMVIRAFVTQDFEEERFEKVNRELTK
                     TTLFIGRMFAVMMPLMMVIMNGIMLLIIWVGAHQIAESSLQVGDMMAFMQYAMHIIFS
                     FLMLSMMFIMLPRAAVSAGRISEVLETEPTIKDPREPIPFPQGKEEGVVEFQNVSFRF
                     PGAQEDMLKNISFTALPGKTTAIIGSTGSGKSTLLNLIPRFFDVTEGQVLVDGVDVRQ
                     VTQKALRSKIGYVPQKGLLFSGTIEFNLKYANAEAAPEDMETAAQIAQASEFIEAKPD
                     KYQEHIAQAGANVSGGQKQRLSIARALMKKAPIYLFDDSFSALDYKTDMALRRQLQEN
                     TASSTVIIVGQRIATIMNADQILVLDEGRIVGHGTHRELIEHCKTYQEIAYSQLTKEE
                     LA"
     gene            complement(6188..7399)
                     /locus_tag="PKM49_05180"
     CDS             complement(6188..7399)
                     /locus_tag="PKM49_05180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82240.1"
                     /translation="MSFLGQEIKKRLSALGKRKVVVGIPSYNNEGTIAHVVQQAGKGI
                     IEHFDRDGMILNCDGGSTDRTREVFMETDTLSIPKLSFQYEGVPGKGSALIAMVDAAY
                     AVGAQALVFVDSDLRSVTPEWIYRLAKPITDGKASYVTPSYRRHKYDGTITNQICYPL
                     TSVLYGTEVRQPIGGDFGVSRQMMEVYLAEPKETWRGDAARFGIDIWMTTLAINEGQA
                     PVFQAALGAKIHDVKDPGKHLGPMFTQVVGTLFSLMDRYQEKWSAYAQRPVQAPIFGE
                     PVETRVEPLDVDLENLKRKARSGAAKIEAFLRSSLPPEFLAIVDRVLRKGVCDSSDWL
                     RFVFLISKKTQVPSEKEQWMESLIPFYYARVASFVEATLESTEEEAELAIRKQVCLAY
                     TMKGELAEVWK"
     gene            complement(7404..7994)
                     /locus_tag="PKM49_05185"
     CDS             complement(7404..7994)
                     /locus_tag="PKM49_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82241.1"
                     /translation="MECTTVKPLPSGSQQNVRRLGFRVFNPGNTSVLLSSTRALHSCG
                     IRGDRLVPFAGTIRAKAIETIGITFLKRDYPIGKGGSLSLLIPDADWVKPAGEAPVDP
                     TKIQELVRLRDDHEKLASRYRGDEMEASEQDFQSRFAYYRAKRTALELSLSLCQHSRA
                     TTAGDPEIKQLFSELNDLRIKRRSIELLIETLKSEV"
     gene            complement(8005..9897)
                     /locus_tag="PKM49_05190"
     CDS             complement(8005..9897)
                     /locus_tag="PKM49_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82242.1"
                     /translation="MIPQAIFAHTHWDREWFERADATKPLLILLFENLFRMVEEDPKY
                     YFILDGQTVMIQDYLSLLSWSEKKEAVRKLKELTKNLSWGPFYGQIDWRIGEESAIEA
                     LEEGVRQAKVFGSPFQVGWLLDNFGLTSQIPQILNSFGITCAVVWRGVPLESPTTEFR
                     WVSPDGSSARVLYALDSYRNLMRIGDYPEVAQKRVSLAVQRLRPYARSQTLPLFNGYD
                     LDPKPENPSNFGDFVSSDPQTCLAEEGLSVDLPTIRGELRCGRFVCVFPGSLSTRIQL
                     KQMHRKLEDLISRQLQPLNSLRWLLSGVKRSFRLEWQLLLKNLIHDSIGGVGTDVVHR
                     EMESRYQELYERCLQSRQQLLDGLSPYLSEGYYMWNLQPIPITVTGVYQNQYLVCSIS
                     GGRMEKVEPTFEQLSTQEREIKRFYWYNEHYSLLIEEGTVTLIPANGEKRNLQFSIFE
                     DAGDTYSSAFGRKVLYQYSGMTTLQQSVSYRSLKLRFENPLIRWEWIIECDQTPVIKM
                     KANSWGRGVRYALILTLTGKGDLTVFSPFDAFERPSRDFHKEPEEVWQPFLVAARETG
                     RVTEFSFKDFVGYRRDGQTTGWDKRRFPEVEERVFNSADSVSFAESTIQRSERSLHTA
                     AIAHFF"
     gene            complement(9894..10982)
                     /locus_tag="PKM49_05195"
     CDS             complement(9894..10982)
                     /locus_tag="PKM49_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82243.1"
                     /translation="MSEVTVKEVKKYFNQVKALDGVDMKIQDGEFVVLLGPSGCGKTT
                     LLRCISGLETVTSGEIRFDQKNVTHLPPKDRNLAMVFQSYAVWPHMKVKENIKYPMRI
                     KKFSKEEIDKRAKETAELLNITELMDRYPAQLSGGQRQRVAVARAIVLEPAALLMDEP
                     LSNLDALLRVTMRSELKKLQQRIQTTTVYVTHDQVEAMTMGDRIAVMRDGKILQFGKA
                     EEIYQRPKNVFIAGFIGSPQMNFLEMTVKREEGRAELVSKGLSILCEEDKVKGLEKAN
                     LDKVILGIRPENILLSPENGCVPIKGSVYFTERLGGETIVHVSTESKRFVVKVSGDAL
                     FNENETLRIYLNVKKTRLFDPETEEAIG"
     gene            complement(11008..>11368)
                     /locus_tag="PKM49_05200"
     CDS             complement(11008..>11368)
                     /locus_tag="PKM49_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82244.1"
                     /translation="FIIIISSSILGGISVEYEEAGMVFGLTRFRSFLRITLPLALPGF
                     AAAAIYAFVLSWNEVFISAIINLTNRTLPAHILNTAMTSPDYFKFAAGTVMALPAMIF
                     IFFTRKYLMSMWGISLK"
BASE COUNT         2576 a         2984 c         2875 g         2933 t
ORIGIN      
        1 tggatgggga gtcgatattt cgcggcaagc tcgacgcaac ggctgtgcaa aaccttcgcg
       61 ccggatcctg ccatttcgag catttcttca taagagatcc tggggatctt tttcgcctgt
      121 gggaccactc tcgggtcagc cgtatagacc ccgtcgacgt cggtgtagat ctctacctcc
      181 cccgcgttga gggcgcatcc caaagcggtg gccgtgatat cggaccctcc ccttcccagc
      241 gtcgtgattt cgttttcgtc agtcacgcct tgaaaaccgg cgatgatgac gatcttcccg
      301 ctttctaatt ccttgaggag tttgtcggtc gggatactct tgattcgcgc tttcgtatgc
      361 acagattcgg tctgaatccg agcttgagaa gcggtcaagg cgatcgcttt ctcacccaac
      421 tcctgtatcg ctaaggctaa gagagagatc gaaagctgct ctcccgtagc taagatcata
      481 tcgagttcac gaccattggg cgcgtgtgaa acttctttcg ccttacctat cagcgaatcc
      541 gtcattcccg ctggcgccga gacgaccacg accagttttt tcccggtttt ggccgttttc
      601 actatccggt ttgcgacgtc cttgatcctt tcggcgttgg cgacagacgt gcccccgtac
      661 ttttgaaccc aaacctccat aacgatcctc caaaaaaaag ggcgtttctt tacgaaacgc
      721 cctcgaaatt tcagtatctg taagcgctaa acgcgcccac ccaatttcgg gggcgtgtgg
      781 cgcatcatca tatagccttt tcgttggaac gcgtttgcct ggtttttcat tgacgattac
      841 ctcctgattc tgacctatta tatcacgaaa tatcgcgctt gtgaatcccc cgccgtgcga
      901 gaaatttggt cgcttttgcg gggacgtcaa atttttggga acgccctgag aacacgctgg
      961 aaacgtgctg ggcacgcctt gcgtacgccg gactttagtc cggcatgtct gtgatatttc
     1021 cactagagcc gcttttcgta ggagtatccg atctactttt cgcctatctc gtttttcgta
     1081 ttccctccgt gctgccgtga gaacattgtt tttcaagtca tgctaactaa aaagttagcg
     1141 ttcccaggca aaacacgtgc cggactgaag tccggcgctc ctagaaaccc tgccgaacta
     1201 aagatccgcg ttcccaagtc gtttccaaaa accaagccga accgaagttc ggagccttca
     1261 aggcgttccc aagacattcc cagaagttcg acgcacacag agggactcga tacggcacag
     1321 taaacatcga ttagtcggcc gaggtcacca tacctacgtg gttatttacg aggggttttt
     1381 cgaattggct tcgatagagg tcggcataaa acccgttttg gttcattagc gcttggtggg
     1441 tcccttgctc gacgatgtcg ccgtggttca ttacgaggat gaggtcggcg tctcgaatgg
     1501 tcgagagtct gtgggcgatg atgaagctcg tgcggttttt catcaggttt tccatcgcct
     1561 gttgaacttg gacctccgtc cgtgtgtcga ccgagctggt ggcttcgtcg aagatcaata
     1621 tcttcgggtc cgaaagaatc gcgcgggcga tcgtgatcaa ctgtttttgc ccttgcgaga
     1681 ggttcgtggc ttcttcgttc agtaccatct ggtacccctc gggcaacgcc cggacgaatg
     1741 aatccacatg cgctgctttc gcggcctcga gaacctcttc atctgtggcg ctcaatcgcc
     1801 cgtaacggat gttttccaag atcgacgcgt tgtagagcca cgtgtcttgc aggaccattc
     1861 cgaactggtc gcgtaagtgg ttacgtgaga agttccgtat gtcgtggcca tcgagaagga
     1921 tctcacccgc gtctaactcg taaaatcgca tcagcaactt gacaatcgta gttttccccg
     1981 cgccggtggg cccgacgatg gcaatgtgct gcccgggagt aatttgtgag gaaaagtcgt
     2041 ggatcacggt cttctcaggg tcgtagccaa accggacgtg attgaaagag acttcaccaa
     2101 cgacgtgagt gagaaccgtc gggctcggtg tttcgggggt ttcctcctcc tcttccagga
     2161 aggcgaagac tctttcggcc gcggccgccg tttgttggaa gatgttggcg atattggcga
     2221 tctgcgcgat cggttgggtg aaggttcgga tatattggat gaaagcttgg atatcgccga
     2281 cagtaatgat ttttctagcg gcgaaccagc ctcccaaaat gctaatgatg acgtatccaa
     2341 tgttgccaac gaagaccata atgggcatca tcatcccgga taggaactgg gatttccagg
     2401 cggaagagta gagttttcga ttcagttctc cgaaagtttg aacgctgttc tcttctccgc
     2461 tgaaggcttt cacgacgata tgggcgccga acatctcttc cacgtgcccg ttcacctcac
     2521 ccaaaagggc ttgttgtcgc cggaagtatt tttgggacat tttgacgacg aagctaatca
     2581 atgccgtcga aagcggaagg atcaacaggg cgatgagcgt cagttgccag ctgatactga
     2641 acatcatcac cacaactccg atgacgctgg cgacggagga gatcatctgt gaaaggcttt
     2701 gattaagcgt ttgactgagc gtatcgacgt cgttggtgat ccgggagagc acttctccct
     2761 gtgtggtgcg gtcgaagaat tttaacggga gcttgttgat cttttgggag atgtctttcc
     2821 ggaatcgata agagattttc atcgatacgc ccgtcatcat ccaaccctgg aggtagttta
     2881 agaacgagct gaagccgtac aatccgatca agaatagcat cgtttttccg atcgcgtcga
     2941 agtcgatacc actccccctt ccttggactt tgttcataac gccttcgaag agggaagtgg
     3001 tgatacggcc cataatcttc ggccccagga tagagaaaat cgcgctacaa gcggcgaaga
     3061 tcataacgat cactatggtc gttcgatatc ctctcagatg tttgaggagt ttccggaagg
     3121 tccccttgaa gtctttggcc ttctctccgc ctctcatcat cgccggcccc atacccggtc
     3181 ctcccatccg cgcgcgggga gtcggtttct ttgtggattc tcgttcagag ctcacgccaa
     3241 ttcctccttc gtgagttgag agtaggcgat ctcttgatag gttttgcaat gttcgatcag
     3301 ctctcggtgt gtaccgtgcc ccacgatccg cccctcatcc aataccagaa tttggtcggc
     3361 gttcataata gtcgcgatgc gttgccccac gataatgacg gtgctggacg ccgtgttctc
     3421 ttgcaactgc cgtcggagag ccatatctgt tttgtaatcg agagcggaga agctgtcgtc
     3481 gaaaaggtaa ataggcgcct ttttcatcag cgcgcgggcg atcgagagcc tctgtttctg
     3541 tccgcctgac acgttcgcgc cggcttgggc gatgtgttcc tgatacttgt ccggtttcgc
     3601 ttcgataaac tccgacgctt gcgcgatttg cgcggccgtc tccatatctt caggagcggc
     3661 ctcggcgttg gcatatttca aattgaattc tatcgtaccg ctgaacagca atcccttctg
     3721 tggtacgtac ccgattttgg agcgaagcgc tttttgggtg acttgccgca catccacgcc
     3781 gtccaccaac acctgtccct ccgtgacgtc aaagaacctc gggatcagat tgaggagcgt
     3841 cgacttgccg gaaccggtcg agccaatgat ggcggtcgtt tttccgggta atgcggtaaa
     3901 agagatgttt tttagcatat cctcttgggc cccagggaat ctgaaggata cgttctgaaa
     3961 ttcgaccaca ccctcttcct taccttgtgg aaacggaatc ggctcgcgcg gatctttgat
     4021 cgtgggctcg gtctccagga cttccgaaat acgaccggcg gacaccgccg cgcgcggcag
     4081 cataataaac atcattgaca gcatcaagaa agaaaaaatg atgtgcatcg cgtattgcat
     4141 aaaggccatc atatcgccaa cctgcaaact ggattcggcg atttggtgcg cgcctaccca
     4201 gatgatcagc aacattatcc cgttcataat gaccatcatc aacggcatca ttacggcaaa
     4261 catccgtccg atgaacaacg tcgttttcgt gagttcacgg ttgacctttt caaacctctc
     4321 ctcttcgaag tcttgtgtga cgaaagcgcg aatgaccatc agcccggaca ggttttctct
     4381 agagactaag tttaagcggt caatgagtag ttggattttt ttaaatttcg gcacggcaat
     4441 agcgaacaag accgagatca gccctaacag aatgataacc gcgagcgcga tgatccagga
     4501 catcgaaacg ctcttgtcca aagctcgtat gattcctccg attcccatga taggcgcgaa
     4561 gaagaccatc cgaacgatca tcactgtgac catctggatc tgtgtgatat cgtttgtggt
     4621 tcgtgtgatt aatgaggcgg tggagaacct gtcgaactcg taaagagaaa aaccttcgat
     4681 cttcgaaaaa acatcctggc ggaggtctct ggctacgccc gcgccgattt tcgcggaaag
     4741 aaagactacc gtgatggtac tcagcgcggc aagcagcgcg attgccagca taagaaggcc
     4801 cgtgcggacg atgtaacggt tctgcagaga ttcggtcgcc acgcccatcg cggtatactc
     4861 agctttgatg gccgccacgg ccccttgatg aaccatactc tcgccgaggg cggtgaacat
     4921 cttatccatt cccttcacca tttcttgtct cttttcgacg cccattagtg cgatgagccg
     4981 gtaggggtct acggcgacgg agattttcgt gccgttgagg tccatcatac cgtcaaccgc
     5041 ttccgatttc gctttttcga taccggaaat gatgagtagg gatcggttca gcgccttact
     5101 tagagattcc gaccgtacat cggacgggtc gttcagaagg gcgatgggct cgcttttcaa
     5161 aatcgggtaa gtgttttgag ccgattcgaa acgcgggtct cccaatttca cgacggtata
     5221 ccgatcgaat accaagcttt tttcatcgtc ggtcataaac aaagcgaggt gatccagcgt
     5281 ggttttccgc aacgctgtcg ggacggagtg ttctattccg ccttgttgta taccggtgtt
     5341 cacgatacgc gacatatagt ctggtagggc cagctcgctc atcgcttgga cgaaaagaaa
     5401 aaagagagag agtaaaagga agtgtgtgta aggctttacg tatcgaatga tctttagcat
     5461 aacgaaaact ccttaaattg ggattcctgc gctttcgaaa agccgctact attcgactgt
     5521 ttggaagaac gaaaagttgt aggctcggtt tttccgcaga tggccggtac aatggggaag
     5581 gtcgaccgtc cttttcattg aacaaaacag gcggttgagg aaaagggcgg atcccgggga
     5641 aatcgagacc acgccgtcta tcttaagcta ttcatatgga gaatgtcaag aggaagatgc
     5701 ggctttcgaa aaagtcgtta gggcgtaagc gcgaaagggc aagcgcagat cgcggggtcg
     5761 taaaaaccac ttaccccgcg ataacctcac acgggcatcg cggcgtaagc gcttcttctt
     5821 ttcgcgacgg cgcgataccc ttttgagaaa gacgtagggc ggatcgcggg gtcgtaaaaa
     5881 ccacttaccc cgcgataacc tcacacgggc atcgcggcgt aagcgcttct tcttttcgcg
     5941 acggcgcgat acccttttga gaaagacatt gggcggatcg cggggtcgta taaaccgctt
     6001 actccgcgat gaacctcgta ttcgctttcc gcgccctccg cgtgaacccc gcttttgctc
     6061 tttgaaaggc atgccaactt aaaagttggc gttcccaggc gttcacaaaa tcccggccat
     6121 ctcagggagc tcccaaaagt ccggcgctct gaggacgttc ccagcggttt ttccctcagt
     6181 gaacggttta cttccagact tcggcgagtt ctcctttcat tgtgtaggcg aggcacactt
     6241 gttttcgaat tgctaattcc gcctcttctt ccgtagattc cagcgtggct tccacgaaag
     6301 aggcgacgcg ggcgtagtag aacgggatga ggctttccat ccattgctct ttctccgaag
     6361 gtacctgcgt tttttttgag atgagaaaga caaagcgaag ccagtctgat gagtcacata
     6421 cgcctttacg taacacccgg tcaactatcg cgagaaactc gggaggcaag gaagatctta
     6481 agaaggcttc tatttttgct gcgccggaac gagcctttcg cttcaggttc tctaaatcca
     6541 catccaaagg ttcaactcgc gtttccactg gttctccaaa tatcggcgct tgaaccggtc
     6601 gctgcgcata cgcgctccac ttctcttgat accggtccat taaggagaag agggtgccca
     6661 cgacctgtgt aaacatcggc ccgagatgtt tgccggggtc tttcacatcg tggatctttg
     6721 cgcctaaggc ggcctgaaaa accggcgctt ggccttcgtt gatcgccaac gtcgtcatcc
     6781 agatgtcaat accaaatcga gctgcgtcgc cacgccacgt ttccttgggt tctgcaaggt
     6841 acacttccat catctgtcta gagacgccaa aatcaccgcc tatcggttgc ctgacctctg
     6901 ttccatagag cacggaggtg agtggatagc atatttgatt ggtgatggtt ccgtcgtatt
     6961 tatggcgtcg ataagaaggc gtcacatatg aggccttccc gtccgtgatc ggcttggcta
     7021 atctgtagat ccattccggc gttacacttc tcagatcgga atccacgaac accagcgcct
     7081 gcgcgcctac cgcataggcg gcatcgacca tagcgatcaa ggcgcttccc ttgcctggta
     7141 ccccttcata ttgaaagctc agtttcggga tagatagggt gtcggtctcc ataaacactt
     7201 cacgtgttcg atccgtacta ccgccgtcgc agttgaggat catcccgtcg cgatcgaagt
     7261 gttcgattat ccctttgccc gcttgctgga cgacgtgagc gatcgtccct tcattgttgt
     7321 agctcgggat accgacgacg accttccgtt tgcccagcgc actcagccgc ttttttattt
     7381 cttgccctaa aaatgacatt gtgttacacc tccgatttga gtgtttctat caatagctct
     7441 atgcttctgc gtttaatgcg taaatcattc agctccgaaa acaactgctt tatctctgga
     7501 tcgcccgccg tagtcgcccg agaatgttgg cagagtgata acgagagttc taacgctgtc
     7561 ctcttagccc tataataggc gaaccgactt tggaaatcct gttcggacgc ttccatttcg
     7621 tctcctcgat aacgacttgc gagcttttca tgatcgtctc gaagacgtac caactcttgt
     7681 atcttcgttg gatcgaccgg agcttcccca gcgggtttca cccagtcagc gtcaggaatg
     7741 aggagcgata gagacccgcc tttgccaatg ggatagtctc gtttgagaaa cgttatccct
     7801 atggtttcga tcgccttcgc tcggatagtc ccggcgaagg gtactagtcg gtctcctctt
     7861 atcccgcaag aatgaagggc gcgcgttgag ctaagcagaa cggaggtgtt cccggggttg
     7921 aagacccgaa accccagcct cctgacgttc tgctggcttc ccgatggcag gggcttcacc
     7981 gtcgtgcatt ccacatatcc gccttcaaaa aaagtgagcg atagcggcgg tatgaaggga
     8041 gcgctcggac ctttggattg ttgattctgc gaacgaaaca gaatcggcgg agttgaaaac
     8101 gcgctcttcc acctcgggaa atcttcgctt gtcccaccct gttgtttgcc cgtctcgccg
     8161 gtatcccacg aagtctttaa aggaaaactc ggtgacccga cccgtttctc gagccgcaac
     8221 caagaagggt tgccagacct cctccggttc tttatggaaa tcccgggatg gccgttcaaa
     8281 ggcatcgaag ggagaaaaaa cggtcaagtc gccttttcct gtcaacgtca gaatgagcgc
     8341 gtatctcact cctcgccccc acgaatttgc cttcattttg atcaccggcg tttggtcaca
     8401 ttcgatgatc cattcccaac ggatcagtgg attttcaaag cggagcttaa gggacctata
     8461 ggaaacgctt tgttgtaatg tggtcatccc cgagtattga taaagtacct ttcgtccgaa
     8521 agctgaagag taggtgtccc ccgcatcttc aaaaatggaa aactggaggt tccgtttttc
     8581 cccgtttgcg gggatgaggg tgacggttcc ttcttctatc agcagactat agtgttcatt
     8641 ataccagtag aatcgtttaa tttctcgctc ctgcgtggat aactgttcga acgtcggttc
     8701 gaccttttcc atccttccgc ccgaaatcga acacacgaga tactgatttt gataaacccc
     8761 tgtcacggta atcggaatcg gttggaggtt ccacatataa taaccttccg atagataggg
     8821 agataggccg tccagaagct gttgacgtga ttgcaggcat cgttcgtata gctcctggta
     8881 acgagattcc atctctcggt ggacgacatc ggttccgaca cctccgatgc tgtcgtggat
     8941 gaggtttttg aggagaagtt gccattccaa cctaaaggat ctcttaaccc ctgataacag
     9001 ccaacgcagg ctgttcagcg gctggagctg tcgagaaatc aagtcttcca atttccgatg
     9061 catttgtttg agttggatcc gggtagataa agagccgggg aagacgcaga cgaagcgtcc
     9121 gcaccttaac tccccgcgaa ttgtgggaag atcgacgctt aacccttctt ccgcgagaca
     9181 agtttgcgga tcagatgaga caaaatctcc gaaatttgat ggattttccg gtttcggatc
     9241 aagatcgtac ccgttgaaca atggaagcgt ctgagatcgg gcataaggtc tgagcctctg
     9301 aactgccaaa cttacccttt tttgagctac ctccgggtaa tcgccgatgc gcatcaagtt
     9361 tcgataagag tccaaggcgt agaggactcg cgcgctcgat ccgtcgggag aaacccagcg
     9421 gaattcggtt gtcggactct ctaacggaac tccccgccat acgacggcgc aagtaattcc
     9481 aaaactattc aggatttgtg ggatttgaga agtcagccca aagttgtcca acagccaacc
     9541 aacttgaaaa ggactcccaa aaaccttcgc ttgacggacc ccctcttcca gcgcttcgat
     9601 cgcggattct tcaccaatcc gccagtcgat ttgaccataa aacggccccc aggagaggtt
     9661 cttcgttaac tccttgagct ttcggacagc ctcttttttt tcggaccagc tgagcagcga
     9721 tagataatcc tgtatcataa cggtttgccc gtcgaggata aaatagtatt tcgggtcttc
     9781 ttctaccatc cggaacaaat tctcaaacaa cagaatcaac agaggttttg tcgcatctgc
     9841 ccgctcaaac cattcccgat cccaatgggt atgcgcgaag atggcttgtg ggatcaaccg
     9901 atagcctctt ctgtttcggg atcgaataga cgcgtctttt tcacattcaa gtagattctc
     9961 agggtctcgt tttcattgaa tagtgcgtcc ccagagactt tgacgacgaa acgtttgctt
    10021 tcggttgaaa cgtgaacgat cgtttctccg cctaaccgct ccgtaaaata aaccgaaccc
    10081 ttaatgggta cgcaaccgtt ttccggtgac aacaggatgt tttcagggcg tatgcccaag
    10141 atcactttgt cgagattcgc cttttcaaga cccttgacct tatcttcctc acagaggatc
    10201 gaaaggcctt tactgaccag ctcagcgcgt ccctcttccc ttttcaccgt catttccagg
    10261 aagttcattt gaggagagcc gatgaatcct gcaatgaata cgttcttggg acgctggtag
    10321 atctcctccg cttttccgaa ctgtagaatc ttcccatcgc gcatcaccgc tatacggtct
    10381 cccatcgtca tcgcttccac ttggtcgtgg gtgacgtaga cggtggtcgt ttgaattctt
    10441 tgctggagct tcttcaattc gctgcgcatc gtaacccgga ggagggcgtc gaggttggac
    10501 aagggctcgt ccattaacaa agccgcgggc tcgaggacga tcgctctggc caccgctacc
    10561 cgttggcgtt gccccccaga gagttgtgcc ggatagcgat ccatcagctc ggtaatattc
    10621 aggagctctg cagtctcttt ggctcttttg tcgatctctt cttttgagaa tttcttgatt
    10681 cgcattgggt atttgatatt ttctttcact ttcatatggg gccagacggc gtagctttga
    10741 aaaaccatcg cgaggttccg gtctttggga ggtagatggg tgacgttctt ttggtcaaaa
    10801 cgtatctcgc ccgaagtaac ggtttccaac ccggagatac aacgcagaag ggttgttttt
    10861 ccgcatcctg atgggcccaa aaggaccacg aattcgccat cttggatttt catatctacg
    10921 ccatccaagg ctttgacttg attgaagtat ttcttcactt ctttcacggt gacttcggac
    10981 aaccaaaatc cctcctctgg tttcgcctta tttcagtgaa attccccaca tactcatcag
    11041 gtatttccgg gtgaaaaata taaaaatcat cgccggaagc gccataacag ttccggcggc
    11101 gaatttgaag taatcgggtg aggtcatcgc cgtgttgaga atgtgggcag gtaaggttct
    11161 gttggttaaa ttgatgatgg ccgaaatgaa cacttcattc caggaaagta caaaagcgta
    11221 gattgcagcc gccgcgaaac caggcagcgc cagaggcaaa gtaatccgaa ggaacgatcg
    11281 aaaacgggtt aaaccaaaga ccattccggc ttcttcgtat tccacagaaa tgccgcccag
    11341 aatgctggag gaaatgatga tgataaat
//
LOCUS       DAOPXN010000067        11296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_5837_length_11296_cov_4.924740, whole genome shotgun sequence.
ACCESSION   DAOPXN010000067 DAOPXN010000000
VERSION     DAOPXN010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5837_length_11296_cov_4.924740"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..135)
                     /locus_tag="PKM49_05205"
                     /pseudo
     CDS             complement(1..135)
                     /locus_tag="PKM49_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaI"
     gene            complement(2820..3467)
                     /locus_tag="PKM49_05210"
     CDS             complement(2820..3467)
                     /locus_tag="PKM49_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82245.1"
                     /translation="MPPKKKKENKKLKIVALIVILCAVMVVMVFYTLPTLGINLFPTN
                     SKPKAAVAPVRPVGAVTTSPSAGAPVPVQAGVDAALIRQSVSELSQVDKSTRYDSEII
                     LPYLLSLDNPEEIRRLLTSKTPGLATFPEIVYLSCFTDRSGDKTGWIRSRERENEIIE
                     IKVNHPLPGYPDTQIIDINEVGILIYRYPDVNKKPEDQNPQLYRILSRAYKDFQL"
     gene            complement(3475..3963)
                     /locus_tag="PKM49_05215"
     CDS             complement(3475..3963)
                     /locus_tag="PKM49_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82246.1"
                     /translation="MAFNRIFGILIAIGIFIASIIFFAILPTLEFRKQKEALTLIAQV
                     YEQTWRQHENESNQYTQYARLIDSQSNEFDQLVAVLRNYNLKPTLSGKSLTVSGKVSA
                     QAFSELLSAFGNAYTLEINRFNASNSEKMPIQISESKMTTFDIRNLEVKSIQYNEKLS
                     KR"
     gene            complement(3976..4569)
                     /locus_tag="PKM49_05220"
     CDS             complement(3976..4569)
                     /locus_tag="PKM49_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82247.1"
                     /translation="MININLYKNKKPKLKITTILFLVAILLLPVLGYRITLTTLKNNE
                     MQKIRLANPQSGVVLRETPISDYDDSIRLLTLKDEETKTQISILQNERRNLERALVYA
                     MITRKFFKAVDASYHKHSQEERVFVESIQIERKSKIEIDFFDIQLKAGNTQFLFFQDV
                     LSSIDRIIGNTRNQSQQIGFVELSALKAKITVDTSKF"
     gene            complement(4526..5671)
                     /locus_tag="PKM49_05225"
     CDS             complement(4526..5671)
                     /locus_tag="PKM49_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82248.1"
                     /translation="MQIFNRRVLALETNPFITKALLLHLSGKRAKVLKKIIKTHPSFT
                     DEILSDIQVTHFKKVVEALSPDAEDFVVLNFPSEKLITAVEQMPALSEEQLANAIKFK
                     KAEEYSISVSDLVVKVAQPLRDTRSLFEKTRHLAFATKRVSIDQYLSRLFSEAKAPEP
                     DVLLPDSIKYFELIDNKAFLGSMKTGDRFRFLALLDLQYSLLFAFLNGSIYHVAEIPI
                     TIKTLLERLRTLDVDVEEVLEAIVKGAEIGSLGYVKDIEPVIDETYRNFLFETEKAIR
                     LVLNNSRIANAINQVDALSLLSLNHRITMDLEMIARNMRLLNGAPITRIPLKPDFPED
                     LDLYRTVVGLGYRGIREIGKYKFVKEIERGGRGFDKYKPLQKQKTKA"
     gene            complement(5690..6076)
                     /locus_tag="PKM49_05230"
     CDS             complement(5690..6076)
                     /locus_tag="PKM49_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82249.1"
                     /translation="MERRKGWSLVEFILYLLITVSVETAFLTHWTSSLSHSAVGTPES
                     FVAAIERQMARVRCEALIEIKQKALFIRKDTVICGFRVMQDFQILFNHFGVIEKGGSV
                     GVAKGNDFFRLTISPVTAGIRFFKVP"
     gene            complement(6104..7297)
                     /locus_tag="PKM49_05235"
     CDS             complement(6104..7297)
                     /locus_tag="PKM49_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNY82250.1"
                     /translation="MPNYSYRATDSSGNRITGVVNSANEATAVTSLINRGYIVLSLET
                     IAEKKIKRIMTLRLLDIMTFSRQLATMIEAGVNLANALATLAEQEVFSPKFRKIVTSV
                     LSNIEAGMSFSEALDNEKAFDDLFVNLIEAGETSGTLAETLEKVASFYESQKRLKDEI
                     KSATSYPVFVMGFSVIMVIAILAFVLPKLVEAFGGEAEGIMAILMQASNFLRGNWLIL
                     LLIVVGLGIFLAYYLKTFAGKKALSYILSVIPAVKNIRRNSSLERYCRTLSVMVGAGV
                     EILRALELASKAANDYRFEKSAQEMAERIKTGETLEQAFTEAELFPGIVIAMVGTGEK
                     TGKLDKVLDKVGDFFEERVRTSVKQLVSMLEPAMMVVVGGFIGLIAYAMYSSMFSGQQ
                     NLSRF"
     gene            complement(7320..8417)
                     /locus_tag="PKM49_05240"
     CDS             complement(7320..8417)
                     /locus_tag="PKM49_05240"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNY82251.1"
                     /translation="MRRGKWLYILIFFVLGTSLLASAYKGLTFEEWLQVLNHGNTAEK
                     RVALEAMGYLYEPQVEDLLLTYLKNENRELRLSAVWSLGQIRSLLALEPIVWMLGSDD
                     DVRIDSALINFGELAIPHLIVKLKLIGIMQDYEQGLRVSRIIIDINSPDGIVEVANFL
                     TNLLTNEGYKSKATSMLSQIGEKAIAPLMVAFQKTYSDDVKINILNTLVTIGSEKALE
                     VLYSQAESFNPAVRRTVANALGRIKAPGNLPYLIAFLRDTDENVRMQAVIALGELKDP
                     GASLYLINLLSDPNAVIKAFAAYALGEIKDVRAVYPLFALLNDTTLLKSVDPKTGKSI
                     EMAVYKFAVEALKKINDPRGLDELKRRGLDK"
     gene            complement(9292..10233)
                     /locus_tag="PKM49_05245"
     CDS             complement(9292..10233)
                     /locus_tag="PKM49_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNY82252.1"
                     /translation="MYEGLEMRKVYAELLIEHAQKDDRIVLLEADLMSSTGTKAFKER
                     FPDRLINVGVAEANLMGISAGLAVMGMIPFAHSFTAFATRRAFDQVTISIAYSRLPVK
                     IGGADPGVSAELNGGTHMSFEDAGIMRNLPGMTIVEPVDSVQLAALLPQVISHPGPVY
                     MRLHRKNKEVLFSPDRSFTIGKIETLETGEDVTLICSGMMVKQSLKASEILRKEGVRP
                     RVLNMHTLKPVDATTIIQAARETGAIVVAENHSVLNAWGSAVAEVVSEHCPVPVFRIG
                     VQDHFGEVGKSDFLMEKYHMTAADIVQAAYSAMDKKR"
     gene            complement(10236..11090)
                     /locus_tag="PKM49_05250"
     CDS             complement(10236..11090)
                     /locus_tag="PKM49_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNY82253.1"
                     /translation="MDATQTNALVSKANEIRRLILEMIGKLGVGHIGGSLSIVEALTV
                     LYYKHMTVDPQNPLWEKRDRFVLSKGHSGPGLYAVLADKGYFPRSWLDTLNRPNTNLP
                     SHCDRLKTPGIDMTAGSLAQGFSAAVGMALAARMQGQGVRVYALIGDGESQEGQIWEA
                     AMFAGNHGLDNLIAFLDYNNMQIDGLVSEINSLEPVADKWRAFGWNVYSVDGHDIVAI
                     DSALTLGKRFKGRPTMIILNTTKGKGAFFSEGKVSSHNMVVTDEQWREAVERLRQEGE
                     PVTSGEGR"
BASE COUNT         2433 a         3090 c         2908 g         2865 t
ORIGIN      
        1 cttgatgccc cagcgcatgc cttgcgcgct gttcgggctg tcggcatcgg gtggcccgaa
       61 catggcgaga cacttccacc agaaacgatt gaccgcatcc tgaaccattg ccttttgttc
      121 ttcggtaccg gtcacgaagt accctcccgg atcgaatcgc tcgatctcta cgcccggaac
      181 cttcacggcg atgagtttgt ccaacgcctc gtcatcgagc gaggtgatgt ttatccgcac
      241 cagctctgtc cgcacaaccg gttgacccac gatgcggcca acgtcaatct cgctcaattc
      301 gtcgacgaag gcgcggacgt acttttccgg accataaaac ttgtaggcgc ccagggattc
      361 gatgtagatg atcttgacgt tatgcttcat ataaaattgt tcccagatga gcgagatgtt
      421 cggggctttc aggtattcga aataggcgag ctccgagtag atcgtctcgt tcgcggcctc
      481 ccaatccaag cggcgtagct cttcagcagc ggccttaatg tcggcggcgt tttcacctcg
      541 gagtatgaat aggtcgagga agtacaccgc ggatacgttc acgctcggcc agcgtttggc
      601 gagataggtc gcgatgtctc cctgcccgga tagtaagttc gaagcgacga cgttgacctg
      661 cccgagctcg atgtatgagg tttttccagc ggaggttgaa ccggtcgctg acggtgtggc
      721 ggggctctgc gtttccgtgg ctgtggctga agatccttgc gcgctggcag aacccgcgct
      781 ttctgtgatt tttacctggg ttgtcataaa tttatccgct tctttcgcca tcagctcttg
      841 gctcacgtta accacttcga cgtattcagg ctttttgaac cagacctgct cgagggtcgt
      901 caagtatccg gcgatcgtgt cgaggtcctg tggcttcccc cgaaggatga tcagaggctc
      961 gaaagacggg tcgatctgta ccgacgggta cttcgccacg aggctgttcg gactgtcggg
     1021 gttcagtaag gcggttcggg cgttgtccgg gaggttttcg gacaggacga tcatccgtat
     1081 ctccgactcg agcgggggca cgagtatgcc ccagtcgtcg gctatgcgct tggccgttat
     1141 cacttcgtag ttcgagccga ggatcgtgat ctcgtctggc tctccgtcgt agatctgctg
     1201 tagcttccgc aactcctcga ggtctttgtc ggtccattcg aaggtgggtt tacccgcgag
     1261 ctgcgtctgg gcgctcatcc cttcgaagtt tacgagtgcc ttcgggaagg tccgattcaa
     1321 cgccaacgcg aagatcatcc ccgccccgtc tttgaccctg aaacgctcca tcttttcgtt
     1381 tcctcgggct tttcggtctt tgatgtaatc gctgaggtat tgcgccagct tcttgacctg
     1441 tggctcttct ccatagatgt agacggtcgc gttcgcatcc cgtatctcga agcgcatcgt
     1501 gtcaagccgc gggaagcttt cgttcggaga gaaaatgtct ttcaccgtct tggtgtagaa
     1561 ggtcatgaag gcccgcaatt cgtcggtcga aagcggggcg tattcgattt tctgggagta
     1621 ctcggttacg gtttgcgccg tctcggtttc cgcgcctgct tgtcccatcc cctcgaagaa
     1681 gcccataaag tattcgaact tctcttgcag cctcgggttc ccgtacagca aaatgccgat
     1741 cgaaacctct tccggcccgc ttttaccgcg aatcagcacg atgaggtcgt tttgtaggga
     1801 ggagggcagc ttcgcgtact tttctccaaa ttcgtagatg agaaattgag tgaaagcgtc
     1861 tttgttgaag acctgctgtc tcgcggtgtc ttgaaagtag gagaagtttt ggaagagttc
     1921 ggagtccttc ttcaggttga agttgtagga ggcccagtaa ctggtgtcgg ttttcgggga
     1981 gaggttgacc tctttcaaga cctcctgaac tttttccgag atcaggtctt ttctcacgac
     2041 gtagaccgtg ttgttcggca gaaacgcgta gcccaaagag ggattgttca tcataatctg
     2101 gaagaagtag tcctctgctt tcaggctttc ggaaagcatc acgaattcgg cttgcaaccc
     2161 gcgtatgctg gcttcgtcga tgaccaagtt cacgtccagg tacttctgaa gtcgcgccag
     2221 caaggacgtc aacggcggac ggctccccga ctgcgcgctg tcggagtaga tcgtcacctc
     2281 gtcccacgcg acagtcgtcc tgtaaaaggt cacacggagg gtgttcgcga ccacgacgat
     2341 ctccggctcg cgcggcagga gcatgtcgaa gcgcagggtg atcacccctt ttccagacga
     2401 ggcacggaac cccacgatcg gcgaataccc gaggaagcgg ttgatttcca cgtctttcag
     2461 cgacccggga agggtgattt cgtagtagcg gccgtagtcg agcgcttgtg tggcgtattt
     2521 cgtgggttcg cttccgaaga tgaatacgaa gtccaacgcg tcttctgacg gaaagtacgt
     2581 gtagttcacg aggtcggcgc ctaacaccgc ccctgccaag agtaatccca ccatcaaaaa
     2641 gaaacggaga taaaacataa cggttctctt cattttttaa acccccctcg acactcattc
     2701 atatcgcatc tgataaaaaa gtagaaattc ggacagtgtg ccctgtatat gactaatacg
     2761 cgccccgtca acttccggct tctacgggga ttttggtttc aagccgggcg ctttgtctcc
     2821 tacaactgga agtccttgta tgcccgggac agtattcggt acagctgtgg attctgatcc
     2881 tccggcttct tgttcacgtc ggggtagcgg tagatcaaaa taccgacctc gttgatgtcg
     2941 atgatttgcg tgtccggata cccgggcaac gggtggttca ccttgatttc gatgatttcg
     3001 ttctctctct cgcgcgatcg tatccacccc gttttatctc cggaacggtc tgtgaagcag
     3061 ctcagataga cgatctccgg gaatgtggcc agacccgggg ttttcgaggt gaggagcctc
     3121 cggatctctt cggggttgtc caagctcaac aggtacggga ggatgatctc ggagtcgtat
     3181 cgggtgctct tgtctacctg ggagagctcg ctcacgctct gacgaatcag agcggcgtcc
     3241 acacccgctt gaacgggtac gggagcgccg gcgcttggac tcgtcgtcac agctcccacc
     3301 ggccgaacgg gtgcaaccgc ggctttcggt ttcgaattag tcgggaatag gttgatccca
     3361 agcgtcggga gggtgtagaa aaccataacc accatcacgg cgcacaatat cacgattagg
     3421 gcgacgatct tgagtttttt gttctctttc ttctttttcg gaggcatcgg acgatcacct
     3481 cttgctgagt ttttcgttgt actggatcga cttgacctct agattccgta tgtcgaaggt
     3541 cgtcattttc gactcgctga tctgtatcgg catcttttcg gagttgctcg cattgaaccg
     3601 gttgatctcc aaggtgtagg cgtttccgaa ggcgctcaaa agttcggaga aagcctgggc
     3661 gctgaccttt cccgaaaccg tcaacgactt tccgctcaac gtcggtttca ggttgtagtt
     3721 ccgcaaaacg gccaccagtt gatcgaactc gttgctttgg ctgtcgatca atcgggcgta
     3781 ctgcgtgtat tggttgcttt cgttctcgtg ttgccgccag gtctgttcgt atacctgcgc
     3841 gatcagggtc aatgcttcct tctgttttct aaactccaac gtgggcagaa tggcgaaaaa
     3901 gataatggag gcgatgaata tccctatggc aatgagaatt ccgaatatcc tgttgaaagc
     3961 catatctttc accccctaaa acttgctcgt gtcgactgta attttcgctt tcaaggcgga
     4021 gagttctaca aaccctatct gctgagactg attccgtgta tttccaatga tccgatcgat
     4081 actgctcagg acatcttgga aaaacagaaa ctgcgtgttt ccggctttaa gctggatatc
     4141 gaaaaaatcg atctcgattt tggacttcct ttcgatttgg atgctctcga cgaaaacccg
     4201 ttcctcctgg ctgtgcttgt ggtagctcgc gtcaaccgcc ttgaaaaact tccgagtgat
     4261 catcgcgtac acgagcgcgc gttcgaggtt tcggcgttcg ttctgaagga tagaaatttg
     4321 ggtcttcgtc tcctcgtctt tcaaggtcaa caaccggatg ctatcatcgt aatcggatat
     4381 gggtgtttcg cgtaagacta cgccgctttg cgggtttgcc aagcgtatct tctgcatttc
     4441 attgttcttc agggtcgtga gggtgatgcg ataccccaat accggcaaca gtaggatcgc
     4501 taccaaaaac aagatcgtgg tgatcttaag ctttggtttt ttgtttttgt aaaggtttat
     4561 atttatcaaa acctcttccc ccccgctcga tttctttcac aaatttgtat tttccgattt
     4621 ctctaatgcc acgatagccc agtccgacca ccgtccgata aaggtccaaa tcctccggga
     4681 agtctggttt cagaggtatt cgagtgatcg gcgcgccgtt caacaaccgc atattgcgcg
     4741 cgatcatctc gaggtccatt gtgatgcgat ggttcaacga gaggagggac agagcgtcca
     4801 cttgattgat cgcgttagcg atacgagagt tgtttaggac gagccggatc gccttttcgg
     4861 tctcgaacaa aaaattccgg taggtttcat ctatcacggg ttctatatcc ttcacgtaac
     4921 ccaacgagcc gatttccgca cctttgacga tcgcttcgag cacttcttcc acatcgacat
     4981 cgagagtcct caaccgctcc aacagggttt tgatcgtgat tggaatctcg gccacgtggt
     5041 agatactgcc gttgagaaag gcgaacagca aagagtattg gaggtcgagg agcgccagga
     5101 aacggaagcg gtcgccggtt ttcatactcc ccaaaaaggc tttgttgtcg atgagttcga
     5161 aatattttat gctgtccggt agtaggacgt cgggttccgg ggccttcgcc tcggaaaaga
     5221 gtctgctgag atattggtcg atggatacgc gcttcgtcgc gaaggctaag tgtcgggttt
     5281 tttcgaagag gcttctggta tcgcgtaacg gttgggcgac cttaaccaca aggtcgctga
     5341 ccgagatgga atattcctcc gccttcttga acttgatagc attggccaat tgctcctctg
     5401 agagggccgg catctgctcg accgcggtga tcagcttttc tgacggaaag ttgagaacga
     5461 cgaaatcctc ggcgtcgggc gatagggctt ctaccacctt cttgaaatgg gtgacctgga
     5521 tatcagaaag gatctcgtcg gtgaacgagg gatgcgtctt aatgattttc tttagtacct
     5581 tcgccctctt tcccgaaaga tgcaacaaca acgctttcgt gataaacggg ttcgtttcca
     5641 gagctaagac tctacggtta aaaatctgca cagagaatcc ccccaccctc tatggcacct
     5701 taaaaaagcg gataccggcg gtgaccggtg agatggtcaa ccgaaagaag tcgttgcctt
     5761 tagcgacccc cacgctaccg cctttctcta taaccccgaa atgattgaac agaatctgaa
     5821 aatcttgcat aacgcggaat ccgcaaatca ctgtgtcttt acgtatgaaa agcgctttct
     5881 gttttatttc tatcaacgct tcgcagcgaa cgcgagccat ctgccgctcg atggcggcca
     5941 cgaaactctc gggcgttccc accgccgagt gagaaagcga agaggtccaa tgcgtgagga
     6001 acgcggtctc gaccgaaacg gtgatcagga gatagaggat gaactcaact aaagaccagc
     6061 cttttcgtct ctccatacgg ggtccgtcgc tatcgaggaa cccctaaaac ctcgagaggt
     6121 tttgttgccc cgagaacatc gaactgtaca tcgcgtaggc gatcaacccg ataaaaccgc
     6181 ccacgaccac catcatcgcc ggctccagca tagacaccag ctgcttaacg gaagtccgca
     6241 cccgttcctc gaagaaatcg ccgactttat ccaaaacctt gtccagttta ccggttttct
     6301 caccggttcc gaccattgcg atcacgatac ccgggaagag ctcggcttct gtgaaggctt
     6361 gttcgagcgt ctcgcccgtt ttgatgcgtt cggccatttc ctgcgccgat ttttcgaatc
     6421 gataatcgtt cgccgccttg ctggcgagct ccaacgcccg aaggatctcg accccggcgc
     6481 ccaccattac cgacaacgtt cggcagtagc gctccaaaga gctgtttcgt ctgatgttct
     6541 tcaccgccgg aatcacgctc aagatgtagg atagcgcttt ttttccggcg aaggtcttaa
     6601 ggtaataggc taggaaaatc cccaatccga cgacgatgag aagcaatatg agccagttgc
     6661 cgcggaggaa gttgctggcc tgcatgagga tagccattat gccctccgct tctccaccga
     6721 aggcttcgac cagtttgggc aacacgaagg cgaggatggc gatcaccatg atcaccgaaa
     6781 atcccatcac gaaaaccggg taggaggtcg ccgatttgat ctcgtctttc aggcgtttct
     6841 gagactcata aaagctggcg actttttcga gtgtctcggc aagcgtaccg ctcgtttccc
     6901 ctgcttcgat gaggttgacg aagagatcgt caaaggcttt ttcgttgtcg agcgcttcag
     6961 aaaagctcat cccggcttct atattcgaga gaacgctcgt gacgatcttt cggaatttgg
     7021 gcgaaaacac ctcctgctcc gccagggtgg ccaaggcgtt cgccaaattg acaccggctt
     7081 cgatcatggt cgccaactgc cgggaaaagg tcatgatatc cagcaatcgc agcgtcataa
     7141 tccgcttgat ctttttttcc gcgatcgttt ccagagacag gacgatgtat cctctgttga
     7201 tcaatgaggt aaccgctgtg gcttcgttgg ccgagttcac cacaccggtt atacggttgc
     7261 cgctggaatc cgtcgctctg taactgtaat tcggcataat taccacctgt gtccgcgtgc
     7321 tatttgtcta aaccccttcg tttgagctca tccaacccgc gcgggtcgtt tatctttttc
     7381 aaggcctcta ccgcgaattt atagaccgcc atttcgatgc tctttcccgt tttcgggtcc
     7441 acgcttttca ggagggtcgt atcgttgagc aaggcgaaaa gcgggtacac agcccgaacg
     7501 tctttgatct ctccgagcgc gtacgcggcg aaggctttaa tcaccgcgtt cggatccgaa
     7561 agcaaattga tcaggtacag gctggccccc gggtccttta actctcctag cgcgatcacc
     7621 gcttgcatcc gcacgttctc gtcggtgtcc cgtaggaagg cgatcaaata gggcagattc
     7681 cccggggctt tgatacggcc cagggcattg gcgacggtgc ggcggacggc gggattgaag
     7741 gattcggctt ggctatatag gacttctagg gccttttcgc tcccgatcgt gaccaacgtg
     7801 tttagtatgt tgatttttac atcgtctgaa taggtctttt gaaaggcgac catcaaggga
     7861 gcgatcgcct tttccccgat ttgcgataac atagaagtgg ctttggactt gtacccttcg
     7921 ttggttagaa ggttcgtgag gaagttcgcg acttctacga ttccgtccgg gctgttgatg
     7981 tcgataatga tcctggaaac gcgtaaccct tgctcgtagt cctgcataat cccgatcaac
     8041 ttcagtttga cgatcaggtg aggaatggcg agttctccga agttgatcag tgcgctgtcg
     8101 atgcgcacat catcatccga gccgagcatc cacacgatcg gttctagggc caatagactc
     8161 ctgatctgcc ccagcgacca aacggcggac agccggagct ctctattctc gttcttcaaa
     8221 tacgtcagaa gcaaatcctc cacttgtggt tcgtagagat agcccatcgc ttccaaagca
     8281 acccgctttt cggcagtgtt gccgtgattt aggacttgaa gccactcctc gaaagtcaac
     8341 cctttgtaag cgctagccaa aagcgatgtg ccaagaacga agaaaattag gatgtaaagc
     8401 cactttcccc tacgcataaa acccccctca aacgaaatca gataaaaatt gcctttccct
     8461 cgtcacgtta cgtataatcg acctgcattt gcaaaagcac caagccacaa cagagtaacc
     8521 cctcgctggc gcaaatgaac aagatcctct tttttacccc cctgggcgct atgagaatag
     8581 cgcccattcg ctatagccga acgggaaaaa cgcaatagga tggttctatt gtacaacaaa
     8641 cccggtcttt tcgcaacctt atccttttgt taattcttct tttccatcca cctggcttgg
     8701 aacgtcgaac ttaagttcgg catgcctttc caaaaaccaa agccgtttcg cacagagaac
     8761 acggagtaca cagagagaac acgagcgtct cctatgagcg cttgggaacg ccaaactttg
     8821 gtttggtatg tctttccaaa aaccaaagcc gtttcgcaca gagaacacgg agtacacaaa
     8881 gagaatacga gtgtctctta tgggcgcttg ggaacgccaa actttatttt ggcatgtctt
     8941 tgcctttctg ggaacacctg cggggcgcca ccagagaggc ggactttagt ctggcacgtt
     9001 tttgctttta ccttgtgctc cgtgccctct gtgttctccg tgtgagcctc tgtttttccc
     9061 taaaaaacac gtgccaatct aaagattggc gttctcaggg cactctcggc cgttcggcgt
     9121 tcccagatgc accttatacc atatatagat atagacggca ttccgttgag tttcctccca
     9181 agagacggac tcagggttga gtcagcctcg gagcgcggcg tgacaacagg gttatagcgg
     9241 gctgcgagcc ggtctcgggt ggtcaagtat gagacctcct cccagtcttc gctatcgctt
     9301 tttgtccatc gcggaatacg ccgcttgaac gatatccgca gccgtcatat gatacttttc
     9361 catgaggaag tcggattttc ccacttctcc gaaatggtcc tgaaccccga tccgaaagac
     9421 tggcaccgga cagtgctccg agaccacttc cgcgaccgcg gaaccccaag cgttcagcac
     9481 gctgtggttt tcggccacga cgatcgcccc cgtctcgcgc gcggcctgga taatggttgt
     9541 agcgtccacc ggcttgagcg tatgcatatt taatacgcga gggcgcacac cctcttttcg
     9601 caatatctcc gaggctttca aggattgctt caccatcatc ccactgcaaa tcagcgtcac
     9661 atcctccccc gtttccaggg tttctatctt gcctattgtg aaggatcgat cgggtgagaa
     9721 gaggacttct ttattcttgc ggtgcagccg catatacacc ggccccgggt gcgagatgac
     9781 ctggggaagc aaggccgcca attgcacgga atcgacgggc tcgacgatcg tcataccggg
     9841 aaggttccgc atgatccccg catcctcgaa gctcatatgc gtgccgccgt tgagctccgc
     9901 gctcacgccg gggtccgcgc caccgatttt gacgggtaac cgggagtagg cgatcgatat
     9961 cgtcacctga tcgaaggctc tgcgcgtggc aaatgccgtg aaggaatgcg cgaaggggat
    10021 catccccatc accgccaaac ccgcagatat gcccatcagg ttcgcctccg cgacgccgac
    10081 gttaatgagg cgatcgggaa atcgttcttt gaaggctttc gttccggtag aactcatcaa
    10141 gtccgcctct aacaggacga tccggtcatc cttttgcgca tgctctatca atagctccgc
    10201 gtaaaccttt ctcatttcca atccttcgta catccttatc tcccctcgcc ggaggtcacg
    10261 ggctcgcctt cttgtcgcaa gcgctccact gcctcacgcc actgttcgtc cgttaccacc
    10321 atattgtggg agctcacctt cccttctgaa aagaaagcgc cttttccctt cgtcgtgttc
    10381 aggatgatca tcgtcggtct gcctttaaac cgcttcccga gagtcaaggc gctatcgatc
    10441 gcgacgatgt cgtggccatc caccgagtag acgttccacc cgaaggccct ccatttatcc
    10501 gccaccggtt ccaaagaatt gatctcggag actagcccgt ctatttgcat attgttgtag
    10561 tcgagaaacg cgatcaggtt atcgagaccg tggttgcccg cgaacatcgc ggcttcccaa
    10621 atctggcctt cttggctttc cccgtctccg ataagcgcgt agacccgtac gccctgaccc
    10681 tgcatccgcg cggccaacgc cattcccacg gcggctgaaa acccttgagc taaagacccg
    10741 gctgtcatat cgatcccggg cgttttcaac cggtcacagt ggctggggag attggtgttc
    10801 ggcctgttca gcgtatccag ccagctcctc gggaaatacc ctttatcggc taagaccgcg
    10861 tacagtccgg ggccgctatg acctttcgat aacacgaagc gatcacgttt ttcccataac
    10921 gggttttgcg gatcgacggt catatgcttg tagtagagca cggtgagcgc ctccacgatg
    10981 gaaagggaac caccgatatg cccgaccccc agtttgccga tcatctccaa tatcaaccgg
    11041 cggatttcgt ttgccttcga caccaaagcg tttgtttgcg tagcgtccat tgacatcatc
    11101 acctcacata gtctatttcg atttccttga ttatataacg aaagccactt tttttgaatt
    11161 cttcgagcgt gcccttccgg gatcgcatcc ctgagagcgt cctgggaacg ctctgggaac
    11221 gccaatttta gtacgtccag ataaggcgga aatgttcagc aggaagaaag caagcctgcc
    11281 ggggaaaaag tcagaa
//
LOCUS       DAOPXN010000068        10620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6305_length_10620_cov_4.548982, whole genome shotgun sequence.
ACCESSION   DAOPXN010000068 DAOPXN010000000
VERSION     DAOPXN010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10620
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6305_length_10620_cov_4.548982"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..1131)
                     /locus_tag="PKM49_05255"
     CDS             complement(142..1131)
                     /locus_tag="PKM49_05255"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNY82254.1"
                     /translation="MVLVTGATGHIGNVLVKYLVASGEKVRALYAPGDDPSPLKGLQV
                     EGREIDILDYEALRAAITGVDIVFHLAGLISILPFHQKRVYDINVGGTENIIRACKET
                     GVRKLLYVSSIHAFEDKKRTLKMDERNAIDPAKTSGSYGKSKAIATLKVEEAVRDGLN
                     AVIVCPTGVIGPYDYKLSELGRLFIDVAKGSMPFSVKGAFDFVDVQDVAQGILLAAKQ
                     GRTGERYILGGTQIEINELIGLIEKAVGTCRKRVVVPTGLAYFASFFTGLHALIWKKK
                     PRFTTYSISTVSMWNTYSHEKASREIDYRPRPIEKTVRETLDWLRQEGHLSSR"
     gene            complement(1226..2062)
                     /locus_tag="PKM49_05260"
     CDS             complement(1226..2062)
                     /locus_tag="PKM49_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HNY82255.1"
                     /translation="METLHVVYPETEQAFIESLEERSAILNGGTDIHVKIRAGKWPYT
                     RLVSLERVETLKGISQEGKYIRIRCRTTFSDLLANPLIAQHFPLLYQAAYEVGSNQIR
                     NRGTLVGNIVNASPAGDGILALSVYDAWLEIWSRKGQKRLAQIHEFILSPGKTVLKAD
                     EFVHSVILQKPTKPLRALFYKVGQRKAMAIAIASMGVLLETKHDRIKEIRIAFGSVAP
                     TVVRIPEIEERAFDQTLNEATVESWMPFLEKHVVPIDDIRSTAKYRKEVCQRLLYKIL
                     DL"
     gene            2175..2522
                     /locus_tag="PKM49_05265"
     CDS             2175..2522
                     /locus_tag="PKM49_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HNY82256.1"
                     /translation="MGFEGYYEIKKDQELAGFLGIAAKTKKIVVGKDHIKSYLKTPKI
                     TKKLLILACDISEGQKKDWMHRGSYAGAKILYLCHTPKTLLAKAIGKTEVSAVSIADD
                     RLIDGLVAKLGLS"
     gene            2522..3406
                     /locus_tag="PKM49_05270"
     CDS             2522..3406
                     /locus_tag="PKM49_05270"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HNY82257.1"
                     /translation="MYGYLRPLKSPHHDYTLFRAHYCCLCKTIQSEYGLPFRAGLSYE
                     MAFFALLLNALQGADVSIRRETCLIHPFRKQAVFKSSPTMSLCAHLNMALLAGKLSDD
                     IADEERFRPVRRIGLRYLESRAGNVFKGYSRLVSDAYESIRLLEGGKVDDVDAISALF
                     GELLAALFLEVSAGSGLEPPEPMIHFSRVLGRWVYCIDALDDLQNDFRAKRYNPLIYR
                     YKETLLSSDSLEKAYESIGSQEAVRLRLLSEHIMSEYRLFRMRLGLYSVEIDDIIFRT
                     IPCACARIAKKNVVKEKK"
     gene            3403..4029
                     /locus_tag="PKM49_05275"
     CDS             3403..4029
                     /locus_tag="PKM49_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNY82258.1"
                     /translation="MSQNPYKVLGVEETASPEEIKKAYTDMVKKYHPDRFKEDYMKEL
                     AEQKMKEVNEAYDAIQKGNAYYDRSRSEEPPRYSTDRSARSESIGTLRDVRMYIHNRS
                     FSKAREILQSISLRTAEWYYLMGIVEVNYGNYDQGKYYINQAVTMDPGNAEYREAYQQ
                     LFAGTIFSHNANPYRQHDPAQACCQVCLAAWCLDACCECGGGDFITCC"
     gene            4832..5176
                     /locus_tag="PKM49_05280"
     CDS             4832..5176
                     /locus_tag="PKM49_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82259.1"
                     /translation="MHLGSFVLPKERRKELAGCLERKLFGQSTFQEEAPDIEHIATLI
                     TEQYGRFERRREEKRSRQEQQEIVPVDTHTRSLGGEVVAHRASKALGLNRILKEAQMS
                     ARECALAEATVV"
     gene            5342..5674
                     /locus_tag="PKM49_05285"
     CDS             5342..5674
                     /locus_tag="PKM49_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82260.1"
                     /translation="MTSETTPGGKEAQALRRTDAGALVYFDIGVSSAERIEQRLRKTG
                     DHRRWSTIRKTLSTHQRCTAVFRDEGDKVYHLRVSGKPESLHEDIYKELGVPMLKNRQ
                     LTEVARRL"
     gene            complement(6043..6351)
                     /locus_tag="PKM49_05290"
     CDS             complement(6043..6351)
                     /locus_tag="PKM49_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNY82261.1"
                     /translation="MYGLDQTVINDLRRVFSKYPSIEKVILYGSRAMGTYRRGSDIDI
                     ALFGENLSNQTIFDIQEEIEALYLPYSFDISIWERIENEDLLDHIRRVGKVFYERAET
                     "
     gene            complement(6352..6774)
                     /locus_tag="PKM49_05295"
     CDS             complement(6352..6774)
                     /locus_tag="PKM49_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009834352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HNY82262.1"
                     /translation="MMKDIRWIQRYSNFIKALSNLNEAVELANERDLSKLEKQGLIQS
                     FEYTFELAWNTIRDFYRSKGEVDIQGSRDAFRLAYERGLIEEGQVFMNMIKSRQLSSH
                     TFNEETANEIYQDIVNQYFSAFKCLSERLKQEKRKEEN"
     gene            7384..8679
                     /gene="asnS"
                     /locus_tag="PKM49_05300"
     CDS             7384..8679
                     /gene="asnS"
                     /locus_tag="PKM49_05300"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNY82263.1"
                     /translation="MWVYIEDFKNHVGETVILKGWVFNKRSSGKIHFLQLRDGTGTTQ
                     GIMEKAKVEESLFEKAKGLRIESSVEVEGVVREDARSKGGYELDVTGLRILQIPVEEY
                     PITKKEHGTEFLMEQRHLWLRSTKQYHIMKVRNEVIRATRAFYDEQGFTLIDTPIFTG
                     AIGEGAGNLFQLDYFDYGKAYLTQTGQLYLEAACMSFGKVYNLGPTFRAEKSKTRRHL
                     TEFWMNEAEVAYYDFQDNMRLQEELISYIVHRVLENCSESLAILERDTTKLAGIKPPF
                     PRLSYDEAVKMLQDKGHPIRWGDDLGAQDEAEISNQFEKPVMVYKYPTQVKAFYMQPD
                     PQRPEVVLCDDMLAPEGYGEIIGASERIHDYDLLVRRIEENGLPLDSYDWYLDLRKYG
                     SVPHSGFGMGIERTVCWICGIHHIREAIPFARTLYRIKP"
     gene            8696..9346
                     /locus_tag="PKM49_05305"
     CDS             8696..9346
                     /locus_tag="PKM49_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY82264.1"
                     /translation="MEERIARFLIADDEYKVRDLIRKYVNACGHEAECVSNGEEVLRK
                     VYGHNPYDGIFTDLRMPGLDGLSLIRALYQEQIRIPIVIVSAFGSVEAIVEAFKKGAL
                     DYISKPFSPEEIRDAIKTMLNQVVDLKISSSTVKGLIEQKAYPGALKYLLPLFSHFPS
                     SPIPHYLYGLLREAQGDFDEAFRHFSASLALDKGYEPSAKKREEYRPKHLKEGKDE"
     gene            9339..9773
                     /locus_tag="PKM49_05310"
     CDS             9339..9773
                     /locus_tag="PKM49_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNY82265.1"
                     /translation="MSKRKSWYAILIGSGRFGARLAGKLSNEDHSVVVIDKNESKFEN
                     LPLEYTGFTIQGDATDIEVLKAAKVQKADLFLALTDDDNTNFFVSQVAKVIFHVPHVI
                     SRVYDPANLPLFHEYKIETITPVLLALSTANRMLEKIAEEEK"
     gene            9770..10432
                     /locus_tag="PKM49_05315"
     CDS             9770..10432
                     /locus_tag="PKM49_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNY82266.1"
                     /translation="MKVVLAGGKKITYYLAKNLISKGMAVTIINKDKAFCEEMAKTLK
                     ALIIHGDASNLSVLKKVEIFDNDLVVALTNRDQDNLIICELAARVLKVNQVFSLINDS
                     ENEQIFRELGITNTINMSEIFTGVIEQRLASYPIINLMPFEEGKALVIQIEVEEGFPI
                     VGKWLKDLPFAEGALVGTVNRKGNVFIPHGDTVFEVGDRLLVICLPVDQTQTLQAITG
                     WD"
BASE COUNT         2656 a         2590 c         2788 g         2586 t
ORIGIN      
        1 gtctgggtac ctggtaggcg ccacgagcga gcgcttcgga atacgggcag ggttcttctc
       61 gatcttcatc tatttcgtgt gtacgttcgt ttgctccgtc attctttatc gtatgactcg
      121 aaagagggaa tagccggccg cttaccttga agacagatgc ccttcctggc gcaaccagtc
      181 cagcgtttct cgaacggttt tctcgatcgg tctaggtcga tagtctatct ctcgggaggc
      241 tttttcgtga gagtatgtgt tccacataga aaccgttgaa atggaatagg tcgtaaaacg
      301 gggtttcttt ttccatatga gagcgtggag ccccgtgaag aaagaagcga aataggcgag
      361 tccggtcggc accacgacgc gctttcggca agttccgact gccttctcga tgagcccgat
      421 cagttcgtta atctcgatct gtgttccgcc gaggatgtac cgttcaccag tccttccctg
      481 ttttgcggcc agtagaattc cctgcgccac atcctgcacg tccacgaagt cgaaggcgcc
      541 tttcacgctg aagggcatcg atcccttcgc cacgtcgatg aacaaacgcc ctagctccga
      601 cagcttgtag tcgtagggcc cgatcacgcc ggtggggcag acaatcacgg cgttcaagcc
      661 atctcgaact gcctcttcta ccttaagagt cgctattgcc ttagacttgc cgtaggagcc
      721 ggaggtcttg gccgggtcga tagcgtttcg ttcatccatt tttaacgtcc tctttttgtc
      781 ttcgaaggcg tgaatggaac tcacgtagag gagtttccgg acacccgtct ctttgcaggc
      841 gcggatgatg ttttccgtac cgccgacgtt gatgtcgtag actcgcttct gatggaaggg
      901 gaggatagag atcaaacccg ccaaatggaa aacgatgtcg actcctgtga ttgcggctcg
      961 aagcgcctca tagtccagaa tatcaatctc tctaccctct acctgcaatc ctttcagcgg
     1021 ggagggatcg tcaccgggag cgtataaggc ccgcaccttc tccccggatg ccacgagata
     1081 tttcaccaac acattcccta tgtgcccggt cgccccggtc accaatacca taataacccc
     1141 tccaaatgta agatagacgg attataccac aatagtacaa atattcaact atacaatata
     1201 tgtattattc gtctcgcggg agggctcaga gatcgagaat tttgtatagc aggcgctggc
     1261 aaacctcttt tcgatatttg gcggtcgatc ggatatcgtc gatcggcacg acgtgctttt
     1321 caagaaacgg catccaactc tccaccgtgg cttcgttcaa cgtttggtcg aaggcccgtt
     1381 cctctatttc ggggattctc accacggtcg gggcgaccga gccgaaggcg atccgtattt
     1441 ctttgatcct atcgtgtttc gtttccaaca ggactcccat actcgcaatc gcgatcgcca
     1501 ttgccttcct ctggccgact ttgtagaaaa gcgctcgaag gggtttcgtc ggtttctgta
     1561 aaataacgga gtgaacgaat tcgtcggctt tcagcacggt ctttccgggt gagaggatga
     1621 attcatgaat ctgagccaag cgtttttgcc ctttccgcga ccatatttct agccacgcgt
     1681 cgtagaccga gagcgcgaga atgccgtctc cggccggcga ggcgttgacg atattgccga
     1741 cgagggttcc acggttacgg atctgattgg atccgacctc gtaggcggcc tggtatagaa
     1801 gcgggaaatg ctgcgcgatc agcggattgg cgagcagatc ggaaaaggtt gtgcggcaac
     1861 ggatacggat gtatttacct tcttgcgaaa tacccttcag ggtttctacc cgctccaagc
     1921 tgaccaatcg ggtgtacggc catttcccag cgcgtatttt cacgtggata tcggtgcccc
     1981 cgttcaggat cgcagagcgc tcctcgagtg attctatgaa cgcctgctct gtttccgggt
     2041 agacgacgtg taaagtttcc atattttcat cccctggcag atgacctatg atgaagatta
     2101 taccaaaaaa acgctggaaa tcgtcgcatt tatggtaaga ttcctggaag gagcgggata
     2161 tcgaggagaa gagaatggga tttgagggtt actacgaaat aaaaaaagac caggaactgg
     2221 ccggattttt gggtatcgcg gccaaaacga aaaagatcgt cgtcggaaaa gaccatatca
     2281 agagctatct gaaaacgccg aagatcacga aaaagctctt gattttggcg tgtgacatca
     2341 gcgaaggtca aaaaaaggat tggatgcatc gcggttccta tgcgggcgca aagatccttt
     2401 acctgtgcca taccccgaaa acgcttctgg cgaaggcgat cggaaaaacc gaggtcagcg
     2461 ccgtatcgat tgcggatgat cgactgattg acggtctagt ggcgaaactc ggcttatcgt
     2521 aatgtacggg tatctccgcc cgttgaagag cccgcatcac gattacaccc tctttcgcgc
     2581 gcactattgt tgcttgtgca agactattca atcggagtac ggtttgccct ttcgcgcggg
     2641 gttatcctat gagatggcgt ttttcgcgct cctcctcaat gcgcttcaag gcgccgatgt
     2701 gagtatccga cgtgaaacct gtctgataca cccgtttcgt aaacaggcgg tcttcaaatc
     2761 gagccccacg atgagcctgt gcgcgcatct gaatatggcg ctgctcgccg gaaaattgtc
     2821 ggacgatata gccgacgaag agcgtttcag accggtgcgc agaatcggtt tgcggtatct
     2881 ggagagccgt gccggaaacg tgttcaaagg gtattcgcgg ttagtgagcg atgcctatga
     2941 atcgattcgg ctcctcgaag ggggaaaggt cgacgacgtg gacgccatct ccgccctctt
     3001 tggagagcta ctcgcggcac tttttttgga ggtgagcgcg gggagtgggc tcgagcctcc
     3061 ggaaccgatg atacatttca gtcgggtttt gggacggtgg gtatactgta tcgacgcgct
     3121 cgatgacctg cagaacgact tcagggccaa gcgctacaac ccgctgattt atagatacaa
     3181 agagaccctg ttgtcgtccg attccctgga gaaggcatac gaatcgatcg gttctcaaga
     3241 ggccgttaga ctgcgtttgc tatcggagca tataatgagc gagtatcgcc tttttcggat
     3301 gcggttgggc ctctattccg tggagatcga tgatataata ttccgtacga tcccctgcgc
     3361 gtgcgctcgt atcgccaaaa aaaacgtcgt gaaggagaag aaatgagcca aaacccgtac
     3421 aaagtgttgg gagtggagga gactgccagc cctgaggaaa taaaaaaagc ctacacggat
     3481 atggtgaaga agtatcaccc cgaccgtttt aaagaagatt atatgaagga attggcggaa
     3541 cagaagatga aagaggttaa cgaagcctac gacgccatcc aaaaagggaa cgcgtattat
     3601 gatcgatcaa gaagtgaaga gcctccccgt tattcgaccg atcgatctgc tcgctcggag
     3661 tctatcggca cgttacgcga cgtcaggatg tatatccaca atcggagttt tagcaaagcg
     3721 agggagatct tacaatctat ctcactaaga accgcggagt ggtactatct gatggggatc
     3781 gtcgaggtga actacgggaa ctacgatcaa ggtaagtact atattaacca agcggtgacg
     3841 atggaccctg gaaacgcaga gtaccgtgaa gcttatcaac agctcttcgc cggcaccatt
     3901 ttttctcaca acgcgaaccc ctaccggcag catgacccgg cgcaagcttg ttgtcaggtg
     3961 tgtttagccg cttggtgttt ggacgcttgc tgcgaatgtg gcggcgggga tttcatcacc
     4021 tgctgctgac ctcctcgggg aatgccttct gggtgctgag cttctgtgcg acatcgatcc
     4081 catgcgaagc atcgcggcgt aagcgctctt ttttctcgcg acggcgcgat actctttttt
     4141 actttcgatc tatcggtttc taatacgttt tgtttgaata tggagaaaga acggatcgcg
     4201 gggtcccttc ggttaccccg cgataaccta tgaagttcgg cgttcccaga gcttctctct
     4261 gtgttctttg cgtactctgt gcgaaaccat ctttcgcttt tgaaaagata tgccagactg
     4321 aagtccggcg ttcccagggc gctcctgggg cggcgtaaaa aagacaaaag gcggatcgcg
     4381 agacttcgtg ggtttccgaa gtcagcccgc tggttacccc acgataacac gcacgagcat
     4441 cgcggcgtaa gagtttatgg gctctctaga gcttactgaa aagcccaaaa acaggccgaa
     4501 ctaaagttcg gcattcccag aaaagcgttt ccagtggcgc tttcaaaaaa cgtgccgaac
     4561 taaacccgac tttcgaccgg ttttgcaaaa aaagaaaaaa caacgctgaa acccacacga
     4621 aatcgttgag aattttgggt tttcagaatg tagcggccta atagcgtaac gatatcgggc
     4681 ataaatggaa acgattcgct tttcgtggca ccctatccct atgttcatac gtcagtcaag
     4741 aactcgaaga acctccaatg gaaagatata taccaaatat gtcctcgtga aatccgtgcg
     4801 aacagagaaa ggacctcggc agagaacgat tatgcatctg ggttcttttg ttttaccgaa
     4861 agaacgaagg aaagaactgg cgggatgtct ggaaagaaaa cttttcggac agagtacctt
     4921 tcaagaggaa gcccctgata ttgaacacat cgcgacgcta atcacggaac aatacgggcg
     4981 gtttgaacga agacgggaag agaagcgatc tcgccaggaa cagcaagaga tcgtaccggt
     5041 ggatacgcac acgcgttctt tgggaggaga ggtggtagcg caccgggcgt cgaaggcgtt
     5101 gggactgaac aggatactga aagaggcaca gatgagtgcg cgggagtgcg cgctggcgga
     5161 agcgacggtg gtctgacgcc tacttgatgg aagagaaggg atacgcgtat acggtggtat
     5221 aacgacgaga gcgacggttt ttcgaagcgc tgaagaggtt atctggaaag atacgggtga
     5281 aacggataaa aagatagaaa aagtccggga acggtatccc tccattacgc agtactatac
     5341 agtgacgagc gagacgaccc ctggcggaaa agaagcccaa gccctgcgta ggacagatgc
     5401 aggcgcactt gtttatttcg atattggcgt atcgtctgct gaacggatcg agcaacgatt
     5461 gaggaaaacg ggagaccatc gacgatggtc gaccatccgt aaaacgctct cgacccatca
     5521 acggtgtacg gcggtctttc gggacgaagg ggataaggtg taccatttga gggtatcggg
     5581 aaaaccggaa tcgcttcacg aggatatcta caaagagctg ggcgttccaa tgctgaagaa
     5641 ccggcaactg actgaggtag ctcgtcgatt gtagtggccg gcttttttga gaaagtggct
     5701 tatatcgccg tttgtagcac ttttagtcga aagttgggct aagggttaag acactctcct
     5761 acaatccgtt ggcttcactt cccgaaatga tgagaaatca ttctctcttt tcagtgtgat
     5821 cgtggtatgt cccactcata gggcgggcct tctctccaca taggccaaat gattagccct
     5881 tgtgttttgc gaatcatccc tgatttggaa tgttttattc ttcctttacg gatgtttcgt
     5941 ttttccttgt tcgaggaagt cggtgttacc ggaagataag acagatagtg ttattgggtg
     6001 agaggtgaaa ggctcgccct tcgtacctga ttcgtcaaca gcctacgtct ccgctctttc
     6061 ataaaacacc ttaccgactc ttctgatgtg atcaagtaaa tcctcatttt cgatgcgttc
     6121 ccatatcgaa atatcaaaag aatacggaag ataaagcgct tctatctctt cttgtatatc
     6181 aaaaatcgtt tgattggaaa ggttttctcc gaacagtgcg atatcgatat ccgagccgcg
     6241 acgatacgtg cccatcgcgc gcgaaccgta aagaatcacc ttttcgatag aaggatattt
     6301 agaaaaaacg cgtctcaaat cgttgatgac cgtttgatcc aatccgtaca ttcaattttc
     6361 ctctttacgt ttttcttgtt tcaaccgctc agaaagacat ttaaaagcgc tgaaatattg
     6421 gttcactata tcttggtaga tttcgttcgc cgtttcttcg ttaaaggtat ggcttgacaa
     6481 ttgcctgctc tttatcatgt tcataaagac ttgtccctct tcgatcaaac ctctttcgta
     6541 cgcaaggcga aaggcgtcgc gactaccctg aatatcgacc tccccttttg aacggtaaaa
     6601 atctcttatc gtattccaag ctaattcaaa cgtatactcg aaagactgga tcagcccttg
     6661 cttttctaat ttcgacaaat ctctttcatt cgctaattct acggcttcgt tcaaattgga
     6721 caatgctttt ataaaattcg aataccgctg aatccaccgg atatccttca tcattccccc
     6781 tttttttctt ggagaacctt ggccaaatat cgcgccttcg tccctaacga tatagtatag
     6841 caataaacgc gtctcatagg atcgattatg aaaaacgata ctcccaactt tcctattggc
     6901 ttgcacaaag taatgcgtaa aaaatggtat gggtgctttg ttttcgcccg gctctccttg
     6961 cgctctgtgt gaactcattt tttcctttca aaatccgaaa aaagtagaac ctgccggact
     7021 gaagtccggc gctcccagga aacatgctta cttgaaagtt ggcgttccca gggcgtttcc
     7081 aagcataaca tcgcagtttg agcgctcagt gcataggggg atcgcggggt cccttcggtt
     7141 accccgcgat aacctatgaa gctcggcgtt cccagagctt ttctctgtgt tctttgcgta
     7201 ctctgtgcga aaccgtcttt tgcttttgaa aagacatgcc agcctgaagt tcagcgttcc
     7261 cggcccggcg gtcccagaaa acataagccc aaccagagtt cggtgctcac aagctggcac
     7321 ttcctaaaaa ccgcagtttt tttatgttat aattgccgat gattttcttt ctgggagggc
     7381 tttatgtggg tctatatcga ggattttaaa aaccacgttg gagagacggt tatcttgaaa
     7441 ggttgggtgt tcaataagcg ctcgagtggg aagatacatt ttttgcagct cagagacgga
     7501 acagggacga ctcagggaat aatggagaaa gccaaagtcg aagagtccct tttcgaaaaa
     7561 gccaaagggc tccgtatcga gagtagcgtc gaagtggaag gggtcgtccg agaggatgcc
     7621 cgttcaaagg gcggctatga attggatgtg acggggttga ggattttgca aatcccggta
     7681 gaggagtacc cgataacgaa aaaggaacac ggtactgaat ttttaatgga acagcgtcac
     7741 ctgtggctcc gttcgaccaa acaataccat attatgaagg taaggaacga ggttattcgc
     7801 gcgacgaggg ccttctatga tgaacaggga ttcactctga tcgacacgcc catcttcacc
     7861 ggagcgatcg gcgaaggcgc gggtaatctt ttccaattag actatttcga ttatgggaag
     7921 gcctacctca cccagaccgg gcaactctac ctggaagcgg cttgtatgtc tttcgggaag
     7981 gtttacaacc tgggtcccac cttccgcgcg gaaaagtcga agacccgcag acacctgaca
     8041 gagttctgga tgaatgaagc cgaggtcgca tactacgact ttcaggataa tatgcgctta
     8101 caagaagagc tcatctctta tatcgtgcac cgcgttttgg agaattgctc tgaaagcctc
     8161 gcaattctgg agcgtgacac cacgaaattg gccgggatca agccgccctt cccacggttg
     8221 agttacgatg aggccgtgaa aatgctccag gacaagggac acccgattcg atggggggac
     8281 gatctgggag ctcaggacga ggcggagatt tcgaaccagt tcgaaaaacc ggtgatggtc
     8341 tacaaatacc ccacccaggt gaaggccttc tatatgcagc ctgaccctca aagacccgag
     8401 gtggtcctgt gcgacgatat gttggccccc gaagggtacg gagagatcat cggggcttcc
     8461 gagagaatac acgattacga tctactggtc aggcggatag aagaaaacgg gttacccctg
     8521 gatagttacg attggtacct cgatttacgt aagtacgggt cggtgccgca cagcggtttc
     8581 gggatgggca tcgagcggac cgtgtgctgg atatgcggta tccatcatat ccgcgaggcg
     8641 atcccgttcg cgagaacgct gtaccgcatc aaaccctaag ggcggatagg agagaatgga
     8701 ggaacgaatc gcgcggtttt tgatcgcgga cgacgaatat aaggtccgcg acctgatacg
     8761 caagtacgtc aacgcctgcg gacacgaagc ggagtgcgtt tccaacggag aggaggtcct
     8821 ccgcaaggta tatggccaca atccttacga cgggatattc accgaccttc gtatgcccgg
     8881 attggatggg ctttcgttga tacgcgccct ctaccaagag caaatacgca ttccgatcgt
     8941 catcgtctcc gcgttcggga gtgtggaagc gattgtggag gccttcaaaa agggcgcgtt
     9001 ggattacatc tcaaaaccct tttcacccga ggagatccgc gacgcgataa aaacgatgct
     9061 caatcaagtg gtcgacctga aaatttcgtc tagcacggtc aaggggctaa tcgaacagaa
     9121 ggcttatccg ggggcgttaa agtacctttt gccgctcttt agccatttcc cctcttctcc
     9181 gatcccgcac tacctctacg ggttgctacg agaagcccag ggcgattttg acgaagcctt
     9241 tcggcatttc tccgcttcct tggccttgga caaagggtac gagccttcgg ccaaaaaacg
     9301 ggaggagtac cgtccgaagc acctgaaaga gggaaaagat gagtaagcga aagagctggt
     9361 acgcgatcct gatcggttcg ggacgcttcg gcgcgcggtt agcgggcaaa ctctccaatg
     9421 aagaccatag cgtcgtggtg atcgacaaaa acgagagcaa gttcgaaaat cttccgctgg
     9481 aatacacggg cttcacgata cagggagacg ccacagacat agaagtcctg aaagctgcga
     9541 aggtacagaa ggccgatctc tttttggcgt taaccgacga tgacaacacg aactttttcg
     9601 tgtcccaagt ggcgaaagtg atctttcacg taccccatgt catctctcgt gtgtatgatc
     9661 cggccaacct cccgttattc cacgagtaca agatcgaaac gatcacccct gttttgttag
     9721 cgttgagcac ggcgaacagg atgctcgaaa agatcgcgga ggaagaaaaa tgaaggtcgt
     9781 gttggccggt ggaaagaaga tcacctacta cctggcgaaa aacttgattt cgaaagggat
     9841 ggccgtgaca atcatcaata aagacaaggc cttttgtgag gagatggcca aaacgctcaa
     9901 agccttgatc atccacgggg acgcctccaa tctgtcggtg ctaaaaaagg tcgagatctt
     9961 cgacaacgac ctcgtcgtcg ccttgacgaa cagagatcag gacaacctca tcatctgcga
    10021 gcttgccgcg cgggtcctga aagtcaacca ggtcttttcg ctcatcaacg actcggagaa
    10081 cgaacagatt ttcagggaac tcgggatcac aaacacgatc aatatgagcg agatctttac
    10141 cggggtgatc gagcaacgtt tagcttccta cccgatcatc aaccttatgc ccttcgagga
    10201 gggcaaggcg ctcgtgatac agatcgaagt ggaggaagga tttcccatcg ttggaaaatg
    10261 gcttaaggat ttgcctttcg cggaaggagc tttggtcgga accgtcaaca gaaagggaaa
    10321 cgtctttatc ccgcacggag acaccgtttt cgaggtcgga gatcgactcc ttgtgatctg
    10381 cctacctgtg gatcaaaccc aaacgttgca ggcgatcacc ggttgggatt gatgggcgag
    10441 gatgcatagc cgctcggact tgacgattca ataccgcacc atcctgatga atatcggctg
    10501 gttgtgtatc gcttccagct ttcttttttt gactcccctg accgctttgg tgttctaccc
    10561 gcaagaggct cattatgcgc tttgtttcgt acaggccggt tttatctctt ttaccttcgg
//
LOCUS       DAOPXN010000069        10289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6557_length_10289_cov_7.228845, whole genome shotgun sequence.
ACCESSION   DAOPXN010000069 DAOPXN010000000
VERSION     DAOPXN010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10289
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6557_length_10289_cov_7.228845"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PKM49_05320"
     CDS             complement(<1..352)
                     /locus_tag="PKM49_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003412681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HNY82267.1"
                     /translation="MKYAWLDEYCLSKLGTTKDFKEEWEATRYMLRGKMYAMVGENKE
                     KQPIITLKLEPMRGIALRETYADIAPGYYMNKVHWNSINLRGNVPDAVLKDMIDESYR
                     LLLASFSQKAQKEIT"
     gene            complement(380..1255)
                     /locus_tag="PKM49_05325"
     CDS             complement(380..1255)
                     /locus_tag="PKM49_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82268.1"
                     /translation="MKKSEKKRTEAFDSSEELRRTFRKIRRHHGFIVGFLLLFSLCLL
                     TATSLLFTPHDPYKMNPKASFQPPSWNHPFGTDDFGRDTFSRCMVGSQTALLVGLVST
                     SISLGFGLLLGLLAGLSSKWVDEIIMRLMDGLYAFPAILFAIALISVLGSNVKNATIA
                     IGIARIPIFAREVRNSVRQVRIKNFAVAARAMGVSYPRQILFYYLPSILAPLTVLTSS
                     SFAYTILSESGLSYLGLGSQPPFPSWGRLLMESQRFLTRAPWLSVFPGIMIAWAVLAF
                     TLLGDGLRDIADPKI"
     gene            complement(1252..2196)
                     /locus_tag="PKM49_05330"
     CDS             complement(1252..2196)
                     /locus_tag="PKM49_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82269.1"
                     /translation="MLLVLFGKRLITGFITILAVALLTFFIFNLLPGDPALVMLGLDA
                     DSEVLSALRKELNLEAPAILRFGHWIADLASGDFGRSITYRKPVTQVIVESLPPTLSI
                     TIVGSALTLVLSLFLGILAFKRHNRWFDYALTLGSQLGIVIPSFWMGILLLFVFTLKL
                     EWFPFGQYAPLRTGFFSWLRTIFLPSLSVSLVAISIMMRMVRSSLLENAREDFVRTAK
                     SKGVPERKIVYKHILKNALGPIVTLFGLQISSILAGTIVIENVFSIPGIGRLLLLAVQ
                     RRDLPVVQGVVLWIATMTVLINIVTDFVHMLLDPRVSL"
     gene            complement(2227..2769)
                     /gene="hslV"
                     /locus_tag="PKM49_05335"
     CDS             complement(2227..2769)
                     /gene="hslV"
                     /locus_tag="PKM49_05335"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNY82270.1"
                     /translation="MEAMIGTTVLVVHRGKRTVIGSDGQVTLGQSVLKAKARKVRKLG
                     DGKVIAGFAGSVADSFTLFEKFEEKYKKHSQQLQRAAVELAKEWRTDRILRHLEAFLI
                     VADGETILVISGNGEVIQPDEELVAIGSGAGFALASAKALYRNTDLDAKEIVERSLQI
                     ASEICIYTNQNFSFEEIVVP"
     gene            complement(3603..3749)
                     /locus_tag="PKM49_05340"
     CDS             complement(3603..3749)
                     /locus_tag="PKM49_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82271.1"
                     /translation="MLFKVEVNEEGRIKSYTIEANNRKEAKLKVKKARKKPVIGVQVL
                     QKAI"
     gene            4107..4601
                     /locus_tag="PKM49_05345"
     CDS             4107..4601
                     /locus_tag="PKM49_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HNY82272.1"
                     /translation="MLNPKMEMELNKQINEELYSAYLYQSMAAYFAANNLNGFAKWMD
                     VQAAEEVNHARKFYHFIIDRMGRVVLEAIEKPPLEWDSPLAAMEAALKHEQHVTGRIH
                     FLVDLAREVKDKATEVMLNWFVMEQVEEEASVDEYVQMLKMFGGKPQGLMMINREMGK
                     REDD"
     gene            complement(4682..5761)
                     /locus_tag="PKM49_05350"
     CDS             complement(4682..5761)
                     /locus_tag="PKM49_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY82273.1"
                     /translation="MRIQAALIGCGRIGSNKHLEAFLANRERLDLIAVCDLKPERAER
                     VADAYATRAGKRPVVFTDYRKTLSTGIEMAAIATESGNHYTIAMDCLKNKVHTLVEKP
                     MALSVSEMDGMVSEAKARSVKLGVCFQNRFNPSVQEVVKKLRENAFGRVFTATARILW
                     NRDEAYYKQANWRGTWRFDGGTLMNQCSHNIDLLQWILGGEVDQVSAVIRNYRHPYLE
                     VEDFGAALISFKNGSVGILEGTANVYPRNLEETLSIFGETGTVVLGGLAVNKIQTWQF
                     PGESGHPFNALPDPESVYGSGHIPLYQDFYEAIAQNREPAISGEEGKKAVEIILGIYR
                     SHKEKRAIPFPLAPFQTTDMAGVKL"
     gene            complement(5775..7079)
                     /locus_tag="PKM49_05355"
     CDS             complement(5775..7079)
                     /locus_tag="PKM49_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNY82274.1"
                     /translation="MLIEKIERRTAVIGVIGLGYVGLPLAVEKAKAGFPVIGFDIQEK
                     RVAMVNEGHNYIGDVVDEDLERLAKSKKLRATTDFDLLRECDVMAICVPTPLDAFKQP
                     DLSYVIRSAKEISKRLRRDTLVVLESTSYPGTTEDVIKPILEETGMVCGRDFFLAFSP
                     ERVDPGNLRFKTKNTPKIVGGYGAMSTKTAKSLYEKVLDAAVFTVSSPKEAEMAKVLE
                     NTFRNVNIALINEMTILAQKMGIDIWEVIEAAATKPFGFMPFYPGPGIGGHCIPIDPF
                     YLTYIARKHDSHTRLIEAASEINDEMPQYILRRLMMLLNESKKCLNGAKIILLGIAYK
                     GDIDDTRESPALKVLELLYQAKADVTIVDPYVEAFKWEGIVKRTEPLLRNRVREADAV
                     LITTAHKKGVDYRMVCEEASLVFDTKNILKTFDVDRSHVFVL"
     gene            complement(7073..7852)
                     /locus_tag="PKM49_05360"
     CDS             complement(7073..7852)
                     /locus_tag="PKM49_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNY82275.1"
                     /translation="MSISKDATIHPSAIIGEGAVIEEGVVIGQNCRLGHHVIVHRGTR
                     IGDNVIISDQTVLGKSPFKAVLSAITADKTFDPLQIKEGCVIGALCIIYLGATLAEQV
                     FVGDLASIREEVSIGRRTIIGKGVTVENRTRVGARCKIETNAYITALSRIEDDCFIAP
                     EVTFTNDNYLGRTEERKKHFKGVTLLRGARVGANATVLPGLTIGEDGLLAAGSVLTKD
                     LPAGKVFLGVPARYFGDVPEEQLLKNQTFYIAETQKGDEGC"
     gene            complement(7858..9033)
                     /locus_tag="PKM49_05365"
     CDS             complement(7858..9033)
                     /locus_tag="PKM49_05365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNY82276.1"
                     /translation="MRVPLFDLTRQYAQLRTEILSTLDRVFSSGQLILSEEVVAFERE
                     IAVLLGAKEAIGVANGSDALYIALKALNIGEGDYVITTPYTFFATVSAIVRNGARPLF
                     ADIDPITMNVDLDQVERLLQTHPERHRVKAFIPVHLFGQTCDLDRLASIKSTFGIRVV
                     EDIAQSIGSTWTDKTGGLRYSGTVGDLGTLSFFPTKNLGAFGDAGMIVSSDPELAEFC
                     RSFRVHGSRERYYHDVVGVNSRLDEVQAAILRIKTRRLGEWTEKRIAIAALYNRLLSS
                     KEAPSERLVLPQVYTDRRHIFHQYVVLLKGNERDALKAFLEQNGIGTSIYYPLPLHLQ
                     KCFADLGGRPGDLPVSERTALRSLALPMFPELTEEEIEYVVERVSAFFAQHPKSANR"
     gene            complement(9049..9717)
                     /locus_tag="PKM49_05370"
     CDS             complement(9049..9717)
                     /locus_tag="PKM49_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HNY82277.1"
                     /translation="MDIDAYSFLNPSPNLREMTVLRLLTQNPSLSQEALARSVGVVPS
                     MVNRYLKEFEDEGLLIKEGENRRNMRYVLSENGKYRLQYLTIAFLSEVAKLYSESRGI
                     FRDVFDYLEKTHYRRLLLYGAGIIGGILVDALKTEGFELVGFIDDSSIKQHDTFRGLR
                     VYAPEEVLQMEYDGVIIASFRHAKRITAKASSLQLKSILIFKIADDGKVSLAISDQLS
                     QPEA"
     gene            complement(10065..>10289)
                     /locus_tag="PKM49_05375"
     CDS             complement(10065..>10289)
                     /locus_tag="PKM49_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82278.1"
                     /translation="QTSLFDRCFLTFPIGVDTRSGEEGLSSCLKCPCNRAVACTPPEE
                     SPVSVSLQDFLLSSLHSKQLDFRIHAFRGY"
BASE COUNT         2423 a         2659 c         2783 g         2424 t
ORIGIN      
        1 ccgtgatctc cttctgcgct ttctggctga aagaggcgag tagaaggcga taggactcgt
       61 cgatcatatc tttcagaacc gcgtctggaa cgttgcctcg aaggttgatc gagttccagt
      121 ggactttatt catataatac ccgggagcga tatcggcgta ggtttctcgc aacgctattc
      181 cgcgcatagg ttcgagtttt agtgtgatga tgggttgttt ctctttgttt tcgcccacca
      241 tcgcgtacat tttcccacgc agcatatacc tcgtggcctc ccattcctct ttgaagtctt
      301 tggtcgttcc cagtttggaa aggcaatatt catcgagcca agcgtacttc atcgtaacct
      361 ccctgtgttg cccttatttt catatcttcg ggtcagcgat gtctcgcaac ccgtctccca
      421 acaaagtaaa ggcgaggacg gcccacgcga tcataatccc cgggaagacc gagagccaag
      481 gcgcccgggt caggaaccgc tgggattcca ttaaaagacg cccccaactg gggaagggcg
      541 gttgcgatcc gaggcccaaa taactcaatc ccgattcgga taagatcgta taggcgaagc
      601 tcgaactggt caaaacggtg agcggggcca ggatgctcgg caagtaatag aacaaaatct
      661 gccgtgggta ggaaacgccc atcgctcgcg cagccactgc gaagtttttt attcttactt
      721 gtctgacgga gtttctgacc tctctggcaa agatcgggat tctggcgatt ccgatggcta
      781 tggtcgcgtt cttcacgttc gaacccaaaa cagagatgag ggcgatggcg aaaagaatgg
      841 cgggaaaagc gtacagtcca tccatgaggc gcataatgat ctcatccacc cacttggaag
      901 agaggcctgc gagaagtccg agcagcaacc cgaagcccaa tgaaatcgag gtggagacca
      961 accccaccaa aagggcagtt tgactgccga ccatacaccg gctgaaggtg tccctcccga
     1021 aatcatcggt tccgaaaggg tggttccaag aaggtggttg gaaacttgcc ttcggattca
     1081 tcttgtaggg gtcgtgaggt gtaaagagaa ggctcgtcgc cgtcaagagg cacaaggaga
     1141 aaaggagaag gaagcccacg atgaaaccgt gatggcgacg gatttttctg aacgtgcggc
     1201 gaagctcctc cgaactatcg aacgcttccg ttcttttctt ttcgcttttt ttcataacga
     1261 cactcttggg tccagtagca tatgtacgaa atccgtcacg atgttgatga ggacggtcat
     1321 tgtggctatc cagagcacga ccccttgcac cacgggaagg tcacgacgtt ggaccgccaa
     1381 cagcaagaga cgcccgatgc ctggaataga gaagacgttt tcgataacga tcgtccctgc
     1441 gaggatcgag gagatttgaa ggccgaatag ggtgacgatc gggcccagcg cattcttcag
     1501 gatatgcttg tagacgatct ttctctctgg gactcctttg cttttcgccg tacggacgaa
     1561 gtcttcgcgc gcattttcca gaagggaaga gcgcaccata cgcatcatta tcgagatcgc
     1621 aacgagagag accgatagag aaggtagaaa aatcgttcgc aaccaagaga aaaaaccggt
     1681 tcgaaggggc gcatactgtc caaaagggaa ccattcgagc ttcaaggtga agacaaagag
     1741 caacaggatc cccatccaaa acgaggggat aacgatgccg agttgagaac ccagagtgag
     1801 cgcgtaatcg aaccaccgat tgtggcgctt aaaggccaag atgccgagga acagggagag
     1861 gacgagggtc agcgcgctac cgacgatcgt aatcgacaac gtgggcggga gggattccac
     1921 gatcacttgc gttacgggtt ttcggtaggt tatcgaacgg ccgaaatcac cggaggcgag
     1981 atcagcgatc caatgaccga atcgaaggat agcgggcgct tccagattca actcttttct
     2041 caatgcggac aaaacctcag agtcggcgtc caatccaagc atcacgagag cggggtctcc
     2101 agggagtagg ttgaagatga aaaaagtgag caaagcgacc gcgagtattg tgatgaaccc
     2161 ggtgatcaat cgttttccga aaaggacaag aagcacggcg cacccaatgc gagagcggta
     2221 ggccgctcag ggaacgacga tctcttcgaa cgaaaagttc tgattggtgt agatacaaat
     2281 ctcgctggcg atttgtagag accgctcgac gatttccttc gcgtcgaggt cggtgtttcg
     2341 atataacgct tttgcggagg ccaacgcgaa gcccgctccc gaacctatgg cgacgagctc
     2401 ttcatccggt tggatgacct ctccgtttcc ggagatcacc aggattgttt cgccatctgc
     2461 tacgatcaga aaggcttcca agtgacggag tatccggtct gttcgccact ctttggccag
     2521 ttcgacggcc gctcgctgga gctgctggga atgcttttta tacttttcct cgaacttttc
     2581 aaaaagggtg aacgaatcgg cgacagaacc cgcaaaaccg gctatgactt tgccgtcccc
     2641 gagttttctt accttccggg ctttcgcctt caagacgctc tgtcccaagg tcacttgtcc
     2701 gtcgcttccg ataacggttc ttttgccccg atgaacgact aagacggtcg ttccgatcat
     2761 agcttccata gtttatctcc tttttctgga ctcttcacaa tagattttat caaatttcgt
     2821 gcccaatcgg caaattcggt gtgggtataa tagcaaggcg cagtaaaaaa gtaaagagat
     2881 ggaggggaaa attactcttg tgggaagcgc agtagagctc atttgcccct tcaaggggag
     2941 taaggtagtg tttttttcga ccccgcgata cgtactttgt ttttttaagc ttagcgatga
     3001 gccagtaaaa aagcgtatcg cgccgtcgtg aaaagaaggg gcgcttacgc cgcgatgccc
     3061 gcgatgtccc gatgcctgtg tgggggtatc gcgcggtcgc aaaaagaaaa ggcgcttacg
     3121 ccgcgatacc cgtgtaggtt atcgcggggt aaccaacggg accccgcgat acgcctttcg
     3181 tctttctcaa atgcgtatcg cgccgtcacg aaaagaaggg gcgtttacgc cgcgatgccc
     3241 gcgatgtcac gatgcctgtg tgggggtatc gcacggtcgc aaaaagaaaa ggcgcttacg
     3301 ccgcgatgcc cgtgctgtcc gtgtgggggt atcgcgccgt cgcgaaaaga aaaggcgctt
     3361 acgccgcgat gcgcccttta cctttctaca acgcgataac tatctagaca aaaaatcgtg
     3421 cgttcgattc tggccctgct tatcgattac tcttcataac tcgactccac gcgaatttat
     3481 gagggtaccg agcggatgag cgggaggaag cgaatcgtct tccgttcctt gagaaggtga
     3541 gcgatggaag gcggctgaaa ataatgatag atattaaaaa agggcggtat cccgccctta
     3601 ttctatatgg ctttctgaag cacctgcact cctattaccg gtttctttcg ggctttcttg
     3661 actttcagtt tggcctcttt tctgttgttc gcttcgatgg tgtacgattt aatccggcct
     3721 tcttcgttga cctctacttt aaatagcatc tccatcagcc tccctcggtt tcataatgaa
     3781 attcataaga aaaagtgtgc tataatttct tggctctcac cattattatt gacggatttt
     3841 cggtagatgt ttttttgaga tgcgagttcg gttttcaaag agcgcctagt gtccctctat
     3901 tataccaaaa aggcgctcga aataaaaacg cgtttggttt tttcactgag ggtcggatag
     3961 gcagaagcac cctgtcaaag tagggtcttt ggaatcggaa atgccaccag aagtactgtg
     4021 cggaataacg ttttcataga gaataactcg aaggcgcttt ttcgtctaag agaggataaa
     4081 tgctagagaa aaaaggagga atgacgatgc tcaacccgaa aatggaaatg gaactgaata
     4141 aacagatcaa cgaggaactc tactcggcgt acctctatca atcgatggcg gcctacttcg
     4201 cggcaaacaa tctcaatgga ttcgccaaat ggatggacgt ccaggctgcc gaagaggtga
     4261 accatgcgag gaagttttac catttcatca tcgatagaat gggaagggtc gttctcgaag
     4321 cgatcgagaa accacctctc gagtgggata gcccccttgc cgcgatggaa gcagcgctca
     4381 agcatgaaca gcacgtcacc ggtcgaatcc actttttagt cgaccttgcc agagaggtta
     4441 aggacaaagc gacggaggtt atgctcaact ggtttgtgat ggaacaagtg gaagaagaag
     4501 cctccgtgga cgagtacgtt caaatgctga agatgttcgg cggaaaaccc caaggcttga
     4561 tgatgatcaa ccgcgagatg gggaaaagag aagacgatta ggcaagaaac gcttttttaa
     4621 agaaaaaacg cccgacgacc gatcgggcgt tttttatttg tggaatagag cgctagaaat
     4681 gttacagctt gaccccagcc atatcggtgg tttggaatgg ggccaaaggg aacgggatcg
     4741 cccgcttttc cttatgtgag cggtaaatgc ctaaaatgat ctcgacggct ttttttcctt
     4801 cttcaccgct gatagctggt tcacggtttt gcgcgatcgc ttcgtagaag tcttgatata
     4861 gcgggatatg cccggatccg tacacacttt ccggatccgg taacgcgttg aacgggtggc
     4921 cggactcgcc ggggaattgc caagtctgta tcttgttgac ggccagtccc cctaaaacga
     4981 ctgtcccggt ctctccaaag atcgaaaggg tctcttcaag gttccgcggg taaacgttgg
     5041 cggtcccctc gaggatgccg acgctcccgt ttttgaagga gatcagcgcg gcgccgaagt
     5101 cttcgacctc caagtagggg tgacggtagt tccgaatgac cgcgctgact tggtctactt
     5161 cgcccccgag gatccactgt agcaaatcga tgttgtgaga gcattggttc atcaacgtgc
     5221 ccccgtcaaa acgccacgta ccgcgccagt ttgcctgttt gtaataggcc tcatcgcgat
     5281 tccacaggat acgcgcggta gcggtgaaga cacgcccgaa ggcgttttcg cgaagcttct
     5341 tcaccacctc ttgaacggaa gggttaaagc ggttttgaaa gcatactcct aatttgaccg
     5401 agcgcgcttt cgcttccgaa accatcccgt ccatctccga cacgctgagg gccatcggct
     5461 tttcaaccaa cgtgtggact ttgtttttca agcagtccat agcgatggtg tagtgatttc
     5521 cgctctccgt cgcgatggcc gccatctcga ttccggtact tagcgtcttt cggtagtcgg
     5581 tgaagacgac cggtcgtttg ccggcgcgcg tcgcgtacgc atccgcaact cgctcggcgc
     5641 gttcgggctt caggtcgcat acggcgatta ggtcgagccg ctcacggttc gctagaaagg
     5701 cttcgaggtg cttgtttgac ccgatgcgtc cgcatccgat caatgccgct tgaattctca
     5761 tcgcagcgcc ccctttagag aacgaaaacg tgcgagcggt ccacgtcgaa cgttttgagt
     5821 atgtttttcg tgtcgaagac tagggatgct tcttcacaga ccatacgata atcgactcct
     5881 tttttatgtg cagtggtgat taacacggca tccgcttctc gcacccgatt tcgaaggagc
     5941 ggctcagttc gtttgactat accttcccat ttgaaggcct ccacgtacgg atccacgatc
     6001 gtcacatccg cttttgcttg gtacagcaat tccagaactt tcagcgcggg tgattcccgc
     6061 gtatcgtcta tgtctccttt gtaagcgatg ccgagtagga tgatcttcgc cccgttcagg
     6121 catttcttgg attcgttcag caacatcatc agccttctga ggatgtattg gggcatctcg
     6181 tcgttgattt cgctcgcggc ctcgatcaat cgcgtgtgag agtcgtgctt cctcgcaatg
     6241 taggtcagat agaaaggatc gatagggatg caatgcccgc cgatgcctgg gcccgggtag
     6301 aagggcataa atccgaaggg tttcgtcgcc gcggcctcga tgacctccca aatgtcgatt
     6361 cccatttttt gcgccaggat cgtcatttcg ttgatgagcg cgatattgac gtttcggaag
     6421 gtgttttcca gaaccttcgc catctcggct tccttcggac tggatacggt gaagaccgcg
     6481 gcgtcgagca ccttctcgta caaggatttg gccgttttcg tagacattgc gccataccca
     6541 cccacgatct ttggcgtgtt tttcgtcttg aagcgcaaat taccgggatc cacgcgctcc
     6601 gggctgaaag cgagaaaaaa gtctcttccg caaaccatac ccgtctcttc cagaattggc
     6661 ttgatcacgt cctcggtcgt tcccgggtag gatgtggact ccaaaacgac cagtgtatcg
     6721 cgacgtaacc gtttcgagat ctctttcgcc gaacgaatca cataagacag gtccggttgc
     6781 ttgaaggcgt ccaacggagt gggcacacag atcgccatca catcgcactc tcgcaggaga
     6841 tcgaagtcgg tcgtcgcccg cagcttcttg cttttcgcta ggcgctccaa atcttcgtcc
     6901 accacgtcac cgatgtaatt gtgaccttcg ttgaccatcg ccacgcgctt ctcttggata
     6961 tcaaagccga tcaccgggaa tcccgctttc gccttttcca cggccagcgg caggcccacg
     7021 taccctaacc cgatcacgcc gataacggcg gttcgtcttt cgattttttc gattaacatc
     7081 cttcatcccc cttttgggtt tcggctatat aaaaggtttg gtttttcagt aattgttctt
     7141 cgggcacatc tccgaaatat cgagccggaa ccccgaggaa aaccttaccc gccggcaggt
     7201 ctttggtgag cacgctccca gccgccagca agccgtcctc tccgattgtc aggccaggca
     7261 gcaccgtcgc gttcgcgccg acgcgggcgc ctcgaaggag tgtgacccct ttgaaatgct
     7321 tcttccgctc ttcggtacgt cccagataat tgtcgttcgt gaacgtgacc tccggggcga
     7381 taaagcaatc gtcttctata cgggagagcg cggtgatgta ggcgttggtt tcaatcttgc
     7441 atcgcgcgcc tacccgtgtg cggttttcga cggtgacccc tttaccaatg atcgtccgcc
     7501 ggccaatgga cacttcttcg cggatcgaag ccaagtcgcc gacgaagacc tgttcggcca
     7561 aagtggcgcc gaggtagatg atgcagagtg cgccaatcac gcacccctcc ttaatctgta
     7621 gggggtcgaa ggttttatct gctgtgatgg cgctcaagac cgctttgaaa ggggatttcc
     7681 cgagaactgt ctggtcgctg atgatcacgt tgtctccgat tcgggttccc cgatgaacga
     7741 ttacatgatg gccaaggcga cagttttgtc ctatcacgac tccctcttcg atcaccgcgc
     7801 cctcgccgat gattgcggaa gggtggatcg ttgcgtcttt agaaatcgac atccgtatca
     7861 ccgattcgcg cttttgggat gttgggcaaa aaaggcgctc acgcgttcga ccacgtattc
     7921 gatctcttcc tcggtcagtt ccgggaacat cgggagggcc aacgaccgta gggccgtccg
     7981 ctccgagacg ggtaagtcgc cgggccgccc tcctaggtcc gcaaaacact tttgtaggtg
     8041 taaaggcaag gggtagtaga tggacgtgcc gataccgttt tgctcgagaa aggccttgag
     8101 cgcgtctctt tcgttgccct tcagaaggac gacgtattgg tgaaagatat gccggcgatc
     8161 ggtataaacc tgagggagaa cgagacgttc cgagggcgcc tctttcgatg agaggagccg
     8221 attgtagagg gcggcgatcg caatgcgctt ttcggtccat tcgccgagcc ggcgggtttt
     8281 gatccgcaag atggccgcct gaacttcgtc caaacgggag ttcaccccaa cgacatcgtg
     8341 atagtagcgc tccctggagc catgaacccg gaaagacctg cagaactctg ccaattccgg
     8401 gtcagaggat acgatcattc ccgcgtctcc gaaggcgccc aggtttttcg ttgggaagaa
     8461 agacagggta ccgaggtctc ctaccgttcc agaatagcgg agccctccag ttttgtctgt
     8521 ccaggtcgat ccgatcgatt gcgcgatatc ctcgaccaca cgaatgccga aagtggattt
     8581 gatcgatgcc aaccggtcga ggtcgcaggt ttgaccgaag aggtgcaccg gtatgaaggc
     8641 tttcacgcga tgccgttcgg gatgcgtctg gagcagtcgc tccacctggt cgaggtcgac
     8701 gttcatcgtg atcgggtcga tatcggcgaa aagcgggcgc gcaccgtttc taacgatcgc
     8761 cgaaaccgtg gcgaaaaagg tgtagggcgt ggtgatcacg tagtctcctt caccgatgtt
     8821 gagggctttc agcgcgatgt acaacgcgtc ggaaccgttc gcgacaccga tggcttcttt
     8881 ggcccctaat aggacagcga tctctctttc aaaggcgacc acctcttccg ataatatgag
     8941 ttgtccagaa gagaagaccc gatcgagtgt cgacaggatc tcggtcctca actgcgcata
     9001 ctgccgggtt aagtcgaaca aaggaacacg catagttcac ctctgttttt atgcctccgg
     9061 ttgggaaagc tggtcgctga tagccagcga aaccttcccg tcgtcggcta ttttaaagat
     9121 caaaatagac ttcaattgca gggaagaggc cttggcggtg attcttttag cgtggcgaaa
     9181 agaggcgatg atgacgccat cgtattccat ctggagaacc tcttccggcg cgtagacacg
     9241 caatccgcgg aaggtgtcgt gttgtttgat cgaagaatcg tcgataaaac cgacgagctc
     9301 gaacccttcg gttttgaggg cgtcgacgag aatcccgccg ataatccccg ccccatacag
     9361 cagcaaccgc cgataatggg tcttctccaa atagtcgaag acatccctga atatcccccg
     9421 agattcagaa tacaacttgg cgacttcgga caaaaaggcg atcgtcaggt attggagccg
     9481 gtatttcccg ttttcggaca agacataccg catattccgc cggttctctc cctctttgat
     9541 caacaggccc tcatcctcga actccttgag gtaccgattg accatgctgg ggacaacgcc
     9601 aacgcttcga gccaaggctt cttgggagag ggaaggattt tgcgtcagca ggcgaaggac
     9661 ggtcatctcc ctcaggttag gcgaaggatt caaaaacgaa taggcatcga tatccacgaa
     9721 agtcccccct caattattca cacagtgaat gaattatatc atatatcgaa aatcaaaatc
     9781 aaggcgtgcg ggaaaattaa tcgggtgaca taactattag gttcatcgcg gcgtaagcgc
     9841 ttcttcttta tgcgacggcg cgatacgctt gggaacgcca acttttagta cgtccaggta
     9901 aggcggaaat gttgagcagg aaggaaagca agcctgccgg ggaaaaagtc agaaccccgt
     9961 agcttggaca gcaggcaggg aggaaacgaa ctgtctggag tctgttgaca aagccactct
    10021 aagggtggtg agcgagtaac cgggccgtaa caaccgtgaa cgcattagta gcctcgaaag
    10081 gcgtggattc ggaagtcgag ctgtttggag tgcagcgaag acagcaggaa gtcctgaaga
    10141 ctgactgaaa cgggactttc ttccggcggg gtgcaagcga cagcacggtt acaaggacat
    10201 ttcaggcaac tggagaggcc ctcctcgccc gaacgggtgt caacaccgat cggaaatgtg
    10261 agaaaacacc gatcaaaaag tgatgtttg
//
LOCUS       DAOPXN010000070        10076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6738_length_10076_cov_5.443668, whole genome shotgun sequence.
ACCESSION   DAOPXN010000070 DAOPXN010000000
VERSION     DAOPXN010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6738_length_10076_cov_5.443668"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKM49_05380"
     CDS             complement(<1..561)
                     /locus_tag="PKM49_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82279.1"
                     /translation="MYRKKKWLGFLIISPTLAVIGIFVYGFIGATVRASLSDWNSFKK
                     LLSGEFVYTGLRNYERLFADWRFQTDMWNTLFFTVFFLFGCLLIGLILASLLDRPLKG
                     GRVFQSIFLFPMALSLVVTGTVWRWFFAPGILPNDPAGINVLLGQIGLKSLQWNWFTV
                     TDTIGPFNLALIPVIIAAVWQLSGYTM"
     gene            complement(640..1881)
                     /locus_tag="PKM49_05385"
     CDS             complement(640..1881)
                     /locus_tag="PKM49_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82280.1"
                     /translation="MRKFIVLMCAILIAVVAFGSQVEIFSWWTGGGEEAGLLALIKLF
                     NQLYPGVQVINATVAGGAGTNAKAVLKTRMLGGNPPDSFQVHAGMELSDTYVIPGKME
                     PITGYLKEWGIFDKFPEDIMKICTYNGEVYSIPVNVHRGNVVFYNTAIAKEIGMKTPP
                     KTWKEFFELLEKAKKAGYVAISLGDTGKWTATHLFESIMLSQFGPYKYNMLWTGDQSF
                     NHVALTESFEIMKKITPYFNKDHAAGDWQDASRLIFEKKAFCNVMGDWAEGYFKEMGW
                     TPGVEFGWFAVPETDVAFMIVSDTFGLPKGAPNKENALRWLKLVGSVEGQDLFNPLKG
                     SIPARVDADKSKYDVYLQWAMADFGVKALSPSIAHGSAAPESFATMLNDILNRFVSDL
                     NVNQAVKSVLEAAEEEGYIVK"
     gene            complement(2231..2482)
                     /locus_tag="PKM49_05390"
     CDS             complement(2231..2482)
                     /locus_tag="PKM49_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY82281.1"
                     /translation="MRDLEIYEIVKGIIVKRMGVSASTVLPQSSLTEDIGADSLELVD
                     LMMDLEEILGIRIENSELSDVETVRDVVNLIESKRRVSV"
     gene            complement(2460..3458)
                     /locus_tag="PKM49_05395"
     CDS             complement(2460..3458)
                     /locus_tag="PKM49_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNY82282.1"
                     /translation="MSLFNTNRICGLLGVKYPFIEGGMAWVGTPRLAAAVSEAGGLGT
                     IGSGALTPEDLAESIDTTRRQTNKPFAVNVMLLNPSADRQVEVCLQKAIPVLIFGAGN
                     PAKYLPDLKKRGIVTAAVVASETLALYLEERGIDVIIGEGMECGGHVGTVTTFTLIPA
                     LKDVLFSVPVVAAGGIADERGIKAAFCLGAEGVQIGTRLIATVECEAHDRYKQRIITS
                     GIRDTLLTGQALGHPARVLKSAFAKRVCKLEASDPKEAESLITGSLRKAFEDGDEERG
                     LFMAGQSAGLIQSIPSVSELFELWAQRLDGWNQIAGTETKIAENLKEGVYERSGNL"
     gene            complement(3494..4756)
                     /locus_tag="PKM49_05400"
     CDS             complement(3494..4756)
                     /locus_tag="PKM49_05400"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNY82283.1"
                     /translation="MNKVAVTGIGTINAIARRTEEFISALRAMRCGIEKITQFDCSDY
                     PAAMAAEIKDFSPEQHFELKIANRYDRFLQLGIVAARNAISDAGLEPSGSWRENAAVT
                     VSSGIGGFKTLHREYGNLFKRGPKAVRPHLIPMMISDMASGAIALDLGCHGPNFGVVS
                     ACASALHAIIITALLIRNGLTEVALTGGTEAVIDPMPVAAFGSMTALSPERIDPLKAS
                     RPFHSERNGFVMGEGAGVLVLESEAHASKRGARIYGWIEGFGMSEDAYHISQTDPEGR
                     GAQKSMREALEQAGIGPDQVGLINCHATGTPVGDVSEITALKQVFGDALPHIPIQSTK
                     GLVGHTLGAAGAIESIAAILQAQHGFVHGMPYLDSLDPCCEGLLIHRETTPRSFGVVL
                     KNAFGFGGHNASLVYRKYTKTNEPPAAL"
     gene            complement(4813..5451)
                     /locus_tag="PKM49_05405"
     CDS             complement(4813..5451)
                     /locus_tag="PKM49_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNY82284.1"
                     /translation="MKKDEKYNRIVTAAEKVFFNYGYGQSSMDQVAQEAGVAKGTIYL
                     YFQSKQELYYAIGVRALGILLKSFRELFSSCKSGLEKVVAFGRAFAQYRKSYPDYYQF
                     IVNYQGEPYDERNEYGEVHRTYQESRELFRVLVKAVTEGQADGSIGVKTDPALLAAIL
                     WCQTCGVVQLTALREPFFKLFTPSSADEMLESYFTLTEKVLEREIRIERVPN"
     gene            complement(5896..6402)
                     /locus_tag="PKM49_05410"
     CDS             complement(5896..6402)
                     /locus_tag="PKM49_05410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HNY82285.1"
                     /translation="MQVRKITEADAAPYLELRCALDSESKTLLLEPGERKTTVDEMRR
                     TIQDILHQPNSMVFLATDEERLVGFLSAIGGKANRHRGTITIVIGILEAYTGRGIGKK
                     LFVEMEKWARGIGAHRLELGLMAHNERALRLYTSLGYQPEGRKRDVFLVDGVYVDELM
                     MGKILETE"
     gene            7074..8675
                     /locus_tag="PKM49_05415"
     CDS             7074..8675
                     /locus_tag="PKM49_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNY82286.1"
                     /translation="MLHVKGITKSYSTRVVLNGLSFDLEESQLLAVTGGNGSGKSTLL
                     KGIVGEISFDEGSVTLDKDVTWAYLPQQLPETDLNAIQYLLSVFPPLTEAFHKITSES
                     DPFAYAEALDAFHSLGGYEVEREIDRNLAYFGFSESDGKKAMTAFSEGEKRLLGIIRL
                     MLSGARLFLLDEPTNHLDIGHCVMLEEYILQKKEERKAFLLVSHDRAFIDRVADRTLY
                     LERGAYFSVEGGYSALLSHKEQSFSTQVRHAQTLQRKIRQLERAITQTKQWAQKTERK
                     KQDNPHADKGYIGHKSARLAKRATGVTGRLSALAQSLKEEKPFVEKPINLSFLEYPVP
                     SRVVLRAQQLEKRFEERTVLSNVSIDCSTRDRIGIIGENGSGKTTLIRILVNDLPADA
                     GDCYRNDAVRWVYLPQDIRQFFAYEVLMDNLTRYDHSEVFIRQALGAARIRNERVFSK
                     VGELSRGELMRCALVAAILAKAEFLFLDEPTNHLDIESLEVLHSLIEQFSGGVLFISH
                     DRYFIAQQAKTLYWLTGGSLRLMEA"
     gene            complement(8707..9120)
                     /locus_tag="PKM49_05420"
     CDS             complement(8707..9120)
                     /locus_tag="PKM49_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNY82287.1"
                     /translation="MSDSGSRSRGRPSQSVPSGEDFSQGSRSGARYEEVCAEYLRKQG
                     YSILERNFSTRLGEIDIIAMDGEDIVFFEVKGGKEDPPPFTRVTPAKLRRILLTVQHY
                     LHHHPEQRYCHVRVDVLSITLPDLKIRHFKDVTAH"
     gene            complement(9117..9401)
                     /gene="gatC"
                     /locus_tag="PKM49_05425"
     CDS             complement(9117..9401)
                     /gene="gatC"
                     /locus_tag="PKM49_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNY82288.1"
                     /translation="MKIDDLLFHRLEKAARLKVSPEERSRLQADIERILDYVELLNEV
                     DVSDLEPMVRPVDLVNQTREDVVAARPFPRKILENAYEEQDGYFVVPKIL"
     gene            complement(9388..9636)
                     /locus_tag="PKM49_05430"
     CDS             complement(9388..9636)
                     /locus_tag="PKM49_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HNY82289.1"
                     /translation="MLVLSRKKDEEIYIETTIGNIILKIVDIDEKNVKVGITAPKNMP
                     IYRREVYEKVYAVNKESVLHDDLKEITKVLGALKREDR"
     gene            complement(9624..>10076)
                     /gene="fliW"
                     /locus_tag="PKM49_05435"
     CDS             complement(9624..>10076)
                     /gene="fliW"
                     /locus_tag="PKM49_05435"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNY82290.1"
                     /translation="LGKIEIEESEVLTFETGIPGFEAWHKYALVMLPETLPVQWLISV
                     ESMEIAFPVLDPWLVKKDYAFDISQDVIDRLDIKDRDKILVTNMVRIPQDNPQEMTIN
                     LLAPIIINTENRKAIQLVLDRSDYSLRHLIREGASEPASKKEGEVCSS"
BASE COUNT         2323 a         2755 c         2581 g         2417 t
ORIGIN      
        1 catcgtgtac ccggacaatt gccaaaccgc ggcgatgatc accggaatca gcgcgaggtt
       61 gaatggacct attgtgtcgg tcaccgtgaa ccagttccat tgtaagcttt taagcccgat
      121 ttgccccaac aacacgttta taccagcagg gtcgtttggc aggatgcccg gcgcgaaaaa
      181 ccatcgccac accgttccgg taacgacgag cgacaacgcc atcgggaaaa ggaagatgct
      241 ttggaaaacg cgcccaccct ttaaaggcct atccagaagg ctcgctaata tcaatccaat
      301 caggaggcaa ccgaacaaga aaaacactgt aaaaaacagg gtgttccaca tatctgtctg
      361 gaagcgccag tctgcgaaaa gacgttcgta attgcgaagg ccggtataga cgaactcccc
      421 ggaaaggagc tttttaaagc tgttccaatc cgaaagtgag gcgcgaactg ttgcgccgat
      481 gaagccgtaa acaaagatac cgatcacggc caaggtggga gagataatta agaaccccaa
      541 ccatttcttt ttcctgtaca ttcgtccccc ttcctaccac taggcgaccg agaagagaaa
      601 agagaggtct ctttcgagac ccctcatagg agcacttgtt tacttaacga tatatccttc
      661 ttcctctgcg gcctccaaaa cagatttgac cgcttgattc acgttgaggt cggagacgaa
      721 acggttcaga atatcgttca acattgtggc gaaagactcg ggggccgcgg atccgtgcgc
      781 aatggatggg gagagggcct tcacgccgaa atcagccata gcccactgta aatagacatc
      841 gtacttcgat ttgtctgcgt ccactcgagc gggtattgag cccttcaggg ggttgaacag
      901 gtcctggcct tccacactcc cgaccagttt tagccacctt aaggcgttct ctttgttcgg
      961 tgcgcctttc ggtagaccga aagtgtcgga cacgatcata aaggcgacgt cggtttccgg
     1021 aactgcaaac cacccgaatt ctacgcccgg tgtccagccc atctccttga agtatccctc
     1081 tgcccagtca cccatcacgt tacaaaaggc cttcttctcg aaaataagac gcgaggcgtc
     1141 ttgccaatcg ccagcggcgt ggtctttgtt gaagtagggt gtgatctttt tcataatttc
     1201 gaaagactcg gttaaggcca cgtgattgaa gctttggtcg ccggtccaga gcatgttgta
     1261 tttatacggc ccgaattggc tgagcataat gctctcaaaa agatgggtgg ccgtccattt
     1321 tccggtatcc ccgagcgaga tcgcgacata tccggctttt ttggcctttt ccaatagttc
     1381 aaagaattct ttccaggttt tcgggggagt cttcatgccg atctctttgg cgatcgcggt
     1441 gttatagaac acgacgtttc cacggtgcac gttcaccgga atcgagtaca cttccccatt
     1501 gtaggtgcag attttcataa tgtcctcggg gaatttgtcg aatatccccc attctttgag
     1561 gtatcccgtg atcggttcca ttttccccgg aatcacgtaa gtatcggaaa gctccattcc
     1621 ggcgtgaacc tggaaggaat cggggggatt cccaccgagc atacgcgtct tcaagacggc
     1681 cttcgcattg gtccctgctc cgcccgctac cgttgcgttg atgacttgta ctcccgggta
     1741 aagctggtta aagagtttga tcagcgccaa aagtccagcc tcttctccgc ctccagtcca
     1801 ccaactgaag atttccactt gacttccgaa cgcgacaact gcaatcaaaa tcgcgcacat
     1861 cagaacgata aattttctca tgttcttacc tccccgttag gataatccct gaaatcgggt
     1921 ttttcgccga aagatgaagg ttatgaggtc tttagaaaag attatatcat aaaatagaaa
     1981 ggttgcacac ggtgaaagag cgcgaaataa gcccaaatca gccgaaagag agaggaaaga
     2041 tgaaggattc ttgcccagaa ggaagctgga tggaggttag ccgacttggt tagccgcctt
     2101 taggtttcca gatgtgtttt cgatcgggag cggtggcttg ggatattgtg gatagggcgt
     2161 atggatagcg ctgagggcgg agcgggtccg cggtaccgat tgcggacccc gttgccccct
     2221 cgacgacggg ctaaacgctt actcttctct tgctttcgat gaggttcacg acatcgcgaa
     2281 ccgtttccac atcggataat tccgaattct cgattctaat ccccaggatc tcttcgagat
     2341 ccatcatcaa atcgaccaat tccaaagaat cggctccgat atcttccgtg agcgaggatt
     2401 gcgggagtac ggtcgaggcg ctcaccccca ttcttttaac gataattcct ttcacaatct
     2461 catagatttc cagatctctc atagacgccc tccttaaggt tttctgcgat tttcgtttcc
     2521 gtcccggcta tttggttcca tccgtccagt ctttgggccc atagttcgaa caattcagac
     2581 acggagggaa tcgactggat gagtccggcg gattgtcccg ccataaacag tcccctttct
     2641 tcgtctccgt cttcaaaagc tttcctcaaa ctcccggtga tgagcgattc cgcttctttc
     2701 gggtcagacg cttccaactt acaaactcgt ttggcaaagg cgcttttcag tactcgcgcc
     2761 gggtggccta gcgcctgtcc cgtgaggagt gtatctctaa tgccgctggt gatgattctc
     2821 tgtttgtaac ggtcgtgcgc ctcgcattcc accgtcgcga tgaggcgcgt gccgatctgc
     2881 acaccttctg cgccgagaca gaaggcggct ttgatacccc gctcgtcggc gatcccgccg
     2941 gctgcgacga cgggcaccga aaaaaggaca tctttcaaag cggggatcag cgtaaaggtc
     3001 gtcaccgttc ccacgtgccc gccgcactcc atcccctctc cgatgatcac gtcgatcccc
     3061 ctctcctcca ggtacaaggc cagggtttcg gacgcaacga ccgccgcggt cacgatccct
     3121 cgctttttca aatcagggag gtacttcgca gggttacccg cgccgaagat gagaacgggg
     3181 atggctttct gaaggcagac ctccacctgt cggtctgcgg aaggattcaa cagcatcaca
     3241 ttgactgcga acggcttgtt tgtctgtcgg cgtgtcgtat cgatactctc cgccaaatcc
     3301 tcaggcgtga gggcgccgga tccgatcgtc cccaaaccgc ctgcttctga gacggcggcc
     3361 gccaacctcg gcgtgcctac ccaagccatg ccaccttcga taaacgggta cttcacaccc
     3421 aacaatccac agatacgatt cgtattgaac aggctcattt ttaactcccg atcacaaaga
     3481 ggttccgcgt ttttcagagc gcggccggag gttcgtttgt tttcgtatat ttccgataga
     3541 cgagcgatgc gttgtgtccc ccgaacccga aggcgttttt caaaacgacg ccaaacgaac
     3601 gtggtgtcgt ttctctatgg attagcaagc cctcgcaaca cggatcgagg ctgtctagat
     3661 acggcatccc gtgaacgaac ccgtgctgcg cttggaggat cgcagcgatc gattcgatgg
     3721 cgcccgccgc tcccagggta tggcccacca accctttcgt cgattggatc ggaatgtggg
     3781 gcaaagcgtc tccaaagacc tgttttaggg cggtgatctc cgaaacgtcg ccaaccggag
     3841 taccggtggc gtgacagttg atcaatccga cctgatcagg cccgatgccg gcttgttcca
     3901 aagcttcgcg catcgatttt tgggccccgc gcccctccgg atccgtctga ctgatgtgat
     3961 aagcatcttc actcatcccg aaaccttcta tccacccgta tatcctcgcc cctctcttgc
     4021 tggcgtgcgc ttcgctttct aaaaccaaaa cccccgcgcc ttcccccatc acgaaaccgt
     4081 tccgttcgct atgaaaaggg cggctggctt tcaaagggtc tatgcgctcc ggagacagcg
     4141 cggtcatact cccgaaggcc gcgacgggca tcgggtcgat gacggcctcg gtccccccgg
     4201 tcaaggcaac ctcggtcagg ccgtttcgga tgaggagcgc cgtaataatg atcgcgtgaa
     4261 gcgcagaggc gcaggcgctg accacgccga agttcgggcc gtggcatccc aaatccaacg
     4321 cgatcgcccc ggaagccata tccgagatca tcatcggtat caggtgaggg cgtacggcct
     4381 tcggccccct cttaaacaga ttcccgtatt ctcgatgcaa ggtcttaaac ccgccgatgc
     4441 ccgaagagac cgtgaccgcg gcgttctccc tccaagaccc agaaggttcc agaccggcat
     4501 cggagatcgc gtttctcgct gcgacgatcc caagctgcaa aaaacgatcg taacgattgg
     4561 cgatcttcag ttcgaaatgt tgctccggcg agaaatcctt aatctctgcc gccatcgcag
     4621 cgggatagtc ggagcaatcg aattgggtga tcttctcgat tccacatctc atagcgcgaa
     4681 gcgcactaat gaattcctcg gtgcggcgcg cgatcgcgtt gatggttcct attcctgtaa
     4741 cagcaacttt gttcatagct caccccgaaa tgactatcgg tcatccatag ctaaaaaaaa
     4801 accgtttctt cgctaattcg gaaccctttc gatccggatt tcgcgttcca agactttttc
     4861 tgtcagtgtg aagtatgatt ccagcatctc gtcggcgctg gagggcgtga acagcttgaa
     4921 aaagggttct ctgagggcgg tcaattggac gaccccgcag gtttgacacc ataagatcgc
     4981 ggccaggagg gcgggatcgg ttttcactcc gatcgacccg tccgcttggc cttcggttac
     5041 cgccttgacg agcactcgaa agagttcacg gctttcttga taagtacgat gaacctctcc
     5101 gtattcgttc ctctcatcat agggttctcc ttggtaattc acgatgaact ggtagtagtc
     5161 cggataagat ttccggtatt gggcgaaggc ccgtccaaag gcaacgacct tctccaaacc
     5221 ggatttacag gaagagaaca gttcccgaaa ggatttcagc agtattccga gcgcgcgaac
     5281 tccgatggcg taatagagtt cttgtttgct ttggaagtat agataaatgg ttcctttcgc
     5341 cacgcctgcc tcttgagcga cctgatccat cgaagattgg ccgtatccat aattaaagaa
     5401 caccttttcc gcggctgtga cgatccgatt gtatttttcg tcctttttca tagccctccg
     5461 tagaaatgac cgtgggtcat tgcgcgaagt atatcataaa actcgcggtc ggtcaacccc
     5521 acgagccgga tagcgtgctt cagcgcggca tgctttttta gaagagaaaa gaggggttca
     5581 cacggagaac acagagggca cgaagaaaaa aaacctggga acgccaatct ttagattggt
     5641 atatttcacg gtttttaaag aaaaaaaccc aacctatgcc gaactgaatt tcggcgttct
     5701 cagaaaagta aacccatgcc gcactaaatt tcggcgttcc ctggaaagta aaacgatgcc
     5761 gaactgaatt tcggcgttcc cagaaaagta aaccgatgcc ggaatgaagt tcggcgttcc
     5821 cagaaaagta aaaccatacc gtactgtagt tcagcgttcc caggcgcacc ggcgcgttac
     5881 cagggtaggg atgggttact ctgtttccag tatctttccc atcatgagct cgtccacgta
     5941 gacgccgtcg acgaggaaga cgtcgcgttt cctcccctcg ggctgataac cgagtgaggt
     6001 gtataggcgc agggcccgct cgttatgcgc catcagcccg agttcgaggc ggtgggcgcc
     6061 gatccctcgc gcccattttt ccatctctac aaacagtttc ttccctattc ctcggcccgt
     6121 ataggcttcc aagatcccga tcacgatggt gatcgtcccg cgatgccgat tcgcttttcc
     6181 cccgatggcg ctgaggaatc cgaccagacg ttcctcatcc gtagccagga agaccataga
     6241 gttcggttgg tgtaggatgt cctgtatcgt gcgacgcatc tcatcgaccg tcgtttttct
     6301 ctctccgggt tccaacagca acgtcttgct ctcgctatcc aaagcgcagc gcaattccag
     6361 atacggcgcc gcgtccgctt ccgtgatctt tcgaacctgc acgcgtaaac ctcctcatag
     6421 ggactatgat cgggattata acaaaaactc ccgataatcc tatataatag tgtgagccaa
     6481 gttatgaggt gcaaactaga aagaaggcag aatcaatcgc acgattttta gtccacgtaa
     6541 tcatcgcggc gtaagcgtcc cctcttttcg cgatggcgcg atacgctttt gtatatgcct
     6601 tgaaaagcgg tgttcgcaca gagggcacag agggcacaga gaaaaggctg aagaaagctc
     6661 gtgggaccga cttccagtca gcctgtctct ctgagaaggc cctgggaacg cctgggaacg
     6721 ccaactttaa agttggcttg tttcaaaggt taaacaggtt tcgcccggtg ggcacggaga
     6781 acacagagga aaaactggca agggacaagg ctctcccaga ataagaaaag acatgccgga
     6841 ctaaagtccg gcgtacccag ggcgctcctg cggcttcgaa acaagagcaa aaggcggatc
     6901 gcggggtcga aaaaaacact taccccgcga taaccccgcc ggaccatcgt agcgtaaaaa
     6961 gagcaaaagg cggacctcat ccttttgact ttttcgctac acctcgatag tatacccaca
     7021 ctatattata gggcaggatt gtctctgcca ggtcttgaag agggggtgaa cggatgcttc
     7081 acgtaaaggg gattacgaag agttattcga cacgggttgt gttgaacggc ttatccttcg
     7141 atttggaaga gtcgcagctt ttggccgtca caggcggcaa cggaagcggg aagtccacgt
     7201 tgttaaaagg gatcgtcggc gagatctcct ttgatgaagg atcggtcacg cttgacaaag
     7261 atgtgacctg ggcgtatctt ccgcaacaac ttcccgagac cgacctgaac gcgatacagt
     7321 acctgttgtc tgtttttccg cccttgaccg aggcgttcca taagatcacc tccgagtctg
     7381 atccctttgc ctacgcagaa gctttggacg cgttccattc cttgggaggt tacgaggtgg
     7441 aaagggagat cgaccggaac ctcgcttatt tcgggttttc tgaatctgac ggaaagaagg
     7501 ctatgacggc cttttccgaa ggtgaaaagc gcctgctggg gatcattcgt ctgatgctct
     7561 ccggggcgag gttgttctta ctggacgaac cgaccaatca tctggacatc ggtcattgcg
     7621 tgatgttgga ggaatacatt ctccagaaaa aagaagaacg gaaggctttc cttctagtca
     7681 gtcacgatcg ggcttttatc gatcgtgtgg cggacaggac cctctaccta gagcgtggcg
     7741 catacttcag cgtcgaagga gggtattctg ccctcctatc ccataaagaa cagtcttttt
     7801 ccacccaagt ccggcacgct caaacactac aacgcaagat tcggcaactg gagcgcgcga
     7861 tcacgcaaac gaagcaatgg gcacagaaaa cagaacgaaa aaagcaggac aatccccacg
     7921 cggacaaagg ctatatcggc cataaatccg cacgtttggc caagcgcgca accggcgtta
     7981 ccggtcgctt gagcgcattg gcacaatcgt tgaaagaaga gaaaccgttt gtcgagaaac
     8041 cgattaacct ctctttccta gagtatccgg tcccttcgcg cgtggtcctc cgcgcccaac
     8101 agttagaaaa gcggtttgag gagcggacgg ttttgtccaa tgtctctatc gattgctcga
     8161 cccgtgaccg gatcggcatc ataggagaaa atgggagcgg caaaaccaca ttgatacgga
     8221 ttcttgtcaa tgatctgcct gcggacgccg gtgactgtta ccgtaacgac gctgtccgct
     8281 gggtatacct cccgcaggat atccggcagt tcttcgccta cgaggtgctg atggacaacc
     8341 tgacgcgtta cgatcactcc gaagtcttca ttcggcaagc gttaggcgcg gctcgtatcc
     8401 gaaatgaacg ggtcttttcg aaggtgggtg agctcagtcg cggagaattg atgcggtgcg
     8461 cgctggtcgc ggcgattttg gcgaaggcag agtttttgtt cttggatgaa ccgaccaatc
     8521 atttggatat cgagtctttg gaagttttac actccctgat cgaacagttt tccggcgggg
     8581 ttctcttcat cagccacgac cggtatttta tcgcgcagca ggcaaaaacc ctctactggt
     8641 tgactggggg gagtctgcga cttatggagg cttaaaagac tacgcctacc gaatcatcgc
     8701 ggcgcgctaa tgggccgtga cgtctttgaa atggcgtatt ttcaagtccg gcaaggtgat
     8761 cgagagcaca tccacccgaa cgtgacaata ccgctgttcg ggatggtggt ggaggtagtg
     8821 ctgtaccgtt aaaaggattc ttctaagttt ggccggcgtc acgcgagtga acgggggcgg
     8881 gtcttcctta ccgcctttga cttcaaaaaa gacgatgtcc tcgccatcca tcgcgatgat
     8941 gtcgatctcg cccaagcggg tgctaaagtt ccgctccagg atgctgtacc cttgttttcg
     9001 caggtactcc gcgcagacct cctcataacg cgcgccagag cgtgaccctt gtgaaaagtc
     9061 ttcacccgag gggacacttt gtgaggggcg cccacgcgac cgtgaacccg agtcgctcat
     9121 aagatcttgg ggaccacgaa atatccgtct tgttcctcgt aagcgttctc aaggatttta
     9181 cgagggaaag ggcgcgccgc gacgacgtct tcgcgggttt ggttcaccaa atcgacagga
     9241 cggaccatcg gttcgagatc cgagacatcc acttcgttca aaagctctac gtagtccaga
     9301 atcctctcga tatccgcttg aagtcggctc ctctcttccg gactcacctt gagcctcgcc
     9361 gccttttcta ggcgatgaaa caagagatca tcgatcttca cgcttgagcg cccccaatac
     9421 tttcgtgatt tcttttagat catcgtgtaa caccgattct ttgttgacgg cgtaaacctt
     9481 ctcgtagact tcccgacgat aaatcggcat attcttgggg gccgtaatgc ccaccttcac
     9541 gtttttttcg tcgatgtcaa cgatcttgag gataatgttc ccgatggtgg tctcgatgta
     9601 tatctcttcg tcttttttcc ggcttaggac gagcatactt cgccctcttt tttcgaagcg
     9661 ggttcgctcg ctccctctcg tatgagatgt cgtaggctat aatcactgcg gtctaaaacc
     9721 agctgtatgg ctttccgatt ttcggtgttg atgatgatcg gggccagcag gttgatcgtc
     9781 atctcttggg gattatcttg gggtatccgt accatgttcg tcaccagtat cttgtctcgg
     9841 tccttgatgt ccaaacggtc gatcacgtcc tgcgagatgt cgaaggcgta gtcttttttc
     9901 accaaccacg ggtcgaggac ggggaaagcg atttccatgc tctccaccga tatcagccat
     9961 tgaaccggca gggtttccgg caacatcacc agcgcgtact tgtgccaagc ctcgaagccc
    10021 ggaatccccg tctcgaaggt gagaacctcg gattcttcta tttctatttt ccctaa
//
LOCUS       DAOPXN010000071        10033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6777_length_10033_cov_5.531369, whole genome shotgun sequence.
ACCESSION   DAOPXN010000071 DAOPXN010000000
VERSION     DAOPXN010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10033
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6777_length_10033_cov_5.531369"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKM49_05440"
     CDS             complement(<1..297)
                     /locus_tag="PKM49_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82291.1"
                     /translation="MNYIFFPFKAIGYLAYYTCIMLYYLLYSLYYTWRIRWLEKRRGE
                     EAAAVYARSKGVEISRRSYLWFGMKPEIDGFENLPKDRGYMIASNHLSAFDASLV"
     gene            complement(325..1707)
                     /locus_tag="PKM49_05445"
     CDS             complement(325..1707)
                     /locus_tag="PKM49_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HNY82292.1"
                     /translation="MSGFQIRGVVEGFYGPPWTMEERTQIIQEMARLEMNLYVYAPKN
                     DLLHRHRWETPYPQEFIREFNALVEAGKGSGVGVALALSPGLTLTYSDFGQIDALVRK
                     YLSFAAIGVSDFCLFLDDIPLSLQKEADRGTFASLADAQVFFTNHVYERLKNLTAVTS
                     FLFCPTQYCGNGQTPYLKEIGSGLHPDIDALWTGPQVCSRTIPFEDAETVSRTLRRKV
                     VYWDNYPVNDGSMACELHIGPYTGRDVRLPTVSRGFLINPMNQAMASIPVLTSIAAYL
                     KDPATYDPLSAWESALEAFAPHCAQSLKLFAEMNLISPLNPKGEEMVSDRWIDAYSSS
                     ARSGSIAQAASLLQQHALTLNEAVQTLKTTMPLAWLREVRPWLVEFESWGALLLKAAE
                     LAGMFPLFFQETLDRETLDKSANALADLKTRVSKTVDHQTVCAGSGIREFAMTMIAKI
                     GALLRLRSWE"
     gene            complement(1898..2326)
                     /locus_tag="PKM49_05450"
     CDS             complement(1898..2326)
                     /locus_tag="PKM49_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82293.1"
                     /translation="MKKLLVISLMVLVLTFATSGCYVALQAYPNITGYWTFTTTIPGL
                     ATQLIHIWSDNFGGLGGMTHGAPFNGFAITGTISGAKFGIPAKMEISFTHTATGNVYH
                     FSAELDDMVLRYQKKMTGTVTRTLGAASTGGTFEAEWEHL"
     gene            complement(2364..2735)
                     /locus_tag="PKM49_05455"
     CDS             complement(2364..2735)
                     /locus_tag="PKM49_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82294.1"
                     /translation="MKKVRIVLLLSLLLLMALFMTNCSVTVGWPFSWLGGPWAVTFDW
                     GFLLGEARAPDPDGIVMNFTTGKFSIDGVDHDFRYNADSQGNINILVGDQVLNGKIDR
                     TSPKIVGTVEKDGNRFEAVKQ"
     gene            complement(2835..3419)
                     /locus_tag="PKM49_05460"
     CDS             complement(2835..3419)
                     /locus_tag="PKM49_05460"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNY82295.1"
                     /translation="MSETKTVVLVGVGGQGILLVSKVLSELLVENGYDVKMSEVHGMA
                     QRGGSVSTQIRFGKKVYSPFVGHGGADILLGFEKMETYRFLPYLKAGGTVIYADTMIP
                     SAPIFSAKKEYPQGLDDEIEKTGRALRVPVTEWAAEIGGTSYQNTVLLGVLAKTLGIG
                     YDQTEAVLMKLIKERFHEKNQTALAKGYGFTQKN"
     gene            complement(3412..5199)
                     /gene="iorA"
                     /locus_tag="PKM49_05465"
     CDS             complement(3412..5199)
                     /gene="iorA"
                     /locus_tag="PKM49_05465"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HNY82296.1"
                     /translation="MGKVLLSGNEACARGAYEAGVQVACGYPGTPSTEILESLARYRE
                     IDAEWSINEKVALEIVFGSAFAGLRSLATMKNVGLNVAADPLFSGAYLGVEGGCVILT
                     ADDPGLHSSQTEQDNRHYAVHAKVPLFEPSDSQEAKDMMKMAFEVSERHRIPVLFRMT
                     TRVCHSKGLVELGERIEKPPTTYRRDLSRFVATPARCRVHHAELELKLKELEEWSNET
                     ELNFIEPGDSKTGVITSGIAYQHAREVFGSRATYLKLGFTYPLPSRKILDFARQVENI
                     FVIEENDPFIENHVKALGIRVIGKEHLPLCGELNSEMIATRLLGEKASGCEIAEEKAV
                     ARPPILCSGCPHRGVFYELSLLKDTVITGDIGCYTLGSAEPLNAMDTVLCMGAGISAA
                     IGFERAFQKIGRTSRVIGVIGDSTFFHSGITGLADAVFNRSQITVFILDNRSTAMTGH
                     QNNHGTGLRLSGKPAREIEIGEIVKALGVQHFYEINPNDLAGLKALLVKTAALEGPVV
                     ILAQWPCALLKELPPQDRSKFELPDHTYTVQADKCKKCKKCLALGCPSISFVPGIGSR
                     IDESTCKGCSLCYQVCPFHAIQKSGDRHV"
     gene            5684..9199
                     /gene="nifJ"
                     /locus_tag="PKM49_05470"
     CDS             5684..9199
                     /gene="nifJ"
                     /locus_tag="PKM49_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNY82297.1"
                     /translation="MGKKMVTIDGNYAAAHVAYAYSEVAAIYPITPSSTMGELIDQWA
                     AEGRKNMFGHIVKVSELQSEAGASGSVHGALQGGSLTSTYTASQGLLLMIPNMNKISG
                     ELLPAVFHVSARTVATHALSIFGDHSDVMHCRATGFALLASSGVQEVHDMAIVAHLSA
                     LDSSVPFLHFFDGFRTSSEIQKVELNDYEDLAKLINRDRIRAFKEKALRPERPVTRGT
                     AQNPDIFFQSREAANHYFDRVPFIVEENMKKVGQLTGRHYNLFDYVGAPDADRIIIAM
                     GSGTDVVEETMRYLNAKGEKVGVLKVRLYRPFSVQHLLAAIPMTVKHIAVLDRTKEPG
                     SAGEPLYEDVKFAFYGKENAPHIVGGRYGLSSKDFTPAQAKAVFDNLKGMQPKDHFTV
                     GIVDDVNFTSLPVEDISTEDPSVKRCVFWGFGSDGTVGANKNSIKIIGDHTDMYAQGY
                     FAYDSKKSGGVTISHLRFGPKPINSPYLIQEADFVACHRPAYVRIYDMVRYLKKGGTF
                     LMNSSWTLEEMERELPNKMKRSLARKQAKFYNIDAFKLAESIGMGEHINTIMQASFFK
                     LADIIPFEEAQKYMKQAIEKTYARKGQEVLDKNWEAVDVGASGLVEIPIPEAWADLPD
                     EELAPTCKPAFVTEVAEVMNRQEGNDLPVSAFSLDGTFPCGTTQYEKRGIAVKIPQWL
                     SDKCIQCNQCVMVCPHAVIRPILLTNEEAASAPGTLKALTPNHPKIKNDYKFTLQISG
                     LDCTGCGNCADVCPKDALEMVDFETAIELNQKNFTFTNEIPVKNPLGDAAWNTALFNQ
                     PLFEYHGACGGCGETPYLRLLTQLFGERMVIANATGCSSIYGGSAPSTPYCTNAEGKG
                     PAWANSLFEDNAEYGYGIALSITTTRDKIAMLMEDAIQNGLDEETKAAFEAWVAKRND
                     AEETKKMDKALRSALVLALSKASGPNKQLLNEIYELKDYFVKKSVWIIGGDGWAYDIG
                     YGGLDHVLASGEDINVLVLDTELYSNTGGQASKATPTAAIAKFAAAGKPGKKKELGLM
                     MTTYGYVYVAQIALGASMNQAFKAFKEAESYKGPSIIIAYAPCINHGLKAGMGFSIEE
                     EKKATACGYWPIWRYDPRLEEAGKNPFQLDGPKELNGKFQEFLQGEVRFSSLAQMFPE
                     RAEWLYKKAENDMIQRFNRYKKMAE"
     gene            9478..>10033
                     /locus_tag="PKM49_05475"
     CDS             9478..>10033
                     /locus_tag="PKM49_05475"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY82298.1"
                     /translation="MKKLPTGIQDYKYLREYDYIYVDKTKYLFDMINRGKVYFLSRPR
                     RFGKSLTLSTLKYLFLGEKELFKHTWIENRWDWKAYPVVHISLTETDTSTPETLQESL
                     LNLLEDTYKTFGLTLDSRLLKVAFRRFFSSVYEKTGERLVLLIDEYDKPILDHLHEPE
                     IAQKHRDLLRTFYSTIKDADPYLRF"
BASE COUNT         2402 a         2813 c         2569 g         2249 t
ORIGIN      
        1 gaccaaagag gcgtcgaagg cgctaaggtg attggatgcg atcatatacc ctcgatcttt
       61 tggaaggttc tcaaacccat cgatctccgg cttcatacca aaccaaagat aacttcggcg
      121 ggagatctcc acccctttgc tccgggcgta gacagcggcc gcctcttcac cgcggcgttt
      181 ttccagccag cggatacgcc aggtgtaata gaggctgtac aaaaggtagt agagcataat
      241 gcacgtataa tacgcgaggt atccaatggc cttaaacggg aagaaaatat aattcattgt
      301 ttatgtttct ccatagtttt aggtttattc ccagcttctc aaacgaagta gcgcgccgat
      361 tttcgcgatc atcgtcatcg caaactccct tatccccgag cccgcgcaca ctgtttgatg
      421 gtccacggtc ttcgaaactc gagttttcaa gtccgctaag gcgttggcgc ttttatccaa
      481 agtttcccta tcgagagttt cttgaaagaa gagcgggaac attccggcca actctgccgc
      541 tttcaggagg agcgcgcccc aactttcgaa ctcgaccaac cagggccgaa cctctcgaag
      601 ccacgcaagg ggcatcgtcg ttttaagcgt ctgcactgct tcgttgagcg tcaaggcgtg
      661 ttgttgtaat agagaggccg cttgcgcgat cgacccggat cgcgcggaag aagaataggc
      721 gtctatccaa cgatcagaga ccatttcctc tccttttggg ttgagcggac tgatcaggtt
      781 catctccgca aagagcttca gactctgagc gcagtgaggc gcgaaagctt ccaacgcgct
      841 ttcccaagcg gatagggggt catatgtcgc ggggtctttc aaataggcgg cgatgctcgt
      901 caaaacggga atagaggcca tcgcttggtt cattgggttg atcaaaaatc cgcgactgac
      961 cgtgggcagt ctcacgtcac ggccggtgta aggcccgatg tggagctcgc aagccattga
     1021 gccatcattc acagggtagt tgtcccaata gaccaccttc cttcgaaggg ttcgactcac
     1081 cgtttcggcg tcttcgaacg gaattgttcg cgagcagacc tgcggtcccg tccaaagcgc
     1141 gtcgatgtcg ggatgcaacc ctgagccaat ctctttgaga tatggggtct ggccattccc
     1201 acagtactgg gtcgggcaga acaggaaaga ggttaccgcg gtcaagtttt tcaaacgctc
     1261 atagacgtgg ttcgtaaaaa aaacctgcgc gtcggccaaa ctggcaaagg tgccccggtc
     1321 cgcctccttt tgcaaggata aggggatgtc gtccaagaac agacaaaaat ccgagacgcc
     1381 gatagccgcg aacgagaggt atttccggac tagcgcatcg atctgtccaa aatcggaata
     1441 ggtcaacgtt aaccccggac tcaaggctaa cgccacgcca acgcccgacc ccttcccggc
     1501 ttcgaccagc gcgttgaact cccggataaa ctcctgcggg tacggtgttt cccaacggtg
     1561 ccggtgcaac aggtcattct tgggggcata gacgtacaga ttcatttcga gtcgggccat
     1621 ttcttgaatg atttgcgtcc gttcttccat cgtccaagga ggtccgtaaa acccctctac
     1681 gacgcctctg atctgaaaac cgctcattcc actcgctcct ttgctatgtt tgggacattt
     1741 cttagagaac gcggttcggt tttcatataa aagatagtcc gcgcccacgc acccgggaaa
     1801 aaacaaggcg tacgttaacg tacgccttgt cctcgaccat tcataccgct tggttaagtc
     1861 cgttcgctca aaacgagcga tcaaacgcgg aaccgcttta aaggtgttcc cactccgcct
     1921 cgaaggtgcc gccagtcgag gcagccccga gcgttcgggt gaccgttccg gtcatctttt
     1981 tttggtagcg gagcaccata tcatcgagct cggccgaaaa atgatagacg tttcctgttg
     2041 cggtgtgcgt aaaagaaatc tccatcttgg ccggtatgcc gaacttcgcg ccggaaatcg
     2101 tccccgtaat cgcaaacccg ttaaaggggg ctccatgcgt catgccgccc aaaccgccaa
     2161 agttgtcgga ccagatatgg atgagttgcg tcgctaaacc cggtattgtc gttgtgaagg
     2221 tccaataacc cgtgatgttt gggtaagctt gtagcgcgac ataacaaccg gatgtcgcga
     2281 acgtcaatac cagtaccatc aaagaaatca ctaagagttt tttcatccta aacgcctccc
     2341 ctggatgtgt tgttcaagcc cgtttactgc ttgacggctt caaaacgatt tccgtctttc
     2401 tccacagttc ctacgatctt cggagaggtt ctgtcgatct tgccgttcag cacttgatcg
     2461 ccaacgagaa tgttgatgtt gccttggcta tccgcgttat agcggaagtc gtggtcgaca
     2521 ccgtcgatag aaaacttgcc ggtcgtgaaa ttcataacga tgccatcggg gtcgggcgct
     2581 cgcgcttccc ctaacagaaa gccccaatcg aaggtcacag cccacgggcc tcccaaccag
     2641 gagaacggcc atcccaccgt gacactacag ttggtcataa ataaagccat caataaaagc
     2701 aagcttaaca gcagtacgat acgaactttt ttcatactca cacctcctga aagatttctc
     2761 cctttgtata ttctacactt attttgcaaa aaaagcaagg aagacgctag aacggatatt
     2821 taccggctgt tagctcaatt cttctgtgtg aaaccgtatc ctttagctaa agccgtttgg
     2881 tttttttcat ggaaccgctc tttgatcaac ttcatcaata cagcctccgt ttgatcgtac
     2941 ccgatcccca gagttttcgc caaaacgccc agtaatacgg tgttttgata gctcgtcccg
     3001 ccgatttcgg ctgcccattc ggttaccggc acccgaagcg cccgtccggt cttttcgatc
     3061 tcgtcgtcta acccctgagg atactctttt ttcgcggaaa agatcggcgc cgaagggatc
     3121 atcgtatcgg cgtagatcac agtcccgccc gccttgaggt agggaaggaa ccgataggtc
     3181 tccatcttct cgaacccgag taaaatatcc gcgcctccgt gacccacaaa aggcgagtac
     3241 accttttttc caaaccggat ttgtgtggag acggagcccc cccgttgggc cattccgtgg
     3301 acctcggaca tcttgacatc gtatccattt tcgaccagca gctcagataa caccttggag
     3361 acgaggagta taccctgtcc tcccactccg accagtacga cggtttttgt ttcagacatg
     3421 ccgatcacca ctcttctgta tcgcgtggaa gggacagacc tgataacaca aagaacagcc
     3481 tttgcaggtg ctttcgtcta ttctggaacc gatgcccggg acgaacgaga tcgacggaca
     3541 ccccagagcc aagcactttt tgcatttttt acatttgtcc gcctgcaccg tgtaggtgtg
     3601 gtcaggaagc tcgaacttcg acctgtcctg aggaggaagt tccttcaaca gggcgcaagg
     3661 ccattgggcg aggatcacga caggtccctc cagcgcggcg gttttgacga gaagcgcctt
     3721 caatccggcc agatcgttgg ggttgatctc atagaagtgc tggactccca gagctttgac
     3781 gatctcaccg atctcgatct cgcgcgccgg tttcccactc aagcgaagcc ccgtaccgtg
     3841 gttgttctgg tgccccgtca tcgcggtcga ccgattatcc aagatgaaga cggtgatctg
     3901 ggaacggttg aacacggcat cggcgagccc ggtgatcccg gaatgaaaaa aagtggagtc
     3961 gccgatcacg ccaatcacgc ggctcgttct gccgattttt tgaaaagccc tttcaaatcc
     4021 aatggcggcg ctaatcccgg cgcccataca cagaacggtg tccatcgcat tcaacggctc
     4081 cgcgctgcca agggtgtaac agccgatgtc tccggtgatc acggtgtctt tcaataggct
     4141 cagctcataa aacacacccc ggtgagggca tccagaacat aggatgggcg ggcgagcgac
     4201 ggctttctcc tccgcgatct cacaacccga agccttctcc cccaacaacc gcgtcgcgat
     4261 catttcgctg ttgagttcgc cgcataaggg caggtgttct ttcccaatca ctcgaatacc
     4321 caaagccttc acatggttct cgataaacgg gtcgttctct tcgatgacga agatgttttc
     4381 gacctgtcgc gcgaaatcga ggatcttccg cgaagggagc ggataggtga acccgagttt
     4441 gagataggtc gcgcgagacc cgaagacctc cctcgcgtgt tgataagcga taccgctggt
     4501 gatgacgccg gtcttcgaat cccccggctc gatgaagttg agctccgttt cgttgctcca
     4561 ctcttccaat tcctttagtt tcagttctag ttcggcgtga tggacacggc agcgcgcggg
     4621 agtggcaacg aaccgtgaga gatctcgtct ataggtggtg ggtggtttct cgattctttc
     4681 gcctaactcc accagccctt tactgtggca gacacgtgtc gtcatccgga acagcacggg
     4741 gatccggtgt cgttcgctca cttcgaaggc cattttcatc atatctttgg cctcttgact
     4801 atcggacggt tcgaagagtg ggactttggc gtgcacggcg tagtgtcggt tgtcttgttc
     4861 cgtttgggaa gagtgtaaac cggggtcgtc ggcggtaagg atgacgcacc cgccctctac
     4921 acccaagtag gcgccggaaa agagggggtc cgcggcgaca ttgaggccga cgtttttcat
     4981 cgtcgcgagg gatctcaaac ccgcgaaagc cgaaccgaaa acgatctcga gcgcgacttt
     5041 ttcattgatc gaccactcgg catcgatctc ccgataccgc gccaaagact ccagaatctc
     5101 cgtgctgggc gtccccgggt agccacacgc cacctggaca ccggcttcat aagcccccct
     5161 cgcgcaggct tcatttcccg atagcaaaac tttccccatc tttcacctct tccatagttc
     5221 ttttatgctc aatcaccgag attatagcac atgtaggcaa gggattattc ctttcgcggg
     5281 agcggttaga acgccgaacg cccgggaacg ctctgggaac gtctgggaac gccgaccttt
     5341 gtgggtcatc gcggagtaac caaagggacc ccgcgatacc ccgcttctat gctttttact
     5401 ccacgatggg ctcgtataaa tgcgtatcgc gccgtcgcga aaagaagaag cgcttacgcc
     5461 gcgatgctcg tgtttgttat cgcagggtaa gtgattttta cagcgccacg acgccgcgct
     5521 tgggaactcc aaggcattcc cagttaaact atgtgaaaca taaactttgc gctccaaaaa
     5581 cgatgacgac cgcgtgcgcg tcaagtccca ataaccgttt attatgctat aatctaatcg
     5641 aagctataag gactaccaaa atactagaaa ggatggatga attgtgggca agaaaatggt
     5701 taccattgac gggaactacg ccgcggcgca tgtcgcttac gcgtattcgg aagtcgccgc
     5761 gatctacccg atcacccctt cttccacgat gggagagctg atcgatcagt gggccgcgga
     5821 aggaaggaag aacatgttcg gacatatcgt aaaggtatcc gaactccaat cggaggcagg
     5881 cgcttccggt tccgtgcacg gcgcgttaca gggagggtct ctcacctcga cctacaccgc
     5941 ctcgcaaggt ttacttctca tgatccccaa tatgaataag atttcgggag agctgcttcc
     6001 cgcggttttc cacgtatccg ccagaaccgt ggccacccac gccctgtcga tcttcggaga
     6061 ccattccgac gtgatgcact gtcgcgcgac cgggtttgcg ctattggcct cctcgggagt
     6121 ccaagaggtc cacgatatgg cgatcgttgc gcatctctcg gcgttagact cctccgtacc
     6181 gttcctacac tttttcgatg gattccgcac ttcctcggag attcagaagg tcgaattgaa
     6241 cgactacgaa gacctcgcca aactgatcaa tcgcgatcgc atacgcgctt tcaaagaaaa
     6301 agcgttaagg ccggaacgcc cggtgacccg cggtaccgct cagaacccgg acatcttctt
     6361 ccagagccgc gaagccgcca accattattt cgaccgagtt ccgttcatcg ttgaggagaa
     6421 tatgaaaaaa gtgggccagt tgaccggtcg ccactacaac ctcttcgatt atgtgggcgc
     6481 gcccgacgcc gaccgcatca tcatcgcgat ggggtccggc accgatgtcg tcgaagagac
     6541 gatgcgctac ctgaacgcaa agggcgagaa ggtcggggtg ttaaaagtta ggctgtacag
     6601 accgttcagc gttcagcacc tcctcgcggc gattccgatg acggttaaac acatcgcggt
     6661 tttggaccga accaaagaac ccggttccgc cggtgaacct ctgtatgaag acgtgaaatt
     6721 cgccttctac ggaaaagaaa acgcccccca tatcgtcggt ggccgttatg ggctcagctc
     6781 gaaggacttc acgcccgcgc aggcgaaggc cgttttcgat aacctgaaag gaatgcaacc
     6841 gaaagaccac ttcactgtcg gtatcgttga cgacgtcaat ttcacctccc ttccggttga
     6901 agatatctcc accgaggatc cttcggtcaa acgctgcgtc ttctggggct tcggctctga
     6961 cggaaccgtc ggcgcaaaca agaactccat caagatcatc ggtgaccaca ccgatatgta
     7021 cgctcagggc tatttcgcct acgactccaa aaagtccgga ggcgtgacga tctcccattt
     7081 gcgctttggg ccgaaaccga tcaatagccc ctatctgata caggaagcgg atttcgtcgc
     7141 ttgccatcgc cccgcttacg tccgcatata tgatatggtc cgatacctga aaaaaggcgg
     7201 caccttcttg atgaactcca gctggacgct tgaagagatg gaacgcgagc ttcccaacaa
     7261 gatgaaacgc tctctggctc gcaagcaggc gaagttctac aatatcgacg ccttcaaatt
     7321 ggcggaatca atcggtatgg gcgaacacat caacaccatc atgcaggcct ccttcttcaa
     7381 actggccgat atcatcccct tcgaagaagc gcaaaagtat atgaaacagg caatcgagaa
     7441 gacctatgcc agaaaagggc aagaggttct ggataagaac tgggaagccg tcgatgtcgg
     7501 cgccagcggt ttggtagaaa taccgattcc ggaagcttgg gcggacttgc cggacgagga
     7561 gctcgcgccc acgtgcaaac ccgccttcgt gaccgaagtc gccgaggtga tgaatcgcca
     7621 ggaaggtaac gacctgcccg tttccgcctt ctctctggat gggaccttcc cctgcggaac
     7681 cacccaatac gaaaagcgcg gtatcgccgt caagataccc caatggcttt cggataagtg
     7741 cattcaatgc aaccagtgtg tgatggtctg tccccacgcg gttatccgcc ctatcctgtt
     7801 gacgaacgaa gaggccgcat ccgccccggg cacgttgaaa gcgctcacac ccaaccatcc
     7861 gaagatcaag aacgattaca agtttacgct gcagatcagc gggctcgact gcactggatg
     7921 tggcaactgc gccgacgtct gtccgaaaga cgctctcgaa atggtagatt tcgaaaccgc
     7981 catcgaactg aatcagaaga attttacgtt tacaaacgaa atcccggtga aaaacccgtt
     8041 aggcgacgcg gcgtggaaca cggccttgtt caatcagccg ctctttgaat accacggcgc
     8101 gtgcggcggc tgtggagaaa ccccgtacct gcgattgctg acccagcttt tcggagaacg
     8161 gatggtcatc gccaacgcga ccggctgttc ttccatctac ggcggaagcg ccccctccac
     8221 gccgtactgt acgaacgcgg aaggaaaagg acccgcttgg gcgaattcgc tgttcgaaga
     8281 taacgccgaa tacggatacg gtatcgcgct ctccatcacc actacgcgtg acaagatcgc
     8341 catgctgatg gaagacgcta tccagaacgg actggatgaa gagacgaaag cggccttcga
     8401 ggcttgggtt gccaagcgca acgacgccga ggaaaccaaa aaaatggata aagcgcttcg
     8461 ctctgcgctc gtgctcgcgc tgtctaaggc ctccggtcca aacaaacagc tgttgaacga
     8521 gatatatgaa ctgaaagact acttcgtgaa gaagtccgta tggatcatcg gtggagacgg
     8581 ttgggcctat gatatcggat acggcggctt ggaccacgtg ttggcctccg gcgaggatat
     8641 caacgtgctc gtgctggaca ccgaactcta ctccaacacg ggcggacagg cttcaaaagc
     8701 gacacccact gctgcgatcg ccaaattcgc cgccgccggg aagcccggaa aaaagaaaga
     8761 actcgggtta atgatgacca cctacggcta cgtttacgtc gcgcagattg cgttgggcgc
     8821 cagcatgaac caagccttca aagccttcaa agaggctgag tcatacaaag ggccgtcgat
     8881 catcatcgcc tatgcgcctt gtatcaatca cggtttgaag gcgggtatgg gcttcagcat
     8941 cgaagaggaa aagaaagcga ccgcgtgcgg ctactggccg atttggagat acgacccccg
     9001 tttggaagag gctggcaaaa acccgttcca attagacggt ccgaaagaac tcaacggcaa
     9061 gttccaggag tttttacaag gtgaagtgcg gttttcttca ctcgcccaga tgttccccga
     9121 acgggctgag tggctctaca agaaagccga aaacgacatg atccagcgct tcaaccgcta
     9181 caaaaagatg gcggaataac ggcttagaag atttcagcga cccttcggct ttcacgagca
     9241 gaagggtgac ttagggcggc gcgctcttta cgccgccctt ttggtaacgc cctgggaacg
     9301 ccggacttca gtccggcgtg tcttctacgc tttcatcttt ctccgtgtcc tctgcgttct
     9361 ccgtgcgaac ccattttttg tcattcccag cctgcccccg cgggtgattt tacaagtcga
     9421 agctcttcat gctatactct ctcatagggc tactaacgaa cacaaatgcg agggcaaatg
     9481 aaaaagctac cgaccggaat ccaggactat aaatacctta gggaatacga ctatatttac
     9541 gtggacaaga cgaagtacct tttcgatatg atcaatcgcg ggaaggtcta ttttctctca
     9601 cgcccacgtc gattcgggaa atcgctgacg ctctcgacgc tgaagtatct ctttctcggg
     9661 gagaaagaac tattcaaaca cacctggata gaaaaccgat gggactggaa agcttatccg
     9721 gtagtacata tcagtttgac agaaacagac acgtctactc cggaaacgct acaagaaagc
     9781 ttattgaact tgctcgaaga tacatataag accttcggat tgacgttgga cagtcgctta
     9841 ctgaaagtcg cctttcgccg tttcttctct tcagtttacg agaaaaccgg ggagcgcttg
     9901 gttttactca tcgacgaata cgacaaaccg atactcgacc acttgcacga accggaaatt
     9961 gcgcaaaagc accgagacct gcttcggacg ttctattcca cgatcaagga cgcggacccg
    10021 tatctgcggt tcg
//
LOCUS       DAOPXN010000072         9889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6894_length_9889_cov_4.866484, whole genome shotgun sequence.
ACCESSION   DAOPXN010000072 DAOPXN010000000
VERSION     DAOPXN010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6894_length_9889_cov_4.866484"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PKM49_05480"
     CDS             complement(<1..1051)
                     /locus_tag="PKM49_05480"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNY82299.1"
                     /translation="MRRTVPLSGPDIGEEEVRAVEGVLRSTILSIGPKVQEFERRMAE
                     YSGLRFCVAVNSGTSALMLIMKAAGVKAGDRYLTTPFSFVTSSNILLYENAEPVFVDI
                     DPLTFNMAPRALEERYATDPAKETIKGIVGVDIFAQPLDWDPILNFSRKHGLQVIEDS
                     CEALGSSYKGRSCGTFGIAGAFAFYPNKQMTTGEGGVVVTDDEEIYLLCRSMRNQGRG
                     VAENWLEHVRLGYNFRMDELSAALGVEQVKKLDVFIRKRNTVAERYAKLLAGVEGVRA
                     PVIAPFTTRVSWFVYVMTLAEGIDRDRLIVYLQERGVQSRNYFTPIHLQPFYKERFGY
                     REGMLPITENLSKRTL"
     gene            complement(1051..2052)
                     /gene="plsX"
                     /locus_tag="PKM49_05485"
     CDS             complement(1051..2052)
                     /gene="plsX"
                     /locus_tag="PKM49_05485"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HNY82300.1"
                     /translation="MVTIALDCMGGDYAPKSVVDGIMKYLESENEEVSFLLTGQEEKI
                     KPCFPHGEVPERVRIIPSPEVFAMDEKPSMILKRPETSLHKAAALVRDKEADALVSAG
                     NTGGLLAAATFLIGRIKGVKRPALITPIPNLHRDVLLLDSGATVECSKEVLIGFSKMS
                     IAFLQVLGAVDYKIGLLNVGTEEDKGTQDLKQANVILKEQLGKNYQGFVEGRDISLGP
                     ADVIITDGFSGNIALKTIEGTAKLIMETLKSEIKKGGLRTKLGALLLKPALKALKHRF
                     DPHRVGGAFILGVNAPVVKAHGNSDGYAISNAIRVAIDGVKKQLVKKIALQYAQGEA"
     gene            complement(2168..3295)
                     /locus_tag="PKM49_05490"
     CDS             complement(2168..3295)
                     /locus_tag="PKM49_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82301.1"
                     /translation="MVFIAAFSHFFTDFFSAFFKPMAPYFMSLYQIDSSTFGSTLGII
                     GSCASLLQIFFGLLFDRTKRDGWLLFSLLMFQLALLGLLVFIRHYALLMLLIFLSILI
                     NSAYHPLGAGMAGRTGKARAVAIFSIFGTFGSALGPIFITAFSSRFSFRDVAWIALAS
                     GLVLTLLAPKFSPIKKEERARQRFPTKSLFVLLMPCFNAVVLRTIVLDQFHILVPIYF
                     DGLKAPLTLGGVVLAVGMLAGLVTNYAGSRWILKVGVQRINLVGFLGMGIGGLLFLLA
                     PGYVAKTLMFALFDSAGFLTMSANVVQAQALVPNNLAFASSVTMGLAWAIGGYIGAFI
                     SVFLGNRVTMLLALLSAFSLGVALLYLFLIRNDRTRRPIAV"
     gene            complement(3304..4158)
                     /gene="ylqF"
                     /locus_tag="PKM49_05495"
     CDS             complement(3304..4158)
                     /gene="ylqF"
                     /locus_tag="PKM49_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNY82302.1"
                     /translation="MATEKTWYPGHIEKAKRVIQENLKAVQVVVEMADARIPYSGRAY
                     EWKSLFHNKKTVILLSKADLADPGQTDRWISFYEKAGMVAFAMNLKGNPGSIRKGFVS
                     KLTDIAREDRFQRYMIVGSPNVGKSTLVNVLLGKKKVRVGRTPGLTRGIQWVNVTDQL
                     MLMDTPGILYRHLFSKHVRHKLLLAGCIKASESEAEEALEYAFLWLKERYPNAYRTIT
                     GQSAEGPFEYEELLRLVGLRRGYLQKENRVDRSKVVDFLLASFQNGTFGALTYETPDS
                     LDIASDSG"
     gene            complement(4208..5005)
                     /gene="lepB"
                     /locus_tag="PKM49_05500"
     CDS             complement(4208..5005)
                     /gene="lepB"
                     /locus_tag="PKM49_05500"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNY82303.1"
                     /translation="MGQKAAHEAWDWFKAFLYAAVAVVIVRLFVFETMMVPTGSMIPT
                     IVPLDRLFVERVTYSTKEPDYGEIVVFWTPFVDKEAQKQLRAFDKFMDIFSPAEYRGH
                     VKYVKRLVGKPGDVLELTPNLSGGGYSLLVNGQTPERLKGVTYQKEGIFKDPRFYHQM
                     AYPSEYPWLTANMKSFFEFYNKALDYTAAYREVFDGLEIGDYAWKVADSDRIQIRVPE
                     GGYFFMGDNSPESLDSRFFGFVPKKNIVGGPLLRIWPLNRFGPINKQ"
     gene            complement(5086..5442)
                     /gene="rplS"
                     /locus_tag="PKM49_05505"
     CDS             complement(5086..5442)
                     /gene="rplS"
                     /locus_tag="PKM49_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNY82304.1"
                     /translation="MSNAFIRAMAQQDYRELPPFNVGDTVRIFQRIKEGNKERTQKFE
                     GIVIQKRGDGLNKTFTVRKIGANGIGVEKIYPYHLPAIEKVEVVKKGKVRRAKLYYIR
                     KIVGVVKIKEKRQKSQ"
     gene            complement(5493..6059)
                     /locus_tag="PKM49_05510"
     CDS             complement(5493..6059)
                     /locus_tag="PKM49_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNY82305.1"
                     /translation="MLDKIYVLLVHYPCLGRDQQIIATAVTNVDIHDIARVCRTYNVK
                     KYYMVTNLPAQQEIVRRVVQFWTEGSGIRYNPNRSEALNYVILKPYLEDALEEIERIE
                     NQKPVLIFTSAKKRPSSMTYDEGQRLLLTETRPIVLLFGTGWGLPAEVLSLCDYDLEP
                     VRPGGSFNHLSVRSAVSIILDRLIRENY"
     gene            complement(6049..6792)
                     /gene="trmD"
                     /locus_tag="PKM49_05515"
     CDS             complement(6049..6792)
                     /gene="trmD"
                     /locus_tag="PKM49_05515"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNY82306.1"
                     /translation="MKFAVVSIFPEMIEAFLSRGIVSRARANKTLEIEVIQLRDYTHD
                     AHRTTDDYVYGGGGGMVMKPAPVFECIDDYATRNGPFKVVFPSPQGQIFDSARAEELA
                     RQENLLFLCGRYEGIDERIMTLVDYELSIGSFVVSGGEIPALLMIDAISRFVPGVVGN
                     ERSVKQDSFYNGLLDHANWTRPAVYRGLEVPAVYVNGHHQEIEKARKRDSILRTILKH
                     PKLFLEKEIGPEEKNAIIEIIRELSTDAG"
     gene            complement(6801..7403)
                     /gene="rimM"
                     /locus_tag="PKM49_05520"
     CDS             complement(6801..7403)
                     /gene="rimM"
                     /locus_tag="PKM49_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNY82307.1"
                     /translation="MIHQIEKILEHSVPIARLLGPHGLRGEIKATMLANYPGVFETGK
                     EFFLFHSKKRSHLRCTLDAFHRRGDRMILRFRNYDHIDLAKELEGYEVYLDLCDLPLL
                     KAGEYYFFQLLGASVYDEQGQRLGVVEDVIETGSADVLSIRKPFSGPGDPPKDSELLV
                     PMAKDFLVSMDLEQKRLVIRPPVYMAVKDDETDAGSDEGF"
     gene            complement(7411..7656)
                     /locus_tag="PKM49_05525"
     CDS             complement(7411..7656)
                     /locus_tag="PKM49_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNY82308.1"
                     /translation="MKDALEFIIKGIVKHPEDVRVSRSDEKTPDSPPSELYDLRLHVE
                     DVGQVIGKDGRTIKSINILLNALAGQKEEKFILRVQR"
     gene            complement(7670..7981)
                     /gene="rpsP"
                     /locus_tag="PKM49_05530"
     CDS             complement(7670..7981)
                     /gene="rpsP"
                     /locus_tag="PKM49_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNY82309.1"
                     /translation="MVKIRLTRLGKRKRPFYRVVVIEANKRRDGRYIESIGFYDPIND
                     QNLSKLDTEKALAWLLKGAQPTDTVRDILSKHGVMESFDREKRKREASSHPSPETPEQ
                     K"
     gene            complement(8026..9336)
                     /gene="ffh"
                     /locus_tag="PKM49_05535"
     CDS             complement(8026..9336)
                     /gene="ffh"
                     /locus_tag="PKM49_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNY82310.1"
                     /translation="MFESLQEKLEKAFKSLRGQGKISEKNIQEAIRMVKLSLLEADVN
                     YKVVKDFIEKVKEKAIGAEVFNSLTPDQQFIKIVRDELTALMGEKHEKLRFTQKPSPI
                     MLVGLQGSGKTTTGAKLARIIKSEGRKVLLVAADIYRPAAIDQLEILGAEIGVTVFSG
                     DRRHVDKICQEGMKLAIGSLYDVVIFDTAGRLHINEEMMREVEKVREIVKPDEVLMVL
                     DAMIGQDAVQTAKTFNDRLAVTGFVLTKMDGDARGGVALSIRSVTGKPIKFIGVSEKI
                     DGLEAFYPDRLSSRILGLGDVLSLIEKVEQTIDEKKAKELEKKLRKADFTFNDFLEQI
                     RQIQKMGSLRSILEMLPGGSKLKGVTVDEKQIKHVEAIICSMTKKERANPKLINYSRK
                     KRIALGSGRPLPEVTKLLDNFENMKKMVKKIGKMGPKGLSRLPF"
BASE COUNT         2222 a         2779 c         2431 g         2457 t
ORIGIN      
        1 ccagcgtccg tttcgacagg ttctccgtga tcggcaacat cccttctcgg tacccaaacc
       61 gttctttata gaaaggctga aggtgtatgg gggtgaaata gttccgactc tgaacacccc
      121 gttcctgtag atagacgatg agacggtctc gatcgattcc ttccgccagc gtcatgacgt
      181 agacgaacca actgacgcgc gtggtgaatg gcgcgatcac cggcgctcga acgccttcca
      241 cgccggctaa gagcttggcg tagcgctcag cgacggtgtt ccgtttccgg ataaatacgt
      301 cgagcttctt cacctgctcg actcctaaag ccgcgctcag ttcatccatc cgaaaattgt
      361 atccgagccg gacgtgttcc aaccagttct cggctacccc acggccttgg tttctcatcg
      421 accgacaaag caggtatatc tcctcgtcgt cggtgacaac taccccaccc tctccggtgg
      481 tcatctgctt gttcggataa aaagcgaagg ccccggcgat cccgaaggtg ccgcaactcc
      541 ttcctttata ggaagagccc agcgcctcgc aagagtcttc tatcacttgt aaaccgtgtt
      601 tgcgagaaaa attgaggatc ggatcccagt cgagcggttg ggcgaagata tcgaccccca
      661 cgatcccctt gatcgtctcc ttggcgggat ccgtcgcata cctttcctcg agcgcgcggg
      721 gggccatatt gaaggtcaac ggatcgatat cgacgaaaac aggctccgcg ttctcgtaca
      781 gaaggatatt gctggaggtg acgaaagaga agggagtcgt gagatagcga tcgccggctt
      841 tcacccccgc cgctttcata atcagcatca acgcgctggt tccgctgttc accgccacac
      901 agaagcgaag cccggagtat tcggccatac gccgttcgaa ctcctgaacc ttaggcccga
      961 tgctgagaat ggtcgaacga aggactcctt caaccgcgcg tacctcttct tccccgatat
     1021 cggggccgga taatggaacg gttcttctca tcacgcttcc ccctgagcat actgaagtgc
     1081 gattttcttg accaattgtt tcttcacccc gtctatggcc acgcggatcg cgtttgagat
     1141 cgcatagccg tcggaattcc catgcgcctt gacgaccggc gcgttcacgc ccaagatgaa
     1201 tgcgcccccg acgcggtgcg ggtcgaaacg gtgttttaac gctttgaggg cgggcttcag
     1261 caataaggcg cccagcttgg ttctcaatcc gcccttttta atctctgact tcagggtttc
     1321 cataatcagc ttcgcagtgc cttctatcgt ttttaaggcg atattcccgc tgaatccgtc
     1381 ggtgatgatg acgtctgccg gaccaagact gatatcccgg ccctctacga aaccctggta
     1441 gtttttgccc aactgttctt tgagtatcac attcgcctgt ttcaaatcct gggttccttt
     1501 atcctcttcg gtgcccacat tgagcagccc gatcttgtaa tcgaccgcgc cgagcacttg
     1561 taaaaaagcg atgctcattt tggaaaaccc aatgagcact tccttggagc attctaccgt
     1621 cgccccgctg tccaacaaca acacatcgcg gtgcaagtta gggatgggcg tgatcaaagc
     1681 cgggcgcttc actcccttga tacggccgat caaaaaggtg gccgcggcga gcaagccgcc
     1741 ggtgttgcct gcgctgacca gcgcgtcggc ttctttatct cgaacgagcg cggctgcttt
     1801 atggagagat gtttccggtc gtttcaatat catggatggt ttttcatcca tagcgaaaac
     1861 ttcgggcgac ggaatgatcc gaacgcgttc gggaacctcc ccgtgaggaa agcagggctt
     1921 gattttctct tcctgaccgg tgagtaagaa agagacctct tcgttttcac tctctagata
     1981 tttcattatc ccgtccacaa cggatttggg ggcgtagtcg cctcccatgc agtccagcgc
     2041 aatcgttacc atttttcttt cctccactgc gaatgcgctc tcgtctttcc gttgaacgaa
     2101 cgcattcgat tattcgctat ccagatttcc ttatctattt tacacgtttt ttttctcata
     2161 agtgcgctta aaccgcgatc ggccttcgtg ttcgatcgtt tcggatcaaa aacaagtata
     2221 ggagcgccac acccaaagaa aaggccgaca acagggcgag tagcatagtc acgcgattac
     2281 ctaaaaagac ggaaatgaat gccccgatgt aaccgccgat ggcccaagcc aaccccatcg
     2341 tcaccgagga ggcgaaagcg aggttgttcg gcaccagcgc ctgtgcttgc acgacattgg
     2401 ccgacatcgt taagaagccg gcagaatcga agagcgcaaa catcagggtt ttcgcgacgt
     2461 acccgggcgc gagaaggaat agaagacccc ctatacccat acccaagaat ccgacgaggt
     2521 tgatgcgctg aacgccgact ttcaagatcc aacgagaacc cgcgtaattc gtgaccaacc
     2581 cggccagcat cccgacggct aacacgacac ctccgagcgt taagggagct ttcagaccgt
     2641 cgaagtagat cggaaccaag atgtgaaact ggtcgaggac gatggtcctg agcacgacgg
     2701 cgttaaaaca cggcatcaac aatacgaaca gggacttggt cggaaaccgc tggcgcgccc
     2761 gctcttcttt ttttatcggc gaaaacttcg gagccaagag ggtgaggacc aggcctgagg
     2821 ccaatgcaat ccaggcgacg tcacgaaaag agaatcggct actgaacgcg gtgataaaaa
     2881 tcggccccag cgccgacccg aaggttccga agatcgaaaa gatagcgacg gcgcgcgctt
     2941 ttcccgttcg acccgccatt cccgcgccca acgggtggta agcggagttg atcaagatgg
     3001 ataggaagat caacagcatg aggagcgcgt agtgccggat aaaaactaaa aggccgagga
     3061 gcgcgagttg gaacatcaat agggagaaga ggagccaacc gtcgcgcttg gtcctatcga
     3121 ataggagccc gaaaaaaatc tgcaataggg acgcgcagga accgatgatc cccaaagtgc
     3181 tgccgaaagt cgaactgtct atttggtaca acgacataaa ataaggcgcc atgggcttga
     3241 agaaggcgct gaaaaaatcc gtaaaaaaat gagaaaacgc cgcgatgaaa accatagccc
     3301 tccttacccc gaatcggaag cgatgtcgag gctatccggg gtttcgtagg tcaaggcgcc
     3361 aaaggtgccg ttctgaaagg aggcgagcaa gaaatccacc accttcgatc ggtccacacg
     3421 gttctctttt tgcaggtacc cgcgtctgag gccaacgagt cgtaggagct cttcgtattc
     3481 gaagggcccc tcagcgcttt ggcccgtaat cgttcgatac gcgttggggt agcgctcttt
     3541 caaccagaga aacgcgtact ccaacgcttc ttcggcctcg ctctcggagg ctttgatgca
     3601 tccggccaac agcaatttat ggcgcacgtg cttactgaag agatgtcgat acaggatccc
     3661 gggggtatcc atcaacatca gttgatcggt gacgttcacc cactgaatcc cgcgcgtgag
     3721 gcctggggta cggccgacac gcaccttctt ttttcccagg aggacgttga caagcgtgga
     3781 cttcccgacg ttggggctac cgacgatcat ataccgttga aaccggtcct ctctagcgat
     3841 gtcggtcaac ttcgacacga agccctttcg tatgcttccg gggtttcctt tcaggttcat
     3901 cgcgaaggcc accatacctg ccttttcgta gaacgagatc catcggtccg tttgacctgg
     3961 atcagccagg tccgccttcg agaggagaat gacggtcttc ttgttatgga atagggactt
     4021 ccattcatag gctcttccgg agtacggtat acgcgcgtcg gccatctcca cgacaacctg
     4081 aaccgccttc aggttctctt ggatgactct tttcgctttt tcgatgtgcc cgggatacca
     4141 cgttttttct gtcgccatcg tgatccatta aaaaaggtct cgccagaaaa ggcaagacct
     4201 ttctgcgtta ttgtttgttg atcggtccaa agcggttaag cggccaaatc cgcaatagag
     4261 ggccgccaac gatatttttc ttgggcacga atccaaaaaa gcggctatcc aaactttcgg
     4321 gagagttatc ccccataaag aaataacctc cttcgggcac ccgtatctgg atgcgatcgg
     4381 aatccgccac tttccaagcg taatcaccga tctccagccc gtcgaagact tcccgatagg
     4441 cagcggtata gtcgagcgct ttattataga attcgaaaaa actcttcata ttcgctgtca
     4501 accaagggta ttcgctcgga taggccattt gatgatagaa ccgagggtct ttgaagatcc
     4561 cttccttctg gtaagtgacg cctttcaacc gttcgggcgt ctgaccattg accagcagag
     4621 aatagccacc tccggacagg tttggcgtga gctcgagcac atctcccggt tttcctacca
     4681 accgcttgac gtatttcacg tgtcctcggt attccgccgg cgagaagatg tccataaact
     4741 tatcgaacgc acggagctgc ttttgggctt ctttgtccac gaagggggtc caaaagacca
     4801 cgatctctcc gtaatccggc tctttggtac tgtacgtgac tctctctaca aaaagacggt
     4861 ccaacggtac gatggtcggg atcatcgatc cggtgggaac catcatggtc tcgaagacga
     4921 atagcctaac gatcacgacg gcgacggcgg cgtataaaaa cgctttgaac cagtcccaag
     4981 cctcgtgcgc ggctttctgt cccatcgtct tcttcggagt cgacggattg cttttgggtt
     5041 tacccttcct ttccggtgtt ttcgccatag ccaacccctt tcggattact ggcttttctg
     5101 tcgcttctcc ttgatcttca cgacacccac gatctttcgg atatagtaaa gcttggcgcg
     5161 acgcactttc ccctttttca cgacctcgac cttttcgatc gccggaagat gatagggata
     5221 gatcttttcg acgccgatcc cgttcgcgcc gatctttcgt accgtgaagg ttttattgag
     5281 accgtccccc cgtttttgta tgacgatccc ttcgaatttt tgggtccgtt ctttattccc
     5341 ttccttgatc ctttgaaaaa tccggaccgt atcccccacg ttaaatgggg gcaattctct
     5401 gtagtcctgt tgcgccatcg ctcgaataaa cgcgtttgac atacctcttc ctcctttatt
     5461 tttttaacat tcttttttgg gatgtctttg aatcaatagt tttctcgtat caaccgatcc
     5521 aagatgatgg atacggcgct gcgaacggac aggtggttaa aggatcctcc cgggcgcacc
     5581 ggctcgagat cgtagtcgca aagcgacaac acttcggcgg gcagccccca tccggtaccg
     5641 aagagtaaaa cgatcggtcg ggtttccgtc agaagcagtc tctggccctc gtcgtaggtc
     5701 atcgaactgg gccgcttttt cgccgacgtg aagattagca ccggtttttg gttctctatt
     5761 ctctcgatct cctcgagcgc gtcctccaga tacggcttca ggatcacgta gttaagcgct
     5821 tctgagcgat tggggttgta gcggattccg gacccttcgg tccaaaactg aaccaccctt
     5881 cgcacgatct cctgttgcgc cgggagatta gtcaccatat agtacttttt cacgttgtag
     5941 gtccggcata cacgcgctat atcgtgtata tccacattgg taacggcggt cgcgatgatc
     6001 tgttgatcac gtccgagaca aggataatgc accaatagaa catatatttt atccagcatc
     6061 agtggagagc tcccggatga tttctataat tgcgttcttc tcctccggac cgatctcttt
     6121 ttccaagaac agtttcgggt gcttgagtat cgtccgaaga atagaatcgc gtttgcgggc
     6181 tttttcgatc tcttggtgat ggccgttaac gtacactgcg ggtacctcaa gtccccgata
     6241 cacggccggc ctcgtccaat tcgcgtgatc cagtagcccg ttgtaaaaag aatcctgttt
     6301 caccgaccgc tcgttgccta ccactccggg cacgaaacgg ctgatcgcat caatcatcaa
     6361 caaggcgggg atctctccgc cgcttacgac gaagctgccg atagagagtt cgtagtccac
     6421 gagggtcata atccgttcgt cgatgccttc gtaccttcca cacagaaaga ggaggttctc
     6481 ctgtcgagcc agctcctcag ctctagcgga gtcgaaaatc tggccttgcg gtgaaggaaa
     6541 gacgacctta aacgggccgt tccgcgtggc gtagtcgtct atacactcaa agacgggcgc
     6601 gggtttcatc accattccac cgcccccgcc gtagacgtag tcgtcggttg tcctgtgggc
     6661 gtcgtgggta tagtccctga gctgtatgac ctcgatctct agggtcttgt tcgcgcgagc
     6721 cctactgact atgcccctgg aaagaaaggc ttcgatcatc tcgggaaaaa tcgaaacgac
     6781 agcgaatttc atcggcgtga ctagaatcct tcatcggagc cggcgtccgt ttcatcgtct
     6841 ttcacggcca tatagaccgg agggcgaatc accaaccgct tctgttccaa atccatcgaa
     6901 acgagaaaat cttttgccat cgggaccaac agctcggaat ccttcggggg atctcccggc
     6961 cccgaaaatg gtttccgtat agacagaacg tccgcgctcc ccgtttcgat cacatcctcc
     7021 acgactccca accgttggcc ctgctcgtcg taaacggagg cgccgagcag ctgaaagaaa
     7081 tagtactccc ccgctttgag caggggtaga tcgcagaggt cgaggtatac ctcataccct
     7141 tccaattctt tggccaaatc gatgtggtcg tagttcctaa acctcaagat catccgatcg
     7201 ccacggcgat ggaaggcgtc gagggtacaa cgcagatgac ttcgcttctt cgagtgaaag
     7261 aggaaaaact ccttccccgt ttcgaagact cccgggtagt tcgcgagcat cgtggctttg
     7321 atctctccgc gtaacccgtg agggcctaaa agacgcgcga tcggcacgga atgttccaga
     7381 attttctcaa tttgatggat cacggaaagc tcaccgctgc actctcaaga taaacttttc
     7441 ttctttttga ccagccaacg cgttcagaag gatattgatc gacttgatcg ttcgcccgtc
     7501 tttaccgatc acctggccca cgtcttcgac gtgaagcctc aggtcgtaca gctcgctcgg
     7561 cggagaatcc ggggttttct cgtcgctgcg agaaactcgg acgtcctccg ggtgtttcac
     7621 gattcctttg atgatgaact ctagcgcatc cttcatcatg acaagccgat tatttctgct
     7681 caggcgtttc cggcgagggg tggctcgacg cctctcgctt gcgcttttct cgatcgaagc
     7741 tttccatcac gccatgtttg gacaagatgt ctcggaccgt atccgtgggc tgggcgcctt
     7801 tcagaagcca agcgagcgct ttttcggtat ccaatttcga caagttttgg tcattgatgg
     7861 ggtcatagaa cccgatcgac tcgatgtatc tgccatctcg ccgcttgttc gcttcaatga
     7921 ccactacgcg ataaaaaggc cttttgcgct ttcctaaccg tgttaaacgt atttttacca
     7981 tgaaacccat aacctcctca tatattaaac acttagtatt cagaactaga acggaagacg
     8041 gctcaaccct ttaggcccca ttttcccgat ttttttcacc atcttcttca tgttttcgaa
     8101 gttatcgagc aatttcgtga cctctggaag cggtctccca ctacctaagg cgatcctttt
     8161 ctttcggcta tagttgatca gctttgggtt cgcgcgctcc tttttcgtca tcgaacagat
     8221 gatcgcttcc acgtgtttaa tctgcttctc gtcaacggtg acacccttca gcttgctccc
     8281 accgggaagc atttccaaga tcgaccgtaa ggaacccatt ttttgtatct ggcggatttg
     8341 ctccaagaaa tcgttaaagg tgaagtccgc tttcctgagt ttcttttcga gctctttggc
     8401 tttcttttcg tctatcgttt gctccacctt ttcgatgagt gagagcacgt cgcctaaccc
     8461 gagtatgcgc gaggaaagac ggtcggggta aaaagcctcc aacccgtcaa tcttctcgct
     8521 gaccccgatg aacttgatcg gctttccggt gacgcttcga atcgataggg caacaccgcc
     8581 gcgggcgtct ccgtccatct tcgtcaagac gaacccggtc accgctaagc ggtcattgaa
     8641 tgttttcgcc gtttgaaccg catcctgccc gatcatcgcg tccaaaacca tcaagacctc
     8701 gtcgggcttg acgatctccc gtaccttctc cacttcgcgc atcatctctt cattgatgtg
     8761 aagccgaccg gcggtgtcga agatcaccac gtcatatagg gacccgatcg ccaacttcat
     8821 cccctcttga cagatcttgt cgacgtggcg ccgatccccg gaaaagacgg tgacgcctat
     8881 ctccgcgcct agtatttcga gttgatcgat cgcggcaggg cgatagatgt ccgcggcgac
     8941 caacagcact ttccgccctt cgctcttgat gatgcgcgca agcttcgccc cggtcgtggt
     9001 tttcccgcta ccctgtagcc caacgagcat aatcggcgaa ggtttctggg tgaaacggag
     9061 cttctcgtgc ttttctccca tcaacgccgt cagctcgtct cggacaattt tgatgaattg
     9121 ttggtccggc gtcagactgt tgaaaacctc cgcgcctatg gccttctctt tcaccttttc
     9181 gatgaagtct ttgaccactt tgtagttgac atcggcttcc agcaacgaca atttgaccat
     9241 ccgtatcgct tcctggatgt tcttttccga tatcttcccc tgtcctcgta aggatttaaa
     9301 cgctttttcg agtttttctt gcaggctttc gaacaaccaa atccctccta gaaaaaacct
     9361 ctcgagtata cagtaaaagg gtatgagagt caatcgaaat gacgctttcc cacgaaaaat
     9421 ctggcataaa tatgcgttaa ttatagttta cgtcgtatgc ttgactctgt gaatccattg
     9481 gctaaatgcg tgggtatagt atcgaggtag aacaaaaaga cggaaagcgg aggaggagaa
     9541 gaaaaacggc tctagtttga gccgccatta gagctcattt gacccttcaa ggggtgggta
     9601 aagaagtgta tttttcgacc ccgcgaagag tacgaagatt atcgcggggt aaccagcagg
     9661 ctggcttctt gtgtgttatc gcggggtaag tggttttcaa gaccccgcga cccgcccctt
     9721 cttaaaagcg tatcgcgccg tcgctaaaag aaggagcgct tacgccgcga tgacctcacg
     9781 gactaaaaat cgcgcggccg attccggcct tgtttcttga gcgcactcca taacttctca
     9841 cacacgaccc atcaaatcgc gttcaagcct tttgtcagac tatctacat
//
LOCUS       DAOPXN010000073         9817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_6961_length_9817_cov_5.003688, whole genome shotgun sequence.
ACCESSION   DAOPXN010000073 DAOPXN010000000
VERSION     DAOPXN010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9817
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6961_length_9817_cov_5.003688"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(337..3807)
                     /locus_tag="PKM49_05540"
     CDS             complement(337..3807)
                     /locus_tag="PKM49_05540"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNY82311.1"
                     /translation="MAKIKFAETWWGESWIRALEEIDVDTNRLPRGRTYARNGSVLEI
                     KRTRTGTIQARVQGRRPAPYRIRIALTEWSEEQNAAVLDLIQNSPALSSQLLIGQMPE
                     SLEAELEKLGARIFPSSWREMTASCSCPDWANPCKHLAAVYYLLAVEIDKDPFLLFEM
                     RGLKKKRILETARLTDQTLKSAGWVFDVSQTPVTDTYEAPTPTTEEIESYIVQPYPID
                     KALGVLEKNPRFDDGPLILQLLPTTYRKIRDYWNGEAFIEPEEPLAHSSSITIEHREE
                     GPVFLVDELYKKEVSELTVADFLFRMEGVSLSPLAEDHATMRWVKDTLSLARRLIALN
                     SWVPVLYSVGQGEDFGIRYAPVYSQPTLRQKKVALNASVPTGLIHDAKNRPLKREQSV
                     DYLLSVFLTQMVQEPLDGVLPYSSIEMARVFFLGEVYKADHFQRQQTKTAVQNWLTPL
                     HLSDSEHNLVLDIQVTKKTQFSLTVSVKRKRDLFIDAVPMQNFLKTAANGGEKEGALK
                     QLACLSEWIPPLKRSFEERTIPITVDELIQLLSQARDFLGFFGAELVVPKEFKDLTRP
                     RVFASARMKSERTKGVLNFENLLQFEWRVQFGEDNLSIEEFAALMKGAERIVLFKDQY
                     CLVDPIEAKRMVEKYMTSPIISGPEGLRAVFAQELDGYPLRYGEDVERLLKGLRKERR
                     CEAPGTLNGQLRPYQLKGYQWMLSNLHNGFNICLADDMGLGKTIQVIAVLLKLRENKE
                     LTRPALVICPASVTRNWRKEIETFAPDLKCELYGGSNREISAGKDVWITTYGMVRSEV
                     ERLKSFEWSVVIVDEAQNIKNPDADQSKAIAQLNGQYKIAMSGTPVENRVAELWSLFN
                     FIMPGYLGPRKKFREEFAIPIERLNDPVKTKTLKQAVEPFLLRRLKSDKNIIKDLPEK
                     VVREVYVTLKPEQAALYGTVLEAAVGEIESAQGIKRKGKVFWLLTALKQICNHPVSYT
                     KSGTPVAEHSGKSEKAFELLKEVIKSGEKALLFTQYREMGALLAAMTPEKLGIDPLFF
                     HGGLTPKERENLILDFQQQPYHPLMIITLKAGGTGLNLTAATTVIHYDLWWNPAVENQ
                     ATDRSYRIGQKKSVTVHRLLTENTFEERVNELLQKKKELSDAIVGAGEHWITEMSNEE
                     IRSLFEMKTT"
     gene            4501..5247
                     /locus_tag="PKM49_05545"
     CDS             4501..5247
                     /locus_tag="PKM49_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNY82312.1"
                     /translation="MSFLIFGHRGYSSEYPENTTLAFRKAMEAGADGVELDIRASRDG
                     KVVVIHDEDTLRVAGIPGKVAELDYADIRKAKVQNGLSIPLLDEVFESFPDDGFLNVE
                     IKSPDAVAPLVKLMKTYEPSFQIVFSSFLFEPLEELRNALPEVKIGLLIGEGLVKYVS
                     VSHTSVNTTSFEALLAGYLPYSLHLPMQLFNRLPVEGTVRFLQKVKASNIRLFWWTID
                     DIPVTEFLRDRSIIDGVITNQVGRMVRAYK"
     gene            5265..6170
                     /locus_tag="PKM49_05550"
     CDS             5265..6170
                     /locus_tag="PKM49_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82313.1"
                     /translation="MAKQKSERRCPAGTVALGVDETVETAYQIVGGRVQIEWKWDTWE
                     NFYLQSPFVFGALECIERAKSTIRVQANEDTVLRPLSAEELSNPEDPELAASVLQSLT
                     LFLKQIVQEKYFGLALSESEKMYRAFETYVKKADKQRAIDCYSRFVTGFPTSPFIDRM
                     LHYIQDISLGKDLVVEIPENDEDAFLSILTQATDADPLQNLSLLKKFEERFPNSAHYE
                     RIIDMIIGEYDKLGDEYQLNHYLRKFIYTYPSSSTADQKLLMLISVQRKSGEPSWYEN
                     GLRFLLLYPESELIGTVRKFMGIDR"
     gene            6171..6482
                     /locus_tag="PKM49_05555"
     CDS             6171..6482
                     /locus_tag="PKM49_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82314.1"
                     /translation="MRGLTKNQRLNYIVLIPFYIWVVFFLFSLALFPFFPRFSADFLF
                     AVGILTVNLSWILLIRRRKDEDQEDADKGKRKDIAGYGFLVFTGLAFLSSWVFSLFYR
                     G"
     gene            6629..7438
                     /locus_tag="PKM49_05560"
     CDS             6629..7438
                     /locus_tag="PKM49_05560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNY82315.1"
                     /translation="MRFRRFILWVFCLCLCSSISFSYVALRHFVNSGDTIETICRIYQ
                     LPVSVFLDWNRSGSPLKLSPGMVVTIPFPSGYVYRVAEGNSLSWISCSFFADLNAIAA
                     ANGLKFPYTLRIGQEIFIPYGEIGRKFYSEPGKLLWPVYGEISSLYGNRVHPITGLKA
                     FHAGVDIAAPEGSPIFASASGTVSHAGENGGYGIMIEIADATQKYRYGHLSALCVTVG
                     QNVKRGEVIGRVGSTGDSTGPHLHFEIRNQKNETLDPLSQLPNIKNIYPKH"
     gene            7454..8647
                     /locus_tag="PKM49_05565"
     CDS             7454..8647
                     /locus_tag="PKM49_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009369326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HNY82316.1"
                     /translation="MIDFRKIQDLSKRYEMDMVRFLRDMIRIPSPSSQEKRVVDRVAE
                     EMKKVGFDEVQIDPFGNVIGRIGYGKRIIAYDAHVDTVGIGNPDTWDFDPYEGKYENG
                     IVYGRGASDQEGGMAAMVYAGKMMKELGLNKDCSCYFVGSIMEEDCDGLCWQYILREG
                     ILRPEVVIITEPTNLNIYRGHRGRMEIQITVDGRSCHASAPERGDNAIYKMMPIVAEI
                     EKLNERLRHDDFLGKGTIAVTEISSVSPSFNAIADRCTISLDRRLTAGETKASSVEEL
                     QRVLDTQGVAGKIEVLQYKTPTYRGVEYPVEKYFPTWVFPEEHPIIQQAAATYRSLFN
                     REPLIDKWTFSTNGIATAGIFSIPTFGFGPANEIYAHSNQDQCPSEHLVKAAAFYCAF
                     PSHYR"
     gene            8682..>9817
                     /gene="murJ"
                     /locus_tag="PKM49_05570"
     CDS             8682..>9817
                     /gene="murJ"
                     /locus_tag="PKM49_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNY82317.1"
                     /translation="MSKTIKFSLYMAVATLMSRVLGLVRDAMFANVFGSSPEYDAYLV
                     AVLLPFFLRRIFGEGALQSAFVPIYNKRAMIDSRSATRFANTIFTVFVPILILCTLLG
                     YYFMPYLIYLFAPGMEPAIREMAVMCGVVAFPFIIFISLWAIKTGILNSHDIYFVPAF
                     SPAIHNVFTIAGILLSPYFTPRILGPTVFFLIGGAFQFISVLFIGKKKTGYRFEIDFV
                     REDMKEFSSLFLSSMAAVSITQINSLIDTIVVSHLGQGSISLLQYANRLYQLPLGVIG
                     VSVSTVALSQLSKRSLDSFREMVTDNLEKLLLLILPAAAGMILLREGLVVFLFQRGAF
                     GIYETVFTSKILLGYLFGLPFYCVYFLLSKAEYALGKGNVAFFA"
BASE COUNT         2198 a         2506 c         2576 g         2537 t
ORIGIN      
        1 ctatgaagct gtccttttcc gcctgtatcg tgccgacatc gatagtctgt aagacatcag
       61 ggggaagata ggtctctaag aacgcttggg tatttgcgac atcccccagc acttctttga
      121 aaaacctatc gtgggggtta ttcagtcttt ccatctgtgg cctccctacg ggaaatcatt
      181 tcacgggtat tatacacgat gcaactcgag gatgggtata atagtgtggg tataatatcg
      241 gggaacgcca aaatgggaac gccgaacttt agtccggcgt tcccagcaat acagcgcccc
      301 ggcggcgatc tcagtacgga gaggatagct cgcgggttag gttgttttca tttcaaaaag
      361 gcttcggatt tcctcgttgc tcatctccgt gatccaatgc tcgccagcgc cgacgatggc
      421 gtcggagagc tctttctttt tttgtaagag ctcgttcacg cgttcttcaa aggtgttctc
      481 ggtcagtaac cgatggacgg tgacgctttt tttttgaccg attcggtagc tcctgtccgt
      541 cgcctggttt tcgacggccg ggttccacca taggtcgtaa tggataacgg tggtcgcggc
      601 ggtcaggttt aagcccgtgc cacccgcctt cagtgtaatg atcattaatg gatgataggg
      661 ttgctgctga aagtcgagga tgaggttttc tcgctcttta ggggtgagcc cgccgtggaa
      721 aaagagcggg tcgattccta gcttttcggg tgtcatcgca gccaataagg cgcccatctc
      781 gcggtattgg gtgaagagta acgccttttc accgcttttg atgacttctt tcagcaattc
      841 aaaggctttc tcgctctttc cggagtgctc ggcgacggga gtcccgctct tggtatagga
      901 cacggggtgg ttgcagattt gtttgagcgc ggtgagaagc caaaatacct ttcctttccg
      961 cttgatgcct tgggcgcttt ctatctcgcc taccgcggct tctaagacgg tgccgtacaa
     1021 agcagcttgt tcgggtttga gcgtgacgta gacctcccga acgacttttt ccggaaggtc
     1081 tttgatgatg ttcttgtctg attttaacct tctcagtaaa aacggttcga ctgcctgttt
     1141 cagcgtcttg gtcttaaccg ggtcgttcag gcgttcgatg gggatcgcga attcttctcg
     1201 gaattttttc ctgggaccta agtatcccgg cataatgaag ttaaacagac tccatagttc
     1261 cgccacgcgg ttttctactg gagtcccgct catcgcgatc ttatattgcc cgttgagctg
     1321 ggcgatagct ttagattggt cggcatcggg gttcttgata ttctgcgctt catcgacgat
     1381 tacgacgctc cactcaaaac tcttcaagcg ttctacctcg gatcggacca taccgtacgt
     1441 cgttatccaa acgtctttcc ctgctgatat ctcgcgattc gacccgccat acagctcgca
     1501 cttcaggtcc ggcgcgaagg tctctatctc ttttcgccag ttacgcgtta cggaagcagg
     1561 gcaaatgacc aaggcggggc gcgtcaactc tttattttcg cgcagtttga gcaacaccgc
     1621 gatcacctgt atcgttttac caagacccat atcgtccgcc aagcatatgt tgaacccgtt
     1681 gtgcaggttc gacaacatcc attggtaccc tttcaactga tacggtcgca attggccatt
     1741 gagggtaccc ggtgcttcgc accgccgctc ttttcgcaaa cccttcaaca gccgttcgac
     1801 gtcctctccg tagcgtagcg ggtatccgtc gagctcctgg gcgaatacgg cgcgcagccc
     1861 ctccggccca ctgatgatgg gcgaagtcat atatttctca accatcctct tcgcttcgat
     1921 agggtctacc aagcaatact ggtctttgaa aagtacgatc cgctccgcac ccttcatcaa
     1981 tgccgcaaac tcctctatag acaggttgtc ttctccgaac tggacccgcc attcgaattg
     2041 gaggaggttt tcgaagttta aaacgccttt cgttcgttcg cttttcattc ttgcgctcgc
     2101 gaagacccgt ggacgcgtta agtccttaaa ctctttcggc acgacgagtt ccgctccgaa
     2161 aaaaccgagg aagtctcgcg cttggctgag taactggatg agctcatcca cggtgatagg
     2221 gatcgttcgt tcttcgaaag agcgctttag gggaggaatc cattcggaaa ggcaggcgag
     2281 ctgcttgagc gccccttctt tttcgccccc gttcgcggcg gttttcagga agttttgcat
     2341 cggtacggcg tcgataaaca ggtcgcgctt gcgcttcacg gagaccgtca acgaaaactg
     2401 ggtttttttc gttacctgga tgtccaggac gagattgtgc tccgagtcgg aaaggtgtaa
     2461 cggggtcagc cagttttgca cagccgtttt cgtctgttgc cgttgaaaat gatccgcctt
     2521 atacacctcc ccaaggaaga agacgcgcgc catttctatc gacgagtaag gcagcacacc
     2581 gtctaaaggc tcttgcacca tctgtgtcaa aaagacggac aagagatagt ccacgctctg
     2641 ttctctcttc agagggcggt ttttggcgtc gtgaataagt ccggtgggaa ccgaagcgtt
     2701 caacgccact ttcttctggc gaagagtcgg ttgggagtag acgggggcgt atcggatccc
     2761 gaagtcctcg ccttgtccta ccgagtaaag aacgggaacc cagctgttta acgcgatgag
     2821 tcttcgggcg agggagagag tgtctttcac ccaacgcatc gtagcgtgat cttctgcaag
     2881 cggcgagagg ctgacccctt ccatccgaaa caaaaaatct gcgactgtga gctcagaaac
     2941 ctcttttttg taaagctcat ctaccaaaaa cacgggtccc tcttcgcgat gctctatcgt
     3001 tatcgaagac gaatgagcaa ggggctcttc gggttcgatg aacgcttctc cgttccaata
     3061 atctctaatc ttccggtaag tcgtgggcag aagctgcagg atgagggggc catcgtcaaa
     3121 ccggggattt ttttctaaaa ctcccaacgc cttatcgatg ggatagggtt gcacgatata
     3181 gctttcgatt tcctccgtcg tgggggtcgg agcttcgtat gtatcggtca caggagtttg
     3241 agatacgtcg aaaacccacc ccgcgctttt caaggtctga tcggtaagcc gcgcggtttc
     3301 taagatccgt ttctttttca gcccacgcat ttcgaagagc aagaaggggt ctttgtctat
     3361 ttcgacggcc agcaggtaat agacggcggc gaggtgctta cagggattcg cccagtcggg
     3421 gcaactgcaa gaggccgtca tttcgcgcca agaagaggga aagatccggg caccaagctt
     3481 ctccaactcc gcctcgagac tttcgggcat ctgaccgatc aaaagctggg aggagagcgc
     3541 ggggctgttt tgtattagat cgaggacggc ggcgttttgc tcttccgacc attccgttaa
     3601 agcgatacgt atacgatagg gggcgggtcg acgcccctgc accctcgctt ggatcgtccc
     3661 cgttctcgtt cgtttaattt ctagaaccga cccgtttctc gcataggtgc ggccccttgg
     3721 cagccgattc gtatccacgt cgatttcttc gagggccctg atccagctct ctccccacca
     3781 agtctccgcg aacttgattt ttgccacaag taaccacctc ggttttgatc gtttgtcatt
     3841 ttacaagcgt ctaacggctg cggtttttca cctagtgaat tatacacgaa acggtgacag
     3901 gtgcggggag ttcgtgtggg tataacatcg aaatgcgaca aaataagata tgaggaggag
     3961 gtccgctttt tatccttttt acgctgaggc tcttgtgagg ttatcgcggg gtaagtggtt
     4021 tttacgaccc cgcgatccgg ccttagcctt ctttagccgc gataggctcg gaaaagaggt
     4081 atcgcgccgt cgctaaaaga agggacgctt acgccgcgat gcctgtgtga agttatcgcg
     4141 gggtaaccaa cgggacccca cgatactttc atacctttgc gatacgcgct gctgcatttc
     4201 tattttcaca aaccatgcca aactaaagtt tggcgttcgc aaggcgttcc caagattctt
     4261 ttctctgtgt tctctgtgcc ctctgtgcga accctgtttt tcaagacagg cacaaaagcg
     4321 tatcgcgccg tcgcgaaaag aaggggcgct tacgccgcga tgactatatg ggctaaaaac
     4381 agtgtgatcg attttggcct tgtttcttga gtacacccca taacttgaca cacaccctga
     4441 tgactgaata aaccgcggat agtgtataat agactgtcaa aacgaaacga ggtggtttct
     4501 gtgtcttttc tcatctttgg tcaccgtggc tactcgagcg agtaccctga aaacaccact
     4561 ttggcatttc gaaaggcgat ggaagccggg gcagatggcg tggaactcga catccgagcg
     4621 tcgagagacg gcaaggtcgt cgtgatccat gacgaggata ccttacgcgt ggcgggtatc
     4681 cctggcaagg tcgccgaact cgactacgcg gatatccgca aagcaaaagt ccagaacggg
     4741 ttgtctattc cgttgttgga tgaagtcttt gaatcctttc ccgacgacgg gttcctgaac
     4801 gtggagatca agtcaccgga tgccgttgcc ccgctcgtca aactgatgaa gacgtacgaa
     4861 ccgtcttttc agatcgtgtt ttcgtccttc ttatttgagc cgttggaaga acttcgaaac
     4921 gcgctacccg aagtcaaaat cggtctattg atcggcgaag gactggtgaa atacgtgtcc
     4981 gtgtcgcata cttcggtcaa caccacctcc ttcgaagcac ttttggccgg atacctcccc
     5041 tactcgctgc acctgcctat gcaactcttc aaccgtttgc ctgtagaggg gaccgttcga
     5101 ttcttgcaaa aggtcaaggc ttccaacatc cgactctttt ggtggacgat cgacgatatc
     5161 ccggtcaccg agtttttaag agaccgcagc atcatagacg gcgtgatcac caaccaggta
     5221 ggaaggatgg tccgcgccta taaataggcg gtagctttcg gaagatggct aaacagaaaa
     5281 gtgaacgacg gtgccccgcg gggacggtag cccttggagt cgacgaaacc gtggaaaccg
     5341 cttaccaaat cgtcggcggt cgtgtacaga tagaatggaa atgggacact tgggaaaact
     5401 tctacctaca aagccccttc gtctttggtg cgctagaatg cattgaaagg gcgaagtcga
     5461 ccatacgggt gcaggcgaat gaagataccg tgttgagacc cctgtccgcc gaagagctct
     5521 cgaatccgga ggatccagag ctagccgcct ctgtcctcca atccttaacc ctttttttaa
     5581 agcaaatcgt acaagaaaag tattttgggt tggcgctatc cgaatccgag aagatgtacc
     5641 gcgcctttga aacctatgtg aagaaggcgg acaaacaacg cgccatagac tgttactctc
     5701 gtttcgtcac cggctttccg acgtcgccgt ttatagaccg aatgctccac tatatccagg
     5761 atatttcttt gggcaaagac ctggtagtgg agatacccga aaacgatgag gacgcctttc
     5821 tgtcgatctt aacacaggcc accgacgccg acccactaca aaacctctcg cttctgaaga
     5881 aattcgagga gaggtttccg aactcggccc attacgaacg aattatcgat atgatcatcg
     5941 gagagtatga taaactgggc gacgagtatc aattgaacca ttacctccgt aagttcatct
     6001 acacctatcc gagttcttca acggcggatc aaaagctgtt gatgctaatc tcggttcaga
     6061 ggaaaagcgg agagccttcc tggtacgaaa acgggttgcg gtttttgctt ttatatcccg
     6121 agagcgagct catcggcacc gtgagaaagt ttatggggat cgaccgatag atgagaggat
     6181 taacaaaaaa ccaaaggtta aattatatcg ttctcattcc gttttacatc tgggtagttt
     6241 ttttcctttt ttctctggcg ctgtttcctt tttttccccg tttttccgcg gattttctat
     6301 tcgccgtcgg gatcctcacc gtgaaccttt cttggatcct gttgatacga aggcggaagg
     6361 atgaggacca agaagatgcg gacaaaggga agcggaagga tatcgctggt tatgggtttt
     6421 tggtctttac gggactcgcc ttcctcagct cttgggtgtt cagtcttttc tatcgcggct
     6481 agttcgggtc caaagataga gccgccgcag gactcaaccc ggagaagggc gcgtcgagtc
     6541 ctcgtgcgcg tttcgacggg gtttttcgtg atgtgcccat tcggaaaggc gtttgtggtc
     6601 ccgatattgt gatacttggg gggttttgat gcgttttcga aggttcattc tctgggtttt
     6661 ctgcctttgt ctttgttcct cgatatcttt ctcttacgtg gcgctccgcc attttgtcaa
     6721 ttcgggcgat acgatagaga ccatctgtcg gatctatcag ttgcctgtgt cggtctttct
     6781 cgattggaat cgctcagggt cccctctcaa gctgtctccc ggtatggttg tcaccatccc
     6841 cttcccgagc ggctacgtct atcgcgtcgc ggaaggaaat agcctgagct ggatctcctg
     6901 ctcgtttttt gccgacctga acgctatcgc cgcggccaac ggcctgaagt tcccgtacac
     6961 cctacgtatt ggacaggaga tattcatccc ttacggtgag attgggagaa agttctattc
     7021 ggaacccggc aagttgttgt ggccagttta tggagagatc agctctttat acggaaaccg
     7081 agtccatccg atcaccggtt tgaaagcgtt ccacgccggg gtggacatcg ccgccccgga
     7141 agggtcgccg atcttcgcga gcgcgtcggg gaccgtgagc cacgcgggag aaaacggcgg
     7201 ttacggcatt atgatcgaaa tagcggacgc gacacagaag tatcggtacg gacacctttc
     7261 tgccctgtgc gtcaccgttg gccaaaatgt gaaaaggggg gaagtgattg gccgggtcgg
     7321 aagcaccggt gacagtaccg gcccgcactt gcacttcgaa atccgaaacc aaaaaaatga
     7381 aacactggat ccactatctc agttgcctaa catcaaaaat atttatccga aacattaaag
     7441 aaggagtgat acgatgatcg attttcgaaa aatccaagat ctctcaaaac gttacgaaat
     7501 ggatatggtc cgttttttgc gagacatgat tcgaatcccc agcccctcat cacaagaaaa
     7561 gcgtgtggtg gaccgcgtcg ccgaagaaat gaaaaaagtg gggtttgatg aggtccaaat
     7621 cgaccccttc ggaaacgtga tcggcagaat tggctatgga aaaaggataa tcgcctacga
     7681 cgcgcacgtc gacacggttg gcataggaaa cccggatact tgggattttg atccctatga
     7741 aggcaagtac gaaaatggga ttgtatacgg gagaggggcc agtgatcaag agggcgggat
     7801 ggctgcgatg gtctacgccg gaaagatgat gaaagagctc ggcttgaaca aagactgttc
     7861 ctgctatttc gtcggatcga ttatggaaga agactgcgac gggctatgct ggcagtatat
     7921 cctgagagaa ggaatcttgc ggccagaagt ggtgatcatc accgaaccga cgaacctcaa
     7981 catctatcgt ggccaccggg ggcgaatgga gattcagatc accgtagacg gaagatcgtg
     8041 ccatgcgagc gccccggaac ggggagacaa cgcgatctac aagatgatgc cgatcgtagc
     8101 cgagatcgaa aagctgaacg agcgcttacg tcacgatgac tttttgggaa aaggaacgat
     8161 cgcggttacc gagatttcct ccgtctctcc ttctttcaac gcgatcgccg atcgctgcac
     8221 gataagcctc gaccgcaggt tgactgcagg agaaacgaaa gcgagtagtg tcgaggaact
     8281 ccagcgcgtt ctggacacac agggtgttgc gggcaaaatc gaggtgttac agtataaaac
     8341 gcctacctat aggggagtgg aataccccgt agaaaagtat ttcccaacgt gggttttccc
     8401 tgaagaacac cccatcattc agcaggccgc ggcgacctat cggagcctat tcaatcgaga
     8461 gcctttgata gacaaatgga ccttcagtac gaacgggata gcgaccgccg ggatcttttc
     8521 tatcccgacc ttcggatttg gtccagcgaa cgaaatctac gcccatagta accaagacca
     8581 gtgcccttct gagcacttgg tcaaagccgc ggcgttttat tgcgcctttc catcacacta
     8641 tcgttgagtg tatgaaagcc agagggtaag cgaaccgcat cgtgtcgaaa acgatcaagt
     8701 tttctctcta catggccgtt gccaccttga tgagccgcgt gttgggactt gtccgggacg
     8761 cgatgttcgc caacgtattc gggagttctc ccgaatatga cgcctatctc gtagcggttt
     8821 tacttccttt tttcttacga cgtattttcg gtgaaggcgc tctgcagagc gccttcgttc
     8881 cgatctacaa caagcgggct atgatcgatt cgcgttccgc cacacgattc gccaacacca
     8941 tttttacggt gttcgtgcca atcctgatcc tttgcaccct cttaggttac tattttatgc
     9001 cttatttgat ctacttgttc gcgccgggta tggaaccggc aatacgagaa atggccgtga
     9061 tgtgtggggt tgtcgccttc cctttcatca tctttatcag cctatgggcg atcaaaacgg
     9121 gaattctgaa tagccacgac atctatttcg ttccggcctt ctcgcccgcg atccacaacg
     9181 tgttcacgat cgccggtatc ttgctctctc cctacttcac ccctcgtatc cttgggccga
     9241 cggtcttttt tttgatcggc ggagctttcc aattcatcag cgttttgttt atcggaaaga
     9301 aaaagacggg gtaccgtttc gagatcgatt tcgtgagaga ggatatgaaa gagttctcga
     9361 gcctattttt aagctcgatg gccgccgttt cgatcacgca gatcaattct ctgatcgata
     9421 cgatcgtcgt ttcgcacctg ggtcaaggga gcatctcttt gctacagtac gccaatcgtt
     9481 tgtatcagct gcctttgggg gtcatcggcg tgtcggtgtc gaccgttgcc ttatcccagc
     9541 tctccaaacg ctctttggat tctttccgag aaatggtcac cgacaacttg gaaaagctgt
     9601 tgttgctcat cctgccggcg gctgcgggga tgatcctgtt gagggaagga ttggtcgtct
     9661 tcctctttca gagaggggca ttcgggattt atgagacggt cttcacttcg aagatcctac
     9721 tcgggtatct cttcggactt ccgttctatt gcgtctattt cctgctttcc aaagcggagt
     9781 acgcgctggg aaaagggaat gtcgcgtttt tcgcgag
//
LOCUS       DAOPXN010000074         9782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7000_length_9782_cov_5.635139, whole genome shotgun sequence.
ACCESSION   DAOPXN010000074 DAOPXN010000000
VERSION     DAOPXN010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_9782_cov_5.635139"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..2149)
                     /gene="tpiA"
                     /locus_tag="PKM49_05575"
     CDS             complement(185..2149)
                     /gene="tpiA"
                     /locus_tag="PKM49_05575"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNY82318.1"
                     /translation="MEALTIRDVELTGKNVLMRVDFNVPLDKKTNQVTDDTRIRAALP
                     TIRYALEKGARVILLSHLGRPKGGPDPKYSLLPVVSALSALLEKPVLFSSVFVGEESE
                     QKVRKLQNGEVLLLENTRFDPREEKNDKGLSRELATYGDLLINDAFGTAHRAHASNVG
                     VADYLPSYSGFLMEKELRFLGKLVEKPEHPYVAVLGGAKVSDKIGVIENLLTKADRLL
                     IGGAMMFTFWKALGFETGSSLVEEDKIPLAKALLEKSGERSVEILLPIDVVVSEKMES
                     GQIKRTVTREAGVPRGWMGLDIGERTIALFKEAMKGAKTIVWNGPMGVFELPEFEEGT
                     RAIAVALSERKDATTVVGGGDSAAAIAKFGLEEEVTHVSTGGGASLEMLEGKEMPGVA
                     SLCVRKSRKMVVAGNWKMYKTPDEAGAFARRLLQVLPKSFEPVEVVLCPPYVDLHAVR
                     DVLVGTPLAIGAQNVYPAEEGAFTGEISPRMLVASGVRYVIVGHSERRAIFKETNDFI
                     NTKLKSLLASGLKPIFCVGETLSQRESGETLSVLDNQVTEGLKGLPAPDVEKIAIAYE
                     PVWAIGTGKVALPWQAQEACSRIREKLSDLYGAEVASKVSILYGGSIKPENFASLMAR
                     PDVDGGLVGGASLQESFADLVKTALTFRPI"
     gene            complement(2203..3210)
                     /gene="gap"
                     /locus_tag="PKM49_05580"
     CDS             complement(2203..3210)
                     /gene="gap"
                     /locus_tag="PKM49_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNY82319.1"
                     /translation="MPKVPIAINGFGRIGRLVLREIVKRGGYEVVLINDLTDAKTLAY
                     LFKYDSVHRTFGGTVEHGEDFIKVNGKSIKVTAQKNPADLPLKALGAEMVIESTGVFT
                     GKDKCQAHLDAGAKKVLITAPEKGGVDATIIIGCNEGILTPEMKVVSNASCTTNSIAP
                     IVKVLHETFGVQKGLLTTVHAYTNDQRILDLPHKDLRRARAAALNTIPTTTGAAKAIG
                     LVIPQLKGKLDGIALRVPVSDGSITDMTIVLEKPTTKEEVNRVMKEACSGKLKGIIDY
                     NEEMIVSSDIIGLTSSGVFDATLTAVMDGNLLKICSWYDNEYGYSCRVVDLVGLMMKG
                     M"
     gene            complement(3234..3602)
                     /locus_tag="PKM49_05585"
     CDS             complement(3234..3602)
                     /locus_tag="PKM49_05585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNY82320.1"
                     /translation="MAKKMRSFGGKSLRPSSTSGKPSMQDLMTKINQAGETMQEIEES
                     FAQKSFEITAGGGAIKITMKGDFHISAIELEDGLLEEKEELTDLLSAAFNQAIETVSK
                     EKDAELEQLKTQLGIGGLGI"
     gene            complement(3618..5021)
                     /gene="dnaX"
                     /locus_tag="PKM49_05590"
     CDS             complement(3618..5021)
                     /gene="dnaX"
                     /locus_tag="PKM49_05590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNY82321.1"
                     /translation="METLYRKYRPKDFEEVVGQVPIKRLFQRSIEAGALSHAYIFTGP
                     KGTGKTTFARIISKRVNCLAPLGSNPCNRCDQCLAIANGNHLDVIEMDAASNRGIDDF
                     RAIREKIAYQPVQGRKKVYIIDEVHMLTNEAFNAILKTLEEPPPHVLFILATTNPEKI
                     PETVLSRCQLIPFHNLSEAEIVAYLEKISRLEGSELKTDAAETIASFARGGLRDALVL
                     LEQSLRFAEPGQPITEMDVTKVIGGITRQEMEGWIETLLRNDSLEIVRFVEGCAQKGV
                     IWDTFLNQMMTALVARIQTTGKTQPYLQLGAAIAGLSKKMNRMEEKTAYLTLRFLEIG
                     AGFSPVSGIGTETAKEPAYERVLQSPLASELEGLIRKIGESDMAVAVALRLSKVNMKD
                     SSIEIDYTGCASVTSWVLRKKKEWVAAFLKEKGYEARWIEPSLKDPKTLTPEMKTYYQ
                     KLKKLFPNEEIRIIESE"
     gene            complement(5105..5752)
                     /gene="upp"
                     /locus_tag="PKM49_05595"
     CDS             complement(5105..5752)
                     /gene="upp"
                     /locus_tag="PKM49_05595"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HNY82322.1"
                     /translation="MKHEKWEQLRVVDHPLTKHKLSIMRDTQTGPKEFRELLYELTLL
                     LAYEATYCLKTHEVQIETPICRMVGEKIEDKDVAIVPILRAGLGMFEGVLTLLPNASV
                     GYIGLYRDAVTHLPIEYYCKIPVVREKDVFILDPMLATGQSAARAVDMIKRAGGTGER
                     IFFLSIISAPEGIEELISKHPDVNIFTAALDERLNENDYIVPGLGDAGDRLYKTP"
     gene            complement(5749..6219)
                     /gene="fliJ"
                     /locus_tag="PKM49_05600"
     CDS             complement(5749..6219)
                     /gene="fliJ"
                     /locus_tag="PKM49_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HNY82323.1"
                     /translation="MKRFTFRLQRFLDILIKRLDQKRIELGMISQELRRKEEEIAVQK
                     KKIFSTQAAFHQRMLSKQIKPSELTGYSLYIDGQYEALNELIRQRAQIEQRLVRCKEE
                     YIEINRNVQIFERLKEKKYRAFAEEADLEERKEIDEIAIRRVTRSNLLEAGDEP"
     gene            complement(6264..6857)
                     /gene="yihA"
                     /locus_tag="PKM49_05605"
     CDS             complement(6264..6857)
                     /gene="yihA"
                     /locus_tag="PKM49_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNY82324.1"
                     /translation="MPSHFSNVKLFRTVFNVKGLIKEGYAQFAFAGRSNVGKSTLLNY
                     LFDQKLAKTSSTPGKTRSVNYYLVNEQFFCVDLPGYGYAKASKSEQDLWRRTLESYFE
                     SNSALREVFILIDSRHLFLEKDEQMVVWLRHLGVPFSFVLTKTDKLTTTQLRKQVEAI
                     KDAHPQNRSVFLASSLKRTGRSELVAYIDDLQKALAE"
     gene            complement(6859..9279)
                     /gene="lon"
                     /locus_tag="PKM49_05610"
     CDS             complement(6859..9279)
                     /gene="lon"
                     /locus_tag="PKM49_05610"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNY82325.1"
                     /translation="MKKFSKLEEFAQEGKVTIDIPDQLPLIPMRTNMLVYPGSVIPFY
                     VGRDRSLNALEESIEKMNRLVFLVCQKNLEIDDPTQADLFDIGVVAQVMQLMKLPDGT
                     FKVLVEGLVRARKIRYVEGEKFDSAKIRIALPEYRVTKMIEVMVRKVTEDTQRYFNMT
                     KKLPPEALQALDDTSDVDRFADFVSSILPLPLEDKQKLLEAEHPKNRLEALMGYLAKE
                     IDLLKVEEDLERKVRFKLEQNQKEYYLREKMRTIKEELDGSEEQEIVELREKLEKGSY
                     PAFVKEKALHEIDRLEKSPTYSAESTVIRTYLDWLLGLPWNAETVDEINIKHARKILD
                     DDHFGLEEVKERVLEFLAARAFSKGLKAPILCLVGPPGVGKTSLGQSLARAMGRKFVR
                     LSLGGMRDEAEIRGHRRTYVGALPGRIIQLIKNSGVRNPVILLDEVDKMGQSFQGDPA
                     SALLEVLDPEQNVNFVDHFIEVPFDLSKVIFITTANVLYTLPPALKDRLEVIEIPGYT
                     ELEKLEIAEHYLIPKILAEHEMTKKIRFLPLAIRTVIKSYTREAGVRNLHRELASLVR
                     KSALKLLEEELPSIRIDRKTALEMLGAERFFEDERLERPYIGICTGMAWTAVGGSILH
                     IESLFVQGKGKLILTGQLGDVMKESAQIALSVARHYCSEENNEYFDTHDLHVHVPEGA
                     VPKDGPSAGITLAVSIASSILQKPVNHQIAMTGELTLRGKVLPVGGIREKVLAAYRAG
                     IKQVVLPKQNQKDMEKVPLEVKKDLRVHFVSHIEEVLKLLLLKGTQEKKSVRAVKTET
                     TTTPESGD"
     gene            complement(9322..>9782)
                     /locus_tag="PKM49_05615"
     CDS             complement(9322..>9782)
                     /locus_tag="PKM49_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82326.1"
                     /translation="TVALFLESLLPHSYVVKLGVRTERQENRIPLFPVDADFSDISDT
                     IPPDTEYLIIESGRIAKKIKPDCLIYMDLPEDAPTEDDGRAHGLEDPQRARKESTLWL
                     LDTADLVSGKKVSCQKAWEIADRLAIQKILMGKILDFLKIKVKGCQLGLF"
BASE COUNT         2065 a         2774 c         2330 g         2613 t
ORIGIN      
        1 gcggcgtaag cgccacttct tttcgcgacg gcgcgatacg cttttgcacg tgtcttgaaa
       61 aatagggttc acacggaggg cacagagaac acagagaaaa gattcttgtg aacgccccgg
      121 gagcgaggga ctttaattcg gtatggtctt ctgcttcgct ctcccaagcg ctcccggagg
      181 ggcgctaaat aggccgaaag gtcaacgccg ttttgaccaa atcggcgaag gactcctgca
      241 aactggcgcc accgaccaat ccgccatcga cgtccgggcg cgccatcagg ctcgcgaagt
      301 tttcaggctt gatactccct ccataaagga tcgaaacctt cgaggccacc tccgcgccgt
      361 acaggtcgct cagcttttct ctgattcgcg aacacgcctc ctgggcttgc caaggcaacg
      421 cgactttccc cgtgccgatg gcccacaccg gctcgtaagc gatggcgatc ttctccacat
      481 ccggggcggg aagccccttt aacccttccg tcacttggtt atccaacacc gacaaagtct
      541 ctccgctctc acgctggctc aaggtttcgc ctacgcagaa gatcggctta aggccggaag
      601 ccaaaagact tttgagtttc gtgttgatga aatcgttggt ttctttgaaa atcgctcgcc
      661 gctctgaatg accgacgatg acgtagcgga caccggaagc gacgagcatc cgaggggata
      721 tctctccggt gaaggcgcct tcttcggcgg ggtagacgtt ctgagctcct atggccaacg
      781 gtgttccgac gagaacatcg cgcaccgcgt gaaggtctac gtaaggaggg cacaagacca
      841 cttcgacggg ttcgaaagac ttcggtaaca cttgaagcaa acggcgggcg aacgcccccg
      901 cctcatcagg cgttttgtac attttccaat tacctgcgac gaccattttc cgactcttcc
      961 gaacgcatag gctcgccaca ccgggcattt ctttcccttc caacatctcg agcgaggctc
     1021 cgccgcccgt agaaacgtgg gtcacttctt cttccagccc gaatttggcg atcgccgctg
     1081 cgctgtctcc gcctccaacg acggttgtgg cgtccttccg ctccgatagc gccacagcga
     1141 tcgctcgcgt accttcctcg aattccggga gttcgaagac acccataggt ccgttccata
     1201 cgatcgtttt tgcgcctttc atcgcctctt tgaacaaagc gatcgttcgt tctccgatat
     1261 ccaatcccat ccatccgcga ggtaccccgg cttcccgggt gaccgtccgc ttgatttgac
     1321 cgctttccat cttttcggaa acgacaacgt ctatcggtaa caaaatttcg acggatcgtt
     1381 ctcccgattt ctcgaggagc gccttcgcca agggtatctt gtcttcttct accaaggagc
     1441 tgcccgtttc aaagccgagg gctttccaaa aggtaaacat catcgccccc ccgatcaaca
     1501 gcctatccgc tttggtcaga aggttttcga tgaccccgat tttatcggac actttcgcgc
     1561 ctcccaaaac ggccacatag gggtgctccg gcttctccac gagtttaccg aggaagcgga
     1621 gttctttttc catcaagaaa ccactatagg agggcagata atcggctacc ccgacgttag
     1681 aggcgtgcgc tctgtgggcg gtgccgaagg cgtcgttgat gagcagatcg ccataggtag
     1741 ccagctcacg ggatagccct ttgtcgttct tttcttctct cggatcgaaa cgggtatttt
     1801 cgagcaagag gacctctccg ttctgaagtt tcctcacttt ttgttcggat tcttctccga
     1861 cgaagacgct tgaaaataga accggtttct ccaacagcgc agatagcgct gaaaccaccg
     1921 gaagaaggct gtacttcgga tctggcccgc cttttggtct tccgagatgg gatagcagta
     1981 tcactctggc acctttctca agggcgtaac gtatggtcgg cagcgcggct cgtatccgtg
     2041 tgtcatccgt tacttggttc gttttcttat cgagcggaac attgaaatcc acacgcatca
     2101 acacgttttt cccggtgagt tccacatctc gtatcgtcaa tgcttccatt ctctcctcct
     2161 taaaaaaagg cacgccgaag cgtgccgatg tttttgcgac gactacattc ccttcatcat
     2221 caagccgacg agatcgacaa cgcggcaact gtatccgtat tcgttgtcat accagctaca
     2281 gatcttcagc aggtttccat ccatcaccgc agtcagcgtc gcgtcgaaaa ctccggaact
     2341 ggttaagcct atgatatcgg agctgacgat catttcttcg ttgtaatcga tgatcccttt
     2401 caacttcccg gagcaggcct ctttcatcac ccggttgacc tcttctttgg tcgtgggctt
     2461 ttccaggacg atcgtcatat ccgtaataga gccgtcactc acgggcactc tcaacgcgat
     2521 accatccagc tttcctttaa gctgaggaat cacgagtccg atcgccttag cggccccggt
     2581 ggtcgtaggt atcgtgttaa gcgccgccgc tctggctctt ctgaggtctt tatggggaag
     2641 gtcaaggatc ctctggtcgt tcgtgtaagc atgaaccgtg gtcaataacc ctttctgcac
     2701 gccaaaggtt tcgtgcagga ctttgacgat cggggcgatc gagttcgtgg tgcaagaagc
     2761 gttcgacacc actttcatct caggggtgag aatcccttcg ttacaaccga tgatgatcgt
     2821 cgcgtcgacc cctccttttt cgggagcggt gattaaaacc ttcttcgcgc ccgcatccag
     2881 gtgagcctgg cacttatctt tccccgtgaa cacgcccgtc gactcgatca ccatctcggc
     2941 ccccagcgct tttaatggca gatctgccgg gtttttctgt gcggtgacct taatagactt
     3001 cccgttcacc ttgataaagt cctctccgtg ttcgaccgtt ccaccgaaag tccggtgcac
     3061 ggagtcatac ttgaacaaat acgccaaggt cttcgcatcg gtcaaatcgt tgatgagtac
     3121 gacctcgtaa cctccccgtt taacgatttc gcgtaagacg agccttccaa ttcttccgaa
     3181 tccattgatc gcaataggca ctttaggcat acgccacctc catcagattt gtatcaaatt
     3241 cctaatccac caattcccaa ctgagttttc aactgctcga gctcagcgtc tttttccttc
     3301 gacacagttt ctatcgcctg gttgaacgcc gcgctcagca aatcggtcaa ttcctctttc
     3361 tcttctaaaa gaccatcttc taactcgatc gcgctaatgt ggaagtcacc cttcatcgtg
     3421 atcttgatgg caccaccgcc agcggttatc tcgaaagatt tttgcgcgaa actctcttcg
     3481 atttcttgca tcgtctcgcc cgcttgattg atttttgtca taaggtcttg catagagggt
     3541 tttcctgaag tggatgatgg ccgtaaggat tttcctccga aactccgcat cttttttgcc
     3601 attctgactc ctttcttcta ttcgctttcg ataatgcgta tttcttcgtt aggaaacaac
     3661 tttttcaatt tttgatagta agttttcatc tccggcgtca gagtcttcgg gtcttttaat
     3721 gagggctcta tccaccgggc ctcgtacccc ttctctttca ggaacgcagc gacccattct
     3781 ttcttcttcc gcagtaccca agaggttacg gaagcacagc cggtatagtc tatctcaatt
     3841 gaagagtcct tcatattcac tttgctcaat cttaaagcaa cggcgacggc catgtcggat
     3901 tctccgatct ttcggatcaa cccttctagt tccgaagcca gcggagactg aaggacacgc
     3961 tcgtatgccg gttctttcgc tgtctccgtc ccgataccgg aaaccggaga aaaacccgcg
     4021 ccgatctcca aaaaccgcag ggttaaataa gcggttttct cctccatccg gttcatcttt
     4081 ttggacagac ccgcgatcgc cgctcccagc tgcagatagg gctgcgtttt tccggtcgtt
     4141 tgtatccgcg cgaccaacgc cgtcatcatc tgattcaaaa acgtatccca tatcacgcct
     4201 ttctgggcgc atccttccac gaatcgcacg atctcgagcg aatcgttccg cagcaacgtc
     4261 tcgatccaac cttccatttc ttggcgcgtg atcccgccga taactttcgt gacgtccatt
     4321 tccgtgatcg gctgccccgg ttcggcgaac cgcaaggatt gctccaacaa gaccaaggca
     4381 tcgcgcagcc cgccccgggc gaagctcgcg atcgtttcgg cagcgtcggt ctttagttcc
     4441 gaaccttcca ggcggctaat cttctccagg tacgccacga tctccgcttc gctcaggttg
     4501 tgaaacggga tcagctggca gcgggaaagc acggtttcgg gtattttttc gggatttgtg
     4561 gtcgccaaaa tgaacagtac gtgtggaggc ggctcttcca aggtcttcaa gatagcgttg
     4621 aaggcttcgt tcgtcagcat atgcacttca tcgatgatat agaccttttt tcgcccctga
     4681 accggttgat aggcgatttt ttcgcggatc gcgcgaaaat catcgatccc tcggttcgaa
     4741 gccgcatcca tctcgatgac gtccaaatga ttcccattcg caatggccag acactggtcg
     4801 catcgattac agggattgct gcctaagggg gccaaacagt tgacccgttt cgagatgatc
     4861 ctggcgaagg tggtcttgcc ggtccctttt ggaccggtga aaatataggc atgtgaaagc
     4921 gccccggctt cgatggatcg ttggaacaac cgcttgatcg gtacctgacc gaccacttcc
     4981 tcgaaatcct tcgggcgata ctttcgatac aatgtctcca taaaagccct ctccggttgc
     5041 tgaaccgaat cgatctattc gtacaaatcg atcatcgctc ggttattata tcacaacctt
     5101 tttcttaggg agttttatag agtcgatctc ccgcatctcc caacccggga acgatgtagt
     5161 cgttttcgtt gagccgttca tccaaagctg ccgtgaaaat attgacgtca ggatgtttgg
     5221 agatgagctc ttcgatccct tcgggagcgg agataatcga taaaaagaag atgcgctcgc
     5281 ctgtcccgcc cgcccgcttg atcatatcaa ccgctctggc ggcggattgt cctgtggcga
     5341 gcatcgggtc cagtatgaac acgtctttct ctcggactac cggaattttg cagtaatact
     5401 cgatcggcag gtgggtaacc gcgtctcggt agagcccgat ataccctacc gaagcgttcg
     5461 gcaagagggt taaaacgcct tcgaacatcc ccaatcctgc gcgaaggatc ggtacgatcg
     5521 caacgtcctt gtcttcgatc ttttctccta ccatacggca tatcggggtt tcgatctgga
     5581 cttcgtgggt ttttaaacaa taagtggctt cataggcgag cagtagcgtt agttcgtata
     5641 ggagctctcg aaactctttt ggaccagtct gggtgtctct cataatcgac agtttgtgtt
     5701 tcgtcagcgg atggtctacc accctcagtt gctcccattt ctcgtgcttc acggttcatc
     5761 acctgcttct aaaagattgc ttctcgtcac tcttctaatg gcgatctcat cgatctcttt
     5821 tcgttcttct aaatccgcct cttcagcgaa ggcacgatat ttcttttctt tcagccgctc
     5881 gaagatttgc acgtttctgt tgatctctat gtactcttct ttgcatcgta ccaagcgttg
     5941 ctcgatctgg gcccgttgtc gtatcagctc gttcagggct tcatattggc cgtctatgta
     6001 gaggctgtac ccggtcaact cgctcggttt gatttgttta gagagcattc gttgatgaaa
     6061 ggccgcctgg gtagaaaata tcttcttctt ttggacggcg atctcctctt ctttccttcg
     6121 gagctcttga gatatcatcc cgagctctat cctcttctga tccaaacgtt tgatcagaat
     6181 atcaagaaac cgctgtaacc ggaacgtaaa acgcttcagc cgctatcccc tcctcacgga
     6241 ggcgaggcgc ctcgcagccg ccttcattcc gccaaggcct tttggaggtc gtcgatatag
     6301 gcgacgagct ccgacctacc ggttcgtttc agagaagagg cgagaaagac agaccggttt
     6361 tggggatgag cgtcttttat cgcctctacc tgttttctga gttgggtggt cgtgagtttg
     6421 tccgttttcg tcagaacgaa agagaagggg actccgaggt gccgaagcca cacgaccatt
     6481 tgttcgtctt tttccaggaa tagatggcga gaatcgatca gtataaagac ctctctcaac
     6541 gccgaattgc tctcgaaata gctctcgagt gtccttctcc acaggtcttg ctcggatttg
     6601 gaagctttcg cgtacccgta gccgggtaaa tcgacacaaa aaaactgttc gttaaccagg
     6661 taatagttaa ccgaacgggt cttgccggga gtcgagctcg tcttggcgag cttttgatcg
     6721 aacagatagt tgagcagggt cgactttccc acattcgaac gtcccgcgaa ggcgaactgc
     6781 gcatagcctt ctttgatcag ccctttgacg ttgaataccg ttcgaaagag cttgacgtta
     6841 gaaaaatgtg aaggcacctt agtctcccga ttcaggggta gttgtcgttt ccgttttaac
     6901 cgcgcgaaca gactttttct cctgcgttcc tttcaacaag agaagcttca atacttcttc
     6961 gatgtggctc acgaaatgaa cacggaggtc ttttttcact tcgagcggaa ccttttccat
     7021 atctttttga ttctgcttcg gcaggacgac ctgtttgatg cccgctcggt aagcggccaa
     7081 caccttctcg cggatacccc cgaccggcag taccttccct ctcagggtca gttctcctgt
     7141 cattgcgatc tggtggttca cgggtttctg caagatcgag gaggcaatag aaaccgccag
     7201 ggtgatgccg gcggatggcc catccttcgg aacggcgcct tcaggaacgt gaacgtgcag
     7261 gtcgtgagta tcgaaatact cgttgttttc ctcactgcag tagtgcctgg caacggaaag
     7321 agcgatttgc gcggactctt tcatcacgtc tccgagctgg ccggtgagga tcagtttccc
     7381 ttttccttgg acgaagagcg actcgatgtg caggatcgat ccgcccaccg cggtccacgc
     7441 catcccggta caaataccga tgtagggtct ttccaaccgc tcatcctcga agaacctttc
     7501 cgcgcctaac atctccaaag cggttttccg atcgattcgt atcgatggca gttcctcttc
     7561 gaggagtttc aacgcgcttt ttcgaacgag gctggcgagt tcccgatgga ggtttcgaac
     7621 accggcctct cgtgtgtatg atttgatgac cgttcgaatc gctaaaggca aaaaccggat
     7681 tttttttgtc atttcgtgtt cggcgagaat cttcggaatc aggtaatgtt cggctatctc
     7741 cagcttttcg agttcggtgt acccggggat ttcgatcact tccaaccggt ctttcagcgc
     7801 ggggggcagg gtgtaaagga cgttcgccgt ggtgatgaag atgacctttg aaaggtcgaa
     7861 gggcacctct ataaaatgat cgacgaagtt gacgttttgc tcgggatcga gcacctctag
     7921 caacgctgaa gctgggtcgc cctggaaact ctgtcccatc ttgtccacct catccagtaa
     7981 gatcactgga ttgcgaaccc cgctattttt gatgagctgt ataatacgcc cgggaagggc
     8041 gccgacgtaa gtacgtctat gacctcggat ctccgcctcg tcacgcatcc cgccgaggga
     8101 aagccgaacg aactttctcc ccatcgcgcg cgccaaggac tgtcccaacg aggtcttgcc
     8161 cacacccgga ggtccgacca gacagaggat cggcgctttt aaacccttgg aaaaggcgcg
     8221 ggccgctaag aactctaaca ctctctcttt aacctcttcc aaaccaaaat ggtcgtcatc
     8281 caagatcttg cgcgcgtgtt tgatgttgat ctcatcgaca gtctccgcgt tccaaggcaa
     8341 cccgagcaac cagtcgagat aggtccgtat caccgttgat tctgccgaat acgtggggga
     8401 cttttccagc cgatctatct catgcaaggc tttctctttg acgaacgcgg ggtaagaacc
     8461 cttttcgagc ttctctcgca gctcgacgat ctcttgttct tcgctgccat cgagctcttc
     8521 tttgatcgtt ctcatctttt cgcggagata gtattccttt tgattctgct ccagtttaaa
     8581 gcggaccttc cgctcgaggt cttcttccac ttttaagaga tcgatctctt tggccaggta
     8641 acccatcagc gcttccaacc tattcttcgg gtgctcggcc tccagcagct tttgcttgtc
     8701 ttccagcgga agcgggagaa tggacgagac gaagtccgcg aaccgatcga cgtccgacgt
     8761 gtcatcgaga gcttgtaatg cctcgggggg tagctttttc gtcatattga agtatctttg
     8821 cgtgtcttct gtgactttcc ggaccatgac ttcgatcatt ttcgtcacgc gatactccgg
     8881 aagggcaata cgtatcttcg cagagtcgaa tttttcgcct tccacgtacc ggattttccg
     8941 ggcccgcacc aacccttcca ccaagacttt gaaggtgccg tccggcagtt tcatcaactg
     9001 catcacctgt gccacgaccc cgatatcgaa aaggtccgct tgtgtcggat catcgatttc
     9061 aaggtttttt tggcacacta aaaaaacgag tcgattcatc ttctcgatcg actcttccaa
     9121 ggcgttcagc gacctatccc tacccacgta aaagggtatg accgatccgg ggtagaccag
     9181 catattggtg cgcatcggga tgagcggtag ctgatctggg atatcgatcg taaccttccc
     9241 ctcttgagcg aattcctcca atttagaaaa cttcttcatc cgtttctctc actcactcct
     9301 tctctatctc tatctctatc tctaaaacag gcccagctga caacctttca cttttatttt
     9361 caagaaatcc aatatcttcc ccataaggat cttctgaatc gccaagcggt ctgcaatctc
     9421 ccaagccttc tgacaggaga ccttctttcc gcttaccaga tccgccgtat ccaacaacca
     9481 cagcgtcgac tccttccgtg ccctctgcgg gtcttctaag ccgtgcgctc ttccatcgtc
     9541 ctcggtaggg gcgtcctccg gaagatccat atagatcagg caatcgggct taattttttt
     9601 tgcgattcgc ccgctttcga taatcagata ctccgtatcc gggggtattg tatcagaaat
     9661 atcggaaaaa tccgcgtcaa ctggaaagag gggaatccgg ttttcttggc gttctgttct
     9721 tacccccaat ttgactacat atgagtgtgg taaaaggctt tccaaaaaga gggccacggt
     9781 tg
//
LOCUS       DAOPXN010000075         9751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7022_length_9751_cov_5.058065, whole genome shotgun sequence.
ACCESSION   DAOPXN010000075 DAOPXN010000000
VERSION     DAOPXN010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9751
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7022_length_9751_cov_5.058065"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            335..859
                     /locus_tag="PKM49_05620"
     CDS             335..859
                     /locus_tag="PKM49_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82327.1"
                     /translation="MMKKCLCTAVILVAIVSALFASVLVEDFRSIRSFEYKITSPDGV
                     FYYGARVEKKGNVNELTTFARRTLDASEEIKMEEIFSTSFGNWFILFLNPIFNAALES
                     VDLQNPMTLSFFGMQLKYEGTEAVGRWTGKKYTLYGSGKPVITWVIDEKIKMSIKTVL
                     HDEGLTLELVNFEM"
     gene            complement(952..2619)
                     /locus_tag="PKM49_05625"
     CDS             complement(952..2619)
                     /locus_tag="PKM49_05625"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNY82328.1"
                     /translation="MKKTLWILFLCAMAILSFGAVKELQPQKIIIVPDLPAALTVTLS
                     LNKPEGSVYQPGELITMTVVTNKDAYVVIYDTEANGRTTILFPNLYQPDNFVKANQPL
                     SIPQGYKLKIGNEIGKEYVQVVASTKQFATYNQWAKVFSATNPFLEATKDAESELQKL
                     IQKIIIVPDTPQPEWNSFSTFFYVGQKPQIPATVDFTSSPSGAQIMLDGNWIQKTTPY
                     KAIMTEGWHFVRYTLPGYRTHEENFYLSQGMVKLIHATLIPETPPVAVLNVNSLPPGA
                     NIFLDGALKGSTPMTVQNITPGTHVLRLTLRGFQPFEQPVPFVAGQTQTLNITLVPEV
                     VILKGTLTLNVTPADATIYVDGVEYQVFGGRASLSLDAGNHTLSVSRRDYESQTIPFT
                     IFGNQLTTLNVTLLPSKGTMEISSDPSGAAIYINGSDTGYRTSATGKLTMTIDPGTYE
                     FRLRKDGYQDWVQTKTISPGVNAPLHVILQPNMAKLTVAANTGAMLYIDGKPYGTVSV
                     GQPFSLNVPPGNHEILLVKEGYYSFISIFLMETGKTYDLNPYLTPIY"
     gene            complement(2769..3149)
                     /locus_tag="PKM49_05630"
     CDS             complement(2769..3149)
                     /locus_tag="PKM49_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82329.1"
                     /translation="MKRKRLIVQLLLILALVGVSVALFVIGKGHQLLIDNKRATIDGK
                     RVEAYEWVNVYLDGATKPIELEEDDRQMGKVSGPWHNIRIEILENEKVVKTYERRFTV
                     GLANSFILSIQKMVAEKDDWLTPR"
     gene            complement(3162..4259)
                     /locus_tag="PKM49_05635"
     CDS             complement(3162..4259)
                     /locus_tag="PKM49_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82330.1"
                     /translation="MKTTKKTSYKHFLIAYAVPIMFITLSIFTIPLSGFSPEYLIQEM
                     IIRLARNSFLVLALLIPIIAGMGLNFGMVLGAMAGQIGLILITDWNIVGIPGMVLAMI
                     ISIPLSTFLGWIAGHILNRAKGREMVTSYILGFFVNGIYQFIVLYMMGKAIPIGNPAL
                     VLSRGYGIRNAINLEGIRQTLDNLILLKIGKVQIPVATFILISAFCLFIIWFRKTKLG
                     QDMRAVGQDMEVAEAAGIPVNRTRIISMIISTVLAGFGQIIFLQNIGTLNTYNSHDQT
                     GMYSIAALLIGGATVSHATIPNIFLGVVLFHLMFVTSPMAGKNLIGSAMLGEYFRLFI
                     SYGIIAISLVLYAWRRQKEKERARMDLRGNT"
     gene            complement(4256..5299)
                     /locus_tag="PKM49_05640"
     CDS             complement(4256..5299)
                     /locus_tag="PKM49_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82331.1"
                     /translation="MSWIQSFVKQAGLPRVIIGLFLLSLFVAAPFVGVSVTSSINDVL
                     VRFGMNAVMVLAMVPMVQSGCGLNFGLPLGIIAGLLGATLSLELGVVGILGFFVAVGI
                     SIPFSIVFGYFYGQLLNRVKGDEMVIATYVGFSSVAFMNILWLVLPYRNPTMVWGFSG
                     EGLRTTISVEGYWLSALNDFLSFQLGTHIKVPTGMFLFFAFLCFLIWAFFHTKTGTAM
                     TAVGSNPEFARASGVNVDRSRTQSVILSTVLGGIGILVYEQSFGFIQLYNGPFYMAFP
                     AVASILLGGASVHKATLLNVIIGTILFQGIVTMTPSVINSMIQTDMSEVIRIIATNGM
                     ILYALTRKSVVKS"
     gene            complement(5296..6930)
                     /locus_tag="PKM49_05645"
     CDS             complement(5296..6930)
                     /locus_tag="PKM49_05645"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNY82332.1"
                     /translation="MDKNAVLLRCEHISKSFYDNRVLDDINFELREGEILGLVGENGA
                     GKSTLMKILFGMSVIAQTGGYEGTVYVGDKPVKFDSPFEALEEGIGMVHQEFSLIPGF
                     TATENILLNREVTHHNFLVEAFGERLSTLNMPEMIERGDKALNTLGVAISPQMLIAEM
                     PIGHKQFTEIAREVDRQNTKIIILDEPTAVLTETEADVLLSSLKKLAEKGIGIIFISH
                     RLQEILEVADRIIVLRDGNLVKEVDAAHADVIKIAEWMVGRKMDASAFADKDVNTNPE
                     VLLEVKNLWVDMPGETVRDVSFEVHRGEILGIGGLAGQGKIGIPNGIMGLFPAGGIVK
                     FRDEVVPLNNPRQVLNLGMAFVSEDRRGVGLLLEETLDWNISFTAVQVKEWYVKKYLG
                     GLIKWRDDAAIRQLTSEYIKTFDIKCLGGHQLAQELSGGNQQKVCLAKAFALKPELLF
                     VSEPTRGIDVGAKKLVLDTIRNSNRERGMTIVMTSSELEELRSVCDRIMIVDEGRVAG
                     ILPATAPSTEFGLLMLGTGETDRREGEIHNGKVKTK"
     gene            complement(7037..8215)
                     /locus_tag="PKM49_05650"
     CDS             complement(7037..8215)
                     /locus_tag="PKM49_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HNY82333.1"
                     /translation="MKKLLLVLLISVVAVLAFSAAPFHIGIVTGTVSQSEDDLRGAER
                     LIQEYGDVTAGGMIKHITYPDNFMAEMETTISQIAGLADDPLMKAIIVNQAIPGTTEG
                     FRRVREKRPDILLLAGEPHEDPIVIESAADLAINSDFVARGYLIIWSAKKMGAKTFVH
                     ISFPRHMSYETLSRRRAIMEAACKDLGLRYVFETAPDPTSDIGIAGAQQFILEKVPAW
                     VEKYGKDTAFFCTNDAHTEPLLKQLLTYGGMFVEADLPSPLMGYPGALGIDLSSAAGD
                     FPKIMSTVEAEIVKKGGAGRFGTWAFSYGYTTTAGLGELAIRVLKGEASLTRLPDLMA
                     AYNKFTPGAAWNGSFYVDANTGIRARNHILIYQDTYVFGQGFLGSANQEVPEKYLDIR
                     "
     gene            complement(8558..9379)
                     /locus_tag="PKM49_05655"
     CDS             complement(8558..9379)
                     /locus_tag="PKM49_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82334.1"
                     /translation="MRGKTRNVGIYVILLIFAFFFLLPVYMLLATSFKPLVEIGMGTM
                     WLLPKQPSLEGFTDSIQRLSPNLWNSFLLVIPATIFSCLLGSINGYIFSKIRFPGINL
                     LFLFVLFGMFIPYQSILFPLIQFLQKTQLYGTIWGLIITHVIYGLPITSLIFRNYYME
                     VPDEMLEAAGIDGAGLFKSYFLILFPLSIPGFVVAVIWQFTNIWNEFLFAVTITSDPK
                     KQPITVALVNLAGSQMIQWNLQMAGAIVAAIPTLLVYIFLGKYFIRGLMSGSVKG"
     gene            complement(9381..>9751)
                     /locus_tag="PKM49_05660"
     CDS             complement(9381..>9751)
                     /locus_tag="PKM49_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82335.1"
                     /translation="GYTMAMYLAGLRAIPESMTEAARVDGANAWQTFWKVKWPFLRPI
                     TLSAMIILGHISLKIFDLVYSMTGSGPKNVTDMPSIYMFEKTFRSNRYAEGSAIAVIM
                     LLMVAVVIIPYLISTLKKSD"
BASE COUNT         2339 a         2504 c         2487 g         2421 t
ORIGIN      
        1 atgactctcc cggaaaaaaa gaaaagacat gccggactaa agtccggcgt tccgagggcg
       61 tttctatgtg ttctctctgt gcactctgtg ttctctgtgc ggcacttctt atttattttc
      121 agaaagacgt gccgaactga agttcagcgt tcccagggag gatcgcaaga actaaaagag
      181 tatcgcgaga tttcgtgggc ctttttttcc tccccctcct ccgcttccct tttttgctgc
      241 acctcgatac tatacacgca cgctgtttac aaaaattctc cgtgttgtgg tacaatctca
      301 gcggcttttg agcgagatta aagggggggg gacaatgatg aaaaagtgtt tatgtacggc
      361 ggttattctt gtggcgatcg tgtcggcgct ctttgcgagc gtgttggtcg aagacttccg
      421 gagcattcgt tctttcgagt acaagatcac gtctccggat ggcgtttttt attacggtgc
      481 gcgtgtggag aagaaaggca acgtcaatga attgacgacc tttgcgaggc gaaccctaga
      541 cgcatcggaa gagatcaaaa tggaagagat cttttcgact tcattcggga attggttcat
      601 cctgtttctg aatccgattt tcaatgcggc gctcgagtcg gtcgaccttc aaaacccgat
      661 gacgctgagt ttttttggta tgcagctcaa gtatgaggga acggaggcag tcggaagatg
      721 gactggtaag aagtacacgt tgtacggatc cggaaagccg gtgataacgt gggtcatcga
      781 cgaaaaaatc aaaatgtcca tcaagacggt cttacacgac gaggggttga cgctcgagct
      841 cgtgaacttc gagatgtaat cacgaagtct tgaaccgcct tttccacgag gggctatgcg
      901 cgcccgaaaa aaagcgccgc gaccgcggcg cttttttatt ttcgtttgcg tttaatatat
      961 cggcgtaagg tacgggttga ggtcgtaagt ctttccggtt tccatcaaga agatggagat
     1021 aaaggagtaa tacccttctt tcaccagcag gatttcatgg tttccgggag gaacgttgag
     1081 cgaaaaaggc tgaccgacgg agacggttcc gtaaggtttc ccatcaatgt agagcatagc
     1141 gccggtgttc gcggcgaccg tgagcttggc catattgggc tgcaagatga cgtggagcgg
     1201 cgcgttcacg cccggtgaaa tggttttggt ttgcacccaa tcctgatacc cgtcttttct
     1261 gagtcggaac tcgtaagttc ccgggtctat cgtcatcgtc aatttccccg tggcgctcgt
     1321 ccgatatcca gtgtcgcttc cattgatgta gatggcagct ccggacggat cagaagagat
     1381 ctccatcgtg cccttcgagg gtaatagcgt gacattcagc gtggtcaact ggttcccgaa
     1441 gatcgtgaac gggatcgttt gcgattcgta atcccttcta gaaacggaga gggtgtggtt
     1501 ccccgcgtcg agcgataggg aagctcttcc gccaaacact tggtactcca cgccatccac
     1561 atagatcgtg gcatccgcgg gggtgacgtt gagggtgagc gtccctttca gaatcacgac
     1621 ctcagggacg agcgttatgt tgagggtttg tgtttggccc gctacgaacg gaacgggctg
     1681 ctcgaacggc tgaaagccgc gcagcgtcaa acgtagaacg tgggttcctg gggtgatgtt
     1741 ctggaccgtc atcggcgtgc tccctttgag cgcgccatcc aagaagatgt tggctccggg
     1801 aggtaaggag ttcacattta atacggctac gggcggggtt tccgggatca gagtggcgtg
     1861 tatcagtttg accatccctt gagaaagata aaaattctct tcgtgggtcc tgtaccccgg
     1921 taacgtgtag cgcacgaaat gccaaccttc cgtcataatg gccttgtagg gagttgtctt
     1981 ctgtatccag ttgccatcca gcataatctg ggcgcctgaa gggctcgacg tgaaatcgac
     2041 ggttgccggg atctgtggtt tttgtccaac atagaagaag gtgctgaagg aattccattc
     2101 cggttgcggc gtgtctggta cgatgataat cttttgtatg agcttctgca gttcggattc
     2161 ggcgtctttg gtggcttcca agaaggggtt cgtcgccgag aagacctttg cccattggtt
     2221 ataggtggcg aattgtttgg tgctcgccac gacttgcacg tactcttttc caatctcgtt
     2281 tccgattttc aacttgtatc cttgcgggat cgacaagggt tggttcgctt tcacgaaatt
     2341 atcgggctga taaaggtttg ggaacaaaat ggtggtcctc ccgttggctt ccgtatcgta
     2401 gatgaccaca taagcgtcct tattcgtcac caccgtcatc gtgatcagtt cgccgggctg
     2461 ataaacggag ccttcgggtt tattcaaaga tagcgtaacc gtcaaagccg ccggcaggtc
     2521 tggcacaatg atgatcttct ggggctgcaa ttctttgacg gctccgaaag acagaatcgc
     2581 cattgcacat aagaaaagta tccacagggt ttttttcata gtcttgcctc cttcgattcg
     2641 ttcttatcga taaagacgga attggcgcgg gtttatcaca ccgaacaagg tcgtcacgct
     2701 tcccggagta tgacggtcgt gtatcggcgt cagtggagct gttcgcggct ttatgtcgcg
     2761 aatggccgtt aacgtggagt caaccaatca tccttttccg cgaccatctt ttgaatactc
     2821 aggataaaag aattcgccag tcccacggtg aaccttcttt cgtaagtttt caccactttc
     2881 tcattttcta agatctcgat gcgtatgttg tgccaaggac cactgacttt gcccatttgc
     2941 cggtcatctt cttcaagctc gatcggcttt gtcgcgccgt ccaaatagac gttaacccat
     3001 tcgtaggctt ccacccgttt cccatcgatc gtcgcccgct tgttatctat gagcaattgg
     3061 tgacctttcc cgataacgaa caaggcgact gaaactccca cgagggccaa aattaaaagc
     3121 aattgaacga tcaatcgttt tctcttcatc gcgccctcct cttaggtatt tcctctgagg
     3181 tccattcggg cccgttcttt ttctttttgt ctccgccacg catagagcac caaggaaatt
     3241 gcgatgatcc cgtaagagat gaaaagacgg aaatattccc ctaacatcgc ggaaccgatg
     3301 aggttcttac cggccatagg cgaggtgacg aacatcaaat ggaagagcac aacgcccaag
     3361 aagatgtttg gaatcgtggc gtgtgagact gtagcgcctc ctatcaagag ggccgcgatc
     3421 gagtacatgc cggtctggtc atggctattg taggtattga gcgtgccgat gttctggaga
     3481 aagatgatct gaccgaaccc ggccaatacc gtagaaatga tcattgagat gatgcgggtc
     3541 ctgttcactg gaatccctgc cgcttccgcg acttccatat cctgaccaac cgcgcgcata
     3601 tcttgtccca gttttgtttt gcggaaccag atgatgaaaa ggcaaaacgc cgagatgagg
     3661 ataaaggtcg caactggaat ctgtaccttc cctatcttaa gaagaatcag attgtccagt
     3721 gtctgacgaa taccctcgag gttgatcgcg ttccgtatcc cgtagccccg tgagaggacg
     3781 agcgctgggt ttcctatcgg aatggccttc cccatcatat atagcacgat aaactggtaa
     3841 ataccattta cgaagaatcc taagatataa gaagttacca tctctctacc tttcgccctg
     3901 ttgagaatgt gtcccgctat ccacccaaga aaggttgaaa gaggtatcga aataatcatc
     3961 gctaaaacca ttcccggtat gcctacgatg ttccagtcgg tgatgaggat caaaccgatc
     4021 tgccccgcca tcgcgccgag gaccatcccg aagtttaggc ccatgcccgc gatgatcgga
     4081 atcagtaggg cgagaaccaa gaaggagttt ctcgccaagc ggatgatcat ctcctgaatc
     4141 aggtattcgg gagaaaaccc tgaaaggggg atcgtgaaga tgcttagagt gatgaacata
     4201 attgggaccg cataagcgat caaaaagtgt ttataggagg tttttttggt tgttttcatg
     4261 acttcaccac cgactttctc gttaaggcgt acaagatcat cccgttcgtg gcgatgatcc
     4321 ggatgacttc ggacatatcc gtttgtatca tgctgttgat gaccgaaggc gtcattgtaa
     4381 caatcccttg aaagagtatc gttccgatga taacgttaag gagtgtggcc ttatggactg
     4441 aagcgcctcc caacaggatg gaggccaccg cgggaaaagc catatagaac ggtccgttgt
     4501 agagttgtat aaaaccgaag ctttgttcgt agacgagtat tccgatgccg cctaaaaccg
     4561 tcgagaggat aaccgattgg gttcgcgagc ggtcgacgtt cactcccgag gctctggcaa
     4621 actccggatt cgacccaaca gcggtcatcg cggttccagt ttttgtatgg aaaaacgccc
     4681 atatcaaaaa gcataagaaa gcaaagaaga ggaacattcc ggtgggcact ttgatgtgcg
     4741 tccccagttg aaaagagagg aaatcattca gcgcggacaa ccaatagcct tcgacactga
     4801 tggtcgttct gagaccttcg ccgctgaatc cccaaaccat cgtggggttc ctatagggta
     4861 agacgagcca caggatgttc ataaaggcga ctgaagaaaa cccgacgtag gtcgcgatca
     4921 ccatttcatc ccctttgact cgattgagca gctgtccgta gaagtatccg aacacaatgg
     4981 aaaaaggtat tgaaatccct acggcgacga aaaatcccaa gattccgacc acgccgagct
     5041 ccaagctcag cgtcgcgcct aacaatccgg ctatgatgcc tagagggagc ccgaagttca
     5101 gtccgcaccc ggattgaacc atcggcacca tcgccagaac catcaccgcg ttcataccga
     5161 agcgcacgag cacgtcgttg atcgatgaag tcacgctgac tcccacgaaa ggagcggcca
     5221 cgaacaacga aaggagaaac aacccgatga tgacccgcgg taacccggct tgtttcacga
     5281 acgattggat ccagctcact ttgttttcac cttcccgtta tggatttctc cttctcgccg
     5341 atcggtttca cccgtgccga gcatcagcag accgaattcg gtggacggag cggttgccgg
     5401 tagaattccc gcgactcttc cctcatcgac gatcataatt cgatcacaga cggaccgcag
     5461 ctcttctagt tcagatgagg tcatcacgat ggtcataccg cgctctcggt tgctgtttcg
     5521 aatcgtgtcc aagacgagtt ttttcgctcc cacatcgatg cctcgcgttg gctcggagac
     5581 gaacagaagc tcgggtttca acgcgaaggc tttcgccaag cacacctttt gctggtttcc
     5641 gcccgagagc tcctgcgcca gctgatgtcc gccaaggcat tttatatcga aagtcttaat
     5701 gtattcgctc gttaattgcc tgatggcggc atcatcccgc catttgatca agccgcccag
     5761 gtactttttc acgtaccact cttttacctg aaccgccgtg aacgagatat tccagtcgag
     5821 ggtttcttct agcaacagcc cgactcccct tctgtcttcg gaaacgaacg ccatcccgag
     5881 attcaatact tggcgcgggt tattcaaggg cacgacctca tcgcgaaact taactattcc
     5941 gcccgccgga aagagtccca taatgccgtt cgggataccg atcttccctt gtccagccaa
     6001 cccgcctatg cccaatattt cacctcggtg gacttcaaag gacacgtctc gaacagtttc
     6061 tccgggcata tcgacccata agttttttac ttctaacaac acttcggggt ttgtatttac
     6121 gtctttgtct gcgaaagcgg aggcgtccat cttcctgccg accatccatt cggcgatttt
     6181 gattacgtcg gcgtgagcgg cgtcgacctc cttgacgagg ttaccgtccc tcagaactat
     6241 gatccgatcc gccacttcca agatctcctg taaacggtga gagatgaaga tgatgccgat
     6301 tcctttctcc gctaactttt tcaacgaaga gagcaggacg tcagcctccg tttccgttaa
     6361 aacggccgtt ggttcatcaa ggatgataat tttcgtattc tggcggtcca cttcgcgcgc
     6421 gatctccgtg aactgcttat gcccgatggg catttcagcg atcagcattt ggggactaat
     6481 agccactccc agcgtgttca gcgctttatc tccgcgctcg atcatttcgg gcatattaag
     6541 agtcgagagc cgttctccga aagcctcaac aaggaaatta tgatgggtga cttctcgatt
     6601 caacaggatg ttttcagtgg ccgtaaaccc cggtatcaaa gagaattcct ggtggaccat
     6661 gccgattccc tcttcaaggg cctcgaatgg agagtcgaac ttgaccggct tatctcccac
     6721 gtataccgtc ccttcgtatc cccccgtttg ggcgatcacc gacatcccga acagaatttt
     6781 catcaacgtc gatttaccgg ccccgttttc tcccactaac cctagaatct caccctcacg
     6841 aagctcaaaa ttgatatcgt cgagtacccg gttatcatag aaactcttgg agatatgctc
     6901 acaacgcaac agtacggcgt ttttatccat aactttcacg ttcggggttt gcacgttttc
     6961 acctctgtta ttccaacgaa agaattaaaa ataagggaac tttacgttcc cttatctttc
     7021 ttacttgctc ttcggcttac cgaatatcga ggtacttctc cggaacctct tggtttgcgg
     7081 aaccgaggaa gccctgaccg aagacgtagg tatcttggta gatcaagatg tgattgcgag
     7141 ctctaatacc ggtgttggca tcgacgtaga aactcccgtt ccaagctgcg cctggagtga
     7201 acttgttgta ggcggccatc aaatcgggaa gcctggtcag agacgcctcg cccttgagca
     7261 cacgtatcgc gagttcaccg agaccagcgg tggtggtgta gccataggag aaggcccacg
     7321 ttccgaatcg gcccgctccg cctttcttca cgatttcggc ttcaaccgtg ctcataatct
     7381 tcgggaagtc tcccgcggcg gaggaaaggt caatacccaa cgcgccgggg tagcccatca
     7441 acggagaagg caggtcagct tccacgaaca taccgccgta agtcagcagc tgtttcagca
     7501 agggctctgt gtgcgcgtca ttcgtacaga agaacgcggt atctttgcca tacttttcta
     7561 cccaagcggg tactttttcg aggatgaact gctgcgctcc ggcgataccg atgtcactgg
     7621 tggggtccgg agcggtttcg aatacgtacc tcaagccgag gtctttacaa gcggcttcca
     7681 taatggctct tcttctggaa agggtttcat agctcatatg tctggggaag gagatgtgga
     7741 cgaaagtttt cgcgcccatc tttttcgccg accagataat cagatatcct ctggccacga
     7801 agtcagagtt gattgcgagg tcagcggccg attcgataac gatcgggtct tcgtgcggtt
     7861 ctccggccag taagagaatg tccggtcgtt tttcgcgcac tctcctgaaa ccctcggtcg
     7921 ttcccggtat cgcttggttc actataatgg ctttcatcag cggatcatcg gccaaacccg
     7981 cgatttggga aatagtcgtt tccatctctg ccataaagtt gtccggataa gtgatatgct
     8041 taatcatccc accggcagtc acatcgccgt actcctggat cagcctttcg gccccgcgca
     8101 ggtcgtcttc agactgagat actgttcccg tcacgattcc gatgtggaac ggtgcggcgg
     8161 agaaagcaag aacggccaca acgctgatca aaagaaccag tagcagtttc ttcacgtaaa
     8221 tcgccctcct caaagatgtt ttgccagatc ggcaccgaga ttatagcaca gatgttaccc
     8281 tcaatcaagt gctctcgtga gcacaatata tgagcggatt actaaagatt atcccataga
     8341 aatgaagaaa ttgggacgaa cgccggcgac gccggggaaa aaatgaagca tatgccgagc
     8401 gtaagttcgg cctgttttat ggttttcggg aacgccttca gggcgccccc aggcgctccc
     8461 aggcgttcca aaaaaaaagc cttaaaaaag gctttttggg tatttgcggt gatcggacga
     8521 aataacccca atgaacccct tgaagtgccc ccccacatca accttttacg gagccggaca
     8581 tcaggccccg tatgaagtat tttcctaaaa atatgtagac cagcagtgtc ggaatcgccg
     8641 cgacgatcgc gcccgccatc tggagattcc attgtatcat ctggctccca gcgaggttca
     8701 cgagcgcgac ggttatcggt tgcttctttg gatcgctggt gatcgtcacg gcgaataaga
     8761 actcgttcca tatattcgta aattgccaga tcacggcgac gacgaacccc ggaatggata
     8821 aggggaacag gattaaaaag tagcttttaa acaaacccgc gccgtcgata ccggcggctt
     8881 ccagcatttc gtccggcact tccatatagt aattccggaa gatcaaggag gtgatgggta
     8941 ggccgtagat cacgtgcgtg atgatcaatc cccaaatcgt cccgtaaagc tgtgtctttt
     9001 ggaggaattg tatgagcggg aacaagatgc tttgataggg gatgaacatc ccgaacaaca
     9061 cgaacagaaa caataggttg atcccgggaa aacggatttt tgaaaagatg tacccgttga
     9121 tggaacccag gaggcaagag aagatcgtcg ccggtatcac caataggaaa ctgttccata
     9181 agttcgggga caaccgttgg atcgaatcgg tgaagccttc cagggacggt tgtttcggaa
     9241 ggagccacat cgtgcccatc ccaatctcga ccaacggctt gaagctggtc gccaagagca
     9301 tatagacggg tagaaggaaa aagaaggcaa aaatcagcag aatgacgtat attcccacgt
     9361 tcctggtttt tccacgcaca tcaatcactc tttttcaacg tggagatgag ataggggatg
     9421 atgacgacgg ccaccatcag gagcatgatg accgcgatcg ccgatccttc cgcgtaacgg
     9481 ttggaccgga aggttttttc gaacatatag atagagggca tgtcggtgac gtttttcggg
     9541 ccgctgcccg tcatcgagta aaccaaatcg aagattttca aggagatatg cccgaggatg
     9601 atcatggcgc tcagcgtaat cggcctgaga aagggccatt tcactttcca aaaggtctgc
     9661 cacgcgttgg cgccatccac ccgcgcggct tcggtcatac tttccgggat cgcgcgcaac
     9721 ccagccagat acatggccat cgtgtacccg g
//
LOCUS       DAOPXN010000076         9683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7078_length_9683_cov_4.806294, whole genome shotgun sequence.
ACCESSION   DAOPXN010000076 DAOPXN010000000
VERSION     DAOPXN010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9683
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7078_length_9683_cov_4.806294"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PKM49_05665"
     CDS             <1..634
                     /locus_tag="PKM49_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HNY82336.1"
                     /translation="RILTPLLVLVVLILLQKYAAFYIPVLGLPLTFVLSAIAVLLVNP
                     KKTSFKRWMTVISNTMEQVFPLLATVISVGALVNILTGTGVRGLIAITFVTLPLGLIY
                     ALALLFTPFAQGSLSYGSAVILGTPIIFLFNNLGFNVTVVTAALSLMFPLGDCLPPSR
                     IVGRLSIEAVQYEGSYMSFLKEIMVPAFFMAAVALIMFIFPNQLSFLVVY"
     gene            651..824
                     /locus_tag="PKM49_05670"
     CDS             651..824
                     /locus_tag="PKM49_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82337.1"
                     /translation="MVILNEIIHILYFVLTAVICIFLIWNLFKRTKKTGWVYDIVYAY
                     VIITFILRVLMIK"
     gene            838..2175
                     /locus_tag="PKM49_05675"
     CDS             838..2175
                     /locus_tag="PKM49_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacylase"
                     /protein_id="HNY82338.1"
                     /translation="MKNTPLWFKLLALALIAIMGFIAGKEFYELRHYQESVVVSPAFT
                     KKLMLSDYLPSLKGTWGDTPIYLFDSGVPGGSALILGGSHPYEPATMLTAYMVAENIS
                     VEKGKVYVAPVANRSANTLGMLGNAYPMFYHLKTPFGTKQYKIGDRNTNPLDQWPDPF
                     VYVHYPSGQNLAYQDLRNFNRTWPGKADGSLTERISYAFMELVRNEKIDIFIDYHEAS
                     LMYPVVDTYVAHDFSMDIAILAQMNLDTFKYPMKAEASPKKLRGLSHREVGDYSIDPE
                     LAKELYAAEEFTNAKNDYKEKLKIIEKSSHQTLVVLMETLEPFIERVAGRIDEDLMLV
                     GLDEFIQTAADKRLLFAKYDTRTGITMPIDMDGRGGYVVKGAPMYYRVARHLEGTLEV
                     IEMMNLLFPERELLISYPGFHDIMEAGSKKGNDAIGDFLHDPAKSDPKRVFYQ"
     gene            2219..3886
                     /locus_tag="PKM49_05680"
     CDS             2219..3886
                     /locus_tag="PKM49_05680"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNY82339.1"
                     /translation="MWKLKTVLTVLLVIVILSSVGFTCTIIAVGKMATVDGSAIITHN
                     DDSSVADFRLWIQRGGKWQTGAMRPIVIDSHDYIDYSKFPIVDISKNRNGRAIQLGED
                     PQPPVTNDYFHSRYSFMNDWGVAMGESTAGIATNNDYGKAVRKALFEDDEGIVDCWAA
                     QDIALERAKSAREAVQIMGDLIDRYGWNVTGGGGELINITDGNEVWVVEFYGANLWMA
                     IRMPDDMVYVGANRMRFQVDPRDGIDPVSGAKYEEILTSANFIDYAVEKGWYDPASGK
                     KFRPAEVYANRIDSHNKREWRVLDMVAPSLGLEDEIGEYPLFVKPDRLLSVHDIFLIS
                     GDHYEGTKWDLTKGVGAGPWGDPLTNYGIGGRQRPIGIPLTCYLHIAQVKEWLPREFR
                     GISWYGYGSVGHSYLTPLFPAMEKLPDFYSVGSRYEKFRRDSGWWVNTYVQEMVGMRY
                     SEAIKELRAFREPKMASVYAQTAQLQEYAAMMYRQGNPKEAVKLMSDFCYHTAVGWNQ
                     DWLDLGDRLFTKYATERINFGSAGYPTWWQDWLNPAKNPELLKYANK"
     gene            3912..5570
                     /locus_tag="PKM49_05685"
     CDS             3912..5570
                     /locus_tag="PKM49_05685"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HNY82340.1"
                     /translation="MKKSTLVKLLVVALLLVYSVSFTCTILGVGNKAMADGSTVITHN
                     DDSTVADFRLWKIPAADWPEGAMRDIVLDGHDYIDYGNFPKSVEYQKNRTGYAMVVGQ
                     IPQVAHTYSYFHSRYSFMNEKGVAISESTGGVDRSLPNGPALIKYYSDNEGIIDCWMA
                     QDIALERASSAREAVLIMGALIEEYGWSNVGGGPESMTIGDGKDVWLFEAYGADLWVA
                     IRIPDNAFTVTANRLVIMEVDPDDHANVLYAKNLFRTAVEQGWVSPSLPKNQWKLAEI
                     YSPNNSLYATRREWRALTLVAPKFMAENGYDSHQLRYPVYVVPEKPLTLQDIWNIKAD
                     YYQGTEYDLTVGPAAGPWGDPIRYANSSSTERTATWERSINMHRTCYLHIGQTKAWLP
                     DPIKGISWYGYGAPDTAYLVPLWPAMESLPLFYKTGNRFEDFRRDSGWWVSSYVQQMA
                     ELRYSSAIKDIRAFRQPKMDMLYIVTPQVQEIAAQMYRSGNDKGALALIHNYAYQNAV
                     AMFNEWLKLGDWLLGNYALGYNNFKTTPYPQWWNDLVGFTTPKP"
     gene            complement(5894..6448)
                     /gene="efp"
                     /locus_tag="PKM49_05690"
     CDS             complement(5894..6448)
                     /gene="efp"
                     /locus_tag="PKM49_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNY82341.1"
                     /translation="MEVGNLRKGDVIVLEGQIYRAVEVNKHKVAMGRGMVRARLKNVV
                     TGLIFEKTFTSGGNVEEATLTMKSVEYLYNDGGLYYFMDLSDYDQIPVDEEFVGDAKD
                     FMVENIQMSLLYHGDTLLGVQLPNTVVLTIVETEPNFKGDTVSGGGKPAILETGYKTT
                     VPMFVETGEKIKVDTRTGDYIERA"
     gene            6645..7679
                     /locus_tag="PKM49_05695"
     CDS             6645..7679
                     /locus_tag="PKM49_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNY82342.1"
                     /translation="MKAIILAGGVGERFWPISTAKTPKQFLKLFGHESLIKQTFERLK
                     GFLKPRDIYVITSCRYIEKTVKELPELPVTNIIGEPERKNTAPACYIGTALAKEGELV
                     LTVPADHYIYPQEDFRATVEKGIDLLTEQEALLTIGIKPERAETGYGYIEAVKQAEHY
                     RVIRFHEKPKAETAEKYLTSGRHFWNSGMFLWRKEVFMKEMAQHAREIHAVLLSVDST
                     SPEQLAQAYPLLPAISIDYALMEKSNCILMIPASFHWSDMGNWESIRELEGYSMSEEH
                     LVLHHSENVFVRSTSTRPIILLGARDLFVIDTEQGLLVAQKDHLPDLREAIRKMRERP
                     SQEDPEKSQQ"
     gene            7879..9543
                     /gene="groL"
                     /locus_tag="PKM49_05700"
     CDS             7879..9543
                     /gene="groL"
                     /locus_tag="PKM49_05700"
                     /inference="COORDINATES: protein motif:HMM:NF009487.0"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNY82343.1"
                     /translation="MAKQFKFDHEARLSLEKGMNVVADAVKITLGPRGRNVVLEKSWG
                     SPTITNDGVSIAKEIQLEDKFENMGAQLLKEVASKTNDIAGDGTTTATVLAQSIAREG
                     LKNITAGTNPMLMKRGIQEAVKEVEKYIKSHSKKIETKHDITQVATISANNDDEVGSL
                     ISEAMDKVGQDGVITVEDSKTTKTLVEFTEGMKFDRGYISPYFVSDPEKMEYVANEPY
                     ILITDKKISAIKPVIPILEKVAQTGKPLIIIAEDVEGEALTTLVLNKIKGTLNVCAVK
                     APGFGDRRKAMLEDIAILTGGTVISDEVGLSLEKIELHYLGHSGSVRVKKDDTLVIDG
                     KGKAVKIKERIGQIKKQIEATTSEYEKENLQERMAKLSGGVAVIKVGAATETELKEKK
                     HRIEDALSATRAAVEEGIVAGGGVTLLRAKKKVEALAQTLEIPEEKVGATIVAQALET
                     PLRLITQNAGIDGSIVINKILENDNPNYGYDALKNEYCDLIASGIIDPAKVTRSALLN
                     AASITAMLLTTEVLVTDKPEPKPATPQMPPNYGGMGGMGGMGGMGY"
BASE COUNT         2470 a         2479 c         2567 g         2167 t
ORIGIN      
        1 caggatccta acccctctac tcgtcttggt ggtcttgatt cttctccaaa agtacgccgc
       61 gttctacatc cccgtactcg gtttgccgtt gaccttcgtt ctttctgcga tcgccgtttt
      121 actcgttaat ccgaaaaaga cttcgttcaa gcgatggatg acggtgattt cgaacacgat
      181 ggagcaggtt ttcccgctgt tggcgacggt tatcagtgtc ggggccctcg tcaatatcct
      241 gaccggaaca ggggtgcgag gtctgatcgc catcaccttc gtcacccttc ctttaggttt
      301 gatctacgcg ttggctttgc tgttcacacc tttcgcgcaa ggttctttga gttatggaag
      361 cgcggtcatc ctcggcacgc cgatcatctt cttgttcaac aatctcgggt tcaatgtcac
      421 cgtggtaacg gctgcgttga gcttgatgtt cccgttggga gactgcctgc cgccttcccg
      481 tatcgtcggc cgattgagca ttgaagcggt gcagtacgaa gggagttata tgtcgttttt
      541 gaaagagatt atggttccgg ctttctttat ggcagcggtc gcgctgataa tgtttatctt
      601 ccccaaccaa ctgagctttt tagttgttta ttgatcgggg gggctgaaaa atggttatcc
      661 ttaacgaaat catccacatc ctctactttg tcctaaccgc tgtgatctgt atctttttga
      721 tctggaatct cttcaagcga acaaaaaaaa caggatgggt ctatgacata gtctatgctt
      781 acgtgatcat caccttcata ctccgcgtcc tgatgatcaa gtaaggggct gagacgtatg
      841 aaaaacacac ctctatggtt taagctactg gccctggccc tgatcgcgat tatggggttt
      901 attgcgggga aagagttcta cgagctccgg cactatcagg agtcggtcgt cgtctctccc
      961 gctttcacta aaaagctcat gctcagtgac tatctgccga gtctgaaagg gacatggggc
     1021 gacacgccga tctatctctt cgattcgggc gtaccgggcg ggtcagcgct aattttgggc
     1081 ggttctcacc cgtatgaacc ggccacgatg cttaccgcct atatggtcgc cgaaaatatc
     1141 tccgtcgaaa aagggaaggt ctacgtcgcc cccgtggcca ataggagcgc caataccctc
     1201 ggaatgctcg ggaacgccta cccgatgttc taccatttga aaactccttt cggaacgaag
     1261 cagtacaaga tcggagaccg caacaccaat ccgctcgacc agtggccgga cccgttcgtt
     1321 tacgtccatt atccctccgg acagaacctg gcctatcaag accttcggaa ctttaaccgc
     1381 acgtggcctg gcaaagcgga tgggtcgctc acggaaagaa tctcctacgc tttcatggaa
     1441 ttggttcgga atgagaagat cgacattttt atcgattatc acgaagcctc tttgatgtac
     1501 cccgtggtcg acacctatgt cgcgcacgat ttctcgatgg atatcgcgat tttggctcag
     1561 atgaacctgg acaccttcaa gtacccgatg aaagcggaag cttctccaaa aaaacttaga
     1621 gggctttccc accgagaagt cggggactac agcatcgatc ccgagctcgc gaaagaattg
     1681 tacgcggccg aagagttcac caatgccaaa aacgactaca aagagaagct gaaaattatc
     1741 gagaaatcca gtcatcaaac acttgtcgtc ctgatggaaa cgttggaacc tttcatcgaa
     1801 cgtgttgccg gaagaatcga cgaagacctg atgttggtcg ggctcgacga gtttatccag
     1861 actgccgccg ataagaggtt gctgttcgcg aagtacgaca cgagaaccgg aatcacgatg
     1921 ccgatcgata tggacgggcg aggcggctac gtcgtgaaag gcgcgccgat gtattatcgg
     1981 gtcgccagac acttggaagg aaccttggaa gtcatcgaaa tgatgaactt gttattcccc
     2041 gagcgcgagt tactgattag ctacccaggc tttcacgata tcatggaagc cgggagtaaa
     2101 aaaggtaacg acgcgatcgg agatttctta cacgatccgg ccaagtccga cccaaaacgg
     2161 gtgttttatc agtaagccga actagagtta ccagaaaagg aaagggagac aagagagtat
     2221 gtggaagctt aaaacggtgc tgacggtact tttggtgatc gttattcttt ccagtgtagg
     2281 gtttacgtgc acgatcatag ctgtcggaaa aatggccaca gtggacgggt ccgccattat
     2341 cacgcacaat gacgactcct ctgtcgccga tttccgccta tggattcaac ggggtggaaa
     2401 atggcagacc ggggcgatgc gacctatcgt gatagactcc cacgactaca ttgactacag
     2461 caagtttccg atcgtcgata tctcgaagaa tcgcaatgga agggctattc aactgggaga
     2521 agatcctcag cccccagtca ccaacgatta cttccattca cgttattctt ttatgaatga
     2581 ttggggtgtc gcgatgggag agtccacggc cggtattgcg acgaacaacg attacgggaa
     2641 agcggtacga aaagccctct tcgaagacga cgaagggatc gtggattgtt gggcggcgca
     2701 ggacatcgct ttggaaaggg ctaaatctgc acgtgaagcc gttcagatta tgggtgatct
     2761 gatcgaccgg tacggctgga acgtcacggg tggcggtggg gaactgatca atatcaccga
     2821 tggaaacgaa gtctgggtcg tggagttcta tggtgccaac ctgtggatgg cgattcgtat
     2881 gcccgacgat atggtgtacg tgggcgcgaa ccggatgcgt ttccaagttg acccgcgcga
     2941 cggcatcgac cctgtttctg gcgcgaagta cgaagagatt ctcacctccg cgaattttat
     3001 cgactacgcg gtagaaaaag gatggtacga tcccgcttcc gggaaaaaat tcagaccggc
     3061 tgaggtctac gccaaccgta tcgattctca caacaagcgg gaatggcgcg ttctggatat
     3121 ggtcgcccct tctttaggac tggaagacga gattggggag tacccccttt tcgtgaaacc
     3181 cgaccgcttg ctgtccgtcc acgacatttt cctgatctcc ggagaccatt acgaaggcac
     3241 gaaatgggat ctgacgaagg gcgtaggcgc gggaccctgg ggtgacccgc tcaccaacta
     3301 cggaataggc ggccgacaaa gaccgatagg gattccgttg acctgctacc tccatatcgc
     3361 ccaagtgaaa gaatggctac ccagagagtt cagaggaatc agttggtacg gttacggttc
     3421 ggtgggccat tcttacctga ccccgctctt tccggcgatg gaaaaactac cggacttcta
     3481 cagcgtcggt tccagatacg aaaaattccg gcgggattcc ggctggtggg tcaacactta
     3541 cgttcaagag atggtcggta tgcgctacag cgaggcgatt aaagaattga gggccttcag
     3601 agagcccaag atggccagcg tctacgcgca gacagcgcag ctacaggaat atgcggcgat
     3661 gatgtatcgc caaggcaacc cgaaagaggc cgttaagttg atgagcgatt tctgttacca
     3721 tacggctgtc gggtggaacc aggactggtt ggatcttgga gataggttgt tcactaaata
     3781 cgccacagaa agaatcaact tcggttctgc cggttacccc acgtggtggc aagattggct
     3841 gaacccagcg aaaaaccctg aacttttgaa gtacgcgaat aagtaaaaca ccttaaaaac
     3901 ggagggaaaa catgaagaaa agcacgttag tcaaactgtt agttgtagcg ctgttgttag
     3961 tgtactcggt ctccttcact tgtaccattc tcggtgtggg aaacaaagca atggcggatg
     4021 gatcgaccgt tatcacccac aacgacgact ccaccgtcgc ggatttccgg ttatggaaga
     4081 tcccggcggc tgattggccc gagggcgcga tgagagacat cgtgttggac gggcacgatt
     4141 acatcgatta tggcaacttc cccaaatcag tcgagtatca aaagaaccgc actggttacg
     4201 ctatggttgt aggtcagatt ccccaagtcg cccataccta cagctacttc cactcccgct
     4261 attcctttat gaacgagaag ggcgttgcca tctccgaatc caccggaggc gtggaccgat
     4321 ctcttccaaa cgggccggcg ctcattaagt actactctga caacgaaggg atcatcgatt
     4381 gctggatggc gcaggatatt gcgttagaga gagcctcttc cgcgagggaa gccgttctga
     4441 tcatgggcgc cctcatagag gaatacggat ggtccaacgt cggtggcggt cccgaatcga
     4501 tgaccatcgg cgacggaaaa gacgtctggt tgttcgaagc ctatggagcc gacctctggg
     4561 ttgccattcg catccccgac aacgccttca ccgtaaccgc caaccgattg gtgattatgg
     4621 aagtcgaccc cgatgaccac gccaacgttc tgtatgcgaa gaatcttttt agaaccgctg
     4681 ttgaacaggg ttgggtaagc ccgagtcttc cgaaaaacca atggaagctc gccgaaattt
     4741 attctccgaa caacagcttg tacgcgacca gaagagaatg gagagcttta accctcgttg
     4801 ctccgaagtt catggccgaa aacggctacg attctcacca actccgctac ccggtgtacg
     4861 tcgttccgga gaaaccactg acgcttcagg acatttggaa cattaaagcc gactactacc
     4921 aaggcacgga atacgacctc accgtcggcc ctgccgccgg tccttgggga gacccgatcc
     4981 gctacgccaa ctcctcgtcg actgagagaa cggctacctg ggaaagatcc atcaatatgc
     5041 accgaacctg ctacctccac atcgggcaaa ccaaagcgtg gctgcctgat ccgatcaaag
     5101 ggatcagctg gtacggatac ggcgcgccgg ataccgccta cctcgttcct ttgtggcccg
     5161 caatggaatc cttacctttg ttctacaaga ctgggaaccg atttgaagat ttccgcagag
     5221 actccggctg gtgggtgtcc tcgtatgtcc agcagatggc ggagctgaga tactccagcg
     5281 cgatcaaaga catccgcgct ttcagacaac cgaagatgga tatgctctac atcgtgaccc
     5341 cgcaggttca ggaaatcgca gctcagatgt atcgaagcgg aaatgataaa ggcgccctcg
     5401 cattgatcca caactacgcc taccagaacg cggtagcgat gttcaacgaa tggctgaaat
     5461 tgggagactg gctcctgggc aactacgctc tcggatacaa caacttcaaa accacgcctt
     5521 atccgcaatg gtggaacgat ctcgtcggct tcaccacgcc gaagccataa gcgctgcttg
     5581 attcagggtt ttcgagcata ccgggtttgt gcccggtatg ctctttttat aggcgttccc
     5641 agggcgctct cagaaaagtc ggagttaaaa ggcgtctttc cataaaaaaa cgggcgtaac
     5701 tcagcccgtg gttcacaact gatactttga acgtggtgag ggcaaccggt cgaaacgccc
     5761 gcacgtcttt tttcgtttaa tgcgcgatca tatcatttcc gcttgtcctg acgcctccca
     5821 attcggggat cgctttcttt gctcgcgaat tctgggcttt ctcaaaggta agcctaagtg
     5881 gaacaaacgt ctattaagcc ctctcaatgt agtcgccggt tcgcgtgtcg accttgatct
     5941 tttcgcccgt ttccacgaac atcggcacgg tggtcttgta cccggtctct aagatcgcgg
     6001 gttttcctcc tccggaaacg gtatctcctt tgaaattcgg ttcggtttcg acgatcgtca
     6061 agacgacggt gttgggcagc tgtacgccca agagggtgtc tccgtggtag agcaggctca
     6121 tctgtatgtt ttccaccatg aagtctttcg catctccgac gaactcttcg tcgaccggga
     6181 tttgatcgta gtcgctcaaa tccataaaat agtaaagccc gccgtcgttg tacaaatatt
     6241 cgacagattt catcgtcaat gtcgcttctt caacgttccc gccgctcgtg aaggtctttt
     6301 cgaagataag ccccgtcacc acgtttttca aacgcgcccg gaccatcccc cgccccatcg
     6361 ccactttgtg cttgttcact tccacggcgc gatagatctg cccttcgagg acgatgacgt
     6421 cccctttcct taaattgccc acttccatgc tgtgcgcacc tcctgtatcg acaccttggg
     6481 tcattttcgg cagttattat aacatatctc ccctgcggat gaggagagcc gcccgtttcg
     6541 gagcgatttg ggtaggccgg tcacggtcaa atgcgcccgc gtttttcgtc tacctttcga
     6601 ggtttaaggt ataatcaagg tatcgaggat aggggaggga taagatgaaa gcgatcatat
     6661 tagccggggg agtgggggag aggttttggc cgatatcgac ggccaagacg ccgaagcaat
     6721 ttttaaagct gttcggtcat gaaagcctta tcaagcaaac cttcgagcgc ctgaaagggt
     6781 tcctcaagcc gcgggatatt tacgtgatca catcctgtcg ctatatcgag aaaacggtga
     6841 aagagctgcc ggaattgcca gtcaccaaca tcatcggtga accggagcgg aagaatacgg
     6901 cgccggcctg ctatatcgga accgcgttgg ccaaagaggg tgagctcgtc ctcaccgtac
     6961 cggccgatca ctacatctat ccccaagagg attttcgagc gaccgtcgaa aaggggatcg
     7021 acctcctcac cgaacaggag gccttgctca cgatcggtat aaaacccgag cgggcagaaa
     7081 ccggttacgg gtatatcgag gctgtgaagc aagcggagca ctatcgggtt atccggtttc
     7141 acgaaaaacc taaagccgag actgccgaaa agtacctgac ctccggccgc catttttgga
     7201 acagcgggat gtttttgtgg cgcaaagagg tttttatgaa agagatggcc caacacgcgc
     7261 gagaaatcca cgcggttctc ctatcggtcg actcaacctc gccggagcaa ttagcccagg
     7321 cttacccgct tcttccggct atcagtatcg attacgcgct aatggaaaaa tcaaactgta
     7381 tcctgatgat acccgcgtcc tttcattggt cggatatggg caattgggaa tcgatacgag
     7441 aactcgaggg atattcgatg agcgaggagc atctcgtctt gcaccattcg gaaaacgtct
     7501 tcgtgcgttc gacctcgacg aggccgatca tcttgttggg cgcgcgcgat ctcttcgtga
     7561 tcgatacgga gcaaggcctg ctcgtcgccc agaaagacca tttaccggat cttcgcgagg
     7621 cgatccgcaa gatgagagag cgcccatcgc aagaggatcc tgagaaaagc caacaataaa
     7681 cagagtaaaa cggtgtaata gatagcaata ataagaaaga aatcgatatt cgagaataaa
     7741 tggtacatga gaagcgagat caggaattcg gccgtttgta acgaaggccc cctttttagt
     7801 atgatacgtt agcacttcgg gagacagagt gctaatggat gtccatacta tagtagataa
     7861 catacaggag gtgccttcat ggcgaaacag tttaaatttg atcacgaagc gaggctttct
     7921 ttagaaaaag ggatgaacgt cgttgcggat gcggttaaga tcaccctcgg cccgcgtgga
     7981 agaaatgtgg tgctcgagaa aagctggggc agcccaacga tcaccaacga tggggtgtcg
     8041 atagccaaag agatacaact cgaagataaa tttgagaata tgggcgcgca gctcctcaaa
     8101 gaggttgctt cgaagacgaa cgacatcgct ggagacggca caacgacggc gaccgtttta
     8161 gcccaatcta tcgcgcgaga agggttgaaa aacatcacgg caggcacgaa tccgatgttg
     8221 atgaagcgcg gaatacagga agccgtgaaa gaagtggaaa agtacattaa aagccatagt
     8281 aaaaaaatcg agacgaaaca cgacatcacc caggtggcca cgatcagcgc caacaacgac
     8341 gacgaagtgg gcagtttgat ttcggaggcg atggacaagg tcggacaaga cggggttatc
     8401 actgtagagg actccaaaac gacgaaaacc ctcgttgagt tcaccgaagg gatgaagttc
     8461 gatcgcgggt acatctcccc ctacttcgtt tccgatccag aaaagatgga gtacgtggcg
     8521 aatgagccct acatcctgat tacggataag aagatttcgg cgatcaagcc cgtcatcccg
     8581 atactggaaa aggtcgcgca aacgggcaag cccctcatca taatcgccga agatgtggaa
     8641 ggggaagcgt tgaccacgct ggttctcaac aagatcaaag gcacgctcaa cgtatgcgcc
     8701 gtgaaagcgc ccggattcgg tgacagaagg aaagcgatgc tcgaagatat cgcgatcctg
     8761 accggcggca cggtgatcag cgatgaggtc ggcctttcac tagagaagat agagctacac
     8821 tacctgggcc attccggatc cgtccgcgtc aaaaaagacg ataccctcgt gatagacgga
     8881 aaaggaaagg ccgtcaagat caaagagcgt atcggtcaaa tcaaaaagca aatcgaagcc
     8941 accacttccg aatacgagaa ggagaacctt caggaacgga tggcgaaact ttccggcggt
     9001 gtggccgtga taaaagtagg agccgcgacc gaaacggagc ttaaagaaaa gaagcaccgt
     9061 atcgaggacg cgttgagcgc gactcgggcc gccgtagaag aaggaatcgt tgcaggaggc
     9121 ggcgtcaccc ttctacgcgc aaagaaaaag gtggaagccc tcgcccaaac cctcgagata
     9181 ccggaggaaa aggtgggagc gacgatcgtc gcccaagcct tggagacacc cttacgcctg
     9241 ataacccaga acgccgggat agacggctcg atcgtcatca ataaaatctt ggaaaacgac
     9301 aatcccaact acggatacga tgccttgaaa aacgagtact gcgatctcat cgccagcggg
     9361 atcatcgacc cagcgaaagt gaccaggagc gcgctgttga acgcagcgtc catcacggcg
     9421 atgctgttga ccaccgaggt cttggtgacc gataaacccg aacccaaacc cgctacccct
     9481 caaatgcccc ccaattacgg cggtatgggt ggtatgggtg gtatgggcgg tatgggatac
     9541 taaggacgag ttttcaaaag aggcgcgaaa gcgccttttt ttatgggctt gacttttccc
     9601 ctcttttgtt ttataattag ttatctactt aacccaactt tcgactaaaa gtgctacaaa
     9661 cggcgataaa agccactttc tca
//
LOCUS       DAOPXN010000077         9669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7088_length_9669_cov_4.678178, whole genome shotgun sequence.
ACCESSION   DAOPXN010000077 DAOPXN010000000
VERSION     DAOPXN010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7088_length_9669_cov_4.678178"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PKM49_05705"
     CDS             complement(<1..809)
                     /locus_tag="PKM49_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HNY82344.1"
                     /translation="MSLEKYLKYLRTDRLPHVWCPGCGNGIALKAIVGAIDELRLDPN
                     KVAVVSGIGCSSRATGYLHFNTLHTLHGRAIAFATGVKLARPDIKVIVITGDGDLAAI
                     GGNHFIHAARRNIDLTVVCFNNNIYGMTGGQYSPTTPRESYATTSPYGNFENAFDLSR
                     MAIAAGASYVARSTTYHFLPTQNFVMRGLENSGFSFIEVITQCPTYLGRYAGIGGAPE
                     MMLHFKEKALNLPKRKVTEEGKTEEENILNDPIVRLQEAQRNNPGRIIIGE"
     gene            950..2341
                     /gene="lpdA"
                     /locus_tag="PKM49_05710"
     CDS             950..2341
                     /gene="lpdA"
                     /locus_tag="PKM49_05710"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNY82345.1"
                     /translation="MTYDLIVVGSGPAGYTGAIRARQLGLRTAIIEMDKPGGVCLNVG
                     CIPTKALIHKAKEFRAVEGLKAHGLQVDASRFDFSGVTQYAKIVSTKLSKSVAFLLKK
                     AGVDTINGRAKFISPTTLEVSRGEEREVLTGRHFLIATGSRPKNLPGLVIDEERILSS
                     TGALFLKQLPKRIAIIGAGAIGVEFAYIYRSFGSEVDLIEVLPRILPLEDSEAVDTLT
                     KRYIKLGIQVLPSTRVLEAQPIEEGVRLTIQKQDGAQETLTVDKVLLAVGRTLNTDQL
                     GLENIPLTTERGFLRVNSRYQTAVPHIYAAGDVIPTPLLAHVASEEAINAVESIVDPQ
                     KEPIDFSGLIPSCIYSEPELASFGDQEAQLVSQNRPFKKTVFPYRGIGKSVATDQPEG
                     FIKLLSDPETERVLGATIVGAQATELIHEYMLLKRTGQPIRMLAHMIHAHPTLSEGMM
                     EAAKLALGEAINL"
     gene            2338..2721
                     /locus_tag="PKM49_05715"
     CDS             2338..2721
                     /locus_tag="PKM49_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82346.1"
                     /translation="MKPFHCTLVVFLLAATLACAWTTDLKTIKTRYTQEQIVALFEQA
                     RQYEWLEEYQEKDHAVYILTSKFLYRARLLDDDTLVFDQIKSRYYYDMQVPFWTIILV
                     FLISGVALFLIFFLPSDWRFRKKKP"
     gene            2782..5604
                     /locus_tag="PKM49_05720"
     CDS             2782..5604
                     /locus_tag="PKM49_05720"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HNY82347.1"
                     /translation="MNKRHLSGVCLFIALLTISFCQYVLKPGDQVEVYNYAYPELSQE
                     ALVGKDGYIQLKLIGAVKAEGMTLEELAAFAKNKLLAFAPESRVTVLLKNVYPIKAYI
                     IGEVYASTAIDLTYEDNPTLLKALSFCGGVKPTADLKNILLRHDDGTFDTIDISATLE
                     YGRDVAPTSIRSGDVIVVPRKYERRVYVFGSLEQSGVVYFEEKEPMVLEALLSKIKYD
                     LGTCEPTIALIRDGHETTLRLEAAFPQERKTALESNDIVLVRTLQPRYLYVTGLESAN
                     RVDFQREEEMTLQTLLGKLGIGPVSLLSLRIIPLKGAEKTVRIDRTVPYYLFETGDIV
                     EFPPQNYVYVSGNVGVTGKIDFPYSESFTVERLMAIIGVGEKEKGLLTIINPDGQRFL
                     YRYPEQISSRTPLERGAYVQFPPRRYVFLAGAVSENVERQLYFPNNEEMTLKHALAKA
                     SVDLSLYSYSITVIRNQEQYLFDLKSALLSEGEFFLETGDILYFEKDTDRYVHLVVSD
                     GESRKYSFRPEDPLDLFTLLIKYEKTGALTGDQIKIIDPSGRVFLSSVKSVLNHEENY
                     ELSPGSLVVINANERRVFVLGNAQEGGEILFGRDEPFTLTTLIAKCHPVLDERTQSVI
                     IQQGGKVTVLDIREALDVTATFPLQKDAVIYFKPYEPRIVTAFGQVRNPGLLSFGARE
                     PVNLMTVLSKAGGLLSDAGQRVKVIGEDGKIVTHNLLTEPNLERLSIDSGSYVVVESN
                     LDQHIAILGDVRTPGLYYLRDEKVSLLELLSQRGGVSDWTLNTTLELTRASGEKSLIN
                     IDLDPSALSTTMLRAGDTVFVFPSSRLKVYVFGAVVNPGIVPYRSKMTLLEALLYCRG
                     PSASAYMKKLMIFPGGVDANPVLLDYSGFRKEQGVVDYFLEPGDVIYVPQSALVEIKD
                     IAAFLGTMLTLVNSGFTLMDRLQSLP"
     gene            5631..7799
                     /locus_tag="PKM49_05725"
     CDS             5631..7799
                     /locus_tag="PKM49_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HNY82348.1"
                     /translation="MSSFEREELIDNELNELTLNDLFRMFRRRLGWFFLIVIITIVAT
                     AFYAFLSPKVYEASVTIKLTAATSAGVSSLLQGLPFGIGGVVSGGSVTTEIELISSRS
                     NIEQVIRNLNLLDILIPPEKRKPQESEQALMVRAIDALSDQISVNPVKNTNIVKITVS
                     FSEPELAARIANELAKVYNANLYDISKTQAREKLKFIETQLPISKAALDLSIQKVREY
                     KEAKQIFLISEEAKALLDVIVDFDKRLNAKKMELQTTQAGIAVIREKLSATNEKLISS
                     ETISINPLVSELRSKLTNLNIELAALEKLYPATDPRVLQKRKSIEETQKELSKQVVNI
                     VTSQQKIDNPIYSALMAELVAKEIDFQLSAATIEAIENGRKEYDRKMKGLPALEQELT
                     ELLREENINEQVYIAIYKSYIDAQIAEAGIVDQTYIVDSAVPPLQAAKPQKKLIVAIG
                     GVLGIFLGILFIFVLEYLEKRLMDIEELSKMTRGAVPIFGFFPKTKTGLLPIDPKRPD
                     RDLSEAAETAYTRAHLTEREGASKVIAITSPEPNSGSSTIAYYLAQTIARSRERVLLI
                     DGALRYPNLTKRLGLPNQSLGFSQAISSGFNERVAPLSLLPGLDFLPAGKTEEHPLAL
                     FRGKRFEEWIAATAKEYDRILIDTPAMSEDVDASMIIKRTAGVFLVVHLGKTEKRAFE
                     KALNDIAYAQVPLLGLLVNKYQSPKGFFFSGKERRLARNA"
     gene            7809..9332
                     /locus_tag="PKM49_05730"
     CDS             7809..9332
                     /locus_tag="PKM49_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY82349.1"
                     /translation="MKAIILDGYVDEPALFGVPPFLSTYVRYAWGAYRHFGVEAEYRT
                     IDQVRRENSWETLDSADHLVIIGGVSVPGKYAGGAPLSYSELTKIAAQCPNPLKILFG
                     AFSYRDTDSATHQKTPSRPARASVKDPLKAIECVVTGNLETYLYGLLSGKNPPEDIGK
                     DPEITEICALAGAGVVDRHPNFPNLICELEVSTGCERRSHCSFCSEPVFHPRFQSRDP
                     EAILTEVAALGERGVKRFRLGRAANILAYGDRGEGPDPFALLRIYRGIRERVPDLRTL
                     HTDNTNPQYIADHPVASAKILETISEYNTPGDILSFGVESFDEGVIRSNNLGTRVESI
                     YEAIKLVNEIGGFRKEGIPALLPGINLIAGLIGETKRTFEKNFDALKMILEKGWLLRR
                     INLRQVMVFPKTPLSAYRQTHFLPFHTREFRRFKEKVRTEIDRPMLERVFPVGTVIPD
                     VITEYREGNNTFGRPLWTYGILIGIRSTLPIGKKVTISVTEYGFRSLTGELISVQEN"
BASE COUNT         2396 a         2536 c         2603 g         2134 t
ORIGIN      
        1 aactcgccga tgataatccg cccggggttg ttgcgttggg cttcctgtaa tcggacgatt
       61 ggatcgttga ggatgttctc ttcttcagtc tttccctctt cggtcacctt acgcttcggc
      121 agattgaggg ccttctcttt gaaatgaagc atcatttcgg gcgcgccccc gattccggca
      181 tagcggccca gataggtcgg acattgggtg atcacttcga tgaaagaaaa gcctgagttt
      241 tctaagccgc gcatgacgaa gttttgggta ggcaaaaagt ggtaagtcgt cgaccgcgca
      301 acgtaggagg cgcccgcagc gatggccata cgggaaaggt cgaaggcgtt ttcaaagttc
      361 ccgtaaggtg aggttgtggc atacgactcg cggggagtcg ttggggaata ctgtcccccc
      421 gtcataccat agatgttgtt attaaaacac accacggtca ggtctatgtt tcgacgggcc
      481 gcgtggataa aatgattccc gccgatcgcg gcgaggtctc cgtctcccgt gatgacgatc
      541 actttgatat ccgggcgggc cagcttcacc cccgtggcaa aagcgatcgc tcttccgtga
      601 agggtgtgta gcgtgttgaa gtgcaaatac cctgtcgctc gcgaagagca cccgattccg
      661 gataccacgg ccactttatt cggatctagt cgaagttcat ctatcgcgcc cacaatcgcc
      721 ttcaaagcga taccatttcc acaaccaggg caccaaacgt gaggcaagcg atccgtacgc
      781 aagtacttga ggtatttttc caaggacata ttttccctcc ggccacgatg atatttatta
      841 aaaattatac cacggaacga tgaagaaaac aaccagccac catttcgggg ctatttatga
      901 taaaataccc aggacaatcg atttcgagag caatgggagg aataggcgta tgacatacga
      961 tctgatcgtc gtcggttcgg gccccgcggg ttacacgggt gcgatacggg ccagacaact
     1021 gggacttcgg accgccatca tcgaaatgga caaacccggc ggcgtctgtt tgaacgtcgg
     1081 atgcatcccg acgaaggccc tcatccataa ggccaaagag ttccgcgcgg tcgaaggact
     1141 taaagcgcac gggctccagg tggatgccag tcgatttgat ttttcagggg tgacccagta
     1201 cgctaaaatc gtgtcgacca aactttccaa aagcgtcgca ttcttgttga agaaagcggg
     1261 agtggatacc atcaacggaa gggccaaatt catatctccg acgacccttg aagtctctcg
     1321 gggagaggag agagaggtgt tgaccggccg gcattttttg atagcgacgg gttcgcgccc
     1381 gaaaaacctt ccggggcttg tgatagacga agagcgtatc ctctcttcca ccggcgcgtt
     1441 attcctgaaa cagcttccta aaaggatagc catcatcgga gccggagcga tcggcgtgga
     1501 atttgcctac atctaccgtt cgttcggatc ggaagtggat ctgatcgaag ttctaccgag
     1561 gatcctcccc ttggaagact cggaagctgt agatactctc acgaaacggt atatcaaatt
     1621 aggtatccaa gtgctcccct caacgcgggt gttggaggcg caacccatcg aagaaggggt
     1681 acggcttacc atacaaaagc aagatggggc gcaagagacg ttgacggtgg acaaggtatt
     1741 gctggcggtg gggagaacgc tcaatacgga tcaattgggt cttgaaaata tcccgttaac
     1801 gacggaaaga gggtttttac gcgttaactc gcgctatcaa accgccgttc cgcacatcta
     1861 cgcagcaggc gacgtgatcc ctacgccttt gttggcgcac gtggcctccg aagaggcgat
     1921 caacgccgtc gaatcgatcg tggacccgca gaaggaaccg atcgacttct ccggtcttat
     1981 accctcctgt atctattcgg aaccggaatt ggccagtttc ggagatcagg aggcacaact
     2041 cgtaagccaa aacaggccgt tcaagaagac cgtttttccc taccgaggga tcgggaaatc
     2101 agtcgcgacg gatcagccgg aagggttcat caagctgctc agcgatcccg agaccgaacg
     2161 ggtcctaggg gccactatcg tgggcgcgca ggctaccgaa ctgatacacg aatatatgct
     2221 gttgaagcgt acgggtcaac cgatccggat gctggcgcat atgatccacg cccacccgac
     2281 cttatccgaa gggatgatgg aggcggcgaa attggcccta ggtgaagcga ttaacctatg
     2341 aaaccgttcc actgcacctt ggtggtcttt ctgctcgcgg ccactctcgc ctgcgcttgg
     2401 acgaccgatt tgaaaaccat caagacccgt tacacgcaag aacagatcgt cgcccttttc
     2461 gaacaagctc gtcagtatga atggctggaa gagtatcagg agaaagacca cgccgtctac
     2521 atcctcacga gcaaatttct ataccgtgcg cggcttctcg acgacgacac cctcgtgttc
     2581 gaccagatta agagccggta ttattacgat atgcaggttc ctttttggac gatcatcctg
     2641 gtcttcctca tctccggggt tgctttgttt ttgattttct tcctaccctc cgactggcga
     2701 ttccgaaaga aaaaaccctg atgcgacacc aatgataccg gctgtcagaa ttgatttcaa
     2761 gccaaaaaag aggagagcct aatgaacaaa agacacctat ctggagtatg tctcttcatc
     2821 gccctgctca cgatctcttt ctgccagtac gtgctcaaac cgggggatca ggtcgaggtg
     2881 tacaactacg cttatcccga attgagccag gaagctttgg ttggcaagga cggttatatc
     2941 caactcaaac tgatcggagc ggtcaaagcg gaagggatga ccttggagga actggccgct
     3001 ttcgccaaaa acaagctgct cgcgtttgct cctgaatctc gggtgacggt cctattgaag
     3061 aacgtttatc cgataaaggc ctacatcatt ggagaagtgt atgcgtcgac cgcgatcgat
     3121 ttaacctacg aagacaatcc gacgctcctg aaggcgttga gtttttgtgg gggcgtgaag
     3181 cccaccgctg acttgaagaa catcctcctt cggcatgacg atggcacatt cgatacgata
     3241 gacatctccg cgaccctgga gtacgggcgc gacgtcgcgc cgacgtcaat tcgctcggga
     3301 gacgtcatcg tggttccgag aaagtacgaa cgacgtgtct acgtgtttgg gagcttggaa
     3361 caatcgggcg tcgtctactt cgaggaaaaa gagccgatgg tactcgaggc tctgctttcc
     3421 aagatcaaat acgatttagg cacttgcgag ccgacgatcg ccctcattcg cgacggacac
     3481 gaaacgactt tgagactcga ggcggccttt cctcaagaaa ggaaaaccgc gctcgaaagc
     3541 aacgacatcg tcttggtcag gaccctacag ccaaggtatc tatatgtgac aggtttagag
     3601 agcgcgaacc gcgtcgattt ccaaagagaa gaagagatga cgctgcagac cctgttaggg
     3661 aaactgggga tcgggccggt ctctctactg tccttacgca ttattccgtt gaaaggggcc
     3721 gagaagacgg tcagaatcga tcgtaccgtt ccttattacc ttttcgagac aggggatatc
     3781 gtcgagtttc ctccacagaa ctacgtgtac gtcagtggga acgttggcgt cacgggaaag
     3841 atcgactttc cctactctga gtcgttcact gtggaaaggc tgatggccat aatcggtgta
     3901 ggggaaaagg aaaagggcct gttgacgatc atcaatcccg acggtcagcg cttcttatac
     3961 cggtacccgg agcagatcag tagcagaaca ccgcttgaaa ggggagctta cgttcagttc
     4021 ccgccacgac gctacgtgtt tttggccgga gcagtttcag aaaacgtcga acgacaactg
     4081 tattttccaa acaacgaaga gatgacttta aaacacgcct tggccaaagc ctctgtcgat
     4141 ctgtccttat acagctactc cattacggtc atccgaaatc aagaacagta ccttttcgat
     4201 ctgaagagcg ccttgctctc ggaaggcgaa ttctttttgg agaccggtga tattctctat
     4261 tttgaaaaag acacggaccg gtacgtgcat ctggtcgttt cagacggcga aagtcgaaag
     4321 tattcgttta ggcctgagga tcctttggac cttttcactc tcctcataaa atacgaaaag
     4381 acgggggcgt taaccggcga tcagattaag atcatcgacc cgtccggacg tgtttttctc
     4441 agttctgtca aatccgtttt gaatcacgag gagaactatg agctttcacc ggggagtctc
     4501 gtcgtgatca acgccaacga gcggagggtt ttcgtattgg ggaacgcgca ggaaggcgga
     4561 gagattctct ttggccgcga tgagcccttt accctgacga cgcttatcgc caaatgccac
     4621 cccgttttgg atgaaaggac gcagagcgta atcattcagc aaggcgggaa agtgacggtt
     4681 ttggatatac gggaagcgtt ggatgttacg gccaccttcc ccttgcaaaa agacgcagtg
     4741 atctatttta aaccctatga gccgaggatc gtgacggcct tcgggcaggt aagaaatccg
     4801 ggtctgctat cttttggagc gcgtgagccc gtaaatctga tgacggttct ttcgaaggct
     4861 ggaggactgc tatccgatgc cggccaacgg gtgaaagtga tcggcgagga tggaaaaatc
     4921 gtcacgcaca acctactgac cgaacccaac ctcgaaaggc tctcgatcga ttcgggcagc
     4981 tacgtcgtgg ttgagagtaa tcttgaccag cacatcgcca ttttagggga tgtgaggacc
     5041 ccggggctgt actatcttcg tgacgagaag gtttctttgc tcgaattgtt aagtcaacgg
     5101 ggtggtgtca gcgactggac gctgaacacg acgttggagt tgacccgcgc ctccggagaa
     5161 aaatccttga tcaacatcga cctcgatcct tctgccttaa gcacgacgat gttgagagcg
     5221 ggcgacaccg tcttcgtttt cccgagcagt cgcttgaaag tctatgtctt cggggcggtg
     5281 gtgaatccgg gaatcgttcc ctaccggtcg aagatgaccc tgctcgaagc cctgctatac
     5341 tgtcgtgggc cctcagcttc cgcctacatg aaaaagctga tgattttccc cggcggtgtg
     5401 gacgcgaacc ctgtcctgtt agattactca gggttcagaa aagagcaagg tgtcgtggat
     5461 tattttctgg agccgggaga tgtcatttac gtaccgcaga gcgcattggt agagatcaaa
     5521 gacattgcgg cttttctggg gacaatgttg acgctcgtca attctggctt caccttaatg
     5581 gatagattgc aaagcttgcc ttaaagatta tcacaaatgg gaggggtctt gtgtcttcgt
     5641 tcgaaagaga agagcttatc gacaacgagt tgaatgagct caccctaaac gacctgttcc
     5701 gaatgttcag gaggagactt gggtggttct ttcttattgt cataatcaca attgtcgcca
     5761 cggccttcta cgccttcctc tctccaaaag tctacgaggc cagtgtcacc attaagctga
     5821 ctgctgcgac gtccgccggc gtttccagcc tccttcaggg tttgcctttc ggaataggtg
     5881 gcgtcgtcag cgggggaagc gtgactacgg agattgaatt gatcagtagt agaagcaaca
     5941 tagagcaagt gatccgcaat ttaaatctct tagatatcct gattccgcct gaaaaaagga
     6001 aaccccagga atcggagcag gctctgatgg tccgcgccat cgacgcttta tcagatcaga
     6061 tcagcgtgaa cccggtgaag aacaccaaca tcgtgaagat caccgtgtct ttttccgaac
     6121 cggaactcgc cgcgcgcatc gccaacgaac tggccaaggt ctacaacgca aacctttacg
     6181 atatcagcaa aacccaggcg agggaaaaac tgaagttcat agaaactcag ctacccatca
     6241 gcaaagccgc tcttgacctc tccatccaga aggtgagaga gtacaaagag gccaagcaga
     6301 tatttctgat atccgaagag gcgaaagcgc tattggatgt tatcgtggat ttcgacaagc
     6361 ggttgaacgc gaaaaaaatg gaattgcaga cgacacaggc cggaatcgcc gtgatacgcg
     6421 aaaaactctc cgcgactaac gaaaaactga tctcctccga aacgatctcg atcaatccgc
     6481 tcgtcagtga actgcgtagc aaactcacta atttgaacat cgaattagcc gcgctggaga
     6541 agctctatcc agcgaccgac ccgagggtgt tgcagaaaag aaagtcgatc gaagaaaccc
     6601 agaaagaact gtcgaagcag gttgtgaata tcgtcacctc ccaacaaaaa atcgataacc
     6661 ccatctattc ggcgctgatg gccgagctcg tcgctaaaga aatcgacttt cagctatcgg
     6721 ccgcgacgat agaggcgata gagaacgggc ggaaagaata cgaccgcaag atgaaaggcc
     6781 tccccgcact cgagcaggaa ctcaccgagc tccttaggga ggagaatatc aacgaacaag
     6841 tgtatatcgc catctataag agctatatcg acgcgcagat cgccgaagcc gggatcgtcg
     6901 atcaaaccta catcgtggat tccgcggtcc ccccattgca ggccgcgaaa cctcaaaaga
     6961 agctgatcgt cgctatcggc ggggttttgg ggatattttt gggcatcctc ttcatcttcg
     7021 ttctcgaata cctcgaaaaa cggctgatgg atatcgaaga gctttcgaaa atgaccagag
     7081 gggcggtccc gatcttcgga tttttcccga aaaccaagac cggcctcctt ccgatcgatc
     7141 cgaaacgtcc ggatcgcgac ctgtctgaag cggcggagac cgcgtacacg agggcccacc
     7201 tcaccgaacg cgaaggggca tcgaaagtga tcgccatcac gagcccggag ccgaattcgg
     7261 gaagttccac catcgcctac tatctcgcgc aaacgatcgc ccgaagccga gaacgggtcc
     7321 ttctgataga cggggcgttg cgctatccga acctgacgaa gcggctcggc ctcccaaatc
     7381 aatccctcgg cttctcacag gccatttcgt cggggtttaa cgaacgggtt gccccgctgt
     7441 ctctcttgcc gggtctggac ttcttgcctg caggcaaaac ggaggagcac ccgttggcct
     7501 tgttccgggg aaaacggttc gaagaatgga tcgccgccac agcgaaagaa tacgaccgga
     7561 tactgatcga taccccggcg atgagtgagg atgtggacgc ttcgatgatt atcaagagaa
     7621 cggccggtgt cttcctcgtg gtccatctgg gaaagactga gaagcgcgcc ttcgaaaagg
     7681 ccctgaacga tatcgcctac gcgcaagtcc ctctgctagg ccttctcgtc aacaaatatc
     7741 agagcccgaa aggcttcttc ttctccggga aagaaaggcg cctcgcgaga aacgcttagc
     7801 gaactcggat gaaggcgatc atccttgacg gttacgtgga cgaacccgcg ctcttcgggg
     7861 tccccccttt cctttctacc tacgttcgtt acgcttgggg cgcctaccgc cattttgggg
     7921 tagaggccga gtaccgtacg atcgatcagg tccggcgcga aaattcctgg gaaacgctcg
     7981 attcggcgga tcatctggtg atcatcggag gggtgagcgt cccggggaag tacgccggag
     8041 gcgcaccgct ctcctattct gaactcacga agatcgcggc ccaatgcccc aatccactga
     8101 agatcctctt cggggccttc tcttatcgcg acacagatag cgccacacat caaaagacac
     8161 cctcgagacc cgcacgagca tcggtgaaag accctttgaa agcgatcgaa tgcgtcgtga
     8221 cagggaacct cgagacctat ctgtatgggc ttttatcggg taagaacccg ccggaggata
     8281 tcggaaagga cccggaaata acggagattt gcgctttagc gggcgctggg gtcgtcgatc
     8341 gacatccgaa ctttccaaac ctgatctgcg agctcgaagt gtccaccgga tgcgagcgcc
     8401 ggtcccattg cagtttttgt agcgaaccgg tctttcaccc acgtttccaa tcgcgtgacc
     8461 cggaggccat actgacggaa gtggctgccc tcggagaacg aggagtgaaa cgtttccgtt
     8521 tggggcgcgc cgccaatatc cttgcctacg gtgaccgagg agaggggccc gatccgttcg
     8581 ctctactccg catctatcga gggatacgag aacgggtgcc ggacctgcgc accctgcata
     8641 ccgacaacac gaacccccaa tacatcgccg accacccggt cgcgtccgcg aagatcctcg
     8701 aaacgatctc ggagtacaac actcctggcg acatcctatc attcggagtc gaatctttcg
     8761 atgagggcgt catccgttcg aacaacctcg gtacgcgggt ggaatctatc tacgaagcga
     8821 tcaaactggt caacgagatc gggggatttc ggaaagaagg cattccagcg ctcctaccgg
     8881 gtattaacct gatcgccggg ctgatcggag agacgaaacg cactttcgaa aaaaacttcg
     8941 acgcgttgaa aatgatcttg gaaaaagggt ggttgttgcg tcgcatcaat ctccgccagg
     9001 tgatggtctt cccgaagacg cccctttcag cttacaggca aacccatttt ctccctttcc
     9061 atacgaggga gttccggcgt ttcaaggaga aggtgcgtac ggaaattgac agaccgatgc
     9121 tcgagcgggt cttccccgtc ggaacggtca tacccgacgt catcacggag tatcgtgaag
     9181 ggaacaacac cttcggaagg cccttgtgga cctatggaat cttgatcggc atcaggtcca
     9241 cgctccctat tgggaaaaag gtaacgatct cggttaccga gtacggcttt cgctccctca
     9301 ccggcgaatt gatatccgtt caagagaact aaccgaagga ggaaagcgga tacgtgtggg
     9361 tcaagctttg gggtgtaatc aagaaacaag accagaatca atcgcacggt ttttagtccg
     9421 tgtggtcatc gcggcgtaag cgccccttct tttcgcgacg gcgcgatacg cttttgtata
     9481 tgtcttgaaa aatatggttc gcacggagga cacggagaac acagagaaaa gaatcttgtg
     9541 aacgtcttgt gaacgtcttt ggaacgcctc ggaacgccgt ctttcagttc ggcaggtctt
     9601 aaaagagaac ggcagcagag cgtatcgcga gacttcgtga gcttccgaag tcagcccgct
     9661 ggttacccc
//
LOCUS       DAOPXN010000078         9595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7159_length_9595_cov_4.887317, whole genome shotgun sequence.
ACCESSION   DAOPXN010000078 DAOPXN010000000
VERSION     DAOPXN010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7159_length_9595_cov_4.887317"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /gene="tgt"
                     /locus_tag="PKM49_05735"
     CDS             <1..755
                     /gene="tgt"
                     /locus_tag="PKM49_05735"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNY82350.1"
                     /translation="FFTPELTVEIQEALGSDIAMVFDECAPHDAPETYIRQAMKRTYE
                     WAKRFYKAHSKEDQMVFGIVQGGVSIPLRQESAEQITSIPFDGYAIGGLSVGEPESVT
                     LAALEATCAYLPKDKPRYLMGVGTPELIFASVALGVDMFDCVLPTRSARHATLYTSGG
                     KVHIKSAKYKRNREPLDPRCDCYTCRHFSRGYLQHLYARSEVSGMVLGSLHNIRFLTA
                     MMENIRISIRERRFEAYRKAFFDRYRNQDGSE"
     gene            792..2438
                     /gene="hutU"
                     /locus_tag="PKM49_05740"
     CDS             792..2438
                     /gene="hutU"
                     /locus_tag="PKM49_05740"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HNY82351.1"
                     /translation="MDRVIRSDRGNALSCGSWMTEAPMRMLMNNLDPEVAKDPAHLIV
                     YGGNGKAARNWACYDKIVETLKRLKEDETLLIQSGKPVAVFQTHEWAPRVLIANSLLV
                     PKWATIEYFRELEERGLIMYGQMTAGSWIYIGTQGILQGTYETFAACARKYFGGTLKG
                     KWVLTAGLGEMGGAQPLAVTMNEGVALVVEADKRAIDRRIEKEYVDMWTDDLDEAIRW
                     VKKALKEEKPLSIGLLGNVGDIYPELLRRDMIPDALTDQTAAHDPLNGYIPMGMTFEE
                     AVKLRTSDPQAYLERVYHTVLVHVRALLDFQKRGAKTFEYGNNLRRLAFDHGEEDAFN
                     IVGYMPEYIRPLFCEGNGPFRWAALSGKAEDIYKTDEKVLELFPDNAHLKKWITLARE
                     RIRFQGLPSRICWLNYGQREKMGLAINEMVKKGEISAPIAIGRDHHDTGSVASPYRET
                     EAMMDGSDAIADWPILNALMNTASGATWVSVHHGGGVGIGFSIHAGVVIVADGSALSH
                     KKIARVLNNDSGLGVVRHADAGYPIALKTAKDKGIDTPML"
     gene            2570..>2887
                     /locus_tag="PKM49_05745"
     CDS             2570..>2887
                     /locus_tag="PKM49_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82352.1"
                     /translation="MKRYAFLIPLMLIGFVSFAVTVSFWEIPFPEGTGPTAKGIGGIY
                     NNPAVLSLETNWQFGLMTSLWNSVGDEHYHIVGATLIQPYQYGFAGGLDILYGQLKDQ
                     AGSN"
     assembly_gap    2888..2897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2898..3440
                     /locus_tag="PKM49_05750"
     CDS             <2898..3440
                     /locus_tag="PKM49_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82353.1"
                     /translation="FGYAISGLWNRLSWGARIIAGYFDNDPESSVYGSANLGLMYSVL
                     DNTHILFNFNAPQIVTTKPLVFDINRPETFNIGAGILTANDIGRVYAGMTAYNKNENP
                     YFGFMFGGGIKMAFLNWDLAFVGKDLFVGIPIDEMNAVIKASLYLDFEYFAIGVGAQM
                     PLTTYGFSETILSAFVRAKW"
     gene            3549..4046
                     /locus_tag="PKM49_05755"
     CDS             3549..4046
                     /locus_tag="PKM49_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82354.1"
                     /translation="MRTVLIAVVFGFVFFACGCAFMGLGVITDISKPDGPNPEDATPT
                     SWYIRNVFQGIWNYQYLLSGTSVQVYRVTHEVQKNGELKFTFALPITGVKHFLATGTI
                     DMNTGLFFGGSAWAQIGNEEGAITFTGTFATQTSQIEAYSVILDRRFNPGELIFYTIS
                     ANRVQ"
     gene            4047..4757
                     /gene="ispD"
                     /locus_tag="PKM49_05760"
     CDS             4047..4757
                     /gene="ispD"
                     /locus_tag="PKM49_05760"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNY82355.1"
                     /translation="MGVTGSCVALLMAGGAGRRFEKALPKQFFLLSGKPLIRYALETF
                     QRSPQIDGIFVVYERTTEAQTRSIAESYSKTVGLVAGGPTRQDSVYLGLEALSQIGQP
                     SRVLIHDCVRPFFQTVLESVVSELKGHEAVIPALDIADTLYHYDGERILSLPKREEYY
                     RVQTPQGFSFSGILAAHRKAQEENRRDFSDDGSLYAFFGGALRLVNGSPLNIKITTPM
                     DIPIAMVYLRYIENKGVV"
     gene            4759..6066
                     /locus_tag="PKM49_05765"
     CDS             4759..6066
                     /locus_tag="PKM49_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNY82356.1"
                     /translation="MVKNYVLDTNVLLHDPHALFSFQDNHVYIPMPVLEELDRFKKSP
                     GALGANCREVIRKLDGLRAFGDLLKGVRTDAGGLICVKIFQDKEFENVVLPSYFKKGL
                     ADNWILLYLMKIQKIDPKMRTIFVTKDINFRLKSQIIGVTAQDYLTDKSAVTEDRGFY
                     EVSIPEDAMRTFDEEGRISFSALSLPSSMKKRNVFFDFGRSLYGKTTETTDEIVRLEV
                     GLGTSVYGITARNREQLFALEVLRDDSIPLVCLTGKAGTGKTLIAIAAGLSKVMEENA
                     YSKLTVTKPVVSMGQDIGFLPGTAEEKIRPWVQPVYDNLDFLFARKGVRAEEYLKKRK
                     IIEVEILSYIRGRSIPHQYIIIDEAQNLTPHEVKTIITRAGEKTKIVLTGDPDQIDNP
                     YLDRTSNGLMVLASKFFDHPLAAHITLEKGERSALATVASEIL"
     gene            6123..6656
                     /locus_tag="PKM49_05770"
     CDS             6123..6656
                     /locus_tag="PKM49_05770"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNY82357.1"
                     /translation="MNGEYDQYLSTFIDEAQEYMRVLNDKMLALESEPENMTAINEIF
                     RAIHTLKGMSGTMGFETMTKLCHRMENVFDRARNGEIEVDKDMMDALFSGVDHLERMT
                     QSIASKGSDSGTDITEILAVYERINRGEKKPPEKKPAPKPPTVVEKKASKSPSKQPKT
                     AGIPAETGIKVKGRRSD"
     gene            6662..8341
                     /locus_tag="PKM49_05775"
     CDS             6662..8341
                     /locus_tag="PKM49_05775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNY82358.1"
                     /translation="MKQPEPQVQGTQKPDYSEGFESPIPLPSEIQNTLRKGIEKGYVP
                     YYIQVKLTSGTLLKGARIYMVFHRMDEMNSEILYSVPDVETIEDERFDLTVEVVLLSK
                     VPQEKIAEAILNIAEIETVKIHPLRFFEDIEPESTPPPAQVVGQKPIESPRMGQADLE
                     QKAPPVEHTVAPSGAASLSKTIRVDIEKLDTLMNLMAELVIARSRINETLKKYNIKQV
                     AESLSQLSRITLDLQNIVMKIRMVPVAYVFNRFPKMVSELSKSFNKEVQLLISGQETE
                     LDRTVVDEIGEPLVHLLRNVIDHAIETKEERLKKGKPAAGVARLNARHEGNTVIIEVI
                     DDGKGLDRDSIARKAIEKGILDEPTAARMSDEEIFLLIFLPNFSTKKQVTELSGRGVG
                     MDVVKTRIESLNGSVSLESVKEVGTKVKIRLPLTLAIIQALLVNVSENVYAIPISMID
                     STLNLPIADIQLVQSREVVVIRGEIIPIIWLQEVFGLSRNRERQSVHVVITRVGQKKY
                     GFVVDHLLGQDDIVIKSLGRLFKNVKEFSGGAILGDGRIALILEGTTIVAT"
     gene            8383..8748
                     /locus_tag="PKM49_05780"
     CDS             8383..8748
                     /locus_tag="PKM49_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019532545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY82359.1"
                     /translation="MAKRVLIVDDAAFMRMILKDIMVKNGFEVAGEASNGLEAIEKYR
                     ELQPDVATMDITMPELNGIEAIKRIREQYPNAKIIVCSAMGQQAMVIDAIQAGAKDFI
                     VKPFQHSRVVEAIHKVTAG"
     gene            8750..9157
                     /locus_tag="PKM49_05785"
     CDS             8750..9157
                     /locus_tag="PKM49_05785"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNY82360.1"
                     /translation="MPTEVVATKTTAGSFLVSAFLPFIFATLLLFVVLAVVLWLTKHT
                     GKARAKGDFFFILASQPVDRFSNIHLIRFLNEFYVVVTTNGNVCILQRIEEPESKEHL
                     NLRYTHRKVSPFLSAFNKKVFEEELKKMDHLSE"
     gene            9165..>9595
                     /locus_tag="PKM49_05790"
     CDS             9165..>9595
                     /locus_tag="PKM49_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HNY82361.1"
                     /translation="MKPASRVLLLFVCLILLVASTTGLTQDVRIPIPRIELGIGEAQN
                     PNDLAITLEILFLLTILTLAPSILLMFTSFTRLVIVLGVVRGAIGTRQTPPNQVIVAV
                     ALFLTFFTMQPTFNKVWNQAWVPYSENRITYQTMFERTGDI"
BASE COUNT         2442 a         2285 c         2657 g         2201 t
ORIGIN      
        1 atttcttcac acccgaattg acggttgaga tacaagaagc tttgggttcc gacattgcga
       61 tggttttcga cgagtgcgcc ccacatgacg cgcccgagac ctatatccgc caagcgatga
      121 aaagaaccta tgaatgggcc aaacgcttct ataaagctca ttccaaagaa gatcagatgg
      181 ttttcgggat cgttcaaggg ggcgtttcca tccccttgcg gcaggaaagc gccgagcaga
      241 tcacctcgat cccgttcgat gggtatgcaa tcggcggatt gagcgtcgga gaacccgaat
      301 cggtgaccct cgcggcgctc gaagcgacgt gcgcctatct accgaaggat aaaccccgtt
      361 atctgatggg ggtagggaca ccggaactca tcttcgcgag cgtcgcctta ggcgtagata
      421 tgttcgattg cgttttgccc acaaggagcg cccgtcacgc cacactctac acctcaggcg
      481 ggaaggtgca tatcaaatcc gcaaagtaca aacgcaatcg agagccttta gacccgcggt
      541 gcgattgtta cacctgtcgc catttttcca gagggtacct ccaacacctc tacgcgcgct
      601 cagaagtctc ggggatggtt ttaggcagtt tacacaatat tcgcttttta accgcaatga
      661 tggagaatat tcggatttcg atacgggagc gtcggttcga agcctatcga aaagcatttt
      721 tcgatcgata tagaaatcaa gacggctccg aataacccga tatcttccat cgacaatgaa
      781 ggaggacgtt tatggataga gtgatccgat ccgaccgcgg caacgcctta tcctgtggga
      841 gctggatgac ggaagcgccg atgcggatgc tgatgaacaa cctggatccg gaagtggcga
      901 aagaccccgc ccacctcatc gtctacggag ggaacgggaa ggctgctcgg aattgggcgt
      961 gttatgacaa gatcgtcgag acgctgaaac gtttgaagga agacgaaacg ctcctgattc
     1021 agtctgggaa accggtcgca gttttccaaa cacacgaatg ggcgccacgg gtgctcatcg
     1081 cgaactccct gttggtgccg aaatgggcga cgatcgagta cttcagggaa ttagaggaac
     1141 gcgggctcat catgtacggt caaatgaccg ccggcagctg gatctatatc gggacgcaag
     1201 ggattttaca ggggacttac gaaaccttcg ccgcttgtgc gcgcaagtat ttcggtggga
     1261 cattgaaagg gaaatgggtg ttgaccgccg gattgggcga gatgggaggc gcgcagccct
     1321 tggccgtaac gatgaacgag ggtgtcgctt tggtggtcga agccgacaag agagcgatcg
     1381 ataggagaat agaaaaagaa tacgtggata tgtggaccga tgatctcgat gaagcgatcc
     1441 ggtgggttaa aaaagcgctg aaagaggaaa agccgctttc catcggcttg ttgggcaacg
     1501 tcggcgatat ctatccggag ctgctcagaa gggatatgat ccccgacgcg ttaaccgacc
     1561 aaacggccgc tcacgacccc ctcaacggct atattcctat gggaatgacc tttgaggagg
     1621 ccgtcaaact cagaacgagc gatcctcagg cctatcttga acgggtttac cataccgttc
     1681 tggtacacgt tcgcgctcta cttgattttc agaagcgggg agcaaaaaca ttcgaatacg
     1741 gtaacaacct ccgtaggcta gccttcgatc acggagagga ggatgctttc aacatcgtcg
     1801 gctatatgcc cgaatacatc cggcccttgt tttgcgaagg caacggacct ttccgatggg
     1861 ccgcgctctc cggaaaagca gaggatattt ataagaccga cgaaaaagtg ttggagctct
     1921 ttcccgataa cgctcatctg aaaaaatgga tcaccttggc ccgagaacgc atccgctttc
     1981 agggattgcc ttctcgcatc tgctggctaa actacgggca acgcgaaaag atggggcttg
     2041 cgattaacga aatggtcaag aaaggagaga tatccgcgcc gatcgctatc ggacgcgacc
     2101 atcatgacac cggttcggtc gcttctccct accgagagac cgaggcgatg atggacgggt
     2161 cggacgcaat cgcggattgg ccgattctga acgcgctgat gaacaccgcg agcggagcga
     2221 cttgggtttc cgttcatcac ggtgggggcg tcggaatcgg gttttcgatc cacgccgggg
     2281 tcgtgatcgt cgcggatgga agcgccctaa gccataaaaa gatcgcgaga gtgctcaaca
     2341 atgactcagg tttaggggta gttcgacacg cggacgccgg ctatcccatc gcccttaaaa
     2401 cagcgaagga caagggtatc gacacgccga tgctctagtg caagtcttgg actttattct
     2461 ttgacatagc gttgaaggac ccggtgcgtc gctgtttgca gcgagatagt cgtcacgcga
     2521 tattcgattg tgggtatacc ttatgatacc gttccagggg gggattcgat tgaaaaggta
     2581 cgcgtttctt attcctttga tgttgatcgg gtttgtgagt tttgcggtta cggtttcatt
     2641 ttgggagatt ccgtttccag aagggacggg accgaccgcc aaggggatcg gggggatata
     2701 caacaaccct gccgtgctga gtttggaaac taattggcag ttcggtttaa tgacgagttt
     2761 gtggaacagt gtgggggacg agcattatca cattgtcggc gccacgttga tacaacccta
     2821 tcagtatggc tttgcaggcg gtttggatat cctttacggt cagctaaaag accaagcggg
     2881 ttcgaacnnn nnnnnnnttt ggctatgcca tttccgggct atggaatcgg ttatcttggg
     2941 gggcccggat tatcgccggg tattttgaca acgaccccga aagctcggtg tacgggtccg
     3001 cgaaccttgg gctgatgtat tcggttctgg acaacacgca catcctgttc aactttaacg
     3061 cgccgcagat cgtaacgacg aagcctttgg ttttcgatat caatagaccc gaaacgttca
     3121 acatcggcgc tggaatactc acagcgaacg acatagggcg tgtttatgcc gggatgacgg
     3181 cctacaacaa gaatgagaat ccctacttcg gttttatgtt cggcgggggc atcaaaatgg
     3241 ccttcttgaa ctgggacctg gcctttgtcg gcaaggactt gttcgtcggt ataccgatcg
     3301 atgagatgaa cgcggtcatc aaagcctctc tctatctcga tttcgagtac ttcgcgatag
     3361 gggttggtgc acagatgccc ttgacgactt acgggttctc ggaaacgatc ttgagcgcct
     3421 tcgtccgcgc gaagtggtga gatcttccag caggggtcta tctgtcgagc gcggttgaaa
     3481 gagcgagccg aggttctaat cttgactcga gactggcgcg tatgtgtaga ggagggacga
     3541 tcataagaat gagaacggta ttgattgcgg tggttttcgg cttcgtgttt ttcgcctgtg
     3601 gctgcgcctt tatgggactc ggcgtaatta cggatattag caagcccgac gggccaaatc
     3661 cagaggacgc gacaccgact tcttggtaca tcagaaatgt ctttcagggg atctggaact
     3721 atcagtattt gctttctggc acctccgtcc aggtttatcg cgtgacccat gaagtgcaga
     3781 aaaacgggga gctcaagttc accttcgcgc ttcccattac aggtgtgaag cattttttag
     3841 ccacgggcac catcgatatg aacacgggcc tgttctttgg cggaagcgct tgggcgcaaa
     3901 taggaaatga agagggtgcg atcaccttca cgggtacctt cgctacgcaa acctcgcaaa
     3961 ttgaagcgta ttcggtcata ttggaccggc ggtttaatcc cggggagctc atcttctata
     4021 cgatttcggc gaatcgggtg cagtaaatgg gggtaacagg ctcctgcgtt gcccttttga
     4081 tggcgggagg cgccggccga cgtttcgaga aagccctgcc caaacaattc tttttattgt
     4141 ccggaaagcc cttgattcga tacgcgctag agaccttcca aaggtcccca cagatcgacg
     4201 ggatttttgt cgtgtacgaa cggaccacgg aagcgcagac gcgttcgatc gccgaaagct
     4261 acagtaaaac cgtcgggctt gtggccggcg ggccaacgcg tcaagattcg gtctatctgg
     4321 gtctggaagc gctatctcaa atcgggcagc ccagccgagt tttgatccac gactgcgttc
     4381 gcccgttttt tcaaacggtt ttagagagtg tcgtttcgga gttgaagggc catgaggccg
     4441 tcataccggc gctagacatc gcggacacgc tctatcatta cgatggcgaa agaatcctgt
     4501 ctctccccaa gcgggaggag tattaccgtg tccaaactcc tcaggggttt tctttttccg
     4561 gcatcctcgc ggcccatcga aaggcccaag aagaaaacag gcgtgatttt tctgatgacg
     4621 gatcgctcta cgcgtttttc ggcggggctt tgcgtttggt gaacggtagc cccttgaata
     4681 taaagatcac gaccccgatg gatattccta ttgcgatggt atacctacgc tatatagaga
     4741 acaaaggagt ggtctgattt ggttaaaaac tacgttctgg atacgaacgt cttgttgcat
     4801 gatccgcacg ccctcttctc ttttcaagac aatcacgtgt acattccgat gccggtccta
     4861 gaggagttgg atcggttcaa aaagtctccg ggcgccttag gggctaactg ccgtgaggtg
     4921 atccgaaagc tcgatggtct tcgcgcgttc ggcgatctct tgaaaggtgt gcgcacggac
     4981 gcgggggggc tgatctgtgt taagatattt caagataaag aattcgagaa cgttgtctta
     5041 ccttcttact ttaagaaagg tctcgccgac aactggatac tattgtattt gatgaagata
     5101 cagaagatcg acccgaaaat gcgaacgatc ttcgtgacca aggacatcaa ctttcgactg
     5161 aagtcgcaaa tcatcggcgt cacggcgcag gactacctga cggataaatc cgccgtgacg
     5221 gaagatagag ggttctacga ggtttcaata ccggaagacg cgatgcgcac cttcgacgaa
     5281 gaggggagaa taagcttctc ggccttaagc ctcccgtcct cgatgaagaa gcggaatgtg
     5341 ttcttcgatt tcgggcggtc tctttacgga aaaacaacgg agacaaccga cgagatcgtc
     5401 cgtttggaag ttgggttagg gacatcggtt tacgggatta ccgcgaggaa ccgggaacaa
     5461 ctcttcgccc tggaagtatt acgggacgac tctatcccgt tggtttgttt aaccggaaag
     5521 gcgggaaccg gaaagacgct catcgccata gcggcgggat tgtcgaaggt gatggaggaa
     5581 aatgcctatt cgaagctgac cgtcacgaaa ccggtcgttt ctatgggaca agacatcgga
     5641 tttcttcctg gcaccgccga agaaaagatc cgcccttggg tacaaccggt ctacgacaac
     5701 ttagacttcc ttttcgcgcg gaaaggggtt cgagcagagg agtaccttaa gaagcgaaag
     5761 atcatcgaag tagaaatttt gagttacatc aggggccgct cgattccaca tcaatacatc
     5821 attatcgatg aagctcaaaa tttaacccca cacgaagtga agaccatcat tacccgcgcg
     5881 ggagaaaaaa cgaagatcgt cttgacgggc gatcccgatc aaatagacaa cccctatctt
     5941 gaccgtacga gcaacggttt gatggttctg gcgtcaaagt tcttcgatca cccgctcgca
     6001 gcccacatca ccctggaaaa aggagaacgc tcggcgttgg cgaccgtcgc ctcggagatt
     6061 ttataaacga aaccggattt atgatataat ttgaagccat tggtagagtc ggaggtgaaa
     6121 ggatgaacgg cgaatacgat cagtatttaa gcacgtttat cgacgaggcg caagaatata
     6181 tgcgcgtgtt gaatgataag atgctcgctc tagaaagcga accggagaat atgactgcga
     6241 tcaacgagat tttccgggcc atacatacgc ttaaagggat gtcgggcacg atgggttttg
     6301 aaacgatgac gaagctgtgt catcgtatgg aaaatgtctt cgatcgtgct cgaaatgggg
     6361 agatagaggt cgataaggat atgatggacg ccctattctc cggagtggat cacctggaac
     6421 ggatgacgca atcgatcgcc tcaaaaggct cggatagcgg taccgacatc acggagatcc
     6481 tcgccgtcta cgaacggatc aatagaggag aaaaaaagcc tccggagaag aagccggctc
     6541 cgaaacctcc gaccgttgta gaaaaaaagg cgtcgaaaag cccatcgaaa caaccgaaaa
     6601 cagcgggtat ccccgccgag accgggatca aggtcaaagg aaggaggagc gactagagcc
     6661 ggtgaagcag cccgaacccc aggttcaagg aacgcaaaaa ccggactatt cggaaggttt
     6721 cgagtctcct atccccttgc cttcggagat acaaaacact ctaaggaagg gtatcgaaaa
     6781 gggttacgtt ccctactaca tacaagttaa actcacctct ggcaccctgt tgaaaggcgc
     6841 gcgcatctat atggtctttc atcggatgga tgagatgaat tccgaaatcc tctactccgt
     6901 cccggatgtg gagacgatcg aagacgagcg ctttgatctg acggtcgaag tcgttctgtt
     6961 atcgaaagtc ccgcaagaaa agatcgcgga agccatcctg aacattgcag agatagaaac
     7021 ggtaaaaatc catcccttga ggttttttga ggatatagag ccggaatcga ctcctcctcc
     7081 cgcgcaagtc gtcggacaga aacctatcga gagcccgcgg atgggtcagg cggatctcga
     7141 gcaaaaagcg ccaccggttg agcatacggt cgcgcccagc ggcgccgcgt cgttgagtaa
     7201 aacgattcgg gtggacatcg aaaagctaga taccctaatg aatttgatgg ccgaactcgt
     7261 catcgcccga agccggatca acgagacttt gaaaaagtac aacattaaac aagtggcaga
     7321 aagcctcagt cagttgagtc gaatcaccct agatctccag aatatcgtta tgaaaatccg
     7381 tatggtgccg gtagcgtacg tcttcaaccg attccccaag atggtcagcg agctctcgaa
     7441 atctttcaat aaagaggtgc aattgcttat ttcggggcaa gaaacggagt tggaccggac
     7501 cgtcgtggat gagatcggag agccgctggt gcatctgctg agaaacgtga ttgaccacgc
     7561 tatagaaacg aaggaagaac gcttaaaaaa agggaagccc gcggctggtg tcgcgagatt
     7621 gaacgccagg cacgaaggaa acaccgtgat catcgaagtg atagacgacg gaaaaggctt
     7681 ggatcgagat tccatcgcga gaaaagcgat agaaaaaggg atcctcgatg agcccacggc
     7741 agccaggatg tcggatgaag aaatcttttt actcattttc ttgccgaatt tttcgaccaa
     7801 gaaacaggtg acggagctct ccgggcgcgg cgtcggaatg gatgtggtca agacccgaat
     7861 agaaagcctg aatgggtccg tctctttaga aagcgtcaaa gaagtcggga cgaaggtaaa
     7921 aatccgattg ccgctcaccc tcgccatcat ccaagccctg ttggttaacg tcagcgaaaa
     7981 tgtttacgcg atcccgatct ctatgatcga tagcaccttg aatcttccaa tcgccgacat
     8041 acaattggtg caatctcgag aagtggtcgt gattcgcggc gagatcattc caatcatatg
     8101 gctgcaggag gtctttggcc tttcgcgaaa tagagaaagg cagagcgtcc atgtggtgat
     8161 cacccgcgtc ggtcagaaga aatacggatt cgtggtcgat cacctgttag gccaggatga
     8221 catcgtcatc aaatccctcg ggcgtctgtt caagaacgtg aaagagttta gtggtggcgc
     8281 tattctgggg gacggaagga tcgcgctcat tctcgaagga acaacgatcg tcgctacata
     8341 aatttccggc ttactttttt gaatgtatag aggaggttag atatggctaa aagagtttta
     8401 atagttgatg acgcggcgtt catgcgtatg atattgaaag atatcatggt aaagaacggg
     8461 ttcgaggttg ctggtgaagc gagcaacggg ctagaagcga tagaaaaata ccgggaattg
     8521 caaccggatg tagcgacgat ggatatcacg atgcccgaat tgaacgggat cgaagcgata
     8581 aaaaggatca gggagcaata cccgaatgcg aagatcatcg tctgtagcgc gatgggacaa
     8641 caggcgatgg tgatcgatgc gattcaagcc ggagcgaagg actttatcgt gaaacctttc
     8701 cagcattcga gggttgtcga agcgatccac aaagtcaccg ccgggtaata tgcccaccga
     8761 ggtagtggcg acaaagacaa cggcaggtag cttccttgtt tcggctttcc tgccttttat
     8821 ttttgcgaca ctgttgcttt tcgtcgtact tgcggtcgtt ttatggctga ccaaacatac
     8881 gggaaaggcg cgggcgaaag gtgacttttt ttttattctc gcgtcacagc ccgttgaccg
     8941 attttccaac attcatttga tccgcttctt gaacgagttt tatgtcgtcg tcacgacgaa
     9001 cgggaatgtt tgcattctac agcgtatcga agagccggaa tccaaagagc atctgaacct
     9061 gaggtacact cacaggaaag tctcgccttt cttaagcgcg ttcaacaaaa aagtcttcga
     9121 agaagaattg aaaaagatgg atcatctctc ggaatgaagg ttctatgaag cctgcctcgc
     9181 gtgttctcct tctttttgtc tgcctgatct tgctagtcgc gtcgacgacc gggttgactc
     9241 aggatgtgcg tataccgatt ccgaggatag agctcggaat cggcgaggcg caaaatccga
     9301 atgatttggc gatcacactt gagatcctct tcctcttaac catcctcacg ctggctccgt
     9361 ccattctcct gatgttcacc tctttcacac ggttggtgat cgtgttgggg gttgtgcgcg
     9421 gggcgatcgg aacgaggcaa actcctccga accaggtcat cgtggccgta gcgctttttt
     9481 taacgttttt tacgatgcag ccgaccttta acaaggtttg gaatcaagca tgggtgccgt
     9541 attccgaaaa ccgaatcacc tatcaaacga tgttcgaacg gacgggtgat atctt
//
LOCUS       DAOPXN010000079         9427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7318_length_9427_cov_5.701131, whole genome shotgun sequence.
ACCESSION   DAOPXN010000079 DAOPXN010000000
VERSION     DAOPXN010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9427
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7318_length_9427_cov_5.701131"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PKM49_05795"
     CDS             <1..526
                     /locus_tag="PKM49_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82362.1"
                     /translation="AEQTNLLALNAAIEAARAGEAGRGFAVVADEIRKLAEESKKATS
                     NIGTILKEVQKDSGESYQATEKTVELVKAVNEKARLVEQQFKQLLVMVDHTTGMIENL
                     TATSEEQGAAAEEMASAMDTSAKAAHEIAEQIRQMTEAIDQQSQSAQQITAAAQELDT
                     LSETLDALVKRFKI"
     gene            1462..3462
                     /locus_tag="PKM49_05800"
     CDS             1462..3462
                     /locus_tag="PKM49_05800"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82363.1"
                     /translation="MMKLKSLRAKLLLYFIPVSVSVLVISGFIIALSVRTGILEQTME
                     STEETLHTSVRIVEEMLAGIKTELRNLANTDVVKSFDPTRFVPRFVETIKQGEGLYET
                     FFLADPTGKAIMETGEAVDIRDRPYFQEVMFQGKKFAINDGMISRFAHIPAFGVAYPV
                     YDYTGQLIGVLGANVALKTLSEKVCATKIGQTGYIFLVDGSGTVIAHPDTDLILNLNL
                     LEGTKSKYKGLEEAGREMVSGREGFGNIVLPSGEKQILHYDSIPNTPNWTVAGVISEA
                     EVTQKSAYFLVTVVVIFAVIVGVIVLISMFVGSFISKSLKRLSAQVDQFGKGDLTTVF
                     EVKGRDEIAQIAQALSEMGNDLREAMVSIGEASQEIKAASTDLAAISEEQLAGSEELS
                     SQSASVNSNLQNTVAAIEEVNSGVDEVAASAQNVSKTAQALSTENNKTADGSREGGQM
                     ISDVVRQIEATTQQTLLTAERVQKLAENSKSVGEIVDTISSIAEQTNLLALNAAIEAA
                     RAGEAGRGFAVVADEIRKLAEESKRATSNIGAILKEIKKDAEESYQATDKTVSLVKDV
                     NTKSQAVEKQFKHLLDMVEKTTGMIENLTGTSEEQGAAAEEMASAMDTSAKAAHEISE
                     QIRQMAQGIEQQTQGAHQISSDAEELDSLAENLDTQIKRFKV"
     gene            3822..4196
                     /locus_tag="PKM49_05805"
     CDS             3822..4196
                     /locus_tag="PKM49_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNY82364.1"
                     /translation="MSGLEGNCEVHNPHDELCDRIAEKLPEESQIARAAELFKVFGDP
                     TRLKILTLLSISECCVCDIAKLIDLSQSAVSHQLRILRQTRLVKYRKQGKEAFYSLDD
                     EHVSEILSLGFEHAGEEQGGGR"
     gene            4193..6280
                     /locus_tag="PKM49_05810"
     CDS             4193..6280
                     /locus_tag="PKM49_05810"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNY82365.1"
                     /translation="MTQSFSVEGLTCAHCATKIERKVREAPDVLRAELNFVARTLTVE
                     TRGEELPANLQGIIQSVEPEARILKRSELPETPLLERLEDERRAMRIDLARLVAGSVL
                     FLVSILTSFSSRYALGLSLIAYGVIGYPVLLASWKKAMKRNLFNENFLMTLATICAFL
                     IGQPAEAVGVMIFYEIGELLNAYALNRSRYRIFGLLTHEPETVTAIKEGNATVVHPRE
                     VLPGESFIVKPGERVLLDGEIEKGEALIDTSSLTGEPVPRTFGKGDEVLSGFINTNAT
                     LRIRATKRYEDSTLSRTLSLIEEAALKKSRTERSISRFASVYTPAVVFGALALATLPP
                     ILFGLPFESWLYRALVLLVISCPCALVLSVPLASFVAIGKLSKQGILVKGSDFFRTLK
                     KAKSVVFDKTGTLTAGLLKIDRVTAFLPHTQEQVIHYAAIAEYHSSHPIARALRDGLP
                     EGFSPDEITQYQEYAGSGLSLNYRGTEILVGSARFLESRDIRIKGQADAPEALTTVLV
                     AVDGVHAGTVYFSDSERPDSAELIAFLRDRGLKTFLLTGDRAPAAKEVAQRLGLDGYF
                     SELSPKLKIEEYEKIRDQTGQSLFIGDGVNDAPVLRRADVGVAFGGKNSDITLESADI
                     VIPGEEIKKVKTLFESADRHAKILWQNILLILGIKGLFLLLGGFGLSSLWEAVFADVG
                     VTLLCVLNTFRLS"
     gene            complement(6336..8717)
                     /locus_tag="PKM49_05815"
     CDS             complement(6336..8717)
                     /locus_tag="PKM49_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNY82366.1"
                     /translation="MKCRLCFAVVFFTLLCLGMGFSMKTVFIVQSYDPDFIWSQQLDQ
                     GVRDALCGSDTQIQAYYLNARLKNQPDWKIKAGELVKTRIEEEKPDLLILCDDDAQEY
                     LGKEMRGVPIVFCGVNQFPERYGYPRENVTGVLEIPFFEESIQFARTFFPPVRRVLFL
                     GDQGITTEGILEHAKKAVLSGVETLETISVSTFEEWKTVILQSQTRYDAIFLAAYRVL
                     TDQAGHTVPTEEVGGWTARNSPVPTFALLDYMVEEGILGGVVQSPYLQGYEAGRLACE
                     ILLHQTAPTSLPLRALERGERMINVQRAREVGLEEPLFSAVSFDRIVGYDKIPERYYL
                     RSIVSLFEETIKGAENSLSTLANLPAPLRSQWEIVKPSLQVIEQRYPGVGIYGIPEGY
                     YTVTRNWTGLNLKDRDYYQLLEKGQTVKGASVYSRSTGEPSLVIGIPVMEGSTLTSYF
                     GLSLYLNPLVIRLRGQIPLPEGCAYYFFDWTGKLVFSNLEAIAYPEEHEMGALLAQMP
                     KSSQPEGSFFFTSPKGTYVAFYRKSLETGWTAFLTVKSGELESTADSPESQLESIQKR
                     TAASIARLTEGLKEGAERLTYSFANESLTRSILTELRTLDPAIVDVSFVNPDGIMKWV
                     EPEEYRNHEGADISGQDQVIRLRETKRPVISDIFMAVEGFLAMDIQWPVFDEFGGYAG
                     SISLLIRPERFFSILADAGQHTDSDFWIMHPTGTIVYDPDTWEIGRNLFTDPVYEPFE
                     ELRTLGRQMTVKSSGNGAYSFFAVGSDHVVQKKALWRTVRAFDTELKLIITYY"
     gene            8814..9299
                     /locus_tag="PKM49_05820"
     CDS             8814..9299
                     /locus_tag="PKM49_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82367.1"
                     /translation="MMKKRKITGFILLLCTLSVFSYTLLEQRDWLFDGDFILLEGQLV
                     LGKPIEISLLTLYPVFLIHRLDYRFNSSDESETAPRLLGMEMTPYALIVIGDKEQHIR
                     LISASEGATDTEAVNYAELMALSETIRKELYRKSPAENDPLNDFELLMIHVFAIKNVL
                     P"
BASE COUNT         2390 a         2383 c         2516 g         2138 t
ORIGIN      
        1 cgcggaacag acaaacctgc tggcgctcaa cgcggcaata gaggcggccc gggcgggaga
       61 agctgggcga ggcttcgcgg tagtcgcgga cgagatccgg aaattggccg aagagagtaa
      121 aaaggcgaca tcgaacatcg ggacgatact gaaagaggtg cagaaggact ccggtgaatc
      181 ctatcaggcg acagaaaaaa cggtggaatt ggtcaaggcc gttaatgaaa aggctcgttt
      241 ggtagagcaa caattcaaac aactcttggt gatggtcgac cacacaacgg ggatgatcga
      301 gaacctgact gcgacgtcag aggagcaagg ggccgcggca gaagagatgg cctcagcgat
      361 ggacacgtct gcgaaagcgg cgcacgagat tgcagaacag attcgacaga tgaccgaggc
      421 gatcgatcaa caatcgcagt cggcgcaaca gatcaccgca gccgcccaag aactcgatac
      481 cctctccgaa accctcgacg ccctggtgaa gcggttcaaa atatagattt cgatagacaa
      541 gtgaaacgcc ggcctttggc cggcgttttt cttcttggag cgctctggga acgctctggg
      601 attggcccgg gaacgccctg ggaacgccga actttagttc ggcatggttt ccgtttttca
      661 aagaaaaaaa cataacccat gccacactta agtgtggcgt tcccaaggag ccttaaaaaa
      721 acccattact gcgcttttgt tcggcagggt taccattttt gaagcgcaaa agcgtgccga
      781 ctgggaagtc ggcgccacct tgactcccgg aaaaacttct tccgtgtcat ctgtgccctc
      841 catgtgaaac cgttcttttg gctcttcgat acattacttg aagcccactg atcatccgag
      901 aaatacaaag taacataaga ggaataaaaa gagttcgaca gatggccgat tatgcgatta
      961 gagcggatta gtgaaacggg ctaagggttt acgaaattat tacgatagaa gatgttaatt
     1021 aagtacgtct tgtaaattgt cgaagagccg ttctttttct tttaggacat gccaacttaa
     1081 aagttggcgt tcccagggcg ctcccaagga gccgtaaaga aacccctacc gaatgagaaa
     1141 tcggcgccgc taggcacccc caagaatttt ctctccatgc tcgccgcgtg aaaccttctt
     1201 ttcttttttt accatatttt aatacgaaaa gctatgccaa atgataaatc agcgtttaca
     1261 gctaacgcaa attcgcgatg gtaacaaaat cgtgattaag ttcacttcat tctctctttc
     1321 aaatgcctta cctaaatact tgaatcataa aacgagcatg gtataatcat gcttgtattc
     1381 ctttcttaac ttaagagagg ggaggcgagt cgtagttcaa acgcgacgaa cctcctcaaa
     1441 aacgaaaagg agatgatgag gatgatgaaa ctcaagagct tacgagcgaa gctgctgttg
     1501 tactttatac cagtatcagt ttctgtgctt gtgatctcag gtttcattat tgccctaagt
     1561 gtgagaacgg ggatcttaga acagactatg gaatcaacgg aagaaacgct ccatacgagc
     1621 gtgcgaattg tcgaagagat gttggctgga attaagacag aactccgtaa cctagccaac
     1681 actgacgtgg tcaaatcttt cgatcccacg cgttttgttc ctaggtttgt ggaaactatt
     1741 aaacaaggcg aaggactcta tgaaacgttc tttcttgccg acccaaccgg aaaagcaatt
     1801 atggaaacgg gtgaggccgt cgatatccgt gatcgccctt actttcagga agtaatgttt
     1861 caaggaaaga agttcgcgat aaacgacgga atgatcagta ggtttgccca tatccctgcg
     1921 tttggcgtcg cttaccctgt ctatgactac actggtcagc tcattggcgt gttaggcgcc
     1981 aatgttgcct taaaaacttt gagcgaaaaa gtgtgcgcaa ctaagatagg ccaaacaggg
     2041 tacatcttcc tggtggatgg ttccgggacg gtgatcgcgc atccggacac agatttaatt
     2101 ctaaacctca atttgttgga agggacgaag tccaagtaca aaggtctgga agaagccgga
     2161 agggaaatgg tcagcgggcg tgaaggtttt gggaatatcg ttcttccctc tggagaaaag
     2221 caaatccttc attacgattc tattcctaat actccgaact ggaccgtagc gggagtcatt
     2281 tcagaagcgg aggtgaccca aaagagcgct tacttcctcg taactgtagt cgtgatattc
     2341 gccgtcatcg tcggcgttat cgtgctcatc tctatgtttg tgggctcgtt catctccaaa
     2401 tctttgaaga ggcttagcgc gcaagtggat caattcggga aaggagactt aaccacggtc
     2461 ttcgaggtaa aaggccgtga tgaaatcgcc caaattgctc aagctttatc cgagatgggt
     2521 aacgatctcc gagaagcgat ggtttctata ggagaggcat cacaagagat aaaagcggct
     2581 tcgacggatc ttgcagcgat ctctgaagag cagctcgcgg gatctgagga gctatccagt
     2641 caatcggcaa gcgtgaactc aaacttacag aataccgtag cggcaataga agaggtgaat
     2701 tcaggagtgg atgaggtggc cgcgagcgcg cagaacgttt cgaaaaccgc tcaggcgctc
     2761 tccacagaga acaacaagac ggcagatgga tctcgagaag gcgggcagat gatctcagac
     2821 gtagtacggc agatcgaagc gacaacgcaa cagactctac tgacagcaga aagggttcag
     2881 aagttggcgg agaattcgaa gagcgtgggt gagatcgtcg atacgatctc ctcgattgcg
     2941 gagcagacga acctgctggc gctcaacgcg gcgatagaag cagcccgggc cggagaggca
     3001 ggccgcggct ttgcggtggt cgcggacgaa atccggaagt tggccgaaga aagcaaaagg
     3061 gcaacgtcca acatcggagc gatattgaaa gaaataaaaa aagacgccga agaatcctat
     3121 caagcgactg acaagaccgt tagtttggta aaagacgtca acaccaaatc acaagcggtg
     3181 gaaaagcaat tcaaacactt gctggacatg gtagaaaaga cgacagggat gatcgaaaac
     3241 ctaacgggaa cctcggaaga gcagggagcc gcggcagaag agatggcatc tgcgatggac
     3301 acttctgcga aagccgcgca cgagatttcg gaacagattc ggcaaatggc gcagggtatc
     3361 gaacagcaaa ctcaaggcgc gcaccagatc agttccgatg ccgaagaact tgattcattg
     3421 gccgaaaacc tcgatactca gatcaagcgg tttaaagtat aagaatagga agatatacga
     3481 gacgccggcc tttggccggc gttttttttt gttgggagcg ctctgggaac gccaactttt
     3541 taagttggca gggtttcggg ttttcaaaga agaaaacaca acctatgcca cacttaagtg
     3601 tggcgctccc aaggagctct aaaaaaacca cgccggctgg gaagtcggcg ccagtaggtc
     3661 gccgccacca ggtacccctg ggccgccctg gcggtttcac ggtacaccag gataagctga
     3721 aaagtaggca atccgccccc ccttgacttc attaagaaat gagagtacaa tatatgtaca
     3781 ggtgagcata tgtaatgaaa tatgagaaag gagtagcgaa gatgagcggc ttagaaggaa
     3841 attgcgaagt tcataacccg cacgatgagc tgtgtgaccg catcgctgaa aagcttcccg
     3901 aggaaagtca gatcgcgcgc gcggcggagt tgttcaaagt tttcggcgac ccgacgcgtc
     3961 taaaaatact cacgctactt tccatttcgg aatgttgcgt gtgcgatatc gccaaactca
     4021 tagacctctc gcagtcggct gtatcccacc agttgcggat actcaggcag acccgattgg
     4081 taaaataccg gaagcaaggc aaagaggcgt tctattcctt ggatgacgag cacgtgagtg
     4141 agattctgtc actcggattc gagcacgccg gcgaggaaca aggaggcgga cgatgactca
     4201 gtctttctcc gtggaagggt tgacctgcgc ccattgcgcc acgaaaattg agcgaaaggt
     4261 gcgcgaagcg ccggatgtgc tgcgcgcgga actgaatttc gtggcgcgca cgctgaccgt
     4321 tgaaacgcgc ggagaggagt tacctgccaa cctgcaaggg ataatccaat ccgtcgaacc
     4381 ggaagctcgg atcttgaaac gctcggaact accggaaacg ccgctcttgg agcgattgga
     4441 agacgaaagg cgcgcgatgc gcatagacct cgcgaggctc gtcgcgggca gtgtgttatt
     4501 tttggtttcc attctgacct ccttctcctc cagatacgcg ctggggctga gcctgatcgc
     4561 ctacggagtg atcggctacc cggttttgct cgcctcttgg aaaaaagcga tgaaaaggaa
     4621 cctatttaac gagaactttc tgatgacact cgccaccatc tgcgctttcc tcattgggca
     4681 acccgcggaa gccgtcggtg tgatgatttt ctatgaaata ggcgaacttc tgaatgccta
     4741 cgcgctcaat cgctctcggt atcgaatttt cggtttgttg acccacgaac ccgaaaccgt
     4801 aacggcgata aaagagggaa acgccactgt cgtgcatcct cgagaggtct tgcctggtga
     4861 gtctttcatc gtgaagcccg gcgagcgcgt tcttttggac ggagagatcg aaaaaggcga
     4921 agccctgatc gatacctctt cactcactgg agagcccgtt ccaagaacgt ttgggaaggg
     4981 agacgaagtg ctgtcgggtt ttatcaatac gaatgcaacg ctacgcatcc gagcgacaaa
     5041 acggtatgaa gactccaccc tctcgcgcac gctctccctc atagaagaag ccgccctgaa
     5101 aaagtcccga accgagcgct cgatttcgcg gttcgcttcg gtttacaccc cggcggtcgt
     5161 attcggcgcg ttggcgctcg cgacgctccc tccgatcctg tttgggctcc ctttcgaaag
     5221 ttggctatac cgcgcgttgg tgctgctggt gatttcctgt ccctgcgcgc tcgtgctttc
     5281 ggtcccactt gcctctttcg tagctatcgg taagctctcc aaacaaggaa ttctggtgaa
     5341 ggggagcgat ttcttccgta cgcttaaaaa agcgaaatcc gtcgtgtttg acaaaacggg
     5401 aaccctcacc gccggccttc tgaaaatcga ccgcgttacc gctttccttc cccatacgca
     5461 ggagcaggtg attcattatg ccgccatcgc cgaataccac tcttcacacc ccattgcgag
     5521 ggccttacgt gacggtttac ccgaaggctt ctctcctgac gagatcacgc agtaccagga
     5581 atatgcgggg tcaggtcttt ctttgaacta cagaggtacc gaaatccttg tcggaagcgc
     5641 tcggttcctc gagagccgcg atattcggat caaaggccag gcggatgcgc cggaggcgct
     5701 cacgacggtt cttgtcgctg tcgacggcgt tcacgcagga acagtttact tttcggattc
     5761 tgaacgtcct gattccgctg agctcatcgc atttctacga gacagggggt taaagacctt
     5821 cttgctgacc ggggatcgcg cgcccgcggc gaaagaggtc gcccagcgcc tcgggctaga
     5881 cggctatttc tcggagttgt ctccgaagct gaagatcgaa gagtacgaaa aaatccggga
     5941 tcaaacgggg cagtccctgt ttatcggaga cggcgtcaac gacgcgcccg tcttgcgacg
     6001 cgcggacgtc ggcgtcgctt tcggcggtaa aaacagcgac attacgctcg aatctgccga
     6061 catcgtgatc ccgggcgaag agatcaaaaa agtcaaaacg ctatttgaat ccgcagaccg
     6121 tcacgccaag atcctttggc aaaacatcct cttgatttta gggatcaaag ggctctttct
     6181 gttgttggga ggtttcggtc tttcatcctt atgggaagcc gtgttcgcgg atgtcggcgt
     6241 gacgctgctc tgcgttctca acacgttccg tctttcctaa caccggggcg ctccgaaaat
     6301 agagtgtctt tcattcggag cgccaggaca ttgggctagt aatacgtgat gatcagcttt
     6361 aattccgtat cgaaggcgcg taccgtcctc caaagggcct ttttttggac gacgtggtca
     6421 ctccctacgg cgaaaaacga gtaagcgcca ttcccagaag atttcacggt catctggcga
     6481 cccagggttc tgagctcttc aaaaggctca taaaccggat cggtgaagag gttccgccct
     6541 atctcccacg tgtcgggatc gtagacgatc gtccccgtag gatgcatgat ccagaagtcg
     6601 gaatccgtat gttgccccgc atccgccaga atagagaaaa atctttccgg gcgtatcaag
     6661 agactgatgg aaccagcgta tccgccgaat tcgtcgaaaa ccggccactg gatgtccatt
     6721 gcgagaaagc cttctaccgc cataaagatg tcgctgatga caggcctttt cgtttcacgt
     6781 aaacgaatca cttgatcctg gccgctgatg tcggcacctt catgatttcg atactcttcg
     6841 ggttcgaccc atttcatgat cccatccgga ttcacgaaag acacatcgac gatcgcgggg
     6901 tccaacgtcc gtaattctgt aagaatagac ctcgtcaggc tttcgttcgc aaaggagtag
     6961 gtcagccgtt cggccccttc ttttaacccc tcggtcaagc gggcgatcga agcggcggtc
     7021 cttttttgga tggattccag ctgactttcg gggctgtccg ccgtgctctc gagttccccg
     7081 gatttgacgg tgagaaaggc cgtccaaccg gtttccaagc tcttcctata aaaagcgaca
     7141 taggtcccct tcggactcgt aaaaaagaat gagccttccg gttggctaga cttcggcatc
     7201 tgtgccaaaa gggcgcccat ctcgtgctct tccggatagg cgatggcctc caagttgctg
     7261 aaaaccaatt tgcctgtcca gtcgaaaaaa taatatgcgc aaccctcagg cagaggaatt
     7321 tgacccctca gacggatcac caaggggttg agatagaggg acaggccgaa gtaggaggta
     7381 agggttgacc cctccataac ggggatgccg atgactaatg agggttcgcc cgtcgagcgg
     7441 ctgtacacgg aagcgccttt aacggtctgc cctttttcca agagctggta atagtcccgg
     7501 tctttgaggt tcaggccggt ccagttacgg gtcacggtat agtatccttc aggaatcccg
     7561 taaatcccga cgcccggata ccgttgctcg atcacttgca gggagggctt cacgatctcc
     7621 cactgcgaac gcaagggcgc aggtaagttg gcgagcgtcg acaggctgtt ttcggctcct
     7681 ttgattgtct cttcgaacaa agaaacgatg gaacgtaggt agtatcgctc cggaatcttg
     7741 tcgtatccga caatacgatc aaatgatacc gcgctgaaaa gcggctcttc caatccgact
     7801 tctcgagccc tttggacatt tatcatccgt tctcctcttt cgagagccct aagcgggaga
     7861 gaggtagggg cggtttgatg gagcagtatc tcacaagcca gtcgtcccgc ttcgtacccc
     7921 tgtaggtagg gcgactgcac aaccccaccc aatatgccct cttcgaccat atagtccaag
     7981 agcgcgaagg tcgggaccgg gctgttgcga gcggtccaac cccctacctc ttcggtgggt
     8041 acggtatggc cagcttgatc ggtcaacacg cggtaagcgg ccaaaaagat agcgtcataa
     8101 cgggtttgcg attgcaaaat aaccgttttc cattcttcga aggtcgaaac cgatatcgtc
     8161 tccagggttt caacaccgga gagcaccgcc tttttcgcgt gttcgagtat cccttcggtg
     8221 gtgatcccct ggtcgccgag gaagagaacg cgacggacgg gaggaaaaaa ggtccgcgcg
     8281 aattgaatag actcttcgaa gaacgggatt tccaaaacgc cagtgacatt ttccctcgga
     8341 tacccgtagc gttccgggaa ctggttgacc ccgcaaaaca cgattggtac ccctcgcatc
     8401 tcctttccga ggtattcttg cgcgtcatcg tcgcaaagga tgaggaggtc cggtttctct
     8461 tcttctatcc gcgttttgac gagttctccc gccttgatct tccaatccgg ttgatttttc
     8521 aggcgggcgt tcaagtaata agcctgtatc tgtgtatcgc ttccacaaag ggcatctcgc
     8581 accccttgat cgagctgctg gctccatatg aaatcggggt cgtagctctg gacgatgaaa
     8641 acggttttca tcgaaaaacc catccctagg cagagcaagg tgaaaaagac cacagcaaag
     8701 cacaatcgac acttcatact atccgctcct ttcggaaaat tgtgggtgcc aaatatcact
     8761 tgaagtagga ggccaaaggc ggtctgtggt aaaatggagc cggtgaagat gtgatgatga
     8821 aaaagagaaa gataaccggt ttcattctcc tgctatgtac actgtccgtc ttctcgtata
     8881 cgctcctgga acaacgcgac tggttgttcg acggcgactt cattctccta gaaggccaac
     8941 tcgttttggg aaaaccgatt gaaatctcgc tgctgaccct ctatcccgtt tttttaatcc
     9001 accgcctcga ctatcggttt aattcttctg atgagtctga gaccgcgccg cggcttttag
     9061 gtatggagat gactccctac gcgctcatcg tgataggtga caaggaacaa catatccgtc
     9121 tcataagcgc ttcggagggc gccacggata ccgaagcggt gaattacgcc gagttgatgg
     9181 cccttagcga gacgatccgc aaggagctct atcgaaaatc ccctgccgaa aacgacccgc
     9241 tcaacgattt cgaactcttg atgatccacg tcttcgccat aaagaacgtt ctaccctaaa
     9301 tcgtggcggg ttataccatc ggggtccttt tttcggattc ggtggcggag aaaaaaggga
     9361 atgcggttat cctgtaaagt ttcgcgaaca ggttaagtgg aaacacggcg atggcggtgt
     9421 tgatttc
//
LOCUS       DAOPXN010000080         9373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7372_length_9373_cov_5.607856, whole genome shotgun sequence.
ACCESSION   DAOPXN010000080 DAOPXN010000000
VERSION     DAOPXN010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7372_length_9373_cov_5.607856"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2895
                     /locus_tag="PKM49_05825"
     CDS             <1..2895
                     /locus_tag="PKM49_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HNY82368.1"
                     /translation="RSIRQEATAFQPDRFRLERANQTLPLHGGYEVLKTLRAQQREDS
                     NQLEGYRSALTELEEKVREAEREMEERQGAYERARGDKEQGMRLIVGVRALDLRIAES
                     DRRINDLDREARRLNVMIQQLTSARAQKAKESEACSKEGERLDLWFSEHRGDEPLAEH
                     FSAIRQSVEGYKALAASIDPLRERLRQTKARRETAEQRRQNLSERWQDLVGRLNQAQK
                     AESDQAAVLQAKLGGRTLEEMRSQLDRLKTRKNWLERLIDPLRRMISGAERIAEIVSL
                     LIGLDQENEDCEEALGRFVKSKERAEQVSEALKRLIERIRRAHDLESERKKLEDNVPC
                     PLCGALEHPYARENIPTPDPEESELENVNGEIRELTKKIDALRIKIAQNHAKKAHLLA
                     ERAEKQTALETDAGRRDELYRHLGIDPREDLHENLAVVNGTLESEAPILEGLSSEISA
                     LESVGKLLEEQRRVVQRLKDDVRKAENELNAAVQEESLNKQAYEQAEETYAEKERVYG
                     ETRQRLLESLIPYGISEVSPENADTVLEALDSKRRLWNENKRRKETLTTNQARIDTEI
                     ENLTITIQTREAEIVEKRAVLERETEAREDLRSSRRELFQDRNTDTEEKRLSDALDSS
                     ERALNASRETLSQRLSDRQSMRELKEKSDRSVRERAAEIGNRESNFQTDLISAGFSDE
                     VDFLNACLEEPQRLELSLRDSELRTRESHLQEALRALEQDLHTEKERRLSEEPYETLL
                     SQSTQYEERRQSLQEEIGALQQRLSAHEQAMRERAEQLTAIAAQTRELARWEELHALI
                     GSADGRKYRNFAQGLTFVTMVAYANDQLRKLTDRYLLSCNPDIPLELVVQDLYQAGVS
                     RSAKNLSGGESFLVSLALALGLSQMSSRNARVDSLFLDEGFGTLDEEALETALATLSG
                     LRQEGKLIGVISHVAEIKERLPVQIEVIPLGGGISALKGPGCRRVEGRIA"
     gene            3315..4664
                     /gene="gcvPA"
                     /locus_tag="PKM49_05830"
     CDS             3315..4664
                     /gene="gcvPA"
                     /locus_tag="PKM49_05830"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HNY82369.1"
                     /translation="MGYRYIPHSEDEVRSMLQRIGLTKIEDLFRDIPEQFRLGRLALP
                     EGKVEADVIRQMNDWSKSNLNANDCAIFMGGGVYQHLIPSAIPFLAERGEFLTAYTPY
                     QPEVSQGSLQVFFEFQTMICELTGMEVGNASMYDGASAAAEAVLMACRIKRKMKFAVS
                     EAIHPEYLEVIRTVAEPMGIEITVFPYNRDTGMTDPEELKAVLNEDFAGFLAGYPNFF
                     GVIEDIRALREAAPDALMVVSVNPIALGVLKAPGDFGADIVCGEGQPLGGSLYLGGMS
                     LGLLATKKEYIRNLPGRIIGETKDIDGHRGFVMVLQTREQHIRRSKATSNICSNHAHN
                     ALVATLYLALLGKQGLKAVALQSTQKAHALHARIERMDRYRPRFSGPIFNEFVVESNV
                     EPEALQKELMEQNILGPLPLKRFYRESFYDHLCMFAVTEANRIDDIHFLARRMEEIV"
     gene            4661..6148
                     /gene="gcvPB"
                     /locus_tag="PKM49_05835"
     CDS             4661..6148
                     /gene="gcvPB"
                     /locus_tag="PKM49_05835"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNY82370.1"
                     /translation="MSSRLSGYRLDDALIFEKSRAGHTGYEILAEEIPDFTLQAMFPE
                     AYIRSQDAELPEVPEVDVVRHYTNLSKLNHSVDNGFYPLGSCTMKYNPKLNEAVAALE
                     GFQQIHPYQPESSIQGALALMYALQEALKVVSGMDAVTLQPAAGAHGEYTGMRMIYAY
                     HQDRRDFKRKKVIVPDSAHGTNPATAVMCGYETIEIPSTADGRVDIEELKKVLGPDTA
                     AIMLTNPNTLGLFENQIYEIAQMAHEAGALLYYDGANFNAIMGKVRPGDMGFDVLHFN
                     VHKTFSTPHGMGGPGSGPVGVKECLRPYLPVPVIEKHPETGRYWCNYKRPKSIGKVRA
                     YFGNFNVLIRTMAYILSMGGEGLTKASEMAVLNANYLRVQIAEFLEVPHNEICMHEFV
                     AEGTRLKEWGIKTLDIAKGLLDYGIHPPTIYFPLIVHEAIMIEPTETETKETLDRFVS
                     VMKKLVDLAKRNPDKLKDAPITTPVRRLDETSATKNPVVRYQKKG"
     gene            complement(6155..7066)
                     /locus_tag="PKM49_05840"
     CDS             complement(6155..7066)
                     /locus_tag="PKM49_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HNY82371.1"
                     /translation="MKYEKPIKIGAHMGISKGFHLVPKETVEEVGGNAFQIFNHSPRF
                     WKFSPPSDEQVALFHQSMVQHRLDYENTLVHSSYLVNLASPKDEVYEKSIETMIQEMQ
                     ICDRLKLPYLNVHPGSHLGEGEAFGFSRIVNALNRIFIDTPDLPTMILLEGVSPKGGN
                     IGYYPSQIKEIIDGVHPDFQHRLGYCYDTCHGFDAGFDITQPEGVQGLIDTIREDLGW
                     ERLKMIHLNDSKFPLNARQDRHARIGEGTIGRDGFKTFFSFKEWQDIPLLLETPGDNP
                     EHREDIEVIREILHQIDPADGWDWRRG"
     gene            complement(7063..>9373)
                     /locus_tag="PKM49_05845"
     CDS             complement(7063..>9373)
                     /locus_tag="PKM49_05845"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82372.1"
                     /translation="FPAIILVLAVTNPLHRLLWPNITLRAVPGGVAGVFEHGIFFWFS
                     VIYNYSMALAAIFLLSRHFFFSKDTGIPTASKVLLLASFCPLVANIVYAFVPSLIGGF
                     DPTPLSFTAMTFLLVLASFKFRFLDLIPIAWERVIYTFEDGVLALDRHQRVVGWNPAL
                     EEWLGLSLENRGEVCEKALRNWPRLTELLANAVNVTKTIELTTRDGKERVLELRITPF
                     SDFRGRLNGKIVIFQDVTAQKLVLRQLESARNQAEAANRAKSAFLSAMSHELRNPLQG
                     ISGIVFLLAKSRLDSNQKQQMEHLESAMRTLSGIINNILDFSKIESDKLLLESVDFNL
                     RLVIQDVFYLFDSLATEKGLTMVLKIDDSLPTECRGDPMRIKQIFINLVSNAVKFTPS
                     GGHIILTARGSPKENAQDGKQTMLFEVSDTGMGISEEKGKHLFEAFYQGDAAVARQFG
                     GTGLGLSIAKDLVEKMGGRISFESRLGFGTTFSFTLRLPPASSPLTVESKALETGSSS
                     FVGKTVLYVEDNAVNRMILTEILTSFGLSVSAVPNGKAAYEAAKRNTFDLMITDIFLP
                     DMEGYELTRMLRGIRTPLWPTIALTGNSSWEARDKALKAGMDVFLTKPVSPYRLREEI
                     SRLLGIDSSPASIATLTETDVTESFPGSLPGIDKQTAMEMVDGDLEVYREALKLFIRS
                     IRNLEETFRDALSRGCFSEAGRTLHLTKGAAMAVGANTFARSCDDLEKSMKERMPETL
                     EPILGQWLRQLNELLKSKEIENLIREGPR"
BASE COUNT         2332 a         2408 c         2670 g         1963 t
ORIGIN      
        1 cggtcgattc gccaagaggc cacagccttc caacccgacc ggttcagact cgaacgcgca
       61 aaccaaactc tgcccttgca cggcggctat gaggtgttga aaacgttaag ggcccagcag
      121 cgagaggact ccaaccaatt ggaaggctac cggagcgcgc ttaccgagtt ggaggaaaag
      181 gtacgagaag cggaacgaga aatggaagag cggcaaggcg cctatgaacg cgcgaggggt
      241 gataaagaac agggtatgcg actcatcgtg ggtgtcaggg cgttggacct tcgtatcgcc
      301 gaatcggacc gtcgtattaa tgacctcgat agagaggctc gacggctgaa cgtgatgatc
      361 caacaattga cgagcgcgcg ggcacaaaag gcgaaagaat ccgaagcctg ctcaaaagaa
      421 ggggaacggt tggacctgtg gttttcggag catcgaggcg acgaaccctt agccgagcac
      481 ttctccgcga tccgacagtc agttgaggga tacaaggcgc tcgcggcgtc catcgatcct
      541 ttacgagaaa ggctgcgtca aaccaaagcg cgacgagaaa ccgcggaaca acggcgtcaa
      601 aacctgagtg aaagatggca agatttagtc gggcggctga atcaggcgca aaaagccgaa
      661 tcagaccaag cggcggtcct tcaagccaag ctcggtggaa ggacactcga ggagatgcgg
      721 agtcagctgg atcgtttgaa aacacgaaag aactggctgg aacggctgat tgaccccttg
      781 aggagaatga tttcgggagc cgaaagaatc gccgagatcg tatccctatt gatcggtctg
      841 gatcaagaaa acgaagattg cgaagaagcc ttgggccgat tcgtcaagag caaagaaaga
      901 gcagagcaag tctccgaagc gttgaaacga ttgattgagc ggatacggcg ggctcacgat
      961 ctcgagtccg agcgaaagaa gcttgaagac aacgtccctt gcccgctatg cggggctttg
     1021 gaacacccgt acgcgcgtga gaacatcccg actccggatc cggaagagtc cgagctcgaa
     1081 aacgtgaacg gggagatccg agaactgacg aagaaaatag atgcgttgag gataaagatc
     1141 gcacagaacc acgcgaaaaa ggctcacctg ctcgccgagc gtgcggagaa acagacggcg
     1201 ttggaaaccg acgccggtag gcgcgacgag ctctaccggc atttgggtat cgacccccgg
     1261 gaggacctcc acgaaaacct tgcggttgtc aacgggacgc tggaaagtga ggcgcctatt
     1321 ctcgaagggt tgtcttccga aatctccgcg ctcgaatccg taggaaaact tctcgaagaa
     1381 cagcggaggg tcgtccaaag gcttaaggat gacgttcgca aagcggagaa cgaattgaac
     1441 gccgcagttc aagaggagag cctcaacaag caggcttatg agcaagcgga agaaacgtat
     1501 gccgaaaaag aacgcgttta cggagaaacg agacagcgac tcctcgagag tctgattccg
     1561 tacggtattt cggaggtctc tcccgaaaat gccgatacgg tcttagaggc gttagactcg
     1621 aaaagaaggc tctggaacga gaataaacga cgtaaggaaa cgcttaccac taatcaggca
     1681 agaatcgata ccgaaatcga gaatttaacg ataacgattc agacacgaga agcggaaatc
     1741 gtagaaaaac gcgcggtttt agaaagggag accgaggctc gggaagacct ccgctcgtcg
     1801 aggcgggaac tgtttcagga tagaaacacg gacacggagg agaagcggct atccgacgcc
     1861 ctcgattcct cggagagagc gctgaacgcg tcgcgggaga ccttgagcca acggttgtct
     1921 gatcgacaat cgatgcgcga actcaaggaa aaatcggacc gttccgttcg tgaaagggcc
     1981 gcagaaatcg gcaatcgaga atcgaatttc caaactgatc tgatttctgc cggtttctcg
     2041 gacgaggtag actttctcaa cgcttgtctt gaagagccgc aacggcttga actgagtcta
     2101 agggattcgg agctcagaac acgagagagc catctccaag aagcactgcg agcgctggag
     2161 caggatttgc ataccgagaa agagagacgg ctgagcgagg aaccttacga aacgctgctc
     2221 agtcagtcca cgcaatatga ggagcgccga caatctcttc aggaagagat cggagccctt
     2281 cagcagagat tgagcgccca cgaacaagcg atgagagaac gagcggagca actgaccgca
     2341 atcgctgcgc aaacacgcga gttggcccga tgggaggaat tgcacgcgtt aatcggttcg
     2401 gccgacggga ggaagtacag gaatttcgct caagggctga cctttgtcac gatggtggcg
     2461 tacgcgaatg accagttgcg caagctcacc gatcgatacc tcctctcgtg caacccggac
     2521 attcccctgg aattagtcgt tcaagacttg tatcaagcgg gggtttccag gtccgcaaaa
     2581 aacctttcgg gtggggagag tttccttgtc agcttggcgt tggctttggg actctcgcag
     2641 atgtccagcc gcaacgcgcg cgtcgactcg cttttcttag acgaagggtt tggaacactt
     2701 gatgaagaag cgctcgagac cgcgctggct actctatccg ggttgcggca ggaagggaaa
     2761 ttgatcggcg tgatctctca cgtggctgag atcaaagagc gcttacccgt tcaaatagag
     2821 gtaattccgc ttggcggcgg catcagcgcc ttaaaaggcc ccggatgccg gcgcgtggag
     2881 ggcaggatcg cctaaggtct ttctttcgcg ctcgcctcat tgtttaccca cgcccgattc
     2941 agtgctgggt gtactattga ggcgcaacaa aaaatagaaa gaggaggaga cccgcctttt
     3001 gtctttttta cgccgcgatc acgtcgcata aaagcgtatc gcgccgttgc gaaaagaagg
     3061 ggcgcttacg ccgcgatgac cgtgtgggtt atcgcggggt aagtggtttt tacgaccccg
     3121 cgatccgcct tttgtctttt ttacgttgca atgactacct ggactaaaaa cgtacggtat
     3181 ttctgacctt gtttcttgat agcacctcat aacttgaccc acactccaac tcagaataga
     3241 cgcctaaaag tgatataatc ggcgggaaag ataagaagac cgggtagagg ccggtctcac
     3301 tataaggagg tactatgggc tatcggtata ttccccattc cgaagacgag gtccggtcga
     3361 tgcttcaacg aatcggcctg accaaaatcg aagacctttt ccgcgacatt cccgagcagt
     3421 tccggcttgg aagattagcc ttgccggaag ggaaggtcga ggcggacgtc attcggcaga
     3481 tgaacgactg gtcgaaaagc aacctcaacg ccaatgattg cgccatcttt atgggcggcg
     3541 gtgtctatca gcatctgatc ccctccgcga ttccgttcct cgctgagcgg ggcgaattcc
     3601 ttaccgccta caccccttat cagccggagg ttagccaagg gtctttgcaa gtgtttttcg
     3661 agtttcagac gatgatctgt gagttgacgg gtatggaggt cggcaacgcc tctatgtatg
     3721 acggggcgag cgcggcggcg gaagccgttc tgatggcgtg ccgtattaag cgaaagatga
     3781 agttcgccgt ttccgaagcc atacaccccg aatatctcga agtcatacgc acggttgcgg
     3841 aaccgatggg catcgaaatc accgttttcc cctataatcg tgatacgggc atgacagatc
     3901 ccgaggagtt gaaagcggtt ctaaacgaag acttcgccgg ttttttggct gggtacccca
     3961 actttttcgg ggtcatcgag gacattcggg ctctgcgcga agccgcgccg gacgcgctga
     4021 tggttgtcag cgtcaacccg atcgctttgg gcgtgctgaa agcccccggc gacttcggcg
     4081 ccgatatcgt ctgcggagaa ggacagcctc tgggaggttc cctatacctg ggcggtatga
     4141 gtttagggct cctcgcgacg aagaaagaat atatccgaaa cttaccgggg cgcatcatcg
     4201 gggaaacgaa ggatattgac gggcaccggg gattcgtgat ggtgctgcaa acgcgcgaac
     4261 agcatatccg taggtcgaaa gccacttcta acatctgttc caaccacgcg cacaacgccc
     4321 tcgttgcgac gctgtatttg gctttgttgg ggaaacaagg gctgaaggcg gtcgctttgc
     4381 aatcgaccca gaaggcccac gcccttcacg cccgaatcga gcggatggac cggtatcgcc
     4441 cgcggttttc cggccccatc ttcaatgagt tcgtcgtcga atcgaacgtg gagccggagg
     4501 cgctgcaaaa agagctgatg gagcaaaaca tcctggggcc gttgccgttg aagcgctttt
     4561 atcgagagag cttttacgac catctgtgta tgttcgccgt gacggaggcg aaccggatcg
     4621 acgatataca ttttttggca agacgaatgg aggaaatcgt atgagcagca gactgagcgg
     4681 ataccgtctg gacgatgcac tgatctttga aaagagcaga gccgggcata ccggctacga
     4741 gattctggcg gaagaaatcc cagacttcac ccttcaggcg atgtttcccg aggcgtacat
     4801 tcgttctcag gacgcggagc tcccggaagt gcctgaagtg gatgtggtcc ggcactacac
     4861 caacctatcg aaactcaatc actcggtcga caacggattt tacccgctcg gctcttgcac
     4921 gatgaaatac aatcccaagc tcaacgaagc cgttgcggcc ttggaaggct ttcaacaaat
     4981 ccatccgtat caaccggagt cctccataca gggcgccttg gcactgatgt acgcgctcca
     5041 ggaggcgttg aaggtcgtat ccggcatgga tgcggtcacg ttacagccgg ccgcgggcgc
     5101 acatggagaa tacacgggta tgcggatgat ctacgcctac catcaggatc ggagagactt
     5161 caagcgaaaa aaagtgatcg ttcccgactc cgcgcacggt accaaccccg ccaccgccgt
     5221 gatgtgtggg tacgagacca tagagatacc ttccaccgcg gacggtcgtg tggacatcga
     5281 agagttgaaa aaggtccttg gcccggacac cgccgccatc atgttgacga acccgaatac
     5341 tctggggttg tttgaaaacc aaatctacga gatcgctcag atggcgcacg aagccggcgc
     5401 gttgctttac tatgacgggg cgaatttcaa cgcgattatg gggaaggtgc gcccggggga
     5461 tatggggttc gacgtgctac attttaacgt ccacaagacc ttctcgacac cccacggtat
     5521 gggagggccc ggtagcggcc ccgtcggcgt gaaggagtgt ttgcggccgt acttgccggt
     5581 accggttatc gaaaagcatc cggagaccgg tcgctattgg tgtaactaca aaaggccgaa
     5641 atcgatagga aaagtgcggg cgtattttgg caatttcaac gtactgatac gaacgatggc
     5701 ctatatcctg tctatgggcg gcgaaggctt aacgaaagcg agcgagatgg cggtcttgaa
     5761 tgccaactat ctgagagtac agattgcaga attcttggaa gttccccaca atgagatctg
     5821 tatgcacgaa ttcgtagctg aaggcactcg gctgaaagag tgggggatca agacgctgga
     5881 catcgcgaag ggcttattgg attacggcat ccatcccccg acgatctatt tcccgctgat
     5941 cgtccacgag gcgatcatga tcgagccgac cgaaacggag accaaagaga cgttggatcg
     6001 gttcgtatcc gtgatgaaaa agctggttga cctagccaaa cgcaatccgg ataagctcaa
     6061 agacgcgcca attaccacac cggttcgtcg gctggatgag accagcgcga ccaagaaccc
     6121 ggtggtccga tatcaaaaga aggggtaagg cggtttagcc ccttctccaa tcccagccat
     6181 ccgcgggatc gatctgatgg aggatctcgc ggatgacttc gatatcttcc cgatgttcag
     6241 gattatcccc cggagtttct aaaagaagcg ggatatcctg ccattcttta aaggaaaaga
     6301 aggttttaaa cccgtccctg ccgatggtgc cttcgcctat acgcgcgtgc ctgtcctggc
     6361 gcgcgttgag cggaaacttg ctgtcgttga gatggatcat cttcaagcgt tcccagccca
     6421 ggtcctctcg gatggtatcg atcagccctt gaacgccctc cggttgtgtg atgtcgaagc
     6481 cggcgtcgaa cccgtgacaa gtgtcgtaac agtacccgag gcgatgttga aaatcgggat
     6541 gcacgccgtc gatgatttct ttgatttgag aagggtaata cccgatattg ccacctttgg
     6601 gagaaacgcc ttccagcaag atcattgtgg gtaaatccgg cgtatcgata aagatacggt
     6661 tcaacgcgtt cacgatacga gagaagccga aggcctcccc ttctccgaga tggcttcccg
     6721 ggtgtacgtt caaatagggg agtttgaggc ggtcgcaaat ttgcatttcc tgaatcatcg
     6781 tttcgatcga tttttcataa acctcgtctt tcgggctggc cagattgacc aaataactcg
     6841 aatggaccaa ggtgttctca tagtccagtc gatgctgaac catactctgg tgaaagagcg
     6901 cgacctgttc atcggaaggt ggcgaaaact tccaaaaccg cgggctgtgg ttgaagatct
     6961 ggaaggcgtt gccgccgact tcttcgaccg tctccttcgg cacgagatgg aaacccttcg
     7021 agatacccat atgggcgccg atttttatcg gtttttcgta tttcaacgcg gtccctcccg
     7081 gatcaggttt tctatttctt tcgatttcaa caattcgttt aattgtcgca gccattgccc
     7141 gaggattggt tcgagggttt cgggcatccg ctctttcatc gatttttcga gatcatcgca
     7201 cgagcgcgcg aacgtgttcg ctccaaccgc catagccgct cccttagtga ggtggagggt
     7261 tctaccggcc tcagagaaac acccccggct caaggcgtcg cggaaggtct cttctagatt
     7321 ccgtatcgaa cggatgaaca gctttaaggc ctcccgatag acttcaaggt ccccgtccac
     7381 catttccatt gcggtttgct tgtcaatacc gggcaaagaa cccggaaaag actccgtcac
     7441 gtcagtttcg gttagggtcg cgatcgatgc gggtgaggaa tctattccca acaaacggga
     7501 gatctcctcg cgcaagcggt aaggagaaac cggcttcgtt aggaagacgt ccattccggc
     7561 ttttaacgcc ttatccctgg cttcccaaga actgtttccg gtcagtgcta tggttggcca
     7621 aaggggggtt cggatacctc gcaacattcg ggtgagctca tacccttcca tatcgggcaa
     7681 gaaaatgtcg gtgatcatca gatcaaaggt atttcttttc gccgcctcat aagcggcttt
     7741 cccgttcgga acggctgaaa cgctcaaacc gaacgatgtg agaatctcgg tcaggatcat
     7801 ccgattcacg gcgttatcct cgacgtacaa caccgttttt ccgacgaagg aggaggaacc
     7861 cgtctctagg gctttggact ccaccgtgag gggggaggac gcgggcggca ggcggagtgt
     7921 gaacgaaaaa gtcgtgccaa aacccagacg gctttcgaag gagatccgcc cgcccatctt
     7981 ttccacgagg tctttggcga tagaaaggcc caatcccgtg ccgccaaact ggcgggcgac
     8041 agccgcatcc ccttggtaga aagcctcaaa caggtgtttt cctttctcct ccgaaatccc
     8101 catgcccgta tccgacacct cgaaaagcat cgtctgtttt ccgtcctggg cattctcctt
     8161 gggagagccc cgggcggtca gtatgatgtg ccctccggaa ggggtgaatt tgacggcatt
     8221 actgacgagg ttgataaata tctgtttgat ccgcatcggg tccccgcggc attcggtggg
     8281 caaagagtcg tcgattttca gcaccatcgt caaccctttt tctgtggcga gagaatcgaa
     8341 gaggtagaag acgtcttgga tgaccaatct gaggttgaaa tccaccgatt ccagaagcaa
     8401 tttgtccgat tcgatcttcg aaaagtccaa gatattgttg atgatgccgc ttagagttct
     8461 catagcggat tccaagtgtt ccatctgctg cttctgattt gagtccaagc gggactttgc
     8521 gagaaggaag acgatgccgg agatgccttg cagcgggttt cgcaactcgt ggctcatcgc
     8581 cgagagaaag gcgcttttcg ctcgattcgc cgcttccgct tgattccgag cgctttccaa
     8641 ttgacgcaga acgagcttct gcgcggtcac atcttgaaaa ataacgatct tcccattcaa
     8701 cctgccccga aaatcagaaa agggggttat acgtaactcc aagacgcgtt ctttaccgtc
     8761 tcgagtggtc agctcgatgg ttttggttac gtttacggca tttgccaaga gctctgtcag
     8821 ccgtggccag ttacggaggg ctttttcgca cacctccccc cgattttcca gcgacaggcc
     8881 cagccactct tcaagcgcgg gattccatcc gaccacccgc tggtggcggt ccagcgcgag
     8941 aaccccatct tcgaaggtat agatgacacg ttcccaggcg atgggtataa ggtccaggaa
     9001 gcgaaatttg aaactggcta agacgaggag gaacgtcatt gcggtaaaag aaagcggggt
     9061 agggtcgaat cccccaatta aactaggaac gaacgcatag acgatgttcg ccacaagcgg
     9121 gcaaaaggag gccaacaaca acactttgga ggctgtgggg atgccggtgt ctttcgaaaa
     9181 aaagaagtgg cggctcaaca agaaaatggc cgcaagggcc atcgagtaat tgtaaatgac
     9241 ggaaaaccaa aagaaaatgc cgtgctcgaa aacgccggcg actccgcctg gtacagccct
     9301 caaggtgata ttcggccaca agaggcgatg caatgggttc gtgaccgcga gaacaaggat
     9361 gatcgcagga aag
//
LOCUS       DAOPXN010000081         9098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7647_length_9098_cov_5.077740, whole genome shotgun sequence.
ACCESSION   DAOPXN010000081 DAOPXN010000000
VERSION     DAOPXN010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9098
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_9098_cov_5.077740"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PKM49_05850"
     CDS             complement(<1..897)
                     /locus_tag="PKM49_05850"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HNY82373.1"
                     /translation="MDPVAEKEIPLLEVKGISKEFSGVRVLDRVSFSLSRGEIFGIIG
                     ENGAGKSTLIKIISGIYHPTEGSLLFEGKSVTVRDAETAHKMGINLIPQEFNLIPEMT
                     VSENIFLGKELRNRWFLDKRAMIEKTRSLLEHLRSDIAADARIESLSVAQKQMVEFAK
                     ALASESKLLILDEPTTVLTRKEIDILFEIMRQAKQSGVAMIYISHKLKEVKEICDKVM
                     VLRDGKLISIESSEHLGVHDMATKMVGRELSQIFPPKAKPQEDTVLQVRNLSVPNCLE
                     GVSFDLKRGEILGFAGLVGAGRT"
     gene            complement(912..1898)
                     /locus_tag="PKM49_05855"
     CDS             complement(912..1898)
                     /locus_tag="PKM49_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007038251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HNY82374.1"
                     /translation="MRKVTLFTGQWADLTFEKLCEKVSEWGYDGLEVACWGDHLNVKK
                     AASDPSYVENRRSILERFNLGCWAIGAHLPGQCVGDDWDERLDNFAPDAVKGKPEAIR
                     NWAIEEMKAAALAAKNLGVKVVTGFLGSPIWKYFDSFPQTTEEMIKKGFRKIYDLWSP
                     IFDEFDRCGVLFALEVHPTEIAYDFYTTQRLFDVFSDRKTLGLNFDPSHLLWQGVTPH
                     LFLREFQSKIYHVHMKDVALNADGKAGILGSHLPFGDARRGWNFRSLGHGHVNFEEII
                     RALNDMQYTGPLSVEWEDSGMDREYGARQALGFVRSLDFEPSTIAFDDALKK"
     gene            complement(1902..3095)
                     /locus_tag="PKM49_05860"
     CDS             complement(1902..3095)
                     /locus_tag="PKM49_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY82375.1"
                     /translation="MIESRNIGGQSRLTYGQIGGGPGSFIGPVHRAAIAMNNQARIVS
                     GCFSQNLEKSKETARELDIDLNRVYSSYEEMAQKEALRKDRMDFVVVSGPNHMHYETA
                     KSFLEKDFHVVCEKPLSLDLTQAKTLLKLSKERKRLFMVTYTYTGYPMVREARHLVRS
                     GKLGEIRLVVAEYAQDWLAEKAEESGNRQAQWRTDPRYAGISCCVGDIGTHIENMVHY
                     ITGLEIKRLSARLDTFVKGRSLDDNAFILLTYENGASGSYWASQVAVGKENGLVVRIY
                     GTKGAIEWEQENPNELRLSLLGAPQAILRRGNGYLSAPAKSLTRLPSGHPEGYFEAFS
                     NLYRNFCVALTRFEETGTLEPRGEAEDFTDVLDGARGIGFVEDCVKSSRLEGAWVDAT
                     KISEG"
     gene            3386..4021
                     /locus_tag="PKM49_05865"
     CDS             3386..4021
                     /locus_tag="PKM49_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY82376.1"
                     /translation="MPVFTRNRTKKWTDKELLENRRQIGDKVFWEEIYRRYASYLISI
                     ITFKYPDFRDVAEDITQETFLRLVFEDLDKVQNLKALLATTAAHICIDKHRLRSAKKR
                     ADTNLVPLDAELGNEDGEDSFLEITENVDSDNPEEIALNEIKVDEYRRVLSQLPEECQ
                     KLLMYSAEGMKYKEIANLMDLPIGTVGTKLLRCRERLKEIIEKENKEIEEG"
     gene            4027..5769
                     /locus_tag="PKM49_05870"
     CDS             4027..5769
                     /locus_tag="PKM49_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNY82377.1"
                     /translation="MDKTEFVYEGNSSKAVDVLLLTGDAFVDHPAYGVAIVARHLQAM
                     GFRVGILSHTHISAPSLHAFGKPRLFVGITSGNLDSMVSNYTASQKKRRTDDLSFSDS
                     GEKRPDRAVIVYANLVKRVWKDVPIVLGGIEASLRRFGHYDWWQDKVRHSILLDSKAD
                     FIFYGMAERTLTEAAGLFAFPDWRERVSRLRGVAYTLTNRQELPSEGIRIPSFEEVSS
                     SKEAYSEAFRLFYQETDPIRGKVIYQTDGTRAVVQNLPSFPLETAELDRIYGYPYTRE
                     LPEFYRTQGLRVKGVETVRFSITGHRGCYGSCAFCAIGVHQGRTVTWRSETSIMNETK
                     IIASHKEFKGYISDVGGPTANMYGYECEKKIAEGACKDRLCLHPEPCPSLNPNHETYL
                     RLLNRLKTIPGVKRVFISSGIRPDLVLADSRNGDRFLNALVESNVSGQLKIAPEHVSA
                     GVLREMRKYPHTVFKEFTRRYALEAKAQRKDIYLVPYLLVAHPGEGVEENEELRSFVQ
                     TELGFHPEQIQIFTPTPSTLATTVYHTGFDPWTKEPVFSEKSLTNRNRMKKRILTIRE
                     GKAKHGDYEGACEE"
     gene            5738..6781
                     /gene="tdh"
                     /locus_tag="PKM49_05875"
     CDS             5738..6781
                     /gene="tdh"
                     /locus_tag="PKM49_05875"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HNY82378.1"
                     /translation="MATMKALVKSEQARGLSLRRVEIPTITEDHEVLVKVKYASICGT
                     DLHIYQWNEWAKNRINTPQISGHEFSGEVIGVGKAVKGVQIGDFVSAETHIPCGYCRL
                     CRTGNMHICRNMKILGVDTDGVFAEYAKVPEVVLWKNSPALSQHFASVQEPLGNAVFT
                     VTTGGVAGRSVLITGAGPIGLMAIQVAKALGAGPIIVSEVKDYRLDMAAKNGADYVFN
                     PTKEDVFRKVKDITNEEGVEVALEMSGNEKALALCIDALLPSGHISALGVFDKPFPID
                     INKVIFKNITLYGITGRKMFETWYIASSLLLNKRVDLNKIVTHLLPLEEWEKGFLLME
                     QGNCGKVVLKIGE"
     gene            6856..8472
                     /locus_tag="PKM49_05880"
     CDS             6856..8472
                     /locus_tag="PKM49_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNY82379.1"
                     /translation="MLLGRAYQLQVVHYEENAERLDRMRLRRDLVPALRGNIYDRQGT
                     LLANTTFRRVVDLEATFRGVYGRERTDLIRSVAAQCGVTEIEVRDAMNKAMDLPLRAD
                     LPSANVNLSVKYLLTRNYSFQGSLSHIVGYVNKASVGIAGIEYTLNHWLSGEPGQYRI
                     KVDSRTRTLTQRWIQKPKPGKNLTLSIHYGFQKAIEEIAKPLENPYTIIVSNPKTGEI
                     LSMVSSPYYESARMVQPFTQVEWDRLQNDPNRIFINRAIQTRYNPGSLIKPWVALCSM
                     EQSSAFFQTTIVNREICKGNFPVLTTFGDTVHYRDWVATGHGEVNLFFALQKSCNTFF
                     YNLGSLLGIERMYDFSSVSRLTSKSGIELPNEVAGTFPSADWKKNTLHERWYYGDTLL
                     TAIGQSYVQLTPLEMCKLFELIANEGVVARTTLIKNGQAPTTRVLDYPKAYWTFLKEA
                     LGAVISRPGGTAYSVFLGAPYALHLAGKTGTAETGLEGVYHSWFACFYPKEDPEVTVL
                     VFVEKGGFGSATAAPVARQVFDAYLEYTFD"
     gene            8517..8720
                     /locus_tag="PKM49_05885"
     CDS             8517..8720
                     /locus_tag="PKM49_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNY82380.1"
                     /translation="MADQSEVKVPDMSCNHCVKRISNALKEGGIETFHIDLEKKEVRV
                     NTTQLREVLHILEKIDYPGSVSK"
     gene            complement(8754..>9098)
                     /locus_tag="PKM49_05890"
     CDS             complement(8754..>9098)
                     /locus_tag="PKM49_05890"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNY82381.1"
                     /translation="SINMLSEALSYLTADVAVGKPLVVGDKTLIPLFKATTGFGGGGG
                     GPNGVVFGSGVGGSLEFLPFAIVVVSGDKVNIIPVLNEKPFFEQLVNGLPQIMPIVMQ
                     LISMFGGSPGGN"
BASE COUNT         2319 a         2380 c         2294 g         2105 t
ORIGIN      
        1 ggttctacct gcgcccacga ggcccgcgaa ccccaggatt tctcctcttt tcaagtcgaa
       61 agagacgccc tccaaacagt tcgggaccga taggttccgg acttgtaaca cggtgtcttc
      121 ctgcggcttg gctttaggcg gaaaaatctg agaaagctct ctacccacca tcttggtagc
      181 catatcgtgg acgccgagat gctcggagga ctctatagag atgagctttc cgtcgcgcaa
      241 gaccatcacc ttatcgcaaa tctctttaac ctctttcagt ttgtgggaga tatagatcat
      301 cgcgacgccg gattgtttcg cttgtctcat aatttcaaac aagatgtcga tctctttacg
      361 cgtaagaacg gtggtcggct catccaatat caggagcttg gactccgagg ctaatgcttt
      421 cgcaaactct accatctgtt tctgcgctac gctcagcgat tcgatcctcg cgtccgccgc
      481 gatatccgag cgtaggtgtt ccaagaggga ccgagtcttt tcgatcatcg cacgtttgtc
      541 taaaaaccat cggttgcgca gctctttgcc caaaaaaata ttctccgaaa cggtcatttc
      601 ggggatgagg ttgaattcct gcggtataag gttgatcccc atcttgtggg cggtttcggc
      661 atcgcgcacc gtcacgctct ttccttcgaa gagtaggcta ccttccgtcg ggtgatagat
      721 tccactgatg atcttaatca atgtagactt gccggctccg ttttccccta tgatcccgaa
      781 tatctctcct ctagatagag aaaaggagac gcgatcgagc acgcgtaccc cggagaattc
      841 tttcgaaata cctttcactt ctaatagggg gatttctttt tcagccacag gatccatagg
      901 gcgctccaaa actatttttt tagggcgtcg tcgaaagcga tggtcgaagg ttcgaagtct
      961 aaggatcgga caaaaccaag cgcctgcctt gccccatatt cgcgatccat accactgtct
     1021 tcccattcga cagaaagcgg gcccgtgtat tgcatatcgt tcagcgcgcg tatgatctcc
     1081 tcaaaattga catgaccgtg acccaaagat cggaaattcc accctctcct cgcgtcgcca
     1141 aagggcaaat gcgacccaag aatgccggcc ttgccgtccg cgttcaaagc cacgtccttc
     1201 atatgtacgt ggtagatttt cgactgaaac tcacggagaa acagatgagg ggtcacccct
     1261 tgccataaca gatgcgaagg atcgaagttt agcccgaggg tttttcggtc ggagaaaacg
     1321 tcgaaaagac gttgggttgt ataaaaatcg taggcgattt cggtgggatg cacttcaagg
     1381 gcgaataaaa ccccacaccg atcgaattcg tcgaagatcg ggctccagag atcgtaaatc
     1441 tttcgaaatc ccttctttat catttcttcg gtcgtttggg ggaaggaatc gaaatatttc
     1501 cagattggag agccgagaaa cccggtcact actttgacac ccaagttttt cgccgctagg
     1561 gcggccgctt tcatctcctc gatagcccag tttcgaatcg cttccggctt acctttcaca
     1621 gcatccggcg cgaagttgtc cagcctttcg tcccaatcgt ctcccacgca ttgccctgga
     1681 aggtgggcac cgatagccca acaccctaag ttgaatcgct caaggataga ccgacggttt
     1741 tctacgtaag agggatcgct cgcggccttt tttacgttca aatgatcccc ccaacacgcg
     1801 acttccaagc cgtcataacc ccattcggag actttctcgc acagtttttc aaaagtaaga
     1861 tcagcccatt ggcccgtgaa aagtgtcact ttcctcatac cttatccctc cgagattttc
     1921 gtggcgtcaa cccaagcgcc ttcaaggcga ctactcttga cgcagtcctc tacgaaaccg
     1981 attccgcggg ctccgtccaa gacgtccgtg aagtcctccg cttctccgcg cggctccagg
     2041 gttcccgttt cctcgaagcg cgtcaacgcg acgcagaagt tccgataaag attggagaag
     2101 gcttcaaagt aaccctccgg atggcctgaa ggcaagcgcg tcaaactctt cgctggcgcg
     2161 gacagataac cgttacctct gcggaggatc gcttgcggcg ctcccaacaa cgagaggcga
     2221 agttcgttcg gattctcctg ctcccactcg atcgcgcctt tcgtgccata gatcctgacg
     2281 actaacccgt tttctttgcc gacggctact tgggaagccc agtaactgcc ggacgcgccg
     2341 ttttcgtaag tcaacaggat gaaggcgttg tcatccaaag agcgtccctt cacaaaggtg
     2401 tccaagcgag cggataaccg tttaatctcc aaccctgtga tgtaatgaac catattttcg
     2461 atatgcgtgc cgatgtctcc cacacaacaa gaaataccgg catatcgagg atcagtcctc
     2521 cactgagctt gccgatttcc ggactcttcg gctttttcag ctagccaatc ttgcgcgtat
     2581 tcggcgacga ccaggcggat ctctcccaat ttaccactac gaaccaaatg tctggcttct
     2641 cgtaccatcg ggtaaccggt gtaggtgtag gtcaccataa aaagccgttt tcgctctttg
     2701 ctcaacttta aaagcgtttt tgcttgagtc aggtctaagg acagaggttt ttcacatact
     2761 acgtggaagt ccttttcaag aaaggatttc gcagtctcgt aatgcatatg atttggaccc
     2821 gacaccacca caaaatccat tctgtctttc cgtagcgctt ctttttgggc catttcttca
     2881 taactagagt agactcggtt aagatcgatg tctaattctc gtgccgtctc tttcgatttt
     2941 tccaagttct gtgaaaagca ccccgagacg atccttgcct gattgttcat cgcaatggcg
     3001 gctcgatgga ccggcccgat gaaagagcca ggacccccgc cgatctgccc gtaggtcaat
     3061 ctcgattggc caccgatgtt tctcgattcg atcaccaaaa accacctccc cttcgattgg
     3121 ctgattcggt ttaattgatt gtatcaataa actatttgat tcacaagagc gttttcgcct
     3181 tctcgccaga aaatcgagcg ccctagagcg gtttgtgtcg gaagagcttc gtttttcttt
     3241 gtttttttcg taatttctta cttttccgaa aatggaccac ccgtggtcgg tgaaatacga
     3301 atgcttaaga ggggaatgac cccggatgcc ggacgattag gaaatatggt agaataaatc
     3361 gttgtcacag aaaggaatcg aaaagatgcc tgttttcacg cggaatcgaa cgaaaaaatg
     3421 gacggataaa gagctgctcg aaaatcggag gcaaatcggc gataaggtct tttgggaaga
     3481 gatctatcgg cgctacgctt cgtatctcat ttctattatc acctttaaat atcccgactt
     3541 tcgagacgtc gcggaagaca tcacacagga gacctttcta cgcctcgttt tcgaagattt
     3601 ggacaaggtg cagaacttga aagcgctgtt agccacgacg gccgcgcaca tttgcatcga
     3661 caaacaccga ctccggagcg caaaaaaacg cgcggatacc aacctcgtcc cgttagacgc
     3721 tgaacttgga aacgaggatg gagaagactc ctttctggag ataaccgaaa acgtggattc
     3781 ggacaatcca gaagagatcg cactaaacga aatcaaagtg gacgagtatc gtcgggttct
     3841 ctcccaattg ccggaagagt gccaaaaact cttgatgtac tcggcggagg ggatgaagta
     3901 caaagagatc gccaacctga tggacctgcc gatcggcacc gtggggacga agctgttgag
     3961 gtgcagagag cgactgaaag agatcatcga aaaagagaac aaagaaatcg aagaaggcta
     4021 gaaaccgtgg ataaaacgga attcgtatac gaaggaaact cttcgaaagc ggtggacgta
     4081 ttgttgttga ccggagacgc ttttgtggat catccggcct acggtgtcgc gatcgtggcg
     4141 agacatcttc aggcgatggg gtttcgagtg gggattctca gtcacaccca tatcagcgcg
     4201 ccctcccttc acgcattcgg aaagccgcgg ttgttcgtcg gaatcacctc gggaaatctc
     4261 gattcaatgg tgtccaacta caccgcctct caaaagaagc gcagaacgga cgacctctct
     4321 tttagcgatt caggggagaa gcgcccggat cgggccgtca tcgtatacgc caacctcgtc
     4381 aaacgggttt ggaaagacgt tcctatcgtt ttaggcggca tcgaggccag tcttcggcgg
     4441 tttggccact acgactggtg gcaagataag gtgcggcatt ccatacttct cgacagtaaa
     4501 gcggacttca ttttttacgg aatggccgag cgaaccttga cggaggccgc cgggctgttc
     4561 gcctttccag attggagaga gagggtctct cgcctcagag gagtcgcgta tacgttgacc
     4621 aatcgacagg agcttccctc ggaggggatt cggatccctt ctttcgaaga ggtctcctct
     4681 tcaaaagaag cgtattccga agctttccgc ctattctatc aggagaccga ccccatccgc
     4741 ggaaaagtta tctaccaaac ggacggaacg cgcgcggtcg ttcaaaacct tccgtctttc
     4801 cccctggaga cagcggaact ggatagaatc tacggatacc cctacacacg agagctcccc
     4861 gagttttatc gaacccaagg actccgtgtc aagggggtag aaacggtccg tttttctatc
     4921 accggccatc gcgggtgcta cggcagttgc gctttttgtg cgataggagt ccaccaagga
     4981 agaaccgtga cctggcgaag cgagacctcg attatgaacg aaacgaagat catcgcttct
     5041 cacaaggagt ttaaggggta catctccgat gtgggcgggc cgacagcaaa tatgtacggg
     5101 tatgagtgcg agaaaaaaat cgcagaaggg gcgtgtaaag accgcttgtg ccttcacccc
     5161 gaaccctgcc cttccttgaa cccgaaccat gagacgtacc ttcggcttct aaaccgtttg
     5221 aaaacgatac ccggggtcaa gagggtcttc atctcttcgg ggatacgccc cgatttggtg
     5281 ttggccgatt cccgcaacgg tgatcgattt cttaacgccc tcgttgagag caacgtttcc
     5341 ggtcagctca agatcgcacc ggaacacgtg agcgcggggg tcctgcgcga gatgcggaaa
     5401 tacccgcaca cggttttcaa agagtttacg cgccggtatg cacttgaggc caaagctcag
     5461 aggaaagaca tctaccttgt tccctacctg ctcgtcgcgc atccgggaga aggcgtggag
     5521 gaaaacgagg agctacggtc cttcgttcag accgaactgg gattccatcc agaacaaatc
     5581 cagatattta cgcctacgcc ctcgacgctc gccaccaccg tctaccacac ggggtttgac
     5641 ccgtggacga aagaacccgt tttctctgaa aaaagcctga cgaaccgcaa tcgtatgaaa
     5701 aaacgcatcc taactatccg ggagggaaaa gcaaaacatg gcgactatga aggcgcttgt
     5761 gaagagtgaa caggccagag gtttatcttt acgcagagtg gaaatcccca ccatcaccga
     5821 ggatcacgag gtgttggtca aggtgaagta cgcttcgata tgcgggaccg atctgcacat
     5881 ttatcaatgg aacgaatggg caaaaaaccg aataaacacc ccgcaaatct ccggacacga
     5941 gttctccggt gaagttattg gggtcgggaa agccgtgaaa ggggtgcaaa tcggagattt
     6001 cgtctcggcg gagacgcata tcccttgcgg ctactgccgc ttatgccgca ccggcaatat
     6061 gcacatatgt agaaatatga agatactcgg agtagatacc gacggtgtct tcgccgaata
     6121 cgcgaaggtc ccggaagtgg tattatggaa aaactcacct gcgctctcgc agcacttcgc
     6181 ttccgtgcaa gagccgctcg gcaatgccgt attcaccgta accacgggag gcgtcgcggg
     6241 aagaagcgtc ctcatcacgg gcgcggggcc gatcggtctt atggcgattc aagtggcaaa
     6301 agctctgggc gcagggccga ttatcgtttc agaagtcaaa gactatagac tcgatatggc
     6361 tgctaaaaac ggcgcggatt acgtttttaa tccgaccaaa gaggatgtct tccgaaaagt
     6421 aaaggatata acgaacgaag agggggtaga ggtcgccctc gaaatgtctg ggaacgagaa
     6481 agcgttagcg ctgtgcatag acgcgctctt gccgtccggc cacatctccg ctctgggcgt
     6541 atttgacaaa ccctttccta ttgatatcaa caaggtgatc tttaaaaata tcacactcta
     6601 tgggatcacg ggcagaaaga tgtttgagac ctggtacatc gcctcgagcc tattgctcaa
     6661 caagagagtc gatctgaaca agatcgtcac acatcttctc ccgttagaag aatgggaaaa
     6721 aggctttcta ttaatggaac aaggaaactg tggaaaggtc gttctaaaaa tcggagaata
     6781 acccttaaaa agctgccgga taggagagaa aagaggatac gtttcctttt ctactttctc
     6841 ctattatcgg taatagtgct actcggacgg gcctaccaac tacaagtcgt ccactacgag
     6901 gagaacgcgg agcgtttgga tcgtatgcgc ttacgtcggg atcttgttcc ggcgctacgc
     6961 ggcaacatct atgaccggca gggaacgttg ctggccaaca ccaccttccg gcgagtggtg
     7021 gacctcgaag cgacctttcg cggggtttac ggtcgagaac gtacggattt gatccgaagc
     7081 gtcgccgcgc aatgcggtgt gacagagata gaagtccgag acgcgatgaa caaagcgatg
     7141 gacctccctc tccgggcgga tttgccttcc gctaacgtca acctcagcgt gaaataccta
     7201 ctaacgcgta actactcctt tcaagggtca ctgagccaca tcgtcggtta tgtgaacaaa
     7261 gcgtcggtag ggatcgcagg gatcgaatac accctcaacc attggttgtc aggggaaccc
     7321 gggcaatatc gaatcaaagt ggattcgcga accagaacgc tgacccagcg atggattcag
     7381 aaaccgaaac ccggaaaaaa tctcacgtta tcaatccatt atggctttca aaaagctata
     7441 gaagagattg caaagccatt ggaaaacccg tataccatca ttgtgtcgaa ccccaaaacg
     7501 ggagagatac tttctatggt ctcctctccc tattatgaaa gcgccaggat ggttcaaccg
     7561 ttcacccaag tcgaatggga cagactgcaa aacgatccca accgtatctt cataaaccgc
     7621 gctatacaaa cgcgttataa cccaggcagt ctgatcaagc cttgggtcgc cctctgctct
     7681 atggagcagt cgagcgcgtt ctttcagacg acgatcgtga atcgagagat ctgcaagggc
     7741 aatttcccgg ttttaaccac cttcggtgat accgtccact atcgtgattg ggtggccacg
     7801 ggacacggag aagtcaacct ctttttcgcc ctacagaaat cctgcaacac ctttttttac
     7861 aacttaggct ctctattggg aatagaaagg atgtatgact tttctagcgt gagccggcta
     7921 acgtcaaaat cggggataga gcttcctaac gaagtggcag gaacctttcc ctccgcggac
     7981 tggaaaaaga acacgttaca cgaacgctgg tattatggag acaccttgct aaccgcaatc
     8041 ggtcaaagct atgtgcagct cactccgctc gagatgtgca agttgttcga attgatcgcg
     8101 aacgaaggag tcgtggcgcg aacgaccctt ataaaaaacg gtcaagcgcc gacaacccgt
     8161 gtgctggact atcccaaagc ctactggact tttttgaaag aggccttggg agccgtcata
     8221 tcgagaccgg gcgggaccgc ctacagcgtt tttctcgggg ctccgtacgc gctccatctg
     8281 gccgggaaga ccggaacagc ggagaccggg ttggaaggcg tctaccattc ttggttcgcg
     8341 tgtttttacc cgaaagaaga tcccgaagtc accgttttgg ttttcgtcga aaagggcgga
     8401 ttcggcagcg cgacggccgc gccagtagcc cgccaagttt ttgacgctta tctagaatac
     8461 accttcgact aaaagcgtct tgatacagaa agagcggcga gaaaggaggg gagtctatgg
     8521 ccgatcaaag cgaagtgaag gttccggata tgtcttgcaa ccactgtgtg aagcggatat
     8581 cgaatgcatt aaaagagggg ggaatcgaaa cgtttcatat agacttagaa aagaaagagg
     8641 tccgcgtgaa cacgacacag cttcgggaag ttttacatat actcgagaaa atcgactacc
     8701 cgggcagcgt ctcgaaataa aaaagggctt tcgccctttt ttttatttac cgttcaatta
     8761 cccccggggg agccgccgaa catagagatc agctgcatca cgatcggcat aatctggggc
     8821 aagccgttga ccaactgttc gaagaacggt ttttcgttga gaacaggtat gatattcacc
     8881 ttgtctcccg aaacaaccac gatcgcaaaa ggcaagaatt ccaaagaacc tccgactcct
     8941 gacccgaaaa cgacgccgtt cggcccgcct cctcctccac cgaatcccgt ggtggcttta
     9001 aacaggggga tcaaggtctt atcgccgaca actaacggtt ttccgacagc cacatccgca
     9061 gtcaggtaag agagcgcttc ggatagcata ttgatgct
//
LOCUS       DAOPXN010000082         8972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7785_length_8972_cov_5.463833, whole genome shotgun sequence.
ACCESSION   DAOPXN010000082 DAOPXN010000000
VERSION     DAOPXN010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8972
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7785_length_8972_cov_5.463833"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            956..1426
                     /locus_tag="PKM49_05895"
     CDS             956..1426
                     /locus_tag="PKM49_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNY82382.1"
                     /translation="MQKERKLTSSLEEYLKVIYEITQTEPVARVKTIAKHMGVSLPSV
                     TNALKRLDVLGYVRYEKYGLIMLSEKGIERAQKLEKTCCILRTLLVDFIGVPESDLSK
                     ITCRIDHYFDEETQHRMQELLCILRKAKDGGTEYRNELELFFKKRSSEISSTTE"
     gene            1470..2366
                     /locus_tag="PKM49_05900"
     CDS             1470..2366
                     /locus_tag="PKM49_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNY82383.1"
                     /translation="MVGILGDVMIDQLASIRDYPEEGSETIIRSLTPRLGGSACNTAM
                     VLSDLVGRCLLFSQIGDDEHGHLAVATLMKKGVIPDFVSTRPGMPTGMIIIMLSPDGS
                     RTMFSHRPSHPLPDDEGLEDRFISRIDFLHLSGYVLLEERDRAICHRILDKAAKKGLP
                     ISLDPGISPVKKVPEAVLAALDWISYFIPNEKELLILTQSPDLFSAIDTLGAQKPVLV
                     IKRGEMGCLIVNRDGSLPVPSEKAVVTTNTTGAGDGFNAGFIYGLLQRWPLERCARLG
                     NQMGRQVVSSPLGIPGTSFYLT"
     gene            2737..3801
                     /gene="mtnA"
                     /locus_tag="PKM49_05905"
     CDS             2737..3801
                     /gene="mtnA"
                     /locus_tag="PKM49_05905"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNY82384.1"
                     /translation="MEALLPLRFTGFSVLVLDQRLLPREEKYTEYFDLEGLYESIRSM
                     ELRGAPLIGIAAAYAMFLGVKNRSFPNQAVLLETVKKNAERLNQARPTAKNLFWATAR
                     LVRLVERNIGLRVDEIIDRMRAEAVRINEEDVEICRSIGEHFLTVLKPGMRLLTHCNA
                     GALATYRYGTATAPMYLGQERGWNFRVFVDETRPYNQGARLTAYELHRAGIDATLICD
                     NMAAMVMSKGWVDAILVGSDRVAANGDAANKIGTLNLSILARHFGIPFYVATPTPTLD
                     LELPDGNHIPIEERDGEEITHFLGVRVAPEGIKVFNPAFDVSPHENITGIVTEKGIAY
                     PPFELSLKALAEKTGPSGNG"
     gene            3805..4611
                     /locus_tag="PKM49_05910"
     CDS             3805..4611
                     /locus_tag="PKM49_05910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNY82385.1"
                     /translation="MAGNLVDTHTHLWDKRFDRDRQHLIRSLHENGIAWILEVGVDLP
                     TSQKSLTLAETYPQVYASVGTHPHDVKDLDQDRLVALKELARNPRCVAIGEIGLDFYR
                     DLSPRPLQIKWFKEQLEWALSIQKPVILHVRQAYEEALAILEGYGSFPAGGVAHCFQS
                     DMRTAERFVALGFHIGIGGPITYSSKDPNAVETLREVARNLPFERLLGETDCPYLTPE
                     PVRGKRNEPAFVKYAIAGIARALERSFEETAFGLRKNAEGLFLVDNVTDR"
     gene            4840..5799
                     /gene="hflK"
                     /locus_tag="PKM49_05915"
     CDS             4840..5799
                     /gene="hflK"
                     /locus_tag="PKM49_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNY82386.1"
                     /translation="MADTDKVRIPKWTFTLVMVASIVGILVIYFSTGFYNVAPSEVGL
                     VKRFGKYVRTDQPGINYHLPAPFDSVTVVDVLSIRKIEIGFRTVAANRYASVVSEALM
                     MTSDNNFASVECVIQYRIKDAYSFAFNIHNPEEIVKALSESVIRERVALRTVDEVLTS
                     DRDLIAMEAKDRLQELLDRFQIGVSLENVRLQEVNPPTEVLAAFDDVNSAIQDKEKTI
                     NTALRYYNDLVPKAQGEAGKVLLEAEGYRDIRVLAAQGEVARFLQVLEKYEASPEITR
                     QRLYIETMEKVLPNFKTLILPGDSGNTLKLLDIQALMNGGGRQ"
     gene            5796..6656
                     /gene="hflC"
                     /locus_tag="PKM49_05920"
     CDS             5796..6656
                     /gene="hflC"
                     /locus_tag="PKM49_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HNY82387.1"
                     /translation="MKTLWWFLGIFLAFFILFAMVTYTVDETQLAIRIRFGKILDVHD
                     TAGIHFKAPFIDRIEKRDKRLMLYDIPPESVLTADKKRLEVDSFILWRISDAKAFLET
                     LNNTVSALNQIDDIVYANIRDSFAKLTIPDIISEERRAFLGSITVQTSENLKTFGVQV
                     ADVNVKRTDLPETNAKAVFERMKSERYQAAAKIRAEGEKEYKEIVAKAEKESSVLIAE
                     ANKRAEEIRGEGDASALEVYASAFSKDPEFYRFWRSLSAYENVFQKEAVVLLDERVDF
                     LQYLFSHKAE"
     gene            6663..7370
                     /locus_tag="PKM49_05925"
     CDS             6663..7370
                     /locus_tag="PKM49_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HNY82388.1"
                     /translation="MGVGPSADSYLSVRASAFDRGETAEESTETTIVIQRSRFIGCAA
                     RVLSKDDVTDFIKSRKARHPQANHHVWAYVIDVKGGSQHFSDDGEPGGTAGAPVFGVL
                     SKHRLERTALVVTRYFGGIKLGISGLIEAYRRSAEETIQAVGIARYRLYRRYAFALAY
                     PDWKGFQRFLSALGVEENGIEVRYAEKVEVLVTVLVESAPRLREELDRFIAMRKIIGY
                     TESDPAFVPDLSETDTL"
     gene            7386..>8972
                     /locus_tag="PKM49_05930"
     CDS             7386..>8972
                     /locus_tag="PKM49_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82389.1"
                     /translation="MSVWEALVFSISLITSNLLILNGIAKFDLKGKTPFTFLLMAFAT
                     STGYGLLRYVTIILYDDFYLVTNILFFLNNLWLLVFFRNAVVQYNDRIPIGRYFVLSI
                     VCFLLPISIGLFFNPINNFETLMVQMSYMLGIIPMVVYSSMLAGVSVGSAFWGIIIAC
                     FLFSVDFFLRFGFFHILQTEFQEVISETFRVVSILLISIFGHYGQPIPPGKIREVIPG
                     TQRLNNIFFALIAFFPVMIFYTVFWQVNLNDRKMQQKEIELSKEVFMISSRMLEEKID
                     TYSRIVNEVKNSANLATAQWKLKTFLFTDPEIDVAQLLTSYPIMYKDEEVIFEKGRVT
                     FYTRTEQGWGIKLSVNMEVMYAQFPVNESQRFYAFLNDTTFLYPPGQMIEANALFSSI
                     GKRDFVFVNTINLYKKPFSTYLIFTTSPITVNIYTLAFMLGAIFILVLVWWFYGFYVH
                     RWEKTAKEKYFSLNQAKEEYGEQIGRLESSLKLISGDLQITADKLDQFSRTVYQLTDS
                     FSGFDFQKGPSFELKQLYDKL"
BASE COUNT         2255 a         2210 c         2441 g         2066 t
ORIGIN      
        1 taagggtcta cagaattatt acgatagaag atgttaatta agtacgtctt gtaaaatgtt
       61 gaagagccaa ggaaaaatgc ctagtttaaa aaacagaaga tttagcgcgc acagagggta
      121 cagagaagga atgcctggat taaaaacaaa agatctcgtt cgaacagaga gcacaggggg
      181 catggagagg acacaaagga aaaatgccta gtttaacaaa cagaagattt agcgcgcacg
      241 gagcgtacag agaaaaaatg cctcgattaa aaagcaaagg atctagttcg cacagagagc
      301 acagagggca cggagaggac acgaaggaaa aacgcctagt ttaaaaaaca gaagatttag
      361 ttcgcacgga gggtacaaag aaagaatgac ttgattaaaa agcaaaagat ctagctcgca
      421 cagagagcac ggagagaggt tgaagaagac ttgccgcaca gaagcgcgac gttcccaggc
      481 tccggaaaag ttcgcctctc cactttaaac gctcataaga atgtaagcgc actgataaca
      541 cgaattggtg gtataatatt gtttgaggat agcgaaatcg taattgcttt cctatgtttt
      601 gtagaaattt acctcttgta tttcgcgttt aaatcgtata aaatacttgc agcagtcaac
      661 ttacttcatt tttaactgtg taggtgcggg gatgctgtac cgggtgaact acggagtcac
      721 gtatggggct gtttgacgtt ctcaaggaga acaggccgaa caaaagttcg gcgttctcgg
      781 gaaataaaga catgccgaac tgaagttcgg ggctcccagg ggtgcgaagt tcccagaggt
      841 tcggagttcc cggagaaaaa agatatgctg aattaaagtt cggcgctccc aggggtgcgg
      901 agttctcagg cgtttccaag gagaaatata tttactgtga ggtaggaggt tgaatatgca
      961 aaaggaaaga aaactcactt cttctctcga agagtatttg aaggtcattt acgagattac
     1021 gcagaccgag ccggtagcgc gcgtcaagac cattgcgaaa catatggggg tgagtttgcc
     1081 cagcgtcacg aacgccctga agaggttgga tgtgttgggg tatgtgcggt atgaaaagta
     1141 cggtctgatt atgttgagcg agaaggggat cgagagagcg cagaaactgg aaaagacttg
     1201 ctgtatcctt cgcacgctgt tggtcgactt tatcggcgtt ccagaaagcg acctctcgaa
     1261 gattacctgt cgtatcgacc actatttcga tgaagagacc caacatcgga tgcaagagct
     1321 gctctgtatc cttagaaaag cgaaagacgg cggaacggaa taccgtaacg agctggagct
     1381 ctttttcaaa aagaggtcga gcgaaatttc ctcgacaacg gagtgatcca tttcgttcaa
     1441 aaattcgcta gttgctgtag gaggatgtga tggtagggat cttaggcgac gtgatgatcg
     1501 atcagctggc gtcgatccga gattacccgg aagagggctc ggagacgatc atccgttctt
     1561 tgacgccgcg tttgggcgga tcggcctgca acaccgcaat ggtgttgtcc gatctcgtcg
     1621 ggcgttgcct gctgttctcg cagatcggcg acgatgaaca cggccatttg gcggtggcaa
     1681 cgttaatgaa aaaaggggtc atcccggact tcgtttcgac acgtccgggt atgccgaccg
     1741 gtatgattat cattatgctc tcacccgacg gatcgcgcac catgtttagc caccgtccca
     1801 gtcacccatt gccagatgac gaggggttgg aagaccgttt catctcccga attgacttcc
     1861 tacacctgtc tggctatgtt ttattggagg agagagaccg cgccatctgt catcgtattt
     1921 tggataaggc ggccaaaaag gggctaccta tctccttgga tccggggatt tcaccggtaa
     1981 agaaagttcc tgaagcggtg cttgcggctt tagactggat ctcttatttt atccctaatg
     2041 agaaggagct gctcatcctg acgcaatcac cggatctatt ctccgccatt gacacactgg
     2101 gcgcccaaaa acccgttctc gtcatcaagc gcggggagat gggatgcttg atcgtcaatc
     2161 gcgacgggtc gttgccggtt ccttcggaaa aggcggtggt cacaaccaat acgacgggcg
     2221 cgggcgacgg gttcaacgcc ggtttcattt acgggcttct tcagcgttgg cctctcgaac
     2281 ggtgcgcccg tctcggaaac cagatggggc gtcaagtcgt ttcttctcct ctcggtatcc
     2341 cgggaacgtc gttctatttg acctaaacgc gaaagtccgg cgtgctcacg gtaagggaaa
     2401 gagggttcgc acgaaggtca cggagaacac agagggatat tttctgggaa cgccgcactt
     2461 cagtgcggca tgtcctttcg atttttctag ggggcgtaac aaagcttatt taaagaagtt
     2521 taaagctcaa accctgccgg actgaagttc ggcgggccca agaagaaaaa cgtgccggac
     2581 taaagtccgg cgctcccaaa agggcgtact cagtgcgcta ccaggcgctt ccgaaaatcc
     2641 cgaactcaca gaataccggc aagcgggtta tccgtgatat aattgatagc cgcaagtgaa
     2701 gacggcatcg ttgtgttgtg atgaggagga tatcaaatgg aagcgctctt gccccttcgg
     2761 ttcaccgggt tcagcgtcct cgtgttggac cagcgtctcc ttccgcgtga ggaaaaatac
     2821 accgagtatt tcgatctcga aggtctctac gaatcaatcc gttctatgga gctgagaggg
     2881 gccccattga tcgggatagc agcggcttac gcgatgtttt tgggagtgaa gaaccggagt
     2941 ttccccaatc aagccgtcct cttggaaacc gtgaaaaaaa acgccgaaag gctcaaccag
     3001 gcaagaccga cggcgaaaaa cctcttctgg gcgacggctc gactcgtccg cctggtggag
     3061 cggaatatcg gtttacgcgt agacgagatc atcgatcgaa tgcgcgccga ggccgtacgc
     3121 ataaacgaag aggatgtcga gatctgtcgg agcatcggcg agcatttcct gaccgtcctc
     3181 aagcccggta tgcgcctgtt aacgcactgc aacgcgggcg cgttggcgac gtatcgatat
     3241 ggaaccgcga ccgctccgat gtacctcggc caggagcgcg gatggaattt tcgagttttc
     3301 gtcgacgaaa cgcggcctta caatcaaggc gcgaggctga ccgcttatga actccatcgg
     3361 gccggtatcg acgcgacgtt gatttgcgac aatatggccg cgatggtgat gtccaaaggt
     3421 tgggtggatg cgatcctcgt cggcagcgac cgcgtggctg cgaacggcga cgccgcgaac
     3481 aagatcggaa ctctgaacct ctctattctt gcgcgccatt tcggtattcc tttctatgtg
     3541 gcgacgccca ccccgactct cgacctcgag ctacccgacg gaaatcacat ccccatcgaa
     3601 gagcgagatg gcgaggagat cacccatttt ttaggggtcc gtgtggcgcc cgaagggatc
     3661 aaagttttca accccgcctt cgatgtctct ccgcacgaaa acatcacggg gatcgtgacc
     3721 gagaaaggta ttgcctatcc cccgtttgaa ctcagtctaa aggcgctggc agaaaagacc
     3781 ggcccttccg gaaatggtta gcatatggcc ggtaacctcg tagatacgca cactcatcta
     3841 tgggacaaac ggttcgatag agaccgtcag cacctcatcc gttctttaca cgaaaacggg
     3901 atcgcatgga ttttggaagt cggcgtcgat ttgcccactt ctcagaaaag cttaaccctc
     3961 gccgagacct acccacaggt gtatgcgtct gtcgggacgc acccacacga cgtgaaggac
     4021 ctggatcaag atcgattagt ggcgctgaag gaactcgcgc gaaatcctcg atgcgtcgca
     4081 atcggagaga tcggcttgga tttttaccgt gatctgtctc cacgaccgct ccagatcaag
     4141 tggtttaaag aacagctgga atgggcgtta tcgatccaaa aaccggtgat cttgcacgtt
     4201 cggcaagctt atgaagaggc tcttgcgata ttggaaggat acggttcctt ccccgccggg
     4261 ggtgtcgcgc attgctttca atcggacatg cgaacggccg agcgattcgt cgcgctgggg
     4321 ttccacatag ggatcggcgg gccgatcacc tattcctcca aagacccgaa cgccgtcgaa
     4381 accctccgtg aggtggcgcg gaatttgccg ttcgagcggc ttctgggaga aaccgattgc
     4441 ccttacctaa ccccggaacc cgtgagagga aaacgaaatg aacccgcttt cgtcaaatac
     4501 gcgatcgccg ggatcgcccg agcgctagaa cgctctttcg aagaaaccgc gttcgggctt
     4561 cgcaaaaacg ccgaaggctt attcctcgtt gacaatgtga cggatagata gccttccttc
     4621 tcgaggaagg aggcgcgtca gtgacgctca aaacctaaac gcctgtaatc gcttaagata
     4681 aaaacgcgag agggcgataa aggcaagcga accgcccgcg ggtcagcggt cgagaacccg
     4741 cggtttaacc gtgctctaca ggctgcagaa tgctttcatt gcgctattca atgggattcg
     4801 ggcgcgatgg gagcccaaga aagattagga gggactcgta tggcagacac agacaaggtt
     4861 cgtataccga aatggacctt cacgctcgtt atggtggcca gtatcgttgg cattctggtc
     4921 atctattttt cgaccgggtt ttacaatgtc gccccatcgg aggttggctt ggtaaaacga
     4981 ttcgggaagt acgtgaggac cgatcagccc ggtatcaact atcatctacc ggccccattc
     5041 gattccgtca ccgtcgtgga tgtgctatcc atccggaaga tagagatcgg gtttcgaacg
     5101 gtagcggcca atcggtacgc ttcggtcgtc agcgaggcgt tgatgatgac aagcgacaac
     5161 aatttcgcca gcgtcgagtg cgtcatccaa taccgcatca aggatgctta ttccttcgcg
     5221 ttcaacatac acaatcccga agagatcgtg aaggcgcttt cggagtcagt gatccgtgaa
     5281 agggtcgcgc tccgaaccgt agacgaggtg ctcacctccg atcgcgattt gatcgcgatg
     5341 gaagcgaaag accgtctcca agagctcctg gatcgtttcc aaataggagt ttcattggaa
     5401 aacgtccggt tgcaggaagt gaatccccca accgaagtgc tggcagcctt cgatgatgtg
     5461 aacagcgcga tccaggacaa agagaaaacg atcaacacgg cgctccggta ttacaacgat
     5521 ctcgtgccga aagcgcaggg agaagccggt aaggtcctct tagaagcgga aggttaccgg
     5581 gacatacggg tgctggccgc gcaaggagaa gtggcccggt ttttgcaggt tctagaaaag
     5641 tacgaagcct ctccggagat cacccggcag cgcctgtaca tagagacgat ggaaaaggtg
     5701 ttaccgaatt tcaagacgct gattcttccc ggagacagcg ggaataccct gaagctcttg
     5761 gatatccaag ctttgatgaa cggagggggt cgacaatgaa aaccttatgg tggttcctcg
     5821 ggattttcct cgcctttttc atcctgttcg cgatggtgac ctatacggtg gatgaaacgc
     5881 aactggccat ccgtatccgt ttcggaaaga tcctggacgt ccacgatacg gccggtatcc
     5941 atttcaaagc cccttttatc gataggatag aaaagcgcga caagcgcctt atgctgtacg
     6001 atatcccgcc cgaaagcgtt ctcaccgccg acaaaaaacg gctggaagtg gatagcttta
     6061 tcctgtggag gatttccgac gcgaaagcct ttctggaaac gcttaacaat accgtcagcg
     6121 cgttaaacca aatcgacgat atcgtctatg cgaacatccg cgattctttc gcaaaattga
     6181 cgatcccgga catcatctcg gaagagcgtc gagccttctt gggatccatc acggtacaga
     6241 cctcggaaaa cctgaaaacc ttcggcgtgc aggtcgccga cgtcaacgtg aaacggaccg
     6301 atctgcccga gaccaatgcg aaagccgtat ttgaacggat gaaatccgaa aggtatcagg
     6361 cggcggccaa gattcgggcc gaaggggaga aggagtataa agagatcgta gcgaaagcgg
     6421 agaaagaatc atcggtgttg atcgccgagg cgaacaaacg cgcggaagag attcgagggg
     6481 aaggagacgc gtcggctcta gaggtttacg cctcagcctt ttcgaaagac ccggagttct
     6541 accgtttctg gcgctctttg agcgcctatg agaacgtttt ccaaaaagaa gcggtagtgt
     6601 tgctcgacga gcgggtcgat tttttacagt atctcttttc acataaagcc gagtagggaa
     6661 agatgggcgt cggtccgagc gcggattctt acttatcggt gcgtgcttca gcctttgatc
     6721 ggggtgaaac tgcggaggaa tcgacagaaa cgacgatcgt gatccaaaga tcgaggttta
     6781 tcggttgcgc ggctcgcgtt ctatcgaaag atgacgtgac ggacttcatc aaatcgagaa
     6841 aagcgaggca tccgcaagcc aatcatcacg tttgggcgta cgtcatcgac gttaaaggcg
     6901 gaagccagca tttttccgat gacggcgaac ccggagggac cgccggcgcg cccgtttttg
     6961 gagttttgtc caagcatcgg ttggaaagga cggccctcgt cgtgacccgg tatttcgggg
     7021 ggattaagtt gggaatcagc gggttgatcg aagcctatcg tcgtagcgcg gaagagacca
     7081 tccaggccgt tgggatcgcg cgttaccggc tctatcgtcg atacgctttc gcgttggcct
     7141 accccgactg gaaagggttc cagcgctttt tatccgctct cggggttgag gaaaacggga
     7201 tcgaagtccg atacgccgaa aaggtcgagg ttctggtaac ggttttagtc gaaagtgcgc
     7261 cgcgtctacg agaagagttg gaccgtttta tcgcgatgcg gaagatcata gggtatacgg
     7321 agagcgaccc ggctttcgtt ccggacctat ccgaaacgga taccctttag cggagcgttt
     7381 tgagtatgag cgtatgggaa gcgttggtgt tcagcatctc gttaatcacc tcgaacctgt
     7441 tgattctcaa cggaattgcg aagttcgacc tcaaggggaa gacccctttt accttcctcc
     7501 tgatggcctt cgccacatcg accgggtacg gtctgctccg ttatgttacg atcattctgt
     7561 atgacgactt ctatctggtc accaacatct tgtttttttt gaacaacctc tggcttttag
     7621 tcttcttcag aaacgcggtc gttcagtaca acgaccgtat cccgatcggc cgctattttg
     7681 tcctgtcgat cgtctgtttc ctcctcccga tatctatcgg gctatttttc aacccgataa
     7741 acaactttga aacgctgatg gttcagatgt cctatatgtt gggcatcatc ccgatggtcg
     7801 tctattcgtc gatgttagcc ggagtctccg ttggctccgc cttctggggg atcatcatcg
     7861 cgtgtttctt attctcggtg gatttcttcc tgcgtttcgg gtttttccat atcctacaaa
     7921 ccgaatttca agaagtgatt tcggagacct tcagagtcgt ctctatcttg ctgatcagca
     7981 tcttcggcca ctacggacaa ccgattccac ccgggaaaat acgggaggtc atcccgggta
     8041 cgcaacgcct gaacaatatt ttcttcgcgc tcatcgcgtt tttcccagtt atgatctttt
     8101 acaccgtttt ttggcaggtc aatctgaacg accgaaaaat gcagcaaaaa gagatcgagc
     8161 tttccaagga agtcttcatg atcagcagta ggatgctcga agaaaagatc gatacctatt
     8221 cgcggatcgt caacgaggtt aaaaattcgg cgaacctcgc gacggcgcaa tggaagctaa
     8281 agaccttcct attcaccgat ccggaaatcg acgtcgcaca gcttctgacc tcttatccga
     8341 ttatgtacaa agatgaggaa gtgatcttcg agaagggccg cgtcaccttc tacacccgaa
     8401 cggaacaagg gtggggcatc aagctatcgg tcaatatgga ggttatgtac gctcaatttc
     8461 ccgtcaacga atctcaaagg ttctacgcgt ttttaaacga tacgaccttt ttgtatccgc
     8521 ccggccaaat gatcgaagcg aacgccctct tctccagtat tggtaaaagg gatttcgtct
     8581 tcgtcaacac gatcaacctc tataaaaaac cattcagcac ctatctcata ttcaccacct
     8641 ccccgatcac ggtgaacatc tacacgcttg cctttatgct gggggcgata ttcatcctgg
     8701 ttttagtttg gtggttttac ggtttttacg tccatcgatg ggagaaaacc gcaaaagaga
     8761 agtatttttc gttgaatcaa gctaaagagg agtatggcga acagatcgga cgtttagaga
     8821 gctcgttgaa gctcatatcg ggagacttgc agatcacggc ggacaagctg gaccagttca
     8881 gccgcacggt ctatcaactc accgacagtt tctccggctt cgatttccag aaaggcccat
     8941 ccttcgaatt gaaacagctg tacgataagc tg
//
LOCUS       DAOPXN010000083         8909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7859_length_8909_cov_5.381974, whole genome shotgun sequence.
ACCESSION   DAOPXN010000083 DAOPXN010000000
VERSION     DAOPXN010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7859_length_8909_cov_5.381974"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3116)
                     /locus_tag="PKM49_05935"
     CDS             complement(<1..3116)
                     /locus_tag="PKM49_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82390.1"
                     /translation="MRKYVWVFVVCLAFCLLMGSCQSQPEITDIEQLARQSIGVQTGN
                     IADKMVLSRFPNAKIVYFVKPMDAVLSVINGKIAAFAADALSLEYIAGSNDEVSMLPG
                     YIVPDYYGFAVRLGDDALKAAIDATLAEIKRDGTYEAMRARWFPKSGEPEPMPDIPLT
                     GENGTLVFGTCSEQMPFSFVDNNRKPAGFDVEIATYVAKRLGMKLEIVDMEFGALITS
                     LEAGKVDMIGASISITEERKKRVLFSDSYYSAGLGALVKAPKAPAPERIKKLYTRPDD
                     LATARIGAMTGSIGETTIRERYPNADLRIFDDIMDAVAALKAHQIDAVITLYTTALNA
                     ARNNPDLDLLPGDLTQESAGVVASKQRPELLADVNRVLSKLKADGTLDDMVRRWILED
                     YREQEPVEIPMPPSGPTLKVGVAANRVPVEFVAGDGTITGLDPELACRIAAGLGMRVE
                     FHDMKFAALINALESGKVDLIIANMTRTEERARMVDFSEPYFSNPNRFLIRKEDDGSS
                     LGLAVPEDVNGKTVGVFTGSAQDAYISKTYPQAKVFRFNTLSKMENALKSGTVDALMY
                     SHEVFLEAARLDETLGLLGGPVFSIPIGMGFSQDNTPLCDQFNHFLRELRSGGTYEDI
                     VDRWMNRRVYEIPAIVNDGRNGSLAIGIVSDVSLPFAAQKDGRPIGFDIEIAERFAAY
                     VGKKPVFLTLTSENLAGALSTGEVDMIAASMFLSEEGDSQPLLSEPYYEMGASFLALK
                     KNLAAYEGARAPIERKSFFERLANSFYINIILENRYLLILSGLRTTLVISVLACLFGT
                     ALGAGICALRMSKNVILRWLAKVYISVIRGMPVLVLLMLIFYVIFASVNIDPVLVAVI
                     AFGMNFGAYVSEMFRASIEGIDKGQREAGWAGGFTKLQTFRYILLPQALKSVLPVYKG
                     EFISLVKITSIVGYIAVQDLTKASDIIRSRTFDAFFPLIMVAVLYFLLSGVLIQILSL
                     VEVQVDPKARRKAAASVSTNPIKRAWLRYKPFVYLILFAVVVLAALITPLLENARGVS
                     EGPIRSLA"
     gene            3941..5314
                     /locus_tag="PKM49_05940"
     CDS             3941..5314
                     /locus_tag="PKM49_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82391.1"
                     /translation="MRKALLVSTILLLLSLLFVSCATKGIDYGLVLDNSSPFSIREGG
                     TYGPNSVLELAWKGLEGREIIADVSVMLNNVKIKEFFGVTKLTFTVEEEGSYVAIITA
                     KGARGNTKAVNFSVNRLWAESLSSFNGSSDGLFYFDTCSSLLRSWVLNGHSPFIAFYG
                     LEQNVIANVVPETENVRTRLTLVDTDDDGVYDAWQEVATYAQQKIPLPVREHPTYGLL
                     VPHQLIYTTDRIEIWQWLILTGQYAGYLLNTAQPTLFASVGFELNWPYTRRLDTYEEE
                     SNYAYGQIIKLHALYDSDKVPMFSLDVALGNPARDIAFTAVITAENVARFGQIYNTRY
                     MQIPVFFSTNIAFRGITFDNFMPGLSDISSYRVIYDVINDEGEDCNILMIYRGFLEGA
                     DEPVATTPVFASIDFETLDDEPGFIALDYIDEYGADVMPVFKDNQNKNVDGFVIDYDT
                     WIPVNGI"
     gene            5333..6748
                     /locus_tag="PKM49_05945"
     CDS             5333..6748
                     /locus_tag="PKM49_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNY82392.1"
                     /translation="MKKAISLFTALLFLSLSLFAVSDAQPFIEKVNASIENMGLSWKA
                     GFKDAFLDIYGEGISTVEEMGRVLNQYIFIEKELEIGRAMGSRLTTTDDMLMSSLQII
                     EPLTDITVPTFYRLEPIRDQYLYGSCWAFGTVAAFESALAVQTLGKREIGVGNTDNTY
                     DFSERWVGFHNINWSLYQWNNWYYGFYLQDHDRLNGGNAYFAHYNDIRYGMIEEKNAP
                     YSDIFLSNLEGLPLPATAWTAPRTYSTRTAIAPTPRTGVFETRDEYVNNIKNLLYNYG
                     SLTVSYTVLDDFNGYERGIYTPTATEVGGGHCVTLVGWVAAEDLDKVVLGGKLNPGAL
                     TIVDDPISELKYYDPTVEATRTATEFWIIKNSWGYAWGDGGYFVMPIISEEDFDNGWF
                     PNWQIEYNHMYVPLFDRLELHEADSLDINGDGVVNATDYQFLVSLIGTTNPAGDIAYP
                     KDGKVTYEDVSTWVFLYNARY"
     gene            7275..8906
                     /locus_tag="PKM49_05950"
     CDS             7275..8906
                     /locus_tag="PKM49_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82393.1"
                     /translation="MKKPLLLLLLTLCVTLLFVSCAGIVRPAGVLRFVVTNWNSGPGV
                     VGATVEVYESGTANRVGHGVTGALGAVEIDVSTLPAKIDVKVTKQGFARSLVQGLKPE
                     TPGETPFGIIMKTAILNPDPATETDPVVRIGFFGIETGIGDGRAGAPIDINTQPITGP
                     FTVKVEVTAFHHITCIYAPHIGRVPGDSDVTFDQDHVENDTEAEFDITPIGHDGEVAL
                     YTAVYDYNGNRALKVDYLQVNATDPEEVAIYQPMTLSELADRYFDGEFALENIWTYTR
                     RRGKKLDVDPREARILGKAEPLHPSERIESHWGSDPLEGSRVAPESGNLWSHLFWTDW
                     RTAYQRHIDDPGKFPNPGDEPDGYNLYYSLDGVNYAKLGFVAETSVAEFCELILFFIG
                     IDFDVNPVMNSFALYKDPSIFLHPGVRMHYRLTSVYGTLESTPTYLGSVVPLDSFNIE
                     LENPVDDEVDVSLNPVLKWKPTKALESPEGDVTYTYAPFIYDWVQSDSGLIFPVTLAG
                     KLALFTSDSASSIELPFTGANPSDPIIGWTWYNPY"
BASE COUNT         2073 a         2420 c         2292 g         2124 t
ORIGIN      
        1 tcggccaagg agcgtatcgg gccctccgag acgccccgcg cgttctcgag cagcggagtg
       61 atcagggctg ccaagacgac gacggcaaag agaatcaggt atacaaaggg cttgtatcgt
      121 aaccaggcgc gtttgattgg gttcgtagac accgacgccg cggccttccg cctggccttc
      181 ggatcgacct gaacctccac caaagatagg atttggatca ggacgcccga caacaggaag
      241 tacagcaccg ccaccatgat caaggggaaa aacgcgtcga aggttcggct tcggatgata
      301 tcgctcgcct tcgtcaagtc ttgcaccgcg atgtacccca cgatcgaggt tatcttcacg
      361 agcgagatga actccccctt gtagaccggt aggacgcttt tcagggcttg gggtaagagg
      421 atgtaccgga aggtctggag cttcgtgaat ccccccgccc aacccgcctc tctttgtcct
      481 ttgtctatac cttcaatcga cgcgcggaac atctccgaga cgtacgcccc gaagttcatt
      541 ccgaaggcga tcacggccac cagaaccgga tcgatgttca ccgacgcgaa gatcacgtag
      601 aagatcagca tcagcagcac caacaccggc atccctcgga tcaccgagat gtatactttt
      661 gccaaccatc ggagtatcac gttcttcgac atcctgagcg cgcagattcc cgcccccagc
      721 gccgtcccaa agagacacgc cagcaccgat atgaccagcg tcgtccgcaa gccgctcagg
      781 atcagcaggt atctgttttc taagatgata ttgatgtaaa aactgttcgc caaccgttca
      841 aaaaagctct tccgctctat cggggctcgt gcgccttcat aggccgccag gttcttcttg
      901 agcgccagga aggaggcgcc catttcgtag tatggctccg ataaaagcgg ttgtgaatca
      961 ccttcttcgg acaaaaacat tgaggccgcg atcatatcga cctcaccggt tgagagcgcc
     1021 ccggccaggt tctcggaagt aagggttaaa aacaccggtt tcttcccgac atacgcggcg
     1081 aatctctcgg ctatctcgat atcgaatcca atcggccgac cgtctttctg cgcggcgaag
     1141 ggcaggctca cgtcgctgac gatgccgatc gcgagcgatc cgtttcttcc gtcgtttacg
     1201 atcgccggta tctcataaac ccttcggttc atccagcgat cgacgatgtc ctcataagtt
     1261 ccgccagacc ggagttctcg tagaaagtga ttgaactggt cgcaaagcgg ggtgttgtct
     1321 tgactgaaac ccattccgat cgggatcgaa aacaccgggc cgcctaacag gccgagcgtc
     1381 tcatccagcc gcgcggcctc cagaaagacc tcgtggctgt acatcaaggc gtcgactgtt
     1441 ccggacttta gggcgttttc cattttggat agggtgttaa accgaaagac cttcgcctgg
     1501 gggtaggttt tggaaatata ggcatcttgc gcggaacccg tgaatacccc gacggttttt
     1561 ccgttgacat cctccgggac ggccaatccc aaactgcttc cgtcatcttc cttcctgatc
     1621 aaaaaccggt ttggattaga aaaatagggt tcggaaaaat cgaccatccg cgccctctct
     1681 tcggttcggg tcatattcgc gatgatgaga tccacttttc cggattcgag ggcgttaatg
     1741 agcgcggcga atttcatatc gtggaattcg acccgcattc ccagtcctgc ggcgatccga
     1801 caggccagct ccgggtccaa cccggtgatc gtcccgtccc ccgcgacgaa ctcgacgggc
     1861 acgcggttcg ctgcgacacc caccttcaac gtcggtccgg atggcggcat cggaatctcc
     1921 accggctctt gctcccgata atcttccaga atccagcggc gcaccatgtc gtccagggtg
     1981 ccgtctgctt tgagtttgct aagaacgcgg ttcacgtccg ccaacaattc cgggcgctgt
     2041 ttcgaggcaa cgacacctgc cgactcttgc gttaagtccc cgggaagcag gtctaggtcg
     2101 gggttattcc tggccgcatt caaagccgtc gtatacaaag taatgaccgc gtcgatttgg
     2161 tgggccttca atgcggcaac cgcgtccatg atgtcgtcga agatgcggag gtccgcgttc
     2221 gggtagcgct cacggatagt agtttcgccg attgacccgg tcatagcgcc tatcctggcg
     2281 gttgccaaat cgtccggacg cgtgtagagt ttctttattc tctcgggggc tggcgccttc
     2341 ggcgccttca caagcgcgcc caacccagcg gaatagtagc tatcgctgaa cagtacgcgt
     2401 tttttacgct cttcggtgat cgagatgctg gcgccgatca tatccacttt gcccgcttcg
     2461 agagaggtga tgagcgcgcc gaactccata tccacgattt cgagcttcat cccgaggcgt
     2521 ttggccacat aggtggcgat ctcgacgtcg aacccggcgg gctttcgatt attgtcgaca
     2581 aaagagaacg gcatctgttc cgagcaggtg ccgaacacca gtgtgccgtt ttctcccgta
     2641 agagggatat cgggcatcgg ctcgggttct ccgctcttcg ggaaccagcg ggcgcgcatc
     2701 gcttcgtaag tcccgtcgcg cttgatttcg gcgagcgtgg cgtcgatggc cgccttcagc
     2761 gcatcatctc ccaggcggac agcgaaaccg tagtagtccg gaacgatgta tccgggaagc
     2821 atcgaaacct cgtcgttgga accggcgata tactccaaag acaaggcgtc cgcagcaaaa
     2881 gcggcgattt tcccgtttat gaccgacaag acggcatcca tcggttttac gaaatacacg
     2941 attttcgcgt tgggaaaccg cgacaaaacc atcttatccg cgatattccc tgtttgcacg
     3001 ccgatcgact gacgcgccaa ctgctcgata tccgtaattt ccggttggct ttggcagctt
     3061 cccataagca aacaaaacgc aagacagacc acgaacaccc aaacgtactt ccgcatatgt
     3121 cagactcctc tcgtcaacgc cagaaagccc cagcgctcgt catcagcgac ttcccatttc
     3181 cgcccggatc cccgtacccg cggatttccc cgcatctagg gattatacaa caatagacaa
     3241 ccgaaaaact tccgtgtggg gcaattttgg ggtgacataa ctaagaggtt catcgcgggg
     3301 taagtgattt ttacgactcc gcgatcctta ctttgtcttt tctataccgc gatgagtctt
     3361 ttcacaagcg tatcgcgccg tcgcaaaaag aagaagcgct tacgccgcga tgcccgtgtg
     3421 gggtaatcgc ggggtaagtg gtttttacga ccccgcgatc cgcactttgt cttttctaca
     3481 ccacgatgag tcttcacaaa agcgtatcgc gccttcgcga aaagaagaag cgcttacgcc
     3541 gcgatgtatg ttttctccgt gccctctgtg ttctccgtat gagcccctct tcttaaaacc
     3601 ataccgaacc aaagttcggc gttcctgtcc tcaaaaaaga atcctccgta ggctcaattg
     3661 caaatattct atcgcctcta ggaatttggg aatggatcga ataagaaagc ccgacgtata
     3721 tagaggtttg ataatcacgt gaaaaaccgc tcgttgaccc cgttagaaat cgttttacag
     3781 aaaactcgat ttgccttaac cgaccgactt ttttagaagt tattcgtgat atactatact
     3841 atgatagaag gggggattga tcgtccggat tttcaagcta cccttttttg cattgagtct
     3901 gatcgaaatc atcattttct aaaagaggag gtatttacag atgagaaaag cgttactcgt
     3961 atcaacgatt ttgctgctac tttcgttgtt gtttgtaagc tgcgcgacga aaggaatcga
     4021 ttatggactg gttctcgaca attctagccc gttttcgatt cgagaaggcg gaacctacgg
     4081 ccctaattct gttctggagc tggcttggaa agggttggag ggtcgcgaga ttatagccga
     4141 cgtctctgtg atgttgaaca atgtgaagat caaagagttt ttcggcgtga caaaactcac
     4201 atttacggtc gaagaagaag ggagttatgt cgcgatcatc actgctaagg gtgccagagg
     4261 aaatacgaag gccgtcaatt tttctgtcaa ccggttatgg gcagagtctt tatccagttt
     4321 caacggatcc agcgacggat tattctattt tgatacctgc agttcgcttc tacgttcttg
     4381 ggttctgaac gggcactcgc ctttcatcgc gttttacggc ttagagcaga acgttattgc
     4441 caacgtagtg cctgaaacgg aaaacgtcag aacacggtta accctcgtgg atacggacga
     4501 tgacggtgtt tatgacgcgt ggcaggaagt cgcgacttat gcccagcaga agattccctt
     4561 acccgtgagg gagcacccga cctacggcct tttggttccg catcagctca tttacacaac
     4621 ggatcggata gaaatctggc aatggctgat cctcacaggg caatatgctg gctacttgct
     4681 caacaccgca cagccaactt tattcgcttc ggtcggtttt gaactgaatt ggccttacac
     4741 gcgacggctg gatacctacg aagaggaatc gaactatgct tacggacaaa tcatcaagct
     4801 ccacgcctta tacgattcgg acaaagtccc gatgttctcg ctcgatgtcg ccctcgggaa
     4861 ccccgccagg gatatcgcct tcaccgccgt gattacggcc gaaaatgtcg cccgattcgg
     4921 ccagatttat aacacacggt atatgcagat accggttttc ttctccacta acattgcatt
     4981 tagaggaatc acattcgata acttcatgcc cggattatct gacatctctt cctatcgggt
     5041 aatctacgac gtaatcaacg acgaaggcga agattgcaac atcttgatga tctatcgcgg
     5101 gtttttggaa ggcgcggacg aaccggtcgc aaccactccg gttttcgcca gcatcgactt
     5161 cgaaactctg gatgacgaac ccggttttat cgcgcttgat tacatcgacg agtatggcgc
     5221 ggatgtgatg ccagtgttca aagacaacca aaacaagaac gttgacggat tcgtaatcga
     5281 ttacgatact tggatcccgg tcaatggaat ttaaggacga gaggtgatga cgatgaaaaa
     5341 agcgatctct ctattcactg cgctattgtt cctttctctc agcctttttg cggtttctga
     5401 cgcgcagccc ttcatagaaa aagtgaacgc atcgatcgaa aatatggggt tgtcctggaa
     5461 agccggcttc aaagacgcct ttttggatat ctatggagaa gggatttcta ccgtcgaaga
     5521 gatgggcaga gtgttgaatc agtacatttt tattgaaaaa gagctggaga tcggccgcgc
     5581 tatggggtcc cggttgacaa cgaccgatga tatgcttatg tcttctctcc aaatcatcga
     5641 gcccttgacc gacattacgg tcccgacctt ctaccggttg gaacccatcc gtgatcaata
     5701 tctctatggc agctgttggg ctttcggcac cgtggcggct tttgagagcg ctttggccgt
     5761 gcagactctg ggaaagcgcg agatcggcgt cgggaatacc gacaatacct acgatttttc
     5821 cgaacgttgg gtcggcttcc ataacatcaa ctggtcgcta tatcagtgga ataattggta
     5881 ttatgggttc tatttacagg accatgacag gcttaatggg ggaaacgcat atttcgccca
     5941 ctataacgac attcgctacg ggatgataga ggaaaagaac gctccttact ccgatatctt
     6001 cctgtccaac ctggaagggc tccccttacc agccaccgcg tggaccgcgc cgagaaccta
     6061 ttcgacccgt acagccatag cgccgactcc tcgcaccggc gtatttgaaa ctcgggacga
     6121 atatgtcaat aatatcaaaa accttctcta caattacggg tcattgacgg ttagctacac
     6181 agttttagat gatttcaatg ggtatgaacg cggtatctac acaccaacag cgacggaagt
     6241 gggcggcgga cattgtgtca cactcgtcgg atgggtggcc gcggaagacc tcgacaaagt
     6301 cgttctcgga ggcaaactca atccgggcgc gcttacgatc gtggacgacc ccatctccga
     6361 gttgaaatat tatgatccga cggtggaagc tacccgaaca gcgaccgaat tctggattat
     6421 aaaaaacagt tggggatatg cgtggggcga tggtggttac ttcgtcatgc cgatcatttc
     6481 ggaagaggat ttcgacaacg gatggttccc gaattggcaa atcgaataca accatatgta
     6541 cgtcccccta ttcgatcgcc ttgagttgca tgaagccgat agccttgata tcaatggaga
     6601 cggggtcgtg aatgccacgg attatcaatt cctagtgtcc ttgatcggaa ccacgaaccc
     6661 ggccggcgat atcgcctacc cgaaagacgg aaaagtcacc tacgaagacg tttcgacctg
     6721 ggtcttcctc tataacgcga ggtattaaac ctgtttttga ctaaaagccc ccgtacgggg
     6781 gcttttttta tacgcaagac gccgtgccat ctcgctcgtt ggatttttaa cgcttctgag
     6841 agcgccgcga ttactacgca gttcatcgcg gggtaagtga tttttacgac cccgcgatcc
     6901 gctctttgcc tttctaaaag cgtatcgcgc cgtcgcgaaa agaaggggcg cttacgccgc
     6961 gatatatatt ttctccgttc cctctgtgtt ctcagcgcaa aacccttttt tctttaaaaa
     7021 agctgccgaa ctaaagttcg gcgttcccag gcgtacccag aagttcggtg ttcccaggcg
     7081 cccttcgctt cgacacggcg ttcccaggcg ggtcgtcact gcgtcaccgg caaggggaag
     7141 gcatgtttca agggagcgct gtgttataat cccgcggtcg cgcgagcggt ttggggttat
     7201 gtcaggccga ttccacgtga tcggttctca tcgataccgc atccagatgt ggatttttat
     7261 atggaggtta ggccatgaaa aaaccgctcc ttttgcttct tttaactctt tgcgttaccc
     7321 tcttatttgt ttcgtgcgcc ggtattgttc gtcctgccgg ggttttgcgt tttgtcgtga
     7381 cgaactggaa ctcgggcccc ggggtcgtag gcgccaccgt agaggtctat gaaagcggca
     7441 ccgcgaatcg ggtcggacac ggtgtgacgg gcgccctcgg agcggtcgaa atcgatgtca
     7501 gcacgctacc tgcaaaaatc gatgtcaaag tcaccaaaca aggtttcgcc aggagcttgg
     7561 tgcaggggct gaaaccggaa acccccggcg aaaccccatt cgggatcatt atgaaaacgg
     7621 cgatcctgaa ccctgaccca gccacggaaa cggacccggt cgtccggatc ggattcttcg
     7681 gaatagaaac gggcatcggc gacggacgcg ccggagcccc gattgacatc aatacccagc
     7741 cgatcaccgg tccttttaca gtcaaagtcg aggtcaccgc tttccatcac attacgtgta
     7801 tctacgcgcc ccacatcgga cgggtccccg gcgactcaga tgttactttc gatcaggacc
     7861 atgtcgagaa cgacacggaa gccgaattcg acatcactcc catcgggcac gacggcgagg
     7921 tcgcgctgta caccgccgtt tatgactaca acggaaaccg cgcgctgaaa gtggactacc
     7981 tccaggtgaa tgctacggat cccgaggaag tggcgatcta tcagccgatg actttatctg
     8041 aattggctga tcggtatttt gatggagagt tcgcattaga gaacatatgg acctacaccc
     8101 gtaggcgcgg aaaaaagcta gatgtggatc cgagagaggc gcgaatactt ggaaaggcag
     8161 aacctctcca tccttcagaa cgcatagaaa gccattgggg aagtgacccg ctcgagggaa
     8221 gccgcgtcgc gcccgaaagc gggaacctgt ggtcgcacct tttttggacc gattggcgca
     8281 ccgcttatca gcgccacata gacgaccccg gaaaattccc aaaccccggt gatgagccgg
     8341 acggctataa cctgtactat tccctcgacg gcgtcaacta cgcaaaactc ggtttcgtcg
     8401 ccgaaacttc ggtggcagaa ttttgtgaac ttattttatt ctttataggc attgattttg
     8461 atgtcaaccc cgtaatgaat tccttcgccc tctacaaaga cccctcgatt ttcctgcacc
     8521 ccggtgtgag gatgcattat cggttgacct ccgtatacgg cacgctggag tccacaccga
     8581 cctatttggg ttcggtggtg ccgctggatt ccttcaatat cgagttggag aacccggttg
     8641 acgatgaggt ggatgtgtcc ctaaacccgg ttctcaaatg gaaaccgacg aaggctctgg
     8701 aatcgcccga aggcgatgtg acctacacct acgccccatt catctacgat tgggtgcagt
     8761 cagatagcgg gctcatcttt cccgtcacgc ttgctgggaa attggcccta tttacctccg
     8821 actcggcgag cagcatcgaa ttgcccttta ccggcgcgaa tccgagtgat cccattattg
     8881 ggtggacttg gtataacccg tactaggat
//
LOCUS       DAOPXN010000084         8885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_7891_length_8885_cov_5.499207, whole genome shotgun sequence.
ACCESSION   DAOPXN010000084 DAOPXN010000000
VERSION     DAOPXN010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8885
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7891_length_8885_cov_5.499207"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1172..3307
                     /locus_tag="PKM49_05955"
     CDS             1172..3307
                     /locus_tag="PKM49_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82394.1"
                     /translation="MFRKIKLMWRILLLIGLPIVITFVLVIVLATTSFVTREKENAAE
                     LVDYLASAYAQEIKANLDVAMDAVRTIAQIFENFENMALNERRNNFNFILKSALEKNK
                     DFLGVWTCWEPNALDGMDSQFANKDWYDATGRFVPYWSMSGGRADLVPLVGYDEPGPG
                     DCHLIPKQTGQEMIMDPYLYDVGGTKMLMTSVVVPIKRKGVVVATAGIDIDLLTIQKT
                     VEKIKPYETGVSAVFTNNGIVAAHFDSSRLGKDGKKSEIDMAGRNINQMFDAIKDGKN
                     FSFTTYSEGMKSDIAIYLEPFFVGNSKSSWTLAVGIPVNKVLDQANRLQLFLLIIGIV
                     SVALITAIIFFVSRSITRPITALGEVIEKVKNKDLSYNQNHRAVKYLSRQDEIGHIVN
                     ALAALEKEYTDTIALFQKSISELEGSAHRLASISQEQLTSSEALTAQAQKVDENVQNT
                     SASIEEVSSGVEEVAASAQSVSKTARELADQNEETSKKAKEGGSLISQVTKRIETATK
                     QTIDTAGLVQKLAEGAKNVEDILSTISSIAEQTNLLALNAAIEAARAGEAGRGFAVVA
                     DEIRKLAEESKKATTNIARILKDISNGAKGANDATGQTVEIVKEVNVSAQEVETQFSE
                     ILKMVEKTTSLVVALTATSEEQGAAAEEMASAMDNSAKASAEISNQTHRITQSAKQLT
                     EGAQQVETAAEQLNNLSEALEAEIQKFKL"
     gene            4541..5278
                     /locus_tag="PKM49_05960"
     CDS             4541..5278
                     /locus_tag="PKM49_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNY82395.1"
                     /translation="MKRATLFLTLVLVFSLFAFGGIIDTIKARGYLVMGTNATFPPFE
                     FVDAKNEVVGFDIDLARAVAKKLGVTLRVIDFAFDGLIPALQADKFDIIFAGMTITEK
                     RKEVVLFSDPYFDAGQVIVVRKGYKHFDDVDELTNEKVAVQTGTTGDLMATEVPGIIL
                     TRFPHFTEALMELSLRRVNAVVIDSSTGEAYIKFNPNLEVGSPILSAESYGAAIKKGN
                     EELLNFVNQVLKELKTSPYDALLDAWF"
     gene            5451..6092
                     /gene="ehuC"
                     /locus_tag="PKM49_05965"
     CDS             5451..6092
                     /gene="ehuC"
                     /locus_tag="PKM49_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="HNY82396.1"
                     /translation="MSDIILSMPFLLQGAVITLQVTVLSAVFGTIIGTIFGMGRLSKA
                     PFLRIPSSIYIEFVRGTPLLVQIYIIYFGLPSIGIDFPPFTAAVVALSINAGAYTAEI
                     VRAGIQSISRGQTEAARSLGMTVWQTMRYIILPQAFRNILPALGNEFIALTKESSLVS
                     VIGIAELTRQGQYIISRTFKSFEIYGGVAIVYFAITFTLAQLVNWVEKKVTIK"
     gene            6098..6820
                     /locus_tag="PKM49_05970"
     CDS             6098..6820
                     /locus_tag="PKM49_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HNY82397.1"
                     /translation="MISIRNLHKRFGHLEVLKGIDLELQKGEVMVVIGPSGGGKSTML
                     RCINHLEKYEKGEIYFKDVLLHEKRVNMNEYRMKMGMVFQQFNLFPHRTVIDNLMLSP
                     LKVKKMDPAEVKEKALNLLKRIGLSEKASAYPINLSGGQQQRVAISRALMMDPELMLF
                     DEPTSALDPELVGEVLEVMKQLAREGMTMIVVTHEMGFAREVGHRMILIDDGLIVEEA
                     TPTDFFQNPKSERTKEFLRKVL"
     gene            complement(6834..7736)
                     /locus_tag="PKM49_05975"
     CDS             complement(6834..7736)
                     /locus_tag="PKM49_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNY82398.1"
                     /translation="MLKEKLSGWISLIIVYIVWGSTYLAIRIAVQPGAGFEPFTLVTM
                     RLLVSAGFLFLLAYVTRQKMKIPRGERLAVLLPGVFQWLAGNGFITWAEKTVNSGYAA
                     LMVATTIIWVTIFEAIIEKKRLSAKTFLILSIGFVGLAFLNIPALGSAEGVDGFGVFV
                     LLFASASWGVGTLIQKYRRTSLTPTVSSAYQQLIGGLAVLVMRFILNEPLPTPTPAAW
                     WAWGYLVVFGGVLAYTAFIIAVRNLPTRIVMTYSYVNPLVALALGWIVLKEEVTVWTL
                     IGAGLIVLSVFGIYKTKSGSAPVD"
     gene            complement(7739..>8885)
                     /locus_tag="PKM49_05980"
     CDS             complement(7739..>8885)
                     /locus_tag="PKM49_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNY82399.1"
                     /translation="LENYEQAARLKAELEETRNRYQARYDEWKKTQETKPKVVNEFVI
                     AQLVEKWTGIPTTRMLHSEREKALNLEAMIHQKYVNQVDAVNALAQSIRRSKAGLKDP
                     KRPTGSFLFLGPSGVGKTELAKVLAEILCGSSEALVRIDMSEYSEKIAVSRLIGASPG
                     YVGYEEGGQLTEAVRRRPFSVVLMDEIEKAHPEVIHILLQLLEDGQLTDGQGHTVDFK
                     NTVIILTSNIGTEQLSKRQHRVGFAADSEELEKDKLVMDELKKYFKPELINRLDNIII
                     FRNLKPEDVQKIVDLMLERLNERLVDKHIKVDMTERAKDFLAKKGFDESFGARPLRRV
                     FEKHVEVPLSDMLLEGTIEENNDISVDLDEEAERLLFQIHQPDTRRV"
BASE COUNT         2240 a         2258 c         2330 g         2057 t
ORIGIN      
        1 aaaaacgata accctcgaat aagcccatct gtgaggagca caggaaacgc tgaattttag
       61 ttcggtagtg tttcctagga gcggcaactt tagtgcggta gggcttgctg gggacgcaga
      121 acttcagtcc ggtatgtgtt ttacctggga cgccagcttt taatttgaca tgtcttgaaa
      181 aacaaggctc tcacggaggg cacagagggc atggagagcc agggcgcgcc aaaaaaccaa
      241 agcctgacgg actcaagtcc cgccgctctg gcggcgctca gaaggcgttc ccagtttgcc
      301 acagttccac gtttgtttgg cggtcttttt tgttccttcc tccgaactca cttctcctca
      361 attcgctcct tttattgata cccacgccta aagagagcgt agataccccc cactccgtgg
      421 cggttgccct tctcgtcgaa aggtcgcgta ggggacaccc tatcggaaac caagggggtt
      481 atccgtccga gcgcatagcg tcaacccaaa cgcaataatc caacactctc gaaatgagaa
      541 agacaggtat attctataat aaaagtagct attcagaaaa taatatttac cgatcatagc
      601 gttaagatat taaccgtcat aacccaaaac cgaagtggcg agcgtcacct cacgcgcaat
      661 acatccggtg tctgggtcgt tcacgcattg tacttacccc tgtttctgcg ggaagttgcc
      721 acctgatttg gttaattttc cgtgtatttt taaaaaagaa atccgcacaa gtggtacaat
      781 ctccgtggaa attggaagac gacctccttc aacctcgtag tactatttca aggaagccgc
      841 cacacctagt cagtagtttt acctaattcg cttcacacga ctaggaagag aagggggaat
      901 caacggaatc gcgcgagcga tcgaacgact gggtacgacg ctcgccgatt gtttgaagaa
      961 cagaaacctc tcgagattta gctacggtag gtgttgaatc cagaaacgct ctcccagcca
     1021 acgagacggc ttggagtaag tagaaaggga tagacaggcg aagctatgag gagcgatacg
     1081 ttgcgggtgt atcgcgaccg gcaagctttt atcggaaaaa caataaaccg ctcctaaaag
     1141 acggagcgac aaaatgaaag aaggcgagat tatgttcaga aaaatcaagt tgatgtggag
     1201 gattctgttg ctcattggcc tacccatcgt gatcaccttt gtgttggtga tcgttctcgc
     1261 caccacctcc ttcgtaactc gtgagaagga aaatgctgcc gaattagtcg attacctggc
     1321 gagtgcttat gctcaagaaa taaaggcgaa tttggatgtg gcgatggacg cggtgaggac
     1381 gatcgcgcaa atattcgaga acttcgagaa catggcgtta aatgaaagga ggaataattt
     1441 caatttcatc ctgaagagcg ccttagaaaa aaacaaagat tttttagggg tgtggacctg
     1501 ctgggaaccc aacgcgctag acgggatgga ctcgcaattt gcgaacaaag actggtatga
     1561 cgccaccgga agattcgttc cctattggag catgtccggc gggagagcgg atttggtgcc
     1621 tttggtcgga tatgatgaac caggcccagg agattgccat ctgataccga aacagaccgg
     1681 gcaagagatg attatggatc cgtacctgta cgacgtcgga ggaacaaaga tgttgatgac
     1741 cagcgtagtc gtaccgataa agagaaaagg ggtcgtggtt gccaccgctg gtatcgatat
     1801 cgaccttctc accattcaaa aaaccgtaga aaagataaaa ccctacgaaa ccggtgtctc
     1861 agctgttttt acgaataacg ggatcgtcgc agcacatttt gactcttcgc gattggggaa
     1921 agatggcaaa aaaagcgaaa tagatatggc tggtagaaat attaaccaaa tgtttgacgc
     1981 aattaaggac ggcaagaact tttcgtttac gacatactct gaaggaatga aaagcgacat
     2041 agcgatttat ctggaaccct ttttcgtagg gaattctaaa tcgtcttgga ctttagcagt
     2101 gggaatcccc gtgaataagg tcttagacca agcgaatagg ttgcaactct tcctgttaat
     2161 catcggcatc gtgtcggtcg ccctgattac ggccatcatc ttcttcgtgt ccagaagcat
     2221 tacccggcct atcacggccc tcggcgaagt catcgaaaaa gttaagaata aagacttgtc
     2281 ttataatcag aatcatcgcg ctgtcaaata cctctcgcgt caagatgaga tcggccacat
     2341 tgtgaacgct ttagcagctt tggagaagga gtacaccgac acgatagccc ttttccaaaa
     2401 gtctatctcc gagttagaag ggtcggcgca tcgtttggct tccatttccc aagagcagtt
     2461 gacctcctcg gaagcgctga ccgctcaagc gcaaaaggtg gacgaaaacg tccagaatac
     2521 gtctgcgtcg atagaagagg tgtcttccgg cgtcgaagaa gtggccgcca gcgcgcaaag
     2581 cgtatccaaa acggctcgcg aactggcgga ccaaaacgag gaaacctcca aaaaagctaa
     2641 agagggtggc tcgctcatat ctcaagtcac caagaggatc gaaacggcca ccaaacaaac
     2701 gatagatacc gcaggcttgg tgcaaaaact cgccgaaggc gcgaagaacg tagaagacat
     2761 cctgagtacc atctcttcga tcgcggaaca aacgaacttg cttgcgctaa acgcggccat
     2821 agaagcggct cgagcgggag aagcgggaag ggggttcgcc gttgtcgcgg atgaaatcag
     2881 gaaattggct gaggaaagca aaaaggcgac taccaacatc gcgagaatac tgaaagatat
     2941 ctctaacggg gccaaagggg cgaatgacgc caccggtcaa accgtggaaa tcgtgaaaga
     3001 agtgaacgtg agcgcacagg aagtggaaac gcaattcagc gaaatcttga agatggtaga
     3061 gaagaccacc tctctcgtcg tcgccttaac cgccacttcg gaagaacaag gcgccgcagc
     3121 ggaagagatg gcctccgcga tggataactc cgcgaaggcg tcagccgaaa tctcgaatca
     3181 aacgcatcgc atcacgcaat cggccaagca gctcaccgaa ggcgcccagc aggttgaaac
     3241 tgcggcagaa caacttaaca acctttccga agcgttagag gcggaaatac aaaaattcaa
     3301 gctataggtc tttaggcctc aaaaggtaac gagagagcgc cgccacgggc ggcgctcttt
     3361 tattggagcg tcgcatttcg aaaggtttcc tgggaacgcc gaacttcagt gtcgtagggc
     3421 cctgggaacg ccaactttta tgttggcatg ccttgaaaaa cagggtttcg cacagagagc
     3481 acagagaaca cggagcggac aagcatcgcg gcgtaagcgc cttttctttt cgcgacggcg
     3541 cgatacgttt ttgtacgagg gcatcgcggc gtaagcgttc cttctttttg cgacggcgcg
     3601 ataccccggt tttcgttcgt tccaaagaaa aaaaacaatg ggttcgcacg gagggcacag
     3661 agaggacaag catcgcggca taagcgcccc ttctctttgt ggggccgact ttcagtcggc
     3721 ctttctctac gaactcttgg gaacgccaac ttttatgttg gcaggtcttg aaaaacaatg
     3781 ttctcacgga gtacacggag ggaatacgaa aaacgagata ggcgaaaagt agatcggttg
     3841 ctcctacgaa aagcgcttct agcataaata tcacagacag gccgaactaa agttcggcgt
     3901 tcccagtggg gcgctcctac gcatttggtg ctctttgtgg cgttcttaat tgttcctatc
     3961 ccattgccca tttttcctct gtgttctctg tgcaaaacct cattccactt accccgcgat
     4021 aaccccagac gggcatcgcg gcgtaagcgc ttcttctttc cgcgacggcg cgatactctt
     4081 ttaggaaaaa caaagggcgg atcgcggggc ttcatcagta tcgcgagact tcgtgggctt
     4141 ccgaagtcag cccgctggtt accccgcgat aaccccagac gggcatcgcg gcgtaagcgc
     4201 ttcttctttt tgcgacggcg cgatacgctt taaagaaaga cgaagggcga atcgcggggt
     4261 cgtaaaaacc acttaccccg cgatgaaccc ctaatacgtt cccagggtgt accctgaggg
     4321 tgtgttttaa aattccgctt gctgaaaaat cggaatgttt gtgatatact ctcttcatag
     4381 acttatcgaa aaacttccaa gagattaggt aacacatcgt accgttgtcg tcaagtgcct
     4441 gagagttttg tgatagatat gcgatggtta tatatccggt agagccgact ttgcctaacc
     4501 atcgttttta agcaaaccaa aatcatttgg aggtgtcaca atgaaaagag caacgctgtt
     4561 tctcacgctc gtcttagttt tctctctctt cgcgttcgga ggaattatcg acaccattaa
     4621 agctcgtggt tatctcgtga tggggacgaa cgccaccttc ccgcctttcg aattcgtcga
     4681 tgcgaaaaat gaggtcgtcg ggttcgatat cgatctggct agggcggtgg ctaagaagtt
     4741 aggggtgacc ttacgggtca tcgacttcgc gtttgacggg ttgatccccg ctttgcaagc
     4801 cgataagttc gacattatct tcgcgggtat gacgatcacc gaaaagcgga aagaagtcgt
     4861 actcttcagc gacccgtact tcgacgccgg tcaggtcatt gtagtacgaa agggctataa
     4921 acacttcgac gatgtcgatg aattaacgaa tgagaaggtg gccgtacaaa ccgggaccac
     4981 cggagacctg atggcaaccg aagtgcccgg gatcatcctg acgagattcc cgcacttcac
     5041 cgaagccctc atggagctca gcctcagacg tgtgaacgcc gtcgtgatcg actcctcaac
     5101 cggagaagcc tatatcaaat tcaatccaaa cctcgaagta ggcagcccca tcctttcggc
     5161 cgaaagctac ggcgccgcga tcaaaaaggg caatgaggag ctccttaatt ttgtgaacca
     5221 ggtgttgaag gagctcaaaa ccagcccgta cgacgcgttg ttggacgctt ggttctaaac
     5281 tgaccctacc gcagatcgtt tttttatatc catacgcatg ccgacggaat ccctccgtag
     5341 gcatgccttt attatgtgtt atgcgggcgc gtcgcttgtc ttcccactgc tttccacgcc
     5401 cgtataccca gtcgcatcaa cgcgctctta tctcgtttca ggaggcaact atgtcagata
     5461 tcatcttatc tatgcctttt ctactgcagg gggcagtgat caccttacag gtgaccgttc
     5521 tatccgcagt tttcgggacc atcatcggga cgatcttcgg aatggggagg ctttcaaagg
     5581 ctcccttctt gaggatacca agctccatct atatcgagtt cgttcgaggt acgcccttat
     5641 tggttcaaat ctacatcatc tacttcgggt taccttcgat cggaatcgat tttcccccgt
     5701 ttacggccgc cgtggtcgcg ctctctatca atgccggcgc gtataccgcc gagatcgttc
     5761 gggccgggat ccaatcgatc tcgaggggcc aaaccgaagc cgctcgttcg ttggggatga
     5821 cggtgtggca gacgatgcgc tatatcatcc tgccgcaagc ctttcggaat attcttccgg
     5881 ctttgggaaa cgagttcatc gccctgacga aagaaagctc gctggtgtcc gtcatcggga
     5941 ttgccgaact cacacggcaa ggccagtaca tcatcagcag aacctttaag tccttcgaaa
     6001 tctatggggg ggtggcgatc gtgtactttg ccattacctt caccttggcg cagcttgtga
     6061 attgggtgga aaagaaggtg accattaaat gagtacgatc atctctatac gaaatctgca
     6121 caaacggttt gggcatctcg aggttctcaa ggggatcgat ctggagcttc agaaaggcga
     6181 ggtgatggtg gtcatcggcc ccagcggggg aggcaagagc acaatgctcc gttgtatcaa
     6241 ccacctcgaa aagtacgaaa aaggggagat ctatttcaag gatgtcctct tacacgaaaa
     6301 acgggtcaac atgaacgagt accgtatgaa aatggggatg gtctttcaac agttcaacct
     6361 ctttccacat cgaacggtca tcgacaacct gatgttgtcc ccgttaaaag tgaaaaagat
     6421 ggatccggcg gaggtcaaag agaaggcgct taacctcttg aagcgaatcg gtctgagcga
     6481 gaaagcgagc gcctatccca tcaatctctc gggcggtcag cagcaaaggg tcgcgatctc
     6541 gcgcgccttg atgatggatc cggagctgat gctcttcgat gagcccactt ccgctttgga
     6601 tccggagttg gtcggggaag ttctggaagt gatgaaacaa ctggcccgcg agggtatgac
     6661 gatgatcgtc gtcacgcacg agatggggtt cgcccgtgaa gttggccatc ggatgattct
     6721 catcgacgac ggtttgatcg ttgaagaagc aacgcccacc gacttttttc aaaacccgaa
     6781 gagcgaacgt accaaagagt ttttgcggaa ggttctatag aaagtcgagt cgatcagtct
     6841 acaggggcgc tcccgctttt tgttttgtat atcccgaaaa ccgagagcac gatgagcccc
     6901 gcgccgataa gtgtccatac ggtgacctcc tctttcagta cgatccaacc cagcgcgagg
     6961 gcaaccagcg ggttgacgta gctgtaggtc atcacgatgc gtgtcgggag gttcctgacc
     7021 gcgatgatga acgcggtgta ggccaatacg cccccaaaga caaccaaata tccccacgcc
     7081 caccacgccg ctggcgtggg ggtgggaagg ggctcgttta ggatgaatcg catcacgaga
     7141 acggcgagcc cgcctatcag ttgctgatag gctgagctga ccgtgggcgt taggctggtc
     7201 cgccgatact tctgaataag ggtgccgacc ccccaggagg cggacgcgaa cagaaggacg
     7261 aagaccccaa acccatcaac cccttcggcg ctgcctaatg cggggatgtt gagaaaggcc
     7321 aatccgacaa atccgatcga aaggatcaag aaagtcttcg ccgacaatcg ctttttttcg
     7381 ataatggctt cgaagatcgt cacccagata atggtggtgg cgaccatcag ggcggcataa
     7441 ccggagttaa cggttttttc tgcccaagta atgaatccgt tacccgccaa ccattgaaaa
     7501 acccccggca ggagcacggc caagcgctca ccccggggga ttttcatctt ctgccgggtg
     7561 acataagcca agagaaagag gaagccggcg gagacgagga gcctcatcgt gaccagcgtg
     7621 aaaggctcaa atccggcacc cggttgcacc gcgatcctga tggccagata ggtgcttccc
     7681 caaacgatgt aaacgatgat gagactaatc catccgctca gcttctcttt caacatatct
     7741 aaacccgccg ggtgtcaggc tgatggattt ggaagaggag ccgttccgct tcctcgtcca
     7801 gatcgacgga gatatcgttg ttctcctcta tcgtgccttc caacagcata tcggagagcg
     7861 ggacttccac gtgtttctcg aagacccttc gcagggggcg agcgccgaag ctctcatcga
     7921 accctttttt cgctaagaaa tccttggccc gttcggtcat atccactttg atgtgtttgt
     7981 ccactagccg ttcgtttagt ctttcgagca tcaggtcgac gatcttttga acatcctccg
     8041 gtttgaggtt ccggaagatg atgatattgt ccaagcggtt gatgagctcg ggtttgaagt
     8101 acttcttcaa ttcgtccatc accagtttgt ctttttcgag ctcctcgcta tccgcggcga
     8161 agcccacccg gtgttgtcgt ttcgagagtt gttcggtccc gatgttgctg gttaggatga
     8221 tcaccgtgtt cttgaagtca actgtgtgcc cttgcccatc agttaattga ccgtcttcca
     8281 gcagctgcag caggatatgg atcacttccg gatgggcttt ttcgatctcg tccatcaaga
     8341 cgacagaaaa ggggcggcga cggacggctt cggtcaactg tccgccctct tcgtaaccga
     8401 catatccagg tgaggcgccg atcaggcggg agacggcgat tttttcactg tactcggaca
     8461 tatctatacg cactagggct tccgagctcc cacaaaggat ttcggccaac accttcgcga
     8521 gttcggtctt ccccacacca ctcggcccca ggaacaaaaa agatccggtg ggccttttgg
     8581 ggtctttcag gccggctttc gagcggcgaa tcgactgcgc caacgcgttc acggcgtcga
     8641 cctgatttac gtacttctga tggatcattg cctcgagatt gagcgccttt tctctttcag
     8701 aatggagcat ccgagtggtg gggataccgg tccatttctc gacgagctgc gcgatcacga
     8761 attcgttgac caccttgggc ttcgtttcct gggtcttctt ccattcatcg tagcgcgctt
     8821 gatagcggtt tcgcgtttcc tccagctcgg ctttcagacg cgccgcttgt tcgtagttct
     8881 ctagg
//
LOCUS       DAOPXN010000085         8780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8015_length_8780_cov_5.061089, whole genome shotgun sequence.
ACCESSION   DAOPXN010000085 DAOPXN010000000
VERSION     DAOPXN010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8780
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8015_length_8780_cov_5.061089"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            618..1274
                     /locus_tag="PKM49_05985"
     CDS             618..1274
                     /locus_tag="PKM49_05985"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNY82400.1"
                     /translation="MAFSFEKNLEYMLRITLAMLGGLAIGINREKLQRPAGLRTHALI
                     AVGSAFFALLSGEYFGGILGGDPGRIAAQIVTGIGFMGAGTIMRRGFTVTGLTTAATL
                     WVSASIGVGFGVGEFYLGGFMTLAVLLTLTVVKRLGLALSKNRTRFVIEMVYGKNGFQ
                     KVVDWLFENRITAGSLYYETDGERVTISISVDPDTVERLKQKWSELLNFKEIRSLEIK
                     "
     gene            1271..2047
                     /gene="dapF"
                     /locus_tag="PKM49_05990"
     CDS             1271..2047
                     /gene="dapF"
                     /locus_tag="PKM49_05990"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNY82401.1"
                     /translation="MICVRYAACGNTFTIVFGEVIEDKEKPKVVRSIVAEMDGVIFVE
                     KTESSFFMDYFNRDGSRGEMCGNGARAFIKALYDRGECALGQLLVFDTYSGILYGRML
                     AADRAEVLMPQPEFLSMFHHEGWKGAFYSVGVPHLAFFMRTVSGVDVEGYGYYFSHLR
                     DNSSGVSPFPVSTNVNFFSEEDEGLFVRTYERGVWRETASCGTGATACAAFYRKMTGQ
                     QSSAIRVTTKGGVLTVRFDRPHPQKEGEATYLEGTIEHYP"
     gene            2059..2898
                     /locus_tag="PKM49_05995"
     CDS             2059..2898
                     /locus_tag="PKM49_05995"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNY82402.1"
                     /translation="MKLFISLDMEGIAGTFNWQQETTQDRALVRKWMAQQIEWVIEGI
                     RQSPKNAMVTEITLADSHSNGDNLAYDITGLDERLSLISGNPRPNYMMPTLDGSYDTV
                     FLIGYHAGAGTPYGNMDHTYSNSTIHSLWINGKAMNETFINSAYASCFSIPVSLIVGD
                     KALKDQVMVEGGMPWVEFVVTKEALFKFAAKQRPLECVREETSKAVQKALAKDFKKLP
                     LYGFQKPYELKIEFQTSNQADFASMIPLVERIDGRTLRFVSEDFKQIFEACIYLPALA
                     RQG"
     gene            2901..3761
                     /locus_tag="PKM49_06000"
     CDS             2901..3761
                     /locus_tag="PKM49_06000"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY82403.1"
                     /translation="MRIGVVSPSSLPKSERLFKGLSLLRDQESQLIIGKSCFHYLSPL
                     ERAEELEKMEDICDLLLCSRGGCGSFQLAPFLTRGFKIPICGYSDITFLLLWQYAHGE
                     RAFHGPMLCDFQRPTDIQPSFLPFLRNDITLPYRYPGMKRTWPLRLGQAVGRLLAVNL
                     SLLIACLPFFGDKLLDGSVLFLEDVSESVDSIERYLWALRHYPTFARVKGVVFSALTE
                     TRRASNDYRLRDVLTRWAQEAGIPCFIGFPAYHGKYIKHCLPIGGTVEIDSETGTVTL
                     LSLGGALWNS"
     gene            3749..4426
                     /locus_tag="PKM49_06005"
     CDS             3749..4426
                     /locus_tag="PKM49_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82404.1"
                     /translation="MEFLALPLPEMIVSLEAQYRWEDALRCLRIWLQKDLDAALRRRL
                     EYEIFRIARLQKSYVHDEKTAYSMLEEAIVDFSKEEFDRLVRERALDCIRKDDGLYFE
                     KRFLPNLFFAFPDYVSRRRTVEESREKAKKHLESRLDALLRGEKPMRYRVIGRIQKRL
                     KPSALEALEGRHGGLECWLPFPLEDLQQTKPALIAASAPEYTLPHRFSETRAIHMDRA
                     RFRKAVR"
     gene            complement(4355..5032)
                     /locus_tag="PKM49_06010"
     CDS             complement(4355..5032)
                     /locus_tag="PKM49_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82405.1"
                     /translation="MEFLALPLPEMIVSLEAQYRWEDALRCLRIWLQKDLDAALRRRL
                     EYEIFRIARLQKSYVHDEKTAYSMLEEAIVDFSKEEFDRLVRERALDCIRKDDGLYFE
                     KRFLPNLFFAFPDYVSRRRTVEESREKAKKHLESRLDALLRGEKPMRYRVIGRIQKRL
                     KPSALEALEGRHGGLECWLPFPLEDLQQTKPALIAASAPEYTLPHRFSETRAIHMDRA
                     RFRKAVR"
     gene            complement(5020..5880)
                     /locus_tag="PKM49_06015"
     CDS             complement(5020..5880)
                     /locus_tag="PKM49_06015"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY82406.1"
                     /translation="MRIGVVSPSSLPKSERLFKGLSLLRDQESQLIIGKSCFHYLSPL
                     ERAEELEKMEDICDLLLCSRGGCGSFQLAPFLTRGFKIPICGYSDITFLLLWQYAHGE
                     RAFHGPMLCDFQRPTDIQPSFLPFLRNDITLPYRYPGMKRTWPLRLGQAVGRLLAVNL
                     SLLIACLPFFGDKLLDGSVLFLEDVSESVDSIERYLWALRHYPTFARVKGVVFSALTE
                     TRRASNDYRLRDVLTRWAQEAGIPCFIGFPAYHGKYIKHCLPIGGTVEIDSETGTVTL
                     LSLGGALWNS"
     gene            complement(5883..6722)
                     /locus_tag="PKM49_06020"
     CDS             complement(5883..6722)
                     /locus_tag="PKM49_06020"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNY82407.1"
                     /translation="MKLFISLDMEGIAGTFNWQQETTQDRALVRKWMAQQIEWVIEGI
                     RQSPKNAMVTEITLADSHSNGDNLAYDITGLDERLSLISGNPRPNYMMPTLDGSYDTV
                     FLIGYHAGAGTPYGNMDHTYSNSTIHSLWINGKAMNETFINSAYASCFSIPVSLIVGD
                     KALKDQVMVEGGMPWVEFVVTKEALFKFAAKQRPLECVREETSKAVQKALAKDFKKLP
                     LYGFQKPYELKIEFQTSNQADFASMIPLVERIDGRTLRFVSEDFKQIFEACIYLPALA
                     RQG"
     gene            complement(6734..7510)
                     /gene="dapF"
                     /locus_tag="PKM49_06025"
     CDS             complement(6734..7510)
                     /gene="dapF"
                     /locus_tag="PKM49_06025"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNY82408.1"
                     /translation="MICVRYAACGNTFTIVFGEVIEDKEKPKVVRSIVAEMDGVIFVE
                     KTESSFFMDYFNRDGSRGEMCGNGARAFIKALYDRGECALGQLLVFDTYSGILYGRML
                     AADRAEVLMPQPEFLSMFHHEGWKGAFYSVGVPHLAFFMRTVSGVDVEGYGYYFSHLR
                     DNSSGVSPFPVSTNVNFFSEEDEGLFVRTYERGVWRETASCGTGATACAAFYRKMTGQ
                     QSSAIRVTTKGGVLTVRFDRPHPQKEGEATYLEGTIEHYP"
     gene            complement(7507..8163)
                     /locus_tag="PKM49_06030"
     CDS             complement(7507..8163)
                     /locus_tag="PKM49_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNY82409.1"
                     /translation="MAFSFEKNLEYMLRITLAMLGGLAIGINREKLQRPAGLRTHALI
                     AVGSAFFALLSGEYFGGILGGDPGRIAAQIVTGIGFMGAGTIMRRGFTVTGLTTAATL
                     WVSASIGVGFGVGEFYLGGFMTLAVLLTLTVVKRLGLALSKNRTRFVIEMVYGKNGFQ
                     KVVDWLFENRITAGSLYYETDGERVTISISVDPDTVERLKQKWSELLNFKEIRSLEIK
                     "
BASE COUNT         2057 a         2333 c         2333 g         2057 t
ORIGIN      
        1 gcatagcatc gacaaccttc catacacacg ccggcctaaa aggttggcgt ttttttggtt
       61 atgggagcgc cgcgatgggc ctgtggggtt atcgcggggt aaccaacggg accccgcgat
      121 cctgacgaaa ctctgcgatc cagctgtaaa aaaaacgtat cgcgccgtcg caaaaagaag
      181 gagcgcttac gccgcgatgc cctgggcggt tatcgcgggg taaccaacgg gaccccgcga
      241 tcctgacgaa actctgcgat ccagctgtaa aaaaaacgta tcgcgccgtc gcgaaaagaa
      301 ggggcgctta cgccgcgatg ccctgggcgg ttatcgcggg gtaaccaaag ggctgacttc
      361 ggaaaaccac gaagtctcgc gatccgccct ttgtccttac tcaaaagcgt atcgcgccgt
      421 cgcgtaaaga agaggcgctt acgccgcgat gcttgttttc tctaaagtcg gtgttcccag
      481 gcgtgcccat ttccctccct accgtttcac cactttacga catctttcca caaccctcca
      541 caacccctta tagtcctcaa tcgagactat tctatcggca gaaaaatagt gtataatcca
      601 cgcaatggag ggattcggtg gcgtttagct tcgaaaaaaa cttagagtat atgttgcgta
      661 taactctcgc tatgctcggc gggttggcga tcggtatcaa ccgagagaag ctccaacgtc
      721 cggcgggctt gcggacgcac gccttgatcg ccgtggggtc cgctttcttc gccctgctct
      781 cgggcgagta ttttggtggt attttgggag gtgacccggg gcgaatagcc gcgcaaatcg
      841 ttactggcat agggtttatg ggagccggta ccataatgag acgcggattc accgtcacag
      901 gattgacgac cgcggcgacg ctatgggtat ccgcgtcgat cggcgtcggc ttcggggtgg
      961 gagaatttta cttaggcggt ttcatgactt tggccgtgct cttgacgctg accgtcgtga
     1021 aaagactcgg cttagccctc agtaaaaatc gcacgcgctt cgtcattgaa atggtctatg
     1081 ggaaaaatgg attccagaaa gttgtagact ggctttttga gaatagaatc accgcgggct
     1141 ctctgtacta tgaaaccgac ggcgagaggg tcacgatctc tatcagtgtc gatccggaca
     1201 ccgtggaaag attaaaacag aagtggagcg aacttctaaa cttcaaagaa attcgttccc
     1261 tcgaaatcaa atgatttgcg ttcgatacgc cgcttgcggc aacacgttca cgatcgtttt
     1321 cggagaagtg atcgaagaca aagaaaaacc gaaggtcgtc cgatcgattg tggccgagat
     1381 ggacggcgtc atctttgtcg aaaagacaga gtcttcgttc tttatggact acttcaaccg
     1441 tgacgggtcc cgcggtgaga tgtgtggcaa tggggcgcgc gccttcatca aagcgttgta
     1501 cgaccgcggt gaatgcgctc tcggacagct gctggtcttc gatacttaca gcgggatttt
     1561 atacggacgg atgctggcgg ccgacagggc tgaggtgttg atgccccaac cggaattctt
     1621 gtctatgttc caccacgaag gctggaaagg cgccttttac agtgtggggg ttccgcatct
     1681 ggcgtttttt atgcgaaccg ttagcggggt cgatgtggag ggatacgggt actatttcag
     1741 ccatctgaga gataattcat cgggggtatc gccctttccg gtgtccacca acgttaactt
     1801 tttttctgaa gaggatgagg gtctgttcgt ccgcacctac gagcgtgggg tatggcgcga
     1861 gacggcctct tgcggaaccg gcgctaccgc gtgcgccgct ttctatcgaa aaatgaccgg
     1921 acaacagtct agcgcaattc gggtaaccac aaaaggcggc gtcctcacgg tgcgcttcga
     1981 tcgccctcac ccacaaaagg aaggcgaagc cacgtattta gaaggcacga tagaacacta
     2041 tccctaaagg aggttgacat gaagttattc atctcactcg atatggaagg tatcgccgga
     2101 acctttaact ggcagcagga aacgactcag gatcgggcgc tcgtgagaaa atggatggcg
     2161 cagcagatag aatgggtcat cgagggcatt cggcaaagcc cgaaaaacgc gatggtcact
     2221 gagatcactt tggccgattc tcacagcaac ggagacaatc tcgcttatga catcaccggt
     2281 ttggacgaac gcttatcact gatctccggg aacccacgcc caaactatat gatgcctact
     2341 ctagatggca gttatgacac cgttttcttg atcggctatc acgccggagc cggaacccct
     2401 tatggcaata tggaccacac ctattcgaat tcgacgatac acagcctgtg gatcaacgga
     2461 aaagcgatga acgaaacctt catcaactcc gcctacgcct cttgtttttc aatccccgtc
     2521 tccttgatcg tgggggacaa agccctcaaa gaccaagtga tggtcgaagg agggatgcct
     2581 tgggtcgagt ttgtcgtgac aaaagaggcg ctcttcaaat tcgccgcaaa acaacgtcct
     2641 ctggagtgtg tacgagaaga aacgagcaag gcggtgcaaa aagccctcgc caaagatttt
     2701 aaaaagctcc ccttgtacgg cttccaaaaa ccctacgaat tgaaaataga gttccaaaca
     2761 tccaaccaag cggacttcgc ctctatgatc cctttggtcg aacgaatcga tgggcgcacc
     2821 cttcgcttcg tctccgaaga tttcaaacaa atcttcgaag cctgtatata cctccccgct
     2881 ctcgcgcggc agggataacg gtgaggatag gcgtcgtcag cccgtcctct ctgcctaaaa
     2941 gcgaacgact gtttaaaggg ctttctcttc tacgcgatca agagagccag ctgatcattg
     3001 gcaagagctg tttccattat ctctccccat tggagcgcgc cgaagaactc gagaagatgg
     3061 aggatatctg tgatctcctt ctatgctctc gtgggggttg cggcagtttc cagctggccc
     3121 cttttttgac taggggattc aagattccga tctgcggata cagcgatatc acctttttac
     3181 tcctgtggca gtacgctcac ggggaacggg cctttcacgg accgatgctc tgcgattttc
     3241 aacgaccgac agacatccag ccctcctttc tgcctttcct gcgaaacgat atcacgctgc
     3301 cctatcgtta ccccgggatg aaaagaactt ggccgctgcg cctaggccag gccgtcggac
     3361 ggttactggc agtcaaccta tcgctgctga tcgcgtgttt accctttttt ggagacaagc
     3421 ttctggatgg ctcggtacta ttcttggaag acgtttcgga atcggtggat tcgatcgagc
     3481 ggtacctgtg ggcgctaaga cattacccta cgttcgccag agtcaaaggg gtcgtgttct
     3541 cggctttaac cgaaacacgc cgcgcatcaa acgattatcg gctgcgtgat gtcttgacgc
     3601 ggtgggctca ggaagccggc attccctgct tcatcgggtt tcccgcatat cacgggaagt
     3661 acataaagca ctgtttaccc atcggcggaa ccgtcgagat cgacagcgag actgggaccg
     3721 tcacacttct atctttagga ggcgcgctat ggaattctta gctttaccct tacccgaaat
     3781 gatagtgagt ttagaagcgc agtatcggtg ggaagacgcc cttcgctgcc ttcggatatg
     3841 gcttcaaaaa gacctggacg ccgcgctcag aaggcgtctg gagtacgaga tcttccgaat
     3901 cgcgagattg caaaagtcgt atgttcacga tgaaaagacc gcctattcga tgttggagga
     3961 agcaatcgtg gacttttcga aagaagagtt cgaccgactc gtccgcgaac gagctttaga
     4021 ctgcatccgg aaagatgacg ggctctactt cgagaaacgg ttcttaccca atctgttttt
     4081 cgcattcccc gactacgtct cccgaagacg taccgtggag gaaagtcggg agaaggcgaa
     4141 aaaacatctc gaatcacggc tggacgctct tttaagaggt gaaaagccga tgcgttaccg
     4201 tgtgattgga cggattcaga agcgccttaa accctctgct ttagaagctc tcgaggggcg
     4261 gcatgggggt ttggaatgtt ggttgccttt cccgttggag gatctgcaac aaacgaagcc
     4321 ggcgctgatc gccgcaagcg cgcctgaata cacgctacct caccgctttt cggaaacgcg
     4381 cgcgatccat atggatcgcg cgcgtttccg aaaagcggtg aggtagcgtg tattcaggcg
     4441 cgcttgcggc gatcagcgcc ggcttcgttt gttgcagatc ctccaacggg aaaggcaacc
     4501 aacattccaa acccccatgc cgcccctcga gagcttctaa agcagagggt ttaaggcgct
     4561 tctgaatccg tccaatcaca cggtaacgca tcggcttttc acctcttaaa agagcgtcca
     4621 gccgtgattc gagatgtttt ttcgccttct cccgactttc ctccacggta cgtcttcggg
     4681 agacgtagtc ggggaatgcg aaaaacagat tgggtaagaa ccgtttctcg aagtagagcc
     4741 cgtcatcttt ccggatgcag tctaaagctc gttcgcggac gagtcggtcg aactcttctt
     4801 tcgaaaagtc cacgattgct tcctccaaca tcgaataggc ggtcttttca tcgtgaacat
     4861 acgacttttg caatctcgcg attcggaaga tctcgtactc cagacgcctt ctgagcgcgg
     4921 cgtccaggtc tttttgaagc catatccgaa ggcagcgaag ggcgtcttcc caccgatact
     4981 gcgcttctaa actcactatc atttcgggta agggtaaagc taagaattcc atagcgcgcc
     5041 tcctaaagat agaagtgtga cggtcccagt ctcgctgtcg atctcgacgg ttccgccgat
     5101 gggtaaacag tgctttatgt acttcccgtg atatgcggga aacccgatga agcagggaat
     5161 gccggcttcc tgagcccacc gcgtcaagac atcacgcagc cgataatcgt ttgatgcgcg
     5221 gcgtgtttcg gttaaagccg agaacacgac ccctttgact ctggcgaacg tagggtaatg
     5281 tcttagcgcc cacaggtacc gctcgatcga atccaccgat tccgaaacgt cttccaagaa
     5341 tagtaccgag ccatccagaa gcttgtctcc aaaaaagggt aaacacgcga tcagcagcga
     5401 taggttgact gccagtaacc gtccgacggc ctggcctagg cgcagcggcc aagttctttt
     5461 catcccgggg taacgatagg gcagcgtgat atcgtttcgc aggaaaggca gaaaggaggg
     5521 ctggatgtct gtcggtcgtt gaaaatcgca gagcatcggt ccgtgaaagg cccgttcccc
     5581 gtgagcgtac tgccacagga gtaaaaaggt gatatcgctg tatccgcaga tcggaatctt
     5641 gaatccccta gtcaaaaaag gggccagctg gaaactgccg caacccccac gagagcatag
     5701 aaggagatca cagatatcct ccatcttctc gagttcttcg gcgcgctcca atggggagag
     5761 ataatggaaa cagctcttgc caatgatcag ctggctctct tgatcgcgta gaagagaaag
     5821 ccctttaaac agtcgttcgc ttttaggcag agaggacggg ctgacgacgc ctatcctcac
     5881 cgttatccct gccgcgcgag agcggggagg tatatacagg cttcgaagat ttgtttgaaa
     5941 tcttcggaga cgaagcgaag ggtgcgccca tcgattcgtt cgaccaaagg gatcatagag
     6001 gcgaagtccg cttggttgga tgtttggaac tctattttca attcgtaggg tttttggaag
     6061 ccgtacaagg ggagcttttt aaaatctttg gcgagggctt tttgcaccgc cttgctcgtt
     6121 tcttctcgta cacactccag aggacgttgt tttgcggcga atttgaagag cgcctctttt
     6181 gtcacgacaa actcgaccca aggcatccct ccttcgacca tcacttggtc tttgagggct
     6241 ttgtccccca cgatcaagga gacggggatt gaaaaacaag aggcgtaggc ggagttgatg
     6301 aaggtttcgt tcatcgcttt tccgttgatc cacaggctgt gtatcgtcga attcgaatag
     6361 gtgtggtcca tattgccata aggggttccg gctccggcgt gatagccgat caagaaaacg
     6421 gtgtcataac tgccatctag agtaggcatc atatagtttg ggcgtgggtt cccggagatc
     6481 agtgataagc gttcgtccaa accggtgatg tcataagcga gattgtctcc gttgctgtga
     6541 gaatcggcca aagtgatctc agtgaccatc gcgtttttcg ggctttgccg aatgccctcg
     6601 atgacccatt ctatctgctg cgccatccat tttctcacga gcgcccgatc ctgagtcgtt
     6661 tcctgctgcc agttaaaggt tccggcgata ccttccatat cgagtgagat gaataacttc
     6721 atgtcaacct cctttaggga tagtgttcta tcgtgccttc taaatacgtg gcttcgcctt
     6781 ccttttgtgg gtgagggcga tcgaagcgca ccgtgaggac gccgcctttt gtggttaccc
     6841 gaattgcgct agactgttgt ccggtcattt ttcgatagaa agcggcgcac gcggtagcgc
     6901 cggttccgca agaggccgtc tcgcgccata ccccacgctc gtaggtgcgg acgaacagac
     6961 cctcatcctc ttcagaaaaa aagttaacgt tggtggacac cggaaagggc gatacccccg
     7021 atgaattatc tctcagatgg ctgaaatagt acccgtatcc ctccacatcg accccgctaa
     7081 cggttcgcat aaaaaacgcc agatgcggaa cccccacact gtaaaaggcg cctttccagc
     7141 cttcgtggtg gaacatagac aagaattccg gttggggcat caacacctca gccctgtcgg
     7201 ccgccagcat ccgtccgtat aaaatcccgc tgtaagtatc gaagaccagc agctgtccga
     7261 gagcgcattc accgcggtcg tacaacgctt tgatgaaggc gcgcgcccca ttgccacaca
     7321 tctcaccgcg ggacccgtca cggttgaagt agtccataaa gaacgaagac tctgtctttt
     7381 cgacaaagat gacgccgtcc atctcggcca caatcgatcg gacgaccttc ggtttttctt
     7441 tgtcttcgat cacttctccg aaaacgatcg tgaacgtgtt gccgcaagcg gcgtatcgaa
     7501 cgcaaatcat ttgatttcga gggaacgaat ttctttgaag tttagaagtt cgctccactt
     7561 ctgttttaat ctttccacgg tgtccggatc gacactgata gagatcgtga ccctctcgcc
     7621 gtcggtttca tagtacagag agcccgcggt gattctattc tcaaaaagcc agtctacaac
     7681 tttctggaat ccatttttcc catagaccat ttcaatgacg aagcgcgtgc gatttttact
     7741 gagggctaag ccgagtcttt tcacgacggt cagcgtcaag agcacggcca aagtcatgaa
     7801 accgcctaag taaaattctc ccaccccgaa gccgacgccg atcgacgcgg atacccatag
     7861 cgtcgccgcg gtcgtcaatc ctgtgacggt gaatccgcgt ctcattatgg taccggctcc
     7921 cataaaccct atgccagtaa cgatttgcgc ggctattcgc cccgggtcac ctcccaaaat
     7981 accaccaaaa tactcgcccg agagcagggc gaagaaagcg gaccccacgg cgatcaaggc
     8041 gtgcgtccgc aagcccgccg gacgttggag cttctctcgg ttgataccga tcgccaaccc
     8101 gccgagcata gcgagagtta tacgcaacat atactctaag tttttttcga agctaaacgc
     8161 caccgaatcc ctccattgcg tggattatac actatttttc tgccgataga atagtctcga
     8221 ttgaggacta taaggggttg tggagggttg tggaaagatg tcgtaaagtg gtgaaacggt
     8281 agggagggaa atgggcacgc ctgggaacac cgactttaga gaaaacaagc atcgcggcgt
     8341 aagcgcctct tctttacgcg acggcgcgat acgcttttga gtaaggacaa agggcggatc
     8401 gcgagacttc gtggttttcc gaagtcagcc ctttggttac cccgcgataa ccgcccaggg
     8461 catcgcggcg taagcgcccc ttcttttcgc gacggcgcga tacgtttttt ttacagctgg
     8521 atcgcagagt ttcgtcagga tcgcggggtc ccgttggtta ccccgcgata accgcccagg
     8581 gcatcgcggc gtaagcgctc cttctttttg cgacggcgcg atacgttttt tttacagctg
     8641 gatcgcagag tttcgtcagg atcgcggggt cccgttggtt accccgcgat aaccccacag
     8701 gcccatcgcg gcgctcccat aaccaaaaaa acgccaacct tttaggccgg cgtgtgtatg
     8761 gaaggttgtc gatgctatgc
//
LOCUS       DAOPXN010000086         8620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8194_length_8620_cov_5.839696, whole genome shotgun sequence.
ACCESSION   DAOPXN010000086 DAOPXN010000000
VERSION     DAOPXN010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8620
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8194_length_8620_cov_5.839696"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(610..1308)
                     /locus_tag="PKM49_06035"
     CDS             complement(610..1308)
                     /locus_tag="PKM49_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82410.1"
                     /translation="MKTAVIVCASLIFTISVVLGGWILLPGVPRPASYETINRKTILL
                     FEEVLSESPITYRSGHYTSLMQYSGSFSDILKPGAIILYKTNAGNYGKMKITGYAYYH
                     AWSKAQNYQYCDVPFFEFTTYDDSGSVLVTGGFPIILDSIVRVVDQSPLWGIGPVGPH
                     FLRDLRYANYRERPPDSCFMWFYFDFDTKQTGFFSDYDGIPFTGADVHNVKTETRSRF
                     IPTQGALFAVIYKP"
     gene            complement(1381..3282)
                     /locus_tag="PKM49_06040"
     CDS             complement(1381..3282)
                     /locus_tag="PKM49_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82411.1"
                     /translation="MKKALLVVLVLVFAMSLSFAAAGKVSFLVDKGVVTAGDPVKVTF
                     TLEETEVEEGLTTISVLGEQLTPNVWVMLVQGGNAQYEATKAVDEEGKKTVYYIRAME
                     LKGQKVTLYAKLTDWNGKVIKQEKKDVQWKEDAYSDVIALVPAAEGIYSIDASITLED
                     GTVIAMGAGQLVAMKKYVEQTIDGLKASLSPAQYSLTVKTGIEFMQKLGDQRDSDPHL
                     SIYGKGKEVIPSIVWSWAGRKQPAMGPDVELVYVPKAKTVLGKVDTAYIKFKVLSEAL
                     EGDCCGASAGDKWALWLAIGTGQYHGMYEASYYLTNCCTVFHYMGVTHYKDWYWGILK
                     GGDCRGWVKAIFYLDPADVKIWVYAIVGTTWYEGFDIGDDDWGTPSGQGTGNDTSQSL
                     YTANLLKNAELNIFADIIFKDIVTLKFMAINIPITAENLKPVIGASIGVDLGKIGLGA
                     AWRYDTAKELNESVWAAHLVLNELLNFTIPGGFPFEALNKIGFGFFAAGDLGGSTVPS
                     TTAVSFNVTHLQLILELGADITCCFPEIHIGLHLAKLPSSGTGSSNSSSGMLSGIGLT
                     EIWGKYSHSLGTYGPVTLKGILGVIYAFEGGYVDNGVTCSGWSGYGKGLYDGPAIGVA
                     YEVEGTLKW"
     gene            complement(3504..5669)
                     /locus_tag="PKM49_06045"
     CDS             complement(3504..5669)
                     /locus_tag="PKM49_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82412.1"
                     /translation="MKKGLSLLLIALLLFFTLGLGTEAVKPEDVKVLETLIELMSGKL
                     GEYQDQFKDFKGLLGNLSGKIGDIESKSEALNQMDRWLRTKIEDTEKSLRERITTGEE
                     KLQSAIEEKIQAVTQRLTGIDSGISNLKGEIEAIYQEIYRLGFELFEAVGESGQETTQ
                     RIAFIQSQIDLLYKKIKQLEGKDGSGASALDGISSDIALLKEIIGYVDQANVGLWNAA
                     SVREKEAEARYEEINLRLKSVENAFIALRSEDADTRNALDEMISGVGLLQQSVASLQA
                     NDKFLWNKVDIAERQLAAKVQAGDEQLAQLLTEKVSALNLRLKWVDGSIEKLKGDLKA
                     LDKDVLELSAGLERLRKEQASGNGNLSQVIDDRVWAINKRLGWTDGAVAKLKTDLESL
                     QKKLNEATSNLEEETLNRIWGVNKRLMWLENQVSQIKNESGQSQEIVGLSIANLELEV
                     QLLKTRTQTIELEKALKGSLDEMVVAKLNDVVGRCAQIEKQLNVMKTFATKDELAVTN
                     QSVAKIAKETAEKFPALDARLAWIDKTLASQKGQVENLARNLAEVKGSLSEELAERMW
                     AINTRLKWIDGAITKLKSQKADDADMNAKFDAIQGELLGLSDDILALAIAIQKNNEEI
                     AQTKVGLRETAAELTQRIDAQGADIQSQIASMRARLEVSISANQQAINGQGRKIDDLE
                     TRVKALEDSVFKKPENTQPAGIILLLAGVAALALLFANH"
     gene            complement(6140..7336)
                     /gene="tyrS"
                     /locus_tag="PKM49_06050"
     CDS             complement(6140..7336)
                     /gene="tyrS"
                     /locus_tag="PKM49_06050"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNY82413.1"
                     /translation="MPSEFAQLQLIKANCVDLYSEPELLERLKNGVPLRVKLGVDPSR
                     PDLHLGHAVVLRKLATFQNLGHQVVLVIGDFTARIGDPSGRSQTRPMLSAEEVGLYAK
                     SYEEQVFRVLDPVKTEIRFNGEWLDKLNFEKVIRLAAKYTIARMLERDDFAKRYASHE
                     PISVSEFLYPLAQAYDSVAIKADVELGGTDQLFNLLVGRKIQEEYGQPPQIVMTMPLI
                     EGTDGQMKMSKSYGNYIAFNDTPEDVYGKVMSIPDTLLLKYLRLLTDIDAETLTLYER
                     NIREGRVNPRDYKMELARRMVELLYDPEEAERAQEEFVRVFQKRDLPSEIPELLLDGA
                     SIETVDLLTKAGVSSRSEAKRLVQQGGVTLDDRKLEDPFAVLSLSESMILKIGKRKYY
                     RIVFDR"
     gene            complement(7355..7597)
                     /gene="secG"
                     /locus_tag="PKM49_06055"
     CDS             complement(7355..7597)
                     /gene="secG"
                     /locus_tag="PKM49_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNY82414.1"
                     /translation="MQAFKIIIYIVHALVSLGLIISVTLNMAKHTSLGGAFGSGANAT
                     VFGREKGLNRIGKITLWLAIAFMVLSFLTSYAMTRV"
     gene            complement(8203..>8620)
                     /locus_tag="PKM49_06060"
     CDS             complement(8203..>8620)
                     /locus_tag="PKM49_06060"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82415.1"
                     /translation="ASGRRDIEYLIELGIKYLLSSKPEVELEELVEIASKISEERGEQ
                     IMTLAERLIDKGMQKGREEGLKEGLKEGLEKGLASGVEKGKTDVIWKLMIKKFPNLAI
                     AYRDKIKQLDEGKLDVLALELLDMQKQEDLDRYLQT"
BASE COUNT         1837 a         2361 c         2149 g         2273 t
ORIGIN      
        1 tcaggtcttg ttctatgaag ctgtcctttt ccgcctgtat cgtgccgaca tcgatcgtct
       61 gtaagacatc tggcgggaga taggtctcta agaacgcttg ggtatttgcg acattcccca
      121 gtacttcttt gaaaaaccta tcgtgggggt tattcggtct ttccattttt gtcctcccaa
      181 cgggaaatca tttcacgggt attatacact aataaagagg gggaatgcgg atcgtagggc
      241 ttcatcagta tcgcgagact tcgtgggttt gcgaagttac cccgctggtt accccgcgat
      301 aacttgcacg ggcatcgcgg cgtaaagaaa acaaaagggg gatcgcgggg tcccttcggt
      361 taccccgcga ttaccccttg gcgcgatacc ctggttttcg ttcgttccaa agataaaaaa
      421 cagggttcgc acagagggca cagagaacac agagaaaaca gactacgcag gttaaaaaga
      481 acaaagatcg tatcgcgggg tcccttcggt taccccgcga taaccccgct cgggtgtcgc
      541 gagacttcgt ttcttcgcga agtcagcccg ttggttaccc cgcgatgacc tcacttaagg
      601 cgttatggtt tatggcttat atatcaccgc gaatagggcg ccctgggttg ggatgaagcg
      661 ggagcgcgtc tcggttttga cgttatggac gtcggcgccg gtgaagggga tgccgtcgta
      721 gtcgctgaag aatccggtct gtttcgtgtc gaagtcgaag tagaaccaca taaagcagga
      781 gtcgggcggg cgttcgcggt agttcgcgta gcgtaggtcg cgtaaaaagt gcgggcctac
      841 cggcccgatt ccccataaag gggactgatc tactacccgg acgatagagt cgaggattat
      901 ggggaagccg ccggttacga gtaccgagcc gctgtcgtcg taggtggtga actcgaagaa
      961 agggacgtca caatactggt agttctgggc tttcgaccat gcgtggtaat aggcgtaccc
     1021 ggtgatcttc atcttaccat aattcccggc gttcgttttg tagaggatga tggcgccggg
     1081 ttttaggatg tcggagaagc ttccggagta ttgcatcagg gacgtgtaat gtccggagcg
     1141 gtaagtgatc ggcgattcgc tgaggacctc ttcaaacaga aggatcgttt ttcggttgat
     1201 cgtttcgtac gaagcgggac gtggaactcc gggtagaagg atccacccgc cgagtactac
     1261 cgatatcgta aagataaggc tcgcgcaaac gatcacagca gttttcacct cgtatccctc
     1321 cctataaaaa aaccccgcct ttcggcgggg tctttatccg ttcggcttat gacagccttt
     1381 ttaccacttc aaagtacctt cgacttcgta ggcgactccg atggcgggac catcgtagag
     1441 tccttttccg taaccagacc aacctgagca ggtaactcca ttatcaacat acccgccttc
     1501 gaaggcgtag atcacgccga ggattccttt taaagtgacg ggtccatatg tgccgagcga
     1561 gtggctgtat ttaccccaga tttcggtcaa gccgattccg gagagcatac cggaagaact
     1621 attggaagag ccggtacccg aagacggaag cttggctaag tgcaatccga tatggatttc
     1681 cgggaagcag caggtgatat cggcgccgag ttccagaatc agctgcaggt gtgtcacgtt
     1741 aaagctgacc gcggtggtac tgggaactgt ggagccaccc aaatcgccgg ccgcgaagaa
     1801 cccgaatccg attttgttca gcgcctcgaa ggggaatccg cccgggatgg tgaaattgag
     1861 taattcattc aaaacgaggt gggctgccca tacgctttcg ttgagttctt tagccgtgtc
     1921 gtagcgccac gcggctccca agccgatttt tccaaggtct acgccgatcg acgcgccaat
     1981 cacaggcttc aaattttcgg cggtgattgg gatattgatg gccataaact tgagggtaac
     2041 aatgtctttg aagatgatat cggcgaagat gttcagttcg gcgttcttca acaggttcgc
     2101 ggtgtaaagg ctttgagaag tatcgttccc ggttccttgc ccgcttgggg tcccccagtc
     2161 atcatcacca atatcgaatc cttcatacca agtcgttccg acgatagcgt acacccagat
     2221 tttgacgtcc gcagggtcta aatagaaaat tgccttaacc cagccgcggc agtccccacc
     2281 tttgaggatt ccccagtacc aatctttgta atgagtgacg cccatgtagt ggaatacggt
     2341 gcagcagttc gtcaaataat aagacgcttc gtacatcccg tgatattggc cggtacctat
     2401 cgcaagccac agggcccatt tgtctccggc tgaagcgcca cagcaatcgc cctcaagggc
     2461 ttcgctgagc actttgaact tgatgtacgc ggtatcaact ttaccaagga ctgtttttgc
     2521 cttcggtacg taaaccaatt ccacatcagg acccatagcc ggttgttttc tcccggccca
     2581 agaccacact atcgacggga taacttcttt tcccttaccg tagatgctga gatgcgggtc
     2641 tgagtctctt tgatcgccca atttctgcat gaactcaata ccggttttta ccgttaaaga
     2701 atactgggcc ggcgataagg atgccttgag tccgtcaatc gtttgttcca catacttctt
     2761 catcgccacg agttgtccgg cgcccatcgc gatgacggtt ccgtcttcca gagtgatgct
     2821 cgcgtcaatg gagtagatcc cttctgcggc ggggactaaa gcgataacgt cgctgtaagc
     2881 atcctccttc cattgcacgt ctttcttctc ttgcttaata acttttccgt tccagtcagt
     2941 caatttcgcg tacagggtga ccttttgacc cttgagctcc attgcccgaa tgtagtacac
     3001 ggtcttcttt ccttcctcgt caaccgcttt ggtcgcttca tactgggcgt tgccaccctg
     3061 tacgagcata acccatacgt tcggagtcaa ttgttcgcca agtacggaga tcgtcgtcag
     3121 cccttcttcc acttcggttt cttccaacgt gaaggtcact ttgacgggat cgccggccgt
     3181 tacgacgcct ttgtcgacca gaaagctaac cttcccggca gcggcaaaag acaggctcat
     3241 tgcgaagact aagaccaata ctactaatag cgcttttttc atacgattac ccctccctct
     3301 cgatatagtt gttgtagtag ttttagatag gttctaccat tatgtaccgg gatagtatac
     3361 aacattttat ccgacttttg caacccccct ttcgatttcg tcgagaaatc cctttatagg
     3421 cccgtttggt ttccatcaca ccaccgccct catcccttcc tcaagcgcgc ttatgcctct
     3481 tgagtcgcgc cttacagggg agatcagtgg ttggcgaaga gtaaggccaa ggcggccacc
     3541 cccgccaaca agaggatgat cccggcaggc tgcgtattct ccggtttctt gaagacgctg
     3601 tcttccaagg ctttgaccct cgtttccaaa tcgtcgatct tccggccctg accgttgatc
     3661 gcctgttgat tcgccgatat cgaaacctcc aatctcgctc gcatcgaggc gatctgtgac
     3721 tggatatcgg cgccttgcgc gtctatcctt tgcgtcaact cggcagcggt ttcgcgtaat
     3781 ccgacctttg tctgcgcgat ctcttcgtta ttcttttgta tcgcgatggc cagcgcgagg
     3841 atgtcgtcgg aaagcccgag aagctcgccc tggattgcgt caaatttcgc gttcatatcg
     3901 gcgtcgtcgg ccttctgtga cttgagcttc gtgatcgccc cgtctatcca cttaagacgg
     3961 gtgttgatcg cccacatccg ctcggccagt tcctcggaga ggctgccctt tacttcggct
     4021 aaattcctcg ccaagttctc cacttgaccc ttttgagaag cgagcgtctt gtctatccaa
     4081 gcgagtcgcg catccaacgc cgggaatttt tcggccgtct ctttcgcgat cttcgccaca
     4141 ctctggttcg tgaccgcgag ttcgtctttc gtcgcaaacg tcttcatcac gttgagctgt
     4201 ttctctattt gcgcgcaccg gcccaccaca tcgttcaact tcgcaacgac catttcgtct
     4261 aaagaacctt taagggcttt ttctagttcg atcgtttggg ttcgcgtttt gagaagctga
     4321 acttccagct ccaagttggc gatcgataga cccacgatct cttgactctg accgctttcg
     4381 ttcttgattt gggagacctg gttttccaac cacatcaaac gcttgtttac accccaaatc
     4441 cggttgagcg tctcttcttc caaattcgat gtcgcctcgt tcaacttctt ttgaaggctt
     4501 tcgagatccg ttttcaactt tgcgaccgcg ccgtcagtcc aaccgagccg cttgtttatc
     4561 gcccagaccc gatcgtctat gacttgactc aaattaccgt tcccagaggc ttgttctttc
     4621 ctcaaccgct ctaatccagc gctgagttcg agcacgtctt tgtcgagggc tttcaggtca
     4681 cccttcagtt tttcgatgct cccgtcaacc catttcaacc tcaaattgag cgcgctcact
     4741 ttttcggtga gtagctgcgc gagttgttcg tcgcccgctt ggacctttgc ggccaactgc
     4801 ctttcggcga tgtcgacctt gttccaaagg aatttgtcgt tcgcctgtaa agaagcgacc
     4861 gactgttgga ggaggcccac tcccgaaatc atctcgtcca aggcgttgcg cgtatccgcg
     4921 tcttctgatc tcagcgcgat gaaggcgttt tccaccgatt tcagccgaag gttgatctcc
     4981 tcatagcgag cctcggcttc tttctccctc accgaagccg cgttccagag accgacgttc
     5041 gcttggtcca cgtatccgat tatctccttg agcaatgcga tatcggatga gatgccgtct
     5101 agggcagagg ccccgctccc gtctttcccc tccaattgct taatcttttt gtacaggaga
     5161 tcgatctgcg attggatgaa cgcgattctc tgcgtcgtct cctgacccga ctctcccacg
     5221 gcttcaaaga gttcgaaccc taaccggtag atttcctggt agatcgcttc gatctcgcct
     5281 ttcaggttcg agatgcccga gtcgatcccg gtcaatcgct gggtgacggc ctgtatcttt
     5341 tcttcgatcg ccgactgtag cttttcttcg cccgtggtga tccgttctct cagtgatttc
     5401 tccgtatctt cgattttcgt ccgtagccac cggtccatct ggttcaaggc ttcggattta
     5461 gattctatgt cgcctatttt cccggatagg ttgcccagta gtcctttgaa atctttaaac
     5521 tgatcctgat actcccccag ctttccactc atcaattcta tgagggtctc gaggactttg
     5581 acgtcttccg gttttaccgc ctcggtcccc aaacccagag tgaaaaagag gagcaacgcg
     5641 atcaacaata agctcaatcc ctttttcatg gtggtccccc tttctcctgg ttgccgtatt
     5701 tcccttttct agatcgcagt ccgtaaagat gacgcaaaca cctgtctctt cccttagtgt
     5761 agagttcagc gcgtaaagtc gaaacgaaaa aaaacgtgaa atgcctgatg tcacaaagaa
     5821 tagcttccta caggatatct taaaaaatac cttatcttcc tattttccat aaaagtatcg
     5881 cgcttttatc ggttgtccgt atagcaaaac accacaaaga acgatacgtt atccgagggt
     5941 atcgttttac tcctgctcat tcactttcca aagacccgac cccgtctctt gcgatggctt
     6001 cgttctcgga aattatcaac atatacccgt tttacgggtg atcgcgataa ttataccacc
     6061 ttcttcggaa aaatacaaaa aaacttcgca atttgacgaa tccggtcgat cacgcgtcac
     6121 aacgacgctc ccaaagccct taccgatcga acacgatgcg ataatacttc ctctttccga
     6181 tcttcaagat catgctttcc gatagggaaa ggacggcgaa gggatcttcc agttttcggt
     6241 cgtctaatgt gacgccgccc tgttggacca agcgcttcgc ttcgctgcgg gaggacaccc
     6301 ccgctttcgt gagtaaatcg acggtttcga tggatgcccc gtccaagagg agttcaggaa
     6361 tctcgctcgg caggtcccgt ttttggaaca cccgcacgaa ctcctcttga gccctttcag
     6421 cctcttccgg gtcgtagagg agctccacca tccggcgagc gagctccatc ttgtaatccc
     6481 gcgggttgac gcgtccctcg cggatattcc gctcgtaaag ggtgagggtt tcagcgtcta
     6541 tgtcggttaa gagtcgcagg tatttcaaca agagggtgtc gggaatcgac atcaccttcc
     6601 cgtagacgtc ttcgggggtg tcgttgaagg cgatgtagtt cccatagctc ttgctcatct
     6661 tcatctgtcc gtcagtacct tcgatcaacg gcatcgtcat gacgatttgg ggtggctgac
     6721 cgtactcctc ttggatcttt cttcccacca aaaggttgaa gagctgatcg gtcccgccga
     6781 gttccacgtc ggctttgatt gcgacggagt cgtaggcttg ggccaaaggg tagaggaatt
     6841 cggagacgct gatcggctcg tgggaggcgt agcgcttcgc gaaatcgtct cgctcgagca
     6901 tacgcgcgat cgtgtacttc gcggcgagtc gtatgacctt ctcgaagttt agcttgtcaa
     6961 gccattcgcc gttgaaccgg atctccgttt tcacggggtc gaggacacga aagacctgtt
     7021 cttcatagga cttcgcgtac aggccgacct cttcggcgga cagcatcggc ctcgtctggg
     7081 aacgcccgga agggtcgccg atccgtgcgg tgaagtcccc gatcaccagg accacttgat
     7141 ggcccaggtt ttgaaaggtc gccagttttc gcaacaccac ggcgtgtccc aagtgcaagt
     7201 ccgggcggga tgggtccacg cccaatttca cgcgcaaggg gacgccgttt ttaaggcgtt
     7261 ccaagagctc cggttcggag tagaggtcga cgcagttggc cttaatcagt tggagctgag
     7321 cgaactcgga cggcatagtc gagggcgttt cggcttaaac ccgggtcatg gcgtaagagg
     7381 tcaagaagct gagcaccatg aaagcgatgg ccagccagag ggtgatcttg ccgatcctgt
     7441 tcagcccttt ctcccttccg aataccgtgg cattggctcc cgaaccgaat gcgccgccca
     7501 acgacgtgtg tttcgccata ttgagggtga cggatatgat gagtcctaat gacacgagcg
     7561 cgtgtacgat atagattata atcttgaaag cctgcatcgt gcgggtcctc cttatagtag
     7621 gataaatcag cctattatac acaagagagc tggaaaaatc aaggatatgc ggaaggtatg
     7681 ccgggcgtcc gggaacgtgt gaaaaagaag aagaagggtt cgctgggaat gcctgggaac
     7741 gctaactttt aagttggtat gttttagatt ttcgggaagt cagcccggcg taagaggagc
     7801 aaaatgggga tcgcggggtc ccgttggtta ccccgcgata accccgcttg ggcatcgccg
     7861 cgtaagcgct tcttcttttt gcgacggcgc gataccccaa catcatcgcg gcgtaagagg
     7921 agcaaaatgg ggatcgcggg gtcccttcgg ttaccccgcg atgaccccgt ggcgcgatac
     7981 gcttttgaac atgtcttgaa aaacagggtt cgcacagagg gcacagagaa cacagagaaa
     8041 aaaatggcaa agggtgggga tcgcggggtc gtaaaaacca cttaccccgc gataaccccg
     8101 cttgggcatc gcggcgtaag cgcttcttct ttttgcgacg gcgcgatacg gttttaaagc
     8161 aagaacatgc cggactaaag tccggcgttc ccaggcgctc gctcaggttt gtaagtaacg
     8221 gtccaggtcc tcttgttttt gcatatcaag caactccaac gcgagtacgt caagcttacc
     8281 ctcatccagc tgctttattt tgtcacggta ggctattgca aggtttggaa acttttttat
     8341 catcagtttc caaattacat cagttttccc tttttcaacg ccactcgcta agcctttttc
     8401 taatccttct ttcaaccctt ctttcaaccc ttcttctcgg cccttctgca ttcctttgtc
     8461 gatgagtctc tccgcaagcg tcatgatctg ttcacccctt tcttccgaga tcttgctcgc
     8521 gatttcgacg agctcttcta actctacctc tggtttcgag cttaagagat actttattcc
     8581 cagttcgatc aagtattcta tgtcccttct acctgacgcc
//
LOCUS       DAOPXN010000087         8571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8249_length_8571_cov_4.716416, whole genome shotgun sequence.
ACCESSION   DAOPXN010000087 DAOPXN010000000
VERSION     DAOPXN010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8249_length_8571_cov_4.716416"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PKM49_06065"
     CDS             <1..196
                     /locus_tag="PKM49_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82416.1"
                     /translation="IGLGNVSVVSWGTMLYWAQANGALLAGFWWWFLPPGICIALIGT
                     SFALMNFAIDEISNPKLRKR"
     gene            202..1170
                     /locus_tag="PKM49_06070"
     CDS             202..1170
                     /locus_tag="PKM49_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013129564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82417.1"
                     /translation="MKETLPESILECQDLVAGYELKAGFVRAVDGVSFALKRGEFLGI
                     AGESGCGKTTLAYAVMRLFRDNAKIKSGKILFGGKDITHAPEEEIKKLRWEHISMVFQ
                     SAMNALNPVLQIGEQLTDGWLAHKRGEVREAKARALEVLKLVDIPEDRYNAYPHQLSG
                     GMKQRVMIAMALMLQPEVIILDEPTTALDVVVQRTIIEKIQELRKTFRFSVIFITHDL
                     SLLVEISDLLAIMYAGKIVEYGPASEVFYNPVHPYTRGLMASFPTLSGPIVRMGGIEG
                     KPPDLIDPPSGCRFYPRCPDRHDGCDRDTPPLRQVRPGHFAACDGE"
     gene            1172..2182
                     /locus_tag="PKM49_06075"
     CDS             1172..2182
                     /locus_tag="PKM49_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82418.1"
                     /translation="MTEEILRLESVSKHFPIRSFRRFGEVVHAMDDVSFILGKGEVLA
                     LVGESGSGKTTTASVIAQLYPITSGVIRLADPRSPNGFQSVSRKLSGKELLSYRKRVQ
                     MIFQDPFGSLNPTHTIEHILSRPYLIHDKVVRENEGEKRKAALKRQLQQLLRSVGLEP
                     PQQFLDKFPHELSGGQRQRVNIARTFSIEPEIVLADEPTSMLDVSIRISIMNMMLAFQ
                     EERQMSFLYITHDLAGARYMAQRIVVMYAGMIMEIGTSDEVVQRSAHPYTKLLRSAAP
                     APEEGLMRKRLITQGDIPSLIHPPSGCRFHPRCPYVMAQCSKTIPPLKEVESGHSIRC
                     FL"
     gene            complement(2177..3781)
                     /locus_tag="PKM49_06080"
     CDS             complement(2177..3781)
                     /locus_tag="PKM49_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNY82419.1"
                     /translation="MKEKKHNELSEKPSIQGFNERISVEGLLDRIFELIRNASISAFY
                     RSLSDHRIVYANAYFSKTFRLPLGEWEKTPYLWTDFVAPVDKESYRQYFEGLKTPQYS
                     LDYRLIDELGQPRWVQEEGTVLFPKHSEGAMALALIHDISEHKRIEAELYGNEERLNR
                     VLTDLHVGLWDWDIPNKRYFGNGFWTQMFGFQPGTFTTDEAAWAAIVHPDDFDHFLLA
                     LEDPLTGKADQFSTEYRVRTLSGDWKWILDQGKVIDRDPLGRPVRLIGTIVDITAQKE
                     RQTELMHAKVKAEKASREKSELLASVNHDLKTPLTVILGAVETLLQESTAPTKPLLEM
                     IHSNTRRLEKMVGALLEIHQIELGMPTSLAKAIRTKRLFEDCNRNYTQLAKKKNLSYE
                     QRIAQDIPKTIILDQDRFLKVLNNLVDNAVKYTREGSVRLEVGYIPVEAGNARGNRAE
                     GELHVSVSDTGIGIPKAYLPYVFDRFFRVPNKGGRGRSPGFGLGLSICRDLVEQMGGA
                     ISIKPAEKGGTTVEFYVRVKEGNGAS"
     gene            complement(3866..5212)
                     /locus_tag="PKM49_06085"
     CDS             complement(3866..5212)
                     /locus_tag="PKM49_06085"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNY82420.1"
                     /translation="MKNTKLIALILLLCLCTFSFSVEVYLLENKTPPEKYKTLGDILY
                     VHAVTLLDAIGARYEWNNASKTLVVNWNQNSILFSNNSQTVLINNSPAYISNEPLILD
                     NRFFLPLLETCKLFGLRVVTKEGQVHVFSEISYLLYYFSIPDGYAFNFQLPVNFVISE
                     QSEERLLLDVYGSMTNRDWFLTNARLGIDRIQVANSTQDTLKAFIRFEIVTKPDRQAV
                     VTQENGTLWIRTQNRVIPEIVVNTKNKIVIDPGHGGIDPGAIGNRGTYEKNVVLSLSK
                     AVKSYLEKNDFIVYLTRENDVFVDLKQRAIFANNKGADLFVSLHLNSFEDISVQGVEV
                     YYYPWSEESYKIRLNRLYGALSEETVKAKVQRKLLSTNQSKKYAENIRDTLQKSGLSI
                     RKVIPGDFAVIAYTEMPAVLIECGYISNSNFERDLQKPEVLNLLAENIAKSIQNLF"
     gene            6419..6925
                     /locus_tag="PKM49_06090"
     CDS             6419..6925
                     /locus_tag="PKM49_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNY82421.1"
                     /translation="MIVGYKGIKPRIHPSVFIAPTAVIVGDVEIDEGSSVWFGAVVRG
                     DMSGVKIGKYSNIQDNCTIHTEADHPVTVGDHVTVGHNAIVHGCVIEDAALIGIGAIL
                     LNGAVVRTGATVAAGSVVRENQLVEERQLVAGIPAAVKKLQAEETTEHHRKHARKYAE
                     LAKVYVEN"
     gene            6948..7226
                     /locus_tag="PKM49_06095"
     CDS             6948..7226
                     /locus_tag="PKM49_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82422.1"
                     /translation="MVSATKEIIEKEMTISDIEKRFHVSTQRAVQLFCQAAKVSSPNT
                     SQLDAWSKQIIRRVHAKAKQSAQNREETCDSDCCDTSGGSCDQCRGCH"
     gene            complement(7230..>8571)
                     /locus_tag="PKM49_06100"
     CDS             complement(7230..>8571)
                     /locus_tag="PKM49_06100"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNY82423.1"
                     /translation="FSEPRPTPQLSFSVRELRTIAGVVVTASHNPKEYNGFKVYWEEG
                     SQILDAIADRITAHYLGLQDYLSLKGLPFEKGVSVGLIDVLSSEMDERYRRYVASCSL
                     NADVDKMLSMVYTPLHGTGAAHLIPLLNEQGYKQIETVAEQLTPDPDFSTAPYPNPED
                     PKAFALSIEKGKACDAELLLATDPDADRLGAMVRTPEGEYKPLTGNQMGAILVHYILD
                     SLSKTRGIPEKSVIVTSIVTSDFGPTVARTFGIPTVYTLTGFKNICGKANEYDRTGER
                     QFLFGYEESIGFVYGTRVRDKDAVSSGLLLTEAAAYHKKAGYSLYEWLQELFRRYGHH
                     EERMINYVLEGKEGAERIGRIMNRFRSVPLTEIGDAHLTQRIDYLEEPPEPALKTNCL
                     KFLYGENVWFALRPSGTEPKLKVYLYARSETRDSSSKIADLFYERVYSELKRIP"
BASE COUNT         1906 a         2235 c         2372 g         2058 t
ORIGIN      
        1 tatcgggtta ggtaacgtct cggtcgtcag ctgggggacg atgctgtatt gggcgcaagc
       61 caacggcgcg ctcttggcgg gcttctggtg gtggtttctt cctcccggta tctgtatcgc
      121 tctgatcggt acctccttcg cgttgatgaa cttcgcgatc gatgagatat cgaatcccaa
      181 actcagaaag agatgagcgc tatgaaagag actctacctg aaagcatatt ggaatgccag
      241 gacctggtag ccggatacga gttgaaagcc ggcttcgtcc gggccgtgga tggggtttcc
      301 ttcgcgctga agcgaggaga gttcctgggg atcgcgggcg aatccggttg cggaaaaacg
      361 acgctggcgt atgcggtgat gcgcttgttt cgcgataacg cgaagattaa aagcggaaag
      421 atactctttg gcgggaagga tatcacccac gcccccgagg aagagatcaa aaagttgcgc
      481 tgggaacaca tctctatggt cttccaatcc gcgatgaacg cgctcaaccc cgttctgcag
      541 atcggtgagc aactcaccga cggttggctc gcccataagc gcggcgaggt ccgcgaggcc
      601 aaagcgcgag cgttggaggt tctgaaactg gtcgatatcc ccgaagaccg gtacaacgcg
      661 tacccgcacc agctctccgg agggatgaaa cagagggtga tgatcgcgat ggcgctgatg
      721 ttgcaacctg aggtcatcat tttagatgaa cccacgaccg cgttggatgt tgtcgttcaa
      781 cggaccatca tcgaaaagat ccaggagttg cggaaaacct tccggttttc ggtaatcttc
      841 atcacccacg acctttctct tttggtggag atcagcgacc tgctggcgat tatgtacgcg
      901 gggaagatcg ttgaatatgg gcccgcctct gaggtgtttt ataatcccgt tcatccctat
      961 accagaggat taatggcctc tttccccacg ctgagcgggc cgatcgtgcg tatgggtggc
     1021 atcgagggca agccgccgga cctgatcgat ccgccttcgg gttgtcggtt ctatcctcga
     1081 tgtccagaca gacacgacgg atgcgatcgt gacacgcccc cgctccggca agtcagaccc
     1141 ggccatttcg cggcttgcga cggtgaatga tatgactgaa gagatattga gattggagag
     1201 cgtatcaaaa cattttccta ttcgctcttt ccggcggttc ggagaggtcg tccacgcgat
     1261 ggacgatgtc tccttcattc tgggaaaggg ggaggtactt gccttagtgg gcgaatcggg
     1321 cagcggaaaa acgacgacgg ccagcgtgat cgcgcagctg taccccatca cctccggcgt
     1381 gatccgattg gcggaccccc ggtctcccaa tggcttccaa tccgtgagcc ggaaactctc
     1441 tgggaaggag ttgctcagtt accgcaaacg ggtacaaatg atctttcagg acccgttcgg
     1501 ctccctcaat ccgacgcaca cgatcgaaca catcctgtct cgcccttact taatccatga
     1561 taaagtggtt cgcgaaaacg aaggagagaa acggaaagcc gcactaaaaa ggcagttgca
     1621 gcagctcttg cgtagtgttg gactggagcc tccccagcag tttttggata agttcccgca
     1681 cgagttatcc ggcggccagc gtcaacgtgt gaacatcgcc cgtacctttt ccatagaacc
     1741 ggagatcgtt ctggctgacg agcccacgtc gatgttggat gtgtcgattc gcatcagcat
     1801 aatgaatatg atgctggcct tccaagaaga gaggcagatg agctttttgt acatcaccca
     1861 cgacctggcc ggggcgcgct atatggctca acggatcgtc gtgatgtatg cggggatgat
     1921 tatggagatc ggcacctccg acgaggtggt acagaggtcc gctcaccctt acacgaaact
     1981 gcttcgttca gcagcgccag cgcccgaaga ggggttgatg aggaagcggc tgatcactca
     2041 gggagatatt ccgtcgttga tccatccgcc ctctggctgc cgttttcatc cgcgctgccc
     2101 ctatgtgatg gcccagtgct caaagacgat acccccgctc aaagaagtcg aaagcggcca
     2161 ttcgattcgg tgtttcctat gacgccccgt ttccttcttt gacgcgaacg taaaactcaa
     2221 cggtagttcc gcccttttcc gccggtttga tggaaatcgc gccccccatc tgttccacga
     2281 ggtcgcggca gatagacagc cctaagccga agccgggaga gcgaccacga ccgcctttgt
     2341 ttggtacccg gaaaaaacgg tcaaagacat acgggagata ggctttggga ataccgatcc
     2401 cggtatccga aacggacaca tgtagctcgc cctccgcgcg gtttcctcga gcgttgccgg
     2461 cttctacagg gatatagcca acctccagcc gaacggaccc ttcgcgggtg tacttcaccg
     2521 cgttgtcgac gagattgttc aagactttta aaaatcggtc ctgatcgaga atgatcgttt
     2581 tcgggatatc ttgggcgatc cgctgctcgt aggaaaggtt tttctttttg gccagctggg
     2641 tatagttcct gttgcagtcc tcgaatagcc gtttcgttct gatggctttt gccagtgagg
     2701 taggcatccc caattcgatt tggtggattt ctagaagagc tccgaccatc ttttcgagtc
     2761 ttctggtgtt gctgtgtatc atttccagaa gcggtttcgt cggggccgta ctttcttgca
     2821 gcaacgtttc gacagcgcct aagataaccg tgagcggcgt tttaaggtcg tgattcacgc
     2881 tggctagaag ctcggatttt tcgcgactcg ccttttcggc tttaaccttg gcgtgcatca
     2941 attccgtttg tctctctttt tgggcggtaa tatcgacgat cgtaccgatc aatctcacgg
     3001 ggcggcctaa agggtcgcgg tcgatgactt tcccttggtc caaaatccac ttccagtccc
     3061 cgctgagtgt tcgcacgcgg tattccgttg agaattgatc cgctttcccg gtcagggggt
     3121 cttccagcgc gagtagaaag tggtcgaaat catccggatg tacgatcgcc gcccaagcgg
     3181 cctcgtcagt cgtaaaggtt cccggttgga atccaaacat ctgggtccag aacccgtttc
     3241 caaaatacct cttgttcggg atatcccaat cccaaaggcc cacgtgaagg tccgtcaaaa
     3301 cacggttcag tcgctcttcg ttaccgtata gttcggcctc tatcctcttg tgttccgaga
     3361 tatcgtggat gagcgcgagc gccatcgctc cttcagagtg cttcgggaat agaactgtcc
     3421 cttcctcctg aacccacctc ggctgcccga gttcgtcgat caagcgatag tcgagcgagt
     3481 actgaggcgt tttcagccct tcgaaatact gccgataact ttctttatct acgggggcaa
     3541 cgaaatccgt ccataagtag ggcgttttct cccactctcc gaggggaagc cgaaaggtct
     3601 tagaaaaata ggcgtttgca tagacgatgc ggtgatcaga gagagagcga tagaatgccg
     3661 aaatagaggc gtttctgatc agctcgaaga tcctgtcgag cagtccttct acggatatcc
     3721 gttcgttgaa accctggatg gagggtttct ctgaaagctc gttgtgcttt ttttctttca
     3781 tcctgtgccc tcttttcctg acaacttgtt ttctcgaacc ccgacgagcc gctcgccccg
     3841 gtacaggcct ggctatcgag ccagcttaaa agaggttttg aatcgatttg gcgatgtttt
     3901 ccgccaataa gttcagtact tcgggttttt gaagatccct ttcaaaattc gagttcgaga
     3961 tgtagccgca ttcgatcaaa accgccggca tttccgtgta cgcgatgaca gcaaagtcgc
     4021 cgggtatcac cttacgaatc gatagcccgg acttctgtag tgtgtcgcgg atgttttccg
     4081 catacttctt gctctggttg gtcgacaaca gctttctctg cacctttgcc ttgaccgttt
     4141 cttcggataa ggccccatac agtcgattta gacgtatttt gtaactttct tccgaccaag
     4201 ggtagtaata gacctcgacg ccctgcacgg agatatcttc gaacgagttc agatgcagac
     4261 tgacgaaaag gtcggcccct ttgttgttgg caaaaatcgc ccgctgtttg aggtcgacga
     4321 agacatcgtt ttcacgggtc aggtacacga tgaaatcgtt tttctccaag tacgacttta
     4381 ctgctttcga caaagataag acgacgttct tttcgtaggt gccccggtta ccgattgcgc
     4441 cggggtcgat accgccgtgc cccgggtcga tcacgatctt atttttcgtg ttgaccacga
     4501 tctccgggat cacccggttt tgtgtccgaa tccagagcgt cccgttctct tgggttacca
     4561 ccgcctgccg gtccggtttc gtgacgatct cgaaacggat gaaggccttg agcgtgtctt
     4621 gtgtgctatt cgcgacctgt atgcgatcga tccctaggcg agcgttggtt aaaaaccaat
     4681 cccggttcgt catagatccg tagacatcca gtagaagccg ctcctcactc tgttcagaga
     4741 tgacgaagtt gaccggtagc tggaaattga aagcgtatcc gtccggtata ctaaaatagt
     4801 agagcagata agagatttcc gaaaacacgt ggacttggcc ttctttggtg acaacacgta
     4861 agccgaacag cttgcacgtc tccaagaggg gaagaaagaa acggttgtcc aaaatgagcg
     4921 gctcgttgga tatgtacgcg ggcgagttgt tgatcaacac cgtttgggag ttattggaaa
     4981 agagtatcga gttttggttc caattcacca ccagcgtctt actggcgttg ttccactcgt
     5041 atcgcgcgcc tatggcgtcg aggagcgtga ccgcgtggac atacaggatg tctcccaaag
     5101 ttttgtattt ttcgggcgga gttttgttct ccagcagata cacttccacg gaaaaggaaa
     5161 atgtgcaaag gcagagaagg aggatcagcg cgatcaactt tgtgtttttc atggtcactt
     5221 ccatttcgat tggaatagat ttctttgacg agagtgactc gacgtttctt cgcgaattat
     5281 accatagatc gttacgttga gtgcgatacg gcttggcatt tggatacgcc gaactttaat
     5341 ccggcatgtc tttaaaaacc aaaagatagt ctcgcacaga gtacacagag ggcacggagg
     5401 aaagattctt gggaactgcg gacttaacag gttatcgcgg ggtaagcggt ttttacgacc
     5461 ccgcgatccg ctctttgtct ttcccaaaag ggtatcgcgc tgtcgcaaga agaagggtcg
     5521 cttacgccgc gatgtccgtg gggttatcgc ggggtaacca acgggacccc gcgatccccc
     5581 tatgcactga gcgcttacgc cgcgatgttg tgcttgggaa cgccaacttt taagttggcg
     5641 tgtctttcca aaaccaaaag atagcctcgc acagagggca cggagagcac agagagaacg
     5701 ctgagataca tcctggtaac gtcgaacttt ttaggttatc gcggggtaag tggtttttac
     5761 gctcccgcga tccgcctttt gtctttctga aagcgtatcg cggcgtcgcg agaagaatgg
     5821 tcgcttacgc cgcgatgtcc gtggggttat cgcggggtaa ccaacgggac cccgcgatcc
     5881 cctatgcact gagcgcttac gccgcgatgc tacgcttggg aacgcctggg aacgccaact
     5941 tttaagttgg catgcattga taaaaggttt cgcacggaga gaacacgaca catgccacac
     6001 taaagtgtgg cgttcccagc aaagaaaaaa aataaaacta tgccgcacta aagttcggcg
     6061 tttccagcaa agaaaaatct atgaccctgc cacactaaag tgtggcgttc ccagggggct
     6121 ctcaaaagcc tggcgctccc aggcgttctc agtgttctct gtgcgaaccc tttttctttt
     6181 ttctaaaaac cgtgccaaac tgaagtttag cgttcccagc gcgttcgcag gcgctcccag
     6241 aaacccgcgc cgcactgaag ttcgacatcg ttaaaagaaa gcggtactgt cgactggtcc
     6301 gagttacggt tcccaccggc agttcatacc cccagactgc acccgcacgg ttgcgtaagg
     6361 gttcgtcgtt attgtataat accgggcata ccgtatgaag agatcggagg gtgagaggat
     6421 gatcgttggc tataaaggaa taaaaccgag gatccatccg tctgtcttta ttgcgccgac
     6481 ggccgttatc gtcggagacg tcgagatcga cgaaggaagc agcgtttggt ttggggcagt
     6541 cgtgcgtggg gatatgtcgg gtgtgaagat cggcaaatac tcgaatatcc aagacaattg
     6601 caccatccac acggaagctg accatccggt gacggtggga gaccacgtca ccgtggggca
     6661 taacgcgatc gtgcacggtt gcgtcatcga ggacgcggcc ttgatcggaa taggggcgat
     6721 cttgctcaat ggggccgttg ttcggactgg cgcgacggtg gccgcgggct cggtcgtgag
     6781 agaaaaccag ttggtggagg agcggcaatt ggtggccggt atccccgccg cggtgaagaa
     6841 gctccaagcc gaggagacga ccgagcacca tcggaaacac gcgcgaaagt acgccgaact
     6901 ggcgaaggtt tatgtagaaa attaacaatc gataaaagga ggacgctatg gtatccgcaa
     6961 cgaaagagat catcgagaag gagatgacga tctccgatat agaaaaacgg tttcacgtga
     7021 gcacgcaaag ggcagttcaa ttgttttgtc aagccgcgaa ggtgtccagt ccaaacacca
     7081 gtcaattgga cgcttggagc aaacagatca tccgacgggt tcacgcgaaa gccaaacagt
     7141 cggcccagaa ccgagaagaa acctgcgact ccgattgttg tgacacgtcc ggcggatctt
     7201 gcgatcagtg ccggggatgc cactaatctt caaggaatgc gcttaagctc cgagtaaacg
     7261 cgctcataga aaagatcggc tattttcgaa gaactgtcac gagtttctga tcgggcatag
     7321 aggtagactt tcagcttcgg ttccgttccg gagggtcgta aagcgaacca aacgttttcc
     7381 ccatagagga acttcaaaca gttggttttt agggccggct cgggcggttc ttccaaataa
     7441 tcgatccgtt gggtcaaatg cgcgtctccg atttcggtca agggtaccga ccggaatcgg
     7501 ttcataatgc gccctatcct ttccgcgcct tcctttcctt cgagaacgta gttgatcatc
     7561 cgctcttcgt gatgcccata ccgcctgaag agctcctgca accattcgta tagggaatac
     7621 cccgcctttt tgtggtaggc agccgcttcg gttaacagca gcccggaact gacggcgtct
     7681 ttgtcccgga ctcgggtgcc gtagacgaag ccgatgctct cttcgtaacc gaacaaaaac
     7741 tggcgctctc cggttcggtc gtattcgtta gctttcccgc agatattttt aaatcccgtg
     7801 agggtgtaga ccgtgggaat gccgaaagtg cgcgcgaccg tcgggccgaa atcagaggtg
     7861 acgatcgacg tgacgataac gctcttttcg ggtatccctc gcgtttttga gaggctatcc
     7921 aggatatagt gcaccaggat cgcgcccatc tggttcccgg tcaatggctt gtactcgcct
     7981 tcgggcgttc ggaccatcgc gcctaaccgg tccgcgtcag ggtcagtcgc gagcaagagt
     8041 tcggcatcac acgctttccc tttctctatg ctcaacgcga aggccttcgg gtcctccgga
     8101 ttcgggtagg gcgcggtcga gaagtccggg tccggggtga gctgttcggc gaccgtttcg
     8161 atttgcttgt acccctgttc gttgagtagg gggatcaggt gggccgcgcc ggttccgtgc
     8221 agcggggtgt agaccatcga taacatcttg tccacatccg cgttgaggga gcacgaggcc
     8281 acgtaccgtc ggtatcgttc gtccatttcc gacgagagca cgtcgatcaa accgacggat
     8341 acgcctttct cgaacggaag gcctttcaag gagaggtagt cctgtagccc caaataatgg
     8401 gcggtgatgc gatcggcgat ggcgtcgagt atctgagagc cttcttccca gtagacctta
     8461 aacccgttgt actctttcgg attgtggctg gccgtcacaa cgacaccggc gatggtccgc
     8521 aattccctca cagaaaacga gagctgcggg gtcggtctcg gttcgctgaa c
//
LOCUS       DAOPXN010000088         8560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8255_length_8560_cov_4.620223, whole genome shotgun sequence.
ACCESSION   DAOPXN010000088 DAOPXN010000000
VERSION     DAOPXN010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8255_length_8560_cov_4.620223"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..897
                     /locus_tag="PKM49_06105"
     CDS             172..897
                     /locus_tag="PKM49_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82424.1"
                     /translation="MTGLEKNLRRLFRPNGKTLWVALDHPQFCGPMPGLENPIQTIGR
                     ILLAPHDGMILNPGIFRLLPTEAYQKRLVMRVSVGGTCFSDYSKVHPVVVDPVQALCE
                     GADAVIMMLILGSQDALAMESVRRHIEAYHRYAIPVVVEVMSEDYTLNNDFQHVYAGA
                     RIAAELGCDIVKVFYCEGFHRVVETCPVPVLLAGGPKDADVLEGAKEALRSGAKGFAF
                     GRNVFQNENPVEMIDRLSRLLEG"
     gene            894..1865
                     /locus_tag="PKM49_06110"
     CDS             894..1865
                     /locus_tag="PKM49_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HNY82425.1"
                     /translation="MTIPKRIPNHLLYTIAVDYYLSGATQEEIGEKYGISRPQISRYL
                     RKAREEKIVEITVHPPTEIDQVALERGLEQKYGLKKAVVVPFLRRKYPPEELIRTIAI
                     SAAKTVKKLISIHQRIGIGWGKTVYQTVLAICSDRSGERPKPLMKRELAFYPLIGGLG
                     QSLPYYQVNTMIDRMAEYYHAKSRFFSVPAMTLKGSMLPTAMREDYESIVEVWKSVDL
                     AIIGLGGPIQQSEILKSEIDKDTIQRLIEQRAIGDILGRFFDERGVTCPTDLDSRLLG
                     LDLERLKSIKDVVCVCGGKNKVRAIQVALEHGFFNHLITDQQTASEL"
     gene            1879..2874
                     /locus_tag="PKM49_06115"
     CDS             1879..2874
                     /locus_tag="PKM49_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNY82426.1"
                     /translation="MKALIYSGPGQLNIQEIPAPQGKTIVEVGYAGICGTDVKTFLKG
                     HPMFKPPTVLGHECIGRIARTDAPERDLAEGMLVAVAPYAECGVCENCLKGYPELCRQ
                     KSFASSGSFCELLGMDTEYANRALFVIPDAQPVYTLMEPLACVFNGLDKIGLLRNPER
                     SAAQYHTLIIGGGPMGTLFASWFRSQHLPYAILENNPWRANFLSSRGYRLADAVSPHT
                     YDGIIVAAGAPELAESALDLVKDGGDLLLFAGFPSDTRLTLNPFSIHYREVSVKGSFG
                     YALAHFRQAYREILGHPALYGELVSHLFPLEDYALAFQKAISGEAMKVVFLVNPL"
     gene            2871..3773
                     /locus_tag="PKM49_06120"
     CDS             2871..3773
                     /locus_tag="PKM49_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82427.1"
                     /translation="MKVRFRGERYYLREIGVAWLCLAPALGLAVVFVFIPLVEVCYLS
                     FTNWDLLRATKSWVGWKNYEYIFKDQKFIQSIWNTLYFAGVKIPLDLVLSLFIAVLLD
                     KRIRGLRFYRASYFAPVVIPVVAASLIWVWLFDPGLGPLNQILGLFGISPSRWLHDPA
                     SAMFSIVLFSLWKGLGYDIVIFLAGLQGIPPTYSEAASIDGAGSWNIFFKITLPLLSP
                     VIFFVILMGIINSFKIFTEISVMTPKGGPLYSTGVIVFYIYQQAFENYRIGRAAAASI
                     VLFFLILAITMVQRKLGRKFVDYE"
     gene            3781..4593
                     /locus_tag="PKM49_06125"
     CDS             3781..4593
                     /locus_tag="PKM49_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82428.1"
                     /translation="MRKLWWVYVLLSIGLIAMLFPFFWMVLSAFKSNADVYVYPPKWF
                     PSQWNFDNFKRVFEMVPFALYFFNSLLVTVLVTYGQLFISVLAAFALSRLKFPFKNAV
                     FMLIVSTMLMPFVVTMIPVFLIVSAFRWIDTYQGLVIPSLFSGFSIIFLRQFFISIPQ
                     DLQDAAMIDGCGFFRILFQVILPNAKPAIATITLFNFLGEWRNYLWPLIVTNSTKMRT
                     LPVGLRYLMTEGGGEYHLMMAASLMAILPVLIVYLFSEKQFIKSVAFTGLKS"
     gene            4607..5863
                     /locus_tag="PKM49_06130"
     CDS             4607..5863
                     /locus_tag="PKM49_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82429.1"
                     /translation="MRKFTLILMVLLVFCTLGLAKTKITFWYAISGVSGEAVKALIDR
                     FQAENPDIVVEAIYSGKYADTAQKITAALAANALPNGGIIPAGPIFTGPRGNYAILDY
                     MLKDPTFDMSDFYEGMWDYSKYKGKICAVPYNISTPVLYYNKELMEKAGLDPTKPPTT
                     WQELLEYSKKITQDTNSDGIPDIWGLNMADTAWIFKSFLLQNENDIVQAVPQKDDYEI
                     IPLFDRPSALETAEYWKQLIDEKAMPMGLHSLAEKQFLGNTLGFYLGTSARIGTWTGN
                     TSFDFGVGYLPKGKRLGIPIGGAVLVLFPAPKDQMDATYQLIKWLTQPDKVAEFSMKT
                     GYIPIRKSSLALPEMKAFFETNPFYKTAFEQSEYAFSYWHFDAMGTMDGLITEVLEKI
                     ERGVSSPKEGMRWLQETTIEEIEASL"
     gene            5867..6661
                     /locus_tag="PKM49_06135"
     CDS             5867..6661
                     /locus_tag="PKM49_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HNY82430.1"
                     /translation="MSIAFSTAGLYPIESIEALKLVSKTGFLEAELMPQCYHEVTESF
                     ALETRKALKSVGAPLRVSSIHYPLVFFATFYNPYTGMRKETRRLSERVASCASILGTE
                     VIVIHAPSKPKPGDVPEYLETVLENIRHLCEVCEELGITVALENNPKTIADTPERWRE
                     AVALIGRPNLKPMLDTTEASEAGILPTAFLDAALCAHYHLSDHSGVDKHLLPGEGDQD
                     WSSFFEKNKNNGYRGIYVAEPSYRLMLTDPIGNLKKVRRFFDQYLP"
     gene            6670..7653
                     /locus_tag="PKM49_06140"
     CDS             6670..7653
                     /locus_tag="PKM49_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNY82431.1"
                     /translation="MRVGLLANPGAGRYALVPEAIRRYQDAFPEHALYAPEGRFFPNE
                     VPGVHTILSRASDSECFLEELSRAVSILVQRCDRLVAFGGDGFFNHICSRLIDESKTL
                     PFTGIGTGTANVGVLTQFDSEELSATLSLETIEPVSPLAIFRDGEPIGYAFHDIVIGD
                     TFLGTVEGSTRNLSAKKWVVDRVLQPVKPKSLSIGEEFRIFCGERPLPMKSATVAQII
                     VSPIFKGKGFAGKAVSGLLCFSPYYADCFCIALVDRILVEMTTDFPTDGCVIEHYLFR
                     EGEALRMEGLSPDHYVIADGNPLCPANGSLRITRSERRVSVLMNPHRFQCF"
     gene            7679..>8560
                     /locus_tag="PKM49_06145"
     CDS             7679..>8560
                     /locus_tag="PKM49_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNY82432.1"
                     /translation="MTNVKFTQALEAFAVGDAVGLPTEFMTREEIVRVYPVIDGLPNP
                     ELSRHHSDLPAGSVSDDTEQNLYLIDAFREKGVSTEATVYALLKWIEESRAVEKRFIG
                     PSSLKSLRAIQNGGSPQKAGYGNTTCGAVMRVLSVSLCTPFGCWDQLATHIYLTTLPT
                     HFSESALEAAMSFGFALAAAAGKASLEGVIAAFFGGAARAKAFGAPEYSIPSTVSRVR
                     FFIEHQDRFEDREQVADYLSSVQGSGLYANEVAPAAFILFLTSPHDCWGNIRLAASLG
                     GDTDTIAALAGALTALHN"
BASE COUNT         1848 a         2305 c         2339 g         2068 t
ORIGIN      
        1 tgaaagttgg cgttcccaag gcgttcccaa gcgtatcgcg ccgtcgtaaa aagaagaagc
       61 gcttacgccg cggtgagaac gaggccccca caggttgaca gtcagagaaa aaaggggtat
      121 aatggtagtg aactaatgta tgcgcacata ttctctttct ggagggatcc gatgaccgga
      181 ctggagaaga acctccgcag gctctttcgc cctaatggga agaccctgtg ggttgcttta
      241 gaccatcccc aattctgtgg ccctatgccg ggtctggaaa acccgattca gacgatcgga
      301 agaattctac tcgctccaca cgacgggatg atccttaacc ccggcatctt ccgcttgttg
      361 cctacggaag cctatcaaaa acgtctcgtc atgcgcgtct ctgtgggagg cacctgtttc
      421 tctgattatt ctaaggtcca tccggtcgtg gtggatccgg ttcaggcctt gtgcgaaggc
      481 gccgacgccg tgataatgat gttgattttg gggagtcaag acgcgttggc gatggagtcg
      541 gttcgtcgac acatcgaagc ctatcaccga tacgccatac cggtggttgt cgaggtgatg
      601 tcggaggact acaccctgaa caacgacttc cagcacgtgt atgcgggcgc gaggatagcg
      661 gcggaattgg ggtgcgacat cgttaaagtc ttctactgcg aagggttcca ccgtgtcgtg
      721 gagacctgcc ccgtaccggt tttattggcg ggcggtccga aggacgcgga tgtactcgaa
      781 ggcgcgaaag aggccctccg ttccggcgcg aaaggcttcg ctttcgggcg caacgtcttc
      841 caaaacgaaa atcccgtcga gatgatcgac cggctttcgc ggctattgga ggggtgacca
      901 tacccaagcg tatcccgaac cacctcctct acacgatcgc cgtcgattat tacctgagcg
      961 gcgccactca ggaggagatt ggcgagaagt acggcatctc gaggccgcag atttctcggt
     1021 acctacgaaa agccagagag gagaaaatcg ttgaaatcac cgttcatcct ccgactgaga
     1081 tcgatcaagt ggctttggag agaggtttgg aacaaaaata cggtttgaaa aaggctgtcg
     1141 tcgtcccttt tctccggcgg aagtatcctc cggaggagtt gatccgaacg atcgcgatca
     1201 gcgcagcgaa aactgtcaag aaactgatct cgatccatca gcgaattggc atcggttggg
     1261 gaaagaccgt ttaccagacc gttctggcga tatgctccga tcgctcgggt gaacgaccca
     1321 aacctcttat gaagcgggag ctggccttct atccgctcat tggcggcctg ggccaatcgc
     1381 tgccttacta ccaggtgaac acgatgatcg accgtatggc ggagtattat cacgcgaaga
     1441 gtcggttttt tagcgttcct gcgatgacac taaaagggtc gatgcttcct acggcgatgc
     1501 gtgaagacta cgagtctata gtcgaggttt ggaagtcagt cgatctcgcg attatcggcc
     1561 tcggtggacc gatacaacaa tctgagatac tcaaatcgga gatagacaag gataccatcc
     1621 aacggctgat cgagcagcgg gccatcgggg atatcttggg ccggttcttc gatgaaagag
     1681 gggtgacgtg tccaaccgac ttagactccc gtctactcgg gttggacttg gagcggctaa
     1741 aaagtataaa agacgtggtc tgcgtatgcg gagggaagaa taaggtccgg gctatacaag
     1801 tcgccctaga acacggtttt tttaatcacc tgatcactga tcagcagacg gcgagcgagc
     1861 tttgaggggg tggatctatt gaaagccttg atttattcag ggccagggca gctgaacatc
     1921 caagagattc cggcgccaca agggaaaacg atcgtcgaag tcggctacgc cggtatctgt
     1981 gggaccgatg tcaagacctt tttgaagggg cacccgatgt ttaaaccgcc tacggtcttg
     2041 ggtcacgaat gtatcggccg tatcgcgcga accgacgctc ccgagaggga tttagccgag
     2101 ggaatgctgg tcgcggtggc tccctacgcc gagtgtggcg tttgcgagaa ctgtttgaaa
     2161 gggtatcccg agctctgccg acaaaagtcc ttcgcttcgt ccgggagttt ctgcgaactt
     2221 ctgggaatgg ataccgagta cgcgaaccgg gcgctcttcg tgatacctga cgcgcagccg
     2281 gtctatacgc tgatggaacc gctcgcttgc gttttcaacg ggttagacaa gataggtctt
     2341 ctcagaaacc cagaaaggtc tgcagcccag taccacacgc tcatcatcgg aggcgggccg
     2401 atggggaccc tattcgcctc ctggtttcga tctcaacacc tcccgtacgc gatcttggag
     2461 aataacccct ggcgcgcgaa cttcctctcc tcgagagggt accggttagc ggatgcggtc
     2521 tcgccccaca cttatgacgg tatcatcgtc gcggctggcg ctcccgagct cgccgaatcc
     2581 gcgctggatc tggtgaaaga cggaggagac ctcctacttt tcgcggggtt tccctccgac
     2641 acccggttaa cgttgaatcc tttctcgatc cactacaggg aagtgtctgt gaaagggagc
     2701 tttggctacg cgctcgccca cttccgtcaa gcctatcggg agatcctcgg tcacccggcc
     2761 ctttacggag aactggtctc gcacctcttc cctctggagg actatgccct agcctttcag
     2821 aaagcgatct cgggagaagc gatgaaagtg gtctttctgg tgaacccgtt atgaaagtgc
     2881 gttttcgagg ggaacggtat tatctccgtg aaataggcgt tgcgtggctc tgtttggcgc
     2941 cggctttggg attggccgtc gtttttgttt tcattccgct cgtggaggtc tgctacctca
     3001 gcttcaccaa ctgggacctg ttacgcgcga cgaaaagttg ggtcggttgg aaaaattacg
     3061 agtacatctt caaggatcaa aagttcatac agtcgatctg gaacacgctt tacttcgccg
     3121 gggtcaagat acctttggac ctggtgctgt cactcttcat cgccgttttg ctcgataaac
     3181 gcatcagagg gctgcggttc tatcgggcct cctacttcgc gcccgtcgtg attccggttg
     3241 tagccgcctc actgatctgg gtgtggttgt tcgaccctgg tctcgggccg ctcaatcaaa
     3301 tcctgggatt gtttggcatc agccctagcc gctggcttca cgatcccgct tccgctatgt
     3361 tttcgatcgt tctcttctcc ctttggaagg ggttgggata tgatatcgtg atcttcctcg
     3421 cggggcttca aggcattccg cccacctaca gcgaagcggc cagtatcgat ggcgcgggct
     3481 cgtggaatat ctttttcaag atcaccttgc cgcttttgtc ccccgtgatc ttcttcgtca
     3541 ttttgatggg tatcatcaac tctttcaaga tattcacgga gatttctgtg atgacaccga
     3601 agggcggacc gttgtactcc acgggagtga tcgtcttcta tatctatcag caggcctttg
     3661 aaaattaccg tatcgggagg gcggcggccg cttcgatcgt gttgttcttt ttgatcctcg
     3721 cgattacgat ggtccagaga aagctcggca gaaagttcgt cgactacgag taggtggtct
     3781 atgagaaaac tatggtgggt ttacgtcctc ctttcgatcg gtctcatcgc gatgctgttc
     3841 ccgttttttt ggatggtttt gtccgcattc aagtcaaacg ccgacgtcta tgtctatccg
     3901 ccgaaatggt tcccctcgca atggaatttc gacaatttca aacgggtttt cgaaatggtc
     3961 cccttcgcgc tatacttttt caacagcctt ctggtgaccg ttctggtgac ctatgggcag
     4021 ctctttatct ccgttttagc cgcgtttgcg ctttctaggc tgaaatttcc gttcaaaaat
     4081 gcggtcttta tgctgatcgt gtcgacgatg ctgatgccgt ttgttgtgac gatgattccg
     4141 gtattcttga tcgttagcgc cttcaggtgg atcgacactt accaagggtt ggtgatcccg
     4201 tcgcttttca gcgggttttc gatcatcttc cttaggcaat tcttcatatc aatcccgcaa
     4261 gacttgcagg acgcggcgat gatcgacggg tgcggttttt tccgcatcct gttccaagtc
     4321 attctcccga atgcgaaacc ggcgatcgcg acgatcaccc tattcaactt tctcggagaa
     4381 tggcgtaact acctgtggcc gctcatcgtc acgaactcta cgaagatgcg gacgctgccg
     4441 gtagggttgc gctatttgat gaccgaaggg ggcggcgagt accatttgat gatggctgcg
     4501 tccttaatgg ccatcctccc cgtgttgatc gtctatctct tctcagaaaa gcaatttatc
     4561 aaatccgtgg cctttacggg tttgaaatca taaaggaggg ttttctatga ggaaatttac
     4621 gcttatctta atggtccttc tcgtattctg caccttaggc ctagcgaaaa cgaagatcac
     4681 attttggtac gccatcagtg gggtgagcgg cgaagcggtt aaagccctga tcgaccgctt
     4741 tcaagccgaa aacccagaca ttgtcgtgga agctatatac tccggcaagt acgcggatac
     4801 ggcccaaaaa ataaccgccg ctctggccgc caacgcgctt ccgaacggtg ggatcatccc
     4861 ggcgggcccg atttttacgg ggccgagagg taactacgcc attctcgatt atatgttgaa
     4921 agacccaacc ttcgatatgt ccgattttta tgaaggtatg tgggactact ccaagtacaa
     4981 gggtaaaata tgcgcggttc cctacaacat cagcaccccc gtgctctact acaataaaga
     5041 gctgatggaa aaagccggtc tcgaccccac gaaaccgccg accacgtggc aagaactcct
     5101 cgaatactcc aaaaaaatca cccaagacac gaactcggac gggatccccg acatctgggg
     5161 gctcaatatg gccgataccg cgtggatctt caagtccttc ttactccaga acgaaaacga
     5221 tatcgtacaa gcggtcccac agaaggacga ctacgaaatc attccgctct ttgatcgccc
     5281 gtccgctttg gaaactgcgg agtattggaa acagttgatc gatgagaagg cgatgcccat
     5341 gggtctccac tccttggcag aaaaacagtt cctagggaac acgcttgggt tctatctcgg
     5401 tacttccgcc cgcattggaa cctggactgg gaacacgtcc ttcgatttcg gagtcggcta
     5461 tctcccgaaa ggcaagcgtt tggggatccc cataggcggt gccgttctcg ttctcttccc
     5521 cgctcctaaa gaccagatgg acgccaccta tcaactgatc aagtggctga cacagccgga
     5581 caaggtcgct gaattctcga tgaaaaccgg gtacatcccg atccgtaaat cttccctggc
     5641 attgccggag atgaaggcct tcttcgagac gaatccgttc tacaaaaccg ccttcgaaca
     5701 gtctgagtat gccttctctt actggcactt tgacgcgatg gggacaatgg acggcttgat
     5761 cactgaggta ttggagaaga tcgaacgggg agtttcttct cctaaagaag ggatgaggtg
     5821 gctccaagag acgactatcg aagagatcga ggcgagtctg tagcttatgt ctatcgcgtt
     5881 ctcgacggcc ggactgtacc cgatcgaatc gatcgaagct ttgaagctgg tttctaaaac
     5941 cggttttcta gaagcggaac tgatgcccca atgctatcac gaggtcaccg aatccttcgc
     6001 cctagaaacg agaaaggcgc tgaagagcgt gggcgctcct ttgcgtgttt cttcgatcca
     6061 ctacccgctg gtctttttcg ccaccttcta caatccctac accgggatgc gaaaagagac
     6121 ccggcgcctt tccgaaaggg tggcctcttg cgcatcaatc ctcggaacgg aggtcatcgt
     6181 tatccacgcg ccgagcaaac cgaaaccggg cgatgttccc gagtatctcg agaccgtgct
     6241 cgaaaacatc cgacatctct gcgaggtttg cgaagagctg gggatcacgg ttgcgctgga
     6301 aaacaatccc aaaacgatcg ccgacacccc ggagcgctgg cgtgaagccg ttgccctcat
     6361 cggtcgcccc aacctgaagc cgatgctcga cacgaccgag gcttcggagg ctggtattct
     6421 accgactgcg tttttggacg ccgcgctgtg cgcgcattat catctgagcg atcattcggg
     6481 ggttgacaaa cacctgttac cgggtgaagg agaccaggat tggtcctcgt tctttgaaaa
     6541 gaacaagaat aacggctatc ggggaatcta tgtggcggaa ccgtcctacc gattgatgct
     6601 cacagacccc atcggcaacc tcaagaaggt cagacggttc ttcgaccagt atctgccgtg
     6661 aggacgcctt tgagggtcgg gcttttagcc aatccaggcg cgggccgata cgcgctcgtg
     6721 cccgaagcga ttcggcgata ccaggacgcc tttccggagc acgcgctata cgctccggaa
     6781 gggcgttttt tcccaaacga agtccccggg gtccatacga ttctgtctcg tgcatccgat
     6841 tcagagtgct ttttggaaga gctgtctcgc gccgtttcga ttctcgttca aaggtgtgac
     6901 cgactcgtcg cctttggagg ggacggtttc ttcaaccaca tttgctcgag actcattgac
     6961 gaatcgaaga ctcttccgtt cacgggtatc ggcaccggaa cggccaatgt tggcgttctc
     7021 acgcagttcg attcggaaga actctccgcc acgttatcct tggagacgat cgagcccgtc
     7081 tcccctttgg ccatattcag agatggcgag ccgatagggt acgccttcca cgatatcgtc
     7141 atcggagaca cgttcttggg taccgtcgag ggttcgacgc gcaacctcag cgccaaaaaa
     7201 tgggtagtcg atcgggtttt gcagcccgtg aagcccaaat cgctttctat cggagaggaa
     7261 tttcggatct tctgtgggga gcggccgctt ccgatgaaat ctgccactgt cgctcaaatc
     7321 atcgtttctc cgatttttaa agggaaaggg ttcgccggta aggcggtttc gggattgtta
     7381 tgcttcagcc cgtattacgc ggactgcttc tgtatagctc tggtcgaccg cattttggtg
     7441 gagatgacga ccgattttcc gaccgatggt tgcgtgatcg aacactacct tttccgagag
     7501 ggagaagcgc tgaggatgga agggctttca cccgaccact acgtcatcgc ggatggtaat
     7561 cccttgtgcc cggcaaacgg gtccctacgg atcacacggt cggaaagacg tgtgtccgtc
     7621 cttatgaatc cgcaccggtt tcaatgcttc tagtttgaca agtaatgggg gtcagattat
     7681 gactaacgtt aagttcactc aagcactcga agccttcgcc gtcggggacg ccgtcggact
     7741 tcccacggaa ttcatgacgc gagaagagat cgttcgcgtg tatcccgtga tcgatgggct
     7801 tccgaatccc gaactctctc gccaccattc cgacctgcct gccggaagcg tttcggacga
     7861 caccgagcaa aacctctacc tgatagacgc gttccgagaa aagggcgttt ccaccgaagc
     7921 gaccgtctac gccctattga aatggatcga ggaatcgcgg gcggtggaaa agcggttcat
     7981 cggcccgagc tcgctgaagt ctttacgagc cattcagaat gggggatctc cgcaaaaggc
     8041 gggctatgga aacacgacct gcggggccgt gatgcgtgtc ctatcggttt ctttgtgcac
     8101 tcccttcgga tgttgggacc agttggccac tcacatctat ctgaccacgc tgccgacgca
     8161 tttttccgaa tcagcgttgg aggccgctat gagttttgga ttcgctttgg ccgcagcggc
     8221 gggcaaggct tctctggaag gggtcatcgc cgcctttttc gggggcgctg ccagggccaa
     8281 ggccttcggc gcaccggagt actcgattcc ctctacggtg tctcgtgtcc ggttttttat
     8341 agaacatcaa gatcgattcg aagatcgaga gcaggtggcc gactaccttt cttctgtgca
     8401 ggggagcggg ctttacgcta acgaagtcgc ccctgccgct ttcatcctct tcctcacttc
     8461 cccccacgat tgttggggca atatccggtt agccgcgtct ttgggtggcg acaccgatac
     8521 gatcgccgcg ctcgccggcg ccctaaccgc cctacacaat
//
LOCUS       DAOPXN010000089         8536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8286_length_8536_cov_4.781394, whole genome shotgun sequence.
ACCESSION   DAOPXN010000089 DAOPXN010000000
VERSION     DAOPXN010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8286_length_8536_cov_4.781394"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PKM49_06150"
     CDS             <1..501
                     /locus_tag="PKM49_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNY82433.1"
                     /translation="ILILSKRDFHSISTEIEWETIFFFLGLFAVTRALKDVGITGAVA
                     TAISRMQVPPVFVVLTVLWVSGLLGGIIGAVPITTVFIPIVQSLQGTIPHSYELWWAL
                     ALGTGLGGSTTFSGAAANMVAVGLIEKNYKGKVKYLAFFKEGLSVTLIGLTVATAYLA
                     IRWFML"
     gene            539..1426
                     /locus_tag="PKM49_06155"
     CDS             539..1426
                     /locus_tag="PKM49_06155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82434.1"
                     /translation="MREISFQSMINTLDLFSTGVAIVDQEGRFLFWNSGASLITGHTA
                     QKIVGKTFDEAMTMIDVFGQKISFEYFLEMHCLRGNAASLEVWIETPEGLLTHMRLYS
                     ARLTDGEGTCLGFAMILHDVIIEEVLGDALEKVAEQAHIDALTKLYNKETLLEDLRTH
                     IENVKRAKYQIGIVFMDIDRFKSINDRFGHITGDSVLSLFGKCILSSIRKGERAYRFG
                     GDEFVVLLIIDSEEDLLRFTQRFEREVYETKFPVTLNVTIGFTVVLGTDSARSALERA
                     DQRMYEKKIGKTHTETKTP"
     gene            1423..2448
                     /locus_tag="PKM49_06160"
     CDS             1423..2448
                     /locus_tag="PKM49_06160"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNY82435.1"
                     /translation="MISEKKAAEVTAFLERYRSFLIVSHIQPDGDAISSVLSLSFGLR
                     RLGKETQAAIDWPIPEVFQSIPLCDQIRGYPIDNGFEALIFLDGSSPDRMGRFAKWLD
                     EKPSCVIDHHRSALSFGTVQWVEPTASATAQLILMLNRRLGVPYDPVLATVNMLGLQT
                     DSGFFRYSNTTADLFKDAAFLMEQGAQPYFNASTILENKKTEEFHLLTRMIQKLVILL
                     SGKLVYSYITQEMLRECECTEEDTAGFIGELRSIKGTECAVLFVESEAGDTRVSFRSK
                     KWFDVSQVAAYFGGGGHLKASGCSLRGDFFANIEKVVRYIERQMAQSDASKALLTLLS
                     DRGQNEK"
     gene            2460..3263
                     /gene="minD"
                     /locus_tag="PKM49_06165"
     CDS             2460..3263
                     /gene="minD"
                     /locus_tag="PKM49_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNY82436.1"
                     /translation="MGQVFVITSGKGGVGKTTVTANLGAVFSRLGKKTCLIDADIGLK
                     NLDVVLGLENRILYTIIDVITEKVGSMEALVRHKTSRNLYLLPASQIATKEMMKPEDM
                     EKVVAQLRPNFDIILIDSPAGIESGFRNAVVAADAAIIVTNPEYTAVSDADRVTGLLE
                     KSKISEENIFLIVNRFRPNVAEKRGMLSIDDVTKTLAIPLVGIVPDSEAVVIGSNRGL
                     PIAFESDNELNKIFTNVARRMLGHKVPLQEDYKLSKPTFWRLFKSLFKS"
     gene            3278..4387
                     /locus_tag="PKM49_06170"
     CDS             3278..4387
                     /locus_tag="PKM49_06170"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014736836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNY82437.1"
                     /translation="MDQSEKHHGLLPENEENAVDSVVSTQRVVLTDKHSLFQIQEKIK
                     PAAEALTRGELAVFPTETVYGLGAGIYAAKGVQRIFCVKGRPADNPLIVHIADINQAL
                     DLIHESTPDLFFLYAERFWPGPVTFVVPKSQRVSDKITGGKPSVAIRFPSHPVAQILI
                     NSAGQPIAAPSANISGKPSATRPEHLRELEGKVEWIIDNGPITIGLESTIISLLQTPP
                     VLLRPGPITVEKLKEIIPNLEINLKSSKALAPGMKYRHYAPEAAIAIIPWTGNLTKLA
                     KDTEELYLRLRGEDKEALILATAETINYHSVRGYSAISMGSRSNLYSIAQNLFHSLRT
                     VDALGYRWGIIEGFEEVGLGLALMNRIEKASSGHF"
     gene            4406..5008
                     /locus_tag="PKM49_06175"
     CDS             4406..5008
                     /locus_tag="PKM49_06175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY82438.1"
                     /translation="MTRPIGQWQELGRMLRQRRNYLLLTPEDVFDKTHISVETVDRLE
                     TGSFNEIPAIYLQDFLKRYAALLGLPEQRVFDQYSDGLTDYETKREKERHLSRMKTRV
                     GPLTRWLKYLAGILAIVILTQTLVVVKLLEETQLRIRNDGPGIVTLTSNHNTYALASR
                     ESLRFSSSGPLKISNPDKSVVVIQYGQYEKEVSWTEFEVR"
     gene            5011..5568
                     /locus_tag="PKM49_06180"
     CDS             5011..5568
                     /locus_tag="PKM49_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNY82439.1"
                     /translation="MGRIRQLELVNLVVFAFGAFIDYRLETALPILIERFGETDYTSE
                     LLDFGKYTFYYDREMGNGPIQGRLISFKELVHPYELPSIKIATNEIENALQTDGKRTI
                     NLDSGYIHHTQFVLASTKHWANRLYLGRGIYAEITLMYVDGAFTPLPYTYPNYRAQEY
                     LEPLTKIREGYLEKRKLLYGKSRKG"
     gene            5589..6905
                     /gene="rimO"
                     /locus_tag="PKM49_06185"
     CDS             5589..6905
                     /gene="rimO"
                     /locus_tag="PKM49_06185"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNY82440.1"
                     /translation="MKIYIETLGCPKNEADSQMLKRLLASGGQIVSTPVDAEVVVVNT
                     CAFIQDAQKESIETLLEYLLRWKQEQPGRRVYAWGCLVQRFIKALQKEIPELDGLIGV
                     LPVERISEGLFSHWREKTDQPLVLHAEDPDTSCALSSQYFESPPFYAYVKIGDGCDRG
                     CGFCTIPFIKGPHRSRPITHICQEVKELVGKGKKELILVDQDTTQYRDGERRLPDLLR
                     ALNAIEGDFWVRVLYIHPDHFDLPLLESFAACDKVLPYFDIPIQHASDSVLQAMNRIK
                     SQKELRTIFKLIRERIPEAVLRTTVMVGYPGETRQDFDRLVAFLKEIQFDRLGVFRFS
                     SERGAPVNRKRLQRVSAKEGASRLDEIMTLQQELSYEKNRRFLGKVVRVLVETAPSHY
                     VGRTIRDAPEIDGSFDIYTDETLTLGEFYTCRVTETDVYDLGGTPA"
     gene            6902..7441
                     /gene="pgsA"
                     /locus_tag="PKM49_06190"
     CDS             6902..7441
                     /gene="pgsA"
                     /locus_tag="PKM49_06190"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNY82441.1"
                     /translation="MTITNATKITLFRVLITPAIMLLIELGGTNRALLWLTFVLYILG
                     ALSDWLDGYVARKHKEVTDLGKFADQLADKAFVTGILCVLTYLQAASYWVVAMIVLRD
                     TFVNGLRMIYASKGTIVAANWWGKLKTVIQLVFIGILLAQRITPLFPDSALVLFSWIT
                     VAVTVVSGLTYLPKKGGKT"
     gene            7438..7986
                     /gene="thpR"
                     /locus_tag="PKM49_06195"
     CDS             7438..7986
                     /gene="thpR"
                     /locus_tag="PKM49_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNY82442.1"
                     /translation="MRAFLALKISEEIESEALKASLELQQRQFEGSWVKSGNFHLTLL
                     FLGERTPDEIQRLSGLLEKNLQFAPFALETDRLGFFKKGGKPTVCWLGIKKHGAFSAL
                     ADELTRLLLPLQTDPIDSESAHITLGRLKKVPDQWHAFIRQIEIPSMIFTVRHVFLFK
                     SVLTPQGPVYTCLGKYNKNEGA"
     gene            8004..>8536
                     /locus_tag="PKM49_06200"
     CDS             8004..>8536
                     /locus_tag="PKM49_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HNY82443.1"
                     /translation="MEITNKDKKKALDSAIHQIEKDFGKGSIMIMGSDSLNANIETIP
                     TGSISLDIALGVGGYPRGRIIEIYGPESSGKTTITLHAIAQAQKKGGVAAFIDAEHAL
                     DPIYAKNLGVDLESLLISQPDCGEEALEIVETLVRSGAVDIIVIDSVAALVPKAEIEG
                     NMGDMQMGLQARLMSQDL"
BASE COUNT         2100 a         2162 c         2315 g         1959 t
ORIGIN      
        1 atccttattc tcagtaagcg cgattttcat tctatctcta cggagatcga atgggaaacg
       61 attttctttt tcctcgggct ttttgctgta acgcgagccc tcaaggacgt gggtatcacc
      121 ggggccgtgg cgacggccat ctcgcggatg caggtccccc cagttttcgt cgtcttgacg
      181 gtcttgtggg tctcagggtt actcggaggt attataggtg cggtccccat caccacggtt
      241 tttatcccga tcgtgcaatc gcttcaaggg acgattccgc acagttatga actgtggtgg
      301 gctttggcct tgggcacagg tttgggaggg agcaccacct ttagcggcgc ggccgcgaat
      361 atggttgcgg tcggcttgat cgaaaaaaac tacaaaggaa aggtaaagta tttggccttc
      421 ttcaaagaag ggctcagcgt gaccttgatc ggtttgaccg tggctacggc ttacttggcg
      481 atccgctggt ttatgctata atctcatata gaagcgcaaa cgaaaggaac gtgatgagat
      541 gcgcgaaata tccttccaat ctatgatcaa cacgctcgac ctcttttcca ccggcgttgc
      601 gatcgtcgat caagaagggc gttttttgtt ttggaatagc ggcgcctctt tgataacggg
      661 ccataccgcg cagaagatcg tcggtaaaac cttcgatgag gctatgacga tgattgacgt
      721 cttcggtcaa aaaatctctt tcgagtactt cttagagatg cactgtctac gtggaaatgc
      781 ggcctcgctc gaggtgtgga tcgagactcc ggaaggctta ttaacccata tgagactgta
      841 tagcgcccgc ttgacggacg gtgaaggaac ctgtttgggt ttcgccatga tcttacacga
      901 cgtgattatc gaggaagtgc tgggcgacgc cctcgaaaag gtcgctgaac aggctcatat
      961 tgatgcgctg acgaagttgt acaataaaga aacccttctg gaggacctac gcacccatat
     1021 cgaaaacgtc aaacgggcga aataccagat tgggatcgtt tttatggata tcgaccggtt
     1081 taaaagcatc aacgaccggt tcgggcacat caccggcgat tcggtgctct cgctcttcgg
     1141 caagtgcatc cttagcagca tccggaaagg ggaacgggct tatcggttcg gaggagacga
     1201 attcgtcgtg ttgttaatca tcgattccga ggaggacttg ctccgtttca cgcagcgctt
     1261 cgaacgcgag gtgtacgaga cgaaatttcc ggtgaccttg aacgtgacca tcggtttcac
     1321 ggtagttctc gggacggact ccgctcgatc cgcgttggaa agggcggatc aaagaatgta
     1381 cgaaaaaaag ataggaaaaa ctcatacgga gacaaaaacg ccgtgatttc tgaaaaaaaa
     1441 gccgcagagg tgacggcgtt tttggaaagg taccgctctt tcttgatcgt gagtcacata
     1501 cagccggatg gagacgcgat tagctccgta ctttccttga gttttggttt acgtcgtctg
     1561 ggtaaagaaa cccaagcggc gatcgattgg cccatcccag aggtctttca gtcgattcct
     1621 ttgtgcgacc aaatccgtgg gtatccgata gacaacgggt tcgaggcgct aattttcctc
     1681 gacgggtctt cacccgatcg tatgggtcgg ttcgctaaat ggttggatga aaagccctcc
     1741 tgcgtcatcg atcaccaccg ttcagcgctt tccttcggaa cggtgcagtg ggtagagccc
     1801 accgcctccg cgacggccca gctgatcttg atgttgaatc gacgtctggg cgtgccctac
     1861 gacccggtcc tggcgaccgt gaatatgtta gggttgcaga cggattccgg atttttccgt
     1921 tacagcaaca cgacggccga tttgttcaaa gatgcggcct ttttaatgga acagggcgcc
     1981 caaccttact tcaacgcctc caccatttta gagaacaaaa aaacggagga gttccacctc
     2041 ttgacccgga tgatccaaaa actcgtaatt ttactctccg gtaaactggt ctattcctat
     2101 atcacgcaag agatgctccg ggagtgcgaa tgcacggaag aggataccgc cgggttcatc
     2161 ggtgagttgc gatccatcaa ggggaccgaa tgcgcggtcc tgttcgtcga atccgaggcc
     2221 ggggacaccc gcgtgagttt ccgctcgaag aaatggttcg atgtctctca agtcgccgcc
     2281 tatttcggag gcggcggcca tctgaaggct tccgggtgtt ctttacgggg cgacttcttc
     2341 gcgaatatcg agaaggtcgt ccgatatatc gagcgccaaa tggctcaaag cgacgcgtca
     2401 aaggccttgt tgactctttt gtccgatcgc ggacagaatg aaaagtgagg gggttgaagg
     2461 tgggacaagt tttcgtgatc acatccggaa aaggcggtgt cggtaaaaca acggtaacgg
     2521 ccaacctcgg agcggttttt tctcgtctgg gaaagaagac gtgtttgatc gatgcggaca
     2581 tagggttgaa aaacctcgac gtggtgctcg gcctcgaaaa cagaatcttg tacacgatca
     2641 tagacgtaat caccgagaag gtcggctcta tggaagcttt ggttcgtcat aaaaccagcc
     2701 gaaacctcta tcttctgccg gcttctcaga tcgcaacgaa agaaatgatg aagcccgaag
     2761 atatggagaa ggtcgtcgcc cagttgcgac cgaacttcga catcatcctt atcgattctc
     2821 ccgcgggaat cgagagcggg tttcgaaacg cagtcgtcgc ggcggacgct gccatcatcg
     2881 tgaccaatcc cgagtatacg gcggtgagcg acgccgaccg tgtgaccggg cttctggaaa
     2941 agagcaaaat atctgaagag aacatcttcc tgatcgtgaa ccgatttcgg cccaatgtgg
     3001 ccgaaaagcg cggtatgcta tcgatcgacg atgtgactaa aactttggcg atcccgctgg
     3061 tagggatcgt gccggattct gaagccgtgg tcattggatc gaatcgcggg ctcccgatcg
     3121 ccttcgagag cgacaacgag ctgaataaaa tattcacgaa cgtcgctcga cggatgttag
     3181 gccacaaagt ccccttgcag gaggattaca aactctcgaa accgactttc tggaggttgt
     3241 ttaagtcttt atttaaaagt taaatcaggt gtggagcatg gatcaaagcg agaaacacca
     3301 cgggcttctc ccggaaaacg aagagaatgc ggtcgattcg gtggtttcca cccagcgcgt
     3361 tgttctcacc gacaagcatt ctctttttca aatacaagag aagatcaaac cggccgcaga
     3421 ggctttaacg aggggtgagt tggccgtttt tcccaccgaa accgtttacg gactcggcgc
     3481 cggtatttac gccgcgaaag gcgttcaacg gatcttctgt gtgaagggcc gccccgcgga
     3541 caatcccttg atcgtccata tcgccgatat caatcaagcg ttggacctga tacacgaatc
     3601 cactccggat ctttttttcc tatacgcgga acgattttgg cctgggcctg ttacttttgt
     3661 tgtgccgaaa tctcagcgcg tctcggacaa gatcaccggc gggaagcctt cggtggccat
     3721 tcgattccct tcgcatcccg tagcgcagat attgatcaac tcggctggcc aaccgatcgc
     3781 ggcgcccagc gcgaatatct ccggcaaacc cagcgccact cggcctgaac acttgcgaga
     3841 attggagggg aaggtcgaat ggatcatcga taacgggccg atcacgatcg gattggaatc
     3901 cacgatcatc tccctgctcc aaacgccgcc cgtattgtta aggcccggac cgatcaccgt
     3961 ggaaaagttg aaggagatca tccccaacct cgaaatcaac ctgaaaagtt cgaaggccct
     4021 cgcgccgggt atgaaatacc ggcactatgc gccggaagcc gccatcgcga tcatcccctg
     4081 gaccggaaac ctgaccaaac tcgccaaaga cacggaagag ctctatcttc gcttacgcgg
     4141 tgaagataaa gaggccctga ttttggcaac ggccgagacg atcaactacc actccgtcag
     4201 agggtattcg gctatctcga tgggaagccg gtcaaacctc tacagtatcg ctcaaaacct
     4261 ctttcattcc ttgcgtacgg tggacgcgct cggttatcga tgggggatta tcgaaggatt
     4321 cgaagaagtc ggtttgggtt tggccctgat gaaccggata gaaaaagcct cctcggggca
     4381 cttctaaggg acggaggctt ccgaaatgac gcgtccaatc ggccaatggc aggagttagg
     4441 gcggatgtta cggcaacgcc ggaactacct cctcttgacc ccggaggacg tctttgataa
     4501 aacgcatatc tccgtcgaaa ccgtcgaccg gttggagacg gggtctttca acgagatacc
     4561 ggcgatctat ctgcaagact tccttaaaag atacgccgct ttgctcggtt taccggagca
     4621 gcgcgtcttc gatcaatact cggacgggtt aaccgactat gaaacgaaac gcgagaaaga
     4681 gcggcacctg tctcgtatga aaacgcgggt tggtcctctc acccgctggc tcaaatactt
     4741 ggccggtatt ttggcgatcg tcatcctcac gcaaaccctc gtggtcgtaa agcttttaga
     4801 agaaacccag ctccgtatac gcaacgatgg tcccgggatc gtgaccctca cgagtaatca
     4861 caacacctac gcgctcgcga gcagagagag cctccggttt tcctcgagtg gcccgctgaa
     4921 gatatcgaat cccgataaaa gcgtagttgt gatacaatat ggccagtacg agaaagaagt
     4981 aagctggaca gaatttgagg tgcgttaaac ttgggccgta ttcgtcaact cgagctggtt
     5041 aacctggtgg tttttgcgtt cggcgcgttc atcgactatc ggttggaaac ggctctaccg
     5101 atcttgatcg agcgatttgg agagacggac tacacatccg aactgttgga tttcggaaaa
     5161 tacaccttct actatgaccg cgagatgggg aacggcccga ttcaaggcag attgatcagt
     5221 ttcaaggaat tggtgcatcc gtacgaacta ccgtcgatca aaatcgcgac gaatgagata
     5281 gagaacgctt tgcaaaccga cggaaagcgc acgataaact tggattcagg ctatatccat
     5341 catacccaat tcgttctggc atcgaccaaa cattgggcca atcgtcttta tctgggaaga
     5401 ggcatatatg ccgaaattac cttaatgtac gtggacggcg cgtttacacc tttgccctac
     5461 acctatccca attatcgcgc gcaagagtac ctcgagccgt tgaccaagat acgggagggg
     5521 tacctagaaa aacgcaagtt gctttatggg aagagtagaa aaggataact cgatgaaggg
     5581 aattttgtat gaaaatatat atcgaaacct tgggctgtcc caagaacgaa gcggacagcc
     5641 agatgttgaa gcgcctcctc gcttccgggg gacagatcgt ttcgaccccg gtagacgcgg
     5701 aagtcgttgt cgttaatacc tgcgccttca tccaagacgc gcaaaaagaa tcgatcgaaa
     5761 cgctactgga atacctcttg cggtggaagc aggaacagcc cggaaggcgg gtttacgcgt
     5821 ggggctgttt ggttcagcgg tttataaaag ctttgcaaaa agagatccca gaactggacg
     5881 ggctcatcgg tgtcttgcct gtggagcgaa tttcagaggg tttgttctcg cattggcgag
     5941 agaaaaccga tcaacccctc gtcttgcacg ccgaggaccc cgacacctct tgcgcgctct
     6001 cttcccaata cttcgaaagc cctcccttct acgcgtatgt caagatagga gacgggtgcg
     6061 acagaggttg cgggttttgc accattccgt ttattaaagg tccccaccgg agtcgaccga
     6121 tcacccatat ttgccaagaa gtgaaagagc tcgtgggtaa agggaagaag gaattgatcc
     6181 tggtcgatca ggatacgacc cagtaccggg atggcgagcg gaggctgccc gatctgttac
     6241 gtgcgctcaa cgccatcgag ggcgattttt gggttcgtgt cctctatatc caccccgatc
     6301 atttcgatct gcctttgtta gaaagcttcg ccgcgtgcga caaggtgctc ccctacttcg
     6361 acatccccat acagcacgct tccgattcgg tgctgcaagc gatgaaccgg atcaagtcac
     6421 aaaaggaact ccgcacgatc ttcaaactga tccgcgaacg catcccagaa gcggtcctcc
     6481 gcactaccgt aatggtcggt tacccaggcg agacccggca agatttcgac cgactggtcg
     6541 cttttctgaa agaaatccag tttgaccggc tcggtgtctt ccgtttctct tcagaacggg
     6601 gcgctccggt gaacaggaaa agactgcaaa gggtctccgc aaaggaaggg gcgagccgcc
     6661 tcgacgaaat tatgacctta cagcaagagc tctcttacga aaagaaccgc cgctttctcg
     6721 gaaaggtcgt tcgcgtgttg gttgaaacgg cgccctccca ttacgtgggg cgaacgatac
     6781 gcgatgcccc agagatcgac ggatcgttcg atatctatac cgatgagaca ttgacactgg
     6841 gcgagtttta tacctgtcgg gtcacggaaa ccgacgttta cgatttggga gggacaccgg
     6901 catgacgatt acgaacgcca cgaaaatcac cctattcaga gttctcatca ctccggccat
     6961 catgctcttg atcgagctcg gaggaaccaa ccgcgcgctc ctgtggttaa cgttcgtcct
     7021 ctacatcttg ggcgcgcttt ccgattggtt ggacggttac gttgcgcgaa agcacaaaga
     7081 agtgacggat ttaggcaagt tcgccgatca attggccgac aaagccttcg ttacaggtat
     7141 cctatgcgtg ttaacctacc tgcaggccgc gagctattgg gtcgtggcga tgatcgtgct
     7201 acgagacacc ttcgtcaacg gattacggat gatctacgcc tcgaagggca caatcgtcgc
     7261 ggcgaattgg tggggcaagt tgaaaaccgt catacagctc gtcttcatcg ggatattgct
     7321 cgcgcaacgg atcacccccc tcttcccgga ctcggccctc gtcctcttca gctggatcac
     7381 ggtcgccgtt acggtggttt ccggtttgac gtacttgccg aaaaagggag ggaaaacatg
     7441 agagccttct tagctttaaa aatcagcgaa gagatcgaat ccgaagcgtt aaaagcgagt
     7501 ctcgaacttc aacagaggca atttgaggga tcttgggtaa aaagcgggaa ttttcatctc
     7561 acgctgctct ttttgggaga aagaacgcct gacgaaatcc aaaggctgag tgggctattg
     7621 gaaaaaaact tacaatttgc ccctttcgct ttggaaacgg atcgactggg cttctttaaa
     7681 aagggaggga agcccaccgt gtgttggttg gggatcaaga agcacggagc cttttcggca
     7741 ttggccgatg aattgacacg gttgctcttg ccccttcaaa cggatccgat cgactccgag
     7801 agcgcgcata tcacgctcgg gagacttaaa aaggttcctg atcagtggca cgcgtttatt
     7861 cgacaaatag aaattccgtc tatgattttc accgtgcggc acgttttttt attcaagtcc
     7921 gtcttgacgc cgcaagggcc ggtatacacg tgcctcggta aatacaataa aaatgaaggg
     7981 gcttaattaa aaaaggaggc gacatggaaa tcacgaacaa agacaagaag aaagctctgg
     8041 attcggccat acaccaaata gagaaagatt tcgggaaggg cagcattatg attatgggct
     8101 cggactccct aaacgccaat atcgaaacta ttcctaccgg atcgatatcg ttggatatcg
     8161 ctttgggcgt cggcgggtat ccccgcgggc gtatcataga gatatacggt cctgaatcga
     8221 gcggaaagac tacgatcacg ttgcacgcga tcgcgcaggc ccagaagaag gggggggtcg
     8281 cggcctttat cgatgccgaa cacgcccttg accctatcta cgcgaaaaac ttgggcgttg
     8341 acctcgagtc gctcctgatc tcgcaacccg attgtggaga agaggctttg gagatcgttg
     8401 aaactctcgt acggagtgga gcggtggaca tcatcgtcat cgactcggtg gccgcgttgg
     8461 taccaaaagc ggagatagag gggaatatgg gcgatatgca gatgggtctc caagctcgtt
     8521 tgatgtccca ggacct
//
LOCUS       DAOPXN010000090         8428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8426_length_8428_cov_4.536725, whole genome shotgun sequence.
ACCESSION   DAOPXN010000090 DAOPXN010000000
VERSION     DAOPXN010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8428
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8426_length_8428_cov_4.536725"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="PKM49_06205"
     CDS             complement(<1..2029)
                     /locus_tag="PKM49_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY82444.1"
                     /translation="MKYVIESIDHPLSEVLGRLGIAESAPEGKVLSVVLTKAQVVVRI
                     SHNPDSLDAAHFSFMEKAFARFFCLPARIETVLSVTVHSEDERNEGEGAPTLEAESSE
                     IEDPDSASVSVTAEDPSDVETVNERTPSSVTAAHVQLHTHTHLSAMDSILSVEALVER
                     AAKSGQKAVGITDHEVIQAFPEFYERCQAHKIKPIFGMEGNVVDLTPILMNVEKRYPG
                     TEIKFLQAGWETKPFCVIDFETTGLSALSDDIIEIGAVKVLEGKIVDSFQSFVKPNVP
                     IRETITRLTGITEETVQEAPTLAHVLPKLRDFIGDEVIVGHNVNFDYQFYQQALQKTG
                     EKVTHSVTLDTLALARSLLKMPSYTLDKVAKKLALREESGETLAFRHHRAIEDARVTG
                     LILIELLQMAKKEKRFSFEDIQGLQTEIELNRLHGDSFTVFVQNKRGLKNLYRLVSMS
                     HLEYLGKAPLIPKTRLSEHRTGLFLGTGSPSSELSKAYRMGKDRDELIEIARFYDFIE
                     IMPADAYTDLEEGLNATILKEMYARFYEIGREIGLPALFTGNVHYLDPQDHKAWSVLK
                     ISDMAIRRRGQQFPPSLFDDVKLHYRTTEELLSCAEEMLGDAQKAQEVVIHNPAQLAD
                     QIEWIQPITRTLHPPIIEGAEEEIKTLTMNNMRAVYGNEPLEQITERVKREL"
     gene            complement(2026..2568)
                     /gene="ruvC"
                     /locus_tag="PKM49_06210"
     CDS             complement(2026..2568)
                     /gene="ruvC"
                     /locus_tag="PKM49_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNY82445.1"
                     /translation="MNPLRIIGIDPGYGRVGVGLIEVEGNRYSWLFHRLIETPREASF
                     PERLALIYSEIRAICETHEPDEAAIERLFFVKNVTTAMKVSEARGVIELALYQHRVSI
                     FEYTPFEIKQSVTGNGHAEKGQVQRTLGLLLNLKGTLHQDDASDALAAAFCHANSRKV
                     AKGISAYLNPSTPSRPGGPL"
     gene            complement(2565..3563)
                     /locus_tag="PKM49_06215"
     CDS             complement(2565..3563)
                     /locus_tag="PKM49_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNY82446.1"
                     /translation="MSQIVGIRDVPLILAPLAGVTDYAFRELTRTTVPDCVDGFYTEM
                     VSVKGLLNKNRGSFRTLPKLGERAVIQLFGADPADFPKAIEIVLARAPNAVGIDINAA
                     CPVRKVVRNGAGAALAKDLPTLRRILEAAEPPAHAAGRRLTLKIRKGWEANVNYREVI
                     TMAIGCGVDAVALHGRTVEQGYSGTADLTVLDEIPQAECSRVLWSGDIVDPPSLARVA
                     RFPIAGVLIGRASIGNPWIFRELKSAILGKDYRPPDAQERVCAIERHIALMEGEMRRD
                     RICGAFKKFVVGYTKGIPMARPTRNRLLMSTELDELLKGLYAFLYENETAIPVGEG"
     gene            4341..4417
                     /locus_tag="PKM49_06220"
     tRNA            4341..4417
                     /locus_tag="PKM49_06220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4375..4377,aa:Thr,seq:ggt)
     gene            4485..5429
                     /gene="era"
                     /locus_tag="PKM49_06225"
     CDS             4485..5429
                     /gene="era"
                     /locus_tag="PKM49_06225"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNY82447.1"
                     /translation="MNHDLSCLAKGRALIRSGIVTLVGKPNTGKSSLINAIFQQKVVI
                     VSDKPQTTWHHIRCVYTDGTSQIIFSDTPGVNKPIGPLGKYMTKAVRSALEGSDLVLW
                     LFDCSRGVTEAERYVYDKIQSIEPPVFAVLNKIDLAGPTEIDKVLADMAEFGPFAETH
                     QTSATTGVGLARLMEGIKIRLPEGDFLYDPEVLIDKPIRFIVSELIREKIYRYTSKEI
                     PYETGVYIEHLDEKPSSGLSIRAEIFVSRDNHKGILIGKGGEMIRKIRLSAKRDIEIL
                     FDRKVGLELYVRVKERWMHDENLRTKDLDFETIVRKSR"
     gene            5434..7011
                     /locus_tag="PKM49_06230"
     CDS             5434..7011
                     /locus_tag="PKM49_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82448.1"
                     /translation="MEPKTPLSSASLSSLTEIIGPFYFVGGYVRDLLMRRRSRRHHKG
                     AIDIDIAVDSPMETVRERLAKGFFSNREKMTLTFFWKGLTIDLVSLNGLTIDEELTRR
                     DLTINSMGLSCQRVFDILKEEPDWEGSIRPEDVIDPTGGWSDLRAKILRCPREKNLTD
                     DPVRLVRVFRFQGEFGFEIEDLTFQWIHRHAPTVRLVAKERVSRELEKLFSSPYVVKA
                     LRELKKTRLFRELFPLLTGPLDRRLEDRPYFGQSVFEHSLRVIAGCGSDPLLKWAGFF
                     HDVGTRDGFSRVSSTDEETAPRDNYHPEERSVELLLETAKDWGLPKRIVDPVEKIIRF
                     HQVPVTSRSAIKEASMHFREPKAHNRFFRFVQADRCAADPLFLETDEGKAHKEIVKAV
                     RRMMPKISRCERFFSGKTLLALGLRPTPAFKVLIDHLMKKALELDFNLTERTAQELLE
                     STLRFKERYERLFFDNTEYRRKREYYIDGEVIGVLEEDVKLTVRTEVFLYRFEPEAML
                     PEALRGKNPLLFFSLSS"
     gene            7032..>8428
                     /locus_tag="PKM49_06235"
     CDS             7032..>8428
                     /locus_tag="PKM49_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82449.1"
                     /translation="MAQETATVYYGAQSLYDFQKEPEWLERTRERFLKTLVEQPDLLE
                     SEETVDALGTRIYMKLRLLRDSDLKGPVLTAILAENSEESLRLLALEEDRLSRYRPLY
                     PLMDFFVKDFRKALNPFDFQKIVPYFLIGQLEGFEEFFSLTNEEHYHIAKGILNGIQS
                     NRTYPERSLKAMVKLLGEKYLRAYASMVQQLVTARVEADYAKELRLAKSYVDQAGYLY
                     GAQVSQKADQTLHRAYLSLSSYASCKEDTLIAENRVALASQDTYLSMLPLAMELVDRY
                     ERLTINKEGLEKLILSFIQNYGLKSRLYGDEVTAEIRRKWKGFIDERDLSPMVLNAIR
                     SGILDYAAKTPQTVATPAVATPVVVTTKGSVKGSWLEKLRWPTVFIYGVPVLALLLFI
                     AVLPASLKAKGAKALGMKKKALAFYQKASLSKPEVAEYHVQMAILLGLLHREEEAIQE
                     YRIAPKILSMTEDDDL"
BASE COUNT         1839 a         2267 c         2294 g         2028 t
ORIGIN      
        1 ccaactctcg cttgacccgc tccgtaatct gttccagagg ctcgttcccg tagacggcgc
       61 gcatattgtt catcgttaaa gttttgatct cttcctcggc tccctcgatg atcggcggat
      121 ggagtgtccg tgtgatcggc tgtatccatt ctatctggtc cgcgagctgc gccgggttgt
      181 ggatcacgac ttcttgcgct ttctgcgcgt ctcccaacat ctcttcagcg caactcagaa
      241 gctcttccgt tgttcggtag tggagcttaa cgtcgtcaaa gagcgatggg gggaattgct
      301 gtccccttcg gcggatcgcc atatcgctta tcttgagcac gctccacgct ttatgatcct
      361 gcggatccag ataatggacg ttcccggtga aaagcgccgg taacccgatc tcacgcccta
      421 tctcataaaa acgcgcgtac atctctttca ggatcgtcgc gttgagtcct tcctctaaat
      481 cggtgtacgc gtcggcaggc ataatttcta tgaaatcgta gaaccgggct atttctatga
      541 gctcgtcgcg gtcctttccc atacggtagg ccttcgatag ctcgctgctc gggctgcccg
      601 ttcccaaaaa caaccccgtt cgatgctccg agagacgggt tttcggaatg agcggggctt
      661 tacccaaata ctccagatgg ctcatcgaaa cgagacggta taggtttttc agcccgcgct
      721 tattctggac aaagaccgta aaactgtccc cgtgcaggcg gttcaactcg atttcggttt
      781 ggaggccttg aatatcctca aaggagaagc gtttctcttt tttcgccatt tgcagcagtt
      841 cgatcaaaat caagccggtg acacgcgcgt cttctatcgc cctatggtgg cgaaaggcga
      901 gcgtctcgcc ggactcctcg cgcagcgcca gctttttcgc gaccttgtcc aacgtgtacg
      961 acggcatctt caacagagaa cgcgcgaggg ccagcgtgtc cagtgttacg ctgtgtgtga
     1021 ccttctcacc ggttttttgt aacgcctgct ggtagaactg atagtcgaag ttcacattgt
     1081 ggccgacgat cacctcgtct ccgatgaagt ctctgagttt tggcagcacg tgcgcgagcg
     1141 ttggcgcttc ttgaacggtc tcttcggtga tgccggtcag tcgcgtgatc gtctccctga
     1201 tcggcacgtt tggcttcacg aaagattgga aggaatcgac gatctttcct tctaagacct
     1261 tgacagctcc tatttcgata atatcgtcgc taagggcgct cagaccggtc gtttcgaagt
     1321 cgatgacaca aaagggcttc gtctcccaac ccgcttgaag gaatttaatc tctgtgcccg
     1381 gataacgttt ttctacgttc atcaagatcg gggtgaggtc gaccacgttg ccttccatcc
     1441 cgaagatggg cttgatcttg tgcgcctggc agcgttcata gaattcgggg aaggcttgga
     1501 tgacctcgtg atcggttatc ccaacggctt tttgccctga cttcgcggcc cgttcgacca
     1561 aggcttccac ggacaagatc gagtccatcg cgctcagatg cgtgtgggtg tgtaactgaa
     1621 cgtgagcggc ggttacagac gagggcgtcc gctcgttaac ggtttcgaca tcagacgggt
     1681 cttcggcagt tacgcttacg gacgcactat cgggatcctc gatctcgcta ctttcggctt
     1741 cgagcgtcgg cgctccttct ccttcgttcc tctcgtcctc ggaatgaacg gtaacggata
     1801 agaccgtctc gatccgggcg gggaggcaga agaaccgggc aaaggctttt tccataaaag
     1861 aaaagtgcgc ggcgtctagg gagtcggggt tgtgggagat ccggacgacc acttgcgcct
     1921 tcgtcagtac gaccgacaga accttcccct cgggggctga ttccgctatc cccagccttc
     1981 ctaagacctc agaaaggggg tgatcgatcg attcgatgac gtatttcata gagggcctcc
     2041 cggtcgcgaa ggggtcgagg ggttgagata ggccgaaatc cctttcgcga cttttctgga
     2101 attcgcgtga caaaaggccg cggctaacgc gtcagaggcg tcgtcttgat ggagtgtgcc
     2161 tttcaagttc agcaacaacc cgagggttct ttgaacttgc cccttttctg cgtgcccgtt
     2221 gccggttacc gattgcttga tctcgaacgg ggtatattcg aagatcgata cccggtgttg
     2281 atacagcgcc agttcgatta cccctcgtgc ttcgctgacc ttcatcgcgg ttgtgacgtt
     2341 tttaacgaaa aacagtcttt ctatcgcggc ttcatccggc tcgtgggtct cgcagatcgc
     2401 gcggatttca gaatagatca acgcgagccg ctcggggaaa gacgcctctc gtggggtttc
     2461 gatcagtcgg tggaaaagcc aggagtagcg gtttccttct acttcgatca gaccgacgcc
     2521 tacgcgcccg taaccggggt cgataccgat gatgcgtagg gggttcatcc ttcccccacc
     2581 gggatagcgg tttcgttttc gtacaaaaag gcatacaggc ctttcaacag ttcatccagc
     2641 tcggtggaca tcaataggcg gtttcgggtg ggtcgcgcca tcggtattcc cttcgtgtag
     2701 ccgacaacga acttcttaaa ggcgccgcat atcctatctc tgcgcatctc tccctccatc
     2761 aaggcgatgt ggcgctcaat ggcgcaaacg cgctcttggg cgtcgggcgg gcggtagtct
     2821 ttcccaagga tcgcgctctt cagctctcgg aaaatccaag ggttaccgat ggaagccctc
     2881 ccgatcaaca ctcccgctat gggaaaacga gcgactctcg cgagcgaagg ggggtcaacg
     2941 atatccccac tccacagaac gcgagaacac tccgcttgcg gtatttcatc taaaaccgtc
     3001 aggtctgccg ttccagagta tccctgttcg acggttctac cgtgtagtgc gaccgcgtcg
     3061 actccacaac cgatcgccat cgtgatgacc tctcgatagt tcacgtttgc ctcccacccc
     3121 ttacggattt tcagtgtcag ccgccgtccg gcagcgtgcg cgggaggttc cgctgcctcc
     3181 aagatacggc gcaaggttgg gagatctttc gccaatgcgg ctcctgcgcc gttccgcacg
     3241 actttccgga ccgggcacgc cgcgttgatg tcgattccta cagcattcgg ggcgcgggcg
     3301 agaacgatct cgatcgcttt cggaaagtcc gcaggatccg caccgaacaa ctgaatgacg
     3361 gccctttcgc ctaatttcgg caaagtccga aagcttccgc gattcttgtt cagtagccct
     3421 ttcacgctga ccatctctgt gtaaaacccg tccacgcaat cggggacggt ggtccgagtc
     3481 aattcccgga aggcgtagtc cgtcaccccg gctaatggcg ccaaaattag cggtacatcg
     3541 cgtattccta ctatctgaga caccttctcc cgtctttttt tgtcacacct attgtacagg
     3601 aagcaggtaa aatttctgag aagagaacgc gcgggggcga gaccggtatt ctatcaaaaa
     3661 gaggcaagtc ggtttattga cgtggtgtgg gccaagttat ggggtgactt aactaagagg
     3721 ttcattgctg ggtagccagc gggctgactt aggaagatca cgaagtctcg cgatcttcct
     3781 tttttctttt ttacgccgcg atgcccgtat gaggttatcg cggggtaacc agagggctga
     3841 cttcggaagt tcacgaagtc tcgcgacccg ctctggtgcc tttctctttt ttttaaccat
     3901 gccaaactaa agtttggcgt ttccaggcgt ttccaaaaat cttttctctg tgttctctgt
     3961 gccctccgtg cgaaccctat ttttcagaac ctgtacaaaa acgtatcgcg ccgtcgcgaa
     4021 aagaagaagc gcttacgccg cgataaccgt gtgaggctca tcgcgaggaa gccaacgggc
     4081 tgacttcgga aactcacgaa gtctcgcgat ccgccttttg tcttttttac gccgtgatgc
     4141 tgcgcatgga ctacaaatcg cacgatcgat tctggccttg tttcttgatg gcacctcata
     4201 acttgacacg cactttcttg acgtgtcggt gttcagggag agtcgctgat ggtggcggac
     4261 ctgaattcgg cttctctcta taccccgtgc gcggccttga cgaagcatcg gttatatggt
     4321 aaaataactc cgctgttctg gcaggagtag cttcaatcgg tagagcgccg ccttggtaag
     4381 gcggaggtta tgggttcgag tcccatctcc tgctccaata cgctcaaaag cgatagaccc
     4441 ccgaaactaa ggaaagatgg ttgtggaggg ggtttcttct tattatgaat cacgatttat
     4501 cgtgtctcgc gaaaggacgt gcgctcattc gatcaggaat cgtcacgctt gtcggaaaac
     4561 cgaataccgg aaaatcaagc ctcatcaacg cgatcttcca gcagaaggtc gtgatcgtct
     4621 cggataaacc gcagacgacg tggcatcaca tccgttgtgt ctacaccgac ggcacctcac
     4681 agatcatctt ttccgacacg cccggggtaa acaagcccat tggcccgttg ggcaagtata
     4741 tgacgaaggc cgtgagaagc gctttggagg ggtctgacct cgtcctgtgg ctcttcgact
     4801 gctctcgcgg ggtgaccgaa gccgaacggt acgtttatga taaaatacaa tcgatcgaac
     4861 ccccggtctt tgccgttctg aacaagatcg acctggcggg accgactgaa atcgataaag
     4921 ttttagccga tatggccgaa tttggccctt tcgcggagac tcaccaaacc tcagcgacga
     4981 ccggtgtcgg cttggctcgt ttgatggaag ggatcaaaat ccgcctaccg gaaggggact
     5041 ttctctacga ccccgaggtc ttgatcgaca aaccgatccg gtttatcgtt tcggaattga
     5101 tccgagaaaa gatataccgg tacacctcca aggagattcc gtacgaaacg ggggtgtaca
     5161 tcgaacacct cgatgagaaa ccgtcctcgg gcctctccat ccgcgcggag atcttcgtca
     5221 gccgtgacaa tcacaaggga attctgatcg ggaagggtgg agagatgatt cggaagatcc
     5281 gcctatcggc gaaacgcgac attgaaatcc tcttcgatcg caaggtggga ctggagctct
     5341 acgtacgcgt taaagaacgg tggatgcacg acgagaacct gaggaccaaa gatctcgact
     5401 tcgaaacgat cgttcgaaag agcaggtgaa cccttggaac cgaaaactcc gctctcttcg
     5461 gcttctctct cgtcgttgac ggagatcatc gggccgtttt acttcgtggg cggatacgtc
     5521 cgcgacctgt tgatgcgtcg aaggtcccgt cgacaccata aaggtgcaat cgacatcgat
     5581 atcgccgtcg attctcctat ggagacggtc cgcgaacggt tggcgaaggg ttttttttcg
     5641 aatcgagaga agatgaccct cacgtttttt tggaagggtc tgaccatcga tctggtctcc
     5701 ctcaatggcc tgacgatcga cgaagaactg acgcgtcggg atttgaccat caattcgatg
     5761 gggctttctt gtcaaagggt ctttgatatc ctcaaggagg agcccgactg ggaagggtcg
     5821 atacggcctg aggatgtgat cgatcccacg gggggatggt cggacttacg ggcaaaaatc
     5881 ctgcgctgtc cacgggaaaa gaatctgacc gatgatccgg ttcgcttggt gcgcgttttc
     5941 cgattccaag gggaattcgg attcgagata gaggatttga cctttcaatg gatccaccgg
     6001 cacgccccga cggttcggtt ggtggcgaag gaacgggtgt cgcgagaact cgaaaaactg
     6061 ttctcttcgc cgtatgtcgt gaaggccttg cgagagctga agaagacgcg tttgttccgc
     6121 gagcttttcc ccttactgac ggggccgctc gaccgcagac tcgaggaccg gccgtatttt
     6181 ggccagagcg tcttcgaaca cagccttcgc gtgatcgctg gctgcggctc cgatcccttg
     6241 ctaaaatggg ctgggttttt ccacgacgtg ggaacacgag acggtttttc ccgcgtatcg
     6301 tctacggatg aagaaaccgc gccccgtgac aattatcacc cggaggagag gagtgtcgag
     6361 ctcctccttg agacggccaa agattggggg ctgccgaaga gaattgtcga tcccgtggaa
     6421 aagatcatcc ggttccacca ggttcctgtg acctcgcgaa gcgcgattaa ggaagcgagt
     6481 atgcattttc gcgagccgaa agcccacaat cgatttttca ggttcgtcca agcggacagg
     6541 tgcgccgccg atccgctttt cttagaaacc gacgagggga aggctcacaa agagatcgtg
     6601 aaagcggtgc gccggatgat gccgaagatc agccgttgtg agcgtttttt ctctgggaag
     6661 actctgctgg ctttgggttt acgcccgaca ccggctttca aagttctcat cgaccacttg
     6721 atgaaaaagg ctttggaatt ggacttcaac ctcaccgaac ggacggctca agaacttttg
     6781 gagtccacgc tgcgatttaa ggaacgctac gagcggcttt ttttcgataa tacggagtat
     6841 aggaggaaac gagaatacta catcgacgga gaagtcatag gggttctcga agaggatgtg
     6901 aaactgacgg tgcgcaccga ggtctttctc taccgtttcg aacccgaggc gatgttaccg
     6961 gaggcgctca gaggcaaaaa tcccctcttg ttcttttctt tgtcgtcctg attggcctcg
     7021 cttgcggcct tatggcccaa gaaaccgcca ccgtctacta cggcgcgcag agcctttacg
     7081 attttcagaa agaaccggaa tggctcgaga ggacccggga acgattcttg aaaaccttgg
     7141 tggagcaacc cgacttactc gaatcggaag agacggtcga cgcgctcggg accaggatct
     7201 atatgaagtt gcggctcttg cgggattccg acctgaaagg cccggttcta acagccattt
     7261 tagccgagaa ttcggaggag tcgcttcggc tgctcgcttt ggaagaagac cgcctatcgc
     7321 ggtatcgacc gctctatcca ctgatggact ttttcgtaaa agacttccga aaagccctta
     7381 accctttcga ttttcagaaa atcgttccgt atttcttgat cggacagtta gaagggttcg
     7441 aagagttttt ttctcttacg aatgaagagc actaccacat tgcgaaaggc atcctgaacg
     7501 gaatccaaag caatcgaacc taccccgaga ggtctttaaa ggcgatggta aagttgctgg
     7561 gtgaaaaata cctccgggct tacgcgtcga tggttcaaca gttggtgacg gcgagggtcg
     7621 aggccgacta cgccaaagaa ctgcgcctgg ccaagtctta tgtggaccag gccggttacc
     7681 tgtacggggc tcaggtcagt caaaaggcgg accagacgct ccatcgggct tacctctcgt
     7741 tgtcttcgta cgcttcctgt aaagaagata ccctgatcgc ggagaaccgc gtggcattgg
     7801 cctcccagga tacctacctc tctatgttgc ccctcgctat ggaactcgta gaccgctacg
     7861 aacgcctgac gatcaataaa gaaggactgg aaaagctcat tctctctttc atacaaaact
     7921 acggtttgaa aagccggctg tacggcgatg aggtcaccgc cgaaatccga aggaaatgga
     7981 aaggttttat cgacgaacgc gacctctctc cgatggttct caacgcgatc cgctcgggaa
     8041 ttttggacta tgccgctaaa actccccaaa ctgtcgcgac accggctgtc gcgacaccgg
     8101 ttgtcgtgac cacgaaaggc tccgtgaaag gctcttggtt agagaagttg cgatggccca
     8161 cagtgtttat ttacggagtg cccgttttgg ctttgttact ctttatcgcg gttttaccgg
     8221 cctcgttgaa ggcgaaggga gctaaagccc tcggtatgaa gaaaaaagcg ttggccttct
     8281 atcagaaggc ttccctttcc aaacccgagg tggccgaata ccacgtccag atggccatcc
     8341 ttctggggtt gctgcaccga gaagaagagg cgatacaaga ataccggatc gcgcctaaga
     8401 tcctcagtat gaccgaagac gacgacct
//
LOCUS       DAOPXN010000091         8412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8442_length_8412_cov_4.760799, whole genome shotgun sequence.
ACCESSION   DAOPXN010000091 DAOPXN010000000
VERSION     DAOPXN010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8442_length_8412_cov_4.760799"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PKM49_06240"
     CDS             complement(<1..732)
                     /locus_tag="PKM49_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNY82450.1"
                     /translation="MKCVVLLSDGIDSAIAAHLLKAVGHTVLGLHLVKGNPEGDRVGG
                     EGGQALKTSAAAEAEKHARELELDDFLCLDITEEFEAEIIRDFLNGYRRGLTPNPCVR
                     CNRLIKFGRALELAKRLGADKLSTGHYIRCEYSEKYRTHVLRKGADSSKDQSYFLSSI
                     RKEVLPMLTFPLGPLQKREVRVIAQRLGLMFSEKKDSQEICFVPGDSYQKFLAARGIP
                     ESPGEIYDHQGKRVGAHTGYQNYTIG"
     gene            1264..2253
                     /locus_tag="PKM49_06245"
     CDS             1264..2253
                     /locus_tag="PKM49_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HNY82451.1"
                     /translation="MRIREKVKPRVLKRGDTIGIVAPASPLYNKSDLVRGAETLSEMG
                     YKPLFGKTVYKRREYLAGTDQERAADINAMFANKKVNAIFVTQGGYGSARIIKYLDFS
                     VIRKNPKIFMGFSDISILHLAIQKKTRMVTFHGPGMAGFNDSELTDYRKEYLMKALTT
                     VTPIGEIRPTSPRQYIHSIYPGQVEAEIVGGNLALICSTLGTPWEIDTKGKILFFEEI
                     DTEPWMIDHLLTHLSNAGKLDEVAGVVVGECINCIPYSHHPGFPVTFSLEDILTEFLE
                     PLKKPAIYGLPMGHSKDMATFPLGCRAFLDADEKKLFITESGVSEQNQTVVVP"
     gene            2277..3887
                     /locus_tag="PKM49_06250"
     CDS             2277..3887
                     /locus_tag="PKM49_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82452.1"
                     /translation="MFATPFLRLSKRVGLFFLIGLLSISAFSAALKVGWTVEPDSVSP
                     FIAYNLPSAEVFLLLYDPLVALNEKEEPVGKIAESWEISEDQLTWTFYLRKGIRWHDG
                     EPLRAEDVKFTYDLFMNSGLGLFSGFLNGIESIECPDDYTVLIHSEEPKANMLMGNTP
                     ILPKHVWEQVAEEDLELWENEPAIGSGAFTFLEWRKGEFLRLKANRAYALEKPKVDEV
                     VFILFANNDTLAQALKTGEISAAVNINPMQLPQLSLSPNLLAVSSPGYGFTQLAFNCW
                     ESEDSLGHPALLDKRVRQAIDWALDKKAILDMTYLGQGEVGTTLIPPILQKWHYRLTD
                     TERRDYNPKKANDLLASSGYPIGPKGIRVDPQGNELRFRLYLRASNTQEIKAGQLIKK
                     YLQAIGIDAAIETVDDGLLMDLIFSNADFDMFIWGWGTDVDPTTILNVMSTNEIFGLS
                     DCFYSNEEYDRLLALQATQMDEAQRRETVYEMQRILYEDSPYAILFYDNEFQAVRTDL
                     FSGWKRIPENGAFFFTINPYNYLNVTPK"
     gene            3929..4897
                     /locus_tag="PKM49_06255"
     CDS             3929..4897
                     /locus_tag="PKM49_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82453.1"
                     /translation="MSSRYLGNKIAQALITIFIVLCVNFFLFRIMPGDPLSMLMRNPR
                     ASQQAIQKAKELYGLDQSLPAQFGRYLQDLVKLDFGVSFMFKKPVVEVIGGKIGPTLL
                     LLGVSELIAIILGIYLGIVAASHRGRIVDVGAISLSLLLYSLPTFLLGMVLILVFSVY
                     WGLFPSVGMVTAAAKFSSWWRQSLDLLAHLFLPAMTLALTLIGEYGMLMRSSLIEVLS
                     EDFIVTARAKGLSEREVIHKHGYPNALLPVVTAISINLGLTIAGAIQVETVFSWPGIG
                     RLMYDALNNRDYPLLQGIFLLVCICVIVANLLSDLLYMVLDPRVKS"
     gene            4899..5774
                     /locus_tag="PKM49_06260"
     CDS             4899..5774
                     /locus_tag="PKM49_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82454.1"
                     /translation="MPLDKPTLSLRFKRAFSVLYGNKIGFTGFLILCFFFFIALFGPW
                     IKPYDIMDFGGAADILRPPSLEHLLGTDDMGRDILGGLIAGSRISLLVGVMATVLSMF
                     LGAVIGLTAGYFGGFIDAVLMRLTDIFLVLPWLPLVLVLAAILGANLWNIILVIGLTS
                     WAGTARVVRAQTLSIKERTYVERARSLGSSNLRVILRHILPNVFPVIFANTVLIAAVA
                     ILSETTLSFLGLGDSLNQSWGMMLHYAFECGAASIGAYWFLLPPGLCVVLVVLGFTFL
                     GYALDEWINPQIRKR"
     gene            5788..6783
                     /locus_tag="PKM49_06265"
     CDS             5788..6783
                     /locus_tag="PKM49_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014737835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82455.1"
                     /translation="MGTVTPLLEVRDLKTYFHTKEGIAKAVDGISFEVNEGASFGFVG
                     ESGCGKTTAALSIIKLLPKNGRIEGGQVLFRGEDLTAKSDEEIRMRRWKDVSIVFQGA
                     MNSLNPVMRISKQIVEAIRLHEDISIPEARERVKRLFAQVGLNPALLDQYPHEFSGGM
                     KQRVMIALALACNPRLIIGDEPTTALDVMVQAQILDLLEELRRSLNLATILISHDLSV
                     IFDACERLAVMYCGKIVEIGGAREIYLRPAHPYTRKLVNAFPDLELEKQRKTGKRALG
                     KSIAGDPPNPLDRITGCKFHPRCEWAEPRCVEEEPVAMKVGADHRAFCWKWDRIG"
     gene            6776..7774
                     /locus_tag="PKM49_06270"
     CDS             6776..7774
                     /locus_tag="PKM49_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82456.1"
                     /translation="MDNTLVTIQNLSVVYTLKTSFAGWLKGNKKRTLQAVDDLSLTLE
                     KGEILSLVGESGSGKTTTGRALLRLIKEKVRGSIHFEGRDVFSLNGRELKEFRKKAQM
                     IFQDPYQSLNPRDTVSRIVGESLDIHHRELSAEKKREMVTQALEWCGLKPVERFWNRF
                     PHELSGGQRQRVAIAGALILKPQLVVADEPVSMLDASVRLDILGLLNDLRAQIGVSCL
                     FITHDLALAWLISDRIAVMYAGAIVELGSAELIAQGALHPYSKALIAAQPSATATKER
                     KRAVLVDREVDPVAKTFCRYYRRCPLATSQCLEEAPGLLEVHPGHFVRCFNWRTDD"
BASE COUNT         1871 a         2178 c         2310 g         2053 t
ORIGIN      
        1 gcctatcgtg tagttctggt agccggtatg cgcgcccacc cgcttgcctt gatggtcata
       61 gatttctccc gggctttccg gtattcctct ggcagcgaga aacttctgat aggagtctcc
      121 gggaacgaag catatttcct ggctgtcctt tttttcagag aacatcagtc cgagcctttg
      181 ggcgatcacc ctcacttccc gtttctggag tggccccaag ggaaaggtga gcatcggtaa
      241 aacctccttg cgtatcgaag agagaaagta cgactggtct ttcgaagaat ccgcaccttt
      301 tctaaggacg tgggtccggt atttctcaga gtattcgcag cgtatatagt gacccgtgct
      361 cagtttatcc gccccgagac gttttgccag ctcaagcgcc cgtccgaatt tgatgagtcg
      421 gttgcatcgg acgcacggat tcggcgtcaa cccacgtcgg tagccattca aaaagtcccg
      481 aatgatctcc gcttcgaatt cttcggtgat atctaggcat aggaagtcat ccagttccaa
      541 ctctcgggcg tgtttttcag cttcggctgc ggccgaagtc ttaagagcct ggccaccttc
      601 tccgccgacc cgatcgccct cgggatttcc tttgaccaaa tgaaggccta gaaccgtatg
      661 tccgaccgct ttcaatagat gagccgctat ggcgctgtcg atcccatcgc ttaagagtac
      721 aacgcatttc atcttttcac ccgtttcttc ttgatcatcg acaccctatt gtacaacaaa
      781 cctacatctc ctgaatgtct atgcgtgtgt caagttaaga ggtgtactca gaaaacaagg
      841 ccagaatcga tcgcacgatt tttttccttg tagtcatcgc ggcgtagaag gggctaaggg
      901 cggatcgcgg ggtcgtaaaa accacttacc ccgcgataac acacgcgaac atcgcggcgt
      961 aagcgccact tcttttcgcg acagcgcgat acgcttttgt gtgatccgat cgcgggcgtc
     1021 ttaaaaggag aaagagcaga tcgcgagact tcgcaggctg ggaacgccga actttaattt
     1081 ggccttgttt tatctttcac tagaagcgtt cgtatgcgct gcatcgcggc gtaagcgctt
     1141 cttatttttg cgacggcgcg atacgctttt ttaccggctc atcccgtgac ttcgtgggcg
     1201 aagagattcg ttcccgctcg tggtataatc gtattattaa tgcatagttt cgtaggtgaa
     1261 aagatgagaa ttagagaaaa agtgaaaccg agggtgctca aacggggcga cacaatcggc
     1321 atcgtcgctc ccgcaagccc cctgtataac aaaagcgatc tggtgcgcgg cgcagaaacc
     1381 ctcagtgaga tgggatacaa acccttgttc gggaaaacgg tctataaacg aagagagtac
     1441 ctcgccggaa ccgatcagga acgcgcggcc gatatcaacg cgatgttcgc gaataaaaaa
     1501 gtcaacgcca tcttcgtgac ccagggagga tatggctccg ctcggattat taaatacctg
     1561 gacttttccg tcataagaaa aaacccgaaa atctttatgg ggtttagcga tatttcgatc
     1621 ttgcacttgg cgatccaaaa aaagacgcgg atggtcactt ttcacggtcc cggtatggcg
     1681 gggttcaacg actctgagct gaccgattat cgtaaagaat acctgatgaa agccctaacc
     1741 accgtcacgc caatcggtga gatacgcccc actagcccga gacagtatat ccattcgatc
     1801 taccccgggc aggtcgaagc ggagatcgta ggtgggaatc tcgccttgat ctgttcgaca
     1861 ctcggaaccc cgtgggagat cgacaccaaa ggcaagatct tgttcttcga ggaaatcgac
     1921 accgagccct ggatgataga ccacctgtta acccatttga gcaacgcggg gaaattggac
     1981 gaagtggccg gcgtcgtcgt cggagaatgt atcaactgca ttccgtattc gcaccacccg
     2041 ggctttccgg taaccttcag cctggaagac atcctgactg agtttttgga gcccttgaag
     2101 aaacccgcca tctacggatt gcctatgggg cattcgaaag atatggcaac ctttccgctc
     2161 ggatgccgcg cgttcttaga tgccgacgaa aagaagctct tcatcaccga aagcggcgtt
     2221 tccgaacaga accaaacggt cgttgtacct taaatcctta atagggaggg aatggtatgt
     2281 tcgctacacc atttttgcgt ttgtcgaaga gagtggggct tttcttttta atcggccttt
     2341 tgtccatttc ggcgttttcg gctgccctaa aggtggggtg gacggtggaa ccagacagcg
     2401 tcagcccgtt tatcgcctat aatctcccgt cggcagaggt ttttctgttg ctgtacgatc
     2461 cgctggtcgc tttgaatgaa aaagaggaac cggttgggaa gatcgccgag tcttgggaga
     2521 tcagcgagga tcagctgacc tggacctttt acctgcggaa ggggatccgg tggcacgatg
     2581 gagaaccctt aagggcggag gatgtgaagt tcacctacga cttgtttatg aacagtggtc
     2641 tcggcttgtt ttccggattt cttaacggga tcgaatcgat cgaatgcccg gatgattaca
     2701 cagtcctgat ccactccgaa gaaccgaaag cgaatatgct gatgggcaac acaccgatcc
     2761 ttcctaagca cgtttgggaa caggtagccg aagaagattt agagctgtgg gagaatgaac
     2821 cggcaatcgg atcgggcgca ttcaccttct tagaatggcg caaaggggaa tttctccgct
     2881 tgaaagccaa tcgagcctat gccttagaga aaccgaaggt agatgaagtc gtgttcatac
     2941 tgttcgccaa caacgatacc ctggcgcagg cgttgaagac cggggagatc agcgcggcgg
     3001 tgaacatcaa cccgatgcag cttccgcagc tttccctatc cccgaatttg ctggcggtct
     3061 cttctcccgg atatggcttc acccagctcg ctttcaattg ctgggaaagc gaggactctc
     3121 tgggccaccc cgcgctgcta gacaagcgag tccggcaagc gattgattgg gctctcgata
     3181 agaaagccat tctggatatg acctatctcg gtcagggcga ggtgggcacc acactgatcc
     3241 cgccgatcct ccagaaatgg cactatcggt tgaccgatac agagcgtcgg gattataatc
     3301 ctaaaaaagc caacgatctc ctcgcctctt cgggttatcc gataggcccg aaaggaatcc
     3361 gcgtcgaccc tcaagggaat gagctgcgct ttagacttta cctgcgcgcg tcgaacacgc
     3421 aggagataaa agccggacag ctgatcaaga aatatctgca ggcgatcgga atagatgcgg
     3481 ccatagaaac cgtcgatgac gggttgctga tggacctcat cttttctaac gcagatttcg
     3541 atatgttcat ctggggctgg ggcacggatg tcgatccgac aacgatcttg aacgtgatgt
     3601 cgacgaacga aatcttcggg ttgagcgatt gcttctattc caacgaagaa tacgaccgtt
     3661 tactcgcgct tcaagcgacc cagatggatg aagcccaaag gcgagaaacc gtgtacgaga
     3721 tgcaaaggat cctctacgaa gacagtccgt acgccatcct tttttacgac aacgagttcc
     3781 aagcggtcag aaccgacctc ttctcgggat ggaaacggat tccggaaaac ggcgccttct
     3841 tcttcaccat taacccatac aattacttga acgtgactcc aaaataacct gaacgggcga
     3901 gagctcttcg tgaggggtgt tgctgttcgt gtcgtcacgg tatttgggca acaaaatcgc
     3961 tcaggctttg ataacgattt ttatcgtgct ctgcgttaac ttcttcttgt tccgtattat
     4021 gcccggggac cctctgtcta tgctaatgag gaatccccgg gcttcacagc aggcgataca
     4081 gaaggccaaa gagctctacg gattggatca atccttacct gcgcagttcg gtcggtactt
     4141 acaggacctc gtcaagctag acttcggcgt ttctttcatg ttcaaaaaac ccgttgtgga
     4201 ggtgatcggc ggaaaaatcg gccccacgtt gctcttattg ggggtttccg agctgatcgc
     4261 catcatcttg ggcatctatt tggggatcgt agccgcctca caccgaggaa ggatcgtgga
     4321 cgtcggcgcg atcagcctat ccttgcttct gtactccttg cccacctttt tgttggggat
     4381 ggtgttgatc ttggtgttta gcgtctattg gggcctgttt ccttcggtgg ggatggtgac
     4441 ggccgcggct aagttttcct cgtggtggcg tcaaagccta gacttgttgg cccatctatt
     4501 tctacccgcg atgacgttgg ccttaacctt gatcggagag tacgggatgc tgatgcgtag
     4561 ctctctgata gaggtgcttt ccgaggactt catcgtcacg gcgagggcga agggcttgag
     4621 cgagagggaa gtcattcaca agcacggtta tccgaatgcg ctgctccccg tcgtcaccgc
     4681 gatctctatc aatttgggcc tcaccatcgc cggagcgatt caagtggaaa ccgttttctc
     4741 ttggcccggg atcggccggc tgatgtacga cgcattaaac aatcgagatt acccgctgtt
     4801 gcaggggata tttctattgg tctgtatctg cgtgatcgtg gcgaaccttc tttccgattt
     4861 gctctatatg gttctcgatc cgagggtgaa gagttaaaat gccgctcgac aaacccactc
     4921 tctccctacg gttcaagagg gctttttcgg ttctgtatgg aaacaagatc ggtttcaccg
     4981 ggtttttaat cctgtgcttc tttttcttca tcgccctctt cggtccgtgg atcaaaccct
     5041 acgatattat ggacttcgga ggcgccgctg atatcttgag gcctccttcg ctcgaacacc
     5101 tcctgggaac ggacgatatg ggacgcgata tcttgggcgg gctcatcgcg ggttcgcgaa
     5161 tatctctgtt agtgggcgtg atggccaccg tgttgtcgat gtttttggga gccgtcatcg
     5221 ggttgaccgc cggttatttc ggcgggttca tcgacgccgt tctcatgcgg ttgaccgaca
     5281 tcttcttggt actcccttgg cttccgttgg tcttggtgct ggcggccatt ctcggggcga
     5341 acctgtggaa catcatcttg gtcatcgggt tgaccagttg ggccggaacg gcgcgggtcg
     5401 ttcgcgccca gaccttgtct atcaaagagc gtacctatgt ggaaagggcg cgttcgttag
     5461 gctcctccaa cctccgggtg atccttcggc atatcttacc gaacgtcttt ccggtgatct
     5521 tcgccaacac cgtattgatc gctgccgtgg ccatcctctc tgaaacgaca ttgagctttt
     5581 tgggcttggg ggattcgcta aaccaaagct ggggaatgat gctccactac gctttcgaat
     5641 gcggcgcggc gagcatcggc gcctattggt tcctcttacc ccccggcctt tgcgtggtcc
     5701 tcgtcgtgct cggtttcacc ttcctcggct atgctttgga tgaatggatc aacccccaaa
     5761 tcaggaaacg gtaggagggg aaggcgattg ggtacggtta cacccttact ggaagttcga
     5821 gatctgaaaa cctattttca tacgaaagag gggatcgcga aagcggtcga cggaatttcg
     5881 ttcgaagtga acgaaggcgc atccttcggg ttcgtgggcg aatctggctg tggaaagacc
     5941 acggctgctc tctcgatcat caagctcttg ccgaaaaatg ggcggatcga agggggtcaa
     6001 gtgctcttcc gcggagaaga cttaaccgcc aagtccgatg aggaaattcg tatgcggcgg
     6061 tggaaagacg tctctatcgt ctttcaaggg gcgatgaact ctctgaatcc ggtgatgcgg
     6121 atcagtaagc agatcgtcga agcgatcagg ctgcacgaag acatctcgat acccgaagcg
     6181 agggagcgcg tgaaaaggct cttcgcgcaa gtggggttga acccggcctt gttggatcag
     6241 taccctcacg aattcagcgg cggaatgaag caacgcgtga tgatcgccct ggctttagcc
     6301 tgcaatccgc ggctgattat cggcgacgag ccgacgaccg cgctggatgt gatggttcag
     6361 gcacagattt tggatttgtt ggaagaacta cgccggtcgc tgaatttggc cacgattctc
     6421 atctcacacg atttgtcggt gatcttcgac gcttgcgagc ggttggcggt gatgtattgt
     6481 ggaaaaatcg tggagatcgg cggtgcaagg gagatctatc tccgccccgc tcacccttac
     6541 acccgtaaac tggtcaacgc tttcccggac ttggagttag agaaacagag aaagacggga
     6601 aaacgcgcct tgggcaagtc catcgccggc gacccgccca atccgttgga ccgtatcacc
     6661 ggttgcaagt ttcatccccg ttgtgagtgg gcggaacccc ggtgcgtcga agaggaaccc
     6721 gtcgcgatga aagtcggggc cgatcatcga gccttttgtt ggaagtggga tcgcattgga
     6781 taacacgttg gtcacgatac aaaacctgtc cgttgtttat acccttaaaa cctctttcgc
     6841 cggatggttg aaagggaaca aaaagagaac gctccaagcg gtcgacgacc tgtctctcac
     6901 gttggaaaag ggtgagatcc tttcgctggt cggagagagc ggtagcggca aaacgaccac
     6961 ggggcgagcc cttctccgac taatcaaaga aaaggtgagg ggatcgattc atttcgaggg
     7021 tcgagacgtc ttctccttaa acggtcgaga gctgaaggag tttagaaaga aagcccagat
     7081 gatcttccag gacccgtatc aatcccttaa tccgcgcgac accgtctccc gaatcgtagg
     7141 agaatccctg gatatccacc atcgggagct gtctgcggaa aaaaaacggg agatggtcac
     7201 ccaggctttg gaatggtgcg ggctgaaacc ggtcgagcgg ttttggaacc gttttcccca
     7261 cgaactgagc ggtgggcaac ggcaacgggt cgccatcgct ggcgctctca ttttgaaacc
     7321 ccaattagtg gtcgcggacg aacccgtgtc tatgctcgac gcctcagtca ggttagacat
     7381 actcggttta ctgaacgatt tgcgggcgca gattggggtg agctgcctgt ttatcaccca
     7441 cgacttggct ttggcttggc tgatttcgga tcggattgcg gtgatgtacg cgggtgccat
     7501 tgtggaatta ggttctgccg aactcatcgc acaaggggct ttacatccct attcgaaagc
     7561 gctcatcgct gcgcagcctt cggcgacagc tacgaaagaa agaaagcgcg cggtacttgt
     7621 tgaccgggaa gtagaccccg tagccaagac gttttgccgg tattaccggc ggtgcccgct
     7681 agcgacgtca caatgcctcg aggaagcgcc agggcttttg gaagtccatc ccggtcattt
     7741 cgtacggtgt ttcaactggc gaacagacga ttgatcaaac cagacggaac atatcacgaa
     7801 cgcgtgtgga acaagttatg gggcgacata actaagaggt tcatcgcggc gcttacgccg
     7861 cgttgttacg cttgggaaca ccttcggggc gccgccagag cggcggactt tagtctggcg
     7921 tgtttttttc agacactctc gggaaaaacc tgccaactta aaagttagcg ttcccaaggc
     7981 cttctcggag agagaggccg accggaagtc ggccccacga gctttcttca gtcttttctc
     8041 tgtgttctct gtgccctctg tgcgaacccc cgtttttacg cgccgtcgcg agaagaagga
     8101 tcgcttacac cgcggtgacc gtgtggggtt atcgcggggt aagtggtttt tacgaccccg
     8161 cgatacgcat tttattttta agacgccgag atgcccgtgt gaggttatcg cggggtaacc
     8221 agctggctta cttcggaagc tcacgaagtc tcgcaatacg ctctgctgcc tttctttctc
     8281 aagacatgcc aacttaaaag ttggcgttcc cagggccttc ccggagagag aggccgactg
     8341 gaagtcggcc ccacgagctt tcttcagtct tttctcggtg ttctctgtgc cctctgtgcg
     8401 aacccccgtt tt
//
LOCUS       DAOPXN010000092         8285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8621_length_8285_cov_4.861847, whole genome shotgun sequence.
ACCESSION   DAOPXN010000092 DAOPXN010000000
VERSION     DAOPXN010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8621_length_8285_cov_4.861847"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..281
                     /locus_tag="PKM49_06275"
     tRNA            206..281
                     /locus_tag="PKM49_06275"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Ala,seq:cgc)
     gene            286..374
                     /locus_tag="PKM49_06280"
     tRNA            286..374
                     /locus_tag="PKM49_06280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:320..322,aa:Leu,seq:caa)
     gene            525..1154
                     /locus_tag="PKM49_06285"
     CDS             525..1154
                     /locus_tag="PKM49_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNY82457.1"
                     /translation="MLSRKKRGKRPLDTERNGDNLFVCALREKRTWAYEKLYDEYAGL
                     IGSIAKSYLNVDDVDDVIQEVFLRVYKSIKQFKGDSTFSTWLYRIAVNVCKDQLKKYA
                     KRSETMTDFDSEEPGQLEPVADHNVEIAVEKQFDSEDMEAILKRLSAEDQELIRLRDV
                     QDLEYEEIASIVEKPLGTVKSKIHYARKRLKKLLTTYYGLREEENQGGE"
     gene            1154..1633
                     /locus_tag="PKM49_06290"
     CDS             1154..1633
                     /locus_tag="PKM49_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNY82458.1"
                     /translation="MNCKLVREEYMDRYLDKELTFEEIKELKAHLEGCPQCQKAFSTY
                     KTFIQMQKARISFSPSAHGKQKLVKRIRQKKLLPYQLAGSVAVAFLCFFAVTAFIESR
                     IINQRFEKILSTTIHSITPTSNASMTISGTPRNDKKSISPQDYVDPERILYIVNDGD"
     gene            1668..2726
                     /locus_tag="PKM49_06295"
     CDS             1668..2726
                     /locus_tag="PKM49_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82459.1"
                     /translation="MGKGRSLLLFGIFFYFLTLTSSLYGFQITGATSPDSSGNAHEYI
                     AGLLKAYESQSVQGTRIFLESGSKNRFRYEYFIRSADQKIVTLKMPFEVTWFRFEDEG
                     YLVQNGTKTKVDVPLKDLEDLLIEKLKSCPITFSVSEVLYTGLPASFVVINLPDHSQY
                     RLILMRDSYQFIKIEYEKADSLSIMLYEHLFPYEREVFSEDMNFYRSLPFWDDGTKTT
                     DASIANSDGLGEGNAGPLSAHGALYYDFERFITHLVRFYTLANIEAQEHPGYSVLMVK
                     LELEGDAAPVALLLFRSKDPRNPSRIPEALLGTIPSDYEIAHTEVGRYTIYVFGEAEK
                     ARLESILDGLAGQAAEED"
     gene            complement(2731..3138)
                     /gene="cdd"
                     /locus_tag="PKM49_06300"
     CDS             complement(2731..3138)
                     /gene="cdd"
                     /locus_tag="PKM49_06300"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HNY82460.1"
                     /translation="MVEKRIVTVENLIKKAIQVMKKSYSPYSHFAVGAALLSKSGKVY
                     VGTNVENASYGLSICAERSAVVSAVSAGDRAFDCIVVATNTNPPSSPCGACRQFLSEF
                     GDFPVILVNPQGDRMDTTVDGLLPYSFRLNRSE"
     gene            complement(3191..4495)
                     /locus_tag="PKM49_06305"
     CDS             complement(3191..4495)
                     /locus_tag="PKM49_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNY82461.1"
                     /translation="MEPEPLSYSSLAFEILLLFVLLGFSAFFSSSETALSSMSKLKLK
                     SMLDQTDNAEKKKVIHTALHKPNQLLTTILVLNNFVNIFASSLSTLIVLRWLPPGNAS
                     TAAAISTGVMTFVILIFGEITPKIFSTQRAERIFHRCISFISFASYVLSPLVFLLVAI
                     SNFFIRILGGKKVQEPPFITEDDILSVMSVGEKEGAIELEERKMLTGALEMKETTVRE
                     IMVPRVDMVVLEENKTLQDAIKTIQEEGFSRFPVFRDNVDNIIGVVYAKDILKTLHER
                     GYEVLPKMKVKEIMHPPFFIPETKKINALLQDFKLNLVHLAVIVDEYGGTEGLVTIED
                     IIEQVMGEIMDEFDEGENTGVRKTSDFEYIIDSKVPINDIERELDIDFPDTEFETLGG
                     YLLERFERVPKVGEEMQVNGFHFKILAASRSKIEKIRFKIIK"
     gene            4895..6121
                     /locus_tag="PKM49_06310"
     CDS             4895..6121
                     /locus_tag="PKM49_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNY82462.1"
                     /translation="MYFPLVVLLFVYVLIAVRRIGSIRIKPWKVMLGGAVAVLITGQI
                     SPMEAVRAVNYDVIVFLFGMFIVGEAFGASGYMNHFSFRFFSKAKTYDQLLLYIIFSM
                     GLLSPFFINDTMVIVATPMLIILGRKLDLSPKPLLLAMAFAVTTGSVFSPIGNPQNLL
                     VALNGGIQNPFVTFFRYLTIPTLLNLFAVYVFIKAIYRKKGVNLRMESVSEEIKDAHL
                     AKLSAISFYLTFLLIILKVVTVLFGFGDQFRLTYIAILACLPIVLLSPKRWQILRHID
                     WRTLVFFPSMFILMQSVWNTGVFQEMVRHFDAGAIPTILLLSAGTSQFISNVPFVSLY
                     LPLLLLLNAETKALTALAAGSTIAGNLMILGAASNIIILQSAEKKGEIISFWDFSKVG
                     IPLTTFQLFFYWCFLR"
     gene            6465..7286
                     /locus_tag="PKM49_06315"
     CDS             6465..7286
                     /locus_tag="PKM49_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNY82463.1"
                     /translation="MKKKLFVLFCILLTGITFSLTRNGVELVYSYENLAPTNLVGEQI
                     TPDYAQVVRVVDGDTIKVKLPTGTETVRLIGVDTPETVHPTKAVEAYGQEASEFLKSL
                     CETDQDVYLSYDWDHRDVYKRLLGYLWIIGDDGEWVLANLAIVENGFGYAYLQYTFRK
                     DYMEIFAKAQEKAQEKGYGLWSETPAPSAGTVKQGDSKSTVEPDPQKGRYIGNVKSKV
                     LHDLDCGSLPAESNRIYFQTYVEAIKAGYKPCSKCKPSILKYWVQELASIFDFLF"
BASE COUNT         2123 a         1983 c         2042 g         2137 t
ORIGIN      
        1 tcaaaagatc aaaaaacagg gttcgcacag aggtcacaga gaacacggag gaaaacaggc
       61 aagggaccgc gttctccctg aaagagaaaa gacctgccga actaaagttc ggcgttccca
      121 ttttggtgta ccgcgatgtt atacccacaa aacggttgac ctaccttctt tagtgtgcta
      181 taataccctc gctttttagt tcgctggggc cgtagctcaa ttgggagagc gctaccttcg
      241 caaggtagaa gccgtgggtt cgaatcccat cggctccacc aagatgccga gatggcggaa
      301 ccggcagacg caccggtctc aaaaaccggc gaggttgatc cctcatgagg gttcgagtcc
      361 ctctctcggc accaaatacc cgactaggtc gggttttttc ttttcgttgc gtggttcttt
      421 cgaaatgtgt tatacttcga gcaaggagtg gtaaggggcg atttgctcgt cttttttgaa
      481 ctttattcct ttttttccag tcttataaac gagggggttc gaggttgctt tcccgaaaaa
      541 aacggggtaa gaggccgtta gacacagaaa gaaacggcga caaccttttt gtttgcgctc
      601 ttagagaaaa gcgaacttgg gcttacgaga agctctacga cgagtatgcc ggcttaatcg
      661 ggtccatcgc gaagtcctat ttgaacgtag atgatgtgga cgatgtcatc caagaagtgt
      721 tcttacgggt ctataaaagc attaagcaat ttaaaggtga ctccactttt tctacttggc
      781 tttacaggat cgcggtgaac gtatgcaagg atcaattgaa aaagtacgca aaacgatccg
      841 agacgatgac cgatttcgat tcagaagaac cgggccagct cgagcctgta gcagaccata
      901 atgtagagat cgcggtagag aaacaattcg attctgaaga tatggaggcc atcctcaaac
      961 ggctatcggc ggaagatcaa gagctgattc gactgagaga cgtacaggat ttggaatatg
     1021 aagagatagc gagtatcgtc gaaaaacctc ttgggaccgt aaaaagcaag atccattatg
     1081 ctcgaaaacg actgaaaaag ttattgacaa cctactatgg attgcgggaa gaagagaatc
     1141 agggaggaga ataatgaatt gtaagctcgt tcgtgaagag tatatggacc ggtatctcga
     1201 taaggagttg acattcgaag agatcaaaga actcaaagcg catttggaag gttgtcctca
     1261 gtgtcaaaag gccttttcca cctataaaac cttcatacaa atgcagaaag cgcggatatc
     1321 cttctctccc agcgctcacg gcaagcaaaa gctagtcaaa agaatacgac agaaaaagct
     1381 gctgccttac caattggccg gttccgttgc cgtcgccttc ttatgctttt tcgccgtgac
     1441 agccttcatc gaatcccgga tcatcaacca aaggtttgag aaaatcctaa gcactacgat
     1501 tcattcgatc actcccactt ccaacgcttc gatgacgatt tcgggaactc ctcgaaatga
     1561 caagaagtcc atctcaccgc aagattacgt agatcccgaa agaatactat acatcgtcaa
     1621 tgatggtgac taggtttcaa aaagccgcac atcctctggg atgccgtatg gggaagggta
     1681 gatctcttct cctgttcggc attttttttt attttttaac gcttacctcg tcgctttatg
     1741 gatttcagat caccggcgcg acctctccgg actcatcggg gaatgcgcat gagtacatcg
     1801 cgggcctatt gaaagcctac gagtcccaaa gtgttcaggg cacccgtatt tttttagaaa
     1861 gcggttcaaa aaaccggttt cgctatgaat acttcatacg gtctgcagac caaaagatcg
     1921 tgaccttgaa gatgcccttt gaagtaacct ggtttcgctt cgaagacgag ggctacctcg
     1981 tgcaaaacgg aacgaagacg aaggtggatg ttcctttgaa agacctcgaa gaccttctga
     2041 tcgaaaaatt gaagagctgt ccgatcacat tcagcgtttc agaggttctt tatacgggac
     2101 tgccggcctc ttttgtcgtg atcaacctgc cagaccacag tcaataccgc ttgatcctaa
     2161 tgcgcgacag ttaccaattc atcaagatcg aatatgagaa ggcggattct ctttcgatca
     2221 tgctctatga gcatctcttt ccgtatgaga gagaagtctt ttcggaagat atgaacttct
     2281 acaggagttt acccttttgg gatgatggca cgaaaaccac tgacgcgagc atcgcaaact
     2341 cggatggctt aggagaagga aatgctgggc ctctttccgc gcacggcgcg ctctactacg
     2401 attttgaaag gttcatcaca catctggtaa ggttttacac ccttgctaac atcgaggcgc
     2461 aagaacatcc ggggtactcc gtcctgatgg tcaaactgga attagagggc gatgcggcgc
     2521 cggttgccct tctattgttt cgatccaaag atccccgaaa cccttcccga atccctgaag
     2581 ccctccttgg caccatacca tccgattacg agatagccca cacggaagtc ggccgatata
     2641 cgatctatgt ctttggagaa gcagaaaagg cgcgtttgga atccattctt gacggacttg
     2701 ccgggcaggc cgccgaagaa gactagctgt ctattcggac cgattcagcc ggaaagaata
     2761 gggcaacaat ccgtctaccg ttgtgtccat acggtctcct tgcgggttta ccaggatcac
     2821 cgggaaatca ccaaactcgc tcaagaattg gcgacaagcc ccgcatggag aagagggcgg
     2881 gtttgtgttg gtcgccacga ctatacagtc gaaggccctg tctcctgcgc tcacggcgct
     2941 aacgacggcg gaccgctcgg cgcatatcga gaggccataa gacgcgtttt caacgttcgt
     3001 accgacatac acctttccag attttgagag caaagccgcg cccaccgcaa aatgagagta
     3061 gggggaatag ctcttcttca tcacttgaat cgcttttttt atcaaatttt cgaccgtaac
     3121 gattcgtttt tccacaatcc atcctttctt cacccgttta gaaagaggta ttctggacgg
     3181 gcgactagat ctatttgatt attttgaacc ttattttttc gattttactt ctgctcgcgg
     3241 cgagaatctt gaaatggaag ccgttcacct gcatctcctc tccgaccttc ggaacccgtt
     3301 caaacctctc cagaagataa cctccgaggg tttcaaactc cgtgtctgga aagtcgatgt
     3361 cgagttcgcg ctcgatatcg ttgatcggca cctttgagtc tatgatgtat tcaaaatcgg
     3421 aggtctttct aaccccggtg ttttcgcctt cgtcgaactc gtccataatc tcgcccatga
     3481 cctgttcgat gatatcctcg atggtcacca aaccttcggt gcccccgtac tcatctacga
     3541 tgacggccag gtggaccaaa tttagtttga aatcctggag caaggcgttg atctttttcg
     3601 tttccgggat aaagaatggc ggatgcataa tctccttgac cttcatcttg ggcaggacct
     3661 catatcctct ctcgtgaagc gttttaagga tatctttcgc gtatacaacg ccgatgatat
     3721 tgtccacgtt gtccctgaac acgggaaacc gtgagaaacc ctcctcttgt atcgttttta
     3781 tcgcatcttg gagcgtttta ttctcttcga gaacgaccat atcgacacgc gggaccatga
     3841 tctccctgac cgttgtttct ttcatttcga gcgcgccggt tagcattttt ctttcttcca
     3901 gttcgatcgc tccttccttt tccccgacac tcataactga gagaatgtca tcttctgtga
     3961 tgaacggggg ttcttgaacc ttcttgccgc cgaggatacg gataaaaaaa ttgctgatcg
     4021 cgactaaaag aaagacaagg ggtgaaagta cgtaactggc gaaagaaatg aacgaaatgc
     4081 agcgatgaaa gatccgttcg gcgcgctgcg tcgaaaaaat cttcggggta atctcaccga
     4141 agatgaggat gacgaaggtc atcacgccgg ttgaaatcgc agccgccgtg ctcgcgtttc
     4201 ctggaggaag ccagcgaagg acgatcagtg tagaaagcga cgaggcgaaa atgttgacga
     4261 aattgttgag gaccagaatg gtcgtgagca actgattcgg tttatgcaaa gccgtatgga
     4321 tcactttctt cttctctgca ttatccgttt ggtccagcat actcttgagc ttcaatttgc
     4381 tcatcgaaga taacgcggtc tcagaactgg agaaaaaagc ggaaaaacca agtaaaacga
     4441 acaggagcag gatctcaaaa gctaacgagc tataactcag aggttcaggt tccacgaaaa
     4501 aaaacacctt ccttatgtat tgatgatgag acggagatcc ccagacgtat cttctataaa
     4561 atccctatga taggagacca gggctctcaa tagaatagac cggttctcat caaggtaaaa
     4621 ggccgactcg cgcaaaccga tcttaggggt gaagtataga gcgttaccat atggatcggc
     4681 gcaaacgcct ttcgtatagc ccacacagtc gcttcccgcg tcctaagcgc gaaaaagccg
     4741 agaggatcag accagattat aacatacaaa acaggagaaa gaaatagaat cgctttccct
     4801 ttcccttttc cttatttatg ttagaatagc cgcgattatc ggcgaaggcg ttcgggagag
     4861 ggtgtcggag atggtaaacg cctttttttc caagatatat ttcccgcttg tcgttttgct
     4921 tttcgtctac gttctcattg cggtgcgacg gatcgggtct atacgcatta aaccctggaa
     4981 ggtgatgctt ggcggagcgg tggcggtctt gatcacggga cagatatcgc ctatggaggc
     5041 ggtaagggcg gtcaactacg acgtgatcgt ctttcttttc gggatgttca tcgtcgggga
     5101 ggcctttggc gccagcgggt atatgaacca tttttccttc cggtttttca gcaaagctaa
     5161 aacctatgat caattattgc tctatatcat attttcgatg ggtttactct ctccgttttt
     5221 tatcaacgac acgatggtga tcgtcgcgac tcctatgctt ataatcttgg gaagaaaact
     5281 ggacctttcc ccgaagcccc tcctactcgc gatggccttc gcggtgacca cgggaagcgt
     5341 cttcagcccg atcgggaacc cgcaaaacct attggtggcc ttgaatgggg ggattcagaa
     5401 cccattcgtc acgttctttc gctatctgac gattccgacc ctgctcaacc tcttcgccgt
     5461 ctacgtcttc atcaaagcta tctacaggaa gaaaggggta aatttgcgga tggaaagcgt
     5521 ttccgaagag atcaaagatg cgcatttggc gaagctatct gcgatatctt tctatctgac
     5581 gttcctttta atcatcctta aagtcgttac cgtactcttc gggtttggag accagtttcg
     5641 gttgacctac atcgccattc tggcctgcct acctatcgta ttattgagtc cgaaacgctg
     5701 gcagattttg cggcacatcg attggcgaac cctcgttttt ttcccttcga tgtttatcct
     5761 gatgcaaagc gtgtggaaca ccggggtctt tcaagagatg gtcaggcatt tcgatgcggg
     5821 ggcgattccg acgatcctcc tcctcagcgc gggaaccagc caattcatct ccaacgtgcc
     5881 cttcgtatcg ctctatttac ccctgctcct cctgttaaac gctgaaacga aagcgctgac
     5941 cgcgttagcc gcggggagca cgatagcggg aaatttgatg atcttaggag cggcgagcaa
     6001 cattatcatc ctacaaagcg cggaaaaaaa gggagaaatc atctcatttt gggatttttc
     6061 gaaagtcggg atccccttaa caacctttca actcttcttc tactggtgtt ttctccgata
     6121 ggaacgtccc tccgggaact tccggaaacg ccggacttta gtccggcatg tctgtgtttc
     6181 ttgggagcgc atcgggaact tctgggagcg ccggacttta gtccggcatg ttttggtttc
     6241 tcgagagcgc atcgggaact tctgggaacg ccgaacatcg gttcggaatg gctttttggc
     6301 gcgccctgaa ggcgttcacg ggaatcattc ctccgtgtcc tccgtaggct ccgtgagaaa
     6361 ccttttagtc cttttttaga ctacatatcc cgaggggcgc ccatcaagcg cgactcctat
     6421 atcgtgttat aatcaatagt ggaattaccg ttagggggta gctaatgaaa aagaagctct
     6481 ttgttctatt ttgtattctt ttaaccggca taacctttag tttgacgaga aatggtgtag
     6541 agctcgtcta ttcctatgaa aacttggcgc ccacgaattt agttggcgag caaataacgc
     6601 cggactatgc gcaagtcgtg agggtggttg acggagatac catcaaggtg aaattaccca
     6661 caggaacgga aacggtccgg ttgatcgggg tggacacacc ggagaccgta caccccacga
     6721 aagcggttga ggcctatgga caagaggcct ccgagttcct aaaaagccta tgcgaaactg
     6781 accaagacgt ctatctgagc tacgattggg atcacagaga tgtgtacaaa aggctattgg
     6841 ggtatctctg gataataggc gacgacggag agtgggtatt ggcgaacctc gccattgtcg
     6901 aaaacggatt tgggtatgcc tatctacaat atactttccg taaggattat atggagattt
     6961 ttgctaaggc ccaggaaaaa gcgcaagaaa agggatatgg gctttggagc gaaactcccg
     7021 ctccctcagc gggtacggtg aagcagggag actccaaaag taccgttgag cctgatcctc
     7081 aaaagggccg ttatatagga aacgtcaaat cgaaagtgct ccacgatttg gattgcggtt
     7141 ccctacctgc cgagagtaat aggatatatt tccaaaccta cgtagaggcg atcaaagcgg
     7201 gttataaacc ctgcagcaag tgcaaacctt cgatactcaa gtattgggtc caggaactcg
     7261 cctccatttt tgattttcta ttttgaacgc ctacacgaaa gtttcgctag aagcgccgaa
     7321 cttttatttt actcggttct ttgggaaccc ttgcgaacgc cgtactttag cgcggtacgg
     7381 gttcctgaga ggaaaaaacg gggttcgcac agagaacatg gagggcacaa aggaaagatt
     7441 agaacacatc tgagagcgcc tgggaacgcc aaactttagt ttggcatggg ttcttgagaa
     7501 gaaaaaaagg gttcgcacag agaacacgga gaacacaaag gaaagattag aacacatctg
     7561 agaccgcctg ggaacgttga acttaagttt gggatggttt cctgagagga aaaaaatggg
     7621 gttcgcacag agaacacaga ggacgcaaaa gagagattag aacacatctg agagctctgg
     7681 gaacgttccc aagcgcagca tcgcggcgta agcgctcttt tttctcgcga cggcgcgata
     7741 cggtttttaa agaaagaagg cggagcacac gtttctgtgg aatagaaacg ccgcactttt
     7801 gtgcggcgtg ggttcattgt tctctctgtg tcctccgttc gaaacctttt ttcaagacct
     7861 gccaacttaa aagttggcgt tcccagggcg tttctctgtg ttctctccgt gctctctgtg
     7921 tactctgtgc gaaactatct tttggtcttg aaaagacctg ccaacttaaa agttggcgct
     7981 cccaggcttt cccagggcgt ttctctgtgt tttctccgtg ctctctgcgt gctctgtgtg
     8041 aaactatctt ttggttttga aaagacctgc caacttgaaa gttggcgttc ccaggctttc
     8101 ccagggcgtt tctcagtgtt ctctccgtgc cctctgcgta ctctgtgcga aactatcttt
     8161 tggttttgaa aagacctgcc aacttgaaag ttggcgttcc caatagggct tcactgagac
     8221 ctattgatga tccctacgca gacggtacgg accgtttgac cgggatgatc cagaggtgaa
     8281 gggtc
//
LOCUS       DAOPXN010000093         8203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8727_length_8203_cov_6.179062, whole genome shotgun sequence.
ACCESSION   DAOPXN010000093 DAOPXN010000000
VERSION     DAOPXN010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8727_length_8203_cov_6.179062"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1080..1766)
                     /locus_tag="PKM49_06320"
     CDS             complement(1080..1766)
                     /locus_tag="PKM49_06320"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNY82464.1"
                     /translation="MEERKPCGAHEERSGMPLLGDKFPNMTVQTTRGEMVLPEAFKGK
                     WFVLFSHPADFTPVCTTEFVAFQMRYDKFRALNTELIGMSVDQVFSHIKWEEWIHEKM
                     GVEIQFPIIADTGAVANKLGIIHPGKGTNTVRAVFVVDANGIIRIILYYPQELGRNMD
                     EILRVVEAMQFADKNKVAMPANWPKNEVFGDKVIIPPAKDVKTAKERLEQAKTGAFEC
                     KDWWLCYKKI"
     gene            2114..2758
                     /locus_tag="PKM49_06325"
     CDS             2114..2758
                     /locus_tag="PKM49_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNY82465.1"
                     /translation="MNRNFCSAQLLAACHRIETSGMTVGTWGNISIRVDTTHFLITPS
                     GMKYSLLEGRDMVLMDMDGRVVESDRKPSIENALHRGIYRARPDVQAVLHTHPKYSIA
                     FAIARKDIPPVCEELVQIIGEGVRCAAYALPGTEELAKNAVEALGDRNAALIANHGAV
                     CVGESIDKAFLVAEVLEKSAQGILYATLLGTPHILSHEDCVAMRDFVTHSYGQR"
     gene            complement(2886..5069)
                     /locus_tag="PKM49_06330"
     CDS             complement(2886..5069)
                     /locus_tag="PKM49_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HNY82466.1"
                     /translation="MSSDTQQTAHKQSFFVKGMTCVTCVKHVERAIKKVDGISFVSVN
                     LATESGFALSNQEIPFDELKKAVSNAGYEITDSVAPDAMQKRFQEAGRNLILSWAVTI
                     PLMVMMFLHMSGRHVPAFEYVEPLIAALVIFYTGRHTIKGAWIALIHWHFNMDTLIFL
                     GSVTAFATTLLALVGLKIASFGSIGAMIVAIHLTGRYIESWLRDRAAKDVKALMQLQA
                     TEARVKSEDGWVMVPIEAVKLDSLVRVYPSERIPLDGEVVEGASSVDESMITGEYMPL
                     QKQTGSPVTGGSLNLNAPITIRVTGTGEDLFLSQLISMVQDAQGTKVPIQALADRITL
                     WFVPAVLLLAISAGLIWFFRYDALRPMLTSFEKAIPWVNANADALSHSLFVFVATLVI
                     ACPCALGLAIPMALIGASSLSAKKGLIIRNGEAIQTAGEIGAVILDKTGTLTVGKPSV
                     VESSVPDELLPAIAQLETQSAHPLAKSLVEYAKEKGLHRQVVIDEVLETAGSGIGGKW
                     EGKDLFVGKPTSLEPYRLWAEKGYSIVEARVNGDPLGAFAITDPIREDTVEGIAALKA
                     FGLKIVLATGDHEEAAKRVCAETGIRDYRSQMKPSEKLTLIHESRHGKEKVLMVGDGL
                     NDGPALKAADIGIAMGSGTDLAIDSADIVIVRGGIPQVVESIRISRMTFRIIKQNLLW
                     AFLYNVIAIPAAMLGVLHPAIGEIAMAFSSITVILNSLRLKYHRD"
     gene            complement(5778..7433)
                     /locus_tag="PKM49_06335"
     CDS             complement(5778..7433)
                     /locus_tag="PKM49_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82467.1"
                     /translation="MLRIPKKLNFALEEQVGPPELFVGRQKEFSWFLGGWYDNLIRGK
                     PKSQAIVARRKKGKTVFLQRLFNVHWTAHNPEVIPFYFTIPEIRTTQAEFAKLFLRAL
                     INQLVAFEMNEPQLVTKPVAFEEIPAITQDPVYLEYHRALLSYEKISNWNMMWDSASR
                     APSEIASLRDLRIIQIIDEFQYINEYVVDQGGKVIDTMSGTYLTLAEQRKAPLIVSGS
                     EVHWLLRIVNGLVGRFDSWSMENWPEDEAKQAVIQYAKHYQTRVDKEAIEHIWKLTQG
                     DPLYISQLFSSRHNEKKNFTDPLVITEVYEKEMKQGGGIYGTWMEYMAKIFKDVNESN
                     AKRIILYLFQQARECTRAEIIQDLKLKESEQQVQEKLQALIKADLISQGSGFYRYTIS
                     RDKTYEWVFRGAFQEEIDHFVPDIRSEALSQMGIRNYEIGLFGEFKLRRKLQKGFYLN
                     EIAKNGPQIWVDPHPIQERVPKKIENQTFEIDLIVESDPEIWIEVKTLKKKFGKTQLR
                     RILQLHQALSKTAKAPILFVYAPGGFTKEASEELQKHGILYGM"
     gene            7828..>8203
                     /locus_tag="PKM49_06340"
     CDS             7828..>8203
                     /locus_tag="PKM49_06340"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82468.1"
                     /translation="MKKIDKPHDKLFKEVMGDIETAKSFIQHYLPPKIVRLIDPESIT
                     IETDSYIEKDLSEYFFDLLFRVKMQQEEAYVYLLFEHKSRVDKHVNLQLLGYMTSIWK
                     KTVPRPLPVILPVVFYQGREKWE"
BASE COUNT         1828 a         2158 c         2111 g         2106 t
ORIGIN      
        1 gcggatcgcg agacttcgtg ggcttccgaa gtcagcccgc tggttacccc gcgatgaccc
       61 gcacgctaat cgcggcgtaa gcgccccttc atttcgcgac ggcgcgatac cccggttttc
      121 gttcgtttcc aagataaaaa acagagttcg cacagagagc acagagggca cggaggagtg
      181 acggaggggt gtaaaaaact taaaaacatg ccggactgaa gtccggcgtt cccagggcgt
      241 tctcaggtgt tcttgcgtgg cggcatcgcg acgtaagagc cccttctttt cgcgacggcg
      301 cgataccccg gttttcgttc gtttccaaga taaaaaacag ggttcacaca gagggcacag
      361 agagcacgga ggagagatta ggaccaattc agaattgatt atggaaggcc agcccgggaa
      421 cgctggactt tagttcggaa ttgtttacct cgaaataata aaaatgacaa aaggcggatc
      481 gcgggtcttg aaaggctggg aacgccaact tttaagttgg catgtctttt ttgaagagaa
      541 aaggaggatt gcgcacagtg ggcacggaga aaacatacat cgcggagtaa gcgtcccttc
      601 ttttggcgac ggcgcgatac gtttttgaat tttcatctag tttctgatac cttatttacg
      661 tatagagaga aagaagggat cgcgcagtcc tgttagttac tgcgcgataa cccacaagaa
      721 agcggattgc ggggtagtaa aaaccactta cccagcgata gcgcacacgg gcatcgcagt
      781 gaaaacacat aagaattctc gggagcttcc agaaaagaaa aaacatgccg aactaaagtt
      841 cggcgttccc agagcgggtc acaagaacaa aaagagtatt gcgagacttc gtgagccgtt
      901 ttgttttctc ctcctctttc cttttcttgt tgcgcttaga tattataccc tcacgtatcg
      961 ctctattcgt gggcgggaca atggtgcaaa ggcaaagttt aacgcgccat acggtcgact
     1021 cagttttgct aataaaaaaa ggcgcaaacc acgcgccttt ttcgcgaccg gggcggacgc
     1081 taaatttttt tgtagcaaag ccaccagtct ttacactcga atgctccggt tttggcctgc
     1141 tccaaacgct ctttcgcggt ttttacgtct ttcgcgggag gaatgatcac tttgtcgccg
     1201 aacacttcgt tcttcggcca gttcgccggc atcgccactt tgttcttgtc cgcgaactgc
     1261 atcgcttcca cgacccggag tatttcgtcc atattcctgc cgagctcctg gggatagtac
     1321 aggatgatcc ggatgatccc gttcgcgtct acgacgaaga cggccctgac ggtgttggtc
     1381 cctttgccag ggtggatgat ccccaacttg ttcgcaacgg cgccggtatc cgcgataatc
     1441 gggaactgga tttcgacgcc catcttttcg tgaatccact cttcccattt gatgtgagaa
     1501 aacacttggt cgacgctcat accgatgagc tcggtgttca atgccctgaa tttgtcgtat
     1561 ctcatttgga acgcgacgaa ttcggtggtg cagaccggcg taaagtcagc cgggtggctg
     1621 aacagaacga accatttgcc tttgaaagcc tccggtaaca ccatctctcc tcgcgtggtc
     1681 tgtacggtca tattcgggaa cttgtccccc agtaacggca ttccgcttcg ctcttcgtga
     1741 gcgccgcacg gttttctttc ttccatcgaa aactcctcct tcaatttgaa atttgcagct
     1801 cattacatgg acgaaaaaag aaagattctt attcatttgg attttttctt tgtagtttta
     1861 cacattaata ctctacaatt aaaaactatt gttcagggat tgctttcctt tccggcgact
     1921 tgatttttct ggccatccct tctataattg gagttggcag ggatcaagcc aaagaaaggg
     1981 ctccgtcacc tctaattttc gcaaatctgt gacctttcag ccttcgaagg cagaaaatga
     2041 cgcgagaaac tggagaaaac caacgacagg accgggtcta gaaaagccgg aggggcccat
     2101 tggaggaagg cagatgaatc gtaatttttg cagcgcacaa ctattggccg cttgtcaccg
     2161 tatagaaacc agcggtatga cagtcggaac ttggggaaac atcagcatac gtgtggacac
     2221 aacccatttt ttaatcactc cgagtgggat gaaatacagc cttctggagg gtcgcgatat
     2281 ggtgttaatg gatatggacg gccgggtcgt cgaaagcgat cgcaaaccca gcatcgaaaa
     2341 tgcgcttcat cgggggatct accgcgcccg accggatgtt caagcggtcc ttcacacgca
     2401 tccgaagtat tcgattgcct tcgcaatcgc gcggaaagat atccctccgg tctgtgaaga
     2461 gctggttcaa attatcggag agggtgtgcg ctgcgcggcc tacgccttgc ccggtactga
     2521 ggagttggcg aagaacgcgg ttgaggcgtt aggagaccgg aacgctgcgc ttattgccaa
     2581 tcacggggcc gtatgcgtcg gggagtcgat cgataaggcc ttcttggtag cggaggtctt
     2641 agaaaaatcc gcccaaggca tcctctatgc gacactgctc ggtacgcccc atatcctctc
     2701 ccacgaggat tgtgtcgcta tgcgggactt cgtcacccat tcctacgggc aaaggtagaa
     2761 aaaagaagaa aacgcgtccg gctatgggtc gctaggcgcg tccccgtacg gttggagggt
     2821 cctcgcttcg tttacacctc gtttaaacga tcggggggga agccggggct cccaccgctt
     2881 ggtgcctaat cccgatgata ctttaatcgg agcgagttta aaatgacggt gatcgagctg
     2941 aaagccatcg cgatctctcc gatggccggg tgcaggaccc cgagcatagc cgcgggtatg
     3001 gcgatcacgt tgtacaagaa ggcccacagc aggttctgct tgatgatccg gaaggtcatc
     3061 cgcgagatac ggatgctttc gaccacttgc gggatgccgc ctcggacgat gacgatatcg
     3121 gcgctgtcta tcgccagatc cgttcccgag cccatcgcaa taccgatatc cgcggccttc
     3181 aaagcgggac cgtcgttgag tccatctccg accatcaaga ccttctcttt gccgtgacgg
     3241 ctttcgtgga tcaacgtcag cttttccgaa ggcttcattt ggctcctata atccctaatc
     3301 ccggtctccg cgcacacccg tttggccgct tcctcgtgat cccccgtcgc caggacgatc
     3361 ttgaggccaa aggctttgag ggcggcgatc ccttcgactg tgtcttctcg tatggggtcg
     3421 gtgatggcaa aggctcccag agggtcgccg ttgacccgcg cttcgacgat agaataccct
     3481 ttttccgccc ataggcgata aggctccaga ctcgtcggct ttcccacgaa caggtctttg
     3541 ccttcccact ttccaccgat accggagccg gcggtttcca gtacttcgtc tatcacgact
     3601 tgccggtgga gtcccttttc tttcgcatat tccacgagcg atttggcgag agggtgggcc
     3661 gattgcgttt cgagctgcgc gatcgcgggt aacagttcgt ctgggaccga cgattcgacc
     3721 accgagggtt tgcctaccgt cagggttccg gttttatcga gtatgacggc gccgatctct
     3781 cccgccgttt gtatcgcctc tccgtttcgg atgatcagcc ctttctttgc ggaaagggag
     3841 gaggcgccga tcaaggccat cgggatcgct agccctaacg cgcaggggca cgcgatcacg
     3901 agtgtggcga cgaagacgaa gagactatgc gagagggcgt cggcgttcgc gttgacccaa
     3961 gggatcgctt tttcaaaaga ggtcaacatc ggccttaaag cgtcgtagcg gaaaaaccag
     4021 atcaacccgg ccgaaatcgc caggaggagc acggccggga cgaaccacaa ggtaatccga
     4081 tccgcaagcg cttggatcgg tacttttgtg ccctgggcgt cttgtaccat cgagatcaac
     4141 tgggataaga aaaggtcctc tccggttccg gtcaccctga tcgttatggg ggcgttgagg
     4201 ttgagagagc ctccggtgac cggcgatccg gtttgttttt gcaggggcat atactccccg
     4261 gtgatcatcg actcatcgac ggaagaggcc ccttcgacca cctctccgtc caacggaata
     4321 cgctcggagg gatagacgcg caccaggcta tcgagcttga ccgcctcgat cggaaccatc
     4381 acccacccgt cttccgattt cactcgcgcc tccgtcgctt gtaactgcat cagcgctttg
     4441 acatctttcg cggctcgatc gcgcaaccaa gattcgatgt accttcccgt gaggtggatg
     4501 gccacgatca tcgcgccgat acttccgaag gaggcgatct tcaaccccac gagcgccaga
     4561 agcgtcgtgg cgaacgcggt gaccgatccc aaaaagatca gcgtgtccat attgaaatgc
     4621 caatggatca aggcaatcca cgcgcccttg atcgtgtggc gtccggtata gaagatcact
     4681 aaggccgcta tcagcggttc aacgtactcg aatgcaggca cgtggcgtcc ggacatatga
     4741 aggaacatca taaccatcag cggaatggtt accgcccaag ataggatcaa gtttcgaccg
     4801 gcttcttgaa accgtttttg catcgcgtcc ggggcgacag agtctgtgat ttcgtatccg
     4861 gcgttcgata cggccttttt caattcatcg aaagggattt cttggttgga taaggcaaac
     4921 ccggattccg tcgcgaggtt gacggaaacg aaagaaatgc cgtcgacctt ctttatcgcg
     4981 cgctccacgt gtttgacaca ggtcacgcag gtcattcctt tcacaaagaa actctgtttg
     5041 tgcgccgtct gttgcgtatc gctcgacata tccgttctcc tttctctctt ttgcgtgtca
     5101 catttttctg ggagcgccct gggaacgccg aactttagtt cggcatgcct ttctgtgaac
     5161 agtagctttc aagttgactt gctttctgtt ctctgggaac gccctgggaa cgccgaactt
     5221 tagttcggca tgcctttctg ggaacggcag ctttcaagtt gacttgcttt ctgctctctg
     5281 ggaacgccct gggaacgccg aactttagtt cggcatgcct ttctggaaac ggcagctttc
     5341 aagttgactt gctttctgtt ctctgggaac gccctgggaa cgccgaactt tagttcggca
     5401 tgttttctag cttttcaaaa atcaaacgca tccttactca aagcccccgc cctctgctag
     5461 ccgccccttt acttcttaga cgagacaaaa ccgtaaaaga ttcagagcct gaaaacccgt
     5521 gccagttctg caaatcgaaa atctttgctc gttcgtgagg tcgattcctg ctcggattga
     5581 gcttggtgct gcctttttga gaccatcctt ctttttgctg gatgttctta ggaaaataga
     5641 taccgtgcca acttaaaagt tggcgttccc agggcgttcc aagtgttccc aggcgttccc
     5701 agcagttcga cgctccctgg gaacatcctc gttgccttct gtgtgagact tttttctcca
     5761 gcagggcgcc cgcacgctta catcccgtag aggataccgt gcttttggag ttcttccgag
     5821 gcttccttag tgaagccgcc tggcgcgtag acaaaaagaa taggagcctt tgccgttttg
     5881 gaaagcgctt gatggagttg tagtatccgc cggagctggg tctttccgaa tttctttttc
     5941 aacgttttga cttcaatcca aatctccggg tcgctttcga cgatgaggtc gatctcgaag
     6001 gtttggttct cgatcttctt cggtacgcgt tcttgaatgg ggtgaggatc gacccatatc
     6061 tgagggccat ttttagcgat ttcgttcaag tagaagcctt tttgaagctt tcggcggagt
     6121 ttgaactcgc caaacagccc gatttcgtag ttccggatcc ccatttgact aagagcttcg
     6181 cttcgtatat ctggaacaaa atggtcgatc tcttcctgga atgcaccgcg aaatacccat
     6241 tcgtaagttt tatcgcgact gatggtatag cgatagaaac cgctcccctg actgatgaga
     6301 tccgctttta tcaaagcctg aagcttttct tgaacctgtt gttcggattc ctttagcttc
     6361 aagtcttgga tgatctccgc acgcgtgcat tcccttgctt gctggaaaag gtatagaata
     6421 atgcgtttag cgtttgactc gttcacgtct ttgaagatct tcgccatata ctccatccag
     6481 gttccgtaga tgccccctcc ctgcttcatc tccttctcgt acacttctgt gatgacgagc
     6541 gggtcggtga agtttttctt ttcgttgtgc cggctggaaa ataattgtga gatatacagc
     6601 ggatctccct gtgtgagctt ccagatgtgt tcgatggctt ctttgtcaac gcgcgtctga
     6661 tagtgtttgg cgtattgtat taccgcttgt ttggcttcat cttccggcca gttttccata
     6721 ctccagctat cgaaccgacc aaccagacca ttcacgatgc gcagcaacca gtgcacctcg
     6781 cttcccgaga cgatcaatgg cgcttttctt tgctccgcca gggtaaggta tgtgccactc
     6841 atcgtgtcga ttaccttacc tccttgatcc acaacgtatt cgttgatgta ttggaattca
     6901 tcgataatct ggataatccg caagtctctg aggctggcta tttcggacgg cgctctggat
     6961 gcgctgtccc acatcatatt ccagttggaa atcttctcgt acgagagcag cgcgcgatga
     7021 tactccaggt acacagggtc ttgcgtaatg gccggtatct cctcgaacgc taccggtttc
     7081 gtaacaagct gcggctcgtt catttcaaag gctaccagtt ggttgatcag cgcgcgtagg
     7141 aagagtttgg cgaactccgc ttgcgtcgtt cgaatctcag gtatagtaaa gtagaacggg
     7201 atcacttccg ggttatgagc agtccaatga acattgaaca gccgctgtag gaaaacggtc
     7261 ttcccttttt ttctgcgcgc gacgatcgcc tgactctttg gctttcctct gatgaggttg
     7321 tcgtaccacc cgcccagaaa ccaggagaat tctttctggc ggccgacgaa aagttcgggt
     7381 ggacctacct gctcttccaa agcgaaattc agcttctttg gaatccttag catctacaca
     7441 ccctcctcca tatcttcgtt acacctcatt atagcacagt gttctaagct gcgaatctgg
     7501 cgagtgtggg tcaagttagc ggtgctattt accccgcgag gatcctcgtt gtcatcgcag
     7561 cgttagcgct tcttctttgc gcgacgacgc gatacgcttt ttttcgggcc tttctcggct
     7621 aaaaaagcaa aggcggaatg cggagacctg ttggttaccc cgcgataacc ttcgttgtca
     7681 tcgcggcgta agcgcttttt cttttcgcga cggcgcgata caacgtattc gtcgcacaaa
     7741 aaaagacgaa aggctgatgc actttccctt tttgaaatcc cgcctttacg gtataatacc
     7801 gatgaaactt ttttggaggg gatatctatg aagaaaatag ataagccgca cgacaaattg
     7861 tttaaagaag ttatgggaga tatcgaaacg gcgaagtcct tcatccagca ctatctgcct
     7921 ccgaagatcg tccggttaat cgatcccgaa tcgataacga tagaaacaga cagttacatc
     7981 gagaaagacc tctcggaata ctttttcgat ctgctctttc gggtgaagat gcaacaggaa
     8041 gaggcgtatg tctatctgct gttcgagcat aaaagcaggg ttgacaaaca cgtgaacctt
     8101 caactcctgg ggtatatgac ctccatatgg aagaagaccg taccgcgacc tctgccagtg
     8161 attctgcccg tggtcttcta ccaagggaga gagaaatggg aag
//
LOCUS       DAOPXN010000094         8203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8728_length_8203_cov_5.239445, whole genome shotgun sequence.
ACCESSION   DAOPXN010000094 DAOPXN010000000
VERSION     DAOPXN010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8728_length_8203_cov_5.239445"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1124
                     /gene="trpS"
                     /locus_tag="PKM49_06345"
     CDS             132..1124
                     /gene="trpS"
                     /locus_tag="PKM49_06345"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNY82469.1"
                     /translation="MRILSGSRPTGHLHIGNYVGALQNWVKLQNEGHETCYFVADWHV
                     LTTAYDKTEELAELSKELVKILVAVGLDPDKSILFVQSAIKEHAELCLLFSMITSVQR
                     LERIPTYKEAKEQVKGTTDINTSGFLLYPVLQAADILMYLADGVPVGEDQLSHIELTR
                     EIARRFNFIYNCELFPEPASLLSKFPKLLGSDGRKMSKSYGNVILIDNNEKQLTEQIL
                     PMITDPARKRKTDPGNPEVCPVWDYHKAFGVTPEQSALIVDGCTHAKIGCIECKRILI
                     KNMVAIFAPIWEKIRLIDQSPGYIEQIIFDGNDKARRIAKSTMEKVRAAVNLLF"
     gene            1140..1838
                     /locus_tag="PKM49_06350"
     CDS             1140..1838
                     /locus_tag="PKM49_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNY82470.1"
                     /translation="MCPEEFTFKIEQFEGSLDLLLFLVKKREMDIRDIQIAVIADDFI
                     AYLDRLKSLDFSVTSDFLVMASSLMELKSKTLLAQDEGNIEEAESARKKLVHQIEEYE
                     RLKDFTAFLEMKEEEAAKTFPTRAKKENAQRLLNREIPNRLFDVFKQAYQELKLREKV
                     YRVKGEKYSLSKRVEQLLLWLSEGQTLWLEELFTQAEDRIDLIVTFLAALEIIKLEKG
                     IYSEESGAAVLKKA"
     gene            2026..3675
                     /locus_tag="PKM49_06355"
     CDS             2026..3675
                     /locus_tag="PKM49_06355"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNY82471.1"
                     /translation="MAENPNPKPRYPIQTIIDKLGIKAPCYHYGDTIAKIPYTLSEQR
                     PAKRGKLVLVTAITPTAAGEGKTTTVIGLGQAMNRLNKKAIVTLREPSLGPVFGLKGG
                     ATGGGLSQVLPMEEINLHFTGDIHAVTTAHNLLSAMIDAHIHFNNETELLPGSIYWPR
                     VMDMNDRSLRQIIVGLGGSGNGIPREDSFRITVASEVMAILCLSKSIQDLKERLGNIT
                     IGRNKDKRFVKAKELAAEGAMAALLKHALNPNIVQTTDGSPAIIHGGPFANIAHGTNS
                     IVATNTSLNFGEYALVESGFAADLGGEKFLDFVTKVGDFWPDTVVLVASVRALKLHGG
                     ASKKELDQEDLPAVQKGIENLKVHLTNLRKFRVPVVVALNHFYKDHPSEINWVMEECA
                     KMGAEIALSDVYGKGAEGGLDLARKVVDLCERTPNPQRIYYWNEPIKTKIERVAKEIY
                     HAGKINYTLDAEKKIRVFSKEFESLPIIIAKTQYSISDDPEKLNAPSGYEFTVKDLSL
                     STGAGFIVVYAGDIMVMPGLPAVPAATKIDVNENGEITGLF"
     gene            4054..4142
                     /locus_tag="PKM49_06360"
     tRNA            4054..4142
                     /locus_tag="PKM49_06360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4088..4090,aa:Ser,seq:gga)
     gene            complement(4188..5906)
                     /locus_tag="PKM49_06365"
     CDS             complement(4188..5906)
                     /locus_tag="PKM49_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HNY82472.1"
                     /translation="MIGALYSGLSGLLNFQKAIDVTGNNSANIDTIAYKHSRVTFKTA
                     VEYMLTGSTNAKIGTGTMLNTIDRNMVQGPIENTGRYSDLAIAGSGFFAAGDFRRNAN
                     GELVKMNSYLTRVGKFEVLSNLNLVQGDVGLHILGIPSTIVNGEITKGEIPDQEAMIR
                     MTPQEKAAFYDSLKLIDLVKYQTMGGKATGAFEVFGTMASEVGPQPHVFELTSTTDSR
                     VSYKVRLSFDRDVSDLSTVFQDDKTYHYKLEVTDWSQGGYDRPPIVGSSGTITFDKAG
                     NIASINGGNGQLLTFEINGETFKLSRAEVLAACDYKDPSVLVIDTFYNSSGEIVKDGV
                     VFEKLSKGTWLFRPARYDAKVASVSFIGESGAEYSFEDEELNLLGGVLRFDENGRYEG
                     LSLMKGDGALISDTPTHMKWSLLDGTEQSMAINFHNILSGGGVQNDLQLLQEGGRATG
                     SLRGLEFDKSGYLIGTYSNQKTATLAVLPLVQMRVPETLTISSLYSTAYNFDPIIQAN
                     NFTGVFEAGNGGTGLIEPYALEYSNVETAKEMTRLIIYQRALQLNARSVTTADAILQQ
                     AYELKR"
     gene            complement(5950..6744)
                     /gene="flgG"
                     /locus_tag="PKM49_06370"
     CDS             complement(5950..6744)
                     /gene="flgG"
                     /locus_tag="PKM49_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNY82473.1"
                     /translation="MITALYSGATGMTGQQHKLDTISNNLANTDTTGFKRVRTEFVDL
                     FYQYAQNAGVPVLAQTSTLPSGVYTGLGSRVAATSRIFTEGNIEETGSQLDVAISGDG
                     FMQVQLQDGTTAYTRDGAFKLDNEGVLVLNTGYRIINETIIVPTEAVAIQFASDGTVA
                     AVYADDTLEVLGQIQLVRFINPTGLDALGNNLYRATPASGEPQEGIANQDGFGSFVQG
                     YLEKSNVNAVREMVSMITSQRAYELNSRSIQTADDMLRTVYGLKRS"
     gene            complement(6810..7574)
                     /locus_tag="PKM49_06375"
     CDS             complement(6810..7574)
                     /locus_tag="PKM49_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HNY82474.1"
                     /translation="MVRGIYTAAMGMLVDQAIVDNASNNLANVDTTGFKRDVQAFRSY
                     LQHEILRIEAKDGRQKRTPLGTLERGVTLDEVRADHTQGAMEMTEVNTDFAIDGDGFF
                     AIQKPNGDVYYTRNGNAKINAQDILVNDQNDPFLNDVGDMIPFSFNTVVDPQGFILQD
                     GVIQGRLGLYSFESVRHLRKVGYTLYQPTTRSGEPQLDTESRIYQGYQEKATVNAINE
                     MVKLIAAQRHFDISQKVVTTEDQLLDAAVNKVGPTR"
     gene            complement(7768..>8203)
                     /locus_tag="PKM49_06380"
     CDS             complement(7768..>8203)
                     /locus_tag="PKM49_06380"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNY82475.1"
                     /translation="ELKKRYEIDIGERSLSMLERFFTPREETETILLNGKSKITGLPV
                     SIVVEKTEAEQYLNEYVEEIIQLIKSVIQPLSPEIISELFESKILLSGGGVSHRKLQE
                     AIPAVLKLEALSQGSPENIPLLGFSYLDQIGWSAVKPFSEKA"
BASE COUNT         1979 a         2168 c         2142 g         1914 t
ORIGIN      
        1 atgccgaact aaagttcggc gttcccaatt tggtgtaccc cgatattata cccactcaac
       61 tgcagggaaa tgagaacctc ctacggtgtg gtagaatcaa aacattaaac agacaagtaa
      121 ggaggagaat aatgagaata cttagtggga gtcgcccgac gggccatctt catatcggga
      181 attacgtcgg agcgctgcaa aattgggtaa aactgcagaa cgaaggacac gagacttgct
      241 attttgtcgc tgattggcac gtcctcacaa ccgcttacga taaaacggag gaactggcgg
      301 agctctctaa agagctcgta aaaattctcg tcgccgtggg attggatccg gataaatcta
      361 tccttttcgt gcagtccgcg ataaaggagc acgcggagct gtgcttactc ttttctatga
      421 tcacctccgt gcagcgatta gaaagaattc caacgtataa agaggccaag gagcaagtta
      481 aggggacgac cgatatcaat acctccggct ttctcttgta tcctgtcctc caagccgcgg
      541 atattttgat gtatctggcc gacggtgtgc ccgtaggcga ggaccagttg agccacatcg
      601 aactgacgcg agagatcgcg aggcggttta actttatcta taattgcgaa ctgttcccgg
      661 aaccggcctc cttgctttcc aagttcccaa agctgttggg gagcgacggg cgcaagatgt
      721 ccaaaagtta cggtaacgtc atcctcatcg acaacaacga aaaacagttg accgaacaga
      781 tcttgccgat gatcaccgat cccgccagaa agcgtaaaac cgacccgggg aatccggaag
      841 tctgtcccgt atgggattac cacaaagcct ttggagtcac gccggagcaa agcgcgctga
      901 tcgtcgacgg atgtacccac gcgaaaatcg gatgtatcga gtgtaaacgg atcttgatca
      961 aaaatatggt tgcgattttc gcgccgatat gggaaaagat ccggttgatc gaccaaagcc
     1021 cggggtacat cgaacagatc atctttgacg gaaacgataa ggcccgccgt atcgccaaat
     1081 cgacgatgga aaaagtccgg gccgccgtca acctactttt ctagtcacgt tccgcggcga
     1141 tgtgtccgga agaattcacc tttaagatcg agcagttcga ggggtcttta gacctcttgc
     1201 tctttctcgt gaaaaaacgc gagatggata tccgagacat acagatcgcg gtgatcgcgg
     1261 acgacttcat cgcctacctg gaccggctta aaagcttgga cttttccgtc acctcggact
     1321 tcctcgtgat ggcctcgtcg ctgatggagt taaaatctaa aacgctctta gcccaagacg
     1381 aagggaacat cgaagaggcg gagtccgcgc ggaagaagtt ggtccatcag atagaagagt
     1441 acgaacgatt gaaggacttc acggcctttt tggagatgaa agaggaagag gcggcgaaga
     1501 cctttccgac gagagcgaaa aaagagaacg cgcagcgctt gctgaaccgc gaaatcccaa
     1561 atcggttgtt cgatgtcttc aaacaggcct accaggagct caaactacgg gaaaaggtct
     1621 atcgtgtgaa aggagaaaag tactccctgt ccaaacgcgt cgagcagctt cttctttggc
     1681 tgtcggaagg ccaaacgcta tggctcgaag aactgttcac ccaagccgag gatcgaatcg
     1741 atctcatcgt cacgtttttg gccgcgttgg agatcatcaa gctggaaaaa gggatctact
     1801 cggaggagtc cggagcggcc gtcttaaaaa aggcttgacc ggcggattgc caggtaaaac
     1861 atcgctacca taccggcata ccgttcctta gaaaagtgcc cgaaaaagcg acgtaaagag
     1921 agaggcgcgg gtcgatcttg gtttttttgc gtgtggggat gttaaaatca cagtcaattt
     1981 atgctatact cagttgattt ctttgttttt taggagggac aacaaatggc cgaaaatcca
     2041 aatcccaagc cccgataccc catacaaacg atcattgaca agctagggat aaaggcgccg
     2101 tgttaccatt atggcgatac gatcgccaaa attccctata ccctttcaga acaacgccca
     2161 gcgaagagag gaaagctggt gctcgtcacc gcgatcaccc cgaccgccgc cggagaagga
     2221 aagaccacaa ccgtcatcgg tctggggcaa gctatgaacc gcctgaacaa aaaagcgatc
     2281 gtcacgttga gagaaccttc cttaggtccc gtgtttggct tgaaaggcgg ggcgacgggc
     2341 ggtggcctgt cgcaggtctt gccgatggaa gaaatcaacc tccatttcac cggtgatatc
     2401 cacgcggtga cgacggccca taacctgctt tctgccatga tcgacgcgca tattcacttc
     2461 aataacgaaa cggagttact ccccggctcg atctactggc ctcgagtgat ggatatgaac
     2521 gatcggtctc ttcgccagat catcgtaggg cttggaggtt ccgggaacgg cataccgaga
     2581 gaagactctt ttcggatcac tgtcgcctct gaagtgatgg ccatactctg cctgtcgaaa
     2641 agtatccagg acctgaaaga gcgcctcggg aacattacta tcgggcgaaa caaagacaaa
     2701 cgcttcgtga aggcgaaaga attggccgcg gagggcgcga tggcagcctt actgaaacat
     2761 gccctcaacc cgaacatcgt tcaaacgacc gatggttcgc cggcgatcat ccacgggggc
     2821 cccttcgcca acatcgcgca cggcaccaac tccatcgtgg ccaccaatac gtcgctcaac
     2881 tttggcgagt acgcgctcgt cgaatctgga ttcgccgccg atttgggcgg cgaaaagttt
     2941 ttggatttcg tcacgaaggt cggggacttc tggccggata ccgtcgttct cgtcgcctcg
     3001 gtgagagccc tgaaactaca cggaggcgcc tccaagaaag aactggatca agaagacttg
     3061 cctgccgtcc agaaaggaat cgagaacctc aaagtacacc tcacgaattt acggaaattc
     3121 cgtgttccgg tcgtcgttgc gctcaaccat ttctataaag accaccctag cgaaatcaac
     3181 tgggttatgg aagaatgcgc aaaaatgggc gcggagattg ccctgagcga tgtgtatggg
     3241 aaaggtgccg agggaggttt agacctggcg agaaaggtgg tcgacctctg cgaaagaact
     3301 cctaacccgc aacgcatcta ctactggaac gaaccgataa aaaccaagat agagagagtg
     3361 gccaaagaga tctatcacgc cggaaagatc aactacaccc tggatgcgga gaaaaagata
     3421 cgcgtctttt ctaaggagtt tgaatcttta ccaatcatca tcgccaaaac ccaatactcg
     3481 atctccgatg atccggaaaa actcaacgcc ccgtccggtt acgaattcac cgtcaaggac
     3541 ctttcgctct cgacgggcgc cggctttatc gtcgtttacg ccggagacat catggtgatg
     3601 ccgggactac ctgccgtacc cgctgcgaca aagattgacg tgaatgaaaa cggggaaatc
     3661 accgggttgt tttagtgttt tattaaaata acgagttcgc ccttcagtcg aacaccatcg
     3721 atgcaacggg ggacgccccg gttaccatca taaagcaagc gtttagcgaa tcggagcgga
     3781 acaggccgat acagctggcc gcttggtgtg ggtcaaattt tgaggtttaa ccaagaaacg
     3841 aggtcggcat cgatcgcacg atttttagtc aatgtagtca tcgcggcgta agcgcctttt
     3901 ctttccgcga cggcgcgata cgtatttgta cgtgtcttga aaaacagggt tcacacggag
     3961 ggcacagaga aaagattctt gggaacgccg atattatacc cgcccggtcg cttgaccgaa
     4021 agtgggagat gtgttataat tcacgcgcat tgcggagaga tggccgagtg gtctaaggcg
     4081 cacgcctgga gagcgtgtta cgctaacccc gtacgtgggt tcaaatccca ctctctccgc
     4141 caataaaaaa gcgcccttag gggcgctttt ttggtagcct cgcattatta gcgcttgagt
     4201 tcgtaggcct gctgcaagat cgcgtcggcg gtcgtaacgg agcgggcgtt gagctgtagc
     4261 gcccgttgat agatgatcaa gcgggtcatc tctttggcgg tttccacgtt cgaatactcc
     4321 aaggcgtaag gctcgatcag accggttccg ccatttccgg cttcaaagac accggtgaag
     4381 ttgttcgctt ggattatggg atcgaaattg taggccgtgc tgtacaggga agagatggtt
     4441 agggtttccg gtacgcgcat ctggacaagc ggtaagaccg cgagcgtagc agttttctga
     4501 ttcgagtagg tgccgatgag gtacccggat ttgtcgaact ccaaaccccg gagactcccg
     4561 gtggcccgcc caccttcttg gagtagctgc aaatcgtttt ggacgccacc ccccgatagg
     4621 atattgtgga aattgatcgc catagactgc tccgttccat ccagaagcga ccacttcata
     4681 tgcgtgggcg tatcggagat caacgcgccg tcgcctttca tcagcgaaag gccttcgtac
     4741 cgtccattct catcgaaccg gaggacgccc cccaacaaat tcagctcctc gtcctcgaaa
     4801 gagtattcgg cgccgctttc tcctatgaaa gaaacactcg cgaccttcgc gtcgtatctg
     4861 gccggccgga agagccaagt ccctttcgag agcttttcga aaacgacgcc atccttaacg
     4921 atttcgcctg agctgttata gaaggtgtcg atcaccagaa ccgagggatc tttgtaatcg
     4981 caagcggcca acacttcggc cctcgacagc ttgaaggtct caccgtttat ctcgaaggtt
     5041 aagagctgtc cgttccctcc gttaatggag gcgatatttc cggctttatc gaaagtgatc
     5101 gtcccgctgc ttcccacaat cggtgggcga tcatatccac cctgagacca gtcggtcact
     5161 tccagcttgt aatgatacgt cttgtcatcc tggaagaccg tggagagatc gctgacgtct
     5221 cggtcgaagg agagacgtac cttgtaggat acacgagaat cggtggtgga ggtcagttcg
     5281 aacacgtgag gttgcggtcc cacttccgac gccatcgttc caaagacttc gaacgcgccg
     5341 gtcgctttcc cgcccatcgt ttggtatttt actaaatcga tcagctttaa gctatcgtaa
     5401 aaggccgcct tctcttgggg cgtcatccgg atcatcgctt cctggtcggg aatctcgcct
     5461 ttcgtaatct ctccattaac gatcgtagaa ggaattccca atatgtgtag gccaacgtcc
     5521 ccttgaacga ggttgaggtt cgaaagcact tcgaattttc ctacccgggt gagatagctg
     5581 ttcatcttaa cgagctcccc gttggcgttc ctacggaagt ccccggctgc gaagaacccg
     5641 ctccccgcaa tagccaaatc gctataccgt ccggtgtttt cgatgggccc ttgcaccata
     5701 ttccggtcga tggtgttcaa catcgtgccg gtcccaattt tggcgttagt ggaccccgtc
     5761 agcatatatt cgacagcggt tttgaaggtc acgcgcgaat gcttgtacgc gatggtatcg
     5821 atattggcag agttattccc ggtcacgtcg atcgctttct gaaagttgag caatccgctt
     5881 aacccgctgt agagcgctcc gatcattttc tctcacccct tatatgagca ttaagcgcta
     5941 aaacctacct taactccttt tcaacccgta aacggttctc aacatatcgt ctgcggtttg
     6001 tatagagcga gagttgagtt cgtaggcccg ttgggatgtg atcatgctga ccatctcccg
     6061 gacggcgttc acgttcgatt tttccaaata gccttgaacg aaagagccga acccatcctg
     6121 gttggcgata ccctcctgag gttcaccgga agcgggagtc gcgcggtaca ggttgtttcc
     6181 gagcgcatcg agcccggtcg ggttgatgaa tcggacgagc tgtatctgac ccaacacctc
     6241 cagcgtatca tcggcataga cggccgccac cgtcccatcg gacgcgaatt gtatggccac
     6301 ggcctctgtc gggacgataa tcgtttcgtt tatgatccga tatcccgtgt tcaggaccag
     6361 cacgccttcg ttatcgagct tgaaggcgcc gtcgcgagtg tacgccgtcg tgccgtcctg
     6421 aagctgaacc tgcatgaacc cgtcgcccga aatcgctaca tcgagctggc tgccggtctc
     6481 ttcgatattc ccttcggtga agatacggct tgtggccgcc acccgagaac ccaaaccggt
     6541 gtaaacgccc gaaggcagcg ttgaggtttg ggccaaaacg gggactccgg cgttttgcgc
     6601 gtattggtag aacagatcga cgaactccgt ccgcacccgc ttgaatccgg tcgtatcggt
     6661 gttcgccagg ttgttggaga tcgtatcgag tttatgctgt tggccggtca ttcctgtggc
     6721 gccgctgtat aacgctgtga tcatgatctc acctcaatgt atggaaaata ctcttcccgt
     6781 cctatttttc actacgattt cttaaactgc taacgcgtcg gacccacctt gtttaccgcc
     6841 gcgtccaaca actggtcctc cgtcgtgaca accttctgcg agatgtcgaa atgccgttgt
     6901 gcggcgatca atttcaccat ctcattgatc gcgttgacgg tagctttttc ttggtatcct
     6961 tgataaatac gactctctgt gtctagctgc ggttctccgg aacgcgttgt gggctgatac
     7021 agggtgtatc cgactttgcg gaggtgtcgg accgactcga aagagtaaag gcctaacctc
     7081 ccctggatca ccccatcctg caggatgaaa ccctggggat cgacgacggt attgaaagag
     7141 aaggggatca tgtcgcctac atcgttcaag aagggatcgt tttggtcatt gaccaaaata
     7201 tcctgcgcgt tgattttggc gtttccgttc cgggtatagt agacatcgcc gttcggtttc
     7261 tggattgcga aaaacccatc tccatcgatc gcgaaatcgg tgttgacctc tgtcatttcc
     7321 atcgcgcctt gggtatggtc cgcgcggact tcatccaacg tcaccccgcg ctcgagcgta
     7381 cctaaaggcg tccgtttttg tcgaccgtct ttggcctcta ttctcaggat ttcatgctga
     7441 agataggatc ggaacgcttg aacgtcccgt ttgaaaccgg tggtgtccac gttggcaaga
     7501 ttattggagg cgttatccac gatcgcctga tccacaagca ttcccatagc tgcggtataa
     7561 attcctctga ccacgttctc cctcccggct tctgcgcgac aacacgaata acgggaacaa
     7621 cccgtaggcg cccaccgatt catgtttcgc caaaagtaat agaaaatcct aaccggctgg
     7681 aattcgcgtc gctgccagct gactcaccct caagacccgg actgagcgcg gcagtgcagt
     7741 cggggggcgg tgtcgtagtg ggcgagatta agccttttct gaaaagggct tcacggcaga
     7801 ccaaccgatc tggtcgaggt aagaaaagcc aagcaaagga atgttttccg gactgccctg
     7861 agacagagcc tccagtttta gaacggccgg tatcgcttcc tgcagtttcc ggtgagaaac
     7921 cccgccaccg ctcaatagga tcttactttc gaagagttcg gagatgatct ccggagagag
     7981 aggctgaatc actgacttaa tcagttgtat gatctcctcc acgtactcgt tgaggtattg
     8041 ttcggcctcg gttttttcca ccacgattga aacgggaagt cctgttattt tactcttccc
     8101 gtttaataaa atcgtttcgg tctcttcacg gggggtgaaa aaccgctcta acatcgacaa
     8161 agagcgttcc ccgatatcga tctcataccg tttcttcaac tcg
//
LOCUS       DAOPXN010000095         8171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_8777_length_8171_cov_5.210079, whole genome shotgun sequence.
ACCESSION   DAOPXN010000095 DAOPXN010000000
VERSION     DAOPXN010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8777_length_8171_cov_5.210079"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..689)
                     /gene="raiA"
                     /locus_tag="PKM49_06385"
     CDS             complement(135..689)
                     /gene="raiA"
                     /locus_tag="PKM49_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNY82476.1"
                     /translation="MDNRLITRGFSPSDSIQEYVEKKISKLDKTLPEGDHVKVDMRLQ
                     KEREVFSAEITVLIRGGTLRVQEKTADVYASIDKAVEAMESKLKKYKEKKQLRHKAGV
                     IGIGEEYAKLTPPVFEKNEKEKEFEIVRTKRFFLKPMSVEEAILQMDMLDHDFFVFKN
                     EETDETNLVYKRKNGRIGLIEFEG"
     gene            complement(706..1737)
                     /locus_tag="PKM49_06390"
     CDS             complement(706..1737)
                     /locus_tag="PKM49_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82477.1"
                     /translation="MEKETFALGLPLHKRYAILGLEKNIGKTTVLNWISRVWGRNSQS
                     TLLLSIGRDGEEVDVIDGHAKPKIELQKGNYALTGERVLTSPALLEVVEVFSFKTLAG
                     RPVLVKAIEDTTIELINPGGLERLTEIADQLLAQGRCDRVLLDGAFNRLSHAQRAVAD
                     SVLLVTGAQAQGTFEEILEKTVYVVERLERLPSSVEWRTRIEEADRPDAPMILLSEEG
                     KTMVVGETSWQNPKTIAEMRKARAVYMKGALTPGIADKLLQERVKTEVILRDGPCIQL
                     DAGLYRRMQAGGLRLSVLYPIPVIGIFVNSFGAKRSFTNGRMKGELARRLPANPVFDL
                     FEVTETANK"
     gene            complement(1713..2984)
                     /gene="ablA"
                     /locus_tag="PKM49_06395"
     CDS             complement(1713..2984)
                     /gene="ablA"
                     /locus_tag="PKM49_06395"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HNY82478.1"
                     /translation="MRDYRSISLWKGVTPEQWNSWTWQIQNRIMDVATLEKVLPLSES
                     EKKAIAHSLDFLRMAITPYYATLINPQDPLDPVRIQAVPTLKELEVQKEDMLDPLHED
                     VDSPVPGLTHRYPDRVLLLLTDMCSMYCRHCTRRRKAGETDNALPIAHVDEAVEYLKE
                     HTEVRDVLLSGGDPFTLSTDYLEKIIRKIKAVPSVEIIRIGSRVPAVLPQRIDDTLVS
                     MLKKYHPIWLNTHFNHPNEITPASERALALLADNGVPLGNQSVLLRGVNDHPEIMKKL
                     VHLLVKNRVRPYYIYQCDLSKGLYHFRTTVAKGLEIMEYLRGHTSGLAVPTYVIDAPY
                     GGGKIPVMPQYLLSIGENKVILRNYEGGLFVYDEPRDYHSSADPDALLEQNRKSTNGV
                     ALMLSGEKKSLVPSETTRLHRVEEWKKKHSR"
     gene            complement(3015..3497)
                     /gene="coaD"
                     /locus_tag="PKM49_06400"
     CDS             complement(3015..3497)
                     /gene="coaD"
                     /locus_tag="PKM49_06400"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNY82479.1"
                     /translation="MSVAVYPGTFDPITYGHIDVAERAAKVFSDLIILVMNNPGKSNA
                     MFSVEERVSLIQNVFSNISNICVASYRGLLVDYARLTGHNVIIRGLRAVSDFEYEMTM
                     AAANRRMYDSFETFFLMTDTKYSFISSSLVKEIAINGGKIDPWVPESVKQEIYKKLEK
                     "
     gene            complement(3516..5771)
                     /locus_tag="PKM49_06405"
     CDS             complement(3516..5771)
                     /locus_tag="PKM49_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HNY82480.1"
                     /translation="MKRLIGGVFLVLVLLFSLTNCTVISLYTLEGRVSLIGESVPEAV
                     VSVWSLSNPEKRKETLTDNDGRFRFDGISVGEWGIRAEYAEPDYLRFTYEATFAAQAG
                     LFQTLALDVVPFEERPWNLTVLSFSDPMTEAELAQYFEPFHYFELTFLQKSDAGRLES
                     FYLSEGEIRPFYRPVTPNFSEGAKKALKVAMEAFPAEKSVLYVIDDGHGWRTGARGPY
                     PEDVFYDKTTSILEFDAMLEDLRMDVLLSTGPYGGLLENLYQVRDHFKTAVAFEGLSP
                     TTYPELGGLFSRLRSLPPPDALAFAEAACAFFCDAAASLEEDLIAAQSGMGCSVIDLS
                     ELEGLGSSLKKVFDQLTLLLTEASLKEAVKKDLGFTLDWNTLAFDYPDYKDVRSWLTV
                     LKHCIDGALYAGDYRVKSREDGYVYDLQEPMLQQLFEYIDEAEDRVDRAVLLNQHYGF
                     CEDLIENGKKVGKKNTAQMGGLSFWYPLSVKGGLWNVYGKDKYDRLSFSQKTGWTTFL
                     NRLMSVDQLPAKQLLLLDREGDVVARDFQPGKPLESSGATINFGFLDREAIDRFWMVF
                     GEEHLTEQIIEERGGLCFEDESSIRCYLPVRVTDAGKRVGARILLRGKEEPVNFDYGS
                     ILPVLKGKSLVVTDQHGNGLLQVSPGNELYISTKHYVVIESEVEEWATPLALTAEVRL
                     EGQTETVTYALAEAHRYDGMVLFRSGMIPASVFGSSPSSRTFFTLKQMQTKNQTENQT
                     ETENWIIHLVP"
     gene            complement(5811..6890)
                     /locus_tag="PKM49_06410"
     CDS             complement(5811..6890)
                     /locus_tag="PKM49_06410"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR01208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HNY82481.1"
                     /translation="MGFTKAVVLCGGEGTRLKPFTFSRPKQLLPLANRVLIAYVIDSI
                     KSCGIDEVCLVVNHHNRKAFEQTLKDGGGFGIRITYVLQEHPKGIAHALSLCENWAAG
                     EKILLYLGDNLLEENLETMKSLFLNTQAQATILLKEVEDPRAFGVATLQNGRIVRLVE
                     KPKEPESHNAVLGLYFFDEQVFDVIRRLKPSWRGEYEITDAIAMLIAEGHVVNYHFLK
                     GWWKDTGKVLDLLDANRRILLHKPAEKAGQIELEHTFLGEDVSVGENCAFTNCRIEGP
                     TIIGNNARFQNVYIGPYSAIGNDCALEHIELENSILMDRVQLSNLPVRVKESILGCDF
                     EWSATYPDAGELRIIAGDHNKIIFS"
     gene            complement(6911..7942)
                     /locus_tag="PKM49_06415"
     CDS             complement(6911..7942)
                     /locus_tag="PKM49_06415"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNY82482.1"
                     /translation="MAIIRVLICDDSAFMRMIIKDILDGQGDIEIVGQARDGMEAVEK
                     AVQLKPDVITMDVEMPKLNGLEALKLISRKIQTRVIMVSSLTQEGANITIEALQNGAI
                     DFVPKPSGSISTNFRIMGNTLLAKIREAASIDPSKIQYRPLPIAKRTSRSFTAKGKIV
                     LIASSTGGPKSLDQVIPALPKNLPAPVVLVQHMPAGFTASLAQRLNRISEIEVKEAQS
                     GDVLKQGTVFVAPGDYHVGLRAESGDIRVFLDQSPKINGVRPAADFTFEHAAELYKDK
                     TIGVVMTGMGRDGTKGSFKIKHYKGIVLAESEETCVVYGMPKSVVQEGYSDYVLPSYR
                     LAEKISELV"
     gene            complement(7914..>8171)
                     /locus_tag="PKM49_06420"
     CDS             complement(7914..>8171)
                     /locus_tag="PKM49_06420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNY82483.1"
                     /translation="SLVESLLDLFYPFFHEEVKKAKDLAAGIVLVTAVFAVSVGLIIF
                     GKHLFHLPDLIGLFAFFLFIVTLLLLIGKGMTHGDHSRTHL"
BASE COUNT         1862 a         2184 c         1965 g         2160 t
ORIGIN      
        1 ttgttttttc ttattttttc cctttttctc caaaaccggt cgaaagtcgg gctagaaaac
       61 gcctactcac tacaatttag tttcgaagaa taaaaaaacc ggcgttcgcc ggttttcttc
      121 tttcttttat atgattatcc ttcgaattcg atcagaccaa tgcggccgtt cttccgttta
      181 tacacgaggt tggtttcatc ggtctcttcg ttcttgaaaa cgaagaaatc gtggtctagc
      241 atatccatct gcaagatcgc ttcttccacg ctcatcggtt tgaggaagaa gcgcttcgtg
      301 cgcacgatct cgaattcttt ctccttttca ttcttctcga aaacgggtgg ggtcaacttc
      361 gcgtattctt ctccaatgcc gatgacgcca gccttgtgcc gcagttgttt tttctctttg
      421 tactttttca acttcgattc catggcttct accgccttat cgatagaggc gtagacgtcc
      481 gcggtttttt cttggactcg tagggttcct ccgcggatta acacggtgat ttcggcggag
      541 aagacttctc tttccttttg taggcgcatg tccactttta cgtggtcgcc ttccggtagc
      601 gttttgtcca attttgagat ttttttctcc acgtactctt gtatcgagtc actggggcta
      661 aaacctctcg tgatgagccg attatccata ctaccactcc tttcttcatt tatttgcggt
      721 ttctgtgacc tcgaaaagat cgaagaccgg atttgctggt aaacggcgcg cgagctctcc
      781 tttcatccgg ccgttggtga agcttctctt cgcgccaaaa ctattgacga aaatcccgat
      841 gacgggtatg ggatacaata ctgatagacg taatccgccc gcttgcattc gtctgtaaag
      901 gccggcatcc aattggatgc agggaccgtc ccgcaagatg acttcggttt ttactctctc
      961 ctgaaggagt ttgtccgcga tccccggggt gagcgcgcct ttcatataga cggctctcgc
     1021 ttttctcatc tcggcgatgg tcttcgggtt ttgccacgat gtttctccga cgaccatcgt
     1081 ttttccctct tctgataaca gaatcatagg agcgtccggt cggtcggcct cttctatccg
     1141 cgtccgccat tccacggagc tggggagcct ttccaaccgt tcgaccacgt agacggtttt
     1201 ctcgaggatc tcttcaaatg tcccctgagc ctgagccccg gtcacgagca agacggagtc
     1261 cgcgacggcc ctttgggcgt ggctgagccg gttgaacgca ccgtccaaca gcactcggtc
     1321 acaccggcct tgagccagaa gctgatcagc gatctcggtc aggcgttcca aaccgcccgg
     1381 gttaatcaat tctatcgtcg tgtcttcgat ggccttgacg aggaccggtc ttccggcgag
     1441 ggtcttaaaa gagaagactt cgaccacttc caaaagcgcg ggcgaggtga ggacgcgttc
     1501 accggtgagg gcgtagttcc ccttctggag ttcgatcttg ggcttggcgt gaccatcgat
     1561 gacgtccacc tcttctccgt ctcgaccgat gctcaacagg agagtagatt gggagtttcg
     1621 cccccatact cgcgaaatcc agttgagtac cgttgttttg ccgatatttt tctctaggcc
     1681 taagatcgca tagcgtttgt gaagaggaag gcctaacgcg aatgtttctt tttccactct
     1741 tcaacgcggt gcaagcgcgt cgtttcggat ggaaccaagc tcttcttttc tccgctcagc
     1801 atcaacgcca ctccgttcgt gcttttgcga ttctgttcca ataaggcgtc cggatccgcg
     1861 gaggaatggt agtctctcgg ttcgtcatag acgaacaagc cgccttcgta gtttcgtaag
     1921 atgaccttgt tctccccaat agacaacaga tactggggca taacgggtat tttccctcct
     1981 ccgtagggcg cgtcgatgac gtaggtgggg acggccaaac cggaggtgtg tcctcgtaaa
     2041 tactccataa tctccaaacc tttcgccacc gtcgtgcgga aatggtacaa tcccttggag
     2101 aggtcgcatt ggtagatgta gtacggacga acgcggtttt tcaccaggag gtggaccaac
     2161 ttcttcataa tctcgggatg gtcgttgact ccacgtaaga gaaccgattg gttgcctaag
     2221 ggtacgccat tgtcggctag tagcgccaac gctcgttcag atgcgggagt gatttcgttc
     2281 ggatggttga aatgcgtgtt caaccagatg ggatgatact tcttcagcat cgagacgagc
     2341 gtatcgtcta tccgctgagg taggacggcc gggacgcgag atccgattcg gatgatttca
     2401 actgatggca cagccttgat ttttcggata attttctcca aatagtcagt gctcaaggtg
     2461 aagggatctc ctccagacag aaggacatct cgtacctcgg tgtgctcctt aaggtattct
     2521 acggcctcat ccacgtgggc tatcggcaag gcgttgtcag tttctcccgc ttttcgtcgt
     2581 cgcgtgcaat gccggcaata catggaacac atatccgtaa gaaggagtag gacacggtcc
     2641 gggtatcggt gcgttagccc cgggaccggg gaatcgacgt cttcgtgcaa aggatcgagc
     2701 atatcctctt tttgcacttc gagttctttc agcgtgggca ccgcttggat acgaacggga
     2761 tccagggggt cttgtgggtt gattaatgtg gcgtaatagg gtgttatcgc cattcttaaa
     2821 aagtctagac tgtgggcaat cgcctttttc tctgactcgg ataacggcaa caccttctcc
     2881 aacgtcgcga catccataat ccggttttgt atttgccagg tccaactgtt ccattgttcc
     2941 ggggtgaccc ctttccatag gctgatagat cgatagtctc tcacaattgg aaacctccca
     3001 tcgtatagtc ttttctattt ctccaacttt ttataaatct cttgcttaac tgattcggga
     3061 acccatgggt cgatcttccc gccattaatc gcgatttcct tcactaagct cgaagagata
     3121 aaagaatact tggtgtcggt cattaaaaag aaagtttcaa acgaatcgta catacgtcga
     3181 ttcgcggcgg ccatcgtcat ttcatactca aagtccgaca ccgccctcaa cccgcgtatg
     3241 atgacgttgt gtccggtcaa gcgggcgtag tcgaccaaca agcctcgata actagcgacg
     3301 cagatattag agatgtttga gaacacgttt tgtatcaacg agacgcgttc ctccacacta
     3361 aacatggcgt ttgatttccc gggattgttc atcactagga tgatcaagtc cgaaaatacc
     3421 tttgccgccc gttccgccac atcgatatgg ccataggtga tcgggtcaaa agttcccgga
     3481 tacaccgcta cagacatgtt ctctcctttc gagaatcaag gtaccagatg aatgatccaa
     3541 ttctccgttt cagtttgatt ttcggtctgg tttttcgtct gcatctgttt cagagtgaag
     3601 aaggtgcgag aggatgggga ggagccgaat accgaggcgg ggatcatacc ggaccggaac
     3661 aaaaccatcc catcgtaacg atgcgcctca gccaaggcat aggttacggt ttctgtttgg
     3721 ccttctaagc gaacttcggc cgttagcgcc aagggcgttg cccactcttc cacttccgat
     3781 tcgatcacca catagtgttt cgtagagata tagagttcgt tgcccggcga tacttgcagt
     3841 agaccatttc cgtgttgatc cgtaacgacc aggctttttc ccttcagcac cggaaggatc
     3901 gacccgtaat cgaagttgac cggttcttcc ttgccgcgaa ggagaatgcg cgcgccgacc
     3961 cgtttaccgg cgtccgtgac tcgtaccggg agataacagc ggatagaact ctcatcctca
     4021 aaacacaggc cgcccctctc ctctatgatt tgctcggtca agtgctcttc tccaaacacc
     4081 atccaaaacc tgtcgatcgc ctcacgatcg agaaagccga aattgatcgt cgcaccggaa
     4141 gattccaaag gctttcccgg ttgaaagtca cgtgcgacaa cgtcgccttc gcggtccaaa
     4201 aggagcaact gtttggcggg cagctgatcg acgctcatca atcggtttaa aaaggtcgtc
     4261 catccggtct tttgcgaaaa cgacaaccga tcgtacttat cttttccgta aacgttccac
     4321 aggccccctt tcaccgaaag tggataccaa aagctcaatc cgcccatctg agccgtattc
     4381 ttttttccca ctttctttcc gttttcgatt aagtcttcac aaaatccgta atgttgattc
     4441 aaaaggacag cccggtccac ccgatcttcc gcctcgtcga tgtactcgaa aagttgttgc
     4501 aacatcggct cttgcagatc atagacgtat ccgtcctctc tgctttttac cctatagtcg
     4561 ccggcgtaga gcgcaccgtc tatgcagtgt ttcagaacgg tcaaccagga acgcacgtcc
     4621 ttatagtcgg gataatcgaa agctagggtg ttccaatcca acgtgaagcc gagatctttt
     4681 tttaccgctt ctttcaaaga ggcctctgtc aagagaaggg tgagttgatc gaagaccttt
     4741 ttgagcgaac tgccgaggcc ttcgagctcg ctcaaatcga tcacggagca tcccattccg
     4801 ctctgcgccg cgatcaagtc ttcttccaaa gaagccgcgg cgtcgcagaa gaaagcgcaa
     4861 gcggcttccg cgaaagcgag cgcatccggc gggggaaggc tccttaagcg cgaaaaaagg
     4921 cctcccagtt ccggataggt agtcgggctc agaccttcga aagcaacagc tgttttaaaa
     4981 tgatccctca cttggtagag gttttctaac aaaccgccgt atgggcctgt agataggagc
     5041 acatccatcc tcaagtcttc gagcatcgcg tcgaattcca agatagacgt cgttttgtca
     5101 tagaaaacat cttcgggata ggggccccga gcaccggtcc gccatccgtg tccgtcatcg
     5161 atcacgtata acacggattt ttccgccgga aatgcctcca tagccacttt tagtgccttt
     5221 ttggctcctt cggaaaagtt tggcgttacg ggacggtaga aaggacgaat ctcaccttcc
     5281 gaaaggtaga agctctccaa acgccctgcg tcactttttt gcaagaaggt caactcaaaa
     5341 taatggaaag gttcgaagta ctgggccagt tccgcttccg tcatcggatc cgaaaaagat
     5401 aggaccgtca ggttccacgg tctctcttcg aacgggacga cgtcaagcgc taaggtttga
     5461 aatagtccgg cctgggccgc aaaggtggct tcataggtga agcgcaggta gtcaggttcc
     5521 gcgtattcgg cgcgaattcc ccattcgcct acgctaatcc catcaaagcg aaagcgcccg
     5581 tcgttgtctg ttagcgtctc tttacgcttt tccgggttcg aaagactcca gacagaaacc
     5641 acggcctccg gcaccgattc gccgattagg ctgacccgtc cttccaaggt gtaaagagag
     5701 atgaccgtgc aattggttaa ggaaaaaagc aataccagga ccaagaaaac gccaccgatc
     5761 agtcttttca tgtttacccc ccacttcgta tacacataca aacaatagga ttaagaaaat
     5821 atgattttgt tgtgatctcc ggctatgatt ctcaattcgc cagcatctgg ataggtagca
     5881 ctccattcga aatcgcaccc gagaatgctc tctttcactc tcacaggaag gttactcaac
     5941 tggaccctgt ccattagtat actgttctct agctcgatgt gctcgagagc gcaatcattc
     6001 ccgatggctg aatacggccc gatgtagacg ttttgaaaac gggcgttatt tccgatgatc
     6061 gtcgggcctt cgatgcgaca attcgtgaag gcgcagttct cgccgaccga aacgtcttct
     6121 cccaaaaaag tgtgctcaag ctctatctgc cctgcttttt ccgccggttt gtgcaaaagg
     6181 atacgacggt ttgcgtccaa taaatccaaa acctttcctg tgtccttcca ccatcccttt
     6241 aagaaatgat aattcacaac gtggccttcg gctatcagca tcgcgatggc gtccgttatc
     6301 tcgtattcgc cacgccagga gggttttaac cgccgtatca catcgaaaac ctgttcgtcg
     6361 aaaaagtaca aaccaagcac ggcattgtga ctttcgggtt ccttgggctt ctcgacgagc
     6421 cggacgatcc gcccgttttg aagcgtcgcc accccgaaag cccgggggtc ttccacctct
     6481 ttcagtaaga tagtcgcctg cgcttgcgtg ttcaaaaaca gggacttcat cgtttccaga
     6541 ttctcttcca gcaggttgtc gcctaaatac aaaagaatct tctcgccggc cgcccaattt
     6601 tcacataggc tcaaggcgtg ggcgatccct ttcggatgct cttgtagcac gtaagtgatc
     6661 cgtatgccga acccgccgcc gtcttttaac gtttgctcga aggctttccg attatgatgg
     6721 ttgacgacca aacacacctc atcgataccg caactcttga tcgaatcgat gacgtaggcg
     6781 attaggaccc gattagcgag cgggaggagt tgtttcgggc gactgaacgt aaagggtttc
     6841 aatcgggtcc cttctcctcc acatagaacg accgctttcg taaaacccac tcctttcccg
     6901 aacgtgtcga ttataccaat tctgagattt tttctgccaa ccggtacgag ggaagaacgt
     6961 aatcggagta cccctcttgg accacggact tgggcatacc gtacacgaca caggtttctt
     7021 ccgattctgc taaaactatt cctttgtaat gtttgatctt gaatgatcct ttggttccgt
     7081 ctcttcccat accggtcatg acaaccccaa tcgttttgtc tttataaagt tctgctgcgt
     7141 gttcgaaggt gaagtctgcc gcgggtctga cgccattgat cttcgggctt tgatccaaaa
     7201 acacacgaat atctcccgat tccgcgcgta gtccgacgtg gtaatctccg ggagctacga
     7261 acacggtacc ttgtttgaga acgtctccgg attgcgcttc tttgacttcg atctctgata
     7321 tacgattcaa cctctgcgcc aaggaggcgg taaacccagc aggcatatgt tgcacgagga
     7381 ccacgggagc cggcaagttt ttgggtaaag caggtatcac ctgatctaaa gacttcggac
     7441 cgccggtgga agacgcgatg aggacgatct tccctttcgc cgtgaaggac ctgctcgttc
     7501 tttttgcaat aggaagcggg cgatactgga ttttcgatgg atcgatcgac gctgcctccc
     7561 ggattttggc caacaaggtg ttgcccataa tacgaaagtt tgtggatatc gaaccggaag
     7621 gtttgggaac aaaatcgatg gcgccgttct gtaaggcttc gatggttata ttcgcgcctt
     7681 cttgtgtgag actgctcacc atgatcaccc tcgtctggat ttttctagag atcaacttca
     7741 aagcctccaa cccgttaagc ttgggcatct cgacatccat cgtgatcaca tccggtttca
     7801 gctggaccgc tttttcgacg gcttccatcc cgtctcgggc ttggccgacg atctctatat
     7861 ctccctgacc atccaagata tctttgatga tcatacgcat aaaagcagag tcatcacaga
     7921 tgagtacgcg aatgatcgcc atgtgtcatc cccttcccta ttaacagcaa taaggttacg
     7981 atgaatagaa agaatgcgaa aagaccgatg agatccggta ggtgaaagag atgttttccg
     8041 aagataatca acccgacaga aacggcgaat acggcggtga ccaacacgat gccggccgcc
     8101 aggtccttcg cctttttgac ttcttcgtgg aaaaacgggt aaaacaggtc tagcagactc
     8161 tctaccagcg a
//
LOCUS       DAOPXN010000096         7888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9193_length_7888_cov_4.811566, whole genome shotgun sequence.
ACCESSION   DAOPXN010000096 DAOPXN010000000
VERSION     DAOPXN010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9193_length_7888_cov_4.811566"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..2299
                     /locus_tag="PKM49_06425"
     CDS             482..2299
                     /locus_tag="PKM49_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNY82484.1"
                     /translation="MTLFLLPGVNLRVGRLELGIGFLKWTMQIKKGAGQHFSPDWDEL
                     PNQTGRLKKEIRRLGKQLEGVNPGFVITVNGKRKGIFSDFLFKNIDKVEEKELYDILR
                     AATAFGSIGPERKGKALGSFLQTLSFLNRRESRAMTTSGYLARLILEIIQPLSIESLC
                     DLCSGMGILGVQALSENPLLRFYGQEIDEKSYFLSVIHFALSGFQNAELRFGDILKNP
                     LLKEEGALLQFDSVAADLPANQYIGDTREMAFPQRFTEGFGRNLSSDWLFVEHALHVL
                     KPGGVGVVVLSTPSLNKAGDSAVRRRLIRQDLVEAVIDLPDTIADASSQPRSALVIRK
                     GKPAERTGKVLMIDVYGQFSTGKDGDAGHWVDKAASIYREGKEIAGISVWRSVEEIER
                     KGALLGASGYALATHPVTEAHPSKPLKSLAVAVFRGIQLSTTQRESAANREGEEVFLL
                     NSGDILDEREFREFRPEIIRFWNRKWTLYALKEGDLVISARGTIIKTMVIQPEHLPAL
                     VSSNVLCVRLKEGAIDPYYLKAFLDSPRGQLILNSVQTGRTIKAINPKSLETVQIPCP
                     GPDRQKQIGDAYRKAQEDFRRGRERYENQLREVFATFEE"
     gene            3392..4513
                     /locus_tag="PKM49_06430"
     CDS             3392..4513
                     /locus_tag="PKM49_06430"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY82485.1"
                     /translation="MEELELTPAQAKEAIRFFFDRMPYLPILLCGPSGIGKTDITRQI
                     ASENQWEYLDIRLAGMLPEDLRGYPKAETWTDVAARSNLGIRGVRRPQIEYVLVDKLT
                     RVFETEGPGILDFEELNRAHPDTHQPIFQLIGDRAMDGRVMGGQWRIIGSINPEDDRN
                     YMVNAMDMAFTRRWLTIRVKPDVNSWLEYAKQNFFHPAVIEYVKANPSMLFKPLTERL
                     TLMPAVWERASKLLYSFKSSQEIMTKGLIPIQLTLGVSVGLEVLDMASKNVCVSITPM
                     DIIRHYASDKALQGAIREKVEAGHMSELARLADALSLTMEEWNINTILFALDLPNDTC
                     QMLISRLRSEVVDFSDPEMKQALAKLYLKMDTFEMDEAI"
     gene            4513..5817
                     /locus_tag="PKM49_06435"
     CDS             4513..5817
                     /locus_tag="PKM49_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HNY82486.1"
                     /translation="MDRQNLTPYPAGKELETAIQNGIKSGCFYSYILSSVDRRIDETA
                     EVVAHVSLCPHRAEWTLVVNPILFTEILEAHPDIDRESILRAILLHEYLHIAHDHFER
                     GFALQEKYSLIERNIAADLAVNSTLEESDKNALKEIGGLFPAQPPFNFPEGLSMEQYL
                     ALLSLGSFDAKISDSKLAGYAKTMKELQAFSDALTQTAGSESVSLKDLEAFASQKLKA
                     RVRASVKNLKEKPLPGLCLPPGIGAGQMNWMVEWAYPKAKISWKHLLKDHLEHSGASG
                     ARVRTYLRSHPREGGGILLKGKTPLKMEQILILLDTSSSMRKEDYNQFIGVIKSLRSE
                     RRSFSIMQWDTARQSEPVPVERFMRLEKPRLRGGGGTNMRAGVLEASKRYPQFKRIIV
                     VTDGHTPYFTKMNPSPVPVIWALTSNARFANADGAVIRLYSK"
     gene            complement(5913..6905)
                     /locus_tag="PKM49_06440"
     CDS             complement(5913..6905)
                     /locus_tag="PKM49_06440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNY82487.1"
                     /translation="MIDYDDIGALASPDETETYWIPADSQDRVAKSSFKEIDYLIRGF
                     VLNKEQVDEKQLEKAIAMHQYVLSPTSVMTRVKAGQFIMGNFENDRKGHPDEKPAHDV
                     TLTYDFWLGMYPVTVWEFNRFCLATSHKRSKEEMEESGPRPVTGVSWWYDVIVFCNWL
                     SLREGLPRAYDEIGNLLDPEGERTEDISVVEGYRLPTEAEWEYAARGGHKKTKRYKYS
                     GSDNLNEVGWYDRNSSSRQRSLSPFPVGKKKPNELGIYDMSGNVWECCSDRMGKYLPE
                     SQVNPIGATRGNARIHRGGSFYHLNEHCRVAYRGASFPDSVSSTRGFRVARTIR"
     gene            complement(7226..7531)
                     /locus_tag="PKM49_06445"
     CDS             complement(7226..7531)
                     /locus_tag="PKM49_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82488.1"
                     /translation="MEIEMDKEFQDFFEKLLGIADPWYIREVEQNEQGIHFHIDFNRG
                     AQFPYKGEMYSVHDTVEKEWWHLNFFQYRTYLHANVPRINTPDGIIQVQVPWVHEGS"
BASE COUNT         1980 a         2013 c         2012 g         1883 t
ORIGIN      
        1 gatcagcccg tacaaaagcg tatcgcgccg tcgcgaaaag aaggggcgct tagccgcgat
       61 gcttgtcctc tctgtgttct tcgcgctctc cgtgtgaacc ctcttttcta aagaagaaaa
      121 gcttacgcct cgatgacttc atagattaaa aagtgcgcga tcgattttgg ccttgtttct
      181 tgattacacc tcatagcttg acacacaccc ggtcattgga atcgcgacga atagtcgtat
      241 aatatggata tcggtaataa attaccgcct ttgcgaagga cagtcgcaac tgaataaaaa
      301 tggcaaacga atcgacgggg tgagagaagc aaggtcctca cggctgtaga ggggaaatag
      361 gacgctttca gagaaacatc cgcgtttcgc tgctttcccg aataggggca aaagaacgga
      421 cgatcacccc ggtagacggt cgattcttaa tcgtacttca cgagaaaggg gagaaccgaa
      481 tatgacgtta ttcttattgc caggcgtaaa cctcagggtg ggtaggcttg aattagggat
      541 aggttttttg aaatggacaa tgcaaataaa aaaaggggcg ggacagcatt tcagccctga
      601 ctgggatgag ttgccgaatc aaacgggacg attaaaaaaa gagatccgcc gccttggaaa
      661 acaattggaa ggagtcaacc ctggctttgt gataaccgtc aacggaaaac gcaaaggaat
      721 tttctcggat ttccttttta aaaacataga taaggtcgaa gaaaaagaac tttatgacat
      781 cctgcgcgcg gcgacagcgt tcggctctat cggcccggag cgaaagggaa aggcgttggg
      841 ctcttttctc caaaccctaa gcttcctcaa tcgtagagaa agtagggcta tgacgacctc
      901 gggctatctg gcgcggctga tcctcgaaat cattcagccg ttatcgatcg agagtctgtg
      961 cgacctctgc tccggcatgg ggattctcgg cgtccaagct ctttcggaaa acccgcttct
     1021 tcgcttctac ggtcaggaaa tcgacgaaaa gagctatttc ctgagcgtga tccatttcgc
     1081 cctttcgggc tttcaaaacg ccgagttgag gtttggcgac atcttgaaaa acccgctatt
     1141 gaaagaggaa ggcgctcttc tccagttcga ctccgtcgca gcggaccttc ccgcgaacca
     1201 atatatcggt gatacgcggg agatggcgtt tccgcagcgt ttcacggaag gattcggcag
     1261 gaacctgtcg agcgactggc tcttcgtcga acacgcgctc cacgttttga aaccaggcgg
     1321 cgtgggcgtt gtcgtcctct ctacccccag cctgaacaag gccggcgaca gcgcggtgag
     1381 gcggcgcttg attcggcaag acctcgtcga agctgtcatc gatcttccgg acaccatcgc
     1441 cgatgcctcg tcgcagcccc ggtccgcgct cgtgattcga aaagggaaac ccgcagaaag
     1501 gacgggaaaa gttttgatga tcgacgttta cgggcagttt tcgacaggaa aggatggcga
     1561 tgccggtcat tgggtcgata aggcagcttc tatttaccgg gaaggaaagg agattgcggg
     1621 aattagcgtt tggcgctctg tcgaagaaat agaacggaaa ggcgcccttc tgggcgcttc
     1681 agggtatgcg ctagccactc atccggtaac ggaagcccat ccctccaaac ccctaaaatc
     1741 gctcgccgtg gctgtctttc gaggaattca gctcagcacg acccagcgcg aatccgccgc
     1801 gaatcgggaa ggggaagagg ttttcttgct caactccgga gatatccttg acgagcgtga
     1861 attcagggag ttccggcctg aaataatacg gttctggaac agaaagtgga ctctctacgc
     1921 gttaaaggag ggggaccttg tcatttccgc ccgcggaacg attataaaga cgatggtgat
     1981 ccagcccgaa cacctgcccg cgctcgtctc ttcgaatgtt ctgtgcgtgc gtttgaaaga
     2041 aggagcgatc gatccgtact atcttaaggc ctttttggac agccctcgcg gtcaactgat
     2101 actgaatagc gtgcaaacag gaaggaccat caaagcgata aacccgaaaa gcttggaaac
     2161 cgttcagatc ccctgccctg gccctgatcg gcaaaagcaa atcggcgacg cttaccggaa
     2221 agctcaggag gatttccgcc gcgggcggga acgttacgaa aaccagttaa gggaggtctt
     2281 cgcgacattt gaagagtgat tccgaagctt gtgtgactta accaacgaaa aataggatta
     2341 ctttggcaga tgcggaagcg aactgagaat aacgggtagc gacactaaac cccgtattgc
     2401 ctccaaaaaa tgaattccgg cggaaaagtt cgggaagaac caatgactaa tgcctaagga
     2461 gccgattcgg ggcgtatcga gaaactgttg ttaggcgaca ggttagtgtg ggtcaagcta
     2521 tggggcgcaa tcaagaaaca tggccagatt cgaccgcgcg atttttaatc cgcgtagtca
     2581 ccgcggcgta agcgccactt ctttacgcgg ggccgacttc cagtcggcct gcctctctgg
     2641 gaacgccaac tttctagttg gcatattttg aaaaagaaag acagcagagc gtatcgcgtg
     2701 actttgaggg cttccgaagt cagcccgctg gttacgccgc gatgaacctc ttagttattt
     2761 caccccataa cttgacccac acggcaggct agagccgttg cttcgggcgg agtgcggaga
     2821 gcgcgagtgt aaaacagaat gttttcctga tttttagcta caccatctac caaattcccg
     2881 gaacaagttt cctctttcgg gaaacatcgt gatttaaaag ccctacctgc gatgattctc
     2941 gggttaacat atgaaggttt aggggttcgc gaagaaatga ccgcttctga cgcgtttttg
     3001 aacacggtac gcaacgtgaa atcctttgtg ctcctttcgc ggctcctgtt tccgaaaaat
     3061 cctcttcccc ggtgaaaaga aagctgaacc ttatctttcg ctcggcgttc acgacgacac
     3121 tcaagacacg gcttttagga cggattattt cgtaagtagt taggtatttt attacctaat
     3181 atcactactc aactatagaa ttctattcca tattggttga caaaaatcga atcgtatggt
     3241 atactctggt tatcgatacg ttcggagacg tcttatactc cgtatttctg cgagacgact
     3301 ctcgaaaggg atcgacttcg gatttctgcg ccatacagat cgttttgaat actgtcgttt
     3361 atccttgaat atcgcgaaaa aggagggttt gatggaagaa ctcgaactga cgcccgcgca
     3421 agccaaagaa gccatacgtt ttttctttga ccgaatgccg tatttgccca tccttctttg
     3481 cggcccgtcg ggcatcggaa aaaccgatat cacccgacag atcgcctcag aaaaccagtg
     3541 ggaatatctg gatattcgac tggcggggat gcttccggaa gacctgaggg gatatccgaa
     3601 agccgaaacg tggaccgatg tcgcagccag aagcaacttg ggcattcgag gggtcagacg
     3661 gccgcagatc gagtatgtgc tggtggacaa actcaccaga gtcttcgaaa cggaggggcc
     3721 ggggattctg gattttgaag aactcaacag agctcatccc gacacgcatc aacccatttt
     3781 ccagttgatt ggtgaccgcg cgatggatgg ccgcgttatg ggaggacagt ggaggatcat
     3841 cggttccatc aaccccgaag atgacaggaa ctatatggtg aacgccatgg atatggcttt
     3901 caccaggaga tggttgacca ttcgtgtcaa gccggacgtc aacagctggc tggaatacgc
     3961 caaacagaat ttctttcatc ccgcggtgat tgaatacgtg aaagccaacc catcgatgct
     4021 ttttaaacct ttaaccgagc gcctgaccct tatgcctgcc gtttgggaaa gagccagcaa
     4081 gctcctgtac tcgttcaaaa gctcgcaaga gattatgacc aaaggcctga ttcccatcca
     4141 gttgactctg ggcgtgtcgg tcggcctcga agtgctggat atggcctcta aaaacgtttg
     4201 cgtttccatt actccgatgg atattattcg acattacgcc tcagacaagg ctttgcaagg
     4261 cgccatccga gaaaaggtcg aagccggcca tatgagcgaa ctggctcgat tggccgatgc
     4321 cctgtccctt acgatggagg agtggaatat caacacgatc ctattcgccc tggaccttcc
     4381 caatgacacc tgccaaatgc tgatcagtag gcttcgttca gaagttgtgg acttttctga
     4441 ccccgaaatg aaacaggcgc tggctaagct ttaccttaag atggacacct tcgagatgga
     4501 tgaggcgatc taatggaccg acagaatctc acgccatatc ccgctggaaa agaacttgaa
     4561 acggcgattc agaatggcat taagtcaggc tgtttttaca gctacattct ctcttcggtc
     4621 gaccgcagga tcgatgaaac ggcagaagtc gtcgcccacg tctccttgtg cccgcatcga
     4681 gcggaatgga cactggtggt caaccccatc ctcttcaccg aaatcctgga agcccatcct
     4741 gatatagatc gggaaagcat attaagagcg attttgcttc acgagtacct acatattgcg
     4801 cacgaccatt ttgaacgcgg tttcgcctta caggaaaaat actctctcat cgagcgcaac
     4861 atcgcggccg atctggctgt gaacagcacg ctagaggaat cggataagaa tgcgctgaaa
     4921 gaaatcgggg gtctttttcc ggctcagcct cctttcaatt tccctgaggg gttgtcgatg
     4981 gagcaatacc tggccttgct gtctttagga agctttgacg ccaagatttc ggattcgaag
     5041 ctggcgggtt acgcaaaaac gatgaaagag ctccaagcct tctccgatgc gcttacccaa
     5101 accgcgggga gcgaaagtgt ctctctgaag gatttggaag cgtttgcatc gcaaaaacta
     5161 aaagcgcggg tcagagcgtc cgtcaagaac ttgaaagaaa aaccgttacc aggcctatgc
     5221 ctgccgccgg gtattggagc gggacaaatg aattggatgg tcgagtgggc ttatcctaaa
     5281 gcgaagattt cgtggaaaca cctcttgaaa gaccatttgg aacattctgg ggcgagcggc
     5341 gcgcgcgttc gaacctatct ccgttctcat ccccgcgaag gcgggggcat cctcctaaaa
     5401 ggaaaaaccc ctttaaaaat ggaacagatc ctcatcttgc ttgacacctc cagctcgatg
     5461 cgtaaagagg actacaacca gttcataggc gtgatcaaat cgcttcgtag cgaacggcga
     5521 agcttctcga ttatgcagtg ggatacggct cgtcagtcag agcctgttcc tgtcgagcgg
     5581 ttcatgcggt tggaaaagcc tcgattacgt ggcggaggag gcacgaacat gcgcgcggga
     5641 gtgctagaag cgtcgaaacg gtacccacag ttcaaaagga tcattgtggt cacggatgga
     5701 catacgccat acttcacaaa aatgaaccct tcgcctgtcc cagtgatctg ggcgctcact
     5761 tcgaatgcgc ggtttgcaaa cgccgatgga gcggtgatcc gcttgtattc gaagtgacat
     5821 cgactgcgct acgaatgtga gaaagagcga tgacggggat tgcgccctac gaagaactgg
     5881 tatactcgac tttttacatc cgtacgaaaa cttcagcgaa tcgttctcgc gacgcggaat
     5941 ccgcgagtgg aagatacgct atccgggaag cttgcgccac ggtaagcgac cctgcaatgc
     6001 tcgttcaagt ggtaaaagga accgccgcga tggattctgg cgttgccacg ggtcgcgccg
     6061 atagggttca cctgagactc gggcaggtac ttccccattc gatcagagca acattcccac
     6121 acgttgccgc tcatatcata aatacccagc tcgttgggtt tcttcttccc aaccgggaag
     6181 ggggacaagc tacgctggcg tgatgatgag tttcgatcat accatcccac ttcattaaga
     6241 ttatcgcttc cggaatactt gtacctcttc gttttcttat gaccgcccct tgcggcatac
     6301 tcccattccg cttctgtagg caaccggtag ccttccacta cgctgatgtc ctctgtcctc
     6361 tctccttccg gatccagaag attcccgatc tcgtcataag cgcgcggtag tccctctctc
     6421 aggctcaacc aattacaaaa gacgatcaca tcgtaccacc aagaaacgcc ggtcacaggg
     6481 cgcggtccgc tctcctccat ttcttctttc gatcttttat ggctcgtcgc gagacagaag
     6541 cggttaaatt cccagacggt cacaggatac atgcccaacc agaaatcgta ggtcaaggtg
     6601 acgtcgtggg ccggtttttc atcggggtgc cctttacgat cgttttcgaa attccccatt
     6661 ataaattggc ctgctttgac gcgcgtcatc acggaagttg gggacaaaac gtattgatgc
     6721 atcgcgatcg ctttttctaa ctgtttttca tcgacctgtt ctttattcag aacgaagccc
     6781 ctgatgagat agtctatttc cttgaaagag ctcttagcta cccgatcctg tgagtccgcg
     6841 ggaatccaat aggtttcggt ctcatccggg ctggcaagtg ccccaatatc gtcgtagtcg
     6901 atcatacgga cctccttttc gagacaagaa ccaaaaagcg agtagtcttg tgaaagtcca
     6961 tccttctccc ttgctcacgt ttcaaaagcc tggcgcattt tccaataatt acgcttttga
     7021 gcgtgaagga taactgcccc tatatccccg actgacgcgg agaaacgagt tcagcctcgt
     7081 tgtgagaaaa tatcctatga agacttgcga tgggtcaaag tcactagggc tgtcaacggt
     7141 ctttcgggcg ctttcgtccg ttggcaaaac cggtaatctg gcccttatct acgcgccgct
     7201 ttgcctattg agagtgttgt gtgaatcagc ttccttcatg cacccagggt acttgtactt
     7261 gtatgatgcc atccggcgta ttgattctcg ggacattcgc gtgcagatag gtacgatact
     7321 gaaaaaagtt caaatgccac cactcctttt caacggtatc gtggacacta tacatctcgc
     7381 ctttgtaggg aaattgcgct cctcgattga aatcgatgtg gaagtgtatg ccctgctcat
     7441 tttgctctac ctcgcggatg taccacggat ccgctattcc tagcagtttt tcgaaaaagt
     7501 cctgaaattc cttgtccatt tctatttcca tatccgaatc ctcctaaagg gcattattca
     7561 taggtaattc tatcacactc attcaatatc ggatatatta gcgtgggcca agttatgagg
     7621 ttacataact aagaggttca tcgcggggta accaacggga ccccgcgatc tgctctttcc
     7681 ccttctcaaa cgcgcatagt gccgtcgcga aagaaaaagc gcttacacct cgatcaccat
     7741 gtgggatcat cgcggggtaa ccaatgggac cccgcgatct gttctttctc cttctcaagc
     7801 gcataaagcg ccatcgcatt aagaaaaaac gcttacgcca cgatcacctt atgggatcat
     7861 cgcggggtaa ccaacgggac cccgcgat
//
LOCUS       DAOPXN010000097         7836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9264_length_7836_cov_4.542732, whole genome shotgun sequence.
ACCESSION   DAOPXN010000097 DAOPXN010000000
VERSION     DAOPXN010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9264_length_7836_cov_4.542732"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKM49_06450"
     CDS             <1..319
                     /locus_tag="PKM49_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82489.1"
                     /translation="YDPSMRLMSFSKAYRAVAERRSAYAAMTVWGYSGVRGFYDPSMR
                     LMSFSKAYRAVAERRSAYAAMTVWGYSGVRGFYDPSMRLMSFSKAYRAVAKRRGAYAA
                     MTT"
     gene            499..936
                     /gene="rpsF"
                     /locus_tag="PKM49_06455"
     CDS             499..936
                     /gene="rpsF"
                     /locus_tag="PKM49_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNY82490.1"
                     /translation="MRIYELMFIADPRVDEEGRAALVEKVQKVIVEKAAGTIEKVDRW
                     GIRKLAYKLPKTKLTEGDYTVVLFRSNGEKLQEIDVLFQVTPELIRKQIVRREDLEKK
                     ERKEWLKTRDNPVEEMSVSDGSSSGMKEETIEPVFEVEGESQA"
     gene            933..1418
                     /gene="ssb"
                     /locus_tag="PKM49_06460"
     CDS             933..1418
                     /gene="ssb"
                     /locus_tag="PKM49_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNY82491.1"
                     /translation="MSISYNRVVLVGRLTRDPESKYTTSGSHIVKFSIAVDRIPTATQ
                     AEPGADFIPITAFGKTADFAANYLSKGRLILVEGRLNVNKYQAQDGTNRTFTDVIADT
                     LRFMETKGATQREPYRQPRAEETPEEAFPPNPMNEELSETTLTPSVNEDGPSNDDEIP
                     F"
     gene            1434..1703
                     /gene="rpsR"
                     /locus_tag="PKM49_06465"
     CDS             1434..1703
                     /gene="rpsR"
                     /locus_tag="PKM49_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNY82492.1"
                     /translation="MGKVTRGGSRSGDAKNKKFRRRKVKKCQLCVKKMDFVDYKEVNF
                     LKEYISDKGKILPKRINGNCAKHQRFIKDAIKRARQMALVPFTRD"
     gene            1858..3306
                     /locus_tag="PKM49_06470"
     CDS             1858..3306
                     /locus_tag="PKM49_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNY82493.1"
                     /translation="MRVAVIGAGVVGSLIARCLTRYRCEVHIFEKEEDVGWGITKANS
                     AVIHGGYDDPPGSVRARFCAKGNAMYSSLSEELGFELKRIGSFVLVQAPEDFPYLETL
                     KAQALDNGLSGCAILPAQKVRDLEPHIADTVLGGFWCPSAGITEPWMVAIGAVENAVK
                     NGARLHLSEAVTGLVTAEGSRGRRVVALKTVRGEYPIDIVINASGLWAEELSTLAGAY
                     CPPLHPRKGEYLLFDKFDPPLVRSILFPIPSSVSKGILVLPTVDGGLLLGPTATDLPK
                     EAREETSTTREGLSEVLYSARKLVPTLDPQWTVKTFAGLRPESPQKDFAIGKTEVEGF
                     IQAAAMRSPGLTAAPAVAEHVVEEVLRRECGYSLESNDRFTPVNPPFVNVSNLPLEEW
                     EKKIETDPLYGKIACVCNKVTEGDIRDAVRRGATTVDGVKFRTRASFGRCQGGFCLAK
                     IAEILARESGRSLGEVKMRSGRTELLRGRVRE"
     gene            3303..4559
                     /locus_tag="PKM49_06475"
     CDS             3303..4559
                     /locus_tag="PKM49_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY82494.1"
                     /translation="MREVITDTLVIGGGASGMAAAVECVERGCRTILVEREESIGGVL
                     NQCIHNGFGLHVFKEELTGPEYAQLFKSQIHQKGVEIQPDLFVVNIDPKRREVVTMGK
                     GGCVVYRPRALIMTTGARERPFSSLRIPGTRPAGIYTAGVAQRFVNLYNRLPGDRAFI
                     LGSGDIGLIMARRLTLEGMKVLGVAELMPHPGGLARNIAQCLDDFDIPLRLSTSVVEV
                     RGRERLEGITLAKFDPVTLQRIPGTEERVDCDTLILSVGLLPQNELIESFVETEPVNR
                     GVKVDSFMRTTHLWIFAAGNNVAIYDLVDFVTLDGKIAGQSAARMVRDGFLPEKRIPL
                     ARSEQIGTLIPSFCCVEGQPFTIYFRSKKTMKKARVSIGPGIFETVRLGIKPSEMVDI
                     PFTVKHFGKLKQLGLTKIEVSIKEVP"
     gene            4559..4912
                     /locus_tag="PKM49_06480"
                     /pseudo
     CDS             4559..4912
                     /locus_tag="PKM49_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015552878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
     gene            5469..6851
                     /locus_tag="PKM49_06485"
     CDS             5469..6851
                     /locus_tag="PKM49_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNY82495.1"
                     /translation="MKDIDLTAYRDLFRSHTEMRVQESTQSSFSIINGKVEQNIENSS
                     AGVSARVYHKGVWGFASTPVIDHDSVGEVVRAATANAKAFQSLSKTDKPPLEAIPSRS
                     SFSPKRVKEPFSKKENLDRLLAIDAYIAKKYPDLKSRALVTRSQQIEKRWITSFGSEA
                     FSSYPRAHIYVTLTRDKNGDSIENTEVFGGFGYFEEQFDDLCGIHRRIDELYEHLRKK
                     AEGDYPKPGFQDVVLSPYVGGILAHEAIGHTVEADLVKNGSIARDFLNEKVASDIVTM
                     VDFAHHYKGELLRVPVFTDDEGTEAIDAVLIDQGVLRGYMHNLESAHEFGCAPTGNAR
                     GFEFSDEPLIRMRNTAILPGNSSLEEMIASVDEGYYLMKTMNGQADSTSEFMFGIVQA
                     YEIKNGKLGRALRDFTVSGVAFDMLKTITMLSSEMAWENSGYCGKKQPMIVSTGSPAI
                     KCKMLIGGRE"
     gene            6851..>7836
                     /locus_tag="PKM49_06490"
     CDS             6851..>7836
                     /locus_tag="PKM49_06490"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNY82496.1"
                     /translation="MERVVSKALVEQALWLLKKAGATQAEVKLALSNKQELNLANNVM
                     SLYRTTQDIALELVAFSGYKKSVKRINQIEDTQVEKAVLEVVETAKGSQEDEANAIAE
                     YQPAQRFQTGPEACDFERLYGKLDAFIQYARKETPYVNLEEGAAEFLLKEHFHMNSNG
                     LDNYAKTAFYNFGVVFIAVKEKARSSFNYTGLLQEELNQEFYDAGSISELMRQSEQQL
                     DPRSIPANFVGDVVFTPDCLADILEVIVANLKDRPIHTGISIFANKLHRPVAHPSLTL
                     LANPVGDGMPDKSFFTENGYLAKNQAIIENGVLNTYLLSLYGARKTGFARSET"
BASE COUNT         1948 a         1967 c         2293 g         1628 t
ORIGIN      
        1 ttacgatccc tcgatgcgcc ttatgtcttt ctcaaaagcg tatcgcgccg tcgcggaaag
       61 aagaagcgct tacgccgcga tgaccgtgtg gggttatagc ggggtaagag gtttttacga
      121 tccctcgatg cgccttatgt ctttctcaaa agcgtatcgc gccgtcgcgg aaagaagaag
      181 cgcttacgcc gcgatgaccg tgtggggtta tagcggggta agaggttttt acgatccctc
      241 gatgcgcctt atgtctttct caaaagcgta tcgcgccgtc gcgaaaagaa ggggcgctta
      301 cgccgcgatg actacatagg ctaaaaatcg tgcgatcgat tctgtccttg tttcttgatt
      361 gcaccccata atttgaacca gacctgcagt tgcaattgct ccgttttatg atataatccg
      421 cggaaggaag gcttggcctt ccagacatcc tgtctcgttc cagaaacgag accattttta
      481 tgcccgtagg aggttaacgt gagaatttac gaactgatgt tcatcgcgga tccccgagtc
      541 gatgaagagg gtcgcgccgc gctggtggag aaggtccaga aagtgatcgt cgaaaaggcg
      601 gccggtacga tcgagaaggt ggatcgatgg ggaatccgaa aactggctta caagttaccc
      661 aaaacgaaac tcaccgaagg agattacacc gtcgtcttgt tccgttctaa cggagagaag
      721 ctgcaagaga tcgatgttct cttccaggtc acgcctgaat tgatccgcaa gcaaatcgtg
      781 cggcgagaag acctggaaaa gaaagagcga aaagagtggc ttaaaacgag ggacaatcca
      841 gtggaagaga tgtccgtctc cgatggctcg tcttccggaa tgaaagaaga gaccattgaa
      901 cccgtctttg aagtggaagg agaatcgcag gcatgagtat cagttataac cgggttgtgc
      961 tcgtcgggag gttgacgcgt gatccggaaa gcaagtacac cacatcgggg tctcatatcg
     1021 taaaattctc gatcgcggtc gatcggattc ccactgccac acaagccgag ccgggagcag
     1081 acttcatccc gatcaccgca ttcggaaaaa ctgccgactt tgccgcgaat tacctctcca
     1141 aggggcgcct gattttggtc gagggtcgtt tgaacgtcaa taagtatcaa gcccaagacg
     1201 gcacgaacag gacctttacc gatgtgatcg cggataccct tcggtttatg gaaaccaaag
     1261 gggccactca gagggagccc tataggcagc cacgggcgga ggagactccg gaggaagcgt
     1321 tccctccaaa tccgatgaac gaagaactga gtgaaacgac gctcacgcct tcagtaaacg
     1381 aagacggccc gagcaacgac gacgaaatac cattctaaag gaggaaaagg gttatgggta
     1441 aggtaaccag aggcggttcc agaagtgggg acgcgaagaa taaaaaattc cgaagaagaa
     1501 aagtaaaaaa atgccaactg tgcgttaaaa aaatggactt cgtcgactat aaagaggtca
     1561 acttcctgaa agaatatata agcgataaag ggaagatcct tccgaagaga atcaacggaa
     1621 actgcgccaa acatcagcgt tttatcaaag acgcgatcaa gcgcgcccgt caaatggctt
     1681 tagtaccctt cacaagagac tgacacggtt ttaagcaggc cctcgcggtt aaggcgagga
     1741 atagagaaga actgaatagt tagggcagag aaagagagag cgcccgcagg ttgatccctc
     1801 gtatccggtt cgagggtgcc ggggcgcttt tctattggag gaatgatgcg cgaattattg
     1861 agggtcgcag tgatcggggc gggtgtcgtc gggtctttaa tagcgcggtg cctgacccgg
     1921 taccggtgtg aagtgcatat atttgaaaaa gaagaagacg tcgggtgggg aatcacgaag
     1981 gcgaactcgg ccgtgataca cgggggatat gacgaccccc ctggaagcgt tcgcgcgcga
     2041 ttttgcgcca aaggaaacgc gatgtattcc tcgctttccg aagaactcgg ttttgaactg
     2101 aaacgcatcg gttcgttcgt attggtgcag gcacccgagg atttccccta tctggagacg
     2161 ttgaaagcgc aagcccttga taacgggttg agcggttgcg ccatccttcc ggcgcagaag
     2221 gtgagggacc tggaacccca catcgccgac accgtcctcg gcggcttttg gtgtccgtcc
     2281 gccggcatca ccgaaccctg gatggtggcg atcggcgccg tcgaaaatgc ggtcaaaaat
     2341 ggcgcgcgtc tgcacctttc agaagcggta actgggttag tcaccgccga agggtcgaga
     2401 ggacgccggg tcgtcgcgct gaagacggtt cggggtgaat acccgatcga tatcgtcatc
     2461 aacgcgtccg ggctctgggc cgaggagctc tcgacgctgg ccggagccta ttgcccgccc
     2521 ttgcacccga gaaaggggga gtatttattg ttcgataagt tcgatccgcc gctcgtcagg
     2581 tccatcctct tccccatccc gtcctccgtt tcgaaaggca tactggtttt gcctaccgtg
     2641 gacgggggat tgctgctcgg cccgaccgcg accgatctgc caaaagaggc gcgagaagag
     2701 acgagcacga ctcgagaagg gttgtccgaa gttctctatt ccgcgcggaa gttggtcccg
     2761 acgctcgacc cacagtggac ggtgaagacg tttgcgggct taaggcccga aagtccgcaa
     2821 aaggattttg ccatcggaaa gaccgaggtc gaagggttca ttcaagcggc ggcgatgcgt
     2881 tcccccgggc ttacggccgc accggcggtc gccgaacacg tggtggaaga agtgctccgt
     2941 cgggaatgcg ggtactcctt ggagagcaat gaccgattca cgccggtgaa tccgcctttc
     3001 gtcaacgtca gcaatttgcc tttagaggaa tgggaaaaga agatagaaac cgacccgctt
     3061 tacggaaaga tcgcttgcgt gtgcaacaag gttaccgaag gtgatattcg agacgcggta
     3121 agaaggggcg cgacgaccgt cgacggggtt aaattccgga cacgggcttc tttcgggcgt
     3181 tgccagggcg ggttttgtct ggctaagatc gcggagatcc tggcaaggga gtcggggcgt
     3241 tctctaggag aagtcaagat gcgttcgggc agaaccgaac tcctccgggg gagggtgaga
     3301 gaatgagaga ggtcatcacg gacacgctcg tgatcggtgg aggggcatct ggaatggcgg
     3361 ccgctgtgga atgcgtcgag cggggctgcc gaacgatcct cgtggaaagg gaggagagta
     3421 tcggcggcgt cttgaatcag tgcatacaca acgggttcgg cctccacgtg ttcaaagagg
     3481 aattaaccgg tcccgagtac gcgcagctat tcaaatccca gatccatcaa aaaggggtgg
     3541 aaattcaacc cgatctcttc gtcgtcaaca tcgacccgaa acgtcgggag gtggtgacga
     3601 tggggaaggg cggatgcgtc gtctaccggc ccagggcgtt gattatgacg accggggcgc
     3661 gcgaacggcc ctttagcagt ctgcggattc cgggaacgag gccggcgggc atctataccg
     3721 ccggagtcgc gcaacggttc gtcaacctct acaaccggct gcccggcgat cgcgcgttta
     3781 tcttggggtc aggggacatc ggtctcatca tggcccgcag attgacgctc gaggggatga
     3841 aggtgctcgg tgttgccgaa ttgatgccgc atccgggagg actagcccga aacatcgccc
     3901 aatgcctgga cgacttcgac atccccttgc gtttgtcgac cagcgtggtg gaagtccggg
     3961 gccgcgaacg tttggaaggg attacgctcg ccaaattcga tccggtgacg ctgcagcgca
     4021 ttccgggcac ggaggaaagg gtcgattgcg acaccttgat cctatcggtc ggcctccttc
     4081 cgcagaacga actgatcgaa tcctttgtcg aaaccgaacc cgtcaaccgt ggggtgaagg
     4141 tggactcgtt tatgagaacg acgcacctct ggattttcgc tgccggaaac aatgtcgcca
     4201 tctacgattt ggtcgatttc gtgacattgg acggcaagat cgccggtcaa agcgccgcga
     4261 ggatggttcg tgacgggttt cttccggaaa agcgcattcc cttggcgagg agcgagcaga
     4321 tcggcacact gattccgtcg ttctgctgtg tggaagggca gcccttcacg atctatttcc
     4381 gttccaaaaa aacgatgaag aaagcccgcg tctcgattgg acccggaatc ttcgaaacgg
     4441 tccggttggg aatcaaaccg agcgagatgg tcgatatccc gttcaccgtg aagcattttg
     4501 gaaagttgaa acaattagga ttgacgaaaa tagaagtctc cattaaggag gtgccataat
     4561 ggagaagaga gggttgactt gcatatcttg cccgttgggc tgtgacttag aagcggaggt
     4621 tagcgccgat accgtgacag tgaccggcaa ccgctgcgag aaaggggtcg tctacgccaa
     4681 agccgaggtc ttgaatccgc aaagggttgt cacctcgaac gttctggtag aaggcggtct
     4741 gagtcccttg gtttcggtaa aaaccagcgc gccgattccg aaaggattga ttgggaagat
     4801 gatacaggaa ttgaaaaaca tcaggctcca cgcgccgatc cgcattgggc aggtcctcat
     4861 ccgagacttg ctcggaaccg gagtggacgt cgtcgcaacg cgtgcggtcg agcttctccc
     4921 cacggcgcaa tcggggccgt ctcctatacc tatctcacat cccatcgacg tgtgcgttaa
     4981 gttatagggt gacataacag gaggttaatc gcggggtaac cagcgggctg acttcggaac
     5041 cccacgaagt ctcgcgatct gctctttctc cttctcaaac gcgtatcgcg ccgtcgcgaa
     5101 aagaaagggc gcttacgccg cgatgaccgt gtggggttat cgcggggtaa gcggttttta
     5161 cgccccccga aaaacttgcc tacttgaaag ttggcgtccc cggaaattat gcggaactag
     5221 agtttggcgt tcccaagaat ctctcctccg tgcgctctgc gtgaatccga tttttttcgc
     5281 gccctcgcga aaagaagggg cgcttacgcc gcgatgacca cacggactga aaaccgtgcg
     5341 atcgaatctc gccttgtttc ttgatttgat cccataactt gacccacacc ccatcgacgg
     5401 aatagagacg gattatagta taattgtcta taaaattccg tcgaacgaaa ccggaaaagg
     5461 gggcttatat gaaagatatc gatttgacag cataccggga tctgtttcgt agtcacacgg
     5521 aaatgcgtgt ccaggaaagc acgcaatcct ctttctcgat cattaacgga aaagtcgagc
     5581 aaaacataga aaactccagc gctggcgtca gcgcgagggt ttaccacaag ggggtgtggg
     5641 gattcgcgtc gactccggtg atcgatcacg actcggtcgg agaggtggtg cgcgccgcga
     5701 ccgcgaacgc gaaggctttc caaagccttt ctaagaccga taagccgcct cttgaggcga
     5761 ttccctcacg gtcttccttc tctccaaagc gcgtgaagga gcccttctca aaaaaggaga
     5821 atctcgaccg gcttttagcg atagacgcgt atatcgcaaa aaaatacccg gatttgaagt
     5881 ctagggcgct cgttacccga agtcagcaga tagagaaacg ctggatcacc tcttttggct
     5941 cggaagcctt ctcctcctac cctcgagccc atatttacgt gacgctgacc cgcgacaaaa
     6001 acggcgattc catcgagaat acggaggtat tcggcggatt cggctacttt gaagagcagt
     6061 tcgacgacct ctgcggtatc caccgcagga tcgatgagtt gtacgaacac ctaagaaaaa
     6121 aggcggaggg ggactacccg aaaccgggat tccaagatgt ggtgttatct ccttacgttg
     6181 gcgggatact ggcgcacgaa gcgatcgggc atacggtgga agccgacctt gtgaaaaatg
     6241 gctcgatcgc cagagacttc ctgaatgaga aagtcgcctc ggatatcgtg acgatggtgg
     6301 acttcgccca tcattacaag ggggaactgc tacgtgtccc ggtgttcacc gatgacgaag
     6361 gcaccgaagc tatcgacgcg gttttgatcg accagggcgt gctcaggggg tatatgcaca
     6421 atctggaatc ggcccacgag ttcggatgcg ccccgacggg aaacgcgagg ggattcgaat
     6481 tttcggacga accgttgatc agaatgcgga acaccgccat attacccgga aacagctcgt
     6541 tggaagagat gatcgcctcc gtcgacgagg ggtattacct gatgaagacg atgaacggtc
     6601 aggctgattc gaccagcgaa tttatgttcg gtatcgttca ggcctacgag ataaaaaacg
     6661 gaaagctcgg acgcgcgctc agggatttca cggtttcggg cgtcgctttc gatatgctca
     6721 aaacgatcac tatgctttct tcggagatgg cgtgggagaa ttcgggctat tgtgggaaaa
     6781 agcagcctat gatcgtatcg accgggtctc cggccataaa gtgtaaaatg ctgatagggg
     6841 ggagagaata atggaacgcg tcgtatcgaa agccctcgta gaacaagcct tgtggttgtt
     6901 gaagaaagcg ggcgccacgc aggcggaagt gaagttggcg ctgtcgaaca aacaggagtt
     6961 gaatctggcg aacaacgtga tgagcctgta tcggacgaca caagatatcg ccctagagct
     7021 cgtcgcgttc agcgggtata aaaagagcgt gaaacggatt aaccagatag aggacaccca
     7081 agtcgaaaaa gcggtgttgg aagtggtgga aacggcgaaa ggctcccaag aggatgaggc
     7141 gaacgcgatc gcggaatatc agcccgccca gcggtttcaa actggaccgg aggcttgcga
     7201 tttcgaaagg ctctacggga aactggacgc cttcatccaa tatgccagga aagaaacgcc
     7261 ctatgtcaat ctcgaggaag gggcagcgga gttcttgctc aaagaacatt tccatatgaa
     7321 ctccaacgga ttggacaact acgcgaaaac cgcgttttat aattttgggg ttgtttttat
     7381 cgcggtgaaa gaaaaggcgc gttcctcttt caactatact ggtctgctac aagaagagct
     7441 gaatcaagag ttctacgacg cgggatcaat ctccgagttg atgagacaaa gcgaacaaca
     7501 gctcgaccca aggagcatcc cagcgaattt tgtcggcgac gtcgtcttca cgcccgattg
     7561 tctcgccgac atcttggaag tgatcgtcgc gaacttaaaa gaccgcccca tacacaccgg
     7621 catatcgatc ttcgccaaca aactccaccg cccggtcgcc cacccttcct tgaccctcct
     7681 cgcgaatccg gtcggcgatg ggatgccgga caagtctttt ttcacagaga acgggtactt
     7741 ggcgaaaaac caagccatca tcgaaaacgg tgttttaaat acctatctcc tcagtctcta
     7801 tggcgcgaga aaaacgggtt ttgccaggtc cgagac
//
LOCUS       DAOPXN010000098         7808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9312_length_7808_cov_5.481491, whole genome shotgun sequence.
ACCESSION   DAOPXN010000098 DAOPXN010000000
VERSION     DAOPXN010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9312_length_7808_cov_5.481491"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PKM49_06495"
     CDS             <1..287
                     /locus_tag="PKM49_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82497.1"
                     /translation="TKDAEELLTFYDFPAAHFQHIRTTNPIESSFSTIRLRTKKMRNC
                     GNRKTTLAMMYELSQQVEKGWRKLRGFKEIQYVLEGNQYLNGSRMKNVVV"
     regulatory      1047..1145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1205..2182
                     /locus_tag="PKM49_06500"
     CDS             1205..2182
                     /locus_tag="PKM49_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY82498.1"
                     /translation="MNRYRYCLAILLGCLCVIGFSGLRVYAYESLNWLKDTLIPIFEE
                     QTGIEVTLVTLGDAGNILSRLILEKRRPLSDVVLGLDQSLAYRALSEGLLEPVAPRNA
                     DRIVDKSLFFEPEGYLYPFDYGALAIIYDPDRLPDPPTSFRELTTRPKSLLIQDPRNS
                     STGLAFFLWTVAVYREDWREYWKALRPAILTLTTGWSEGFAKFEAGEAPMMVSYATDG
                     AYSYHAYGSDRYKAFIPMEGAFYQTEYVGVTKGTENPEQARLFVEFVLSERFQSEVPL
                     NQWMFPTTPVELPEAYEHAVTSSIHVSISAAEVSKNLDQWLAEWEEIMY"
     gene            2364..3722
                     /locus_tag="PKM49_06505"
     CDS             2364..3722
                     /locus_tag="PKM49_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNY82499.1"
                     /translation="MALCPAYYLYRRRNGLSALLESAIFIPFFLPPVSTVIAFSLLFS
                     TNGLLSRLGIHIPILYTMWAILVAHAFYNSPIFVKYIGAALRTIPPALEEAALCEGAG
                     RWARFWRIEGPLVLPAAAKAFFLCFAYSFMSFAVVLNLGGLQYSTLEVAIAGALRGQF
                     DFSRALTYAIAQFGCLFLLNALLSLIPEVSTGGAPLSLSRERPLRLTTVSSALYAAFE
                     YGIVLIGLLGLCYDFRLGAFSLEPFLRLFSADFNRRFPVIRSLINTFGVASLAAIGAV
                     LAAFLFGKARGNWAQRVILPTLGVSSAFLAMALLYLHILYRLPLFLLLTLGYLLSTLA
                     VAYALMRPMVRNFPLHWVESAKIDGASRLQVFRSIELPLLFPVFAAVFFQVFAIVFGE
                     FTLPYTMQIRDYLPLASVVNYAMDAQRYYRESAAFSGMNTAIVGALFLVSNALIQRRR
                     VI"
     gene            complement(3758..4771)
                     /locus_tag="PKM49_06510"
     CDS             complement(3758..4771)
                     /locus_tag="PKM49_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY82500.1"
                     /translation="MRRSFIRCFLCLILIFSCTVVWALPDYYTLWVPVDVEAQDPAAA
                     YRPIASDDRYEPFEDLTWDHPLRIAVSSDPHFGRAESNAEATKSIIRSVNETEPAFHA
                     FFVLGDITEFGFVDAHWLAAREAFAEFSRVPVVPLMGNHDDLMNGRSKFLRYLIPDAT
                     SETARQTLWQRIDIGPVHFFKLNLLWGVEDWGPDVEAWLEEELASIPREDWVVVLSHC
                     FFYSSGQYILGVPWFDHPETTTLIAPILERHRIDLSIAGHNHHMELATHHGVFYTVVG
                     AFGGVLSQTPFDHVSETSLWYRADTFGYADLTFYPDQVLLVFRDPEGVALKRFILPQN
                     LAP"
     gene            complement(4863..6662)
                     /locus_tag="PKM49_06515"
     CDS             complement(4863..6662)
                     /locus_tag="PKM49_06515"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HNY82501.1"
                     /translation="MKGRVVLAIFLTVLMIPVYGFLQLVFYNGYFDWFIDSKYSIESA
                     QIEQIVAADGSVEVHEMIHYRMRKPFKGLYRSIPVGRYVTLENVELWTEGAPTKRVEF
                     LQKSNQHFEARVWISDSDYTSLNPADYRDITLHVKYTAKNVLEIGRDADQIFRQFWGT
                     GWDSFAKDVTGVFFLPDSLRPERVYTHPNMSHTVERNVHTFHVDHIAPNAYAEVRFVF
                     QNAPNLPFAAENPTLTLSAIQSEERSYQTAYWFHWILRAALFLVFFWLLFFLYRKYGV
                     EHEITYQGIYERELPSDDSPDVINALIKNRAGSVDDDGIASAMMNLYRKDYLSFDQSK
                     KDLVIILNPQANKKIYKTEENLLAFLRKYATDDVFDFGQLRKTLKKSQKQAIDFTNRL
                     STYKRLVNKTAAQKDIFELKGATYSKSLALLFMVLSAGILIPFQTVKIPFFLGINTLL
                     FGVLWIISTLVLMLPKEVYGRWTKTGAEYYQKWRNFGRYISDISAIKDYPPESVAVWE
                     HYLVYATALGIADKVEKVLKSAIPKELWDEQSQHRQLYNLHSMGLLTQWSALRAVSAS
                     AASSKGGSGGGGGFGGGAGGSGGGSGGGGGGAF"
     gene            complement(7039..>7808)
                     /locus_tag="PKM49_06520"
     CDS             complement(7039..>7808)
                     /locus_tag="PKM49_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82502.1"
                     /translation="NIIATYTQEITQGEIYGTWAEYIVKVFLEVNERNAKRIMLYLFQ
                     AGEERTRAQMIKDLNLDMTDFDLQMKLLQLIKADLISLGSTAFRYAIAQDKTYELVFR
                     RLFQDEIDHFIPDIRKELCQEMGRTSYKKGKFREYLVRERIKKPFNLKDLAENGIDLT
                     IKPKTILERETVKIGLLDREIDLIVKGNVEMWIDVKDTKGKYGKREADRWIEIKQAIE
                     EKSPKTLFATYSQNGYKAMVKELLVSNGVYVLKGEEG"
BASE COUNT         1653 a         2245 c         2061 g         1849 t
ORIGIN      
        1 tgacgaaaga cgcggaggaa ttgctgacat tctatgattt tccagcagcg cacttccagc
       61 atatcagaac gacgaatccg atagaatcga gcttttcgac tatacggctc aggacaaaaa
      121 agatgaggaa ctgtggcaac cgaaaaacaa cgttagcgat gatgtacgaa ttatcccaac
      181 aggtggagaa ggggtggcgc aaactgcggg gattcaaaga gattcaatac gttctggaag
      241 ggaatcagta tctcaacggt tcaagaatga aaaatgtagt cgtttaacgg attgatggag
      301 gatacgctcg atttcgctta ttccacaaca tttgacaata actctggttt cagtttggct
      361 ctcccataag ttcaacgcgt ttattcccgg gcacaacttc ctcacccaaa gcttagcggt
      421 caaataactc gacccacctt ctattcacaa aacaataata ccatgataac gtcaggaaac
      481 gccaactttg tctcaccaac gaaaaagcaa cccgggcgcg ccctcggacc accttgggag
      541 cacccgggaa cgccctggga acgccaactt ttatgttggc atgtcttcta cgttttcata
      601 cctcctccgt gccctctatg taaacccttt ttttcttcta gggaaccatg ccgaactaaa
      661 gttcggcgtt cccagaaaac cttcctccgt ggtctctgtg ttctctgtgc gagaccttcc
      721 aatttccttt taaacgtttt ttgattgaaa aggcatgccg aactaaagtt cggcgttccc
      781 agaaaacctg cctcagtggt ctctgtgttc tctgtgcgga acctttttcc ttatgaatgt
      841 tttttaaatg aaaagatatg ccgcactaaa agtgcggcgt tctcagcgcg ttcccagccc
      901 gctgctctgg agccactccg aagacgttcc cagaaaaaaa tgcctcactg aagtccgccg
      961 ctccctccct cgcgaatatc ataataaatt aacgacattc ccgctataaa cgacatatcc
     1021 gtttggtaag atggactata gaaccaactg tcgttcaggg gagccgtgag gctgagaggg
     1081 attctattcc gacccttcaa cctgatccgg gtaatgccgg cgtagggaga atggacacaa
     1141 tcacgcgcat aacaccccga ccctgtcgac agacggggtt ttttattgca aaggaggcgg
     1201 ttctatgaac cgttatcgct actgtttggc catcctgctt ggctgtcttt gtgtgatagg
     1261 gttttcgggc cttcgcgtgt atgcctatga gagcttgaac tggctcaaag acacgctcat
     1321 cccaattttc gaggaacaaa ccggcatcga ggtcactctg gtgaccttag gcgatgccgg
     1381 caacatcctg tcgcgtctga ttctggaaaa gcggcgcccc ctttccgacg tggtcttggg
     1441 cttggatcaa agccttgcct accgcgcgct gtccgagggg cttctcgagc ccgtcgcccc
     1501 gcgaaacgcc gaccggatcg tcgacaaaag cctgtttttc gagcctgaag gatacctcta
     1561 cccattcgac tacggcgcgc tcgccatcat ctacgatccc gaccggcttc cggaccctcc
     1621 cacttccttc agagaactca cgacccgtcc caaaagctta ttgattcagg acccgcgaaa
     1681 ctcgagcacc gggctcgcct ttttcctgtg gaccgtcgcc gtctatcgag aagactggcg
     1741 agagtactgg aaagccctgc gaccagcgat cttgacgctc accaccggct ggagcgaagg
     1801 attcgcgaag ttcgaggcgg gggaggcccc gatgatggtg agttacgcca ccgacggcgc
     1861 ttactcttac cacgcctatg gctcagaccg atacaaggcc ttcataccga tggagggagc
     1921 cttctaccaa accgaatacg taggcgtcac caagggcacc gaaaatcccg agcaagcgcg
     1981 cctgttcgtc gaattcgtcc tctcagagcg cttccaaagc gaggtgccgc tcaaccaatg
     2041 gatgttcccg acgacccctg tcgagttgcc cgaagcttac gagcacgcgg tgacttcatc
     2101 cattcacgtg tcgatcagcg cggcagaagt ttcgaaaaac ctagaccagt ggttggccga
     2161 atgggaggag atcatgtatt aagcgcctag gccttcgggt cgccatcctg ctgttcgtcg
     2221 gattatgggc ggtcccgttc gtaatacttc tgaaggactt cctgcgccta gaggacctca
     2281 aactcctttt cgacacgaag accctccgga tcctggggtt caccctcgcc caagccgccg
     2341 gctcgacggc gctcgctctc gggattgcct tatgccccgc gtactacctc tatcgaagac
     2401 gcaacggact ctccgctcta ttggagagcg caatcttcat cccctttttc ctgccaccgg
     2461 tctcgacggt catcgccttt tcgctcctgt tctcgacgaa cggactcctg tcgcgtttgg
     2521 gcatccatat ccctatccta tacacgatgt gggcaatcct ggtcgcccac gccttctaca
     2581 actcgcctat attcgtcaaa tacataggcg ccgccctacg gaccattcct cccgccttgg
     2641 aagaggccgc gctgtgcgaa ggggcgggac ggtgggcgcg gttctggcgg atcgagggac
     2701 ccctcgttct ccccgcggcc gcgaaggcgt tctttttgtg cttcgcctac tcctttatga
     2761 gcttcgcggt cgtcctgaat ttgggcggtc tgcaatactc caccctggaa gtggcgatcg
     2821 ccggcgccct ccgtggtcag ttcgattttt ctcgcgcgct gacttacgcg atcgcgcaat
     2881 tcggctgtct tttcctcctc aacgcgctcc tctcgctgat cccggaagtt tccacgggcg
     2941 gcgcgccgct ttcgctctcc cgcgagcgcc cgctcagact cacgaccgta tcgagcgctc
     3001 tgtacgctgc atttgagtat gggatcgtac tgatcgggtt gctcggcctg tgttacgatt
     3061 tccggttggg tgccttctcc ctcgagccct tcctccggtt gttttcggcg gactttaacc
     3121 gtcggttccc ggtcatccgc tctttgatca acaccttcgg cgtggctagc ctcgcggcga
     3181 tcggggccgt cttagccgcc ttcctctttg gaaaggcgcg tggtaattgg gcgcagcgtg
     3241 tcatcttgcc cacgctgggt gtatcctccg ccttcctcgc gatggccctc ctctacctcc
     3301 acatcctcta tcgcctgccc ctctttctgt tgctgacgct gggctatctc ttgtccacgt
     3361 tggccgttgc ctacgcgttg atgcgcccga tggtccggaa ctttccactt cattgggtgg
     3421 aatcggcaaa aatcgacggc gcctcgcggc ttcaagtctt tcggtcgatc gagttaccgc
     3481 tgttgttccc cgtcttcgcg gccgtgttct tccaggtgtt cgccatcgtc ttcggagaat
     3541 ttaccctgcc ctacacaatg caaattcgcg actacctgcc cttggccagc gtggtgaact
     3601 atgcgatgga cgcgcaacga tactaccgag agagcgccgc tttctcgggg atgaacaccg
     3661 cgattgtagg cgccctcttt ctggtatcga acgcgctcat ccaacgccga cgtgtgatct
     3721 gagcgtcaac gaccaatggt gaagcgtgta gctttcgtta aggcgcaagg ttctggggaa
     3781 gaatgaatcg tttgagcgcc acgccttccg gatcccgaaa gacgaggagg acctgatccg
     3841 gatagaaggt caggtcggcg tacccgaagg tgtccgcccg ataccagagc gaagtttcgg
     3901 aaacgtggtc gaagggggtc tgtgacaaca cgcccccgaa ggcgcccact acggtataga
     3961 agacaccgtg atgggtagcc agttccatat ggtggttgtg gccggcgatc gataggtcga
     4021 tcctgtgtct ctccaagatg ggggcgatca gcgtcgtggt ttccggatgg tcaaaccaag
     4081 gtactccgag aatgtattga cctgaggaat aaaagaaaca gtgagacagg acgaccaccc
     4141 aatcttcgcg cgggatagac gcgagctcct cttccagcca agcctcgaca tccgggcccc
     4201 agtcctccac gccccacagg aggttgagct tgaaaaagtg gacgggccca atatcgatgc
     4261 gctgccacag cgtttgcctt gcggtttcgg acgtcgcgtc cggaatcagg tagcgcagga
     4321 acttcgagcg gccgttcatc agatcgtcgt gattgcccat aagcgggacg actgggactc
     4381 tcgaaaactc cgcgaaagct tctcgggcgg ccagccagtg ggcgtcgacg aatccgaact
     4441 cggtgatatc ccccaacaca aaaaacgcat ggaaagcggg ttcagtttca ttgacggagc
     4501 ggatgatcga tttggtcgct tcggcgttgc tttcggctct cccgaagtgc gggtccgaag
     4561 acacggcgat ccgcaggggg tgatcccaag tcaagtcctc gaacggctca taccggtcat
     4621 cggaagcgat cgggcggtag gccgccgccg ggtcctgcgc ctcgacgtca accggaaccc
     4681 acaacgtgta gtagtcgggc agcgcccaga ccaccgtgca cgaaaagatc aggatcaggc
     4741 ataaaaaaca gcgtatgaac gatcttcgca tcgcaatcct cctgaagggc cgggggcaat
     4801 ttatgggtgc gccggggcct tgtatcggga taatggaccg gaacgagaaa cggctcgagg
     4861 ccttagaaag cgccaccgcc tccgccgccg gacccgccgc cggacccgcc tgcgccgccg
     4921 ccgaagccgc cgccaccgcc agaaccccct ttgctgctgg ccgcggaggc cgaaaccgcc
     4981 cgcaaggcgg accattgggt caacaggccc atagagtgca aattgtagag ttggcggtgt
     5041 tggctctgct cgtcccagag ttccttcggg atcgcgctct tcagaacctt ctccacctta
     5101 tccgcgatac ccagagccgt cgcgtagacc aaatagtgct cccaaaccgc gaccgactcg
     5161 ggcggatagt ctttgattgc ggagatgtcc gagatatacc ggccaaagtt ccgccacttt
     5221 tggtaatact ccgcgcccgt cttcgtccat ctcccgtaca cctctttcgg aagcatcaag
     5281 accagggtgc tgatgatcca caacacaccg aagagaaggg tgttgatccc cagaaagaac
     5341 gggattttca ccgtttgaaa tggaatcagg ataccggcag acaggaccat aaacaacagg
     5401 gcgagcgact tcgaataggt cgccccctta agttcgaaga tatctttttg agctgccgtt
     5461 ttattcacta ggcgtttata ggttgacaag cggttggtga agtcgatcgc ctgcttctgc
     5521 gactttttaa gggtcttccg cagctggccg aaatcgaaaa catcatctgt cgcgtacttc
     5581 ctcaggaagg ctaaaaggtt ctcttcggtc ttatagattt ttttatttgc ctgcgggttg
     5641 aggatgatga cgaggtcctt cttggattgg tcgaaggaga ggtaatcctt gcgatagagg
     5701 ttcatcatcg cggaggcgat tccgtcgtcg tcaacgctcc ccgcccggtt tttgatcagc
     5761 gcgttgatca cgtccggaga gtcgtcgctc gggagttcac gttcatagat gccttggtaa
     5821 gtgatctcgt gttccacccc gtacttgcga tagaggaaga acagaagcca gaagaagacg
     5881 aggaacaggg cggctcgaag aatccagtga aaccaataag cggtctggta cgacctttct
     5941 tcggactgga tcgcggagag ggtcaaagtg gggttctcgg ccgcgaaggg caaattcggc
     6001 gcgttttgga agacgaatcg cacctcggcg taggcattag gcgcgatgtg atcgacgtgg
     6061 aaggtgtgta cattcctctc cacggtgtgt gacatgttcg ggtgggtgta gacccgttcg
     6121 ggcctaagag agtccgggag gaaaaagacg ccggtcacgt ccttggcgaa agagtcccaa
     6181 ccggtgcccc agaactgccg gaaaatctgg tcggcgtccc ggccgatctc cagaacgttc
     6241 ttggcggtat acttgacgtg cagggtgatg tcgcggtagt ccgccggatt cagggaggtg
     6301 taatcgctat cggaaatcca cacccgggcc tcgaagtgct ggttgctctt ctgcaagaac
     6361 tcgaccctct tcgtgggggc gccttcggtc cagagctcga cgttctcgag cgtcacataa
     6421 cgtcccacgg ggatactgcg gtacagacct ttgaatggct tacgcatccg atagtggatc
     6481 atctcgtgca cctccaccga cccatcagcg gcaacgatct gctcgatctg ggccgactct
     6541 atcgaatact tgctatctat gaaccaatcg aagtacccgt tataaaagac cagctgcaag
     6601 aacccataaa ccgggatcat caacaccgtt aggaaaatcg ccaacaccac tcgccctttc
     6661 aacgcgacca ccctctttgc tcagtttccc ggattataac acaaagaaat cgcatggccg
     6721 ggagcgccca gggaacgccg cactttagtg cggtatgtct tgcttttctg gaaacgcctg
     6781 gaaacgctta aacgctggga atgccaactt ttaagttggc acttcctgaa aaaaggggcg
     6841 cgtggagggc acagaggacg cggagaaggg aggcactcta aagagcggca tcgcggcgta
     6901 agcgcttctt ctttttgcga cggcgcgata ccatttttag aaagacacgg agcgaatcgc
     6961 ggggtcctgt tagttacccc gcgatgaacc acaaagttgg cgttcccagt gctcctaggc
     7021 gttccctaag cgcttctact acccctcttc ccccttaagc acgtacaccc cattcgacac
     7081 gaggagctct tttaccatgg ccttgtaacc attttgtgag tacgtcgcga ataaagtttt
     7141 agggcttttt tcttcgattg cctgcttgat ctctatccag cggtccgctt cgcgtttccc
     7201 gtacttccct ttcgtgtctt tcacgtcgat ccacatttcg acgttgcctt tcacgatcag
     7261 gtcgatctct cggtccagta acccgatttt gaccgtttct cgctctaaga tcgttttcgg
     7321 tttgatggtg agatcgatcc cgttttccgc gaggtctttc aggttgaacg gcttcttgat
     7381 ccgttctcgt acaaggtatt ctcgaaattt tcccttcttg tagctcgtcc tccccatctc
     7441 ttggcagagc tcttttcgga tgtcagggat gaagtggtct atttcgtcct gaaacagtct
     7501 cctaaacaca agttcatagg tcttatcttg cgcgatggca tatctgaatg cagtgctacc
     7561 caatgagatc aggtccgctt tgatcaactg gagcagtttc atctgtagat caaagtcggt
     7621 catatcgagg ttcaggtctt tgatcatctg agcgcgcgtg cgttcttcac ctgcctgaaa
     7681 taggtacagc ataatccgtt tcgcgtttct ttcgttcact tccaagaaga ccttcacgat
     7741 gtattcggcc caggtgccgt agatttctcc ttgagtaatc tcctgtgtgt acgtcgcgat
     7801 gatgttgt
//
LOCUS       DAOPXN010000099         7778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9347_length_7778_cov_5.884112, whole genome shotgun sequence.
ACCESSION   DAOPXN010000099 DAOPXN010000000
VERSION     DAOPXN010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9347_length_7778_cov_5.884112"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..701
                     /locus_tag="PKM49_06525"
     CDS             57..701
                     /locus_tag="PKM49_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82503.1"
                     /translation="MKKVPILFFILILVLAALTLASSISLKFTDAYLVYVPSSQILQI
                     IAHDKVISYGSEWSVQQVRPYLYHIKLNMWQGFFWKVNTSQKKVFRTTDGEFGAIGGN
                     DTQMNVSLEVVGGSADVPPTRFAIRFNDAYLIYNIETQSIQIGAQQTALSYGTDWNKA
                     QVYPYLFHIRLATWKDFYWQVNTSRKELVEVTNGSFGKISGGTSTKIPIVVNVQ"
     gene            complement(720..1340)
                     /locus_tag="PKM49_06530"
     CDS             complement(720..1340)
                     /locus_tag="PKM49_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNY82504.1"
                     /translation="MLAQDKIKRVFCIGRNYRLHAQELNNVVPERPVVFLKPRESILF
                     EGSEIIHPAPGRDLQFETEWVVRIGKEGLYKEGETAADWIEGMTLGLDLTLRDLQESL
                     KKKGLPWEKAKAFDGSAPLGAFVPYVPEKLTFTCHVNDRLRQTGNTELMIFSIEACLA
                     EISRYWRLLPGDIMYTGTPEGVGSLASGDRVTIASASIGAFSWFVR"
     gene            complement(1389..2030)
                     /locus_tag="PKM49_06535"
     CDS             complement(1389..2030)
                     /locus_tag="PKM49_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNY82505.1"
                     /translation="MREERFSSHEEIAHAISHGAGVLLGVTALTLLTVFSALARDPIR
                     VFSSIVYGVSMILLYLSSTLYHGFKKEKIKNLFEIFDHSSIYLLIAGTYTPFLLTVFP
                     GLTGWILFGVIWGIALFGVVFKVFFVKRFVVFSTILYIAMGWFIVFALRAVFNALSHR
                     GFFFLLFGGILYTVGTFFYLYRRFRYHHLVWHLFVLAGSLFHFFAILFDIILR"
     gene            complement(2032..2754)
                     /locus_tag="PKM49_06540"
     CDS             complement(2032..2754)
                     /locus_tag="PKM49_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82506.1"
                     /translation="MRRISTYNESDLHDALKEYFRGTDGIKEASIKGYSVDVLRDGRI
                     YEIQTASFGALKQKLSMLAPDWPISVVYPIPEVKWIIRIDEDGGVISRRRSPKKGSGY
                     EVFKELVYLAPLLPDPAITIVLAFVDVEEERRQDGKGSWRRKGDSVVSRKLLSFRTTQ
                     TLSDPEEYRLFLRLSESSPFTTADIRKNLSVSASLASRIAYFLKTTGMAEFIGKTGRM
                     HLYRWRVETKESGLYRQWRTEN"
     gene            complement(2732..3832)
                     /locus_tag="PKM49_06545"
     CDS             complement(2732..3832)
                     /locus_tag="PKM49_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HNY82507.1"
                     /translation="MNYIDLRSDTVTEPTEEMREAMKTAPVGDDVYADDSTVNRLEEM
                     AARELGKEAALFVPSGTFGNQLSILTHTLRGDEVLIPEDNHIVIHEAGASAVIAGVQL
                     RFVPATRGIIQPEELESRIREDDLHTPRTGLICVENAHSCGAVVPLDNMAEVYAVGQK
                     AGIPVHLDGARIFNAAAALGCPAKEIAQYADSVMFCLSKGLCAPIGSIVAGTEYFIRR
                     ARKNRKIMGGALRQAGVIAAPGIIALERMRGRLAEDHRRAKALAAGLSKLKGVTVFSE
                     QLDINMVFFTIDPGVDKRDRARYREAGDQLARFLTKELLDRKIKINGPEGQVYRFATH
                     YWISDDDIAKVLTCLESALADFTRKNAQNLHL"
     gene            complement(3850..5133)
                     /locus_tag="PKM49_06550"
     CDS             complement(3850..5133)
                     /locus_tag="PKM49_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82508.1"
                     /translation="MDPLNETEGISCFSRERVWALVSAFAEMIKKPYAESANLLLDLL
                     KSGPDEREVEGDSKSMYEALRAFEQRERGAGWPPLFSEPKGTENPDQEGPALSFFGQY
                     VYRVLREWAKAGDLRLLAEMTRNAWYSQNEAAMAEAVAAFSTALQVQERAFSRRVAEI
                     EKGRRPAPFRKLIEVADRLSKELLESLRILKTSIEEGAPGEEVSTHTDQRPGAPHRGK
                     ITDLIDAAERFLRKTLSIVDVYETLWEEECDRLFSSKPRVIESQLLPALLAPYPSSSS
                     QEAYHSFFRLLIPPAIPQRVCLATRLTHRGDTSIKKETALRKKREKTPFDSAVLLFFL
                     TLLKQVRIGKTTLSDIMQKIPITEELPVLFMRLHLSHDIWFHRCGFRELEEALRRKRM
                     KNDYLGIRIETTDSLVTIGQFRMNDLSFRLMGRRG"
     gene            complement(5121..6266)
                     /locus_tag="PKM49_06555"
     CDS             complement(5121..6266)
                     /locus_tag="PKM49_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HNY82509.1"
                     /translation="MSLAAIILSLLTLIVLHRKTKRLLLAIVASILVLICFRFDLAAL
                     GWSLLDPFTDPSFYKLVVTLFLIYYFSHMLAFSGDARTFTHSSQALFDNPTAIAFMPT
                     LIGFLPMPGGAMFTAPMIRNLGEPEGKSPDYLLAANYWFRHTVEFFWPVYTGMYLIVS
                     LTGQNLAAFSVKLFPVFLSGFISGWFFLNGFRLPRVKKAPLKELKGLWLVGFIVLIGV
                     LILGLNIEGYLALLICIGAYSVLRRKHISPALKGALQKWDVYVLLYLFYVYKSYLIQV
                     DFPASLAADFQALNLGVGWMTALLPFFIGIATGLTPSTIGITTPLLMKLGADATWLYF
                     WAVVGVVLSPVHLCSVLTADYFKADMARMFLRILPMIAISGLVLWIL"
     gene            complement(6273..6803)
                     /locus_tag="PKM49_06560"
     CDS             complement(6273..6803)
                     /locus_tag="PKM49_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HNY82510.1"
                     /translation="MRIAVIGYSGKIDQSPVRELRDLCLALGKQIARQGHSLWSGGRD
                     GVMALTSMGAASENGEVFGVLPYDEQEEIVQPCPYVKYPIYTGLDFQMRSCVLLKNAD
                     LVISIGGACGTAIEIFGAYGYGKPLVLMKNTGGVTERIAGWFDRSAPPFYLDYRKSAP
                     VYIETNVEELTKYYAR"
     gene            complement(6835..>7778)
                     /locus_tag="PKM49_06565"
     CDS             complement(6835..>7778)
                     /locus_tag="PKM49_06565"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNY82511.1"
                     /translation="FVGHRFSRYYQIPQALLPISQTPFLVDNSLGLPGDPEELKRYLP
                     ELYLRERKSVEQTLGVYLQVAGEVQGLITLDIARGRRTTFSEAHKEIIHSFANIVSSF
                     LAIRTFAHQSEGFQKEIILSFIKILELYDPYTQGHSEAVALLSSMIAEELRIDATDFK
                     RVYWAGLVHDIGKILVPSDILKKKSALTKEEYTVIQKHPQWGAEVLHSSEKLKEIAEY
                     VLFHHERWDGTGYPIGLSGEEIPYISRIISIADALDAMTSDRPYRKGMGVTIAFQELE
                     RGAGSQFDPDLVRKILGQPACRERVAGIIQNKRIGGI"
BASE COUNT         1839 a         2131 c         1990 g         1818 t
ORIGIN      
        1 tttcattcag tttttcgctt atttggtttt tgtaatgacc acgcacatgt tgagttatga
       61 aaaaagttcc gattctattt ttcattctca tcttggttct tgcagctctc accttggcaa
      121 gctctatcag cttgaagttc acggacgcgt atctggttta tgtgccttcg agtcaaattc
      181 tccagatcat cgcgcacgat aaagtgatct cgtatggttc cgaatggagc gttcagcagg
      241 tacgacccta tttgtatcac atcaaactca atatgtggca aggcttcttt tggaaggtca
      301 atacctctca aaaaaaagtg tttagaacaa ccgacggaga gttcggtgct attggcggca
      361 atgacaccca gatgaacgtt tcgctggagg tggtcggcgg ttcggccgac gtcccgccga
      421 cgagatttgc gattcgattc aacgacgctt acctcatcta taacatcgag actcaatcga
      481 tccaaatcgg cgcccagcaa acggcgcttt cctatgggac tgattggaac aaagcccagg
      541 tctatcccta tttgttccat atccgcttag cgacctggaa agacttttat tggcaggtga
      601 ataccagtag aaaagaactc gtggaggtaa cgaacgggtc attcggcaag atctccggcg
      661 ggacgtctac caagataccg attgtcgtta acgtccagta gccagcgtac atcgcgtagt
      721 taccggacga accagctaaa ggcgccaata gaagcagatg caatcgtgac ccggtctccg
      781 gatgccaacg acccgacgcc ctcgggtgtg cccgtgtaca tgatatcccc gggcaacaac
      841 cgccaataac gggatatctc cgccaagcac gcttctatcg agaagatcat caactcggtg
      901 tttcccgtct gccttagcct atcgttcacg tggcacgtga aggtgagctt ctctggaacg
      961 tatgggacga aggcgcccaa gggggcgctc ccgtcgaacg ctttcgcttt ttcccaaggg
     1021 agcccctttt tcttaagcga ctcctgcaga tcgcgaaggg tgaggtccag ccccagcgtc
     1081 ataccctcga tccaatccgc cgctgtctct ccttccttgt acaacccttc ttttcctatg
     1141 cggaccaccc actccgtttc aaactgcaaa tcccgcccgg gagctgggtg gatgatctca
     1201 cttccctcga aaagaatcga ctctcggggt ttcaggaaca cgaccggtct ttcgggtacg
     1261 acgttgttta actcttgagc gtgtagcctg taatttcttc cgatacagaa tactcttttg
     1321 attttgtctt gcgccagcat ctctctccct ttcttttcgc tcgtttgttg atgatgtgag
     1381 ggtttccgtt atcgcaggat gatatcgaac agaatggcga aaaaatgaaa caggctcccg
     1441 gcgaggacaa acagatgcca gaccaggtga tgatagcgga acctgcgata aaggtagaaa
     1501 aacgtcccga ccgtatacag aatcccgcca aacagtaaaa agaagaatcc tcggtggctc
     1561 agggcattga aaacggctcg aagggcaaag acgatgaacc atcccatcgc gatatacaaa
     1621 atcgtcgaaa agaccacgaa tcgcttgaca aaaaacactt tgaaaacaac gccaaacaac
     1681 gcgattcccc aaatcacgcc gaaaagaatc caaccggtca atccggggaa caccgtcaat
     1741 aaaaacggcg tgtaagtccc cgcaatcagc aggtaaatcg aggaatggtc gaatatttcg
     1801 aacaggttct tgattttttc ttttttgaac ccgtgataca gggtggaact caagtaaagg
     1861 aggatcatag atacgccgta gacgatgctg ctgaatactc ttatcggatc gcgcgctaaa
     1921 gcggaaaaca ccgtcagtag cgtgagcgcg gtgactccaa gcaatacgcc cgcgccgtga
     1981 gatatggcgt gggctatctc ctcatgcgag gaaaacctct cttctctcat ttcagttttc
     2041 tgttctccat tgacggtata ggccactttc ctttgtttct acgcgccacc ggtacagatg
     2101 cattctcccc gttttcccga taaactccgc catcccggtt gtttttagga aataggcgat
     2161 acgcgaagcc aaagacgcgg ataccgagag gtttttccgg atatcggcag ttgtgaaggg
     2221 gctactctca ctcagccgta ggaataaccg gtactcttcc gggtctgaca acgtctgtgt
     2281 tgttcgaaaa gaaaggagct tacggctgac cacgctatcg ccttttcttc tccagctccc
     2341 tttcccatcc tgccggcgct cttcttccac atccacgaag gccaagacta tcgtgatcgc
     2401 tggatccggg aggaggggcg ccaaatagac cagctcctta aagacctcgt aaccgcttcc
     2461 cttttttggc gaccgcctgc gagatatgac cccaccgtcc tcgtcgatcc gaataatcca
     2521 tttgacctcc ggtatcggat aaacgacgga gatcggccag tctggcgcca gcatcgagag
     2581 tttctgcttc aacgccccga agctcgcggt ttggatttcg taaatccgcc catctcgtag
     2641 gacgtcgacc gaatacccct ttatcgatgc ctccttgatc ccgtctgtcc ctctaaaata
     2701 ctccttaagg gcgtcgtgca ggtcgctttc gttataggtg gagattctgc gcattcttcc
     2761 gggtgaaatc ggcaagcgcg ctttctaggc acgtgaggac cttcgcgata tcgtcgtccg
     2821 aaatccagta atgcgtggcg aaccggtaga cctgcccttc cggaccattg atcttgatct
     2881 tccggtcgag aagctctttc gtcagaaacc gagcgagctg atcgcccgcc tctcgataac
     2941 gcgctctatc ccgcttgtcg acccctgggt cgatcgtaaa gaacaccata ttgatgtcca
     3001 gctgttcgga gaagaccgtt acccctttga gtttcgatag accggcagct aaggctttag
     3061 ccctccgatg gtcttctgct agccgacctc tcatccgctc tagcgcgata atgcctggcg
     3121 ccgcaatcac accggcttgg cgtagtgccc cgcccataat cttccggttt ttcctggccc
     3181 tgcggatgaa gtattcggtt ccggcgacga tagacccgat cggcgcgcag agccctttgg
     3241 aaagacaaaa cataaccgaa tccgcgtact gagcgatctc tttcgccggg caccccaacg
     3301 cggcggcggc attgaaaatg cgcgcgccgt ctaaatgcac cggtataccg gccttctgac
     3361 cgacggcgta gacttcggcc atattgtcca aaggaaccac ggcgccacag gaatgggcgt
     3421 tttccacgca gatcaacccc gttctcggcg tgtgcaaatc gtcctcgcgg atgcgcgact
     3481 cgagctcttc cggttggatg atccctcgtg tcgcggggac gaagcgcaat tgcacgcccg
     3541 cgataactgc cgaggcgcct gcctcgtgga tgacgatatg gttatcctcc gggatcaaca
     3601 cctcgtcgcc gcggagggtg tgggtcaaga tcgacagctg attgccgaag gtcccagagg
     3661 gaacgaagag cgcggcctct ttacccagct ctcgcgcggc catctcttcc aggcgattga
     3721 ccgtagaatc atcggcgtaa acgtcatcgc cgacgggggc ggttttcatc gcttcccgca
     3781 tctcttctgt cggttcggtg acggtgtcgc ttcttaagtc aatgtaattc aaaggtattc
     3841 aactcctcct caacctcgcc gacccatcag tcggaacgag agatcgttca tacgaaactg
     3901 tccgatcgtg acgagagaat cggtcgtttc gattcttatt ccaagataat cgttcttcat
     3961 acgtttgcga cggagcgcct cctctagctc acgaaaacca caacgatgaa accatatgtc
     4021 gtgagacagg tgcagcctca taaagagaac gggcaactcc tccgtaatgg gtatcttttg
     4081 cataatatca gagagggtcg ttttcccgat tcgaacctgc tttaacagcg tcaggaagaa
     4141 taacaagacc gcggaatcga agggggtttt ctctcgcttt tttcgaagcg cggtctcttt
     4201 ttttatggac gtatccccgc gatgggtcaa acgtgtcgcc aagcagaccc tttggggtat
     4261 cgccggaggg atcagtagac ggaaaaaact gtgataggcc tcttgcgaag aactagaggg
     4321 gtaaggcgca agcaaggcgg ggagcagttg gctctcgatt actcggggtt tgctagagaa
     4381 aagccggtcg cattcctctt cccagagcgt ttcgtaaaca tccacgatag agagggtctt
     4441 tctcaaaaaa cgctccgccg cgtcgatgag gtcggtgatt tttccacgat ggggcgcgcc
     4501 tggtcgttga tctgtatggg ttgaaacctc ttcgccggga gcgccttctt cgatggacgt
     4561 tttcaggatg cggaggcttt ccaaaagctc cttcgaaagg cggtccgcga cttcgatcag
     4621 tttgcggaat ggggccggtc gcctgccttt ttcgatttca gccacccgcc tagagaaggc
     4681 gcgctcttgg acttgaagcg ccgttgaaaa cgccgctacc gcttccgcca tcgcggcttc
     4741 gttttggctg taccacgcgt ttctcgtcat ctccgccaag agtcgcaaat ctcccgcctt
     4801 tgcccattct ctcaacacac ggtagacgta ttggccaaaa aaagagagtg cgggtccttc
     4861 ctgatcggga ttttctgttc ctttaggctc ggaaaaaagc gggggccaac cggcgcctcg
     4921 ttcacgctgc tcaaacgctc taagcgcttc gtacatcgat ttagaatccc cttctacctc
     4981 ccgttcgtcg ggtccgcttt taagaaggtc caagaggagg ttcgcgcttt cggcgtacgg
     5041 cttcttgatc atttccgcaa aagcggagac gagggcccag acgcgctcac gggaaaaaca
     5101 ggagatcccc tccgtttcgt ttagaggatc catagcacca gtccagagat cgcgatcatc
     5161 ggtagaatgc gcaggaacat ccgtgccata tccgccttga aataatcggc tgtaagcacg
     5221 gagcagagat gtaccgggga gagaaccaca ccgaccaccg cccaaaagta cagccaggtc
     5281 gcatcggcgc ccaatttcat taaaagcggc gtcgtgatgc cgatcgtgct tggcgtgagc
     5341 cccgtcgcaa ttccgataaa aaagggtagt aaagcggtca tccagcctac gcccagattc
     5401 agcgcctgaa aatctgccgc caaggaagcg gggaaatcca cttggatgag gtagctttta
     5461 tacacgtaga aaagatagag taggacatag acgtcccatt tctgcagggc ccccttcagc
     5521 gcaggagaaa tgtgttttcg gcgaaggaca gagtaggccc ctatacagat caacaaggcc
     5581 aaataccctt cgatgttcag gcctaagatg agaaccccga tcaatacgat gaatccgacc
     5641 agccataacc ctttgagctc tttgagcggc gctttcttga cgcgtggcaa tcgaaagccg
     5701 ttcaagaaga accacccgct gatgaatccg gacaaaaaaa ctggaaacag cttcaccgag
     5761 aaagcggcca agttctgacc ggttagcgag acgatgaggt acatcccggt gtaaacgggc
     5821 cagaagaact cgaccgtgtg tcggaaccag tagttcgcgg cgagaaggta gtccgggctt
     5881 ttcccttcgg gctcccctaa attacggatc attggggcgg taaacatcgc cccgcctggc
     5941 atcggcagaa acccgatcaa agtcggcata aacgcgatcg ccgtcgggtt gtcgaacaag
     6001 gcttgagagg aatgggtgaa ggttcgcgca tctccagaaa aggccagcat atggctgaag
     6061 tagtaaatta agaatagggt gacgacgagc ttgtagaagg atggatcggt aaaggggtcc
     6121 aacaggctcc aacccaatgc ggccaagtca aaccgaaagc aaatcaaaac gaggatcgaa
     6181 gccacaattg ccagaagcag gcgcttggtc ttccggtgta aaacaatcaa cgtcaataac
     6241 gagagtatga tagcggctaa agacacatag ggtcacctgg catagtattt tgtgagttct
     6301 tcgacgttcg tttctatata tacgggtgcc gatttcctgt aatccagata aaacggaggg
     6361 gcgctccggt cgaaccaacc ggcgattctt tctgtgaccc caccggtgtt cttcatcaga
     6421 accaacggtt ttccgtagcc ataagcgccg aaaatctcga tggccgtccc acaagcgccg
     6481 ccaatcgaga tgaccagatc cgcgttcttc aatagaacgc agctacgcat ttgaaagtct
     6541 aagcccgtgt agatcgggta cttgacgtag ggacaaggtt gaacgatctc ctcttgctcg
     6601 tcgtagggaa ggactccgaa tacctccccg ttttctgaag cggcgcccat cgaggttaag
     6661 gccatcacgc cgtcccgccc cccgctccaa agagagtgcc cctgtcgggc gatttgtttt
     6721 cctagcgcca ggcagaggtc cctcagttcc ctgacgggag actgatcgat ctttcctgaa
     6781 tatccgatta cggctatgcg catcgctttc ctccccgttt cctacacata ggtctcatat
     6841 tcctccaatt cgcttgttct ggataattcc cgcaaccctt tccctgcacg ccggctgacc
     6901 cagaatcttt ctcaccaaat cgggatcgaa ttgcgaaccg gcgccgcgct ccaactcttg
     6961 gaaggcgatt gtcacaccca tcccttttcg ataggggcga tcggaagtca tcgcatccaa
     7021 cgcgtccgcg atggagatga ttctggaaat atacggaatc tcctccccgg ataggccaat
     7081 cgggtagccc gtcccatccc aacgctcatg gtggaacagc acgtattcag cgatttcttt
     7141 gagtttttcc gaggagtgaa gcacctccgc gccccattgc ggatgcttct gtatgaccgt
     7201 gtattcctct ttggtgaggg cacttttttt cttaaggatg tccgaaggca ccagaatttt
     7261 gccgatatcg tggactaatc cagcccaata aaccctttta aagtcggtgg catcgatgcg
     7321 caactcttct gcgatcatgg aggaaagcag ggcgaccgct tcggaatgcc cctgcgtgta
     7381 ggggtcatag agctccagta ttttgatgaa agagaggatg atctcttttt gaaatccttc
     7441 ggactgatga gcgaaggtac ggatcgccaa gaaagaagag acgatgttgg cgaacgagtg
     7501 gattatttct ttatgtgcct ctgagaaagt cgttcgacgg cccctggcga tatcgagggt
     7561 gatcaaaccc tggacctcgc ccgcgacttg gaggtagacg cccaacgttt gttcgacaga
     7621 tttgcgctct ctcaggtata attcgggcaa gtaccgtttc aactcttccg ggtctcccgg
     7681 taatcccagc gaattgtcga cgagaaaggg ggtttgggag ataggcagta gcgcttgagg
     7741 tatctggtag taccgtgaga agcggtgtcc tacgaaac
//
LOCUS       DAOPXN010000100         7715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9471_length_7715_cov_5.259530, whole genome shotgun sequence.
ACCESSION   DAOPXN010000100 DAOPXN010000000
VERSION     DAOPXN010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9471_length_7715_cov_5.259530"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            707..2899
                     /locus_tag="PKM49_06570"
     CDS             707..2899
                     /locus_tag="PKM49_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HNY82512.1"
                     /translation="MPETINGLSLTQKSLGFDEMLDILSKELGVETERVRNAVYLLDE
                     EKTIAFIARYRKEATQSLNEIQIRQISERIQYLRRLKERKEAVENAIREQGKWQEELD
                     RQIAAAMTLQEVENLYFPFKKHKKTKADVAREKGLAPLSDLIKEAKEPLDNEISAYLS
                     EENGLLRIEDVMQGVKDILREEITFSSEVRTRLYELLLQKGIVETEQLQAPDPKGLYR
                     DFYRSSKSVRSLEDHRILAMNRGEKAKVIRVKLSLPEDPFPRFFQIFRFSGSLHYYDT
                     LYQSYKEGFEELLFPSVVRQVRNALTEKAQTRAIEVFAHNLRHLLLVPPLRKRSVLGI
                     DPGLRTGCKCAVIDSNGMFLDHATIYPHAPHHAKSESEKILSELYERHPFEIVAIGNG
                     TASRETEAFVADWIAKRNHDVSYLIVSEAGASVYSASENAVEEFPELDVTSRGAISIA
                     RRVQDPLAELVKIPPESIGVGMYQHDLPVSELNRVLKMEVESVVNYVGVDLNQASPYL
                     LQYVSGLNRSKAWKIHEYKTEFGFFRSREDLKNVKGIGEKTYELAAGFCRIPESENPL
                     DNTVIHPESYERIHRLLKRVGSAFEEIGLRPDDFLKKVRSIGHQTLSTELQVTEGELK
                     DYLDALTIRHLDPRDSLPQPLLKKEVRGLDDLGEGMELEGTVRNVVDFGAFVDIGVEI
                     DGLIHQSQFGKRWVKPSAIVHVGEIIRVRILKVDKDRGRINLAFVQKA"
     gene            2952..4148
                     /locus_tag="PKM49_06575"
     CDS             2952..4148
                     /locus_tag="PKM49_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HNY82513.1"
                     /translation="MQDVRFFSALSKQYAEGKLNKVELETTLFYPDSEGGQLGDRGKI
                     GNADVLKVYRVDGGALVHEVSEPVTDTGRPIECLINGSRRLDISQQHTAQHLLSADIF
                     SLYGYETVGFAMGEAYSSIDIDGDQWDWERSESAERMMLEQIMAKVDVEILEVPKNDI
                     HLYPLRKKMSEKIEQLGTYRIVKIGHIDYSLCGGFHVSNLAQLGTVKIIKYEKVKSDW
                     TRLYYLAGFRALIDYQKKTRLLHETTRSLSTSVDELNYRIHNILTEHQEAKRINRKLS
                     ENLASALYNSFRSQKATDNVKTVVEILPSEEIAKGLSGKLLHEQNTISFLLYERPKGN
                     GYILSSTQENWNAQSIFRKVKEFLEIKGGGTEKMIQGTVEGNITAVKEKCESIIKSYF
                     SSGGLL"
     gene            4145..4633
                     /locus_tag="PKM49_06580"
     CDS             4145..4633
                     /locus_tag="PKM49_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82514.1"
                     /translation="MKKYVILGVILLVLPLSTFALQVGIAFSGDLQDATPCTYLGATV
                     RSSWGLAGLELSVMPQILPELDFTSYRFIISPSIGWGTPELRIFAGIAPHLMLKKGQF
                     DFDIAKWNIGAGASFMFAKNVICFFDFFVGFDFRNYEQIDKLTTGSFITVGVTYNFNV
                     GF"
     gene            4649..4885
                     /gene="hfq"
                     /locus_tag="PKM49_06585"
     CDS             4649..4885
                     /gene="hfq"
                     /locus_tag="PKM49_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNY82515.1"
                     /translation="MAEKYNLQDPYTNLARRKKEKIRIFLVNGFQVQGKLKSFDNFTY
                     LVEDGKTQSLIFKHATSTILPPSPIKLPPPKSEK"
     gene            5550..>7715
                     /locus_tag="PKM49_06590"
     CDS             5550..>7715
                     /locus_tag="PKM49_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82516.1"
                     /translation="MRAVGIIVGFLFVLTAFGSFQKIETEFFNILYEYGLEEQAEILF
                     REGPEIYRRLTDFYRIYPAKKLNVYLLDNNDYGNAYADFFSHAIVIYVNRAGASYINN
                     TYPLWVPFVFSHELTHILIANKPFWFKNFLNIFGRPAAMFFDTAFSSSYLHEGLSIYS
                     ESMLSGEGRFFDTQFDMYLRRDLYESRFYGFSLAGGPSSPIFNPVGMNYLYGASFFEF
                     LGERYGAKVVSSLLNTMGDQWMSLKRALETATMRPYSALLAEWESWCREKAYALLPKS
                     PIDAPENLTGTGFFTGHPIAIGSYLYYLSHSERGSEIVRRKGDGSEERWEVPYPSDYD
                     VSPNGRLAFVYAIGDGLELYTRYLYFGDWGVELWKSPLIERVIALRWMDDRWLWVTAV
                     ENGGTALWLVDTETGKKQRMISGSPHFYLNSLCVLSDITESPKAIVCSISYDGQARLY
                     RMPLMIDERAETYEFGNWEVVGSPKGNSLDPAYADGALYYANDLSGIFNLYRWEEESG
                     LIQQLTNVAVGAFQPALFDGELYYSEYSRNGFDLFRAPNSETPATLSLEGSMTFSPIP
                     TRYEALRVEMSPAQAIQPIPQPQTVVLWPKPRIWLPIVTLTPGGFGLFGGMVGWDDLK
                     RWVWSFNGYYLSESELMNTLDLSPWSIGVNLVYRGLIPFSASLLLNEEGFVGRVQLDK
                     SFVDRVNRDLIRMQPAIGIQWTLSDRSLWALQWEYYQAVG"
BASE COUNT         1903 a         1991 c         2079 g         1742 t
ORIGIN      
        1 ccctctttag aagggcgaag aagggatcgc ggggtccctt cggttacccc gcgataacac
       61 acgcgagcat cgcggcgtaa gcgccccttc ttttcgcgac ggctcgatac ccctttcatt
      121 cgtttccaaa gatcaaaaaa gggttcgcac agagggcaca gagaacacgg agagaacagg
      181 catcgcggcg taagcgctcc ttcttttcgc gacggcgcga tacgctattt agaaggatga
      241 agaagggatc gcgaaacttc gtgggcttcc gaagtcagcc cgctggttac cccgcgataa
      301 cacatacgag catcgcggcg taagcgcttt ttcttttcgc gacggcgcga taccccggtt
      361 ttcgttcgtt ctcaaaatca aaaaacaggg ttcgcacaga ggacacagag gacacagaga
      421 acacagagaa aagattgaag ataatttgta gggccggctt ccagccggcc tttctctggg
      481 tacgcctggg aacgccaaat ttcaagttgg ggggttttta gcccggccga aaaaaaccac
      541 ttaccccgcg agaatcttcg ttcttttccc acctgaaccc tatttcccga ctaaagacaa
      601 gaaaataccc cggtaagcca aggtataacg cggcgagtcg gtggaaaacg aaggccgtct
      661 ttgatacaat aatctcggta gatttttgta ggtgaggagt gatacgatgc cggagacgat
      721 aaacggatta tccttgaccc agaagtcctt agggttcgac gagatgttgg acatcctgtc
      781 gaaagagctc ggggtagaga ctgaacgagt gcggaacgcg gtgtacctat tagatgagga
      841 aaagaccatc gccttcatcg cgcggtatag aaaagaggct acccaatctc tcaacgagat
      901 acagatacgc caaatctctg aacgcataca atacctcaga cggctcaaag agcgtaagga
      961 ggccgtagaa aacgccattc gggaacaggg caaatggcag gaggagctcg atcgccagat
     1021 cgccgcggcg atgaccttgc aagaagtgga aaacctctac ttccccttca agaaacacaa
     1081 gaaaaccaaa gcggatgttg cgcgcgaaaa ggggcttgcc ccgctgagcg acttgattaa
     1141 ggaggcgaaa gagcccctcg acaacgagat ttcggcgtat ttgagcgagg agaacggctt
     1201 gctccgtatc gaagatgtga tgcagggcgt gaaagacatc cttcgagaag agatcacgtt
     1261 ttcatccgaa gtgaggacgc gcctttacga gctcctcctt caaaaaggta tcgtcgagac
     1321 cgaacagctc caggcgccgg acccgaaagg gttatacagg gacttctacc gctcgagcaa
     1381 gtcggtcaga tcgttggaag accatcggat tctggcgatg aatcggggag aaaaggcgaa
     1441 ggtcatccgc gtcaagctct cattaccgga agacccgttc ccacgatttt tccaaatctt
     1501 ccgcttctcc ggttctcttc actactacga cacgctctac caatcctaca aggaagggtt
     1561 tgaggagctt ctttttcctt ccgtcgttcg ccaggttcgc aacgccttga ccgaaaaagc
     1621 gcagacgcgc gcgatcgaag ttttcgctca caatctgcgt cacctactac tggttcctcc
     1681 actgcggaaa cggtcggtgt tggggatcga cccagggctt cgcaccggat gcaagtgcgc
     1741 ggtgatcgat tctaacggga tgtttttaga ccatgcgacg atttacccgc acgccccgca
     1801 ccacgcgaag tccgaaagtg aaaagatcct atcggagctc tatgagcggc accctttcga
     1861 gatcgtcgcg attggcaatg gaacggccag ccgagagacc gaggccttcg tcgccgattg
     1921 gatcgcaaaa aggaatcacg atgtctcata tctgatcgtt tcggaggcgg gggcgtcggt
     1981 ctactcggcc tctgaaaacg cggtcgaaga gttccccgag ctggacgtca cctcacgggg
     2041 cgccatctct atcgcgcgac gggtccagga ccctttggcc gaactcgtga aaataccgcc
     2101 cgaatcgatc ggcgtgggga tgtaccagca cgacctgccg gtgagtgagc tcaatcgcgt
     2161 attgaaaatg gaagtcgagt cggtcgtcaa ctacgtgggc gtcgatctca accaagcctc
     2221 tccctatctg ttgcaatacg tatccggact caaccgttcg aaggcctgga agatccacga
     2281 gtataaaacc gagttcggct tctttcggag ccgagaagac ctcaaaaacg tgaaagggat
     2341 aggggagaag acctacgagc tcgcggccgg tttttgccgg attccagaat ccgaaaaccc
     2401 tttggacaac acggtcatcc atcctgaatc gtacgagcgg atccatcgat tgttaaaaag
     2461 ggtcgggagc gccttcgaag aaatcggcct tcgcccggac gactttctca aaaaggtacg
     2521 atcgattggg catcaaaccc tatccacaga gctccaggtg accgagggag agctgaagga
     2581 ctatctggac gccctgacga tcagacacct cgatccgcgc gacagccttc cacagccgtt
     2641 gttgaaaaaa gaggtccgtg gtctggacga cctcggagag gggatggaac tggaagggac
     2701 cgtccggaac gtcgtggatt tcggagcttt cgtcgatata ggagtggaaa ttgacgggct
     2761 gatccaccag agccaatttg gtaagcgctg ggttaagccg tctgcgatcg tccacgtggg
     2821 agagatcatc cgagtgcgga tcttgaaagt ggataaagat cgaggaagaa taaatctcgc
     2881 ctttgttcag aaagcataga ttcactgtga tataatcctt tttacctttc atactatgag
     2941 gagtcgaggc cgtgcaggat gttcgttttt ttagcgccct atcgaaacaa tacgctgaag
     3001 gaaagctgaa taaggtcgag ctggaaacga ccctttttta cccggactcg gagggcgggc
     3061 agctcggtga ccgtggaaaa attggaaacg cggatgtgct gaaggtctac cgagtggacg
     3121 gcggtgcgtt ggttcacgaa gtttcggaac cggtgaccga taccggccga ccgatagagt
     3181 gcctgataaa tggctcgcga cgcttggata tctcgcagca gcataccgcc cagcatctgc
     3241 tttctgccga cattttcagc ctctacggat atgaaaccgt cggtttcgcg atgggcgagg
     3301 cttactcgtc catcgatatc gatggcgacc aatgggactg ggagaggtca gaaagcgccg
     3361 aaagaatgat gctggaacag atcatggcca aagtcgacgt cgagatcttg gaggttccaa
     3421 agaacgacat acacctctat ccgttgcgaa aaaagatgag cgagaagatc gagcagttgg
     3481 gtacataccg aatcgtcaag atcggtcaca tcgactattc tctgtgcggc gggttccacg
     3541 tctcaaatct ggcccaatta ggcacggtca aaatcatcaa atacgaaaag gtcaaatccg
     3601 attggacccg attgtactac ctcgcggggt tcagagccct aatcgactat cagaaaaaaa
     3661 ctcgcctcct ccacgaaacg acgcgctcgc tctccacctc ggtcgacgaa ctgaactatc
     3721 ggatacataa tattttaacc gaacatcaag aggcgaaaag aatcaaccga aagctctcgg
     3781 aaaacctggc gtccgcgctt tacaactctt tccgctcgca aaaggccaca gataacgtga
     3841 aaacggtcgt cgagatattg ccttcggaag agatcgcgaa agggctgagc ggcaagttac
     3901 tgcacgaaca gaacaccatc tccttccttc tctacgagcg gccgaaaggc aacggctaca
     3961 tcctctcttc gacgcaagaa aactggaacg cccagtcgat ctttcggaag gtcaaagagt
     4021 tcttggagat caaaggcggc gggaccgaaa agatgattca gggtacggtg gaaggcaata
     4081 tcaccgcggt caaagagaaa tgcgagtcca tcataaagag ttatttttcc tcgggaggat
     4141 tgttatgaag aaatacgtga tactgggtgt catcctgctc gttcttccgc tttctacgtt
     4201 tgctctgcag gttgggattg ctttcagcgg ggatttgcag gacgcgactc cctgcaccta
     4261 tctcggcgcg accgttcgca gttcctgggg cctggccggg ctcgagcttt ccgtgatgcc
     4321 gcagatttta cccgaactcg acttcaccag ctaccggttt atcatctctc cgtccatcgg
     4381 atggggaaca cctgagctca ggatattcgc cgggatcgcc ccccatctga tgttgaagaa
     4441 ggggcagttc gacttcgaca tcgcaaaatg gaatattggc gcaggcgctt cctttatgtt
     4501 cgcgaaaaac gtcatctgtt tcttcgattt tttcgtcggt ttcgacttca gaaactatga
     4561 gcaaatcgat aaactgacca ccggctcctt catcaccgtc ggtgtaacct ataacttcaa
     4621 cgtcgggttt tagaagggag gctctgattt ggccgaaaag tacaatctac aggaccccta
     4681 taccaacctg gcccgtagaa aaaaagagaa gatacgtatt ttcctggtca acgggtttca
     4741 ggtgcaagga aagttgaaat ccttcgacaa ctttacctat ttggtggagg atgggaagac
     4801 ccaaagcctg atctttaagc acgcgacgag caccattctt cctccgtctc cgatcaagtt
     4861 gccgcctccg aaaagcgaga aatgatataa tggccttagt tcttgtagtc atcgcggcgt
     4921 aagcgcttct tcttctcgcg acagcacgaa aagaaggggg ttcgcacaga gggcacagag
     4981 agcacggagg agagattaga accaattcag aattgaatat ggaacgtaag cctgggaacg
     5041 gcgcacttta gcgcggcatg gtttctggga acgccaactt ttaagttggc atgtcttaaa
     5101 agcgaaagac aggagagagc gaatcgcttg atttcattag tatcgcgggg tcgcaaaaac
     5161 cacttacccc gcgataaccc cacacgggca tcgcggcgta agcgcttctt cttctcgcga
     5221 cggcgcgata cgcatttgat tcttcatctc gttctgatac cttacaggcg tatagagaga
     5281 cagaggggat cgcgcagtcc tgttagttac tgcgcgatta cccacagaaa gcggatcgcg
     5341 gcgtaaaaag gcaaaaggcg gacctcctcc tcttatcttt tttgtgcgcc tcgatattat
     5401 acccacacta tttggaaaaa tgcgtctctt atgagataat tcggcatcat agttttaagg
     5461 aaaagggcgg ccttgtcgct tgctacccat ttttggtgca agactgacga agcctgcctt
     5521 tatgaaaaga cctcatccag ggggtttcgt tgcgagcagt cgggatcatt gtcggttttt
     5581 tatttgtttt aacagcgttt ggcagttttc agaaaataga aacggaattc ttcaatatcc
     5641 tatacgaata cgggcttgag gaacaagcgg agatcctgtt tcgagaaggc ccggaaattt
     5701 atcgtcggct tacggatttc tatcggatct accccgcgaa aaagttgaac gtttacctct
     5761 tagataacaa cgactacggt aacgcctacg ccgatttctt ttctcatgcg atcgtaatat
     5821 acgtcaatcg cgcaggcgcg tcgtatatca ataacaccta ccctttgtgg gttcccttcg
     5881 tcttctctca cgagctcacc catatcctta tcgcaaacaa acccttctgg ttcaagaact
     5941 ttttgaatat attcggccga cccgcagcga tgttttttga caccgccttt tcatcctcct
     6001 acctccacga ggggttgtcc atctattccg agtcgatgtt atcgggagaa gggcggttct
     6061 tcgacaccca gtttgatatg tacctacggc gagacctgta cgaatccagg ttctacgggt
     6121 tcagtttggc tgggggccct tcctctccca tttttaaccc ggtcgggatg aattacctgt
     6181 acggcgccag tttttttgag ttccttggag agcgatacgg ggcgaaggtc gtttcatccc
     6241 ttttgaacac gatgggagac cagtggatgt ctctgaagag ggccttggaa accgccacga
     6301 tgagacccta ctccgcttta ctggcagaat gggagagctg gtgtcgggaa aaagcctatg
     6361 ccctcctgcc caaaagcccc atagacgcgc ccgaaaatct tacggggacg ggttttttta
     6421 ccggccaccc catcgcaatc ggctcatatt tatactatct atcccattcc gaaaggggtt
     6481 ccgaaatcgt aagaagaaag ggcgacggtt ccgaagagcg gtgggaggtc ccgtatccca
     6541 gcgactatga tgtgagcccc aatgggcggt tagcgttcgt gtacgcgata ggagacggtt
     6601 tggagctcta cacacgctac ctctatttcg gagattgggg ggtggaattg tggaaaagcc
     6661 cattgataga gcgggtaatt gccctccggt ggatggacga ccgttggttg tgggtgaccg
     6721 cggttgaaaa cggtgggacg gccttgtggc tggtcgacac cgaaactggc aaaaagcaga
     6781 ggatgatatc cggcagcccg catttttacc tgaacagcct ctgtgtgctc tcggatatca
     6841 ccgagagccc gaaggcgatc gtgtgctcga tttcctacga cgggcaggcg cggctgtacc
     6901 ggatgccttt gatgatagac gagcgcgccg aaacttacga gtttgggaat tgggaggttg
     6961 tcggctctcc aaaaggtaac tcattggatc cggcctacgc ggacggggcc ctgtactacg
     7021 cgaacgacct ctccggcatc ttcaacctct accgatggga ggaggaaagc ggcctgatcc
     7081 aacagctcac caacgtcgct gtgggcgctt tccaaccggc gctcttcgat ggcgagctct
     7141 attacagcga atactccagg aacggctttg acctcttccg ggctccgaac tccgaaaccc
     7201 ctgctaccct gtcgctcgag ggatcgatga ccttttctcc cataccgacg cgctatgaag
     7261 cccttcgtgt ggaaatgagt ccggcccagg cgatccagcc tattcctcaa ccgcaaacgg
     7321 tcgttctatg gccgaagccg agaatatggc tcccgatcgt cacgctgacg ccgggaggtt
     7381 ttgggctctt cggcgggatg gtcggctggg acgacctcaa acgatgggtc tggtctttta
     7441 atggctatta tctgtcggag tcggagttga tgaacaccct ggacctgtcc ccgtggagca
     7501 tcggtgtcaa tctcgtttac cgcggcctga ttcctttcag cgcgagcctc ttgctgaacg
     7561 aagaggggtt cgtcgggcgg gttcagttag acaaatcctt cgtcgaccgc gtcaaccgag
     7621 acctcatacg gatgcagccg gcgatcggca tccagtggac gctaagcgac cgttctctat
     7681 gggcgttgca atgggaatat tatcaggctg tgggg
//
LOCUS       DAOPXN010000101         7700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9494_length_7700_cov_6.459516, whole genome shotgun sequence.
ACCESSION   DAOPXN010000101 DAOPXN010000000
VERSION     DAOPXN010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7700
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9494_length_7700_cov_6.459516"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1953)
                     /locus_tag="PKM49_06595"
     CDS             complement(<1..1953)
                     /locus_tag="PKM49_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82517.1"
                     /translation="MTRELIGLDFGTTNTHLSLTPVGEKNPVGRDIRVFPQNAVQTVL
                     LYAPKESRVIAFGQTAQEEWVSMSRSERRGLKLAANFKPITGADPVATKESDLFLTAL
                     FQHLQDERFLSHLSSDNHTQVVIGLPAEADPSYRGALSTIFTTMGYPHTPFFYEPWGA
                     LFYHYSTKQIDKDHLFRGVLVVDFGGGTLDCSYLKDFRVRQVWGSNVLGGQLLDDCLY
                     QLFLKQNPGVDKAFRDEAVEQYIRYILFREIKEKYSNRLTQNLTLPFRETIWVGKDHY
                     GDFEISDYSVFLSLLREYTVSEDLATSLSTIPHAGETLTREPFDLSAALKETVLKAFR
                     AQKVPKDAVSLILLTGGSSRWKFFQEMIAEEFPHTRILSSEDPEASISRGLGSGYSLV
                     LFEKQVKAEIHASMDEITDRLTSTYEEILHLSMDRSLQVTSDLLRAHAEPKITRFLEQ
                     GGTFTDLEKDLSAGLLELGESLTLAQEEIRARAFKQIETATQKEFYRWFDTRGPFFSR
                     QNDLLLPDPLMAQMIEKSLANRAFSTLTTVVSVSFGTILGGVIGLSGVAGAALSGPVG
                     WIGAFTVGVFLTLASFLGFKGKITERMKGVRYPPWMLKVLFPSKKKALERVFSAQTKE
                     LDRRAREEWLQFALEKPKLKQQIREYT"
     gene            2204..2821
                     /locus_tag="PKM49_06600"
     CDS             2204..2821
                     /locus_tag="PKM49_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82518.1"
                     /translation="MDYYSPEIIILSLSVYGLLLFPIDLALWRADNERTGRTSGARLW
                     LYYVALHLFISLSGILLLFFSGSLCFHTPLPSPMGPFLIAIAGLQLLFFSFAVFINQL
                     SKKLSNILVWAFSFFVFNVIFSFGTLAFAKIAIFQLVLRHFALDLSEWLPMTFAIRSA
                     RYFYSSNNAGHLGSSVLKAALYTFGFASASWLIAWTRRKRRTPSH"
     gene            complement(2842..3672)
                     /locus_tag="PKM49_06605"
     CDS             complement(2842..3672)
                     /locus_tag="PKM49_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82519.1"
                     /translation="MMLRINLFSKILFLILLSLYFVVVATPFLLMFFNGFKTTRELFL
                     KPFEMPDSFAPENHIRAWERAGLGTAFLNSIVVSGAGIFGVLFTSSMLAYVLSRYRFR
                     FRRFIYVYIILGLALPARLAIIPIFLLLNSLKLTDTRLGLVLVYIATGASFATFLLKN
                     FMDGIPLEIEESARMDGASPWTVYRRIALPLIKPALVVVGLVNFVNIWNDFFFPFIII
                     TSKAKETVPLAVSLFYGEYSNQWPMIAAALTISVVPIMVVFFLFSKQFISGITQGAIK
                     "
     gene            complement(3669..4571)
                     /locus_tag="PKM49_06610"
     CDS             complement(3669..4571)
                     /locus_tag="PKM49_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82520.1"
                     /translation="MSCKIRYILLFTIPALSLYTLFVIYPFGYSLFLSFFKWSGAGPK
                     LFVGLDNFKSILFGRMSQEFINALLHNFYFFALNALCELGVAFLIAIVLASRIWGQRF
                     FRTIVYLPNVISMVLVGFIWVMMLNPQWGLINQVLKAVGLKHLAQPWLGQPKTALTTV
                     ILVNVWRNIGFYILVFLAAIIGIPNDIFDAAYIDGAKNWRVIWKIIFPMTFSTFQTLL
                     ILIFIWSFNIFDVVYSLAGVQAGPVRSTDVLGTVFYRTAFGGLGSSGQDYGLGAAIVV
                     LIFLMVIPLSMIYALMMDRRKRVE"
     gene            complement(4640..5896)
                     /locus_tag="PKM49_06615"
     CDS             complement(4640..5896)
                     /locus_tag="PKM49_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY82521.1"
                     /translation="MKQLVFVVLCFALFFSLVSAKTVEIWSWRTQDAEVWQVVQKNLR
                     AKGEDITIDFRAFIPTEYDAKLFLALQGGTGPDIAYIRRLPGGRTQALVEGKLIKPLD
                     QAIDFSNFSESVQKNVTMDARLWGVPFAVQVCGIFYNEDIFKENKLSEPRTWDEMLEI
                     CKVLKAKGIDPFFCTAKEDWTLTMQHAMCGVSILGPAWIKRLTEGKVDFLDQDWIYLQ
                     TLLNDLKVYYQDGYMGNDTNDMNAAFAFGQAAMVFYGAWGYQTWKELNPDINVGYFMV
                     PPDWEGDNAYAYIYMDGAFSLTSIAKNPTDAMKVLKYTATPEFGTIFTGITANIPAVN
                     GAIMPDIPLLKEVMDAGANWASPYVYWVGSPFVVQQPSLYDGVLSPGMQAMYMGQITP
                     TELAQRAQDALSQWYKPLMRKLGKIK"
     gene            complement(5921..7063)
                     /locus_tag="PKM49_06620"
     CDS             complement(5921..7063)
                     /locus_tag="PKM49_06620"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNY82522.1"
                     /translation="MRVHLGLDRIEQFSSLFKNRAIGLVTNYSGVNGRLEENVGLFLK
                     CGFSVRKLFAPEHGIYGIADGEGFSDFIHPYYQLPVVSLYGEKTKPTPEDLEGLDLLV
                     YDIQDVGLRYYTFIYTLAYCLESAAERGIPVVVLDRPNPLGGLLISGNRIPEAHSSFV
                     GGLRLPIRYGLTPGELARYCLREKDLQVDLSVVPMTNYTRCTYFPDTGLLWNVPSIAL
                     PTFESVVCYNGGCFVEGFNLSEGRGSPKPFQMYGAPWIEMNRLYRYMKESDTEGFAFR
                     TRAFIPITSKYRNEVCFGLEFFPERKDADFIPVLLRFLKGVAETCPEQLQLVRYADVE
                     RLESLTGDPRVRDYLWGETPLDMLLNTWEIESSAFEEETSDIRMYP"
BASE COUNT         1867 a         2050 c         2016 g         1767 t
ORIGIN      
        1 ggtgtattcg cggatttgtt gcttgagttt cggcttttcc aacgcgaatt gcaaccattc
       61 ctctcgcgcg cgtcgatcca gctctttggt ttgggccgag aacactcgtt ccaaagcctt
      121 cttcttcgaa gggaagagga ccttcagcat ccacggggga tagcgtacgc ctttcatcct
      181 ttcggtgatc ttgcctttga agcccaagaa agacgcgagc gtcaaaaaaa ctccgacggt
      241 gaaggcgcca atccaaccca ccggcccgga caaggccgcc cccgccacgc cggaaaggcc
      301 tatcaccccg ccgagaatcg ttccaaaaga gacggagacg accgttgtga gggtagagaa
      361 ggcgcgattc gccaagctct tttcgatcat ttgcgccatc aggggatcgg gtaacaagag
      421 gtcgttttga cgggaaaaga aggggcctcg ggtgtcaaac caacgataga actccttttg
      481 cgtggccgtt tctatctgct tgaaggcacg ggcccgaatc tcttcctgcg cgagggttag
      541 agattctcca agttccaata gccccgcgct caggtccttt tccaagtctg taaaagtccc
      601 cccttgctcc aaaaaccggg tgattttcgg ctcggcgtgg gcgcgcaaga ggtcgctcgt
      661 gacctgaaga gagcggtcca tcgataggtg gaggatctct tcataggtag aggtcaaccg
      721 atcggtgatc tcatccattg acgcgtggat ctcagccttc acttgctttt cgaagaggac
      781 cagcgagtac cccgacccca accctcggct gatcgaggct tcggggtcct cggaagacaa
      841 aatcctggta tgtggaaact cttcggcgat catctcctga aaaaacttcc aacgactgct
      901 acctccggtc aggaggatga gtgaaaccgc gtcctttggg accttctgag ctcggaacgc
      961 cttgagtacc gtttccttta aagctgccga taggtcaaaa ggctcgcgcg tgagggtttc
     1021 accggcgtgt gggatcgtag agagggacgt cgccaggtct tccgacacgg tatattcacg
     1081 caatagactt aaaaaaaccg agtaatcgga gatttcgaag tccccgtaat ggtcctttcc
     1141 gacccatatc gtctcgcgaa agggaagggt tagattttgc gtgagccgat tcgaatactt
     1201 ctccttgatt tctcggaaca ggatgtatcg gatatactgc tcgacggcct cgtcgcggaa
     1261 cgccttatcg accccgggat tctgcttcaa gaataactga tagaggcagt catcgagcag
     1321 ctggcccccg agtacgttcg agccccagac ctgacgaacg cggaagtcct tcaaataact
     1381 gcaatcgagc gtgcctccac caaaatcgac gaccagaacc ccacgaaaga ggtggtcttt
     1441 atcgatttgc ttcgtggaat agtgatagaa gagggcgccc cagggttcat aaaaaaatgg
     1501 ggtgtgtgga tagcccatcg tcgtgaagat cgtggacagg gccccgcgat atgaaggatc
     1561 ggcttccgcc ggtaacccga tcaccacttg cgtgtggttg tccgacgata aatgggacaa
     1621 gaagcgctca tcttgcagat gctgaaacag cgcggtcaag aacaggtcgc tctctttcgt
     1681 cgccaccgga tcggcgcccg taatcggttt gaaattcgcg gctaacttca gtcctcgacg
     1741 ctccgagcgc gacatagaga cccactcttc ctgcgcggtt tgcccgaaag cgatgacccg
     1801 tgactctttg ggcgcgtaca gtaacacagt ctgcaccgcg ttttgtggaa aaacccgtat
     1861 atcccgaccc acggggtttt tctccccgac gggcgtcagg gaaagatgcg tattggttgt
     1921 accaaaatcc agaccgatga gctcgcgcgt caactatccg ctcctttctt ccgtcttatg
     1981 gattatagca aaaaatcgac attctgggtg tttttattcg gctctttagg ggggaaaggg
     2041 tattcacggg gttcattcgg agtttttgac gcggtttttt ggcaggtgtc ttcgttctcc
     2101 atcctacgct cttttttcgc ttttctatcc ctcggcgttc ctcatctgcg ttgggttgct
     2161 catacaaacc cctccgagcg tcctcttcgg aggaaagcgg tttttggatt attattctcc
     2221 tgaaatcatc attctgtccc tgtccgttta cgggctgctg ctttttccga tagacctcgc
     2281 gctttggagg gccgataacg aacggacggg acggacgagc ggcgcgcgtt tatggctgta
     2341 ttacgtggcg ctgcatcttt tcatcagtct aagcgggatt ttgcttctct ttttttccgg
     2401 atcgctctgc tttcataccc cgttaccgtc acctatgggt ccgtttctaa tcgccatagc
     2461 cgggctgcag ctcctctttt tttccttcgc cgtgttcatc aaccaactct cgaagaagct
     2521 ttcaaacatc ctcgtctggg cgttttcttt cttcgttttc aacgtgatct tcagtttcgg
     2581 aaccctggcc ttcgcgaaga tcgcgatctt ccagctcgtc ttgcgacact tcgcgctcga
     2641 tctatccgaa tggcttccga tgactttcgc aatccgtagc gcccgatatt tttacagcag
     2701 caataatgca ggtcatcttg gtagcagcgt cctgaaagct gcgctgtaca cctttggctt
     2761 tgcgtcagcg tcttggctga tcgcttggac gcgaagaaag cgccgaacgc cctcgcattg
     2821 accggaatcg tagtggcggg gctatttgat ggcgccttgc gtgattcccg atatgaattg
     2881 cttcgagaaa aggaaaaaga cgaccataat cggcacgaca gagatggtta gcgctgcggc
     2941 gatcatcggc cactggttcg aatactcccc gtagaacaac gacacggcga gtgggaccgt
     3001 ctcttttgcc ttcgaagtga tgatgataaa cgggaagaaa aaatcattcc agatattgac
     3061 gaagttgacg aggcccacga cgacgagggc gggctttatc aacggcagcg cgatccttct
     3121 gtagacggtc cacgggctcg cgccgtccat tcgggcggac tcttcgatct ctagcggaat
     3181 gccatccata aagtttttca gcaagaaggt agcgaaagag gcgccggtgg cgatgtacac
     3241 caggaccaaa cccagtctcg tgtccgtcag cttcaggctg ttcaagagca gaaagatcgg
     3301 gataatcgcg agtctcgcgg ggagggccaa ccccaagatg atgtagacat agatgaaccg
     3361 gcgaaaccgg aagcgatacc gcgagagaac gtaggccaac atcgacgagg tgaagagcac
     3421 accgaaaatc cccgcgccag acacgacgat gctgttcaaa aaagctgtgc ccagaccggc
     3481 tctttcccac gcccggatat gattctccgg cgcgaagctg tcaggcatct caaatggctt
     3541 caaaaacagc tcccgcgtgg ttttaaaccc attaaaaaac atcagtaaaa acggggtggc
     3601 cacgaccacg aagtacaggg acagtagaat taaaaagagg attttcgaaa agagattgat
     3661 ccgcagcatc attctaccct ctttcttcga tccatcatga gcgcatagat catagacaac
     3721 gggatcacca tcaaaaagat caacacgact atcgcggcgc ctaagccgta atcttggccg
     3781 gaactaccca aaccgccgaa ggcggtgcga tagaacacgg tccccaacac atcggtcgaa
     3841 cgaaccgggc ccgcttggac ccccgcgagg gagtaaacca cgtcgaagat gttgaaagac
     3901 cagatgaaga tcagaatcag aagggtctgg aaggtcgaaa aggtcatcgg gaagatgatc
     3961 ttccagatca cgcgccaatt tttcgcgcca tcgatatagg cggcgtcgaa gatgtcgttc
     4021 gggatcccga tgatcgcagc aaggaagacc aggatataaa aaccgatatt tctccacacg
     4081 ttgacgagaa tgaccgtggt gagcgcggtt ttcggctgac ctagccaggg ttgggcgagg
     4141 tgtttcaatc cgacggcttt caacacttga ttgatcaatc cccattgcgg attgagcatc
     4201 atcacccaga tgaacccgac gaggaccatc gatatcacgt ttgggagata gacgatcgtt
     4261 cggaagaatc gttgccccca aatgcgggac gccaggacga tcgcgatgag aaaagcgacc
     4321 ccgagttcac atagcgcgtt gagcgcgaag aaatagaagt tgtggagcag cgcgttgatg
     4381 aactcctgtg acatccggcc aaagaggatg cttttgaagt tgtccaaccc gacaaacagt
     4441 ttggggcccg cgccggacca tttaaaaaag cttaaaaaca agctgtaccc aaaggggtaa
     4501 atgacaaaga gggtatacag agacagcgcc ggtatcgtga acaacaggat gtatctgatt
     4561 ttgcaagaca aggaaacgcc ccctttttag aaaacgagtg cagggagaga actccctgca
     4621 caccgtagcg aaacgaaggt tatttaatct ttcccaactt cctcatcaga ggcttatacc
     4681 actgtgataa agcgtcttgc gcccgttgcg cgagctcggt cggcgtgatc tgtcccatat
     4741 acatcgcctg cataccggga gataagacgc cgtcgtaaag ggatggttgt tgtaccacga
     4801 aaggtgagcc gacccaatag acgtagggcg aggcccagtt ggctccggcg tccataacct
     4861 ctttcaacag cggaatatca ggcattatgg ccccgtttac ggcgggaatg ttcgcggtaa
     4921 ttccggtgaa gatcgttccg aactcaggcg ttgcggtgta tttcaggact ttcatcgcgt
     4981 cggtcgggtt tttcgcgata gaggtcagac tgaaggcgcc gtccatataa atgtacgcgt
     5041 aggcattatc gccttcccaa tccggtggca ccataaaata cccgacgttg atatccggat
     5101 tcagctcttt ccaagtctgg taaccccaag cgccgtagaa aaccatcgcg gcttgaccga
     5161 aggcgaaggc ggcgttcata tcgttggtgt cgttccccat ataaccgtct tggtagtaaa
     5221 cctttaaatc gttcagcaac gtctggaggt aaatccaatc ttggtctaga aagtccactt
     5281 ttccttcggt gagccgtttg atccaagcag gcccgaggat actgaccccg cacattgcgt
     5341 gctgcatggt caaagtccaa tcttctttcg cggtacagaa gaagggatcg atccctttag
     5401 ctttgaggac tttgcagatt tcgagcattt catcccacgt tctgggttcg ctcaacttgt
     5461 tctctttgaa gatgtcttcg ttgtagaaaa tcccgcaaac ctgtacggcg aacggtacgc
     5521 cccaaagtcg cgcgtccatc gtcacgttct tttgaacgct ctcagaaaaa ttggagaagt
     5581 cgatggcttg atccaagggt ttgatcaatt tcccctcgac gagcgcttgc gtccttccgc
     5641 ccggaagtct tcggatatag gcgatgtcag gtccagtacc gccctgcaag gccaagaaca
     5701 gtttcgcgtc gtattccgtc ggaatgaacg cgcgaaaatc gatggttatg tcctctcctt
     5761 tggccctcag gttcttctga accacttgcc aaacctcggc atcttgcgtg cgccagctcc
     5821 aaatttctac ggttttggct gaaacgagcg aaaaaaacaa ggcgaagcat aagactacga
     5881 aaaccagctg tttcacagaa atccctccgt taaagtggtt ttatggatac atccgaatat
     5941 cggatgtctc ttcttcaaaa gccgaggatt cgatctccca ggtgtttaag agcatatcga
     6001 gcggggtttc cccccacaga tagtctcgca cacgcggatc tccggtcaag gattctagac
     6061 gctcaacgtc cgcgtatcgt acgagctgca actgttccgg gcaggtctcg gccaccccct
     6121 ttaggaaacg caatagaacc gggatgaagt ccgcgtcttt ccgttctggg aaaaactcca
     6181 aaccgaagca aacctcgttt cggtatttgg aggtgatcgg gatgaaggcc cgggtccgga
     6241 acgcgaatcc ctccgtgtct gactctttca tataccggta taaccggttc atttcgatcc
     6301 aaggcgcgcc gtacatctgg aagggcttgg ggctaccgcg gccttccgag agattgaacc
     6361 cttccacaaa gcaaccgccg ttgtaacaca ccaccgattc gaaggtgggc agcgcgatgg
     6421 agggcacgtt ccacagtaag ccggtatccg ggaaataggt gcacctagtg tagttcgtca
     6481 tcgggacgac cgacaggtct acttggaggt ctttttccct cagacagtac cgagccaatt
     6541 ctcccggggt caagccatac cgaatcggga gccgaagacc gccgacgaaa gaggaatgcg
     6601 cctcgggtat gcgatttccg gagatcagca gccctcccaa gggattcggg cgatcgagca
     6661 cgacaacggg aataccccgt tccgcggccg attccaaaca gtacgccaaa gtatagatga
     6721 aagtatagta cctcagtccg acgtcttgta tgtcataaac gaggagatcc aacccttcga
     6781 ggtcttccgg tgtcggttta gttttctctc catagaggct gacgacgggt aactgataat
     6841 aagggtgaat gaaatccgaa aacccctccc catcggcgat accgtaaatc ccatgctcgg
     6901 gggcaaagag cttccgaacg gaaaacccgc acttcagaaa gagccctacg ttttcctcca
     6961 agcgcccgtt gacgcccgaa tagttcgtga cgagcccaat agcgcggttt ttgaagagcg
     7021 aagagaattg ttcgattctg tccaatccta agtgaactcg cacctggaac cctccttttt
     7081 caaggactgc ttgagtgtac acgttcctat cttgaaagta aagtcatcgc atttcatgaa
     7141 gtcctgagct cacgccttga catcgatttc tataagaccg acaaaagaaa gtatccgggg
     7201 ctcctcaagg cggcgctcgt tcactagggg cccgactgtc gatgtactat tctacacgat
     7261 agaaagcgaa aggtcaatcg tgagtatgag gtaagttatg aaggaccatc aagaaacaaa
     7321 gccaaaatcg atcgcgcgat ttttagtcaa tgaagtcgtc gcggcgtaag cgccccttct
     7381 tttcgcgacg gcgcggtacg cttttgtgcg agggcatcgc ggcgtaagcg ctccttcttc
     7441 tcgcgacggc gcgatacccc ggttttgttc gttccaaaga tcaaaaaaac agggttcgca
     7501 cagaggacgc agagaacacg gagagagcag gcatcgcggc gtaagcgctt cttcttttcg
     7561 cgacggcgcg ataccccgtt tttcgttcgt tccaaagatc aaaaaagggt tcgcacagag
     7621 ggtacagagg aaaaaatggc aaagcgtggg gatcgcgggg tcgtaaaaac cacttacccc
     7681 gcgataacct tgtaggcatc
//
LOCUS       DAOPXN010000102         7699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9496_length_7699_cov_5.117870, whole genome shotgun sequence.
ACCESSION   DAOPXN010000102 DAOPXN010000000
VERSION     DAOPXN010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9496_length_7699_cov_5.117870"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKM49_06625"
     CDS             complement(<1..248)
                     /locus_tag="PKM49_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82523.1"
                     /translation="MPGYRTHSLSAFLTFPLVTLLYKCLAPLTKGTPLSWDQISTGFV
                     LYLLSSQAPDIDHKQAYSNRLFFFFLWMVLTLAVFRAL"
     gene            complement(251..2092)
                     /locus_tag="PKM49_06630"
     CDS             complement(251..2092)
                     /locus_tag="PKM49_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82524.1"
                     /translation="MKGRLFLCFLALSALCFAVRPLSIEEARALVPQIEVDTFSITCY
                     GTRYAVEKEWQGAPRIHFLWDSQTGYENLVEHVFEEAGYLKTPAGETFRGKILSIGED
                     FIYWEFSFRGKLVTNRDAVVRKDERLPDSIWRFCALHYGLSKPDETGENKSFSYLVLL
                     KNRQPYEPADPQANQTSVLLLEGQGFSIWKFSDTHVRLKTLSTQKASERPVWQFAIAP
                     VPVQAIDLEDAFFPFTLETRPPGLLARLDGVEYETPFSAQLPQGVHRLEIAGEERFFY
                     LSEPTTQFLEIEQPQGTLSIELSLTARIALYRDRETVFITQSATRVTQTLPFGFYTLK
                     VQREAYEPIEETVEVLPGKRVTKSYSLTPIPGAVFLKRSLDQPCERIFVNERWAILAD
                     STRSVFMPLPTNDATAFYLEERVAGAEGPFSFSLRTVYLGEAPIFLNPFPIVTVVPFD
                     KELWIFDASGTLTVVNTRNNKTLWQRAVGYIPHWTYRYDRWLALLDIYGNFILLEPQR
                     GYHEVFRYRFGALKTLDHVVVTPSALLLYFSPDALLTYSFNTRAYHYEEGVSYEPERI
                     LLSTNGFQKGRDLFYRFEEPLRAYDISDGHIAVLYDRYYAVLFAPGE"
     gene            complement(2181..3701)
                     /locus_tag="PKM49_06635"
     CDS             complement(2181..3701)
                     /locus_tag="PKM49_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82525.1"
                     /translation="MKKVLVSVLLVVLLCVSAFSAIVDYVPADASFALVMRNNASQYE
                     QLKKVGIFGFLLRDMGLESMIAQQFESMKYTDPSFKPENFWGLLGGDIAAFSQGEFDF
                     SVFGDLFGFASDPDDLLPEMMDRLQDLSMVFIVKPTADPDLVIKTWNKALGMEMAWGV
                     DPYSGMMLEKSDGYILFGTNAQAIAAAKAAKTNNILNNPHFRKEYDAGHWMVLYSKSQ
                     METESVSDLLESNLGLKIPTDILEGVETDYSIATLRVGEALELRQVTYFIYSSDSLKR
                     FVIDANADLADLYKKTSIPGTIQGLMAVNKIEEVWKNVEPVFNDIINQLIEKGEMDET
                     DKSTAEKVFGFIKGFKGLMKFGMDITLDESLNPLVDLFLKIDMDKMADLQDWARKQSE
                     TAFVAKDGYLESVIMAKNKGDDFDFNLYLGQDEIILSTLKAKQLKSVDSLPVLAKNLM
                     FAELSNRYSFKKGNAMVFADIGSILTKILGVAYPSGLFLEFGADENANVRMIAVIK"
     gene            complement(3698..4681)
                     /locus_tag="PKM49_06640"
     CDS             complement(3698..4681)
                     /locus_tag="PKM49_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82526.1"
                     /translation="MDDAIGKAFVEINGLTKEFGEVKALREVSLSIRENELVGLLGPN
                     GAGKSTLSKILVTLLKPTFGEVRIAGYRLSEHPQKIREIMGYVPAQPTLYPHLTAWEN
                     LILFADFYGIKERTARAAIEELLEAFDLWDFRHKTLRKFSTGMIQKVNICRGLIHDPK
                     FLVLDEPFNGLDPVSRSVVKAYLTRMIQKGKTILLTTHFLDSVDDFINNVIILNHGKV
                     FSQAETQTLKEDFLRKQKTLRIEVKGTLIADSLREGEPIGGLSEPIRLKEIKGEDFFV
                     FEIESFDRLEEKLRIIQSLFSRVSYFRMKDPTLEEIFISLTKKTKPSEVQV"
     gene            complement(4681..5091)
                     /locus_tag="PKM49_06645"
     CDS             complement(4681..5091)
                     /locus_tag="PKM49_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82527.1"
                     /translation="MEISIDRLLNILVSQVESLSAAVEDLRLKQNVVGTVLMDAGLVN
                     EEKIKNAVKKQFHVMKSLNAEENYTEEEISLFTKEIVKWFQCDILSIRQDLERIQHML
                     KQMAKDAPKQEKGRIQIATPGLLNDLDRLKKTKM"
     gene            complement(5152..5982)
                     /locus_tag="PKM49_06650"
     CDS             complement(5152..5982)
                     /locus_tag="PKM49_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82528.1"
                     /translation="MEWKRLRRKFAKNPLNLISVVVFALFLAMAFFPQIFTQFEPNRM
                     NSAVRMISPTASHIFGTDQFGRDIYSRVVHGVRETLSIAFLSIVLACVLGTALGVISG
                     FYGRWIDQIVMRLMDAMFAFPTIILALFIIALYGASQKNLIIALGIVYTPIFTRTVRG
                     STISIKQFLYIKAAKALGKRDLSIMLKDVLPNLTSVLIVSFTTNFSTAVLSEASLSFL
                     GLGVPPPTPTLGGMVGQGSNFLLSAPWIALFPGLFIALIVLSINIIGDGLRDVLDPRY
                     "
     gene            complement(5997..6950)
                     /locus_tag="PKM49_06655"
     CDS             complement(5997..6950)
                     /locus_tag="PKM49_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82529.1"
                     /translation="MIATYIFGRVLSAVPTILILTFIVFLAIHLVPGDYIDIMLGTQN
                     YLTDEQLSGLYKQYGLDKPFVVQYGIWLKNLITFNFGTSLRTGQKVSAMILEKLPVTF
                     ELAFFALFFSLVFGIPLGITSAVKRNRFGDYVVRVLGLIGLSAPTFWIGAILIVAVSG
                     LFQDYTLFGFVSFQKDMAKNLQILLIPSLTLGLMLAAQIMRMARSSMLDVLSQEYVKV
                     AKAKGVSTWKVIYRHALKNAMIPVITTAGIQFGYLMGGTILIENMFALPGLGRLLLQS
                     VNQRDYPVIQGVVLFTGVFIVLMNILVDILYTLIDPRIDLR"
     gene            complement(6992..>7699)
                     /locus_tag="PKM49_06660"
     CDS             complement(6992..>7699)
                     /locus_tag="PKM49_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82530.1"
                     /translation="GINNAAKPFDNILVRRALSYAINREELVEMVAFGEGTVTGPLNP
                     SLKTWALLPEAFDEYSYNPEKAKALLKEAGYPNGFSFEAVTYAQYSLDKIGEAIQAQL
                     ALVGIQMKVDVAEFGIFVKRWREIDFQTFLSLNGGSTEPDIQFYRTFKSDGATNKFNF
                     KNAKVDELLEKGRQALDLEERVEIYRALQRLLVEENPFLFLYSPNNLYVSLPEVKGFV
                     PMSNQSLIMLKKTWLDK"
BASE COUNT         1885 a         1977 c         1983 g         1854 t
ORIGIN      
        1 aacagggcgc ggaaaactgc cagcgtcaat accatccata agaagaaaaa gaacaggcgg
       61 ttcgagtagg cttgtttatg atcgatgtcc ggtgcctgcg aagagagcag gtacaagacg
      121 aaccccgtcg aaatctgatc ccacgacagg ggcgtaccct tcgtcaacgg cgccaaacat
      181 ttgtagagga gcgtaacgag gggaaaggtc aagaaggccg aaaggctgtg cgttcgatac
      241 cccggcaccc ctattccccc ggcgcgaaaa ggacggcgta atatcgatcg tacaggacgg
      301 cgatgtgccc gtcggagatg tcgtaggccc gtaggggctc ttcgaaccga tagaacagat
      361 ctcgaccttt ttgaaaaccg ttggtagaga gaaggattcg ctcgggttcg taggataccc
      421 cttcttcgta atgatacgcc cgcgtgttga aagaataggt taatagcgca tcgggcgaaa
      481 agtagagcag aagcgcgctt ggagtaacga ccacgtggtc gagcgtcttc aaggcgccga
      541 agcgataacg aaaaacctcg tggtatcccc gttggggctc gagtaggatg aagtttccgt
      601 atatatccaa gagggccaac caccgatcgt agcggtatgt ccaatggggg atgtaaccga
      661 ctgcccgttg ccacagggtc ttgttattcc gtgtgttcac cacggtcagc gttcccgagg
      721 cgtcgaagat ccaaagctcc ttgtcgaagg gcacgacggt gacgatagga aaagggttta
      781 gaaaaatggg cgcctcgccc aaataaacgg tccggagcga aaaggaaaac ggcccctctg
      841 cgccggcgac tcgctcttcc aaataaaaag cagtggcgtc gttcgttggc agtggcataa
      901 acaccgagcg tgtggagtcc gcgagaatgg cccacctctc gttgacgaag attcgttcgc
      961 agggttgatc cagcgatcgc ttcaggaata cggcgcccgg aatcggggtc agcgaataag
     1021 atttagtcac acgcttccca ggcaggactt ccacggtttc ttcgatcggc tcgtaggctt
     1081 ctcgttgtac cttcagcgtg tagaaaccga agggcaaggt ttgggtgaca cgcgtcgcgc
     1141 tttgagtaat aaagacagtc tcccgatcgc ggtagagcgc gattcttgcg gttaacgaga
     1201 gttcgataga caacgtgccc tgaggctgct cgatttccag aaactgcgtg gtcggttcag
     1261 ataggtagaa aaagcgttcc tcaccggcga tctccaagcg atgcacaccc tgcggtagtt
     1321 gcgcggagaa cggggtttca tactctacgc cgtccagacg ggccaaaaga ccgggcggac
     1381 gggtttccag cgtgaaggga aagaaggcgt cttcgagatc gatcgcttgc acgggcacgg
     1441 gcgcaatcgc gaactgccac acaggccgct cgctcgcttt ctgagtcgat agggttttca
     1501 atcggacgtg cgtatcggaa aacttccata tagagaaccc ttggccttct agaagcaaga
     1561 cgcttgtctg gtttgcctgg ggatccgccg gttcgtaagg ctgccgattc ttcaggagga
     1621 cgagataaga gaatgacttg ttttcgccgg tctcatccgg tttcgacaat ccatagtgaa
     1681 gggcgcagaa acgccaaatg gaatccggaa gtctctcgtc tttacgaacg accgcgtctc
     1741 tgttggtaac caatttcccc cggaaagaga attcccagta gatgaagtct tcccctattg
     1801 ataggatctt accgcgaaac gtctctccgg caggtgtctt caggtagccc gcctcttcaa
     1861 aaacgtgttc aacgaggttt tcgtaccctg tctgtgaatc ccaaaggaaa tgaatccgag
     1921 gggccccttg ccattctttt tcgacggcgt accgggttcc gtagcacgtg atcgaaaagg
     1981 tgtcgacctc gatctgcggg accagagcgc gcgcctcttc tatagagaga gggcggacgg
     2041 cgaaacagag cgcggacagc gcgagaaagc acagaaataa tcgacccttc aagcgaacct
     2101 cctcgcaaaa aacccgggag aagcaacgcg cggttgcctc cacccgggtc aattcaaatt
     2161 tgatgtatga cgaattatgc ttatttaatg accgcgatca ttcgaacgtt agcgttttca
     2221 tcggcgccga attcgaggaa taggccggag ggataggcga cccctagtat tttcgtcaaa
     2281 atcgaaccga tatcggcaaa gaccatcgcg ttgcccttct tgaacgagta acgattggaa
     2341 agctccgcga acatcaggtt ctttgccaaa acaggcagac tgtcgacaga cttcaactgt
     2401 ttggctttta gcgtggagag gatgatttcg tcttggccaa ggtatagatt gaaatcgaag
     2461 tcatcgcctt tgtttttcgc cataatcacg ctttccaaat aaccgtcctt tgccacgaag
     2521 gcggtttccg attgtttgcg cgcccaatct tgcaggtcgg ccatcttgtc catatcgatc
     2581 ttgaggaaca agtcgaccaa cgggttcagg ctttcgtcta gggtaatatc cattccgaac
     2641 ttcattaacc ctttgaagcc cttgatgaaa ccgaacacct tttccgcagt ggacttgtcg
     2701 gtttcgtcca tttcgccttt ttcgatcagc tgattgatga tgtcgttgaa tacgggttct
     2761 acgtttttcc agacttcctc tatcttgttc acggccatca agccttggat agttcccgga
     2821 atcgaggttt ttttgtacag gtccgcgagg tccgcgttcg catcgatcac aaaccgcttc
     2881 aagctgtcgc tggaatagat gaagtaagtg acctggcgta actctagcgc ctctcccacg
     2941 cgcaacgtcg ctatggaata gtcggtttcc acgccttcga ggatgtccgt gggtatcttg
     3001 aggcctaaat tggactccag taggtcggaa acgctttcgg tctccatttg gctcttggaa
     3061 tacagaacca tccaatggcc cgcgtcatac tcttttcgga agtggggatt gttcaggata
     3121 ttgttagttt tggccgcttt ggcggcagcg atggcttgcg cgttcgttcc aaacaatatg
     3181 tatccgtccg atttttctag catcattccc gaatacgggt ccacgcccca ggccatctcc
     3241 attcccagcg ccttgttcca agtcttgatc acgagatcgg ggtcggcggt aggttttacg
     3301 atgaaaacca tagacaagtc ttgaagccgg tccatcatct ccggaaggag atcgtccggg
     3361 tccgaagcga atccaaacag gtcaccgaag acagaaaaat cgaattcccc ttgactaaac
     3421 gcggcgatgt cgccccccaa caacccccaa aagttctcgg gtttaaaact cgggtccgta
     3481 tacttcatac tttcgaattg ttgcgcgatc atactttcca atcccatatc tctgagtaga
     3541 aacccgaaaa ttccgacttt cttgagttgt tcgtattgcg aggcattgtt ccgcatcacc
     3601 agcgcgaaag acgcgtcagc gggcacgtaa tccacgattg cggaaaacgc gctaacgcac
     3661 agtaaaacaa ccaataggac cgacactaac acttttttca tacttgcacc tccgaaggtt
     3721 tggttttttt tgtcagcgaa atgaatatct cttccaacgt ggggtctttc atacggaaat
     3781 acgagaccct tgaaaagaga gattggatga tacggagctt ttcttctaga cgatcaaagg
     3841 attctatttc aaagacgaaa aaatcttctc ctttgatttc cttgaggcgg atcggttccg
     3901 agagcccgcc gatgggctcg ccttccctaa gcgagtccgc aatcaaggtc cccttgacct
     3961 ctatgcgcag cgtcttctgt ttccggagaa agtcttcttt caaggtctgg gtttctgctt
     4021 gcgaaaagac tttcccgtga ttgaggatga tcacattatt gataaagtca tccacagaat
     4081 ccaaaaaatg tgtggtgagc aagatcgttt tccctttttg gatcatacga gtcaaatagg
     4141 ctttgactac cgagcgtgaa acggggtcaa gtccgttgaa tggttcatcg agaaccagaa
     4201 acttcgggtc gtgaatgaga ccgcggcaga tattcacttt ttgaatcata cccgtagaga
     4261 acttccgtag cgtcttgtgc cggaaatccc ataggtcgaa ggcctcgagt agctcttcta
     4321 tcgcggcgcg agccgttcgt tctttgattc cgtaaaaatc agcgaacaaa atgaggttct
     4381 cccaagccgt caggtgcggg tacaaagtgg gttgggcggg cacgtagccc ataatctccc
     4441 gtatcttctg cgggtgctcg cttagtcgat acccagcgat gcggacctct ccaaaggtcg
     4501 gttttagaag cgttacaaga atcttggaga gagtggactt accggccccg ttggggccca
     4561 atagaccaac cagctcgttc tcacggatag ataaggatac ctcgcgtagc gccttgacct
     4621 caccgaattc cttcgtcaaa ccgttgattt cgacgaaggc cttgccgatc gcgtcatcca
     4681 tcacattttc gtttttttca accggtccaa atcgttcaac agtccgggcg tcgcgatctg
     4741 gatacgtcct ttttcctgtt tcggggcgtc ctttgccatt tgtttcaaca tatgctggat
     4801 gcgttccaag tcttgtctta tcgataaaat atcgcactga aaccatttga cgatttcttt
     4861 cgtgaaaagc gaaatctctt cttcggtgta gttttcttca gcgttaaggc ttttcatcac
     4921 gtggaactgt tttttcaccg cgttctttat cttttcctca ttgaccagtc cggcgtccat
     4981 caaaacggtg ccgacgacgt tttgtttcaa tcgtaaatcc tctactgcgg ccgagaggga
     5041 ctcgacttgg gagacgagta tgttcaacag cctatcgatc gagatttcca atggtttttt
     5101 cctccctttc tttccgattt attcgcccta ttgtatcaca aagagcccgc cttaataccg
     5161 cgggtcgagg acatccctca gaccatctcc gatgatgttt atgctcagta cgatcagcgc
     5221 gataaacaac cccgggaata gcgcgatcca cggggccgac aacagaaagt tgctcccctg
     5281 tccgaccatc cctcctaggg tcggcgtcgg cggtggtacg cccaggccta agaagctcag
     5341 cgaagcttcc gacaagaccg cggtcgaaaa gttcgtggtg aagctgacaa tcagcaccga
     5401 ggtgaggttc ggaagcacgt ctttcaacat aatgctcaaa tcgcgtttcc ccaaagcttt
     5461 cgcagctttg atgtataaaa attgtttgat cgaaatcgta gaacctcgaa cggttcgggt
     5521 gaagatcggg gtatagacga tacccaaggc gatgatcagg ttcttttggc tggctccgta
     5581 cagcgcaatg atgaatagcg ccaagattat ggtgggaaag gcgaacatcg cgtccattaa
     5641 ccgcatcacg atttggtcta tccaccgacc gtaaaaaccg gaaataaccc ctaaggccgt
     5701 tcccaaaaca caagccaata cgattgatag aaaggcgatg gagagtgtct cgcgtacccc
     5761 gtgaacgacg cgtgaataga tatctcgacc gaattggtcg gttccgaaga tatgtgaggc
     5821 cgtgggagag atcatccgaa cggcgctgtt catccgattg ggttcgaact gggtgaagat
     5881 ttgcggaaaa aaggccatcg ctaagaacag ggcgaagacc accacactga ttaggttcag
     5941 aggattcttc gcgaacttcc tcctcaatct cttccactcc atgttttctc cttaggctac
     6001 cttaaatcga tgcgtgggtc gataagcgta tacagaatgt caacgaggat attcatcagt
     6061 acgatgaaaa ccccggtgaa caacacgacg ccttggatca cggggtagtc gcgttggttg
     6121 accgattgga gcaacagtcg tcccaagccg ggcaacgcga acatattctc gatcagaatc
     6181 gtcccgccca tcaagtatcc gaactgtatg cccgcggtgg tgatcaccgg tatcatcgcg
     6241 ttttttaagg cgtgtcgata gatcaccttc caggtcgata ctcctttggc tttcgccacc
     6301 ttcacgtact cttgcgacag aacgtccaac atcgaactgc gcgccatccg cataatttgg
     6361 gccgctaaca tcaaccccag cgtcaacgag ggaatgagga gaatctgcag gttcttggcc
     6421 atatcctttt gaaatgagac aaacccgaac agggtgtagt cctgaaatag gccggaaacc
     6481 gcgacgatca gtatcgcgcc gatccaaaaa gtgggcgccg aaagaccgat caatcccaaa
     6541 acccgcacaa cgtagtcgcc gaaacggttg cgtttcaccg cggatgtgat ccctagggga
     6601 atcccgaaga ccaaactgaa gaaaagagcg aagaaggcga gctcaaaggt caccgggagc
     6661 ttttcaagta tcatcgcaga aaccttctgg cccgttcgca aagaggtccc gaagttgaag
     6721 gtgattaggt ttttcaacca tatcccgtat tgtacgacaa aaggtttatc taagccgtac
     6781 tgtttataca gtccggataa ttgctcgtcg gtcaggtagt tttgggtccc gagcataata
     6841 tcgatgtagt cgcctggaac gagatggatg gcgagaaaca cgatgaaggt caagatcagt
     6901 atcgtgggga cagcggagag aactcgtccg aaaatgtaag tcgcgatcac ggtgagatta
     6961 gaaccccccg ccgaagcggg gggtttccat tctatttatc caaccacgtt tttttgagca
     7021 taatcaggct ctggttgctc atcggcacga agcccttaac ctccggtaaa gacacataga
     7081 ggttgtttgg agagtaaagg aagaggaacg gattctcttc gaccagaagc ctctgaaggg
     7141 cgcgataaat ctcgacgcgt tcttctaagt ccagcgcctg acgccccttc tcaagaagtt
     7201 cgtcgacttt cgcgttcttg aaattgaact tgttggtcgc gccatcggat ttgaaggtcc
     7261 gatagaactg gatatcgggc tccgtggacc cgccgttgag agaaaggaag gtttggaagt
     7321 cgatctcgcg ccatcgcttg acgaaaatcc cgaactccgc gacgtcgact ttcatctgga
     7381 tacccacgag ggccagctgc gcctgaatcg cctctccgat tttgtcgaga ctgtattggg
     7441 cgtatgtaac ggcctcgaaa gagaaaccgt tcggatagcc ggcctctttt aacaacgctt
     7501 tcgccttttc ggggttgtaa gagtattcgt caaaggcttc gggcaacaac gcccatgtct
     7561 tcagagacgg gttcaacgga ccggtaaccg tgccttcgcc gaaagccacc atctccacga
     7621 gttcttctcg gttgatcgcg tagctcaaag ctctccgaac caaaatgttg tcgaagggtt
     7681 tcgcagcgtt gttgatccc
//
LOCUS       DAOPXN010000103         7683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9518_length_7683_cov_7.172391, whole genome shotgun sequence.
ACCESSION   DAOPXN010000103 DAOPXN010000000
VERSION     DAOPXN010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9518_length_7683_cov_7.172391"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1475..1918
                     /locus_tag="PKM49_06665"
     CDS             1475..1918
                     /locus_tag="PKM49_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014557922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGGC domain-containing protein"
                     /protein_id="HNY82531.1"
                     /translation="MEKVKVGIIICSRYRNCGGGKCLRAARNLEGGFSVHEGKEVEIV
                     GYSSCGGCPGGNVEYVPAEMKKNGAQYIHLATGMIVGYPPCPNLRYFKRFIEEKYGLE
                     VILGTHPIPENYFRTHLNLRTWDSPTWQEIIDPLSRDEALRKAYD"
     gene            2549..2842
                     /locus_tag="PKM49_06670"
     CDS             2549..2842
                     /locus_tag="PKM49_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82532.1"
                     /translation="MSRQKFTIEEIVEILSAEKKSSVAGVCRKHGISASTFYRWRERY
                     AAARDQSGAYSLDIAKKLVALEAENAALKKALAEKELEYTVLREFIEKNDLEK"
     gene            3493..4737
                     /gene="gntH"
                     /locus_tag="PKM49_06675"
     CDS             3493..4737
                     /gene="gntH"
                     /locus_tag="PKM49_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanitoxin biosynthesis MBL fold
                     metallo-hydrolase GntH"
                     /protein_id="HNY82533.1"
                     /translation="MLRAFRSDAVRKFFLVWLVGVLVFVPFITGFALQNEQPAVFVDS
                     TPFIPGEPLDKNEMRITFMGTSVIPRKGQASNSVFVETGNGDSFIFDIGSGVSTNYVA
                     MGIPYSRMDKVFLTHLHADHMSDLTTLYCFGPSLDRKTPLRLFGPSGDTPEYGTMAFA
                     EALKLLTRWHVESFSFTPTGLKSGVSGYDIDVKELPYMEVGGVAYEENGVKITHFPAV
                     HDRNGSISYKLEWNGLSMIFSGDTKPNYYMVDQGKGVDVLIHEMVVPPEVWAAKNTGM
                     KPGDVGWPQAIAYTTAVEDSSHTTQKALGYILSQANPRLGVATHFQVNEDTVDNALED
                     VRTWYQGPFAIATDLLVINVSKDRIRLRYAQVSDYSWYPSPKLYPPAQLAPPKYPSPL
                     AQLNEELLSHVIPASVYAGETK"
     gene            4903..7455
                     /locus_tag="PKM49_06680"
     CDS             4903..7455
                     /locus_tag="PKM49_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY82534.1"
                     /translation="MRFLNKVSLALYALLAILFLLSCSASTPPTPTPIPDLTDPEVFN
                     AIMTVVGVSDPSQYTREDHPFSLLPGSEVWEFSLVARQNVLYTVRFRGVRVNPVGRKY
                     VLNLRGLESGKYAVEITASSASAALKLGGAYTSASSRDSTVFFWIKVTEANPERVFYI
                     SPTGNDAGTGGQQSPWRTPHLSVAKLVPGDTLIFLDGEYVLSEFDEIIIPPSGTEDAW
                     ITLTGAPGFKPSIKGENNLYSAIILSDVSYIKISNLEFTSVTGEYFRDAINGVDGPIN
                     HIVLEDLDIHHIDEFGINFRDVEHLQVLDCRIFYCGFGAIGGPPAENDGWRHILIRDC
                     DLSYSGHYYQGVEDNPLNPYDRPDGLGLEASDGPIEIVRVTVRHNRGDGLDSKAANTF
                     IHHCLVSNNYADGIKLWGAGSKVENTLICGTGDGALESGWCPFVISYWGAPNATFKLT
                     NVTIADTPDRPSYSAHIQYDSTVPIRVTLKNCIFANAYGTVYANVNVDLIVDHCHFYR
                     PNDAVQLEAHGRTYTADQIAAGQAGVGNSVGNPLFLNPVWASDAFDYQLEGTSPAVDT
                     GTSTGAPFDDLDGTARPLGAGVDKGCYERAGSTPIGEGIVVDHLATNISSIPRNWIEA
                     VKTNSILHYARRSHGSQLNVGAEWLQSQNALYAYSENWCALPEPSNALRVWNGQTPEI
                     GDYIFPWDYWEAPEGLAVTRAILTANPSIKYSMWSWCTELDEWSAEQVDAYLAAISAL
                     EAEFPQVRFIYMTGNAQATGAEGWNRHLRNEQIRAYCRANNKVLYDFADLDCWYQGVQ
                     ATTSYAGHTYPIQHNAYASPEYEGTHVNRQSCSVKASALWWLMARLEGWGGI"
BASE COUNT         1817 a         2062 c         2065 g         1739 t
ORIGIN      
        1 aggcaactgg agaggccctc ctcgcccgaa tattccacgc aacaacgggg atgactggca
       61 gaggtggagt agggaggcac cccaccgacc gaaagcgctc actggttaaa ggtcacataa
      121 cccttttgac gaagacgttt gggaagccgt atgcgggaaa tccgcacgta cggtttgagg
      181 aggcaggaga aggaaaccgg gagagggtaa gctgccaggt ggtgtgatgg acactcacac
      241 acataaatcg agagcggtac atctctgact ccacggcgcc ttctcttgac cctacgtccg
      301 gcacgtcttt tcggctcttc gatacattac ttgaagccca ctgatcatcc aagaaatata
      361 aagtaacata agaggaataa aaagagtccg cgagatggcg gattatgcga ttagagcgga
      421 ttatcgaaac ggtctaaggg tctacagaat tattacggtt gaagatgtta attaagtacg
      481 tcttgtaacg tgtcgaagag ccgtcttttc tttttccggg agagtcctgg ccctttgcca
      541 gtttttcctc cgcgctctct gtgccctctg tgcgaaccct gttttttgat cttcggaacg
      601 aacgaaaacc ggggtatcgc gccgtcgcga aaagaaaggg cgcttacgcc gcgatgcttg
      661 tcctctccgt gttctctgtg ccctctgtgc gaaccctgtt ttttgatctt ttgaacgaac
      721 gaaaagcggg gtatcgcgcc gtcgcgaaaa gaaaaggcgc ttacgccgcg atgcctgctc
      781 tctccgtgtt ctctgtgccc tctgtgcgaa ccctgttttt gatctttgga acgaacgaaa
      841 accggggtat cgcgccgtcg cgaaaagaaa gggcgcttac gccgcgatgc ttgttctctc
      901 cgtgccctct gtgcgaaccc tgttttgctt ttaagatatg ccaacttcaa aattggcgtt
      961 tccagagaaa ggccgactgg aagtcggccc cacaaagaga aaaggggctt acgccgcttt
     1021 gactacgtgg accaaaaatc gtgcggtcga atctgacctt gtttctcgat tgcgccccat
     1081 atcttgaacc acaccggtgc tttgaagcgt agcgctttat tccgatctgg ctacgaaaca
     1141 atttcgctct tgaaacagtt ttcggaaaaa gggacagacc gtcgacccgt aacgcgcctg
     1201 ccctgcgcgc tggagtgggt ttgtaaaaca aagaccaggt tttcccgagg tagacgacgc
     1261 ctaattttca tggtaagatt ctaagcgcgc cgcgccgaac ctgggaacgc tctgggaacg
     1321 tcaacttgta agttggcatg tctttcggag tgctccgaac ttcagtccgg ctcgttttac
     1381 aggagctctt cgaacccctc gcgacactcg tttaagccgg aggcggcgct ccacgaaaag
     1441 acgcgcaaga tcgaaaaatg gagaggtgaa gaacgtggaa aaggtaaaag tcgggatcat
     1501 catctgcagc cgctaccgga attgcggagg cgggaagtgt cttagggcgg cgcgaaacct
     1561 ggaaggaggg ttcagcgtcc acgaaggaaa agaggttgaa atcgtcggat actcgtcgtg
     1621 tggcggatgc ccgggcggaa acgttgagta cgtcccggca gaaatgaaga agaacggggc
     1681 gcaatacatc cacctcgcca cggggatgat cgtgggatat ccgccctgcc caaaccttcg
     1741 gtatttcaag aggttcatag aagagaagta cggattagag gtgattctcg gaacgcatcc
     1801 gatacccgag aattactttc gaacgcactt gaacctccgc acctgggatt cgcccacctg
     1861 gcaagaaatc atcgaccccc tatctcgaga cgaggcgctc cgcaaagcct acgactgagc
     1921 cgaacaacct cgagatcgtt tatcccttgg gcgcgcctgt tagcgcccat ctgagactgc
     1981 cgaactatag ttcggcattt ttttaccttt ctgagaacgc tggacgcttg ggaacgctct
     2041 gggaccgcca acttttaagt tggcatgtct ttctggctct tcgactcgtt actcgaattc
     2101 cgctgaaaat cccagaaata cgaagcttca tacgaggaat aaaaagaaac caccaggtag
     2161 tcgatttgag cactgtgaaa ggattatcgc aacagcctga ggatctacag aattgttccg
     2221 gcagaagacg gcaattaagc acatatagaa aattgccgaa gagcccgaca aacctgccaa
     2281 tctaatgatt ggcgttccca gaaaccttgc cgaacaataa ttctgtgttc cagtacctgc
     2341 ggctttcccg gaaactctgc cgaactaaag ttcggcgttc ccatagacct gccaatctaa
     2401 agatcgaagt ttcgaaaaaa cctgtaccaa acttaggctc aagagaaggc gcgcgaagac
     2461 ggaagctatc tgcggcacgc agctaccaga ccctatctcc aaaaataggc aaaacatatg
     2521 gtatactccg tacgagaggt gatcggatgt gtctaggcag aagttcacaa tagaagaaat
     2581 agtagagatt ctttcggccg agaaaaaatc ttccgtagcg ggcgtgtgtc gaaaacacgg
     2641 aattagcgcg tccacttttt atcgttggcg ggaacgctac gcggccgctc gtgaccaatc
     2701 tggcgcatat tctttggata tcgcgaagaa gcttgtggcc ttagaggcag aaaatgccgc
     2761 tctcaagaaa gccttagccg agaaagaatt agaatatacg gtgcttaggg aattcattga
     2821 gaaaaatgat cttgaaaagt gaaggcgagt cctttgccat agccgctttc acatctcagg
     2881 atctctcatg cccataaggg tctcgaagac aaaggaacgg ttctagaaag gcgctctcag
     2941 gaactatttc tctgtgccgt ctgtgttctc cgcgcgaact ctgcttctct ttttgcgaaa
     3001 aacacctgca aatcgaaaga ctggcgttcc cgaaaaccat gccgaactaa agttcggcgt
     3061 tcccaggcgt tcccagtgtg ctcccaagca tcttttcact gtgccctctg tgttctctgt
     3121 gcgaactctg tttctctttt aacgaaaaac acctgccaat tgaaagtttg gcgttcccag
     3181 aatacatgcc gaactgaaat tccgcgatcc caggagggcg ttccgcacgg aatggtaatt
     3241 gacgcaaact ccctgtgaat ccccttcagc gaatctctat aaccccgcag aatacgcgct
     3301 taggggattg acttttcaat ttccattggc gtataatgcg gctattgggt tttcaagaaa
     3361 cagcgctgtt tcctctgtcc ccactatcat ctggttcaat taactccaag aattcgcgtt
     3421 ttcttggcca tgtctaacgt tcgcgaattt tcctctccaa tctcgtacac cttaatttta
     3481 ggaggctgaa cgatgctcag agcattccgt tcggatgctg ttagaaagtt ttttttagta
     3541 tggctagtcg gagttttggt ctttgtaccg tttatcacgg gctttgcgct tcaaaatgaa
     3601 caaccggccg ttttcgtgga ctcaactcct ttcattcccg gtgagccgct agacaagaat
     3661 gaaatgcgga tcactttcat ggggacttcg gtgattccgc ggaaagggca ggccagtaac
     3721 agtgtcttcg tggagacagg taacggggac agcttcatct ttgatatcgg atcgggcgtt
     3781 tcgaccaact acgtggcgat ggggattcct tattcccgta tggataaggt gtttttaacc
     3841 cacttgcacg ctgaccatat gagcgacctg acgaccctgt attgcttcgg accttcgttg
     3901 gatcgcaaga ccccccttag gcttttcgga cccagcggcg atactccgga gtatgggact
     3961 atggccttcg ccgaagcttt gaaactgctg accaggtggc acgtggagag cttcagcttc
     4021 accccgacgg gtctcaagag cggggtcagc ggttatgata tcgatgtaaa ggagctgccc
     4081 tatatggagg tcggtggcgt cgcctacgag gagaacgggg tcaagatcac acattttcca
     4141 gcggtgcacg accggaacgg ttccattagc tacaaactcg aatggaacgg cctgtctatg
     4201 atcttctctg gtgacacgaa accgaactac tacatggtcg accagggaaa aggggtggat
     4261 gtgctgattc acgagatggt cgttccgccg gaggtgtggg cggcgaaaaa cactgggatg
     4321 aagcccggcg acgtggggtg gccgcaggcg atcgcataca ccaccgcagt cgaggactcc
     4381 tcacacacga cccagaaggc gctcgggtat attttgagcc aagcgaatcc ccgccttggg
     4441 gtggccacgc acttccaggt gaacgaagac acggtggata acgcgctgga agatgtccgc
     4501 acctggtatc aagggccctt cgccatcgcc acggaccttc tggtgatcaa tgtctccaaa
     4561 gaccgcatac gcctgcgcta cgctcaggtc tcggattaca gctggtatcc gtcgccaaaa
     4621 ctgtatcctc ccgcccaatt ggctcctcca aagtatccgt cgccgctcgc ccagctcaac
     4681 gaggagttgt tgtcgcacgt cattcccgcg agcgtctacg caggagagac caaataatcg
     4741 cgcattcaag ttgaaaaacc cgttcctcct gccgccttta gggtatagta gggtagttga
     4801 gcgttcggct accggaatcg aagcgccccg caacaacacg aaaatggggc gctcccgtgt
     4861 ttcgtagcat catcgttttc gaacaaaaaa ggaggtacgg gtatgagatt cctcaacaag
     4921 gtatcgttgg cgttatatgc gcttttggca attctgtttt tactttcctg cagcgcttcg
     4981 acgccgccaa cacctacgcc gataccggat ttgactgatc cggaggtctt taacgcgatt
     5041 atgacggtcg tcggagtctc agacccctcc caatacaccc gggaagacca tccgttctcc
     5101 cttcttccgg gttcggaggt ttgggagttt tccctcgtcg cacgtcaaaa cgtcctctac
     5161 accgttcgtt tccgaggcgt ccgggtcaac cccgtcggta ggaagtacgt cctcaacctg
     5221 agaggtctag agagcgggaa gtacgctgtc gaaataacag cttcgagcgc gtcggccgcc
     5281 ctgaaattgg gtggggcgta tacgtcggct tcttcccgcg attctacggt tttcttttgg
     5341 ataaaggtca ccgaggcgaa tccggaacgg gtcttctata tctctcccac cggtaacgac
     5401 gccggaacag gcggccagca gtcgccttgg cgaacgccgc acctgtccgt tgcgaaactc
     5461 gtaccagggg atacgttgat ttttcttgat ggagagtacg tcttgtcgga gttcgacgag
     5521 atcataattc ctccctctgg aacggaagac gcttggatca ctttaacggg cgcgcccggc
     5581 ttcaaaccgt ccatcaaggg tgagaacaac ctgtattccg cgatcatcct ctccgatgta
     5641 tcgtatatca agatatcaaa cctcgagttc acttccgtta ccggtgagta cttccgagat
     5701 gccattaacg gggtggacgg cccgattaac catatcgtcc tcgaagactt ggatattcac
     5761 catatcgacg agttcgggat caattttcgt gacgtcgagc atctccaggt tctggactgc
     5821 cgcatctttt attgcgggtt tggggcaatc gggggcccac cagcggagaa cgacggctgg
     5881 cgacatatct tgattcgcga ctgtgacctc tcctacagcg ggcattatta tcaaggcgtt
     5941 gaagacaacc cgctcaatcc ttatgatcga cccgacggcc tcggcctcga ggcttccgac
     6001 gggcccatag agatcgtccg tgtcaccgtc cggcataacc gcggggacgg tctggactcc
     6061 aaagcggcga acaccttcat ccatcattgc ctcgtgtcga acaactacgc cgatgggatt
     6121 aaactgtggg gcgccgggtc gaaggtagaa aacaccctca tctgtgggac cggcgacggc
     6181 gcgctcgagt cggggtggtg tccgtttgtg atcagctatt ggggtgcccc aaacgcgaca
     6241 ttcaagctga cgaacgtcac gatcgcggat accccggacc ggccaagcta tagcgcccac
     6301 attcaatacg attcgacggt acccatccgg gtgaccctta aaaactgcat tttcgcgaac
     6361 gcctacggga ccgtttacgc gaacgtgaac gtggacctca ttgtcgatca ctgtcacttt
     6421 tatcgtccaa acgacgccgt tcaactcgag gcgcacggta gaacctacac cgccgatcag
     6481 atcgccgccg ggcaagcggg cgtcgggaac tccgtgggca atcccttatt cctgaatccg
     6541 gtttgggctt ctgatgcgtt tgactaccaa ctcgaaggaa cgagtccggc cgtggatacg
     6601 ggaacctcta ccggagctcc gtttgacgat ctcgacggaa cagcccgccc tttgggcgcc
     6661 ggagttgaca aaggctgcta cgagcgcgcc gggtcgacgc caatcggaga agggatcgtc
     6721 gtcgatcatc tggccacgaa tatatcttcg atcccgagaa attggatcga ggcggtaaaa
     6781 accaattcca tccttcacta cgcgaggagg tcccacggct ctcagctcaa cgtgggcgca
     6841 gagtggctcc aaagccagaa cgcgctctac gcatacagcg agaactggtg cgccttgccc
     6901 gagccctcga acgcattacg cgtctggaac gggcagaccc ccgagatcgg cgactacatt
     6961 tttccttggg actactggga ggcccctgag gggctcgcgg taacccgcgc catcctcacc
     7021 gcgaatccgt cgatcaaata ctcgatgtgg tcgtggtgca cggagttgga cgagtggtcg
     7081 gcggaacagg tggacgccta tctcgcggcc atttccgcgc tcgaggcgga gttcccgcag
     7141 gtcaggttca tctatatgac aggcaacgcg caggccactg gcgcggaagg ctggaatcgc
     7201 cacctcagaa acgagcagat tcgcgcttac tgccgggcca acaacaaagt cctatacgat
     7261 tttgcagacc tcgactgctg gtatcagggc gtacaagcga cgacctccta cgccggacac
     7321 acctatccga ttcaacacaa cgcctacgcg agtcccgagt atgaggggac gcacgtcaac
     7381 cgacagagct gctcggtcaa agcgagcgcc ctatggtggc tgatggcgcg cttggaggga
     7441 tggggcggta tttgagaggg gcattgcgat acgaaacgcc gttagacccc ggacctctga
     7501 aaaagaaccg cttgagtctt atgcgtacga aaccaacttt ggcgagatag atagattact
     7561 cccgtgcgag tggaacagcg tgggtatgct atcgaggtac aggaaaaaaa gaggaggaag
     7621 tccgcctttt gctcttttta taccgcgata ggcttgggga gttatcgcgg ggtaaccagc
     7681 ggg
//
LOCUS       DAOPXN010000104         7676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9534_length_7676_cov_5.240782, whole genome shotgun sequence.
ACCESSION   DAOPXN010000104 DAOPXN010000000
VERSION     DAOPXN010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9534_length_7676_cov_5.240782"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="PKM49_06685"
     CDS             complement(<1..684)
                     /locus_tag="PKM49_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82535.1"
                     /translation="MEAGAYETIEIQGDPVYKILSIEPTEVFEGVTFFSLGSVGYLSL
                     LEEYGYVLAAKAKALGVSGAIIGRTKPATKATAGDGRYLSSSPYLTAEALKCIAKGLL
                     IGGIFPIVDLEQGYDLDVLKALVSRETYIGLLCRSQTLSNDALLSDFLSRNNWFPILL
                     DSGLEDTTAITHTFFWDFPLSDEKREEIRKNLLLMAIVQVKKPPFSTEIQVIHNNQTA
                     VKPGISYVVI"
     gene            complement(632..1519)
                     /locus_tag="PKM49_06690"
     CDS             complement(632..1519)
                     /locus_tag="PKM49_06690"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HNY82536.1"
                     /translation="MGGSALSVRYEAAILDIDGTLIDDRLRLSDRTKAAIQTLADNGV
                     TLVLCSGRMYLSMKRWVERHFERHFHMISYNGGQIHLAGEKDPFYSRRFSFETSLEIL
                     NALKKAGSYRQYYWNERIFSSPPAKWAELYALHSGVDVAVQEDLERTAEETRVEPVKI
                     LAIDTPDRVDRLQSAFAGKIAGANVFKSFETYLDFVPAGTNKGVAIREIALKLNFSLE
                     KTVMLGDGENDSFGFEVVGYPIAMGNAQRALKDRAAWIAPDNNREGAFWALVHAFPDF
                     FPDGSGRLRNDRDSGGSRL"
     gene            complement(1501..3483)
                     /gene="dxs"
                     /locus_tag="PKM49_06695"
     CDS             complement(1501..3483)
                     /gene="dxs"
                     /locus_tag="PKM49_06695"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNY82537.1"
                     /translation="MESFLTNSSNLIPRIKTASSSELEQLASEVRSRIFEVVSKNGGH
                     LSSNLGTVELTLALYRQFDFPEEDILVWDTGHQAYTHKLITGRAERFDTLRQMGGISG
                     FTSRFESPYDSYGAGHVGTGVGAGLGFEQVLRKEKREGNVVVVLRDGALTNGCALEAL
                     NQASNLNSYLRVVINDNGMSIGENVGSFARSFSLLRTNPKYATVKQKIKSFLTNTGLE
                     PFETILERIRDSMKSYLVPQNLFEAMGFKYIGPVDGHDIDLLSRVFYNLKTDFKKPAI
                     IHVITQKGKGKGWSESQPTRYHGVGPFHLDDGTLVASSKTISFSQAFGMTLTALAARD
                     PKVIAVTSAMADGTGLSPFRERYPDRFFDLGITESFSTLFTGVFALKGWHPVFAVYST
                     FLQRAYDALVHDIALQRVGAVFCVDRAGLVGDDGPTHHGVFDLSFLLTLPGATIYNPR
                     SIRELVSVLKCIVTDRWPSEGPVFIRYPKKAEELTEEHVRDWIEELETDDVLRWGTVA
                     QQLDSDQEGRTPETLLFSTGAITQTAIAAAPALGTSYRVIDAIRAKPLDWETIDLFFS
                     SSSSKRVVTIEEGTTVGGFGQYLTLELLRRYGDRIEGFLNIGLPDAFIEHGKNEHLYR
                     RYGLDVDGLAAQFRVFSKKMDTDPSGSLAGLWGDRR"
     gene            complement(3419..3700)
                     /gene="xseB"
                     /locus_tag="PKM49_06700"
     CDS             complement(3419..3700)
                     /gene="xseB"
                     /locus_tag="PKM49_06700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNY82538.1"
                     /translation="MKNENETDTQSIEEIFTKIETLIDELESGKEPLDTVLAKFKEAV
                     GLYQKASGLLRDAKVKVTEMMRELEGEKDGELPNELVEPDTTDKDGFVL"
     gene            complement(3703..4980)
                     /gene="xseA"
                     /locus_tag="PKM49_06705"
     CDS             complement(3703..4980)
                     /gene="xseA"
                     /locus_tag="PKM49_06705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNY82539.1"
                     /translation="MDVYSVGQCLMILQLALARDEVLQGSLTVRGEVSGWKVVRGHAY
                     FSLKDPGGLIKSVYFSVPPSLAGKIAEGNVVDATGQFKIYAERGELQFYVRTIRLQSA
                     AGLLQLRFEELKTRLNREGVIPKPPEEKRQLPRFPRRIGVVASRSSAAFADIEKTLQK
                     RYPVAEILLFHTGVQGEIAKMEIVRALRQASQSEADVVILARGGGSIEDLWAFNEEVV
                     VRAVRGCGKPIIVGVGHEIDHTLSEYAADVIASTPTAAAMQASPDLRASMEHYRQKVD
                     HARRSLLLHIHRIEEKARSFARLLRFYRPDQTLLREMQNARRIRHRLASAGKSIGQAI
                     DRTLEDHFRRILSVSPVRRLEDIGTRLDALYRIVKGFDPSLVLHRGYAWIEVDGKIVS
                     SVVKFGENDPFTVRLKDGTLEAITKRTNPSGAE"
     gene            5047..5286
                     /locus_tag="PKM49_06710"
     CDS             5047..5286
                     /locus_tag="PKM49_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HNY82540.1"
                     /translation="MDKSITVYSTPLCPYCVMAKKFLKEAGYAFKDIDVSRDANAAER
                     MVRKSGQRGVPVLEIGSEVIVGFDKARIKRALGIL"
     gene            complement(5304..7094)
                     /locus_tag="PKM49_06715"
     CDS             complement(5304..7094)
                     /locus_tag="PKM49_06715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNY82541.1"
                     /translation="MKTKTVALKRFVLWWTILYCAFLVILGFFFGSYYSGLMKARTLS
                     IVSAFPLTFDSLLKERTDQDPHRAPRSYAYWKWDEKGSLVSSSVDQTVDGPFIPPENY
                     DKIKLLTKNANPCLSDFVRKPPQGPLYFYYALAEGETLTVFAFLAEEWLTRLKSLLPP
                     VESGFVADRNGNGFYFDAKGIEEVNSADPATYRSMGSVMVKYLGEHYFYSSASFSNLT
                     LTRLLPFYRTIPFLFLTAIIPFLGGLIVLWGVSHHIKRLQREQASAFRRLSQEMDRRA
                     FGQGAAARYGPNDPATELREKIREIVSENAEYEKEIDRLTRKSALLSESIRDSYQSVS
                     SVNRLLQKFAFSEDFTFPRAIDLVTNVILSTDQAVEKAVVYLNDEIAFQKGTISIPEN
                     GSEEFSIEQGHNRIRVLLEPSKECTERDFSIKQEVLLTFLYTTAILFQLKRETQLDPF
                     TGLYRFEHFSRLVQVELDKAQRYRRCCSLAMTNLRNFRIINERYGLNTSNRFLATVSK
                     VIRENIRSGDLAGHYSGDRFLIFFQEIVKNDSRKKMEKLKELMMQALPKDVHPGQWDF
                     EYGIADCLEGSLSFTELVTTAYNDMAAKRE"
     gene            complement(7091..>7676)
                     /locus_tag="PKM49_06720"
     CDS             complement(7091..>7676)
                     /locus_tag="PKM49_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82542.1"
                     /translation="VHRRADAYRCRPPSVGVDFQWVPVEEAVKWSLDGVSAPTPPSSS
                     DALFLFLCEPEWVTQIARAWEFSQFDRVILGPWSIPVDTFYTDTRTLAGIRFLLKPSL
                     GTEAAYSWAEPVYPWVTYVVEASKAVFSDKGQSGVKPSADWFSRSARAISLLPQEQRN
                     AQSEAVWTLWEYTASGSLLKKRYDPITQGWEGCP"
BASE COUNT         1755 a         2154 c         1994 g         1773 t
ORIGIN      
        1 tatcaccaca tacgagattc ctggttttac cgcggtttgg ttattgtgta tcacctgaat
       61 ctccgtggag aagggtggtt ttttcacctg aacgatcgcc atcagtagca ggttttttcg
      121 tatttcttcc ctcttctcgt cggaaagcgg aaagtcccaa aaaaaggtat gggtgatggc
      181 agtcgtatct tccagtccgg agtccagaag aatcgggaac caattgttcc tggacaaaaa
      241 atccgacaac aatgcgtcgt ttgagagagt ttgcgatcga cacagcaaac caatataggt
      301 ctcgcgcgaa accaacgctt tcaacacatc caggtcatag ccttgctcta aatcgacgat
      361 agggaagatg cccccgatca aaaggccttt ggcgatgcat ttcagcgcct cggcggttag
      421 gtacggagaa gaagagagat agcgcccgtc gccagcggtg gctttcgtag ccggtttcgt
      481 ccgcccgatg atcgctccgc tgactcccag tgctttcgct ttggcggcca aaacgtatcc
      541 gtattcttcc aacaaagaaa gatatcctac ggaccccaaa gagaagaagg tgaccccttc
      601 gaagacttcc gtgggctcta tcgaaagaat tttataaacg ggatccccct gaatctcgat
      661 cgtttcgtag gcgcccgctt ccatcgggga aaaaatcagg aaaagcgtga accaaagccc
      721 aaaaagcccc ctcccggtta ttgtccggag cgatccaggc cgcccgatcc ttgagagcac
      781 gttgcgcgtt gcccatagca atcggatacc ctaccacctc aaacccgaaa gaatcgtttt
      841 cgccatcccc tagcatgact gtcttttcga gcgaaaagtt caattttagc gctatttcgc
      901 gtatcgcgac ccctttgttc gttcctgccg gtacaaaatc caaataggtc tcgaaggatt
      961 tgaagacgtt cgcacccgct atcttacctg cgaaagcgct ttgcaaccga tcgacgcggt
     1021 ctggcgtatc gatcgccagg atcttcaccg gttcgaccct ggtttcttct gccgttcgtt
     1081 ccaaatcctc ttgaacagcg acgtccacac cggaatgaag ggcgtaaagc tcggcccact
     1141 tagcgggcgg agaggagaag atacgctcgt tccagtagta ttgccgatag ctgcccgcct
     1201 tcttcaaagc gttcagaatc tctaaagaag tttcaaaaga gaaccgccta gagtaaaagg
     1261 gatccttctc tcccgccaag tggatttggc cgccgttgta agaaatcata tgaaagtggc
     1321 gttcgaagtg ccgttccacc catcttttca tagacaggta catcctgcct gaacagagaa
     1381 ccagggtcac gccgttgtcg gcgagggttt gtatcgccgc tttggtccgg tcggaaaggc
     1441 gcagccggtc gtctatcagg gtgccgtcga tgtccaatat cgccgcttcg tacctgacac
     1501 tcagcgccga tccccccaaa ggcccgccaa agaacccgac gggtctgtgt ccattttctt
     1561 cgaaaacact cgaaattgtg cggccaatcc atcgacgtct aagccatagc gacggtatag
     1621 atgttcgttt ttcccgtgtt cgataaaagc gtccggaagt ccgatgttga ggaagccttc
     1681 gatccggtct ccataacggc gcaagagctc cagggtcaaa tactgtccga acccccccac
     1741 ggtcgtcccc tcttcgatgg tcaccacccg ttttgaagag gacgaagaaa agaagaggtc
     1801 gatggtctcc caatccagcg gtttcgcgcg tatggcgtct atcacacggt aggaggtccc
     1861 cagcgcggga gccgcagcga tggccgtttg cgtgatcgca cccgtcgaaa aaagcaaggt
     1921 ctccggcgtt cttccctcct gatctgagtc gagctgttgc gcaaccgttc cccatcgaag
     1981 gacatcgtcg gtttcgagct cctcgatcca gtcccgcacg tgttcttcgg ttaactcctc
     2041 ggcttttttg gggtatcgga tgaagacggg accttccgag ggccaccggt cagtcacaat
     2101 gcatttcaat accgaaacga gttcacgtat gctcctcgga ttgtagatcg tggcgcccgg
     2161 taaggtcaac agaaaactca aatcgaaaac cccgtgatgg gtcgggccat cgtcgccgac
     2221 caaccctgcg cgatccacgc aaaagacggc ccctaccctt tgcaatgcga tatcatgcac
     2281 caaagcgtca taggcgcgtt gcaaaaaggt cgaatagacg gcgaagaccg gatgccagcc
     2341 tttcagcgcg aagacaccag tgaataaggt cgagaaggat tcggtgatcc cgagatcgaa
     2401 aaagcgatcc ggataccgtt cacgaaaggg agacagcccc gtcccgtccg ccatcgccga
     2461 ggtgaccgcg atcaccttcg ggtctcgggc ggctaacgca gtgagcgtca ttccgaaagc
     2521 ctgactaaag gaaatcgtct tcgaagaggc cacgagcgtc ccgtcatcca ggtggaaggg
     2581 cccgaccccg tggtaccgtg tcggttgaga ctccgaccag ccctttccct ttcccttttg
     2641 cgtgatcacg tgaatgatgg ccggcttctt gaaatcggtc ttcaaattgt agaagacgcg
     2701 tgacaacagg tcgatgtcgt gcccgtctac cgggccgata tatttgaacc ccatcgcttc
     2761 gaacaggttc tgcggcacga ggtaagactt catgctatcg cgtatccttt ctaaaatcgt
     2821 ttcgaaaggc tccagcccgg tattggtcag aaaactcttt atcttctgtt tcaccgtcgc
     2881 gtacttgggg ttggtccgta aaagcgaaaa gctacgcgcg aagctgccaa cgttttcgcc
     2941 gattgacatc ccgttgtcgt tgatgaccac cctcagatag gagttcagat tcgacgcttg
     3001 gttgagcgcc tctaacgcgc aaccgttcgt caaggcgcca tcccttaaca ccacgaccac
     3061 attgccttct cgtttttctt tccgtaatac ttgctcaaat cccaaccccg ccccgactcc
     3121 cgttcccacg tgaccggctc cgtaactgtc atacggagac tcgaagcgag aggtgaaccc
     3181 gctgatcccc cccatttgcc gtaaggtgtc gaacctttcg gcgcgcccgg tgatcagttt
     3241 gtgcgtgtag gcctgatggc ccgtatccca aacgagtatg tcttcttccg gaaaatcgaa
     3301 ttgccggtag agcgctaagg tcagttcaac ggttccgaga tttgaggaga gatgcccgcc
     3361 gtttttcgag accacttcga aaatgcgaga ccgaacctct gacgcgagtt gttcgagttc
     3421 agaggacgaa gccgtcttta tccgtggtat caggttcgac gagttcgtta ggaagctctc
     3481 catctttttc accctccagc tctctcatca tttcggtcac tttgaccttg gcgtcgcgaa
     3541 ggagaccgga ggctttctga tagagtccca cggcttcctt aaatttcgcc aaaaccgtat
     3601 ccaaaggttc ctttcccgac tccaactcgt cgatcaaggt ctctattttc gtgaatatct
     3661 cttcgatgct ctgtgtgtcc gtttcgttct cgtttttcat cgttattccg ctcctgatgg
     3721 gttcgttctt ttcgtaatag cctctagggt tccgtctttg agcctgaccg taaacgggtc
     3781 gttttcaccg aattttacca cggaagagac gatttttcca tccacctcga tccaggcgta
     3841 gcctcgatga aggacgaggc tcggatcgaa tcccttgaca atgcggtaga gcgcgtccaa
     3901 ccgcgtcccg atatcctcga gccgccgtac gggcgacacg gaaaggatac ggcgaaaatg
     3961 gtcctccaga gtgcgatcta tggcttggcc tatgctcttc cctgcagagg ccaagcggtg
     4021 tcgtatccgc cgcgcatttt gcatttcacg caaaagggtt tggtccggcc ggtaaaatcg
     4081 cagcaaacgg gcgaaactcc gcgccttttc ctcgattcga tggatgtgaa ggagtaagga
     4141 ccgacgcgca tgatccacct tctgccggta atgttccatc gacgcgcgca ggtccgggga
     4201 cgcttgcata gccgcggcgg tcggggtgga agcgatgacg tccgccgcgt attccgagag
     4261 cgtatggtcg atctcatggc caacgcccac aatgatcggt ttgccgcatc ccctcaccgc
     4321 gcgaacgaca acctcttcgt tgaaggccca taggtcctcg atggaacccc ctcccctggc
     4381 gaggatcacc acgtctgcct cgctctggga cgcctggcgc agggctcgca cgatctccat
     4441 ttttgcgatc tctccttgaa cgcccgtgtg aaacaacagg atttcggcca ctgggtagcg
     4501 cttttgcagc gttttctcga tgtcggcgaa ggcggcggaa ctccgtgagg cgacgacccc
     4561 gatcctacgc ggaaaacgcg gaagctgtct cttttcttcg gggggcttgg ggatgactcc
     4621 ctcccggtta agtcgcgttt tcagttcttc aaaccggagc tggagcaacc ccgctgcgct
     4681 ttgtaaccgt atcgtccgaa catagaactg tagctctccc cgctcggcgt atatcttgaa
     4741 ctgccccgtc gcatccacca catttccttc ggcgatcttg cccgccaaag acgggggtac
     4801 agagaaatat acggatttga tcagcccgcc ggggtctttc aaggaaaaat aggcgtggcc
     4861 gcgaacgacc ttccagccgg agacctctcc acgtaccgtt agcgaaccct gtaacacctc
     4921 gtctctcgct aaagccaact gcaagatcat caaacactgg ccgacgctgt agacatccat
     4981 aggcttgaca tacccccatc cggtatatta taatagggcg aaaagacaaa aaaaggagtg
     5041 acacagatgg ataaatccat aaccgtttat tcgaccccgt tgtgccctta ttgtgtaatg
     5101 gcaaagaaat tccttaaaga ggccgggtac gcgttcaaag acatcgacgt ttcccgtgac
     5161 gccaatgctg ccgagcgaat ggttcgaaag tccggccagc gcggagtccc agttttagaa
     5221 atcgggtcgg aagtgatcgt cggcttcgac aaggcccgca ttaaaagagc cttgggtatc
     5281 ctctgaagcg tcgtcccggc agattattct cgtttggccg ccatatcgtt ataggcggtc
     5341 gtaaccagtt cggtaaagct taaagaacct tccaggcaat cggcgatccc gtactcgaaa
     5401 tcccattggc cgggatggac gtcttttgga agggcttgca tcatcaattc tttcagtttt
     5461 tccatttttt tcctgctgtc gttcttaacg atttcctgga agaagatcaa aaatcgatca
     5521 cccgaatagt gcccggccaa atctcccgac cggatattct ctcgtatgac tttggacacg
     5581 gttgcgagga aacgattgga ggtgttcagt ccgtaccgct cgttgatgat ccggaagtta
     5641 cgaagattcg tcatcgccaa agaacagcat cgtctgtacc gttgcgcttt gtccagctcg
     5701 acctggacga gccgagagaa gtgctcgaat cgatacaagc cggtaaaggg gtccaattgc
     5761 gtctcgcgct taagctggaa taatatggcg gtggtataca agaaggtgag caacacctct
     5821 tgtttgatcg aaaaatcgcg ttcggtgcac tccttcgagg gttctaacaa cacgcggata
     5881 cggttgtgtc cctgctctat cgaaaactcc tcagacccat tttcgggtat cgatatagtc
     5941 cccttttgaa aggcgatctc atcgttcaag tacacaaccg ccttttccac ggcctgatct
     6001 gtcgagagga tcacgttcgt gaccaaatcg atggctcggg ggaaggtgaa gtcttcggaa
     6061 aaggcgaact tctgcagtag cctatttacg gaagagacgc tttgatagct atcccgtatg
     6121 gattcactca ggagggcgga tttccgagtc aaacggtcta tctctttttc gtattccgcg
     6181 ttttcggaaa cgatttctcg tattttctct cgaagctccg tagcgggatc gttcgggccg
     6241 taacgagcgg cggcgccctg gccgaaagcc cgccggtcca tttcttgaga caacctccga
     6301 aaagcgctcg cttgctctct ttgtagtcgt tttatatgat ggctcacgcc ccagagcacg
     6361 ataagtccac ccaaaaacgg tatgatcgcc gtgaggaaca ggaaaggtat cgttcggtaa
     6421 aagggcaaca gacgggtcaa agtcaaattg gagaaagacg cggagctgta gaagtagtgt
     6481 tctcctaaat acttaaccat cacggacccc atcgatcggt aggtggcagg atccgcagag
     6541 tttacctctt cgatgccttt cgcgtcaaag taaaaaccgt tcccgttccg atccgcgacg
     6601 aacccgctct ctaccggagg aagcagggac ttcaagcggg tcagccattc ttcggcgagg
     6661 aaggcgaaaa cggtgagggt ttctccctcg gcaagcgcat agtagaaata gagaggtcct
     6721 tgggggggtt tgcgcacaaa atcggagaga caaggattcg cgttttttgt gaggagcttg
     6781 attttgtcgt agttctccgg agggatgaag ggcccgtcca ccgtttggtc cacgcttgat
     6841 gaaaccaaag accccttttc atcccacttc cagtatgcgt acgatcgcgg cgccctgtgc
     6901 gggtcttgat cggtcctctc tttgaggaga ctgtcgaagg taagaggaaa ggcgctcacg
     6961 atagacagcg tccgggcttt catcaacccc gaataatagg agccgaaaaa gaagccgagt
     7021 atgaccaaaa aggcgcagta caggatcgtc caccacagca cgaaacgctt caatgctacg
     7081 gtcttcgttt tcaagggcac ccctcccaac cctgcgttat cgggtcgtaa cgttttttta
     7141 atagactccc actggcggtg tattcccaca acgtccacac cgcctccgat tgagcgtttc
     7201 gctgttcctg cggaaggagt gagatggcgc gggcggatcg ggagaaccag tcggcggaag
     7261 gcttcacccc gctctgaccc ttatcggaaa aaaccgcttt cgaggcctct accacgtagg
     7321 tcacccaagg ataaaccggc tctgcccatg aatacgcggc ttccgttccg agcgagggtt
     7381 taaggaggaa acggataccg gccaacgtac gcgtatccgt gtagaaggta tcgaccggaa
     7441 tgctccaggg tccgaggatg acgcgatcga attgcgagaa ctcccaagct cgcgcgatct
     7501 gtgtgaccca ctccggctca cacaggaata gaaagagtgc gtcggacgac gaaggtggag
     7561 tgggggcact cacaccgtcg agcgaccatt tcacggcttc ttcgaccgga acccattgaa
     7621 agtccacacc gaccgacggc gggcgacacc gatatgcgtc ggcccggcga tgaacc
//
LOCUS       DAOPXN010000105         7513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9818_length_7513_cov_4.899571, whole genome shotgun sequence.
ACCESSION   DAOPXN010000105 DAOPXN010000000
VERSION     DAOPXN010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9818_length_7513_cov_4.899571"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKM49_06725"
     CDS             <1..404
                     /locus_tag="PKM49_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol carrier protein domain-containing
                     protein"
                     /protein_id="HNY82543.1"
                     /translation="LVHSYWQNGEKIEWTEMSSRMMRVVNIPALLVSLADQDALARCP
                     FGIVDPWLEVPEPYRPGPDKWTMSVATLAQLAAGYRSATHLYRQGMILEPGETEDAPR
                     DRIWETADAPAVVKELERLFPPVTPFQTEKW"
     gene            699..1088
                     /locus_tag="PKM49_06730"
     CDS             699..1088
                     /locus_tag="PKM49_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNY82544.1"
                     /translation="MKRNGFTLVELLIVMAVIAALISVVAPMGLNALKQARTVQVATD
                     LRAAMNAAIGKVAAYGKFVTANTIDVATGYKTAMTIAAGSSNTYTISVTGWGDGSKYD
                     VDYLKAAFDNAFAGGNLTVDVSSYIYN"
     gene            1279..>1782
                     /locus_tag="PKM49_06735"
     CDS             1279..>1782
                     /locus_tag="PKM49_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82545.1"
                     /translation="MFSLVVFVCLYRLLKKRRFAFEAPPLLLGILCAFALFLIPAIPL
                     QNERALLFFGIVFFIIIGASVFRFEDFSVAAVQKWTVLSALWISFIGIQNFYGITFFN
                     IYVPSAFDRSSIIATVGNVNYVSNYLASILPITIAAYLTMEDGRWRFLAFIATVAFSS
                     HALDWAMA"
     assembly_gap    1783..1792
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1793..3672
                     /locus_tag="PKM49_06740"
     CDS             <1793..3672
                     /locus_tag="PKM49_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNY82546.1"
                     /translation="RDRLPGVWASAVAAAVTVLWGQTRSVYLGLFVAFLVFFSLLGTS
                     DRRHLITKRKLAGMVLGVVIILALYAFPPGVPENRRPLRLSVSRAQELQLPYDEATGS
                     LYRRVFEWKTALEMFTHSPLYGWGWGSYILLSQDFQVKVTEKDPAYFGFYEKSAEAHS
                     DFLQMLAETGIIGFGVWIALLLYIGILGVKRWLATKNLMILAALSGWLMILVHALTEF
                     PLHMMPSAGIFAVFSGFLVSEGKRKTFPRAVGLAFLFLTLFFSFIALKTALADSFYAY
                     GIYQREKAQNQYLKDMESVGRAIVLSSKGSEETPEWLEDAIRKEKAAAAERLSSSYYS
                     QYLFFTNALIADPGLSSATYEIATLIGKMEELVPRPPFLLFDFPPFRYTGVSALREAP
                     TEYPELGRWVFKLKVQERERIEYLYRYFRGLCLSINSMIDPAVYLNIGRSANEMLVLY
                     EEWDVEEPEERALWLTWMLYGYEKAFRLNGARQYTEDLELDHLDLEYLDAVIRHGVDV
                     EERVTEVLGFRRRLAQHTLKKDWRFPKKWYNYFVEKMDDGYFAGRPTYRDRFIEVFEE
                     YARRYREMEGYFREMDNALQSKETKISAADRYELYRDMKDIERFLEDFERRFSNGAAE
                     G"
     gene            3675..4076
                     /locus_tag="PKM49_06745"
     CDS             3675..4076
                     /locus_tag="PKM49_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNY82547.1"
                     /translation="MRRAFTIVELLIVLGIIALLTSVVIKVSADAVIHARAVQVAVNL
                     TAIMRAAQSRLLTNSATTADLADLDIDLEFPDDYALDMVYRDSDSSVEVKVSYTGIVS
                     KSHISGLLAPDATVSETENEIAYSKVFPVYW"
     gene            4311..>7513
                     /locus_tag="PKM49_06750"
     CDS             4311..>7513
                     /locus_tag="PKM49_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82548.1"
                     /translation="MRSKRFYSLPVFLLVLLLALIGCFPVVKPDKTPPVVETPVITLS
                     ATTVPDTGGGMTVTVSVAAQDAHSNLKDAVITLTWNTVGTDLRDRGSVTKTVAYPAAK
                     TGTATAAFSVNLLGSDITLPVGIYSFSASAVVQDVKLNKAEPKSATAAQNLTVQKSSE
                     HSPTINVTYTPTSGGKVGIPFTATVVGADTDGDLTYLEFYILSDTATVVSRMATSTGT
                     TSLTITTEQITLAMLGMGPGDFSKTLYWYALARDAQGHVTQYTNKSITAQRMDLAVIE
                     SIGHVRTPSEPNMPSHYRVLAGATPPAISELQEDQRLPMQFGVSKMRINLMDSIVLAG
                     YKARGYIFTTTSEIFDATPDVWRAEGAVGADKMLYLDMVNFWRQVDLDTSKWFVLEIL
                     NNAGTPIVRYIWNIKALGDTFEPMVSIIDSGKDYREGVPLEFTICATDEFLEAITDLR
                     VRVNIGQKGWRDLEDVVSDMNPAVDQKEGWLFAVRYHVKVQVFPGLTNYASGLVDDGV
                     DYIVSNAIASLNGTGAHQNAKWFDVFHGKIPGMPTTAAGRSEYWWKTDGYGHILAEDD
                     DNGHWPGNRTPDEEPYDPTPSHNTDELDHRALPKWSYVYEFTDLDTTRVNEIRKEAFV
                     ENTTFKVWIYPEVVRVNRADIEAGVTKPEVLDVVDVSIGPGTWRDQHVDYQMQIEIAD
                     WRWGGENPDIGKVSQSANFTIDEDEETPRIQISGSYSDDPSQSFKHLFKDQWQWYSPV
                     KNYAVPNFFVHELEVFTGASITIKAQDDIALHDLLVFYDSENYTTCVSKIDSPKADYI
                     GRNDAFSHPLTGFDYVYDEGMLAYRLIKGKETTGEATLVPLVYQSLPATPKAFPIEEP
                     RILRILGKDFKINGKIISPIFTLDKNYEYYVNFTNDDVVDSWAIAAHQNSFGRENDII
                     RANPVDKEYETYAGEYSTVFLGSKLNTPGTKEDQKAEFTFRVPDVTGDYWLWIVARDR
                     SAQDTKLFDYPFDERHDYKEDTYLGEYPSYATKNRQCEIFGTWFDDGSPNFDKMYYDD
                     SMSEDDADMVILLRLRIKSHSWDIVRLDIHEPDLVSYP"
BASE COUNT         1776 a         2041 c         2011 g         1675 t
ORIGIN      
        1 gcttggtaca ctcctactgg caaaacgggg agaagataga gtggacggag atgagctcac
       61 ggatgatgcg cgtcgtcaac atccccgcgc tcctcgtgtc tttggcggat caggatgcgt
      121 tagcccgatg ccccttcggc atcgtcgacc cgtggctgga ggttcccgag ccctaccggc
      181 cgggaccgga caagtggacg atgagtgtcg cgacgttggc gcagcttgct gccggatacc
      241 gatctgccac ccacctatac cgacagggaa tgatcttgga gccgggggaa acagaagacg
      301 cgccccggga tcggatatgg gaaaccgctg acgcgcccgc cgtcgtgaag gaactggagc
      361 gcctcttccc gccagtcact ccctttcaaa cggaaaaatg gtaaacgagc gcggttccac
      421 cacattcaag aaactgagat ttgatccgat aaggtatcag cgatcctcaa gagcggtttc
      481 cggtaacagg aacaacggaa taactacgga atcagaacta attttgacaa aacgaaataa
      541 tcgtggtaga attgcttagt ttatcgttta cccatctttt gttataaaca aactttttat
      601 gatttagact cagcaatgaa acgggataag tttgtgtggt tattcaagta cggcagtttg
      661 ggcgcgaact caaaaataaa tttaggagga gtgagactat gaagagaaat ggatttacgt
      721 tagtggaatt actgatcgtt atggccgtca tcgcggcgct gatttcggtc gtcgccccga
      781 tgggattgaa cgcattgaaa caggcgagaa cggtgcaagt ggcgacggat ttgagagcgg
      841 cgatgaacgc tgccataggg aaagtcgctg cctatgggaa attcgtaact gcgaatacta
      901 tcgacgtagc aaccggttac aaaaccgcta tgacgattgc ggccggatcg tcaaatactt
      961 acaccatcag tgttaccggg tggggtgatg gcagcaagta cgatgtcgat tacttgaaag
     1021 ctgcgtttga taacgctttc gccggcggaa atttgacagt agacgtttca tcctatatct
     1081 ataactgagc caccaccgta atcgagtctt tccagctgaa agcgatttta cgctccaaaa
     1141 tccaaataga tgggtgaccc acccatctat ttgttttatt aggagggaat ccagacgctc
     1201 gcgttcaccc tatttttctt gattccgtct ttaatctatc ccgcgtggat atatgacccg
     1261 cgacacgcga agctcttcgt gttttcgctg gtcgtctttg tctgtctgta ccgtctgctg
     1321 aagaaacgtc gtttcgcctt tgaagcgccg cccctccttc tcggaatcct ttgcgccttt
     1381 gcccttttcc tgatcccggc gatccctctc caaaacgaga gggcgcttct gtttttcggg
     1441 atcgtgttct ttattatcat tggcgcctcg gtctttcgct tcgaagactt cagcgtcgcc
     1501 gccgtccaaa agtggactgt cctgtccgcg ctctggattt cgtttatcgg tatccagaac
     1561 ttttacggga tcaccttctt caacatctac gtcccatcgg ctttcgatag gtcctcgatc
     1621 atcgcgaccg tcgggaacgt caattacgtg tccaactacc tcgcctcgat cctgccgatc
     1681 acgatcgccg cgtatttaac gatggaagac gggcgatggc gtttcctcgc cttcatcgcg
     1741 accgtcgcct tctcctccca cgccctggac tgggctatgg cgnnnnnnnn nncgagggat
     1801 cgactccctg gcgtatgggc gtcggccgtc gccgctgccg tcaccgttct gtgggggcag
     1861 acccgctccg tttaccttgg actgttcgtc gccttccttg tcttcttctc tttattgggg
     1921 acttccgatc ggcgccacct gatcaccaaa cgcaaactgg cgggtatggt cctcggggtg
     1981 gttatcatcc tcgcgctcta cgcctttccg cccggcgtgc cggaaaaccg aaggcccctg
     2041 cggctgtcgg tgagtcgggc gcaagagctt caactcccct atgacgaagc cacaggcagc
     2101 ctctaccgtc gtgtcttcga atggaagacc gcgttggaga tgttcaccca ctctcctttg
     2161 tacggttggg gttggggctc ctacatccta ctctcccagg atttccaggt gaaagtgacc
     2221 gaaaaagacc ccgcttattt cgggttctac gaaaaaagcg cggaggcgca cagcgacttc
     2281 ctccagatgc tggccgagac gggaatcatc gggttcgggg tatggatcgc gttactgctg
     2341 tacatcggga tcttaggcgt caagcgctgg ctcgcgacga aaaacctgat gatactggcc
     2401 gccctcagcg gctggttgat gatcctcgtc cacgctttaa cggagttccc gttgcacatg
     2461 atgccctccg ctgggatttt cgcggtgttc agcgggtttt tggtgagcga gggtaaacgg
     2521 aagaccttcc cacgagccgt cggattggcc ttcctgtttt taaccctgtt tttctccttc
     2581 atcgccctga aaacggccct tgcggacagc ttttacgcgt atgggattta tcaaagggag
     2641 aaggcgcaga atcaatactt gaaagatatg gaatccgtcg gccgggcgat cgtgctctct
     2701 tcgaagggaa gcgaagaaac gcccgaatgg ctcgaggatg cgattcgcaa agaaaaagcc
     2761 gccgccgccg aacgattgtc ttcctcttat tatagccaat acctgttttt caccaatgcc
     2821 ctgatcgcgg atcccggtct ttcgagcgcg acttacgaaa ttgctacgtt gatcggcaag
     2881 atggaggaat tggtcccgcg tccgcccttc ctgctgtttg atttcccgcc cttccgatac
     2941 acgggtgtgt ccgccttgcg ggaggcaccg actgaatacc ccgaacttgg gcgttgggtt
     3001 ttcaaattga aagttcaaga acgcgagcga atcgagtacc tttaccgata cttccggggc
     3061 ctttgccttt ctatcaactc gatgatcgac cccgccgttt acctcaatat cggtcgctcg
     3121 gcgaatgaga tgctcgtgct gtacgaagaa tgggatgtcg aagaacccga ggaaagggcg
     3181 ctttggttaa cttggatgct ctacgggtat gagaaggctt tccggctgaa cggcgcccgt
     3241 cagtacaccg aagacctcga gctggaccac ctggacctcg aatacttaga cgcggtgatc
     3301 cgccacggtg tcgacgtcga agagcgcgtt accgaggtac taggcttcag gcgacggctt
     3361 gcccagcaca ctctcaaaaa agattggaga tttcccaaaa aatggtataa ttactttgtc
     3421 gaaaaaatgg atgacggtta ctttgcggga cgcccgacct atcgagaccg atttatcgag
     3481 gtctttgaag agtacgcccg gcgatatcga gaaatggaag ggtatttccg cgaaatggat
     3541 aacgccttac aatcgaaaga gacgaagatt agcgccgcgg atagatacga gctctatcga
     3601 gatatgaaag acatcgaacg gttccttgaa gatttcgagc ggcggttttc aaacggcgct
     3661 gcggagggat aggaatgaga agagctttca cgatagtcga actcttgatc gtcttgggca
     3721 tcatcgcctt actcacttct gtagtcataa aagtatctgc cgatgcggtc atccacgcgc
     3781 gcgcagtgca ggtggcagtt aacctgacgg cgattatgag agcggcgcaa agccggttgt
     3841 tgacgaacag cgcaaccacc gccgatcttg ctgatttgga tatcgatctc gagttccctg
     3901 acgattacgc tctcgatatg gtctatcgag atagtgattc gtccgtcgaa gtcaaggtct
     3961 cttacaccgg catcgtcagc aaaagccata tctccggcct cctcgctcct gacgcgacag
     4021 tgtccgagac cgaaaatgaa atcgcctatt ccaaggtctt tcccgtctat tggtagggct
     4081 cgcagcgcct cgcgcctcaa gcgcccgccc ccattcgggg cacacggtcg ttacggcttc
     4141 aaaaccccga aacgaaattt actcctgaac gaaatcaccc cttttttgca ggggccgaaa
     4201 taaaggtgat ataatagccc tacatataag cgtggaaaat gcttttttta ggaaaataca
     4261 taaggaaagg gggtgtcctg atcctttcta aaaagagagg acagggaatt atgcgttcga
     4321 aaaggttcta ttctttaccc gtatttctcc tcgtgctgct gttggccctc atcggctgtt
     4381 tccccgttgt aaaacccgat aagacgccgc cggtggtgga aactccggtc atcaccttat
     4441 cagccacgac ggttccggat accggcggcg gtatgacggt gacggtcagc gttgcggcgc
     4501 aagatgcgca cagcaacctg aaagacgcgg tgatcacctt aacttggaac accgtcggta
     4561 cggacttgcg agacagggga agcgtgacga aaacggtggc ctatccggcg gctaagaccg
     4621 gaacggcaac cgcagccttc tcggtgaact tgttgggctc agacatcacg ttgccggtcg
     4681 ggatttactc tttcagcgct tcggcggtag tacaagacgt gaagctgaac aaagccgaac
     4741 cgaagtccgc gacggcagct caaaacttga ccgtgcaaaa atcgtccgag cattctccga
     4801 cgatcaacgt gacgtacacg cctacttcag gcgggaaagt gggtatcccg ttcacggcga
     4861 ccgtcgtagg cgcagataca gacggcgacc tcacttattt agagttctac attctgtcgg
     4921 acaccgcgac ggtggtttcc agaatggcga cctccaccgg tacgacgagt ttgacgatta
     4981 cgaccgagca aatcactcta gcgatgctcg ggatggggcc cggagacttc tccaagaccc
     5041 tctattggta cgcgctcgcg cgagatgccc aggggcacgt gacccaatac acgaacaaat
     5101 cgataacggc ccagcggatg gacttggcgg tcatagagtc gatcggtcac gtccgaacgc
     5161 cgagtgagcc gaatatgcca tctcactatc gtgtattggc aggcgccaca ccaccggcta
     5221 tcagcgagct tcaggaagac cagcgcttgc cgatgcagtt cggcgtatcg aagatgcgca
     5281 tcaacctgat ggattctatc gtcttggcag gctacaaagc cagggggtat atcttcacga
     5341 ccaccagcga gatattcgat gcgacaccgg acgtatggcg agcggaaggg gccgtgggtg
     5401 ccgacaagat gctctacctc gacatggtca acttttggcg ccaagtcgat ttagacacat
     5461 ccaaatggtt cgttctggaa atattaaaca acgccggaac ccctatagtg cgttatattt
     5521 ggaacatcaa ggcgctcggt gacaccttcg aaccgatggt ctcgatcatc gattcgggga
     5581 aggactatcg cgaaggcgtg cctttagagt tcacgatctg cgcgaccgat gagtttctcg
     5641 aggcaatcac cgacctgcgc gttcgggtca atatcggtca aaaagggtgg agagacctcg
     5701 aagacgtcgt gagcgacatg aacccggccg tcgatcagaa agagggctgg ctcttcgcgg
     5761 ttcgttacca tgtgaaggtc caagtcttcc cggggctgac caattacgcc agcggtctcg
     5821 tggacgacgg cgtcgactac atcgtcagca acgcgatcgc atccctcaac ggaactggag
     5881 cgcaccagaa cgcgaaatgg ttcgacgtct ttcacggaaa gatccccggt atgcccacaa
     5941 cagcggcggg tcgttccgaa tactggtgga agacggacgg gtacggccat atccttgcgg
     6001 aagacgacga caacggccac tggcccggca accgcacccc ggacgaagag ccctacgatc
     6061 ccacaccgag ccacaatacg gatgaactag accatagggc gctcccaaaa tggtcttacg
     6121 tctatgagtt caccgacctg gacacgacgc gcgtcaacga aatccgcaaa gaggctttcg
     6181 tggaaaacac gaccttcaaa gtgtggatct atccggaagt ggtgcgtgtg aatcgagcgg
     6241 acatcgaagc aggcgtgacc aaacccgaag tcctcgacgt cgtggacgtc tcgatcggac
     6301 ccggaacctg gcgcgaccag cacgtggact accagatgca gatagaaata gccgactggc
     6361 gttggggcgg cgaaaacccg gatataggca aggtgtcgca atcggcgaac ttcacgatcg
     6421 atgaggacga agaaacgccg cgcattcaga tatccggttc ctattcggac gacccgtctc
     6481 aatccttcaa gcatttgttc aaagaccaat ggcaatggta tagccccgtg aaaaactacg
     6541 cggtgccgaa cttttttgtg cacgaactcg aggtcttcac gggcgcgagc atcaccatca
     6601 aagcccagga cgacatcgcc ttacacgacc tcctggtctt ctacgattcc gaaaactata
     6661 ccacctgcgt aagcaaaatc gatagcccga aagccgatta cataggcagg aatgacgcct
     6721 tttcccaccc cttaaccggc ttcgattatg tgtatgacga agggatgctc gcctaccggc
     6781 tgatcaaagg caaagaaacg actggagagg ctactttggt tcccttggtc taccagtcct
     6841 tacctgcgac tccgaaggcc ttcccgatag aggagccgcg tatccttcgg attcttggaa
     6901 aagacttcaa gattaacggc aagatcattt ctccgatctt caccttagat aagaactacg
     6961 aatactacgt gaacttcacc aacgatgacg tggtagacag ttgggcgatc gccgcgcacc
     7021 aaaattcctt cgggagagaa aacgacatca tccgcgcgaa ccccgtcgac aaggaatatg
     7081 agacgtacgc cggcgagtac tccacggtct tcctcggctc gaagctcaac accccgggga
     7141 ccaaggagga tcaaaaagcc gaattcacct tccgcgtccc ggatgtcacc ggggactact
     7201 ggctttggat cgtcgcgcga gaccgcagcg cgcaagacac caagctgttt gactatcctt
     7261 tcgacgaaag acacgactat aaagaagata cttacttggg tgagtacccg agttacgcga
     7321 cgaagaaccg ccaatgcgag atcttcggga cgtggtttga cgacggcagc cccaacttcg
     7381 acaagatgta ttacgacgac tcgatgtcgg aagacgacgc cgatatggtc atcctgctca
     7441 gactgcggat caagtctcac agctgggaca tcgtgcgact cgatatacac gaacccgatt
     7501 tggtgagtta tcc
//
LOCUS       DAOPXN010000106         7490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9854_length_7490_cov_5.491728, whole genome shotgun sequence.
ACCESSION   DAOPXN010000106 DAOPXN010000000
VERSION     DAOPXN010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7490
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9854_length_7490_cov_5.491728"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..910)
                     /locus_tag="PKM49_06755"
     CDS             complement(347..910)
                     /locus_tag="PKM49_06755"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNY82549.1"
                     /translation="MQPLDMKKQFGIFPMPIVVIGTLHGTKVNFMTAAWAVQVDHNPP
                     MVGMALSKDHLTSANIKTAGWFSINIPTADHVVATDYVGIHSGSETDKSNAFPVFYSR
                     QKKAPMIEGCALSMECRLVQTFEINSDPFFIGKIEALFANGNYLTDDKPDFSKMPPLL
                     YCSTDGSYWQVGERVAKAWSVGRKKDD"
     gene            complement(1092..2546)
                     /locus_tag="PKM49_06760"
     CDS             complement(1092..2546)
                     /locus_tag="PKM49_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNY82550.1"
                     /translation="MSDISTKSPHRSRCVLVVEDDEGLQKLIIKRMQKAGYQTLGANT
                     AREALKLVLQWNEENSELVLLLDQNLPDMTGREMLEVLKKQNSRIPFVVMTGQGDERL
                     AVEMMKLGAADYLLKDLNLIDLLPEVFNRLFARLETEQRLKAAETLIRAAAEEHRILL
                     DNMQFQVWYLTDPLTYGAVNETHAAFLGARKEELEYKNKLSLPENYVIEAFWRGNEAL
                     FKGKVVQIEEWVPHFSGEDRCIFIIKKPIIGEKGKVTSAVCSAEDVTERKRAERALLE
                     SQEQIRIQQLKFQEDLLLSIIQILELYDIYTRGHSENVARYSSLIARKINPDPDWVKQ
                     VYWAGLVHDLGKMLVPTQVLNKPGRLTDEEYELVKMHPVWGAKVLMTSEKLLDIGRYV
                     LHHHERYDGQGYPDRISGSEIPLASRIICVADAYDAMTSHRSYRTPLTPQQAKAELVQ
                     NKNLQFDPAIVDIFLSLNSESPEVPEGPGWPRYT"
     gene            complement(2610..3056)
                     /locus_tag="PKM49_06765"
     CDS             complement(2610..3056)
                     /locus_tag="PKM49_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY82551.1"
                     /translation="MKQEVVILEAEDEEGHADLIRRNLSRAGITNKLLLFKDGQEISD
                     FLFRNGDGPHREPGIRYVLLLDIRMPKVSGTEILRKIKADPELRKMPVIMITTSDDPY
                     EIELCHKLGCNNFITKPVEYEAFANAIRQLGQFLSVVQIPKVNGVV"
     gene            complement(3144..5477)
                     /locus_tag="PKM49_06770"
     CDS             complement(3144..5477)
                     /locus_tag="PKM49_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY82552.1"
                     /translation="MRETPNNRANKLSATEIGFSYHTGVRDARGLMTDLEFIDVDPSF
                     ARLVGVERDRIVGCRARAILPQPFQAAPDWLTAFANEESDRRFRSSWIEVPGEGGTFL
                     ILTCRGAPDRTDHFFSFLFKAADEKSLLYTLEKREERWRYALEGGEVGVWDVNDETGE
                     AYYSPIWKSMLGYEDHEIGNRNEDWESRIHPEDRESVFLEIREERAQRSPISKHEHRL
                     RCKDGSYKWILSLGKSIERAPDGRSLRSVGVHIDIGERKRIEESLKQREQELRVILET
                     TKDGFYTTDKNGQFLSANEAFLHMVGYTLKELLALRFADLSAFQTEEEINARNQRIET
                     NGFEVFETRLRLKNKKSAFFEISASFLTHSQKIICFCRDTTERHTMEEQLRLAKQQAE
                     EANQAKTRFLSTMSHELRNPLNSILGFTELLFDSSLTQEQRKFTQYISSSSRLLLDII
                     GEVLDISKIEAGKMDLAPLPNDLRKVCAEIVQLNLLRAKEKGIELRMKFDERLPESMV
                     FDALRLRQVLMNLIGNAVKFTSVGGVSLRVALKDPKRQDGKQFVQFEIEDSGIGMTEE
                     ARRRVFSEYEQADSTISRQYGGTGLGLSISKRILNLMEASLQVKSIPGVGSVFSFAIP
                     LIIPEDRPKKEADAKASKKPTFESDLKKKIHVLLVEDEPVNLFLAKKLLEKVSPDIGI
                     DQATNGEEALENFLRAQPDIIFMDIHLPILDGYQTTAKIRHFEETIGGRVPIIGVTAI
                     TGKQQIEECYSSGMDDVLLKPLSAVAFEEVLRKWIHP"
     gene            complement(5496..7400)
                     /locus_tag="PKM49_06775"
     CDS             complement(5496..7400)
                     /locus_tag="PKM49_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82553.1"
                     /translation="MQVFKGFTAFVGFVVTYTHRMSFLYINESVSKVMGVTAQAVMED
                     ANVLLDKMDPEDRMMNLRKARESAGALKPYNFVIRTTGSGKPKWIETHAAPTRLPDGS
                     ILWDGFFLDVTERKNAEDRLQESNERLKAVMDSIDSFVYIADMDTYELLFMNEPLIRA
                     WGEIEKGALCWKTIQKDQDGPCPFCTNHLLVDRDGKPTGVYQWEFQNTVTGRWFECRD
                     RAIRWIDGRTVRMEIATDFTNRKLAEEALRESENKFSTLFSAMNEMVVLQELVRDENN
                     KPVDYRILDCNDTFTKITGVEKKMAVGKLGSVVYQSDPPPYLEIFAEVASTGKPRRHE
                     TYYAPFNRYFSISCVSTGIDRFATVTTDVTDSRKAEKIIEEKNKELEQIIYVASHDLR
                     SPLVNVDGFSRELEYVIDDFKKLLNTEESNSVSFETVFRSALPDLEDALRHIRASALQ
                     MDALLKGLLRLSRSGRAAMTIVPLEMDLVVQNVLSAMDYQITTAGAQIDVEPLPNCYG
                     DEVLLTQVMTNLIGNAIKYLDPKRPGKIRISGTVHLRNSIYCVEDNGIGIASEHQEQI
                     FQLFHRLNPAKTDGEGLGLTIVRQIVSRHDGRVWVQSELGKGSKFCFEIPTKRQPSPQ
                     KESPSPKTLT"
BASE COUNT         1597 a         1964 c         1899 g         2030 t
ORIGIN      
        1 ctggttaccc cgcgataaca cacacggtga tcgcggcgta agcgcttctt cttttcgcga
       61 cggcgctacc ctttcgaaaa agacaaaagg cggatcgcga gtttcgtgag ggatcgcgag
      121 acttcgtggg cttccgaagt cagcccgctg gttacctggt taccccgcga taacacacac
      181 ggtgatcgcg gcgtaagcgc ttcttctttt cgcgagagcg cgataccctt tcgagaaaga
      241 caaaaggcgg atcgcgagtt tcgtgaggga tcgcgagact tcgtaggctt ccgaagtcag
      301 cccgctggtt accccgcgat aacctataaa tcgcggcgct cccaggtcag tcgtcttttt
      361 tgcgtcccac gctccaagcc ttggccactc gttcgcccac ttgccagtag gacccgtcgg
      421 tcgaacagta caataacggt ggcattttcg agaagtcggg tttgtcatcg gttaagtagt
      481 tgccgttggc aaacaacgcc tcgattttcc cgatgaaaaa cgggtcagag ttgatttcga
      541 aggtttggac cagccgacat tccatactca gcgcgcagcc ttcgatcatc ggcgctttct
      601 tttgtcggct ataaaaaacg gggaatgcgt tcgatttgtc ggtttcgctg ccggagtgga
      661 tgccgacgta gtcggtggcg accacgtgat cggctgtcgg gatgttgatg ctgaaccacc
      721 ccgcggtttt tatattggcg gaggtcaggt gatctttgct caacgccatt cccaccatcg
      781 ggggattgtg gtcgacctgt acggcccacg cggcggtcat aaagttcacc ttggttccgt
      841 gcagcgtgcc aatcacgacg atcggcatcg ggaaaatacc gaattgcttc ttcatatcca
      901 gcggttgcat aagacaatcc tcctttaatt tccctgtttc tatggatatt cgaccgtcta
      961 cgccgtagcg tgaaacgagc cttgtgtttt ttaatctatc gaagaaatcg cgataaaaga
     1021 acctctctcc cacaagaaag acgtctacga gagaggccgc gagttatata aaagaaacca
     1081 aatgcggttc cttaggtata tcttggccat cccggtcctt ccggaacctc aggtgattcg
     1141 gaattgagag acaaaaagat atcgacgatt gccggatcga actggaggtt tttgttttgt
     1201 accagttctg cttttgcctg ctgcggtgtg aggggcgttc gataggagcg gtggctggtc
     1261 attgcgtcgt aggcatcggc gacgcagatg attctggaag ccaggggtat ctcgctgcca
     1321 ctgatccggt ctggatatcc ttggccatcg tagcgctcgt ggtggtggag aacgtatcga
     1381 ccgatatcca acagcttctc cgaagtcatc agcaccttgg cgccccaaac ggggtgcatc
     1441 ttcacgagtt cgtattcttc atccgtgagc cgaccgggtt tgttcaggac ctgcgtgggc
     1501 accaacatct tcccgagatc gtgaaccaat ccggcccaat acacctgttt tacccagtcg
     1561 gggtcggggt tgatctttcg cgcgatcaac gaggagtacc gcgccacgtt ttccgaatgg
     1621 ccccgggtat atatatcgta cagttccaat atctggataa tagataaaag caggtcttct
     1681 tggaatttca gctgctggat ccgtatttgt tcttggctct ctagcaaagc cctttctgcc
     1741 cgttttcttt cggtgacgtc ctcggcggag cagacggcgc tcgtcacttt ccctttttct
     1801 cctatgatcg gtttcttgat gatgaaaatg cagcgatctt ctcccgaaaa atgaggcacc
     1861 cactcctcga tttgcacgac ttttcctttg aaaagcgctt cgtttcctct ccaaaaggct
     1921 tcgatgacgt agttttcggg caatgagagc ttattcttat attctaactc ctctttcctc
     1981 gccccaagaa acgcggcgtg ggtttcgttg accgcgccat atgtcaaggg atcggtgaga
     2041 taccatacct ggaattgcat attgtccaga agaatccggt gttcttccgc tgccgccctg
     2101 atgagggttt cagccgcttt cagccgctgc tctgtctcca gtctggcgaa caaccggttg
     2161 aacacttcgg ggagtagatc gatgaggttg aggtccttca gtagatagtc ggctgccccg
     2221 agtttcatca tctcgacggc gagccgttcg tcaccttgcc ccgtcataac cacgaacgga
     2281 atacgggagt tttgcttttt taggacttct aacatctcac gcccggtcat atcgggaagg
     2341 ttctgatcca agagcaggac cagctcggaa ttctcctcgt tccattgaag aaccagttta
     2401 agcgcttctc gtgcggtgtt cgctcctaag gtctggtatc ccgctttctg cattctttta
     2461 ataatcagtt tttgcaaacc ttcatcgtct tccacgacca atacgcaacg ggatctatga
     2521 ggcgatttgg tggaaatatc actcacaacg cttcctcctt cgaatgtgaa gcgaatctat
     2581 gctgtcgtaa agcgcagggg tgacgagtgc taaacgaccc cgttgacttt aggaatttgg
     2641 acgacagaca agaactgacc taactgccga atggcattgg caaaggcttc gtattccacc
     2701 ggtttcgtga tgaagttgtt gcatcccaac ttatggcaca gctctatttc gtatggatca
     2761 tcagaggtgg tgatcataat tactggcatt ttcctcaatt ccggatctgc ttttattttt
     2821 ctgaggatct cggtcccgct caccttcggc atgcggatgt ccaacagtag gacgtagcga
     2881 atgcctggtt cacggtgcgg tccgtctcca tttctgaata ggaaatcaga gatctcctgc
     2941 ccgtccttga aaagcagcag tttgtttgta atccccgccc gactcaggtt cctgcggatc
     3001 aaatcggcgt gaccttcctc gtcttctgct tccaagataa cgacttcttg tttcatagct
     3061 tctcctcctg tgtctattct accatcttcg cgctctgaaa ccgtcggcct tttttttcgg
     3121 tttgggcctc gtcgtgcggc tgtttacggg tgtatccact tgcggaggac ttcttcgaaa
     3181 gccaccgctg aaagaggttt aaggagaaca tcgtccattc cggaagagta gcactcctca
     3241 atttgttgtt ttccggtaat cgctgtgaca ccgataatgg ggacacgtcc gcctatcgtt
     3301 tcctcaaaat gcctgatctt cgccgttgtc tggtaaccat ccaatatcgg cagatggata
     3361 tccataaaaa tgatgtccgg ttgcgccctt aggaagtttt ccaatgcttc ctctccgttg
     3421 gtcgcctgat cgatgccgat gtcggggctc actttctcga gcagcttttt ggctagaaag
     3481 agattcacgg gttcgtcttc cacgagcaac acgtgtattt tcttcttgag atcgctctcg
     3541 aatgtgggtt ttttagaagc cttcgcatcc gcttcttttt tcggtctgtc ttccgggatg
     3601 attagcggta tggcgaaaga aaagacggac cccacaccgg gtatactctt cacctgtaga
     3661 gaggcttcca tcaagttcag gatcctttta gaaatagaga gacccaatcc tgtgcctcca
     3721 tactgccgac taatcgtcga gtcggcttgc tcgtactcag aaaacactcg tcgacgggct
     3781 tcttccgtca tcccaatccc tgagtcttcg atttcgaatt gtacgaactg ttttccatcc
     3841 tgtcgtttcg gatccttcag ggcgacacgc aaagaaacgc ctcccaccga ggtgaatttt
     3901 accgcgtttc ctatgaggtt catcaacacc tgtcggagcc ggagcgcatc aaaaaccata
     3961 ctctcaggaa gtcgttcgtc gaatttcata cggagttcga tacccttttc ttttgctcga
     4021 aggaggttga gttgcacgat ttcggcgcac acttttctca ggtcgttcgg gagcggcgcc
     4081 agatccatct tgcccgcttc tatcttagag atgtcgagaa cctcgcctat gatatcgagt
     4141 agaagccgtg aggagctgga gatgtactgc gtgaactttc gctgctcttg ggtgagcgag
     4201 gaatcgaata ggagttcggt aaaccccaaa atactgttca aggggtttcg cagttcgtgg
     4261 gacatcgtgg aaagaaagcg ggtcttggcc tggttcgcct cttccgcttg ttgtttcgcc
     4321 aagcgcagct gttcttccat cgtgtgcctt tcggtggtgt ccctacagaa gcagatgatc
     4381 ttttgcgaat gggtcaagaa agaggccgaa atctcgaaaa aagccgactt cttgtttttc
     4441 agacggagac gagtttcgaa gacttcgaac ccattggtct ctatccgctg attcctggca
     4501 ttgatctctt cttccgtttg aaaggcgctt aggtctgcga agcgcaacgc gagcagctct
     4561 tttaatgtgt accccaccat atgtaaaaaa gcttcgttag cgctgaggaa ttgtccgttc
     4621 ttgtccgtgg tgtagaagcc gtctttcgtc gtctccaaaa tcacacgcag ctcttgttcc
     4681 ctttgcttga gcgattcttc tatccgtttc ctttcgccga tatcgatgtg gactccgacg
     4741 gacctcaggg accggccatc gggcgcgcgc tcgatcgatt ttcccaaaga gaggatccac
     4801 ttataggatc cgtctttgca ccgcaaacgg tgttcgtgtt tggatatcgg cgacctctgg
     4861 gctcgttcct cgcgaatctc gaggaaaacg gactcacggt cttccggatg gattcgtgat
     4921 tcccagtctt cgttccggtt tcctatttcg tggtcttcgt aacccagcat actcttccaa
     4981 atgggtgagt agtaggcttc ccccgtttcg tcattgacat cccagacccc cacctcgcct
     5041 ccctccaggg catagcgcca ccgttcttcg cgtttttcca gggtgtaaag aaggcttttt
     5101 tcgtctgcgg ctttaaacag gaaggaaaag aaatggtccg ttcggtcggg agcccctctg
     5161 caggtaagga tcaagaacgt gcctccctcg cccgggacct ctatccaaga ggaacgaaag
     5221 cgtcgatcgg attcttcatt ggcgaacgcg gtcagccaat cgggcgcggc ctgaaagggt
     5281 tgcgggagta ttgccctagc gcgacaaccg acgatccgat cgcgctcgac gcctacgagg
     5341 cgtgcaaaag aggggtccac gtcgatgaac tctaggtccg tcataaggcc gcgagcgtcc
     5401 cggacgcccg tatgataaga aaagccgatt tcggtggcgc tcaacttatt cgcccgatta
     5461 ttaggcgttt ctctcatagt agaacagttc cctccttagg taagggtttt tggcgacggg
     5521 ctctcttttt gtggagacgg ttgcctcttc gtcggtatct cgaagcagaa cttgctaccc
     5581 tttccgagct cggattggac ccagaccctt ccgtcgtgac ggctgacgat ctgccttacg
     5641 atggtcagtc ccaggccctc tccatcggtt ttggccggat tcaaccggtg gaagagctgg
     5701 aaaatctgct cctgatgttc ggaggcgata ccgatcccat tgtcttccac acagtagatg
     5761 ctgtttctca agtggaccgt gccggatatc cggattttcc ccggtctttt agggtcgagg
     5821 tacttgatgg cgtttccgat caagtttgtc atcacctgtg tgaggagaac ctcgtctccg
     5881 taacaattcg gtagcggctc tacgtctatc tgtgctccgg cggtcgtgat ctggtagtcc
     5941 attgcagaga ggacattttg aacgacgaga tccatctcca atgggacgat cgtcattgcg
     6001 gctcttccgg aacgagatag gcggagtaac cctttgagga gggcatccat ctgaagggcg
     6061 ctcgcgcgga tgtgtcgaag cgcgtcctcc aagtcgggta aagcggaacg gaaaacagtt
     6121 tcaaaagaaa cggagtttga ctcttcagtg ttcaggagtt ttttaaaatc gtcgatcacg
     6181 tattccaatt cgcggctgaa cccatcgaca ttcaccaagg gggaacgcaa gtcatgagag
     6241 gcgacgtaga tgatctgctc cagttctttg ttcttttctt ctataatttt ctccgctttc
     6301 cgtgaatcgg tcacatcggt cgtgacagta gcgaatctat ctattccggt ggagacacaa
     6361 gaaatggaaa agtatctgtt gaatggggca taataggttt cgtggcggcg cggctttccg
     6421 gttgacgcga cttccgcgaa tatttcgaga taggggggcg gatcagattg gtaaaccacg
     6481 gaaccgagct taccgacggc cattttcttt tctacccccg tgatcttggt gaaggtgtcg
     6541 ttgcagtcca gaatgcggta gtctaccggt ttattgtttt cgtctcgtac gagttcttga
     6601 aggacgacca tctcgttcat cgcggaaaac agcgtgctga acttgttttc gctctcgcgt
     6661 agggcctctt ccgctaattt acgatttgtg aagtcggtgg cgatctccat acggacggtg
     6721 cgtccgtcaa tccatcgaat ggcgcgatcg cggcattcga accatctacc ggtgacggtg
     6781 ttttgaaact cccattgata taccccagtc ggtttcccgt ccctgtcgac cagcaggtga
     6841 ttggtgcaga aggggcaagg accatcctga tccttctgta tggttttcca acatagcgct
     6901 cctttttcta tttctcccca agccctgatg agcggttcat tcataaaaag cagctcgtaa
     6961 gtgtccatat cggcgatgta gacgaaggag tcgatgctgt ccatcaccgc tttcaaacgt
     7021 tcgttcgact cctgcaaccg gtcttcggcg ttttttcgtt cggtgacatc caagaagaag
     7081 ccgtcccaca agatactccc atccggtaga cgagtgggag cagcatgggt ttcgatccac
     7141 tttggtttgc cggacccagt cgtgcggata acaaaattgt atggtttcag agcccctgct
     7201 gactcccgag ccttccggag gttcatcatt ctgtcttccg gatccatctt atccagcaaa
     7261 acgttcgcgt cttccatcac tgcttgcgcg gtcacgccca ttaccttcga aacggattcg
     7321 ttaatgtata gaaaactcat cctgtgcgtg tatgtaacaa caaacccaac aaacgctgtg
     7381 aagcctttga atacctgcac tcagaaaaaa aggattccgt tgttcttaca gaaggccgag
     7441 agaaataggt tacagcgaaa atcgaaactg cctttctaat gaaataaaat
//
LOCUS       DAOPXN010000107         7465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9892_length_7465_cov_4.894872, whole genome shotgun sequence.
ACCESSION   DAOPXN010000107 DAOPXN010000000
VERSION     DAOPXN010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9892_length_7465_cov_4.894872"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="PKM49_06780"
     CDS             complement(<1..330)
                     /locus_tag="PKM49_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82554.1"
                     /translation="MYLSLFDYNVFSQRFIGFENFATLFSDWLFLKALGNTFLYTFGV
                     VPLWLGKALIVTVLLHPFRNSIKTFYKALFYLPHVTSSVIVSMIWLWVYNPNYGLLNY
                     FVKSVGLA"
     gene            complement(505..1851)
                     /locus_tag="PKM49_06785"
     CDS             complement(505..1851)
                     /locus_tag="PKM49_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNY82555.1"
                     /translation="MKHRKILLVVLLLTLLSLSAFSAKITITWWINPWRIAPPGFPAD
                     KAPSSEDYPKWASEEFMRLHPDVEVNYVVVGNQEYSQKMAAAIATRTQPDFFKGPVWD
                     SRWAQSGLLEPIDAYLSQEDREDFYQVVLDEGLVNGKRYIWPWNFGTNGMGTAMLLYT
                     PDFDKAEIDWRHIVRNGWTMEEFVEACKKLTWDSNNDGEIDHYAISFGAKDIHNIINF
                     LYAFGGRLSDEAETTVTLNTPEAVAGLQFVIDLVNKYKVAPKGVEALGVYDVIGDFHS
                     HRTSIGFGGPYEIGRITRYILAGTLKEMFYPVLAPFPHVEGKDPVAHTSGSGFIIFKQ
                     NDQAKRDMVFEFVKFLTNKQNLALLETLQYLTARDSVNQRLYLNDPYMNKQVKTYGEI
                     MDKYGMSFFGSQEFPWSQIEKYIISAFEAAFAGSKTPKKALDDFVAEADKIVKKAK"
     gene            complement(1981..2574)
                     /locus_tag="PKM49_06790"
     CDS             complement(1981..2574)
                     /locus_tag="PKM49_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNY82556.1"
                     /translation="MDHSQIKLIIFDVGGVVYGRTNVWRKAAQELGVSYRKFNEYATL
                     SGIQGFQRGLISQEYFMKVFQHLTHHRTPEDLWTRFFDPAPIPETIDVIERLKKRFRV
                     VAGTNTVTPHYEVHTQREDYAIFPKVYASHLIHLAKPDPQFYTHILQAEAVSPVESVF
                     IDDSIQNVESALKLGIHAIHFTEGKALQEALSPLLVY"
     gene            complement(2714..3973)
                     /locus_tag="PKM49_06795"
     CDS             complement(2714..3973)
                     /locus_tag="PKM49_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNY82557.1"
                     /translation="MRADNRLKETALEIVRCAIRENLPDRALSRALAKTTLPGPLTLI
                     AIGKAAWRMARTALECLGPRIATGIVLTKYGHSEGPLRGIEIFEGGHPLTDENSILAT
                     RAILERVGATSVSQRILFLLSGGGSSLFELPIEGLHLEDLKAINERLLNSGASIHEIN
                     AVRKRLSGVKGGRFARYAHPRPISAFILSDTLGDRIDNVASGPVTVDRTTTREALDII
                     RRYSIHLPASLSKAMERPSPNALPGVETSVIGDVRSLCRSAQEKAREEGYAPLLLTTQ
                     FDGEASACAKILTLVGKEIVHSQTPISAPAAVFIGGEALVRVLGKGKGGRCQEMALLA
                     AKEIAGYESLCFFACGSDGTDGPTDAAGGIATGDTVLRLAEKGLSPDDFLRNNDSHEA
                     LKAADGLIYTGPTGTNVNDLYGILVGN"
     gene            complement(4037..5662)
                     /locus_tag="PKM49_06800"
     CDS             complement(4037..5662)
                     /locus_tag="PKM49_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82558.1"
                     /translation="MAFKTMIPLDFRLLLRYSLKNKKRRNKEKVRSGPLASAFSSWFG
                     YLLAPLIFVVFLTPVYYDLFLKLAVPLVKFGMNSPFLVSDVLLAPVFTVTGLLFSLQF
                     AALLVVELFENDMTPLLLTMPIRRSSIFIVSSMNTLLMTNFGISMTVSISLAYSMLNR
                     DRLWWGILGICLWMLFLIALGVLFGLLLAKLIGKTAAKRASQLITFLIVILVVISPSV
                     VTPVNGLNPGSVLRSLSGSANLFLSSGWPHYWLMVALKGQLAFLILLLAVTVSLFTFV
                     FVCANTLNYSPARKNRDRRRVEVVGEKSGAKNRGSFPLLKKELRLMFRESGIIWYLIY
                     PLAFPILLVIGRYHQTPFVSLLFMIVATMYSGMVSVTMMVFEKRIWPVPTYFPVSFLS
                     SIRLKVFIPVVLFVLESLILLGLFIAQTPSAIYDVFMIAPIAMILYYCSLLGIRLFLQ
                     DPDRDISQRNRLLKGSEVLRLEAVSIGFSVGFYGAFYCLKTFLIEGSFLFFERLPPWS
                     VILLLAGILIALLALLLRSIQMERRKIHRLLVE"
     gene            complement(5668..6402)
                     /locus_tag="PKM49_06805"
     CDS             complement(5668..6402)
                     /locus_tag="PKM49_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82559.1"
                     /translation="MIIAKALTKKFGNLTAVDNLYLHVRSGEIYSFLGPNGAGKTTTI
                     RMLTGSLSPTSGAIEIDRKRYEQDEITIKRGIGVVPDEPRMYENLKGIEFIEFVKGIY
                     HLDPKLTDQRFNELCAAFDIDFLQTYIGDYSHGMKQKLMVISVLMRRPKVLFLDEPTV
                     GLDARAARVLKTLLDHFRNEGSAILLTTHILEIAEKMSDRIGIIRNGKMVVEGSMEDL
                     RKIGNNQANSLEDIFLDLTSKDASAG"
     gene            complement(6434..>7465)
                     /locus_tag="PKM49_06810"
     CDS             complement(6434..>7465)
                     /locus_tag="PKM49_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNY82560.1"
                     /translation="KAEATRMVRMAIDQGVNYIDTAYGYHKGQSEHFTREALGDGYRD
                     KVFLATKMPTYLVKEKADFERFFQEQKTKLGTDRIDMYLLHTLNQKRWDLINGLDVLE
                     FLEEKRKAGEIRFFGFSFHDDYPAFESIVNTYDWDFCQIQFNYMDTEVQAGLRGLRLA
                     HAKGMALIVMEPIKGGKLANPSPEIRRILSEAQPTWSPAEWALRWVANFPEVSLILSG
                     MSTMAQAQENVKVANQMKANTFAQKENEAIKKAVEAFQSYRQIPCTECGYCQPCPEGV
                     AIPDIFSLYNEGKMFGVMETAITQYRTLFSSKHKADRCVACGKCETLCPQGLPIIDLL
                     KQCHEALNS"
BASE COUNT         1812 a         1926 c         1928 g         1799 t
ORIGIN      
        1 ggcgagtccg acagatttga cgaagtagtt gagcaacccg taattcgggt tgtacaccca
       61 aagccagatc atagagacga tgaccgagga ggtcacgtgg ggcagataga acaaggcctt
      121 atagaaagtt ttgatggagt tgcggaacgg gtggagcaag acggtgacga tgagcgcttt
      181 ccccaaccag agcgggacga caccgaaggt gtagagaaag gtgtttccca acgcttttag
      241 gaatagccaa tcagaaaaaa gcgtggcgaa gttttcgaag ccgatgaacc tttgggagaa
      301 aacgttgtag tcgaagaggc tcagatacac gccccaacct actggaatta gaaagaacaa
      361 tgtgaacatg ccaaaatgag gaaggacaaa aaagtaacct gcccgtcctt tcacgacgcg
      421 tttcgccagt gtcatccgat caccaacctg atcacaaaca gtaggggcgc gccttcacgc
      481 gccccgtgat taagataggc tcggttactt agcctttttg acgattttat cggcttcggc
      541 cacgaagtcg tccagagctt tcttcggtgt ttttgatccc gcgaaggccg cttcgaacgc
      601 gctgatgatg tacttttcta tctgtgacca ggggaattct tgcgaaccga agaaggacat
      661 cccgtacttg tccatgatct ccccgtaggt cttcacttgt ttgttcatat aggggtcatt
      721 caagtacagt ctttggttga cggaatcgcg tgcggtgagg tattgcagcg tttcgagcaa
      781 ggcgagattt tgcttgttcg tcaagaattt gacgaactcg aagaccatat cgcgtttcgc
      841 ttgatcgttc tgcttgaaaa tgatgaaccc gctcccgctg gtatgcgcga ccggatcctt
      901 tccttccacg tgcgggaagg gcgcgagaac cggatagaac atctctttta gcgttccggc
      961 gaggatgtac cgcgtgatcc gcccgatctc gtaaggtccg ccaaagccga tgctcgtgcg
     1021 gtgagagtgg aaatctccga taacgtcgta cacgccgagg gcctcgaccc cttttggcgc
     1081 gactttgtac ttgttcacca ggtctatgac gaactgcaaa cccgcgacgg cttcgggagt
     1141 attcaatgtg accgtcgttt ccgcttcgtc gctcaaacgg ccgccaaagg catacaagaa
     1201 gttgatgatg ttatgaatgt ctttcgcgcc gaaactgatc gcgtaatgat cgatctctcc
     1261 gtcgttgttc gaatcccagg ttagcttttt acaagcctct acaaactcct ccatcgtcca
     1321 accgttccga acgatgtgtc tccaatcgat ctccgctttg tcgaaatcgg gggtgtagag
     1381 gagcatcgca gttcccatac catttgtgcc gaagttccaa ggccaaatat aacgtttgcc
     1441 attgaccaac ccttcgtcca acacaacttg gtagaaatct tcccggtctt cttgcgacaa
     1501 ataggcatcg atgggctcca gcaatccgct ctgggcccag cggctatccc aaacgggccc
     1561 tttgaagaaa tccggctggg ttctcgtcgc gatggcggcg gccatctttt gcgaatactc
     1621 ctgattgccc accaccacgt aattcacttc gacgtcgggg tgcagcctca taaactcctc
     1681 ggaggcccat ttcgggtagt cttcgctgga aggcgcctta tcggctggga accccggggg
     1741 cgcgattctc caaggattga tccaccaagt gatggtgatc tttgccgaaa acgcggacaa
     1801 actcaacaag gttagcagca gaacgacgag cagaatcttt cggtgtttca taccactcac
     1861 tcctttctgt ttgaatgtgt tcctgctatc tttcgacgat gcgcccgaat cctcgataga
     1921 aacgagcggc tcgttttgat agaccacttt ttgtcgaaaa aataaaccgt ttgggtagga
     1981 ctagtagacc aataaaggcg acagcgcttc ctgcaaagcc tttccctcag tgaagtgtat
     2041 cgcgtggatt ccgagtttta gggccgattc tacgttctga atggaatcgt cgatgaacac
     2101 ggattcgacg ggcgaaacgg cctccgcctg caggatatgc gtgtagaact gcggatcggg
     2161 tttggccaag tgaatcagat gcgacgcgta aactttcggg aagatggcgt agtcctctct
     2221 ttgcgtatga acctcatagt gcggagtcac ggtgtttgtg ccggcgacga ctcggaatct
     2281 cttttttagt ctttcgatca cgtcaatcgt ctcaggaatc ggcgcgggat cgaaaaatcg
     2341 ggtccaaagg tcctcgggcg tgcggtggtg cgtcaggtgt tgaaacacct tcataaagta
     2401 ctcttgactg atcagtccgc gctgaaagcc ttgaatgccg cttaaggtcg cgtattcgtt
     2461 gaatttccta taggaaaccc ccagctcttg cgccgctttc ctccagacgt tcgttcggcc
     2521 gtacacgacg cctccgacat cgaagatgat gagtttgatc tgagaatggt ccaccttgtc
     2581 cactcctttt ttaaagctgg atcgcgaatt atcccgaagg gatctttaag cgatcgtagt
     2641 tggaacgatc aacaactgtt ttattatacc cgatttctta ttagaaataa cctaaaatcc
     2701 ccgtcccgag gtattaattc cccactagaa ttccgtacag gtcgttgacg ttggttcccg
     2761 tcgggccggt atagatcaac ccgtccgccg ctttcaacgc ttcgtgtgaa tcgttgttcc
     2821 gtaaaaagtc atccgggctc agcccctttt ctgcgagtcg gaggaccgta tccccggtcg
     2881 ctattccgcc cgcggcgtcc gtcgggccgt ccgttccgtc tgatccgcag gcaaaaaaac
     2941 agagggactc ataacccgcg atctctttag ccgctaacaa agccatctct tggcaacgtc
     3001 ctcccttacc tttcccgagg acccgcacta gggcttcccc tccgatgaac accgcggcgg
     3061 gtgcggaaat cggcgtttga gagtggacga tctcttttcc taccaacgtc agaattttgg
     3121 cgcacgcgct ggcctcgccg tcaaattgcg tggtgagcaa aagcggcgca tatccctctt
     3181 cccgggcttt ttcttgcgca gagcgacaga gggagcgaac gtcgccgatg acggaagtct
     3241 ccacaccagg caaggcgttg ggagaggggc gttccatcgc tttcgataga gaagcgggaa
     3301 ggtgtataga ataccttcgt atgatatcga gggcttcccg ggtcgtggtt cgatcgacag
     3361 ttacgggtcc agaggccacg ttgtctatgc gatctcccaa agtgtcggaa aggatgaagg
     3421 ccgagatggg ccggggatgt gcgtaacgcg cgaaccgccc tcccttcact ccggaaaggc
     3481 gttttctcac ggcgttgatt tcgtgaatag aagctccgct gttcagtaat cgttcgttga
     3541 tcgctttcaa gtcttctaaa tgcaggcctt cgatcgggag ttcgaagagg gacgaccccc
     3601 cgccggagag caagaaaagg atacgctgcg acacggacgt agcgcctacc ctttccaaga
     3661 tcgcccgagt cgctaaaatg gagttctcgt cggtcagggg atgaccacct tcgaagattt
     3721 cgataccccg gagcggccct tccgaatgcc cgtatttggt caacacgatc cctgtggcga
     3781 tcctgggccc caggcattca agggcggttc tagccatacg ccaagcggct tttcctatcg
     3841 cgatcagtgt taacgggccc ggtagcgtgg tttttgccag cgctctgctt aaggctcgat
     3901 ccggtaggtt ctcccggatc gcgcaacgga cgatttccag agcagtttct ttaagccgat
     3961 tgtcagcgcg catgtcatac ctccgtaccc agaaggttcg aataaatggt tctatagaac
     4021 gaagtcgagg tgccgctcac tcgacgagca atcgatggat cttccggcgc tccatttgaa
     4081 tcgatcggag cagaagcgcg agtaacgcga tgaggattcc ggccagcagg aggatgacgc
     4141 tccaaggcgg gaggcgctcg aaaaacaaaa aagatccttc gatcaagaac gttttcagac
     4201 aatagaatgc gccataaaag cccacggaaa acccaatcga gaccgcctcc aatctcaaca
     4261 cttcggaccc cttcaacaac cgattgcgct gagagatatc gcggtcgggg tcttgcagaa
     4321 aaaggcgaat gcccaaaagg ctgcagtaat agaggatcat ggcgatagga gcgatcataa
     4381 atacatcata gatcgccgac ggagtttgag cgataaatag gccgagtaga atcagggact
     4441 ccaaaacgaa gaggacgacg ggaatgaaaa ccttgagtct gatcgaagac agaaaggaga
     4501 ccggaaaata cgtgggaacc ggccatatcc tcttttcgaa aaccatcatc gtaacagaca
     4561 ccattccgga gtacatcgtc gcgacgatca taaagagaag agaaacaaaa ggcgtttgat
     4621 ggtatcttcc gattacaagc aagatcggga aagctagggg gtagattaaa taccaaatga
     4681 tcccgctctc tcgaaacatc aacctcagtt ctttttttag aaggggaaag gatcctctat
     4741 ttttcgcgcc ggatttttcg ccaacgacct ctaccctcct tctatcccgg tttttccgtg
     4801 cgggagaata gttcagagta ttggcgcaaa cgaaaacgaa ggtgaacagg cttaccgtga
     4861 cggctagtag caggattaaa aacgccagtt gtccttttag ggcgaccatt aaccaatagt
     4921 ggggccaccc cgacgacagg aagaggttgg cgctgccgct cagggacctt aaaacggagc
     4981 cgggattaag tccattcacg ggcgtcacga ccgaggggct tatcaccacc aaaataacga
     5041 tcagaaaagt gatcaactga gaagcccttt ttgcagcggt tttaccgatg agcttggcga
     5101 gaaggaggcc gaagagaaca cccaacgcta tcaggaagag catccaaagg cagatgccta
     5161 gtatgcccca ccaaagccga tcccggttga gcattgaata ggctaaagag atcgatacgg
     5221 tcatcgagat accaaaattc gtcatcaaca gcgtgttcat agaggagacg atgaagatcg
     5281 aagatcgcct gatgggcatc gtcagcaaca agggagtcat atcgttttca aatagctcga
     5341 ccaccaacag cgcggcgaat tgaagggaga agagcagtcc cgtcacggta aataccggcg
     5401 ccagaaggac atcggagacg agaaacggcg agttcatacc aaatttaacc aacgggacgg
     5461 ccaatttcaa aaagaggtcg tagtaaaccg gtgttagaaa gacgacaaag atcagcgggg
     5521 ccaggagata accgaaccaa gaagaaaagg cggatgccaa aggcccggac ctcaccttct
     5581 ccttgtttct ccgcttttta ttttttaaag aatatctgag taaaaggcgg aagtctaggg
     5641 gaatcatcgt tttaaacgcc atcggtgtca tcctgccgag gcgtctttgc ttgtcaggtc
     5701 taaaaagatg tcttccaaag agttggcttg gttgtttccg attttcctaa gatcttccat
     5761 cgacccttcc acgaccattt tcccattcct tatgatgccg attcgatcac tcatcttttc
     5821 cgcgatctcg aggatgtgag tcgtcaggag tatcgcgctt ccctcatttc ggaaatgatc
     5881 cagcagcgtt ttcaacaccc tggcggcacg ggcatccagt cctaccgtcg gctcatcgag
     5941 gaataagacc ttcggccgtc gcattaagac cgaaatgacc atcaatttct gtttcatccc
     6001 gtgtgaatag tcgccgatat aggtctgcaa gaaatcgata tcgaacgcgg cgcacagctc
     6061 gttgaaacgt tggtccgtaa gctttgggtc aaggtggtaa atgcctttga cgaactcgat
     6121 aaactcgatt cctttaaggt tttcgtacat ccgtggctcg tcgggaacga caccgatccc
     6181 tcgttttatt gtgatctcgt cttgctcata gcgttttcgg tcgatttcta tcgcgcctga
     6241 ggtcggcgac agcgagccgg tcagcatccg gatggtcgtg gtcttacccg ccccgttggg
     6301 tcccaagaaa gagtaaattt ctccgcttcg aacgtgaagg tagagattgt cgacagcggt
     6361 gaggttgccg aattttttcg ttagggcttt tgctatgatc aaaaaaaggc ctctctttcg
     6421 cgaaccgtcc gtcttaggaa ttcaacgctt catggcattg tttcaacaaa tctattatcg
     6481 gtaacccttg tgggcaaagg gtttcgcatt ttccgcaggc gacacaacga tcggctttgt
     6541 gtttggacga aaagagagtg cgatattggg ttatggcggt ttccatcacg ccgaacatct
     6601 tcccttcgtt atagagtgaa aagatgtcgg ggattgcgac cccttccgga cacggttggc
     6661 agtacccgca ttcggtgcac gggatctgcc ggtagctctg gaaagcttcg actgcttttt
     6721 tgatggcttc attttctttt tgcgcaaagg tattggcttt catctggttg gcgactttga
     6781 cgttttcttg cgcctgagcc atcgtgctca tcccgctcaa gatcaaggac acttccggaa
     6841 agttcgcgac ccagcgtaaa gcccattcag ctggggacca ggtcggttgc gcctcggata
     6901 gtatcctacg tatttcagga gaaggattcg cgagctttcc ccctttgatc ggttccatca
     6961 cgatcagggc catacccttc gcatgcgcca aacgaaggcc acgtagcccc gcttgcacct
     7021 ctgtgtccat atagttaaac tggatctgac agaaatccca atcgtaggtg tttacgatcg
     7081 actcaaacgc cgggtaatca tcgtggaaag agaatccgaa gaatcgaatt tcgccggctt
     7141 tccgcttctc ttccaagaac tcgagaacat cgagtccgtt gatcagatcc catcgctttt
     7201 gattcaacgt gtgcagtaag tacatatcga tgcgatcagt accgagcttc gttttttgct
     7261 cttgaaaaaa cctctcgaag tcggcttttt cctttaccag ataggtcggc atcttggtcg
     7321 ctaaaaaaac tttgtcacga taaccgtcgc ctaaggcctc tcgtgtgaaa tgctcgcttt
     7381 gtcctttatg gtaaccgtaa gccgtgtcga tgtaattgac gccctgatcg atggccattc
     7441 gaaccatccg cgtcgcttcg gcctt
//
LOCUS       DAOPXN010000108         7447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9917_length_7447_cov_6.676136, whole genome shotgun sequence.
ACCESSION   DAOPXN010000108 DAOPXN010000000
VERSION     DAOPXN010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9917_length_7447_cov_6.676136"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="PKM49_06815"
     CDS             complement(<1..1445)
                     /locus_tag="PKM49_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82561.1"
                     /translation="MGIVYQKNKQTGITYAYENEAYWDKEKKQSRARRKLIGKVDPAT
                     GEIIATKPYKKRTEPVPSTPVKPGPVAIGALKRSFYGATYLFDQIGKTLGITEDLKAC
                     FPDTYKQILSVAYFLILEEDNPLSRFSHWQRYHIHPYGGDLPSQRSSELFQSIEESGR
                     TLFFQKQGKRRLEKEYWAYDTTTISSYSEALKQVKQGRNKENDRLPQLNLALLFGEDS
                     GLPFYYRKLPGNISDVSTVRTLLDELDALGYKKVKLVFDRGFYSLKNVNALFADHRKF
                     IVGTKLNLTYVKEVLDEERENLKLWTHLQGQFGVYGLCRTIEWEYEQPRPYEGDVLTG
                     KRRAYLLLFYNPEKAAKDQIEMNEHLTRLQNDLLAGKRREEYGKEYDTYFSVTQTPKR
                     GCKVEPKQEAMREAVKNYGYFALLSNEVKDPFDALSLYRSKDIVEKAFGNLKERLNFG
                     RMQVSSELSLNGKLFVEFIALIYLSYVKKRM"
     gene            complement(1621..3144)
                     /locus_tag="PKM49_06820"
     CDS             complement(1621..3144)
                     /locus_tag="PKM49_06820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82562.1"
                     /translation="MRKLRIYVCEHYLPDYFEAIKREGLQNVNVEGFPALCSCGQGHA
                     QNKEQVFQMAEDGTDSIVFCDQFCEVLELLPHSPNIRIAPSEYCFSHLTSPEVIQKLL
                     DEGGYFIGSGWLNNWEARLKELGFDQKTAQAFFREISKKLVYFDTGADPDAKEKMAKL
                     SEYLNLPAQIIPYDLKELQSYIKGFYNEWSEGQESNENETAVLDSESRNQLLQSQVAE
                     YAMALDLLGKIGEYETREKMLEGIQEVFTSVLGAERFIFYSPKDDLSHLPSQVRALMK
                     EPDAQFYFDEAERRFFIVVKHARQIHGIIEAGDFLFPKFINHYLNVALNLSNICGLML
                     TNIERHEESVKMALLDPLTGIYNWRYATGVLQTEVYRSSRTGRAFSLIMLDIDHFKMI
                     NDRFGHQKGDIALRAIAEIVKKRLEKTDVFTRRSGDEFLILLSDTSGEQAALLAEDLR
                     LSVAQAKIPDVDHLTASFGVSEYRAGDTVDSVVHRADEQMYRAKQAGRNRVCSEEFV"
     gene            complement(3155..4195)
                     /locus_tag="PKM49_06825"
     CDS             complement(3155..4195)
                     /locus_tag="PKM49_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007193568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HNY82563.1"
                     /translation="MNAQPMSSMNRVLTTISHKEPDRVPLFLCLSFYGAKELGMTVPE
                     YFKKPENVVAAQLAMREKYQNDCLYSFFYASIELEAFGSETIFVDDGPPNAGAPVLRT
                     IDDVFRLEPPEIDTSPGLTRVLRATEMLKERVGNEVPIIGIVMSPFSLPVIQIGFEKY
                     LEMIYFRKDAFERLMEVNDSFCAAWANAQLAAGATAIGIFDPLSSPSLIEKPTYIRTG
                     YPVAQRFISKIKGATAIHLASGLTLPVVREIAETGAAILGFGVADDPVALKKEAEGRI
                     CLLGNLNGLEMTHWTKERAHRTVKTVIDQFGKGGGFILSDGHGEIPWQVPEEILIAVS
                     EAVQLYGNYPLE"
     gene            complement(4192..4830)
                     /locus_tag="PKM49_06830"
     CDS             complement(4192..4830)
                     /locus_tag="PKM49_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562727.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNY82564.1"
                     /translation="MYKFNGLAEDFEQALLNTDRVVATSIFRRYAEATEGFSHIERLV
                     VNTLDQIGEKWERGIVSLAQVYMSGRICEELIDEYLPVAAVKRRESPRIAIAMLIDQH
                     ALGKRIVTSVLRAAGYNVIDFGQGLTSESLVEKTIESRIEILLISTLMYRSALKVREL
                     RDRFERAGYPVKIIVGGAPFRLDEGLWERVGADAGSRKASDIFALLEKEVLS"
     gene            5812..7281
                     /locus_tag="PKM49_06835"
     CDS             5812..7281
                     /locus_tag="PKM49_06835"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HNY82565.1"
                     /translation="MELIWAGLIILATFSTKITTKIGIPGLLIFLGIGMVFGSDGLGW
                     LYFDNMMLARQISDIALIIILFEGGYTIKKNLLKTALKPALSLATLGVVITGVVTALF
                     IRFFFHMEWLTAMLLGSIISSTDAAAIFSILRGKALPPKVTATLQVESASNDPMAIML
                     TVTIIGLINGAQQSAWLSVLSFLWQLGGGAFIGWLIFLVGKWLFNSLRAGSGGFYYVL
                     IIGVCFLAFGFAKIVYSNGFVAVFFAGVLFGNARFAFKGGVSHFLEGISAFAQVLVFL
                     ILGLLVFPRQLLFSWEEGLWIAIILMFVSRPVAVFLCTLFTRFTFKEKVFIAWSGFKG
                     AVPIVLGTYPVVAGLDPDSRLFNIVFFAVLLTTLLEGSTLDWVGRSLGLLKESAPKPL
                     VSMELMALENANFELIEISLSETDFASKKLIRDMEFPPDVSITAIVRDNEVIPAKGNT
                     RLLSGDLLFVLTPTTLSETLPDYFSCLNSSPCPPEASDT"
BASE COUNT         1680 a         1965 c         1750 g         2052 t
ORIGIN      
        1 tgcattcgct tcttcacata ggacaggtat attaatgcga taaattccac aaatagcttc
       61 ccgttcaagg ataattccga ggaaacctgc attcttccga agtttagccg ctctttcagg
      121 tttccgaatg ccttctcgac gatatccttg ctccggtaaa gggacagcgc gtcaaacggg
      181 tcttttacct cgttcgagag taaggcaaag tagccgtagt tcttcacagc ctctcgcatc
      241 gcctcttgtt tgggttcgac cttacacccc cgcttgggtg tctgagtcac cgagaagtag
      301 gtgtcgtatt ctttgccgta ctcctcccgt cgtttcccag ccagaagatc attttgtagc
      361 cgggttagat gttcgttcat ttctatctgg tcctttgccg ccttctccgg attgtagaat
      421 aagagtaggt atgcccgcct ctttcctgtg agcacgtctc cctcgtaggg ccggggttgt
      481 tcgtactccc attctatggt ccggcacagc ccatacacac cgaattggcc ctggagatgg
      541 gtccatagtt tcaagttctc tcgctcttcg tccaggacct ccttcacgta tgtcaagttc
      601 aacttcgtcc caacgatgaa ctttcggtga tccgcgaata aggcgttgac gttcttcaga
      661 gagtaaaacc ctcgatcgaa gacgagcttg acctttttgt accccagcgc atcgagttca
      721 tctaacagtg tccgaaccgt cgacacatca gagatattcc ccgggagctt ccgatagtag
      781 aagggtaagc ccgaatcttc cccgaacaac aaggccaagt tcagctgggg gagtctgtcg
      841 ttctctttgt tccttccttg cttcacttgt ttgagcgctt ccgagtaact ggagatcgtt
      901 gtggtgtcat aggcccagta ttccttttct aacctgcgct tcccttgttt ctggaagaac
      961 agcgtcctcc cactttcctc tatcgattgg aacagttcgc tgctcctttg ggaggggaga
     1021 tctcccccgt acgggtggat atggtatctc tgccagtgtg agaatcgact gagcgggttg
     1081 tcctcttcca atatcaggaa gtaggcgaca gagagaatct gtttgtaggt atccggaaaa
     1141 cacgctttca agtcctccgt gataccaagg gtcttcccga tttggtcgaa gagatacgtg
     1201 gccccataga aactccgctt cagggcaccg atcgctaccg gacccggttt aaccggggtt
     1261 gatggaacgg gttcggttcg tttcttgtag ggtttggtcg cgatgatttc ccctgttgct
     1321 gggtccactt tcccgatgag ttttcttctc gctctcgact gctttttctc tttgtcccag
     1381 tacgcttcgt tttcatacgc gtatgtgatt ccggtttgtt tgttcttctg gtaaacaatg
     1441 cccataggta tccttcctcg tcatatatta taatatataa cgatggattt gtcaatacct
     1501 tttcttcgta ctccccttcc tgtcttccta actatccctc tttcatcgct aggctttctg
     1561 ggaatccagt ttaatatgcg atgatcaatc aaactctcga gcccttgctt tgttcggcac
     1621 ctacacgaac tcttcgctgc aaacacgatt tctgccggct tgtttagcgc gatacatctg
     1681 ttcgtccgct cgatgcacga cagaatcaac ggtgtctccc gcgcggtatt cggagacgcc
     1741 gaagctagcc gtcagatgat ctacatcagg tatctttgct tgagcgacag aaagacgaag
     1801 atcttcagct aaaagggctg cttgttctcc gctcgtatcg ctcaacaaga tcaaaaactc
     1861 atcgccgctt cgtcgggtga agacatcggt tttttctaag cgttttttta ctatttccgc
     1921 aatcgctctg agcgcaatat ctcccttctg atgtccgaat cggtcgttta tcattttgaa
     1981 atgatcaata tccagcataa tgagagagaa ggctcttccc gttcgcgaag aacgatagac
     2041 ctccgtctgt aatacccccg tggcgtatct ccaattgtat atccccgtta gggggtctaa
     2101 taaggccatc ttaacgcttt cctcgtgccg ttcgatgttg gtgagcatca atccgcagat
     2161 attggagagg ttcaacgcga cattgaggta atgatttatg aattttggaa acaaaaagtc
     2221 gcccgcttcg atgattccgt gaatctgacg cgcatgtttc actacgatga agaaccgccg
     2281 ttctgcctca tcaaagtaaa actgcgcatc gggctccttc ataagcgcgc gaacttggga
     2341 aggcagatgg gaaagatcat ctttgggtga atagaatata aatcgttcgg cgcccaacac
     2401 agaggtgaag acctcctgga tgccttccaa catcttctcg cgggtttcgt actctccgat
     2461 ctttcccaac aggtcaagag ccatcgcata ttctgctact tgcgattgaa ggagttggtt
     2521 tcgactttca gaatctaaaa cggcggtctc gttttcgttc gactcttggc cttcactcca
     2581 ttcattgtaa aaccctttga tataggactg aagctctttc aagtcgtaag gtataatctg
     2641 ggccgggagg ttcaagtact cggacaattt cgccattttt tccttcgcgt caggatcggc
     2701 tcctgtgtcg aaatagacca attttttgct tatttcccta aaaaacgctt gagccgtctt
     2761 ttggtcgaaa cccaattctt taagtctagc ctcccaattg ttaagccaac ccgacccgat
     2821 gaagtacccg ccttcatcga gaagtttctg aatcacctcc ggggaagtta aatgcgaaaa
     2881 acagtattcc gatggagcga tcctgatatt cggactgtgc ggaagcaatt ctaaaacttc
     2941 acaaaactga tcgcagaaaa cgatggaatc tgttccatcc tctgccattt gaaagacttg
     3001 ttctttgttc tgggcgtgcc cctgcccaca actgcatagg gcgggaaacc cctccacgtt
     3061 tacattttgg agaccttctc gcttaatcgc ttcaaaatag tcgggaagat agtgctcgca
     3121 gacgtaaatt ctcagtttgc gcaaaatcaa cctctcattc caacgggtag ttcccgtaga
     3181 gttgaacagc ttcagaaacg gctatcagga tctcttcagg gacttgccag ggtatctcac
     3241 cgtgaccatc ggataagatg aatccgccgc ctttcccgaa ctggtcgatc acggtcttga
     3301 ccgttcgatg cgcccgctct ttcgtccaat gggtcatctc caacccattc aggttcccga
     3361 gcaggcagat gcgaccttca gcctctttct tcaaagcgac gggatcgtca gccactccga
     3421 atcctaggat cgctgctccg gtttcggcga tttcacgaac gacgggtaag gtgagtccgg
     3481 aggccaggtg gatagccgtt gcccctttta ttttcgagat aaagcgttgg gcaaccgggt
     3541 atccggttct tatataagtc ggtttttcta tcagtgacgg agaggagagc ggatcgaaaa
     3601 taccgatcgc ggtcgcgccc gcagccaatt gggcgttggc ccaagcggcg caaaaagaat
     3661 cgttgacttc catcagtctc tcgaacgcgt cttttcggaa atagatcatc tccaaatact
     3721 tttcaaatcc aatttgtatc acaggaagcg aaaacggcga catcacaatc ccgatgattg
     3781 ggacttcgtt tcccacacgt tctttcaaca tttcggtcgc gcgtaggaca cgcgttaaac
     3841 cggggcttgt gtcaatttcc ggaggctcca gccgaaagac gtcatctatg gtcctaagaa
     3901 ccggagcgcc cgcattagga ggtccgtcat cgacgaagat cgtttctgat ccgaaagctt
     3961 ccagctcgat cgaagcgtaa aagaaagaat aaagacaatc gttttggtac ttttcacgca
     4021 tcgcgagttg tgcagccacc acgttttcgg gctttttaaa gtattcgggc acggtcatac
     4081 cgagctcttt cgcaccataa aaggacaagc acaaaaaaag cgggactcgg tcgggctctt
     4141 tgtgggaaat cgtcgtcagt acgcggttca tcgagctcat aggttgagcg ttcaactcag
     4201 cacctccttc tctagcaggg caaagatatc ggaggctttc cgggatcccg cgtccgcgcc
     4261 tactctttcc cataacccct cgtcaaggcg aaacggcgcc cctcctacga tgatctttac
     4321 cggatagccc gctcgctcga aacgatcccg caactccctg actttcaacg cagagcggta
     4381 catcaaagta gaaataagta aaatttctat tcgactttcg atagtttttt ccacgaggct
     4441 ttccgaagtc agtccttgtc caaagtcgat tacgttgtac ccggcagctc tcaacactga
     4501 cgtcacgatt cgtttgccca atgcgtgttg atcgatcagc atcgcgatcg cgatccgagg
     4561 agactctctc cgttttactg ctgccacggg aagatactcg tcgattagtt cttcgcaaat
     4621 ccgcccgctc atatacactt gagccagaga aacgatgcca cgttcccatt tttcgccgat
     4681 ttgatcgagc gtattgacga caagacgttc aatgtgagaa aacccttcgg ttgcctcagc
     4741 gtatcggcga aagatcgaag tagccaccac ccgatcggta ttcaagagcg cctgttcgaa
     4801 gtcctcagct aagccattaa atttatacat ccttaaactc tcaccttccc tcttactcgt
     4861 ataaaaacac tccttgatat acatccagta atgtccacat tttatcgcga tgaaacctta
     4921 ccccgtatta tacacgattg ggtgtaaaaa aagttagctg aggtggataa agctaagggg
     4981 tgatatgact aagaggttca tcactggcta aatggtcttt atggctctgc gattcgccct
     5041 ttgcattttt atacgacggt gggacggaca aaaagcgtat cgcgccgtcg cgatgagcct
     5101 gtgagtaatc gcggggtaac caacgggacc ccgcgatccg ccttttgtta tttttcgaac
     5161 gatcagcccg cgtaaaagcg tatcgcgctg tcgctaaaag aaggagagct tatgcagcga
     5221 tgaccttgta tgtgatcacg ggctaaccag cgggctggct tcggaagccc ccgaagtctc
     5281 gcgctactaa cggatcttcg taatacgcgc tgctgttttt ctgttttcac aaaccctgcc
     5341 aaactaaagt ttggcgttcc cagaaaacgg ctcttcaaca atttacaaga cgtacttaat
     5401 taccatcttc tatcgtaata attctgtaga cccttagacc gtttcgctaa tccgctctaa
     5461 tcgcataatc gggcatcccg cgggctattt ttactcctct tatgttagtt tgtatttctt
     5521 ggatgatcag tgtgtttcaa gtaatgtatc gaagagccca gaaaacatat cgcccttacg
     5581 tacggcgttt ccaaggcgtt cccaatgagt tcccaggcgt ttcgcaggct ttcacaagcc
     5641 tatcgcgcag ttatgaaaaa aagtggcgct tacgccgcga tgactacacg agacaaaagc
     5701 atacggtcga atctggtctt gtttcgtgat tgcatcccag aacttgatcc acacaagcgt
     5761 ggcattttat ttctgaaagt ggtaaaatcg acgggtaaac ggaggtgtgc cgtggaattg
     5821 atctgggcgg gtttaatcat tttagcgacg ttttcaacga aaatcacgac aaaaatcggt
     5881 atccccggtt tactcatttt cttgggtatc ggcatggtct ttggaagcga cggactggga
     5941 tggctctatt tcgataatat gatgctggct cgccaaatca gcgatatcgc cttaattatc
     6001 atcctattcg agggcgggta cacgattaag aagaatctct tgaaaacggc tctaaagcct
     6061 gcgctatcct tggcaaccct gggggtcgtg atcaccggcg tcgtcaccgc gctgttcatt
     6121 cgctttttct ttcatatgga atggctcacc gcgatgctcc tcggttcgat catctcctct
     6181 accgacgcgg ccgctatttt ttcgattttg aggggaaagg ccttgcctcc gaaagtcacg
     6241 gcgacattac aagtagagtc ggcctctaat gacccgatgg ctattatgtt gaccgtcacg
     6301 atcatcgggt taatcaacgg cgcccagcag tccgcgtggc tctcggttct ctctttcctt
     6361 tggcaactcg gcggcggggc gttcattgga tggctgatct tcctggtcgg aaagtggctc
     6421 ttcaactcct tgagggcggg gagcggggga ttctactatg tgttgatcat cggcgtctgt
     6481 tttttggcct tcggtttcgc gaaaatcgtc tactccaacg gatttgtcgc tgtgttcttc
     6541 gccggggtct tgttcgggaa cgcgcggttc gccttcaaag gaggggtttc tcacttcttg
     6601 gagggcatct ccgctttcgc gcaagttctc gtctttctta tcctcgggtt gttggtgttt
     6661 ccgagacaac tgttgttctc ttgggaggaa gggttatgga tcgccatcat tctgatgttc
     6721 gtctcccggc cggtggccgt cttcctttgc acactgttca cccgttttac tttcaaagaa
     6781 aaggttttca tcgcttggag cggcttcaaa ggcgcggtgc ctatcgtcct cggcacttac
     6841 cctgtcgtcg cagggttaga tccggattct cgccttttca acatcgtgtt cttcgccgtc
     6901 ttgctgacca ccttgctgga aggctccacc ctggattggg tggggcgttc cttgggactc
     6961 ctcaaagaat cggcgccgaa accgctggta tcgatggagt tgatggcgct ggaaaacgct
     7021 aacttcgagc tcatcgaaat ctccttaagt gaaacggact tcgcttcaaa aaaattgatc
     7081 cgagatatgg agtttcctcc cgatgtctcc atcacggcga tcgttcgcga caacgaagtc
     7141 atccctgcca aagggaatac ccgactccta agcggagacc tgcttttcgt cttaaccccc
     7201 acgacgctct ccgaaaccct cccggattac ttttcctgct taaattccag cccctgtcct
     7261 ccggaggctt ccgacacgta accgggcggt agcgggactc caccggagaa ccgatagttt
     7321 cgccatatca tccccacagc gcgagagaac cccgctttct cgtaaaagtc gaccatatcc
     7381 acatcacaga tccctgactt tgtcacgggg tagggaaaaa tagggggtgc tgaaacgaaa
     7441 acagagg
//
LOCUS       DAOPXN010000109         7439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9930_length_7439_cov_6.467768, whole genome shotgun sequence.
ACCESSION   DAOPXN010000109 DAOPXN010000000
VERSION     DAOPXN010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9930_length_7439_cov_6.467768"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PKM49_06840"
     CDS             <1..405
                     /locus_tag="PKM49_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY82566.1"
                     /translation="KDLIQAGIIHKMQKLIGIQTESCNPLASAWEQQSAKPISIVLQP
                     TVAEGIAIAQPVHGKHILSVVRETGGALLSVTEEAILRNWREMAKKGFFMELTSATAF
                     AGIERVFESESRPEGTTVSLITGHGLKSIGHF"
     gene            complement(1092..2513)
                     /locus_tag="PKM49_06845"
     CDS             complement(1092..2513)
                     /locus_tag="PKM49_06845"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HNY82567.1"
                     /translation="MEKRIDERIDILIQEMVKEIGQAIRIPSVQGPAEAGAPFGRDVA
                     RALEFALSSGEKKGFVVKNVDNYAGHIEFGSSGRLYAALGHLDVVPVEKEGADWSVPP
                     FEGTVKDGAIWGRGASDDKGPTFAALYALYALKLLGVEPKNRIRVILGTNEETDWGCV
                     QHYFQKEAYPDFAIVPDSVFPGVFAEKGILRLDMRAPLCPAGPTDPTPIVSNRIFIEG
                     GTAPNVVPKETRLRVISRNPEMIDQMLRNFRPGNQATLTWTRNPTGEFEILFTGAPFH
                     AARPEGGINSIAACLDFCRSLGLAPLLKERLNRLWDAIGYDYSGKNAGISGQDGLSGA
                     LTLNLGTIACDERELRVTLDIRYPVFFDKGRVFKQIESAFRDFSIQESGSLAPLYVDP
                     KGQRMRQLLRIYEDFTGEAAPPKAIGGGTYARAVPLGVSYGALFPGEESYAHRANERK
                     SIASLTRACKIYARVFYEWLTES"
     gene            complement(2557..5058)
                     /locus_tag="PKM49_06850"
     CDS             complement(2557..5058)
                     /locus_tag="PKM49_06850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNY82568.1"
                     /translation="MNRLTPEEWIKKAEACKKSGEKRLAAACYLRAFRLRIYDESDIE
                     SANEALREALHVLEPQEISETMVAKRYLLTFFTGKTPDPVDEKQIFRSERYFYLVLWR
                     YCFENNPEPGIALFRERWRDKRKELIETKKVYLLHASYLYAVLLNQSGEYERGLHLIK
                     HLVEAFSARSDLMSRQLTAQCYTTMANYYVLKNQSTIAEDLYRKAYDLAAPLGLNLYT
                     ANIYYEAASFYSTVYGVSGFLDITEKVLEVTKAVSASPLALHALYRIGYNSLYSGDLK
                     EFWSYVEQLKHHSRRYQRPDFLAKAYLLEGVFYLYNKQFGKAETIFRKGFQCAKSPQT
                     LNLLQRCRILNFMMLGKRHSAFEQVQKSFFDKEEYGFHSFLALIEAQTPEEIADAFQH
                     FDAHSPRWREESALIFYEKIAPVDPSGFVRFCQNMVTDFSRSKDRLPLTLVYEALGKF
                     YRVIGYEEQARFFLENAVTNYRRLGMNHAASVLSPTSDLRALSVKRFEQKVRRLVHQS
                     PDRQLKRDFSAYRQQYDQAVDELGVYRMTFDFLRGIDCHCDVGQLLDQILRYLCDTLR
                     AQNGFIGLFDQNRALNSSSYRTQRDSAPFPTERWADEGFLPPYLPGHIWKTMYLEQGQ
                     RMVISLTFGSPLKARYERLLAFFLDEVKPIFELLVRSTLTFGKSLIDSLTQLNNRWFM
                     EQRLQEEFEKSIRYRFPLSFIMADIDDFKRINDTFGHRIGDEALRIIGARFRQNIRKY
                     DIAARYGGDEFAIILPNTEPERAYIVAEKIRKSVGEITDMPFRLTISFGVAGTGRICF
                     ASTEELVAAADGSLYEAKAQKKNRTVVYDHTARNE"
     gene            6424..>7439
                     /gene="nrdD"
                     /locus_tag="PKM49_06855"
     CDS             6424..>7439
                     /gene="nrdD"
                     /locus_tag="PKM49_06855"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HNY82569.1"
                     /translation="MLSIQKRDGSIVPFEIGKIQSAIEKAFVATEMVYTGDILELLSL
                     RAIAKCQPDIENGIVHLEKVQDAVEKVLVEAGFVETAKAYILYRKQHEKIRNFDKNML
                     DFSRTVNDYLDRSDWRVRENSTVSYSIGGLILHNSGTVIANYWLNEVYDEEIAKAHRR
                     ADMHIHDLSMLSGYCAGWSLKQLIREGLGGVNGKITSAPAKHLSTLVNQMVNFLGVLQ
                     NEWAGAQAYSSFDTYLAPFVKADSLSYESTKQAIQSFIFGVNTPSRWGTQSPFSNITL
                     DWTVPEDLKNVPAIVGGKNQPFTYGDCQEEMDMVNRAFLEIMIEGDAANRGFQYPIPT
                     YNLT"
BASE COUNT         1659 a         2058 c         1966 g         1756 t
ORIGIN      
        1 aaagacttga tccaggcggg gatcatccac aaaatgcaga aactgatcgg catacaaacg
       61 gaaagctgta atccgttggc ctccgcttgg gaacaacaat ccgccaaacc catctccatc
      121 gtgttacaac ccacggttgc cgaagggatc gccatcgcgc aacccgttca cggaaaacat
      181 atcctgagcg tggtccgaga aaccggcggc gccctcctat cggtcaccga ggaggcgatc
      241 ctacgcaact ggcgagagat ggccaagaaa ggcttcttta tggaattgac ctccgccacg
      301 gccttcgccg gcatcgaacg cgtttttgag agcgagtcgc gcccggaagg gaccacggtg
      361 agcctgatca ccgggcacgg tctcaaatcg atcggccatt tctagagcgg gtataatatc
      421 gaagcgtaat aaaaaagatg agattacgcg gggtatcgcg gggtaagtgt tttttaacga
      481 ccccgcgatc cgccttttgt cctttttacg aagctctggg aacggcctgg gaacgccgga
      541 ctttagtccg gcatgtcttc tctttttccg ggaactccct gagagcgccc tgggaacgcc
      601 ggactttagt ccggcatgta atctatcttc tctccgtgtc ctctgcgttc tccgtgcgaa
      661 ccctgttttc tgggaacgcc ggactttagt ccggcatgta ttctatcttc tctccgtgtc
      721 ctctgtgccc tccgtgcgaa ccctgtttta tttttaaaac atgccaactt aaaagttggc
      781 gttcccagcc cacgaaacct cgtaatctgc cttttgtcct tttttcgaag ccctgagagc
      841 gccctgggaa cgccggactt tagtccggca tgtaatctat cttctctccg tgtcctctgc
      901 gttctccgtg cgaaccctgt tttctgggaa cgccggactt tagtccggca tgtattctat
      961 cttctctccg tgtcctctgt gccctccgtg cgaaccctgt tttattttta aaacatggca
     1021 acttaaaagc tggcgttccc agcctattga gaaagacgcg aggagctctc ggctctcggt
     1081 ttatagcgcc cctacgattc cgtcagccat tcgtagaaca cccgcgcgta tattttacag
     1141 gctctcgtga gcgatgcgat cgactttcgc tcgttcgccc ggtgcgcata ggattcttct
     1201 cccggaaaga gcgcgccgta actgacgccc aatggaaccg ccctcgcgta cgtgcccccg
     1261 ccgatcgcct tcggcggcgc ggcctctccc gtgaagtcct cgtatattct cagcagctgc
     1321 cgcatccgtt gccctttggg gtcgacatat aaaggggcga gagagccgct ttcctgtatg
     1381 gaaaaatcgc gaaaggcgct ctctatttgt ttaaagacgc gccccttgtc gaagaatacc
     1441 gggtatcgta tatccagggt gactcgtagc tccctctcgt cgcaggcgat tgtgcccaag
     1501 ttaagggtga gggcgccgga aaggccgtcc tgaccagaaa ttcccgcgtt cttccctgag
     1561 taatcgtaac cgatcgcgtc ccacaaacgg ttcaaccgct ctttcagtag cggggcgagc
     1621 cccaacgaac gacaaaaatc taagcaagcg gcgatactgt tgatgccccc ttcaggccga
     1681 gcggcatgga atggggctcc cgtaaacaga atctcgaact ctccggtcgg attgcgggtc
     1741 caggtgaggg tcgcttgatt cccaggccga aagttgcgca gcatttgatc gatcatctcc
     1801 ggatttcggc tgataacccg cagcctcgtc tcttttggaa cgacgttcgg ggccgtgccc
     1861 ccttcgatga aaatacgatt cgaaacgata ggggtggggt ccgtgggacc cgccgggcag
     1921 aggggggcgc gcatatcgag ccttaagata cccttctcgg caaaaacccc cggaaacacg
     1981 gaatccggga cgatcgcgaa atccgggtac gcttctttct gaaagtagtg ttgaacgcat
     2041 ccccagtcgg tctcttcgtt ggttcccaag atgacgcgta tgcggttctt cggttcgacc
     2101 ccgaggagtt tgagcgcata gagcgcgtag agcgcggcga aggtggggcc tttgtcgtcg
     2161 ctcgcgcctc ttccccagat ggcgccatcc ttgacggtcc cctcgaacgg gggcacagac
     2221 caatcggcgc cttccttctc caccggcact acgtcgagat gtcccagagc ggcatacagc
     2281 ctaccgctcg agccaaactc gatatgaccg gcatagttgt cgacgttctt gaccacgaag
     2341 ccttttttct cgccggagga cagcgcgaat tccaaggccc tggcgacgtc tcgaccgaaa
     2401 ggcgcgccag cttccgcagg cccttgaaca gagggaatcc gtatcgcttg cccgatctct
     2461 ttcaccatct cttggattag gatgtctatc cgttcgtcta tacgcttttc caagtctcct
     2521 cccgccgaat ttcggacgct gccttccttc gtcctgtcat tcattgcgcg cggtgtgatc
     2581 gtagaccacc gtccgatttt tcttttgcgc tttggcttca tagagggagc cgtcggccgc
     2641 agcaaccagc tcttccgtac tcgcaaagca tatccgaccc gttccggcaa cgccgaagct
     2701 gatcgtcaag cgaaaaggca tatcggtgat ctcccctacg ctctttcgaa tcttctcagc
     2761 gacgatatag gcgcgctcgg gttcggtgtt cggtaggatg atcgcgaact cgtcccctcc
     2821 gtatctcgcc gcgatatcgt acttacggat gttttgccgg aaacgggcgc cgatgattcg
     2881 aagggcttcg tccccgatcc gatggccaaa ggtgtcgttg attcgtttga agtcgtcgat
     2941 atcggccata atgaaggaga ggggaaagcg ataccggata ctcttttcga actcctcttg
     3001 caaccgttgt tccataaacc agcggttgtt caactgtgtc aaggaatcga taaggctttt
     3061 cccaaaggtg agggtgctcc ggacgagcaa ctcgaaaatc ggcttcactt catccaaaaa
     3121 gaaggccagc agccgttcat accgcgcttt caacggagag ccgaaggtta acgagatcac
     3181 catcctctgc ccttgctcca ggtacatcgt tttccagata tgcccgggga ggtagggagg
     3241 aagaaatccc tcatccgccc accgttcggt cgggaatggc gcgctatccc gctgggttct
     3301 gtaactggag ctattgagcg cgcgattttg gtcgaatagg ccgatgaaac cattctgcgc
     3361 ccgaagggta tcgcagaggt accgaaggat ttgatccagt agttggccca catcgcaatg
     3421 acagtctata ccccgaagga aatcgaaggt catccggtaa acgcctagtt cgtctacagc
     3481 ttgatcgtat tgctgtcgat aggcggagaa atctcttttc aactgacggt caggcgattg
     3541 atggacgagc cttctgacct tttgctcgaa tcgtttcaca gacagggccc tcaagtcgga
     3601 ggttggcgat aacacggaag cggcgtggtt cataccgagc cgccggtaat tggtcaccgc
     3661 gttttctaaa aaaaagcgcg cctgttcctc gtacccgatg actcggtaga acttcccgag
     3721 cgcctcataa acgagggtga gaggaagacg gtccttcgaa cgagaaaaat ccgtgaccat
     3781 attttgacaa aaccgcacga atccagacgg atcgaccggc gcgatctttt cgtaaaagat
     3841 cagggcgctc tcctcgcgcc atctcggcga atgcgcgtcg aaatgctgaa aggcatcggc
     3901 gatctcttcc ggcgtttgcg cctcgatcaa ggcgaggaac gaatgaaacc catactcctc
     3961 tttatcgaag aaggactttt ggacctgttc gaaggcgcta tgccttttac cgagcatcat
     4021 aaaattcaat atcctgcagc gctgaagcag gttgagggtc tgtggggatt tcgcgcattg
     4081 aaaccctttc ctaaaaatgg tttcggcttt cccaaattgc ttgttgtaaa gatagaagac
     4141 gccctctagc aaataggctt tcgccaaaaa atccggacgt tgataacggc gcgaatggtg
     4201 cttcagttgc tcgacgtagg accagaactc tttcaggtct ccgctgtaaa ggctgttgta
     4261 tccgattcgg tacaacgcgt gcaaggcgag gggactcgcg gagaccgcct tggtcacttc
     4321 gagcactttt tctgtgatgt ccaaaaagcc gctcactccg tagacagtag aataaaagct
     4381 cgcggcttcg taatagatgt tggccgtgta gaggttaagc cctaacggtg ccgccaaatc
     4441 gtaggccttg cgatacaggt cttcggcgat cgtgctttgg tttttcaaaa cgtagtagtt
     4501 ggccatggtg gtgtagcatt gagcggtcaa ttgccgtgac atcaagtctg aacgcgcgga
     4561 aaaggcttcg accaagtgtt tgatcagatg caatcctcgc tcgtattccc cgctttggtt
     4621 gaggagcacg gcgtacaagt aggaggcgtg gagcaggtag acctttttcg tttcgatcag
     4681 ctctttgcgc ttatcccgcc aacgttcgcg aaatagagcg atccccggtt ccgggttgtt
     4741 ttcgaaacag taacgccaca acaccaaata gaagtatcgt tcggagcgga agatctgttt
     4801 ttcgtccaca gggtccgggg ttttacccgt aaagaaggtt aaaagatacc gtttcgcgac
     4861 catcgtttcc gagatctctt ggggctccag cacgtgtagc gcttctctca acgcttcgtt
     4921 cgcgctttcg atatcgctct catcataaat gcgcaggcga aaggcgcgca aataacacgc
     4981 ggccgctagc cgtttttctc cgcttttctt acacgcttct gctttcttta tccattcttc
     5041 tggggttagt cggttcatca caaggaatct ccaaaccata atggctatgg aattatagca
     5101 ttttttaaag aaaaaataac caaaagtgtg tgtcaagtta tggggtgtac tcaagaaaca
     5161 aggacagaat cgatcgcgca gtttttagcc cacgtagtca tcgcggcgta agcgcccctt
     5221 cttttcgcga cggcgtgata cgctttagta tatgtcttga aaaacagggt tcgcacagag
     5281 ggcacagaga acacagagaa aagattgaag aaaactcgtg gggccaactt ttaagttggc
     5341 gggttttagg tttttggaat gtcatcgcgg tgtaggcgct tcttcttctc gcgacggcgc
     5401 gatacgcttt tgtacgggca gcgcggcgtc ttggcgggca tcgcggcgta agcgcccctt
     5461 cttctcgcga cggcgcgata cccggacaca ggcatcgcgg cgtaagcgct tcttcttctc
     5521 gtgacagcgc gatacccgga cagcatcgca gtgtaaagaa aacaaaaggg ggatcgcggg
     5581 gtcccgttgg ttaccccgcg ataacctcgt ttgggcatcg cggcgtaagc gcaccttctt
     5641 tttgcgacgg cgcgataccc ggactcaggc atcgcggcgt aagcgcttct tcttctcgcg
     5701 acggcgcgat acccaggcac aggcatcgcg atgtaaagat aacaaaaggg ggatcgcggg
     5761 gtcccgttgg ttaccccgcg ataacctcgt ttgggcatcg cggcgtaagc gcaccttctt
     5821 tttgcgacgg cgcgataccc ttttttacag ccggatcgct gggcttcgtc aggatcgcgg
     5881 ggacccttcg gttaccccgc gatgaccccg gtcgggtatc gcgacgtaag cacccctttt
     5941 tcatacgacg gcgtgatacg cttttgtgcg cgggcatcgc ggcgtaagcg caccttcttt
     6001 ttgcgacggc gcgataccct tttttacagc cggatcgctg ggcttcgtca ggatcgcggg
     6061 gacccttcgg ttaccccgcg atgaccccgg tcgggtatcg cgacgtaagc accccttttt
     6121 catacgacgg cgtgatacgc ttttgtacac gtcttaaaaa aaggagtttg cacgtcaact
     6181 ttcactcatc gatacgaatt tttaccttat atctatccct ctgacattca tgtcatcaca
     6241 atgtatcaag ccgagtcaga caaaaagtcc ggctttttta taaccggtat cagagcggtt
     6301 tttacgctat atatagtgga ttgttgattt gtaatactat atatagtata atccttgagc
     6361 tatttccgat aaaactctaa agagagaagc gcagcgaggg gaaatcctat gaagaaaaat
     6421 gacattttgt ccatacagaa gcgggacggc agtatcgttc cgttcgagat agggaagata
     6481 caatccgcga tcgaaaaagc tttcgtcgcg accgagatgg tctacaccgg cgatattttg
     6541 gaactgctct ccttgcgggc gatcgcgaaa tgccagccgg atattgaaaa cgggatcgtc
     6601 catctggaga aagtccagga tgcggtggaa aaggtcttgg tcgaagcggg tttcgtggaa
     6661 acggcgaaag cctacatcct ctaccgcaag caacacgaaa aaatccggaa cttcgacaag
     6721 aatatgctcg acttttccag gacggtcaac gactatctgg accgctccga ctggcgagtc
     6781 cgcgaaaaca gcaccgtttc ttactcgatc ggcggcctga ttttacacaa tagcggaacg
     6841 gtcatcgcca attattggtt gaacgaggtc tacgatgagg aaatcgcaaa agcccaccga
     6901 cgggcggaca tgcacatcca cgacctctcg atgctgtccg gttactgcgc cgggtggagt
     6961 ttgaagcagc tgatacgcga aggcctgggc ggggtgaacg gaaagatcac ctctgcgccg
     7021 gccaaacacc tttccaccct ggtcaaccag atggtgaact tcctcggcgt tctgcaaaac
     7081 gagtgggcgg gcgcgcaagc ctacagctcg ttcgacacct atttggcacc cttcgtcaaa
     7141 gccgacagcc tcagctacga atcgacgaaa caggcgatcc aatcgttcat cttcggcgtc
     7201 aacaccccga gccgatgggg cacgcagtcc cccttttcca acatcaccct cgactggacc
     7261 gttcccgaag acctgaagaa cgtgcccgcg atcgtcggcg ggaagaacca gcctttcacc
     7321 tacggcgact gccaggaaga gatggatatg gtcaatcggg cgttcctgga gatcatgatc
     7381 gagggagacg cggccaatcg cggcttccaa tacccaatcc cgacctacaa ccttacaaa
//
LOCUS       DAOPXN010000110         7409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_9985_length_7409_cov_5.389448, whole genome shotgun sequence.
ACCESSION   DAOPXN010000110 DAOPXN010000000
VERSION     DAOPXN010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9985_length_7409_cov_5.389448"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="PKM49_06860"
     CDS             complement(<1..1448)
                     /locus_tag="PKM49_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNY82570.1"
                     /translation="MARSNESLAQKREKLIGSFLHKHWYRYLIGILILLLVDVLQIYI
                     PGYIKTLVDSLESSAGDLAVVASSIQIILLISVGIFFSRFFWRWFIIGSSRMFEEFLR
                     NRLFSKLLSLSPSFYDRIKVGDIMARMTNDVSATRMMLSQGVIMLTDAIVLSVLSIYM
                     MITQVDWRLTLIGMIPLPFLGLIAVGFGRAIHHRFTAVQNTFSDITDLAQESLDGIRV
                     VKSYAIQPERNTRFGSKCAEYFAKSMNLVRVWGLFFPLIELFASLGTVFALFYGGHLI
                     ITGQISLGQFIAFTQYLGLLVWPMIAIGWVINIIQRGRASYKRLNWIVEQVSEVQEPV
                     TPEKADLSKQIEIRDLSFSYPGSEKLALKSLSLTIEKGKTVAFVGQTGSGKSTLGKLL
                     VKMYSVPVGKIFIGGKDINALSMKDIRDQVSYVPQETFLFSATIEQNIAFSHDVYSAE
                     EVQNYAKLAAVHDNIADFPEGYRTLVGERGITL"
     gene            complement(1554..2591)
                     /locus_tag="PKM49_06865"
     CDS             complement(1554..2591)
                     /locus_tag="PKM49_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNY82571.1"
                     /translation="MTSLSKRVDPAMWVILVFFLIFLFLFKLAPTIMGALVFGWYLSL
                     IIEVPARALSKIKGVSYRFGTVVSAIVLFSLIAYAFYSVFPILIEEGKKIFPMIQKAA
                     QNLNLKPLFSTGARIDQKAVDIVEDITTQFVSRFSELGVNILNTVIQHIPGAMTAIML
                     FIITASYFTTLLPMLKKNLWRFFPKSTLARSIEVMSALYNEVRHFITGQVMLAGIIGF
                     LVGLGMFFAQIPYALFLGFLSGITNFVPFLGVFVAGVPAVLLGFTNAGVPGALKALIV
                     LIIVNQLESWVLSPKIQGSRMKINWFVIIISVFICGSYFGAVGVLLAIPMLLFLRHYW
                     SAYMLELFKAL"
     gene            complement(2575..3159)
                     /locus_tag="PKM49_06870"
     CDS             complement(2575..3159)
                     /locus_tag="PKM49_06870"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNY82572.1"
                     /translation="MDKETLLREIIDRIATCTRCGLCEHRTQTVPGEGNLNARLFFVG
                     EAPGREEDLSGRPFVGAAGTLFTEILKSVKIDRKDVYIGNIIKCRPPDNRTPTMAEMD
                     ACAPFLLAQLQVVHPKLIVTLGASSLSYFLPEREIKITEMRGKRLQWEGGITIFPMFH
                     PSYLLRNASKAPGSPKALTWDDIREVRRIYDQSE"
     gene            complement(3230..3718)
                     /locus_tag="PKM49_06875"
     CDS             complement(3230..3718)
                     /locus_tag="PKM49_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HNY82573.1"
                     /translation="MLTFEVISLDCPEDCNLILGQSHFIKTVEDISELLVTSVPGIEY
                     GLAFNEASGPCLIRVEGNCAPLVDAARDNAQRIGAGHIFVLLIRKAYPINFLNGLKQT
                     QEVCSIYAATANPIQVIVAQTQQGRGIMGVVDGSSPKGVEGPEDIANRKSFLRKVTGY
                     KF"
     gene            complement(3721..5919)
                     /gene="oraE"
                     /locus_tag="PKM49_06880"
     CDS             complement(3721..5919)
                     /gene="oraE"
                     /locus_tag="PKM49_06880"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HNY82574.1"
                     /translation="MSLDPTKKLDVDEILTGLADYRPKRKGWHWREAHPSEKKEGSYR
                     YEEISKPLTQSLPLPAGRYFDNIDPQPQEIITAEIASGRFEDDLRRMRMAAWHGADHI
                     MVIRTLGQSHFDGLLEGTPEGTGGVPITRKEIRASRKALDLIEEEVGRPINFHSYVSG
                     IAGPEVAVLFAEEGVNGAHQDPQYNVLYRGINPIRSFVDAAVAKKLMASADILQIDGA
                     HNANASSKIAWKVMPELLVQHAINSSFSVRAGMKKSLISLSTVPPMSSPSPSFRLNFV
                     YALTIRELFKEFKFRAQMNTRYIEADLYDATRIHVLDAVLSRLTHADLQSTITPDEGR
                     NVPWHINSIRGVQTAKHTLIALDGIKRYVGPKMDALKDEMRDLKIRAILYMEDILENG
                     GYFEAVEKGFFVDSGYFPERNGDGIVRKKDGELGAGSIVPRERDYYAPVCHHYGYNAL
                     PAGVKKACDPIRGCTLDHPEKIQFLDELDENDNVEVRRAAVRESERKGLLKPEVEWKN
                     DGIIQLDITIPDNPDIAEAAALEIANRLGLEEPTVISKTVLHPAEGTYVELKARVPFF
                     VDRNTLRLPEKPVTLSEEEITRYVHENPLRVVAGTVGNDEHSVGIREILDIKHGGIEK
                     FGIKYTYLGTSVPVEKMIDAAIETNSQAILCSMIITHNDVHIDEMKKIHQLCIEKGVR
                     DRLLLIAGGTQIKNEIAIQSGMDAGFGRGTKGIDVASFIVKTLQGKKAGG"
     gene            complement(5916..6281)
                     /locus_tag="PKM49_06885"
     CDS             complement(5916..6281)
                     /locus_tag="PKM49_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HNY82575.1"
                     /translation="MKTDLFEKRKQELSRLTDEELNRKFWELSEKIVDPLVKLAYENT
                     TPSIERSVLLRMGFNSLEAKGFVDKCFERGLLGRGAGHVILKVAEELKRDYLAVGKEM
                     AEGKHWEVAEKSFKGVKIG"
     gene            complement(6477..7019)
                     /locus_tag="PKM49_06890"
     CDS             complement(6477..7019)
                     /locus_tag="PKM49_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNY82576.1"
                     /translation="MGYNRVNKTRNVRMGQSERIFYRGQLLPLNVVSSNRVGNVSISA
                     GSASLEISIHPYLKSRLTDGDVKKWVRLWLESEARRLIPIKVREWAEKTSLYPDKLRV
                     KDQRSRWGSCSSKGNINLNWRLIMAPETVMEYVIVHELCHLRFPNHSKSFWALVAHYL
                     PDFQMSKSWLRKNGQMLFLT"
BASE COUNT         1773 a         1960 c         1858 g         1818 t
ORIGIN      
        1 agcgtgattc cccgttcgcc cactagggta cgataaccct ccgggaaatc ggcgatgttg
       61 tcgtgaacgg ccgccagttt ggcgtaattc tgcacttctt cggcggagta gacgtcgtga
      121 ctgaaggcga tgttttgttc gatcgttgct gagaagagga aggtttcctg agggacgtac
      181 gaaacttggt cgcggatatc cttcatagat aaggcattga tgtccttacc gccgataaag
      241 atcttgccga caggcaccga atacatcttc acgagcagct tacctaacgt ggactttccg
      301 ctacccgtct gcccgacgaa ggccaccgtc ttcccttttt ctattgtcaa tgagaggctt
      361 ttcaaagcaa gcttttccga ccccgggtaa gagaaagaga ggtcacggat ttcgatttgc
      421 ttgctcaaat ccgctttttc aggggtaacg ggctcttgca cttcggagac ttgttccacg
      481 atccaattca accgtttgta agatgcccgc cccctttgta tgatgttgat cacccaaccg
      541 atcgcgatca tcggccaaac gagcagtccg aggtactggg tgaaggcgat gaactggcct
      601 aggctgatct gccccgtgat gatcaggtga cctccgtaaa acaatgcgaa caccgttccc
      661 aaagaggcaa atagctctat caatggaaaa aagagtcccc atactcggac caaattcatc
      721 gatttcgcga agtactccgc gcacttgcta ccgaatcggg tattcctttc tggctgaatc
      781 gcatagcttt tcacgacccg aatgccgtcc aatgactctt gggcgaggtc ggtgatgtcg
      841 gaaaaggtgt tctgcactgc cgtgaaccga tgatggatcg cgcggccgaa tccgactgcg
      901 atgaggccta agaacggcag cggaatcatc cctatgagcg tcagacgcca atcgacttgg
      961 gtgatcatca tatagatgct taagacgctc aagacgatgg cgtcggtcag cattatcaca
     1021 ccttgagaaa gcatcatccg tgtggcgctc acgtcgttcg tcatccgggc cataatatcc
     1081 ccgaccttga tgcggtcgta aaaagaaggt gagagcgaaa gcagcttaga aaacaaccgg
     1141 ttcctcaaaa actcttcgaa catcctcgag gaaccgatga taaaccaacg ccaaaaaaaa
     1201 cgggagaaga atatgccgac cgagatcaac aggatgatct gaatagacga cgcgaccact
     1261 gccaggtctc ccgccgacga ttccaggcta tctaccaagg ttttaatgta tccggggata
     1321 taaatctgca ggacatcaac caatagaagg atcaagatgc cgatcagata acgataccag
     1381 tgtttgtgca agaaactgcc gatgagtttt tccctcttct gtgccaaaga ctcattgctc
     1441 cgtgccattt gggtgctcct tcaagtcgca catttctact tccgggctgt attatagcac
     1501 ttcgtccgat aaaaaagaaa cggtaaccga aggagcgagg gggggttagg ggttcaaagc
     1561 gctttgaaca actcgagcat gtaagcggac caatagtgtc gtaagaacaa cagcataggg
     1621 atcgcgagca agacgcccac agctccgaag taggagccgc aaatgaagac ggagatgatg
     1681 atgacgaacc agttgatctt catccgtgag ccctgtattt tgggggaaag gacccagctt
     1741 tccagttgat tgacgatgat gaggacgatc aacgccttga gcgccccggg aactcctgcg
     1801 ttcgtgaagc ccaagagaac ggcgggaaca cccgcgacaa aaacgcctaa gaacggcacg
     1861 aagttcgtga tccctgagag aaatcccaaa aaaagggcat aggggatctg cgcaaaaaac
     1921 atccccaagc ctactaagaa gccgattata ccggccagca tgacctgccc ggtgataaaa
     1981 tggcgcacct cgttgtagag ggcgctcatt acttctatag agcgcgctaa ggtcgacttc
     2041 ggaaagaatc gccacaagtt tttcttaagc atcggcagaa gggtcgtgaa gtagctggct
     2101 gtgatgatga agagcataat ggcggtcatc gcgcccggaa tgtgctggat cacggtattg
     2161 aggatgttga cgccgagctc cgaaaaccgg gaaacgaatt gagtggtgat gtcttcgacg
     2221 atatcgacgg ccttctggtc tatccgcgct ccggtggaaa agaggggttt caagttcagg
     2281 ttttgagccg ctttttggat cattggaaag attttcttgc cttcttcgat caaaatcgga
     2341 aagacggagt agaaggcgta ggcgattagg gaaaacaaga cgatcgcaga aacgaccgtt
     2401 ccaaaacgat aagagacgcc tttgatctta gacagcgccc gagccgggac ttctatgatc
     2461 agagaaaggt accatccgaa gacgagggcg cccataatcg ttggggccag tttgaagagg
     2521 aataaaaaaa tcagaaaaaa taccagaatt acccacatcg cgggatcgac ccgtttactc
     2581 agactggtca tatatcctcc gcacctcgcg gatatcatcc caggttaagg ctttcggtga
     2641 acccggcgct tttgaggcgt ttctcaagag gtagctgggg tggaacatcg ggaaaatcgt
     2701 gattcctccc tcccactgca ggcgtttccc gcgcatctcc gtgatcttga tctcccgttc
     2761 aggtagaaag taagagagtg aggaggcgcc cagcgtcacg atcaatttcg gatgcacgac
     2821 ttgaagctgg gccaagagaa aaggcgcgca cgcgtccatt tccgccatcg tgggtgtgcg
     2881 gttatccggc ggtcggcatt tgatgatatt cccgatgtac acgtcctttc tgtcgatctt
     2941 caccgatttc aggatttcag tgaaaagcgt accggctgcg cctacgaaag ggcgcccgga
     3001 aaggtcttcc tctcttccgg gcgcctctcc aacgaaaaat aaacgcgcgt ttaaattgcc
     3061 ttccccggga accgtttgtg tgcgatgttc gcataatcca caacgcgtac acgtcgcgat
     3121 ccgatcaata atttctctta aaagggtttc tttgtccacg cacatcgatc ctttcgacac
     3181 aatctgggaa gatgttcgat actaggtgtc caaccggggt tcctcaccct tagaatttat
     3241 atccggtcac cttacgcagg aacgatttcc gattcgcaat gtcctctgga ccttccactc
     3301 cttttggaga ggatccatcg acaactccca ttatccctct accctgctgc gtttgcgcga
     3361 cgatgacctg aatcgggttc gcggttgccg cgtagatcga gcagacctcc tgcgtctgct
     3421 tcaacccgtt caagaaattt atcggatagg ctttgcggat caacaagaca aaaatgtgac
     3481 cggcaccgat acgctgggcg ttatcacgcg cggcgtcaac caggggcgcg cagtttccct
     3541 ccacgcgtat taaacacggt ccggaagctt cgttgaaagc caagccgtat tcgataccgg
     3601 ggacggaggt gacgagcaat tcggagatat cttccaccgt tttaatgaag tgcgactgtc
     3661 caaggatgag attgcaatcc tcgggacagt ccaatgagat cacttcaaag gtaagcaaag
     3721 ttaaccgccc gcctttttcc cctgaagggt cttgacgata aagctggcga catcgattcc
     3781 cttcgttcct cttccaaaac cggcgtccat acccgactga atggcgatct cgttcttaat
     3841 ctgcgtgccg cccgcgatca gcagcaaccg gtcacgcaca cctttttcta tgcagagttg
     3901 atggatcttt ttcatctcgt cgatatgaac atcgttatga gtgatgatca tactacaaag
     3961 aatcgcttgg gaattcgttt ctatcgcggc gtcgatcatt ttctcgaccg gcacgctggt
     4021 ccctaaatag gtgtatttaa tgccgaactt ctctattccg ccgtgcttga tatccaaaat
     4081 ttctctgatt ccgacagagt gttcgtcgtt ccccaccgtt ccggcgacga cccgcagcgg
     4141 attctcgtga acgtaccgtg tgatctcttc ctccgatagc gtgacgggtt tttcaggcaa
     4201 tcgcaaggtg ttccgatcga cgaaaaacgg cacgcgggcc tttaattcga cataggttcc
     4261 ctcggccggg tgcagcactg tttttgagat gaccgtaggc tcttccaatc ccaaacggtt
     4321 ggctatttcc aaagccgcag cttcagctat atcggggtta tcggggatcg tgatgtccaa
     4381 ctgaatgatc ccatcgttct tccattcgac ttccggtttg agcagaccct tgcgttcgga
     4441 ctctcgaacg gcggcgcggc ggacttctac gttgtcgttt tcgtccagtt catccaagaa
     4501 ctggatcttt tccggatgat ccagggtgca tcctcgaatg gggtcgcacg cctttttaac
     4561 gccagcggga agcgcgttat aaccgtaatg gtggcaaacg ggcgcgtaat agtcgcgttc
     4621 tcgaggtacg atgctgccgg ctccgagttc gccgtctttt ttccttacga tgccatcgcc
     4681 attcctctcg ggaaagtatc ccgaatcgac gaaaaagcct ttttcgaccg cttcaaagta
     4741 gccgccgttt tctaagatat cctccatata aaggatcgcg cggatcttca agtcacgcat
     4801 ctcgtccttc agcgcgtcca tcttaggccc gacgtaccgt tttataccgt ccagtgcgat
     4861 taaagtgtgt ttggccgtct ggactcctcg tatcgaattg atgtgccagg ggacattacg
     4921 accctcgtcc ggggtgatgg tggattggag gtcggcgtgc gtcaatcgac tgagaacggc
     4981 atccagaacg tgtatacggg ttgcgtcgta taggtccgct tcgatgtaac gcgtgttcat
     5041 ctgagcgcgg aacttgaatt ctttgaagag ttctcgaatc gtcagcgcgt aaacgaaatt
     5101 gagccggaaa gacggagagg gagaggacat cggggggacc gtcgataaag agatcaacga
     5161 tttcttcatc cccgcgcgaa cagaaaacga gctgttgatc gcgtgttgaa ctaggagttc
     5221 cggcatcacc ttccaggcga tcttcgatga ggcgttcgcg ttgtgcgcgc catcgatctg
     5281 cagaatgtcg gcgctggcca tcagtttctt cgctacggcg gcgtccacaa aagaccggat
     5341 cgggttgata ccccgataca agacgttgta ttgcggatcc tgatgggctc cgttcacgcc
     5401 ctcttcggcg aacaagacag ccacttccgg ccctgcgata ccggagacgt aagaatggaa
     5461 attgatcggc ctgcctacct cctcttctat caagtccagc gctttacgac ttgcgcggat
     5521 ttcttttcgg gtgatcggca cccctcccgt tccttccggc gtcccttcca acaacccgtc
     5581 gaaatgggac tgtccgaggg tccggatgac cataatgtga tcagcgccgt gccaggcagc
     5641 cattctcata cggcgaaggt catcttcgaa tcttccggaa gcgatctcgg ccgtaatgat
     5701 ttcctgaggt tgcgggtcga tattgtcgaa gtatcgtccg gccggcaaag ggagcgattg
     5761 ggtcaggggt ttcgatatct cttcgtatcg gtaactcccc tcttttttct cgcttggatg
     5821 cgcttcacgc cagtgccaac cttttcgttt cggacgatag tctgctaaac cggtcagaat
     5881 ctcgtcaaca tccaattttt tcgtcgggtc taaactcatc cgatcttcac tcctttgaac
     5941 gatttttcgg caacctccca gtgtttgcct tccgccattt ctttaccgac ggccaagtag
     6001 tcccgtttca gctcttcggc cacctttaaa ataacgtgac cggctcctct tcccaacaag
     6061 cccctttcaa agcacttgtc gacgaaccct ttcgcttcca aagaattgaa ccccatccgt
     6121 agaagaacag atcgctcgat cgagggcgtc gtgttttcat acgcgagttt gacgagcggg
     6181 tccacgatct tttccgacaa ttcccaaaac tttcggttca gctcttcgtc tgtcaagcgt
     6241 gacaactctt gctttcgctt ctcaaataga tcggttttca agttttcctc cttaagatgc
     6301 aagatttttc cgaagatcgg aatgggtgtt cgcaccttcg cagtcgatac catcctcgta
     6361 gggaggcatc cgatggtgaa ggaaccgtag gctcatccct taggaagacc ccgtcgaagg
     6421 tttaggccag tgatcctttc tattcggacg atgaactatt atacctgatt aaattattaa
     6481 gtcaagaaga gcatttgacc gttttttctt aaccagcttt tactcatctg gaaatcgggg
     6541 aggtagtggg caaccagcgc ccaaaaagat tttgagtgat ttggaaaacg caaatggcag
     6601 agttcgtgaa cgatcacgta ttccattact gtttctggag ccatgatcaa cctccaattc
     6661 aggttgatgt tcccctttga agagcagctc ccccaacgag aacgctggtc tttgacccgt
     6721 aatttgtcag gatagaggga ggtcttctcc gcccattctc tgaccttaat cggaatcaac
     6781 cgccgggcct cgctctccaa ccataacctg acccatttct tcacgtcccc atctgtgagc
     6841 ctactcttaa gatacgggtg gatcgagatc tccaaagagg cggatcccgc cgaaatagag
     6901 acgtttccga cccgattgct ggaaacgacg ttcagtggca gcagctgtcc ccggtaaaaa
     6961 atgcgttccg actgtcccat acgtacattc ctcgttttat ttaccctatt atatccgatt
     7021 ttgctgaatt tagagatacc tggtgtgcgt caaattatgg ggtgtaatca agaaacaagg
     7081 acagaattga tcgcgagagt tttagcccat atagtaatcg cggcgtaagc gtctcttctt
     7141 ttcgcgacgg cgcgatgcgc ttttaagaaa ggtaaattgc ggatcgccgg gtcataaaaa
     7201 cctcttaccc atcgaaaacg cacaaagttc ggcgctaaaa acgggcggtg ttcccagacg
     7261 gtatcgtaat cacggcgaac aacgcctgtt ccccctcccg aaaaaaagtg acgttccatc
     7321 caataagaag tataaaccac ttcttgttgg acgcccccga aaagggcttc gcttcgcttg
     7381 ctgcgcccct cgctccgccc ttttcgggg
//
LOCUS       DAOPXN010000111         7351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10089_length_7351_cov_4.650356, whole genome shotgun sequence.
ACCESSION   DAOPXN010000111 DAOPXN010000000
VERSION     DAOPXN010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10089_length_7351_cov_4.650356"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKM49_06895"
     CDS             complement(<1..462)
                     /locus_tag="PKM49_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HNY82577.1"
                     /translation="MKLEPSMREVLAYPKRELTVNFPVKMDDGSIKVFTGHRVQHNVA
                     RGPAKGGIRYHPDVTLDEVKALAFWMTWKGAVVGIPYGGGKGGVKVDPTLLSEGELER
                     LSRRYFSEIQVIIGEKTDIPAPDVNTSGKVMGWYMDTYSMNVGEPVLGIVTG"
     gene            824..1744
                     /locus_tag="PKM49_06900"
     CDS             824..1744
                     /locus_tag="PKM49_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82578.1"
                     /translation="MSEAILEVKNLKKYFQTVKAVDDISFNVNRGEVYTMLGPNGAGK
                     TTTLEIIEGIRKADEGEISFFGKRMKYVDRKTKEKIGVSLQTTAFIPHLKVKETIALF
                     ASFFQKSLPVDEVIGFVSLEEKKNAYAEKLSGGQRQRLAIACALINDPEFVFLDEPTT
                     GLDPQARRNIWDIIESLKLRGKTVFLTTHYMEEAQKLSDRVCILDHGKIVAIDTPEGH
                     IMKLGERNFIEFSCPMSPEDIAELNSWNGEPIKMEEEKVILPTKDLYGNLDRLMKWAN
                     QKSIILNDINIRRPNLEDVFLDKTGRRLRD"
     gene            1744..2916
                     /locus_tag="PKM49_06905"
     CDS             1744..2916
                     /locus_tag="PKM49_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82579.1"
                     /translation="MNLVFLKSIFLSSIRESEVMFWFFLFPVLLLTILCVIFTNMGTE
                     GIHFTAAVLDQGGNGVGSTIVRQIFDEISAEKEGHTSLFSILPVKTREEGERFLKEQR
                     VHIFIEIPEDFDPKFSQILFFSRFVGTKAQAPSIFIHQVKVRDASSLATEIMKQILTM
                     INGEAAKRMGVTLPSVTVEAKTVGAVQTFSMADFMFVGVVLMSFLSAGFFGLASDITT
                     YKSEHIYKRIFATPARTRDFFFGELITILCMCAISFTLLYLYASVVFHISLDIFKPGA
                     LIYVLLACVTSIAFGLFLGSVCKTPNRAAAFGNFLFFPMQFLGGLYFPVFNLSPWVDW
                     FIKINPVTYLAAGIRQEMGLLSSPFKPYLHYTVPLVWVSVLIGLAFLFFRRRGEEA"
     gene            2913..4037
                     /locus_tag="PKM49_06910"
     CDS             2913..4037
                     /locus_tag="PKM49_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82580.1"
                     /translation="MKTFWNMFLAFFYNRFRDFGSLFWNLAFPLMFMFIFGLAFGTGS
                     TGDSTTASSTERNWTHVGVYFSEGFSEDFKDRLADLVEDYGLKLFETPSEGELETGVI
                     RGSSGVRYGIAFTGDPKAMGLTAYLSAGNQNNAMYQSTVNSLAQDLRTKEANFREILN
                     VEVIEAEIYEKPVTTVGYILSGILSISITFSGLSALIISLGHFRKRKIIRRFLATPLK
                     GSTFLYADILNNYIFSFISLCILFLVSAGAFRIRFTIHLPYLALTFVSSLLFMMALGG
                     FFLVLLRSPKAAMNVSNLFSNVLIFFSGVYFPLEVLPEWLRSLAFYLPMTYVARSMRF
                     SLGQDFIPLSEFYTSNLIFLAIGLITIPLFGYFIFKREQE"
     gene            complement(4129..5304)
                     /locus_tag="PKM49_06915"
     CDS             complement(4129..5304)
                     /locus_tag="PKM49_06915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNY82581.1"
                     /translation="MDNFEYYNPTKIIFGKGTEAQVGAETAHCGRKILLHYGGGSIKK
                     SGLYDRVIRALKERGIKWVELGGAQPNPRLSLVRTGIEMCRKEEVDCILAVGGGSVID
                     SAKAIAMGVPYAGDVWDFFVGKAEVKAALPLGVVLTFPAAGSENSTGTVITNEDEWFK
                     RSAGSPLLRPKFAILNPELSYTLPAYQTACGVADMMAHIFERYFSKTEDVDLTDRLSE
                     ATLKTIIKHAGILMKEPAHYAARAEIMWAGTLAHNGLLDTGRASDWATHAMEHELSAL
                     YDIAHGAGLALLFPSWMRYVYPVFPAKFMQFFERVWDVEPDYVHPDRMIAEGIDRLKT
                     FYRSLGLPLSFQEAKLPVTDLDEMAEKCTRGGPIGSFKTLYKEDVLAIYRMASSLHA"
     gene            complement(5351..6313)
                     /gene="trxB"
                     /locus_tag="PKM49_06920"
     CDS             complement(5351..6313)
                     /gene="trxB"
                     /locus_tag="PKM49_06920"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNY82582.1"
                     /translation="MGHLDIGSIFKQESDVRDTYDCVIIGGGPGGLTAGIYAARGGLA
                     TLVLEKGVEGGTLNFTPVIENYPPYSSIVGMELAQKWSEHAERMGVTILHEGVVEVSF
                     FGENLTVKTDQGRTIAGKTIVISTGSSYRLLGLPSERRLAGKGVSYCALCDGALFKNK
                     KVAVVGGGSSAIDEALYLAQTAGSVTVIHRRDQLRAEKALADKAFQNPKIRFYWDTVV
                     VDIEGEQSVESLLLENVKTKERAREPFDGVFIYVGLDPSSGCFKGVLDMTEDGFIIAD
                     PHTLETSVKRVFAVGDVRQKEVRQIVTAAADGAIAASFIIKKYF"
     gene            complement(6322..6978)
                     /locus_tag="PKM49_06925"
     CDS             complement(6322..6978)
                     /locus_tag="PKM49_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY82583.1"
                     /translation="MPLLKEKDQQAVRERFAKELKNPVKINFFFSREDTSEETRITGE
                     LMDEIGGLSEGKLDVESIEGKDSERARRLRVDKMPALVIYGDGDERDNIRFFGLPAGH
                     EFATLLHTLITVSNDYHVELPDALVEKVRAIDKPVKIQVFVTTSCPHCPPAVVVSHYF
                     AMLNPMITSEMIEANQFDDLSRRYGVQSVPMTVINEGKQTFIGSRKPEELYNAIEKAL
                     "
BASE COUNT         1667 a         2010 c         1758 g         1916 t
ORIGIN      
        1 tcctgtgacg atgccgagaa ccggttctcc gacgttcatg ctgtaggtgt ccatatacca
       61 acccataacc ttaccgctcg tgttcacatc gggggcggga atatccgtct tttctccgat
      121 aatgacctga atctctgaga aatatcttct ggaaagtctc tccagttcgc cttcagagag
      181 aagagtggga tccaccttta caccgccttt tcccccgccg taaggaatcc ccacaaccgc
      241 ccctttccac gtcatccaga aggctaaggc tttcacttcg tccaaggtga catcggggtg
      301 ataccggata ccgcctttcg cgggtcccct ggcgacgttg tgctgaacgc ggtgaccggt
      361 aaagactttg atgctaccgt cgtccatctt aaccggaaag ttgacggtga gttctcgttt
      421 tggataggcc aagacctctc gcatactcgg ttctaacttc ataatgtcag ccgctttgta
      481 aaattgtttc aatgcgtttt caaataggct cacgtatctc cctcctttgg aattgctgtt
      541 ctcacacact ctattgtacc caaaaccaac gaaaaatcca aatccgagtt atccgtactt
      601 caagtctttc cactcgaaaa acccctagaa ggactcggtt tccttaccaa agtcgaggaa
      661 aacccgctgc tcgttccgat tacccgcgat tcagacctct ctcgtgctct attgactgtt
      721 gactaacttc atgatagtat cgctccgtga acaatcccgg atgcccacag atttctttct
      781 ttcgccaaac cgatgacaga cggggaacag ggggaggcgt aacgtgtccg aagcgatact
      841 ggaagtgaaa aacctgaaaa aatatttcca aactgtcaaa gcggtggacg acatctcttt
      901 caatgtgaat cgcggcgagg tatacaccat gttaggcccg aatggcgctg gaaagaccac
      961 gaccttagaa atcatcgagg ggattcgaaa agccgatgag ggagagatca gcttctttgg
     1021 aaaaaggatg aagtatgtcg atcgtaaaac gaaagaaaag atcggcgttt cactgcaaac
     1081 aactgcattc atcccccatt tgaaggtcaa agagacgatc gcgctattcg cgtccttctt
     1141 tcaaaaatct ctacccgttg atgaagtgat cgggttcgtc tccctggagg agaagaaaaa
     1201 cgcttacgcc gaaaagctat ccggagggca aagacagcgg ttagccatcg cctgcgctct
     1261 catcaacgac cccgagttcg tatttttgga tgaaccgacc accggtttgg accctcaagc
     1321 tcggcgcaac atctgggaca tcatcgaaag cttgaagtta cgggggaaga cagtcttcct
     1381 cacgacccac tatatggaag aggctcagaa actcagcgat cgcgtgtgta tcctggatca
     1441 cggcaagatc gtggcaatcg atacccccga agggcatatt atgaagctgg gagaacggaa
     1501 cttcatcgaa ttttcctgtc caatgagccc ggaggacatc gccgagctca attcttggaa
     1561 tggagaaccg atcaaaatgg aagaagagaa ggtcatttta ccaacaaaag acctttacgg
     1621 aaatctagac aggttaatga agtgggcgaa ccaaaaatcg atcatcctga acgacatcaa
     1681 catacgccgc ccaaatttgg aagacgtctt cttggacaaa acgggaagga ggttgcgaga
     1741 ctaatgaatc tcgtattcct caaatccata ttcctgtcga gtatccggga atctgaggtt
     1801 atgttctggt tttttttatt cccggtattg ctcctgacca tactgtgtgt catattcacg
     1861 aatatgggga cggaaggcat ccatttcact gccgcggttc tagaccaagg gggaaacggc
     1921 gtaggatcga caatcgttcg ccagatcttc gatgagatca gtgccgagaa agaaggacat
     1981 acctcgcttt tttcgatact ccctgtcaag acgcgtgagg agggagaacg gttcctcaaa
     2041 gagcagcggg tccacatttt catagaaatc cccgaggact tcgatcccaa gttcagccag
     2101 attttgttct tctcgcgttt cgtcggcact aaggcgcaag cgcccagcat cttcattcat
     2161 caagtgaagg ttagggatgc ctcctcgctt gcgaccgaaa ttatgaaaca gattcttacg
     2221 atgatcaacg gggaagcggc gaagcggatg ggggtcaccc tgcctagcgt gacggtggaa
     2281 gcgaaaacgg tcggcgctgt ccaaaccttt tctatggctg acttcatgtt cgtgggagtc
     2341 gtcttgatgt cctttttaag cgcgggcttc tttggtctgg cttcagacat caccacctat
     2401 aaaagtgagc atatctacaa acgtatcttc gccacgcccg cgagaacgag agattttttt
     2461 ttcggagagc tgattactat cctctgtatg tgcgccatct cttttacgct actctacctg
     2521 tacgcaagcg tcgttttcca catatccctc gacatcttca aaccgggcgc cctcatttac
     2581 gtgctgctgg cttgtgtcac gagcatcgca ttcggtctat tcttgggaag cgtctgtaaa
     2641 acacccaata gagccgccgc ttttggtaat ttcctgtttt tcccgatgca attcttggga
     2701 ggtctttact tccccgtttt caacctttcg ccctgggttg attggttcat caaaattaac
     2761 ccggtgactt atctcgccgc gggaatccgt caagagatgg gcctactctc ctctcctttt
     2821 aagccatacc tgcattatac cgtccccctg gtgtgggtga gtgtgctcat aggtcttgcg
     2881 tttctctttt tccgaaggag aggtgaagag gcgtgaaaac cttctggaat atgttcctgg
     2941 cctttttcta taatcgcttt cgagatttcg gtagcctctt ctggaatctg gccttccccc
     3001 tgatgtttat gttcatcttt gggctcgctt tcgggaccgg ctccaccgga gattcgacaa
     3061 cagcctcctc gactgagagg aactggactc acgtcggggt ctacttttct gaagggtttt
     3121 ccgaagactt caaagaccgc ctggccgatc tcgtggaaga ctacgggctg aagctttttg
     3181 aaaccccatc agaaggagaa ttggaaacag gggtgatccg cggaagctcc ggagtgcgtt
     3241 acggcatcgc ttttaccgga gacccgaaag cgatggggct aaccgcctat ctgagcgcgg
     3301 gcaatcaaaa caatgcgatg tatcagtcca cggtcaattc cctggcacag gacttacgaa
     3361 ctaaagaagc aaatttccga gagattttaa acgtggaagt catagaggct gaaatctatg
     3421 aaaagccggt gaccacggtc ggctacatcc tgtccggtat cctatcgatc tctataacct
     3481 tttccggact ttccgcgttg atcattagct tagggcactt caggaaacgc aagatcatac
     3541 gtcgatttct agcgacgccc ttgaaaggct cgaccttctt atacgcggac atcctcaata
     3601 actacatctt ttccttcatc tcgttatgta tcttgtttct cgtctcagcc ggggccttcc
     3661 gcatcaggtt cacgatccat ctgccctatt tggcgctcac cttcgtgtct tcactgttgt
     3721 ttatgatggc gttaggcggg ttttttctcg ttcttttacg tagcccgaag gcggcgatga
     3781 acgtttcaaa cctgttttcg aacgtcctca tcttcttttc cggggtttat ttcccgttgg
     3841 aagtattgcc cgagtggttg cggtccctcg ccttctacct cccgatgact tacgtggcca
     3901 gatcgatgcg gttttctttg ggccaggatt tcatacctct atcggagttc tatacgtcga
     3961 acctcatctt tctggcgatt ggcttgatca ccatcccgct tttcggttac tttattttca
     4021 agagggagca agaataggga taaaaagctg gacgtacgcg cgacgcacat tttcctcttc
     4081 cccggtttcg tgaacgccct atgagtattc tttgaaagaa ctcgcggttt atgcgtgaag
     4141 gctgctcgcc atgcgataga tcgctaacac gtcctctttg tacaacgttt tgaagcttcc
     4201 gatcggtcct ccccgggtgc atttttcggc catctcatcg aggtccgtga ccggcaactt
     4261 ggcctcttgg aagctgagtg ggagccccaa cgaccggtag aaagtcttca gccgatcgat
     4321 cccttctgcg atcatacggt ccggatgcac gtagtctggt tcgacgtccc aaaccctctc
     4381 gaaaaactgc ataaatttcg cgggaaatac cgggtaaaca taccgcatcc acgagggaaa
     4441 gagaagggcc aaaccggcgc catgggcgat atcgtacaag gcgctcagtt cgtgttccat
     4501 cgcgtgggtg gcccaatccg aggctctgcc cgtgtctagc aaaccgttgt gcgcaagggt
     4561 gccggcccac atgatctccg cgcgcgcggc gtaatgcgcg ggttccttca tcaggatgcc
     4621 ggcgtgcttg ataatcgttt tcagtgtcgc ttcactcagc cggtcggtga gatcgacatc
     4681 ctcagttttg gaaaagtacc tctcgaagat gtgcgccatc atatccgcga ctccgcacgc
     4741 cgtctgatag gcgggcaagg tgtaagagag ttcgggattg agaatcgcga actttggtcg
     4801 aaggaggggg ctgcccgcag accgtttgaa ccattcgtcc tcgtttgtga tcacggtacc
     4861 cgtgctattc tcgctacccg ctgccgggaa ggtcaatacg acacccaatg gcaacgccgc
     4921 tttcacttcg gcttttccca cgaaaaagtc ccagacgtcg cccgcgtagg gaacccccat
     4981 agcgatcgcc ttcgcggaat cgatcacgct tccgcctccg accgcgagga tacaatctac
     5041 ctcctctttt cggcacattt ctattcccgt tcttaccaga gagagacgcg gattgggttg
     5101 tgcgcctccc aattccaccc acttgatacc tcgctctttt aacgcgcgaa tgacacgatc
     5161 gtatagaccg ctttttttaa tgctgccacc gccgtagtgt agcaagattt ttcggccgca
     5221 atgagccgtt tctgctccga cttgcgcttc tgtccctttt ccaaaaatga tcttggtcgg
     5281 attgtaatat tcgaaattgt ccatcccact cctcctgcac ttatgagatg tccacgtgag
     5341 gacatcacat ttagaaatat ttcttgatga taaatgaagc cgcaatcgca ccgtccgccg
     5401 ccgccgtcac gatctgacgg acctctttct gtcggacatc tcccaccgcg aagactcttt
     5461 ttaccgaagt ttctaaggta tgcggatccg cgatgatgaa cccgtcttcg gtcatatcca
     5521 aaaccccttt gaagcagccg ctgctcgggt ccagtccaac atagatgaaa accccgtcaa
     5581 agggttcccg ggcgcgctct tttgttttaa cgttttccaa aagcaacgac tctacgctct
     5641 gctcaccttc gatatcgacc acgaccgtgt cccaataaaa ccgtatcttg gggttctgaa
     5701 aggctttatc cgctaaggct ttctctgcgc gtaactggtc acgtctatga atgactgtca
     5761 cagagcccgc tgtttgggcc agatatagcg cctcgtcgat cgcggagctc ccgccgccaa
     5821 cgaccgcaac ctttttgttc ttaaataagg ctccatcgca tagcgcgcag taggaaaccc
     5881 cttttccggc gagccgacgc tctgaaggca gccccagcag gcggtaggaa gaaccggtcg
     5941 aaatcacgat cgtctttccg gcgatggttc gcccttgatc tgttttcacc gtgaggtttt
     6001 ctccaaaaaa cgatacctcg acgactccct catgtaaaat cgtaacgccc atccgttccg
     6061 cgtgctcgct ccatttttgc gctaattcca tcccaacgat cgaggagtat ggggggtagt
     6121 tctcgatcac cggggtgaag ttcaaagtgc ccccttccac ccctttctcc agcacgagcg
     6181 tggctaagcc cccacgcgcg gcgtatatac cggccgtgag tcccccaggc cctccgccga
     6241 tgatcacaca gtcataggtg tccctcacgt cgctctcttg cttaaagatc gaccctatat
     6301 ccaagtgccc cataattcct cctaaagcgc tttttctatc gcattgtaga gctcttcggg
     6361 ttttcgtgat ccgatgaatg tttgctttcc ttcgttgatc acggtcatag gcaccgattg
     6421 aacgccgtat cgcctggata ggtcatcgaa ttgattcgct tcgatcatct ccgaagtgat
     6481 catcgggttg agcatcgcga aatagtgtga gaccacaacc gccggcggac aatgagggca
     6541 actcgtcgtc acgaagactt gtattttcac gggtttatct atcgcgcgta ctttctcgac
     6601 cagcgcatcc ggcagctcca cgtgatagtc gttcgatact gtgattagtg tgtgcaagag
     6661 tgtggcgaat tcgtggcccg cgggtaggcc aaaaaaccgg atgttgtctc gctcgtcccc
     6721 gtcaccgtat atgacgagcg ccggcatttt gtctacgcgc aaacgccgcg cccgctcgct
     6781 gtccttcccc tctatggatt ccacgtcgag cttgccttcg gataaccctc ctatctcatc
     6841 catcaattcg cctgtgatcc tcgtctcttc actcgtgtct tccctcgaaa aaaagaaatt
     6901 gatcttcacc gggtttttga gttctttggc gaacctttcc ctgaccgctt gctgatcctt
     6961 ctctttcaat aatggcatag tattccctcc tcgattatat accctatagg ggtatatgta
     7021 agacgaaatc gtgcagcgct ttctttttgc aaagcgttat attataatac gaaaaaggca
     7081 aaacgagact ttgagtgtgg atatactatc gaggtgcggc aaaaaagatt agaggaggaa
     7141 gctcgccttt tactcttttt tccgctacga tgagccgcgg ggttatcgcg gggtaagtgg
     7201 tttttacgac cccgcgatcc gccctacgcc cttttcaaat gcgtatcgcg ccgtcgcgaa
     7261 aagaaggggc gcttacgccg cgatgctctc gtgggttttc gcggggtcgt aaaaaccact
     7321 taccccgcga taacccacga gagcatcgcg g
//
LOCUS       DAOPXN010000112         7302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10183_length_7302_cov_5.561474, whole genome shotgun sequence.
ACCESSION   DAOPXN010000112 DAOPXN010000000
VERSION     DAOPXN010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_7302_cov_5.561474"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1229
                     /locus_tag="PKM49_06930"
     CDS             171..1229
                     /locus_tag="PKM49_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY82584.1"
                     /translation="MSIEQTKVSIVIPVYRAENTLERCLASVFAQTHSNLEVILIDDG
                     SPDRSPEICDSYAQKDERIRVIHQSNTGVGAARNAGMSVMSGAYLFFLDADDYIVPET
                     ITVLVNRAEKTGADITIGNLRESGGRGLVRESPPYSRESILSEQQAIAQVRYELFYNP
                     GYGVTAWNKLYRAGFLEEHGIAFENRRLLCEDRVFNTRCFIHRPRIELVNRYTYVYCI
                     DASTITRSKIDNPVEKSLSAVEAMKDALDANQSADENEDLFTWFVFGQINQCARNFAI
                     HSERPFKDIKAFLQQYRENDLVRRCLEEIGSGKHMEGISRTSWKRYARGLSRLLSRGA
                     YTKASMILWLRFKVIEHR"
     gene            1853..2620
                     /locus_tag="PKM49_06935"
     CDS             1853..2620
                     /locus_tag="PKM49_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY82585.1"
                     /translation="MGYIKGSEASAAVDGGNSLKHMRLFLKHLREERLAVPTFTFLTH
                     WHWDHIFGLGALDNWIVASEETKKYMLPLQDQDLEQEIRESDYQIKEEFTDEFDVALR
                     MPSIVYKERCVFDLGNLTVLAQWVESDHTADCSVFFVPEEKVLFIGDCLYCGADHGQY
                     YLDRLRYLTLLKTLLRFPAELYLDSHRKPITRERLRADLEKVSRIAEVSVQYPDVGEA
                     LRFLEAENRFDVDDDVAFYLKAFENGEKRNRGFTGNP"
     gene            2654..3418
                     /locus_tag="PKM49_06940"
     CDS             2654..3418
                     /locus_tag="PKM49_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82586.1"
                     /translation="MPKNNMIFWTLFCAFCALLGLGLLATGILINEMETMWMVVLGFF
                     LMVTFVICTLLFWKQARLLRQMFEGKRLLAHWVYSKEKAVQHAQGEKVRRAKANLLLW
                     LIIAGFVVFFSVLFVVFGDMDEDGIRFFLSIMGGVLAITGLAAWLAPILAERKIKRSV
                     PEVFIGEFAVWAFGEFDIWNSAMTQFRSVSTGQITEEDGSPLAGEVSRKIEIVYEQGN
                     RYGYQERKVLIPIPVGKEQEAEEVVRRILDANSSSH"
     gene            3558..4364
                     /locus_tag="PKM49_06945"
     CDS             3558..4364
                     /locus_tag="PKM49_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HNY82587.1"
                     /translation="MRFWQVDSFTAQPFKGNPAGVCVTETPLPADLMQGIAAEMNLSE
                     TAFAVPLDRQGGYSLRWFTPEKEVPLCGHATLATAAILYEYFKEKGKELRFQTLSGVL
                     KTRYREGAIEMDFPFYHPQPISLPEETLRSLALPAIRDSRYAAELRYWLFALESEEEV
                     RRYKPDFSHWKSLVFPCEVSGIILTAESGGEHDFVSRFFAPWLGVDEDPVTGSSHTVL
                     GPYWQEKTGKRRLRAYQCSKRGGEMLLTVQEERVRIEGRYALVLEGVLKA"
     gene            complement(4371..5480)
                     /gene="hflX"
                     /locus_tag="PKM49_06950"
     CDS             complement(4371..5480)
                     /gene="hflX"
                     /locus_tag="PKM49_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNY82588.1"
                     /translation="MEKALLVGVWTKDKEEWEFDDSLHELESLLKTAGGEKAFELRQK
                     VHELHSATYIGKGKLAELKQAVAELNPDLVIFDNELTPVQARNIEKEIDCHVIDRTQL
                     ILDIFALHAKTKEGRLQVELAQAVYRLPRLVGMGKEMSRLGGGIGTRGPGETKLEAKR
                     RVLVLKIRELKKELKALKKQRGIQRKTRIDRHIPIVSLVGYTNSGKSTLLRALSKDQD
                     IYVADALFATLSPVTRKVFMPSRSTVVMNDTVGFIQGIPHTIIEAFMATFEEIRFSQL
                     LLLVVDASDPRYEAKINASRRVLDKIEASDIPVLLVFNKCDLLDEAKRELIRTQYPES
                     VLVSALKKTDLPNLLDAIEAGLRRETAEKTNRQTF"
     gene            complement(5495..6775)
                     /locus_tag="PKM49_06955"
     CDS             complement(5495..6775)
                     /locus_tag="PKM49_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82589.1"
                     /translation="MKKLLILILLLTSVAINASHALIHWETTYGGKEGDSVRNAALLQ
                     DGSVLLTGFGGSTELFTGRNRKVDIFVTLMDSKGQFLRSGNFGGMGEDRGYTAIQTAS
                     GDFLIAGASASTEGGRSDPRGLWDGWVIRTDPDFNIRWEKSYGGKDNDSIREIIEAKD
                     GGYIFCGYTQSPNLENFHGGKDFWIVRLDEAGAVLWEKVYGGSGFDMAYSIAQTSDYG
                     FVVAGYTFSSDGDLKGKTHRGNGDWWIIKLNRYGDIEWHETYGGSGWDEPRKIAVTSD
                     KGYIITGVTGSNDGHISGNNGRWDVWLLKLNRSGKVEWSKTFGGSDHDKAFSLTIDRR
                     GNYFLAGYTTSSDGDVSQHHGGMDAWVLRVSASGSLMWEHTFGTERDECAESIIQMKD
                     GTYLFAGGVFKGKPEIAGDSGRLYEDYWIVNFQP"
     gene            complement(6977..>7302)
                     /locus_tag="PKM49_06960"
     CDS             complement(6977..>7302)
                     /locus_tag="PKM49_06960"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82590.1"
                     /translation="EKMQTIADMLRQEGMEKGIMKGREEGREEGREEGREEGREEGLE
                     KGILKGKEEGREELLWKLIAKKFPKASQKYFERLKSLTIEQLDFLGLELIDMKNEEEL
                     KKHLM"
BASE COUNT         1619 a         1962 c         1937 g         1784 t
ORIGIN      
        1 cggatgcgga tccgataccg agaggtgtat gatcaggtga tgggcgaaaa ttgaccttct
       61 ctggctcgat tccgcccgtt caagaaaagg acattacgga tagcgccaaa gtcgtttatt
      121 ttgataacga tctgtgtcag accgcccaca ccgctgagtg gagaaatcag atgagcatag
      181 aacagaccaa ggtcagcatc gtcatacccg tttaccgggc agagaacacg ttagagcgct
      241 gcctggccag cgtgttcgcg caaacccact cgaatctcga ggtgatactg attgacgacg
      301 gctctcccga ccgtagcccg gagatttgcg actcttacgc ccagaaagac gaacgtatcc
      361 gcgtgatcca tcagtcgaat accggagtcg gcgctgcgcg aaacgcgggg atgtctgtga
      421 tgtcgggcgc ctacctgttt ttcttagacg cggatgacta catcgttccc gaaacgatca
      481 cagtgttagt caaccgggcc gagaaaaccg gtgcggatat cacaataggc aacctccggg
      541 agtcgggcgg gcggggccta gtccgcgaga gtccccctta ttcccgagag agtatcctct
      601 cggagcagca agccatcgcg caagtacggt atgagctatt ctacaatccc ggttacgggg
      661 tgacggcctg gaacaagttg tatcgtgcgg gcttcctcga ggaacacggg atagccttcg
      721 agaatcgtcg actcctgtgc gaagaccgcg tattcaatac cagatgcttc atacatcggc
      781 cgcgtatcga gttggtcaac cgatacacct acgtctattg catcgatgcg tcgacgatta
      841 cgagatcgaa gatcgataat ccggtagaga aaagtctgag cgcggtagag gcgatgaaag
      901 acgcgcttga cgcgaatcag agcgcggatg agaacgaaga ccttttcact tggttcgtgt
      961 tcgggcaaat caaccaatgc gcccgaaatt tcgctataca tagcgaacgt ccattcaagg
     1021 atataaaggc tttcctccag cagtatcgag agaacgatct cgtcaggcgc tgtcttgagg
     1081 agatcggttc gggaaagcat atggaaggga tttctcgaac ttcgtggaaa cggtatgcga
     1141 ggggtctttc ccggttgctg agccgaggcg cgtacacaaa agcttctatg attttgtggt
     1201 tgcgtttcaa ggttatagaa caccggtagc gctaaggcgc gcctttctgg gagcgccttt
     1261 ggaacgtcct gagaacgcgg ggctttagtc cggcttgtct tcggtatttc cgctaagagc
     1321 gcttttcgta ggagtgcccg atctactttt cgcctacctc gtatttcgtc ttctcacgcg
     1381 ccctccgtgt gaaccctgtt tttcaggaca tgccaacata aaagttagcg ttcccagagc
     1441 gttcccgagc gcggcatcgc ggcgtaagcg ccccttcttc tcgtgacggc gcgatgccct
     1501 ttttagaagg acataaggac ggttcgcagg gcccaaaaaa ccacttaccc cgcgataacc
     1561 tcgcagaccc atcgaggcgt aaaaagggca aaaggccgat ctcctcctct ttctgttttt
     1621 tgttgcacct cgatacgata cccacacccc ttgggagtat ttgcccaaaa acgtgttatt
     1681 atagacagta gatcgtcgaa actatcgtgc gactcgacca cttcctcgcg gaaaccgccg
     1741 ggataggggc ggtcgagaga gcgcgcgaaa ggggtggtgt atgaacgaat gggttcgctt
     1801 aacacagcgg gttgcttggt taacaccttc tgaggagacg gatcgacccg gtttgggcta
     1861 tattaagggg tccgaagctt cggccgcagt ggatggtgga aattccctca agcatatgcg
     1921 gcttttctta aaacacctgc gtgaagagag gctagccgtt ccgaccttta ccttcctcac
     1981 gcattggcat tgggaccaca tctttggcct aggcgccttg gacaactgga tcgtagcatc
     2041 cgaagagaca aaaaagtata tgctaccctt acaagaccaa gatttggaac aggagatccg
     2101 ggaaagcgac tatcagatca aagaggaatt taccgacgaa ttcgacgtgg cgttgcgtat
     2161 gccttcgatc gtctacaaag agcggtgtgt cttcgacctc gggaacttaa ccgtactcgc
     2221 gcagtgggta gagtcggatc atacggcgga ttgttccgta ttcttcgtcc cagaagaaaa
     2281 agtcctcttc atcggagatt gcctttattg cggggcggat cacgggcaat actacctgga
     2341 tcggttgagg tatttgacat tgctgaagac gctgctgcgc tttccggcgg aactctacct
     2401 agactcgcat cgcaaaccca tcaccaggga gcgtttaagg gccgatctcg aaaaggtctc
     2461 tcggatcgcg gaagtgtcgg tccaataccc cgatgtgggg gaagccctcc gctttttgga
     2521 agcggagaac cgtttcgacg tggacgacga tgtcgccttc tatttgaaag ccttcgagaa
     2581 cggagaaaaa cgaaaccgag gttttaccgg taatccgtga gaaagaagtc aaatcgggag
     2641 cgagggggta aagatgccga agaataatat gatcttttgg acgctttttt gtgctttttg
     2701 cgcgcttctc ggccttggat tgctggccac tgggatactc attaatgaaa tggaaacgat
     2761 gtggatggtc gtgctcggat ttttcttgat ggtgactttc gtgatttgca ccctgctctt
     2821 ctggaagcaa gcgcgcttac tccgtcagat gttcgaagga aagcggcttt tagcgcattg
     2881 ggtgtactcg aaagaaaaag cggtgcagca cgcgcaagga gaaaaggtgc gccgggcgaa
     2941 ggcaaacctt cttttgtggc tcatcatcgc cggcttcgtc gtctttttca gcgtgctgtt
     3001 tgtcgtgttc ggtgatatgg acgaagatgg gattcgcttt tttttgtcga ttatgggagg
     3061 ggtgctcgcc attactggct tagctgcctg gctcgcgccg atactggctg aacgaaagat
     3121 caagcgctcc gttcctgagg ttttcatcgg cgaattcgcc gtctgggcat ttggggagtt
     3181 tgacatctgg aattcggcca tgacacagtt caggagcgtg tctaccggtc agatcaccga
     3241 agaggatggg agtccgctgg caggagaggt ttctagaaag atcgagatcg tttatgaaca
     3301 agggaatcgg tatgggtatc aagagcggaa ggtgctcatt cccatccccg tcgggaaaga
     3361 gcaagaggcc gaagaggtcg tccgtcgtat cttggacgca aattcctctt ctcattaacg
     3421 ttgtactcca ggctagagcg ctcgtcaacc caagccaggg cgtgtccgtc gttcacgggg
     3481 accctttgag cagccctccc gaacgacgcg cgagcacgga tgacgtgtga taacggaagg
     3541 agagagacgc tttggacgtg aggttttggc aagtggattc ttttacggcc cagcccttca
     3601 aggggaatcc ggccggggtt tgcgtgaccg agaccccttt acccgcggat ttaatgcaag
     3661 ggatagcggc ggagatgaac ctctccgaaa cggccttcgc cgtcccgctc gaccgacagg
     3721 ggggctattc gctgcgatgg ttcactcccg aaaaagaggt accgctctgc ggacacgcca
     3781 cgttggcgac cgcggccatc ctttacgagt atttcaaaga gaagggaaag gagttgcgct
     3841 ttcaaaccct gagcggagtt ctaaaaacga gataccgaga gggggcgatc gagatggact
     3901 tcccctttta ccacccgcag cccataagtc ttcctgagga aacgctcaga tcgctagcgc
     3961 tacccgctat ccgagactct cgttacgcgg ccgaactccg ttactggctc ttcgcactcg
     4021 aaagcgagga agaggtcaga cgctacaaac cggatttctc acattggaag agtctggtgt
     4081 tcccctgcga ggtcagcgga atcatcctaa cagccgagag cggcggcgag cacgatttcg
     4141 tttcgcgttt tttcgcccct tggcttggtg tcgacgagga ccccgtgacc ggctcctctc
     4201 atacagtttt gggtccgtac tggcaagaaa aaaccggaaa acggcggttg cgtgcctacc
     4261 aatgctcgaa gcgcggcggg gagatgcttc tcacggtaca ggaggaacgc gtgcgtatag
     4321 agggccggta cgcccttgtc ttggaagggg tgttgaaggc gtaaaggggc ttaaaatgtc
     4381 tgccggttgg tcttttcggc cgtctctcga cgcaacccgg cttctatggc gtcgagcaaa
     4441 ttcggtagat ccgttttctt caaagccgaa accaaaaccg actccggata ttgcgtccgg
     4501 atcaattctc gtttcgcttc gtccagtaaa tcacatttgt tgaacaccaa caggacgggg
     4561 atgtccgagg cctcgatctt gtccagcacc cggcgactgg cgttgatttt cgcctcatat
     4621 cggggatcgc tggcgtccac caccagcagc agcaattgac tgaaccggat ctcttcgaag
     4681 gtagccatga acgcttcgat gatcgtgtga gggattcctt ggatgaatcc gacggtgtcg
     4741 ttcatcacga cggtggatcg agagggcatg aagacctttc tcgtcacagg gctgagtgtg
     4801 gcgaaaaggg cgtccgccac atagatgtct tgatccttcg ataaggctcg cagcaaggtg
     4861 gatttaccgc tgttcgtgta gccaaccagg gagacgatcg gtatatgccg gtcgatgcgc
     4921 gtctttcgtt gtatgcctct ttgttttttc aaggccttca attctttctt gagctctcgg
     4981 atcttgagca cgaggacgcg ccgcttggct tccaatttcg tctctcccgg cccgcgggtt
     5041 ccgataccgc cgcctaaccg agacatctct ttacccatcc ccaccaggcg cgggagacgg
     5101 tagaccgcct gagccagctc cacttgcaag cgcccttctt tcgttttagc gtgcaaggcg
     5161 aaaatatcga ggatgagctg ggtgcggtcg atgacgtgac agtcgatctc cttttcgatg
     5221 tttcgagcct gcaccggggt caactcgttg tcgaagatga cgagatcagg gttcagttcc
     5281 gctaccgctt gtttgagctc cgccagtttc ccctttccga tgtatgtggc cgaatgcaac
     5341 tcgtgaacct tttgtctgag ctcgaaggcc ttctctccgc cggccgtctt cagcagcgat
     5401 tctaattcgt gcagcgaatc gtcgaactcc cactcttcct tgtctttcgt ccatacccca
     5461 acgagaagcg ctttttccat tatgtctttt ccacttaggg ttggaagttt acgatccaat
     5521 agtcctcgta caagcgccca ctatcgccgg caatctcggg tttgcctttg aaaacgcccc
     5581 ctgcaaacag gtaggtaccg tctttcattt ggatgatgct ttccgcgcac tcgtctcttt
     5641 ctgtcccaaa ggtatgctcc cacatcaagg aacccgaagc gctcacgcgg agcacccacg
     5701 cgtccatccc cccatggtgt tgactcacgt cgccgtcgga agaggtggtg tatccggcga
     5761 ggaaatagtt gccccgtcga tcgatggtca gtgagaaggc cttgtcgtgg tcgctcccgc
     5821 cgaaggtttt cgaccactcg acctttccgg accgattcag cttcagcaac cagacatccc
     5881 aacgcccatt gttcccgcta atatggccgt cattggatcc ggtaacgccg gtgatgatat
     5941 accccttatc ggaggtgacg gctatttttc tgggctcatc ccaaccagag ccgccatagg
     6001 tttcgtgcca ttcgatatcg ccgtaacgat tcaacttgat gatccaccaa tccccgtttc
     6061 cgcgatgtgt cttgcccttc aggtcaccgt cggaagagaa ggtgtatccg gcgacgacga
     6121 aaccgtagtc ggaggtttga gcgatggaat acgccatatc gaaaccggag cccccgtaga
     6181 ccttttccca aaggaccgct cccgcttcgt ccaaacgaac gatccagaag tcttttccac
     6241 cgtgaaagtt ttctaaattc ggagactgcg tgtatccgca gaagatatac ccgccgtctt
     6301 tcgcttcgat gatctctcgg atggaatcgt tgtcctttcc cccgtaactc ttttcccacc
     6361 ggatgttgaa atcagggtca gtccggatga cccaaccgtc ccacaacccc ctggggtcgg
     6421 aacggcctcc ttcggtggaa gccgaagcgc ccgcgattaa aaaatccccc gaggcggttt
     6481 gaatcgccgt atacccgcga tcctctccca tacctccgaa gttcccggag cgcaagaact
     6541 gccccttcga atccattagc gtcacgaata tatcgacctt ccgattcctt cctgtaaata
     6601 gctcagtcga accgccaaat ccggtcaaca acacgcttcc atcctgtaga agggcggcgt
     6661 ttcgaacgct gtcaccctct ttaccaccgt aagtcgtttc ccagtgaatc aacgcgtggc
     6721 tggcgttgat ggcgacactc gtcaacaata gtataaggat caatagtttt ttcaagaaag
     6781 ccctcctctc atatgacacc ggtaggggta ttctatcaca cacgatcgcg cttctaacca
     6841 tctcgtgtgg gtataatatc cgggtgtgat aagaaaaaaa gacaaaaaca aggagggaaa
     6901 aaaggccccc gaaggagcca ccataacttc gcacacacgg cccatcaaat cgggttcaag
     6961 ccctttgtcg gactctctac attaaatgct tctttaactc ttcctcgttt ttcatatcga
     7021 ttagctcaag tcccaagaag tcaagttgct ctattgtaag ggacttcagc ctttcgaaat
     7081 acttttgcga ggccttaggg aattttttgg ctattaattt ccaaaggagt tcttctcggc
     7141 cttcttcttt gccttttaga atcccctttt ctaagccttc ttctctgcct tcttctctgc
     7201 cttcttctct gccttcttct ctgccttctt ctctgccttt cataattcct ttttccattc
     7261 cttcctgtct gagcatatcc gcgattgttt gcatcttttc gc
//
LOCUS       DAOPXN010000113         7086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10591_length_7086_cov_5.015361, whole genome shotgun sequence.
ACCESSION   DAOPXN010000113 DAOPXN010000000
VERSION     DAOPXN010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_7086_cov_5.015361"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PKM49_06965"
     CDS             <1..518
                     /locus_tag="PKM49_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82591.1"
                     /translation="ERIESGEVKLTVYLFRAVVAPDKEREMRWIIEAMQALKEVSGRR
                     DIEDLIDLGFRYLLSLNLRIELEELVEIARRFSEERGEQIMTLAERLIDKGMQKGREN
                     GLKEGLKEGLKEGLKEGLEKGLERGLEKGREEEKYIIAKRLILSGMDLSEVSVITGLS
                     LEDLQKIDRLS"
     gene            1079..1780
                     /gene="pseF"
                     /locus_tag="PKM49_06970"
     CDS             1079..1780
                     /gene="pseF"
                     /locus_tag="PKM49_06970"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HNY82592.1"
                     /translation="MITRNVAIIPARGGSKRIPKKNILPFLGKPIIAYSIETALESGL
                     FDEVMVSTDDSAIADIAREHGAVIPFMRDQNTANDRAVIAEVCSEVLQKYQLQGKEFD
                     NVCMIMATAPFVTAQRLKEAYSKLSEGNYDCIMTVTEYSFPIQRSFKLENGLLLMRWP
                     ENIHVRSQDLEKTYHDAGQFCFFKVNSFLKQRILFMERTGMILLDAIEVQDIDTPIDW
                     ELAELKYQFLRSKKQ"
     gene            complement(1808..3817)
                     /locus_tag="PKM49_06975"
     CDS             complement(1808..3817)
                     /locus_tag="PKM49_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNY82593.1"
                     /translation="MSRYKKIKNPTAKKDGPVVSVTPVKVAKGFFESNLNFLRQRDPA
                     LAQRVETHALSGQYRVMSTGNPPRLNLLLPDGKSFFYAASDPEQDTRNELEKLNLKNT
                     QAALFLGIGLGYELDLFLRDHVKKAGTTHLIIIEKEMDIFYMTLKTLDYASIAKAFSL
                     HFAVGEKEDDLFLRLQEKFAADQKLLVLLKAMRPIFHSSSMRLGKEYYLRALRAFREA
                     GAYQLNNFGNSIEDSLIGVRNMLENINEIIYNPGINLLYNQFKGKPGIVVSTGPSLNK
                     NKHLLKGLDDKAVIVSPDASLRILVDMGVKPHLITSLERVLELAKLVDGFTVEQVENV
                     YFAACPVVFNEAYQTYPGPRIIVYRNFDHFKWLRVDRGILDIKQSSGNMAFKILDALG
                     CDPIILIGQDLAYSREGTTHASGSTFGEKQTTHNVPTFNVMGNDGQPILTNTIWNGFR
                     KSYEIDVAEYKGQCINATEGGAFIKGTTVMTFQEAIDKYISEPFSPLDKIKTLVSGFS
                     KEEADRDAEKIGGLVDRTLDDMQHVYDTCKAGLDWLLSIDEVLEEIKKGLLDERRAKE
                     KINLFQSTLTGYKVKVHERKENFQLFFMHIIQSFYLNFDIERNAVPNQFENEMDAAVE
                     SVRIHQKWFSTIGDTAMICVDLLKHTQETIHSKIHGFREQRVQAL"
     gene            complement(4217..4717)
                     /locus_tag="PKM49_06980"
     CDS             complement(4217..4717)
                     /locus_tag="PKM49_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY82594.1"
                     /translation="MPREVVKRARLILNESDKNQNKQERLRKYLQIIEDYADKGYGSC
                     LLKDEGNAIIFRDTLLSQDATIYSWVIMPNHVHFLMKRSPAVTLSEYIKRLKQVSTYR
                     INARLGKQGPIWLREYYDRYMRTETHFERAKRYIKNNPIKAGLVKDISDWPWYGELST
                     TGNVEL"
     gene            complement(4895..>5140)
                     /locus_tag="PKM49_06985"
     CDS             complement(4895..>5140)
                     /locus_tag="PKM49_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82595.1"
                     /translation="APSFCDGAIPLRTLGTPTYKLACLMVFGKASRRKRSFFLRRRDT
                     LENAGNANFQVGMFSALWNEKDTTHATPKSNIPKASP"
     assembly_gap    5141..5240
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5334..6785)
                     /locus_tag="PKM49_06990"
     CDS             complement(5334..6785)
                     /locus_tag="PKM49_06990"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82596.1"
                     /translation="MKETATIVFPDGRKAYEGELEDGFLNGTGTLYYRDGQTIHYRGG
                     WRQNLPDGDGNLFFPDGSLWYVGTWKAGLREGRGKEYDAAGAITYDGGWKANHPEGQG
                     IKYHPNGQPWYEGSFKAGEADGHGKAYYEDGRVAYDGQWRNGVRHGLGKLYGAEGGLE
                     YFGPWRDGETLESEGPLDREAETETPIERMINTLARHPKAPVLSRAGELTVEIDCCLT
                     PHFLPLSDSGDNWNPFVATLKQYESNPNLKYEDTALKGYYESYPRENLQQSFLLSTQT
                     ELKPFWDLKPHARFPMPWTNPEAYARHLLVRGFLRYDGKALPASEQNIAEVTRMVLEL
                     AQTRQYDAWVPNDAPGMVTNLNRPTFYEFGPMPVSHGEAIYRKLVWVYESVRTVGYLP
                     GLYPGGRITGTVLLADDGYRFVADKGLFLLAALAALGYPQASATLSPETEGLIEREKI
                     RDLPFVKAGVYPADTALLLFDHAFTTFKHKIGS"
BASE COUNT         1587 a         1719 c         1865 g         1815 t
ORIGIN      
        1 gagagcgcat agagtccgga gaggtgaaac tgacggtcta cctatttcga gcggttgttg
       61 cgccagacaa agaaagagaa atgcgctgga tcatagaagc gatgcaagcg ctgaaagaag
      121 tgtcaggtag aagggatata gaagacttga tcgatctggg ctttcggtat cttctaagct
      181 tgaatctacg gatagagtta gaagagctcg tcgaaatcgc gagaaggttc tcggaagaaa
      241 ggggtgaaca gattatgacg cttgcggaga gactcatcga taaaggaatg cagaagggcc
      301 gagaaaatgg tttgaaagaa ggtttgaaag aaggcttgaa agagggcttg aaagaaggtt
      361 tagaaaaagg tttagaaaga ggattagaaa aaggccgaga ggaagaaaaa tatataatcg
      421 ctaaaagact catcctcagc gggatggact tatcggaggt ctcggtgatc accggattgt
      481 ctctcgaaga tttgcagaaa atcgatcgac tctcttagaa tagagaaaag ctgtttttcg
      541 aaaagatcac agatacgccg tggaagcatc ttgggagcgc ctgggaacgc gccgggaacg
      601 ccgcacttca gtgtggtatg tcttaaaaaa acttgggttc tctgtgcgca accttttttg
      661 tattttggag aacatcttgg aacgccacac tttagtgtgg cttgaattgt gtcttttttc
      721 atttcaaaaa ccggagaaca tgccaactta aaagttggcg ttcccagcct tacaagctcc
      781 gcgatttgtc ttcattcttt ttacgccgcg gtgatgtcgg ggtatcgcgc cgtcgcgaga
      841 agaaaaagcg cttacgccgc gatgcctgcc caaaaaccgt aaaacaaccc aactagaaag
      901 ttggcgccgc taggcgttgg caaaaatcct tgttgcttcc caaaaaaatg tacgagtgcc
      961 ttgtcataac gtcccgtgat tgtttggtgt gtcacgtatg gtatactttg ttggtggtca
     1021 agcgcgcttt ttcggccata tccaacaaaa atatcgaacc ttcgagtgaa aaggagtgtt
     1081 gattacgcgg aatgtagcga taatccccgc gcgggggggg agtaaacgga ttcctaaaaa
     1141 aaatattctg ccattcttgg gcaaaccgat catcgcttat tctatcgaaa cggcgctcga
     1201 aagtggtctg ttcgatgaag tgatggtgtc gacggacgac tcggcaattg cggatatcgc
     1261 gagagagcac ggcgcggtga ttccgtttat gcgcgaccag aacacggcca acgatcgagc
     1321 cgtaattgcg gaggtctgct ccgaggtttt acagaagtac caactccagg gtaaggagtt
     1381 cgataacgtc tgtatgatta tggcaaccgc gccttttgtc acagcccaaa gattgaagga
     1441 agcatattcc aaattgtcag aaggcaacta cgattgtatc atgaccgtta cggagtacag
     1501 ttttcctatc cagcgctctt ttaaactcga aaacgggctt cttctaatga gatggccaga
     1561 gaacatccac gttcgttccc aagatctgga aaaaacctac cacgatgccg gacagttctg
     1621 ctttttcaag gtcaattctt tccttaaaca acgcatactc ttcatggaac gaacgggaat
     1681 gatcctttta gacgccatcg aagtgcaaga tatcgataca cctattgatt gggagctcgc
     1741 cgaactcaaa taccaatttc ttcgttcgaa aaagcaatga cttcttcacc cattaactcc
     1801 caggctctca tagagcctgc actctctgtt ccctaaaacc gtgaatctta ctatgaatcg
     1861 tttcttgcgt gtgtttaagc agatcgacgc agatcatcgc cgtatcccca atcgttgaaa
     1921 accacttttg atgtattctt accgattcga ccgctgcatc catctcattc tcaaactgat
     1981 tcggtacagc gttgcgctcg atatcgaagt tgaggtaaaa agattgtatg atgtgcataa
     2041 aaaacagttg gaaattctct tttcgctcgt ggactttgac tttgtatccc gttaaggtgg
     2101 attgaaacag attgattttt tccttcgccc ttctttcatc gagcaggcct tttttaatct
     2161 cttctaacac ctcgtcgata ctcagcagcc aatccaaacc ggctttacag gtatcgtaca
     2221 cgtgttgcat atcgtcgagg gtcctgtcta ctaaccctcc aattttttct gcatcgcgat
     2281 cggcttcctc tttcgaaaat cccgaaacca aggttttaat tttgtccaac ggcgagaacg
     2341 gctcggaaat atacttatcg atcgcctcct gaaaggtcat cacggttgtt ccttttatga
     2401 aagcgccgcc ttcggtggcg ttgatgcact ggcctttgta ttcggcgaca tcgatttcat
     2461 aggacttcct aaacccattc caaatcgtgt tcgtgagtat gggctgccca tcgttcccca
     2521 taacgttaaa agtgggtaca ttatgggtcg tctgtttttc gccaaaggtc gacccggatg
     2581 cgtgcgttgt cccttcacga ctatacgcga ggtcttggcc tatcagaatt atcgggtcgc
     2641 aacccagcgc gtccagtatt ttgaacgcca tattccctga ggattgcttg atgtcgagaa
     2701 tacctcggtc gactctcaac cacttgaagt ggtcgaagtt tctatacact atgattcgcg
     2761 ggccggggta ggtttgatag gcttcgttga ataccacagg gcaggcagcg aagtaaacgt
     2821 tttctacttg ctcgacggtg aagccatcga cgagttttgc cagctccagc actcgctcaa
     2881 gcgacgtgat caaatggggt ttcacaccca tgtctaccag tatccttaag gaagcgtcgg
     2941 gagaaacgat caccgccttg tcatccaacc ctttcaagag gtgtttgttt ttgttcaaag
     3001 agggtccggt ggagacgacg atgccgggtt ttcctttgaa ctgattgtag agcaagttga
     3061 ttcccgggtt gtagatgatt tcgttgatgt tttcgagcat gttgcgcacg ccgatcagcg
     3121 agtcttcgat gctgttcccg aagttgttca attgatacgc gcccgcttca cggaaggcgc
     3181 gtagcgcgcg caagtagtat tcctttccta gtcgcatcga gctcgagtgg aagatcgggc
     3241 gcatcgcttt caacaggacg agcagcttct ggtccgcggc gaacttttct tgaagcctga
     3301 ggaagaggtc gtcttctttc tcgcccacgg cgaagtgtag gctgaaggct ttcgcgatcg
     3361 acgcgtagtc gagggtcttc agcgtcatat agaagatgtc catctctttc tcgatgatga
     3421 tgaggtgcgt ggtcccggcc tttttcacgt ggtcgcgcag gaagaggtcg agctcgtatc
     3481 ctaagccgat cccgaggaag agcgccgctt gtgtgttttt gaggttgagt ttttcgagct
     3541 cgtttcgggt gtcttgttcc gggtcggagg cggcgtagaa gaagctcttc ccgtccggga
     3601 gcaagaggtt gaggcgtggc gggtttccgg tgctcatcac acggtattgc ccggaaaggg
     3661 cgtgggtctc cacgcgctgc gcgagcgcgg gatcgcgctg cctgaggaag ttcaggttgc
     3721 tttcgaagaa gcccttcgcg actttcaccg gggtaacgct gacgacgggc ccgtccttct
     3781 tcgctgttgg gttttttatt tttttgtatc tgctcatttt tacctcctgg ttattgaatg
     3841 tgtctcgctg tggcgcattg tgagtacttc gggacttggg aacgccacac tttagtgtgg
     3901 catgggttgt gcttttttcc ttccaaaggc cggtaaaaca tgccaacttg aaaattgacg
     3961 ctcccaggcg ttttgggaac gccacatttc agtgtggcat gggctttttt ggaaaactgg
     4021 aaaacatgcc gaactaaagt tcggcgttcc caggcgctcc cagggcgttt tgagaacgcc
     4081 acacttcagt gtggcatggg ttgtgttttt ttccttccaa aggccggtaa agcatgccga
     4141 actaaagttc ggcgttccca ggcgttccag gaaacatgcc aacttgaaag ttggcgctca
     4201 cagaaaacgt tccgaactaa agttcgacgt ttccggtagt agagagctct ccataccacg
     4261 gccaatcgct tatgtctttt accaagcccg cctttattgg gttgtttttg atataacgct
     4321 ttgctctttc gaagtgggtt tcagttctca tataccggtc gtagtactct cttagccaaa
     4381 tcggtccttg cttgccgaga cgcgcgttta ttcgatacgt cgaaacctgt ttaagccgct
     4441 tgatgtattc ggatagtgta accgcggggc ttctcttcat caaaaagtgc acgtgattcg
     4501 gcataatgac ccagctgtat attgtcgcgt cttgacttaa taaagtgtcg cggaatatga
     4561 tcgcgtttcc ttcatctttt agcaggcagc ttccatagcc tttatcggca tagtcttcga
     4621 ttatctgtaa atactttctc aagcgctctt gcttgttttg atttttatcg ctttcattca
     4681 ggatgagtcg cgctcttttg acaacctcac gtggcaaaga gtcagccaat cgcaaggtaa
     4741 tgaactgaat ggcgttgttt gagtcgaaat gcggtaaata tcctcggcgt tgccactgtg
     4801 atgagtcgat gtcgttcctc ctttgacgaa gattattaaa tttcggtggt gaacgtttgt
     4861 ccgctgaata ttaatacata agacactgga aaagtcaagg acttgctttg ggaatgttgc
     4921 tctttggcgt ggcatgggtt gtgtcttttt cattccaaag agcggagaac atgccaactt
     4981 gaaagttggc gttcccagcg ttctcaaggg tatcgcgccg tcgcaaaaag aaggagcgct
     5041 tacgccgcga tgccttccca aagaccataa gacatgccaa cttgtaagtt ggcgttccca
     5101 gcgttctcaa gggtatcgcg ccgtcgcaaa aagaaggagc nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn gcttacgccg cgatgccttc ccaaagaccg taagacatgc
     5281 caacttgaaa gttggcgttc ccagcgttcc cggacgtttc cggcgttccc aggtcacgag
     5341 ccgattttgt gtttgaacgt cgtgaacgca tggtcgaaga ggaggagggc ggtgtcggcg
     5401 gggtagacgc ccgctttgac gaaggggagg tcgcggattt tctcccgttc gatgaggccc
     5461 tcagtttcgg gtgagagagt cgcgctcgcc tgggggtagc cgagcgccgc gagtgcggcg
     5521 agtaggaata accccttatc cgccacgaac cggtagccgt cgtcggcaag gagtaccgtg
     5581 cccgtgatcc tcccgcccgg gtacagccca ggcaggtagc cgacggtgcg cacgctttcg
     5641 tatacccaga cgagttttcg gtatatcgcc tcgccgtggc tcaccggcat aggtccgaat
     5701 tcgtagaagg tcgggcggtt gaggttcgtg accattccgg gtgcgtcgtt cggaacccag
     5761 gcgtcgtatt ggcgagtttg cgccagctcc agcaccatcc gcgtcacttc cgctatgttc
     5821 tgctcgctcg cgggcagggc tttgccgtcg taccggagga acccccgaac gagtaggtgg
     5881 cgtgcgtagg cttccgggtt cgtccacggc atcgggaatc gcgcatgcgg tttcaagtcc
     5941 cagaagggtt tcaactccgt ttgcgtggat agtagaaacg actgttgcag gttctcgcgt
     6001 gggtaggatt cgtagtaccc tttcagcgcc gtgtcttcgt atttcagatt gggattcgat
     6061 tcgtactgct tcagcgtcgc cacaaagggg ttccagttat ccccggagtc ggagaggggt
     6121 agaaagtgcg gcgtcaggca acagtcaatc tcgactgtca gttctcccgc cctcgagagc
     6181 accggcgcct tcgggtggcg ggctaaggtg tttatcatcc gctctatggg cgtctcggtt
     6241 tccgcttccc ggtccaaggg tccctcactt tccaaggtct ccccgtcacg ccaaggcccg
     6301 aagtactcga ggccaccttc ggcgccgtaa agctttccga ggccgtgtcg gaccccgttg
     6361 cgccattgcc cgtcataggc gacgcgaccg tcctcgtaat aggccttccc gtgcccgtcg
     6421 gcctcaccgg ccttgaacga tccctcatac caaggctgac cgttcgggtg gtacttgatc
     6481 ccttgaccct ccggatggtt cgccttccaa ccgccatcgt aggttatggc gccggcggcg
     6541 tcgtactcct tcccacgccc ctcgcgtaac ccggccttcc aggtgcccac gtaccagagc
     6601 gagccgtcgg gaaaaaatag gttcccgtcc ccgtccggca agttctgtcg ccaccccccg
     6661 cggtagtgga tggtctggcc atctcgataa taaagtgttc cggtcccgtt taggaaaccg
     6721 tcctccagtt ccccctcgta ggctttgcgg ccatcgggaa agacgatcgt tgcggtttct
     6781 ttcatctgct gatcccaccc ttaaacttgc gatgatgtga ctcgcgcggg tttctcgcac
     6841 gggtcgacta tactatatta tttgacgtca agggggtaat ttccgtgagg gggaaattta
     6901 cgggttcgca cggagaacag ggagctaaaa acaaaaaaat tgttcgcacg gagggcacgg
     6961 agagcacgga gaggacacag aggaaaaagc ctggattaaa aagatcgagt tcgcacggag
     7021 ggcacagagg gcacagagag aacacaaagg aaaattgcct tgattaaaaa cagaagattt
     7081 ggttcg
//
LOCUS       DAOPXN010000114         7042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10665_length_7042_cov_5.184056, whole genome shotgun sequence.
ACCESSION   DAOPXN010000114 DAOPXN010000000
VERSION     DAOPXN010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10665_length_7042_cov_5.184056"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..3307
                     /locus_tag="PKM49_06995"
     CDS             323..3307
                     /locus_tag="PKM49_06995"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82597.1"
                     /translation="MRVLIRKYYFFFIALLATLDAGLLFQYLSFERLLREESMSVTAL
                     TAETIEKAVSGKLRQRAQVILDAGVFTAGSAWTEESLLDFYIGLMNKNPTFMSIYYGS
                     TDNVMINGSGYIPPPTFDLRRRPWYVKAVTENRLIFTEAFINATKDQWVITIACPVYG
                     PDGKLMGVVAGDVIIGTILELIFDQENVKGTYSFLIDGKNYILAHPFIDYDLTRPLKN
                     VDELALKLLETWSHNGRSLYTAILDGKKGFLTYQGIEGTDWQIVTFFSYHDYLSYETQ
                     VLTIFTISGLASVIVFLLFLWLSKKYIWNPVLRLESEIAAIDLERSKDYRLPAGKGAA
                     FTRVRQTINHVLERAKDYFVELENHAEELAAANEQLEASFNQLALTEEEVRSKFELLK
                     HKDERLARSEKKFKSLITEMQQALVLLEIVRDENGIMTDTVFLDINRAFEQLFQTNRS
                     HVIGQRATKIAPDFVASWLLQVGRECLAGGSAQYEQYFPEMGRYFDFYSFSPEKEQFA
                     LLITDISARKADERALLESEETFRALFEESSDPVVIVQGPVFVDCNHSAVAFLGEEQK
                     EAVIGKTIWDFSPAIQPNDRPSIEAGDEVLKLAVAEGKAVFDWEFTRKDGAPLYVEVM
                     LTPITLRGESMYHCLWRDISQRRANEQRLEYLSYHDHLTGIYNRRFFMEELKRLDVRR
                     NLPITLIMADVNGLKLVNDSFGHSTGDRLLQKISTLLKKACRADDILARIGGDEFAAL
                     LPQTNAEETERIIHRMQELLSTEKINDLDLSVSFGYQSKVASEEDLLEILRKAEEVML
                     TEKLNESPLMKNRSVNVILGALYRNFEGEERHSKRVSNISRLIGSKLGLSEDDLEDLE
                     KAALLHDIGKIAIDKNIWEKNGPLTEEERAETRRHPEIGFRILATTPSLAKFSEWVWH
                     HHEWWDGSGYPKGLKGDQIPLKARVISVAEAYDRMTGSYAYRQSLTKEEAKREILRCA
                     GTQFDPAIVARLLEIIDEERFV"
     gene            3415..3491
                     /locus_tag="PKM49_07000"
     tRNA            3415..3491
                     /locus_tag="PKM49_07000"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3449..3451,aa:Thr,seq:tgt)
     gene            3505..3591
                     /locus_tag="PKM49_07005"
     tRNA            3505..3591
                     /locus_tag="PKM49_07005"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3539..3541,aa:Tyr,seq:gta)
     gene            3620..3769
                     /gene="rpmG"
                     /locus_tag="PKM49_07010"
     CDS             3620..3769
                     /gene="rpmG"
                     /locus_tag="PKM49_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNY82598.1"
                     /translation="MRVKITLECTECKDRNYSTTKNKQISKDKLSLNKYCKRCRKHTL
                     HREGK"
     gene            3781..3856
                     /locus_tag="PKM49_07015"
     tRNA            3781..3856
                     /locus_tag="PKM49_07015"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3814..3816,aa:Trp,seq:cca)
     gene            3963..4169
                     /gene="secE"
                     /locus_tag="PKM49_07020"
     CDS             3963..4169
                     /gene="secE"
                     /locus_tag="PKM49_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNY82599.1"
                     /translation="MKKPSKIKKFFREIQVEIKSTTWPNRKVLFSTAGVVAVLLAVMG
                     VYFGLLDFAFSTLTKYLLVLLGIG"
     gene            4176..5243
                     /gene="nusG"
                     /locus_tag="PKM49_07025"
     CDS             4176..5243
                     /gene="nusG"
                     /locus_tag="PKM49_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNY82600.1"
                     /translation="MKKKWFVIQTYSGLEDTIRELVDQKREALNLSHLIGRVAVPEEV
                     IIDPVNTQTERYPFSPNAKIKVSTGDEVKKGQVLGEEQPIRLRYAGEVKESRNYRKIM
                     VETLDGKYVKTYYVPESSKIESGIKSNVRIKQGMPLAREGDHVCEMDGTVLSSQKVKR
                     LVVRRKDDSKDDIYYIPLDLFVATHIKKGKAFKEGEQLCEAQPLTAAFDGLVEVIEQG
                     TRKTVKLVKIKKRRLFPGYIFIEMNMTDLSWNAIRTIPNVINFVSSGGAPIPMKDSES
                     RAIMHLAGFETPDKAAPKTAKIEFDFAVGEAIKITTGPFADFVGKISEINLEKRELKV
                     MVAIFGRDTPVFVPLNEIEKV"
     gene            5350..5775
                     /gene="rplK"
                     /locus_tag="PKM49_07030"
     CDS             5350..5775
                     /gene="rplK"
                     /locus_tag="PKM49_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNY82601.1"
                     /translation="MAKKVVTQVKLQLPAGKATPAPPVGPALGQHGVNIMGFCKQFNA
                     ETANEVGTIIPVVITIYDDRSFTFIKKTPPAAFLLLKEANLKNGSKTPLSVTAGSVTK
                     AQVRKIAEMKMPDLNARDIEAAMKTIEGTARSMGIEIKG"
     gene            5788..6474
                     /gene="rplA"
                     /locus_tag="PKM49_07035"
     CDS             5788..6474
                     /gene="rplA"
                     /locus_tag="PKM49_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNY82602.1"
                     /translation="MKTSKRFREIQKKVDITKEYELSEAVKLVKEIATAKFDETIEIH
                     FWANIDPKKAEQQIRSPISLPHGTGKTVRVLVFAKGEKAEEAKNAGADYVGAEDYADK
                     IAKENWFEFDVAIATPDMMRVIGKLGKVLGPRGLMPSPKSGTVTEDIDDAVKGFKLGR
                     IEARNDKTGNIHLVVGRRSFKDAVLQENIEATIHQVLALRPSGVKGKLFKKVSLASTM
                     SPGIRLKVAE"
     gene            6639..>7042
                     /gene="rplJ"
                     /locus_tag="PKM49_07040"
     CDS             6639..>7042
                     /gene="rplJ"
                     /locus_tag="PKM49_07040"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNY82603.1"
                     /translation="MLTRVQKETIVRELKDLFQRSPILALFDYKGLSVQKISELRDQI
                     RGLGGVLRVGKNTLLRIAIKEAGFEDTNLLNQISGTNAILYAKEDADPMQVLRAMVKF
                     AKDQPFLVPKAAIFEGQVFIGETVVDLSKLPSR"
BASE COUNT         1891 a         1669 c         1978 g         1504 t
ORIGIN      
        1 ttctctgtgc cctctgtgcg aacccccgtt tttacgcgcc gtcgcgagaa gaaggggcgc
       61 ttacgccgcg atgactacgc gaacaaaaac gtgcggttga ttttggcttt gtttcttggt
      121 tacaccccat agcttgaccc acacataacc gggtcgaacg tatatgtcaa atcatacccc
      181 ttcaccgcgt tttaccgcga tcgtattttt cggctccata atcaaaacgc cgggaaattt
      241 cgggaagtcg gatgattagt gctacaatag tgcatgaaag cgaggcgcca gtctcgatca
      301 ccgacaaacc gaggggatca ggatgcgcgt tctgatacgg aagtattatt tctttttcat
      361 agccttatta gcgactttgg atgccgggtt gctcttccaa tacctctctt ttgagagact
      421 cctgcgggag gagtcgatgt cggtgacggc cctgactgcg gaaacgatcg aaaaagccgt
      481 tagcgggaaa ctgcgccaac gcgctcaggt catcctggac gccggagtgt tcacggccgg
      541 aagcgcctgg accgaggaaa gtttactcga cttctatatc gggctgatga acaagaatcc
      601 gacgtttatg tcgatctact atgggtcgac cgacaacgtc atgatcaacg gtagcggcta
      661 tatcccccct cctacctttg acctccgacg acgcccttgg tacgtgaaag cggtgactga
      721 aaacaggctg atcttcacgg aggcctttat caacgcgacg aaagaccagt gggtcatcac
      781 gatagcttgc ccggtgtacg ggccggacgg aaagttgatg ggcgtggtcg ccggcgatgt
      841 gatcatcggg actatcctgg agttgatctt cgaccaagaa aacgtgaaag gaacctactc
      901 ttttttgatc gatgggaaga actatatttt ggcgcaccca ttcatcgatt atgatttaac
      961 ccggcctttg aaaaacgtgg atgagctggc gctgaagctg ctcgaaacgt ggtctcacaa
     1021 tggacgtagc ctgtatacgg cgatattgga cggaaagaaa gggttcctga cttatcaagg
     1081 gatagagggc accgactggc aaattgtgac gtttttcagc taccacgatt acttatccta
     1141 cgaaacgcag gttctaacga tcttcaccat atccggtctg gcctctgtca tcgtcttcct
     1201 cctcttcctc tggttgtcca aaaagtacat ttggaatcct gttctcaggt tggaatcgga
     1261 gatcgcggcg atagatctag aacggtctaa agactacagg ttgccggcag gaaaaggtgc
     1321 ggccttcaca cgggttcgtc aaacgatcaa ccacgtgttg gagagagcca aagactactt
     1381 cgtggaattg gaaaaccacg ccgaagaact ggccgctgcc aacgagcagc tggaagcgtc
     1441 tttcaaccag ttggccttga cagaagaaga ggtgcggagc aaattcgagt tgttgaagca
     1501 caaggacgag cgattggcgc gtagcgaaaa gaagttcaag tctttaatca ccgagatgca
     1561 acaggcactc gtccttctcg agatcgtacg ggacgagaat ggaattatga cggacacggt
     1621 tttcctcgat atcaaccgcg cttttgaaca acttttccag acgaaccgtt cgcacgtcat
     1681 tggccaaagg gccacgaaaa tagctccgga tttcgtggcc tcctggctgc tgcaagttgg
     1741 gagagaatgc ctagcgggag ggtcggccca atatgaacaa tacttcccgg agatggggag
     1801 atatttcgac ttctactcgt ttagtcccga aaaagagcag tttgcgctcc taatcaccga
     1861 tatctccgct cgtaaagcgg atgagcgcgc gttattggag agtgaagaaa cctttcgagc
     1921 gttgttcgag gagtcttcgg accccgtggt catcgttcag gggcccgtct tcgtcgactg
     1981 caatcattcg gcggtagcct ttttgggaga agagcaaaaa gaagcggtta ttggtaaaac
     2041 catctgggat ttttctccgg cgatacaacc caatgatcgg ccttcgatag aagcgggaga
     2101 tgaagtctta aaattggcgg tagcggaagg caaggccgta ttcgattggg aattcacgag
     2161 aaaggatggc gcgccccttt acgtagaagt gatgctgacg ccgatcacgc tgcgcgggga
     2221 atcgatgtat cattgccttt ggcgagatat cagtcagaga cgggcgaatg aacagaggtt
     2281 ggagtacttg agttatcacg accacctgac cggtatctac aaccgtcggt ttttcatgga
     2341 agagctgaaa cggttggatg ttcggcgtaa ccttccgatc accttgatta tggcagatgt
     2401 caacggcctc aagctcgtca acgactcctt cggccactcg accggcgacc gtttgcttca
     2461 gaagatatcg acgttgttga aaaaagcttg tcgcgccgat gatattctcg cgcggatcgg
     2521 aggggacgag ttcgcggcgc tactgccgca aacgaacgcg gaggaaacgg agcggatcat
     2581 ccaccggatg caagaactct tatccaccga aaaaatcaac gacctggacc tctcggtttc
     2641 tttcgggtat cagagcaagg tggcttcgga agaagacctc ctcgagatat tgcgaaaggc
     2701 cgaagaggtg atgctgacag aaaaactgaa cgaaagccca ttgatgaaga accgttctgt
     2761 gaacgtgata ttgggcgctc tctaccggaa ctttgaagga gaagagaggc actcgaagcg
     2821 cgtctcgaat atctctcggc tgatcgggag caaactgggc ctaagcgagg atgatctgga
     2881 agaccttgaa aaagcagcac tgctgcacga tatcggtaaa atcgccatag ataaaaacat
     2941 ctgggagaaa aacggaccgt tgacggaaga agagcgcgcg gaaacgcgtc ggcatcctga
     3001 gatcgggttt cgtattttgg cgactactcc gagtttagcc aagttttccg aatgggtgtg
     3061 gcatcaccac gaatggtggg acgggtccgg ttatcccaaa ggcttgaaag gggaccagat
     3121 ccctctgaaa gcgcgggtca tttcggtggc cgaggcttac gacagaatga cggggtctta
     3181 cgcctaccgt caatcgttga cgaaagaaga ggcgaagcgt gagatcctac ggtgcgcagg
     3241 gacccaattc gatcccgcga tcgttgcacg cttactggag atcatagacg aagagcgctt
     3301 cgtataacca tcagagagaa aatcacaacg cgtttgataa gtcaaaacgt tacatgggac
     3361 tattgacaaa atcttagtaa atcgctataa taccgaaggt tttcacatca acgagccagt
     3421 gtagctcagt cggccagagc ggctgatttg taatcagcag gtcgagggtt cgactccccc
     3481 cgctggctcc agtggttata gaacggtaag gtgcccgagc ggccaaaggg ggcggactgt
     3541 aaatccgctg gcagaatgcc ttcgaaggtt caaatccttc ccttaccacc aatacggaaa
     3601 tagaagaggt gtccacaacg tgagagtgaa aataaccttg gaatgcaccg aatgcaagga
     3661 taggaattat tcgacgacca agaacaaaca gatctccaaa gataagttgt cgctcaacaa
     3721 gtactgcaag agatgccgta aacacacgct ccaccgagaa ggaaagtaac gtctagaagc
     3781 aggtccgtag ctcaattggt agagcactgg tctccaaaat caggtgttgg gggttcgagc
     3841 ccctccgggc ctgccaattt ttttaagggg gaagagtttt aatgaccgat gggaaagaca
     3901 gcactcccgc aaagatcaag aaagccaaag aaccggctcg tgaagcggcg cctgcgccta
     3961 agataaaaaa accttccaag ataaagaaat tttttcgtga gatccaggtc gagataaagt
     4021 ccaccacctg gccgaatcgg aaagtgttgt tctctacagc aggagtggta gcggtcttat
     4081 tggccgtcat gggggtctat ttcgggctcc tcgatttcgc tttcagcacg cttacgaagt
     4141 acctgttggt tttgttaggc atcgggtgat tttttttgaa aaaaaagtgg tttgtcatcc
     4201 aaacctattc gggtttggag gatacgatca gggaattggt ggatcagaaa agagaggcgt
     4261 taaatttgtc ccacctgatc ggacgagtcg ccgtccctga agaggttatc atcgacccgg
     4321 tgaacacgca aaccgaaagg tatcccttct ctcccaacgc gaagatcaag gtatccaccg
     4381 gcgacgaagt taaaaaaggt caagtcctcg gagaagagca gccgatacgg ctgcgctatg
     4441 ccggagaggt caaagagagt cggaattacc ggaaaatcat ggtggaaaca ctggacggca
     4501 aatacgtgaa gacctattac gtgccggaat ccagcaaaat cgagtccggg attaagtcga
     4561 acgtacggat taaacaaggg atgccgttgg cccgagaggg cgaccacgtt tgcgagatgg
     4621 acggcacggt cttgtcctcc cagaaggtca agcgcctggt cgtgcgaaga aaagacgact
     4681 ccaaagacga tatttactac atccccttag acctcttcgt ggctacccat atcaaaaagg
     4741 ggaaagcttt caaagagggg gagcaactgt gcgaggctca gcccctgacg gccgcgttcg
     4801 acggtttggt ggaagtgata gaacagggca cgcgaaagac cgtcaaattg gtcaaaataa
     4861 aaaagagacg gctcttcccg ggttatatct ttatcgagat gaatatgact gatctttctt
     4921 ggaacgccat ccgcacgatt cctaacgtga tcaacttcgt gtcttccgga ggggcgccga
     4981 tcccgatgaa agacagcgag tcgcgggcga ttatgcattt ggccggattt gagacgccag
     5041 acaaggcggc gccgaaaacg gccaagatcg agttcgactt cgcggtgggt gaagccatca
     5101 aaatcaccac cggacctttc gcggatttcg tggggaagat cagcgagatc aacctggaga
     5161 agcgcgagtt gaaagtgatg gtagcgattt tcgggagaga taccccggtc ttcgtgccgt
     5221 tgaatgagat agaaaaggta tagtaggcgg cctataaggg ccgaagtatg tggaagggct
     5281 tcttcggaag tccgaaaatg cggaaggtcg acgatcgcgg ccttttgccc aaccaccagg
     5341 aggagaacca tggcaaaaaa agtcgtcacg caagtgaagc tgcagttgcc cgcggggaag
     5401 gccactccgg cgccccccgt tggaccggcg ttggggcagc acggggtcaa tattatgggc
     5461 ttctgtaaac aattcaacgc cgaaacggcc aatgaggtcg gcacgattat cccagtcgtg
     5521 atcacgatct acgatgaccg ttcctttacc ttcattaaaa aaacaccgcc ggccgcgttt
     5581 ttgctgctga aagaggccaa tctgaaaaat gggtcgaaga ctccattatc tgtaaccgcc
     5641 ggcagtgtga cgaaggccca agtcagaaag atcgcggaga tgaagatgcc ggacttgaac
     5701 gcgcgggata tcgaagcagc gatgaaaacg atcgaaggca ccgcccgatc gatgggtatc
     5761 gagatcaagg gctagggggc tgacgagatg aaaacatcca aaagattccg ggaaattcaa
     5821 aaaaaggtcg atatcacgaa agagtacgaa ctctccgagg cagtcaaatt ggtgaaagag
     5881 atcgcgaccg cgaagttcga cgaaacgata gagatacatt tttgggcgaa tatcgacccg
     5941 aagaaagcgg agcaacagat acgcagcccg atctcccttc ctcacggaac gggtaagacc
     6001 gtgcgcgtgc tcgtttttgc aaagggcgaa aaggccgaag aagccaaaaa cgccggggct
     6061 gactatgtcg gtgcggaaga ttacgcggac aagatcgcca aagagaattg gtttgagttc
     6121 gatgtggcga ttgcgactcc cgatatgatg cgggtgatcg gaaagctcgg aaaggtttta
     6181 ggacctcgag ggctgatgcc ctctccgaaa tcggggaccg tcacagagga tatcgacgac
     6241 gccgtcaagg gcttcaaatt gggaagaatc gaagcgcgta acgacaagac cggtaacatc
     6301 catctggtcg tgggcagacg ttccttcaaa gacgcggtgc tgcaagaaaa catcgaggcg
     6361 acgattcatc aggtgctggc tttgcgcccc tccggagtga aggggaagct gttcaagaaa
     6421 gtctcattgg cttccacgat gagcccgggc atccgcttga aggtcgccga gtaggcaaaa
     6481 taaaggaaaa atagataaaa cgcagtacac aagctgataa acggttctcg tgctgtagaa
     6541 agcgggtttt gaaaggtctt acgaccaccc gccgaggcgc gccgggtgtc tccaggcggc
     6601 ctctttcggg gccgtttttg tataggaggt gaaaacatat gttgacccga gtacaaaaag
     6661 aaaccatcgt cagagagtta aaggaccttt ttcagcgctc gccaatactc gccctttttg
     6721 attacaaagg cctcagcgtt caaaagatat cggaactacg cgaccagatc cgcggtcttg
     6781 gaggcgttct gcgggtgggg aaaaacacct tgttgagaat agccataaaa gaggcgggtt
     6841 ttgaggatac caacctcttg aaccagatca gcgggacgaa cgcaatcctg tacgccaaag
     6901 aggacgcgga tcctatgcaa gtgctgaggg cgatggtgaa attcgcgaaa gaccagccgt
     6961 ttttggtccc taaagcggcg attttcgaag gacaggtctt cataggcgag accgtcgtgg
     7021 acctttcgaa gttgccgtcc cg
//
LOCUS       DAOPXN010000115         7034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10680_length_7034_cov_4.572145, whole genome shotgun sequence.
ACCESSION   DAOPXN010000115 DAOPXN010000000
VERSION     DAOPXN010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10680_length_7034_cov_4.572145"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2628)
                     /locus_tag="PKM49_07045"
     CDS             complement(61..2628)
                     /locus_tag="PKM49_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY82604.1"
                     /translation="MISIYVPEKNDRSPFEIFVNNEKRLVESVDGRTLLGDLAPFWLN
                     PVQTAFFSHYRGKNTIISTPTGSGKTAIAFLAAFQKGKRVKKTLYVAPTRALARQIFQ
                     DIRTKPLKVYLRTGESKMDVKDDYDMVIATPEAFLAARHSNSPWVQSIEFVIIDETHM
                     LMQDTRGIVYEETLVQLLGDRRELLLLSATIPDTLSLAEWVNADLLIESSWRPIPLER
                     AFLNLALPTRKNAKTSIQQMKQAFLEENIEQRVKTMIIVPSKKTGWDLLSAFERQGYR
                     AMNDTVPYIKPKLVGEPVVAFHNADIPVEEKKEIEAAFKSLDNGLSILISTQTLAYGF
                     NSPADDIVIFVKYSLYQEEKIWPVLIDLLQFEGRAGRKGYTKRGVGRVFYATGTTGEK
                     TAAILKALLKRGLADVFETTLDKAFTALNDLYTRHESIFHPDNETKLLGMVELFSLGV
                     LSVNEEHFNYTHFKGRFKERIIQKAVSRLREIGMVEGDNRLTRLGNLTASYLLNPEAV
                     AHFVKNVTRYDVQEDPSSFWTLWESIALLVPGGGAVPDYYPPFFPPIGGHWNRQSVFF
                     SSYAGLLQFGLGFLWNRLGIEQDENDQEGRRQKPPAWVGSLPYDVELICSFYEQGARY
                     GFWDLCPSGVLERIRRSLTYGISPLYSLLTKIPGIGSLRANIVAYVGDFHRFRTDLEW
                     VRRLTQKTPPERALWEPFLPFLRTYYRVQLEWLKKSVGISGDVSIDHLIELDQANFFS
                     AVELIREYLADPEKSKTWAETPQPIWETSPLDAFKEEVKVRYPDLSLVFFVEEEGKDQ
                     LMNQVPFRLCDRSGQEKVYGTVYQAGRLPSQAVLRFQELTQRRIHTDYVDTLWEEGM"
     gene            2922..3641
                     /locus_tag="PKM49_07050"
     CDS             2922..3641
                     /locus_tag="PKM49_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNY82605.1"
                     /translation="MKKKILVVDDEPSIVELLTYNLKKEGYEVLKAYNAEEALNLADT
                     DDEIDLFVIDVMLPDVDGFDVTRKIRAHEKHQNKPIIFLTAKTDEFDKILGLELGGDD
                     YVTKPFSVREVMARIKAIFRRIQRSVEQTVERPKQIVVKDLEIDLERYEIKVRGEVKN
                     LTPLEFELLRYLAENEGKVYSRDVLLDTLWGYDYYGDTRTVDVHIRRLRTKVEEDPSN
                     PKYIITVRGKGYKFKNPGKEP"
     gene            complement(3729..4283)
                     /locus_tag="PKM49_07055"
     CDS             complement(3729..4283)
                     /locus_tag="PKM49_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNY82606.1"
                     /translation="MVWVILLIVAAAIVLWVIATYNKLVAERKKVENSWSQIDVQLKR
                     RSDLIPNLVNAVKGYMKFESDTLEKVMNARARALGATSQKERMDAEGEVSGVLGRLLA
                     VVENYPELKANQNVMSLQEELSNTENKITYSRQFYNDITMKYNTTIEQFPANLIAKLF
                     NFKMFDFFKIPEAEKEVPKVDLNF"
     gene            complement(4844..5944)
                     /locus_tag="PKM49_07060"
     CDS             complement(4844..5944)
                     /locus_tag="PKM49_07060"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNY82607.1"
                     /translation="MSEATSTRDIIRAIEEVFPPKLAMESDRCGLQVGPFDGSCERVL
                     VTLDVTEGVVEKAKEKGCRFILSHHPLLYLPCHTIDRETALGRILTEVISSEITVYAS
                     HTPADIAPGGLNDYWAEKLGLRDVKPILKTYQEKLYKFQVFVPKTHAQIVRNAMFSKG
                     AGAIGKYADCTFSSEGIGTFRPLEGAQPFIGQLNGLERVEEMKIETLVEGHRLSDFIE
                     TVLRAHPYEEVAYDLMEETEFLSNRRTYGLGRYGTVEKRAFRSFVEEFKEIARSTSIQ
                     LIGDLDDPISTVGFCSGSGRLLIPSLPAHLDLYISGDLSYHDLLGLKEKGIKVILFPH
                     FESERVFPSVVRHLLGERIPELYEYVDPVYTL"
     gene            complement(5964..6992)
                     /locus_tag="PKM49_07065"
     CDS             complement(5964..6992)
                     /locus_tag="PKM49_07065"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HNY82608.1"
                     /translation="MDAFFDAIIGWDKRRHGWEVDKAWINTCPGIVSGINTAIQAFSQ
                     PGDKVIIQPPVYHPFYDAVVGNGRQLVFNPLKKESGRFTMDFEDLEKKIDDRTKILIL
                     CSPHNPVGRVWTRDELERLAKICLNKGVLILSDEIHQDIVFSPHSHIPLASLTADARG
                     NPLPIGERTITFIAPSKTFNIAGLVTSGVIIPNRRLFAEFEYRIKANGIGIGNAFGVL
                     GLEVAYNEGEEWLNRLLPYLEANIDYTLAFLETQAPQIKCVKPDGTFLLWLDCAGLGK
                     DDSCLRNWFVEKASLGLDAGAVFGPGGEKHMRLNIGCPRSTLAEALNRLKTALSVEKP
                     AREAYFTY"
BASE COUNT         1691 a         1805 c         1829 g         1709 t
ORIGIN      
        1 caagtactta tagacgggat tccctaagta ttgaacggtg aatacctttt ccatatgaga
       61 ttacatccct tcttcccata aggtgtcgac atagtccgtg tgtatcctcc tctgagtcag
      121 ttcctgaaaa cgcaatacgg cctgactggg gagccttccc gcctggtaaa cggtgccgta
      181 gactttttcc tgtccactcc gatcacacaa tctgaagggc acctggttca tcaactgatc
      241 tttcccctcc tcttcgacga aaaaaacgag ggaaagatcg ggataccgga ctttgacctc
      301 ttctttgaaa gcgtcgaggg gcgaggtctc ccaaatgggc tgaggagttt ccgcccacgt
      361 tttcgacttt tcaggatctg cgaggtattc gcgaatgagc tcgacggcgc taaaaaaatt
      421 cgcctgatcc aattctatca agtgatcgat cgaaacgtcg cctgaaatcc caacggattt
      481 tttaagccat tccaattgca cacgatagta cgtccgtaga aacggcagaa aaggctccca
      541 aagcgccctt tcgggcggtg tcttttgcgt caacctccta acccattcta ggtctgtgcg
      601 aaaccggtgg aagtccccga cgtacgcgac gatattggcg cgcaggcttc cgatgccggg
      661 tatcttggtg agcaggctgt ataaaggcga gatcccatag gttaacgaac ggcggatccg
      721 ttctaaaacc cccgaggggc agaggtccca gaacccgtaa cgcgcccctt gttcgtagaa
      781 gctacagatc agctccacgt cataaggtaa ggagccgacc caagcgggcg gtttctgcct
      841 tcgcccctct tggtcgttct cgtcttgctc gataccgagg cggttccaca ggaaccccag
      901 tccgaattgc aacaggcccg cataggaaga gaaaaaaacg gactgccggt tccaatgccc
      961 tcctattggc ggaaaaaacg gcgggtagta gtcgggtacg gcgcccccgc cggggaccaa
     1021 gagcgcgatc gactcccaga gcgtccagaa agacgaaggg tcctcttgta catcgtaccg
     1081 cgtgacgttc ttgacgaagt gcgcgacggc ctccgggttc agcaggtaag acgcggtcag
     1141 attccccaag cgcgttaagc ggttgtctcc ctcgaccatt ccgatttccc gaagacgaga
     1201 gacggctttt tgtataattc gctccttgaa gcggccctta aaatgggtgt aattaaaatg
     1261 ctcctcatta acggagagca cgccgagaga aaacagctcg accatcccta gcagttttgt
     1321 ttcattgtcc ggatggaaga tcgactcgtg ccgggtatac agatcattca gggcggtgaa
     1381 ggctttgtct aaagtagtct cgaagacgtc ggccagcccc ctttttagca aggcttttaa
     1441 aatcgccgcc gtcttttctc cggtcgtacc ggtggcataa aagacgcgtc cgacgcctct
     1501 tttggtgtag ccttttcgac ccgccctccc ttcgaactgg agcaggtcga ttaacaccgg
     1561 ccatatcttt tcttcctgat acagagagta tttgacgaag atcacgatgt catcggcggg
     1621 ggagttgaag ccgtaggcca gggtctgcgt ggagatcagg atagatagtc cgttgtctaa
     1681 ggatttgaag gcggcttcga tttccttttt ctcctccacc gggatgtccg cgttgtgaaa
     1741 ggcgacgacg ggctcgccta ccaacttcgg tttgatgtag gggacggtat cgttcatcgc
     1801 ccgatacccc tgcctttcaa aggcggacag aaggtcccaa ccggtttttt tggatgggac
     1861 gatgatcatc gtcttcacac gttgctcgat gttctcttcc aaaaaagcct gtttcatctg
     1921 ttggatcgag gttttcgcgt tcttgcgcgt cgggagggcc aaattgagga aagcacgttc
     1981 cagagggatg ggacgccagg aactctctat caaaaggtca gcgttcaccc attcggccag
     2041 cgacagggtg tccgggatgg tggcggacaa gagcaacagt tcccgacggt ctccgagcaa
     2101 ctgaaccaga gtttcctcgt agacaatccc gcgcgtgtct tgcatcagca tatgggtctc
     2161 gtcgatgatg acgaactcga tagactgaac ccagggacta ttcgaatggc gggcggctaa
     2221 aaaggcctcc ggagttgcga tcaccatatc gtagtcgtct ttcacgtcca tcttgctttc
     2281 tcctgttcgt agatagactt tgagcggttt ggttcggata tcttgaaaga tctgtcttgc
     2341 caaagcgcgc gtgggcgcaa catacaaggt ttttttcacc cgtttcccct tctggaatgc
     2401 ggccaaaaag gcgatcgccg tctttccgct tccggtcggg gttgaaatga tcgtattctt
     2461 accacgatag tgcgaaaaga aagcggtttg aacggggttc agccaaaagg gtgcaaggtc
     2521 gcccaacaac gtgcgaccat ccacggattc gacaaggcgt ttttcgttgt tcacgaagat
     2581 ctcaaaaggc gatcggtcgt tcttttcggg gacatagata ctgatcattc tgggctcctc
     2641 gctatgatat cttggcaata ccgattatac cccataaatc gcttatcgaa acgttactcc
     2701 cgttgagaga acgaaaggcg cgtcggataa cccgtacaca cgggggtttt agatagagag
     2761 ataggcttct gaagggcgtt ttcccgtctc ctataccgtt aactgtaatt gtatcttaga
     2821 aatcagaggg ccacttgcaa tttacccgta aatatgttaa aatcgacccg gaaatcgccg
     2881 aaagataggg gtgataaaaa atagaaagga gaagacccgg cgtgaaaaag aagatactgg
     2941 tagtggatga cgagccctcg attgtggagc tgttgaccta caatctgaag aaggaaggat
     3001 acgaggttct caaagcgtac aacgcggaag aggcgctgaa cctcgccgat accgacgacg
     3061 aaatcgacct gttcgtgatc gacgtgatgc tgcccgacgt ggacggattc gacgtgaccc
     3121 ggaagatcag ggcccacgaa aaacaccaaa acaagcccat cattttcctg acggctaaaa
     3181 cagacgagtt tgacaagatt ttaggcttgg agttgggtgg cgacgactac gtgacgaagc
     3241 ccttcagcgt tcgtgaggta atggcaagga tcaaggcgat ttttcgaaga atccagcgct
     3301 ccgtggagca aaccgtagaa agacctaagc agatcgtcgt caaagacctg gaaatcgacc
     3361 tggaacggta cgaaataaaa gtgcgcggcg aagtgaaaaa cctaacgcct ttagagttcg
     3421 aactgctccg ctatttggcc gaaaacgaag gaaaggtgta ttctcgggac gttcttttag
     3481 acaccctctg ggggtatgac tattacgggg atacccgaac ggtcgatgta cacattcgac
     3541 ggttgagaac caaggtggaa gaggacccgt ccaacccgaa atatatcatc accgtacgcg
     3601 gcaaagggta taaattcaag aaccctggga aagaacccta atctcgttca cttgaaaaaa
     3661 aaccgggata acccggtttt ttttaaggct tatgattgcc catatccgaa ggatgcgagc
     3721 cgttttcttt agaaattgag atcgaccttc ggcacttctt tttcggcttc tgggatctta
     3781 aagaaatcaa acatcttgaa gttgaaaagc ttcgcgatga ggttggccgg gaactgctct
     3841 atcgtcgtgt tatatttcat ggtgatgtca ttatagaact gtctggaata ggtgatcttg
     3901 ttttccgtgt tcgacaactc ctcttgcagg gacatcacgt tttgattggc cttcaactcc
     3961 gggtaattct caacgacggc cagcagcctg cccaatacgc cggaaacctc cccttcggcg
     4021 tccattcttt ctttttggct tgtcgcaccc aaagcccgag cgcgcgcatt catcactttt
     4081 tccagtgtgt cgctctcgaa cttcatatac cccttcaccg cgttcaccag attggggatt
     4141 aagtcggagc ggcgtttcag ttggacatca atttggctcc aagagttctc gacttttttc
     4201 cgttctgcga ccagtttatt gtaggtggcg ataacccata acacaatcgc ggcggcaacg
     4261 attaatagaa tcacccaaac catgttttca tcctcctttg agcatttcta gcgattatat
     4321 cacaaagagc cagagaaaaa gagcgaggtt tgtgtcaagt tatggggtgt actcaagaaa
     4381 caaggccaaa atcgatcgca cgattttttg tttttgcagt cttcgcgacg taagcgccct
     4441 ttctttttgc gacggcgcaa taaaagtggg ttcgcacaga gggcacagag agcacggagg
     4501 agagattaga accagcccag aatagagcac cgaacgccag cctgggaacg gttgggaacg
     4561 ccaaacttta gtttggcatg gttttatctt ttcacttaga acgttcccat acgcagcatc
     4621 atggcgtaag cgccctttct tttcgcgaag gcgtaaaaaa cgggatttgc acagagggca
     4681 cagagaacac agagaaaaga tttttgtgaa cgccctggga acgccaaact tatcggcagg
     4741 tttactgagc gcgccgaatt ataagctagc agatattttg aagggacgta aaatgtactt
     4801 aagccgcgat gaacctcgta gtagaacgcg aacgcgacgc tttttaaagg gtgtagaccg
     4861 gatccacgta ttcgtagagt tccggaatcc tctcgcctaa gagatgcctg acgacggatg
     4921 ggaacacccg ttcagattca aaatgtggaa acaggatcac ctttatcccc ttctctttga
     4981 gtcccagtag atcgtgatag gacaaatcgc cggagatgta aagatccagg tgggccggaa
     5041 gcgaagggat caggagccta ccggaaccgc tacagaaccc gacggtcgag atagggtcgt
     5101 ctaggtcccc gatgagttgt atagaggtcg agcgggcgat ctccttgaat tcttctacga
     5161 aggaacgaaa ggcccttttt tcgacggttc cgtaccgtcc caggccgtag gtccgacggt
     5221 tcgaaaggaa ttcggtttct tccataagat cgtaggcgac ctcctcgtag ggatgggcgc
     5281 gaaggactgt ttcgatgaag tctgacaagc gatgcccttc gacgagggtt tcgatcttca
     5341 tttcctccac tctctccaat ccgttgagtt ggccaataaa cggttgtgct ccctccaaag
     5401 ggcggaaggt tccgatccct tcgctggaga aggtgcaatc ggcgtatttt ccgatcgctc
     5461 cggccccttt cgagaacatc gcgttccgaa cgatctgcgc atgcgtcttc ggaacgaaga
     5521 cctggaactt gtaaagcttt tcctgatagg ttttcaatat cggtttgaca tcccggaggc
     5581 ccaatttttc agcccaatag tcgttgaggc cccccggggc gatatctgcc ggcgtatggg
     5641 aggcatagac cgtgatttcg ctagagatga cttccgtaag gatccgtcca agggccgtct
     5701 cccggtctat tgtgtgacac gggagataga ggagcggatg atgcgatagg atgaaccggc
     5761 atcccttctc tttcgctttc tcaacgactc cctccgtgac atccagcgtt accaggactc
     5821 tttcgcaact gccatcgaac ggcccgactt gtaacccgca tcgatccgat tccatcgcga
     5881 gtttcggagg gaagacctct tcgatggcac gtatgatgtc cctcgtgctt gtcgcttcgc
     5941 tcatagcatc ctctgttctt cctctaatag gtaaagtagg cttctcttgc gggcttttcc
     6001 acagaaagag ccgttttcaa tcgattcagc gcttcggcaa gggtgcttcg ggggcagccg
     6061 atgttcagcc gcatatgctt ttcaccgccg gggccgaaga ccgcgcccgc atcgagaccc
     6121 aaagacgcct tttcgacaaa ccaattgcgc aagcaggagt cgtctttgcc tagccctgcg
     6181 caatcgagcc acaacaaaaa ggtgccatcg ggtttcacac actttatttg aggtgcctgt
     6241 gtctccagga aggctaaggt gtagtcgatg ttggcctcta gatagggcaa tagtcgattt
     6301 aaccactcct caccctcgtt atacgcaacc tctaagccca ggacaccgaa cgcgttccct
     6361 attccgatcc cgttggcttt tatacgatac tcaaactccg cgaacagcct ccggttggga
     6421 atgatcaccc ccgaggtgac caggccggcg atgttaaagg tcttgctggg ggcgatgaag
     6481 gtaatggttc gttctccgat tgggagcgga ttccctcggg catccgccgt tagagaagcg
     6541 agggggatat gcgagtgagg agagaagaca atgtcctgat ggatctcatc ggataggatg
     6601 agcactcctt tgttaaggca aattttcgcc aacctctcca gttcgtcacg tgtccacact
     6661 ctaccgaccg ggttgtgtgg gctgcagagg atgagaatct tggttctgtc gtctatcttc
     6721 ttctctaaat cctcaaaatc catcgtgaag cggccggatt ctttcttcaa agggttgaag
     6781 accagttgtc gcccattgcc cacgacggcg tcatagaaag ggtgatacac cggtggctgg
     6841 atgatcactt tgtcgcccgg ttgcgaaaac gcctgtatgg cggtgttgat accactgacg
     6901 atcccagggc aagtattgat ccacgctttg tcaacctccc acccgtgtct tcttttatcc
     6961 catccgatga tcgcgtcgaa aaacgcgtcc atctacgcgg tgtagccgaa aaccccgtgt
     7021 tccacacgtt tttt
//
LOCUS       DAOPXN010000116         6991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10756_length_6991_cov_4.690023, whole genome shotgun sequence.
ACCESSION   DAOPXN010000116 DAOPXN010000000
VERSION     DAOPXN010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10756_length_6991_cov_4.690023"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="PKM49_07070"
     CDS             <1..733
                     /locus_tag="PKM49_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNY82609.1"
                     /translation="HQLVFGEWVKVLEFDNAYALVKDMKTGYVGHVACNNLDFCSAEE
                     RESIRSMPKFFVGERFAYLSGLDTGFQGEKDLGWLPLGSQLFISRESEQGLYVVTPGR
                     EYWIRKHDCFVADERPVTELDDWVDKYLRVPYLWGGCSAFGTDCSGFVLRLFDMLGSS
                     LPRDTDQQIAALKEITEAELERGDLVFFPGHVGLYMGKGYIVHANVSIGGVSFSQIRD
                     PRDSYEHYLKTNITGFRRVKRQAGK"
     gene            complement(743..1516)
                     /locus_tag="PKM49_07075"
     CDS             complement(743..1516)
                     /locus_tag="PKM49_07075"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HNY82610.1"
                     /translation="MIELVKRVVVMLFLIVNLGMAFAVSYTEYVLFNRFDEAFLSARI
                     LDKGVLLIISQPRCVFSAFLRQEVLSEENVSNFLRNHFVVVEIYPDPDLTGHFNFDSY
                     ETFFSIQGKVYTYEDLYNIFLLRGTPTNAFFSKNLKLLSSFTGSVPATEYLQIAKYLS
                     QNLFQNGVDWEQYKKKEDPYIGVEEMKAITDSEKAYLLKNAKNLRTVTLDELSSSTQA
                     LDPYAYYIVEKATEEEIRGFLKQNGLTLFSVYWVKEGAE"
     gene            complement(1716..2639)
                     /locus_tag="PKM49_07080"
     CDS             complement(1716..2639)
                     /locus_tag="PKM49_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HNY82611.1"
                     /translation="MTVKVLVNDPLDASAMETLAKIPHTEIVAKHYEQAELKAVIPNY
                     HVLVVRSATKANKEIIEAGTNLKLIARAGTGLDNVDVKTAQARNITVINTPGANAISV
                     AELTIGLALSLFRFIPRGTAGLKEGKWEKKALEGCELWGKTVGIIGFGAIAQEVTKRL
                     LAFDCKILVYVTRPRTSPLNVTFVTLEELYRSSDVITIHAPLKDETKHMINEAALNKM
                     KDGVTIIHTARGGIIDEAALLKALETGKVAGAGLDVFEVEPPTDEIRKKLLAHPNVIG
                     TPHIGASTKEAQVRVGDQIVEKLRAEIAKLA"
     gene            complement(2641..3774)
                     /locus_tag="PKM49_07085"
     CDS             complement(2641..3774)
                     /locus_tag="PKM49_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNY82612.1"
                     /translation="MLKKNYLLAPGPTPVPINVLLAGAKDTIHHRTPEFVSIMESTLE
                     KAKMLFQTKEKVYCLIASGTGAMETCVVNTVAPGDKVIVVDGGKFGERWNDLCKAYGA
                     KIDLIKIEWGDYVRPEQIKEALDRNPDTVAVFTTHSETSTGTVNPLKEIAEIVRHYKA
                     ILVTDAVSAMLAEPLKMDEWGVDMVAAGLQKGVMLPPGLALVALSPKAWDRVKACGSK
                     HYYFGLKYYDKSYPDSPWTPGVNQIYQLQAALEMVEKEGVENVWKRHQILADATRAGV
                     KALGLSLLSKRPGNVTTAIFSPLDTGKLTKLMRDKYGVTMAGGQDHLKGKIVRVAHLG
                     YMGKFDVIVGLSALEMAHTEMGHPLPLGVGVKAAEEVFLKEGV"
     gene            4411..>6991
                     /gene="mgtA"
                     /locus_tag="PKM49_07090"
     CDS             4411..>6991
                     /gene="mgtA"
                     /locus_tag="PKM49_07090"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HNY82613.1"
                     /translation="MKGLIPVNFKSKKKLSKQILKNKHETEEKLRNYAFLTTQELLES
                     LSVTPAGLTTEESENRQEEYGRNIITVGKKDTVLHRLWEASFNPFNLILILIAVITYF
                     TDVVFSTQPDYLTVSIILTLVCLSSLIAFIQGQRSNKTVEKLSEMISNKADVWRDEQL
                     IQIPMDEIVPGDIIRLSAGDMLPADVRFLSTKDAFVAQSALTGESNPVEKFSDMRNKS
                     NDALTDLRNIGFMGSNMISGSARAVALATGNDTYFGSMAKSLSGDRAKTSFERGVDSV
                     SRLLVRLMLVMVPVVFLINGLAKGDWSSALLFAVSIAVGLTPEMLPVIMTSTLAKGAV
                     SMAKHKVIVRTLGAIQVFGEMDVLCTDKTGTLTEDKIILEKYMNLRGEDDTRILRHAF
                     LNSYFQTGLKNLIDHAVINRAAQYGYGNLLGFYTRVDEIPFDFSRRRMSVVLVDNNGK
                     RQLITKGAVEEMIEISSFMELDGRVLPMDEESKRVALEIYEKYNSDGLRILAVAQKNE
                     VPDVGAFSVADEKDMVLIGFVGFLDPPKESAKAAIEALRDHGVRTVVLTGDSEGVAIK
                     VCGKVSVDTSRVLTGRDVETMDDAALREAVKTCDLFAKLAPAQKKRVVKAFQAVGHTV
                     GYMGDGINDAPALRQADVGITVDSAVDIAKETADIILLKKDLMVLEEGVIEGRRTFGN
                     IVKYIKMAASGNFGNIVSVIVASILLPFLPMLPVQILTQNLLCDFSQMGMPFDSVDEE
                     YVKKPRKWDTQSIMNFISFFGPLSSIFDILCFAVLWWIIGANTKALAPIFQSGWFVYG
                     TVSQVLVIHTIRTAKIPFIQSRPTIPLIVSTFTVTTLALAVGFSHLAVGIDMRALPLL
                     FAPW"
BASE COUNT         1589 a         1794 c         1935 g         1673 t
ORIGIN      
        1 gcatcagctg gttttcgggg aatgggtcaa ggttctcgaa tttgataacg cctacgcgtt
       61 ggtgaaagat atgaaaacgg ggtatgtggg tcacgtggcc tgcaacaacc tcgacttttg
      121 cagtgccgag gagcgcgaat ccataaggag tatgccgaaa ttcttcgtcg gcgagcgttt
      181 tgcctacctc agcggtttgg acaccggttt tcagggggaa aaggacctgg gatggcttcc
      241 tctgggcagc caactcttca tctctcggga gtctgagcag ggattgtacg tggtcactcc
      301 cggaagagag tattggattc gcaaacacga ttgcttcgtc gcggacgaaa gacccgtgac
      361 cgaactcgat gattgggtgg ataaatacct gcgggtaccc tatctctggg gcggttgcag
      421 cgcgtttggg acggactgtt caggcttcgt gttgcgcttg ttcgatatgc tgggcagctc
      481 gttgccacgt gacaccgatc agcagatagc ggcgctaaag gagatcacgg aagcggaatt
      541 ggaaagagga gacctcgtct tttttcccgg ccacgtcggg ctctatatgg ggaagggata
      601 catcgttcac gcgaacgtct ctatcggcgg cgtttctttc agtcaaatcc gcgatccgcg
      661 agacagttac gagcactatt tgaaaaccaa catcaccggg tttcgccgtg tgaaaaggca
      721 ggcgggcaag tagtccgacc gattattccg caccctcttt cacccagtag acgctgaaga
      781 gggtgagacc gttctgtttg agaaagccgc ggatttcctc ttccgtggct ttttccacaa
      841 tatagtaggc gtaggggtcc aaagcctgtg ttgaagaaga gagctcgtcc aacgtgaccg
      901 tgcggaggtt tttcgcgttc ttcaataggt acgccttttc ggaatccgtg atcgctttca
      961 tttcctccac gccgatgtag gggtcctctt ttttcttata ctgttcccaa tcgacgccgt
     1021 tttgaaaaag gttttgcgag aggtacttcg cgatttgcag gtactccgtg gccgggacgc
     1081 tgccggtgaa gctggaaagc aacttcaagt tcttggagaa gaaggcgttg gttggcgtgc
     1141 cgcgcaggag gaaaatattg tagagatctt cgtaggtata gacttttcct tggatgctga
     1201 aaaaggtctc gtacgagtcg aagttgaaat gaccggttaa gtcggggtcg gggtagatct
     1261 cgacaaccac gaagtggttg cgcagaaagt tggagacgtt ctcctccgaa agcacttctt
     1321 gacgtaagaa ggcgctgaag acgcaccgcg gttgcgagat gatcagcaga acccctttgt
     1381 ccagaatccg agcgcttaaa aaggcttcgt caaaacggtt gaacaggacg tactcggtat
     1441 acgagacggc gaacgccatc ccgagattga cgatgaggaa aagcatcacg acgacacgtt
     1501 tcaccagctc tatcaccttt ctaacctttc aagaataaaa gaaaagcccg tctatccaaa
     1561 ccattctacc ctgacggagc gattcaaacg acaacaccgg cactccgtta gaagagtgag
     1621 cctagagatt agacgtaaag cagccggaaa tattcaacaa aaacagccgg cgcgagcgcc
     1681 ggccgttctt cgcttaggaa tagactgttc tatccctagg ccaatttcgc gatttctgcc
     1741 cgtaatttct cgacgatctg gtctccgacc cgcacttgcg cttctttcgt cgaggcgccg
     1801 atgtgtggtg tgccaatcac attggggtgc gcgagcagtt ttttccggat ttcgtcagtg
     1861 gggggttcca cctcgaagac gtccaatccg gcgccggcca cctttccggt ttcgagggct
     1921 ttcaggaggg ctgcttcgtc gatgatccca ccgcgggcgg tgtggatgat cgtgacgccg
     1981 tctttcattt tattcagcgc cgcttcgttg atcatatgtt tggtctcatc ttttagcggg
     2041 gcatgaatgg tgatcacgtc ggaggaccta tacagttctt ccagggtcac gaaggtcaca
     2101 ttcaaaggcg aggttctggg ccgtgtgacg tagacgagga ttttacagtc gaacgccagt
     2161 agcctcttgg tgacctcttg ggcgatcgcg ccgaagccga tgatcccgac agttttcccc
     2221 cagagctcgc agccttcgag cgctttcttt tcccactttc cttccttcaa gccggccgtt
     2281 cccctgggga tgaaccggaa caacgagagg gcgagtccga tggtgagctc cgcgacgctg
     2341 atggcattcg cgccgggggt gttgatgacg gtgatgttcc gggcttgcgc ggttttcaca
     2401 tccacattgt ccaatccggt gcccgctcgg gcaatcagct tcaggttcgt tcccgcttca
     2461 ataatctctt tgttcgcttt cgtcgcgctt ctgacgacta aaacgtggta attcggaatg
     2521 accgctttca attcagcttg ctcgtagtgt ttggccacga tctccgtgtg agggatcttc
     2581 gccaacgttt ccatcgcgga ggcgtctaag gggtcgttca cgagtacttt tacggtcata
     2641 ttagacccct tccttcagga agacttcttc ggccgctttc actccgacgc ccaaaggcaa
     2701 ggggtgcccc atctcggtgt gcgccatctc caaggccgat aaaccgacga tgacgtcgaa
     2761 cttgcccata tagcccaagt gggcgactcg gacgatctta cccttcaaat ggtcctgccc
     2821 gccggccatc gtgacgccgt atttgtctcg catcagtttc gtcagcttgc cggtatctaa
     2881 cggagagaag atcgcggttg tcacgtttcc ggggcgtttc gagagtagag aaagacccaa
     2941 cgccttcaca cccgctcgcg tggcgtctgc cagaatctga tggcgtttcc acacgttttc
     3001 tacgccttcc ttttcgacca tttcgagcgc ggcttggagt tggtagattt ggttgacgcc
     3061 gggcgtccac ggggaatccg ggtaactctt atcgtagtac ttcagaccga agtagtagtg
     3121 cttggagccg cacgctttca ctctatccca agctttcggg ctaagggcga ccagcgcgag
     3181 accgggaggc agcatcacgc ccttctgcaa accggcggcg accatgtcga ctccccattc
     3241 gtccatcttg aggggctcgg ccagcatagc gctgacggcg tcggttacga gaatggcttt
     3301 gtagtggcga acgatttcgg cgatctcttt caaggggttg accgttccgg tggaggtttc
     3361 ggaatgggtc gtgaaaacgg cgaccgtatc cgggtttcga tcgagggctt ctttgatctg
     3421 ttccggacgg acgtagtctc cccactcgat cttaatcagg tcaattttcg ctccgtaggc
     3481 tttacacaag tcattccacc gttcgccgaa cttcccgccg tctaccacga tgactttgtc
     3541 tccgggggcg acggtgttga ccacgcaggt ttccatggcg ccggttccgg acgctatgag
     3601 gcagtacacc ttctctttcg tctgaaacag cattttcgcc ttctctaaag tcgactccat
     3661 aatggaaacg aattcgggag ttctgtggtg aatagtgtct ttggctcctg cgagcaagac
     3721 gttgatcggc accggcgtgg gtcctggcgc gagtagatag ttctttttta acatataatc
     3781 cctcctaaag atgatcccat agaaatggtg tgtttccgag attatatcac taaagtcccg
     3841 aaaatccact tccgcggtat ctacgggtcg gtttttactc taatctgagg gagagagccc
     3901 gagaggtaag tttagctttt tatgatgaat gcaatatctt gcgtgcaaaa cacagaggtt
     3961 accttgggcc gcgctctccg tgccctcctc ttgaaaccct tatttttctt ttttggcatt
     4021 tctcaaaaaa atgtgacgcg caaaagcgca gcgctcccag agacctggcg aggtgtcggg
     4081 ctattcgcgg atcgagacac cggcggaagg aaggcgagtt taatccaggg cacccgcgca
     4141 aatcgtcaga ttgactttca gggcgtatcc tgatataatt cgaagagaat aaggcagaag
     4201 gaagtgagac gatggacgac aaccctcgaa gtcagagtag cggcttcaaa tccccgcacg
     4261 aaaacgaaga cccaggcgtc ggcgcacaac acgggctgtt gtccgttgct ctttgagcta
     4321 aagaactcgt aagcctgtgt gtttgtcgcc gtccgtcggg gcggcttatt tttttatgcc
     4381 cgactgcgtt ttcaccgggg aatcctaaat atgaaaggac tgattccggt gaacttcaaa
     4441 agcaaaaaaa aactcagtaa gcaaattcta aaaaacaaac acgaaacgga agaaaagctt
     4501 cggaactacg ctttcctcac aacccaagag cttctcgagt ccttgtccgt cacgccggcg
     4561 ggactcacaa cggaagagag cgaaaatcgc caggaggagt acgggagaaa catcatcacg
     4621 gtgggaaaaa aagacaccgt gctgcacagg ctttgggaag ccagcttcaa tcccttcaac
     4681 ctgattttga tcctcatcgc cgtgatcacg tatttcacag atgtggtttt ctccacgcag
     4741 ccggactatc tcaccgtctc gattatcttg acgttggtat gtctatccag tttgatcgct
     4801 ttcattcaag gccagcgatc caacaaaacc gtggagaagc tgtccgaaat gatctccaac
     4861 aaagcggacg tctggagaga cgagcagttg atacagattc ctatggacga gatcgttccc
     4921 ggcgatatta tccggttgtc cgctggagac atgctcccgg cggacgtgcg ctttttgtcc
     4981 acgaaggacg cgttcgtggc gcagtcggct ttgaccgggg agtccaatcc ggtggaaaag
     5041 tttagcgata tgagaaacaa atcaaacgac gcgctgacag atttgcggaa tatcggtttc
     5101 atgggctcga atatgatcag cggcagcgcg agagccgtgg cgctagcgac cgggaacgac
     5161 acctatttcg ggtctatggc gaaatcccta tccggcgata gggcgaagac cagcttcgaa
     5221 cgcggcgtgg attcggtcag ccggcttctg gttcgcctga tgctcgtgat ggttccggtt
     5281 gtgtttctaa tcaatggctt ggcaaaaggg gattggtcga gcgctctact attcgccgtc
     5341 agtatcgccg tggggctgac cccggagatg ttacccgtga tcatgacctc cacgctggcc
     5401 aaaggcgcgg tttctatggc gaaacacaaa gtgatcgttc gtacactcgg cgcgatacag
     5461 gtcttcggag agatggacgt gctgtgcacg gataaaaccg gtacgctgac cgaggataag
     5521 atcatcctag aaaagtatat gaacctgcgt ggagaagacg acacgcgcat tctacgccac
     5581 gccttcctta acagctactt tcagaccggc ttaaaaaacc tgatcgacca cgcggttatc
     5641 aatcgcgccg cgcaatacgg gtatggaaac ctactcggct tctatacccg tgttgatgag
     5701 atccccttcg atttttcccg acgaaggatg agcgttgttt tggtggataa caacgggaaa
     5761 cgacaactca tcacgaaggg cgcggtggag gagatgatcg agatatcctc cttcatggaa
     5821 ttggacggtc gagtcttgcc gatggacgaa gaaagcaagc gagtggcttt agaaatttac
     5881 gaaaagtata atagcgatgg gttgcggatt ctcgccgtgg cgcaaaaaaa cgaagtcccc
     5941 gacgtgggcg ccttcagcgt ggcagatgaa aaggatatgg tcctcatcgg ctttgtggga
     6001 tttcttgacc ccccgaagga gagcgccaaa gcggcgatcg aagcgcttcg agaccacgga
     6061 gtcagaaccg tggtgctcac cggagacagc gaaggcgtgg ccataaaggt ttgtggcaag
     6121 gtgagcgttg acacctccag ggtcctgacc gggcgagatg tggagacgat ggatgacgcc
     6181 gcgttacgag aggcggtcaa gacctgcgac ctgtttgcga aactggcgcc cgcccaaaaa
     6241 aaacgcgtgg tgaaagcgtt tcaggccgtg ggacatacgg tgggatatat gggggatggc
     6301 atcaacgacg cgcccgccct aaggcaggcc gacgttggaa ttaccgtgga tagcgccgtt
     6361 gacatcgcca aggaaacggc cgacattatc ctgctcaaaa aagacctcat ggtgttggag
     6421 gaaggcgtta tagaaggccg ccgaaccttc ggcaatatcg taaaatacat caagatggcg
     6481 gcaagcggga acttcggaaa tatcgtctcg gtcatcgttg ccagcatcct cctcccgttt
     6541 ctaccgatgt tgccggtaca gatcctcaca cagaacctat tatgtgattt ctcacaaatg
     6601 ggtatgccct ttgacagtgt cgacgaggag tatgtgaaaa aaccccgaaa atgggacaca
     6661 caatcgatta tgaacttcat ctcgtttttt gggcctctaa gctcgatatt cgatatccta
     6721 tgcttcgccg tgctatggtg gatcatcggg gccaacacga aggctttggc tcccatattt
     6781 caaagtgggt ggttcgtgta tggaacggta tcgcaggtat tggtcatcca tacgatacgg
     6841 accgcaaaaa tccccttcat acagagcagg ccgacgatcc ctttgattgt gtccactttc
     6901 acggttacaa cgctcgccct cgcggttggt ttcagccact tagcggttgg tatcgatatg
     6961 cgggcgctgc ccctactctt cgccccttgg t
//
LOCUS       DAOPXN010000117         6921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10897_length_6921_cov_5.773085, whole genome shotgun sequence.
ACCESSION   DAOPXN010000117 DAOPXN010000000
VERSION     DAOPXN010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10897_length_6921_cov_5.773085"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKM49_07095"
     CDS             complement(<1..238)
                     /locus_tag="PKM49_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HNY82614.1"
                     /translation="MRGKFSMKKDWVYKMPAHFLGAPFYPYRAIYPDMLAIEVSYETD
                     AERLLQYLPDVFELRTPVVSIQYSNCRDVEWMSGG"
     gene            1001..3139
                     /locus_tag="PKM49_07100"
     CDS             1001..3139
                     /locus_tag="PKM49_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNY82615.1"
                     /translation="MEEELCSFTEFDVPGPEDWKKLAIEALKGAPYEKKLVFQTDEGI
                     DVQPIYSLDDYLKNDVAEQFPGVSAFERGVEPTGYLKQVWRFAQPVRLSDPAEANRRI
                     HEQIKGGQNCVHLEIDRATRRWEDPEGTRRDSAEPTEGLNLFCREDLATVLADVPIDR
                     LPLHLYAGENPATALGLLASAFFKNGERSKIHGIVAGDPFSEWACNGLSLGSVSWQLD
                     TLPALIRWVKKHELDIKTILIRGDYYQDSGAHSALEVAIVLGLYTEYLRELLTRGVSK
                     ADAAFSIALSIGIGPNFFMEISKIRALRVLLHQVQEAFEVPGHVQGAFIHAKVARWDK
                     TLYDSYVNLLRNTSQAFSAVSAGVDSLEIPAYDALFNSSNAFSERVSRNLHHILEKES
                     RLTYPIDPAAGSWYVESLTESFCKKAWAIFQEIESLGGILKALQGGWVHKAIDASREK
                     RDKPVRTRKRPFIGTTLFANRQEKSSDFFRQGDTADPLERYRQHMKTRTHFSLPKEVG
                     KSFLKGDVAAMEAIVSFFREGGTIGTLKRLLTPGSDPTPIELLKPYRSTEAFEGIRER
                     TERWEARRGARFPLYLILYGSLTQYKARADFSEGFFEVAGFQSERTPGVRTLDEASKA
                     IEELPALPEGDAGIFVICSSDELYPEIVPPLASRLKARFPNGRVVLAGKPASGQEQAY
                     KDGGVDRWIYMGCDLVDFLTTIQNEVMPDA"
     gene            3132..5315
                     /gene="scpA"
                     /locus_tag="PKM49_07105"
     CDS             3132..5315
                     /gene="scpA"
                     /locus_tag="PKM49_07105"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014196299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HNY82616.1"
                     /translation="MPNFPDFSRMPLVLKKQRATYEEWGNLFTSAMEKNLDEMKEKTN
                     EGFVLHPLYTYDDIASLPHLRFTAGLPPYLRGPYATMYSLQPWTIRQYAGFSTAEESN
                     AFYRRNLAAGQKGLSIAFDLPTHRGYDSDHERAVSDVGKAGVAVDSILDMKILFDRIP
                     LGQMSVSMTMNGAVLPVMAFYIVAAEEQGVALEQLTGTIQNDILKEYMVRNTYIYPPE
                     TSMRIIADIFEYTAQYLPKFNSISISGYHMQEAGATADLELGYTLADGYEYVKTGIKA
                     GIDIDSFAPRLSFFWGIGMNLFVEVAKLRAARLLWAKLIKTFDPKNPKSMSLRTHSQT
                     SGWSLTEQDPYNNIARTCIEAMAAAFGHTQSLHTNAMDEAVSLPTEFSARIARNTQLY
                     LQNETEICRVVDPWGGSYYVEWLTDQLVRKAWAHIREVESLGGMAKAIETGLPKMRIE
                     EAAARKQAKIDSMSEPIIGLNLYKPEDEKPIDILEVDNTVVRENQLQRLRQLKEQRRE
                     EDVRKALDAITQATEKKDRNLLALSIEAARARATLGEISYAIEKVAGRHQALVKSISG
                     IYSSEYSDPEEIEKVRRMVSDFEKAEGRRPRILVAKMGQDGHDRGAKVISTAFADLGF
                     DVDIGPLFQTPAETAAQAVENDVHIIGMSSLAGGHKTLLPMLVEELKQRGREDIIVIA
                     GGVIPPQDYEFLKEHGASLIFGPGTLIPVAAVRILKELNERLGYA"
     gene            5308..6543
                     /gene="meaB"
                     /locus_tag="PKM49_07110"
     CDS             5308..6543
                     /gene="meaB"
                     /locus_tag="PKM49_07110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017435239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HNY82617.1"
                     /translation="MHKRIENFSSDHPVEKSGERKGSNPSLMSERGMPKTASSLPEWH
                     EEDKADRFATRVVSGAVGSVTPENSPSGTLSLGSAKTRKRRKLTLEDYLEGFEKRDKT
                     VFARAITLIESRADADRDLSQELIQHLLPKSGESIRLGFTGIPGAGKSTLIDTLGFYL
                     CEQGHHIAVLAIDPSSRLSGGSILGDKTRMEKLVGHPNAFIRPSPSGGVLGGVAQKSR
                     ETILLCEAFGYDVIVVETVGVGQSETTVHSMVDCFILLMIAGAGDELQGIKKGIVELA
                     DIIVVNKADGENLLRAKAFEREMRNVLHVISPASHGWKVPTALCSALSGEGVQELWED
                     VLRYIAMRKEQGAFLKNRQQQSLEWLHTLIGEYLRRRFYEQPAIQEALIALTKDMAEG
                     RATPASALQALIALYEHES"
     gene            6572..>6921
                     /locus_tag="PKM49_07115"
     CDS             6572..>6921
                     /locus_tag="PKM49_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNY82618.1"
                     /translation="MDYRAVYEEWLNHPLVDSKTKEELIGIRNDPKEVEDRFYRELAF
                     GTAGLRGKMAAGTNRMNPYIIRRISQALANVISESGEEAKSRGVVMAYDTRRNSNFFV
                     KIAAQIFAANGIKT"
BASE COUNT         1702 a         1856 c         1937 g         1426 t
ORIGIN      
        1 cgcctcccga catccattcc acatcccggc aatttgagta ctgaatcgac acaaccggtg
       61 ttcgcagctc aaagacatcg ggcaggtact ggagcaacct ttccgcatcg gtttcataac
      121 tgacttctat agcgagcatg tccggataaa ttgctcgata cggatagaac ggcgcgccca
      181 agaaatgggc cggcatctta taaacccagt cctttttcat actgaacttt cctcgcatat
      241 ggcacctcct aagttttttg acgtgctatc gttgctcacc gcgtaaccac aagtgatctc
      301 gacggtcgca acgaccgtac taggtgtcga atggctattt tcagaaacgg aatcagaaat
      361 gccagaaggc gaaagaaaca agaactctaa gaccttcgcc aaatggtctt ttcgtgttaa
      421 agaggggatg atgaggctgg ttccgtggct gcaacacgac cgagaaaagc gtagagacac
      481 cctcgctatc actaccccat tttttcaaag ccctatttcc tcacctcagc gtcgaaggga
      541 ctcgggtttt cctggtgcgg ggtgagggaa tggcgctaaa cagctgtatt ctaccgacaa
      601 aaacttggaa tacccggctt ggttatcctt ttaactttct ggaatccccg ggcctccata
      661 cgttataaat gcgcgcgaca atttagtcat tatctcactt tgacgaaaaa atgtcaatag
      721 cggagcgaga aaagacaaaa gggtcatcgc ggcgtaagcg cccctttttc tcgcgacggc
      781 gcgatacccc gacgtcttcg cggcgtaaaa aaacaaaagg ggatcgcggg acttcgtgag
      841 tttccgaagt cagcccgctg gttaccccac gattaacctc ttagttatgt ctccccataa
      901 cttgacccac acttcgattg aattcggcta tgggatttgg tagaatagta gcggagaacg
      961 gaagaagcgt ttcgttcctg tttttcaggg agggatcata atggaagaag aactgtgttc
     1021 ttttaccgaa ttcgacgttc cggggccgga ggattggaaa aagctggcca tcgaagcgct
     1081 gaaaggcgca ccttatgaaa agaaattggt gtttcaaacg gacgaaggga tagacgtcca
     1141 accgatctat tcgctggatg attacctcaa aaatgatgtc gcagagcaat ttccgggtgt
     1201 gagtgccttc gaaagaggag ttgaaccgac tgggtatctc aaacaagtct ggcgtttcgc
     1261 gcaaccggtt cgattgtcag atcccgccga ggcgaaccgg agaatacacg aacagataaa
     1321 aggcggtcaa aattgtgttc acctggaaat agaccgcgcc actcgtagat gggaggatcc
     1381 ggaagggact cgacgagatt ccgcagagcc gactgagggg ttaaacctct tctgcaggga
     1441 agatcttgcg accgttttgg ccgacgtgcc aatcgaccgc ctccccctcc atctctacgc
     1501 gggagaaaac ccggctaccg ccttgggtct cttggcctcg gcttttttta aaaatggcga
     1561 aaggtcgaag atacacggga tcgtggcggg agaccccttc tcggaatggg cctgcaacgg
     1621 cctatctcta ggctccgtct cctggcagct ggataccctg ccggccctca ttaggtgggt
     1681 caaaaaacac gaactcgaca taaagaccat cctcatccga ggagactatt accaagattc
     1741 gggcgctcat tcggcgttag aagtcgcgat agtcctcggt ctctacacgg agtatttacg
     1801 agaactgctg acccgggggg tgagtaaggc ggatgccgct ttttccatcg ccctttccat
     1861 cgggatcggt ccgaatttct ttatggaaat atcaaaaatc cgcgcgttac gggttctact
     1921 tcaccaagtt caagaggcgt tcgaggttcc gggacacgta cagggcgctt tcatacacgc
     1981 aaaagtggcg cgctgggaca aaaccctcta cgattcctat gtcaacctct tgcggaatac
     2041 ctcgcaagct ttttccgcgg tgagcgctgg tgtcgacagc ttggagattc cggcgtacga
     2101 cgcgctcttt aactcgtcga acgccttctc ggaaagggtg tcgagaaatc tgcaccacat
     2161 tttggaaaag gagagccgct tgacctaccc gatcgatccc gccgcggggt cttggtacgt
     2221 cgagtcgctc accgagtctt tctgcaaaaa ggcgtgggcg atattccaag agatcgaatc
     2281 tctaggtggc atactcaagg cgctgcaagg aggatgggtg cacaaagcca ttgacgcgtc
     2341 tcgagagaaa cgggacaaac ccgtccgcac ccgaaaacgc cccttcatcg gtaccacatt
     2401 gttcgcgaat cgacaagaga agtcctcgga ttttttccgg caaggggaca cggcagatcc
     2461 cctcgagcgg tatcgtcagc atatgaagac ccgcacccac ttctctctcc caaaggaagt
     2521 gggcaagtct ttccttaagg gcgacgtcgc ggcgatggag gcgatcgttt cattcttcag
     2581 ggagggcgga accatcggga cattgaagcg tttgctgacc ccgggtagcg accccacacc
     2641 catcgagctc ctgaaaccct accgctctac ggaagccttt gaagggatac gcgagagaac
     2701 ggaaagatgg gaggcgcggc gtggcgcccg attcccactc tacctgatcc tttatggctc
     2761 cctcacacaa tacaaagccc gagcggattt ttctgaaggc ttttttgagg tagccggatt
     2821 ccaatccgag cgaacgcccg gcgtacgcac gcttgatgaa gcctcgaagg cgatagagga
     2881 gcttccggca cttccggaag gagacgccgg gatcttcgtt atctgttcca gcgatgagct
     2941 ctatcccgag atcgtcccgc cactcgcctc gcgactgaag gcccgtttcc cgaacggacg
     3001 agtggtcctg gccggaaaac ccgcctcggg acaagaacag gcctacaaag acggcggggt
     3061 cgatcggtgg atctatatgg ggtgcgatct cgtcgatttt cttacgacga tacaaaacga
     3121 ggtgatgccg gatgcctaat tttcctgatt tttcacggat gccgctcgtc ctgaaaaagc
     3181 aacgggcgac ctacgaagaa tggggaaacc tttttacttc tgcgatggag aaaaatctag
     3241 atgaaatgaa ggagaagacg aacgaaggct tcgtccttca tcctctatat acctacgacg
     3301 acatcgcctc ccttcctcac ctgcgattca cggccggttt gccgccgtat ctacggggcc
     3361 cttacgccac gatgtatagt ctacaacctt ggacgatccg tcaatatgcc gggttctcga
     3421 ccgccgaaga gagcaacgcc ttctaccgga ggaatcttgc cgctgggcaa aaagggctct
     3481 cgatcgcctt cgaccttccg acgcaccggg ggtatgactc cgatcacgaa cgggccgtca
     3541 gcgacgtggg caaggctggg gtcgcggtgg attcgatact ggatatgaag atactctttg
     3601 atcgcattcc actcggccaa atgtcggttt cgatgacgat gaacggcgcc gtcttgccgg
     3661 tcatggcctt ctacatcgtc gccgcggaag aacagggtgt ggctttggag cagctcaccg
     3721 gcacgataca gaacgatatt ttgaaagagt acatggtacg caacacctat atctaccccc
     3781 ccgaaacctc tatgcggatt attgccgaca tcttcgaata tacggcccaa tacttgccca
     3841 aattcaacag catcagcatc tccggatacc atatgcaaga ggctggggcg accgccgatc
     3901 tcgagctggg atacacgtta gccgatgggt acgagtacgt aaaaaccggt atcaaagccg
     3961 gcatcgatat cgattccttc gctccccgac tctctttttt ctggggcatt gggatgaacc
     4021 tcttcgtgga ggtggcgaag ctccgagccg cccgactatt gtgggcaaag ttgatcaaaa
     4081 ccttcgatcc taaaaatccg aagtcgatgt ctctgaggac gcactctcag acctccggtt
     4141 ggagtctgac cgagcaagac ccgtacaaca acatcgccag gacttgtatc gaagcgatgg
     4201 ccgccgcttt cgggcacact cagtcgctcc acaccaacgc gatggatgag gcagtgtcgt
     4261 tacccaccga gttttccgcg cgcatcgctc gaaacacaca gctgtacctg cagaacgaaa
     4321 ccgaaatctg tcgcgtggtt gatccttggg gcggctccta ttatgtggaa tggctgacgg
     4381 atcagctcgt ccgcaaagcc tgggcgcata tccgagaggt ggaatcgttg ggcggtatgg
     4441 ccaaagcgat agaaaccgga ttgcccaaga tgcgcattga agaggccgcg gcgcggaaac
     4501 aggcgaagat cgactcgatg agcgagccga tcatcgggtt gaacctatac aaacccgaag
     4561 acgaaaaacc gatcgacatc ttggaggtcg acaacaccgt cgtgcgtgag aatcagctcc
     4621 aacggctccg gcaattgaaa gaacaacgcc gtgaagagga tgtgcgcaag gccctggacg
     4681 cgatcaccca agcgacggag aaaaaagacc ggaatctgtt ggcgctgtca atcgaagccg
     4741 cgcgggcgcg cgccacgctc ggcgagatct cttacgcaat agaaaaggtc gcggggaggc
     4801 atcaagcgtt ggtgaaaagc atatccggga tatacagctc cgaatactcg gatcccgaag
     4861 agatcgaaaa ggttcgccgt atggtgtcag acttcgaaaa agcggaggga aggcggcctc
     4921 gcatcctggt cgccaagatg ggacaggacg gtcatgaccg cggggcgaaa gtgatttcca
     4981 ccgccttcgc cgacctcggg ttcgacgtgg atatcggccc gttgttccaa accccggcgg
     5041 aaacggcggc tcaagcagtt gagaatgacg tgcatatcat cgggatgagc tcgctcgccg
     5101 gcgggcacaa gacgttgttg ccgatgctgg tggaggagtt gaaacaacgc gggcgggagg
     5161 atatcatcgt catcgccggc ggcgtgatcc ccccacaaga ctacgagttt ttgaaagagc
     5221 atggcgcgtc gttgatcttc ggtccgggca ccttgatccc tgtggccgcg gtcaggattc
     5281 tgaaggaatt gaacgagcgc ctagggtatg cataagagaa tagaaaattt ctcgagcgac
     5341 catccggtag agaagagcgg agagcggaaa gggagtaacc catcgctaat gtccgagcgg
     5401 gggatgccta agaccgcgtc ttccctaccg gaatggcacg aagaagacaa agccgatcgg
     5461 ttcgcgactc gcgtggtttc cggcgcggtc ggctccgtaa cgcctgagaa tagcccttcc
     5521 ggtacactgt cgctaggtag cgcaaagacg cgaaaaagac gaaaactgac gctggaggat
     5581 tatctcgaag gcttcgaaaa gcgagacaag acggtttttg cgcgggcgat caccttgatc
     5641 gaaagccgcg cggacgctga cagggacctc tctcaggaac tgattcaaca cctattgcca
     5701 aagtcgggcg agtcgatccg cttgggattc accggcattc ccggcgcggg aaaaagcacg
     5761 ctgatagaca ccttggggtt ttacttatgc gaacagggcc accatatcgc ggtcttggcc
     5821 atagacccga gcagtcgcct gtccggtggc agcatcttgg gggacaagac ccgtatggaa
     5881 aagttggtcg gacatccgaa cgcctttatc cggccctctc cgagtggggg cgtcttaggc
     5941 ggcgtcgcgc agaagagtcg ggagaccatc ctgctttgcg aggccttcgg atacgacgtg
     6001 atcgtcgtcg agacggtcgg agtgggccaa tccgaaacga ctgttcactc gatggtcgat
     6061 tgtttcatcc tgctcatgat cgctggggcg ggagacgaac tgcaaggtat taaaaaaggt
     6121 attgtcgagc ttgccgatat catcgtcgtc aacaaggccg acggtgaaaa cctgttgcgc
     6181 gcaaaggcct tcgaacgaga gatgcgcaac gtcctccacg tcatatcccc cgccagtcac
     6241 ggctggaagg taccgaccgc gctttgttcg gccctcagcg gggagggtgt ccaagagctg
     6301 tgggaagacg ttctgcgtta catcgcgatg cgaaaggagc aaggcgcctt cctcaaaaac
     6361 cggcaacagc aatccttgga atggctgcac accctgatcg gcgaatacct acgacggcga
     6421 ttctacgagc aaccggcgat ccaggaagcc ttgatcgcct taacgaaaga tatggcagag
     6481 ggaagagcga cgcccgcgag cgcgctacaa gcgcttatcg cactctacga acacgaatct
     6541 tgaatcgtac ctataaaagg caggtgagcc gatggactat cgagcggttt acgaagaatg
     6601 gttgaaccac ccgctggtgg acagtaaaac gaaggaagaa ctgatcggca tccggaacga
     6661 tccgaaagaa gtcgaagacc ggttttacag agaattggct ttcggaaccg cgggcctaag
     6721 ggggaagatg gcggcgggta ccaaccgtat gaatccctac atcatccggc gaatatctca
     6781 agcgctcgcg aacgtcattt cggaatcagg tgaggaagcc aagagccgag gggtcgtgat
     6841 ggcttacgat acccggcgca actccaactt tttcgtaaag atcgccgcgc agattttcgc
     6901 cgcgaacggg atcaaaacct a
//
LOCUS       DAOPXN010000118         6894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_10976_length_6894_cov_4.948530, whole genome shotgun sequence.
ACCESSION   DAOPXN010000118 DAOPXN010000000
VERSION     DAOPXN010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10976_length_6894_cov_4.948530"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..338
                     /locus_tag="PKM49_07120"
     CDS             42..338
                     /locus_tag="PKM49_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82619.1"
                     /translation="MGRLQRTKAHFFISILAYHLLNWIEQRLKETGDHRRWSTIRKTL
                     SSHQQSTVVFRDEGDKVYHLRVFGKPESPHEDIYKKLGVPMLKNRQLTYVARRL"
     gene            complement(639..2987)
                     /locus_tag="PKM49_07125"
     CDS             complement(639..2987)
                     /locus_tag="PKM49_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY82620.1"
                     /translation="MPTWKKRPRGGQNRLARLRALPRIGSLKSRLLLTLLPMVFILLF
                     LFTWLLGWGLSDLETKGAIAQSKETLMAKSETVSAWLGMIEEELQNVSYLLIDDPGKA
                     SFEHPYIRRLLNRNPVQFESFLFADTTGRALHSLGYLLNMSSNPHLNEAREKPEEFVF
                     AGPVFSELSKKPVLEIWKASVDKNGKYRGAIKATVYLDYFSEVAAGLRLTGNGAGWIV
                     TADGFFLSHPSASGALVDSVGPAIESDPLPLDERAAELLLCAIQSRCGGFRETLSPAG
                     SKDVLIYEPIANSPGWFIGVTVDQSLFFENRRRIIGFILAASAVLSICIVLSLLYASK
                     KLAAPLLALEKDIQKYGRGDLTVRVRPTGYREVLTIGRVFNEIAGRTERDFLDLEDVN
                     HRLRETNEELEYANLQLQEAGEQQHLSMDRLNEIIALTSSLSESVIRKDESFLGNLLE
                     MLMKLIPVTDFGSISVIEKEKWRFVHAVGHDLTALKKLDLSSSYLFWTAEPIVVSELL
                     GPEHNSQVPPDLMEKIRKATRPIRSSIVCRLSLGAEKLGSISLDIAEGNPIDFTPSDL
                     NIARSFSNVASAFLALQRYMVNQGKFQKELLLAMIKVLEFIDPYTKGHSENVASYCAR
                     IGEALRWPKEYITRIYWAGLVHDIGKILVPMSVLRKEAPLTEEEFETIKQHPVWGAEV
                     LKTSEELEDLAKAVLYHHERWGGGGYPKGLFGEEIPLFSRVIAVADAYDSMTSNRPYR
                     EALPEWEAMREIERNLATQFDPDIGRRFLQVIARDRKTKNNL"
     gene            complement(3041..4468)
                     /locus_tag="PKM49_07130"
     CDS             complement(3041..4468)
                     /locus_tag="PKM49_07130"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNY82621.1"
                     /translation="MAKKETTGNPVKDIHKRLRREPVSAWSRRDREEIFAFNEGYKRF
                     LFAGTTERTSVNETIRLAEENGYIPFETFLENPPKKLEGQKVYYNNRGKSVAFWVLGK
                     DIADGVNFVAAHVDSPRLDLKPAPLYEDTDLCLAKTHYYGGVKKYQWFNVPLGIDGVV
                     MKANGEKVPVRIGFEPGDPIFVISDLLPHLDRREGDITKVFQAETLNVLMGSIPLTFE
                     KTEGVKDLVLLNVLNLLNERYGFVEEDLFSAELEIVPAIEPKDVGFDRSMLAAYGHDD
                     RVCAYTALKALFDARSVSKTACTILLDKEEIGSVGSTGANHTFWKKLIKRLLPLYHTE
                     ASIDTIIERSMVISADVAAGINPSFKEVHEEKNAAKLGYGITITKYTGRGGKGGASDA
                     HAEIVGYIRRLFNEAGIVWQNGLLGKVDQGGGGTVALFFANEGLSVIDAGVAVLGMHS
                     PYEIVSKADVYETYLAYKTFFEHSK"
     gene            complement(4497..5711)
                     /locus_tag="PKM49_07135"
     CDS             complement(4497..5711)
                     /locus_tag="PKM49_07135"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HNY82622.1"
                     /translation="MKILVINTGSSSIKYQLLDMKTEQVLCKGLVERIGIPGSQLKHT
                     RGKDKYTIEKPTPHHDDGLKLILDTITDPEKGVFDNLSEIKAVGHRVVHGAEKFSGSV
                     LVESDVIHALEENAYLAPLHNPPNIVGLRAAMRLLPGIPNVAVFDTAFHSTMPAKAYL
                     YAIPMEYYRKYKIRRYGFHGTSHRYVSQVAAQMLGKDYNTMKIITCHLGNGASITAVS
                     GGKSVDTSMGFTPLEGLVMGTRSGDIDPSIIVFLQEKEGKTAEEVYGILNKKSGMLGL
                     SDGFSSDMRDIEAKVEEKEPICRLAMDIYQYRIAKYIGAYTAAMNGVDAIVFTAGVGE
                     NAPNLRMELCREYLGYLGVTIDPEKNNRRGEDIIITTPDSRTPVLVIGTNEELVIARD
                     TRDIVEKGLKSL"
     gene            complement(5736..6674)
                     /gene="fba"
                     /locus_tag="PKM49_07140"
     CDS             complement(5736..6674)
                     /gene="fba"
                     /locus_tag="PKM49_07140"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HNY82623.1"
                     /translation="MPYVPTKEILGDAYAKVYGVGAFNINNLEFLQAVIGAGVEESSP
                     VLIEVSEGAVKYAGYGQVQRGADTLYAMVKRYADTVKIPVALHLDHGKDFKYIVACIK
                     AGFGSVMIDASALPYEENLKETLRIVEIAHAVGVSVEAELGTLKGIEDNVEAAESILV
                     DPAQAEDFVKRTRVDFLAPAIGTSHGAFKFKGEARLDIRRLKKVKELTGIPLVLHGAS
                     SVPEEMVDLANRYGGKIEGAKGVPFQVLKECVEAGINKVNTDTDLRITFLGVLRKALT
                     DKPSEIDPRKLFGPAVESVKEVVKARMRVLGSSGRA"
BASE COUNT         1581 a         1898 c         1674 g         1741 t
ORIGIN      
        1 tttttgtggg gtcacccttt gcgcgttgaa gggggttgtt tttgggtcga ttacaacgga
       61 caaaggcgca cttctttatt tcgatattgg cgtatcatct actgaactgg atcgagcaac
      121 gattgaagga aacgggagac catcgacgat ggtcgaccat ccggaagaca ctctcttccc
      181 atcaacagag tacggttgtc tttcgggacg aaggggataa ggtgtaccat ttgagggtat
      241 ttggaaaacc ggaatctcct cacgaggata tctacaagaa gctgggtgtt ccgatgctta
      301 agaacaggca actgacttac gtggctcgtc gattgtagtg gccaactttt tttgaggaag
      361 tggcttttac cgccgtttgt agcgcttcta gtcgaaagta gggctgagaa gttcgccgcg
      421 accgttctga tggttgaagc gttcaatgcg cttttcctca cctaaaaata aaggtcggct
      481 tttcagccga ccgatttccg ttcgtaccgc ttgcgctaca ctcttcagtt tatcgggtac
      541 ataatcctaa gggaggaggg ggttttgctg ccgtactgtc tgggtagaac agcctttaac
      601 caccgatgcg attataaccc ctccacctta aaaagcgatt agaggttatt cttcgttttt
      661 ctatcgcgag ctatcacttg caagaaacgc cggccaatgt ccggatcgaa ctgagtggcc
      721 agatttcttt cgatttcccg catagcttcc cattcgggca gcgcctcgcg atagggacgg
      781 ttggaggtca tagaatcgta ggcgtccgca acggcgatca ctctagagaa cagcggaatt
      841 tcttcaccaa acaaaccttt ggggtaccca ccgccacccc atcgttcatg atgatacaag
      901 acggctttcg ccagatcctc gagttcctcc gaggttttca acacctcggc cccccatacc
      961 ggatgttgtt tgatcgtctc gaattcttct tccgttagcg gagcttcttt acgaagaacc
     1021 gacatcggca ccaaaatctt cccgatatcg tgcaccaaac cggcccagta gatccgcgtg
     1081 atatactctt ttggccatcg caacgcttcc ccgatacggg cgcagtaaga ggccacgttt
     1141 tctgaatggc ctttcgtata ggggtcgatg aactccagga ctttgatcat cgctaacaag
     1201 agctcttttt gaaacttccc ctgattgacc atataccgct gtagcgccag aaacgcggag
     1261 gccacattag aaaacgaacg cgcgatattc agatccgaag gcgtgaagtc gataggattc
     1321 ccttccgcga tatcgagaga gatagacccc agtttttccg cgcctaggga gagtcggcaa
     1381 acgatcgaag accgaatggg gcgggtcgcc ttccgtatct tttccattaa gtccggggga
     1441 acttgagagt tgtgttccgg gccgaggagt tccgaaacga cgatcggttc cgcagtccaa
     1501 aacagataac tcgaactcag gtcgagcttc ttcaaagcgg ttaggtcgtg tccgacggcg
     1561 tgaacaaaac gccatttttc tttctctatc accgaaatgc tgccaaaatc ggtgaccggg
     1621 atgagtttca tcagcatctc caaaaggttc cccagaaagg attcgtcttt tcgtatgacc
     1681 gattccgaaa gagaggaggt caacgcgatg atctcattca aacggtccat cgaaaggtgc
     1741 tgctgctcac cagcctcctg gagttggagg ttcgcgtatt ccagctcttc attggtttct
     1801 ctcagccgat ggttcacgtc ttccaagtcg agaaagtccc gctcggtacg gccggcgatc
     1861 tcattaaata cgcgaccgat ggttaaaacc tctcgatagc cggtgggtcg cacacgcaca
     1921 gtgaggtcgc cacggccgta tttttgtata tctttttcaa gggctagcaa aggcgcggcc
     1981 aacttcttgc tcgcgtagag caaagaaagc acgatgcaaa tagacaacac ggcggaagcg
     2041 gcgaggatga acccgataat ccgccgccta ttttcgaaaa atagggattg gtccactgtc
     2101 actcctatga accagcccgg ggagttcgcg ataggttcat agattaaaac gtctttggac
     2161 cctgcgggtg aaagcgtctc tcggaaacct ccacaccggg attggatggc acacagaagc
     2221 aattcggcag cgcgttcgtc cagtgggagc gggtcgcttt cgatggcggg tcccaccgag
     2281 tcgactagcg ccccggacgc agacgggtga gaaagaaaaa acccgtccgc cgtaacgatc
     2341 caaccggcgc cgttgcctgt aaggcgtaaa ccggccgcca cttccgagaa gtaatcgaga
     2401 taaaccgtag ccttaatggc cccacgatat ttcccgttct tgtcaacgga cgctttccag
     2461 atttctaaaa ccggtttctt ggataactcc gagaagacgg ggccggcgaa cacgaattct
     2521 tccggtttct ctctcgcttc gttaaggtgc ggattgcttg acatattgag gaggtatccc
     2581 aaggaatgaa gggctcgccc agtggtatcc gcaaataaaa aagactcaaa ttgaacgggg
     2641 tttcggttga ggaggcgccg gatataaggg tgttcgaagc tcgctttccc tgggtcgtcg
     2701 atcaagagat acgagacgtt ctgcagctct tcttcgatca tacccaacca agcggagacg
     2761 gtctcggact tggccatcag ggtttctttg ctttgagcga tcgcgccttt ggtttccagg
     2821 tccgataaac cccaacccag cagccaagta aagaggaaca gcaagatgaa aaccataggt
     2881 aaaagcgtca gaagcagcct gcttttcaga cttcctatcc gaggtaaagc tcgtaaccgg
     2941 gctaacctat tttggcctcc tctaggccgt ttcttccacg ttggcacgaa atcctcgaac
     3001 ctcctaaaaa aaagaacgcc cttaaaggcg ttctcaaccg ttacttcgag tgttcaaaaa
     3061 acgtcttata agccaaataa gtctcgtaaa cgtctgcctt gctcacgatc tcataaggcg
     3121 aatgcatccc taagaccgcc acgccggcgt cgatcaccga aagtccctcg ttggcgaaaa
     3181 atagtgcgac ggtacctcca ccgccctggt cgactttgcc tagcaaaccg ttttgccaga
     3241 cgataccggc ttcgttaaac aggcgacgaa tatagccgac gatctccgcg tgcgcgtcgc
     3301 tggcccctcc ttttccccct cggcctgtgt acttggtgat cgtgatcccg taaccgagtt
     3361 tcgcggcgtt tttctcttcg tgtacttctt tgaaggaggg attgatgcct gccgccacat
     3421 cagcggagat gaccatcgat ctttcgatga tcgtatcgat cgacgcttcc gtatggtaca
     3481 aaggcaacaa acgctttatc agctttttcc aaaaagtatg gttcgcgccc gtgctgccga
     3541 cgctgccgat ctcttcctta tccaatagaa tcgtacaggc agttttcgac acagaacgcg
     3601 catcaaacaa ggctttcaaa gccgtatagg cgcatacccg atcgtcatgg ccgtaagccg
     3661 ccaacatgct acggtcgaat ccgacgtctt tgggctctat cgcgggcacg atctccagtt
     3721 cggcggagaa gagatcctct tccacgaaac cgtatctttc gttcagaagg ttgagaacgt
     3781 tcagcaaaac caggtcctta accccttcgg ttttttcgaa agttaacggg atactgccca
     3841 ttaggacgtt gagcgtttcc gcttggaaga ctttcgtgat atccccttct cgtcgatcga
     3901 gatggggtaa caggtcggag atcacgaaaa tcggatcacc gggctcgaac ccgatccgaa
     3961 cgggcacctt ttccccgttc gctttcatca cgacgccatc gatccctaag ggaacgttaa
     4021 accattggta tttctttacc ccgccatagt aatgcgtttt cgccaaacac aagtcggtat
     4081 cctcgtacaa aggcgccggt ttgagatcca aacggggaga gtcgacgtgc gcggcgacaa
     4141 aattgacccc atccgcgata tctttcccca aaacccaaaa agcgacggat ttgccacggt
     4201 tgttgtaata cactttctga ccttccaact tcttcggagg attctccaga aaggtctcga
     4261 acggaatata gccgttttcc tccgccaatc gtatcgtttc gtttacggac gtcctttcgg
     4321 tagtcccagc gaacaagaac cgtttgtacc cttcgttaaa ggcgaagatc tcctctctat
     4381 ctcgcctaga ccacgccgat accggctccc gtctcagccg tttatgtatg tctttcacgg
     4441 gatttccggt ggtttctttt ttcgccataa ctcactcctt cgttttctct atttgattaa
     4501 agcgacttta atcctttttc gacgatatcg cgcgtatctc gcgcgatcac aagctcttcg
     4561 ttggttccga tcaccaatac gggagttctc gaatcgggag tcgtgatgat gatatcttct
     4621 ccacgtctat tgttcttttc gggatcgatc gtgacgccta agtagcccaa gtattcgcgg
     4681 cagagctcca tccgtaaatt cggcgcgttt tctccgacac cggcggtgaa gacgatggcg
     4741 tcgaccccgt tcatagcggc cgtatacgcc ccgatgtatt tcgcgatacg gtattggtaa
     4801 atatccatcg ccaagcggca gattggctct ttttcctcca ctttcgcttc gatatcccgc
     4861 atatcactgc taaacccgtc cgataggccg agcatcccgc tcttcttgtt caggataccg
     4921 tagacctctt cggccgtctt accctctttt tcttgtaaaa aaacgataat cgaggggtca
     4981 atgtctccgc tccgtgtgcc catgactaac ccctccaaag gagtgaaacc catagaggtg
     5041 tcgaccgatt tcccgccact gaccgcggtg atcgaggcgc cgtttcccaa gtgacaggtg
     5101 atgattttca tcgtgttgta atcttttcct aacatttgag cggcaacctg agagacatag
     5161 cggtgagatg tcccgtgaaa accgtaccgg cgtatcttgt acttacggta atactccata
     5221 gggattgcgt acaaatacgc tttcgcgggc atcgtactat ggaacgcggt atcgaaaacg
     5281 gccacgttgg ggatgcccgg aagcagccgc atcgcggcgc gtaatccgac gatgttcggc
     5341 gggttgtgca acggcgccaa ataggcgttt tcctccaaag cgtgtatgac gtcgctttcg
     5401 accaaaacgg agcccgaaaa cttttcggcg ccgtgcacca cacggtgacc aaccgcttta
     5461 atctcgctca agttgtcgaa cacgcctttc tcggggtcgg tgatcgtatc tagaatcagt
     5521 ttcaacccgt cgtcgtggtg gggcgtcggt ttctcgatcg tatacttgtc ttttcctcgt
     5581 gtatgcttca gttgcgatcc cggtatgccg atacgttcga cgagcccttt gcaaagcact
     5641 tgctcggttt tcatatccag cagctgatac ttgatcgagg agctcccggt gtttatcacc
     5701 agtattttca cgtcattcct cctccaaacc ccggctcagg ctctacccga tgagcctaag
     5761 acgcgcatcc tggcttttac gacctcttta accgattcta ccgccggtcc gaacagttta
     5821 cgcgggtcga tctcgctggg cttgtccgtt aacgcctttc tcaacacccc aagaaaggtg
     5881 attctgaggt cggtatcggt attgacctta ttgataccgg cttccacaca ttcttttaaa
     5941 acctggaatg ggacaccttt cgcgccttcg atctttccgc cataccgatt ggccagatcg
     6001 accatttctt cgggcaccga agaggcgccg tgtaggacta acggaatccc cgtcagttcc
     6061 ttcacctttt ttagccttcg tatgtctaac ctggcttccc ctttgaactt gaaagcgccg
     6121 tgactggtgc cgatagcggg cgcgagaaag tctacacggg ttcttttcac gaagtcctca
     6181 gcctgagcgg ggtctactaa aatggattcg gcagcttcca cattatcctc gatccccttc
     6241 agggttccca attccgcctc caccgatacg cctacggcgt gagcgatttc gacgatgcgt
     6301 agggtttctt tgaggttttc ttcataaggc agagcggacg cgtctatcat taccgatcca
     6361 aagcccgctt tgatacaggc tacgatatat ttgaaatcct tcccgtggtc caggtgcaaa
     6421 gcgaccggga tcttcaccgt atccgcatac cgcttcacca tcgcatagag tgtatcggcc
     6481 cccctttgaa cctgaccgta cccggcgtac ttgacagccc cttcactgac ctcgatcaga
     6541 acaggcgaac tctcctctac acccgctccg atcacggctt gaaggaactc tagattgttg
     6601 atattaaaag cgccgacacc gtataccttt gcataggcgt caccaagaat ctctttcgtc
     6661 ggaacatagg gcataccttt gaacctcctt atataacagt cgaagaaaac ggtatcgtct
     6721 tcctcactga atgtatgatc tccatatggg ccccaagctg aggtgcatat aggcgccctc
     6781 caaacgaacc atttctcctg agggcacgat gatgacctgc caaggtgggc agcgaacgtg
     6841 gtctacaatt aggtgttctt gtgaaagcat gaggcaaaag ccttctgcgg ttcc
//
LOCUS       DAOPXN010000119         6739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11290_length_6739_cov_5.019449, whole genome shotgun sequence.
ACCESSION   DAOPXN010000119 DAOPXN010000000
VERSION     DAOPXN010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11290_length_6739_cov_5.019449"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKM49_07145"
     CDS             complement(<1..833)
                     /locus_tag="PKM49_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82624.1"
                     /translation="MPLNVIIKELVEGLPSQVRSHFFSRSKKEKSISMVLDHAKELLK
                     PVVHTLWNELEVKEQEVRAYVSQKLTAGVSQERILAELKAKEDPDQVTLFQLVYEEVK
                     KQVEDEKFFIFKEVGERIADREIWNLVHARIPPDDRVMATVSGSLKSECLVKTAKQII
                     IVKKGISSLLKGSLQTSIQAIPIDQILDVSISGSMIGAVMSLKYLSGSTDFRFKKEYQ
                     TEMEGIILPIRERLKKLEKVEPTAPTPYLKAIEGPEAEIGAPEPQMGPEPAVSLTSTP
                     F"
     gene            complement(1435..2898)
                     /gene="xylB"
                     /locus_tag="PKM49_07150"
     CDS             complement(1435..2898)
                     /gene="xylB"
                     /locus_tag="PKM49_07150"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HNY82625.1"
                     /translation="MFLGIDIGTTSIKALVVQEEGKIMDSFSRPLQVFSPDSLSREQD
                     PQHWQEAVLALLHQVSERHEIVSIGLAGQMHSLVLLDDRDRPLRPSILWCDQRTTEEC
                     RKLTEKLGGESEVIRRVGNPFFEGFTLPKIQWVKTHEPENYRNACSILLPKDYIGFLL
                     TGEKGTDPSDASGTGAFCVNAEKWDDELLTLCEIAPSLFPKVTPSDSLRGFLRKDLSE
                     RFGWEKVRVITGGADNPVAALGAGVDDPGLGMISLGTSGTVVVSTKSEVPDPTGRVHY
                     FHHALPRIRYYMGVMLSATHSLDWFLSRFGKKDSWSEFETAIGNSVAGSRGLLFLPYL
                     NGERTPHRDPDARGVLFGISSTHTEGEAYRCILEGIAYGLRDSYELIKALTPIKRLRV
                     VGGGAKNRTWVRIIASNLSTPLELLETDQGAAYGAAMLAANGSGLSKEETSRWARVAE
                     VIDPDPLWEKAYREGFHRYQRLYSTLKDTFASSIQKT"
     gene            complement(2936..4132)
                     /locus_tag="PKM49_07155"
     CDS             complement(2936..4132)
                     /locus_tag="PKM49_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HNY82626.1"
                     /translation="MIDGTMNAEKMGISNRKTVFQLIRNVPDISRKELSESTGLDPST
                     ISRIVSSFRKQGYVQETGLNRSTSPGRSSVRLSVIKEAAISYLISLGVEKTILGLGFL
                     DHSVQRIEEFATPRGFDLFLKTLADKISKHFREFPTKNRTIAMSFSLPGIIDRKNRYI
                     YNLPHLGWTGIQLREAVHRVLGETRLEVLAANEAQLSLMAERYLNDTLTPYKHGFYLF
                     LSQGIGGAILSEGDLYLGRSFSAGEAGHMRIDKNGALCQCGGRGCLETLAAIEPVVRD
                     YERKGTALYGANSLEKFRSLISRYRQGDPLAIEGLSAMQDNLVLGITNIANLLNPEFL
                     MLGGMGYDLPEEWTSILEGRVKKEALTSAGKGLKILRATMDIYDSTLLGSDLLAMDYF
                     TEKNIR"
     gene            complement(4194..5156)
                     /locus_tag="PKM49_07160"
     CDS             complement(4194..5156)
                     /locus_tag="PKM49_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82627.1"
                     /translation="MKKALLMVLVVTLLLSSFAFSAPKIRIGVSIPTADHGWTGGINW
                     WAQKAIKEWQAKDPDVTFFLVTADSPAKQVADVEDLMVKGIDALVILAHDSAPLTPIV
                     EKVYNEGIFVVSVDRGLTKPVEHVYVAGDNPGLGRVSAEWMAKELNYKGSIVVLEGIP
                     CVINSERVDAFNEVMKKYPNIKILDSQPAYWSTQKGLEIMENYLQKYDKIDAVWAQDD
                     DVLKGVLQAYKESRRKDILFFLGGAGSKEIIKMVMDGDKQVRADVTYPPSMIGTGISM
                     AVMSLRGEKLNGFYQNQIPSKIILAAELITVGNAKSYYEPDSIF"
     gene            complement(5195..6163)
                     /locus_tag="PKM49_07165"
     CDS             complement(5195..6163)
                     /locus_tag="PKM49_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012069561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82628.1"
                     /translation="MTIKLLLSKIRRIDFEKYGPLFALAILFIISAIASPYFIKTRNL
                     LNILRQVSYTGIIGLGMTFVIISGGIDLSVGSMTALVGGIMILTLNHFGGGVSAILMV
                     LLVGILLGAGAGALNGIIITKGRVAPFIVTLGTMAVYRSLTLYISNAGEFRSNSNLYP
                     ELGMGAFLEIPNPVWIFFLLAILCSIILNYTKFGRYVRAVGSNDKVATYSGIRVDRVR
                     FLTYVLVGVMVAVSALLLSSRLNSISSSNAAVNYELDAIAAVIIGGTAMSGGSGTVFG
                     TVIGAIILGIVNNMLNMLGISPYLQGTVKGLVIIGAVLIQKKKKRS"
     gene            complement(6166..>6739)
                     /locus_tag="PKM49_07170"
     CDS             complement(6166..>6739)
                     /locus_tag="PKM49_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY82629.1"
                     /translation="GTILLNGKEVYVKNPTRAFASGLAYLSEDRQGSGILTAFGITEN
                     ITLTSLKRYSRFLLKKKEEALKAQEYVRKFDIKAASLKTRLEFFSGGNQQKVSFAKSL
                     DSDPQIILIDEPTRGIDVQAKHEMYRFIHSLVEGGLSCVLISSEMEEVIGMSNRVVVM
                     KEGRITGILTGDQINEEEIMFYATGLKGVA"
BASE COUNT         1541 a         1812 c         1692 g         1694 t
ORIGIN      
        1 tcgaatggtg ttgacgtaag gcttacggcg ggttctggcc ccatttgggg ttctggggct
       61 ccaatctccg cttcaggtcc ttcgattgcc ttcaaatagg gggtgggcgc tgtaggttcc
      121 accttctcca gcttcttcaa ccgctctcta ataggcagaa tgataccttc catttcggtt
      181 tgatactctt ttttaaaccg gaaatccgtc gatccgctca aatacttaag gctcatcacg
      241 gcgccgatca tactgccgct gatggagaca tccagaatct ggtctatcgg tatggcttgg
      301 atagaagtct gtaaagagcc cttaagaagg gaggaaatac cttttttgac gattatgatt
      361 tgtttggctg ttttaacgag gcattcggac ttgagggagc cactgaccgt agccataacc
      421 cgatcgtccg gggggatcct ggcgtgtacg agattccaga tctctcgatc cgcaattcgt
      481 tctccgactt ctttgaagat aaaaaacttc tcgtcttcca cttgcttttt cacttcttca
      541 tagaccagtt ggaagagggt tacctgatcc gggtcttctt tggctttgag ctcggctaaa
      601 attctttctt ggctgactcc ggcggtgagt ttttgtgaca cgtaggcccg aacctcttgc
      661 tctttaacct ccaattcgtt ccacagcgta tggaccacag gcttcaacag ttccttcgcg
      721 tgatcgagga ccatagatat gcttttttct tttttggacc gactgaaaaa atggcttctg
      781 acttgagaag ggagtccttc caccaactct tttatgatga cgttcaacgg cattctatac
      841 acctcctgaa gacgttatgc cgagtgtcga cgccgatttt ctcgagcatc cggtacgatt
      901 atagcacagt gtcggtttcg aaaactccct tgtgtgggtc aagttgtgga gtgacataac
      961 tgagaggttc atcgcgaggt ccctttggtt accccgcgat aacccgcgaa gtttggcgtt
     1021 cccagggctt tcccagcgaa atttctttgg cgcactctag tgaaaatctt ggtgttctct
     1081 ctgtgttctc cgtgtactct gcgcgaaccc tgttctatct ttttaagata tgacaactaa
     1141 aaagttagcg ttctcagggc ggatcgcgag acttcgtgga cttccgaagt cagcccgctg
     1201 gttaccccgc gatgacccgc gaagtttggc gttctcaggg tatctccaga gacaggccga
     1261 ctggaagtcg gccccacgaa gagaaggagc gcttgcgccg cgatgactac gtgggctaaa
     1321 aatcgcgcga acaattttgg tcttatttct tgattacacc ccacaacttg acccacacat
     1381 gcccttagga aaataacacc gatgacaggt attcggtcag acgatgaacg gttcttaagt
     1441 cttttgtata gaggaagcga acgtatcttt gagggtagaa tagagccgtt gataccggtg
     1501 aaaaccctct cggtaggcct tttcccaaag gggatcgggg tcgatgactt ccgcgacccg
     1561 agcccaacgg ctcgtttctt cttttgagag gcccgagcca ttcgcagcca gcatagccgc
     1621 cccataagcc gcgccttgat cggtttcgag taactccaaa ggcgtcgata ggttcgacgc
     1681 gatgatccga acccacgttc tgttcttcgc gccgcctcct acgacacgaa ggcgtttaat
     1741 cggcgtgagg gcctttatca attcatagga gtctcgcaat ccgtaggcga tcccttcgag
     1801 gatgcatcgg taagcctcac cctcggtgtg tgtggaagag atgccaaaca aaacccctct
     1861 cgcatccgga tccctgtgag gtgttcgctc gccattcaag taaggcagga atagaaggcc
     1921 acgagagcca gccacgctgt ttccaatggc ggtttcaaac tcgctccaac tgtccttttt
     1981 cccgaaacga cttaaaaacc agtctagcga gtgagtcgcg gagagcatca cacccatata
     2041 gtagcggata cgggggaggg cgtggtggaa ataatgaacc cgcccagtgg gatcggggac
     2101 ctcggactta gtggacacga ccaccgttcc ggaggtgccc aagctgatca ttccgagtcc
     2161 tggatcatcg acgccggcgc ccaaggcggc gacggggttg tccgctccgc cggtgatcac
     2221 gcgaaccttc tcccaaccga aacgttcaga aaggtccttt cgtaaaaagc ctcgcaagct
     2281 atccgagggg gtgactttcg ggaacaacga gggagcgatc tcgcagaggg tcaacagctc
     2341 atcatcccat ttctcggcat tgacgcagaa ggcgccagta cccgacgcgt cggaggggtc
     2401 ggtgcctttt tcccccgtta gaaggaagcc gatgtaatct ttggggagta agatcgaaca
     2461 ggcgtttcga tagttttccg gctcgtgggt tttcacccat tgaatcttcg gtagcgtgaa
     2521 cccctcgaaa aaggggttac ccacgcgtcg gatcacctcg ctttctcctc ccagtttttc
     2581 ggtaagcttc cggcactcct ccgtcgtgcg ctggtcacac catagaatgc tggggcgtaa
     2641 cgggcggtct cgatcgtcca ataaaaccaa cgaatgcatc tggccggcta acccaatgct
     2701 gacgatctcg tgccgctctg aaacttggtg caatagtgcc aaaaccgctt cctgccaatg
     2761 ttgtgggtcc tgctctctag aaaggctatc cggtgagaag acttgaagcg gtctcgaaaa
     2821 agaatccatt atctttccct cttcttgaac aacgagggct tttatggagg tcgtgccgat
     2881 gtctatgccc aaaaacatac cttcacctcg cgatgccacg ccacggtggt gagactcagc
     2941 ggatattttt ctcagtgaag taatccattg ccaacaaatc gcttccaagc agggtggaat
     3001 cgtagatatc catcgtagct cgcaggatct tcaacccttt ccctgcgctc gtcagcgctt
     3061 ccttttttac acggccctcc aagatcgacg tccattcttc gggcagatcg tagcccattc
     3121 ctcctaacat caggaactcc gggttcaaga ggttggcgat gttcgttatc ccgaggacca
     3181 agttgtcttg cattgcggac aacccttcga tcgccaaggg gtcaccctgt cggtatcggg
     3241 agatcagaga acggaacttt tctaaggagt tggcgccgta caatgccgtt ccttttcgct
     3301 catagtctct gacgaccggc tcgatcgcgg cgagggtttc caagcagccc cgtccgccgc
     3361 actggcataa tgcgccgttt ttgtcgatgc gcatatgccc cgcttcgccc gcggaaaaag
     3421 agcgtcccaa gtagaggtct ccttcggaca ggatcgcgcc gccgatccct tgtgataaaa
     3481 acaagtagaa tccgtgtttg tagggggtca gggtgtcatt caaatagcgc tcggccatta
     3541 gggagagttg cgcctcgttg gccgctaaaa cctccaatct cgtctcgcct aggacacgat
     3601 gaaccgcctc cctcagttgg ataccggtcc acccgaggtg cgggaggttg tagatgtaac
     3661 ggtttttcct gtcgatgatg ccgggtaaag aaaagctcat cgcgatggtt cggtttttcg
     3721 tcgggaattc gcggaagtgt ttgctaatct tatccgccag ggttttcaag aagaggtcaa
     3781 agcctctggg cgtcgcgaat tcttcgatcc tctgaacgct atggtcgagg aagcctagac
     3841 caagaatcgt cttttcgacc cccaagctga tcagataaga gatcgcggct tcttttatca
     3901 cggaaaggcg cacggagctc cgcccgggag aggtcgaccg gttaaggccg gtttcttgta
     3961 cgtagccttg ctttctaaaa gaggacacga tcctcgagat cgtgcttgga tccaaccccg
     4021 tactctccga tagctccttt cgcgagatat ccggcacgtt gcgaatcagc tgaaagaccg
     4081 tttttctgtt ggaaatcccc atcttttcgg cgttcatagt cccgtctatc ataggttccc
     4141 ttctctttca aagcgccggg ggacgcatcc cccggctgta tagcttccgc ggtttagaaa
     4201 atcgagtctg gttcgtaata actcttagcg tttccaacgg tgatgagttc ggccgctaga
     4261 atgatcttgg acggaatttg attttggtaa aaaccattca gcttctcgcc gcgaagcgac
     4321 atcaccgcca tactgatgcc ggttccgatc atcgaaggcg ggtaagtgac gtccgcgcgc
     4381 acctgtttgt ccccatccat aaccattttt attatctctt tggaacccgc gccgccgagg
     4441 aagaacagga tgtcctttct cctggattcc ttataggcct gcaacactcc tttcagaacg
     4501 tcgtcgtctt gtgcccaaac ggcgtcgatt ttgtcgtact tttgcagata attttccata
     4561 atctccagcc ctttttgtgt cgaccaataa gctggctggg agtcgaggat tttgatattg
     4621 gggtattttt tcataacttc gttaaaggcg tctacccgtt cggagtttat cacgcacgga
     4681 ataccttcca acaccacgat gctcccctta tagttcagtt ctttggccat ccattcggcg
     4741 gatacgcgac ccagacccgg gttgtctccg gcgacgtaaa cgtgttctac gggtttggtc
     4801 aatccgcggt cgaccgaaac gacgaagatg ccttcgttat agactttttc aacgatcggt
     4861 gtcaaagggg cagagtcgtg ggctaaaatg actaacgcgt caataccctt aaccatgagg
     4921 tcctcgacgt ctgcaacttg tttcgcgggc gagtcggcgg tcacaaggaa aaacgtcaca
     4981 tccgggtctt tcgcttgcca ttccttaatc gccttttgcg cccaccagtt aatacctccc
     5041 gtccacccgt gatcggcagt cggaatagac acgccaatgc ggatcttcgg ggccgagaac
     5101 gcgaaagaag agagcaacaa cgtcaccaca agaaccatta acaacgcttt tttcatagat
     5161 tcgtgcacct ccttataaga taccctaata gggtttacga ccttttcttt ttcttttgta
     5221 tgagaacggc tccgataatg acgagccctt ttaccgttcc ttgtaaatac ggtgagatac
     5281 cgagcatgtt caacatattg ttcacaattc cgaggataat cgcgccgatc accgtcccga
     5341 acactgttcc gctaccgccg ctcatcgcgg tcccgccaat gatcacggcc gcgatcgcgt
     5401 cgagttcgta gttgacagcc gcatttgaag acgagatcga gttaagtcgg gaagagagca
     5461 gcagagctga cacggcgacc atcacgccga cgagaacata ggttaggaac cggacgcgat
     5521 ccacccgtat accagaatag gtggcgactt tatcgttaga gcctaccgcc cggacgtaac
     5581 gcccaaactt cgtatagttc aggatgatag agcacagtat agcgagaagg aaaaatatcc
     5641 agacgggatt cggtatttct aaaaacgctc ccatacccaa ctcgggatag aggttactgt
     5701 tcgagcgaaa ctctccagca ttgctgatat acagcgttag cgatcgatat acagccatcg
     5761 tgcccagcgt aacgataaaa ggagccaccc gacctttggt gatgatgatc ccgttcaaag
     5821 ccccagcgcc cgccccaagt aggattccga ccagaaggac catcaggatg gccgagacgc
     5881 ctccaccaaa atgattcaat gtcaggatca taatgccacc gaccaacgcc gtcatcgaac
     5941 caacggagag gtctatccca ccggaaatga tgacgaaggt cattcctaaa ccgatgattc
     6001 cggtgtaaga aacctgacgc aagatgttta gaaggtttcg cgttttgata aaatacgggc
     6061 tggcaatcgc ggagatgata aacagaatgg cgagggcaaa gagcggaccg tatttctcga
     6121 agtctatcct ccgtatcttg gacaacaata gtttgatcgt cattctcaag caaccccctt
     6181 taatccggtg gcatagaaca taatttcttc ttcgttaatt tgatctccgg ttaaaatccc
     6241 ggtgatccgc ccttctttca tcaccaccac gcgattggac attccgatga cctcttccat
     6301 ttcagacgag atgagaacgc aggataaacc gccctccacc agcgaatgga tgaacctgta
     6361 catctcgtgt ttagcttgca catcgatccc gcgggtcggt tcgtcaatca ggatgatctg
     6421 ggggtccgaa tcgaggcttt tggcgaaaga gaccttttgc tggttacctc cgctgaagaa
     6481 ttcgagccgc gttttaagcg aagcagcctt gatgtcgaac ttgcggacat actcttgcgc
     6541 tttgagcgcc tcctccttct ttttgagaag gaaacggctg taccgtttta aggaggtgag
     6601 agtgatattc tcggtgatcc cgaaggcggt taggattccg ctcccctgtc ggtcctccga
     6661 gagataggcg agtccgctgg cgaacgcgcg cgtcggattc ttaacatata cctctttccc
     6721 gttgagtaaa atcgtaccg
//
LOCUS       DAOPXN010000120         6705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11358_length_6705_cov_4.996541, whole genome shotgun sequence.
ACCESSION   DAOPXN010000120 DAOPXN010000000
VERSION     DAOPXN010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11358_length_6705_cov_4.996541"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            636..1580
                     /gene="arcC"
                     /locus_tag="PKM49_07175"
     CDS             636..1580
                     /gene="arcC"
                     /locus_tag="PKM49_07175"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HNY82630.1"
                     /translation="MPKTLAVVAIGGNAVNKPKESATAENMFRNLSVTAEYLMQMLKD
                     EYELVITHGNGPQVGNILIQQEAGKEKIPPFTIDLNDAQTQGSLGYAIALTLRNALLQ
                     ANHPREICSVITQIVVDPKDPAFQNPTKPIGPFYSEEEGKRIQEEKGWVFVEDTGRGW
                     RRVVPSPIPLDIIEKKAIKQLVKAGIITIAAGGGGIPVNRLPDGSLQGVEAVIDKDRA
                     SALLALELNADMFIILTGVEKVYSDYGKPTQKAFDTLTVEEANRYLHGGQFPKGSMGP
                     KIEAAIAYVSKTGKRCLITAMETLGDALKGKTGTWIVN"
     gene            1718..2935
                     /locus_tag="PKM49_07180"
     CDS             1718..2935
                     /locus_tag="PKM49_07180"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY82631.1"
                     /translation="MLKLFLDPKMYPYFERYLQRYRNRVFFSDRDLPDPKAEAWEVGV
                     FSKDYFPGALDLQVLQARFPFCSLFFLPHTVVSTDLSHEKIYLYDDESILAHNVSLLL
                     ERILLIRELKESLSRNDFLTHPFTQGPAYREFIQSLQSLRWSPLHLLILGEAGIDKLS
                     LVQYLYPGLCVDRFDFSAIEDPLFPFYMLGDQFSEGFLRKPHQCVFFDNIQDLSEAHQ
                     LFLYSIATQRRYELDDHSGLCDYPILLGAIEDRGDIRRVKELFETVTIPPIRQRKADL
                     AYLITLILNDLAAKYRKEVPPVSIELLLLCQEYPWPENEAQVVRFCELYLLEGVERAT
                     AFLMAGLDAIDTVDKLPKLSALMVSMKDWVETTLIKKAMALTNGNRKQAAPLLGITYK
                     SLCKKLNAPDLKD"
     gene            2932..3216
                     /locus_tag="PKM49_07185"
     CDS             2932..3216
                     /locus_tag="PKM49_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNY82632.1"
                     /translation="MIGAMLTYRYLIKGVVQGVGYRHFVVKMANQLGIKGTVRNLSTG
                     EVEVYASLSEERVRIFENLLQEGPFLSRVEEVLRESAEGVGPFEGFSVLF"
     gene            complement(3257..3925)
                     /locus_tag="PKM49_07190"
     CDS             complement(3257..3925)
                     /locus_tag="PKM49_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY82633.1"
                     /translation="MKKAWIISILLIVSLSFAATAKNVLIESFTATWCGPCRTFDPIV
                     NEMYDADPSVILVHYHVQNDGFDFNWTNNRINWYRNEGIPMFILDGVERKVGGSAAFY
                     MQFQKLVTDRKAASVSNPVDISLTYKDGLVEYTLSPETPLENAQIVVLLIEDQVSDGR
                     SLLRNVVRVAQTTTVKLLENAKKEHVQIPLKPSWRKDKLFSVVFVQKIADREVVGVAQ
                     SHRP"
     gene            complement(4034..4834)
                     /locus_tag="PKM49_07195"
     CDS             complement(4034..4834)
                     /locus_tag="PKM49_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY82634.1"
                     /translation="MKKALSRKQKRAFWKTLTKSAGQLLMFGGLVFRCPMSFFVFPGL
                     NCYAFPWGCTACPMGTLQNFLVFRYIPFFPVASLSLVGLLAGRGWCGWFCPFGTFQDV
                     IGRVKRPGSLPWVKYVVLAVALALAYTFKDPLFCKICPAGTLEGTLPNILIGIATFNP
                     ASILHIVVFLVVVGFTLWMSRFWCRFLCPVGAILGLFNRVTPLQIDYNVNLCVGCDRC
                     SEACPQGLDVPLQVNGTDCIKCGECTVCSALGVSYRLKEKSKPIKKRL"
     gene            complement(4831..5751)
                     /locus_tag="PKM49_07200"
     CDS             complement(4831..5751)
                     /locus_tag="PKM49_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNY82635.1"
                     /translation="MKTQDAMRSNVVSREFRRRLWVLAILALSCSLFAALSFEAEYER
                     TDGLFLVLKIRNSSPLVYYENLSLLLPGLETRVEIEKVEGGQTYVERVGVERTAERID
                     VKVRYTFCEGDVCQYREEEIVIRPERFPKWVRVLGEAAYFAAIALLCVWLWPRTKKAF
                     VFVVILIALPGWVGIRLYQHETVRSIASTLCISCVGLEVVPDSFEITAAYIEEVLKIP
                     YARVEVFHTDWCLSCPVVIDFLEELAMYCDNLELVIIDAQKQPGLAEEKGVYSGNTMV
                     VPTTIVNGKARITGAERFPERFLKSLREVK"
     gene            complement(5748..>6705)
                     /locus_tag="PKM49_07205"
     CDS             complement(5748..>6705)
                     /locus_tag="PKM49_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HNY82636.1"
                     /translation="PAHQVNWSLIGFAAAFITFFLFSKLRLERFWPLYLVIIFASLAA
                     VLRYGEEINSSSRWFETPLGSIQPSELSKPLMVIVLSALFDRLRPGFFFMAIPVGITL
                     GTAFLIFLEPDFGTVFIFLFLLFVLYWMNEEDRGRLFRLVLAGGLLGAFLWFFGLRDY
                     QKARIIAFLYPEKAPDTYYHTQQSITMVGSGGLHGKGFMQGPGNIYGYIPADHTDFVL
                     AVFAEERGFLGLCFFIGIWVILLLAILLEARRKVGFARNCLLGVFSIFFFQTFFNVAM
                     VLGLAPVTGVPLPFFTYGGSSMLANSILIGLTLYAWNKGETR"
BASE COUNT         1721 a         1781 c         1761 g         1442 t
ORIGIN      
        1 agaaagtggc ttttatcgcc gtttgtagca cttttagtcg aaagttgggc tgggaactcc
       61 aacttttatg ttggcaggtt tttttctttt tccaggagag ccctgtccct taccagtttt
      121 tcttctgtgc gctctgagcc ccctttgcga atcctgtttt tttaaaacat gccaggctaa
      181 agtccgccgc tctggcggcg ccccgaaggc gttcccaggc gttccctggc gttctcaaga
      241 atcttttctc tgtgccatct gtgtcctctg tacgaacctt gatttttgat ctttgcaacg
      301 aacgaaaacc gggtatcgcg ccgtcgcgaa aagaagggac gcttacgccg cgatgccctc
      361 acgtaaaagt gtatcgcgcc gtcgcgaaaa gaaggggcgc ttacgccgcg atgccctcac
      421 gtaaaagtgt atcgcgccgt cgcgaaaaga aaaggcgctt acgacgcgat gactttatgg
      481 ttcatcaaag acttttcgac cgatttcccc aagggcagcc actataaaaa gacgcgaggt
      541 tgtggaattt gcacccgctt ttctgataga atagagggct ggtaagtcga gagaagggtt
      601 ttaaaacctt tttctaacca tacgggagga agagcatgcc aaagacgctg gctgtggtag
      661 ccataggcgg aaatgcagtc aataagccga aagaaagcgc gactgcggaa aatatgttcc
      721 gcaacctttc tgttacggcc gagtatttaa tgcagatgtt gaaagacgag tacgagttgg
      781 tcattaccca cggaaacggc ccgcaggtcg gaaatatcct cattcaacag gaagcgggaa
      841 aggagaagat ccctcccttc acgatcgacc ttaacgacgc gcagacccaa gggagtttag
      901 gatacgcgat cgcgttgacg ttgcgcaacg ccttgctgca ggccaatcat cctagagaga
      961 tctgttctgt catcacccaa atcgtcgtcg atccgaagga cccggcattc caaaatccga
     1021 cgaaaccgat cggccctttc tattctgaag aagaagggaa gcgcatacaa gaggaaaagg
     1081 ggtgggtctt cgtagaggac acgggcagag gttggaggcg cgtcgtccca tcccctatcc
     1141 ctttggatat catcgaaaag aaagcgatca agcaactggt caaagcgggg atcatcacca
     1201 tcgcggcggg cggcggaggt attccggtga atcgcttgcc cgacggctca ttacaagggg
     1261 tggaagccgt tatcgataag gaccgggcct ccgccttgct cgccttagag ctcaacgcag
     1321 atatgttcat catcctcacg ggcgtcgaaa aggtctattc ggactatggc aagcccacgc
     1381 agaaagcctt cgataccctc acggtggagg aggcgaaccg ctaccttcac gggggccaat
     1441 tcccgaaagg cagtatggga ccgaagatcg aagcggcgat agcctatgtg tccaagacgg
     1501 ggaaaaggtg tttgatcacc gcgatggaaa cgttaggcga cgcgctgaaa ggaaaaaccg
     1561 gcacgtggat cgttaattga cggatccgcg cgtgaccggg aagggcaagc cgccgttcgc
     1621 ttcaagttcg ttgtaagagt gattcttcgg tcgatatagg cggagccagt cggaaaacga
     1681 gagcttgaag ccttagatcg gagttggggg aagcgaaatg ctaaaactat ttttagatcc
     1741 aaaaatgtac ccgtatttcg aacggtacct tcagcgttac cgtaaccgcg tcttcttttc
     1801 cgacagagac ctcccagacc ccaaagcaga ggcgtgggag gtcggcgttt tctcaaaaga
     1861 ttatttccca ggcgcactcg atttacaggt attacaagcg cgctttccct tttgctctct
     1921 ctttttttta cctcacaccg tcgtctcaac ggacctgtct catgaaaaga tataccttta
     1981 cgatgatgag agtatcttgg cccacaacgt ctctctactc ctggaacgca tcctcctcat
     2041 acgggagctg aaagagagcc tatcgcgtaa tgactttttg acacaccctt tcacacaggg
     2101 cccggcctat cgcgagttca tccagtcgct ccaatccctg cggtggtccc ctttacacct
     2161 tctgattttg ggagaagccg ggatcgacaa actctccctc gtccaatatc tctaccccgg
     2221 cctatgcgtc gacagattcg atttttccgc gatagaggac cctctgtttc cattctatat
     2281 gcttggcgac caattttcgg agggcttttt gagaaaaccc catcaatgcg tcttcttcga
     2341 caatatccaa gacctatccg aagcgcatca gctgttcctg tactcgatag cgacccaacg
     2401 gcgctatgag ctcgacgacc actccggttt atgcgattac cccatcctct tgggggcgat
     2461 agaggatagg ggggacatcc gccgggtcaa agaactcttc gaaacggtga cgattccgcc
     2521 tatacggcag cggaaggccg atttggccta tttgatcacg ctaatcttga acgacctcgc
     2581 cgcgaagtac agaaaggagg ttccgcccgt cagtatcgag ctcctcctgc tctgccaaga
     2641 atacccgtgg ccggagaacg aagctcaggt cgtccgattt tgcgagctct atctcctgga
     2701 gggcgttgaa cgagcgaccg cctttttaat ggcgggattg gacgcgatag acaccgtcga
     2761 caagctcccg aaacttagcg cgctgatggt gtctatgaaa gattgggtgg aaaccacgtt
     2821 gatcaaaaag gcgatggctc tgacgaacgg aaaccgcaaa caagccgccc cgctcttggg
     2881 aatcacctac aaatccttgt gtaaaaagct gaacgcgccg gatctgaagg attgatcggc
     2941 gcgatgctca cttaccgata cttaatcaag ggcgtcgttc aaggggtcgg ataccgtcat
     3001 ttcgtcgtga aaatggcgaa ccagctgggc atcaagggaa ctgtccgtaa cctgtccacc
     3061 ggcgaagtag aggtttacgc gagcctctcc gaggagaggg tgagaatatt cgaaaacctc
     3121 ctccaagagg gcccgttttt gtcccgggta gaagaggtct tacgggagag cgcggaaggg
     3181 gtcggccctt tcgagggctt ctcagtcctc ttctaaaggc gagacccaat tccgactgcc
     3241 cttaaccctc tgcgtcttag ggccgatggg attgggcgac tcccacaacc tctcgatcgg
     3301 ctatcttctg gacgaataca acggagaaga gcttgtcctt ccgccacgag ggcttcagcg
     3361 gaatttgcac gtgctctttc tttgcatttt ccaataactt tactgtggtc gtttgcgcaa
     3421 cgcggacaac attgcgaaga agcgaacgcc cgtccgacac ctggtcttct atgaggagaa
     3481 cgacgatttg agcgttttca aggggtgttt cggggctaag agtatattca acaagcccat
     3541 ccttgtaggt cagcgaaatg tcgaccggat tagaaacgct cgcggctttc ctatctgtga
     3601 cgagcttttg gaattgcata taaaaagcgg cggaacctcc gacctttctt tccacaccat
     3661 ccagaatgaa catcggaatt ccctcattcc tataccagtt gattcggttg ttcgtccaat
     3721 tgaaatcgaa accgtcattt tgcacatgat aatgtactaa gatcacagac gggtcagcat
     3781 cgtacatctc attgacgatc ggatcgaaag tcctgcacgg accgcaccaa gtcgccgtaa
     3841 aagattcgat cagtacgttc ttcgcggttg cggcgaagga gagagacacg atcaagagta
     3901 tggagataat ccaagctttt ttcatagaac ctccttgcgg tttattgatg atatcggacg
     3961 gcgaattcac ctttcatatc gccttttcta gcgtctcctc acaaggttgg aggcgtatga
     4021 ggatcagctc tgactataga cgttttttta taggtttgga tttttctttc agtcgatagg
     4081 aaacacccaa agcagaacaa accgtacact cgccgcattt gatgcagtcc gttccgttta
     4141 cctgaagcgg aacgtccagc ccttgcggac acgcttcgga gcagcggtcg cacccgacgc
     4201 aaagattcac attgtaatct atttggaggg gagtcacacg attgaacaag ccgagtatcg
     4261 ccccgaccgg acacaagaac cggcaccaga accggctcat ccacaaggtg aagcctacga
     4321 ccacgagaaa gacgacaata tggaggatcg acgcgggatt aaacgtggcg atgccgatca
     4381 agatattcgg cagcgtgcct tcgagagtac ctgccggaca gatcttgcaa aatagtgggt
     4441 ctttgaaagt ataggccaaa gccaaggcaa cggccaagac gacgtacttg acccagggga
     4501 gtgagccggg tcttttcacc cgaccgatga cgtcttgaaa tgtgccaaac ggacagaacc
     4561 acccgcacca cccacggccg gccagcagcc cgacgaggga cagggaagcg acggggaaga
     4621 aagggatgta ccgaaaaact aaaaagttct ggagcgtccc catcggacag gccgtgcatc
     4681 cccaaggaaa ggcgtaacag ttcagcccgg gaaagacaaa aaaggacatc gggcatcgga
     4741 agacgagacc gccaaacatc aacaactgac cggcgctttt cgtgagcgtc ttccaaaagg
     4801 cgcgtttttg ttttctggat aaggctttct tcatttgacc tccctcaagc tcttcaagaa
     4861 tcgttcaggg aaacgctcgg cgccggtgat acgcgctttt ccgttgacga ttgtggtcgg
     4921 aaccaccatc gtgttccccg aatacacacc cttctcctcg gccagccccg gctgtttttg
     4981 cgcgtcgatg atgacgagct ccaagttatc acagtacatc gccaactctt ccaagaaatc
     5041 gatcacaacg ggacaggaga gacaccaatc cgtatggaaa acttcaaccc gtgcgtacgg
     5101 gatttttaaa acttcctcga tataggcggc cgtgatctcg aatgagtcgg ggacgacttc
     5161 caggccgaca caggagatgc agagggtcga tgcgatcgaa cgcacggttt cgtgttgata
     5221 cagacgtatg ccgacccacc cgggcagggc gatgaggatg acgacgaaaa cgaaggcttt
     5281 tttcgtgcgt ggccagagcc agacacatag gagagcaatc gcggcgaagt acgcggcctc
     5341 accgagtacg cgcacccatt ttgggaagcg ctcggggcga atcacgatct cctcttcccg
     5401 atattgacaa acgtcgcctt cgcagaaggt gtacctgacc ttcacgtcaa tacgctcggc
     5461 tgtccgttcg acgcccaccc gttcgacgta ggtctgaccg ccttccacct tctcgatctc
     5521 cacgcgcgtc tcgagaccgg gcaacagaag ggaaaggttc tcgtaataaa cgagaggaga
     5581 agaattccgg attttcagca ctaaaaataa cccgtcggtg cgttcgtact ccgcctcgaa
     5641 cgaaagggca gcgaaaaggg agcatgaaag cgcgaggata gcgagaaccc ataacctgcg
     5701 cctgaattct cgactcacga cgtttgaacg catcgcatct tgtgttttca tcgcgtttct
     5761 cctttattcc aggcatagag cgtcagcccg attaaaatcg agttcgccaa catcgagctg
     5821 ccgccatagg tgaaaaacgg gagggggacc ccggtcacgg gcgcgaggcc caagaccatc
     5881 gccacattaa aaaaggtttg gaaaaagaag atcgaaaaga cgccgagcaa gcaattccgt
     5941 gcgaaaccca ctttgcgccg cgcctccaat agaatcgcca gcaggagaat cacccagatt
     6001 ccgatgaaga aacagagccc taaaaaaccg cgctcctccg cgaaaactgc caagacgaag
     6061 tcggtgtgat cggctgggat atatccgtag atgttccccg gcccctgcat gaatcctttt
     6121 ccgtgcaggc ctcccgaacc gaccatcgtg atcgactgtt gcgtgtggta ataggtgtcg
     6181 ggggctttct ccggatacaa gaaggcgata atccgcgctt tctgataatc gcgaagaccg
     6241 aaaaaccaca gaaaagcgcc taagaggcct cccgctagaa ccagccggaa aagccgaccc
     6301 cgatcttcct cgttcatcca gtataaaacg aaaagcagaa agaggaagat gaacacggta
     6361 ccgaaatccg gttccaaaaa gatcaggaag gcggtcccta gggtgatgcc cacggggatc
     6421 gccataaaaa agaacccggg tcgcaacctg tcgaaaagcg cggatagaac gatcaccatc
     6481 aacggtttgg agagttcgga gggttggata ctccctaaag gagtttcaaa ccagcgagag
     6541 gatgagttga tctcctctcc ataacgcagg acggcggcga gactcgcgaa gatgatgact
     6601 aaatagaggg gccaaaaccg ttccaagcga agcttggaaa agagaaaaaa ggtgatgaaa
     6661 gccgccgcga aaccgatgag gctccaattc acctgatgcg cgggc
//
LOCUS       DAOPXN010000121         6636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11508_length_6636_cov_5.438231, whole genome shotgun sequence.
ACCESSION   DAOPXN010000121 DAOPXN010000000
VERSION     DAOPXN010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11508_length_6636_cov_5.438231"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1003..1554
                     /locus_tag="PKM49_07210"
     CDS             1003..1554
                     /locus_tag="PKM49_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNY82637.1"
                     /translation="MDREEAWALLNEYTQKDGLLKHALEVEAGMRAYAERYGENADVW
                     GIVGLLHDFDYERYPTEEDHPFRGAEILKEKGLPQDWIDAILGHADYTGVPRETRMAK
                     ALYAVDELTGLIHAAALVRPSKKIEEVEVKSIRKKMKDKAFAANVSRADIENGAKDLG
                     LELDDHIAVVLNAMKGIAGDIGL"
     gene            1557..2102
                     /locus_tag="PKM49_07215"
     CDS             1557..2102
                     /locus_tag="PKM49_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82638.1"
                     /translation="MVTAAPFLVPKNPYSDKANTLYENRRIGFLLSLLKTEDKKAVFL
                     EKIQQLEQYRKAGHRALFMDLFAQIEDQLLGQSDEAAAEAFAFSFFDGGFERTKAPRT
                     VAYQTITLSSIFSFGEEGEKKRQSIRETVFGKAPLDRYFQYLSGIGPVGMIDRINNHL
                     VSRSVDRGYESMKGYWILAYA"
     gene            2338..2595
                     /locus_tag="PKM49_07220"
     CDS             2338..2595
                     /locus_tag="PKM49_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HNY82639.1"
                     /translation="MIAFIAGLWIGYKRRPAFFKKYKVVLWITLMLLFLMGYETGSNA
                     ELFEALPRIGWWALVIAVCGALGGFCFVFLFERVLKKRKSL"
     gene            2592..3176
                     /locus_tag="PKM49_07225"
     CDS             2592..3176
                     /locus_tag="PKM49_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HNY82640.1"
                     /translation="MILMLSAVTCGILLGRFFTLPVPDFILTVLLMALVFFVGLEIGS
                     EEGIFKKIKKELALILIQSALVISGTLVFGWLACLLIPSSLFSGKETMGAAAGMGWYS
                     LSGVMISEMHSPLLGTVSFTANLIRETLAILLIPVLSRFSPLASVSIGGAAAMDVLLG
                     LISKHNRLSVTLIAFGQGVICSLMVPVILTIIFG"
     gene            3346..4266
                     /locus_tag="PKM49_07230"
     CDS             3346..4266
                     /locus_tag="PKM49_07230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNY82641.1"
                     /translation="MVLEEGINQDFEFYFRLLKDLADGICIANDHMKIVFWNAPMVQN
                     LDYSTTEAWGQSLVDLLQLRSAHNEENHFLEEDIRRMAFEGVVTTVFCRHRAGHSVAM
                     QVRINTFTDPESGCRGFVLIFCDALAKGTIEKGVMELKEIAYFDQLTGLPNRRYLEIE
                     MEKHLSSFRRYHIPFGVLLIDIDFFKKVNDTYGHDNGDRVLQALGQTLLTETRKDDVK
                     TRWGGEEFVLLTRLSDPDKLIAFGERLRAIIEKAPVEIPESGITIHFTISIGVTLSRP
                     GDDRNTLLKRCDDMLYKSKKEGRNRVSGLF"
     gene            complement(4267..4587)
                     /locus_tag="PKM49_07235"
     CDS             complement(4267..4587)
                     /locus_tag="PKM49_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HNY82642.1"
                     /translation="MIVTTTPSIEGKTIVEYKGIVCGEVISGINFVKDFLAGLRDVFG
                     GRAGAYEGELIAARESALNEMVDRARSMGANAVVGVDIDYEVIGQNGSMLMISASGTA
                     VVVR"
     gene            complement(5093..6283)
                     /locus_tag="PKM49_07240"
     CDS             complement(5093..6283)
                     /locus_tag="PKM49_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019536903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNY82643.1"
                     /translation="MSDALRGLLEHAFINRALISGTFSSPKGNLDDLAKKITVKPIKT
                     KAGYVFQFEKFYPTRVLHENLKPPVAFEKALALVRDSYRQTVLFLPEADYHILSRGGE
                     AKIIKRQASRSEATLSRDRPKNYRLPKGQAVDYLVRLGIMNPEGQVFQKKYDKFRQIN
                     KFVEFVEDALNKAELSEQHLRVIDFGCGKSYLTFALYHYLTNVRGLSVEATGLDIKED
                     VIDLCNRLADELGYRTLRFAVGDIASYDFAEKAHMVVTLHACDTATDEALLRAIRWQS
                     DVILSVPCCQHELFPKIHHWELKPLLKHGVMKEKLASMVTDAIRACLLETFGYEVSVM
                     EFIDMEHTPKNVLIRALLRKPGFYPEKYKVFKDFLETWGLSETYLEKGVVAHRLIPEF
                     YGTR"
     gene            complement(6351..>6636)
                     /locus_tag="PKM49_07245"
     CDS             complement(6351..>6636)
                     /locus_tag="PKM49_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNY82644.1"
                     /translation="LGASLVRVSRQGNGFEGLQEEFAYERWVSAWSESGIDPHRYTGQ
                     REIDGVFPWDVVDAGLTKAFLRSEYEKALRGEPTPDCRMGACNQCGVCDP"
BASE COUNT         1538 a         1668 c         1806 g         1624 t
ORIGIN      
        1 tcacacagag aacacggagg gcacggagag cgcagagcgg agattcgcgc tgggaacgtc
       61 gtgggaacgc cgttggaatg tcgtcggagc accgaactgg gaacgccaac ttttaagttg
      121 gcatgtcttc ataagtgtct tgtgaaacca acaaaagcct tcgctcacgg agaacgcgga
      181 gtcaagatga aaaacgcgat gaacgaaaag attaacgtgc gttttctcag agcttctcga
      241 aagcggtccc agaaagacac gccgaactaa agttcggcgt tcctaggaag gcgttctcag
      301 caaaaaacct taaaacatgc cgaactaaag ttcggcgttc ccaggcgctc ccaaggtgcg
      361 gcattcccgg aacgtcgttc ccagaaaggc gcgctcagcc gtatacctct accatcaagc
      421 tgtgtgggtc aagttatggg gtgccgccaa gaaacaaggc cagaatcgaa cgcagggttt
      481 ttagtcaatg ttgtcatcgc ggcgtaagcg cccattctct tcgtggggcc gacttccagt
      541 cgcctgtctc tctgggaagg ccttgggaac accgaacttt agtccgaaat gggtacctgg
      601 gaacgccgaa ctttagttcg gcatgttcta ttgttctatc aatgtccgat tttttaggag
      661 taaaatcaga cgtaggctca agagaagtcg ccatgaaaaa agagggggtc actcggcttc
      721 gtaaaaaagg catagggcgg atcgcggggt cgtaaaaacc acttaccccg cgataacctc
      781 acaggcccat cgcagcgtaa gcgtttaagg gctctcgaga gcttacagaa atgcaaagaa
      841 acatgccgaa ctaaagttcg gcgttcccat ttcggtgcac cccgatatta tacccatacc
      901 atcaggcttg acgtcgctgg gcttcgtgct ataattgatg gaactgtgtt tttaccgtcg
      961 ttgttcgaag cagcacgtag gaatgattcg ggagggagct cgatggatcg agaagaagct
     1021 tgggcgctat tgaatgagta tactcaaaaa gatgggttgc ttaagcacgc gttggaagtg
     1081 gaagcgggga tgagggccta tgcggagcga tatggagaga atgcggatgt gtggggaatc
     1141 gttgggcttt tgcacgactt cgattacgag cgctacccga ctgaggagga ccacccgttt
     1201 cggggcgccg agatactaaa agaaaaggga ttgccccaag attggatcga tgcgattctc
     1261 ggacacgcgg actataccgg agtacccagg gaaacacgga tggccaaagc gctttacgcc
     1321 gtcgacgaac ttaccggctt gatccacgcc gcggcgttgg ttcgtccctc aaaaaagatc
     1381 gaagaggtcg aggtgaaatc gatcaggaaa aagatgaaag ataaagcctt cgccgcgaac
     1441 gtcagccgcg cggatatcga aaacggcgcc aaagatttgg gcctagagct ggatgaccac
     1501 atagcggtcg ttttaaacgc tatgaaggga atcgcaggag atatcgggtt ataactatgg
     1561 tgaccgccgc cccttttctc gtaccgaaga acccgtattc ggacaaagcg aatactttgt
     1621 acgaaaaccg caggatcggt tttcttcttt ctttgttgaa aacggaggat aaaaaggcgg
     1681 tctttttgga gaaaatccaa cagttggagc aataccgaaa agcggggcat cgagctctct
     1741 ttatggatct tttcgcccaa atcgaggacc aacttttggg ccagagcgat gaagcggcag
     1801 cagaggcatt cgccttttcc ttctttgacg gcggatttga gcgaacgaag gccccaagga
     1861 ctgtggctta tcaaaccata acgctatcgt caatcttttc tttcggagaa gagggagaaa
     1921 aaaagcggca gtcgatccgt gaaacggtct tcggcaaagc tcccctcgat cgctactttc
     1981 aatacctttc cggtatcggc cctgtcggga tgatcgatcg gatcaacaac catctcgtct
     2041 ctcgttctgt tgatcgcgga tacgagagta tgaaagggta ctggatactc gcttacgcgt
     2101 aacccgcccc aacttcgcgc tcggagacaa gaaccatacc agaaaagcgc tccgatcagc
     2161 actcggtaag cttggcatcg tcactgctgt gcgacgtctc catcccccca ttcgggaagg
     2221 ccgttttatg atataatccc gctgcacggg cggcagggtg cccgccaaaa gggtagaaag
     2281 cagaaaatag ggacgacaga tgaaagcggg tgttcaaacg tggtcttcgc tgggattttg
     2341 atcgcattca ttgccggttt gtggatcggc tataaaaggc gtccggcgtt tttcaaaaag
     2401 tacaaggtcg ttctttggat cactttgatg ctcctgtttc tcatgggcta cgaaaccggt
     2461 tcgaacgccg agctgttcga agccctgccc cgtatcggct ggtgggcttt ggtcatcgcc
     2521 gtatgcggcg cccttggagg attctgtttc gtgttcttgt ttgaacgggt tttaaagaag
     2581 aggaagtccc tatgatccta atgctctccg ccgttacatg cggcatcctg ctcgggagat
     2641 ttttcactct tccggttccg gactttattt taacggtttt attgatggcg ttggtgttct
     2701 ttgtcggctt ggagatcggc agcgaagaag gcatatttaa aaaaatcaag aaagaactcg
     2761 cgctgatctt gattcagagc gccctcgtta tctccggaac attggttttc gggtggctcg
     2821 cctgtctctt gatcccctct tctctgttct cggggaaaga gacgatggga gcggccgcgg
     2881 ggatggggtg gtatagcctg tcgggcgtga tgatctcaga gatgcattct ccgttgctag
     2941 ggaccgtttc atttacggcg aatctcatac gagaaacgtt ggccatactg ttgattccgg
     3001 ttttgtctcg attttctcct ctggcgtccg tctcgatcgg cggcgcggcc gctatggatg
     3061 tgctgctcgg gctgatctca aagcataacc gcttgtcggt gacgctgatc gccttcggcc
     3121 aaggggtgat ctgttcgttg atggtgccgg tgattcttac gattattttc ggataactct
     3181 ttctcgctag ggcccggatc aggttaccca aacgggtagg gccgatttga cccgctgtta
     3241 acgattggaa gaggcgttaa gcgtaccggg cacaagggat actgatcccc ccggtgaggg
     3301 ctagaacgcc cataacgcgg acaaacttag gagggcggag aatctttggt tctcgaagaa
     3361 ggcattaatc aggatttcga gttttatttc cgtttgctca aagacctggc ggacggtatc
     3421 tgcatcgcta atgaccatat gaagatcgtc ttttggaacg ctccgatggt ccagaacctg
     3481 gactattcta caacggaggc ttggggccag tcgttggtcg atcttttgca gctccgcagc
     3541 gcgcataatg aagagaacca tttcttggag gaggatatcc gccggatggc tttcgagggc
     3601 gtggtgacga ccgttttttg ccgccatcgc gcgggccaca gcgtggcgat gcaggtgcgg
     3661 atcaatacct ttacggatcc ggagagcggt tgtcgtggat tcgtcctaat tttctgcgat
     3721 gcgctggcga aagggacgat cgaaaaaggc gttatggaat tgaaggagat tgcctatttc
     3781 gatcaactga caggattacc gaatcgccgg tatttggaaa tagagatgga aaaacacctc
     3841 agctcttttc gccgatacca cattcctttc ggagttttat tgatcgatat cgactttttt
     3901 aaaaaggtca acgataccta tgggcacgac aacggggata gggtcctcca agcgctcggc
     3961 caaacgctcc tcacggaaac ccgaaaagac gacgtgaaga cccgatgggg cggtgaagaa
     4021 ttcgtcctgc ttacgaggct ctctgatccg gataaactga tcgctttcgg agaaaggtta
     4081 cgagcaatca tcgagaaagc ccccgtcgag attccggaga gcggtatcac gatacatttt
     4141 acgatctcta tcggagtcac gttgtctcgg ccgggagacg atcgaaatac cctattaaaa
     4201 cgctgcgatg acatgcttta caagagtaaa aaagaagggc gtaaccgagt ttcgggcctc
     4261 ttttaattat cgaacgacga cggcagttcc ggaggccgag atcatcagca tactcccgtt
     4321 ctgcccgatg acctcgtaat cgatatcgac ccccacaacc gcgttagcgc ccatcgacct
     4381 cgctcggtcg accatttcgt tgagcgcgct ttccctcgcc gctatcaact ccccttcgta
     4441 ggctccggct cgccctccga agacgtcccg gagcccggcc aagaagtcct tcacgaaatt
     4501 gatgccggat atcacttctc cacacacgat tcctttgtac tcaacgatag tttttccctc
     4561 gatcgagggg gtggttgtca cgatcacggt cattcctcct tcaaagaata ataggtactt
     4621 atcctgatgc gcgcacgtag cagtagtgcc gataggcgcg ggatatcgga atacctctta
     4681 tgtttttacg ctttcgggtc gcaattgaca gttggataaa gagaggttcc gcttcgtgta
     4741 tataaaaagc gatgcgtcgg acttgcgtct agcaaggttt tctgggaacg ccgagcttta
     4801 gttggcgtgt ctctacgtgt tccttcggcg caagaaaaaa cggaacatat cgattgtgag
     4861 gggagtattc taggagccgt tttccgtagg gctacgaagc atctccgtaa gctcctcgtc
     4921 ttctgcgcgc aaagggtatt atcggtttag cctcgtcgta ggaggtagag caccgagttc
     4981 ccttatgccc gacttctctc tttcttttca acgaccgatt tctttgactt tttcgctcca
     5041 cctgttttag taaggcgcac gcgcgcggtg agtaggctct tttagcgcta cgctaacgcg
     5101 tcccgtagaa ttccggaatc aagcggtgcg ccactacccc tttctctagg taggtctccg
     5161 agagccccca cgtttctaaa aagtctttaa agactttata cttttcgggg taaaaccccg
     5221 gttttctcaa caaagcgcgg atcaggacat ttttaggggt gtgctccata tcgatgaact
     5281 ccatcacaga cacttcgtaa ccgaaggtct ctaaaaggca ggcgcgtata gcgtccgtga
     5341 ccatggacgc caacttttct ttcataacgc cgtgtttaag cagcggcttc agttcccagt
     5401 ggtgtatctt cgggaaaagt tcgtgttggc agcacgggac actgagaatc acgtcgctct
     5461 gccatcgtat cgcccgcaac agcgcttcgt cagtggcggt gtcgcaggcg tgcaacgtga
     5521 cgaccatatg ggctttttcg gcgaagtcgt aggacgcgat gtcccctacg gcgaagcgta
     5581 acgtgcggta tcccaattcg tcggccaagc ggttacagag atcgatgacg tcttccttga
     5641 tgtccagtcc cgtcgcttct acgctcaacc cgcgcacgtt cgtcaaatag tgatacagag
     5701 cgaaggttag gtaggatttt ccacagccga aatcgatgac gcgaaggtgt tgctccgata
     5761 actcggcttt gttgagggcg tcttcgacga attctacgaa cttattgatt tgccggaact
     5821 tgtcatactt cttctgaaag acctggcctt ccgggttcat aatgcccaat cgaacgagat
     5881 agtccaccgc ctggcctttt ggcaaacggt agtttttggg ccggtcgcgc gagagcgtcg
     5941 cttcagagcg gctggcctgt cgtttgatga tttttgcttc gccgccccta gagagaatgt
     6001 gatagtcggc ttccggtaaa aagagaacgg tctgccgata ggagtcccgt acgagggcga
     6061 gggccttttc gaaggcgaca ggcggtttca ggttttcgtg caaaacgcgc gtcgggtaga
     6121 acttttcaaa ttgaaagacg tatcccgctt ttgttttgat cggcttaacc gttatcttct
     6181 tcgcgagatc gtctaaatta cctttagggg agctgaacgt gccggagatg agcgcgcggt
     6241 ttataaaagc gtgttccagc aaccctctca aagcgtctga cataggaccc cctcctgcgc
     6301 tctaggacgg agttcgaagc cggcctttcg cccacccgct ttttccaacg ctacggatcg
     6361 catacgccac attggttgca cgcgcccata cgacagtcgg gggttggttc tccgcggagg
     6421 gctttttcgt actcggatcg tagaaaggct ttggttaaac cggcgtccac gacatcccaa
     6481 gggaaaaccc cgtcgatctc gcgctgcccg gtgtatcgat ggggatcgat tcccgattcg
     6541 ctccaggcgg atacccaccg ttcgtatgcg aactcttcct gtagaccttc gaatccgttt
     6601 ccttgacgag acacccgcac taaagaagcc cccagt
//
LOCUS       DAOPXN010000122         6608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11564_length_6608_cov_4.641691, whole genome shotgun sequence.
ACCESSION   DAOPXN010000122 DAOPXN010000000
VERSION     DAOPXN010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11564_length_6608_cov_4.641691"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..911)
                     /locus_tag="PKM49_07250"
     CDS             complement(132..911)
                     /locus_tag="PKM49_07250"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HNY82645.1"
                     /translation="MRGRFADTIDINCDAGESFGVFSSGEDEGLFPYVSSVNIACGFH
                     AGDPKVLDKTVGLAERYGLQIGAHPGFFDLRGFGRRELSLPADEIRTDVLYQLGALDA
                     FLRVRGLRLAHVKPHGALYNMAAREEGIAIPIVEAVKAYDPSLVIVGLSGSALERVTT
                     EQGLRFAREAFLDRQIHSDGSLVSRRDPNAFVPEKELVPRAIRLIRKGEVVSVEGALV
                     PCVCDTLCLHSDTPGAARFAKVLQEALIAAGISVEPFRTED"
     gene            1111..1815
                     /locus_tag="PKM49_07255"
     CDS             1111..1815
                     /locus_tag="PKM49_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82646.1"
                     /translation="MRIQAYEIEKKYRFFSLPLAEQFFRQPNFLWRKIGILQWYLNEK
                     EDTDLSIRIRLTLKKDEEGFKEEWEIGSKRPVPHHPEQRVETQTSFSFSECPSDVALT
                     LEEFGVKLPGDLDRYPVLAKIRYIMVKPLKIVRVAEVVLDHFIYPIMKRGERVHLELE
                     LRDKTPLPGQAEQFESLFGRLNLYTFARDITSKTYLSNRSLAERAFEEGKCGFGIEMG
                     RMEIERLLRAKEPFGR"
     gene            complement(1840..2385)
                     /locus_tag="PKM49_07260"
     CDS             complement(1840..2385)
                     /locus_tag="PKM49_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNY82647.1"
                     /translation="MVLAAGEGKRFGGNKLAAVLPDTGRTVLEQVIDKALLRQRRADS
                     LDSWRVLVVSNPINARTIDASREVEVFVNERYREGLSESVKVGIREAIRRKVSGVFLF
                     LGDMPFISETTVESVYQKANQEPEAIIRPRYQNTPGFPLYFPARFFDRVWRSPGIAGS
                     KLFWGLCHSPCGGLKPRTLSA"
     gene            complement(2399..4600)
                     /locus_tag="PKM49_07265"
     CDS             complement(2399..4600)
                     /locus_tag="PKM49_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNY82648.1"
                     /translation="MRKYLFWLLALITLSALLSANGVMIPYSERIDLQPVVVFPRNPV
                     SMLEHLVHIDINEDIAKFSIREVFYNESNAKVEVVYLFPLPDQTVITDFKMAVNGVVY
                     EGTIMEKDEARLLYESYVRQQRDPALLEYIGQRMIRIRLFPFAPFEKREIEVEYYQEV
                     DKTGDYHKLVYPLKIDSLLKGNIGRIKIEGVIHSQRLVYTVNSPTHTLAFTPQPQEAV
                     SFVYAEENLFPSQDFVLFIGYGERRYEAYFVNDEEEPEIGTFMLDILPYYPDVGTLQK
                     SVMVVLDKSGSMYGTKYAQALAAAQFILERLNQEDRFGLLLFNDKMTPFRKELLTTLY
                     DQKDTPDALKWLKGSSAGGTTDIHEALRLGTLSAFTNHEDENNYVIFLTDGQPTSGIL
                     NISKITSDAIGWAKATGAKLFVFGVGYDVNTGLLDLLAQESGGMAFYVEEKENIEAKV
                     SQLFANVSNPLMTDIQVEIAGENAQITDLLPSRNLTIYKDFPLKLFGRYTGHGELQIR
                     ITGKIAALDGSGVIAFEQIYPVTLSEHSNPYISKLWASRRISELLNHIKLNGETVEAR
                     EEIVRLSKKYGIPTPYTSYLILEGVTGQDKDIESVDGTVPKILFDMRAFSSAAPAPTL
                     SQTGQGAFEASKMQNLMVQSMTTEELEKAYGTGASAKGYRNLYGKVFLFDEAENAWVD
                     TDFREEGVVEVKAFSDEYFDLIRRLPELKHYLGLGTNFKIQLEGINYRFKE"
     gene            complement(4680..5594)
                     /locus_tag="PKM49_07270"
     CDS             complement(4680..5594)
                     /locus_tag="PKM49_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HNY82649.1"
                     /translation="MGLRTAGILLLTLSIFSFTVSFSAILTVGGGASDYRTIKQAVFA
                     ASSGDTILIRAGTYVENIRFQKALNLKGEGRGRVVLRPADTLIPTIWVENCPALTIEG
                     MTIHGGAIAVSLAMSNARILENTIQAGQDGIRAVTFNHSLFFGNNILYGPFLKSKNAS
                     LSSHGVLSLGIGETVIQYNEVRGFATGLFLTGKKPCRVEKNLFSENLKACFLGGDTVI
                     ELLSNSFKNNSVDGLVLSGKVDCQMKYNVFESNMQWDIRLALSECAVDTRVAFMGTVS
                     GEQNVFDAHYRLCPADYAWGNAFFGPKE"
     gene            complement(5606..>6608)
                     /locus_tag="PKM49_07275"
     CDS             complement(5606..>6608)
                     /locus_tag="PKM49_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNY82650.1"
                     /translation="VALVGVIILLVGKRWTAKRLRSIGKKADAPFFTFLSTILVTTTL
                     PVFYYLVFIIALTPIDMPQSWRDIVNAIGSILLTVAIIRFVTATIVFGVQNYARKHPE
                     NTAQIQGLKSLTSIVKIVAWTLGIVGVMKSLGYDINGILTGLGITGIAVALASQKILG
                     DLFGYFSILLDKPFEPGDFIVFGEYSGTIEKIGLRTTRLRSLTGEQLVIANSLLTESS
                     IRNYKRMVQRRALFQLSLPFDTPLETLKAIPGIVQEIVDRNPDLEFSRAHLSSIGTHS
                     FIYDIVYWVDKRDYMAYMDAQQAINFGIIEAFRDKGIVFAFPTQKVITPSSAPKSIT"
BASE COUNT         1519 a         1706 c         1773 g         1610 t
ORIGIN      
        1 gcagagcgta tcgcgagact tcgtgagctt ccgaagtcag cccgctggtt accccacgat
       61 gaacctctta gttatgtcac cccttaactt gacccacaca gcggatacac gtccggttgg
      121 gtatgtgttc ctcagtcctc ggttcggaac ggttcgaccg agatacctgc ggcgatcaga
      181 gcctcctgca agacttttgc aaagcgggcg gcgccggggg tatccgagtg aagacacaag
      241 gtgtcgcata cgcaaggtac gagcgcgccc tcgacgctga cgacctcccc tttacggatc
      301 aggcgaatgg cgcggggcac gagttctttt tccggaacga aggcgttggg gtctctgcgg
      361 gagaccaagc tgccgtcgga atggatctgc cggtcgagga aggcttcccg tgcgaagcgg
      421 agtccttgtt cggtcgtcac tcgttctaac gctgaccccg aaaggccgac gatcaccaaa
      481 gagggatcgt aggcctttac ggcctcgacg atggggatag cgatgccctc ctcgcgcgcg
      541 gccatattgt atagcgctcc gtgaggtttc acgtgagcta ggcgtaggcc tctaacccgc
      601 aggaaggcgt ccagagctcc cagttggtaa aggacatcgg ttcggatttc gtctgcgggc
      661 agcgaaagct ctcggcgtcc aaaacctcgg agatcgaaaa accccgggtg cgcccctatt
      721 tgaaggccgt atctttccgc cagacccacg gttttatcca ggactttggg gtcaccggcg
      781 tgaaacccgc acgcgatgtt gaccgaggac acgtaaggga aaagcccctc atcctctcct
      841 gacgaaaaga caccgaaact ctctcccgca tcgcagttga tatcgatcgt atccgcaaac
      901 cgccctctca tccgaccgcc tcccgtatgc cacttttttt gttgtataat catatcacac
      961 gagcgtgcga ctccgttttg gatgtgtgag gaagcgaaac cgtgattttt tttcgcaatg
     1021 gaatcccttg aatagaccca ttataatgtc tctcttgaag tcgaaggttg taaaaagcgt
     1081 gacgggtgtt ttggaaggcg gacgatagga atgagaattc aagcctatga aatcgagaaa
     1141 aagtaccgat ttttttctct accccttgcg gagcagttct ttcgacaacc caactttctt
     1201 tggcggaaga tcggcatcct ccagtggtat ttgaacgaaa aagaagatac tgacctatcg
     1261 atacgtatcc gcttgacgct aaagaaggac gaggaagggt ttaaagagga gtgggagatc
     1321 gggagtaaaa ggcctgtccc tcatcaccct gagcagcgag tagaaacgca aacgtctttc
     1381 tctttcagcg agtgccctag cgatgtggcc ttaaccttgg aggagtttgg agtgaagttg
     1441 cctggcgacc tcgatcgata cccggtcttg gcgaaaatac ggtatattat ggtcaaaccc
     1501 cttaagatcg tgcgcgtggc ggaggtcgtc ttggatcact ttatttaccc gataatgaag
     1561 agaggggagc gcgtacacct cgagctcgaa ctgagagaca aaactccctt acctggtcaa
     1621 gcggaacagt ttgagagtct cttcgggcga ctcaatctct acactttcgc tcgtgatatc
     1681 accagtaaaa cttacctgtc aaaccggagc ttggcagaac gcgctttcga agagggaaaa
     1741 tgtggattcg gtatcgaaat ggggcggatg gagatagagc gtcttctcag agcaaaggag
     1801 ccttttggcc gctgaggtcg gaggggaggt cgatatcctt cacgcagaaa gggtcctcgg
     1861 tttcaaccca ccgcaaggac tgtgacaaag gccccaaaac agtttggagc ccgcgatccc
     1921 cggtgatcgc cataccctgt cgaaaaaccg tgcggggaag tagagcggga accccggtgt
     1981 gttttggtaa cgtggccgta tgatcgcctc cggttcttgg tttgcttttt gatagacgga
     2041 ttccactgtc gtctcgctga tgaatggcat atccccgaga aacaaaaaaa cgcctgacac
     2101 cttacgacgt atagcctccc tgatcccaac ttttacggac tcggaaagtc cttcccggta
     2161 gcgttcgttg acgaagactt cgacttccct agacgcatcg atcgttcggg cgttaatggg
     2221 gtttgaaacg actagaactc gccacgaatc tagcgagtcc gcacgccgtt gcctgagtag
     2281 ggctttgtcg atcacctgct cgagcaccgt ccgaccggtg tcgggcaata ccgcggcgag
     2341 cttgtttccg ccaaaacgtt ttccttcgcc cgccgcgaga acgatcgaga gaaacgaact
     2401 attctttaaa tcgatagttg atgccctcca gctggatttt aaagttggtt cccaagccca
     2461 ggtagtgttt taactccggt aagcgacgga tgagatcgaa gtattcgtcg ctgaaggcct
     2521 tgacctcgac gacaccctct tctcggaagt cggtgtccac ccaggcgttc tccgcttcat
     2581 cgaacaagaa cacctttcca tacagattcc ggtaaccttt ggcgcttgct cccgtgccgt
     2641 aggctttttc caactcttcg gtcgtcatcg actgcaccat caggttttgc atcttcgagg
     2701 cctcgaaggc gccttgtccc gtctgactca gagtgggtgc tggagcggcg gaagaaaagg
     2761 cgcgcatatc aaaaaggatc ttcggaaccg tgccgtcgac ggactctatg tctttgtctt
     2821 ggcccgttac gccttcgagg atcagatagg aggtgtaggg cgtcggtatg ccgtattttt
     2881 tgctgagccg gacgatctct tcccgcgctt ccactgtctc gccattcaat ttgatgtggt
     2941 ttagcagttc actgatccta cgagaagccc aaagtttcga gatgtaaggg ttcgagtgtt
     3001 cggagagcgt aaccggatag atttgttcga acgcgatcac accgctcccg tctaaggcgg
     3061 cgatcttccc ggttattctt atctgtaatt ctccgtggcc ggtgtagcgg ccgaagagtt
     3121 tcaaagggaa gtccttgtat atcgtaagat ttcgagaggg gaggaggtcc gtgatttgcg
     3181 cattctctcc tgcgatttct acctgaatgt ccgtcatcag gggattcgag acgtttgcga
     3241 agagctggct cactttcgcc tcgatgtttt ctttttcttc gacgtaaaag gccatcccac
     3301 ctgactcttg cgctaacaga tccaacaggc ccgtgttcac gtcataccca acaccgaaaa
     3361 cgaacagttt cgcgccggtc gctttcgccc agcctatcgc gtcggaggtg attttggata
     3421 tgttcaggat accggacgtc ggttggccgt cagtgagaaa gatgacgtaa ttgttctcat
     3481 cctcgtgatt ggtaaaggcg gatagggtcc ccagtctcaa cgcctcgtga atatccgtag
     3541 tgcctcctgc tgacgagccc ttcaaccact tcaacgcatc cggagtatcc ttttgatcgt
     3601 acagggttgt cagaagctct ttgcggaacg gtgtcatctt gtcattaaac aataacaggc
     3661 cgaaccggtc ttcttggttg agtctctcca ggatgaattg agcggctgct aatgcttgcg
     3721 cgtatttcgt accgtacata ctcccggact tgtccaagac gaccattacg ctcttctgca
     3781 gggttcccac atcagggtaa taggggagga tgtccagcat aaacgtgcct atttcgggct
     3841 cttcttcatc attgacgaaa taggcttcgt aacgacgctc tccgtagccg atgaagagga
     3901 cgaaatcttg agaggggaac aggttttctt ccgcataaac aaaggagacc gcttcttgtg
     3961 gttgaggggt gaaggccaag gtgtgcgtcg gcgagttgac ggtatagacc aaacgttgcg
     4021 agtggatgac cccttcgatt tttattctcc cgatgttgcc cttgagcaga gaatcgattt
     4081 tgagcgggta gactaacttg tggtaatcac ctgttttatc gacctcttga tagtattcca
     4141 cctctatttc tcgcttttcg aagggagcaa aggggaaaag gcggatgcgg atcatacgct
     4201 ggccgatata ttccagcaaa gccgggtctc tttgctgcct tacgtagctt tcgtagagca
     4261 agcgcgcctc gtctttctcc ataatggtgc cctcgtagac cacgccgttc acggccatct
     4321 tgaaatcggt gataacggtt tgatcgggta agggaaagag gtagacgacc tccacctttg
     4381 cgttcgactc gttatagaat acttctcgaa tcgaaaactt cgcgatatct tcgttgatat
     4441 cgatatggac gaggtgttct aacatagaaa ccgggtttcg tgggaaaacg acaacgggtt
     4501 gcagatctat ccgttcggaa tacggtatca tcacgccgtt cgcagaaagc agagcggaaa
     4561 gggttatcag ggccagtaac caaaacagat acttacgcac actatcgcct ccatcggaat
     4621 ataattagac acaccgttac tgacggcaaa accccatagc ctattgtacg tggtgccgtt
     4681 cactctttcg gaccgaagaa cgcgttcccc caagcataat cggcggggca taaacggtag
     4741 tgggcgtcaa agacgttctg ctcccccgaa accgttccca taaaggccac acgggtatcg
     4801 acggcgcatt cggaaagggc caggcggata tcccattgca tattgctctc gaacacgttg
     4861 tatttcattt ggcagtcaac ttttcccgac aagacgagac cgtctacgct gttatttttg
     4921 aagctgttgg agaggagctc tatgacggtg tctcctccca aaaagcaggc cttaaggttt
     4981 tcagagaaca ggttcttctc gactcggcac ggtttcttgc cggttaaaaa gagcccggtc
     5041 gcgaaccccc gtacttcgtt gtattggatc accgtttctc cgatgcccaa agagaggacg
     5101 ccgtgactgg acagcgaggc gttttttgac ttcagaaaag gtccgtacaa gatgttgttg
     5161 ccgaagaaca ggctgtggtt gaaggtgacg gcccgtatgc cgtcttggcc agcctgtatc
     5221 gtgttctcga gaatacgggc gttcgacata gccaaagaga ccgcgatcgc gcccccgtgt
     5281 atggtcattc cctcgatggt gagcgcaggg cagttctcga cccagatcgt cggaatcagg
     5341 gtgtcggccg gtctcaacac cacacgtccc cgcccctctc ccttgaggtt cagggccttt
     5401 tggaatcgga tattctcgac ataggtcccg gcgcgaatga gaatggtgtc tccggaagag
     5461 gccgcgaaga cggcttgttt gatcgtgcga tagtcggacg cgcctcctcc caccgttaag
     5521 atcgcagaaa aggatacggt gaaagagaaa atcgacagcg tcaatagaag gatgcctgca
     5581 gttcgtaaac ccataccact accccctatg ttatactttt tggcgcagac gacggtgtga
     5641 tcaccttttg tgttgggaag gcaaatacga ttcccttatc tcggaaagct tcgataattc
     5701 caaaattgat cgcttgttgc gcgtccatat aggccatata gtctcttttg tccacccagt
     5761 agacgatgtc gtatataaaa ctatgggtac cgatgctcga gaggtgggct cttgaaaact
     5821 cgagatcagg attccgatcg acgatctctt ggacgatgcc ggggatggct ttcagggttt
     5881 cgagtggtgt atcgaatgga aggctcaatt ggaacagggc tctcctttgc accatccttt
     5941 tatagttgcg gatagaggat tccgtgagca aagaattggc gatcacgagc tgttctccgg
     6001 tcagactcct gagtcgggtg gtccgcaatc cgatcttttc gatcgtcccg gaatattctc
     6061 cgaagacgat gaagtccccc ggctcgaagg gtttatccaa caaaatcgag aaatagccga
     6121 acagatcccc caggatcttt tgcgacgcca gtgcgaccgc tattcccgta attcccaatc
     6181 ctgtgaggat gccgttgatg tcgtatccga ggctcttcat caccccgacg atgccgagag
     6241 tccaagccac gatcttcaca atcgaggtta ggctttttaa gccctgtatc tgggctgtgt
     6301 tctcggggtg ttttcgcgcg taattttgaa cgccgaaaac gatagtcgca gtcacgaatc
     6361 ggatgatagc gacggtcaac agaatcgagc cgatcgcgtt cacgatgtca cgccaggatt
     6421 ggggcatatc gatgggggtg agcgcgatta tgaataccag ataatagaaa accgggagtg
     6481 tggttgtcac gaggatcgtg ctcagaaagg taaagaacgg ggcgtccgcc ttttttccga
     6541 tcgagcgtag ccgtttagcc gtccaccgtt taccgacgag tagaatgatc acgccaacga
     6601 gggcgacc
//
LOCUS       DAOPXN010000123         6586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11609_length_6586_cov_6.979482, whole genome shotgun sequence.
ACCESSION   DAOPXN010000123 DAOPXN010000000
VERSION     DAOPXN010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11609_length_6586_cov_6.979482"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..883)
                     /locus_tag="PKM49_07280"
     CDS             complement(305..883)
                     /locus_tag="PKM49_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY82651.1"
                     /translation="MENKRKRVGGKTLEIIRLNPELYKGIHLTVRYTSKFYYEIEISK
                     EETGLHFSLVRKPFEQEKEKVFDDTLIEEWLETPQLFGARDNGEIVGYLELSHEQWNN
                     RMRVSNIMVEEPYRGRGIGKALMEKAYQTAVEEKARMLILETQSCNDPAIAFYRACGF
                     EIIGFDLYAYTNNDIENHEFRIEMGKPVLLGE"
     gene            complement(1058..1927)
                     /locus_tag="PKM49_07285"
     CDS             complement(1058..1927)
                     /locus_tag="PKM49_07285"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HNY82652.1"
                     /translation="MNALTIGIFDGIHAGHRRLIRHTIKKRFSKGQAVMLTFRYPPEY
                     YFNPHDFEGLLYRPEEKERRARALGIDRVEALDFPTLKDMTPSEFVDRILGPLDPSWV
                     IVGYNFRFGRHRTGTTDFLRAYGKKKGFQTMVVPPVFVNGIKVSSTIIRYFLKNGEIL
                     KANAFLNQPYLLEGTLARSSNGTVLPSLFSLRFTRTGSGYVPLHPGYYLARNRHLGHA
                     LFLASSRTQRNEPPDFSVHLLGFDRNNPRFVETVDSKTPYDWILLDYLSPRLDRAVLE
                     AARGRGHEAVGSR"
     gene            complement(1931..2902)
                     /gene="truB"
                     /locus_tag="PKM49_07290"
     CDS             complement(1931..2902)
                     /gene="truB"
                     /locus_tag="PKM49_07290"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNY82653.1"
                     /translation="MKDRFSSHHGVLIVNKPPGPSSHDMVYRLRSLLGIQKIGHAGTL
                     DPFASGVLVMGVGQGTKILEYLQHQKKTYRMVLRLGLITDTYDCNGQVVEEREVRFCP
                     EAIREAASAFVGGYAQVPPVYSAKKVQGQRLYDLARKGKIIRMPPKNVEIDRIDVLSI
                     QGADVTLEVVAGEGTYMRSLAMDIGYRLGTGATALSLVRLKSGAFTIEGALELWEQSS
                     KELLIQSLLPIETALSDFKRIYLSEADVLKVRNGNQVYAFFPTTGPAGMYSDLSFEKD
                     ELLLACEQTGKPVAVLRAEVNGSFAASLANRSLNRRIATLVKVFGVM"
     gene            complement(2899..3279)
                     /gene="rbfA"
                     /locus_tag="PKM49_07295"
     CDS             complement(2899..3279)
                     /gene="rbfA"
                     /locus_tag="PKM49_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNY82654.1"
                     /translation="MPKVFRLEKLESEFLRILSLALQDWKNPDFPKEKISFTRVLLSK
                     DKRYLDLYVSIYETTGSLEDREKMFLALQSHTKYFRSVLAKNTHIYSTPEIRMVFDRG
                     IEQSVKMQKIIDDLNIPPSSDRTE"
     gene            complement(3317..4066)
                     /locus_tag="PKM49_07300"
     CDS             complement(3317..4066)
                     /locus_tag="PKM49_07300"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY82655.1"
                     /translation="MFPLKDTIGSLRRPIMTYAIIAVNILVFIFQISLSGQKVVVGTQ
                     VWNGMDAFTFQFGMISARFTDSAYAQTFRAVFPYSASALVTVFTAMFLHSGWLHLISN
                     LWALWIFGDNVEDRMGHWRFLLFYILSDLAGNLMFFVSSLFTKVPLIGASGAIAGVMG
                     AYLVLFPFAKVRTLFILFIFPYIVDIPAPIYLGVWFLLQFFNGATSMLGRAGTSNVAY
                     WAHIGGFVFGLIAYQWFLGRERFVRIGRSAR"
     gene            complement(4975..>6586)
                     /locus_tag="PKM49_07305"
     CDS             complement(4975..>6586)
                     /locus_tag="PKM49_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82656.1"
                     /translation="ILNGRSVSQITNPVISNSTQNKIFVAASVILSNEGKIVGLLGAS
                     VLLKTISEISKAVTLGKTGYGWIVDNTGMFVAHQDQDLVLKTTLSDLSKNGYKGAVEA
                     WDKIKKEGEGTAEMIDPKGQHITLVYVPIPNSPNWFLGASVPTAELMTEANHLILLII
                     VLLVFVIVMIAALSFFMGNAIAKPIRLLSEKALAFGNGDLTVDFHAKGHDEVAHMAGT
                     LQEMAKNLNESMRTINDVTNSVSSSSRELLYVSKEAAEGGLNLSRQAEKIDQNVQNTS
                     ASIEEVTSGIEEIAASANSITKISQDLMEQTDEVAEFAEQGDKSVNEITETIEKAAHQ
                     TEESAELVKKLSDQAKNVGEIVDMIGSISEQTNLLALNAAIEAARAGEAGRGFAVVAD
                     EIRKLAEESRTATGNISRILKEIEQRAQQVNSATVKTVEIVNQTSQQAKTVREQFTHI
                     TEKVEKIHSMTQNMAGNSEEQSAAVDEMATAMDASSRAMADIVQQVEQMTLEVRRQTL
                     LSEKVNQSGKGLEQLSGTLSDQVSKFRT"
BASE COUNT         1529 a         1777 c         1610 g         1670 t
ORIGIN      
        1 ttcctgctga acatttccgc cttatctgga cgtactaaag ttcggcgttc ccaggaaagt
       61 ggggatcgcg gggtcccgtt ggttaccccg cgataacctg gtaggcatcg cggcgtaagc
      121 gccccttctt tctgcggcgg cgcgataccc cgacaacatc gcagtgtaaa aaaagacata
      181 gaggggatcg cggcgtcgca caaaccactt accccgcgat aacccgcaca cggtcatcgc
      241 ggcgcaagca tttaagactt cccagggcgt tccgacggcg ctctcggaac aaccgaatga
      301 atctttattc ccctaggagg actggctttc ccatctcgat tctgaattcg tggttctcga
      361 tatcgttgtt cgtatacgcg tacaggtcaa atccgatgat ttcaaaccca caagcgcgat
      421 aaaacgcgat ggccggatcg ttacacgatt gggtttccaa aatgagcatc cgggctttct
      481 cttctaccgc cgtttgataa gctttctcca tcaaggcctt tccgatacct cgtccgcggt
      541 acggctcttc gaccatgata ttcgacacgc gcattcggtt gttccattgt tcgtggctta
      601 actccaagta ccccacgatc tctccgttat cccgcgcccc gaagagttga ggcgtctcta
      661 accattcttc gatcagtgtg tcgtcaaaga ccttttcctt ctcttgctcg aagggttttc
      721 tcactaaaga aaaatgtagg ccggtttctt ctttagagat ctcaatttcg tagtaaaatt
      781 tggatgtgta ccgcacggtt aggtgtatcc ctttgtaaag ctccgggttt agccgtatga
      841 tctccaacgt tttccctccg actcgctttc gcttgttttc caaaacgcgc tcccgcaggt
      901 cctttcgtgg acaaacaatc cgaaggcgtt agggttttta tggttcattt ttggcattct
      961 agattaccat attttgatgg tttttcgcaa agaatctatc gttaaccgct ctaggagcat
     1021 ttcagatata tgggatcccg gaccccttcg gacaccgtca acgagagccc accgcctcgt
     1081 gtcctctgcc gcgggcggcc tctagaaccg cccgatcgag cctcggcgat aaatagtcca
     1141 agagtatcca atcgtaaggc gttttcgaat ctacggtttc gacgaaccga ggattgtttc
     1201 ggtcgaatcc caacaagtga accgaaaaat cgggaggttc gtttcgctgg gtacgagaag
     1261 acgccaggaa aagggcgtgt ccgaggtgtc tgttgcgggc gaggtagtag ccgggatgaa
     1321 gcggtacata tcccgaaccc gtacgggtga atcgcaacga aaacaaagag ggtaaaaccg
     1381 tgccattcga cgatcgtgcc agcgtgccct ccaatagata cggttggttc agaaaggcgt
     1441 tagcttttaa aatttccccg tttttcaaga aatagcggat gatcgtgctg ctcactttga
     1501 tcccgttcac aaaaaccggt ggcacgacca ttgtctggaa gccctttttc ttgccgtacg
     1561 cccgtaaaaa atcggtggtc ccggtccgat gacgaccaaa tcgaaagtta tatcccacga
     1621 tgacccagga ggggtccagc gggcctaaga tacgatccac aaattccgaa ggcgtcatat
     1681 ctttgagagt cggaaagtcg agcgcttcaa cccggtcgat gcccaacgcc cgagcgcgtc
     1741 tttccttttc ctccgggcga tagagcaacc cctcgaagtc gtgcgggttg aaataatact
     1801 ccggcggata tcggaaggtg agcatcaccg cctgaccctt ggaaaaccgc tttttgatag
     1861 tatggcggat caagcgccgg tgtcccgcgt gtatgccatc gaagatgccg attgttagcg
     1921 cgttcaacgg tcacatcact ccaaagacct tcaccaacgt ggcgatccgt cggttcaaag
     1981 agcggttggc caagctcgcg gcgaacgagc cgttgacctc cgctcgaagc acggccacgg
     2041 gtttccccgt ttgctcgcag gccaacagga gttcgtcctt ttcaaaactc agatcgctgt
     2101 acattccagc cggcccggtc gtcggaaaga aggcgtaaac ctggttaccg ttccggactt
     2161 tcaaaacgtc agcttccgat aggtagatcc gtttgaaatc agataacgcc gtctcaatgg
     2221 gcaacagcga ctgaatcaac aattccttgg aagactgctc ccagagctcc aacgccccct
     2281 cgatcgtgaa agcgccgctt ttcaaccgga cgagagaaag ggctgtggcg cccgtcccca
     2341 aacgataacc gatgtccatc gccaatgacc gcatataagt cccttcaccg gcaacaacct
     2401 ctagagtcac atcggcccct tgtatggata acacatcgat tcggtcgatt tcgacgtttt
     2461 tcggcggcat ccgtatgatc ttcccttttc gcgcgaggtc gtagagcctt tgcccttgca
     2521 ccttctttgc gctatacacc ggcggcacct gggcatatcc gcccacgaaa gcgctcgccg
     2581 cctctcgaat ggcctctgga caaaaccgga cctctctctc ttcgaccact tgtccgttgc
     2641 aatcgtacgt atcggtgatc aatcctaatc gcaagaccat acggtaggtt tttttttgat
     2701 gttgcaagta ttcgagtatt ttcgtccctt gccctacgcc catcacgaga accccggacg
     2761 cgaagggatc taacgttccc gcgtgcccga ttttttgtat ccccaacaag gacctgagcc
     2821 gataaaccat atcgtgtgag gacggacccg gcggcttgtt gacgatcaaa actccgtgat
     2881 gcgacgaaaa gcgatctttc attctgttcg atcggatgag gggggaatat tcaagtcgtc
     2941 gatgatcttt tgcattttta cgctttgttc tatccctcga tcgaaaacca tccgtatctc
     3001 cggagtcgaa tagatgtggg tgtttttcgc taaaacagag cggaaatact tggtgtggct
     3061 ttgaagcgct agaaacatct tctcgcggtc ttccaaagag cctgtggtct cgtagatgct
     3121 gacgtaaagg tccagatacc gtttatcttt ggacagaaga accctggtaa aggagatctt
     3181 ctctttcggg aaatccgggt tcttccagtc ctggagggca agcgacaaaa ttcttaaaaa
     3241 ttcggattcc aatttttcta atcgaaatac ttttggcata ttcttcgctc ctttatttcg
     3301 agcggcgagg tctacctcat cgcgcgcttc tccctattct cacaaaacgc tctcgaccta
     3361 aaaaccattg atacgcgatc aacccgaaca cgaacccacc gatatgtgcc caataggcga
     3421 cgttactggt ccccgcacgc cccagcatcg aggtcgcccc gttgaagaac tgtagaagaa
     3481 accaaacacc caggtagatc ggcgcgggga tatccacgat ataggggaag atgaagagaa
     3541 tgaatagggt acggaccttc gcgaacggga agagcacaag gtacgcgccc atcacgcccg
     3601 caatcgcgcc ggaagcgcct atcagcggca ccttcgtgaa cagggacgaa acgaagaaca
     3661 tcagatttcc cgctaagtcc gacagaatat agaacaatag gaaccgccaa tgtcccatcc
     3721 ggtcttcgac gttatccccg aatatccaaa gcgcccagag attcgaaatc aggtgtagcc
     3781 aaccgctgtg cagaaacatc gcggtaaaaa cagtgacgag cgcgctggcg ctgtagggaa
     3841 agacagcgcg aaaggtctga gcgtaagcgc tgtccgtgaa ccgggcggag atcataccga
     3901 actggaacgt gaaggcgtcc attccgttcc acacctgcgt ccccaccacc actttttgtc
     3961 cggacaagct gatctggaag atgaaaacga ggatgttgac cgcaatgatc gcgtaggtca
     4021 ttatcggtct tctcaaactc cctatcgtgt ctttcaacgg aaacatctct cacctccacc
     4081 tcctctatta taccattgtt ggattgcgta tggcctatcg cgcgtgcgtc aagttatggg
     4141 gtgtactcaa gaaacaagga cagaatcgat cgcgcgattt tgagtccagg tagtggtcac
     4201 ggcgcaagcg ccccttctct ctgtggggcc gacttccagt cggcctgctt ctctgggaac
     4261 gcctgggaac gccgaacttt agtttggcag ggttttatca ttttaactgg gagcgctccc
     4321 gagcgcagca tcgcggcgta agcgcttctt cttttcgcga cggcgcgaaa aatggggttc
     4381 gcacagaggg cacagagagc acggaggaga gattagaacc aatccagaat agagcaggga
     4441 acgtgaatct gggaacgccg gacttaaagt ttgcagattt ttcgtggggt cgtaaaaacc
     4501 acttatcctg cgttaaccca cacgggcatc gcggcgtaaa aaggacaaaa gacggagttc
     4561 ctcctctttc catttttccc gccttgatat tatacccgta tcgacaacgg tcagcaaccc
     4621 agaaggcgat ctctacccat caacggtgta cggtggtctt tcgggacgaa ggggataagg
     4681 tgtaccattt gagggtatct ggaaaaccgg aatctcttca cgaggatatc tacaagaagc
     4741 tcggtgttcc gatgcttaag aacaggcaac tgacttacgt ggctcgtcga ttgtagtggc
     4801 caactttttt gagaaagtgg cttatatcgc cgtttgtagc tcttttagtc gaaagtgggg
     4861 ctaaggagat aacccttcct tagccccttc gaaattcctc caagaaacct gaaaaaacgc
     4921 actaacaaaa aagcgcggcg ggttccgcgc tttttatgta ttagctattc cttctcaggt
     4981 tctgaacttg gaaacctgat cggagagcgt tccggaaagt tgctccaacc cttttccgga
     5041 ttggttaacc ttctccgaaa ggagcgtttg tcttcttact tcgagagtca tctgctcgac
     5101 ctgttgcaca atatcggcca tggcgcggga agaagcgtcc atggcggtgg ccatttcatc
     5161 aaccgcggcg ctttgttctt cggagtttcc tgccatattc tgcgtcatcg aatggatctt
     5221 ttccaccttt tcggtaatat gggtaaactg ttccctgacg gttttcgctt gttgactcgt
     5281 ttgattaacg atctccaccg tcttgacggt cgcgctgttg acttgttggg cgcgttgttc
     5341 gatctctttc agaatgcgcg agatattgcc cgtggcggtt cggctttctt cggctaattt
     5401 tcggatttcg tcggcaacaa ccgcaaaccc tctgccggct tctcctgctc ttgccgcttc
     5461 tatcgcggca ttcaacgcaa gtagattcgt ttgttccgaa attgaaccaa tcatatcgac
     5521 aatttccccg acgtttttcg cctgatccga cagtttctta accagttcgg cggactcttc
     5581 ggtttggtgc gccgctttct caatcgtttc ggtaatctca ttcaccgact tgtctccttg
     5641 ctcggcgaac tcggccactt catcggtctg ttccattaag tcttgagata tcttcgtaat
     5701 gctgttggcg gaagccgcga tctcttcgat accagaagtt acttcttcga tggaggcgga
     5761 agtgttttgc acattctgat cgattttttc cgcctggcgc gaaaggttca gccctccttc
     5821 cgccgcttct ttggaaacgt acaacaactc ccgtgaagaa ctggataccg agttcgtgac
     5881 atcattgatc gttctcatcg attcgttcaa gttctttgcc atttcttgca gcgtacccgc
     5941 catgtgcgcg acttcgtcgt gccctttggc gtgaaaatca acggtcaaat ccccgtttcc
     6001 aaacgccaag gctttttcgg agagcaatcg gatcggtttg gcgatcgcat tgcccatgaa
     6061 gaaagacagg gcggcgatca tgacaatgac gaaaactaaa agaacaatga ttaaaaggat
     6121 tagatgattc gcctcggtca tcaactccgc cgtcggaaca gacgcaccca agaaccagtt
     6181 tggagaattg ggaatcggca catagaccaa tgtgatatgt tggcccttcg gatcgatcat
     6241 ttcggcagtc ccttcgccct ctttcttgat tttatcccag gcttcgacgg ctcctttgta
     6301 tccgtttttc gaaaggtcgg atagcgtcgt tttcagaacc aaatcctgat cctgatgcgc
     6361 gacaaacatg ccggtgttat ccacgatcca tccataaccg gtcttcccca gcgttaccgc
     6421 cttcgaaatt tccgagatgg tttttaaaag caccgaagcc cctaacaatc cgacgatctt
     6481 tccttcattt gacaggatga cgcttgcggc aacgaatatc ttgttttggg tcgaatttga
     6541 aatgacaggg ttcgtgattt ggctgactga gcggccattg aggata
//
LOCUS       DAOPXN010000124         6586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11610_length_6586_cov_5.249120, whole genome shotgun sequence.
ACCESSION   DAOPXN010000124 DAOPXN010000000
VERSION     DAOPXN010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6586
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11610_length_6586_cov_5.249120"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..246
                     /locus_tag="PKM49_07310"
                     /pseudo
     CDS             1..246
                     /locus_tag="PKM49_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892396.1"
                     /note="catalyzes the formation of L-proline from
                     L-ornithine; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
     gene            complement(619..1590)
                     /locus_tag="PKM49_07315"
     CDS             complement(619..1590)
                     /locus_tag="PKM49_07315"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HNY82657.1"
                     /translation="MKLKAKRKAFAQLPTPITTLTQYVEGLTVEVKRDDLTHFLASGN
                     KIRKLEYLFQEAIDKGCRQIVTCGGIQSNHCRATAVMCRENGIKPILFLRGQNPPFPE
                     GNLLIDHLLDAEIHWITSEQYAQRDRLMEEFRLKQDHPEGLYIIPEGGSNALGAMGYA
                     QALLELKAQTDLESLEGIFVPIGSGGTYAGILSALEGLGSKVPVYGINVTKTPSEEFA
                     KRIIDILKEMERRFHMPLAEGYAERVRVLDDYVGEGYAIPTAEGLEQLRSLVSKTGIL
                     LDPVYTSKCFAGMLDICRKNALRRVLFWHTGGGFSIYAYSERILKGV"
     gene            complement(1694..2845)
                     /locus_tag="PKM49_07320"
     CDS             complement(1694..2845)
                     /locus_tag="PKM49_07320"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNY82658.1"
                     /translation="MNQTLGRIPFSETLKYTQLAQKMIEQGKDIVRLTAGEPDFNTPE
                     PVIEAAYTAMKKGMTKYTNSSGIEDLRVGISKMLQRYGIHYKPEEIVVSNGGKHALFN
                     TIYTLTNPGDGVAVITPDWVSYLSQIAICGCRGIQIPTLFKEEFYPRPEVISKHITPE
                     TKVLIVNSPNNPTGAVYSKELLEEIARIAIDKNLWVISDEVYANLIYDGEHTSISTFP
                     GMRERTVVINAFSKSHAMTGWRCGYSAAPLAVSKEIGKLQSHMTSNINAPTQKAALKA
                     LDVDTHEMFDEFKKRRDFICDQLKKIGFSFVYPKGAFYVFMDFNPYMAAFKDDDELAQ
                     KIITDFGVAMIPGSAFHAPGFLRMSYASSIEDLRKAAERLERFLRQYRR"
     gene            complement(2956..3678)
                     /locus_tag="PKM49_07325"
     CDS             complement(2956..3678)
                     /locus_tag="PKM49_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82659.1"
                     /translation="MEERNRCRFPEESREIVERFRSGQAELAFLELKHHIKECPVCAQ
                     RFEAFCVVNRLFTGAKREALFPDYLQRVALRCERAGIALSECMTLYEVFIAHTYTQNM
                     NCDSEIGKELSLAQEFYDLLEVNLPVGSRSTGKPNGFLFRWATLMAPVTIALVFALQW
                     VSGPLLTSNAGLSMAKGVAFPTFDTQAYYTPKEAYTPSKVEPEYRNYTLPVDNALRKT
                     YDLSFDSEETSHSFKIINISFK"
     gene            complement(3755..4633)
                     /locus_tag="PKM49_07330"
     CDS             complement(3755..4633)
                     /locus_tag="PKM49_07330"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNY82660.1"
                     /translation="MYLQDMIRNLDAFWASKGCSILQPYDMEMGAGTYHTATILGALG
                     PKPWRCAFVQPSRRPTDGRYGENPNRMQRFLQYQVILKPSPEESQNLYLESLRALSIR
                     PEEHDIRFVEDNWESPTLGAWGIGWEVWLDGMEITQFTYFQQIAGIDVKPIPLEITYG
                     IERIVMYLQGIDNVYDLKWNEEMTYRELYHQNEVEFSRYNFEKADTDMLFRLFDMHAK
                     ECERLIADKLVRPAYEQMVKCAHTFNLLDARNAISVTQRQAYIGAIRNMTKACAKVYL
                     EALEPEIKNETAPVRG"
     gene            complement(4738..5859)
                     /locus_tag="PKM49_07335"
     CDS             complement(4738..5859)
                     /locus_tag="PKM49_07335"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HNY82661.1"
                     /translation="MGDVFFTDVYESATRYFRTSFRKALGNWKQVSFHQHPVVDGLTI
                     DSYRCEPFLQTKKLLVITGGEHGIEGYAGIAFLDVFQREFLPLIDRDRIGLRVLLPIN
                     PWGMKHHRRVNENNVDLNRTFHPDSSPVDRKVNRAYTDALSLVQEEKPVRGYNLTRFR
                     FACRYLWMARKLGFKDLKKALTFGQFQYPQGLYYGGTQIQPSTAYMKRYFDEVFSSCE
                     DIVHLDIHTGAGPKNRMTIVNSAFSPGSSDDYERLYGYRPVVKATNDEFYAMIGDMID
                     YIYQMAPVKFPNTKFYSTCFEYGLIGESPWGKLKSRQAIVQENQAFHHGAESKSVHRL
                     IRKRFDEVFYSHSEDWHTAMVEDTRLALSGILRAHGFIA"
     gene            complement(5922..6125)
                     /locus_tag="PKM49_07340"
     CDS             complement(5922..6125)
                     /locus_tag="PKM49_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNY82662.1"
                     /translation="MVKGTVKWFNSQKGYGFITQDGGNDVFVHFSAIQTDGFKKLEEG
                     QRVEFEVQKGDKGPQAANVKVIQ"
     gene            complement(6267..>6586)
                     /locus_tag="PKM49_07345"
     CDS             complement(6267..>6586)
                     /locus_tag="PKM49_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82663.1"
                     /translation="VEEGAYQLGIECDGPEYHAVRSTRDRERLHFEVLQRMGWRVLRL
                     WSPSWVQNKGAEQAFLRERLRFEMPSQKPREQGHSNTRIAALPPRSSLSPAERVRSTD
                     DNG"
BASE COUNT         1499 a         1773 c         1643 g         1671 t
ORIGIN      
        1 tgcccgggca agaccgagct gcatcgcgac atcgtgctgc ggtccgagat ttttgtggaa
       61 tatccgccgc agacctggat cgagggtgag atccagcaat tgccggccga tcatcccgtc
      121 accgaactct ggcaggtgat tgccggtgaa tgtcgcggcc gcaccgatcc gcggcagatc
      181 acgttgtttg attccgtcgg ctttgccatc gaagattttt ctgccttgcg ccgctggcgt
      241 gcaagacctc ttggccgctg agatcgcgcg cattacgggg cagtccatag acgacttccg
      301 catcacgcgc ggcggcattg cgctgctggg cagattggct gaccccgatg tattccgcaa
      361 tgctccacca gatgataccg ccaacgaaca caacggcgac cacccagatg atcacttttt
      421 cccacttgct aaaccattcc ctcatctaat tcctcctcat gcgcatcgaa cataatatcg
      481 ccgaattata tcacaattga tcgacggctt ttttgactcc aagcacagaa aactttggga
      541 cgaacaaaga aaaatgcccc aggagaagcc gcttaaaggt atccgcgttt taacgcctaa
      601 atccctttga caagtacgtt aaacgccttt gaggatgcgc tcggagtagg cgtagattga
      661 gaaccccccg cccgtgtgcc agaatagcac ccgcctgagg gcattctttc tgcagatgtc
      721 cagcatcccg gcgaaacact tggaggtgta aacgggatct aggagaatac cggttttgga
      781 gaccagggac ctcagctgct cgaggccctc tgcggtgggg atggcgtacc cctccccgac
      841 gtaatcgtcc aaaacgcgaa cccgttccgc gtagccctct gccagcggca tatgaaaacg
      901 gcgttccatc tccttcaaga tgtcgatgat ccgtttggca aactcctcgc tcggcgtttt
      961 agtgacgttg atgccgtaca ctggcacctt cgagcccagc ccctccaacg ccgacaggat
     1021 gccggcgtag gtgccgccgc taccgatggg cacgaaaata ccttcaagac tctctaaatc
     1081 cgtttgtgcc ttaagttcca agagggcctg tgcgtatccc atcgcgccta aggcgttgga
     1141 cccgccctcc gggatgatgt ataacccttc cgggtggtct tgcttaagcc ggaattcctc
     1201 cattagccga tcccgttgcg cgtactgctc cgatgtgatc caatgtattt ccgcatccag
     1261 cagatggtca atcaacaggt ttccctcggg aaatggcggg ttttgccccc gtaaaaacag
     1321 gatcggtttg atcccgtttt cccgacacat caccgcggtc gcacgacaat ggttcgattg
     1381 tatccctcca caggtaacga tctgccggca tcctttgtcg atagcctctt gaaagagata
     1441 ctccaacttc cggattttat tgccggaagc caaaaaatgg gtcaggtcat cgcgtttgac
     1501 ttccaccgtc aagccttcga catactgggt cagcgtcgta atcggggtcg gtaactgcgc
     1561 gaacgctttc cgtttagctt tcaatttcat cgtccccctc ctattgggaa caaaaaaggc
     1621 gtccgggcgg accacctttt ttgtacgtat tatctatctc cagaggttct tgcctgaata
     1681 caaaatcgaa cgcttagcgg cggtattgtc gcaaaaaacg ctccagacgc tcggccgctt
     1741 tcctcaaatc ctcgatcgat gaggcgtaag acattctgag aaaaccgggc gcgtggaaag
     1801 cgcttccggg gatcatcgcg accccgaaat cggtgatgat tttttgagcg agctcgtcat
     1861 catctttgaa agcggccata tacgggttga aatccataaa cacgtaaaag gcgcccttgg
     1921 ggtaaacgaa ggaaaagccg attttcttca gctggtcgca aatgaaatca cggcgttttt
     1981 taaactcgtc gaacatctcg tgggtatcca catctaacgc tttcaacgcg gctttttgag
     2041 tgggggcgtt gatgttggag gtcatatgcg actgcaactt tccaatctct ttcgagaccg
     2101 ccagaggggc ggcggagtac ccgcatcgcc agccggtcat cgcatgcgat tttgaaaagg
     2161 cgttgatcac gacggtgcgc tctcgcatac ccgggaaggt cgagatgctg gtgtgttctc
     2221 cgtcgtagat caggttggcg taaacctcgt ccgagatcac ccagaggttt ttgtcgatgg
     2281 cgatacgcgc gatctcttcg agtaattctt tcgaatagac ggcgccggtc gggttgttcg
     2341 gcgaatttac gatgaggact ttggtttcgg gagtgatgtg cttcgagatg acctccggtc
     2401 tcgggtagaa ctcttcctta aaaagcgtgg ggatttgtat cccgcgacat ccgcaaatcg
     2461 cgatttgaga caggtagctg acccagtcgg gggtaatcac cgcgaccccg tctcccggat
     2521 tcgttaacgt gtagatcgta ttaaacaggg cgtgctttcc gccgttggaa acgacgattt
     2581 cttcgggctt gtaatgaatg ccgtagcgct gaagcatctt cgaaatcccg acccgaaggt
     2641 cctctatacc ggaactgttc gtatacttcg tcattccctt tttcatcgct gtataagcgg
     2701 cttcgatgac gggctctggc gtattgaagt ccggttcgcc cgcggttaat cgaacgatgt
     2761 cctttccctg ttctatcatc ttttgcgcca actgggtata cttcaaagtt tcagaaaacg
     2821 gaattcttcc gagtgtttga ttcatctctt ggcttctcct ttcccattat aatgatgagc
     2881 ataaaaaaag atcaaaaaac cgtggttttt tgatcttcag acagacgata aacagcataa
     2941 agaaccttta agaatctatt taaaagaaat attgatgatt ttgaacgaat gcgacgtctc
     3001 ctcggaatcg aaggacaagt cataggtttt cctcaaagcg ttgtcgaccg gcaaggtgta
     3061 gtttctgtat tccggctcca cttttgaggg ggtataagcc tccttcggcg tgtaataagc
     3121 ctgggtatcg aaggttggaa aggccacgcc ttttgccatt gaaagtccgg cgttggaagt
     3181 caacaggggg cctgaaaccc attgaagagc aaagaccaac gcgatcgtaa ccggcgccat
     3241 taaggtcgcc catcgaaata aaaacccgtt tggcttaccg gtgcttcgac tgcccacagg
     3301 taaattgacc tccaaaagat catagaactc ttgggccaaa gacagctctt tcccgatttc
     3361 ggaatcgcag ttcatatttt gcgtgtaggt gtgcgcgata aaaacctcgt acagggtcat
     3421 acattccgaa agagcgatgc cggcgcgctc gcacctcagg gccacacgct gcagatagtc
     3481 ggggaacagg gcctctctct tggctcctgt gaacaaacgg ttgacgacac aaaaagcttc
     3541 gaagcgctgg gcacatacgg ggcattcttt gatgtggtgt tttaactcga gaaaagcgag
     3601 ctctgcttga ccggatctaa accgctccac gatctctcga ctttcctcgg ggaatcgaca
     3661 tctattcctt tcttccattt tttcacccat cctgcaaatt ctcttcagct ttttattctg
     3721 acgaaatccc gttgtttttt atttcctgaa actatcatcc tcgcaccggc gctgtctcat
     3781 ttttaatttc gggttccaac gcctcgaggt aaaccttggc gcacgctttc gtcatattcc
     3841 ggatggcgcc gatatacgcc tgccgttggg tgacgctgat cgcattccga gcatccaaca
     3901 agttgaaggt gtgggcacac ttcaccatct gctcgtaagc cggcctaacg agcttatcgg
     3961 cgatcaagcg ctcgcattct ttggcgtgca tatcgaacag ccgaaagagc atatcggtat
     4021 cggctttctc gaaattataa cgcgaaaact cgacctcatt ttgatgatag agttcgcggt
     4081 aggtcatctc ttcattccat ttcaggtcgt agacgttgtc gatgccttgt aggtacatca
     4141 cgatgcgttc tatgccgtag gtaatctcga gcgggatcgg tttcacgtcg attccggcga
     4201 tctgttgaaa atacgtgaac tgcgtgattt ccataccgtc cagccagacc tcccaaccga
     4261 ttccccaagc gccgagcgtc ggagactccc agttatcctc cacaaaacgg atatcgtgtt
     4321 cttccggtcg aatgctcaac gccctcagag attcgaggta aaggttttgg gactcttccg
     4381 gagagggttt gagtataacc tgatactgaa ggaaccgctg catccggttg gggttttctc
     4441 cgtaccttcc gtcggtgggc cttcgactgg gctgaacgaa ggcgcagcgc cagggtttcg
     4501 gccccaaagc gcccaagatc gtcgccgtat ggtaggtgcc cgcgcccatc tccatatcgt
     4561 agggctgcaa gatagaacac cctttggaag cccagaaggc gtccaggttt cgaatcatat
     4621 cttgtagata cataaacctc acccgatttt tgtcgctgta aggaatcaac gagtttattc
     4681 tatcatacgc gccgcgggtt aatgaaagaa aaagtgaaaa atggttcttt cgggtcgcta
     4741 cgcgatgaaa ccgtgagcac gcaagatacc cgagagcgcc aatcgcgtat cctcgaccat
     4801 cgccgtgtgc cagtcttccg aatgggagta aaagacctcg tcgaaacgct ttcgtatcag
     4861 gcggtgaacg gattttgact ccgccccgtg atggaaggct tggttttcct ggacgatcgc
     4921 ttgccgactt ttcagttttc cccaggggct ctcccctatc aagccgtatt cgaagcatgt
     4981 ggaataaaac ttcgtattcg ggaatttgac gggcgccatt tgatagatat agtcgatcat
     5041 atctccgatc atcgcgtaga actcgtcatt tgtggccttc acgacggggc gatacccgta
     5101 caggcgttcg tagtcatcgg acgatcctgg agaaaatgcg gagttcacga tggtcatccg
     5161 gttttttggc cccgccccgg tatggatatc cagatgcacg atatcctcac aggaggagaa
     5221 gacttcgtcg aagtaccgct tcatatacgc ggtggaaggt tggatttggg tccccccata
     5281 gtagaggcct tggggatatt ggaactgccc gaaggtcagg gccttcttca aatctttgaa
     5341 tcccagcttc cgggccatcc aaaggtaccg gcaggcgaaa cgaaaccgcg tgaggttgta
     5401 gccccgcacc ggcttctctt cctgcaccag gctcaatgca tcggtatagg cgcggttgac
     5461 cttgcggtcg accgggctcg aatccggatg gaaagtgcga ttcaaatcca cgttgttctc
     5521 attcacccga cgatgatgtt tcatccccca aggattgatt ggtagtagca cgcgtaggcc
     5581 gatccggtct cggtcgatga gcggcaggaa ctctctctga aagacatcca aaaacgcgat
     5641 ccccgcgtac ccttcgatcc cgtgttcgcc gcccgttata accagtagtt tttttgtctg
     5701 aagaaagggt tcgcaccggt aagagtcgat ggtgaggcca tcgacgacgg ggtgttgatg
     5761 aaaagaaacc tgtttccaat tacccaaggc ttttcgaaag gatgtcctga aatagcgcgt
     5821 cgcactttca tagacatctg taaagaaaac atcgcccata gtccccctga aaaagcaaaa
     5881 cgggggaatt ctccccccgt tttagcgatc gaatagtatc cttattggat cacttttaca
     5941 ttcgcagctt gaggtccttt gtctcccttt tgaacttcga attcgacgcg ttggccttct
     6001 tccaattttt taaagccgtc agtttgaatt gcggagaaat gtacaaaaac gtcgttgcct
     6061 ccatcttgcg tgatgaagcc gtaccccttt tgggaattga accacttcac tgtacctttt
     6121 accatgttgt cttttcctcc agaaattatt tcaccatctt tttgatggca cggtataata
     6181 ctcttttttc accggtttgt caagtgatcg cctcacttca taaaaacccc ttaaagtgaa
     6241 ggacagcacg ccacgaaccg gctcttctac ccgttgtcgt cggttgacct aactctttca
     6301 gccggggaca gcgaacttct tggaggtaac gcggcgatcc gggtgttcga atgaccctgt
     6361 tctcggggtt tttgtgacgg catttcgaat cgaagacgct ctcgtaagaa ggcctgctcc
     6421 gctcctttgt tttggaccca gctcggtgac cagaggcgta acacacgcca tcccatcctt
     6481 tgaagcacct cgaaatggag gcgctcccga tccctagtag atcgaaccgc gtgatactcc
     6541 ggaccgtcgc actcgatccc caactgataa gcgccttcct cgactg
//
LOCUS       DAOPXN010000125         6555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11683_length_6555_cov_5.982923, whole genome shotgun sequence.
ACCESSION   DAOPXN010000125 DAOPXN010000000
VERSION     DAOPXN010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11683_length_6555_cov_5.982923"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(459..3032)
                     /gene="glgP"
                     /locus_tag="PKM49_07350"
     CDS             complement(459..3032)
                     /gene="glgP"
                     /locus_tag="PKM49_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNY82664.1"
                     /translation="MPTFQTLSVLPRLPERLKGLKDLAYSFWSSWNPEVESLFRGLNP
                     RLWSGVQYNPAKFLRYISQRRLDAAAKNPDFLREYDRIVSLYERHRDVASLWFSQTYG
                     KPGKENFQIGYFSAEFGLHESFPMYSGGLGILAGDFIKSCSDMGLPIVAVGLLYKNGY
                     FEQQLNREGWQEALYHSYSFMDLPVETIKNDFGDEIKVSVDFPNGTVLAKVWKASVMN
                     TTLLLLDTDLPENTPENREITSKLYGGDQDMRVRQEMILGIGGVRALRACGYQPSVWH
                     MNEGHSVFMALERIRELVEKESITFHQALEIVRASTVFTTHTPVPAGNDAFHYGLIEK
                     YFYTYWVRMKISKETFLNLGTAPYEEGQRMFNLTILALKLCAWKNGVSRLHGRVSQNL
                     WKNNWVGVPTNEIPISYVTNGIHTETWMNRNIRGLFDKYLPEWKDRFLDRAYWDGIDA
                     IPDRELWEVTRKMKKEMIDFIHGRVSEQRSRYGETIEQLREVDSIFDENTLTIGFARR
                     FATYKRATLILQNLERFKRILNDPERPVQIIFAGKAHPADRPGQELIKRIYEISRSWD
                     FKNRIVFLENYNMEVARNLVSGVDIWLNTPRRPHEASGTSGQKVPVNGGINFSVLDGW
                     WEEGYDGKNGWIIGDGREYADTNIQDTVDAVSFYDTLEKEIVPKYYQRNEEGIPVEFL
                     AMMRRSMKTVIPLFNTHRMVKDYLDELYLPAYSFGSALEEKRFETGKAMAEWKTHIEK
                     EWRAVKIQSNTIRRDEEEIPANLELEFSATVYLGEIEPKDVAIEIYFERYNVMGELEG
                     TETFPMAMDMETLHHTYVFKGKFRLSERGNYKYSIRALPYHPALPLKFDTGLIRWMGE
                     A"
     gene            complement(3067..3993)
                     /locus_tag="PKM49_07355"
     CDS             complement(3067..3993)
                     /locus_tag="PKM49_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNY82665.1"
                     /translation="MVRFFSRENEKVVEKNGFSQDCIILVVNPTREEAAMISSLLDFT
                     PDFIHDVQDLDERARIDYGEKVIQIILKVPYKDASDEKLLYKTIPLNILLGENYILLG
                     SKYPVEFVQKMIDESKVNPKKRSRMIFQLMYKNAILFLNHLKEINKITDSIEEKLQEA
                     MKNKELQQLMYLEKSLVYFTTSLRSNEIVMERILRGNVFEIFDEDRELLEDTIVENKQ
                     ALEMANIYSSILSGMMDAYASVISNNVNSIMKVLTIVTIVLQIPAILSSFYGMNIPLP
                     FQQEGLVFIFILAWSVVIGMLILLWFKKKKWI"
     gene            4706..5917
                     /locus_tag="PKM49_07360"
     CDS             4706..5917
                     /locus_tag="PKM49_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006490404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82666.1"
                     /translation="MRSQITQILLFTSIGTITRDMFGVVFNLYLRENRFSNQEIGSIT
                     SFMLWGSAIVGLVLSLLAETIGRKRVLFVSAFLFPIFNLGMILIPNPGTLNALSFFRG
                     GIGVISQTVVSAIIVHLTESRNRAKVFGLNIGLTTASAVLGNLLGGFFGEWFGLREAL
                     YLSTGLYCLSFIPLLRIKIFEVKVAIRQVLDFSMIQREQKNILTLYYLSTLSVGFGAG
                     LFIHFGNLIYKDLFGLSSAAIGIALSIADLGTSAGNIFSHVLARRVGPLQLRFWCELL
                     VVPLMISLIVVRQPALFVLLYTLRYILMNSASPILSTIINSFVPKEKIATLAGINTFL
                     NNSIRGVAAMLFGIIVGASLSGYNLLFSISTAFYALNAVFSFLFLVRYRRHPIVLSLY
                     PNRKDSLPSSE"
     gene            6408..>6555
                     /locus_tag="PKM49_07365"
     CDS             6408..>6555
                     /locus_tag="PKM49_07365"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82667.1"
                     /translation="MRLSSGGIPVKKISKPHDKLFKEVMGDIETAKSFIQHYLPPKIV
                     RLIDP"
BASE COUNT         1481 a         1727 c         1611 g         1736 t
ORIGIN      
        1 gcgttgtttt ttcttatttt ttcccttttt ctccaaaacc ggtcgaaagt cgggtcaggg
       61 ccatcccaga gacaagccga ctggaagtca gccccacaaa gagaaagggc gcttacgccg
      121 cgatgctgcg ctcgagaacg ctcccagtga aaagatagaa ccatcccaaa ctaaagtttg
      181 gcgttcccag aagtgctttc ccagtgaacc tccctccgtg acctccgtgt tctctgtgtg
      241 aaaccctgtt ttctctattc ataagacctg ccaatccaaa gattggcgtt cccagtaaac
      301 ctgtcgcatt aaaaggcagc attcccagaa gtacgttctc agtgaacctc cctccgtgac
      361 ctccgcgtga ccccttgttt gattctaaga catgccgact acaaaagtcg gcgttcccaa
      421 gatgctatcc ttagcccgca atccgctgtt gaaccaggtt aggcttcgcc catccaacgg
      481 atcagacccg tgtcgaactt caacgggagc gctgggtggt agggaagcgc ccggatgctg
      541 tacttgtagt tcccccgttc cgataatcgg aacttccctt tgaaaacgta cgtgtggtga
      601 agggtttcca tatccatcgc catcggaaag gtctccgtgc cttctaactc tcccatcaca
      661 ttgtaccgct cgaaatagat ttcgattgcc acatccttcg gctcgatctc tcccagataa
      721 acggtggccg agaactcgag ctcaaggttc gcagggatct cttcttcatc cctccggatc
      781 gtattcgatt gtatcttgac ggctctccat tctttttcga tatgggtctt ccattcggcc
      841 atagcctttc cggtttcgaa tcgcttttct tccaaagcgg agccaaaaga ataggcgggc
      901 aggtagagtt catcgaggta gtctttgacc attcgatggg tgttgaacag cggaatgacc
      961 gttttcatcg aacggcgcat catcgccaag aactcgacgg ggattccctc ttcgttgcgc
     1021 tggtaatact tcgggacgat ctctttttcc aacgtgtcgt aaaaagagac cgcgtcgacg
     1081 gtgtcttgga tattcgtatc ggcgtattca cggccgtctc cgataatcca gccgttttta
     1141 ccgtcatagc cctcttccca ccagccatct aggacgctaa agttgatacc gccgtttacg
     1201 ggcaccttct gaccggacgt gccgctcgcc tcgtggggcc gcctcggcgt gttcagccag
     1261 atgtcgactc cggataccag atttctagcg acctccatat tgtagttctc caaaaagaca
     1321 attcggttct tgaagtccca agaacggctg atctcgtaga tgcgcttgat caactcctgg
     1381 ccgggacggt ccgcggggtg cgccttaccg gcgaagatga tctgtaccgg gcgttctggg
     1441 tcattcaata tccgtttgaa ccgttcgagg ttttgcaaga tcaaagtggc gcgcttgtag
     1501 gtggcgaacc tcctggcaaa gcctattgtc aaggtgtttt cgtcgaagat gctgtcgact
     1561 tcccgaagct gctcgatggt ctctccgtac cgggaccgtt gttccgacac ccgtccgtgg
     1621 atgaagtcta tcatctcttt tttcatcttt cgagtgacct cccacagctc tcgatccggt
     1681 atcgcgtcga tgccatccca ataggcgcga tccaaaaatc ggtccttcca ctcggggagg
     1741 tatttgtcaa acaggcctcg gatgtttcgg ttcatccacg tctccgtgtg gatcccattg
     1801 gtgacatagg agatcggtat ttcgttcgtg ggcacgccga cccagttgtt tttccacaag
     1861 ttttgagaca ctcgtccgtg caagcggctg acgccattct tccacgcgca gagtttgagc
     1921 gctaagatgg tgaggttgaa catcctctga ccctcttcat aaggggctgt tccgaggttc
     1981 aaaaaggtct ctttggaaat cttcatacga acccagtagg tgtagaagta tttttcgatc
     2041 agcccgtagt gaaaggcatc gtttccggcc ggaaccggcg tgtgggtggt gaagaccgtc
     2101 gacgctctga cgatctccaa cgcttggtgg aaggtgatgc tctctttttc cacgagttcg
     2161 cgtatccgtt ccaaggccat gaagacggag tgaccctcat tcatatgcca aacggagggt
     2221 tggtacccgc aggcgcgtaa ggcacgaacc cccccgatgc cgaggatcat ctcctgtcga
     2281 acgcgcatat cctggtcacc gccgtagagt ttcgaggtga tttcccgatt ttccggggtg
     2341 ttttcgggca agtccgtatc gagcagcaac aacgtcgtat tcatcaccga ggccttccaa
     2401 accttggcca agaccgttcc gttcgggaag tccacgctga cttttatttc atctccgaaa
     2461 tcgtttttaa tcgtttcgac gggtagatcc atgaaggagt aggaatggta aagcgcctct
     2521 tgccatccct cccggttcaa ttgctgctca aagtagccgt ttttgtaaag cagtccgacg
     2581 gccacaatgg gaagacccat atcgctgcag gatttgatga agtcgccggc caggatacct
     2641 aaccctcccg agtacatagg aaaggattca tgtaatccga attcggcaga aaagtacccg
     2701 atctgaaagt tttctttccc aggtttgccg taggtttggc tgaaccacaa agaggccaca
     2761 tctcgatggc gttcgtaaag tgagacgatg cgatcgtatt ctcttaaaaa atccggattt
     2821 ttcgccgccg catccagcct tctctggctg atgtaccgta ggaatttcgc cgggttgtat
     2881 tgaacgccac tccacaagcg ggggttcaat ccccgaaaca gcgactccac ctccggattc
     2941 caactgctcc aaaagctgta ggccaggtct tttaaccctt tcagtcgctc gggcaacctg
     3001 ggtagtaccg acaaagtttg aaaagtgggc atcattaccg ctcctttcac cgatttctag
     3061 acgatcctat atccattttt tctttttgaa ccacaggagt atgagcatac cgatgacaac
     3121 cgaccacgcc agaatgaaaa tgaagaccaa cccctcctgc tggaacggca ggggaatatt
     3181 cataccgtag aaactggaga ggattgcggg tatttggagc acgattgtga cgatcgtcaa
     3241 gaccttcata atcgagttga cgttgttcga tataacggaa gcatacgcat ccatcatccc
     3301 agacaaaatg ctgctataga tgttcgccat ctccaaagct tgcttgtttt ccacgatggt
     3361 gtcttccaag agctcgcggt cttcatcgaa aatctcgaat acgtttcctc gcaaaatccg
     3421 ttccatcacg atttcgttgg atctcagaga tgtcgtaaag tataccaagc ttttctctag
     3481 atacatcagt tgctggagct ctttgttctt catcgcttcc tgaagctttt cctcgatcga
     3541 atccgtgatt ttgttgatct ctttcaagtg gttgagaaac aggatcgcgt ttttatacat
     3601 cagttggaag atcatccggc tccgcttctt ggggttcacc ttcgattcgt cgatcatctt
     3661 ttggacaaac tcaaccggat acttgctgcc caacagaata tagttttcac ccaaaaggat
     3721 gttcaatgga atcgtcttat agagcagctt ttcgtcggac gcatccttgt atggaacttt
     3781 taggatgatc tgtatcacct tttcaccgta gtcgatccga gcgcgctcat ccaaatcctg
     3841 aacgtcatgg atgaagtcgg gtgtgaaatc gagcaggctg gagatcatag cggcttcctc
     3901 tcgagtggga ttcaccacga ggataatgca gtcttgggaa aagccatttt tctccaccac
     3961 cttttcattt tcgcgtgaga aaaatcgaac catcgggtat tacctcctcg cttcatcgta
     4021 cgcattacgg aggccgcgtc ggcatttctg cgtttctcgc cacttccgtc tacgctcacc
     4081 ttacctcttc acaagaacgg cgccgtgttc ggccttattc taccatacgc gatgatttct
     4141 cgcagtgaag gtgtgggtca agttatgagg agtaatcaag aaacaaggcc agaatcgatc
     4201 gcacgatgtt tagtccttgt agtcatcacg gcgtagaatg ggcaagggga aatcgcgggg
     4261 tcccggcggt tatcgcggcg taagcgcttc ttctttttgc gagggcgcga tacgcttggg
     4321 aacgccaact tttaagttgg catgtcttta aaaaaactca aacggagacc acggaggtca
     4381 caaagaagag aggtgtttag gagagtcaac accaggttta tgtgtcctct gtgctctctg
     4441 cgcgctctgt gagaaaccat cttttttgga catgccgaac taaagttcgg cgttcccagg
     4501 cgcaccatcg cggcgtaagc gcttcttttt tatgcgacgg cgcgatatgc ttttattcga
     4561 gcagatcgcg gcgtaaaaaa ggcggaaggc gttgcccctc gatattatac ccacaccgtg
     4621 agccctcatt gatttatcag cgaaaatagc ctataatacc cgcgaaagtt tgtttaccgt
     4681 actataaaga tgaggggaac ggaagatgcg ctcgcaaatc actcaaatac tgttgttcac
     4741 gagcatcggc acgattacca gggatatgtt tggagtggtg ttcaatctgt atttgcgaga
     4801 gaaccgtttc tcgaatcagg agatcggcag catcacctcc ttcatgcttt ggggcagtgc
     4861 gatcgtcggt ctggtgttga gcctcttggc ggaaacgatc ggaaggaagc gagtgctgtt
     4921 cgtctcggcc tttctgtttc cgatcttcaa cctcggtatg atcttgatac cgaatccggg
     4981 gacgctcaac gcgctctctt tcttcagagg aggtatagga gttatctctc aaacggttgt
     5041 ttcggccatc atcgtccatc tgaccgaaag ccggaatcgc gcgaaggtct tcggactgaa
     5101 catcggtttg acgacggcca gcgcggtgct cggtaacctc ctgggagggt tcttcggaga
     5161 gtggttcgga ctacgcgaag ccttgtatct gtcgaccggt ctctattgtc tgagcttcat
     5221 cccgctacta cggatcaaaa tcttcgaagt caaagtcgcg atacgtcaag tgttggattt
     5281 ttcgatgatc caacgcgagc agaaaaacat cctgacgctc tactatctgt cgacgctgtc
     5341 ggtcggattc ggagcgggac tgttcatcca cttcggcaac ctgatttaca aagacctctt
     5401 cggcctttct tccgccgcca tcggtatcgc gctctcgata gccgatttag gcacatcggc
     5461 gggtaacatc ttttcgcacg tcttagcgcg gcgcgtagga cccttacagc tgcgtttctg
     5521 gtgtgaattg ctggtcgttc ccttgatgat ctctctcatc gtcgtacggc agccggcgct
     5581 gttcgttctg ctctacacgc ttcggtacat cctgatgaac tcagcctcgc caatactttc
     5641 caccatcatc aactcctttg tcccgaaaga gaagatcgcg actttggcgg ggattaacac
     5701 gtttctaaac aattcaatac gcggggtcgc cgcgatgctg ttcggaatca tcgtcggggc
     5761 cagcttgagc ggatataacc ttctgttctc gataagtacg gcattttatg ccttgaacgc
     5821 ggtcttttcc ttcctctttc tcgtccgata cagacgacac ccgatcgttc tgtccctcta
     5881 ccccaatcga aaagactcgt tgccttcttc ggagtgagaa gcgcgatgga gaaagtggcg
     5941 tcgcgctttt gaccccagcg tcaaccctcc gtgtgggtca agataagggg tgcaatcaag
     6001 agacaaggct agaaatcgtg acataagcgc tccttctctt cgtggggccg acttttggtc
     6061 ggcctttctc tgggaacggc agctttttag tttgcatatc ttaaaataaa caggggttct
     6121 cacagagagc acagagagca cagagagcac agagagaaca ctaagatttt cacagtgagc
     6181 gcccaggtaa cgctctgggt acaccctggg aacgccaaac tttagtttgg cacggttttg
     6241 tcttttcact gagggtgttc ccgaaggcag catcacggcg taagcgcttt tgcttttccc
     6301 gatggcgcga tacggttggg aacgccaact tttaagttgg catatcttga aaaaaaggcg
     6361 gagccactat cccttatcgg ataacctacc tttaaggtat aataccgatg agactttctt
     6421 ctggagggat acctgtgaag aagattagta agccacacga caaattgttt aaagaagtta
     6481 tgggagatat cgaaaccgca aagtccttca tccagcacta tctgcctccg aagatcgtcc
     6541 ggttaatcga tcccg
//
LOCUS       DAOPXN010000126         6536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11724_length_6536_cov_5.026231, whole genome shotgun sequence.
ACCESSION   DAOPXN010000126 DAOPXN010000000
VERSION     DAOPXN010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11724_length_6536_cov_5.026231"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1396)
                     /locus_tag="PKM49_07370"
     CDS             complement(260..1396)
                     /locus_tag="PKM49_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011308850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNY82668.1"
                     /translation="MIYADNAATTKIPDTVLEKMLPFLQGEYGNASSQHSFGVKARRA
                     ITQARRQVAEAIGAEETEIFFTSGGTEANNWVIRAIANAYRGKPTHMVTSEIEHHSVL
                     DTCRALERSGVEITYVPVNEKGMVDLTALENVIKPHTRLVSIMLANNEIGTVQPVDAI
                     GKLLRNRGVLFHTDAVQAVGHIPVDLSRLPVDFLTASAHKFNGAKGAGILYKRAEIEL
                     APLLTGGEQERGLRAGTENVAGVVSAGYALEESFGVMDSEAKRLRKMVEDTVDGIKAK
                     IPTVWVNGDSNPRLPGLINLGFEGVSGEALMNVLSLKGVCVSTGAACVSGKHAPSHVL
                     LALGLSEQRAQSAIRISYGRYNTPDEVERVVAAVCEAYYKILHA"
     gene            complement(1461..1670)
                     /locus_tag="PKM49_07375"
     CDS             complement(1461..1670)
                     /locus_tag="PKM49_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82669.1"
                     /translation="MKAIRKANCYICGKKDLTRDEVGLNIKLIGREQRNLLCMRCLAE
                     HLEIAVEDLEERIQEFKDAGCELFG"
     gene            complement(1674..3116)
                     /gene="brxL"
                     /locus_tag="PKM49_07380"
     CDS             complement(1674..3116)
                     /gene="brxL"
                     /locus_tag="PKM49_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNY82670.1"
                     /translation="MIDKLRSCFEEMVVFKDLKKSNFFASLSLPSFMRDWLLKKFMLE
                     TGEFDQEEMLAFIRKNIPRKDDWTAIKSKIVTEMERVRFLAKVSADVDIKTGGVSFSL
                     PDFGLTTKETRIPPEVWFTCSNDLTKSREIWGLVELEYLEPDDGLKRKGKIQLRSFKN
                     FCPYVVDVDYYKDTRNEFTTKEWIDVLLGAIDYNADGYANDDAKLTMLTRLLPFVEKR
                     LNLVELAPKGTGKSYLFGRVSRFGWLSSGGLMTRAKLFYDIQRKEHGLIAGHDFIVFD
                     EVQTINFHPLDEMRAALKGYMENGVYTVGKVEGTSDAGIVLCGNIPRDKMSEYESMFE
                     ELPPVFHESALIDRFHGFIEGWKIPRMNDDMKISGWALNSEYFCSILHELRDDPSYRA
                     IVDALIVVPEAADTRDTEAVKRIATAYLKLLFPNVRAPEDIEPIEFRRYCLRPAMQMR
                     HLIKIQLGLLDSEYKGKSIPAFTVRESSES"
     gene            complement(3129..5303)
                     /gene="pglZ"
                     /locus_tag="PKM49_07385"
     CDS             complement(3129..5303)
                     /gene="pglZ"
                     /locus_tag="PKM49_07385"
                     /inference="COORDINATES: protein motif:HMM:NF033445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-4 system phosphatase PglZ"
                     /protein_id="HNY82671.1"
                     /translation="MRDTIAKIKQYFGSASQFPLLVVVSPEEYQEVLQANQQEKIIYV
                     SGYCVGADKEPDIAKLEKDVRSSQGNSLLLGFGDYLVSKAGEAKKYLTRYKAMTLQPK
                     SHVVILLGASMYPVLKDIIKEDPRTVSRVVLPEDVPQNFVVNEDEVVYGIKAYLKACE
                     MGNPPGKVKTALRIANVTVIDPENHFAELNNRYPAVFKNLSPANGTPEYWRKLLAAFD
                     GNEKDFRRYLEGHRFSPPELCFLKYAKSNDYVSWLYFIWLKIFANSRDYLGFVVSKTT
                     TSDDLLETAKNAILDIGLSDSRFPVFYEQRKTLLKGCSDADIANFIPLIHRHGTDRIA
                     YLTDNTKREKQEVLVALGEGAKGDYLQTSYPALFAYLQDYRIVDESLTAYFSAYKKCK
                     VYNTVDASFAELVKVYGAKRPYNSLPTRSTVFSDIKTDRTLLIFLDALGVEYLGYIQK
                     LCAEFKLKYDPTIARANLPTITGVNREFYDEWQGQKENPIKTIDDLKHNPERGYDFHK
                     SPYPIHLPEELDAVREAIERAAVKLRAGDFDTVIIASDHGASRLAVISPDIIIDSGGC
                     EAKSSGRYCQGAVLPKGDNITKENDYAIVADYSRFRGSRSASVEVHGGATLEEILVPI
                     IMLTLETRSIQVTFENNVAEVSHKKAAELILIITPDCDNVMTRVNGTNYKAEKLEKSR
                     FRVVLQGLKKGQYKMEIYENQNYIVSKDFTIKSKGIEVRDDF"
     gene            complement(5303..>6536)
                     /locus_tag="PKM49_07390"
     CDS             complement(5303..>6536)
                     /locus_tag="PKM49_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82672.1"
                     /translation="SGNIGKFVMHDNALANKLKALIKTENFDSALIDFLAGFEAGKLR
                     QLAKEIRVSDDRVVSDIKRHFGNDTEGLWLWNEETGEGQIRKVILEYEFIKKSNELLA
                     KDTQTVGASFEAWRDKLKFVKVSHEVVKKLPEYASFAPALYNVAKSIGTDGIKAFYDV
                     IVKYGYSIVSFLKNDKEAFTHACAFQLQGFSEAEISDIYGKIPINCFEMNERDFISKV
                     GEIVSDFQSNLARLQLRQLWKEKTGTDDPREWSTRYRTPILACVPTGKWDDFKRSFDA
                     VSFNHSTESEVKFALEFLTGISYWENFRNQETVDRAFQDAILGRYKAVLTDLNEVREH
                     LSKRTRVSPYDWSGHPDVNWLIQELAQKKYSQEPFQRVIRRIDSMDSDKLKEYLKRLV
                     EGNMTVGIEILEDGGVGS"
BASE COUNT         1437 a         1729 c         1536 g         1834 t
ORIGIN      
        1 tgagaaagtg gcttttatcg ccgtttgtag cacttttagt cgaaagttgg gttaaaagtt
       61 ggcattccca ggcgttccca ggggctttcc agagagacag gccgacttcc agtcggcccc
      121 gcgatttttc ttcgttctct tctctgtgtt ctctgcgccc tccgagcgag cttttttcaa
      181 gatatgccaa cctcaaagtt ggcattccca aggcgctctc atgcgctccc agggctcggc
      241 ggtctcggta gggcgggggt taggcgtgga ggattttgta atacgcttcg catacggcag
      301 cgacgactcg ctcgacttcg tctggggtgt tgtatctccc gtaagaaatg cggatcgcgg
      361 actgggcccg ctgctcggat aggcccagcg cgaggaggac gtgggatggg gcgtgtttgc
      421 ccgagacgca agcggcgccc gtggagacgc agacgccctt caagctgagt acgttcatca
      481 acgcttcacc ggatactcct tcaaaaccga gattgattag tccgggtagt cgaggattac
      541 tgtccccgtt gacccatact gtcgggattt tcgcttttat accgtctacg gtatcctcaa
      601 ccattttccg caaccgtttc gcttcggagt ccatcacgcc aaagctttct tcgagcgcgt
      661 aaccggccga aacgacaccg gcgacgtttt cggttccagc gcgtagccca cgttcttgtt
      721 cgccgccggt caataaagga gctagttcta tttccgcgcg tttatacagt atccctgcgc
      781 ctttcgctcc attgaacttg tgagcggatg cggtgagaaa atccaccgga aggcgggaaa
      841 ggtcaacggg gatatgcccg accgcttgca cggcgtccgt atgaaataga acgcccctat
      901 ttctcagcaa cttccctatc gcatcaaccg gctggaccgt gccgatctca ttgttggcga
      961 gcataatgga aaccaatctg gtgtgcggtt ttatgacgtt ttccagggcg gtgagatcaa
     1021 ccattccctt ctcattgaca ggcacgtaag tgatctcgac ccccgatctt tcgagggctc
     1081 ggcacgtatc caacacggaa tggtgttcga tttctgacgt caccatatgc gtcggcttac
     1141 cgcgataggc gttcgctata gctcgaatca cccaattgtt cgcttccgtg cctccggagg
     1201 taaagaaaat ctctgtttct tccgccccta tcgcttccgc cacttgccga cgcgcctggg
     1261 tgatcgcccg tctcgccttc acgccgaatg aatgctggct agaggcgttt ccatattccc
     1321 cttgcagaaa tgggagcatc ttttctaaaa cggtatcggg tattttcgtc gtcgcggcat
     1381 tatccgcata aatcacggtc tcgccctcac tcgattgtcg gaatctatga aaccggatat
     1441 ccgtattttt gaatgtcttc ttagccgaaa agttcgcaac cggcgtcttt gaactcttga
     1501 atgcgctcct ccaaatcctc gacggctatc tcgaggtgtt cggccaagca tcgcatacag
     1561 agaaggtttc tttgctcgcg gccaatgagt tttatgttga gtcctacttc gtcgcgtgtc
     1621 aaatccttct tgccacaaat gtagcagttc gcttttctta tcgccttcac gtcttaactt
     1681 tctgacgatt ccctaacggt gaacgcaggg atgctctttc ccttgtactc gctgtccaat
     1741 aggcctagtt gaatttttat tagatgtcgc atctgcattg cgggtcttaa acaataacgt
     1801 cggaattcga tcggttcgat atcttccggt gcgcgtacat tcggaaatag tagtttcaag
     1861 taggctgtcg caattctttt taccgcttcg gtgtcgcgag tgtccgccgc ttcgggtacg
     1921 acgatcaacg cgtcaactat tgcgcggtag ctcggatcgt cgcgtaactc gtgcaaaatg
     1981 gaacaaaaat actctgaatt caacgcccaa ccggagatct tcatatcatc gttcatacga
     2041 ggaattttcc atccttcgat aaagccgtgg aagcggtcta tgagcgcgga ttcgtggaaa
     2101 acgggcggta gttcctcgaa catactttcg tactcgctca ttttatccct ggggatgtta
     2161 ccgcacagta cgatgccagc gtcggaagtg ccttccactt ttcccacggt gtaaacgccg
     2221 ttttccatat agcctttgag ggcggcgcgc atttcgtcca gaggatggaa gttaatggtc
     2281 tgcacctcgt cgaagacgat gaaatcgtgg ccggcaatta acccgtgctc tttcctctgg
     2341 atgtcgtaga acaacttggc gcgggtcatc aatccgccgg aggatagcca cccgaaccga
     2401 ctgaccctac cgaaaaggta cgactttccg gtgcctttcg gggccagctc tactaaattg
     2461 agccttttct ccacaaaagg tagaaggcgc gtgagcatcg tgagttttgc gtcgtcgttc
     2521 gcatacccat ccgcattata gtctatcgcg cccagcagca catcgatcca ttctttggtc
     2581 gtgaactcgt tccgggtgtc tttgtaataa tccacatcca ctacgtacgg gcaaaaattc
     2641 ttgaaacttc tcaattgaat tttgcctttg cgtttgagtc cgtcgtcggg ctccaagtac
     2701 tccagctcga cgagacccca tatttcacgg ctttttgtca gatcgttgga gcacgtgaac
     2761 cagacttccg gcggtatacg cgtctccttc gtcgtcaaac cgaagtccgg aaggctaaag
     2821 gaaacccctc cggttttgat atccacatcg gcagacacct tcgccaaaaa acgcacacgt
     2881 tccatttcgg tgacgatctt actcttgatc gcagtccaat cgtcttttcg cgggatattc
     2941 ttccgaatga aggcaagcat ctcctcttgg tcgaactccc cggtctctaa cataaatttc
     3001 ttgagtaacc aatcccgcat gaaggagggc aaagaaaggc tggcaaagaa gttgctcttt
     3061 tttaggtctt tgaagaccac catctcttcg aagcaactcc gtaatttatc gatcatttcg
     3121 cacgctcctt aaaaatcatc tcgtacttcg atgcctttgc tcttgattgt gaagtccttg
     3181 ctgacaatat agttttgatt ttcataaatt tccatcttgt attgcccttt cttcaacccc
     3241 tgcaggacaa ccctgaagcg gctcttttct agcttctcag ctttataatt ggttccgttc
     3301 acccgagtca taacgttatc gcagtctggg gtaataatca agatgagctc agccgctttt
     3361 ttatgactca cttccgccac gttattttcg aaagtgactt gaatacttct agtctctaat
     3421 gtcagcatga ttattggtac gagaatctct tctagcgtcg cgcctccgtg aacctcgacg
     3481 ctcgcggatc tcgaacctct gaaccgtgaa taatccgcca cgatcgcgta atcgttttct
     3541 ttggtgatgt tgtctccctt gggtaatacg gctccttggc aataccgccc gctcgatttc
     3601 gcttcgcatc ctccactatc gattatgata tccgggctga tcaccgccag tctgctggct
     3661 ccgtgatcag acgcgattat tactgtgtcg aaatcgcctg ctcgcagttt taccgccgct
     3721 cgctcgatgg cttcacgaac ggcgtcgagt tcttccggca agtgtatcgg gtaaggcgac
     3781 ttgtgaaaat catacccacg ctcgggatta tgtttcaggt catcgatggt cttaatggga
     3841 ttttcttttt ggccttgcca ctcatcgtaa aactccctgt tcacgcccgt gatcgttggt
     3901 aaattcgccc tcgcgattgt cggatcgtat ttcaatttga attccgcgca tagtttttgg
     3961 atatacccca aatactctac tcccagagca tccaaaaaaa tgagcagggt acgatccgtt
     4021 ttgatgtctg aaaaaaccgt tgaacgggtc gggagggaat tgtaaggacg tttcgctccg
     4081 tatactttta ctaattccgc gaacgatgca tcgactgtgt tatacacctt gcatttctta
     4141 taggcggaga aataggccgt caaactctca tcgacgatcc gataatcttg taaataggcg
     4201 aacaacgcgg gatagctcgt ttgaagatag tcacccttcg cgccctcccc caaggcgacg
     4261 agaacctctt gtttctcgcg tttcgtgtta tcggtcaaat aggcgatccg atccgtgccg
     4321 tggcgatgga tcagcgggat gaaatttgcg atgtccgcgt cactacagcc tttcaacaac
     4381 gttttcctct gttcgtaaaa aacagggaac cgactgtccg atagaccaat gtcgagaata
     4441 gcgtttttcg cggtctctag caaatcgtcc gaggttgtcg ttttggaaac cacgaaacct
     4501 aaataatctc ggctgtttgc gaagattttt agccaaatga aatagagcca ggatacataa
     4561 tcgttcgatt tcgcatattt caagaagcat agctctgggg gcgaaaaacg gtggccttct
     4621 aaataacgac ggaagtcctt ctcgttccca tcgaacgcag cgagcaactt cctccagtat
     4681 tcgggggttc cgttagctgg cgataggttc ttgaatacag ccgggtacct gttattcaat
     4741 tcggcgaaat gattttcggg atctatcacc gtaacattcg cgattcgtag cgctgtcttc
     4801 acctttccgg gtggattccc catttcacaa gcctttaaat aggcttttat accgtaaacc
     4861 acctcatcct cgttgacaac aaaattctga gggacatcct ccgggaggac gactcgagat
     4921 accgtccgtg ggtcttcctt tatgatatct ttgagcacgg ggtacataga ggcgcctaac
     4981 agtatgacca cgtgactctt gggctgcaaa gtcatcgctt tgtaccgagt taggtatttc
     5041 tttgcttcgc cagctttgct gactaagtag tcgccaaagc ctaggagcaa ggagttcccc
     5101 tggctcgatc ggacgtcttt ttctagtttt gcgatatccg gttctttatc agccccaaca
     5161 caatagccgc tgacgtagat tattttttcc tgctgatttg cttgaagtac ctcttggtac
     5221 tcctccggtg aaaccacgac taaaagggga aattgagacg cgctgccgaa gtactgcttt
     5281 atttttgcta ttgtgtcgcg cattatgatc ccacgccgcc gtcttccaag atctcgatac
     5341 cgacggtcat attgccttca acgagacgtt tcaagtattc tttaagttta tcggaatcca
     5401 tactgtctat gcggcgtatc actctctgaa aaggctcctg gctgtatttc ttttgagcca
     5461 attcctgaat cagccagttg acatctgggt gtccgctcca atcgtaaggg ctgacgcgcg
     5521 tacgtttact caaatgctct cgaacttcgt ttaggtcggt caagacggcc ttataccgcc
     5581 cgaggatagc gtcttggaaa gccctgtcga cggtttcttg attgcggaag ttttcccagt
     5641 aagagatgcc cgtcaaaaat tccagggcga atttaacctc actttctgtg gagtgattga
     5701 aactgaccgc gtcgaaggaa cgtttaaaat cgtcccattt gcccgtcggc acacaggcga
     5761 gtatgggagt gcggtagcgt gttgaccatt cgcgtgggtc gtcggtaccg gtcttctcct
     5821 tccatagttg gcgaagctgc aaccgcgcga ggttgctttg aaagtcgcta acgatttcgc
     5881 caaccttcga aataaagtct ctctcattca tttcgaaaca attgatcggt atttttccgt
     5941 aaatgtctga gatctctgct tccgaaaaac cctgcagctg gaacgcgcag gcgtgtgtga
     6001 acgcttcctt gtcgtttttt aaaaagctca cgatgctata gccatatttg actataacat
     6061 cgtaaaacgc cttaataccg tcggtaccga tactctttgc cacgttgtac aacgcaggcg
     6121 cgaaagacgc gtattcgggc aactttttaa cgacctcgtg ggagactttg acgaacttta
     6181 gcttgtcgcg ccacgcctca aatgatgccc cgacggtctg ggtgtccttc gctaaaagct
     6241 cgttgctctt tttgataaac tcgtattcca aaatcacctt gcggatttgc ccttcaccgg
     6301 tttcttcgtt ccaaagccaa agcccctcgg tgtcattgcc gaaatgacgt ttgatatcac
     6361 tgaccacacg gtcatcggaa acacggattt cttttgccag ctgcctgagt ttcccagctt
     6421 cgaaacccgc gaggaaatcg atcaacgcgg aatcgaagtt ctctgttttg atgagcgctt
     6481 tcagcttgtt cgcgagggcg ttatcgtgca ttacgaattt tcctatattg ccgctg
//
LOCUS       DAOPXN010000127         6492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11826_length_6492_cov_4.908653, whole genome shotgun sequence.
ACCESSION   DAOPXN010000127 DAOPXN010000000
VERSION     DAOPXN010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_6492_cov_4.908653"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..754
                     /locus_tag="PKM49_07395"
     CDS             482..754
                     /locus_tag="PKM49_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNY82673.1"
                     /translation="MNKKELVAKLAERTGETQKVSEKMVNAFVKVVEDELAQGGEVKL
                     IGFGTFKVSQRKARTGVNPKTMKKMKIPAKKTPKFVPGKELKEKVL"
     gene            1175..2758
                     /locus_tag="PKM49_07400"
     CDS             1175..2758
                     /locus_tag="PKM49_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82674.1"
                     /translation="MNRRFTKFFAILCLGLLLFSFGFAKTNVPNDTVVIAADTGIFIT
                     MDPGMVYEVLPGKIVDALYAKIVELEAHGDTLVPVPRLAEKWEISEDAMTYTFTMRKG
                     LKFANGDPLNANDAVWSIKRSMKLTSSSKWLIESVGITEDNVDQTVKLIDDYTFTMKF
                     DKPYASNIILGILTNQFASVVNRKQVEPHMSGDDLGQKWLDDNSAGAGAYVLQKWERN
                     NQIVLVANPYYVEGQPKIRRIVVRDVPEAANQRLMVEKGDADVAWNVTGTMLKEMAAN
                     KELRIVRVPGHGNEYLAMNASWGPLKDPKVRLAVKYSINYDEIIDILLDGNALKVQGF
                     VSKGYFGYVADNPFYQDIPKAKKLLAEAGYPNGFEVELMTSTANPRPAQAEKIQADLA
                     KVGIKANIVIVQASQMYTKYRERGQQMVIAGWGNDYPDADNLAHAHANYKANQLAWRN
                     AWEDEFAANLTIEAQFEKDPVRRELMYKILTDYWFLNGPFAMLYQTVETWPVRNELKG
                     FEQAAIGYGMLFDFTKLYK"
     gene            2876..3886
                     /locus_tag="PKM49_07405"
     CDS             2876..3886
                     /locus_tag="PKM49_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82675.1"
                     /translation="MNIWKYLIKRILLIALVLFGVACIVFYIAHVIPADPVGAILGGN
                     APPEKIDKLRAQLGYDRPLIEQFFSFLKGAVVGDFGISIKTNNPVMKDIGLYFPATIE
                     LALVAIVISIVLGVLLGILSAVYRNRLIDNVSRVFSILGVSMPVFWIGLILILIFYYK
                     LDWLPGGGRLDFFVDPPRRITGMYLFDSLFTGNWEAFRDALIHILMPAFVLGYNSTAS
                     IARITRSSMLEVLGQEYIRTAKAKGLKKRLVIYRHALRNALIPTITIIGLVFGGLLEG
                     AVLTETVFSWPGLGRYITAGLLSLDYPAVMGGTIYIAFIYSLANLTVDILYALLDPRM
                     RT"
     gene            3891..4781
                     /locus_tag="PKM49_07410"
     CDS             3891..4781
                     /locus_tag="PKM49_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82676.1"
                     /translation="MATEKTSLSFLERNKPYIDDLKHTLFLFRKSKLTMVGFILIVIF
                     LLVAFFGPLFAPYDPIEQNFPARLQGPSREHLFGTDQFGRDIFSRVIHGSRMALWIIF
                     LVSAISGILGTTVGIIAGYFGGLIDDIIMRITDMFLAFPSLVLAMAFAAMMGPNFTNT
                     IIAISLVNWTTYARLARAEAMKVKEQPYIEAIRSVGAGTGRILFLHVLPMCVSPVIVQ
                     LTLRMGTIILTAAGLGFLGLGVQPPTPEWGAMVSVGRNFLVDQWWISTFPGFAIVFVV
                     LGFNLLGDGIRDMLDPRLRR"
     gene            4785..5795
                     /locus_tag="PKM49_07415"
     CDS             4785..5795
                     /locus_tag="PKM49_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82677.1"
                     /translation="MDLRGNLLDIRELRVTFHTYRGVIKALNGVNLWMDKRERLGIVG
                     ETGCGKSVTAQSVMRLIEEPGKITHGEVWFETRNLLALSEDEMTKVRGREISMIFQEP
                     KSSLNPVFKSGFQIKETLLNAKKATPATAEAEVEKILGYVGMDAARVKNMYPHELSGG
                     MAQRVMIAMALATQPKLLIADEPTSALDVTIQAQILELLSDLTHKMGNAVMMITHDMG
                     IVAEFCDRVVVMYAGNAIEIAATKELFRNPTHPYTQGLLRAVPRVGRPGELKAIPGTV
                     PDLVDPPEGCRFHPRCDYATEGCKKELPPLLEIEPGHFVSCYLCQDGGCKPVKKGEEE
                     PW"
     gene            5789..>6492
                     /locus_tag="PKM49_07420"
     CDS             5789..>6492
                     /locus_tag="PKM49_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HNY82678.1"
                     /translation="MVETLMTITNLKKHFLLKQNLFAKNKIVKAVDDVNLEIPRESIY
                     AIVGESGSGKTTLGRTILRLIPPTAGSVKIEGKDVLTLDKKEFMPYRRKMQIVFQDPY
                     NSLHPRKLVKTVIGEGLKIHFKGISEMEIRDKIREVLVQVGLREEHMFRYPHEFSGGQ
                     RQRIAFARAIVLEPEFIVLDEPTSALDVSVQAMVLKMLQEAPAQNALTYLFITHNLSI
                     VDYFANHLAVMYLGRVV"
BASE COUNT         1512 a         1956 c         1747 g         1277 t
ORIGIN      
        1 agcttttttt cctttgtgtt ctctctgtgt cctctgtgtt ctctgtgcga accctgtttt
       61 ttaagacgtg tacaaatgcg tatcgcgccg tcgcgagaag aaggggcgct tacgccgcga
      121 tgcctgttta ctccgtgctc tctgtgttct ctgtgcgaac cctgtttttt aagacgtgta
      181 caaatgcgta tcgcgccgtc gcgagaagaa ggggcgctta cgccgcgatg cctgtttact
      241 ccgtgctctc tgtgttctct gtgcgaaacc atcttttggt tttgtaaagc tatgccaact
      301 taaaagttca gcgttcccag tcgctcccag aaagttagcc cctcagttga aggggctttt
      361 ttttgcaaaa ggatgtgcta taataactga taccgtctaa cccagagccg attcacccta
      421 cgtaggataa gcccaaatat cgcgataaat aacacaactt cattactagg agggggagcc
      481 tatgaacaag aaagagctcg ttgcgaaatt agccgaaaga acaggggaaa cgcaaaaagt
      541 gtccgaaaag atggtcaacg ctttcgttaa ggtcgtcgaa gacgaattgg ctcagggcgg
      601 tgaggtcaaa ctcatcgggt ttgggacctt caaagtctcg caacgcaagg cgcgcactgg
      661 cgtgaatccg aagactatga aaaaaatgaa aatccccgcg aagaaaacgc cgaagttcgt
      721 tcccggtaaa gaactgaaag aaaaggtcct ataaccccgt taacagcttg taccagaggg
      781 cgcctttcgg gcacgaacgt cgtagagtga tacgagtcct cgcaacagcc gtttcacccg
      841 aacgccgcag aatgcaaccg tcaaaggcca ccagacagcg ggttccgggc gtaaacgccg
      901 tagtgtgatt ctcgccctgc cgcacgccgt acaccgagcg tcgccaaacg ccatcgttag
      961 agtcacgcca gtacgagtct gtataagtca gacgccagta ccatcgtcag aagtttacag
     1021 aaccacgttt tccaccaagc aacaccggtt catcttggtt tcttgtcatt tcttttctat
     1081 gtgtgaagag gcgggtattc gtctcttctt ttctattcgc cgtctttaac actaccagtc
     1141 tccatcgaac cagagattat tgggaggtgc acgtatgaac agaaggttca ccaagttttt
     1201 cgccattctc tgccttgggt tactgctatt ctctttcggc ttcgcgaaga cgaacgtacc
     1261 caatgacacc gtcgtgatcg cggccgacac cgggattttc atcacgatgg accccggcat
     1321 ggtctatgaa gtgctcccgg gcaagatcgt cgacgccctg tacgcgaaga tcgtcgagct
     1381 ggaagctcac ggcgacacgc tcgtccccgt cccgcgcctg gccgaaaagt gggagatctc
     1441 cgaagacgct atgacctaca ccttcacgat gcgcaaagga ctcaagttcg ccaacggaga
     1501 cccgttgaac gcgaacgacg ccgtctggtc catcaagcgc tcgatgaaat tgacctcttc
     1561 ttcgaagtgg ctcatcgaga gcgtcgggat caccgaagac aatgtggatc aaaccgtaaa
     1621 actgatcgac gactacacct tcacgatgaa atttgacaag ccctacgcgt ccaacatcat
     1681 cctcggcatc ctgacgaacc aattcgcctc cgtggtcaac cggaagcaag tcgagcccca
     1741 tatgtccggt gacgacctcg gacagaaatg gctggacgac aactccgccg gcgcgggcgc
     1801 ctacgtgctc cagaaatggg agcgaaacaa ccagatcgtg ctcgtggcga acccctacta
     1861 cgtggagggc caaccgaaga tccgcagaat cgtcgtccga gacgttccgg aggccgcgaa
     1921 ccagcgcctg atggtcgaaa agggcgacgc cgacgtcgcc tggaacgtca ccggcacgat
     1981 gttgaaagag atggccgcga acaaagagct ccggatcgtc cgagtccccg gccacgggaa
     2041 cgaatacctc gcgatgaacg cctcctgggg tccgctaaaa gacccgaagg tacgcctcgc
     2101 cgtgaagtac tcgatcaact acgacgagat catcgacatc ctcctcgacg gcaacgcgtt
     2161 gaaggtgcaa gggttcgtca gcaagggata cttcggctat gtcgccgaca acccattcta
     2221 ccaagacatc ccgaaggcga agaagctgct cgccgaagcc ggttacccga acggattcga
     2281 ggtcgagctg atgaccagca ccgcgaaccc gagaccggcc caagctgaga aaatccaagc
     2341 cgacctcgcg aaagtcggaa tcaaggccaa catcgtgatc gtccaggctt ctcagatgta
     2401 caccaaatac cgcgaacgcg gccagcagat ggtcatcgcc ggttggggga acgactaccc
     2461 cgacgcggac aacctcgccc acgcccacgc gaactacaag gcgaatcaac tcgcctggag
     2521 aaacgcttgg gaagacgaat tcgccgcgaa tctcaccatc gaggcgcagt tcgaaaagga
     2581 tcccgtccga cgcgaactga tgtacaagat cctgaccgac tactggttcc tgaatggtcc
     2641 cttcgcgatg ctctaccaga ccgtagagac ctggccggta cgcaacgagc tcaaaggatt
     2701 tgagcaggcc gcgatcggct acggaatgct cttcgatttc accaaactgt acaaataaga
     2761 aacagttctc aatcgcctta tcggaacccc cctttcgacc cgttaggcac tatttgggaa
     2821 aaggggggtt tttctaacat aaggaaacct tatcatccaa tcagggggaa ccgttttgaa
     2881 catatggaaa tatctgataa agagaatcct cctcatcgcg ttggtcctct ttggcgtggc
     2941 gtgcatcgtc ttctacatcg cgcacgtcat accggccgac cccgtggggg cgattctggg
     3001 ggggaacgct cctcccgaaa aaatcgacaa gctccgggcg caactcgggt acgaccgccc
     3061 gctcatcgaa cagttctttt ccttccttaa aggggcagtc gtgggagact tcggcatctc
     3121 catcaagacc aacaatccgg tgatgaaaga catcggcctc tacttcccgg caacgatcga
     3181 actcgccctg gtcgccatcg tcatctccat cgtactgggg gtgctgctgg gcatcctgtc
     3241 cgctgtatac cggaaccggt tgatcgacaa cgtatcccgt gtcttctcca tactcggcgt
     3301 ctcgatgccg gtcttctgga tcgggctgat cctcatcctc atcttctact acaaactcga
     3361 ttggctcccc gggggcggtc ggctcgactt cttcgtggat ccaccccgac ggatcaccgg
     3421 gatgtacctg ttcgactcgc tgttcacagg gaactgggag gccttccgag acgcgctgat
     3481 ccacatcctc atgccggcct tcgtccttgg gtacaattcc accgcgtcca tcgctcggat
     3541 cacgcgctcc agcatgctgg aggtactcgg gcaagaatac atccggacgg cgaaggcgaa
     3601 ggggctcaaa aagcggctcg tgatatacag gcacgcgctg cgcaacgccc tgatcccgac
     3661 gatcaccatc atcgggctcg tcttcggcgg cttgttggag ggggcggtgc tcaccgaaac
     3721 ggtcttttcc tggccgggac tggggcgata catcacggcc gggctactct ccttagacta
     3781 tccggcggtg atgggcggca cgatctacat cgccttcatc tactcgctgg cgaacctcac
     3841 ggtcgacata ctctacgcgc tgttagaccc caggatgagg acgtgatgct gtggcaacgg
     3901 aaaaaacctc attgagcttc ttggaaagaa acaaacctta tatagacgac ctcaaacaca
     3961 cgctctttct cttccgaaag agcaagctga cgatggtcgg gttcatcctg atcgtcatct
     4021 tcctactggt cgccttcttc gggccgctct tcgcgcccta cgacccgatc gagcaaaact
     4081 tccccgcgcg actccagggg ccctcgcgag aacacctctt cggcaccgac cagttcggcc
     4141 gcgacatctt cagccgtgtg atccacggtt cacgaatggc tttgtggatc atcttcctcg
     4201 tctccgcgat cagcggcata ctcggcacga cggtcggcat catcgccgga tacttcggcg
     4261 gactgatcga cgacatcatc atgcgcatca ccgacatgtt cctcgccttc cccagcctcg
     4321 tgctggcgat ggccttcgcg gcgatgatgg ggcccaactt caccaacacc atcatcgcga
     4381 tctcgctcgt gaactggacc acctacgcgc gactcgcgcg ggcggaggcg atgaaggtga
     4441 aagagcagcc ctacatcgag gccatccgct ccgtaggcgc cggaacggga cgcatcctct
     4501 tcctccacgt cctcccgatg tgcgtctccc cggtcatcgt gcagctgacg ctccggatgg
     4561 gcacgatcat cctgacggcc gccggcttgg gatttttagg tttgggcgtc caacccccga
     4621 cgccggaatg gggcgcgatg gtgagcgtcg ggcgcaactt tctcgtcgac caatggtgga
     4681 tctccacctt cccgggattc gcgatcgtct tcgtcgttct cggattcaac ctgctcggag
     4741 acggcatacg cgacatgctc gacccgaggc tgaggaggta agcgatggac ctacgcggga
     4801 acctattgga catacgcgaa ctacgcgtca ccttccacac ctaccgcggc gtgatcaagg
     4861 cgctcaacgg ggtgaacctc tggatggaca aacgcgaacg cctgggcatc gtgggagaga
     4921 ccgggtgcgg caagtccgtg accgcccaat cggtgatgcg gctgatcgaa gagcccggga
     4981 agatcaccca cggcgaggtc tggtttgaaa cccggaacct gctcgccctc tccgaagacg
     5041 agatgaccaa agtgcgcggt cgggagatct cgatgatctt tcaagagccg aaatcctctc
     5101 tgaacccggt gttcaaatcg ggcttccaga tcaaggaaac cctactcaac gcgaaaaagg
     5161 cgacacccgc caccgcggag gcggaggtcg agaagatact cgggtacgtg ggcatggacg
     5221 ccgcgcgagt gaagaacatg tacccgcacg aactcagcgg cggcatggcc cagcgtgtga
     5281 tgatcgcgat ggcgttggcg acgcagccga aactcctcat cgccgacgaa cccacctccg
     5341 cgctcgacgt gacgatacaa gcgcaaatcc tcgaactcct ctcggacctg acccacaaga
     5401 tgggaaacgc ggtgatgatg attacccacg acatgggcat cgtcgcggaa ttttgcgacc
     5461 gagtcgtcgt gatgtacgcc ggaaacgcca tagagatcgc ggccacgaaa gagctgttcc
     5521 gaaacccgac gcacccgtac acccaagggc tgctgcgcgc ggtcccgagg gtaggcagac
     5581 cgggcgagct caaagcgatc ccgggaacgg tgcccgacct cgtcgacccg ccggagggct
     5641 gccgattcca cccgcggtgc gactacgcca ccgaaggctg caaaaaagaa ctcccgccgc
     5701 tactggagat cgaaccgggc catttcgtct cctgctacct ttgccaggat ggcggctgca
     5761 aaccggtgaa aaagggagag gaagaaccgt ggtagagacg ctaatgacca taacgaacct
     5821 caaaaaacac ttcttgctga aacaaaacct cttcgcgaaa aacaagatcg tgaaagcggt
     5881 agacgacgtc aacctcgaga taccgcgcga atcgatctac gcgatagtcg gggagtcggg
     5941 gtccggaaag accaccttgg ggcgcacgat actccggctc ataccgccga cggccggctc
     6001 tgtgaaaatc gaaggaaaag acgttctcac cttggacaaa aaggaattca tgccctacag
     6061 gcgcaagatg caaatcgtct tccaagaccc ttacaactcc ctacaccccc ggaaactcgt
     6121 caagaccgtg attggagaag ggctgaaaat ccacttcaaa ggcatctccg agatggagat
     6181 ccgcgacaag atacgggaag tcctcgtaca agtggggcta cgggaagaac acatgttccg
     6241 atacccgcac gaattctccg gcgggcagcg gcagcgcatc gccttcgcgc gcgcgatcgt
     6301 cctcgagccg gagttcatcg tcctcgacga acccacgtcc gcgctcgacg tttccgtaca
     6361 ggcgatggtc ctcaagatgc tgcaagaggc gccggcgcaa aacgcgctga cctacctgtt
     6421 tatcacgcat aacctctcga tcgtcgacta cttcgcgaac cacttggcgg tcatgtacct
     6481 cggacgcgtg gt
//
LOCUS       DAOPXN010000128         6431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11992_length_6431_cov_5.803795, whole genome shotgun sequence.
ACCESSION   DAOPXN010000128 DAOPXN010000000
VERSION     DAOPXN010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11992_length_6431_cov_5.803795"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            324..914
                     /locus_tag="PKM49_07425"
     CDS             324..914
                     /locus_tag="PKM49_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HNY82679.1"
                     /translation="MTSFRGRYGEAFKFLVQGTDTGKFWGTEIYTDDSRLSLAAVHSG
                     SLQIGEYGIVEVTVLPGQDHYTGSAQNGVTSEDYGAWPGSYSLRRVSEETIIGIGQKD
                     PGDLTPYRERTDAVLRFSVTGSDWGSVWGSGVYTDDSTLAMVCVHAGLLRIGETGLIE
                     VTLLPGLEEYEGSTQNGITSQSYGSWQWSYSVTRIL"
     gene            1082..2080
                     /locus_tag="PKM49_07430"
     CDS             1082..2080
                     /locus_tag="PKM49_07430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82680.1"
                     /translation="MNRTAQDESGSPVRLMACVPAYNNPFDLERLLDSIVDSTRKVDL
                     VFIVDNSEDGYLERNQTLVARYPDNFVYYPNRKPLKGCAQAFRIGMEKFLELDYDLLL
                     LLDQDGLISPDSIEKYIEHRHEADLLVPRIRNIDPNSPKKLGLLPSKVTGFAKTYTTE
                     AEPGESVIGPNMGLLINRKVVEHCVYDDENYLVWFSDFDYGLKTRSLGYQSRYMPDIV
                     VYHPNLSEKYPSRSGKLSTLRFRLTPFQHLGYVSQGSTEQETFAVYSRVYMSAKYFHP
                     LVVYANLLYTLNPILLWYRIFRYRVLWRETLSVYRRAIHAGRRDRRSPAKRSGNFG"
     gene            2166..3305
                     /locus_tag="PKM49_07435"
     CDS             2166..3305
                     /locus_tag="PKM49_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HNY82681.1"
                     /translation="MRIALNATNIFHSAEVCGVGQYVFNLLNGFTQLGFLDSFRFIAN
                     SEVAKVLSRRFPEAFITSAPYPPFLDRFGWKDHFFRQFAIERRSVPSFFRREEANLLF
                     HPFMAQHLFVSKAIKTVVTTHDLYLRRFPEQLSKKYYLMLDRSYRKTLLGATRIVTPS
                     NVVKNDILTYYPEVSKEKITVIPNPIEIQTDQKAPFSVPEPYILSVNAFRYHKNLITL
                     IKAFERIHSQIAHSLVLTGPTIPSISTGIFRYIEEKRIPKIIITGYISDAQRNYLYQN
                     ATLFVSPSLCEGFGITPIEAALFGVPVITTRETSIPEVTQNLLHYYEPATDAIRLAEK
                     ILEVLREEPDPVRMQSIQETFRNAYSPSTVARRYWECFQNTLEAD"
     gene            3307..4266
                     /locus_tag="PKM49_07440"
     CDS             3307..4266
                     /locus_tag="PKM49_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HNY82682.1"
                     /translation="MSDSKFEMLRRIYRKCVPLPIRVYFERARNKYGEKVLIYRINRY
                     YEGHQEEKRLLQTELDFINRVKSIQMIPYPFIFEYEPSKIGVYADSVTGFPYVLHRER
                     RIYFPSKSTPDQVRRMFNSLLLEQHVSSPHRYFEDGFYPTEDDVFLDIGAAEGLISLE
                     WVETVRAVILFEPDPIWREPLALTFAPWKEKVTILSKAASSFDQDGCQTVDSVLKHQN
                     GPFLLKLDVEGEEVEVLKGASETLLKPDTRVVCCTYHREDDADNFLRFFSERGYRTEI
                     PRGYLLPTVKAMQNPPYFRRGVIRAWKTNPNEGQTHQNQASEA"
     gene            4269..5339
                     /locus_tag="PKM49_07445"
     CDS             4269..5339
                     /locus_tag="PKM49_07445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNY82683.1"
                     /translation="MKQEAATISIIIPLYNAERTIERCLGSVTRQTYSNLEIILVDDG
                     STDRSPDICDRYAEKDTRIRVAHQPNAGVGAARNAGMSVMHGDYLFFLGADDYIFPET
                     IATLYAEARSTESGITIGNARVSTGNDRFSEVPSFSYQSVTAEKQAIPQVRYELFYDP
                     GYGVPVWNKLYRSGFLKRYAITFERLPTLSEDRLFNTQCFIRHPVIQLVNRYTYVYCA
                     EASTIMRSKIQAPIEKSLRVVKSMQDSFESIQGLSEFEDLLAWLVFGLINQVARNFTI
                     HHDHAFKEIRDFLVAYKQNETVSRCLRAMASGKYLEGIARPSWVRYARYLSRMLNRGA
                     YTAAASLLFLRFKIEARRRRIE"
     gene            complement(6135..6251)
                     /gene="rrf"
                     /locus_tag="PKM49_07450"
     rRNA            complement(6135..6251)
                     /gene="rrf"
                     /locus_tag="PKM49_07450"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1666 a         1547 c         1616 g         1602 t
ORIGIN      
        1 tctttaagac atgccaactt aaaagttggc gttcccaagc gtatcgcgcc gtcgcataaa
       61 gaaaaagcgc gttcgccgcg atgactatat ggacaaaaaa tccgtgcgat cgattctggc
      121 cttgtttctt aattacaccc cgtaacttga cacacacgcc ctcgccggaa aacgatcgct
      181 cgtgatataa tccaaaaaag aatattagtt aattggttgc tcgtttcggc gagggtcagt
      241 gggtactctc ggaagatggt atacgaatat ggagtcgtat atgggcgggt aacaaacgga
      301 acgcttaaaa gtgaggcgtc gatatgacca gtttccgagg acgatacgga gaagccttca
      361 aatttctcgt acaagggacc gacaccggaa agttttgggg aacggagatc tacacggatg
      421 attcccggtt atctctcgca gccgttcatt cggggtctct acaaatcggg gagtatggca
      481 tcgtcgaagt gacggttctt ccaggtcaag atcactatac cggcagcgcc caaaacggcg
      541 ttacttcgga agattacggc gcctggcccg ggagctattc attacgaagg gtgtcagaag
      601 aaacgatcat cggaatcggc caaaaagatc ccggagacct gacgccgtac cgagagcgaa
      661 cggacgctgt tctacgtttt tccgtaaccg gaagcgattg gggatcggtg tgggggagcg
      721 gcgtttatac ggacgattca actttggcga tggtatgtgt gcacgccggg ttattgagaa
      781 tcggtgaaac cgggctgata gaagtaacgc ttctccctgg gctcgaggag tatgaaggta
      841 gtacacagaa cgggatcacg agtcaatcat acggttcatg gcaatggagt tattcggtta
      901 cgagaatcct atgacgttcc ttcctttttc ttcgaataac aaaggcgagc gcgatcggaa
      961 cggtttcaac aatagaaagt ctcgctttga ttctttactc tgatcggtac gtatcaacgg
     1021 ggagagaggg gcatccataa tcgatagcgg aaaggccgtt gaaagagacg attggatttt
     1081 aatgaaccgg accgcgcagg atgaaagcgg gagcccggtg cgtttgatgg cttgcgttcc
     1141 ggcttataat aatccctttg acttagagcg gctgttggat tctattgttg attcaacccg
     1201 aaaggtcgat ctcgttttta tcgttgacaa tagcgaagac ggatatcttg agcgcaacca
     1261 gactttagtc gctcgctatc cggacaattt cgtatactat ccgaatcgaa aaccgctcaa
     1321 gggatgcgcg caggcttttc gaatcggcat ggaaaaattc ctggaactcg attacgactt
     1381 acttttgttg cttgatcagg acggattaat atcccccgat tcgatcgaaa aatacatcga
     1441 acacaggcat gaggcggatc tcctcgtccc tcggatacgc aacatcgacc cgaattcgcc
     1501 gaaaaaactc gggttgttgc cttcgaaggt gaccggtttc gcgaagacct atacaacgga
     1561 ggcggaaccg ggtgaaagtg ttatcgggcc gaatatgggc ctgctcatca atcgaaaggt
     1621 cgttgagcat tgtgtatatg acgatgaaaa ctacctcgtc tggtttagcg atttcgatta
     1681 cgggctaaag acgcggtctt taggttatca atcgaggtat atgcctgata tcgtcgtata
     1741 ccacccgaat ttgtccgaaa aatacccgag tcgaagcgga aagcttagta cattacgatt
     1801 tcgattaacg ccgtttcaac atttaggata cgtcagccaa gggagtacgg agcaggaaac
     1861 cttcgcggtc tattcaaggg tttacatgag cgcgaaatac ttccatcctc tggtcgtgta
     1921 tgcgaatttg ctttatactt tgaaccctat tctcctctgg tatcgtattt tccgataccg
     1981 tgttctgtgg agagagacgt tatctgttta ccgtcgcgcg atacacgccg gtcgccggga
     2041 tcgtcgatct ccggcaaagc ggtcgggcaa tttcggttga gtgccggaga ttatcctctc
     2101 tacacgcctg aagggataaa gaacgaacca atctcgcgat attttaatga cgagggggta
     2161 ggcttttgag gattgccctt aacgcgacga atattttcca ttccgctgaa gtgtgtggtg
     2221 tcgggcaata tgtgtttaat cttttaaacg gttttacgca gctcggtttt ctcgactcgt
     2281 ttcggttcat cgcgaattcc gaggtagcga aagtgttaag ccgaaggttt ccggaagctt
     2341 tcatcacgag tgcgccctat cctccttttt tggatcgatt cggatggaag gaccattttt
     2401 tccgacaatt cgctatcgaa cgccggagtg ttccttcttt cttccgtcga gaggaggcca
     2461 atcttctatt ccatccgttt atggctcagc acctcttcgt ttcgaaggca ataaagacgg
     2521 tggtaacgac acacgatctc tatctacggc gctttcccga acagttatcg aaaaagtatt
     2581 atctgatgct ggatcgttcc taccggaaaa cgttactcgg ggcgacgcgc atcgtgaccc
     2641 cttccaatgt cgtaaaaaac gacattctga cctattatcc tgaagtttcc aaggaaaaga
     2701 taacggtaat cccgaatcca atcgaaatac agacagatca gaaagcgccg ttctccgttc
     2761 cggaaccgta cattctcagc gtcaacgcct tccgttatca caagaatctc ataacgctta
     2821 ttaaagcctt cgaacggatt cattcacaga tcgcgcattc tttagtgctt acagggccta
     2881 cgattccgag catttcgact ggaattttcc gttatattga agaaaagaga attccgaaga
     2941 ttatcatcac aggatatata tccgacgccc agaggaatta tctctaccaa aacgcgactc
     3001 tcttcgtatc tccttcattg tgcgaaggtt ttgggattac cccgatcgaa gccgccctct
     3061 tcggcgttcc ggttattacc acccgtgaaa cgagtattcc ggaggtaacg caaaacctcc
     3121 ttcattatta tgaaccggct accgatgcga tccgtttggc cgaaaaaatc ctggaagttc
     3181 ttcgagaaga accggatccc gttcggatgc aatcgatcca ggaaactttt cgaaacgcct
     3241 attctccctc aaccgttgcg cgacggtatt gggagtgttt ccaaaatacg ctggaggcgg
     3301 attgatgtgt cagactccaa gttcgaaatg ttgagaagaa tctaccgaaa gtgtgtacct
     3361 ctcccgattc gggtatattt cgaaagagcg agaaataaat atggggaaaa ggtcctcatc
     3421 tatcggatta accggtacta cgaaggccat caagaagaaa agcggcttct tcaaacggaa
     3481 ttggatttca tcaatcgcgt gaagtctatt cagatgatcc catacccgtt tattttcgag
     3541 tacgaaccgt cgaagatcgg ggtctacgcc gattctgtca caggattccc gtatgtcttg
     3601 caccgtgaga gaagaatata ttttccctcg aagtcaactc cggatcaggt tcgaagaatg
     3661 tttaacagct tacttctcga acaacacgta tcgagccccc atcggtactt tgaggatggt
     3721 ttttatccga ctgaggacga tgtgtttttg gatattgggg ctgctgaagg gttaatctcc
     3781 ctagaatggg tggaaacggt ccgggcggta atcctttttg aacccgaccc gatctggcga
     3841 gagccccttg cgttgacgtt cgcaccgtgg aaagaaaaag tgaccatcct ctcaaaagcc
     3901 gcatcttctt ttgatcagga tgggtgtcaa accgtcgatt ctgttcttaa acatcaaaac
     3961 ggtccgttcc tacttaaact ggatgtagaa ggagaagagg tcgaagtttt gaaaggcgcg
     4021 tcagagactc tcttgaaacc ggatacgagg gtcgtttgct gtacgtatca tcgtgaagat
     4081 gacgccgata acttcctgcg atttttctcg gagcgaggat atcggacaga aataccgcgc
     4141 ggatatctcc ttccgactgt taaagcaatg cagaatcctc cctattttcg gcgtggggtg
     4201 attcgggcct ggaaaacaaa tccaaacgag ggacagacac atcaaaatca agcgagtgaa
     4261 gcataaagat gaaacaggaa gcggcgacga ttagtatcat catcccccta tataatgcgg
     4321 agcgaacgat cgaacgctgt ctcggcagcg tgactcggca aacgtactcg aatctggaga
     4381 tcatccttgt cgatgacggt tctacggatc gtagccccga tatttgcgat cgatacgccg
     4441 aaaaagatac tcggatccgc gtagcccatc aaccgaacgc aggcgtaggc gctgcgagaa
     4501 acgccgggat gtccgttatg catggggatt atctgttttt cctcggcgcg gatgactata
     4561 tctttccaga aacgatcgcc accttatatg ccgaagcccg gagtaccgaa tcgggcatta
     4621 cgatcggcaa cgcccgcgta tctaccggca acgatcgttt ttcagaagtc ccctcctttt
     4681 cataccaaag tgtgaccgcg gaaaaacaag cgatccctca agttcgatac gaactgtttt
     4741 acgatcccgg ttatggggta cccgtatgga ataagctgta tcgatccggt tttttaaagc
     4801 gatacgccat cactttcgag agactgccaa ccttaagcga ggatcgctta ttcaatacgc
     4861 aatgctttat ccgccacccc gtcattcagt tggtcaaccg atacacctac gtctattgcg
     4921 ccgaggcttc gacaatcatg cgatccaaga ttcaagctcc gatcgagaaa agcctgagag
     4981 tggtcaagtc tatgcaagac tccttcgagt cgattcaggg tttgagtgag tttgaagacc
     5041 tcttggcgtg gctcgttttt gggttgatca accaagtcgc gcgtaacttt acgattcatc
     5101 acgatcatgc cttcaaggag atccgtgatt tcctggtcgc atacaagcaa aacgaaaccg
     5161 ttagccgttg tctgcgggca atggcctcgg ggaaatatct ggaagggatt gcccgtccat
     5221 cctgggtgag atacgccaga tacctatctc ggatgttgaa ccgaggtgcc tatactgccg
     5281 cggcatcgct cttgtttctg cgttttaaga tagaagcaag acgccgaaga attgaatgag
     5341 cgcgacgtaa gtcgggagct ctatgatggg agtccagtaa gttcgtcaga taattgcgct
     5401 tcttgtgcga aaacccggta aagaagaagg cttcgattaa gaaaatggtt tttgtcataa
     5461 gaggtaatcg gttcagtgga agcggcgcga atagtcgtcg cctattcccc atcaagttct
     5521 tacagtagag acgcaccccc gaaaaccgat tccggtaaac ttctatcttt cgatctttct
     5581 cgagttaatt cttccgcgaa acggccactt cgagccatcc aacgactcgg tgcgggtatc
     5641 gttgtgtgga ttaggctgtt tactccatcg cgagagtaca tgatatctgg ccttgcaggg
     5701 acggaacctg gtcgcactac ttccggaaag cacggaggaa tcattctggg aacgccgtag
     5761 ctttggagcg cctaggaccg ccgaacttcg gttcggcaag ttttaggttt ttttgctgag
     5821 aacgccttcc tgggaccgcc aacttttaag ttggcatgtc ttctataagt gtcttgtgaa
     5881 accaacaaaa gccttcgctc acgcagagaa cgcggagtga agatgaaaaa cgcgatgaac
     5941 gaaaatatta acgtgcgttt tctcagagct tctcgaaagc ggtcccagaa aggcacgccg
     6001 aactaaagtt cggcgttccc agaaggcatc cctagaaacc gaggcgaact aaagtgcggc
     6061 gctcccaagg tccggcgctc acaggcaaca aaaaacggag acaaagaata tgtctccgtt
     6121 ctgatgaata aaaccccggg cgataccgac tctcacatgg ggtctccccc atactaccat
     6181 aggccaggag gagcttaacg gccgggttcg ggatgggtcc gggtgggtcc tcctcctgta
     6241 tcgtcaccca gggatagctc tctggaagct gtatagggac tgggcgagaa gaacactcgc
     6301 cacgaagtcg taaaccacga cttccggtaa aatgacgacc actcgtctcg tagtcgaaaa
     6361 tcacgacttc cggcataatg acgaccactc gccacgaagc cgaaaaccac gacttccggc
     6421 taaacgatga t
//
LOCUS       DAOPXN010000129         6429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_11998_length_6429_cov_4.483056, whole genome shotgun sequence.
ACCESSION   DAOPXN010000129 DAOPXN010000000
VERSION     DAOPXN010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11998_length_6429_cov_4.483056"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PKM49_07455"
     CDS             complement(<1..556)
                     /locus_tag="PKM49_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HNY82684.1"
                     /translation="MTGSPYGAHRVIEPAGALPQPAWKICNDMEKVYDNEILIDVSVL
                     NIDSASFRQIKEEAGNDEAKVAEKILSIVNERGKMHNPVTGSGGMLLGKIARISPQMA
                     EKHGVKVGDNVCTLVSLSLTPLKIEKIHKVHLEKEQVEVTAKAILFESGVCAQLPNDM
                     SETLALSILDVAGAPIQTARMVKPG"
     assembly_gap    666..675
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<676..1596)
                     /locus_tag="PKM49_07460"
     CDS             complement(<676..1596)
                     /locus_tag="PKM49_07460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNY82685.1"
                     /translation="MTVRRWIGGLILSVFLITIGYAQKDYGKLSLPSDTVVAKVNGKP
                     IYAKTLDIESNLTYTLLTIKQNNENFYEILLGTEEGFDFLVKYKTIVLYKLIDSFLLI
                     EIAKSWGIHYEDADMLRYVNTYIKDILDQNQLTEAEFENYIVTQGFDSLTDYKEHLAF
                     QRKVTLTNIQLMDKIIAAVPVSDAEVDAYIKSHPLPADETASVNISHILLQKEDNVNL
                     VLSSVQTIGFEKTAELYSRDDLTKHNGGNLGWLEKGAFPQFDVAFGYQVGDVIGPIQT
                     QLGYHLIRINDFSGTRTGQSPYREEAKNLLS"
     gene            complement(1628..4675)
                     /locus_tag="PKM49_07465"
     CDS             complement(1628..4675)
                     /locus_tag="PKM49_07465"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HNY82686.1"
                     /translation="MKKIEMLFLCFTFIFITMFAHPRLTYEFSTPKYFFLVIFAVALM
                     VLFIVRKLMEKDRSLYLAWPHLGYFLFGASAVVASFAMLRENALYFPYPFELGMYALL
                     TVFFSVYLSNFFEEKKEILFFLTAVLIAGFFVAVEALMNYYDGQSFFLGGFGGSGKMQ
                     MKATIGNPNFVSDFLASLIPIAIYFAISNQYGFRNKSDSVDQKRFFFILGIKTFGVVC
                     FFLFYLVVLLAGTRAVYLALMGGFLIFGVLVFWYRKRIFLPSAPRTEVKEKNPKKKTE
                     GKQLSVKPLSLIALGAVVVILLFLPVILSLPGNFLGTRVDALGRVATSFETRGFQITG
                     GKGRLLSWAASIYQWKDYPLTGNGLGTYKLKDGDYLAQVMEEHPEYIDVWNNYKRTHN
                     DYLQVLGEMGLFGFLTMSATILLLIVLFFQMLKRMKNGDDILLFLLFAVCFFEILGHS
                     ATEFPLQMLPNQLWAIVLASVGFGPYFNREKRMAKTVSIKKHLWLAALAAVLGIGLIT
                     GYLKYHSLFAEVFFKDGNTYYSYLSQVDSAQADLANKEQEYLALQGQLEHREGNYAGF
                     NPDVYMQKKLAGQNVSAMSPIALSQLKIKILGDLEKEVDAEKQKLASIFSQIENKKIE
                     YQGLSQTYYLSALDSFEKSIGHLPAFGKSFFYIALIMVRPERKEAKVSEFNQGKDHMS
                     VLSKHFVTDTEEVRHILPEYRDRLLEEDFSVFSGLIKAGVPFQDLVDGFKLSLWYDIQ
                     MNQDSLDYFETSLKVFSEKNTFRIMGKSCLQIISYLRELISAYRVWAERNPEYATSMQ
                     RLIVEHEKQIKTKIVELQTWMDTALYTLPGSWHLFPDWENVYQEYITMLLKLGTPAEY
                     YGKIKEIVEKRIHAVEYTHRAQQLAVPSEISNLYRYMIQHFHDQQKYQEALILYKEVV
                     DMLKDPYEWNRVDLTHNPYLDDSGKAKIQSFLQDYERLEGDFSQYYHTLQNHYQAMND
                     QGMFDQYLLPDWRSNELTGETWEDPTYETVQLRMTRLAETDRQ"
     gene            complement(4742..6334)
                     /gene="lnt"
                     /locus_tag="PKM49_07470"
     CDS             complement(4742..6334)
                     /gene="lnt"
                     /locus_tag="PKM49_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNY82687.1"
                     /translation="MKKRGLWIGAGILMALAMPGFNLHFFVWIGFALFLRSLDRENAP
                     LSARSLIRNTLRGLGFGLVFFGVSLHWFLPTFAHYIPQVLRSFPPIMGAVVFLLLISV
                     EALFYGLFAFVYTLFRSRVTDRPTLFCLFIGLLLFVTEWLRGLGPMGFPGFRFSDALV
                     NMLGLSQLVAFGGTEALVILIGVVNAALFRILSARPFLSSSKRSWVLVALLLLSLYAA
                     DSVTQNLLPPVYPQNGETRWVGGMQTMFRSEDKYTFSEAEFIEEFSDVLSLLDSVPKT
                     PDFVAFPEAQFMYDIRERTKTAKALERLSTEREVVFVVGHLAQTNDEEYYNAVRTVSP
                     EGGVSNDFYGKRHLNPFVETLPFERIFGMLSFIKFSNYFTPGPFAQTFEISGKRYAFP
                     VCFESYYSEVFGDFLAQKAEAFFVLTNDAWFTSPIALEQHFVQARFRALETGKWVGQV
                     SNNGITGVSDPFGRVVARIAPFEKGLGVFLIGFSDQTRAITFLDYRLLCLSGYGALFF
                     LFVAVALTRKRQPEKPQGYEMK"
BASE COUNT         1586 a         1629 c         1633 g         1571 t
ORIGIN      
        1 cgccgggttt gaccatccga gccgtttgaa tcggcgcgcc ggcgacgtct agaatcgaga
       61 gcgcgagggt ttcggacata tcgttaggaa gctgcgcgca cacgccgctc tcaaagagga
      121 ttgccttagc cgtgacctcg acctgttctt tttcgaggtg cactttgtga attttttcga
      181 tcttcaaagg tgtcagcgag agggagacca acgtgcagac gttgtcgccc actttcacgc
      241 cgtgtttttc agccatctgg ggggatatcc gcgcgatctt tccgagtagc attccacccg
      301 agcccgtcac cggattatgc atctttccgc gctcgttcac gatggatagg atcttttcgg
      361 ccactttcgc ctcatcgttt ccggcttctt ctttgatctg tcggaaagaa gccgagtcga
      421 tgttgaggac ggacacatcg atcaaaatct cgttgtcgta gactttttcc atatcattgc
      481 aaatcttcca agcgggttga ggtaacgcgc ccgcaggttc gatcactctg tgcgctccgt
      541 acggtgatcc tgtcacgtca ttctcctcct caatcaatag tatatttcta tgggataggt
      601 aactttcatt tgcgggaact cttccgtgag ccatttctcc cacaactcga cctttttctc
      661 cagggnnnnn nnnnnggaga gtaagttttt cgcttcctcg cggtaagggc tctgtccggt
      721 tcgggtgccg gagaaatcgt tgatacggat gagatggtag ccaagttggg tttgaatcgg
      781 gcctatgacg tctcccacct gatagccgaa agcgacgtcg aattgaggga aggcgccctt
      841 ctccagccag cccaggtttc cgccgttatg cttcgtcagg tcgtctcgag agtacaattc
      901 cgccgttttt tcgaatccaa tggtttgaac cgaagagagg acgaggttga cgttgtcctc
      961 tttctgcaac aaaatatgag agatgttcac cgaggccgtt tcatccgcgg gcaatgggtg
     1021 gctcttgata taggcgtcca cttcggcgtc actgactggg acggcggcga tgatcttgtc
     1081 catcagctgt atattggtga gcgtcacttt tcgctgaaag gccaggtgct ccttatagtc
     1141 cgtgagagaa tcgaagccct gggtcacgat gtagttttca aattccgctt cggtcaattg
     1201 gttttgatcc aaaatatcct tgatataggt gttcacatag cgcaacatat cggcgtcttc
     1261 gtagtggatg ccccaggatt tggcgatctc tatcaacaga aaggagtcga tgagtttgta
     1321 taagacgatc gttttgtatt taactaaaaa atcgaacccc tcttcagtgc ccaggagaat
     1381 ttcgtagaag ttttcgttgt tttgcttgat agtgagcagc gtgtaggtca agttcgattc
     1441 gatatcgagc gttttcgcgt aaatcggttt cccgttgacc ttcgccacaa cagtatctga
     1501 gggtaatgac aattttccat agtctttctg cgcatagcct atcgtgatga gaaacacgga
     1561 gagtatgagg cctccgatcc atcttcttac tgtcatgatt cgctttctcc tttcggaccg
     1621 ttcgtcatca ttgccggtca gtttcggcca atcgcgtcat ccgcaattgg acggtttcat
     1681 aggtcgggtc ttcccaagtt tctccggtta attcgttcga ccgccaatcc ggcaaaagat
     1741 actgatcgaa catcccttgg tcgttcatcg cttgataatg attttgaagg gtgtgatagt
     1801 attgggagaa gtcaccctcc aacctctcgt aatcttgcaa aaagctctga attttggcct
     1861 ttcccgagtc gtccaggtaa ggattatgag ttagatccac tcggttccat tcgtacgggt
     1921 ctttgagcat atcgacgacc tctttataga gtatcaaggc ctcttggtat ttttgctgat
     1981 cgtggaagtg ttggatcata taccggtaaa ggtttgaaat ctccgaggga acggcaagct
     2041 gttgagcccg atgcgtgtat tcgacggcgt gtatccgttt ttcgacgatc tcttttatct
     2101 tcccatagta ctctgccgga gtgccgagct tcaggagcat cgtgatatac tcttggtaca
     2161 cgttttccca atccgggaac agatgccagc ttccggggag agtatacagg gccgtatcca
     2221 tccaggtctg caattcgacg atcttggttt tgatctgctt ctcgtgttcg acgatcagcc
     2281 gctgcatcga tgtggcgtat tcggggttac gctccgccca tactctgtaa gccgatatca
     2341 gctctcgtaa ataactgatg atctgtaaac aggattttcc cataatccgg aaggtgtttt
     2401 tttcggaaaa gaccttaagg ctcgtttcga aatagtccaa agaatcctga ttcatttgga
     2461 tgtcgtacca caaagagagt ttgaacccgt cgacgaggtc ttgaaaagga acacccgcct
     2521 ttatcaaccc gctgaaaacc gagaaatctt cttcgagcag ccgatctcgg tattcgggaa
     2581 ggatatgccg tacctcttcg gtatccgtga caaagtgctt gctcaagacg ctcatatggt
     2641 ccttgccttg gttgaactcc gacaccttgg cttctttgcg ctccggccga accataatca
     2701 gcgcgatgta aaagaagctc ttcccgaagg cgggtaggtg gccgatactc ttttcaaagg
     2761 agtccagcgc cgagagatag taggtttgcg atagcccttg gtattcgatt tttttgtttt
     2821 cgatttgcga gaagatcgac gcgagctttt gcttttctgc gtctacctct ttctccaaat
     2881 cgccgagtat cttgattttc agttggctga gcgcgatcgg gctcatcgcg ctcacgttct
     2941 gaccggccaa ttttttctgc atatagacat ccgggttgaa ccccgcatag ttcccctccc
     3001 tgtgctccag ctgcccttgg agtgccaggt actcctgctc tttgttcgcc aggtccgctt
     3061 gcgcgctatc cacctgagaa aggtaggagt agtaggtgtt gccgtccttg aagaagacct
     3121 ccgcgaaaag ggagtggtac tttaagtacc cggtgatgag gcctatcccc aaaactgccg
     3181 ctaacgcggc cagccaaagg tgttttttga tcgatacggt cttggccatt ctcttttccc
     3241 gattgaaata gggtccgaaa ccaacgctgg ccaaaacgat cgcccaaagc tgatttggaa
     3301 gcatttggag cggaaattcc gtcgcggagt ggcccaggat ttcgaagaaa caaacggcga
     3361 aaagcaggaa taggaggata tcgtctccgt ttttcatccg tttgagcatc tggaagaaaa
     3421 ggacgataag gagcagaatc gtcgcgctca tcgtcaaaaa gccgaaaagc cccatttcgc
     3481 ccaacacctg gaggtagtcg ttgtgggtac gtttgtagtt gttccatacg tcgatatact
     3541 ccggatgttc ttccataacc tgagccaagt aatcgccgtc tttcagttta taggtaccca
     3601 gtccgtttcc ggtcaacggg tagtctttcc attgatagat agaggctgcc caagatagta
     3661 gccgtccttt tccgcctgtg atctgaaacc cccgggtctc gaaggaggtg gcaacacgcc
     3721 ctaaagcgtc cacccgcgta cccaagaagt ttcctggaag cgacaaaatc actgggagaa
     3781 agagaagaat gaccacaaca gcaccaagcg cgatcaggct taagggttta accgacaatt
     3841 gcttgccttc ggttttcttc tttggattct tctctttaac ctctgttcgg ggtgcggaag
     3901 gcaagaaaat gcgtttccga taccagaaga ccaaaactcc gaaaatgagg aatccgccca
     3961 tcaaggctaa atagacggcg cgggtgccgg ccaagagaac gaccaggtag aaaagaaaga
     4021 agcagacgac tccaaatgtt ttaatcccaa ggatgaagaa gaaacgtttt tgatcgaccg
     4081 aatccgattt gttgcgaaat ccgtactgat tgctgatcgc gaaatatatg gcgatcggga
     4141 tcaaagaggc cagaaagtcg gagacgaagt tcgggttgcc tatcgtcgcc ttcatttgca
     4201 tctttcccga cccgccaaaa cctccgagga aaaacgactg cccgtcgtag tagttcatca
     4261 aggcttccac ggcgacaaag aaaccggcga tcaacaccgc ggtcaagaag aacagaattt
     4321 cttttttctc ttcgaagaag ttggaaaggt agacggaaaa gaagacagtg agtagcgcgt
     4381 acatccccaa ctcaaaggga taggggaagt atagcgcgtt ctctcgcaac atcgcgaaag
     4441 aggcgacgac cgctgaagcc ccaaacaaga agtagcctaa atggggccaa gccaggtaca
     4501 agctcctgtc tttttccatc aactttctca cgatgaacag aaccatcaaa gcgaccgcga
     4561 agatcaccaa gaaaaagtat tttggtgtgc tgaattcata ggttagccgc gggtgcgcga
     4621 acatcgtaat gaaaatgaac gtaaaacaca agaataacat ctctatcttt ttcatttctc
     4681 caccccactc atcatatgaa accgtcatgt tcgccgaaag tattgtatca caacggcgag
     4741 gtcatttcat ttcgtatcct tgaggttttt ccggttgacg tttgcgcgtc agcgctaccg
     4801 ccacaaacag aaagaagagc gcgccgtatc cgcttaagca caacagacga tagtcaagga
     4861 aggtgatcgc ccgtgtttga tcggagaacc cgataagaaa cacgcccaag cctttttcga
     4921 agggggcgat ccgggcaacc acccgtccga aagggtctga gacccctgtg atcccgttgt
     4981 ttgagacctg tcccacccat ttccccgttt caagcgcccg aaaacgcgcc tgaacgaaat
     5041 gctgctccaa cgcgatcggg ctggtgaacc aggcgtcgtt ggtcaaaacg aagaaggctt
     5101 ccgccttctg cgctaaaaag tctccgaaga cttcggagta gtaagactcg aaacagacgg
     5161 gaaaggcgta gcgtttccca ctgatctcaa aggtctgggc aaacgggccc ggcgtgaaat
     5221 agttcgaaaa cttgatgaag ctgagcatcc cgaagatacg ctcgaagggt aaggtttcta
     5281 cgaaggggtt caaatgccgc ttgccgtaaa aatcgttgga aaccccgcct tccggtgaaa
     5341 cggtccgaac cgcgttataa tactcttcat cattcgtttg cgcgaggtgc cctaccacaa
     5401 aaaccacttc acgctccgtc gaaagccgct ccaacgcctt ggcggtcttg gtgcgctcgc
     5461 gaatatcata catgaattgc gcttccggaa acgcgacgaa atcgggtgtc tttggcaccg
     5521 agtccagcag gctcaataca tcggaaaact cttcgatgaa ctcagcttca gagaacgtgt
     5581 acttgtcctc ggagcgaaac atcgtctgca tcccgccgac ccaacgcgtc tctccgttct
     5641 gtgggtaaac cgggggtaaa aggttttgcg tgaccgaatc ggcggcgtag agtgagagca
     5701 ggagcaacgc caccaacacc caggaacgct tggaagacga tagaaaaggg cgcgccgata
     5761 ggattcgaaa gagcgcagca tttaccactc cgatcaagat caccagggct tcggtcccgc
     5821 cgaaggccac gagttgcgac aggcccagca tattcacgag agcatcggaa aaacgaaagc
     5881 cggggaaacc catcggcccg agcccgcgca accattcggt cacaaagagc aacagaccga
     5941 tgaaaaggca gaaaagcgta ggacgatcgg tgacccggct tctaaacagg gtgtaaacga
     6001 aggcgaaaag cccataaaag agggcttcca ccgatattaa cagtaggaag acaactgcgc
     6061 ccataatggg cgggaaggag cgcaacacct gggggatgta gtgggcgaag gtcggcagga
     6121 accaatgcaa gctgacgcca aagaatacga ggccgaagcc cagtcccctc aacgtgttgc
     6181 gtatcaaaga acgggcggaa agcggcgcgt tttctcggtc aagggaacgc aaaaacaacg
     6241 cgaacccgat ccagacgaaa aaatgaaggt tgaaacccgg catcgccaag gccatcagga
     6301 taccggctcc tatccatagg ccgcgttttt tcatgagaag cgttctattc cttcttttag
     6361 ggaatgaacc acgtactcct ggtcttcttc tgtcaggttg ttgtagaaag gaatcgccag
     6421 cgtccgttt
//
LOCUS       DAOPXN010000130         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12026_length_6416_cov_5.742022, whole genome shotgun sequence.
ACCESSION   DAOPXN010000130 DAOPXN010000000
VERSION     DAOPXN010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12026_length_6416_cov_5.742022"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="PKM49_07475"
     CDS             <1..855
                     /locus_tag="PKM49_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNY82688.1"
                     /translation="NSYTRAAKYLSLTQPAVTQHIKQLERELNIKIFNRVGREIKPTN
                     EGNIVIQYARRSIALYQRMEQSLLDEQRQIRRLTVGITHTAESNAVAEVLGKYCAENP
                     GTSITIITDSINHLYGMLKNYEVDLAVVEGRVQDASINTLLLDTDSLVFVVSNNSPLA
                     KKSMVTLNEIKAQPLILRRPSSGTRNLFIAHLESNNMSLNDFNVILEVDNIATIKDLI
                     RRDIGVSILAKSACLDELKKGKITVLPIENLSMIREINILYHDDFKHIDILQSILKEY
                     NKVVKLYA"
     gene            complement(944..1612)
                     /locus_tag="PKM49_07480"
     CDS             complement(944..1612)
                     /locus_tag="PKM49_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB domain-containing protein"
                     /protein_id="HNY82689.1"
                     /translation="MENVKRIFLEEHQMEIALIGTMHVSQDSASAVQRLILEEGPDTV
                     CIELDESRMDAIAHYRESGREGHGANRSLERLLESFQKQIGDKMNTAPGADFSVAIES
                     ARTVQARVVLIDRDIRTTLDRMWKALGPFGKLRFFFYLVREMRQARLMKQEELESLTE
                     QQEVFEALEELAERFPALKRTLVDERDAHMAARIKECGGKKVVVVLGAGHLLGVTRLL
                     QMAP"
     gene            2003..3367
                     /locus_tag="PKM49_07485"
     CDS             2003..3367
                     /locus_tag="PKM49_07485"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HNY82690.1"
                     /translation="MKTLFKNIEALGTFDRNQTEWTQAAILVEDDTIAAIGSDPALEH
                     VQADEVIDCSGLVIIPGFVNTHHHLYQSLFRNVKGVQNAKLFDWLTFLYERWKHIDAR
                     AITSAAKVGIYEMMLSGVTTTSDMFYLFPFNQNHLFDAEIQAARETGVRFHPCRGSMS
                     LSKKDGGLPPDCVVQTETEILSESIRIIRTYHDPTPKSMLRIALAPCSPFSVSERLMV
                     ETLELAIKEDVLVHTHLAETQDEERHCLERFGLTPVALMEKWGWLNPNAWFAHCVWVS
                     ESDIERFAEHQVGVSHCPTSNMRLGSGIAPIVEMLRKPQIRVSLAVDGSASNDTGNMI
                     QEARNALLLQRVTKGADALAVKTALECATYGGARVLRREGELGSLEVGKAADFIGFSV
                     DRLEFAGGLSDPIAALVLCDAKRVDLSVINGEIRIRDGRLLGVELSELIREQNRISKE
                     ILTR"
     gene            3412..5145
                     /locus_tag="PKM49_07490"
     CDS             3412..5145
                     /locus_tag="PKM49_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82691.1"
                     /translation="MHNLIRYMGTPRVFTEVLEYLYKNQGKGGLILTKTESGSWIVQD
                     SSGYIPQQYLYTSSEEILREIPDYLRYPIGSDTLLLSAIPIDESSLQTITKVVEIQQL
                     YRTTEKQGETISKLVYSITGIEAILNTILEPVEREEMIGILNDAMGELLLSFTVLYRR
                     GKNGYELLHAHGLSVPPSLLKFSSGNSYPLTFSFPVDLKKNRLFPEWTQLSDFRGLYG
                     IPIIMEGTIRYVIIAGKNEAYGESDRTILESLTRVISKIIEFYQLKEDFEMKERAVDR
                     YNFQFLSFFKGFQYLSAQDTLSDKIKILLDMYHELFQPSMLLPYYKKTWSQILWPYPE
                     NGIYPALYVKVVSCWDIYHLDKRTEREAFFDDFGNAESLLAVLTQEGHHPTLATILRS
                     SDGDVLALLLFERFPPQDYEFLNILNALTGLSLHDYFNRSEIKKLENTYENVMKTLQN
                     VNELYVEARKTVGLSGFYEMAERFFSNQFQTTGLYLLFSKDGKPILIPENTNPVISEK
                     LFQHYEDQSEELWMDYLPETGTILFTLPASHKGKHLFFGLESRDEAKISFLIQLLRLG
                     FKDLLAQIIFD"
     gene            5159..>6416
                     /locus_tag="PKM49_07495"
     CDS             5159..>6416
                     /locus_tag="PKM49_07495"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HNY82692.1"
                     /translation="MEKNPLTLIGTQLVRSDASEKVLGAAKYVDDLHFSDTVYLAIRT
                     AEVASGALRSIDISKARGSDGVLAVLTHADIPGENQMGSPVPDYPILLKPGDEIRYVG
                     DYVAFVVAHTREQALRAAEKVTVMVAEKKPVLSLNERIRELQKGGDYVPAVHQKIRKG
                     DSDPKTMFDSCDYVFEGDFFADYQEHAYLETQGMIALYDQDNVMTIYGSMQCPYYVQN
                     GVARALGFPINRIRIIQSVTGGAFGGKEEVPTYFAIPAALAAYHLHRPVKLVLSREED
                     IQWTSKRHPIKSRYKVGIADYGRLRSIQVEAHADIGGHSTLSPTVLWRSSVHAAGAYE
                     IPNVRVDVYGHYTNKVPSGAYRGFGSPQVFIGIEGIIDDVADHLKKDRLYLRKINALE
                     KGKTTPTGHLLPESVGAKKTLEEVERL"
BASE COUNT         1667 a         1725 c         1610 g         1414 t
ORIGIN      
        1 aacagttaca cgcgtgcggc caaatacctt tctttgactc agcccgccgt cacccagcac
       61 atcaaacaac tggaaaggga gctcaacata aaaatattca accgcgtggg aagggagata
      121 aagccaacga acgaggggaa cattgtcata caatacgccc gaaggagcat cgccctttac
      181 cagagaatgg agcagagtct tttagacgaa caacgccaaa tcaggcggct gaccgtcgga
      241 ataacccaca ccgccgaaag caatgcggtg gcggaggtgt tggggaagta ctgcgccgaa
      301 aacccaggta cgagtataac gataattact gactccatca atcatcttta tgggatgctg
      361 aaaaactacg aggtggatct ggccgtcgtg gagggaagag ttcaagacgc cagcattaat
      421 actcttttac tggacaccga ctcgctcgtg ttcgttgtat ccaacaacag ccccttggcc
      481 aaaaagagca tggttacgct caacgagata aaggcgcagc ccttgatcct acgcagaccg
      541 tcttccggga cgaggaacct tttcatcgcc cacttagaaa gcaacaatat gtcactcaac
      601 gattttaacg tgatcctgga agtcgataac atcgcaacga tcaaagacct catccgccgg
      661 gatatcgggg tttctatcct ggcgaaaagc gcatgcttgg atgaacttaa aaaagggaag
      721 attacggtcc tgcctataga aaacctcagt atgatccggg agatcaacat tttatatcac
      781 gacgacttca agcacatcga cattcttcag agcattctga aagaatataa caaggtcgtg
      841 aagttatacg cttaaacaag aggaagatag agccttgaaa ccagccatcg agcgggacag
      901 gaggagcgga cgggaccttc tctgaacggg tcggccggaa tccttaaggc gccatttgca
      961 gaagccgcgt cacgcctagg aggtggccgg cgcctagaac aaccaccact tttttcccgc
     1021 cgcactcctt gattcgggcg gccatatgcg cgtctcgctc atccacgagc gttcttttga
     1081 gcgcggggaa acgctcggcc aactcctcga gcgcttcgaa aacctcctgc tgctcagtca
     1141 gactttccaa ctcttcctgt ttcatcaatc gcgcctggcg catctcacga acgaggtaaa
     1201 agaaaaaccg caacttgccg aagggtccca acgctttcca catacggtct agcgtggttc
     1261 gtatatctcg atcgatcaag acgacccgag cctgaaccgt tctcgcgctc tctatcgcca
     1321 ccgaaaaatc ggcccctggg gcggtattca tcttgtctcc aatctgcttt tgaaagcttt
     1381 ccaacaagcg ctccaaagat cgattggctc cgtgaccctc tcgaccagat tctcgatagt
     1441 gcgcgatcgc atccattcgg ctttcatcga gctcgataca gacggtgtcc ggcccttctt
     1501 ccaaaatcag tcgttgcacc gccgacgcgc tgtcctggga tacgtgcatc gtcccaatca
     1561 acgcgatttc catttggtgt tcttctagaa agatacgttt gacgttttcc acgggcctcc
     1621 ttctatagac tcggaatagc cctcattata ccacgagggg ccaggcgcgt cacctcgcgt
     1681 gtgagtcaag ttatggagtg gcataactaa gaggttcatc gcggggtaag tggtttttac
     1741 gaccccgcga tccgccttat gccctctcaa aaacgtatcg cgccgtcgcg aaaataagaa
     1801 gcgcttacgc cgcgatgact atgtgggcta aaaatcgcgc gatcgattct ggccttgttt
     1861 cttgattaca ccccgtaact tgacccacac tcgggcttta ccgcgaaccc aaatatgcta
     1921 taattaagaa tggattcctc tccagtgacc gcaaaaatag gaaaagaagc cggcatcaca
     1981 tctttatatc aggagataga ggatgaaaac actgttcaaa aacatcgaag cgctcggaac
     2041 cttcgatcgg aaccaaacgg aatggaccca agccgctatc ctcgttgagg acgacaccat
     2101 cgcggccatc gggtcagacc ctgccctaga gcacgtacaa gcggatgagg tgatcgattg
     2161 ttccggcctc gtcatcatac ccggatttgt caatacccat catcacctat accagagcct
     2221 tttccggaat gtcaaaggcg tccagaacgc caagcttttc gattggctga cctttctata
     2281 cgaacgatgg aaacacatcg acgctcgagc gatcacctcc gcagcgaaag tcggcatcta
     2341 cgaaatgatg ctctccggag tgacgacgac atcggatatg ttctatcttt tccccttcaa
     2401 tcaaaaccat ctgtttgatg cagagataca ggcagcgcgg gagaccggcg ttcgttttca
     2461 tccttgccgg ggcagtatgt cgctgagcaa gaaagacggc ggattgccgc cggactgtgt
     2521 ggtgcagaca gaaacggaga tcctgtccga atcgatccgc attatccgaa cctaccacga
     2581 tccgacaccg aaatcgatgc tgcgtattgc cttagcgcct tgcagcccgt tttctgtttc
     2641 cgaacgtttg atggtggaaa cgcttgaact ggcgataaaa gaggatgtgc tcgtccacac
     2701 gcacttagcg gaaacgcaag acgaggaacg gcattgtcta gaacggttcg gtctgacccc
     2761 ggtcgctttg atggaaaaat ggggatggtt gaatcccaac gcctggttcg cgcactgcgt
     2821 atgggtgagc gagtccgata tcgaaagatt cgccgaacat caagtcggag tctcccactg
     2881 tccaacctca aatatgcgat taggctccgg aatcgcgccg atcgtagaga tgctacggaa
     2941 gccgcaaatc cgtgtttcgc tcgcggtgga tggcagcgcc agcaacgata cgggaaatat
     3001 gattcaggag gcgcgaaacg cgctgttgtt gcaacgcgtg acgaaaggcg cagacgccct
     3061 agcggtaaaa accgcgctcg aatgcgccac ttatggtgga gcccgtgtct taaggcgaga
     3121 aggtgagttg ggctctctgg aggttggaaa ggcggcggac ttcatcgggt tttccgtcga
     3181 ccgcctcgag ttcgcgggag ggttgtccga cccgatcgcc gccctcgtcc tctgtgacgc
     3241 gaagcgcgta gacctttcgg tgatcaacgg ggagattcgg atacgggatg gccggttgct
     3301 cggcgtcgaa ttaagcgagc tgatccgaga acaaaatcgt atctccaaag agatccttac
     3361 ccgataaggc ataagagccg acaagcggcc gaacctagga aaggtgatgg aatgcataat
     3421 ctgatacggt acatgggtac accgagggtt ttcacagaag ttctggaata cctatacaaa
     3481 aatcaaggca aaggcggatt aattctgacg aaaacggaaa gcggcagttg gatcgttcag
     3541 gactcctcgg gatatatccc tcagcaatac ctgtacacct catccgaaga gatactccga
     3601 gaaatccccg attaccttcg ttatccgatc ggctcggaca ccctgctctt gtccgcgatt
     3661 ccgatcgatg aaagcagcct tcaaaccatc acgaaagtgg tagagataca acaactctat
     3721 cggaccacgg aaaaacaggg agaaacgatc tccaagctcg tctatagcat caccggcatc
     3781 gaagccatcc tcaatacaat cctggaaccg gtcgaaaggg aagagatgat cggcatcctt
     3841 aatgacgcga tgggtgagct tttactgagc ttcacggttc tttaccgacg aggaaaaaac
     3901 gggtacgagc tgcttcacgc gcacgggctg tccgtccccc cttcattact gaagttctct
     3961 agtgggaact cctacccgtt gaccttttct ttccccgtcg acctgaaaaa gaaccgattg
     4021 ttcccagaat ggacccagtt gagcgacttt cgcgggctct acggtatccc gatcatcatg
     4081 gaggggacca ttcgatacgt catcatcgcc gggaaaaatg aagcttatgg agaaagcgac
     4141 cgcacgattc tagaaagttt gacccgcgtc atctcgaaga tcatcgaatt ctatcagctc
     4201 aaggaagact tcgagatgaa ggagagggcg gtggaccggt acaacttcca gttcctctct
     4261 ttcttcaaag ggtttcaata cttatcggcg caagataccc tcagcgataa gatcaagatc
     4321 ctgctagata tgtaccacga gctctttcag ccctctatgc tcctgcccta ctacaaaaaa
     4381 acctggagcc agatcctttg gccctacccc gaaaacggta tatatccggc gctgtatgtc
     4441 aaggtcgttt cttgttggga catctatcac ctcgataaaa gaacggaacg cgaagccttc
     4501 ttcgatgatt tcggaaatgc cgaaagcctc ctcgccgtat tgacgcaaga gggccatcat
     4561 cccactttgg cgacgatttt gagatcctcc gatggcgatg tgcttgcctt gcttcttttc
     4621 gagcgctttc ccccccagga ctatgagttt ttaaatattc taaacgcgct tacggggttg
     4681 agccttcacg attacttcaa tcgatcggag atcaaaaagc tcgagaacac ctatgaaaac
     4741 gtgatgaaaa ctttgcaaaa cgtcaatgaa ttgtacgttg aggccagaaa aaccgtcggc
     4801 ctctcgggtt tctacgaaat ggcggagcgg tttttttcga accagtttca aaccaccggc
     4861 ctatacctgc ttttctctaa ggatggcaag ccgatcctca tccccgaaaa caccaacccc
     4921 gtgatttcgg aaaaactgtt ccaacactac gaagaccagt ctgaagaact ctggatggac
     4981 tacctacccg aaacgggcac gatcctcttt accctaccgg cttcacacaa gggtaaacat
     5041 ctgtttttcg gtttggaaag ccgcgacgaa gccaagatca gttttttgat ccaactattg
     5101 agattggggt tcaaagatct attggcgcag attatctttg attgatggga gaatgacgat
     5161 ggaaaagaat ccactcacgt tgatcggaac tcaattggtg cgttcggacg cgtccgagaa
     5221 ggtcttaggc gcggccaaat acgtagacga cttacacttc tcggatacgg tctatttggc
     5281 gatcagaaca gctgaagtcg cctccggagc gttaagatcg atcgatatta gtaaagcccg
     5341 agggtcagat ggcgtactgg ccgttttaac tcacgccgat atccccggcg aaaatcaaat
     5401 gggatcaccc gtacccgatt acccaatcct actcaagccc ggagatgaga tacgatacgt
     5461 gggcgattac gtcgccttcg tggtggcgca cacccgggag caggccttgc gcgccgcaga
     5521 aaaagtcacg gttatggtcg ctgaaaagaa gccggttttg tctttgaatg agcgtatacg
     5581 agaattgcag aaaggcggag attacgttcc cgccgttcac cagaagatac gcaaaggcga
     5641 ttcggatccg aagacgatgt tcgattcctg cgactacgtc ttcgaagggg actttttcgc
     5701 ggactatcag gagcatgcct acttggagac gcaaggtatg atcgccctat acgatcaaga
     5761 caatgtgatg accatctacg ggtcgatgca atgcccctat tacgttcaga acggggtagc
     5821 ccgagccctc ggctttccca tcaaccggat acgcatcatc cagtcggtca ccggaggggc
     5881 gtttggcggg aaagaagagg tgcccaccta tttcgccata cccgcagctt tagccgccta
     5941 tcacctgcat cggccggtca aattggtttt aagcagagaa gaagatatcc aatggaccag
     6001 taaacggcat ccgatcaaga gccgctacaa agtgggcatc gccgactacg gccgtttgcg
     6061 ctccatacag gtggaagcgc acgccgatat cggcggccat tccaccctct ctcccaccgt
     6121 tttgtggcga tcgagcgtcc acgcggcagg ggcatacgag atcccgaacg tacgggtgga
     6181 tgtctacggc cactacacga acaaagtccc ttccggcgcc tatcgcggtt tcgggtctcc
     6241 acaggttttc atcggtatcg aagggatcat cgacgatgta gccgatcatc tgaaaaaaga
     6301 ccgcctgtac ctgcggaaaa tcaacgcgct ggaaaaaggt aagaccaccc cgaccggcca
     6361 tcttcttccc gaatccgtag gagcgaaaaa aaccttggaa gaggtggaac gcctct
//
LOCUS       DAOPXN010000131         6351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12187_length_6351_cov_6.702351, whole genome shotgun sequence.
ACCESSION   DAOPXN010000131 DAOPXN010000000
VERSION     DAOPXN010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12187_length_6351_cov_6.702351"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            516..2006
                     /locus_tag="PKM49_07500"
     CDS             516..2006
                     /locus_tag="PKM49_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY82693.1"
                     /translation="MQAEAAIDRFLTIVSTFSRELNTAKKALDVYHAIVKVLRRFFDI
                     HSFCVLEQKGPSDWISIWSDPTFPFGFDELTPYVQLAQERRSFSIYPLDEGHLLILPS
                     LKSGRVVSVLMGYLQDDPEESLRENESVVGFIMFFSGLVLENLRLYREVLDSAAVQEG
                     LKKYYQTMLDSMDEAISVWDENRSLVFTNQSYIRADPSESILEIIKDLVTQSYSNRNH
                     NTLEKEIAGHFYSFGTVLLGNPDQVLVSMEDISNTKELERIKKLDQIRTEFVSNISHE
                     FRTPLAAIKAYAETIRDSFGSLDEETGQQFMMTILEQSEHLEGLLNRLLDFSRLENQA
                     MRPEIEDFDLIEQVRDVEQRVQKMLQTYAVSFSIETKLPEAVVRGDPKQINQVVFNLV
                     ANAIKYRDTKKLERWAKVIVEESEEEGKVTLTVEDNGIGIPEESADKVFEKFYRVDTS
                     LTYSVEGTGLGLAVVKEILDMHRAVITVKSQIGIGSSFSFSLPKAG"
     gene            2013..2858
                     /locus_tag="PKM49_07505"
     CDS             2013..2858
                     /locus_tag="PKM49_07505"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNY82694.1"
                     /translation="MELKKQVQLIESLPSPDFILKKIVDTASSSSASAKELNEVVSKD
                     TGFVSKLLKLANSSYYGLPKKVSKLTEAIMILGFKTVRNMALSIFANEQLFSFKSKEL
                     QLRELWIHSLSVATIGETIAERIGFSNKEELFLCGLLHDVGKTVQGILFPTLFDAIVK
                     VSRTKGWTYYRTEKELEVPTHEQLAEMLMEKWNFPELVTLAATRHHRTDDFGSSIYTD
                     VVYITSLSTYIAERMRYGDNGAGAPIPIKHNLWKELGLSPLILKDIARMSAEKINRLS
                     EFYST"
     gene            2935..3957
                     /locus_tag="PKM49_07510"
     CDS             2935..3957
                     /locus_tag="PKM49_07510"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNY82695.1"
                     /translation="MRVGVLTGGGDCPGLNAVVRGIVSARDPDTEIFGFMNGWQGTLD
                     GEYVHLTDEDVEGIHILGGTILGTSRTNPFKKEADTRKMFDTLAKLKIDALIAIGGDD
                     TLSVAAKMTAYHFPVVGVPKTIDNDVSQTDYTFGFHTAVNVGADAIDRLHSTAKSHKR
                     VFYVELMGREAGWIALEAGMAAGAHLIVIPEYPMTMDEILRMVDSRMKRGGYAIVAVA
                     EGFKPVDLDNTIQDDSQIDAFGHIQLGGIANYLKKITKEKLGYDTRSVILGHLLRGGT
                     PTAFDRILGSRYGVQAMELIKKKDFGRMVALKGNEIVSVPIEYAVATKKLVPYEYYKM
                     CSLFFG"
     gene            3978..4499
                     /gene="amrA"
                     /locus_tag="PKM49_07515"
     CDS             3978..4499
                     /gene="amrA"
                     /locus_tag="PKM49_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNY82696.1"
                     /translation="MEDKGEIVRWVKAVIDAYVKNRKVIPVPEDLSEALTGRRAGAFV
                     SIHTVDHRLRGCIGTIEPTQSSLALELRSNAIAAATRDPRFPPIEPEELDHLTISADV
                     LEAPERVKDMSELDPKEYGVIVKSGFRKGLLLPDLEGVETVEAQVAIAKRKAGIEEDE
                     PVTVYRFRVTRYH"
     gene            4520..5509
                     /gene="amrS"
                     /locus_tag="PKM49_07520"
     CDS             4520..5509
                     /gene="amrS"
                     /locus_tag="PKM49_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNY82697.1"
                     /translation="MREALFYEEIENGSVRCLLCPRHCVIDKGQVGFCRTRKNVDGVL
                     YTLIYGRLSSIATDPIEKKPLYHYHPGTKILSVGTWGCNMSCPWCQNHEISQGTPQTQ
                     KVDPERLVHIALDRNSPGIAYTYNEPMIWFEFVLDTSRMASADGLYNVLVTNGYVSAD
                     PFKLLLQTVQAMNIDLKGFDQRFYQKRCSADLEVVKRNIQLAYEEGVHVELTTLLIPD
                     ENDSVEKINEQCQWIASIDPAIPLHLSLYFPAYRFNKPPTDLNRAIELWKTAKKTLQY
                     VYLGNQLESEYQRTLCPYCSNVLIERSGYQIQIVGLGEGRRCQQCHRQIPIVL"
     gene            5470..>6351
                     /locus_tag="PKM49_07525"
     CDS             5470..>6351
                     /locus_tag="PKM49_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNY82698.1"
                     /translation="MSAMPSPDPHSLVTLQKRVWFYALLLLIFAVAVFSLLLTKKTAP
                     YTTKDAIQMGTYMRICLGTEKNPHTLIDAMIREVKRIENKYSFHNKENVLAEINWNGE
                     EWTYVDEETQFILERSYELALTTKGAFDPVLGRLTRLWGFDRIDKDDVAFRVPTDEEL
                     ATARQASGYRNLEIRGDTVRLKNGALLDLGGIVKGYAIDRCVQVARELDPSATGYVDI
                     GGDIGIIGPKFGGADWIISVQPPREIAGVRQMAAVFLKTGGIATSGDYQRYFEVDGKR
                     YHHILNGETGMPSTGTIS"
BASE COUNT         1667 a         1529 c         1779 g         1376 t
ORIGIN      
        1 tgcagagttc tcccgaaaag gaaaagacat gccggactaa agtccggcgt tcccaagcgt
       61 tcccagggcg tatcacaaga gtaaaaggcg gatcgcgggg tcgtaaaaac cacttacccc
      121 gcgataacac acacggtcat cgcggcgtaa gcgcctcttc ttctcgcgac ggcgcgatac
      181 gcttttgtac atgtctcgaa aaacagggtt cgcacagagg gctcagagaa cacagagaaa
      241 agactgaaga aaactcgcgg ggccaacttt taagttggca tgtcttgaaa aaagctcaca
      301 cagagggaac ggagaacaca gaggaaaaac tggcaaggtg cagagttctc ccgaaaagga
      361 aaagacatgc cggactaaag tccggcgttc ccagggcgtt cccattttgg tgtacctcga
      421 tattatgctc acacctcctc agagggtgag cgtcttttat gctataattg agtcactgaa
      481 cataaaatgg cccgaacgga taacgaggga tcattgtgca agctgaagca gccatagatc
      541 gttttctaac catcgtgtcc accttttcga gggaactcaa tacggcgaaa aaggccttgg
      601 atgtttacca cgcgattgtc aaagtcctga gacgattctt cgacatccac tctttttgcg
      661 ttctcgagca aaaggggcct tcggattgga tttccatctg gagcgatccg acctttccgt
      721 tcgggttcga tgaactgacg ccgtacgtgc agctcgcgca agaaaggcgg tcgttcagca
      781 tatatccatt ggatgaggga caccttctga tcttgccctc tctaaagtcc ggtcgggtcg
      841 tgtcggtact gatgggttac ctacaggacg atccggagga gtctttacgg gaaaatgaga
      901 gcgtggtggg gttcatcatg ttcttttcag ggctggttct cgaaaactta cgactgtatc
      961 gggaggtctt ggacagcgcg gccgttcaag agggcttgaa aaagtactat cagacgatgt
     1021 tggacagtat ggacgaggcc atcagtgtct gggacgaaaa tcgatcgtta gttttcacga
     1081 atcaatccta catccgggcc gatccgagcg agagtatcct cgagattatc aaagacctcg
     1141 tgacgcaaag ctatagcaat cgcaatcaca acactctgga gaaagagatc gcggggcatt
     1201 tctattcgtt cgggacggtt cttttaggga atcccgacca agttttagtc agtatggagg
     1261 atatctccaa caccaaagag ttggagcgca tcaaaaaact cgatcaaatt cggaccgagt
     1321 tcgtttcgaa catctcgcac gagttccgaa cgccgctcgc agcgataaag gcttacgcgg
     1381 agaccatacg cgactctttt ggctctctcg acgaggagac cggtcagcag ttcatgatga
     1441 ccatattaga gcagagtgag catttggagg gacttcttaa tcgactgctg gacttctcac
     1501 ggctggagaa ccaagcgatg cgccccgaga tcgaggactt cgatttgatc gagcaggttc
     1561 gagatgtgga gcagcgcgtc cagaagatgt tacagacgta cgccgtgtct ttttcgatcg
     1621 aaaccaagct ccctgaagcc gtcgttcgtg gcgatccgaa gcagatcaac caggtagttt
     1681 tcaatttagt ggcaaacgca atcaaatatc gtgacacgaa gaagctcgaa aggtgggcga
     1741 aggtgatcgt cgaggagtcg gaggaagagg ggaaggtgac gctgacagtc gaagataacg
     1801 gcataggtat tccggaagag agcgcggata aggtcttcga aaaattctat cgggtggata
     1861 ccagcttaac ttactcggtg gaaggcaccg ggttgggttt ggcggtcgtg aaagaaatac
     1921 tcgatatgca ccgcgcggtg atcacggtca aaagccaaat aggaataggg tcttctttct
     1981 cgttttcctt gccgaaggct gggtaaccgg ctatggaact aaaaaaacag gttcaactga
     2041 tcgaatcgct gccttctccg gatttcatac tcaaaaagat cgtcgatacc gccagtagct
     2101 cctccgcttc ggctaaggag ctcaacgagg tcgtatccaa agacacgggg ttcgtcagca
     2161 aactgctgaa gctggcgaac tcgtcttact acggattgcc gaagaaggtg tcgaagctga
     2221 ctgaggccat tatgatcttg gggtttaaaa ccgttcgcaa tatggcctta agcatcttcg
     2281 cgaatgaaca gctgttttcc ttcaaatcga aggaactgca attaagagaa ttgtggattc
     2341 attcgctatc ggtcgccacg atcggagaaa ccatcgcgga gcgtatcggg ttttccaata
     2401 aagaggagct gtttctgtgc gggttgctgc acgatgtcgg aaaaaccgtt caaggaattc
     2461 tctttccgac gcttttcgat gcgatcgtga aggtgtcgcg cacgaaagga tggacctact
     2521 atcgtactga aaaggagctg gaagttccaa ctcacgaaca actcgccgag atgctgatgg
     2581 agaaatggaa cttccccgag ctagtgacct tggccgcgac caggcatcat cggacggacg
     2641 actttggcag ttccatctat accgatgtgg tgtatatcac gtcgctttcc acctatatcg
     2701 cggagcggat gagatatggc gacaacgggg cgggcgcgcc gatcccgatc aagcacaatc
     2761 tttggaaaga gctgggcctt tccccgttga tcctcaaaga catcgcgcgg atgagcgcag
     2821 aaaagatcaa tcgattgtca gagttttaca gtacataatc gaaaaagaac cgtaaggagc
     2881 gctcggtcta tcaaaagggt atcaccgggc aaaaaactaa aggaggaaag agagatgcga
     2941 gtgggcgtgt taacgggcgg tggggattgt ccaggactaa acgcggttgt gagaggtatc
     3001 gtctccgcca gggatccgga taccgaaata ttcggtttta tgaacggctg gcagggcacg
     3061 ctggatggag agtacgtcca tttgaccgac gaggacgtag aggggatcca tattttgggt
     3121 gggacaatct tgggaacatc acgaacaaat ccgtttaaaa aagaggcgga tacccgaaaa
     3181 atgttcgaca ccttagcaaa actgaagata gacgcgttga tcgcgatagg gggcgacgat
     3241 accctctcgg tcgccgcgaa gatgacggcc taccacttcc ccgtcgtagg cgttccgaaa
     3301 acaatcgaca acgacgtttc acaaacggat tacacctttg ggtttcacac cgctgtcaac
     3361 gtcggggccg atgcgatcga ccgcctacat tcgacggcca aatcgcacaa acgggtcttc
     3421 tacgtggaac tgatgggtcg cgaagccggt tggatcgcgc tggaggcagg gatggccgcg
     3481 ggagcgcatc tgatcgtgat tcctgaatat ccgatgacta tggatgagat actgaggatg
     3541 gtcgacagcc ggatgaaacg aggaggatac gcgatcgttg ccgtcgcgga aggcttcaag
     3601 ccggtagatc tcgacaacac catacaagat gactctcaga tcgatgcctt cggccatatc
     3661 cagctgggcg gcattgcaaa ttacctgaag aagatcacaa aagagaagct gggatacgat
     3721 acccgatcgg tcatcttggg acacttgctt cgcggtggca cccctaccgc tttcgaccgt
     3781 atactaggct cacgctacgg ggtgcaggcg atggaattga tcaagaaaaa agacttcggt
     3841 cggatggtcg ctttgaaagg gaacgagatc gtttcggttc cgatagaata cgccgtcgcg
     3901 acgaaaaaat tggtacctta cgaatactac aagatgtgta gtctgttttt cggatgagcc
     3961 ttcgaggagg cgagcgtatg gaggacaagg gagagatcgt tcgatgggta aaggccgtca
     4021 tagacgctta cgtcaaaaat cgaaaagtca ttccggttcc cgaagatcta tcagaagcat
     4081 tgaccggcag aagggccggg gccttcgttt caatacacac cgtcgaccac cgtttgaggg
     4141 gatgtatcgg cacgattgag ccaacccaga gctcgttggc cttagaactc cgctcaaatg
     4201 ctatagcggc cgcgacgcga gaccctcgat tccctcctat agaaccggaa gagctcgacc
     4261 atttgacgat ttcggcggat gtgttggagg caccagaaag agtgaaggat atgagcgaat
     4321 tggatccgaa agagtatggc gtaatcgtga agagcggctt ccgaaaaggt cttctacttc
     4381 cggatctcga aggcgtagag accgtggagg cgcaggtggc gatcgctaaa aggaaggccg
     4441 gaatagagga agacgagccc gtcacagtct atcgttttcg cgtcacgaga taccattgag
     4501 cgctaaagag gtgtttgaag tgcgtgaagc cttgttctac gaggaaatag aaaacgggtc
     4561 ggtacggtgt ttgctgtgtc cgaggcattg tgtcatcgat aagggacagg tggggttttg
     4621 ccggacgcga aagaacgtgg acggcgtttt gtataccttg atctacggac gactctcctc
     4681 gatcgctacg gatccgatcg aaaaaaaacc gctctatcat tatcatcccg ggacgaagat
     4741 cctttctgtc ggaacgtggg gatgcaacat gagctgccct tggtgccaaa atcatgaaat
     4801 ctcacaaggc acgccgcaaa cacaaaaagt ggacccggaa cgactggtgc acatcgcttt
     4861 ggaccggaat tctccgggca tcgcgtacac ctacaacgaa ccgatgattt ggttcgaatt
     4921 cgtgctggac acctctcgaa tggccagcgc cgacggcctc tacaacgtct tggtcacgaa
     4981 cgggtacgta tcggccgacc cgtttaagct ccttttacag acggtgcagg cgatgaatat
     5041 cgatctgaaa ggttttgatc aacggtttta ccaaaaacgc tgctcggcgg atctcgaggt
     5101 ggtcaaacgc aatatccaac tcgcctatga agaaggcgtg cacgtcgaac tcaccacgct
     5161 actcatcccg gacgagaacg acagcgtcga aaagattaac gagcagtgtc agtggatcgc
     5221 ctcgatcgac cccgcgatcc ctttgcacct gtcgctctac tttcctgcct accgattcaa
     5281 caagccgcca acggacctga accgcgcgat cgagctttgg aaaacggcga aaaagacgct
     5341 tcaatacgtc tatctcggaa accagctgga atccgagtat cagagaacgc tctgcccgta
     5401 ctgctcaaac gttcttatcg aaaggagcgg gtatcagata cagatcgtcg ggctggggga
     5461 agggagaaga tgtcagcaat gccatcgcca gatccccata gtcttgtaac cttacaaaaa
     5521 agggtctggt tttatgccct gttgctcctc attttcgctg tcgccgtctt ttccttactt
     5581 ttaaccaaga aaaccgcgcc ttacacgacg aaagacgcga tccagatggg gacctatatg
     5641 cggatttgtc tgggtaccga gaaaaatccg cataccctga tcgacgcgat gattcgggaa
     5701 gtgaaaagga tagagaataa gtattctttc cacaataagg aaaacgtgtt agccgaaatc
     5761 aattggaacg gtgaagaatg gacctatgtg gatgaggaaa cgcagttcat cttggaaagg
     5821 tcctacgaat tggcgcttac cacgaaaggc gccttcgatc ctgttctcgg gcgtttaacg
     5881 aggttgtggg gcttcgaccg gatagacaaa gacgatgtcg cctttcgcgt gcctaccgac
     5941 gaagagctcg cgaccgcccg ccaggcatcg ggatatcgca acctggagat ccgaggagac
     6001 accgtgcgcc tgaaaaacgg cgcgctcctg gacctggggg gaatcgtgaa gggatacgcg
     6061 atcgaccggt gcgtacaagt ggccagggaa ttggatccga gcgccaccgg ttatgtggac
     6121 atcggcggtg atatcggcat tatcgggccg aagttcggag gcgccgactg gattatcagt
     6181 gtgcaacccc cccgcgagat cgcgggagtg cgacaaatgg cggcagtttt tttgaaaaca
     6241 ggcgggatcg cgacctccgg cgattatcag cgctattttg aggttgatgg gaagagatac
     6301 caccatatcc tcaacgggga gaccggtatg ccttccaccg gaacgatctc g
//
LOCUS       DAOPXN010000132         6253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12440_length_6253_cov_5.804130, whole genome shotgun sequence.
ACCESSION   DAOPXN010000132 DAOPXN010000000
VERSION     DAOPXN010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12440_length_6253_cov_5.804130"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1211)
                     /gene="dnaJ"
                     /locus_tag="PKM49_07530"
     CDS             complement(78..1211)
                     /gene="dnaJ"
                     /locus_tag="PKM49_07530"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNY82699.1"
                     /translation="MAKKDYYEVLGVTKEATQDDVKKAYRRLAKKWHPDLHKDDRAQA
                     EKMFKELTEAYEVLSDAEKRAMYDRYGFVGDQTPPTYRTATTGRESGSVFNDFFGGQP
                     MGDIFDMFFGSQGHATRVRPEDASVRMGEDLQITVQVALKDVMRGFDEEIEYERYTAC
                     ESCKGNGSKDGNSFRTCSQCGGRGAIIQETRTLIGNISSSRTCPKCRGTGKVIEESCP
                     VCKGNGRLAERKKIRIKIPAGAEDQMRLRIAGAGNAGIGGGGAGDLYVMIRVQPHRNI
                     KRQGKDLYSEVGVSYLQAILGGTIEVETLEGSVSYKLDAGTQPNETIRLRGKGLPDLR
                     SGLLGDHYVTVKVTIPKRLNKKERKLLEEIAALKNEPVEQEVG"
     gene            complement(1263..1832)
                     /locus_tag="PKM49_07535"
     CDS             complement(1263..1832)
                     /locus_tag="PKM49_07535"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNY82700.1"
                     /translation="MKTAEEKMDSLKKKLKSKEAEIERLKEIERYAKQLKSDFEHYRQ
                     LSQKEQQRTVNNANERLIEKMIPILLNFERATKHTPVQKATPEVQRVFKGYEMIYQMM
                     RSVLENEGLQPIDALIGLPFDPFEQEVVEKVETDTAPEGSVLEVVEGGFKFRGKVLIP
                     VKVKAAVTPQTVDSNDGSNLKTAPNPEKA"
     gene            complement(1836..2882)
                     /gene="hrcA"
                     /locus_tag="PKM49_07540"
     CDS             complement(1836..2882)
                     /gene="hrcA"
                     /locus_tag="PKM49_07540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNY82701.1"
                     /translation="MELTGRQSTILYAVIHEYVTSRVPVSSKRILEITNLECSSATIR
                     SEMKTLEGLGFIEQPHTSAGRVPTDKGLRFYVECLKCVRQETAQTSAELEMVSEYALT
                     NVEDYLQNVTVFLSHWMNGMAVIERPFIDRLRIQRITISEIAKNYWMVILVTDMGLAE
                     NFLLFLEDTLEATSLEQYLTQKLHGLPLEEVKPLLDTIHFQDHTWYDFHYEPLFLFLG
                     KMVQQATQVKYHKHGMEKLIQDDSIPKESLKKFIGFMQNERQLADLFDELQKDGHTTA
                     ITIGREMRREELLDFSLFFTGYRLEDKPIGRIVVFTSKVTDYWDNLKSLQYAGDRLTE
                     YLTKITLMGRSREV"
     gene            3960..5957
                     /locus_tag="PKM49_07545"
     CDS             3960..5957
                     /locus_tag="PKM49_07545"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82702.1"
                     /translation="MKLKSMRAKLLVFFIPVSVVVLVTVGLIVALLARNVTYNQAVDL
                     SAETMTAASRIIEEWLMGVKNEMRNLANTDVVRSFYPDFFIATFLEAIQQSGGVYQSI
                     LLADTKGKAISERGIEVDISDRNYFKEIVLQGKEFTVSEGLHSRFTNDYAVQVAHVVF
                     DRHGNRIGLLGASISLKTLSEKAATITLGKGGYTFLTDGSGRVIAHNRSDLIMKLNVL
                     EAEKSGYKGLEEAGREMVRGHEGIHNIVQPTGEKQILLYYPVPNTPNWSLAGVISEAE
                     VEEKSTFLLGIVVLCFAIIVAIIVVISMFVGTIISKSLNKLAVQVDQFGEGDLTTTFE
                     VKGHDELARIARALSGMGHDLREAMVSIEGTSKDIKTASTDLAAISEEQLAGSEELSS
                     QSHSVNTNLQNTVAAIEEVDSGVEEVAASAQNVSRTAQALSIENDKTADGSRRGGQMV
                     SDVVREIEATTQQTLLTAEKVQQLSEHSKNVGEIVDTISSIAEQTNLLALNAAIEAAR
                     AGEAGRGFAVVADEIRKLAEESKKATSNIGTILKEVQKNAEESYQATDKTVVLVKGVN
                     EKSQAVEQQFKQLLSMVEKTTVMIENLSTTSEEQGAAAEEVASAMNTSAKSAQEISER
                     IGQMTRGIEQQAEGARQLSAAAEELDTLAENLDTQIKRFKI"
BASE COUNT         1428 a         1604 c         1605 g         1616 t
ORIGIN      
        1 gcagcgcttt taagaacctg ccggactaaa gtccggcgct cccagtcgtt cggagttcca
       61 agaaaggcgc tccgggacta gccgacctct tgttctaccg gttcgttctt cagcgctgcg
      121 atctcttcga ggagtttacg ttcttttttg ttcagtcgtt tcggtatggt cacctttacc
      181 gtgacgtaat ggtcccctaa aaggccgctt ctcagatcgg gcaggccttt tccgcgcagc
      241 cggatcgttt cgttcggttg ggttccggcg tctagtttat aggatacgct cccttccaac
      301 gtttctacct cgatcgttcc gcccaaaatg gcttggagat agctaacgcc gacctccgaa
      361 tagagatctt tcccttgacg cttgatattc cggtgcggtt gaacccggat catcacgtat
      421 aggtctccgg cgccgcctcc cccgattccg gcgttacccg cgccagcgat gcgaaggcgc
      481 atttggtctt ccgctccggc ggggatcttg atacgtatct tttttcgttc tgccaaccga
      541 ccgttacctt tgcaaaccgg gcagctctct tcgatcacct ttccggtgcc ccgacatttc
      601 ggacaagtgc gggaagagga aatgttcccg atcagggttc gggtttcttg tatgatcgcg
      661 ccgcgcccac cgcactgcga gcatgttcgg aatgagttcc cgtctttcga gccgtttcct
      721 ttgcaggatt cgcacgcggt ataccgttcg tattctatct cttcatcgaa gcctctcatc
      781 acgtctttta aggccacctg tacggtaatc tgtagatctt ctcccatcct gacgctggcg
      841 tcttccggtc tcacccgcgt cgcgtggcct tggctcccga aaaacatatc gaagatatct
      901 cccatcggct gacccccgaa aaagtcgttg aataccgatc cgctttctct tcctgttgtc
      961 gccgttcggt aggtcggtgg cgtttgatcg ccgacgaagc cgtaccggtc gtacatcgct
     1021 cgcttttccg cgtcagacaa cacctcgtag gcttcggtga gttctttgaa catcttttcg
     1081 gcttgggcgc ggtcatcttt gtgcaaatcg ggatgccact tcttcgccaa tctcctatag
     1141 gctttcttca catcatcctg tgtggcttct ttggttacgc ccaacacttc ataatagtct
     1201 ttcttcgcca tctcttcacc ttcttattcc ggacagactc tccggaaaac ctttcccgtc
     1261 gcttatgctt tttcggggtt tggagcagtt ttgaggttag atccgtcgtt ggagtcgacg
     1321 gtttgcgggg ttaccgcggc cttgactttc accgggatca ggacctttcc cctgaacttg
     1381 aaccccccct cgacgacctc taagacggag ccttcgggcg cggtgtccgt ttccactttt
     1441 tctacgactt cctgctcaaa ggggtcgaac gggagtccga tcagcgcgtc gatcggttgc
     1501 aacccctcgt tttcaaggac cgagcgcatc atttggtaga tcatctcgta tcctttgaaa
     1561 acccgctgga cttcaggcgt cgctttttga actggcgtgt gcttggtggc gcgttcaaaa
     1621 ttcagcaaga tcgggatcat tttttctatt agcctttcgt tcgcgttgtt caccgtccgc
     1681 tgctgctctt tttgcgacaa ttgccgatag tgctcaaaat cggatttcaa ttgtttggca
     1741 taccgctcta tctcttttaa acgctctatc tcggcctctt tagacttcaa tttcttcttc
     1801 aatgaatcca tcttttcctc ggctgttttc aaacgtcaca cctcccgcga acgccccatg
     1861 agtgtgatct tcgtgagata ctccgtcaaa cggtcgcccg catattgtaa actctttagg
     1921 ttgtcccagt aatcggtcac tttggaggta aacacgacga tgcgtccgat cggtttgtcc
     1981 tccaatcggt aaccggtgaa aaacaaagaa aaatcgagca gctcctcgcg gcgcatttca
     2041 cgtcctatcg tgatcgcggt ggtgtgccca tccttttgta gctcatcgaa caagtcggcc
     2101 agttggcgct cattttgcat aaacccgatg aattttttca ggctctcctt cggaatcgaa
     2161 tcgtcttgga tgagtttttc catcccatgt ttgtggtact tcacctgggt ggcctgttga
     2221 accatctttc ccaaaaagag aaagagcggt tcatagtgga aatcatacca ggtgtggtcc
     2281 tggaaatgta tcgtatcaag caacggtttg acctcctcaa gagggagtcc gtgtaacttt
     2341 tgagtgaggt attgttccaa cgacgtcgct tccagagtgt cctccaaaaa caacagaaaa
     2401 ttctccgcca agcccatatc ggtcaccaaa ataaccatcc agtaattctt cgctatttcg
     2461 gatatggtga tccgttgtat ccgtagccga tcgatgaaag gcctttcgat caccgccatc
     2521 ccgttcatcc aatgggacaa aaagaccgtc acgttctgta aataatcctc gacgttggtc
     2581 aacgcgtact ccgagaccat ttccagttct gcggaggtct gggcggtctc ttggcggaca
     2641 catttcaagc attcgacgta gaaccgtagg cccttgtccg tgggcacgcg cccggcagaa
     2701 gtgtgcggct gctcgatgaa cccaaggcct tcgagggttt tcatttcgct gcgtatcgtc
     2761 gcgctgctgc actcgagatt ggtgatctcc aggattcgtt tcgagctgac cggtacgcgc
     2821 gaagtcacgt attcatggat caccgcgtat aggatcgtac tctgtcgtcc cgttaattcc
     2881 atctttccct tcctccgaat tagcactcta atattatgag tgctaacagt ggtatgctac
     2941 catgattaga cttatttgtc aagcccgctt ttcgaagtag cggaggtttt gccgtcggtt
     3001 ttcaggacac tcaaaacagt gacttcggca tccgtgtgcg gaaagtgcag atcaagttat
     3061 ggagtggcat gaataagagg ttcatcgcgg ggtaaccgca ggctgacttc ggagcccacg
     3121 aagtctcgag atacgctttt tgtctgttta cgccgcgatg cccgtatgcg gttatcgcgg
     3181 ggtaaccaac gggaccccgc gatactgatg aagacgcacg atctgcctga gtaaaagcgt
     3241 atcgcgccgt cgcgagaaga agaagcgctt acgccgcgat gcttgtccta gcagtcctct
     3301 ccgtgttctc tgtgccctct gtgcgaaccc tgttttattt ttgagacctg ccgaacgaaa
     3361 gtccgtgtcc caggacgttc ccagagagac aggccgactg gaagtcggcc ccacgagtta
     3421 tcttcaatct tttctctgtg ttctctgtgc cctccgtgcg aaccctgttt tattttgaga
     3481 tatgccaact taaaagtaga cgtactcaga aacctgcaga acgaaagtcc gtgttcccag
     3541 gcatttccgg atgttcccag agagacaggc cgactggagg tcggccccac gagttatctt
     3601 taatcttctc tgtgttctct gtgccctctg tgcgaaccct gttttatttt tgagacctgc
     3661 cgaacgaaag tccgtgttcc caggacgttc acagagagac aggccgactg gaagtcggcc
     3721 ccacgaagag aagatgcgtt tacgctgcga tgactatgtg ggctaaaaat cgtgcgatcg
     3781 attctggcct tgtttcttga ttacaccgtc tagcttgact tgcactaatt ctctaaaaaa
     3841 acattgcaat atgctataat taatagtcga cagttggtag aacgcgccta aggaaaaggt
     3901 aagcaaattc ggtcagtgag gagagcgatt caaaaaaaca cagttaagga gagttgaaga
     3961 tgaaattgaa gagtatgcgg gcgaaacttc ttgtcttttt cattccggtt tctgttgtag
     4021 tgcttgtgac tgtagggttg attgtcgctt tgctggctag aaacgtcact tataaccaag
     4081 cagttgacct ttctgcggag acgatgacag cggcttcccg catcatagaa gagtggttga
     4141 tgggtgtgaa gaacgagatg cgaaatctgg cgaacaccga tgtggtccgt tctttctatc
     4201 ctgatttttt tatcgcaact ttccttgaag cgatccaaca aagcggggga gtttaccagt
     4261 ctattctttt agctgatacg aaaggaaagg ctatatcgga aaggggaatt gaggtcgata
     4321 ttagtgatcg aaactatttc aaagagatcg tattacaagg gaaggagttc acggttagtg
     4381 aaggccttca cagtcgattc actaatgatt acgccgtaca agtcgctcat gtcgtctttg
     4441 accgccatgg gaatcgtatt ggcctcctag gcgcctcgat ttcgttaaaa acattgagcg
     4501 aaaaggccgc aaccatcaca ctcgggaaag gggggtacac ctttctaact gacgggtcgg
     4561 ggcgggtgat cgcgcataac aggtcagacc tcattatgaa actcaacgta ctggaagcgg
     4621 agaagtcggg gtacaagggc ctggaagaag ccggaagaga gatggtacgt gggcatgaag
     4681 gcatccacaa tattgttcaa cccacgggag aaaaacaaat cttgctctat taccccgtac
     4741 caaatacgcc aaactggtcg ttagccggag tcatttcaga ggcggaagtg gaagagaaga
     4801 gtaccttcct cctcggaatc gtcgtcctgt gttttgcaat catcgtcgcg atcatcgtgg
     4861 ttatttctat gttcgtcggc acgatcatct cgaaatcttt aaataaactc gccgttcaag
     4921 tggatcaatt tggcgaaggt gacttgacca cgaccttcga agttaagggc cacgacgaac
     4981 tcgcccggat tgcgcgcgca ttgtccggga tgggccacga cctcagagag gcgatggtct
     5041 cgattgaagg aacgtcaaaa gatataaaaa ccgcgtcaac ggacttagcc gcgatctcgg
     5101 aagaacaact cgcgggttct gaagaattgt cctcgcaatc acatagcgtg aacacgaatc
     5161 tacagaatac agtagccgcg atagaagaag tggactcagg agtggaagag gtggcggcga
     5221 gcgcacagaa cgtgtcgagg acagcacagg cgttatcgat cgaaaacgac aagacagcgg
     5281 acggttctcg caggggcggg cagatggtct cggacgtcgt acgcgagatc gaagcgacga
     5341 cgcaacagac actactgacg gcagaaaagg tacagcagct gtctgagcac tcgaaaaacg
     5401 tgggtgagat tgtcgacacg atctcctcga tcgcagagca gacgaatcta cttgcgctca
     5461 acgcggcgat agaagcggcc cgggcgggag aagcgggtcg ggggtttgcg gtagtagcgg
     5521 acgaaatccg gaaattagct gaagaaagca aaaaggcgac atcgaacatt ggaactattt
     5581 tgaaagaggt acagaaaaac gccgaggaat cctatcaagc gacggacaag acagtcgtat
     5641 tggtgaaagg tgttaacgaa aagtcgcaag ccgtggagca gcagttcaaa cagttgctga
     5701 gtatggtcga aaagacgacc gtaatgatcg agaacctgtc aactacctcc gaggagcagg
     5761 gtgcggcggc ggaagaggtg gcctcggcga tgaacacatc ggcgaaatcg gcacaagaga
     5821 tctcggaacg catcggacag atgacgcggg gcatcgagca acaggctgaa ggcgcccggc
     5881 agctaagcgc agccgcggaa gagctcgaca ccctagcgga gaacctcgac acgcagatca
     5941 agcggtttaa aatatagact gttttaatta tcgtttgtaa acaaataggg cgaatgcgtc
     6001 tgggaacgtc gaacttttgt ccggcacggg tttttgtccc catggttaca tcacgcttta
     6061 gtatggcgtg ttttacctgt cttgctgctc tgtcctctat gagcgaaacc cttttttctt
     6121 ttaaacgatg ccgagcgaaa attccgcgcc cccagagtgt attcagaacg ttacctgggc
     6181 gctttccgag aaaatcttcg tgttctctct gtgttctccg tgctctctgt gcgaacgcct
     6241 tttgatccct gcc
//
LOCUS       DAOPXN010000133         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12533_length_6215_cov_5.495130, whole genome shotgun sequence.
ACCESSION   DAOPXN010000133 DAOPXN010000000
VERSION     DAOPXN010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_6215_cov_5.495130"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2272)
                     /locus_tag="PKM49_07550"
     CDS             complement(<1..2272)
                     /locus_tag="PKM49_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY82703.1"
                     /translation="MFHVHSCFQLIDSAITIERLIDRAVQSKLRYAALCDANLYGAQK
                     LFSSAHRKGILPIIGRECRTSEGLVSCFARNRLGYNLLVRYHNNLVSLASLFEADEVL
                     KVFQYPIDVETLSRYPNTYYGFTSIQRGVHDDRAVFFLPTRGFEALDEEVCHALSFLR
                     KKTQPLDEDYIQFSNQEGLVHRYPEYREEIERLFSLETYFSDYTLNVKVTIPLAPKVS
                     QAVASGQNDEAAYLLELLEEPLLRIPEPRRETYRQRVYEEWSVIRDKNFTRYLLLALD
                     FKDCCQVADAWMGPGRGSAVASLIVHLLGLTQPDPIENGLLFERFLNPDRTEPPDIDI
                     DIEDTRRPAVLEEIIQRYGRHRVAQIVTFGTFGTKNAVREIEKIPELRVLLERSGHRT
                     EIIERIGTLPRHTSIHAAGIVLSEEELGALIPLTITEEGTITQYDMNDLAEIGIVKFD
                     LLGLTALSFLREAGIELQEVDPNDPSLYGSVRPDNLCGIFQLDTVTGRKIVERFEPKD
                     FSDLRILISLNRPGPAKSGILEQIVLNREKEPDNLNLIPQVKEVLAETYGIPIFQEQI
                     MTIATRLGGLLPAEADGLRRALTKKRPELLERYEESFYSGAAERGIDRKTAETYFSEM
                     QKFAGYAFNKAHATAYATITLWMLYLKEHYPLRFYRGLFNRHHASAEKLFQILNECGR
                     RGLTLEPPDLEKSDVETQVEGEGLRLGFQLFKGVNPAFFNSVIQERQTLRYPNAEELF
                     LGLSTGLLDDQTAYRLY"
     gene            complement(2406..2482)
                     /locus_tag="PKM49_07555"
     tRNA            complement(2406..2482)
                     /locus_tag="PKM49_07555"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2446..2448),aa:Met,seq:cat)
     gene            complement(2489..2565)
                     /locus_tag="PKM49_07560"
     tRNA            complement(2489..2565)
                     /locus_tag="PKM49_07560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2529..2531),aa:Met,seq:cat)
     gene            complement(2584..2672)
                     /locus_tag="PKM49_07565"
     tRNA            complement(2584..2672)
                     /locus_tag="PKM49_07565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2636..2638),aa:Leu,seq:taa)
     gene            2825..3595
                     /gene="fdhD"
                     /locus_tag="PKM49_07570"
     CDS             2825..3595
                     /gene="fdhD"
                     /locus_tag="PKM49_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNY82704.1"
                     /translation="MAITKTIQIDRITDGVLETLEDVVVVERAIDVFIENRKAFSATL
                     SDSSIDLLLIGRLFSEGIIQKATDILQISSVGDRYDVFLRGEGAQALGKRGGSERDPE
                     RACENEHKQVPPNTEKKEDAEKYKIERLLSLMKEFLGGAAVFQETGGVHRCGLSDGHT
                     LLFAVDDIGRHNAVDKTIGFSLRQEFDPTDKLLYTSGRTSLDIVVKAWTAGFAGIVSK
                     SAATDLAVETARRHGLLLIGFARGNRCNLYAGENRVEN"
     gene            4037..5377
                     /locus_tag="PKM49_07575"
     CDS             4037..5377
                     /locus_tag="PKM49_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY82705.1"
                     /translation="MNLYKRLFVVALPLTIQQLLSSSVNLLDTLMIGQLSEVAIAAVG
                     LSNQFYFVYALLLFGLSSGGGIFIAQYWGAKDTVNISKTTALMLTVAALLLIPFFILA
                     VFFPSFTIRLFSPDARVIELAVSYLRVIGYSLLPAAISQVFYSVLRSTEHVFIAMVFS
                     GITMGLNAFFNWILIFGLFGLPALGVFGAGLATLIARLAELGMVMAVIYGKSLPGKLG
                     FIHFQSLTKRFFKRFMVYVLPAMGNELSWSMGITLYAVIYSHIATDVLAAHRMMQSIE
                     SFTFAFLFSIAHATAIIVGKELGKRKFDEAIEVANKSIRINFILSLITGLTIFAFSGW
                     MIGVYNVKPEVRGLAYLILGLSLLFLPVKSLNALLVVGIFRAGGDTKYTLFVEGGTLW
                     ALGVPLTAFVGLILEAPFPWVFSMSLLEEGMKGLILSIRYRRRIWVRNVIGENI"
     gene            5374..5706
                     /locus_tag="PKM49_07580"
                     /pseudo
     CDS             5374..5706
                     /locus_tag="PKM49_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5725..6192
                     /locus_tag="PKM49_07585"
     CDS             5725..6192
                     /locus_tag="PKM49_07585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNY82706.1"
                     /translation="MEVPGSYESQLQRARDILTTASDFSKATQKLCNELRQRNPLFDW
                     VGFYWINPDNARELNLGAYSGEPTEHVTIPVGKGICGQAAERKETFVIQDVRLEENYL
                     SCNPAVLSEIVIPIFSKGEVIGELDIDSHTEAAFTDEHREFLEEVVKIIETRL"
BASE COUNT         1466 a         1575 c         1663 g         1511 t
ORIGIN      
        1 ggtagagcct ataggcggtt tggtcgtcta ataaccctgt ggatagtccc agaaacagtt
       61 cttcggcgtt tgggtatcga agtgtttgcc gctcctgaat aacggaattg aaaaatgctg
      121 ggttcactcc cttgaatagc tgaaacccca accgtagccc ctctccttcc acctgagttt
      181 ctacgtcgct tttttctaga tcggggggtt ccaacgtcag accacgacgc ccacattcgt
      241 tcaggatttg gaaaagcttc tctgcgcttg cgtggtgacg gttgaacagc ccgcgataga
      301 aacgtaacgg atagtgttcc ttgagataga gcatccacaa ggtgatcgtc gcgtaggcgg
      361 tggcgtgggc cttgttgaag gcgtagcccg cgaatttctg catttcggaa aaataggttt
      421 ccgctgtttt tcggtctatc cctctttccg ctgctccgga atagaaagat tcttcgtaac
      481 gctccagcaa ctccggacgt tttttcgtca acgcgcggcg cagaccatcc gcttccgctg
      541 ggagaagccc accaaggcga gtcgctatcg tcataatctg ttcttgaaag atcggaatcc
      601 cgtacgtttc ggccaacacc tctttgacct gcgggatcag gttaaggtta tccggttctt
      661 tctccctgtt taggacgatc tgttcgagta tcccgctttt agccgggccc ggtcgattca
      721 gggagatgag tatccgaagg tccgaaaagt ccttcggttc gaaccgctcg actattttcc
      781 gcccggtcac ggtgtccagc tgaaagatcc cgcaaagatt gtcgggtcgg acggaaccgt
      841 agagagaggg atcgttcgga tcgacctctt gaagctctat ccccgcttcg cgtaaaaatg
      901 acaaagccgt cagccccaac agatcaaact tgactatacc gatctctgcg aggtcgttca
      961 tatcgtactg ggtgatcgtc ccctcttcgg tgatcgtgag cgggatcaac gcgcccagct
     1021 cttcttcaga caaaacgatg cctgcggcgt gtatcgaggt gtgtcgaggt aatgttccga
     1081 ttcgttctat gatctccgtc cgatgccccg acctttctaa caagactcgc aactcgggaa
     1141 tcttttcgat ctctcgaacg gcgttcttcg tgccaaaagt gccgaaagta acgatttgcg
     1201 ccaccctatg gcgaccatag cgctggatga tctcttcgag caccgccggc ctacgggtgt
     1261 cttcgatgtc gatatcgata tccgggggct ccgttcgatc cgggttcagg aaccgctcaa
     1321 agagtaaccc gttctcgatc gggtcaggtt gggttaagcc gagtagatgg acaatcaaag
     1381 aggcgacggc ggatcctctt cccggaccca tccacgcgtc ggctacctga cagcagtcct
     1441 tgaaatccaa ggcgagcaag agataccgag tgaagttttt atcccgtatg acagaccatt
     1501 cctcataaac gcgctgccga tacgtttcac gtcggggttc aggtatccga agcaacggtt
     1561 cttcaagaag ctccaacaga taggccgcct cgtcgttttg acctgacgcc actgcctgcg
     1621 acactttcgg agctaaggga atagtgacct ttacattgag cgtatagtca gaaaaatacg
     1681 tctcgagaga aaagagacgc tcaatctcct cacgatactc tggatagcga tgaacaagcc
     1741 cttcttgatt tgagaactgg atgtagtcct catctagagg ctgtgttttc ttccttagaa
     1801 aagacaacgc gtgacagact tcttcgtcta gcgcctcaaa accgcgcgtt ggaaggaaga
     1861 acacggcgcg atcgtcgtga acgccccgct ggatagaggt gaatccataa taggtgttcg
     1921 gataacgcga caaggtctcc acgtcgatcg ggtattgaaa gaccttgaga acttcgtcgg
     1981 cttcgaatag gctggcaagg gaaactaagt tgttgtggta tcgcaccaaa aggttataac
     2041 ccaagcggtt tcgggcgaag caagagacca gtccttcaga agttcggcat tctcttccga
     2101 tgatcgggag gattcccttc cggtgggctg acgaaaatag tttttgcgcg ccgtacaggt
     2161 tggcgtcgca caatgcggcg taacgaagct tcgattgaac ggccctatct ataagccttt
     2221 ctatggtgat cgcggaatct atgagctgga agcaagaatg gacgtggaac atagcccttc
     2281 accccgtttt tcggtgcgat gtgttcttcc ttccgtacga agtggtccac tatgtgcttt
     2341 tctgcacctc taagcaaaaa ccccgatcac tcggggttcg ctgtgtccgc acctggttag
     2401 gcttgtggtg gcggcggtca gagtcgaact gacgacacta cgggtatgaa ccgtatgctc
     2461 tagccaactg agctacaccg ccgtaagctg gtcgcgggga caggatttga acctgtggcc
     2521 ttcgggttat gagcccgacg agctaccaga ctgctccacc ccgcaatact cgctcgcaat
     2581 tcttggtgcc gagggcggga attgaacccg cacgaggctt acgcctcaac ggattttaag
     2641 tccgttgcgt ctgccgattc cgccacctcg gcgagcacgc atactatacc acaaaccggt
     2701 cggattgtca atatcgcttt caaggctcct acgcggggaa tccttcacta caagtgaaga
     2761 cgaaattatt tagagtcctt tcgtgtataa tactgagcgt tcgaaaaagg cggtgagtct
     2821 aggcatggcg atcacaaaga caatacagat cgacaggata acagatggcg ttttagaaac
     2881 tctcgaagac gtggtcgttg tagagagggc gatagatgtc ttcatcgaaa accggaaagc
     2941 cttttccgcg acactctctg actcatcgat cgaccttttg ttgatcggac ggctcttttc
     3001 cgaaggtatc atccaaaaag ccacggatat actacagatc tcgtccgtag gggatcgcta
     3061 cgacgtcttt ttacgtggag agggcgcaca ggcccttggc aaacgtggcg ggtcagaaag
     3121 agatcccgag cgggcttgcg aaaatgaaca taaacaagtc ccacctaaca cagagaaaaa
     3181 agaagacgcg gagaaataca agatcgaaag gttgttgtct ttgatgaaag agttccttgg
     3241 cggagcggca gttttccaag agacgggtgg cgtccatcgt tgcggtctgt cggacggaca
     3301 tacgctcttg ttcgccgtag acgatatcgg gagacacaac gctgtcgaca agaccatcgg
     3361 cttttcgtta cgtcaagagt ttgaccctac ggacaagtta ctctatactt ccgggagaac
     3421 gagcttagat atcgtcgtta aggcgtggac ggcaggtttc gccggaatcg tttcaaagtc
     3481 ggccgccact gacctggccg tcgaaaccgc gcgccgccac ggattgttgc tgattggttt
     3541 cgcccgcgga aacagatgca atctctacgc gggcgaaaac cgtgtggaga actgatccga
     3601 agactggctc agataagacg agcctttcct tgacgcaacg aaagacaact gacggatagc
     3661 gtcacaagat gacggccaca gaatgagagt agctattccg gcgggaaaga cggtttttcg
     3721 cataaaactt gcctggaagc tgaaacgaag atacctgtgg gaataatatc gaggtgtgac
     3781 aaaaaaaacg gaatgaggat gcccgtgtgt gttatcgcag ggtaagtagt ctttacgacc
     3841 ccgcgatccg ccctatgccc ttctcaaaag cgtatcgcgg catcgcgaaa agaaggggcg
     3901 cttacgccgc gatgactaca tagactaaaa atcgtgcgat agattctggc cttgcttctt
     3961 gaaggcacct cataactgga cacacacgaa gaagctcaaa gaagagttac tcaaagaaaa
     4021 gaggttttcc actgagatga acttgtacaa acggttgttc gtggtcgccc ttcccctcac
     4081 tatccagcaa ctcctctcga gttccgtcaa tttgctcgac acactgatga tcggacaact
     4141 gtcagaagtc gccatcgctg ccgtgggatt gtccaatcaa ttctattttg tgtacgcgct
     4201 tctgctcttc ggtttgagta gcggtggcgg aatcttcatt gcccaatact ggggggcgaa
     4261 agacaccgtc aatatttcca aaaccactgc gctgatgctc acggttgccg cgttattgct
     4321 cattcccttt ttcatattgg ccgtcttttt cccttcgttt acgatccgtc tgttctcacc
     4381 cgacgcgcgc gtcatcgaat tggctgtgag ttatttacgg gtgatcgggt attcgttact
     4441 accggccgcg atctctcaag tgttctattc ggttctgcga agcacggagc acgtcttcat
     4501 cgcgatggtt ttcagcggga tcacgatggg tttgaacgcg ttcttcaatt ggattctcat
     4561 cttcgggctc ttcgggttgc ccgccttagg agttttcggg gcgggtttag cgacgctcat
     4621 cgccagactc gcggagctcg gtatggtgat ggcggtgatc tacggaaaat cgctgcctgg
     4681 aaaactgggc ttcatccatt tccagagcct gaccaaacgt ttctttaaac gtttcatggt
     4741 gtacgtcttg cctgcgatgg gcaacgaatt gtcgtggtct atggggatca ccctctacgc
     4801 ggtgatctat agccatatcg ccacagacgt gttggccgcg catcgcatga tgcaaagcat
     4861 cgaaagtttc accttcgctt ttttattctc aatcgcccac gcgactgcca tcatcgtggg
     4921 gaaagaatta ggaaagagga aatttgacga agccatagag gtcgcaaaca agagcatccg
     4981 gatcaacttc atcctctcgt tgatcacggg gttgacgatc ttcgccttct cgggttggat
     5041 gatcggagtc tacaatgtga agccggaggt caggggattg gcctatttga tcttggggtt
     5101 gtcccttctc ttcttacccg taaagagcct gaacgcgttg ctcgttgtcg gtatcttcag
     5161 agccggcggg gataccaagt atacgttgtt cgtggaaggc gggactctct gggcgctcgg
     5221 agtaccctta accgcctttg ttggcttgat cttagaagct cccttcccgt gggtgttttc
     5281 gatgagccta ctcgaagagg ggatgaaggg gctcatccta tctatccgtt atcgtaggcg
     5341 gatctgggtt cgtaatgtga tcggcgaaaa catatgagaa aacggaggtt acctgtgacg
     5401 gaaaaagatc ggtttcagag agaacgagaa cgtttgaacc agttggtgat gaaatacgcc
     5461 aacaaagaga cgaagcgctt cttcagcctg gaccgccaga cctacgaaaa gggcgcgttg
     5521 gacgagaaga cgaaagagtt tgcgggattg gtcgcctctt tggtccttcg atgtgacgat
     5581 tgcattttct atcacctcat acgatgccga gaagccggag ccacatccga tgagatcatc
     5641 gaaatcctca acatcgggct catagtaggg ggctcgatcg tgatccctca ccttcgccga
     5701 gccctggccc gttggggcga gttggtggag gttcccggat cttatgagtc gcagctacaa
     5761 cgagcaagag acatcctgac cacggcctcg gatttctcaa aagcgacgca aaagttatgc
     5821 aacgaattgc ggcaacgcaa cccgcttttt gattgggtgg gattctactg gatcaatccg
     5881 gacaacgccc gtgaactcaa tctcggcgcc tactccggcg agccgacgga gcatgtgacg
     5941 atacccgtcg gaaaagggat ttgcggacag gcggcggaaa gaaaggagac tttcgtgatc
     6001 caagacgttc ggctcgaaga gaattacctc tcctgtaatc cggccgttct gtcggagatc
     6061 gtgataccga tcttttcgaa gggagaagtc atcggagaac tggatatcga ttcgcacacc
     6121 gaagccgcat tcacggacga acaccgagag tttttggagg aggtcgtgaa aatcatcgaa
     6181 acacgactat aaagaatttt tttggtgatt ctacg
//
LOCUS       DAOPXN010000134         6187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12603_length_6187_cov_5.191781, whole genome shotgun sequence.
ACCESSION   DAOPXN010000134 DAOPXN010000000
VERSION     DAOPXN010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12603_length_6187_cov_5.191781"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..868
                     /locus_tag="PKM49_07590"
     CDS             401..868
                     /locus_tag="PKM49_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNY82707.1"
                     /translation="MEIRVNVNGVEKRWNVEPSELLVDVLRDNGYKSVKKGCGSADCG
                     TCTVLVDGRAVLSCSVFAASVSGKTIETLEGLGTDQQLHPIQQAFLDAAAFQCGFCAP
                     GLIMATKELLEKEPALTEDQIRHELSGNLCRCSGYENQVKAISGLMDKKREGA"
     gene            873..3203
                     /locus_tag="PKM49_07595"
     CDS             873..3203
                     /locus_tag="PKM49_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNY82708.1"
                     /translation="MPNDSKTFRIVGQNKEKVDARGILRGKPVYTEDFYPRDALIVKV
                     VHSPYAHALITRIDKSAALDIPGVVEILTYEDVPRIPYTRAGQGYPEPSPYDTVLLDH
                     KMRFVGDTAALVIAENERAARMGVKALKIDYEVLEPVLDFEKSMSPSAPIIHDQKDSK
                     GIYEPSRNIASHYETTIGDVEGLLARSPVQIEATYYYPKSQQVALENHCTYAYLDPQE
                     RLTLVSSTQVPFHGRRILSKILQTPPGKIRVIKPRIGSGFGSKQGLFTEGYAALACLK
                     TGKPVCLSFNREETFTCTYTRHDMRVTVRLGAELDGRLNVIDMYSLSNTGAYGDHALT
                     VAMGCGSKTLPLYNKVKAVRFVTDIVYTNLPNAGAYRGYGAPQGLLALDGALDELAAR
                     LNRDPLEIKAINSIREGETSPIFQIMGEGREGVPQIAASCKLGECIEIGKVAFDWAKK
                     RKNAGWDRRTVDTVRGVGCALAMQGSGIAKVDMASATLKMNDDGTFNLLVGATDLGTG
                     SDTILSQIAAEALGIPVENIIVYSSDTDMTPFDVGAYASSTTYVSGNAVLDAANKVKA
                     SIRRAAAALFEIDEELVEVTDGCVKNLQNGKTLTFAELCTLCFYGANQHQICETGSYV
                     GDESPIPFMATFIEVEIDLKTGKITPIETLSVVDCGTALNPALAEGQVKGATLQALGT
                     ALFEELKFSETGKPINASLFRYKILDRSSFGKVTALLAESYEPTGPFGAKSVSEIGIN
                     TPTVALLNAVYHATGIRFHRLPLAPQTVFNALKGKA"
     gene            3261..3806
                     /locus_tag="PKM49_07600"
     CDS             3261..3806
                     /locus_tag="PKM49_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82709.1"
                     /translation="MALYKAFDPKVEVNGETVLGFVNGLGAFKRQALALLKEQGIDDP
                     TPGKWYSQQAWLNGFKMIGEKFGDATLKKIGESVANSAIWPKEIKTIVDALKSIDVAY
                     HMNHRNGKIGNYAFTKIDAETGQMVCSNPYPDQFDLGLIRTVAEKFKPSQATLMVSID
                     ESQSTRTKGAETTTFIIKGLK"
     gene            complement(4369..4608)
                     /locus_tag="PKM49_07605"
     CDS             complement(4369..4608)
                     /locus_tag="PKM49_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82710.1"
                     /translation="MYIGENRADGFGVEGAHSHFQLSKLSFRSDHRQLRELGEAGDRL
                     EQRIDDLYAMRVISYIRLVGEAEPVGSFYHHIRKV"
     gene            4873..5213
                     /gene="rnpB"
                     /locus_tag="PKM49_07610"
     ncRNA           4873..5213
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKM49_07610"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            5240..6139
                     /locus_tag="PKM49_07615"
     CDS             5240..6139
                     /locus_tag="PKM49_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNY82711.1"
                     /translation="MVYHLLLWLTVCFWGVSFVATKVVVSVFPPFFAAILRFLLAWVF
                     LWLFTRKRAYGKRKDLVLAGLWGVTLYFVFENLGLVYTLPTNAVLIISLVPLLNLLYL
                     SLFRKQRLTILQISGAVIAFTGVCIVFLNGNIYLRLNPVGDLLIFGSAISWVFYTHYI
                     LRVEEEKSQKNGDALAVTRSITGWGTLFLIPFGALEHLIGRSQWSLQSLGWPFFIGLL
                     FLSLCCSSVGYYLWNTAIRHLGSRYTTNALYLMPIITGVAEALILGSIPNRYTLIGAF
                     FVLLGLYISEQKKMTEKGARSTE"
BASE COUNT         1520 a         1552 c         1718 g         1397 t
ORIGIN      
        1 cccacgaaga gaaggggcga ctacgccgcg atgactacgt ggactaaaaa tcgtgtgatc
       61 gattctggcc ttgtttcttg agtacacccc ataacttgac acacacacta atcgggagtt
      121 ctttggttaa gagatataat tccagattga tgtgaagccg aataggtggg aagaacgcaa
      181 cgaccccgta agggcggtgg tgttctcttt aatgagtggc ttcgtgcgct gtctttcgtc
      241 ggcgctttga gaaccaattt tcgagaataa tgtataacac atccttgcgt cgttggggac
      301 atttgtatca ttcaattcag tgttatgcgc aaggtccatc gcctcaaata gagacagtca
      361 tgttataatc cgccttgagg actaccaaag gagggatact gtggaaattc gcgtaaacgt
      421 aaacggcgta gaaaagagat ggaacgtgga accgagcgag ctgctcgtcg atgtcctccg
      481 ggacaacggg tataaaagtg ttaaaaaggg ctgtggaagc gccgactgcg gcacctgcac
      541 cgtcttggtt gacggacgcg ccgtactctc ctgctcggtt ttcgccgcgt cagtttcggg
      601 gaaaacgatc gagaccttgg aagggttggg caccgaccag cagctgcatc cgatccaaca
      661 ggcattcctg gacgccgctg cttttcagtg tggcttttgc gccccgggtt tgattatggc
      721 gaccaaagaa ttgctcgaaa aagagccggc gttgaccgaa gaccagatca gacacgaact
      781 gtcaggcaat ctctgccgat gttccgggta cgagaaccag gtgaaagcta tcagcggatt
      841 aatggacaaa aaacgcgagg gagcgtgagc cgatgcctaa cgattctaaa acattccgta
      901 tcgtcggcca aaataaggaa aaggtggatg cacgaggtat tctgagagga aaaccggtct
      961 acacggaaga cttttatccg agggatgcgc tgatcgtgaa ggtcgtgcat tccccgtatg
     1021 cgcacgcgct catcacgcgt atcgacaagt cggccgcctt ggacatcccg ggggtcgttg
     1081 agattttgac gtacgaggat gtcccccgta ttccctatac gagggcgggg cagggatatc
     1141 ccgaaccatc accttatgac accgttctgc tagaccacaa gatgcgtttt gtcggggaca
     1201 ccgccgctct ggtgatcgcc gaaaacgaac gcgccgctcg aatgggcgtg aaagccctca
     1261 agatcgacta cgaggtcttg gagcccgtct tggatttcga aaagagtatg tccccttccg
     1321 cacccatcat ccacgatcaa aaggattcca aaggcattta cgagccttcg agaaacatcg
     1381 cctcacacta cgaaacgact ataggagatg tggaaggtct tttggcgcga tctcccgtgc
     1441 agatagaagc gacctattac tacccgaagt ctcagcaggt cgctctggaa aatcactgta
     1501 cctatgcgta cttggatcct caagagcgac tgaccctcgt ctcgtcgaca caggtgccgt
     1561 tccacggccg aagaatcctg tcaaagatcc tacagactcc ccccggtaag atccgggtca
     1621 tcaagccccg gataggcagc ggtttcggtt ccaaacaagg cttgttcacg gagggatatg
     1681 cggctctggc gtgcttgaag actggaaaac ccgtctgttt gtctttcaac cgagaagaga
     1741 ctttcacctg cacctatacc cgacacgata tgcgagtgac ggttcgacta ggggcggagc
     1801 tggacgggcg tttgaacgtg atcgacatgt actcgctgtc caatacgggc gcttatggag
     1861 accacgcgct caccgtcgcg atggggtgcg gttcgaaaac gcttcctcta tataacaaag
     1921 taaaagctgt ccggttcgtc accgatatcg tgtacacgaa tttacccaat gccggcgcat
     1981 accgcggtta tggggctcct caaggattgc tggcactcga cggcgcgctg gacgaattgg
     2041 cggcgagact caatcgtgac ccgctcgaga tcaaggccat caactccatt cgagagggag
     2101 agacctcacc gatcttccag attatgggcg aaggtcggga aggggttcct caaatcgcgg
     2161 ccagctgtaa attgggcgaa tgtatcgaga tcggtaaggt cgctttcgac tgggcgaaga
     2221 agagaaaaaa tgccggatgg gatcgacgaa cggtggatac cgtgcgcggc gtaggctgcg
     2281 ctttggcgat gcagggctcc ggaattgcga aagtggatat ggcttcggcg actctaaaaa
     2341 tgaacgacga tggcaccttc aacctgctgg tgggcgcgac ggatctaggg accggatcgg
     2401 atacgatcct aagccagatc gcggccgaag cccttgggat cccggtggaa aacatcattg
     2461 tgtattcgtc agatacggat atgacgccat tcgacgtggg cgcctacgct tccagtacga
     2521 cctacgtttc gggaaatgct gtcctggacg cggcgaataa ggtgaaggct tctatccgcc
     2581 gggcggcagc ggcgctcttc gagatcgatg aagagttggt cgaagttacg gatggttgcg
     2641 tcaaaaacct ccaaaatggt aaaaccctga ccttcgcaga gctctgtacc ctctgtttct
     2701 atggcgcgaa tcaacatcaa atctgcgaaa cggggtctta tgtcggcgat gaatcgccta
     2761 taccctttat ggcgaccttc atagaggtcg agatcgattt gaaaaccggc aaaatcaccc
     2821 ctatcgaaac gctcagcgtc gttgattgcg gcaccgccct taatccggcg ctcgcggaag
     2881 ggcaggtcaa aggggcgact cttcaagcct tgggtaccgc tctgttcgaa gagctaaagt
     2941 tcagtgaaac tgggaagccg attaacgcct cgcttttccg ttacaagatt ctggatcgca
     3001 gcagcttcgg caaagtcacc gcgcttctgg ccgagagcta tgaacccacg ggccccttcg
     3061 gagctaaaag cgtttcggag atcggaatca atacgcccac cgtcgcgctg ctcaacgcgg
     3121 tctaccacgc gactggcata cgtttccatc gtttgcccct cgcacctcag accgtgttca
     3181 acgccttgaa gggaaaggcc tgaaagcgcc acgaaaatcg ggatgatttt tctattatat
     3241 acggaaagga gtggaggtta atggcgcttt acaaagcgtt tgatccgaaa gtggaagtca
     3301 acggagagac ggtgttgggg tttgtgaacg ggttaggagc cttcaaaagg caggcattgg
     3361 cgctgctcaa agaacaagga atagacgacc cgactccagg aaaatggtat tcccaacaag
     3421 cgtggctgaa tggcttcaaa atgatcggag aaaagttcgg agacgctacc ttgaagaaga
     3481 tcggagagag tgtcgcgaac tcggcgatat ggccgaagga gattaaaacg atcgtggacg
     3541 ccttaaagtc gatagacgtc gcctatcata tgaaccaccg aaacgggaaa atcgggaact
     3601 acgccttcac caagatcgat gctgaaacgg gccagatggt ctgttcgaac ccctatccgg
     3661 atcagttcga tctcggattg atccgaacgg tagcggaaaa atttaaaccg tcgcaggcga
     3721 cccttatggt cagtatcgac gaaagtcaat cgacgagaac gaaaggagcg gaaacgacga
     3781 ccttcatcat caaagggctc aaataacggc ccggaaaacc cctgtgaaca gggtattttt
     3841 cccgcccagc ccctcgcttt ttgagaggtt tagagggtaa ggtaggaacc atttccggtt
     3901 gagcgaacac acccgaaaaa gaggggtaga ttcgccggtc gacaggcgtc gttgtagcgc
     3961 gtgactattt gacgagtgag ggacagaata cgaacatcta tctagcgaac ctacgagaag
     4021 gagccgtgca tgaaatccaa tacctataag atgtttataa ccgatctgga cggcacgttg
     4081 ttggacgacc ggaagaacat ctcggaagag aacatcatcg cgctaaaaaa attgaccgac
     4141 catcaagtat tcatcacggt cttcaccggg agaaactacc tttccgccaa acgcttcatc
     4201 gacgcgttgg gcatcgatat tcccgtggtc tttcagaacg gcgcatttat tatgaaccct
     4261 aagtcgaaag aggtgcttta ccaatcgatg ctaccgggtc cggaagcgcg gtctatcacc
     4321 gaaatcgcgt tggcctccgg cttggaagtg gtcgcctata agggatttct agaccttccg
     4381 gatatgatgg tagaacgaac cgactggctc ggcttcccct acgagtctta tatacgaaat
     4441 aactcgcatc gcatacagat cgtcgattcg ctgctcgaga cgatccccag cttctccgag
     4501 ctctctcagc tggcggtgat cggaccggaa ggagagcttc gagagttgga aatgagagtg
     4561 cgcgccctct accccgaaac cgtcagcccg attctctccg atgtacaaaa tgggagcggt
     4621 ttcctagagt ttttcgggcc gcaggtttcg aaagggatcg ctttggaaaa ggttttagcg
     4681 agatttcata tagaacctgg gcataccgtc tttatcggag acaactacaa cgatttggaa
     4741 ttgatggaga aggtgggctt tccggttgcg gtagagaact cgccggagcc gataaagaaa
     4801 cgttgccggt tggtcgtcac aggaaacaac gagagcggcg tggccgacgc cgtgaatcgt
     4861 atttttttca atgaagaggg acaggtggcc gcgggtagga aatagcctga ggaaagtccg
     4921 gactctgagg gcaaggcgcc ggttaatcgc cggaggggga aacctctgga aagtgctata
     4981 gaaaagaaaa ccgccgcaag gcaaggatgg aacggtgagg taagagctca ccagcgacag
     5041 agtgatctgt cggcttggta aaccccgcct tgagcaaggt caagcaggaa aggtgcctgt
     5101 gagtgaaggt gtgacctcgc aggggaaggt ggcccgccga atcctttccg ggtagaccgc
     5161 aaagagaaat ggccactcaa cgacagaatc cggcttatcg tccctcttta tttcgtatgt
     5221 ggtatgagaa ggagcgacca tggtctatca tttgttgctt tggttaacgg tttgtttttg
     5281 gggagtctcg ttcgtcgcta cgaaagtggt cgtgtcggtc ttccctccat tttttgccgc
     5341 cattttaagg ttccttctcg cctgggtatt tttatggttg tttacgagaa aacgggcata
     5401 cgggaaacgt aaagacttgg ttctcgccgg gttgtggggc gtcaccctct attttgtttt
     5461 cgaaaacctc gggttggttt ataccttgcc gaccaacgcc gtcctcatca ttagtctcgt
     5521 cccgctgttg aacctgctct acctctcctt gttccgtaaa caacgtttaa ccatcctgca
     5581 gatctcgggc gcggtcatcg ccttcaccgg cgtctgtatc gttttcctca acgggaatat
     5641 ctacctgcga ctgaacccgg ttggtgacct cctgattttc gggtccgcga tctcttgggt
     5701 cttttacacc cattacatcc tgcgagtgga ggaagagaaa tctcaaaaaa acggcgacgc
     5761 cttagccgtc acccgctcga tcaccggttg gggaacgctt ttcttgattc cttttggggc
     5821 cttggagcac ctcatcggtc gctcgcaatg gtcacttcag tcgttgggtt ggcccttctt
     5881 catagggttg ctgtttctaa gcctgtgctg ctcgagcgtc ggctactatt tatggaatac
     5941 ggcgatacgc cacttgggct caaggtacac cacgaacgcg ctctacctga tgccgatcat
     6001 cacgggggtg gctgaagctc tcatcctcgg gtcgattcca aatcgctaca cccttatcgg
     6061 cgcgtttttt gtgcttctgg gcttgtatat cagcgagcag aaaaaaatga cggagaaagg
     6121 cgcccgctct acggaatgat tctctacaag ctgcggaaga tgcgaaacga agaattggtc
     6181 ctatacg
//
LOCUS       DAOPXN010000135         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12627_length_6179_cov_5.592423, whole genome shotgun sequence.
ACCESSION   DAOPXN010000135 DAOPXN010000000
VERSION     DAOPXN010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12627_length_6179_cov_5.592423"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..1419)
                     /locus_tag="PKM49_07620"
     CDS             complement(268..1419)
                     /locus_tag="PKM49_07620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HNY82712.1"
                     /translation="MKTYTTLIATVGASLAGNISKLPDFNEDFTKGVVEPIVQQMLSR
                     SWGDPETATVYGSEINSTVSLIKHFDKLDAGTFDPINLYFFVSDTPVGEKIGSILKTF
                     FEKDSELRFRNVQVVKIAKLSDTDEYAFGKEGLRNLVREFADVARKHRYGLAVNATGG
                     YKAQIAFALALGQAMKFPVFYRFERFDHIIKMPPLPVALDWQIYLKHHALLDTLEEST
                     AFSEKTDLPTYGFRNYAAIPEELKTFIEREKIDAEHYLGLSPMGQVYIESARELLTVE
                     TPLLESIKAPEDKPKYIQDESHALKFAKKHALYEKISALSFVDKVVGFQFSPNFELSG
                     FKARFQGDEILFEYGNKGGTHRFKIYTTARNSRERLAALESLKEAAQEW"
     gene            complement(1433..4276)
                     /locus_tag="PKM49_07625"
     CDS             complement(1433..4276)
                     /locus_tag="PKM49_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82713.1"
                     /translation="MSIEREKKYRLLSDVNPQGASALKKRLKEETLKRNVRKTAVVQW
                     YLECGANTEIRLRLEIHRERNSFRHVWTYGKKRDTDDPDLREELEETIDLEKLASGQY
                     PPDEFPKLLELREGIEALQDYPCVIKTRTILADDEEKEVVFDEFLHPDDVSAMIEIEL
                     KSLELPDATFEKTLSEFGLSDCVEEITRRQTSKNRDLAKKREPDVKNPVHSLILTLQN
                     RLKGPVIVAVLQGKSLESNIEKAIRAESSQNNVKANISDLTYPYEKYGETEFKPKGRT
                     YGIPIKEILDLEAEAPLAHECVRGLSAELDSLFAIEKNGYEIDEVRYFLFPEKDGAFE
                     DEKNRCPKLYPYLKKLTQRVFHNVTVSSYSHSYAANDPESVYRSFKETWQAFEGLERN
                     NGGREIVFDSTGGHKIIGIIAALYFQFSKKPFYYVQADSDVLYKFPPAPINWDILQID
                     ESHAFYRQINGNRISYVQYLQVPQPLRNIFNSIAPEPKEAEPILTSLPIDRILSKYED
                     SRKVPFGYGEEFLDFLDDEKRKAWIRDKILSRWSLQWMGDQIPETVEHSQRHSKRLME
                     FTVNLINTIGEETFLKGIPRTHIKDFYFILAIAMNIHDLGHTNNLWRFGNGQVLHLDG
                     LPNIVRDLHNELTVQMIDGSDEDQRFRLLEGLEEFDPTGDIKKALVLVSRYHRGHMPI
                     DRPAAVEKTLDKDFVSIFELHCPPLADVCEEVFPGKPEWRAMVIALARWLKFIDGTDV
                     QADRTLIPEYSKIRCERTKYESLELIEELLRFPNPCIALNGLKSKLLAAKRQLKLYNP
                     DHCDASISTNLDDIGKTLEKTVYETVADAIYSANGNPRISISYDIRTLARIAFKIRQF
                     VHFETHNAIEVVFPRFFKEKTLAKRGDESKTKMLFLNYVLRGDQSQALLESVKSKVKK
                     DVEEEFKKAGICKLQGIEHLEVEFYEQPSSNPE"
     gene            5637..>6179
                     /locus_tag="PKM49_07630"
     CDS             5637..>6179
                     /locus_tag="PKM49_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82714.1"
                     /translation="MPFGIDFYMRMTSGITRFLRENLKDPEIAWTLEAANRLCTSDTL
                     QSARCALVSPRFLQDRALSFEHLVLLPDEEGVYTYRKKRYYSPFTLFEKGVAFDAPVS
                     FKSEEAKRTPPPKRMDLEELYVMPGYYLHLALSLSRFLRSFWDDDKNTRKAIEEWVFE
                     RYVDFLSSNLSTVPLKKKYHP"
BASE COUNT         1331 a         1547 c         1531 g         1770 t
ORIGIN      
        1 ggacaggttt ctcttggaaa aagaaaaggc atgccggact aaagcctggc gctccccggc
       61 gtttccaatc gcagcatcgc ggcgtaagcg cttcttcttt tcgcgacggc gcgataccca
      121 tttttaaaga caaagggcgg atcgcggggt cgtaaaatgc acttaccccg cgataaacct
      181 cgtagacatc gcgacacgct tctgcaaatc ccttccggac tgaattcggc gttcccgggt
      241 ggtcccaggg gctgaggatg atgcgtgtta ccactcttgg gcggcctctt tcaaagactc
      301 taaggcagcg agtcgttctc gcgagttccg cgcggttgta tatattttga accggtgtgt
      361 gccccctttg tttccatatt cgaataagat ttcgtctcct tggaagcgcg ctttgaaccc
      421 gctcagttcg aaattgggtg agaattggaa tccgaccact ttgtcgacaa aagaaagcgc
      481 cgatatcttt tcgtagaggg cgtgtttttt tgcgaatttc agggcgtgtg attcatcttg
      541 tatgtatttg ggtttgtcct cgggcgcttt aatcgattca agaagcgggg tttcgacggt
      601 caggagttcg cgcgcggact ctatgtacac ctggcccatc gggctgagtc ccaaatagtg
      661 ctcggcgtcg attttttctc gttcgataaa agtcttcagc tcttcgggga tggcggcgta
      721 gttgcgaaat ccgtaggtgg gcagatcggt tttttctgaa aaggccgtcg attcttcgag
      781 agtgtccagt aatgcgtggt gtttgagata gatttgccaa tccagcgcca ccggtaacgg
      841 gggcattttt attatatggt caaaacgttc gaaccggtag aacaccggga atttcatcgc
      901 ctgccccaac gcgagggcga atgcgatttg cgctttgtag ccgccggtgg cgttgacggc
      961 cagcccatag cggtgttttc tcgcaacgtc ggcgaattcg cgcaccaggt ttcttaaccc
     1021 ctcttttccg aacgcgtact cgtcggtgtc gctgagcttc gctatcttaa cgacctgtac
     1081 gtttctaaaa cggagttcgc tgtctttttc gaagaacgtc ttcaggatgc ttccgatctt
     1141 ttcgcctacg ggggtatctg aaacgaaaaa atagaggttg atcggatcaa aggttccggc
     1201 atcgagttta tcaaaatgtt tgatcaggct gaccgtcgag ttgatttcgg agccgtatac
     1261 ggtggcggtt tcgggatccc cccaacttct cgataacatt tgttgcacga tgggctcgac
     1321 aacgccctta gtgaagtcct cattgaaatc cgggagtttg gagatatttc ccgcaagact
     1381 cgcgcctacg gttgcgatga gggttgtgta ggttttcaag tttacctcct tgttattcgg
     1441 gattcgatga aggttgttcg tagaactcca cttcgagatg ctcgatgcct tggagtttgc
     1501 aaatcccggc ttttttgaat tcttcttcaa cgtccttttt taccttgctt ttcacacttt
     1561 cgaggagggc ttgggattgg tctcctctga gtacgtagtt caagaaaagc atcttcgttt
     1621 tgctctcgtc gcctcgctta gcaagcgttt tctctttgaa aaaccgggga aagacgacct
     1681 ctatggcgtt gtgcgtttcg aaatggacga attgtctaat tttgaaggcg atacgcgcta
     1741 acgtcctgat gtcgtaggag atcgaaattc gcgggtttcc gttcgcggaa tagatggcgt
     1801 cggcgacggt ctcgtaaacg gttttttcca aggttttccc gatgtcatcc aaattcgtcg
     1861 agatcgacgc gtcgcaatgg tcagggttgt agagtttcaa ctggcgtttg gccgccaaga
     1921 gcttgctttt caacccattc aaggctatgc agggattagg aaacctcaac agctcttcga
     1981 tgagttcgag cgattcgtat ttcgtacgct cgcaacggat tttgctgtat tcgggaatga
     2041 gtgtgcggtc tgcttgcacg tccgtgccgt cgataaactt caaccagcgg gcaagcgcga
     2101 tcaccatcgc cctccattcg ggttttccgg ggaaaacctc ctcacatacg tccgccagag
     2161 gcggacagtg taactcgaaa atcgagacga aatctttatc caacgtcttc tctacggcgg
     2221 ccgggcggtc gataggcata tgcccgcgat gatatcgcga cactaaaacg agcgcctttt
     2281 ttatatcacc agtggggtcg aattcttcaa gcccctccag cagtcgaaaa cgttggtcct
     2341 cgtcgcttcc atcgatcatc tgtaccgtga gctcgttatg taggtcacgc acgatgttcg
     2401 gcagcccgtc caggtgcagg acttgaccgt ttccgaacct ccacaggttg ttggtgtgtc
     2461 ccagatcgtg gatgttcatc gcaatcgcca gaatgaaata gaaatccttt atatgtgtac
     2521 gcggaatccc tttgaggaaa gtctcttctc ctatggtgtt gatcaggttg accgtgaatt
     2581 ccatcagtcg cttgctatgt cgttgcgagt gttccacggt ttcggggatt tgatcgccca
     2641 tccattgtaa agaccagcga gaaaggatct tgtcgcgtat ccaggctttt ctcttttcgt
     2701 cgtcgagaaa atccaaaaat tcttctccgt atccgaaagg tacttttcgg gaatcttcat
     2761 atttcgagag gatcctatcg ataggtaatg aagtgaggat cggttctgcc tctttaggtt
     2821 ccggggcgat ggagttgaag atgttcctta gcggttgtgg gacttgaaga tactgaacgt
     2881 aagagatccg atttccattg atttgcctgt aaaaggcgtg gctttcatcg atttgtagaa
     2941 tatcccagtt gatcggagcg ggcgggaatt tgtaaagaac atcgctgtcc gcctgaacgt
     3001 aatagaatgg ttttttgctg aattggaagt acaaagcggc gataatgcca atgatcttgt
     3061 gacccccggt ggagtcaaaa acgatttctc gtcctccgtt gtttcgctcg aggccttcaa
     3121 aagcttgcca ggtttcctta aaagaccggt acacactttc gggatcgttt gcggcatagg
     3181 agtgagaata gctactcacc gtcacgttat gaaacacgcg ctgggtcaac tttttgagat
     3241 atggatagag cttcgggcat cggttttttt cgtcctcaaa cgcgccgtct ttttcgggaa
     3301 ataagaaata acgcacttca tcgatctcgt acccgttttt ttcgatcgcg aataagctgt
     3361 cgagttcggc ggacaaaccg cgaacgcatt cgtgcgcgag aggagcctcc gcttctaaat
     3421 cgaggatttc ttttatcggt atgccgtaag ttcttccttt gggtttgaac tctgtttcac
     3481 cgtatttttc gtaagggtag gtgaggtccg aaatattcgc tttcacatta ttttgcgacg
     3541 attcggcccg tatcgctttc tctatgttcg attccaacga cttcccttgc aaaaccgcta
     3601 cgatcactgg gcctttcaga cggttttgca gggttaagat gagagagtgg acggggtttt
     3661 tcacatcggg ttcccttttt ttcgcgaggt ctctgttttt ggaggtctga cggcgcgtga
     3721 tctcttcgac acaatccgat agcccgaatt cgctcagggt tttctcgaaa gtggcatcgg
     3781 ggagttccaa gcttttcagc tcaatttcga tcatcgcgga gacatcgtct gggtgaagga
     3841 attcgtcgaa aaccacctct ttttcttcgt catccgccaa aatcgtacgt gttttgatta
     3901 cgcaggggta gtcttgaagg gcttcgattc cttcgcggag ctcgagaagt ttcgggaatt
     3961 catccggggg atactgcccg gaggcaagct tttccaaatc gatcgtttct tctaactcct
     4021 cccgcaaatc cgggtcgtca gtgtcacgct tctttccgta agtccacacg tgcctgaaag
     4081 aatttctctc tctatgtatc tcgaggcgca agcggatttc ggtgttagcg ccgcattcga
     4141 gataccactg gaccaccgcg gttttcctga catttctctt taaagtctct tctttgagac
     4201 gcttctttag cgcgcttgcg ccttgtgggt tcacatccga aagcaaccga tatttctttt
     4261 ctcgttcgat gctcattctt cctcctatgg gtccgtgact tattgtatca tttttttccc
     4321 ataattagat ttaagttcac cgctcgaaaa agacgttaat acttgatcgt attcacgtat
     4381 tcaaaaaacg gtgagacaat gaacaacggt tcggcgttat caccgaaatc agtaagaaga
     4441 taggagctct tgaatcatta ttcgagccaa accacggtaa ttcctctctt tcgagtaacc
     4501 ttcgctgagc gtagaaaagc gacgtgcgaa cggacgcgcc gctcttcagg aaaccaaccg
     4561 cagcgcaacc ccctcgaccg cgtttcaccg acagtgcttt ctcttgcggg tataatatcg
     4621 aggctcaaca aaaaaaggga agagaaggag ttccgccttt tgtcattttt aagccgcgat
     4681 ggacgcagaa aaaacgtatc gcgccgtcgc atgaagaagg ggcgcttacg ccgcgatgct
     4741 cgcgcgtatt atcgtgaggt gggtggtttt tacgaccccg aaaaaaacct gccaacttaa
     4801 aagttggcgt tcccaggcac tctcagatgt gttctaatct ctcctcagtg tccgccgtgt
     4861 tctctgtgcg aaccccattt ttcccgctca ggaaaccatg ccaaactaaa gtttggcgtt
     4921 cccaggcgtt cccgggcgtt ctcagatgtg ttctaatctc tcctcagtgt ccgccgtgtt
     4981 ctctgtgcga accccatttt tcccgctcag gaaaccatgc caaactaaag tttggcgttc
     5041 ccaggcgttc ccgggcgttc tcagatgtgt tctaatctct cctctgtgcc ctccgtgttc
     5101 tcgtgccacc cccatttttc tcgctaagga aaccatgcca aactgaagtt tggcgttccc
     5161 aggcgttccc agatgtgttc taatctctcc tctgtgtcct ccgtgttctc tgtgcgaccc
     5221 tcaattttcc cgctaaggaa accatgccaa actgaagttt ggcgttccca ggcgttccca
     5281 gatgtgttct aatctctcct ctgtgtcctc cgtgttctct gtgcgaaccc cgattttccc
     5341 gctcaggaaa ccatgccaaa ctaaagtttg gcgttcccag acgttcgcag gcgttccgat
     5401 tctgtccttg tttcttgatt acacctcata acttgaccca cactgctttc tcttgttcac
     5461 gggaaagatg ttataatcat tacgttactc gattcgtatc ttgatgtttt cctatgtcgt
     5521 accgcacgct cacgttcgtt ataggcgtgc atccgggctt gaggccgtag cccttcaagt
     5581 actactacac actttagagg tcgggtgtcg aaagaactcg gcaaggagaa tggaatatgc
     5641 ctttcggaat agatttttat atgaggatga cctccggaat cacgcgcttt ctacgagaaa
     5701 acttgaaaga cccagagatc gcttggactt tggaagcagc gaaccgtttg tgtacgtccg
     5761 acacgcttca gagcgcgcga tgcgccctcg tttcgccacg gtttcttcaa gaccgcgcgc
     5821 tctcttttga acacctcgtc ctcttgcccg acgaagaagg tgtctatacc tatcggaaga
     5881 agcgttacta ttctcctttc acgcttttcg aaaagggcgt ggcttttgat gcccccgttt
     5941 ccttcaagag cgaagaagca aagcggacgc cgccgcctaa aagaatggat ttggaggagc
     6001 tttacgtaat gcccggatat tacctccacc ttgctttgtc tcttagcagg ttccttcgga
     6061 gcttttggga tgacgataaa aacacgcgca aagccattga ggaatgggta tttgagaggt
     6121 atgtggattt cctttcttcg aacctttcga cagtacctct taagaagaag tatcacccc
//
LOCUS       DAOPXN010000136         6148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12719_length_6148_cov_5.020844, whole genome shotgun sequence.
ACCESSION   DAOPXN010000136 DAOPXN010000000
VERSION     DAOPXN010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12719_length_6148_cov_5.020844"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKM49_07635"
     CDS             <1..195
                     /locus_tag="PKM49_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82715.1"
                     /translation="DEDTLEPYKTYNWGVNIAFACVKDADSRAYSIAADYKIPYYGWG
                     FDPIGCMEPDLHAAFTTGAQ"
     gene            211..2580
                     /locus_tag="PKM49_07640"
     CDS             211..2580
                     /locus_tag="PKM49_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HNY82716.1"
                     /translation="MKKLITIFSVFFIIALVFTGCIRQTGADTNPVPPEDLPEPYPFA
                     VSYTAYDGYEVLAHTLLVGYDHLESLEALARTLGAEIRRALPQINVAELQIPNDVAVT
                     LEKLKGKSIEGIRYIEPSYKRELNQPTTQRSILTQSRTTDDPFYDYLWGLKKINAEAA
                     WALGYTGEGVIVAVCDDGSDASHPDLAGQYVIGYDAVLEKEIPAGESVPYDSHGTHVS
                     GTIAAVRDNREGIVGVAPDAKIMPIPIFYGPKSEYIGDLKVLEAWIWAVDHGAKILQN
                     SWGGEGYSHTLKRGIDYALREGAIVVVSTGNSHISENWGYPNNFPGVLGVGASTVNDS
                     LTDFSSRGDSVSVVAPGERILSTIGMDDPETLQGGLPYAYYNGTSMASPHVSGLAALL
                     YQKYPDATPYEMRKLIENSAVDLDVPGYDKNTGYGRIDALAAVQQTASTRQDLGGNLK
                     VIVLCKDETFGIPFCNVSLKRRGKPSYYALSDLRGWPVNFLGIDPDTYDVYISGPSYK
                     AIGSRLEEQLTYTQKGVVVGEDTLLTVNLASTFSAEITMPEKPGQYTAKIVGKDDGDE
                     FQKQTVGPGGTVEFTKPVETEEAFFYITVEASPAPAPLVSILTESFETGDFSNADWDW
                     SLGGDALPIVTDEEAFDGTHSARFGEIDNEGVSWMRATPNIPTGSYWLKFNVKCSTEE
                     GWSFVTVSIDGALIYTCSGVFDWEEVTLPYSGGEIAFEYEKNELETERTDTAWIDNLR
                     IIPIPEDFDDYYVTGTVTLNGHLIPVSQNLYLGSEVDEYEWDTVPWLIF"
     gene            3597..5588
                     /locus_tag="PKM49_07645"
     CDS             3597..5588
                     /locus_tag="PKM49_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82717.1"
                     /translation="MKVTSYGVKLVTLISVLLIVACGAIGILSYLFARQTLLAEVDTL
                     LALEAKNAAQNIKLREERFLAESQGAANRPDIRSMDWAQQKPALIEETQLIGYLAMAI
                     VGKDGVAHYTNETTANLGDREYVIKAFQGISNVSDVILSRVTNSLVVMIASPIRDDKN
                     TISAVLIGRLDVKTLSELVLSIDLGGQGYAYMVDKKGTIIAHHNTDLVLNQVNYIEEA
                     KKDKQYEDISNMLQKMVKQETGSASYFYEGSQRYLSYTPAGLNGWSIAVGIFPEIKLK
                     PLNQLGFRLIWISLAVLALGILASFFIGRISAKPLKVMSAGIEKFGSGDLTQKFNIKG
                     KDEFSQIGEKLNWMSERLKESMAAVLDSSTQLNDASESLTQTAVKSSEDADRLSEQAA
                     VVMKETEATSTLFMEVTGAVERMAQYVQSVSHSSQELTKMASVSAKAIGEGQTALEGV
                     VSSSNQANDQTEKTAKVIGSLSEKTKNVGAIVESIGSIAEQTNLLALNAAIEAARAGD
                     VGRGFAVVADEIRKLAEESRKAAVDISRILKQIEQETGQASTSIQDAVTFADTVVQRT
                     ERAAAEFTYIREKIEEMDRMIEKVAANSAEQSGATDEIGSSIATAAKGVKEILSNMKQ
                     MVEAINRQDQYAQNVSASSEELNALSISVKEALEQFKIS"
BASE COUNT         1537 a         1607 c         1695 g         1309 t
ORIGIN      
        1 gacgaagata ccctcgaacc ctataaaaca tacaactggg gcgtcaatat cgccttcgca
       61 tgcgtcaaag acgccgacag ccgagcctat tcgatcgcgg cggactacaa aattccatat
      121 tacggttggg gcttcgatcc aataggttgc atggagcccg atctgcacgc cgccttcacg
      181 accggcgcgc aataaggggg atgaatagaa atgaaaaagc tcatcacgat attttctgtc
      241 ttttttatca tcgcgctcgt tttcaccggg tgcatacgcc agaccggcgc ggacacgaac
      301 ccggtcccgc cggaggacct gccggaaccc tatccttttg cggtcagtta caccgcctac
      361 gacggctatg aagtccttgc ccataccttg ctcgttggtt acgatcacct tgagtcattg
      421 gaagcgctcg cccgtacatt aggggccgag atccgacgcg cgctaccgca aatcaacgtc
      481 gccgaactgc aaattcccaa tgatgtcgcc gtaaccctcg aaaagctgaa aggtaagtca
      541 atcgaaggca tccggtacat cgagccgagt tacaaaagag aactgaatca gccgacgacc
      601 cagaggagca tactcaccca atcgaggacg acagacgatc cgttttacga ctacctttgg
      661 gggctgaaaa agatcaacgc ggaagccgcc tgggcattag gatacaccgg cgagggcgtc
      721 atcgtcgccg tttgcgatga cggtagcgac gcgtcgcacc ccgacctggc cggacaatac
      781 gtcatcggat acgacgcggt tttggaaaag gagatacccg cgggagagtc cgttccctac
      841 gactctcacg gaacgcacgt gtccggaacc atagccgccg tcagagacaa tcgggaaggc
      901 atcgtcggtg tcgccccaga tgcgaagatt atgccgatcc ctatcttcta cgggcccaag
      961 agcgagtaca tcggagattt gaaggtttta gaagcttgga tttgggcggt cgatcacggg
     1021 gcgaagattc tgcaaaacag ttggggcggg gagggctact cgcacacgct gaaacggggg
     1081 atcgactatg ccctacgcga aggggcgatc gtcgtggtgt ccacggggaa ttctcacatc
     1141 agcgaaaact ggggataccc aaacaacttc ccgggtgtct taggcgttgg cgccagtacg
     1201 gtcaacgatt ctctgacgga tttctcctct cgcggcgata gtgtgagcgt cgtcgcaccc
     1261 ggagaaagga tcctgtctac gatcggtatg gatgatcccg aaaccttaca aggcggtttg
     1321 ccgtacgcct attacaatgg cacctcaatg gcttctccgc acgtttccgg cctggccgct
     1381 ctcctctatc agaagtatcc cgacgcgact ccttacgaaa tgaggaagct gatcgaaaac
     1441 tccgccgtgg atttggacgt cccgggatac gacaaaaata cgggatacgg aagaatcgac
     1501 gccttggcgg cagtccaaca gaccgcctcc acacggcagg atctcggcgg taacctaaag
     1561 gtgattgttc tatgcaagga tgaaacgttc ggcatcccct tttgcaacgt ctcgctcaaa
     1621 cgaaggggga agcccagcta ctacgcccta tctgatttac gggggtggcc ggtaaacttc
     1681 ttgggtatcg acccggatac ctacgacgtg tacatttccg gccccagtta taaagctata
     1741 ggcagccggt tggaagaaca actcacctac actcagaaag gggtcgtcgt cggagaggac
     1801 accctgctca ccgtgaatct ggcgtctaca ttctctgccg aaatcacgat gccggaaaaa
     1861 ccggggcagt acaccgcgaa gatcgtcgga aaagacgacg gggatgaatt tcaaaaacag
     1921 accgtcgggc cgggcggaac cgtagaattt acaaaacccg tcgaaaccga agaggccttt
     1981 ttctatatca ccgtagaagc ctctccggcg ccggcgcctc tagtctccat cctgactgaa
     2041 agcttcgaaa ccggggattt ttcgaatgcg gattgggact ggagcctagg cggcgatgcc
     2101 ctaccgatcg tgaccgatga agaggccttc gatggaaccc actccgcgcg gttcggcgaa
     2161 atcgacaacg agggagtcag ctggatgaga gccacgccga atattccaac cggtagctac
     2221 tggttgaaat tcaacgtgaa gtgttcgacc gaagaaggat ggagcttcgt cacggtcagc
     2281 atcgacggcg cgctcattta tacttgttca ggtgtattcg attgggaaga ggtgacgctt
     2341 ccttattctg gcggagaaat cgcgtttgaa tacgagaaaa acgagctcga aacggagcgc
     2401 acagacaccg cttggatcga caacctccgt atcattccga tcccggagga tttcgacgat
     2461 tattacgtca ccggcacggt aaccctcaac ggccacttga ttccggtttc acaaaaccta
     2521 tacctgggat ccgaggtcga cgaatacgag tgggataccg ttccttggct gatattctaa
     2581 cgccttagga atttccgaaa cagtctatga aaaagcccgc cgacgccggg cttttatctt
     2641 gggtttcggg gtgtgccctg gaaccccctc gtttctgttc ggtatgtctt ttggtagaaa
     2701 gaacaagggt tcgcacggag aacacagagg ggagaccccc actgggaacc cctgggaacg
     2761 ccagacttta gtctggcata tctttttgct ttaaaaacgt atcgcgccgt cgcaaaaaga
     2821 aggggcgctt acgccgcgat gcccgtgtgg gatatcgcgc catagggtaa tcgcggggta
     2881 accgaaggga ccccgcgatc ccccttttgt tctctttaag ccgcggtgat gtcggggtat
     2941 cgcgccgtcg caaaaagaag gggcgcttac gccgcgatgc ccgtgtaggg gtatcgcgcc
     3001 atagggtaat cgcggggtaa ccgaagggac cccgcgatcc cccctgttca aaagggttat
     3061 cgcgctatcg caaaaagaag aagcgcttac gccgcgatga ccgtgtgggg tatcgcgcca
     3121 tagggtaatc gcggggtaac cgaagggacc ccgcgatccc cctgttcaaa agggttatcg
     3181 cgctgtcgcg aaaagaagaa gcgcttacgc cgcgatgtat gttttctccg cgccctctgt
     3241 gatctctgtg cgaccccctt ttttcttttt tcaaaaaaca tgccaatcta aagattggcg
     3301 ttcctaaggc gtttccagaa gttcccaggc atttccaaaa gccagaaagt ccaccgttct
     3361 ggcggcgccc tgcggcgcct gcggattgct ttagaaatag agttcaataa tttctaaaat
     3421 aaactcacag actagtgaga atgtcgccct aatatggtaa aatattgatt gtcgcctggc
     3481 tctattttcg tcgcgatgta ccccgttttc ttgtgagact gagcaggaaa aaatgctttg
     3541 gcctgaagcg cgagtttagg gtgacaaaac ctgagaatgt ttaggaggtc gtcgatatga
     3601 aagtaacgtc ttatggggtg aagctggtca cgctgatatc ggtacttttg attgtggctt
     3661 gtggcgccat cgggattttg tcctaccttt tcgcgcggca gactcttctc gcggaagtgg
     3721 acacgttgtt ggcgctcgaa gcgaaaaatg ccgctcaaaa tattaagctg cgtgaagagc
     3781 ggtttctcgc tgaatcgcag ggcgccgcga atcgccccga tatccgttcg atggactggg
     3841 cgcagcagaa gcctgcgttg atcgaagaaa cccaacttat cggttattta gcgatggcta
     3901 tcgtcgggaa agacggagtc gcccactaca cgaatgaaac aaccgccaat ttgggagatc
     3961 gcgagtacgt gatcaaagcc ttccaaggca tttcgaacgt gtcggacgtc attcttagca
     4021 gggtcacgaa ctcgctcgtc gttatgatcg cttctccgat acgtgacgat aaaaacacca
     4081 tttcggcagt tctgattggg cgactcgacg tgaaaaccct gtccgaactc gtcctctcca
     4141 tcgacctcgg agggcaagga tacgcctata tggtggataa aaagggcacg atcatcgcgc
     4201 atcataatac cgacttggtt ttaaaccagg tgaactacat cgaagaagcg aagaaagaca
     4261 agcaatacga agacatctcc aacatgcttc aaaagatggt gaagcaggag acggggtcag
     4321 cgagctactt ctatgaaggg tcgcaacggt acctcagcta cacccccgca ggtttgaacg
     4381 gttggtcgat cgcagtgggg atctttccgg agatcaaact gaaaccgctg aatcagctag
     4441 gcttccgatt gatttggatc agtttggctg ttttggccct cggcattctc gcctccttct
     4501 tcatcggcag aatttcggca aaaccgttaa aagtgatgtc ggcgggaata gaaaagttcg
     4561 ggagcggcga cctgacgcaa aagtttaata tcaaagggaa ggacgagttt tcacaaatcg
     4621 gcgagaaatt gaactggatg tcggagaggc tgaaagaatc gatggcagcg gttttggact
     4681 cctccacaca gcttaacgac gcttctgaaa gtctcactca gacagcggta aagtcgagcg
     4741 aggacgcgga ccgcctctca gagcaagccg cagtcgtgat gaaggaaacg gaagcaacct
     4801 ccacgctttt tatggaggtc acgggggcgg tcgagcggat ggcccagtac gtccaaagcg
     4861 tctctcactc gtctcaggag ttgaccaaaa tggcgagcgt gagcgccaaa gccattggtg
     4921 aaggacaaac ggcgctggag ggcgtcgtgt cttcttcgaa ccaagcgaac gaccaaacgg
     4981 aaaaaactgc gaaggtcatc ggttcgcttt cggaaaaaac gaaaaacgtc ggggcgatcg
     5041 tcgaatccat cggttcgata gctgagcaaa ccaatttgct ggcgctgaac gcggcgatcg
     5101 aggcggccag ggcaggagat gtcggcaggg ggttcgcggt ggtggccgat gagatacgga
     5161 aactcgccga agagagtcga aaagccgctg tggatatctc acgtatccta aaacaaatcg
     5221 aacaggaaac gggccaggcc agcacgtcga tccaagacgc cgttaccttc gcggacaccg
     5281 ttgttcagcg aaccgagcgc gcggcggctg aattcacata catcagagaa aaaatcgaag
     5341 agatggaccg gatgatcgag aaagtcgcgg cgaactcggc agagcagagc ggagcgacag
     5401 atgagatagg cagttccatc gcgacagccg ccaaaggcgt gaaagagatt ttgagcaata
     5461 tgaaacagat ggtggaagcg atcaaccggc aagatcagta cgcgcaaaac gtgagcgctt
     5521 ccagcgagga gctcaacgcg ttgtccattt ccgtgaaaga ggcgttggaa cagtttaaga
     5581 tatcgtaaat ctatctcttc tttacgcggc acgtctgaaa acgtcgaact tttgctcggc
     5641 acgtgttcga tgttttctcc gtgccctctg tgccctccgt gcgaaccctg tttttcaaga
     5701 tttgtacaaa agcgtatcgc gccgtcgcga aaagaagaag cgcttacgcc gcgatgactt
     5761 cccaaaaacc taagacatgc caacttaaaa gttagcgttc ctagggtcat cccaaagaga
     5821 caggccgact ggaagtcggc cccccgagct ttcttcagtc ttttctctgt gttctctgtg
     5881 ccctccgtgc gaaccctctt tttcaagatt tgtacaaatg cgtatcgcgc cctagggtaa
     5941 tcgcggggta accgaaggga ccccgcgatc cccctgtaca aatgcatatc gcgccgtcgc
     6001 aaaaagaagg ggcgcttacg ccgcgatgta tgttctctcg gtgccctctg tgttctccgt
     6061 gcgaaaccat cttttctttt aaaaagctgc cgaacgtagg gtcaagagaa ggcgccgtgg
     6121 agtcagagat gtaccgctct cgatttat
//
LOCUS       DAOPXN010000137         6088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_12888_length_6088_cov_5.496271, whole genome shotgun sequence.
ACCESSION   DAOPXN010000137 DAOPXN010000000
VERSION     DAOPXN010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12888_length_6088_cov_5.496271"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="PKM49_07650"
     CDS             complement(<1..1023)
                     /locus_tag="PKM49_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY82718.1"
                     /translation="MRNVLIAAFALSVLLNGFLLLLWIHSKRQASRRVMKEEDLAAPI
                     AAMKTEILSLRTENEGLSVRLKNLSGLVSLVSKSDLVGKDAFFHTIMTEILKVFPEAD
                     CGFIAKIDRQDWAFLASSGYDFERLKPFRYRREYFGHFPTLTELDNATDAWVDRVPPY
                     LKGIFSAACPAVSKSLTMDLEVGGQVIGNFRIDIRKNKAHSFSERTKQDFQNYCRILA
                     SLMTTHKYFTMQGRFQKDIVYSMVKILEIYDSYTRGHSEQVTLLAQQMGKALELPVSD
                     LETLYWAGWVHDIGKVLIPASILNKPGKLTLDEYEMIKKHPVWGYQVLNTSEELQEIA
                     FIVFQDA"
     gene            1160..3253
                     /locus_tag="PKM49_07655"
                     /pseudo
     CDS             1160..3253
                     /locus_tag="PKM49_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
     gene            3342..4625
                     /locus_tag="PKM49_07660"
     CDS             3342..4625
                     /locus_tag="PKM49_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNY82719.1"
                     /translation="MQNKVSESRKKRCLSDNLFSQRMDGVRSVCIGIAIKAGSSYEPA
                     RKPGIAHFTEHMVFKGTKSRTAYELKEPIEKSGGTINAFTGKESTIYFARVPDYLARD
                     AINILKSLCFEAEFPESQFHMEKQVVLEEIHSTEDDPVEQAYEGFFQEVWRECPYGKP
                     ILGNEKSINGLTREDLFDFYDSKYVCENMVFSLAGNYNPHLIDSLEGFRHGRKNRKEP
                     EIIYSPRSALIIKEKEDLQQIHLLMGIEAPSRTDGDYEALQVLNTVLSGGMSSRLFHE
                     IREKKGLVYAIDTGLVAYPLGGVFYLYSATTGEKIIPLLDELKRQLASLVEIGITEAE
                     LAYGKERLKGKLLLSTESTYATMMRNLDTGIAFGQPLSVDEMEKRIDGITAEGIRNAL
                     QKYFTSSWVVSMVIPAKSSEMIKKTELHMNGGFFL"
     gene            4655..5809
                     /locus_tag="PKM49_07665"
     CDS             4655..5809
                     /locus_tag="PKM49_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNY82720.1"
                     /translation="MKTRWTRIDEVRTGSVNSEDIFDPYSFVLLVGKGTVIDSKTLQF
                     LKKRNVTWVPVEEESDEIEKDLTLEAESAIAQSSQQMLVPDLPLILSDEKYHLSMDRF
                     HDFASVILETGVANLTDIEDLSNDIVAEVIKTEHSILNFMTDIAPGFVVKHGINCAIL
                     AAAIGQRIKQPWHYLVQIVKVALLHDIGLIHATKEKSLFYVENVLKQFAVTPIDRVRA
                     HPFLAVKLIERMNPTFLEPDVSRGIVHHHERFDGFGFPLGIQGKNINFFSRVLAVVDA
                     YDTLITKVDGKSILDPYHALKWIIHNVKGIFDPDIVRAFIDISGLYPTGTIVRLNDGR
                     IGSVISRGDRTIARPILSIEGKEVETENESGSYIVEVMGIDKMEKSRRVE"
     gene            5775..>6088
                     /locus_tag="PKM49_07670"
     CDS             5775..>6088
                     /locus_tag="PKM49_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82721.1"
                     /translation="MIKWKKVDELNRGDIIVESIRGDAASDVIVKYGDAVDEDLIHKL
                     QIRGIQWVSVHLAPETPSDITPLFSEEKIDHLESQINDVYEQIVLENRIDLEKTREIS
                     KE"
BASE COUNT         1597 a         1486 c         1663 g         1342 t
ORIGIN      
        1 cgcatcctga aagacgataa acgcgatctc ctgcaactct tccgaggtgt tgagaacctg
       61 atatccccac accgggtgtt tcttaatcat ctcgtactca tccagagtca gctttccagg
      121 cttgttgaga atgctcgcag ggatgagaac cttcccgata tcgtgcaccc aacctgccca
      181 gtacaacgtt tcaagatcgg acacaggcag ttccagcgcc tttcccatct gttgggccag
      241 caaagtgacc tgctccgaat ggcctctcgt gtaactgtcg tatatctcca aaattttgac
      301 catagaatag acgatgtcct tttggaagcg gccctgcatc gtaaagtact tgtgcgtggt
      361 catcagcgac gcgagtatcc gacaatagtt ttggaagtct tgcttggtcc tttcggaaaa
      421 ggagtgtgct ttgttttttc ggatatcgat acggaaattg ccaatcacct gaccgcctac
      481 ctccaaatcc atcgtcaagc tcttggacac cgcgggacag gcagccgaga atattccttt
      541 caagtagggt ggaacacggt cgacccacgc gtccgtagcg ttgtccagtt ccgtaagcgt
      601 cgggaaatgg ccgaaatact cccgtcgata ccgaaaaggc ttcagccttt cgaaatcgta
      661 cccactactg gcgagaaacg cccaatcctg gcggtcgatt ttcgcgataa acccgcaatc
      721 cgcttcagga aataccttca atatttcggt cataatggtg tggaaaaacg cgtccttacc
      781 caccaggtcg ctttttgaaa ccaagctgac caagcccgaa aggtttttca atcgaacgga
      841 caagccttcg ttttcggtgc gaaggctaag gatttcggtt ttcatcgctg cgattggggc
      901 ggcgaggtcc tcctctttca tcacccgacg agacgcttgt ctcttcgaat ggatccacag
      961 aagaagtaga aagccgttca gcaaaacgga tagcgcaaag gccgcaatca gcacatttct
     1021 catctcttcc aatataatct tgggagctgt tttccagcga attatagcag acgatctttt
     1081 cttagatgta ccaaaacacg aatttcattc ttaacggtta tggtagaata aggcataaga
     1141 cgaagaagga gagaaaagaa tggattacag gacttgggac agagaactgt tcggaaaaca
     1201 tttccgtgtg gaactgggga ggatggccaa gcaatccgcc ggttccgcct acgtgcaaat
     1261 gggcgacacg gtggttctgg tggtggcgaa cgtatcgacg gaaggaaaag tcggagactt
     1321 cctgccctta acggtcgaat ttcaagaaaa attttacgcg gccgggaaga ttcccggagg
     1381 atttttaaag cgggaaggga gaacgggaga gacctcgatc ctttcttcca gactgatgga
     1441 cagaccgatt cggccgttgt ttccggatgg gtttttcaac gaagtgcaga tcatagcgaa
     1501 cgtattctct ttagacaatg attcgccgcc ggacgttttg ggcatcctgg gcgaatcgat
     1561 cgcgctcaac acttcggtta tccctttcca aggcatcgtt gccggtgttc gcgtcggtta
     1621 tctgcaaaac gcgcccgttc tctttcccac ggaagaacag cttgcggata gcttgatcga
     1681 tctagtggtc gcggggacaa aagacgccat tttgatggtg gagggagagg ctaaggaagt
     1741 cgacgagtcg atcttgctcg aagccttaca agcagcgcac gaagcgattg ggcggctatg
     1801 cgattttcaa gccgacatct ttgccgagct cgatttgccc gaaaaaatga gcttcgccgt
     1861 ctccagcctt tcggaagaag aagcgagccg gctgatcgat ctgatcgacc cggcggcgct
     1921 agaagaaagg ctgttcctgt tcgggaaaaa agagcgagag aacgcattgg cggagtacaa
     1981 gaaagaggta ctgcaggcgt ttgcggaacc ccttccggaa gaggagcgag agtcgaaaac
     2041 acggttagcc gaggcatttt atgaagagag gcttcgcaag atgatgcgcc gatttgtcat
     2101 cgaaaagcgc cggcgggtcg atggcagaac ccccgaaggc atacgcccga tacgatgcga
     2161 gacaggcatt ctccctagag cccacggctc cgccctcttc acgcgtggcg aaacccagag
     2221 cttagggatc gtcacgttgg gtggtcccga tgacagtcag atgatcgaca cgatctatca
     2281 gcagggtgac aagacgttta tgctgcacta caactttccc gcgttttctg tcggagagat
     2341 caaaccctta agggggcttt cgaggagaga agtcgggcat ggccaccttg cggagaggtc
     2401 ggtaaagcac atcattcctc ctctagacga ctttccctac accgttcgag tcgtttctga
     2461 aatcctggaa tccaacggct cctcgtcgat ggcctcgatc tgctctgcct cgctcgcgct
     2521 catgcacgct ggagtcccgg tgaagaagca cgtggcgggc gtggcgatgg gtatggtctt
     2581 tgaggaagac ggcgtggagg tcttgagcga cattaacgga atggaagacc atctcggaga
     2641 tatggacttc aaggtggcgg ggacaaaaga cggcgtcacc gggttccaaa tggacatcaa
     2701 agtcggcggc atctcgcagg agattatgaa acaagcgcta aaccaagcca aagtggctcg
     2761 acttcatatc ctgaacctga tgagcgcgga gatttccgcg ccgaaacctg aaatatcccc
     2821 gtacgccccg atgatcctct cgatcgacat accgacggaa tcgatcggag agctcatcgg
     2881 cccgggtggg aagaccatca agcggttaac caaagacttc gaagtggaca tcgacgtgga
     2941 cgacttaacc ggaaaggtca gcatctgtgg gatcgatcgc gacaaaacga acctggctta
     3001 ccagtacgtc aagaatatga cgacaccgct ggtcatcggc gaaaaatacg acggcataat
     3061 cacacgggta gaaaagtacg gcgtattcgt agaaatcgcg ccgggaaagg tcggtttgct
     3121 gcacacgtca aatatgggag agaacgttcg ggacgccaca accgtgatga agatcggaga
     3181 cgcggtgcaa gtggtgatag ggaagatcga acccaccggc aagctcgatt tgaaacggat
     3241 catcgatgga aaagtagcgg cgagcacgag aaccggtcct cctcatcgcc ccgcaagaaa
     3301 acccccgtat cagagaagac gttcgtccgg cgggggtata gatgcagaat aaggtttctg
     3361 aaagcaggaa aaaacggtgc ctttcagaca atctgttctc gcaacggatg gatggcgttc
     3421 ggtcggtttg catcgggatt gcgatcaaag ccgggtcttc gtacgaaccg gccagaaaac
     3481 ctgggatcgc ccatttcacc gagcatatgg tcttcaaagg caccaagtcg cgcaccgcat
     3541 atgagttgaa agagccgatc gaaaagtccg gaggaaccat caacgccttc acaggtaaag
     3601 agagcacgat ctatttcgct cgtgtgcccg attacctggc tcgagacgcg attaacatcc
     3661 tgaagtcgct ctgtttcgaa gccgagtttc cggaaagcca atttcatatg gaaaaacagg
     3721 tggtgctaga agagatccat tccaccgaag acgaccctgt cgaacaagcg tacgagggct
     3781 ttttccaaga agtgtggcgg gaatgccctt atggcaaacc gatattgggc aacgaaaaat
     3841 cgatcaatgg gctcacgaga gaagaccttt tcgattttta cgattcaaaa tacgtatgcg
     3901 aaaacatggt cttttcgttg gccgggaatt ataaccctca tctcatcgac tccctggagg
     3961 gattccggca cgggcgaaag aacagaaaag aaccggagat catctattcg ccgcgcagcg
     4021 cgctcatcat caaagaaaag gaggatttgc agcaaattca tctgttaatg ggaatcgaag
     4081 cgccaagtcg aacagatggc gattacgaag ccctgcaagt cctaaacacg gtcctgagcg
     4141 gcgggatgag ttcgcggctc tttcacgaga tacgggaaaa gaaagggttg gtctacgcga
     4201 tcgacacggg cttggttgcc tatcccctgg gcggggtgtt ttacttgtat tcggcaacga
     4261 cgggcgaaaa gatcatcccg ctcttggacg aactgaaacg gcagctggct tcgctggtcg
     4321 aaatcgggat caccgaggca gaactcgctt acggaaaaga acggttgaaa gggaagttgc
     4381 tgttatccac cgaaagcacc tacgcgacaa tgatgcggaa cttggatacc ggcatcgctt
     4441 tcggtcaacc gttgtcggtc gatgaaatgg aaaaaaggat cgacgggatt acggcagagg
     4501 ggatccgcaa cgcgttgcaa aaatacttca cctcttcttg ggtggtcagc atggtgatcc
     4561 cggcgaaatc gtcggagatg ataaagaaaa cggagcttca tatgaatggg gggtttttct
     4621 tgtagccctg gcaagcggtg aaaggagagc gattgtgaaa actcggtgga cacgtatcga
     4681 tgaggtgcgc acgggaagtg tgaactcgga ggatatcttc gatccgtata gctttgtgtt
     4741 actagttggt aaagggaccg tcatagattc taagacacta cagttcctca aaaaacgtaa
     4801 tgtaacctgg gtgcctgtgg aagaagaaag cgatgagata gaaaaagatc tcaccctgga
     4861 agccgagagc gcgatcgccc aaagtagcca acagatgttg gtcccggact tgccgctcat
     4921 cctatccgat gaaaaatacc atctgtctat ggaccgtttc cacgatttcg cgtcggtcat
     4981 cctggaaacg ggagtcgcca acctaacgga tatcgaggac ctatccaacg atatcgtcgc
     5041 ggaggtcata aaaaccgaac attctattct gaattttatg accgacatcg cgcccggatt
     5101 cgtggtcaag cacggtatca attgcgccat cttggccgcg gcgatcggcc aacgcatcaa
     5161 gcagccttgg cattacctgg tacagatcgt taaggtggcg cttcttcacg atatcgggct
     5221 catccatgcg acgaaagaaa agtcactatt ctacgtcgag aatgttttga agcagttcgc
     5281 ggtaacccct attgaccggg tgcgggcgca tcccttcctg gcggtaaaac tgatcgagcg
     5341 tatgaatccg acgtttcttg agccggatgt ttcgcgcggg atcgtgcatc atcacgagcg
     5401 ttttgacggg tttggctttc cgttgggtat acagggtaag aacatcaatt ttttctcgcg
     5461 cgttctagcc gtcgtcgatg cgtatgatac gctcatcacg aaggtggatg ggaaatccat
     5521 tttggatccc tatcacgcgc ttaagtggat tatccacaat gtgaaaggca tcttcgaccc
     5581 ggatatcgtg agggctttca tcgatatatc cgggttatac ccgaccggga cgatcgtccg
     5641 tttgaacgac ggacgaatcg gttcggtcat atccagggga gatcgcacta tcgcgcggcc
     5701 gatcctcagc atagaaggga aagaagtgga aacggagaac gaatccggtt cctatatcgt
     5761 ggaggttatg ggtattgata aaatggaaaa aagtcgacga gttgaataga ggggacatca
     5821 tcgtcgagag catcagggga gacgccgctt ccgatgtcat tgtaaaatac ggagacgctg
     5881 tcgatgagga tttgatccat aagcttcaga tacgcggaat ccaatgggtc tccgtccacc
     5941 ttgctcccga aacgccttcg gatatcaccc ccctgttctc cgaagaaaag atagaccact
     6001 tggaatcgca aatcaacgac gtttacgagc agatcgtctt agaaaaccgt atcgatttag
     6061 agaagacacg tgaaatcagc aaagagat
//
LOCUS       DAOPXN010000138         6034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13043_length_6034_cov_5.417796, whole genome shotgun sequence.
ACCESSION   DAOPXN010000138 DAOPXN010000000
VERSION     DAOPXN010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13043_length_6034_cov_5.417796"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            597..1988
                     /locus_tag="PKM49_07675"
     CDS             597..1988
                     /locus_tag="PKM49_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY82722.1"
                     /translation="MDPNERAKMLGEGKILSLIWKFSIPAIVGMLTNALYNVVDRVFL
                     GRGVGSLALAGITVCFPIQTVMMAFSMLIGVGSATLISIRLGQGKKEEASRILGQAVL
                     FMIIIFGVFTVVGLLLLDKMLFLFGATETNLPYAREYMSVILLGSMFYAFGMGLNFSI
                     RAEGNPRIAMATMLIGGVLNIILDPIFIYVLNKGIWGAAVATVISQMVSAIWVLTYFT
                     RGQGVIRLQWRHLRVEWRLARQVFTNGSASFALQLVNSLMNVILNQSLKTYGREYSGG
                     DTAISAMGIIQSVFTFVLMPIFGLNHGLQPIFGYNYGARKIDRVFQTLKYGYLFATCI
                     CTASFLVLRFFPAPIIQIFNKTDTELLEMTVHAAFFYSLMIPVIGVQIVSVGFFQALG
                     KPKSAMFLSLSRQILFLIPLLLLLPRFFKLDGIWYSFPICDALSFVVAVAFVTRETKK
                     LRGLSTRQVEGNL"
     gene            1985..2725
                     /locus_tag="PKM49_07680"
     CDS             1985..2725
                     /locus_tag="PKM49_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNY82723.1"
                     /translation="MNLALYGIVLSTVAGLTTALGALPFFFFRKRTDERVIDALLGFA
                     SGVMLAASAFSLVQPSIEAGGLWRFLVGFGLGALAVDLIDHFAPHEHFLKGYEGPDRK
                     KVSRIWLFVMAITIHNFPEGMAVGVGAFTKDALAIAVAIGAQNIPEGAAVAAALINAK
                     YSPGKAFLVTLLTGVVEVVGGLLGVTIISFSQALLPYMMAFAAGAMIFVIGDEVIPES
                     HLNGHQRLATYSLIIGFFIMSALDVALG"
     gene            complement(2738..4465)
                     /locus_tag="PKM49_07685"
     CDS             complement(2738..4465)
                     /locus_tag="PKM49_07685"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNY82724.1"
                     /translation="MKKALRVAVSQLNTITGALNENVKNLEQGIALGHEIGADALIFP
                     ELCLNGLNCQGLSKNRTFLRETRRTIDEIIQFSKGKQPLIVFGFLEAEEDCHYSSAGV
                     IQNGKWLGSYRKIVIQPENDGVDTAFFSSGEEGIVLNIEGIKAGIVIGNDLWDRYDLI
                     SSYDRYGVCVLFHLSAFTFLSGEYAESEQRLFSRSKDFEMGIVHANAIGGQDQWIYSG
                     NSMIFDGHSRIKARGKMLEEDFVVADIELPLRGRKAETRATRCGLRFQEWPVKRIDCD
                     FSASFKPEINSKIYDYPEDESAAICAALQLSLRDYVGKNRFEKVVLGLSGGIDSAVVA
                     ALSVASLGRERVVGVIMPSKYTTEASVKDAEELANALQIKTITLPIDDLMDSYGDVLG
                     TEVELKAGSLCEQNLQPRIRANLLLALSNHNGWLVLTTGNKSEWACGYTTLFGDSAGG
                     FAPLKDLYKHQVYALGRTLNQALGTPAITENHFSKVPTAELYPGQTDQDRLPPYEEVD
                     PIVRLMVEEGYSADEVVKEGFREESVRKVWKMYLRSEFKRRVSPVGPKLTKVSFNGEQ
                     GLPITQQWV"
     gene            complement(4496..5314)
                     /locus_tag="PKM49_07690"
     CDS             complement(4496..5314)
                     /locus_tag="PKM49_07690"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNY82725.1"
                     /translation="MKTFDLNSRIGGVKGFAYPAPSPKGVIVAVHGFGEHSGRYTDFF
                     KEMNHAGFNVYTADLPGHGKTPGKRGHATIPDMVDVIEQVTSFAQKETPGVSIGLFGH
                     SMGGLVSIRTLQKNPSRYSRAAISAPLLGLPPEQEKRIKAMAWLAKLLPSLTVKSALE
                     TPWLSRNQAIVDAYIKDTLVHDKVSLALGYSFYTEIRQAFSQTDTIKLPVLFLTGSSD
                     RIVAPQASKQFYERIPSPEKAYQIYEGAYHETFYDPEHGDRFRTDIIDWFTKGL"
     gene            complement(5301..5828)
                     /locus_tag="PKM49_07695"
     CDS             complement(5301..5828)
                     /locus_tag="PKM49_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82726.1"
                     /translation="MYRIASILLSALLLFLLGFVFVHPPATVWPVFVAVPLALVALLL
                     SRDDGKRLLITLNLLFMLLILVLFTLNNKWDQASIALFLNCLSISLVFASAELLIFLK
                     IKRRPFVDLLFLIGFWSPIAIPVFFIGQRTFGLPGGILLLAALFMIVVREMFKRKKAL
                     DLYQESKGGDRDENL"
BASE COUNT         1334 a         1567 c         1599 g         1534 t
ORIGIN      
        1 agcatcgcgg cgtaagcgcc ctttcttttc gcgacggcgc gatacgcttt tgtacgtgtc
       61 ttgaaaaaca gggttcacac ggagggcaca aagaacacag agaaaagact gaagaaagct
      121 cgtggggccg acttccagtc ggcctatctc tttgagaacg ccttgggagc gccaacttta
      181 aagtcagcct gtcttaaaag cgaaagccag gagagagcgc accgctggat ttcgttagta
      241 tcgcgagact tcgtgggttc cgaagtcagc ccgctggtta ccccgcgatg aacctctttg
      301 ttatgtcacc ccataacttg acccacacct tgcgttcatc gaaggcgtct tttggggtat
      361 aatatcaaaa ggcgttcatc ggtacggttg tccaacgtcc ccttgtgaag ctccttaacg
      421 gtcgtagacc cggtttttgt tctgtatccg aagcgcaagg tgggtttttt cgatagggac
      481 gcccttcgtt cttttttccc gtgagttggg taggcgacga cccagcgacg gttggcgctc
      541 gtagaaaaac tatgtagtcc aaaggattct agagtcatca gataggagtg cgaaacatgg
      601 atccaaacga aagagccaag atgctcggag agggaaaaat cctctcgctc atctggaagt
      661 tcagtattcc cgcgatcgtg gggatgctca ccaacgcttt gtataatgtt gtggatcgcg
      721 tctttttggg gaggggcgtc ggttctttgg ccttagccgg catcacggtg tgtttcccca
      781 ttcagacggt gatgatggct ttttctatgc tcataggcgt ggggtcggcg acgctgattt
      841 cgatacggtt gggccaaggt aagaaagaag aagcttcacg gatattgggg caagccgtct
      901 tgttcatgat cattatcttt ggtgtgttta cggtcgtcgg gctattgctg ttggataaga
      961 tgctctttct cttcggtgcg accgaaacga acctgcctta tgcaagggaa tacatgtcag
     1021 tcatcctcct cggatcgatg ttctacgctt ttgggatggg attgaacttc agtatccggg
     1081 cggaaggcaa tccgcggatc gcgatggcga cgatgttgat aggcggcgtc ttgaacatca
     1141 tcctcgatcc gatcttcatc tatgtgctca acaaaggtat atggggcgcg gcggttgcga
     1201 cggtcatctc tcaaatggtc tcggccatct gggtgctgac gtacttcacg agagggcagg
     1261 gcgtcattcg tttgcaatgg cgtcaccttc gcgtggaatg gcgattggct aggcaggttt
     1321 tcaccaatgg ttcggcttct ttcgcgctgc aactcgtcaa cagcttgatg aacgtgattt
     1381 tgaaccagag ccttaaaaca tacggtagag aatacagtgg tggagatacc gcgatcagcg
     1441 cgatgggaat catccaaagc gtcttcacct tcgttttgat gccgattttt gggctcaatc
     1501 acggattgca gccgattttc ggatataact acggagcgag aaagatcgat cgcgtattcc
     1561 agacgctcaa atatggctac ttgttcgcaa cctgcatatg caccgccagt ttccttgtcc
     1621 tcagattctt tcccgccccg atcatacaaa tcttcaataa aacggacacg gagttacttg
     1681 aaatgacggt tcacgcggca ttcttttatt cgttgatgat accggttatc ggggtgcaaa
     1741 tcgtttcagt cggttttttt caagcgctcg gaaaacccaa gagcgcgatg ttcttgagtt
     1801 tgagtaggca gatcctcttc ttgattccgc tgttgctact cttacctcga ttttttaaat
     1861 tagatggcat ctggtacagc tttcccatct gcgacgccct gtcgtttgtg gtggctgtcg
     1921 cctttgtcac gcgagaaaca aaaaagctac gaggactttc gactagacaa gtggagggga
     1981 acctatgaat ttggccctat acgggatcgt cttaagcacg gtagcgggat taaccaccgc
     2041 cttgggcgcg ttaccctttt tcttcttccg taagcgcact gacgaacgcg tgatcgacgc
     2101 cttgctcggc ttcgcgtccg gagtcatgtt ggccgccagc gccttcagct tggttcagcc
     2161 ttctatagaa gccggaggtc tctggcgttt tttggttgga ttcggtctgg gagcccttgc
     2221 ggtggacctt atcgaccact tcgctcctca cgagcacttt ctgaaaggtt acgaaggtcc
     2281 ggatcggaag aaggtgtcgc gcatctggct ctttgtgatg gcgatcacga tccataactt
     2341 cccggagggg atggctgtgg gtgtcggcgc tttcaccaaa gacgcgttgg cgatcgccgt
     2401 agccataggc gcgcaaaata tcccagaggg cgccgctgta gccgccgcgc tgatcaacgc
     2461 caaatattcg cccggaaaag cctttctggt cacgctcttg accggggtcg tcgaagtggt
     2521 cggcggactt ctcggcgtga cgattatttc tttcagccaa gctttgttgc cctatatgat
     2581 ggccttcgcg gccggcgcga tgatcttcgt gatcggtgac gaggtgattc cggaaagcca
     2641 tttgaacggc caccaacgct tggcgaccta ttcgctcatt atcgggtttt tcataatgtc
     2701 cgcgcttgac gtggccttgg gatgatcata ccgccagtta cacccactgt tgagtgatcg
     2761 ggagaccttg ctctccgttg aaactcactt ttgtcaactt cggcccaacc gggctcacgc
     2821 ggcgtttaaa ctcactgcga aggtacatct tccagacctt tcgcacactt tcctcgcgga
     2881 acccttcttt cacgacctca tcggccgaat agccttcttc taccatcaag cgaacgatcg
     2941 ggtccacctc ctcatatgga ggtaaccggt cttggtcggt ctgcccgggg tatagttcag
     3001 ccgtcgggac cttgctgaaa tggttctccg tgatagccgg ggttccgagg gcttggttga
     3061 gggttctacc caacgcgtag acctgatgct tgtacaggtc ctttaacggg gcgaatcctc
     3121 ctgcggaatc gccgaagagc gtcgtgtatc cacacgccca ctcgcttttg ttgccggtcg
     3181 tcaagacgag ccagccgtta tggttcgata aggccagtaa caggttcgca cggattcgag
     3241 gctgcaagtt ttgctcgcat aagctcccag ctttgagttc gacttcagta cccaacacgt
     3301 ccccgtagct atccataaga tcgtcaatgg gtagtgtgat cgtcttgatc tgaagagcgt
     3361 tcgccagttc ttccgcgtcc ttgacgctgg cttcagtggt atatttcgag ggcataataa
     3421 cgccgaccac ccgttcgcgg cctaagctcg cgacggacaa ggccgcgacg actgctgaat
     3481 ctataccgcc gctcaatccc aagaccactt tctcgaagcg atttttcccg acatagtctc
     3541 gtaaggaaag ctggagcgcg gcgcagatcg cagcggattc gtcttcggga tagtcgtaga
     3601 tcttggaatt gatctccggc ttgaacgagg cggagaagtc acagtcgata cgtttgacag
     3661 gccattcctg aaatcggagc ccgcaccgcg tcgccctcgt ttcagccttt ctaccgcgta
     3721 ggggtagttc gatgtcagcg acgacgaagt cttcctccaa catcttgccg cgcgctttaa
     3781 tccggctgtg gccgtcgaag atcatgctgt tgccagaata gatccattga tcttggcctc
     3841 cgatcgcgtt cgcgtggacg atgcccattt cgaagtcttt ggaacggcta aacaaccgct
     3901 gctcggactc cgcgtactcg ccgctgagga aggtgaacgc agaaaggtgg aacaaaacgc
     3961 agactccgta acgatcataa gatgagatca gatcgtaacg atcccaaagg tcgttgccga
     4021 tgacgatccc cgccttgatc ccttcgatgt tgagcacgat cccttcttct ccggaagaga
     4081 aaaaggccgt atcgacaccg tcgttctcgg gttgtatcac gatttttcgg taggaaccca
     4141 gccatttccc gttttgtatg actccggcgc tgctgtaatg acagtcctct tccgcctcca
     4201 aaaatccaaa aacgattaag ggctgcttcc ctttcgagaa ctggatgatt tcatcgatag
     4261 tccggcgcgt ctctctgaga aaggttcggt ttttcgacag cccttgacag ttcaatccgt
     4321 taagacacag ttcggggaag atcaaggcgt ctgccccgat ctcgtggccg agcgcgatcc
     4381 cttgctccaa attcttcaca ttttcattca gggcgcccgt tatcgtattc aattgactga
     4441 ccgcgactct caaagctttt ttcatacaga cctcccactc cccaagtgtt ttcgtttaca
     4501 accccttcgt gaaccagtcg atgatgtcag tcctaaacct atccccgtgc tccgggtcgt
     4561 agaaggtctc gtggtaggct ccctcgtaga tttgataggc cttttcgggg ctcgggatac
     4621 gttcgtaaaa ttgtttggaa gcttgcggcg ccacgattcg gtctgacgag cccgttaaga
     4681 acaacaccgg gagcttaatt gtatccgttt gtgaaaaggc ttggcgaatc tccgtataga
     4741 agctatatcc gagtgccagg gagactttgt cgtgcaccag agtgtctttg atgtaggcgt
     4801 ctacgattgc ctgattgcgt gatagccacg gagtctcgag cgcgctcttt accgttaaag
     4861 acgggagcag cttcgccagc caggccatcg ccttgatccg cttttcttgc tctggtggca
     4921 gcccgagaag cggcgctgag atcgctgctc gactgtaacg agacgggttc ttttggagag
     4981 tgcggatcga caccaaccct cccatactgt gtccgaatag gcctattgac actcctggcg
     5041 tttctttttg cgcgaaagaa gtgacttgtt cgataacatc gaccatgtcc ggaatcgtcg
     5101 cgtggccacg ttttccgggg gtctttccat gtccgggcaa atccgcggtg tacacgttaa
     5161 acccggcgtg attcatctcc ttaaaaaaat ccgtataccg tccggagtgt tcgccaaacc
     5221 cgtgaacggc cacgattacc cctttgggtg agggagcggg atacgcaaat cccttgactc
     5281 cacctatacg agagtttaaa tcaaaggttt tcatcacgat cgcctccttt ggattcttga
     5341 tacaggtcca acgctttctt tctcttaaac atctctctga cgacaatcat aaacaaagcg
     5401 gcgagcaaga gaatacctcc gggcagtccg aaggttcgct gccctatgaa aaacacggga
     5461 atcgcgatag gcgaccaaaa accgataaga aaaagaagat cgacaaacgg gcgtcgctta
     5521 atcttcaaga atattaagag ctcggcgctt gcgaaaacca gcgagataga aaggcagttg
     5581 aggaacaagg cgatgctcgc ttgatcccat ttgttgttga gcgtgaataa aacgaggatc
     5641 aagagcataa ataagaggtt gagggttatc aggagccgct ttccatcatc ccggctcaac
     5701 aggagagcga caagggctaa cgggacggcc acgaagaccg gccaaaccgt agctggtggg
     5761 tgaacgaata caaagcccag caaaaacagc agcagggcag agagaaggat gctcgctatt
     5821 cggtacatac cgtcactccc atagtctata aatgtgaact acggcgcgat tgattctcgt
     5881 ctttcggagg ggggttctcc tccttattga gcctcaggcg cgggtgtgtc tcagataaag
     5941 cggagtatat ctgattgagg catccaggtt aacgatttcg aatgaacgcg ccataggttg
     6001 cattatacca tttacgaggc tcggaaagta ccgc
//
LOCUS       DAOPXN010000139         6025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13070_length_6025_cov_5.314573, whole genome shotgun sequence.
ACCESSION   DAOPXN010000139 DAOPXN010000000
VERSION     DAOPXN010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13070_length_6025_cov_5.314573"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            977..2263
                     /locus_tag="PKM49_07700"
     CDS             977..2263
                     /locus_tag="PKM49_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HNY82727.1"
                     /translation="MTPYELLKKLYPINRSITGKGVETTLRIIQETLNGLQILKIPSG
                     TQCFDWVVPDEWNVQTAFLENDRGERVLDFQKNNLHLVGYSIPVDTTLSLEELQEHLY
                     SLEEMPDAIPYVTSYYRKRWGFCLSDRQRKTLKPGNYRAYIDSELKPGFLTYGELVIP
                     GEEKEEVLLSTYICHPSLANDNLSGPVVLTFLSEWLLSLPKRKYTYRVVFIPETIGAI
                     GYIHKNLEQLKRRVIAGFIFTCLGDDRAWSYLPSRNGNTLSDRVAKYVLEREVPSYTA
                     YSFLERGSDERQYCSPGIDLPIASIMRSKYGTYPEYHTSLDNLDFVTAEALDESLGIY
                     KKAIRIIERNERYKVTCLCEPQLGKRGLYPTISSKQTMGSTRDLMNLIAYADGTRDLL
                     DICQIIDADFDICVDSADRLRDAGLFKPCKAREENN"
     gene            2328..3407
                     /locus_tag="PKM49_07705"
     CDS             2328..3407
                     /locus_tag="PKM49_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNY82728.1"
                     /translation="MKTPNFLAAYEKKHANLIKPFPFEASLCLDCLLGFNSKPLPNEE
                     LDLIYDNYKYINPNRGIGTTKYEGMKRLLRKALGPSEYIVDIGCSDGYLLSYLYDHGY
                     RNLEGIDPSPVPLSPGYNIKMRREYFGENTRFDKKVDSFLLIHVWEHFPTPLRVLEMM
                     RSHLDAEGKVVMEFPNFSGFCHQHLLYYNKYFLRRLCIDQDISIEHYEEERDAIRLIM
                     KFGEKKTTPDFHKSEKEAFRQSVFKIRDKSQTAKKSLISFIQSRQNSPIIWWGSGSVS
                     VIALSQIPEEILRTANITVVDSDTTRKGLFLPGFQGQIQYADETLRGSHIETLVIATS
                     FRKEILEKIASLGCTVGSEYQIEYI"
     gene            3394..5154
                     /locus_tag="PKM49_07710"
     CDS             3394..5154
                     /locus_tag="PKM49_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HNY82729.1"
                     /translation="MNTYERKYLTLLEALEIINRKKEETQREFPVFYAAGYTPLHLLS
                     FTQAYLQTSIPHERVIIKSGLFNDLIGNISMSKREEYRHLLIHIEWPDLDPRLGLRSS
                     SGWDLSMLTDVIETIEKKTHALIAEVESAAASVPITLCFPTLPIFPMSYQPPYLLQSL
                     RTKMDGLMHRVQQRFIDSSRVQMIDPYWLDLHYTGYDRYDAESEISFGFPFTLPFASF
                     LGALFADTIRVPAPKKGLITDLDGTLWSGILGDVGEEGIQWSLDHHAMHHGLYQRLLL
                     SLSETGALIAVASKNDMFNVEKAFQRQDLIFTKDKAFPIQAHWNPKSLSVREILRTWN
                     IGEDSVVFIDDSPLELEEVKRHFPKLTCLRFPKEEPKQILPFLHTLRQLFGKRQVTEE
                     DKYRVESIRTSSEVFQVDGPEATVDQEALLSSIQSKIEIYTKGLKTDTRAFELVNKTN
                     QFNLNGERYSQSAWDEMCESPNFVGVALGYKDKFGPLGKILVLLGEVEDGALDVRSLV
                     MSCRAFSRRVEHRCLQWLFENYELREMRFHFRPTEKNGPIQSFFKEYEMNTEDPLLVL
                     KRDDFYEKVPELYHAVEVKD"
     gene            5155..5400
                     /locus_tag="PKM49_07715"
     CDS             5155..5400
                     /locus_tag="PKM49_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNY82730.1"
                     /translation="MASESVERLNRCFVAVFPGIGQNEIEMASTDNTQEWDSIATVML
                     FSLISQEFGIKILPQQMFELKSYSAIHAFLTKQGKMI"
     gene            5397..>6025
                     /locus_tag="PKM49_07720"
     CDS             5397..>6025
                     /locus_tag="PKM49_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HNY82731.1"
                     /translation="MNLTRTPESISKELDRVAQALTPKALTLHTNVGMLGVLEGPFTK
                     EWQLSEYHRLIWQAFEGTSLLYPTFNYDFLKNGVYDVKNDICKVGSLNEHVRKTHLER
                     RTHTPVFNFCILGDHPHPIQTISENPFSRESVFSNIAEGNSWIGFMGAGFDTNTFVHY
                     VEEEMNIGYRYIKTFKGRVIAENTQRAVTFRYRVRPLQQGAVDYDWVRL"
BASE COUNT         1709 a         1498 c         1442 g         1376 t
ORIGIN      
        1 gcatgtctta aataattgtt cgcccagagg gcacggagaa cacagagaga acacaaagat
       61 aaaatgcccg gattaaaaac aaaagatcgg gttcgaacgt agagcacgga gaggacagac
      121 atcgcgtcgt aagcgctttt tcttttcgcg acggcgcgat accgcggttt tcgtttgtac
      181 taaagattaa aacacggctc gcacagaggg cacagaggac acagagagaa cacaaaggaa
      241 aattgccttg attaaaaaca gaagatttgg ttcgcacaga gggcacagag gactcggagg
      301 aaagtacggg tagattcaaa cgaaaacctg tttggcatag tgggaacggt gagaaaagag
      361 ttttcaagat tcattgtgta cttctctaag ctaaactttt ctcttctttc ttcgtgctct
      421 ctctgtgttc tccgtgttct ctgtgcgaaa ctttctttat tttttggttt ttaccttctc
      481 cgcgctctct gtgcgaaact ctgtttttcg agacatgtac aaaagcgtat cgcgccgtcg
      541 cggaaagaag aggcgcttac gccgcggtgc ttgtttcctc cgtgccctct gtgttctctg
      601 tgcgaaaccc tctcttttct cttgtaataa actatgccaa ctaaaaattg gcgttcccag
      661 caaccttgcc aattaaaaac aagcgttccc agaaacagcg cgaactaaag ttcggcgaac
      721 ccaagcgctt ccagtaaaac ccaccgtgcg aacccccttt cctcttttcc acatccgggt
      781 aaaacagaag acaaaacacg cttacgcatc ccgtctatca aagaatttca caacgaaatg
      841 cattacaagg tgatataata tgaaaccgcg gccaacagca aaccctgtcg tcggcccggg
      901 ttcataaagg aaaggcggcg aagggtcgat aatcaataga cgatagcgcc caaagacctg
      961 aagagcaggt gaactcatga ccccttacga actcctgaaa aaactgtatc caataaacag
     1021 aagcattacg ggcaaaggag tggagacgac gctccggatc atccaagaaa ccctgaacgg
     1081 cctacaaatc ttgaaaatcc cctcgggtac ccaatgcttc gactgggtcg ttcccgacga
     1141 atggaacgtg caaactgcct ttctcgaaaa cgatagagga gaaagggtgc tcgatttcca
     1201 gaaaaataac cttcacttgg tcggatactc gattccggtc gacaccacgc tctctctcga
     1261 agagcttcaa gagcacctct attccttgga agaaatgccc gacgcgatcc cctacgtgac
     1321 ctcatactac cggaaaagat gggggttctg cttgtcggac aggcaaagaa aaaccctaaa
     1381 acctgggaat taccgcgcct acatagacag cgagctgaaa ccgggttttc tcacttacgg
     1441 cgagctcgtg atcccgggcg aagagaaaga agaggtcctg ctctccacct atatctgcca
     1501 cccttctctg gccaacgaca acctatccgg gcccgtggtg ctcacgttct tatccgagtg
     1561 gctgctctcc ttaccgaagc ggaagtacac gtacagggtc gtcttcatcc ccgaaacgat
     1621 cggcgcgatc ggatatatcc acaagaacct cgaacagttg aaacggcgag tgatcgccgg
     1681 gttcatcttc acgtgcttgg gagacgaccg ggcgtggtcc tatttaccca gtagaaacgg
     1741 aaacacgcta tctgaccgcg tggcaaaata cgttctcgag cgcgaggttc cttcttacac
     1801 agcatactcg ttcttagagc ggggcagcga cgaaaggcag tactgcagtc ccggaatcga
     1861 tttaccgatc gcatccatta tgcgctcgaa atacggaacc tatcccgaat accatacctc
     1921 gttggacaac cttgacttcg tcacggctga agcgctcgac gaaagcctcg gcatttacaa
     1981 gaaagccatc cggatcatcg aacggaatga gaggtataag gtcacctgcc tttgtgagcc
     2041 ccagctgggc aagcgaggac tgtatccaac gatcagttca aaacagacga tgggatccac
     2101 gagggatttg atgaacctga tcgcctacgc cgacgggacc cgggatttgc tggatatctg
     2161 tcagatcatc gatgccgact tcgacatctg cgtcgacagc gccgaccgcc tgcgggatgc
     2221 gggcttgttt aaaccgtgca aagcgcgtga agagaataat tgatgaggga gggaatgtcg
     2281 tgaactgtcc ttactgttct tcaacgaaca ccgccgtgtt tctggaaatg aaaaccccga
     2341 attttttagc cgcatatgag aagaaacacg cgaatctgat caagcccttt ccgtttgagg
     2401 ccagtttgtg tttggactgc cttttagggt ttaactcaaa accccttcca aacgaagagc
     2461 ttgatttgat ttatgataat tacaaatata tcaaccccaa tcgcggcatc gggactacca
     2521 aatatgaagg gatgaagcga ttactacgga aggctttagg tccaagcgaa tacattgtcg
     2581 atattgggtg ttccgatggc tatttactct cttacctgta tgatcacgga taccgaaacc
     2641 tcgaaggtat tgatccgtcg ccggtaccat taagtcccgg ctataatata aaaatgcgcc
     2701 gtgagtactt tggcgaaaat acccgtttcg ataagaaagt cgattcattt ctccttatcc
     2761 acgtttggga acacttcccc accccgttgc gagtgctcga aatgatgaga agtcatcttg
     2821 acgcagaagg caaggttgtt atggaattcc cgaatttttc tgggttttgt catcagcatc
     2881 tgctctatta caataaatat ttcctgcgca ggctttgcat agaccaagat atctcgatag
     2941 aacactatga agaagagcgc gatgcgatca gattgattat gaaatttgga gagaaaaaaa
     3001 caacccctga ttttcataaa agtgaaaagg aagcattccg ccaaagcgtc ttcaaaatcc
     3061 gtgacaaatc gcaaacagcg aaaaaatcgt tgatctcgtt catccaatct cggcagaact
     3121 cccctatcat ctggtgggga agcggttcag tttcggtaat cgctctgagt caaatccctg
     3181 aagaaatact gagaacggca aacatcactg tggtcgactc ggatacaacc cgaaaaggat
     3241 tatttcttcc cgggttccaa ggtcaaatac aatacgctga tgaaacgctg aggggtagcc
     3301 acatagaaac attggtgatc gccacttctt tccgaaaaga aatcctcgaa aaaatcgcat
     3361 cgcttggatg cacggtgggg agtgagtatc agattgaata catatgaacg aaaatacttg
     3421 acgctactgg aggcgctcga aataatcaat cggaagaaag aggagactca aagagagttt
     3481 cccgtattct acgcggcagg ctacactccg ttgcacctcc tatcctttac ccaagcgtac
     3541 cttcaaacgt ctattccaca cgaaagagtg atcatcaaga gcggcctttt caatgatctc
     3601 ataggaaata tctcgatgtc gaaaagagaa gaatacagac atttactgat acatatcgag
     3661 tggccggatt tggatcccag gctgggatta cgttccagtt ccggatggga tctatcgatg
     3721 ctgacagacg tcatcgagac gatagaaaaa aagactcacg cgctaatcgc cgaggtcgaa
     3781 agcgcggcgg ccagcgtccc catcacgcta tgttttccaa ccctgcccat attcccgatg
     3841 tcctaccaac cgccatactt gctgcagtcg ttacgtacaa aaatggatgg actcatgcat
     3901 cgtgtccagc agaggtttat agactcctca agagtgcaaa tgatcgaccc gtattggttg
     3961 gaccttcatt atacgggata cgaccggtat gacgccgaat ccgaaatctc cttcgggttt
     4021 ccgtttacgt tgcccttcgc gtcgtttctg ggcgccctat tcgcggatac gatacgggtt
     4081 ccggctccaa aaaaaggatt gattaccgac ttggacggga cattgtggtc gggaatcctc
     4141 ggagacgtgg gcgaggaggg gattcaatgg tcacttgacc accacgcgat gcaccacggt
     4201 ctgtaccagc ggctactcct ctccttatcc gaaacgggcg ccttgatcgc cgtggcgagt
     4261 aaaaacgata tgttcaatgt ggaaaaggcc ttccaacgcc aagacctgat ctttacgaaa
     4321 gataaagcct tcccgataca agcgcattgg aatccgaaaa gcctttcggt aagagagata
     4381 ttgagaacct ggaatatagg cgaagacagc gtggttttta tcgatgacag cccgttagaa
     4441 ctggaggaag tgaaacggca tttcccgaaa ctcacctgtc tacgattccc gaaagaagaa
     4501 ccgaaacaaa tcttgccgtt tctacacaca cttcgacaac tatttggaaa acggcaagtc
     4561 accgaagagg acaaatacag ggtcgagagc atacggacct caagcgaggt atttcaagtt
     4621 gacggacccg aggcgacagt cgatcaggaa gccctcctat cgtctatcca atccaaaata
     4681 gaaatctata ccaaaggatt aaaaaccgac acgcgagcct tcgaactggt caacaaaacg
     4741 aatcagttca acctgaacgg cgaaagatac agccaatcgg cctgggatga gatgtgcgaa
     4801 tccccgaact tcgtaggcgt ggcgcttggt tacaaagaca agttcgggcc tttggggaaa
     4861 atcctggtac tcttgggaga ggtagaagat ggggcgctgg acgtccgatc cctcgtgatg
     4921 agctgcagag ccttcagccg gagagtggag caccgatgtt tgcaatggct ttttgagaac
     4981 tacgagctgc gggagatgcg cttccacttt cgccccactg agaagaacgg gccgatccaa
     5041 agctttttca aagaatatga aatgaacaca gaggatcccc ttctcgtatt aaagcgggat
     5101 gacttctacg aaaaggtacc cgaactctat catgccgtcg aggtgaaaga ctaaatggct
     5161 tccgaatcgg tagaaagact caaccggtgt tttgtggccg ttttcccggg aataggccaa
     5221 aatgaaatcg aaatggcgtc aacagacaac acccaggaat gggattcgat agcgacggtg
     5281 atgttgttca gtttgatttc ccaggaattc ggcatcaaga tcttacccca acaaatgttt
     5341 gaactcaaat cctattctgc catccacgcg tttcttacca aacaggggaa gatgatatga
     5401 acctcacgag aacgcccgaa tccatatcca aggagctaga ccgggtcgct caagccctca
     5461 ccccgaaagc attgacctta cacaccaatg tgggaatgct cggggtgttg gaagggccat
     5521 tcaccaaaga atggcagctc tcagaatacc atcgattgat ctggcaagca tttgagggaa
     5581 cgagccttct ctatccgacg tttaactacg attttcttaa aaacggcgtt tacgatgtga
     5641 agaacgatat ctgcaaagtc ggctcgttaa atgaacacgt gaggaaaaca cacctcgagc
     5701 ggcgcactca tacaccggtt ttcaacttct gtattctcgg agaccatcca caccccattc
     5761 aaaccattag cgaaaacccc ttctcccggg aatcggtctt ttcgaacata gcggagggaa
     5821 actcgtggat cgggtttatg ggggcgggat tcgacaccaa cacattcgtt cactatgtgg
     5881 aagaagaaat gaacatcgga tatcgctata taaaaacttt taagggaagg gtgatcgccg
     5941 agaacaccca acgcgctgtg acgttccgct accgtgtgag accgctgcaa caaggcgccg
     6001 tagactacga ttgggttcgg ctcga
//
LOCUS       DAOPXN010000140         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13410_length_5909_cov_4.846088, whole genome shotgun sequence.
ACCESSION   DAOPXN010000140 DAOPXN010000000
VERSION     DAOPXN010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13410_length_5909_cov_4.846088"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            375..2390
                     /locus_tag="PKM49_07725"
     CDS             375..2390
                     /locus_tag="PKM49_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82732.1"
                     /translation="MQLKSIRTKLVFALVPIMVVAAILSGLLIGRVSRSTIESLIQRQ
                     TDEKVMVMAGLIEEWHVGIQQRLQSIAYSNVIQAMRPEGFRDYLRALVENSDGLFYDL
                     FVTNTTGDSWNRAGAVENMKGRAAYQDIFTLRKPFSVSNGEADRGSGKASVSFACPIR
                     SERGEIVGMLTAVVNLERLSKKLGASPYAQDEYGFVVDGNGLAIAHSDNQSLVMKWNV
                     TKAGDSGYKDLQQIGLRMQQGETGNGEYTGPDGARYRLFYRTIPGTPHWSMGVSIPVV
                     TLQKVNQRPILFLYLTFGFLILLVAVLSYVVGLWVAIPIQAVGNELKRFGELDLRRTV
                     SKADDYLDRKDEVGQLTSALAKMVVATRSAFGSIQESVGEMNASSTQLADIAQHQTEA
                     SQELDKQVQRLESNAQNVSASIEEVSSGAQEVATTALGVSKIAMEILEESKSTSQAVQ
                     YGMQAAAEAIGSIRVAQTQTQEMAVTATEVAGQAQKVGEIVELISSISEQTNLLALNA
                     AIEAARAGEAGRGFAVVADEVRKLAEESQGATSNITAILKTLADGLRKVDESSRRTVD
                     LVKRANETGEEIDKQFSAITRNVEKINEVVGHLTVTAEEQSASADEMASATEASAEAV
                     NDIREQIERIAKSVEEQAREAGKVSLSAQQLDALARKVGDEVGKFQF"
     gene            complement(2475..2798)
                     /locus_tag="PKM49_07730"
     CDS             complement(2475..2798)
                     /locus_tag="PKM49_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82733.1"
                     /translation="MELKQPSRKAWRPHENCQVKPWHPQRAAFKHIRKNGSKGTQFKE
                     LERVSPSLSRIQLKVLVRELQQSGAAYAFGKNRTNTLVRALTWNQTWNQLGTKNAKNE
                     IAGKK"
     gene            3503..5473
                     /locus_tag="PKM49_07735"
     CDS             3503..5473
                     /locus_tag="PKM49_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HNY82734.1"
                     /translation="MKRIRIFLALICCTFGAVGAWADPMVKPATEPTKAANAAYYRLL
                     DFSDDREFENATRGVIEWTESVAVQRESGGLAWGVNAADYAGEAPGTTNPSLWRNAQL
                     NAYAGLFEVCEGIYQVRGYDMANATFIRTDTGWIVFDVMMCKENMAAALQLMEAHFGK
                     LDIKAVLYSHPHIDHFGGIEGLITKDQVADARLPLDEQLLSGKIPILAPEGFLEHAVS
                     ENVYVGKAMGRRAQYQYGVLLEQGATGRLTIGIGMGQSLGTLSLFAPTCEISERNRNI
                     WIDGLEIQFQLTPGTEAPVEMNAYFPRYRALWLAENCTGTLHNLYTLRGAQVRDAKAW
                     AFYIVEAEQLFGDKTDVVFQSHNWPHWGEEVPEYLTNTAAIYKFIHDQTLHYINLGYT
                     ATEIANTIRLPEELEKVWYTRQYYGTLSHNVKAVYQKYMGWYDANPVNLNLLSPERTA
                     QKLVEYLGDTESVLAKAREDFEAGEYQWVAQITKELVYADPKNQAARELCADALEQLG
                     YQAESGTWRNAYLTGALELRMGNQAIGAPTASGLNSTLMDMTAPMLLDYIAIITDANA
                     AQHDELSLNLIFTDTGESFYVRRINGVLLSYPGEKRENAQGTVTCAKSQLLSLLSGAP
                     VQGIKVEGDLTVLKRLVQYAQAFDGAFNIIEP"
BASE COUNT         1462 a         1536 c         1655 g         1256 t
ORIGIN      
        1 ggatgcgggg tcccgctggt taccccgcga taacacacac gggcatcgcg gcgtaaaaag
       61 ggcaaaagga gaatcatggg gtcgaaaaat ccacttaccc cgcgatgaac ctcttagcta
      121 tgtcgctcca taacttgccg cacgctaaac cggatccttt ctattccaag ccctttctaa
      181 tagatctatt ttccacagca tcctggcttc gccaaaacaa atatcgaaaa agcctaacac
      241 agccccacaa agaataccag tttgattgcc gttagcgcga gagtaccgat actcgtacgg
      301 ggttgacaac tctcatttta ttattgtata atgattgtaa actatacgga tacgactttc
      361 tgagggggtt ctttatgcag ttgaaatcca tacggacgaa attggtcttc gcgctcgttc
      421 cgattatggt cgttgccgcg attctgagcg gtttattaat aggacgcgtt tctcgatcga
      481 cgatcgaatc tctgattcaa aggcaaaccg acgaaaaagt tatggttatg gcgggtttga
      541 tcgaagagtg gcacgtgggc attcaacaac ggctgcagag tatcgcctat tcaaacgtta
      601 ttcaggcaat gagaccggag gggtttcgcg actatctgcg agcgctggta gagaacagcg
      661 acgggctgtt ttacgatctg ttcgtgacaa atactaccgg agactcgtgg aaccgggctg
      721 gcgccgtaga gaatatgaaa ggacgcgcgg cctaccaaga catttttacc ctcagaaaac
      781 ctttttccgt gagtaacggc gaggccgaca gaggaagcgg gaaagcgtcg gtttcgttcg
      841 cgtgtccgat acgaagcgaa cgtggtgaaa tcgtggggat gttgaccgcc gtcgtcaatc
      901 tcgaacgctt gagcaagaaa ttgggggcca gcccatacgc ccaagacgaa tacgggttcg
      961 tcgtggacgg taacggtttg gccatcgcgc atagcgacaa ccaaagtttg gtgatgaaat
     1021 ggaatgtgac gaaggcggga gattcggggt acaaggacct gcaacagatc ggcctccgga
     1081 tgcagcaagg cgaaaccggg aacggtgaat acacgggtcc ggatggcgcg cgataccgcc
     1141 tgttctatcg aacgattccc ggaacacctc attggtctat gggagttagc atccccgtgg
     1201 ttaccctgca aaaggtgaac caacgaccaa tacttttcct ctacctgacc ttcggcttcc
     1261 tgatccttct cgtcgcggtg ttgtcatacg tagtcggtct ttgggttgca ataccgattc
     1321 aggcggttgg caacgaattg aagcggttcg gagagctgga cctaaggcga accgtgagca
     1381 aagccgacga ctatctcgac cggaaagacg aggtcggcca gctgacgagc gcgctggcca
     1441 aaatggtggt ggcgacccga agcgctttcg gctcgattca ggagtccgtg ggcgagatga
     1501 acgcgtcctc aacccagctg gccgatatcg cgcaacacca aacggaggcg tcccaggagt
     1561 tagacaaaca ggttcaaagg ttggaatcca acgcgcaaaa cgtttccgca tcgatcgaag
     1621 aagtgtccag cggcgctcag gaggtggcga ccaccgcgct gggcgtttcg aagatcgcga
     1681 tggagatcct ggaggaatcc aagtctactt cacaagcggt gcagtatggg atgcaagcgg
     1741 cggcggaagc catcggcagc atacgcgtcg cccagacgca aacacaggag atggctgtca
     1801 ccgccacgga ggttgcgggt caagctcaaa aagtggggga gatcgtcgag ctgatctctt
     1861 ctatatctga acaaacgaac ctactcgcgc tgaacgccgc catcgaagcc gcaagggcgg
     1921 gagaagcggg tcgcgggttc gccgttgtcg ccgacgaggt gcgtaaactg gcggaagaga
     1981 gccaaggcgc gacgtcgaat atcaccgcga tcctgaaaac cttagcggac ggtctacgaa
     2041 aggtggacga atcgtcgcgg cgaaccgtcg atctggtgaa aagagcaaat gagacggggg
     2101 aagagataga caaacaattt tccgcgatta cccgaaatgt ggagaagatc aacgaggtgg
     2161 tcggccattt gaccgttacc gccgaagaac agagcgcgtc ggccgacgag atggcttcag
     2221 cgaccgaggc gtccgcagaa gccgtcaatg acatcagaga acagatcgaa cggatcgcca
     2281 agagcgtcga ggaacaggcg cgtgaagcgg gtaaggtcag tctatcagcc caacaactcg
     2341 atgccctcgc ccgaaaggtc ggcgacgagg tcggaaagtt tcaattctga cggagggatc
     2401 cgcggttttt gaatacgggt aggggtgtgg aaaaccgttc cgcaaacctt tattttagcg
     2461 tttctgacag catttcattt cttccctgcg atttcattct tggcgttttt agttccaagt
     2521 tggttccaag tttggttcca agtcagcgcg cgaaccaatg tgttggtgcg gtttttacca
     2581 aaagcgtatg ccgctccact ttgctgaagt tcacgcacca acacttttaa ttgaattcga
     2641 gaaaggcttg gcgaaactcg ctccaattcc ttaaactgcg ttcctttgct accgtttttg
     2701 cgaatatgct taaaagcagc tctttgtggg tgccacggct taacctgaca attctcgtgt
     2761 ggacgccaag ctttcctgct tggttgtttt agttccaagt aggttccaag tttggttgca
     2821 agttagttgc aagtcagtcg taagtatgaa tgcaaccgtg atttcaaatc aatgagcaaa
     2881 ccagcgtgca gccgaagtct tgcctacgac atatattagt tcatcgcgct tcaattctct
     2941 cattaaaact tgcaactgac tccgtgaatg actcggcaga acctgttcca actctttcaa
     3001 cggcgtgccc ttatcaccat ttttcgttct cttccgagtt aaacgagtag gaacacgatt
     3061 attccaacct tggtacagcg agctggattt acctggcgac caaaaagatt gccaggagcg
     3121 tctgtcaggc gataggcatc gcggtgtaag cgctttttct tttcgcgacg gcgcaatacc
     3181 ctttttagaa aggcatagtg gggattgcgg ggtcccgttg gttaccccgc gataacatac
     3241 acgggcatcg cggcgtaagc gccccttctt ttcgcgacgg cgcgataccc cttttagaaa
     3301 ggcatagtgg ggatcgcggg gtcgtaaaaa tcacttaccc cgccataacc ttctaagttc
     3361 agcgttccca gacgctgtca atgcgctgtc gaaaaatatt gacgcctcgc cttgatagga
     3421 gtatcattga tgccatctcg atggattatc cgataaacgt gcactcatac ccacaatcta
     3481 cgggaaaaaa ggagcgattt tcgtgaaaag aatcaggatt ttcttagcgt tgatctgttg
     3541 tacattcggc gccgtgggag cgtgggccga tccaatggtg aaacccgcca ccgaaccgac
     3601 gaaagccgca aatgccgcat actatcgttt gctcgatttt tccgatgatc gggagttcga
     3661 aaacgccaca cgcggagtga ttgaatggac cgaaagcgta gctgtccaaa gggagagcgg
     3721 agggcttgcc tggggggtca acgccgccga ttacgccggt gaggcgcccg gcaccacaaa
     3781 tcccagtctg tggcggaacg cccagctgaa tgcctatgct ggccttttcg aagtgtgcga
     3841 aggcatctat caagtgcggg gatatgatat ggccaatgcc acgtttatcc gcacggatac
     3901 cggttggatc gtctttgacg tgatgatgtg caaagagaat atggctgccg ccttacaact
     3961 gatggaggcg cacttcggaa aactcgacat caaggccgtt ttgtacagcc acccgcatat
     4021 cgatcacttt gggggtatcg aagggctgat cacaaaagac caggtcgccg acgcgcgact
     4081 accgttggac gagcagctcc tttccggaaa aattccgatc ctcgcgcccg aaggctttct
     4141 ggaacacgcg gtgagcgaaa atgtgtacgt gggtaaagcg atgggaaggc gcgcccaata
     4201 ccaatacgga gtgcttctgg aacagggcgc cacgggacgg ttgaccatcg gaatcggtat
     4261 gggacagtcg ctggggacac tgtcgctctt cgcacccact tgcgagatca gcgagagaaa
     4321 ccggaacata tggattgacg gtttggagat acaatttcag ctgacccccg gcacggaggc
     4381 gcccgttgaa atgaacgcct actttcccag ataccgggcg ctgtggttgg ccgagaactg
     4441 cacgggcacg ctgcacaacc tctacactct gcgcggggcg caggtacgcg acgcgaaagc
     4501 ctgggccttc tacatcgtcg aagccgaaca actgttcggt gacaaaacgg atgtcgtatt
     4561 ccaaagtcat aactggccac attggggtga ggaggtcccg gagtacctga cgaacaccgc
     4621 cgccatctac aaatttatac acgaccaaac cctccattac atcaatttgg gctacacggc
     4681 cacggagatc gccaatacga tccggcttcc ggaggagctg gaaaaggtat ggtatacccg
     4741 ccagtattac ggtactctct cccataacgt caaggcggtc taccagaagt atatggggtg
     4801 gtatgacgcg aatcccgtca acctgaacct gctgtctccg gaacgcaccg cccagaaact
     4861 ggtggagtac ctgggggata cggaatccgt gttggccaaa gcgcgggagg atttcgaagc
     4921 cggcgaatac caatgggtcg cgcagatcac caaagaattg gtttacgccg atccgaagaa
     4981 ccaagcggcg cgtgagctct gcgccgacgc cttagagcag ctgggctatc aggcggagtc
     5041 cggtacgtgg cgcaacgcct acttgacggg ggctctcgaa ctccgtatgg ggaatcaagc
     5101 gatcggcgcg cctacggcca gcgggctcaa cagcacgctg atggatatga cagccccgat
     5161 gcttctggat tatatcgcga tcatcaccga cgcaaacgcg gcccagcacg atgagttgtc
     5221 tctaaatctc atcttcaccg atacgggaga atccttttat gtccgtcgca taaacggcgt
     5281 cttgctcagc tatccgggcg aaaaacgaga gaacgcccag ggaaccgtta cctgcgcgaa
     5341 atcgcaactg ctctcgctac tgtccggcgc ccccgttcaa gggattaagg tagaaggcga
     5401 cctgacggtc ctgaagcgct tggtccaata cgcgcaagcg ttcgacggcg ctttcaatat
     5461 catcgaaccg tgaagataag tccaacctgt taggcatcta ggcgtaagcg ccccttcttt
     5521 tcgcgacggc gcgataccac ggttttcgtc cgttccaaag atcaaaaaat agagttcgca
     5581 cggagggcac agagcgcgcg aaggaataac tggcaaggca cagcgttctc gcagaaaaaa
     5641 agacatgccg gacttaagtc cggcgttccc agaaaagtgg ggatcgcgag acttcggtgg
     5701 cttccgaagt cagcccgctg gttaccccgc gataacccgc gcaggcatcg cggcgtaagc
     5761 gccccttctt ttcgcgacgg cgcgatacgc ttttgtacgt gtcttaaata acagggttcg
     5821 cacggaggac acagagaaca ctgaacccat gccacacgta gggtcaagag aaggcgccgt
     5881 ggagtcagag atgcaccgct ctcgattta
//
LOCUS       DAOPXN010000141         5776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13836_length_5776_cov_5.542562, whole genome shotgun sequence.
ACCESSION   DAOPXN010000141 DAOPXN010000000
VERSION     DAOPXN010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5776
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13836_length_5776_cov_5.542562"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PKM49_07740"
     CDS             complement(<1..167)
                     /locus_tag="PKM49_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNY82735.1"
                     /translation="MYDHYDEKREQERIINQIVPIVIETTGRYERAYDIYSRLLKDRI
                     VILGGEINDHTA"
     gene            complement(173..1579)
                     /gene="tig"
                     /locus_tag="PKM49_07745"
     CDS             complement(173..1579)
                     /gene="tig"
                     /locus_tag="PKM49_07745"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNY82736.1"
                     /translation="MQREIIEERQDIVRYRYIITLEEVAEQTEKAIRDLSARIVVPGF
                     RKGKAPLDLVRREVRAEDLLPQLNRHFIKIVADDVEKNLSGTGVIDYSFEEDFREEED
                     GTFSITMLFYRKPTVQLPDLKSITVKKLRPSEKMLDYWHEHTVKRLREDYSTLAPKDT
                     EAETGDKVAYHYQVYNSKGKQLFSGKEEIAYLIPENFNPLIEKMIGKKQGDSYSVDRT
                     LTLRDKPDEPVTYTYQVQIKEVYRLIPPELNDAFARQVSEKFNTLEDLYQNLREKDMK
                     YFDAIVEKNMKTQIENALTEMTKVDVSQETIQNKLDWYVEDLKKRGRYGDWEEDYSSE
                     EEYRESLRDELRELMTLSWAIEKVAQEHNITITDEKAKEWLRSNSKSLDNNPEKLLAK
                     YKKDKDFRDEIKLLLKNEEVMDFLLTQVTVEEEETTLDEDFNMLSEEYDEDDEGFLEG
                     DSLANLEQPDEDEPWDEE"
     gene            complement(1722..1798)
                     /locus_tag="PKM49_07750"
     tRNA            complement(1722..1798)
                     /locus_tag="PKM49_07750"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1762..1764),aa:Cys,seq:gca)
     gene            complement(2515..3657)
                     /locus_tag="PKM49_07755"
     CDS             complement(2515..3657)
                     /locus_tag="PKM49_07755"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNY82737.1"
                     /translation="MTNRSFLFFLPTKTFFGVGVTQGIGELCAQKRVMVVTTGLRRTG
                     ILNRIIREAGTPADLIVFDEIEPNPTCESVDKGGFLARVNEIEVIIALGGGSALDAAK
                     GIACLRDEREELLDASSGIRSYLSGERAFTRRHTQLIAIPTTSGTGSEVTNVAVFSDP
                     ANRLKKSIVSPWFWNDYAYVDPELTLTVPDKHTAATGLDALVHAVESYWALSSTPFSE
                     PIALEAIKLILDNLTQGYRNPEDRVARTNLSLGSLMAGIAFSQTRTTILHALSYPLTV
                     DYHLEHGFACSVLLKPMLQTNAPLIPEKMARLLDYLGSPSLDALLERIEGLMQSINAP
                     TRLSTVGVQEEELESIADQTMRSPITKLNPKSFTREELVDWLKEYR"
     gene            complement(3673..4986)
                     /locus_tag="PKM49_07760"
     CDS             complement(3673..4986)
                     /locus_tag="PKM49_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82738.1"
                     /translation="MKRACFTLLLIAVLILALTGCTSTLVYNESGAIDRFLSWLNDNG
                     YPNPPETPMVGLFLGEVKKGDVIESNSPLMESPQRAGEPLTQNGWLFYVDECPGAFYE
                     HPGRIVVVSTSGAILVNEPTNGWPTVNGNRPIQLNSATSDRYFGAIVWNPYAWCKPDT
                     HNKTWFPSAISASAKGAVVVNGCLTEETLSAEAKAISKQFLNDMKTLFGNNNVRDLTS
                     PTVMANPAEQILQAVEYLVVNRDCRNIVLYIIGHGGNDRISIGGTSYRASQLHTLINS
                     YPDVLFSVILETCHAGSWLDNFDGTTSRILENLGLFIASTSADKFAYPDWDQLTLIGI
                     FGDGLPVSVLHDHNKEDSYVEWTSDFLQQLSFWSTGAGWTAVQNYANTHGIETEWSLF
                     YHCFWKVKGAGTVPPPDIFPLGENTLTMTERMYISRQDPQIYKKW"
     gene            5375..>5776
                     /locus_tag="PKM49_07765"
     CDS             5375..>5776
                     /locus_tag="PKM49_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82739.1"
                     /translation="MKKAFGFVIAFGLCVWALSAGGLSSLFPAKVQKTPTYIGDLLIV
                     DIPPFSRVLSFEGLTDVKELKDKVIAQLALPKWSVVLFRNGLLGHRDVFGGQVAMTDS
                     DHDGFPDEAELWGDDATRFRHWFVYIAMQQKV"
BASE COUNT         1313 a         1507 c         1444 g         1512 t
ORIGIN      
        1 gccgtatgat cattaatctc gccgcccaaa atgacgattc ggtccttgag cagccgtgag
       61 tatatatcat aggcccgttc gtagcgcccc gtcgtttcga tcacgatggg cacgatctgg
      121 ttgataatgc gctcctgttc tctcttttcg tcgtagtggt cgtacatagg gttcactcct
      181 catcccaagg ctcatcctcg tctggctgtt ccaaatttgc aagtgaatcg ccttctaaaa
      241 atccttcatc gtcttcgtca tactcttcgg agagcatatt gaaatcttcg tcgagtgtag
      301 tttcttcctc ctctacggtc acctgtgtaa gaaggaagtc catcacctct tcattcttaa
      361 gaaggagctt gatctcatcg cgaaaatctt tgtctttctt gtatttcgcc aaaagctttt
      421 ctgggttgtt gtcaagcgac ttcgaatttg aacgtagcca ttctttcgcc ttttcgtcgg
      481 ttatcgtgat attgtgttct tgcgccacct tctctattgc ccacgaaagc gtcatcaact
      541 cacgcaattc gtcgcgaaga ctttcccggt actcctcttc gctagaatag tcttcttccc
      601 aatccccgta acgtcctctt tttttcaggt cttccacata ccagtcgagt ttattctgta
      661 tcgtctcttg cgaaacatcc actttagtca tttctgtgag ggcgttttct atctgcgttt
      721 tcatattttt ttccacaatg gcgtcaaagt atttcatatc cttttccctc aagttttggt
      781 aaaggtcttc gagggtgtta aacttttcgg atacctgacg cgcgaacgcg tcgttcaatt
      841 ccggcgggat caaccggtag acttctttga tctgaacctg ataggtgtag gtcacgggtt
      901 cgtccggttt atcccttagc gttaaagtcc gatctacaga gtaggaatct ccctgttttt
      961 ttccgatcat tttctctatc aatgggttga aattctcggg aatcaaataa gcgatctctt
     1021 ctttaccgga aaacagctgt ttccctttgg aattgtacac ctggtaatgg taggccacct
     1081 tatctccggt ctccgcctcg gtgtctttcg gggctaacgt agaataatct tcacgcaatc
     1141 ttttcacggt gtgctcgtgc caatagtcga gcattttttc ggaaggtcgc agctttttga
     1201 ccgttatcga ttttaaatca ggcagttgca ccgtcggttt tctatagaaa agcatcgtaa
     1261 tcgagaaggt gccgtcctct tcctcgcgaa agtcttcttc gaaggaatag tcgataactc
     1321 ctgttccgga taagtttttt tcgacgtcat cagcgactat cttgatgaaa tgcctgttta
     1381 attgcggcag gaggtcctct gctctcactt cccgcctcac gaggtccaac ggtgcttttc
     1441 cttttcgaaa ccccggaacc acgatgcgcg cactcaaatc gcgtatcgct ttctctgtct
     1501 gctccgcgac ttcttctagc gtaattatgt atcgatatct gacgatatcc tggcgttctt
     1561 caataatctc acgctgcaaa acgtttcctc cttaaaagac gagcgctgta ctttcttctt
     1621 gacgtaatta taccattatt attatcgaat actgaagcgg atcgacaatc cgatccttcc
     1681 gctagtaaaa aaaagagcga gaaggagtcc cgcccttttt ttggaggcaa cggacggatt
     1741 tgaaccgtcg aatagagatt ttgcagacct ccgccttaga ccccttggct acgttgccca
     1801 attccggcat attatatcag atgatgccca atattgcaag cgaaggtgcg cgtcaagtta
     1861 tgaggtgtta tcaagaaaca agaccagaat cgatcacacg gtttttgtcc gtgaggtcat
     1921 cgcggggtaa aaaagacaaa aggcgtatcg cgagacttcg cggactggga acgccagctt
     1981 ttaagttggc atggtttgga aaaagggttc gcacagaggg tacagagaga acagagagaa
     2041 caggcatcgc ggcgtaagcg ccctttcttc tcgcgacggc gcgataccct ggtgttcgtt
     2101 ccaaagatta aaaacagggt tcgcacagag agcacggaga acacggagga aaaccgggca
     2161 agagcgaagg tttctcggaa aagaacacgc caacttaaaa gttggcgttc ctagaacatt
     2221 cacaaaaaat ccggtcgaac ataggaaaaa tggcaatagg cgtggcattc tcaggaaaaa
     2281 gaaagaaata tgccggctta aaagccggcg tttccaaaaa gagtaaccca cacacgtgcc
     2341 ggactaaagt tcagcgctct cagggcgcta ccggaaaggc gaaaaagcga aaaccataac
     2401 cctgccgaac taaagttcgc cattcccatt ttggcgcacc tcgatattat acccacactg
     2461 cctatccgtg cgttcccacg tttagccttt ctccgtattg gaacgtgctc gcgcttatcg
     2521 gtattccttc aaccaatcga ccaattcttc tcgcgtaaag gatttcggat tgagtttggt
     2581 gataggcgag cgcatcgttt gatccgctat gctttccaac tcttcttcct gcacacccac
     2641 ggtactcaaa cgcgtcgggg cgttgataga ctgcatgagc ccttcgatcc tctccaaaag
     2701 agcgtcgagt gacggggacc ccagatagtc caataaacga gccattttct ccgggatcag
     2761 cggggcgttg gtttggagca tcggttttaa tagtacggag caagcgaagc cgtgttccag
     2821 atggtagtcg accgtcagcg gataactcag ggcgtgaagg atcgtcgtcc tcgtctgact
     2881 aaaagcgata ccggccatca agctccccag cgacagattc gtccgcgcca ccctatcctc
     2941 gggattgcga tagccttggg ttagattgtc cagtatgagt ttgatcgcct ccaaggcgat
     3001 cggctcgcta aagggtgtcg aactgagcgc ccaataagac tcgacggcgt gtacgagcgc
     3061 gtccagtccc gttgcagccg tatgtttgtc gggtaccgtc agggtcaatt ccgggtccac
     3121 gtaggcgtag tcgttccaaa accacggcga cactatagac ttctttagcc gattcgcggg
     3181 atcggagaag acggccacgt tcgtcacttc gctgccggtt ccagaggtag tcgggatggc
     3241 gatcaactgc gtatgccgtc gagtgaacgc gcgttcaccc gagagatacg atcggatccc
     3301 cgacgaggcg tccaggagct cttcccgctc gtcacgtaaa caggcgatcc ctttcgccgc
     3361 atccaacgcg ctcccaccgc ccaacgcgat gatcacttcg atctcgttaa cgcgcgccag
     3421 gaatccgccc ttatccacgc tttcgcaggt cgggttgggc tcgatctcgt cgaaaacgat
     3481 cagatcggcc ggcgtacccg cctcccgtat gattctgttc agtatcccgg tgcgcctcag
     3541 acctgtggtc acgaccatga cccgcttttg tgcgcacagt tcaccgatac cttgcgtgac
     3601 gcccacgcca aaaaacgttt tcgtgggcaa gaagaataga aaagaccgat ttgtcacgtt
     3661 gtctgtttca cctcaccatt tcttatagat ttgtgggtct tgacggctga tatacatccg
     3721 ttcggtcatc gtgagggtgt tttcgccgag gggaaatatg tcaggcggcg gcaccgttcc
     3781 ggctcctttg accttccaga agcagtgata gaagagcgac cactcggttt cgatgccgtg
     3841 agtgttcgca tagttttgta ccgctgtcca ccctgcgccc gtggaccaga aagatagctg
     3901 ttgcaaaaag tcgctcgtcc actcgacata agaatcttcc ttgttgtggt cgtgcaagac
     3961 gctaacgggt aggccatctc caaagatgcc gatcaacgtc aattgatccc aatcggggta
     4021 cgcgaatttg tcagcggaag tggacgcgat gaagaggcct aagttctcga ggattctgga
     4081 ggtagttccg tcgaagttat cgagccagct gccggcatga caggtttcca agatcaccga
     4141 aaacagcaca tcaggataac tattgatcaa agtgtgcaac tgagacgccc tgtagctcgt
     4201 gccgcctatg gaaatcctat cgttgcctcc gtgtccgatt atatacaaaa cgatattgcg
     4261 gcagtcccga tttacgacta aatattcaac ggcctgtagt atttgttccg cggggttcgc
     4321 cataaccgtg gggctcgtca ggtctcgcac gttattgttt ccgaaaagcg tcttcatatc
     4381 gttcaaaaat tgtttggaga tcgctttcgc ttctgcggac aaggtttcct ccgtcaaaca
     4441 cccattcacg accaccgcgc ctttggcgga tgcggaaatc gccgaaggga accaggtttt
     4501 gttgtgggtg tcgggtttac accacgcgta gggattccaa acgatcgctc caaagtatct
     4561 gtcggaagtg gcgctattca gttggattgg ccggttacca ttgaccgtcg gccatccgtt
     4621 cgtcggctca ttcacgagga tggcgcccga ggtgctgacc acgacgatcc tgcccgggtg
     4681 ctcgtaaaac gctccaggac actcgtcgac ataaaacagc cagccatttt gcgtgagcgg
     4741 ctcacccgca cgctgtgggg attccatcag cggtgaatta gactcgataa catcgccctt
     4801 cttgacctca cctaagaaca atccgaccat aggggtctcc ggcgggtttg ggtagccgtt
     4861 atcgtttagc cacgacaaaa agcgatcgat cgctccgctt tcgttataga ccaaagtgct
     4921 ggtacaaccg gtgagggcaa ggatgagaac agcgatgaga agcagcgtaa agcaagctct
     4981 tttcattgga tattcctcct ttgcgttttt gtataaaaca gtgtagaccg cgcttacggc
     5041 gtggtcaacc gactgcctac ctcccgggtc ttataagaag gctccgcgaa agtctgtgcc
     5101 cataaataag gtaaagcaag acctagaaat agatttaggt gattataaca caaaccgcgc
     5161 ccgatgtgga gagagatccg cgatggaggc tattgagtgg gagcgttttt ctgggaacgc
     5221 cttgggagcg cccttgctgc gcgcgggaac gccctgggaa cgccgaactt tatttcggcg
     5281 ttcccaggcc ttaccaggtc ggcgtttcag ggcgcgccga cgggtgtggt ataatggtct
     5341 atcatcgcgg tttttccgcc gttttggagg agttttgaaa aaagcgttcg gattcgttat
     5401 cgccttcggg ctgtgtgtgt gggcgctgag cgcgggcggc ctttcttcac tttttcccgc
     5461 taaagttcaa aaaacaccca cgtacatcgg cgacttgctg attgtggata tccctccgtt
     5521 ctcacgagtg ttgtctttcg aagggttgac cgatgtgaag gagctcaaag acaaagtgat
     5581 cgcccaactt gccttgccga agtggtcggt agtccttttc cgtaacggat tgctcggtca
     5641 ccgcgatgtg ttcggcggtc aagtggcgat gacggattca gaccacgacg gctttccaga
     5701 tgaagccgaa ctgtgggggg acgatgcgac tcgctttcgt cactggttcg tctacatcgc
     5761 tatgcaacag aaggtc
//
LOCUS       DAOPXN010000142         5775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13839_length_5775_cov_5.295455, whole genome shotgun sequence.
ACCESSION   DAOPXN010000142 DAOPXN010000000
VERSION     DAOPXN010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13839_length_5775_cov_5.295455"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1099)
                     /gene="trmB"
                     /locus_tag="PKM49_07770"
     CDS             complement(116..1099)
                     /gene="trmB"
                     /locus_tag="PKM49_07770"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNY82740.1"
                     /translation="MIDKTYQTGLTEYGYLIQPSESERFPLDWATEFGNHNPIEVEIG
                     FGYGEFLKIVAQKDPGHNFVGIETSIFSAVKAQRKFVAAGLGNIRLILLDARFALRNL
                     FGPASVSKVYLNFPVPWSKNRHAERRVIVPEFFSTLNDVLAEAGVFEVSTDVGSYAQE
                     VVSLGQAAGFRLLIWETNPDRESLTRYERKWKRFDRDIHFVQLQKERTETVTRALEGM
                     VPLPHLFIKREEIDLSSLSKILDKTCKSEDGKQICVFKSIFKDLDRDHYLVKTITTDE
                     GFVQHCLLAIVGREQNWLVKLDGTTNPYRTPAVKFAVKQLAKALVRNESDD"
     gene            complement(1106..2527)
                     /gene="gltX"
                     /locus_tag="PKM49_07775"
     CDS             complement(1106..2527)
                     /gene="gltX"
                     /locus_tag="PKM49_07775"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNY82741.1"
                     /translation="MTRLRFAPSPTGYLHVGGVRTALFNWLFARKTGGQFILRVEDTD
                     QERSTKAYEQEIYDSLRWCGLLWDEGPDIGGEYGPYRQMERHEKGYYRDAAQRLLENK
                     RAYYSVARVADPKTEVLRSDRYPEGYSEEEYLIAVKFAVPQGTTCFHDLLKGDLRFEN
                     QTIDDFVILKSSGIPVYNFAAVVDDTMMRITHVIRGEVHISNTPRQIMLYQALGLELP
                     VFMHLPLILGADKTPLSKRHGGTSVEFFRKEGYLSEGLMNYLALLGWNVKQEVFQPLQ
                     ELEDFYLESISNKSVVFDYKKLEWINGKHVRSLPVGTFSTLFKEWIDRYRKAYPEIAE
                     LIVRQPQGRVERILLAGQEKVNTFVQLLPLLRPLLAEADVLQYDEKWTEKYLKKPETP
                     ALLEKFEREFSKNPIDTYEKIKSIFDRNIEESGLSTNQVYQTLRGALTGSLVSLGLIE
                     TVDFLGREEVLKRIHNALALLKV"
     gene            complement(2524..3063)
                     /gene="folK"
                     /locus_tag="PKM49_07780"
     CDS             complement(2524..3063)
                     /gene="folK"
                     /locus_tag="PKM49_07780"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNY82742.1"
                     /translation="MAEDTRTSGSEHAVYLALGSNVGDRKRHLQCALRALEKKGARIV
                     LKSDLYSSKPYGYEDQGDFYNMAVGVLTSLPPFSLLQTLKEVERECGRNPTFRWGPRE
                     IDIDIIFFGNLVLSDVELTVPHPDFRERDFVLRPLMDIGRMIVDPLTEKTVEELYGLL
                     TKDTCVNLGSLDAEEGVSE"
     gene            complement(3069..4169)
                     /locus_tag="PKM49_07785"
     CDS             complement(3069..4169)
                     /locus_tag="PKM49_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82743.1"
                     /translation="MDKESAVKVRLFVSCFLIACFSWVFAGETPVLTLRSEVALEGKC
                     VQLFVDETYGRAFVIDYLNHTVHVVDLVEERVFHRLTQSSYPVYLVFHADRIYVSDFW
                     GHKVDAYDRESLLLISSIPCKRGPGYMLERGGLLYVASQLEPVLQVFYLPTGQELYAF
                     PLEGRVPKFYLLDDLVILPYYDNYHTWSRDFEMVDSVAFLNPTTFTRWNIEGKIKKPL
                     HILSLESGKYLIAGYLDSAIWRVDWGQKEVTEYITWKGHSHIMDVALFAGKIAITSMS
                     EDDLFYLDPTSKELKRITLGGGIIDLEPYRDQYLFAAGNFDNLLYVLDGDYRLVETKP
                     LKDYPIAMKVYDNRLVVLNMDGPILATYEIGR"
     gene            complement(4148..5104)
                     /locus_tag="PKM49_07790"
     CDS             complement(4148..5104)
                     /locus_tag="PKM49_07790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNY82744.1"
                     /translation="MRKNPLTKGILIINIFMFAMSAVFSGGSFLKSLWSGGDLQTLLK
                     SGASFGAYVQQGQVYRLVSSLFLHAGLIHLIFNSYALYMFGTIVESIFGSRRFIVVYL
                     ASGFVGALLTQLFYPGVVSVGASGAIFGLIGQLFSLGLRKDTPRRLTPVTGTALLPMI
                     IINLLLGFTVPGINNMAHIGGFATGFLFGLFLAPFRIAARHWSILWTVLSVLCVVVSL
                     VCISYVFLFPEPDIEQIINFANQYAEVLTLLSGTQNLRSDSYYLELLRPFDGATRALK
                     EDVQRYIETGGHSDTLYNLQLRFKAWQAIVLKKYGTWIKKAP"
     gene            complement(5457..>5775)
                     /locus_tag="PKM49_07795"
     CDS             complement(5457..>5775)
                     /locus_tag="PKM49_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HNY82745.1"
                     /translation="TVGRNLSVAPGMTQKDDMIKGIKKGVLLGRFSGGAPAPNGDFSG
                     VAKNSYYIEDGKILYALKETMIAGNVRKMLFDIKGISAERCRFGYATLPWLHVDGINV
                     VTA"
BASE COUNT         1395 a         1612 c         1448 g         1320 t
ORIGIN      
        1 cccgcacagt ccctcaagga ccaaaaagaa gaggaagctc cccgcgtctc agaatccccc
       61 gcatgcgaca ccactgaaac gcaagaaacg gtaaattcgt cgacaggagc gagatttagt
      121 cgtcgctctc gtttcgaacc agggctttgg ccaactgttt caccgcgaac ttcacggcgg
      181 gcgtacgata gggatttgtc gtgccgtcca gcttgaccag ccaattctgt tctcgaccca
      241 ctatggcgag aaggcaatgc tgaacaaacc cttcgtcggt cgtgatcgtc ttcaccagat
      301 aatgatccct atcaagatct ttgaaaatgg atttgaagac acagatctgt ttgccatcct
      361 cggatttaca ggtcttatcg aggatcttcg aaaggctcga gagatcgatt tcttcccgtt
      421 tgatgaatag gtgaggcaaa ggcaccatcc cctccagagc gcgcgtcacc gtctccgttc
      481 gttctttttg cagttggacg aagtggatgt cccggtcaaa acgtttccac ttacgctcgt
      541 aacgggtcag ggattctcgg tccgggttcg tctcccaaat caaaaggcga aaccccgccg
      601 cttgcccaag tgaaacgacc tcttgcgcat aactccccac atccgtcgat acctcgaaaa
      661 ccccggcttc tgccaaaaca tcgttgagcg tcgagaagaa ttcgggaacg atcacacggc
      721 gttccgcgtg tcggtttttt gaccagggaa ccgggaaatt cagatagact tttgaaaccg
      781 aggcgggccc gaatagattt ctcaacgcaa aacgcgcgtc caagagaatc aaacggatat
      841 ttccgagacc cgccgccacg aacttccttt gcgccttgac ggcggaaaag atggacgttt
      901 cgataccgac gaagttgtgg ccagggtcct tctgggcaac gatcttgagg aattcgccat
      961 acccaaagcc tatctcgact tctatcggat tgtggttgcc aaactccgtc gcccaatcga
     1021 gcgggaagcg ctctgactcg gagggctgaa tcagataacc atattccgta agaccagttt
     1081 gataggtttt atcgatcaca ggatcctata ccttgagaag cgccaaggcg ttgtgtatcc
     1141 gctttaagac ttcttcccgc cccaaaaaat cgacggtttc gatgaggcct aacgagacga
     1201 gcgaacccgt gagcgcgcct ctcaacgttt gatagacctg attcgtcgac aaaccgcttt
     1261 cttctatatt ccgatcgaaa atagacttta tcttttcgta ggtgtctata gggtttttcg
     1321 aaaactcccg ctcgaatttc tcaagcagcg cgggcgtttc aggtttcttc aggtattttt
     1381 ccgtccactt ctcatcgtac tgcaagacgt cggcctcggc gaggagcggc cttaagagcg
     1441 gtaacaactg aacgaaggtg ttcaccttct cctgtccggc caataaaatc cgctcgacgc
     1501 gcccctgggg ctgtcgaaca atcaactcgg cgatttcggg ataggccttg cgataacggt
     1561 cgatccactc tttaaacaac gtcgaaaaag tcccgacggg aagcgaacgg acgtgctttc
     1621 cgttgatcca ttcgagtttc ttatagtcga aaaccacgga cttgttggaa atgctctcca
     1681 aatagaaatc ctccagctcc tgcaacggtt ggaagacttc ttgcttgaca ttccagccga
     1741 gcaaggccag gtagttcatc aacccttcgg acagataccc ttctttgcga aagaactcta
     1801 ccgaagtccc gccgtggcgt ttactcagag gggtcttatc cgcgcctaaa atcaagggaa
     1861 gatgcataaa cacgggaagt tccaaaccca acgcttgata gagcataatc tgccgagggg
     1921 tattcgagat gtggacctcc ccccggatca cgtgggtaat ccgcatcatc gtgtcatcga
     1981 ctactgcggc gaaattgtac accggaatac cgctcgattt cagtataacg aaatcgtcta
     2041 tggtctgatt ctcaaaccga aggtcgcctt tcaacagatc gtgaaaacaa gtcgtccctt
     2101 gtggaaccgc gaacttcacg gcgatcaggt attcctcttc cgaataccct tccgggtaac
     2161 gatcggaccg gagcacctcc gtttttggat cggccactct cgccaccgag tagtaggcgc
     2221 gcttgttttc caacagcctc tgagctgcgt cccgatagta ccctttctcg tgccgttcca
     2281 tctggcgata ggggccgtac tcccccccga tatccggacc ctcgtcccat agcagcccac
     2341 accaacgcag ggagtcgtaa atctcctgct cgtacgcttt cgtcgaacgc tcttgatccg
     2401 tatcctctac acgcaaaatg aactgtccgc cggtttttcg cgcgaagagc cagttgaaca
     2461 gggccgtacg aacgccgccg acgtgaaggt aaccggttgg gctcggagca aatcgtaacc
     2521 gcgtcattcc gacacccctt cctctgcgtc cagcgaaccg aggttgacgc aggtatcttt
     2581 ggttaatagg ccatagagtt cttccacagt tttctcagtc aacggatcga cgatcatacg
     2641 cccgatgtcc atcagcggcc gcagaacaaa atcccgttcg cggaaatccg gatgcggcac
     2701 cgtgagctcg acatccgaga gaaccagatt gccgaaaaag atgatatcga tgtcgatctc
     2761 gcgcggcccc cagcggaaag tcgggtttct gccgcactct cgctccactt ctttgagcgt
     2821 ttgcaacaaa gagaacgggg gtaatgaggt cagaacgccc accgccatat tgtaaaagtc
     2881 accttgatct tcataaccat agggtttcga ggagtaaagg tccgatttta gaacgatccg
     2941 cgcccctttt ttttcgaggg ccctcaaagc gcattgaaga tgacgctttc ggtcacccac
     3001 attgctgccg agcgccaaat acacggcgtg ttccgagccg gaggttcgtg tgtcttctgc
     3061 catatgtttt accttcctat ttcgtaggtc gccaagatgg gaccatccat attgagcaca
     3121 accagccgat tgtcgtagac cttcatcgcg atggggtagt ccttcaaagg ctttgtctcg
     3181 accaatcggt agtctccgtc aagcacgtac aagaggttgt cgaagttacc ggccgcgaac
     3241 aagtattgat cacggtacgg ctcgagatcg atgatacccc cgcctaacgt gatccgtttc
     3301 agctctttgg acgttgggtc caaatagaag aggtcgtctt cgctcatcga ggtaatcgcg
     3361 attttccccg caaacaacgc cacgtccata atatggctgt gtcctttcca ggttatgtat
     3421 tccgtgactt ctttctggcc ccaatccacc ctccaaatgg cgctgtccag gtaccccgct
     3481 atcagatact ttcccgattc cagactcagg atatggagag gcttttttat cttcccctcg
     3541 atgttccacc tcgtaaaggt cgtcgggttg aggaaggcca ccgagtcgac catttcgaag
     3601 tcgcgggacc aggtgtggta gttgtcatag tacgggagga taaccaaatc gtctaacaga
     3661 tagaactttg gcacacgccc ttccaatgga aaagcgtaga gttcttggcc tgtcggcaga
     3721 tagaagacct gcaagacggg ttccagttga ctggccacat agagcaggcc gccccgttcg
     3781 agcatatagc ccggccccct tttgcagggt atcgaggaga taaggagaag gctctcgcgg
     3841 tcgtaggcgt ccaccttgtg gccccaaaaa tccgagacgt agatacggtc ggcgtggaag
     3901 acaaggtaaa ccgggtagga ggattgcgtc aggcgatgga aaacccgttc ctctactaaa
     3961 tcgacgacgt gcacggtatg gttgaggtag tcgatcacaa acgcgcgtcc ataggtctcg
     4021 tcgacgaaca attgaacgca cttgccctct agggccacct ccgatcttag ggtcaggacg
     4081 ggcgtttcgc ccgcgaaaac ccagctgaag caggcgatga gaaaacacga gacgaacaaa
     4141 cgaaccttca cggcgctttc tttatccaag tcccgtactt tttgaggacg atggcctgcc
     4201 aagccttgaa tcgaagctgc aaattataaa gcgtatcact atgccctccg gtttcgatgt
     4261 atcgttggac atcctctttc aacgctcgcg tggccccgtc aaaaggccgc aacagttcaa
     4321 gataatagct atcagaccgc aagttttgtg tgccggagag aagggtcaac acttccgcgt
     4381 attgattggc gaaattgatg atctgctcga tatccggttc gggaaaaagg aagacgtaac
     4441 tgatgcacac cagcgagacg acgacgcaga gaacggagag gacggtccaa aggatcgacc
     4501 aatgccgtgc ggcgatcctg aatggggcca aaaataaccc aaacaggaag cccgtcgcga
     4561 acccgccgat gtgcgccata ttgttgatcc cgggtacggt gaaccctagc agaaggttga
     4621 tgatgatcat cggtagtagc gccgttccag tcacgggggt caggcgcctg ggggtatcct
     4681 ttctcaaccc caaggaaaac aactgaccga taaggccgaa gatcgcgccg ctcgcgccca
     4741 ccgatacaac ccccggatag aacaattgcg ttaacaaagc gccgacgaaa ccggaagcca
     4801 aataaacaac gatgaaacgc cgggaaccga atatggactc gacgatggtc ccgaacatat
     4861 agagcgcgta ggagttgaag atcagatgga tcaacccggc atgtaagaag agagaagaga
     4921 ccaatcggta gacctgccct tgttgcacgt aggcgccaaa agacgcgccg gatttgagaa
     4981 gcgtttgcag atcgcctccg ctccaaagcg acttcaggaa gctaccgccg ctaaaaacgg
     5041 cgctcatagc gaacataaag atatttataa tcaaaatgcc cttcgtaagc gggttctttc
     5101 tcatctctct tcctctacgc taacagggtg ctctttctat tctttttgcg cctacgcgcg
     5161 ggtagggtat ttaccgctac cgctctagtg ggtcaagatc tgatttgaca taactaagag
     5221 ggttatcgcg gtgtaagtgg tttacgaccc cgcgatccgc cctttgcttt tttgcgccgc
     5281 gatggccccg tataaatgcg tatcgcgccg tcgcatgaag aaagggcgct tacgccgcga
     5341 tgaccacgtg tactaaaaac cgtgcgagtg attttggtct tgttccttga ttacacccca
     5401 taacttgtcc cacacctacc gccccgcaaa gataacgcta tgaatgcgcg gaaaggtcag
     5461 gctgtcacga cgttgattcc gtcgacgtgc agccagggga gcgtggcgta cccgaagcgg
     5521 cagcgctccg ccgaaatccc tttgatgtcg aacagcattt ttcgtacgtt ccccgcgatc
     5581 atcgtttctt tgagggcata taggattttt ccatcctcta tgtagtaact gtttttcgca
     5641 accccggaaa aatcgccgtt cggggccggc gctccgccgg agaatcgccc taacagaacg
     5701 cctttcttga tccccttgat catatcgtcc ttttgggtca tcccgggcgc caccgacaag
     5761 ttcctcccga ccgtc
//
LOCUS       DAOPXN010000143         5741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_13941_length_5741_cov_4.761168, whole genome shotgun sequence.
ACCESSION   DAOPXN010000143 DAOPXN010000000
VERSION     DAOPXN010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13941_length_5741_cov_4.761168"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /gene="der"
                     /locus_tag="PKM49_07800"
     CDS             complement(<1..1207)
                     /gene="der"
                     /locus_tag="PKM49_07800"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNY82746.1"
                     /translation="MFKVFIVGKPNVGKSTLFNKLVKQRKAIVDDQPGVTRDAVYGDI
                     WIDEHRVFRLVDTCGIFQEPEGVIESLMKERTVMSLSEADLILFLVNGQSPPTSEDFE
                     IADRLRVLGKPLLFIANKTESSKRYEDVYPQLFSLGFGEPIAISAEHKIGTAWLIDEI
                     AERMDAAESSKPSAVDEGLNVSEETIRVTITGKPNVGKSMLLNAMCGKERALVTDIPG
                     TTRDPIDERFVYNDQSYLVIDSAGIRKKTKVEYKSLESFSISRAEQAMETADVVLLVM
                     DAREPVSEQDQRIAGMLEKKGKASVIVLNKIDAMPSLSQEQQRELVETTLQSLYFIYY
                     SPVVFVSAKEKRNLDRLFDAINRSYASYTTTFSTGEINRTLERIKLILPAPTGKGKTL
                     NMLYATQVAN"
     gene            complement(1211..2905)
                     /locus_tag="PKM49_07805"
     CDS             complement(1211..2905)
                     /locus_tag="PKM49_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNY82747.1"
                     /translation="MVEGNENKEFQTMEEALEDFGSEHFIGEPKRGLPLQVTVLEIHP
                     TEILVEVGAKSEGIIPLEELVYPIARYKAGDKIDCIITYQNEEEGNIVLSERKRAYRK
                     ALETVSEAFKTQNTIKGVVQSKNRGGYTVRIFGVLDAFLPAGQAMFKREADNMGKELD
                     FEILDYKPRRRGVPNIVISRRKLFETEIEEFFNQTVEGSLVTGKVESITDFGAFIEIG
                     PVVGLIPKSEISYEKDVDIHQFLEVGKRIETKVLKISPKREKITLSLKALQNDPWENI
                     EEKYPAGAMATGKVAKILPFGFVVRFEEGIEGLVHSSEVFWTKRRIDLRSIVEEGEEV
                     EVEVLSANREKRKLNLSLKRVKGNPWENIVEKYVVNQVYDAKIVKILPNGLIAELEEG
                     ISGFVHITEISWNFLENIEESFTVGQPISVKILDINVEQQRIKLSLRKVQSDPWETAV
                     RELKKGSPITGQVVKLTNTGAIVLLDAYHIEAFLPVSQISSERIEKPSDVLKVGDQLE
                     AVVVRTVFEPEKERRSMVISVKQKAIDQERAELREYMQNQSSETTMEEKVKQKDRE"
     gene            complement(2926..3816)
                     /gene="ispH"
                     /locus_tag="PKM49_07810"
     CDS             complement(2926..3816)
                     /gene="ispH"
                     /locus_tag="PKM49_07810"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNY82748.1"
                     /translation="MTVPFNGSISRTQRVSLCVYSGFCTGVERAVRLAEGYLRERPGQ
                     ELYVTGDIVHNAGVMRRLTSIGLKRIEGDAPPVGKTVLVQAHGISPSVEEVLVKNHNR
                     IINGTCPILKANFDRFRLAEEQGFRSFLFGDRAHPEVEAIQGLLRDVVVFRDLEELSG
                     LRNRVLTTDKIALIAQSTKPVDEYRRSAKMLVEQLKPQQLFQMHFSICAATILREEEI
                     KEKAPLFSTVFIVGGKHSANTKKLVLLAREQCPRVHWVEDVEEARGYLALIQKSDTIL
                     IGGGASTPLQDIKAIEALFL"
     gene            complement(3813..4478)
                     /gene="cmk"
                     /locus_tag="PKM49_07815"
     CDS             complement(3813..4478)
                     /gene="cmk"
                     /locus_tag="PKM49_07815"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNY82749.1"
                     /translation="MTKHFSIAIDGPSGSGKSSVAKAVAKACGLFYLDSGAFYRAIAY
                     YLNHLNVDFHKEEECQEALKSIRFDISPAGITMNHSLLGEEIRTSEISQIASKVSVIP
                     QVRTFVTEVVRELSKDQNVIMDGRDIGTVVLPLADLKLFITASVEERARRRQKELAQQ
                     GHHSSFEETLEEIKERDHRDISRALSPLKPAPDAILIDTTHLSFDQVVNKVVMIVREK
                     IFL"
     gene            complement(4475..4837)
                     /locus_tag="PKM49_07820"
     CDS             complement(4475..4837)
                     /locus_tag="PKM49_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY82750.1"
                     /translation="MNDLKIQSKTEEKRVIVYISGDIDAYHSPKLKEEMDTFVNGELS
                     DIALDLSDVPYIDSAGLGTLVSILRETRNAKKALKLVGLRQNIKRIFEMTRLDKIFDV
                     YDTLEESAVGNKRVGGSY"
     gene            complement(4850..>5741)
                     /locus_tag="PKM49_07825"
     CDS             complement(4850..>5741)
                     /locus_tag="PKM49_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HNY82751.1"
                     /translation="DHFKDSGFEPIRQAIQNGGVLKGFKVAGAAPRYSRKMLEEIENL
                     AKKAGAKGLLHAKAVKETDSTGTLTLKTNFDRHLSAFEGENLMKAFSLEDRDICFLIS
                     GERKTVNVALGVLRGEIAKRESLIPKDIFRFLWVVDFPMFSYSEEERRFVAEHHPFTM
                     PYLSDLESYKDTDPTKIRAHAYDVVLNGWEIGGGSLRIHSREVQKTVFDLLGIDEAGQ
                     KSKFGFLLDAFQYGVPPHAGCAMGLDRLVAILAGEEFIRDVIPFPKTTSGICQMTGAP
                     SDVENEQLEKLSLKFIKDAR"
BASE COUNT         1235 a         1569 c         1325 g         1612 t
ORIGIN      
        1 tattcgccac ttgagtcgcg tacagcatat tcaatgtctt tcctttaccg gtgggcgcgg
       61 gtaagatgag tttgatccgt tctagggtgc ggttgatctc tccggtagaa aaagtggtgg
      121 tgtaagaggc ataggatcgg tttatcgcgt caaaaaggcg atccaaattc ctcttttctt
      181 tagcggaaac gaagacgact ggactatagt agatgaaata gaggctttgt aacgtcgttt
      241 ccaccaattc ccgttgttgt tcttggctca gggaaggcat cgcgtcgatc ttgttcagaa
      301 cgatgacgct ggcctttcct ttcttttcca gcatccctgc gattctttga tcctgctcgg
      361 agacgggttc gcgcgcgtcc atcaccaaca gaacgacatc ggcggtttcc atcgcctgct
      421 cggcgcgtga aatgctgaaa ctttccaaag acttgtattc caccttggtt ttcttgcgaa
      481 tcccggcgga atcgatgacg aggtaggatt gatcgttata gacgaacctc tcgtcgatcg
      541 ggtccctcgt cgtgccgggg atatctgtga cgagcgcgcg ctctttcccg cacatcgcgt
      601 tcagtagcat cgattttcct acgttgggtt ttccggtgat agtgaccctt atcgtttcct
      661 ccgacacgtt cagaccctcg tcgacggcgg agggcttgga agactcggcc gcatccattc
      721 tttcagcgat ctcgtcgatc aaccatgccg tccctatctt atgctcggca gagatagcga
      781 tcggttctcc gaaacctaaa gagaataact gaggatagac gtcctcgtac cgtttgctac
      841 tttcggtttt attcgcgatg aataaaagcg gttttccgag cacccgaagc cggtcggcga
      901 tctcgaagtc ttccgaagtc ggagggctct gcccgttgac caagaacagt atcaggtccg
      961 cttcggataa cgacatcacg gtcctctcct tcatcagtga ctcaatgacg ccttccggct
     1021 cttgaaaaat accacaggta tctaccaaac gaaaaacccg gtgctcgtcg atccatatat
     1081 caccatagac cgcgtcacga gtcacaccgg gttgatcatc cacgatagct ttccgttgct
     1141 tgacgagctt attgaatagc gtcgattttc ctacgttcgg ttttcctacg atgaaaacct
     1201 tgaacaaagg ctattcccga tccttttgtt tgactttctc ttccatagtg gtttcgctgc
     1261 tttgattctg catgtattcc ctcagctcag ctcgttcttg gtcgatcgct ttctgcttaa
     1321 cagagatgac catacttcga cgctcttttt ctggttcgaa aacggtcctt accacgacgg
     1381 cctcgagttg atctccaacc tttagaacgt cagaaggttt ctcgatccgt tcggaagaga
     1441 tctggctgac aggtaagaat gcttcgatat ggtaagcgtc gaggagaacg atcgcgccgg
     1501 tgttggtgag tttcaccact tgccccgtaa tcggactacc ctttttcagc tcacgaacgg
     1561 cggtctccca tggatcgctt tggaccttgc ggaggctcag cttaatccgt tgctgttcga
     1621 cgttgatatc caaaatcttt accgagatgg gttgtccgac agtaaagctc tcttctatgt
     1681 tttccaagaa attccacgag atctcggtga tgtgtacgaa gcctgagatc ccttcttcga
     1741 gttcggcgat caatccgttc ggcagtattt tcacgatctt ggcgtcatag acctggttga
     1801 cgacgtattt ctcgacaatg ttttcccaag gatttccctt cactcgtttc agactgagat
     1861 tgagctttcg tttttcccgg tttgcggaca atacttccac ttccacctct tcgccttcct
     1921 cgacgatgct ccgtaagtcg atccgccttt tcgtccagaa cacctccgag gagtgaacca
     1981 ggccttctat gccttcttca aaacggacga cgaagccaaa ggggaggatc ttcgcgacct
     2041 ttccggtcgc cattgctccg gccgggtact tttcttcgat attttcccac ggatcgtttt
     2101 gaagggcttt taagctcaag gtgatttttt ctctcttcgg agatattttt aataccttcg
     2161 tttctatccg ttttccgact tccaagaatt gatggatgtc cacgtccttc tcatacgaga
     2221 tctccgattt cggaatcagt cccactacgg gtccaatttc gatgaaggcg ccgaaatccg
     2281 tgatactttc gacctttccg gtcacgaggc ttccttctac cgtctggtta aagaactcct
     2341 ctatttcggt ctcgaacagc ttccttctgg aaatgacgat attcggcact ccccggcgtc
     2401 gaggtttgta gtcgagtatt tcaaagtcca actctttgcc catattgtcg gcctcgcgtt
     2461 tgaacattgc ctggcccgcc gggagaaagg cgtcgagaac tccgaaaatc cgtacggtgt
     2521 atccgccccg atttttcgat tgcacaaccc ctttgatagt gttttgggtt ttaaaggcct
     2581 cggaaacggt ctccagcgct ttacgataag cacgtttcct ctcggaaagg acgatgtttc
     2641 cctcctcttc gttttggtac gtgatgatgc agtcgatctt gtcgcccgct ttataacggg
     2701 cgataggata gacgagctct tcgagaggga tgattccttc gcttttcgcg ccgacttcca
     2761 cgaggatttc tgtcggatgg atttccaaca ccgtcacctg gagaggtaac cctcttttcg
     2821 gttccccgat aaagtgttcg ctaccgaaat cttccagggc ttcttccatg gtttgaaatt
     2881 ctttattctc gttaccttcg accatcttag tcaacctccc aatatttaaa gaaatagcgc
     2941 ttctatagct ttgatatctt ggagcggggt ggacgcaccg cccccgatca ggatcgtgtc
     3001 gctcttttgg atcaacgcca ggtatcctct cgcctcttcc acgtcttcta cccaatgcac
     3061 ccgcgggcat tgctctctcg caaggagcac gagctttttc gtgttcgcac tgtgcttgcc
     3121 tccgacgatg aacacggtag aaaacaacgg cgccttttct ttgatctcct cttctcgtaa
     3181 gatcgtcgcg gcacagatcg aaaaatgcat ctgaaagagc tgttgcggtt tcaattgctc
     3241 gaccagcatc ttggcgcttc gtctgtactc gtccaccggc ttcgtggatt gtgcgatgag
     3301 cgcgatctta tctgtcgtca aaactcggtt tcgaaggccc gaaagctcct ccaagtcacg
     3361 aaaaacgacg acgtcgcgca ataggccttg tatggcttcc acttccggat gcgcgcggtc
     3421 tccgaaaaga aaactgcgaa atccctgttc ttccgccagg cggaaacgat cgaagttcgc
     3481 tttcagaata ggacaagtcc cgttgatgat tcggttatgg ttcttcacca atacctcttc
     3541 aacgctcgga gatatcccgt gtgcctgcac taaaaccgtc ttaccaaccg ggggagcgtc
     3601 accttctatc ctcttgagcc ctatggaggt caatcggcgc atcacccccg cgttatgcac
     3661 gatgtcgcca gtcacgtaga gttcttgccc gggtctttcg cgtaaatatc cttccgctaa
     3721 acgaacggct ctctccacac cggtgcaaaa ccctgaatag acgcagaggg acaccctctg
     3781 tgtccgactt atcgaaccat tgaaaggaac ggtcatagaa atatcttttc tcgaacgatc
     3841 atcaccactt tatttaccac ttggtcgaaa gaaaggtggg tggtatctat aaggattgcg
     3901 tccggagcgg gtttaagagg tgatagtgct ctagaaatat ctctgtggtc tctttctttg
     3961 atctcttcca atgtttcttc gaaagaactg tgatgtccct gttgtgccag ctccttttgt
     4021 cttcttcgcg cccgttcttc gaccgacgcg gtgataaata attttaggtc tgccagaggt
     4081 aaaacgaccg taccgatgtc tctcccatcc atgatgacgt tctggtcttt ggacaactcg
     4141 cgcaccactt cagtgacaaa ggtcctaacc tgcgggatca ccgacacttt cgacgcgatc
     4201 tgactgattt cgctcgttcg gatttcctca cccaaaaggc tgtgattcat cgtgatcccg
     4261 gcaggtgata tgtcgaatcg tatcgatttt agcgcttcct ggcattcctc ttctttgtga
     4321 aaatccacgt tgaggtggtt caggtagtag gctatcgccc gataaaacgc gccgctatcc
     4381 aaataaaaca gcccgcaggc tttcgcaacg gccttcgcga cgctcgattt cccagaaccg
     4441 gagggcccat ctatcgcgat agaaaagtgt ttcgtcaata agaccctccg accctcttat
     4501 ttcctactgc ggactcctca agtgtgtcgt acacgtcaaa tatcttatcc aaacgggtca
     4561 tttcgaatat ccgtttgata ttctgtcgca gcccaactag cttcaaagct ttcttggcgt
     4621 ttcgggtctc tctcaatatc gacaccaacg tgcccaaacc cgcgctgtcg atatagggga
     4681 cgtcggagag atcgagcgcg atgtcactca actcgccatt gacgaaagta tccatttctt
     4741 ctttcaattt aggtgagtga taagcgtcga tatcgccact gatatacacg atgacgcgtt
     4801 tctcctccgt tttagattgg atttttagat cgttcatcct tcatcacctt caccgagcat
     4861 cttttataaa tttgagggat agcttttcca attgttcgtt ttccacatcc gaaggagcgc
     4921 ccgtcatttg acagattccg ctggtggttt ttgggaacgg gatcacgtcg cggataaact
     4981 cttctccggc caggatcgcg accaagcgat ccaagcccat agcgcatccg gcgtgtgggg
     5041 gcaccccata ttggaaggcg tccaacagaa acccgaactt gctcttttgg ccggcttcat
     5101 cgattcctaa caagtcgaat acggttttct gcacttcccg agaatggatt cgaaggctac
     5161 cgcccccgat ttcccaaccg ttcagaacga cgtcgtacgc gtgtgcgcgt atcttcgtcg
     5221 gatccgtgtc cttatacgac tccaaatccg aaagataagg catcgtaaag ggatgatgct
     5281 ccgccacaaa ccgccgctct tcctcgctat aggagaacat cgggaagtcg acaacccaca
     5341 agaagcggaa tatgtctttg ggaatcaagg attcgcgttt ggcgatctcc cctctaagga
     5401 ctcccaaagc gacattaacg gtctttctct ccccggagat caaaaaacag atatcccgat
     5461 cttcgagaga gaaggctttc ataaggtttt ccccttcgaa agcggaaagg tgtcggtcaa
     5521 agttcgtttt tagcgtcaga gtgcccgttg aatctgtttc cttcaccgcc ttcgcgtgaa
     5581 ggagcccttt cgcgcctgct ttttttgcaa ggttttctat ctcctcgagc atcttccggc
     5641 tgtaacgcgg cgcagctccg gccaccttga accctttaag aactccgccg ttctggatcg
     5701 cttgccgtat cggctcgaat ccagaatcct taaaatggtc c
//
LOCUS       DAOPXN010000144         5674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14188_length_5674_cov_4.814380, whole genome shotgun sequence.
ACCESSION   DAOPXN010000144 DAOPXN010000000
VERSION     DAOPXN010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14188_length_5674_cov_4.814380"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKM49_07830"
     CDS             <1..551
                     /locus_tag="PKM49_07830"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNY82752.1"
                     /translation="HLRSSFGNTIGTYIQEGETIMEQAVVRGIAHSKSEAKITVTGLP
                     RSPSAFADLFTEIAAQNINVDIVVQDFGEQATMSISYLVAREDYDAAYEITTQFTQKY
                     EGARITGEKDMGKVSVIGIGMRSHPGVAARVFRTLSNEGVSIEMVSCSEIKITCVIHE
                     KEIEKAARSLHTEFCARGEIAL"
     gene            548..1570
                     /locus_tag="PKM49_07835"
     CDS             548..1570
                     /locus_tag="PKM49_07835"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNY82753.1"
                     /translation="MNRLRVGIVGSTGAVGLEMLRLLEETNFPVSELSLFASERSVGL
                     TQLFRGQPVSIRPLSDTCFRGLDLLFNATSGELARKYLPIALEEGTRVIDNSSAFRMN
                     PEVPLVVPEVNAHRIDSSSTLIANPNCSTIQMCVAVAPFERAFGVKRIIVSTYQAASG
                     AGKEAMEELVEQTKAFVNGGALEARKFPYPLLFNLIPHIDRFEENGYTREEMKMVNET
                     RKIFEKPEWRISATAVRVPVIRAHSESITLELERDFDPETIRAVLNDTPGIVVQDDPE
                     TNLYPMPAQAALRKEVFIGRIRKDLAFEKGVSFWVVADQLLKGAAWNAIQIAQTLVER
                     VWNIRF"
     gene            1680..2843
                     /locus_tag="PKM49_07840"
     CDS             1680..2843
                     /locus_tag="PKM49_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNY82754.1"
                     /translation="MSRIRTIEEINEKIANGNAVVLTAEEIIDEVAQEGAEATLEKVD
                     VVTTATFGPMCSSGAFLNFGHSDPPIRMAKTALNDVEAYSGIAAVDAYIGATQLSETE
                     GDRYGGAHVICDLISRKPVRLSASSPGTDCYPRKRLETEITLDTINEAYLFNPRNAYQ
                     NYGAAINLSAETKYTYMGTLRPHGANVTYATCGQLSPLLNDPDYRTIGIGTRLFLAGA
                     QGYVAWQGTQFYSARPKNAAQVPVGGAGTLALIGDMKSMDPRWLRPIRLKNYGVTLCV
                     GIGVPIPILDIDMLRAVSIRNRDIQTLLFDFGIGARDRPVIATVNYEELWSGSVLIEG
                     RKTPAAPMSDIRKAREIADFLREEILARRFFLTEPVARFPRDQRLRRIENARI"
     gene            3021..3761
                     /locus_tag="PKM49_07845"
     CDS             3021..3761
                     /locus_tag="PKM49_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNY82755.1"
                     /translation="MPYEARTYRGRMVPEGLQGFNVNMEETDLMVFADGDYRPYVAER
                     VRFYRDQLKGIQNPAFFTSLIPIEDDLHYPPIARHMIQASAACGVGPMAAVAGAIAEY
                     VGLDLLNLSSEVIVENGGDIFLRTRRARKIGIYAGLSPLSNRLSLEIDAEETPMGICT
                     SSGTVGHSLSFGKADAVVTLAKDTPLADAAATAIANLVRTKEDIERAITAAQRIPGVT
                     GVVIIIGEHLGVWGQVRLCRRTRPAPTE"
     gene            4162..>5674
                     /locus_tag="PKM49_07850"
     CDS             4162..>5674
                     /locus_tag="PKM49_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82756.1"
                     /translation="MKLKSVRAKLLAYFIPVSAAVLVTVGLIVGLLARDVTLNQAVEL
                     TGGTMTAASRIMEEWLMGIKNVMGNLAITDHVRSFDPTLYTQRFTETIQNSGGVFQSI
                     ILCDMTGKAVSEKGIVADISDRNYFKEIIFQGKEFTVSEGILSRFTNDYAIHVAHVVL
                     DRNGKRIGLLGAAISLKTLSEKAASIALGKGGYTFLVDGTGMVIAHQKKDLIMKLNVL
                     EASKMGYKGLEEAGREMVRGNDGLHNIVQPTGEKQIMQYDPVPNTPNWSLVGALPEAE
                     VEEKSTFLLGAVILSFAIIVAIIVFISMFVGTLISKSMKKLALQVDQFGKGDLTTTFE
                     VKGHDEIAQIAKALSGMGYDLREAVVSIASSSKEIKSASTDLAAISEEQLALSEELSS
                     QSQSANTHLQNTASSIEEVDSGVEEVAASAQNVSKTAQALSVENEKTADGSRKGEEMA
                     SVAVQETEATAKQTLLAAEKVQKLAENSKNVGEIVDTISSIAEQTNLLALNAAI"
BASE COUNT         1438 a         1490 c         1562 g         1184 t
ORIGIN      
        1 tccacttgcg tagttcattc ggtaacacta tcggtaccta tattcaagaa ggagagacca
       61 ttatggaaca agcagtcgtg cggggcatcg cccattctaa gtccgaagcg aagatcaccg
      121 tcaccggatt acccagatcg ccttccgcgt ttgcggacct tttcactgag atagcggcac
      181 aaaacatcaa cgtcgatatc gtcgtccaag attttggcga acaagcgacg atgtccattt
      241 cctacctcgt ggctagagaa gactacgatg ccgcttacga aattaccacg caatttactc
      301 aaaaatacga aggcgctcga atcactggcg aaaaggatat ggggaaggtc tcggtcatcg
      361 ggatcggtat gcgttctcac ccgggcgtgg cggcccgcgt cttccgaacc ctctctaatg
      421 agggcgtgtc tatcgagatg gtctcttgtt ccgaaataaa gatcacctgc gtgattcacg
      481 aaaaagagat cgaaaaagcg gcccgaagct tacatacgga gttttgcgcg agaggggaga
      541 tagccttatg aaccgcttgc gtgtgggcat cgtcgggtcg accggcgcgg tgggtctcga
      601 aatgttacgt cttctggaag aaaccaactt tccggtatcc gagctctcgc tctttgcttc
      661 agagcggtct gtagggttga cccaactttt cagagggcaa cccgtgagta ttcgacccct
      721 ttccgacacc tgttttcggg ggttggacct cctgttcaat gccacttctg gggaactggc
      781 gcgaaagtat ttaccgatcg cgttagaaga agggactcgg gtgatcgaca acagcagcgc
      841 gttccgaatg aaccccgaag taccgctcgt cgttccggag gtgaacgctc atcggatcga
      901 ctcgtcctct acgctcatag caaatccaaa ctgctcaacg atccaaatgt gcgtcgcggt
      961 ggccccattt gagcgcgcct ttggggtaaa acggatcatc gtctccacct atcaggctgc
     1021 ctcgggcgcg gggaaagagg cgatggagga gttggtcgag caaacgaaag cttttgtgaa
     1081 cgggggggct ttggaggctc ggaaatttcc ctatccgctc ctcttcaatt tgattccaca
     1141 catcgatcgc tttgaagaga acggttacac ccgtgaagag atgaaaatgg taaacgaaac
     1201 acgcaaaatc ttcgaaaaac ccgaatggcg gatcagcgcg acagctgttc gcgttccggt
     1261 gatccgggcc cactcggaat ccatcacgct ggaactggag agggatttcg atcccgagac
     1321 gattcgcgcc gttctaaacg acacgccggg tatcgtggtg caagacgacc cggaaaccaa
     1381 cctctaccca atgccggccc aagcggcctt acgaaaagag gttttcatcg gtaggatacg
     1441 gaaagacctc gccttcgaaa aaggtgtgtc tttttgggtc gtggccgatc agctgttaaa
     1501 aggagctgct tggaacgcga tccagatcgc ccaaaccctg gttgaacggg tgtggaatat
     1561 acgattttag gggtcatatt cgaacctctg tgagtcgtcg ctattgtttc ttgacgctga
     1621 aacccttttt cggtaaggcc ccgacgtgac aaggttgctt tgaaagagag gagaaaacga
     1681 tgtcaagaat ccgtaccatc gaagagatca acgaaaagat cgcgaacggg aacgccgtgg
     1741 tgttaactgc cgaagagatc atagacgagg tggcccaaga aggagcggag gcgaccctcg
     1801 aaaaggtgga tgtcgtgacg accgccacct tcggcccgat gtgctcgagc ggcgcatttc
     1861 taaacttcgg gcattccgac ccacccatac gcatggccaa aacagcgctc aatgacgtgg
     1921 aggcctactc cggaatcgcg gctgtcgacg cctatatagg agcaacccag ctttcggaaa
     1981 ccgaaggcga ccgttatgga ggggcgcacg tcatctgcga cctcatctcg cgaaagcccg
     2041 tgcggttaag cgcttccagt ccgggtaccg attgttatcc gaggaaacga ttggagacgg
     2101 agatcactct ggacacgatc aacgaagcct atctgttcaa cccacgaaac gcctaccaaa
     2161 actacggggc ggcgatcaac ctttcagccg aaacgaaata cacctacatg gggactctgc
     2221 ggccccacgg ggcgaatgtc acgtacgcga cgtgcggaca gctcagcccg ttactcaacg
     2281 accccgacta ccgaacgatc gggataggca ctcgcctgtt cttagcgggt gcacaagggt
     2341 atgtggcttg gcaagggaca cagttctact ctgcgcgccc gaaaaacgcc gctcaggtac
     2401 cggtgggtgg cgccggaacc ctggcgttga tcggagacat gaaatcgatg gatccgagat
     2461 ggttgcgacc catacggtta aagaactacg gtgtcaccct gtgcgttgga atcggagtcc
     2521 cgattcctat cttggacatc gatatgctac gcgccgtctc gattagaaac agagacatcc
     2581 aaacgctcct tttcgacttt ggtataggcg cgcgcgatcg cccggtcatc gccacggtca
     2641 actacgaaga actttggagc ggatcggtcc taatcgaggg tagaaaaacg cccgccgctc
     2701 cgatgtcgga catacgaaag gcccgtgaga tcgccgattt cttacgcgag gagatcttgg
     2761 ctaggcggtt tttcctcacc gagcccgtgg cgcgttttcc gagggatcaa aggcttcggc
     2821 gcatagaaaa cgcgcgaatc tgaacccgag aagcgaatag aaagaagaga agtccctctt
     2881 aacgttgcga ccgcttgacc gtagcggttt tgagctaata gccgccgctc accgacgata
     2941 aagtcacaac cgacgtggta gaatactcgt agtacttcgt gtgcgagaat tcttctacgg
     3001 agaacgagga ggtaggctta atcccttacg aagcgcgaac gtatcgtggc agaatggttc
     3061 ccgaagggct ccagggcttc aatgtgaaca tggaagagac ggacctgatg gttttcgcgg
     3121 acggcgatta ccgtccatac gtcgccgaac gcgtccgttt ctatcgcgac cagctgaaag
     3181 gcatacaaaa cccggcgttt ttcacttcac tcattcccat tgaggatgat ctccactatc
     3241 ccccgatcgc acgccatatg atacaagcca gcgctgcgtg tggcgtcggc ccgatggcgg
     3301 ctgtcgctgg cgcgatcgcc gagtacgtcg gcctagatct actgaatctc tctagcgaag
     3361 ttatcgtaga aaacggaggg gacatctttt tacgcacccg gagggcgcgg aaaatcggaa
     3421 tatacgcggg tctgtccccc ctctctaacc gcctttcttt ggaaatagac gcagaagaaa
     3481 cgcccatggg catctgcacc tcttcgggca cggttgggca ttcgctcagc tttggcaaag
     3541 cggacgcggt ggtgacgctg gcgaaagaca ccccgctcgc cgacgccgcc gcgacggcca
     3601 tcgcgaacct cgtccgcact aaagaagata tcgaacgagc gatcacagcc gctcaacgca
     3661 tccccggagt caccggcgtg gtcatcatca tcggcgaaca cctcggcgta tggggacaag
     3721 tccgcctgtg tcgaagaaca cgccccgccc cgacggagta acctgggaac gccgcgtttt
     3781 agtgtgacat actttattct tttttgcctt ttttaaaaca gaaaccctac cagactaaag
     3841 cttggcgttc tcagggagtt ctcagcaaat atgccgaact caagttcggt gttcccagag
     3901 aggcaggccg actggaagtc ggccccacga agagaaggtg cgcttacgct gcgatgacga
     3961 cttggactta aaatcgcgcg atcgattctg accgagtttc ttgagtacac cctagaactt
     4021 gacccacacc aaccccctct tcttctcaac aatatgctat aatatcccgg tttcacagca
     4081 ctgcctaact catagggaaa cctactaata ttacaattac ggaaacacaa gtttacaaat
     4141 aaaccaaaaa ggggagaaaa aatgaagtta aagagtgtgc gcgcaaaatt gctggcgtat
     4201 tttattcccg tatcggccgc ggtgctcgtg accgtagggt tgatcgtcgg cttactggcg
     4261 agggacgtaa cgctaaacca agctgtagaa ctaacgggag gtacgatgac agcggcttcc
     4321 cggattatgg aagagtggtt aatggggata aaaaatgtga tgggtaatct ggctatcact
     4381 gaccacgtaa ggtcattcga tcccacgctg tatacacaaa gattcacaga aacgatacaa
     4441 aatagcggcg gggttttcca atcgattatt ctttgcgata tgactggtaa ggccgtttct
     4501 gaaaagggta tcgtggctga tattagcgat cgcaactatt tcaaggagat catattccaa
     4561 gggaaggaat tcaccgtaag cgaagggatt ctcagccgtt ttacaaacga ttacgccatc
     4621 cacgtcgccc acgtggtcct tgaccgtaac gggaaacgca tcggcctcct gggagccgcc
     4681 atatcgttga aaacattgag cgaaaaagcc gcatccatcg ctcttggtaa gggcggatac
     4741 acctttttag tggacggaac cgggatggtg atcgcgcacc agaagaaaga cctcattatg
     4801 aagctcaacg tgttagaagc ttcgaagatg gggtataaag gtctggaaga ggccggaaga
     4861 gagatggtac gtgggaatga tggcctacat aatatcgtcc aacccacagg agagaagcag
     4921 attatgcaat atgatcctgt accgaatacg ccgaactggt cgttggtcgg agccctaccc
     4981 gaagctgaag tggaagagaa gagcaccttc ctccttggtg ccgttatcct gagtttcgcg
     5041 atcattgtcg cgatcatcgt gtttatctct atgttcgtcg gcacgctcat ttccaaatcc
     5101 atgaaaaaac ttgccctcca agtggatcaa ttcgggaaag gggacttgac cacgaccttc
     5161 gaggtgaagg gccacgacga aatcgcccag atcgccaaag ccttgtccgg gatggggtac
     5221 gacctcagag aggcggtcgt atcgatagcg agctcctcga aagagatcaa atccgcttcg
     5281 accgatttgg ccgcgatctc ggaggaacaa ctggccctat ccgaggagct ctcttcgcaa
     5341 tcgcagagcg cgaacaccca tttgcagaac acagcctctt cgatcgaaga agtggactcc
     5401 ggtgtagagg aagtagcggc gagcgcgcag aacgtgtcaa agaccgcgca ggcgctctcc
     5461 gttgagaacg agaagacggc agacgggtcg cgtaaaggag aggagatggc ttcggtggcc
     5521 gttcaagaga ccgaagcgac ggcaaaacaa acgctgctgg cggcagaaaa ggtgcagaag
     5581 ctggcagaga attcgaaaaa cgtgggcgag atcgtcgaca cgatctcctc gattgcggag
     5641 cagacgaacc tgctggcgct caacgcggcg atag
//
LOCUS       DAOPXN010000145         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14254_length_5657_cov_5.778115, whole genome shotgun sequence.
ACCESSION   DAOPXN010000145 DAOPXN010000000
VERSION     DAOPXN010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14254_length_5657_cov_5.778115"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1084)
                     /locus_tag="PKM49_07855"
     CDS             complement(56..1084)
                     /locus_tag="PKM49_07855"
                     /inference="COORDINATES: protein motif:HMM:NF027565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4899 domain-containing protein"
                     /protein_id="HNY82757.1"
                     /translation="MNYDFYAIKIQAENRTSDDYFLGYFFGKVNSGLEYRFICFPSKY
                     VDREKTPLEMPFAEYKDYLNELYLRISSRSLEIVEMTKEFNVYLGLQIRKMGGMGAQG
                     FRFSKMAEEKNTELIRSELNDLFQNWTSDIILRFTLDFSSFSQIYEQASLSADTKVML
                     SSFGADRKDILSISDFYPLLDPMAGVTIDQYEIGSVIWCTISRFSNQTEENRLKKEYP
                     DFFDEAGGNIVPFEGTLVLKELTADGRGVLVKVMIADWFFAKSIILHNMRLLQNTQKL
                     QNRFPKLQEQSFQIGLDNPQARPASKGTTNTAHLENKKPIFDLGTVFIIFAIIALAFL
                     IYFFFFYS"
     gene            1229..2041
                     /locus_tag="PKM49_07860"
     CDS             1229..2041
                     /locus_tag="PKM49_07860"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HNY82758.1"
                     /translation="MIDSGAETVREALGKPANAKVGLVLGSGLGFMADRLEKSVKIPY
                     QKIPGFLASTVIGHEGNLVFGELSGVEVVAMQGRFHCYEGYDPADIVTPLYLLKRIGV
                     EGLIITNASGGINTDYQAGDLVVIRDIINFSFHNPLIGENDDAIGPRFPDMSTVLDVD
                     WYDRVKNRLKEKNKELKTGTYVFALGPMYETPAEIRAFRYLGADLVGMSTVPELIACR
                     HCGIRPFGISCVTNMAAGVLMQKLSHQEVMDTANRVKDTFASLVKVVVESYA"
     gene            2038..3258
                     /locus_tag="PKM49_07865"
     CDS             2038..3258
                     /locus_tag="PKM49_07865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNY82759.1"
                     /translation="MIIVTVRRQKAPKIRNRYGWVFADELVDIQADRDLGIANVFEEN
                     GGFLGKGLFLKGASKTVRMLTLRDEEIDEPFFLRRFRRALSVRTRINPNVAYRLINAE
                     SEGLPGLIVDRYGDYLVAQIRHPALEEKKPQIISALRALYEGGIRGIFERSDFESTPE
                     YRLNRHSGVLWGEVPEETTVWVDGIAYLIRFKTGQKTGLFLDQQASRAKARQLIRTYH
                     LEGQPALDLFCYTGGFSFTMAQEGMTTLGVDKSAPDIETAQRMAEINGLTGQTGFIVD
                     DVFSWLLNQTMEKPPLGKPYGLIVIDPPSLIKSQQERPYGKRLLTELVRSVLPLLAPN
                     GLICLCSCSYHLGWEEMIESVRRASADCGKAVIVVDQNVQSPDHPWLLQMPETLYLKC
                     LWMTVPVEGETERR"
     gene            3302..5368
                     /locus_tag="PKM49_07870"
     CDS             3302..5368
                     /locus_tag="PKM49_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNY82760.1"
                     /translation="MLKEIYDYLKNKAKTSSGYYAIPYLWASQSVINAMMPSDRPVGD
                     DRKPLLVNPHKYYSRILSEILEQKKPGVHYERPFSALKEQAGRSAFTGERDWLQSRYM
                     YGFFVRATTAYNHKGYWKFQGSQIGRYTEGGTFLKMIALLPYLKKIGIDTLYSLPITK
                     YSHLYQKGELPSPYAVKNFLQIEPSYKDALLAGFTVEDEFSAFVEAAHMMGMRVILDF
                     VPRTCARDNDLILEHPDWFYWIRASKEEQYKAPSIDALGFCQPSVENLSTIYAAEKTK
                     ELLGLFSHSPDRLDPQRWEYFKEEHRGKEESFFEDLVKTFGVLPPPGFSDWVNDTQPA
                     WTDVTFLKLFHLPPNPSRALVGAEQPPYVLFDIIKASNFENDSPNLPLWEYLVNVIPS
                     YQKRFGIDGVRLDMGHALPRQLEEEIIRKARDLDPGFVFIAEELEITKDERALERGYD
                     AILGNSWWMEPRVDEGKCFEFCQQLLPALKLPALVTAETPDTPRAIARKYRKRFSQFS
                     FILNLFLPNGITFINSGFEIGEVQPLNLGLDNDERGRFALPEQDPLYGKIAFFDHYAY
                     HWDQADLELLQLMQSAATLKAECGPWCRGENRLEGLFTPSEKVIAHLYKNPVQNQALI
                     LLANTDFVNASWISIDVSHIPDMRVGGVRREYVNYAKTQQYMNLEDGYVHVFLEPGEA
                     TVLTVR"
     gene            5431..>5657
                     /locus_tag="PKM49_07875"
     CDS             5431..>5657
                     /locus_tag="PKM49_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HNY82761.1"
                     /translation="MKEYLEKIETVLQRYTEVMRILSDPDVIHNRERFLEYSRERAQL
                     EELQSLYDNYRRAMKEYEENQELLQSEDSEIA"
BASE COUNT         1371 a         1397 c         1560 g         1329 t
ORIGIN      
        1 aaccgcgcgc gactctcttc tttccaggtc gggtacgtgg gcccctgcgg atacttcagg
       61 aataaaagaa aaagaaatag atgagaaagg ccaacgctat gatagcgaag atgatgaaga
      121 ctgtcccgag atcgaagatc ggttttttgt tttcgaggtg ggcggtgttc gtcgtaccct
      181 tcgaagcggg tcgggcttgc gggttatcca aaccgatctg aaaactttgc tcctgcaatt
      241 tgggaaaccg gttttggagc ttctgggtgt tttgcagcag gcgcatattg tgcaggataa
      301 tcgatttggc gaaaaaccag tcggcgatca tcactttaac gaggacgcca cgcccgtccg
      361 cggtcagttc tttgagtacc aacgtgcctt cgaaagggac gatgttgccc cccgcctcgt
      421 cgaaaaagtc ggggtactct ttcttcaacc ggttctcttc agtttggttc gagaagcgag
      481 agattgtgca ccagatcacc gagccgatct cgtattggtc gatcgtgaca ccggccatcg
      541 ggtcgagaag cgggtagaag tccgagatcg agaggatatc tttgcggtct gcgccgaaag
      601 agctgagcat aaccttcgtg tccgccgaaa ggctcgcttg ctcatagatt tgagagaagc
      661 tggaaaagtc gagtgtgaag cgcaggatga tgtcgctggt ccagttttga aagagatcgt
      721 tcaactccga ccgaatcagt tcggtgttct tttcttccgc catctttgaa aaccgaaatc
      781 cttgcgcacc cataccgccc atttttcgta tttgaaggcc gaggtaaacg ttgaactcct
      841 tggtcatctc gacgatctcc agagatcgtg aggagatacg aaggtagagt tcgttcaagt
      901 agtctttata ctccgcgaag ggcatttcca gaggggtttt ctcgcgatct acgtatttac
      961 tcggaaaaca gatgaaccgg tattccaaac cgctattgac ctttccgaag aaatacccca
     1021 agaaataatc gtccgaagta cggttttccg cttgtatttt tatcgcgtaa aaatcatagt
     1081 tcataacggg ttctcccccc tcacgacata agaccggaaa aaagcgttgt ttcggagtat
     1141 tataaactaa aaatgctata atagactcca aaaagggcag atttgtatct gcagaagcga
     1201 ggtgtctggt ttgaagcgtt tatcaagcat catcgacagc ggcgctgaaa cggtgcgaga
     1261 ggccttggga aagccagcga acgccaaggt gggattggtc cttggctctg gacttgggtt
     1321 tatggcggat cggttggaaa agagcgtaaa aatcccttac caaaaaatcc ccggctttct
     1381 cgcttcgacg gtgatcgggc acgaggggaa cctggttttt ggcgagcttt caggagtcga
     1441 ggtcgttgcg atgcaagggc gattccactg ttatgagggg tacgaccccg ccgacatcgt
     1501 cacccccctc tatctgttaa aaaggatcgg cgtggagggt ttgatcatta cgaacgcctc
     1561 cggaggcatc aacacggact atcaggccgg ggacctcgtc gtgatccgag atatcatcaa
     1621 cttctccttt cataaccctt tgattggaga gaacgacgat gcaatcgggc cccgatttcc
     1681 cgatatgtcg acggtcttgg atgtagactg gtacgatcgg gtgaaaaatc gcctcaaaga
     1741 gaagaacaaa gagctgaaaa ccggtaccta tgtgtttgcg ttgggaccga tgtacgaaac
     1801 cccggcggag attcgcgctt tccgttatct gggggcggac ttggtgggga tgtccacggt
     1861 tccggagttg atcgcctgcc gacactgtgg tattcgcccc ttcggcatct cttgcgtgac
     1921 gaatatggcg gccggcgtgc ttatgcaaaa actctcgcat caggaagtca tggatacggc
     1981 caatcgcgtg aaagacacct ttgcgagcct ggttaaagtg gttgtcgaat cttacgcatg
     2041 atcatcgtaa ccgtccggag gcagaaggct ccgaagattc gaaaccgtta cggatgggtt
     2101 tttgccgatg aactcgtcga catccaagcg gatcgggacc tagggattgc gaacgttttc
     2161 gaggaaaacg gcggctttct cggcaagggg ttattcctaa aaggcgcctc aaaaacagtc
     2221 agaatgctga ccctccggga tgaggagatc gacgagccct tttttttgcg ccgtttccgt
     2281 cgagcgttgt ccgttcgtac ccggattaac cccaacgtgg cttatcggtt gatcaacgcg
     2341 gaatccgagg ggttgcccgg gttgatcgtc gaccgttacg gggattattt ggttgcccag
     2401 attcgccacc ccgctctaga agaaaagaag ccgcagatca tatcggcctt acgtgcgctt
     2461 tacgaagggg gcattcgtgg catcttcgaa cgcagcgatt tcgaatccac gccggagtac
     2521 cgcttgaata gacattcggg cgtgctttgg ggagaggtcc cggaagagac cacggtttgg
     2581 gtggacggta tcgcctacct catccgattc aaaacgggtc agaagacggg actgtttctt
     2641 gaccaacaag cgtccagggc gaaagcccga caattgatac ggacctatca tttagagggg
     2701 caaccggcat tggatctgtt ttgttatacc ggcggctttt ctttcacgat ggcgcaagaa
     2761 ggaatgacca cccttggagt cgacaagtca gctcctgata tcgaaaccgc gcagcgtatg
     2821 gctgagatca acgggttgac cggccagacc ggcttcattg tggacgacgt tttttcctgg
     2881 ctgttgaatc aaacgatgga aaagccgccg ctaggaaagc cctacgggtt gatcgtcatc
     2941 gacccgccat ctttaattaa atcgcaacag gaacggcctt atggaaaacg cttgctcacc
     3001 gaattggtga ggtctgtgtt accgctcttg gctccaaacg gccttatctg tttgtgcagt
     3061 tgttcttacc atctcgggtg ggaagagatg atcgaatcag tccgaagggc gtctgccgat
     3121 tgtggcaaag cggtgatcgt cgtggaccaa aacgttcaat ctccggacca cccttggttg
     3181 ttgcagatgc cggaaacgct ttatttgaaa tgtttgtgga tgaccgtgcc cgtcgaaggg
     3241 gaaacggagc gacgatgatg ttggtgatgt ggatgatgtg gataaagagg tgtatcggtc
     3301 tatgttgaag gaaatttacg attacttgaa aaacaaagcg aaaacgtcca gcgggtacta
     3361 tgcgattccc tatttgtggg cttctcagag cgtgatcaac gctatgatgc cctcggaccg
     3421 tccggtcggc gacgatcgga aaccgctctt ggtgaacccg cacaaatact acagccggat
     3481 tcttagtgag attctggaac aaaaaaagcc cggagtccac tatgaacgtc ctttttccgc
     3541 tctgaaagaa caggcgggcc gctcggcttt cacgggagaa cgggattggt tgcagagccg
     3601 ttatatgtac ggcttcttcg tgagggcgac gacggcctac aaccataaag ggtactggaa
     3661 gttccaaggt tctcagatcg gtcgttacac cgaaggcggg acctttctca aaatgattgc
     3721 gcttttgccc tatttgaaaa agatcggaat cgacacgctc tattcccttc cgatcaccaa
     3781 atacagccac ctttaccaga aaggggagct tccctctccg tacgcggtca agaatttttt
     3841 acagatcgaa ccatcgtaca aagacgcctt gctcgcgggc ttcaccgtag aggacgagtt
     3901 ttcggctttc gtcgaggcgg cgcatatgat ggggatgcgg gtcattttag acttcgtccc
     3961 cagaacctgc gcgcgagata acgatctcat cctcgagcat ccggattggt tctattggat
     4021 tcgcgcctcg aaagaggagc agtacaaggc gccgtctatc gacgctctcg gcttctgcca
     4081 gcccagcgtc gagaacctct ccacgatcta cgcggcggaa aaaacgaaag aactactcgg
     4141 tctgttctcc catagcccgg atcgcttgga cccgcaacgc tgggaatact ttaaggaaga
     4201 acaccgaggc aaagaagaaa gtttcttcga agacctcgtg aaaaccttcg gggtgcttcc
     4261 ccctccgggt ttttcagatt gggtcaacga cacgcagccg gcgtggacgg acgttacgtt
     4321 cttgaagctg ttccatcttc ccccaaaccc aagtcgggcg ctcgtgggcg ccgagcaacc
     4381 cccctacgtg cttttcgata tcatcaaagc gagcaatttc gaaaacgata gccccaatct
     4441 acccttgtgg gagtatttgg taaacgtgat tcccagttat cagaaacggt tcggaatcga
     4501 cggtgtgcgt ttggatatgg gacacgccct accccgccag ctggaggagg agatcatccg
     4561 taaggcgcgc gacttggatc cgggcttcgt tttcatcgcc gaagagctgg agatcaccaa
     4621 agacgagagg gcattggaac gcggctacga tgctattctt ggaaactcct ggtggatgga
     4681 acctcgggtg gacgaaggaa agtgttttga gttctgtcag caactgctgc cggccttaaa
     4741 gctccccgcc ctggtgacgg ccgaaacgcc ggacaccccg cgggccatcg cacggaaata
     4801 ccggaagagg ttttctcagt tctcctttat cctgaacctg tttctgccca acggcatcac
     4861 cttcatcaat tccggattcg agattggaga ggtgcaaccg ctgaacttgg ggctagacaa
     4921 cgatgagaga ggacggttcg cgctgcccga acaagaccct ttgtatggta agatcgcgtt
     4981 tttcgaccat tacgcctatc actgggacca ggcggatttg gagctgttgc aactgatgca
     5041 atccgcggcg acgttgaaag ccgaatgcgg cccatggtgc cgaggggaga atcgattgga
     5101 agggctcttc accccctccg aaaaagtgat cgcgcatctc tacaagaatc cggtccaaaa
     5161 ccaggcgttg atcctcctgg cgaacaccga tttcgtgaac gcctcttgga tatcgatcga
     5221 cgtttcgcat ataccggata tgagggttgg cggtgtcaga cgggagtacg tgaattatgc
     5281 gaaaacacaa cagtatatga atctcgaaga cggatacgtc cacgtcttcc tggaaccggg
     5341 agaggcgacc gtcttaaccg tgcgttgaaa cacacgcgca tcgaggtatt ttccggatga
     5401 ccctcatccg gtagaacata gaggatggtt atgaaagagt atttggaaaa gatcgaaacg
     5461 gttttacaac gatatacgga ggttatgagg atactatccg atcctgatgt gatccacaac
     5521 cgagaacggt ttctggagta ttctcgagaa cgagctcaac tggaggagtt acaatcccta
     5581 tatgacaact accgccgggc tatgaaggag tatgaagaaa accaggagct cctacagtcc
     5641 gaggactcgg agattgc
//
LOCUS       DAOPXN010000146         5635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14317_length_5635_cov_5.682796, whole genome shotgun sequence.
ACCESSION   DAOPXN010000146 DAOPXN010000000
VERSION     DAOPXN010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14317_length_5635_cov_5.682796"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="PKM49_07880"
     CDS             <1..200
                     /locus_tag="PKM49_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82762.1"
                     /translation="ERIESGEVKLTVYLFRAVVAPDKGTLLRRYKEAMQALKEVSGRR
                     DIEDLIEQGFGISLARNQRSS"
     gene            254..475
                     /locus_tag="PKM49_07885"
     CDS             254..475
                     /locus_tag="PKM49_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82763.1"
                     /translation="MTLAKRLIDRGMKKGLEMGKADVIWKQMIKKFPNLQAAYLDKLK
                     QLDEIRLDILALELLDIQSEEELKNHLPM"
     gene            922..997
                     /locus_tag="PKM49_07890"
     tRNA            922..997
                     /locus_tag="PKM49_07890"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Pro,seq:tgg)
     gene            1004..1079
                     /locus_tag="PKM49_07895"
     tRNA            1004..1079
                     /locus_tag="PKM49_07895"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1037..1039,aa:Gly,seq:tcc)
     gene            1091..1167
                     /locus_tag="PKM49_07900"
     tRNA            1091..1167
                     /locus_tag="PKM49_07900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1125..1127,aa:Arg,seq:tct)
     gene            1192..1267
                     /locus_tag="PKM49_07905"
     tRNA            1192..1267
                     /locus_tag="PKM49_07905"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1225..1227,aa:His,seq:gtg)
     gene            1271..1347
                     /locus_tag="PKM49_07910"
     tRNA            1271..1347
                     /locus_tag="PKM49_07910"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1305..1307,aa:Arg,seq:tcg)
     gene            1376..1463
                     /locus_tag="PKM49_07915"
     tRNA            1376..1463
                     /locus_tag="PKM49_07915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1410..1412,aa:Leu,seq:gag)
     gene            1468..1543
                     /locus_tag="PKM49_07920"
     tRNA            1468..1543
                     /locus_tag="PKM49_07920"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1501..1503,aa:Lys,seq:ctt)
     gene            1671..2246
                     /gene="infC"
                     /locus_tag="PKM49_07925"
     CDS             1671..2246
                     /gene="infC"
                     /locus_tag="PKM49_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNY82764.1"
                     /translation="MAKEGKLPPVNEEIRVPKLRVIGKDGQQLGVLTLPDALSRAREA
                     DSDLVLVAPGAQPPVAKIMDFGKYRYEIQKKERESKKKQKKTELKQMKFRPKIDDNDY
                     QTKLKHIRRFIEEGHLVRVTIMFRGREMAFTEKGLEILNQVIADTAHFAKCVSKPKLE
                     GRDMHMSLSPMSEEEMRRLEKQEKGKVEIEG"
     gene            2281..2484
                     /gene="rpmI"
                     /locus_tag="PKM49_07930"
     CDS             2281..2484
                     /gene="rpmI"
                     /locus_tag="PKM49_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNY82765.1"
                     /translation="MAKHKMKTSKTAAKRFRVTKTGKIMRNHSNRSHETGKKPTKIPR
                     RLRTKGQVAKSRVRNILRMLGKA"
     gene            2516..2878
                     /gene="rplT"
                     /locus_tag="PKM49_07935"
     CDS             2516..2878
                     /gene="rplT"
                     /locus_tag="PKM49_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNY82766.1"
                     /translation="MRIKRAVMSKSKRKKFLKAAKGYRGALSRRVRLAKEMYFRSGVY
                     SYVGRKDKKGQYRRMWIIRINAAARLNGMKYSQLIHGLKLAGVNINRKMLSEIAATDL
                     NTFKEYVDIAKASLSKAS"
     gene            2895..2970
                     /locus_tag="PKM49_07940"
     tRNA            2895..2970
                     /locus_tag="PKM49_07940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2928..2930,aa:Pro,seq:cgg)
     gene            3057..4034
                     /locus_tag="PKM49_07945"
     CDS             3057..4034
                     /locus_tag="PKM49_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82767.1"
                     /translation="MGRKTARASRFGRFMLLLFMGVFLGAIFMLLFPQYLVKKPPEGA
                     FLKSAFNGEGLPLSFSIVLWNGLRPPGFYGKQTPPFFPESLLQFAETTLPDLLVFACV
                     PLVEEPTAKGLPERPILPNELDRRFSRYGLYAVKTESDQDTHTTLQTFHLLWSRTKPD
                     VIPKNTASLGLGIFVKKEPSLSLSILEYTREQRKLLVYLPDFSAVRDRSDWEDCLKAL
                     KEEIVAQYAQGKSVLVAGDWRGCLPGAQNCLATMEQIPILWTPEGWSWFYPCYPDLSP
                     FASSPFRDAFSGILASPDLLAREFETFTPQASTSENEPMVFNSAIRVVF"
     assembly_gap    4315..4324
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4367..4975)
                     /locus_tag="PKM49_07950"
     CDS             complement(4367..4975)
                     /locus_tag="PKM49_07950"
                     /inference="COORDINATES: protein motif:HMM:NF013624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HNY82768.1"
                     /translation="MRILLTGFEPFGGDKHNPAQRICEALSESKSEHWDLRTCILPVS
                     FTKAPIALWQAVEEVKPEIAICLGYSAKADCFVIERIALNIEDARIADNDHYRPQDTL
                     VEPEGALALETTLPIRALDTHLRERGIPARISYTAGTFVCNTVFYTLLLEAQTKGYPR
                     LAGFIHVPALPEMALEKREQPFLPLEYMVSGIREILEFLIRG"
     gene            complement(5002..5409)
                     /locus_tag="PKM49_07955"
     CDS             complement(5002..5409)
                     /locus_tag="PKM49_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNY82769.1"
                     /translation="MQEKEQEGDRRSEHRETVKRFVDAFDRQDWDKARECLSRDFWAV
                     WPQTRERFSRDDFIAMNAAYPGRWRLRLRRYEETPEGAVSVVHVTSEAGGAGHYATTF
                     YRFEGDAIRSIEEYWSAEEPAPEWREPFASPIE"
BASE COUNT         1409 a         1456 c         1506 g         1254 t
ORIGIN      
        1 gagagcgcat agagtccgga gaggtgaaac tgacggtcta cctatttcga gcggtcgtgg
       61 ccccagacaa aggaacgtta ttacggcggt acaaagaagc gatgcaagcg ctgaaagagg
      121 tgtcaggtag aagggacata gaagacttga tcgaacaggg tttcggtatc tccttagctc
      181 gaaaccagag atcgagttag aagagctcgc cgaaatcgcg agtaggatct cggaagaaag
      241 gggtgaacag ataatgacgc ttgcgaagag actcatcgat agaggaatga aaaagggtct
      301 ggaaatggga aaagccgatg tgatctggaa acagatgata aaaaagttcc cgaacctcca
      361 agccgcctat ctcgataagt taaaacagtt agacgagatt aggttagaca tactagcttt
      421 ggaattgttg gatatccaaa gcgaggagga gctgaaaaac catttaccga tgtagaaaac
      481 ttcgctgatt aatcgcgagc gcgcagacgc gccttatctt ggcgtgctga gctgtagttc
      541 cgaaaggttt acactgtgaa cgtggcactt tagtgcggca ggtcttgaaa tacaaaacaa
      601 gggttcgcac agagaccgca gagaacacag aggaaaaatc ttggaaagac gctcccaaca
      661 acctaaacca tcccaactaa aaagttgaag ttcgcaggcg ctcccaggcc ttcccagaaa
      721 aacaaaagac ctgccaatct gaagattggc gttcccagac gctcccagaa aaacaaaaga
      781 cctgccaatc tgaagattgg cgttccctaa cgttcccaaa cgttagaagg ccgcaagggc
      841 gctcggaggg ggctttacca gacccgcgct ctatcacttg acgcgcctag gggattatgt
      901 tataattcgc aacgtttacg tcggagcgtg gcgcagctgg aagcgcgcat gccttgggcg
      961 catgaggccg ctggttcaaa tccagtcgct ccgaccaagt aaggcggctg tagctcaatt
     1021 ggtagagcat tacccttcca aggtaagggt tgcgagttcg agtctcgtcg gccgctccag
     1081 caacaggcga gcgcccatag ctcaacagga tagagtattg gatttctaat ccaagggttg
     1141 ggggttcgat tccctctggg cgcgccagga aagccttaca agttaagaac ggtggttata
     1201 gctcagcggg tagagcgtct gactgtggat cagaatgtcg tgggttcaaa tcccactagc
     1261 caccccattt gcgcttgtgg ctcaactgga tagagcatcg gacttcggat ccgatggttg
     1321 ggggttcgaa tccctccagg cgcgccagtt gatggagtag cacgtgggtt tctttgccgg
     1381 ggtggtggaa ttggcagaca cgcatggttg aggtccatgt gggagttttt cccttgcggg
     1441 ttcaagtccc gcctccggca ccaagttggg ccgttagctc aatcggtaga gcaactgact
     1501 cttaatcagt aggttacagg ttcgattcct gtacggctca ccaacaaagt agattgccac
     1561 ccgatggtat ctcaccttcc gacagcggtc gataaggttc atagacgggt attttggtca
     1621 agcgggtggt ccgctttttt tttggtgaat agagaaagga gtggtcagtc atcgcaaagg
     1681 aaggaaaact tcctcctgtc aacgaggaga tacgtgtgcc gaagctgcgt gtgatcggta
     1741 aggacggtca gcaattgggg gttttaacgt tacccgacgc gttatcgaga gcgcgagaag
     1801 cggattcgga cctggtgttg gtcgctcccg gagcccaacc tcccgttgcg aagattatgg
     1861 attttggcaa gtaccggtac gagattcaga agaaggaaag ggagtccaag aagaaacaga
     1921 aaaagacgga actgaagcag atgaagtttc ggcccaagat cgacgataac gactatcaaa
     1981 ccaagttgaa acacatcagg cggttcatcg aagaggggca tttggtcagg gtaacgatca
     2041 tgttccgagg aagagagatg gccttcacag aaaaaggttt ggaaatcttg aatcaagtga
     2101 tcgccgatac agcacatttc gccaaatgcg tttcgaaacc gaagttggaa gggcgagata
     2161 tgcatatgtc actctccccg atgtctgaag aagaaatgcg aaggttggag aaacaggaaa
     2221 aaggaaaggt cgagatagag gggtagtccc gattacccgt atccacaagg aggattggtt
     2281 atggccaaac acaaaatgaa aaccagcaag acggctgcga agcggtttag ggtcacgaaa
     2341 accgggaaga ttatgcggaa ccattcaaac cgctcccacg agaccggaaa gaaaccgacg
     2401 aagatccctc gccgtttacg gacaaaaggc caagtcgcca agagcagagt tcgcaacatt
     2461 ttgcgaatgc tgggaaaagc gtagcacagc gtagaaaaat cgaggaggaa cagaaatgcg
     2521 tattaagcga gcggtgatga gcaaatcaaa gaggaaaaaa ttcctgaaag ccgcaaaggg
     2581 ataccgaggc gctctaagcc gaagggtaag gttggcaaaa gagatgtact tccgttccgg
     2641 agtgtactct tacgtcggcc ggaaagacaa aaaaggtcag tacagaagaa tgtggatcat
     2701 ccgcattaac gccgccgcca ggttgaacgg tatgaaatac agtcaactga ttcacgggct
     2761 gaagttggcc ggagtgaaca tcaaccgaaa gatgttgtcc gaaatcgcgg ccaccgattt
     2821 gaataccttt aaagaatacg tcgatatcgc caaagcatcg ttaagcaaag ccagttaacc
     2881 gtcgtttagc acttcagggc gtagttcagt tggaagaacg ccaggttcgg gacttggagg
     2941 tcgtgagttc aaccctcacc gccctgacca acgggggatt cggaggaaaa cctttccttt
     3001 tgaatcccct tttgtcatag aggctacgcg aatacagaag aacggggtac ttcgctatgg
     3061 gtcgcaagac agcgcgcgcc tctcgattcg ggcgctttat gttgcttttg tttatgggcg
     3121 tctttttggg cgctattttt atgcttctat tcccccaata cttggttaaa aaaccgccgg
     3181 aaggcgcctt tttgaagtct gctttcaacg gagaggggtt gcccctttcc ttttcgatcg
     3241 tgctgtggaa tggccttcgc cctcccggct tttacgggaa gcaaaccccc ccatttttcc
     3301 ctgaatctct ccttcagttc gcagagacca cccttcccga tctgctggtt ttcgcctgcg
     3361 tcccactcgt tgaagagccc actgccaaag gccttccgga acggccaatc ctcccaaacg
     3421 aactcgaccg acggttttct cgatacggcc tctacgcggt gaaaaccgaa tccgaccaag
     3481 atacgcacac gacactccag acctttcacc ttctgtggag tcgaacgaaa ccggacgtga
     3541 tacctaagaa caccgcctca ctcggtttgg gaatcttcgt caagaaagag ccgtccctct
     3601 ctctctccat tctggaatat acgagagaac aaagaaagct gctcgtctat ctgcccgatt
     3661 tttcggcagt tcgtgaccga tccgattggg aagactgcct gaaggctctg aaagaagaga
     3721 tcgtggcgca gtatgcgcag ggaaagagtg tgctcgttgc cggagattgg cgcggctgcc
     3781 ttccgggcgc tcaaaactgc ttggcgacga tggagcagat ccccatactg tggacgcccg
     3841 agggctggag ctggttttac ccgtgctatc cggatctctc cccgtttgcc tcttcacctt
     3901 tccgagacgc gttttccggc atcctcgcct ctcccgacct cctcgcccgc gagttcgaga
     3961 ccttcacgcc ccaggcatcc acgagcgaaa acgaaccgat ggtcttcaac agcgccatcc
     4021 gcgtcgtgtt ttaacgcacg cgttttctgc cgctaaaaac ggctgggaac gccgtgaaaa
     4081 accaaaaaac atttgttcgc acagaggacg cagagagaat acgagattca tgtctgggaa
     4141 cgccctggga acgccgcact tcagtgcggc atggtttaag ggtttttctt tactgggaac
     4201 gccgcacttc agtgcggtat gtgttcacgt tctctccgtg ccctctgtgt tctctgtgcg
     4261 aaatctggtt ttagcccaag aaaagttaag acttgccaat caaaagctta gcgcnnnnnn
     4321 nnnntagcgc tcccaggaat gcgttcccgg gtgctccggt atgggattac ccgcggatta
     4381 gaaattcgag gatctcgcga ataccgctca ccatgtactc caaaggtaaa aagggttgct
     4441 cgcgtttttc taacgccatc tccggtagcg ctgggacgtg gatgaatccg gccaggcgcg
     4501 ggtatccttt ggtctgcgcc tccagcagta gggtgtagaa tacggtgtta cacacgaagg
     4561 tacccgccgt atacgaaatc cgcgcgggga tgccccgttc tcgcaagtgg gtgtccagcg
     4621 cgcggatcgg cagggtcgtt tccaacgcga gggcgccctc cggctcgacc agcgtgtcct
     4681 gaggacgata gtgatcgttg tccgcgatac gcgcgtcttc gatattcaag gcaattcgct
     4741 ctataacaaa gcagtcggcc ttcgcagagt accccaagca gatagctatt tccggcttca
     4801 cttcctcaac cgcttgccat aacgctatcg gcgctttcgt aaacgaaacg ggaaggatgc
     4861 acgttcggag gtcccagtgc tcagatttgc tttcggatag cgcttcacag atgcgttggg
     4921 ccgggttgtg tttgtccccg ccaaagggct cgaaaccggt tagcagtatt ctcatcgtcg
     4981 tcgtcctcct attctctatg tctattctat tggggatgcg aaaggttcgc gccattcggg
     5041 cgccggttct tcggccgacc aatactcctc gatcgaacga atcgcgtctc cctcaaatcg
     5101 gtaaaaggtc gtcgcgtaat gacccgctcc acccgcttcg ctcgtcacgt gcacgacgct
     5161 cacagcccct tccggggtct cctcgtaccg ccgtaatctc aaacgccagc gacccggata
     5221 agccgcgttc atcgcgatga aatcgtctcg tgaaaatcgc tcccgcgtct gaggccacac
     5281 cgcccaaaag tcccgcgaga gacattcccg cgccttatcc caatcctgcc ggtcgaaggc
     5341 atccacgaat cttttaacgg tctctcgatg ctccgagcgc cgatcccctt cctgttcctt
     5401 ctcttgcatt cgtccccctc acccctattc agccccatgc ttcggtgtat cagccttctc
     5461 cacacagtgg aaatggaggt tttttaaatt aaataaccca ttattggctt aattttcgtg
     5521 cataacccaa atgatgtatt tttatggatt tttccgccgc actggaaccc ctgggaacgc
     5581 cttgggaagc cacattcgct tacgccgcga tgaccgtcga aacgctgcga tcccc
//
LOCUS       DAOPXN010000147         5633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14322_length_5633_cov_4.604338, whole genome shotgun sequence.
ACCESSION   DAOPXN010000147 DAOPXN010000000
VERSION     DAOPXN010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14322_length_5633_cov_4.604338"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PKM49_07960"
     CDS             <1..444
                     /locus_tag="PKM49_07960"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82770.1"
                     /translation="NEAYVRITTVFVTREETETSERLYDTFIHYLYYLIAREDFLRLV
                     QLTRTVSVERSERMQTIAEWLKQEGMEKGLEEGMLRGLERGIEVGREQLLWKQISKKF
                     PQIPRTYYEKLKTLTIDQLDNLGLELMDMQNVEELKKHLHAKAGL"
     gene            1059..1928
                     /locus_tag="PKM49_07965"
     CDS             1059..1928
                     /locus_tag="PKM49_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNY82771.1"
                     /translation="MGHLERFIRWMTMQRYSPQTIKEYKREIERFFAFLQRENLPYDA
                     LQMPDIEDYLFSIEASERTRNRALSAVKAFYRFLVSRGILAVDPAKSVKSVRVKRKNP
                     VFLSEGEYLSLFHHLDGEGGFLPERDRVMIALLLTTGIRVSELVRIAFKDISTDRAGR
                     ITLEVLRKGQEMDFIYLNSQVTALFSAYLEKRREISVDSDKVFLSYRLGPLERSAVYR
                     LVRKYLEQCGIEKSKKGPHILRHTFATALMKKDVSLFKIKELMNHKNISTTEKYLHIV
                     EEDLKDVVEQIKL"
     gene            1944..4289
                     /locus_tag="PKM49_07970"
     CDS             1944..4289
                     /locus_tag="PKM49_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNY82772.1"
                     /translation="MYSLRKLEFDKVLAMIADLTFSSYGKDAVLSLQPIEHPQLELSK
                     VSQLIDLFRAYGEPPVGGISDIRREWAKVLVGKILEPGELKRIAVNFDSVARLKRFLD
                     EKSHEYTFLTPFISRLHTLETFVKFAFLAIEDDESISNRASPRLNQIRVDLRRAMRDV
                     RGLLDKLMTGVLKEVVQDTDLLTRDGRYVIPIKASNRHQVAGIVHSSSGSGATYYMEP
                     EALVPMNDAVRNLQSQEREEINRILREMCQMLFQSAREIAETIDALVEWDSLWARARF
                     AIEQDGTIPEISPDGSFSLREAKHPLIGKACVPLSLSLSDELQGIVITGPNTGGKTVS
                     LKTVGLCHLMGLSGIPVCAAMGTRLGRFAHVLVDIGDEQSIEQSLSTFSSHMKNIISI
                     SEYAGPDALILLDELGAGTDPVEGSAIALGILDVFLERGAKVLVSSHMTPLKLYAYQR
                     KELENASVIFDIDDLRPTFQLVVGLAGSSNALIISERLGLNPTVVDRARSFLDRDVHR
                     IDEVLNLLHRQKIEMEEQNSRVRHLRETLERQQQSLEKQLEEIKQKKYGAFLKEIEAM
                     EEELRQIRRYTQTKVAEFRKEKPPAGADIESFNREVQGDITQQLKDLKKNWERSFKQE
                     SREAEIDSLKPGDSILLKDSKLVLRVVEVKGSTLLVEKDGKKLEIPKGFVGQKTEPPA
                     LQPGKEWIHESASAFREDFVGDRLDIRGKTVEEGIDEVEAFISFLLMKNRKSGCVIHG
                     KGTGKLADGLWQRFAKDRRIQSYRIGKPEEGGTGVTIITLS"
     gene            4312..5583
                     /gene="murA"
                     /locus_tag="PKM49_07975"
     CDS             4312..5583
                     /gene="murA"
                     /locus_tag="PKM49_07975"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNY82773.1"
                     /translation="MKSFHLEGGIPLRGQVRISGSKNTTLPMIAASLLTDEPVILKNV
                     PNLEDVRTMVDILKHLGVSVSFRDGMMAISPKNGLGNHLPYESVRKMRASFNVLGPLV
                     MRHGKAHVPLPGGCAIGPRPVDMHLEGMRALGIETTIDYGVVCAERVSKPDSMTFHLR
                     IPSVGTTEQLLSIAVLMEGSEIVLNNVALEPEVQELALFLNRMGARIEGIGTSALRIK
                     GVKRLHGTEYEIIPDRIETGTYLLAALVSQGDVELVGYHPTHNVSLVRKLREMGADVR
                     ESENRIRARYLRRLNGTDFHAEVYPGLPTDLQPQFTVLAAVSEGVSVITDTVFSDRFL
                     HVDELKRLGANCSVRNHAIIVHGVDQLKGAPVKAKDLRGAAALVLAGINAKDRTVVQN
                     IDYIFRGYEEIIPKLRGIGAQCEMVEEELAS"
BASE COUNT         1460 a         1331 c         1574 g         1268 t
ORIGIN      
        1 aatgaagcgt acgtaagaat caccaccgtc ttcgtcacgc gggaagaaac ggaaacaagt
       61 gaaaggcttt acgatacttt tattcactat ctctattatt tgatcgcacg agaggatttc
      121 ttgagactgg tacaactgac gcgaacggta tcggtggaaa ggagtgaaag gatgcaaacg
      181 atagcggaat ggcttaagca agaagggatg gaaaaaggac ttgaggaagg tatgttaaga
      241 ggactcgaaa gagggataga agtcggacgc gagcaacttc tttggaagca gatctccaaa
      301 aaattcccac aaatcccgcg gacatactac gaaaagctga aaacgcttac aatagaccag
      361 cttgacaacc taggcttaga gctgatggat atgcaaaatg tggaagagct gaaaaagcat
      421 ttgcatgcga aagccggact ttagtctgga atgttttgga agaaaaaaca gggtccgtgt
      481 ggagagcacg gaggattctt tgctgggaac tccacacttt tgtgtggcaa ggttatagat
      541 ttttctatgc tggaaacagc gaacttcagt gcggtatggg ttcattgttc tctccgtgcc
      601 cgccgtgcac cccctttttt caagacctgc caacttaaaa gttggcgttt ccagaaacca
      661 agccgaacag aagttcggcc ttcccaaagt ccgctctcag gaaaacaaaa agacctgccg
      721 cgcaaacagc tcgctccacg gtaaatcggg gtttcggtta ccaaaatcaa cacgtaccct
      781 tccaagaccg tagcgaagaa accttgcaat ttcgatgcca gatgagatac aatatatacg
      841 gagtcttcct cggatacgcc gttttctttc ggggctcaac cggggaagga gaacgagcgg
      901 ccgcggctat ctcgtttacg atacgaagtg gcttcggttg aaacaggtat tcggattttt
      961 ggttttcctt ttccggtttt aatcgtcgag cgattaagac cgtcgaaaat cagaaaacgt
     1021 ttctttgtga acagcggtta tgagggggga tcattcggat gggtcatctc gagcgattta
     1081 tcagatggat gacgatgcag cggtattcgc cgcaaacgat caaagagtac aaacgggaga
     1141 tcgagcgttt ttttgctttt cttcagcgtg aaaacttgcc ttatgacgct ttgcagatgc
     1201 cggatataga agactacctt ttttctatcg aggcttccga aagaacgcgt aatcgcgctc
     1261 tgtccgcggt aaaagccttc tatcggtttt tggtgtcacg gggaatcctg gcagtagacc
     1321 ccgcgaaatc ggtgaaatcg gtacgcgtca agcggaagaa cccggttttt ctttccgagg
     1381 gtgagtacct ctctttgttt catcatttgg atggagaggg cggtttcctt ccggaacgcg
     1441 atcgggtgat gatcgccctt ctcctcacaa ccggaatacg ggtgtcggaa ttggtgcgga
     1501 tcgcgttcaa agacatctcg acggatcgcg ccggccggat aaccctcgaa gtcctcagaa
     1561 agggccagga gatggatttc atctacttga actcccaagt gacggcactt ttctcagcct
     1621 atctagaaaa gagacgggaa atctcggtgg acagcgacaa ggttttcctc tcttatcgtt
     1681 tgggccccct cgaacgctcc gccgtctacc ggttggtgcg aaaatacttg gagcaatgcg
     1741 gtatagagaa aagcaagaaa ggcccccata tccttcgcca tactttcgcc accgccttga
     1801 tgaaaaaaga cgtttcgctg tttaagatca aagagctgat gaatcataaa aatatctcta
     1861 cgacggagaa gtatttgcat atcgtcgaag aagacctaaa agacgttgtc gaacagataa
     1921 aactctgagt tggtggcgtg actatgtatt ccttacggaa attggaattc gacaaagtgt
     1981 tggccatgat cgccgacctg acgttttcca gttatggaaa agacgcggtc ctttctctcc
     2041 aaccgatcga gcatccgcag ttagaattgt cgaaggtatc tcaattgatc gatctgtttc
     2101 gagcatacgg agaaccgcct gtgggaggaa tctccgatat ccggagggag tgggcgaagg
     2161 ttctcgtcgg gaaaattctg gaacccggag aactcaaacg cattgccgtc aatttcgatt
     2221 ccgtcgctag actgaaacgt ttcctcgatg aaaagtcgca cgaatacacc ttcctcaccc
     2281 ctttcataag ccgactccac acgctggaaa cgttcgtcaa gttcgctttt ctcgcaatcg
     2341 aggatgacga gtcgatttcg aaccgagcct ctccgaggtt gaatcaaatc cgtgtggact
     2401 taaggcgtgc tatgcgcgac gttcgggggc ttttggacaa gctgatgacc ggcgtgttga
     2461 aagaagtggt ccaggacacc gaccttctca cgagagacgg tcgttacgtc ataccgatca
     2521 aagcctcgaa ccgccatcag gtcgctggga tcgtgcattc atcctcggga agcggcgcca
     2581 cttactatat ggaacccgaa gccctggttc ctatgaacga cgccgtccga aacctgcaat
     2641 ctcaggagcg cgaggagatc aatcggattc tcagagaaat gtgccagatg ctctttcaga
     2701 gcgcgcgaga gatcgcagaa acgatcgacg cgttggtgga atgggactcc ttatgggcgc
     2761 gggcgcggtt cgcgatcgaa caagacggga cgattccgga aatctctccg gatggctcct
     2821 tttccttgag agaagcgaag catcccctga ttggaaaagc ttgcgtgcca ctctcgcttt
     2881 ctctatccga cgaattgcag ggaatcgtga tcacgggtcc gaatacgggg gggaagaccg
     2941 tctcgctcaa gacagtagga ctctgccatc ttatgggttt atccggaatt ccggtttgcg
     3001 ctgcgatggg cacgcgactc gggcgttttg ctcacgtttt ggtggacatc ggagacgaac
     3061 agtcgatcga gcagagtctg agcacctttt cctcccatat gaagaatatc atctcgatca
     3121 gtgaatacgc cggccccgac gcattgatcc ttttggacga actcggcgcg ggtaccgatc
     3181 ccgtcgaggg ttctgcgatt gcgctgggga tcctcgatgt ctttctggaa cgcggggcca
     3241 aagtgctggt gagctcccat atgaccccgc tcaaattgta cgcctatcag aggaaagaac
     3301 tcgaaaacgc gtcggtgatc tttgacatag acgatctcag acccaccttc caattggtgg
     3361 tggggttggc gggcagctcc aatgccttga tcatctccga gcgtttggga ttgaacccaa
     3421 ccgtcgtcga tcgggcgcgt agctttttag atcgggatgt ccaccgaatc gacgaagttt
     3481 tgaacctcct tcaccgtcaa aaaatagaga tggaggagca aaacagccgg gttcgtcatc
     3541 tccgggagac tctggaaagg cagcagcaat ctttggaaaa acagctcgag gagattaaac
     3601 agaagaaata cggcgccttt ctcaaagaga tagaagcgat ggaggaagag ttgcgccaaa
     3661 tcaggcggta cacccagacg aaggtggccg aattcaggaa agaaaaaccg ccggccggcg
     3721 cggatattga gtctttcaac cgcgaggtac aaggggatat cacccaacag ctaaaagatc
     3781 ttaaaaagaa ctgggagcgg tcgttcaaac aagagagtcg agaggcggag atcgatagtc
     3841 tgaagccggg ggacagcatt ctgctgaaag acagcaagct agttttacgg gttgtcgagg
     3901 tcaaaggatc gacgcttcta gttgaaaaag acgggaaaaa gctggaaatc ccgaagggct
     3961 ttgtcggtca aaagaccgaa cctccggctt tacagcccgg caaggaatgg attcacgaaa
     4021 gcgcaagcgc attccgcgaa gacttcgtcg gagaccggct cgatattcgc gggaagacgg
     4081 tggaagaagg gatcgacgag gtagaggcgt tcatctcatt tttgttgatg aaaaaccgaa
     4141 agagcggctg tgtgattcac ggcaaaggga cggggaagtt ggcggacggt ttgtggcaaa
     4201 ggttcgccaa agatcggaga atacagtctt atcgaatagg aaagcccgaa gagggcggaa
     4261 cgggagtcac gattattacc ttatcttaat ctactatgag ggggaattct aatgaaaagt
     4321 tttcacttgg aaggaggcat tccgctacga ggtcaagtca gaattagcgg ttccaagaac
     4381 acaacgttgc caatgatcgc ggcctctttg ttaacagatg agccggttat cttaaagaac
     4441 gtaccgaatc tcgaagatgt gagaacgatg gtcgacattc tcaagcattt gggtgtgagc
     4501 gtttctttcc gagacgggat gatggccatc agcccgaaga acgggctagg aaatcatttg
     4561 ccctatgagt ctgtacggaa gatgcgcgct tcgttcaatg tgttaggccc gcttgtgatg
     4621 cgccacggga aagcccacgt gccgcttccg ggggggtgtg cgatcggtcc aaggcccgtt
     4681 gatatgcatc tggaagggat gcgggctttg gggatagaaa ccacgatcga ctacggcgta
     4741 gtctgcgccg aacgcgtgag taagccggac tctatgacct ttcatctaag aattccgtcg
     4801 gtcgggacga ccgaacagct cctgtcgatc gctgtcctta tggaagggtc cgagattgtg
     4861 ctgaacaacg tcgcattgga gccggaagta caggagctgg cgctgttttt aaaccgtatg
     4921 ggcgcgcgga tcgaagggat cggcacctcc gccctccgca tcaagggcgt gaagcgactg
     4981 cacggtacag aatacgagat catcccggat cgtatcgaaa caggcaccta tctattggcc
     5041 gccctggtct ctcagggaga cgtcgaattg gtcggatatc atccgaccca caatgtcagc
     5101 cttgttcgga agcttcggga gatgggcgct gacgtccggg aatcggagaa ccgaatacgt
     5161 gcccgctatc ttcgacggtt aaacggtacg gattttcacg cggaggtata cccgggtctc
     5221 cccactgatc tccaacctca gttcactgtg ttggcggctg tttccgaagg cgtttccgtc
     5281 atcacggaca cggtcttttc cgatcgattc ctacacgtgg acgaattgaa acggttggga
     5341 gcgaactgct cagtgcgtaa tcacgcgatc atcgtacacg gcgtggacca attgaaaggc
     5401 gcgccggtca aggcaaaaga cttgcggggg gccgccgcgc tcgtcctcgc ggggatcaac
     5461 gccaaagacc ggaccgtagt ccaaaacatc gattacattt tccggggcta tgaggagatc
     5521 ataccgaaat tgagaggaat aggagctcaa tgcgagatgg tagaagagga gttggcgtct
     5581 tgaaaacgat cgtgatagca ggagcaggtt cgaaaacggg aaagacaacc gtg
//
LOCUS       DAOPXN010000148         5568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14544_length_5568_cov_4.919100, whole genome shotgun sequence.
ACCESSION   DAOPXN010000148 DAOPXN010000000
VERSION     DAOPXN010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14544_length_5568_cov_4.919100"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            384..1205
                     /locus_tag="PKM49_07980"
     CDS             384..1205
                     /locus_tag="PKM49_07980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY82774.1"
                     /translation="MSYRLFSFEELSLSEQTDCFNRSFEDYVIPIHLTPSQLEKRYER
                     EKLRPEVSLACEIDGQLAGMCLNGRNGALAYCGGFGIFQPFRGRGFGRRFFNDVISFQ
                     REKGVREYQLEVICENTAAYLLYKGLGFKEFDQVYVLEGHFPGNGNTAGKEPDASVGY
                     LPMLDACSFDRSERVWGCSPYVLSDMPNLYSTLLDAPGLVGGVAYTKQGDALCIRDLR
                     VKPSEGETAYACLTRAFSGRKATFYFAHQSQPIHRLLLDNGWRVLHRQFALRLGL"
     gene            complement(1256..2905)
                     /locus_tag="PKM49_07985"
     CDS             complement(1256..2905)
                     /locus_tag="PKM49_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNY82775.1"
                     /translation="MKKILCGAIGSCVHVAGLYRFTQLAEKEGYLTTYLGGAVPIQKM
                     IDAVVEVQPDIVALSYRLSAEALEKLLEDFFADLEKVDRRKRVFLFGGTIETAAIARS
                     FPQFSRLFDGSEDEEEVVLFLRNQTAEAEEQRIPPQRLGERIVWKRPYPLIRHHIGLP
                     TLEETGEEVKILADSRLLDIISLAPDQNAQQSFFRPKEIDPKQDGAGGAPFRSEGDFK
                     RLYQASRRGNHPLMRCYSGTRDLIPFSQVLIDTIHNAWAAVPITWYSALDRRSDRPLL
                     EAIRENQQAIRYNAERQVPVEVNEAHQWALRFAHDAMDVATSYIATWSAYRLGVQEYV
                     MQFMLCTPPELSPKMDLAKALAKWEMVRPLVEESERRGSPFRIYKMIRTGLLSYKTDT
                     DLSKGQLTASMFYGAFLQPDIVHVVAYCEAIRRATSVEILESVKMVKQAINMAYRGLP
                     DLSADSELEARKDLLIAEASVILDAIRKLDRTIEDPLSTPEILEQAILTGILDAPGLS
                     KGPAKADVKTGIVNGANESLDENGSVLPEKDRIGRLGARLV"
     gene            complement(2905..3990)
                     /locus_tag="PKM49_07990"
     CDS             complement(2905..3990)
                     /locus_tag="PKM49_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HNY82776.1"
                     /translation="MKICVIGAGNGGQALAGYLALRGEKVSLFNRSRKRISPFMKSKN
                     IRLEGAVNDIAHLELVTTNLEEAVKGRELIMVVVPAFAHRSIAEKLIPYLEDGQVILL
                     NPGRTFGALEFSMIISHAKTNKSVTVAEAQTFVFASRASNPGVVHIFRIKNAVPVAAL
                     PSSNNPIVAAMLEKVMPEFAVERDVLYTSINNIGAVFHPAALILNAGWVESTSGKFEF
                     YLDGITPSVARVLEEIDAERCRLAEALGVEPLTSKAWLDYAYDVYGANLYEAIHSNTG
                     YQGIMAPPSMQNRYIYEDVPMSLVPLASMAKEFSLPTPTIDAVVQLASTIMGIDFVKE
                     GRTVEKFHLNGKTKEEIIEIVSKGLIL"
     gene            complement(4279..4366)
                     /locus_tag="PKM49_07995"
     tRNA            complement(4279..4366)
                     /locus_tag="PKM49_07995"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4330..4332),aa:Ser,seq:tga)
     gene            complement(4370..4597)
                     /gene="rpmE"
                     /locus_tag="PKM49_08000"
     CDS             complement(4370..4597)
                     /gene="rpmE"
                     /locus_tag="PKM49_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNY82777.1"
                     /translation="MREKIHPAMHRVTALCACGAKYDILTTNENVKLDVCSRCHPFFQ
                     GKGDSLIIDTEGRIDKFRKRYGDKIGGAGTR"
     gene            complement(4666..>5568)
                     /locus_tag="PKM49_08005"
     CDS             complement(4666..>5568)
                     /locus_tag="PKM49_08005"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNY82778.1"
                     /translation="AVFSTVRGFVKDIKQEENGKTKVYVEDYEESIHDYELPSTLKAK
                     VKIGDKLLPGSSISTGAVRPRELMQELDVDTTFKYLLREIKRVYSEQGVDIHNKHIEI
                     IIRQMLGKVEVVEPGDTDYLVGDLVSFQQVKKTNEIILKSNAKVNENRKRVLDKTVKK
                     RILISREGEMIELAAEGSVVTEELLKELVKEGIKVVEIAEEEQTKEYQINTKEPVKYR
                     RKLLRITKASLERIGWLSAASFQQTTQVLTENALKGSVDELKGLKENVIIGQLVPAGT
                     GMDWYSAIDYEEAFHYEQKTAPGA"
BASE COUNT         1142 a         1531 c         1435 g         1460 t
ORIGIN      
        1 gcgttgtttt ttcttatttt ttcccttttt ctccaaaacc ggtcgaaagt cgggataata
       61 tcggggtgca acaaaaaata gaaagaggag gagatccgtc ttttgtcctt tttacgttgc
      121 gatgcctgtg tgggtcatcg cgtggtaagt ggtttttacg accccgcgat ccgtcttttg
      181 tttttccaaa gtttcgcgat gggtcgggaa aaaagcgtat cgcgccgtcg cgaaaagaag
      241 aagcgcttac gccgcgatgg cgcttccaga aagtctggcg ttcccagaag tgatgtcggt
      301 tgcttttttc ttatccttag cctataataa cgggtatcag cgtctcaacc gcgtagattt
      361 gtatagcccg aagaggtgtg tctatgtctt atcgcttgtt ttcattcgag gaactctcgc
      421 tctcggagca aacggactgt tttaaccgta gtttcgaaga ctacgtgatc ccgattcatc
      481 tcacgccttc ccaattggag aagcggtacg agagggaaaa gctacggccg gaggtttccc
      541 tggcctgcga aatcgacggg cagctggcgg ggatgtgttt gaacggacga aacggcgcgc
      601 tcgcctattg tggcgggttt ggaatattcc agccctttcg cggccgaggt tttgggcgtc
      661 gcttttttaa cgacgtcatc tctttccaaa gagagaaggg cgtccgtgaa taccaattgg
      721 aagtgatctg cgaaaacacc gccgcgtatt tgttgtacaa aggcttgggg ttcaaggagt
      781 tcgatcaggt atacgtttta gagggccatt ttccggggaa cggtaatacc gccggaaagg
      841 agccggacgc ttccgttggc tatcttccta tgcttgacgc ttgttctttt gaccgctccg
      901 aacgcgtttg gggttgttcg ccctacgttt tgtccgatat gcccaacctt tattctacac
      961 tgttggatgc cccggggctc gtcggtggcg tcgcatacac gaaacaggga gatgcgttat
     1021 gcatccggga tttgagggtc aaaccctccg agggagaaac cgcctacgcg tgccttaccc
     1081 gcgccttcag cggccgaaaa gcaacctttt atttcgccca tcaaagtcag ccgattcatc
     1141 gattgctcct cgacaacggt tggcgtgtcc ttcaccggca gttcgccttg agactcggcc
     1201 tttaattccc cgcgagccct tccaatgggg gggttcacac cctcatccgt aaccgttaaa
     1261 ccagacgcgc cccaagccgg ccgattcggt ctttttccgg caggaccgaa ccgttttcat
     1321 ccagcgattc gtttgccccg ttcacgattc cggttttaac atccgctttc gccgggcctt
     1381 tgctgagccc gggtgcgtct aaaatgccgg ttagtatcgc ctgttcgaga atctcgggcg
     1441 tactcaatgg gtcttctatc gtacggtcca gctttcggat cgcatccaag attacggatg
     1501 cctccgcgat caataggtct tttctcgcct cgagttcaga atctgccgat aagtccggta
     1561 accctcggta ggccatatta atcgcttgtt tgaccatttt tacgctttcg agtatctcca
     1621 cgctcgtcgc ccggcggatc gcctcacaat aggcaacgac gtgcacgatg tccggctgca
     1681 aaaaggcgcc atagaacatc gatgcggtca gctgaccctt cgagaggtcg gtgtctgttt
     1741 tgtaagaaag gagccccgtc cgtatcatct tgtaaatccg gaagggggac cctcgtctct
     1801 cgctttcttc gacgaggggg cggaccatct cccactttgc caacgccttc gccaggtcca
     1861 tcttcggaga gagctccgga ggcgtgcata gcataaactg catcacatac tcttgcacac
     1921 ccaagcgata ggcgctccag gtggcgatgt aggatgtggc cacgtccatc gcatcgtggg
     1981 caaaacgtag ggcccattga tgggcttcgt tgacctctac cggaacctgt cgctccgcgt
     2041 tgtatcgaat ggcctgctgg ttttctcgta tcgcttccaa caagggcctg tccgaacgtc
     2101 ggtcgagcgc ggagtaccac gtgatgggca ccgcggccca cgcgttgtgg atggtatcga
     2161 tcaatacctg agagaacggg atcaggtccc gtgtccctga atagcagcgc atcaacgggt
     2221 gatttcctcg ccgagaggcc tggtagaggc gcttgaagtc tccttcactg cgaaagggcg
     2281 caccccccgc gccatcttgt ttaggatcga tttcttttgg cctgaagaag gactgttgcg
     2341 cgttttggtc gggcgccaga gaaatgatgt ccagcaagcg cgaatccgcg aggattttca
     2401 cctcttctcc ggtctcttcc aatgtgggca agccgatgtg gtggcggatg agcgggtaag
     2461 gtcgtttcca gacgatccgc tcgcccaacc gttggggagg tatccgttgc tcctcggctt
     2521 cggcggtttg gtttctaagg aaaagaacga cttcctcctc atcctcgctg ccgtcaaaga
     2581 gtcgcgagaa ttgcggaaaa gaccgcgcga tcgccgccgt ttctatcgtt ccgccgaaca
     2641 agaacactcg cttacgccga tcgaccttct cgaggtcggc gaaaaaatct tccaataact
     2701 tctccaacgc ttccgcgctc agtcgataac tgagcgcgac gatatccggt tgcacttcga
     2761 cgaccgcgtc gatcatcttt tggatgggaa cggctccgcc taaataggtg gtgaggtacc
     2821 cctctttttc cgccagttgg gtgaaccggt acaagcccgc aacgtgtacg cagcttccga
     2881 tcgctccaca cagtatcttt ttcatcaaag aatcaaccct ttcgacacga tctctatgat
     2941 ctcctctttg gtcttcccgt taaggtggaa tttctcgacg gtgcggcctt ctttgacgaa
     3001 atcgatgccc atgatcgtgg aggccaattg gacgaccgcg tcgatcgtgg gggtggggag
     3061 agagaactct ttggccatcg aagccagcgg aaccaggctc atcgggacat cttcgtaaat
     3121 atagcgattc tgcatgctcg ggggagccat gatcccttga tagccggtat tgctgtgtat
     3181 ggcttcgtag aggttcgcgc catagacatc gtacgcgtag tccaaccacg ctttcgaggt
     3241 caagggctcc accccaagcg cttcggccag acgacagcgt tcggcgtcga tctcttcgag
     3301 taccctggcg accgacgggg tgatcccgtc gagataaaac tcgaattttc ccgaagtgga
     3361 ctctacccat ccggcgttga ggatcagcgc ggccgggtga aagacggcgc cgatgttgtt
     3421 gatactggtg taaaggacgt cacgctcgac cgcgaactcg ggcatcactt tctccaacat
     3481 cgccgcgacg atcgggttgt tcgaggaggg gagggcggcg accggcaccg cgtttttgat
     3541 cctgaatata tggacgacgc cgggatttga cgcccgggaa gcgaaaacga aggtttgcgc
     3601 ttccgccacg gtgaccgatt tgttcgtctt cgcatgtgag atgatcatac tgaactctaa
     3661 agcgccgaag gttctcccgg ggttcaggag gatcacttgg ccgtcttcca aataggggat
     3721 cagcttttcc gctatgcttc gatgcgcaaa ggcgggaact acgaccatga tgagttctct
     3781 gcctttcacc gcctcttcca aattcgtcgt aaccaattcc aggtgggcga tgtcgttgac
     3841 tgccccttcc aaccggatat tcttactttt cataaacggc gaaatcctct tcctgctcct
     3901 gttgaaaaga gagacctttt ctcctcgcaa ggcaaggtac ccggccaaag cctggccgcc
     3961 gtttcctgcg cctatcacgc aaattttcat cttaaacacc tcctacagag tatatacaat
     4021 gtatctaata aaaacgcctt tccggtcctt tcagctgtgt gtggaggaaa aggaatccgc
     4081 cataggctgc ctttcctctg gactggtagg gctaattctg ggtgggccgt cgtaaagggc
     4141 atcgacgggt ctggagtcgg aaggtcccct cgttcgagca ggacgagcgt agaatactag
     4201 cggatagaag gagaataaaa aacaaacaaa aaaggagtcg caaaaaaggt agtgcgatcc
     4261 ttttctagac tccttttttg gcggagacgg tgggattcga acccacacgg agcgtagctc
     4321 caccggtttt caagaccggc cccttagcca gttcggacac gtctcccgac tatcttgtcc
     4381 ccgctccgcc gatcttgtcc ccgtatcgct tcctgaattt gtcgattctt ccttccgtgt
     4441 ctatgatcag ggagtcgccc ttcccttgga aaaaaggatg gcatcggcta caaacgtcga
     4501 gcttcacgtt ttcgttcgtg gtcaatatgt catacttcgc tccacaagcg cacagggccg
     4561 ttacacgatg catcgccgga tggatctttt ctctcattga acctcacctc taattatccg
     4621 aaaaattctg cctattatat catagtacgg gggatgagac caaccctacg ccccgggagc
     4681 cgttttctgc tcgtaatgaa acgcctcttc gtagtctatg gccgagtacc agtccattcc
     4741 ggttccggct ggaacgagtt ggccgataat cacgttttct ttcaggcctt tcagctcgtc
     4801 aacacttcct ttcagagcgt tttcagtcaa gacttgcgtc gtttgttgga acgaggcggc
     4861 gctcaaccag ccaattcgct ctagcgacgc tttggtgatc cttaaaagtt ttcttcgata
     4921 tttcaccggc tctttggtgt tgatctgata ctctttcgtc tgctcctctt cggcgatctc
     4981 gaccactttg atcccttctt ttaccaattc tttaaggagc tcctcggtaa ccacggaccc
     5041 ctccgccgca agctcgatca tttcaccctc acgcgaaatc aggatacgtt ttttcacggt
     5101 tttgtccaaa acacgtttcc tattttcgtt caccttcgcg ttggatttca ggatgatctc
     5161 gttcgttttc ttgacctgtt ggaatgaaac gaggtccccc accaggtaat cggtgtctcc
     5221 cggctccacg acttcgacct ttcctagcat ctgccggatg atgatctcga tgtgcttgtt
     5281 gtggatatcg acgccttgct cggagtaaac tcgctttatt tctctcaaga ggtacttgaa
     5341 ggtcgtgtcg acatccagct cttgcatcag ttcccgaggt ctcaccgcac cagttgagat
     5401 agaactcccc ggcaacagtt tgtcccctat cttgactttc gctttcaggg tactcggaag
     5461 ctcataatcg tggatgcttt cctcgtagtc ttcgacgtag acttttgttt tcccgttttc
     5521 ttcttgtttg atatctttta cgaatccacg aaccgtactg aatacggc
//
LOCUS       DAOPXN010000149         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14563_length_5563_cov_5.085694, whole genome shotgun sequence.
ACCESSION   DAOPXN010000149 DAOPXN010000000
VERSION     DAOPXN010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14563_length_5563_cov_5.085694"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PKM49_08010"
     CDS             <1..480
                     /locus_tag="PKM49_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82779.1"
                     /translation="QAVGAIGNPINTVSVPKYFSFFTNTPEMGFRTGFGMKLPFAAVG
                     MANISVREETVFAGFEGWVPIPVTPFGSANGKYRFNGLTFHTGAYLTNDPSLFQCIAG
                     FEVDFGFQYWVNMRIGIDLVLQKDKIGPKFSLNFSDLAFGREGSARENPLTFSSLLP"
     gene            523..1605
                     /gene="disA"
                     /locus_tag="PKM49_08015"
     CDS             523..1605
                     /gene="disA"
                     /locus_tag="PKM49_08015"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HNY82780.1"
                     /translation="MAKIELFKEIMQMIAPGTELRNGIDNILSAKSGALIVLMSEEDI
                     KRWQTLVQPGFYVNCEFSAQRLFELSKMDGAVIITEDAKRILYANVQLAPNSDIPTFE
                     TGMRHRNAERFAKQTGRPAVAISRRRSIISVYWGGFHYVLNDYNLLFTKLNQGLKALE
                     KYSDSFTKALDALEVLEVEGLVTLFDICKTIEKAVRAQMVANEIEPYILETGVDGRLI
                     QMQKDEMLREIIETIELLVLDYYNTETALEGSKLKEVFSRFFQMTEKELGDYNRLASV
                     LGYKPDTTVTEYTVFPKGFRLLRKIPKIPMSVIYNLVRRFSNLPGIESATLEDLKKVD
                     GIGEARARAIILGFANLRKKINLSER"
     gene            1616..2044
                     /gene="umuD"
                     /locus_tag="PKM49_08020"
     CDS             1616..2044
                     /gene="umuD"
                     /locus_tag="PKM49_08020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNY82781.1"
                     /translation="MVKVTEVYFPDLYTSYRIPMAGSHISAGFPSPADDFIEVGLDLN
                     TELIKNPAATYFVRVSGDSMVDAGIHNGDILIVDRSLEVREGKIVIAILDGELVVKRL
                     RRIKGQLYLVPENKSFRPIPIHEESEFLIWGIVTNVIHSV"
     gene            complement(2173..3660)
                     /locus_tag="PKM49_08025"
     CDS             complement(2173..3660)
                     /locus_tag="PKM49_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HNY82782.1"
                     /translation="MGQDDEKRASSFWGSASIYQIFPDRFAIGQGVTAEDKYREGRYP
                     YPEQSIARWTDAPYRNPKGGHQHRFWGGDLPGITEKLDEIHALGCDTLYLTPIFQAIT
                     NHKYDTVDYFKIDPAFGTIDDFRELCAEVHKRGMRLILDGVFNHASRQCLWLSEGRDE
                     FFYPLGNGEYRCWENIPHLVEINLENPAAQEVLFYGKNSVLKYWLREGADGWRIDCAH
                     DLGPRLLESISHAMRAEFPDALLVGEVWNYPAGWKNTGMDGIMNYTFQSVVYDFLRGI
                     LSAGRTGAFLADMAKDTGLAFLSRCWNMCSSHDVERLGTILPKEELVRLAIVLQFAYP
                     GTPFIYYGEERGMMGGPDPENRGAVNWEEPDRYPERRALITRLNRLRKEKRALTAGLF
                     ELVTVSDERVLGFKRRTADVAEQLIVLINPTPHPIETRVYLKDGWVMGGTRILDLDTG
                     EAYRSSMERFVATLAPYQYRLLEPQVRYHSERYTPYKRLEGLTDR"
     gene            complement(3667..4005)
                     /locus_tag="PKM49_08030"
     CDS             complement(3667..4005)
                     /locus_tag="PKM49_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNY82783.1"
                     /translation="MKTLNWYEEMGFAITVSDKNGVILYMNKKACDSNEALGGSMVGK
                     NLFDCHPAKSQGRVRELYEKELTEAYTVEKKGRKKLVYHAPWYENGEFAGIVELSIPL
                     PLEMSHYVRE"
     gene            complement(4012..5199)
                     /locus_tag="PKM49_08035"
     CDS             complement(4012..5199)
                     /locus_tag="PKM49_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HNY82784.1"
                     /translation="MSGPLTGIRVVDLTRVLAGPFCAMVLADLGAEVIKIERPITGDD
                     SRAYPPFQNQTSAYFVSVNRGKKSVTMNLKDPDAKEVLLRLVEKSDIFIENFKPGVAD
                     RLGLSYEELEKVNPRIIYGSSCGYGQTGPYRNKASYDIIIQGMSGMMSITGPDADHPT
                     KVGSSIADIVTGLYLCISLLAALNAREKTGKGQKIDVAMLDCMVSILENALARYTCTG
                     IVPVPVGNVHPSIAPFFTAKTKDYWINIAVGNQDLWKRFCNLTGLSFLANDERFATNE
                     RRVEHWAVLKPILEAALLEKTRDEWLALFEQESIPSGPIYTMADVVNDAQIAAREMIL
                     NVFHTQGGEFKVPGIPMKFSGTVPSDPCTAPVLGEHNQEIYLGLLGLSCEEYMKLKEK
                     GAI"
     gene            complement(5230..>5563)
                     /locus_tag="PKM49_08040"
     CDS             complement(5230..>5563)
                     /locus_tag="PKM49_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HNY82785.1"
                     /translation="DQWELTNRARAYDNHVYWVATNAVGKDASGSHFFGSSMIIHPSG
                     QKLAQARNGNEYIWAALEPDPIKTIYPGSSIPQWFDHIEDRNIRSYDGILENGRAAFD
                     PAKRIPSE"
BASE COUNT         1267 a         1515 c         1418 g         1363 t
ORIGIN      
        1 caggctgtgg gggccatagg aaacccgatc aatacggtga gtgtgcccaa gtatttctct
       61 ttcttcacca acacgccaga gatgggtttt agaacgggat tcgggatgaa gcttcccttc
      121 gccgccgtcg gaatggcgaa catcagcgtg agagaagaaa ccgtcttcgc tggattcgaa
      181 ggctgggtgc cgattccggt tactcctttt gggtcagcga acgggaaata ccgttttaac
      241 ggtctcactt tccataccgg cgcctatttg acgaacgatc cgagcctctt tcaatgtata
      301 gcgggctttg aagtggattt cgggttccaa tattgggtca atatgaggat aggaatcgat
      361 ctcgttttac aaaaagataa aattggccca aagttttcgc ttaacttctc tgaccttgct
      421 tttggcagag agggctctgc gcgcgaaaat ccattgactt tttcctcttt gctaccgtga
      481 caaacaggat cagaagactc agaatatgga taggagaaca taatggccaa aatagagctg
      541 tttaaagaaa ttatgcagat gatcgcaccc ggaacggaac tgcgtaatgg gatcgataac
      601 atcctcagcg ccaaaagcgg agcgctgatc gtattgatga gcgaagagga catcaagcga
      661 tggcaaactc tcgttcaacc cggtttctat gtgaactgcg agttttctgc ccaacgtcta
      721 ttcgagctct ccaaaatgga cggcgcggtg atcatcaccg aagacgccaa gaggattctc
      781 tacgccaacg tccagttagc cccgaactcc gatatcccca ctttcgaaac ggggatgcgt
      841 catcgcaacg cggagaggtt tgcaaaacaa accgggaggc cggcagttgc catctcccgt
      901 agaaggtcga ttattagcgt ttactggggc gggtttcatt atgtcttaaa cgactacaac
      961 cttctcttca cgaaattgaa tcaaggattg aaagcgttgg aaaaatacag cgacagtttc
     1021 acaaaagcgc tagatgcttt ggaagtttta gaggtcgaag gcctcgtgac gctcttcgat
     1081 atctgcaaga ccatcgaaaa agcggttcgc gcgcagatgg tcgccaacga aatcgagccc
     1141 tatatcttgg aaacgggggt cgacggccga ttgatacaga tgcagaagga cgagatgctc
     1201 cgcgagatca tcgaaacgat cgaactgctg gtgttggatt attacaacac cgaaacggcc
     1261 ctagaagggt cgaaactcaa agaggtcttt tcgcggtttt tccagatgac cgagaaagag
     1321 ttgggagatt acaatcgtct cgcatcggtt ttaggctata agcccgacac aacggtcacg
     1381 gagtatacgg tatttccaaa aggatttcgt ttgctccgaa aaatccctaa aattccgatg
     1441 tctgtgattt acaacctcgt tcggcggttc tccaaccttc cgggaatcga aagcgcgacc
     1501 ctcgaagact tgaaaaaagt ggatggcatc ggcgaagcga gggcccgcgc gatcatcctg
     1561 ggcttcgcga acctccggaa aaaaatcaac ctctccgaga gatgaggtga gcagaatggt
     1621 aaaagttacg gaagtgtact ttcctgacct gtatacctct taccgcattc cgatggcggg
     1681 aagccatatc tcggcgggat ttccttctcc tgcggacgac ttcatcgaag tcggtctgga
     1741 cctgaacacc gagttgataa aaaatccggc cgcgacttac ttcgtgcgcg tgagcggcga
     1801 ttcgatggta gacgcgggga ttcacaacgg cgatattctc attgtggacc gatcgctcga
     1861 agtgagagaa ggcaagatcg tcatcgcaat attggacgga gagttggtgg tgaagcgact
     1921 ccggcgaatc aaaggccagc tctacctcgt tcccgaaaat aagagctttc gcccgattcc
     1981 gatccacgag gaatcggaat ttctcatctg gggcatcgtc accaacgtga tccattccgt
     2041 ttagaaagtg tcattttagt aagagccatc gaccgggcgc accgttgctt cgtaatttcg
     2101 aatgtcgtca taaaaagcct cgcggagcac cccggcaagc ggtttgaacc gaaccgagca
     2161 tttttagcgg agttaccggt ctgtcagtcc ttccaggcgt ttataagggg tataccgctc
     2221 gctgtgatag cgaacctgcg gttcgagcag tcgatactga tacggcgcca aggtcgcaac
     2281 gaaacgctcc atagagcttc gataggcttc ccccgtgtcg aggtccagaa tccgcgtccc
     2341 gcccataacc caaccatctt tgaggtaaac cctcgtctct atcggatgcg gtgtcgggtt
     2401 gatcaatacg atcagctgtt cggcgacatc ggccgttcgc cttttaaaac cgagtacgcg
     2461 ctcgtcggag acggtcacca actcaaaaag gcccgccgtt agcgcgcgtt tctctttccg
     2521 caaacggttg agtcgggtga tcaaggctcg acgttcgggg tatcgatctg gttcttccca
     2581 gttcaccgcg ccacgattct cgggatccgg cccccccatc atccctcgct cttctccata
     2641 atagatgaaa ggcgttccgg ggtaggcaaa ctggagcacg atcgccagtc gaacgagctc
     2701 ttctttcggc aggatcgtgc cgagcctctc tacatcgtgc gaagaacaca tattccagca
     2761 acgggataaa aacgccaacc cggtgtcttt cgccatatcc gctaaaaaag cgcccgttcg
     2821 tccagcgctc agaatccctc tcaagaagtc gtaaacaacg ctctggaagg tgtagttcat
     2881 gatcccgtcc attccagtgt tcttccaacc tgcaggatag ttccacacct ctccgacgag
     2941 caaggcatcc gggaactccg ctctcatcgc gtgggagata ctttccagca gtcgggggcc
     3001 caagtcgtgc gcgcaatcga ttctccaccc gtccgctccc tcacgcagcc agtatttcaa
     3061 cacgctgttc tttccataaa aaagaacctc ttgcgccgcg gggttctcga ggttgatttc
     3121 taccagatgt ggaatattct cccaacagcg gtactcaccg tttccgaggg gataaaaaaa
     3181 ctcgtcgcgg ccttctgaaa gccaaaggca ttggcggctt gcgtgattga agacgccgtc
     3241 gagaatcaag cgcattcctc gtttatggac ttcagcgcat agttctcgaa aatcgtcgat
     3301 cgtcccaaag gccgggtcga tcttaaaata gtcgaccgta tcgtacttgt gattggtgat
     3361 cgcttggaag atcggtgtca agtataaggt atcgcatccg agcgcgtgga tctcatcgag
     3421 cttttccgtg atccccggca ggtctcctcc ccaaaaccgg tgttgatggc cgcctttagg
     3481 attcctgtac ggcgcgtccg tccacctggc tatgctctgt tccgggtagg ggtaacgtcc
     3541 ctcccgatac ttgtcctcgg cggtgacccc ctgtccgatg gcgaagcgat ccggaaagat
     3601 ttgatagatc gaagcgctcc cccaaaacga ggacgcgcgt ttctcgtcgt cctgccccat
     3661 ggctcgctac tctcgcacgt aatgggacat ttcgagtgga agcgggatag acagttcgac
     3721 aataccggcg aactccccgt tttcatacca gggcgcgtga tagaccagtt tttttcgccc
     3781 tttcttttcg actgtgtacg cctctgtcaa ctccttttca tacagctctc ggaccctacc
     3841 ctgggacttc gccggatgac aatcgaacag gtttttcccc accatactgc cgcccagcgc
     3901 ctcattgctg tcacacgctt tcttattcat gtacaggatg actccattct tgtcactgac
     3961 cgtaatcgcg aatcccattt cctcatacca gttcaaggtt ttcatagcag gttatatcgc
     4021 tcctttttct ttcaacttca tatactcttc acaagatagc ccgagcagtc cgagatagat
     4081 ctcttggttg tgttcgccaa gcaccggcgc tgtacacggg tcgcttggga cggttccgga
     4141 aaacttcatc ggtatacccg gtactttaaa ctccccacct tgagtgtgga agacgttgag
     4201 gatcatctct ctggcggcga tctgggcgtc gttgaccaca tctgccatcg tgtaaatcgg
     4261 gccgctcgga atgctttcct gctcgaaaag cgccagccac tcgtcccggg ttttttcgag
     4321 aagagcggcc tccaagatgg gcttgagtac ggcccaatgc tcgactctgc gctcattagt
     4381 ggcgaaacgc tcgtcgttgg ccagaaagct taacccggtc aaattgcaga acctcttcca
     4441 caggtcttga ttccctaccg caatattgat ccaatagtct tttgttttcg ctgtaaaaaa
     4501 tggggcgatc gagggatgca cgttgcccac gggaacgggg acgatcccgg tgcaggtgta
     4561 tcgggctaag gcgttttcga ggatcgacac catacagtcc aacatcgcga catcgatctt
     4621 ttgccctttt cccgttttct ccctcgcgtt cagcgcagcc aacaagctga tgcaaagata
     4681 gagcccggtg acgatgtcgg cgatcgaact ccccaccttt gtgggatgat ccgcgtctgg
     4741 gccggtgatg ctcatcattc cgctcatccc ctggatgatg atatcgtagg aggctttgtt
     4801 ccgataagga ccggtctgcc cgtaaccgca actgctgccg tagatgatcc gagggttgac
     4861 cttttccagt tcttcataag ataaccccag ccggtcggct acccccggtt tgaagttctc
     4921 gatgaagata tccgactttt ccaccaggcg caacaagacc tccttggcat ccgggtcctt
     4981 gaggttcatc gttacacttt tctttccgcg gtttacgctg acgaagtacg cgctcgtctg
     5041 attctgaaag ggcgggtagg ctcgagagtc atcgcctgtg atcggtcttt ctattttgat
     5101 cacttccgcg cccagatcgg ccagcaccat cgcgcaaaaa gggcctgcca gcactcgagt
     5161 caagtcgaca acacgtattc ctgttaaagg gccgctcata attgttcccc ctttcatttt
     5221 tcgagtcttt tattcgcttg gaatccgttt tgcggggtcg aaggccgcgc gtccgttttc
     5281 taaaatgcca tcgtacgatc gtatgttacg gtcttcaatg tgatcgaacc actgcgggat
     5341 cgagctcccc gggtagatgg tcttgatagg gtctggctcg agcgccgccc agatatactc
     5401 gttaccgttt cgagcttggg cgagcttctg cccgctggga tggatgatca tcgagctgcc
     5461 gaagaaatgg gaaccgctcg cgtcttttcc aacagcgttc gtcgcgaccc aatacacgtg
     5521 gttgtcgtac gcccttgcgc gattggtcag ctcccattgg tcg
//
LOCUS       DAOPXN010000150         5489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14836_length_5489_cov_5.969084, whole genome shotgun sequence.
ACCESSION   DAOPXN010000150 DAOPXN010000000
VERSION     DAOPXN010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14836_length_5489_cov_5.969084"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PKM49_08045"
     CDS             complement(<1..913)
                     /locus_tag="PKM49_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNY82786.1"
                     /translation="MGYLSMNIGIIGLPFVGKTTLFQLFSGVSGQTAAGSETRVVKVK
                     DPRVDTLSRIYQPKKTTYATIQFTDTPGLDPKAGAKERNRIFGILQGVDALLWVIGNF
                     EGSEKTPREQAEDIKSEIIIRDLEIVENGIDRLKHAKYKLEKPEEELLDFLERLHHPL
                     DQTLLPGAEYREEAEKRKAVGGLNLCSIKPSILLLNSDEASFAIETPEIKRIREDCAQ
                     NRLGWLELSGKFEMELNDFTPEERADLLTSIGLNETGIERLVREVYEHIGLISFFTVG
                     DEEVRAWTVREKTGAKDAAGKIHTDFER"
     gene            complement(930..1700)
                     /locus_tag="PKM49_08050"
     CDS             complement(930..1700)
                     /locus_tag="PKM49_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HNY82787.1"
                     /translation="MSEFKGNVFIDPKQLTSYGDAANDGAVQLSFTLPIPYGALAKEA
                     AKVFVRNLGFDTLDIVSMEDMQEGYTFFVVYAHTQKTIDVSKIKVMDVSFRVMSREET
                     DDFIQKKIGRPLVVVGATIESDAHTVGLDAILNMKGYHGDYGLERYKMFTVHNMGSQV
                     LCEEVVRKVKETKADALLISQIVTQKNLHIQNLTKLMDLLEIEGVRKGLLTIVGGPRI
                     SYELAVELGYDVGFGSGTVPSQVASYIATTLSERAELT"
     gene            complement(1697..3271)
                     /locus_tag="PKM49_08055"
     CDS             complement(1697..3271)
                     /locus_tag="PKM49_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HNY82788.1"
                     /translation="MARLSITLSRDDVSEAKKTAKAIVDDVFHFITKHTTDTIERTVL
                     RYFGVDGVDAGEAPLPNVVVDHLKKKGVLDTGSAYWISNACVATGKDPQTVAEAVGAN
                     ALDLTAIPSQPLETVYRFAQEKAVEALDRVESVRKTRDELLVQLRDNPKPYKYVIVAT
                     GNIYEDVSQAKAASEQGADVIAVIRSTAQSLLDYVPYGPTTEGFGGTYATQENFRIMR
                     QALDEIAAEQKRYIRLTNYASGLCMPEIAMMASWEGLDFLLNDSMYGILFRDINMKRT
                     FVDQYFSRLIVALSGITINTGEDNYLTTSDAIEKAYAVTASQLINEAFAKKAGMKEAY
                     MGLGHAFEIDPAIENGFLYELAHALLARELFPNATLKYMPPTKFMTGNVFKGYAMNTL
                     FNLASVFTGQDIQLLGMLTEAIHTPYLQDRYLAILNANYIFNNARLLGYELEVKKGGF
                     IERRAQEVLSQALTLLKHTQKVGLMNAIQEAQFADISRSQDGGKGLSGVHRKSEGYYN
                     PVLSELERRLGIEVAL"
     gene            complement(3341..4327)
                     /gene="ruvB"
                     /locus_tag="PKM49_08060"
     CDS             complement(3341..4327)
                     /gene="ruvB"
                     /locus_tag="PKM49_08060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNY82789.1"
                     /translation="MVVLRPKALEEYIGQNKIKESLQISILACKMRGDVLDHVLLVGP
                     PGLGKTTLANVIAHEMEANIHITSGPVIEKQGDLAAILTNLDRGDVLFIDEIHRLSRV
                     IEEVLYSAMEDFSLDILIGKGPSARSIRIDLNRFTLIGATTRSGLLTSPLRNRFGMIL
                     ELDFYSVEDLMRILRRSASLLGIGITQEAAQITAERSRGTPRIVNRLLRRVRDFATVK
                     RKSLIEAEDAVEALDSLEIDEYGLDYMDRKILKVLIEHYRGGPAGLRAIAAAVGIEAD
                     SISEVYEPFLIQTGFLIRTPRGREVTAKACQHLGYPLPKNTNQSALFEDTVT"
     gene            complement(4468..5334)
                     /gene="prmC"
                     /locus_tag="PKM49_08065"
     CDS             complement(4468..5334)
                     /gene="prmC"
                     /locus_tag="PKM49_08065"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNY82790.1"
                     /translation="MSAEKGTKPWTVQELVGWTAGFFGRKGLAKARLSAELLLAEALK
                     LDRTLLYSRWNDPVDAIPLEVFRSYIQRYVEGEPIEYILGYRWFMTYRLEVNPDVFIP
                     RVETEELIEWIVQKENRRPRWFMDLGTGSGAIGIALARAHPEATIVASDKSERALDVA
                     RRNARTHRVLDRILFYQADFLDGLDELVDPVEVVVCNPPYVGENERQEVSPATLRYEP
                     EMALFGGPDGLDFYRKTLEHRSLLRGKRVYFEFGLEQKERLEELFSPVGAVTCKRDMY
                     GHWRFLSVDFHS"
BASE COUNT         1175 a         1598 c         1342 g         1374 t
ORIGIN      
        1 cgcgctcgaa gtcggtatga atcttaccgg cggcgtcttt cgcgccagtc ttctcgcgaa
       61 ccgtccaagc gcgcacctct tcatctccta ccgtaaaaaa cgatatcagt ccgatatgtt
      121 cgtaaacctc gcgcaccaaa cgctcgatac cggtttcatt gagtccgatc gacgtgagaa
      181 gatcggccct ttcctccggc gtgaaatcgt tcaactccat ctcgaacttg ccgctgagtt
      241 ctaaccaacc gagccggttt tgagcgcaat cttcccgtat ccgtttgatt tcgggagttt
      301 cgatagcaaa gctggcctcg tcgctgttca ataggaggat cgacggcttt atcgaacaaa
      361 gattcaaccc cccgacggct ttacgttttt ccgcttcctc tcgatattcg gcgcccggaa
      421 gcagcgtttg atccaaggga tgatggagtc tttcgagaaa gtccaggagc tcttcttccg
      481 gtttttccag tttgtatttc gcgtgtttca atcgatcgat gccgttttct acgatctcca
      541 agtctcggat gatgatctcg gacttgatgt cctccgcctg ttctctcggc gtcttttccg
      601 acccttcgaa gttcccgatc acccataaga gggcgtcgac tccctgcaag attccgaaga
      661 tccggtttct ctctttcgcg ccggctttcg ggtctaaccc tggggtgtcc gtgaattgga
      721 tcgtcgcgta agttgttttt tttggttgat atatccgaga caacgtatcc acacgtgggt
      781 cttttacttt cactacccgt gtctccgacc ccgcagccgt ctggcccgag acgccggaga
      841 agagttgaaa gagcgtcgtc ttccccacaa aaggcaaacc gataatgccg atattcatcg
      901 aaagatagcc cacaagttct ccctcccgat taggtcaact ccgcgcgctc gctcaaggtt
      961 gtggcgatgt aactggcgac ttggctgggc acggtgcccg aaccgaagcc cacatcgtat
     1021 ccgagttcta cggccagttc gtaactgatc cgcgggcccc cgacgatcgt gagaagacct
     1081 ttcctcaccc cttcgatctc caataagtcc attaacttgg tgaggttttg tatgtgtagg
     1141 ttcttttgag tcacaatctg tgagattaag agcgcatcgg ccttcgtttc tttcaccttc
     1201 cggacgacct cttcacacaa gacctgactc cccatattgt gaacggtgaa catcttgtac
     1261 cgctctagtc cgtaatcccc gtgatagcct ttcatattca aaatcgcgtc cagcccgacc
     1321 gtatgggcgt cgctttcgat cgttgcgccc acaaccacga gaggccttcc gatttttttc
     1381 tgtataaaat cgtctgtctc ctctcggctc ataacccgaa aactaacgtc catcactttg
     1441 atcttcgaca catcgatcgt cttttgggtg tgcgcgtaaa cgacgaaaaa cgtgtagccc
     1501 tcttgcatat cttccatcga gacgatgtcc aaggtatcga acccgaggtt tcggacgaac
     1561 actttcgccg cttccttcgc caacgcgcca tacggaatcg gaagggtgaa agagagctgg
     1621 acggcgccgt cgttggcggc gtcgccgtag gatgtcagct gtttggggtc gatgaacacg
     1681 ttgcctttaa actcgctcat aacgccacct cgattcctaa acgtctctcc aattcactga
     1741 gaaccgggtt gtagtatccc tccgatttgc ggtgcactcc ggaaagtccc ttcccgccgt
     1801 cctgtgacct ggatatatcg gcgaattggg cctcctggat cgcgttcatc aaccctacct
     1861 tctgagtgtg ttttagaagg gtcagcgctt gacttaagac ctcttgcgcc cgcctttcga
     1921 tgaatcctcc ttttttgacc tccagctcat atcccagcaa ccgcgcgttg ttgaaaatat
     1981 agttggcgtt caagatcgcc agataccggt cttggagata gggcgtgtgg atggcctccg
     2041 tgagcattcc gagcagctgg atgtcttggc cggtgaagac cgaggccaga ttgaagaggg
     2101 tgttcatcgc gtagccctta aagacgtttc cggtcataaa ctttgtcggg ggcatatact
     2161 tgagtgtggc gttcggaaaa agctctcggg cgaggagcgc gtgggcgagt tcatacaaaa
     2221 acccgttttc gatggcaggg tcgatctcga atgcatgccc aagccccata tacgcctctt
     2281 tcataccagc ctttttcgcg aaggcctcgt tgatcagttg agacgccgtc accgcgtaag
     2341 ccttttcgat ggcgtcggag gtcgtgaggt agttatcttc tccggtgttg atcgttatgc
     2401 cggacaaggc cacgatcagc cgagaaaagt actggtccac gaaggtgcgc ttcatattga
     2461 tatcccggaa taggatgccg tacatcgaat cgttcaacag aaagtcgagg ccttcccaag
     2521 aggccatcat cgcgatttcg ggcatacaga gccccgaagc atagttagtc agccgaatat
     2581 agcgtttctg ctcggcggcg atttcatcga gcgcttgacg catgatccga aagttttctt
     2641 gcgtcgcgta cgtgccgcca aagccttcgg tcgtaggacc atacggaacg tagtccaata
     2701 ggctttgggc ggtggatcga atgactgcga tgacatccgc tccctgttcc gaagcggctt
     2761 ttgcctgact gacgtcttcg tagatgttac cggtggccac gatgacgtat ttgtagggct
     2821 ttgggttgtc gcgaagctgg actaaaagct catcccgggt ctttcgcacg gattcgactc
     2881 ggtctaaagc ctcaaccgcc ttttcctggg caaaccggta gacagtctcc agcggttgag
     2941 aaggaatggc cgtcaagtcc aacgcgttcg ccccgaccgc ttcggcgacg gtctgcggat
     3001 cctttcccgt tgccacacag gcgtttgaga tccaatacgc ggaaccggtg tccagcaccc
     3061 ctttcttttt caggtgatcc accaccacat tgggcaacgg cgcctcaccg gcgtccaccc
     3121 cgtcgacgcc gaaataccga agaacggttc gttcgatcgt atcggtcgta tgtttggtga
     3181 tgaagtgaaa aacgtcgtca acgatggctt tcgctgtctt cttcgcctct gagacatcat
     3241 ccctactcag cgtgatcgat agtctcgcca tactcctcct ccacaagtaa ttttatatcg
     3301 ctcaatatcg catatctttc ttcaatagcc ctccagaaag tcatgtcaca gtgtcttcaa
     3361 acagggcact ttgattcgtg ttcttgggca gcggataccc caaatgctga cacgccttcg
     3421 cggtgacctc tctaccgcga ggggtccgta tcaaaaatcc cgtttggatg agaaatggtt
     3481 cgtagacctc gctgatcgaa tccgcctcga tcccgaccgc cgcagcgatc gcgcgtaagc
     3541 ccgccggacc gccccgataa tgctcgatta aaaccttcag aatcttccgg tccatataat
     3601 cgaggccgta ttcgtcgatt tcaagagagt cgagcgcctc cacggcatcc tccgcttcaa
     3661 tgagggattt gcgcttgacg gtcgcgaagt cccgaactcg gcgcaggagg cggttgacga
     3721 tacgcggggt gccgcgggat cgttcggccg tgatctgggc cgcttcttga gtgatcccga
     3781 tccccagcag gctggcgctc ctcctaagga tccgcatcaa gtcttcgacc gaatagaaat
     3841 ccaactccaa aatcatcccg aatcggtttc taagaggtga ggtcagaagg ccgctgcgcg
     3901 tggtcgcgcc aatcaacgtg aagcgattga ggtcgatccg gatcgatcgg gcgcttggtc
     3961 cttttcctat caggatatcc aaagaaaaat cctccatcgc cgaatacaag acttcttcga
     4021 tcacgcggct caaacgatgg atctcatcga tgaacagcac gtctcctcta tccaggttcg
     4081 tcagaatcgc cgcaaggtcc ccttgctttt cgatgacggg gccgctcgta atatggatat
     4141 tcgcttccat ttcgtgggcg atgacgtttg ccagcgtcgt ttttccgagc cccggaggcc
     4201 ccactaaaag aacgtggtct aaaacatccc ctctcatctt acaggccagg atggagatct
     4261 gcaaactctc ctttatctta ttttggccga tgtactcttc tagcgccttc ggccttaaca
     4321 ccaccatctg ttggtcctct acccgcgaca cggagtcgac caaacgctcc gtttcaggcc
     4381 ctacccccca ttcctcattc ttcaggatga tcacctcggt tccagttctc acacgcctaa
     4441 tagccccgcg cgggccttat ataggcctta cgaatggaaa tcgacggata aaaaccgcca
     4501 atggccgtac atgtctcgct tacacgtcac cgcgccgacg ggcgaaaaaa gttcttcaag
     4561 tcgctctttt tgctctaagc cgaattcaaa atacactcgt tttcctcgta atagagatcg
     4621 gtgctccaac gttttccgat agaaatcgag cccgtcaggc ccgccaaaca aagccatttc
     4681 cggctcgtag cgcaacgtgg cggggctcac ttcttggcgt tcgttctctc ccacataagg
     4741 cggattgcac accactactt caaccgggtc gaccagctcg tccaacccgt ccaaaaaatc
     4801 tgcctgataa aagaggatcc gatccaggac ccgatgggtt ctcgcgttcc tacgcgccac
     4861 gtccaaagcg cgttccgact tgtccgaagc cacgatcgtc gcttccggat gcgcgcgcgc
     4921 caaagcgatc ccgatcgcgc cgctacctgt gccgagatcc ataaaccatc gaggtcttcg
     4981 gttttctttc tgaacgatcc actcgatcag ctcttccgtt tctacccgcg gaatgaagac
     5041 gtccggattc acttccaacc gataggtcat gaaccatctg taacccaaga tgtactcgat
     5101 gggttcgcct tcgacgtatc tttggatata ggagcgaaag acctccaagg gaatagcgtc
     5161 gacggggtcg ttccaacgcg agtataagag cgtgcggtct agcttcaacg cttcggctag
     5221 caatagctcc gcagaaagtc gggctttcgc cagccccttc cggccaaaaa aaccagccgt
     5281 ccagcccacc aactcctgaa cggtccacgg ttttgttcct ttttctgcgc tcacgtgcca
     5341 accctttccc tttatttcgc ttccttaacg tttgattgta ccataaaagg ctcaagatag
     5401 tgggaagggt gtgtgtcaag ttatggggtg caaacaagaa acaaggacag aatcgatcgc
     5461 acgattttta gcctatgtag tcatcgcgg
//
LOCUS       DAOPXN010000151         5482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14870_length_5482_cov_4.633499, whole genome shotgun sequence.
ACCESSION   DAOPXN010000151 DAOPXN010000000
VERSION     DAOPXN010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5482
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14870_length_5482_cov_4.633499"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            350..1672
                     /gene="cmr1"
                     /locus_tag="PKM49_08070"
     CDS             350..1672
                     /gene="cmr1"
                     /locus_tag="PKM49_08070"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HNY82791.1"
                     /translation="MQTIELTLKFNTPVFARGGSDGFEITPQSIKGLMRFWYRALLPN
                     VIDIHRCGQKTKQYCGMKYAEELLFGSTKQRSLFDVEVELKGMIQDFGSWGTTPRGRP
                     VFNPKEFLVTSGANYAAYGMSPTKPGEPIRKYLPAGAEVILSFVFRRKSLSNEMRDAF
                     LHLWGVISMFGGIGAKSRKGYGSFDIATTKVEGTMFDYSPTQTLFPESLRNVLDTVRS
                     TFAKMLAKLDTENALAFDLQPLMPVPPFPALTQVCWLHKKKYQNSDWRSVLDAIFGGS
                     GMMGQRSYRGNFQAYKTVKIKGMRLKHGEQNDGVQAVREIIYGQAPTFPHDQWEVSPT
                     ILGLPLLYQNMGNTPDSTGRNTGEVNNPTGRKASPLFVSIHKKNNDTFAFFLLIPSQI
                     SSNRTNDNKPILTVKPNIGNPEKEVLGNEDFEDLKKKLLKALEGGGVR"
     gene            1669..4209
                     /gene="cas10"
                     /locus_tag="PKM49_08075"
     CDS             1669..4209
                     /gene="cas10"
                     /locus_tag="PKM49_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HNY82792.1"
                     /translation="MKNNGFYWKRKLGAFFHDPVFKAFDVKTHEALAGKFLKVLGDEG
                     VEIQKSGDAPASAMDRLLLPYETSTNKQNRIIVDAADLSEFLHPLSGTPLEVKKTVYD
                     RYRNDARFLSEELVKWVESIVDKYPDDPCKLFHHLWWELPCKVPMIDFLPADTRAPYH
                     SIIDHLDMTAALEGCKIGAQVKPALLQVAIGPIQKFIAAARKTRDLWMGSYILSYLTF
                     QGIRLIGETFGFDHIIFPNMRHQTLLKEWLRKKEINVDDDTQDLPRDIASLPNRFLAV
                     VPSDQAEALAQQVKNAIEKVWREIAEKTAGKLKLSDTERQYWTMQTDLFPEFYYAIQN
                     WENPANFQVVFKKFFSDTDELDGFASELRKLSQLESYPVNSGSFYPFFYELTRRKLEA
                     VKATTAFGGYVDDRPVDGDQLSGEVKAILEDYRRRDGAASPGDKSERLGAINLIKREI
                     IEIQKDIPNKRTPSTTEIAIRNLEEEKRTRWLNLLREENQERENDTTFEKLPTPYYAI
                     LVMDGDKMGEWMSGKRAPELKDRLHEKAKNALTALDNNRKISLSKLKKAAITPSYHRA
                     ISRTLDHFSRFVKPVVEDKYNGLLIYAGGDDVLAFLPARTVFNCANDLRKIYSGIGKV
                     ELTVDVDGKNKEEYLFDQELCYKKENEKWFPMFPMMGSKATMSAGIALLNHKFPLSDA
                     LDIARDAEKCAKRGWIDLPPALSENEKADSRNAFSITVVRHSGQQTKVVSKWERNQTD
                     LLAKAQEIWTLLKQTKVSKKILYTFLEDIDSGEFTTEEWVERYLPYLIERRGNLTKRA
                     KDVVVEKFQEVLSGLVTKNDEIRTRKTFEDFFLLLSMGEFAQREVENR"
     gene            4206..5276
                     /gene="cmr3"
                     /locus_tag="PKM49_08080"
     CDS             4206..5276
                     /gene="cmr3"
                     /locus_tag="PKM49_08080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr3"
                     /protein_id="HNY82793.1"
                     /translation="MKNFDILRFNPESALLFRDTRGFGFSDNARTVFPTSEPFYGAIR
                     TAFLRKNKTNLQNQKAVEKYREQIGDRDHPGKFRFIGPFPFLKEDGRTRYFLPTPSHL
                     TKWDDGKIGFLQPNPELSFSYRGISLHTLWDGKTEQGSRAAEAYPWIDWEGMSKIKEG
                     KPPAKESLVKETVFYEIEHRTGIGLRSDRKNAREEMIFRVRSFRFKEEAGLYLCVLNG
                     AELLEGIDTVLLGGKSGVATTELEKNVPGSLFQKVEDSRKTVIFVTPAIFRNGFLPTG
                     SENSFFGATVKAVCNGKPYAIGGWDLIARRPKPLMHAVPIGSTYFIEGSLNTTTITEL
                     RGEFGYGSYIECKRYKEDNYGK"
     gene            5329..5482
                     /locus_tag="PKM49_08085"
                     /pseudo
     CDS             5329..5482
                     /locus_tag="PKM49_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924294.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
BASE COUNT         1533 a         1377 c         1403 g         1169 t
ORIGIN      
        1 ggctttagtc cggcatgcct tttctttttc caagagaaac ctgtcccatg ccagtttttc
       61 ctccgtgttc tctgtgccct ctgtgcgaac cctgtttttt gatcttggga acgaatgaaa
      121 ggggtatcgc gccgtcgcga agagaagggg cgcttacgcc gcgattaccg caattgggaa
      181 ggcacggaac caccggactt catttcggca tatcaacagg tttttcactc ggaacgctct
      241 ctttttcacc cagcgcgctc cgtgtagagc aaaagacgac tctatttcat tatgcgcaag
      301 cgcttaatga gagaaaaaga aaaattcaga aagaaaggag ggtatttttt tgcaaacgat
      361 cgagttaacg ctgaagttta acacaccggt atttgcgagg ggaggctccg acggatttga
      421 aattacccct caaagcatca aagggctcat gcgtttctgg tatcgcgcgc tgcttccgaa
      481 cgtgatcgac attcaccgtt gcggacagaa gaccaagcag tattgcggaa tgaagtacgc
      541 cgaggagctc ctgtttggca gcacaaaaca gcgcagcctg ttcgacgtgg aagtggaatt
      601 aaaaggaatg atacaggact ttgggagttg ggggacaact ccaagaggaa ggcctgtgtt
      661 taacccaaaa gaatttctcg tgacgagcgg cgcaaactac gccgcgtacg ggatgagccc
      721 tactaaaccg ggagaaccca tacgtaagta cctgccggcc ggagcggaag tgattctgtc
      781 tttcgttttc cgccggaaga gcttgtcgaa tgagatgcgc gacgccttcc tccacctatg
      841 gggagtcatc agcatgtttg gcggaatcgg cgcaaaatcc cgaaaagggt acggaagctt
      901 cgacatcgcc acgaccaaag tagaaggaac gatgttcgac tattccccta cccaaactct
      961 gtttccagaa agtctacgaa atgttttaga taccgtgaga agcactttcg ccaaaatgct
     1021 ggcaaagttg gacacggaaa atgccctcgc gtttgacttg cagcctttga tgcctgtccc
     1081 accatttcca gccctcactc aggtatgctg gctccacaaa aaaaagtacc aaaactccga
     1141 ctggagatca gttttagacg cgatctttgg aggatcgggg atgatgggcc aacgaagcta
     1201 tcgagggaat tttcaggcct ataagaccgt aaaaataaag ggaatgaggt tgaaacacgg
     1261 cgaacaaaat gatggggtac aagcggtgcg ggaaatcatc tatggtcagg cgccaacctt
     1321 cccccacgat caatgggagg tttcaccaac gatactcggc cttcctttac tgtatcaaaa
     1381 tatgggcaat actccggata gcaccggccg taacaccggt gaagtgaata acccaacagg
     1441 gcggaaagct tctcccttgt tcgtttcgat ccataaaaag aacaacgata ccttcgcctt
     1501 tttcctgctc attccatctc agatttctag caatcgaacc aacgacaaca aacccatttt
     1561 aaccgttaag cccaacattg gaaatccaga aaaagaggtc ttaggcaatg aagatttcga
     1621 agatctcaag aaaaaactct tgaaagcgct cgagggtggt ggtgtgaggt gaaaaacaat
     1681 ggcttctatt ggaaacgaaa gctcggagcc ttcttccacg acccggtctt taaagccttc
     1741 gacgtaaaaa cccatgaagc ccttgcgggt aaattcctca aggttttggg cgatgaaggc
     1801 gtcgagatac aaaaatcagg agacgccccc gccagcgcga tggaccggct gttgctgcct
     1861 tatgaaacat cgactaacaa acagaaccgg attattgtcg acgctgccga tctgagcgaa
     1921 ttcctgcacc ctctctccgg tactcctttg gaggttaaaa agaccgtcta tgaccgctat
     1981 agaaacgatg cccggttcct ctccgaagag ctcgtaaaat gggtcgaatc gatcgtcgac
     2041 aaatacccgg acgatccctg caaattgttc catcacctct ggtgggaatt gccctgcaaa
     2101 gtgccgatga tcgacttcct acccgccgac acgcgcgcgc cctatcactc catcatcgac
     2161 cacctcgata tgaccgccgc cctcgagggc tgcaaaatcg gcgctcaagt caaacccgcg
     2221 ctcctacagg tggcgatcgg gccgatccaa aagttcatcg ccgctgcgcg caaaacccgt
     2281 gatttatgga tgggcagcta tatactttct tacctgacct tccagggcat acgccttatc
     2341 ggagaaacct tcgggttcga ccatatcatc ttcccgaata tgagacacca gacactcctg
     2401 aaagagtggc ttcgaaagaa agagattaat gtcgacgatg acactcagga cctaccgcgc
     2461 gacatcgcct ccttacccaa ccgatttctg gcggtcgttc catcagacca ggctgaagcg
     2521 ctcgcgcaac aggtgaaaaa cgcgatagag aaagtttggc gcgaaatcgc agagaagaca
     2581 gcgggaaagt tgaagctcag cgatacggaa agacagtatt ggactatgca gacggactta
     2641 ttcccggagt tttattacgc gatccagaac tgggaaaacc cggcaaattt ccaagttgtc
     2701 tttaagaaat ttttctctga tacagacgaa ctcgacggtt tcgcgagcga attacggaaa
     2761 ctctctcagc tcgagagtta tccggttaac agcggcagct tctacccgtt cttttatgaa
     2821 ctgacgcgtc gaaaactgga agcagtgaaa gcgacgaccg cttttggggg ctacgtcgac
     2881 gaccgccccg tggacggaga ccagctgagc ggggaagtga aagccatcct cgaggactac
     2941 cgacggcgtg atggcgcggc gtcgccgggg gacaaatcgg agcgattggg cgccatcaac
     3001 ctcatcaaac gagagatcat cgagattcag aaagacatcc caaacaagcg cacgccatcg
     3061 acaaccgaaa tcgcaatacg gaatctggaa gaagagaaac gaacgcgatg gttgaactta
     3121 ttgagagaag agaatcagga acgcgagaat gatacaacgt tcgaaaagtt gccgacccca
     3181 tattacgcga tactcgtgat ggacggagac aagatgggcg agtggatgtc cggaaaacgg
     3241 gcgccagagc tgaaagaccg tttgcacgag aaagcgaaaa atgcgctaac cgctctcgat
     3301 aacaatagaa aaataagtct ttccaaatta aaaaaggcgg cgatcactcc cagttaccac
     3361 cgcgcgatct cgcgaacgct ggatcatttc tcccgatttg tcaagccggt agttgaagac
     3421 aaatacaacg gattgctcat ctacgccgga ggcgacgacg ttctcgcttt cctgcccgct
     3481 cgaaccgtct ttaattgcgc caatgacctg cgcaaaattt actctggtat cggaaaagtc
     3541 gaactgacgg tggatgtcga cggtaaaaat aaagaagaat acttgttcga tcaggagctt
     3601 tgttataaaa aagaaaatga aaaatggttc ccgatgttcc cgatgatggg aagcaaagcc
     3661 acgatgagcg ccggtatcgc cctgctcaac cacaaatttc cgttatcgga cgcgctcgat
     3721 atcgcgcgag atgctgaaaa atgcgcgaaa agaggctgga tcgaccttcc ccctgcgctt
     3781 tctgaaaacg aaaaagccga ttcgagaaac gctttctcga ttacggtggt tcgacactcc
     3841 ggccagcaaa ccaaagtggt atccaaatgg gaacggaatc aaacggacct cctggctaag
     3901 gcgcaagaga tctggacgct gctgaaacaa accaaagtga gtaaaaaaat cctctacacg
     3961 tttttggaag acatcgattc cggcgagttc acgacggaag aatgggtaga gcgatacctt
     4021 ccctatctca tcgaacgtcg cggaaacctc acaaagcgag ccaaagacgt cgttgtagag
     4081 aaatttcagg aagtgttaag cggattagtc acgaaaaacg atgaaatacg aacgcgtaaa
     4141 acctttgaag atttcttctt gctgctctcg atgggagagt tcgcgcagag agaggtggaa
     4201 aaccgatgaa aaacttcgac atcctccgtt tcaaccctga aagcgccttg cttttccgcg
     4261 acacacgcgg atttggtttt tcggataacg cccggactgt gttcccgacc tcagaaccgt
     4321 tctatggcgc gattcgtacg gcctttttga ggaaaaataa aacaaacctt caaaaccaga
     4381 aggccgttga aaaataccgt gaacagatcg gcgatagaga ccacccgggg aaattccgct
     4441 tcatcggccc tttcccattt ctgaaagaag atgggcggac ccggtacttc ctccccaccc
     4501 cttcccatct gacgaaatgg gatgacggaa agatcggttt tttacagcca aacccggaac
     4561 tatccttttc ttatagaggc atctccctcc atacgctttg ggatggaaaa acggagcaag
     4621 ggtctcgagc ggcggaagct tacccgtgga tcgactggga aggaatgtcc aagataaagg
     4681 agggaaaacc gcccgccaaa gagtcgctgg tgaaagaaac tgtcttctac gaaatcgaac
     4741 accgtaccgg aatcggtctc cgatccgatc gcaaaaacgc gcgggaagag atgatcttcc
     4801 gggtccgctc tttccgtttt aaagaggaag ccggcctata tctatgcgtt ttaaacggcg
     4861 ccgagcttct ggaaggaatc gatacggtat tgctgggagg caaatcgggt gtggcgacaa
     4921 ccgaactcga aaagaacgtg ccgggatccc ttttccagaa agttgaggat tcacgaaaaa
     4981 ccgtgatttt tgtgactcct gcgatcttcc ggaatggctt tcttccgacg ggttccgaga
     5041 acagtttttt cggggcgacc gtgaaagcgg tgtgcaatgg aaaaccgtat gcgatcggcg
     5101 gatgggacct catcgcccgt agaccgaaac ctttgatgca tgcggtgcca ataggttcta
     5161 cctacttcat agagggttcc ctcaatacga cgacgatcac ggaattacgg ggcgaattcg
     5221 gatatggctc atacatagag tgcaaacggt ataaggagga taactatgga aaataagatt
     5281 ttggcgctat atgcggaaag tcagattcac gcggggaagg gcagccatgt cggcatcgtc
     5341 gaattcgatg caaaggtcgc gagcgccgat gcaatgctgg acgcggcgat cgaagccggt
     5401 gccgacgatg tgatctcgag cgacgatggc catcagatca tcacgacgcc ggacacgttg
     5461 aacgatgtcg ccaaggcgct cg
//
LOCUS       DAOPXN010000152         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_14938_length_5462_cov_5.391899, whole genome shotgun sequence.
ACCESSION   DAOPXN010000152 DAOPXN010000000
VERSION     DAOPXN010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14938_length_5462_cov_5.391899"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PKM49_08090"
     CDS             complement(<1..507)
                     /locus_tag="PKM49_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82794.1"
                     /translation="MKRVLIRTHGGERIGLGHLMRCLSIAKGLKRYAKTVRFEVEWIV
                     NREAIPLLEAEGFRAKAAERFSNQEEGLFEGEKPDAILFDAYGAGNPYLTFLKTRAKT
                     LILIDDNNDQYASADVDAVINGNIHARGLPYADTLPRAERWVGPELLAMKEEHWDTAD
                     SDAPVPGNV"
     gene            complement(511..1650)
                     /gene="pseC"
                     /locus_tag="PKM49_08095"
     CDS             complement(511..1650)
                     /gene="pseC"
                     /locus_tag="PKM49_08095"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HNY82795.1"
                     /translation="MMNIPYARQWIEEEDIERVAKVLRGDWLTQGPAVDAFEQAVASY
                     VNAKYCVAFNSGTAALHAAMYAAGIQPGDEVLTTPITFVATSNSALYLGGKPLFVDID
                     PKTYCLDITKIERAITPATKVIAPVDYAGYPIDIDPILKMAREKGLVVIEDAAHALGA
                     DRNGEKVGPQADMTMFSFHPVKHITTGEGGVIVTNDETYYERMRLFRTHGITKEPAAF
                     LHEAEGPWYYEMQALGFNYRITDFQCALGSSQLEKLPRSLARRRQIAEKYYAGLKDVE
                     GVLLPPIPENPHTTHAYHLFPVQFMRKDRKEVFLSLKEKGIFGQVHYIPAHLQPYYRE
                     SYGFKPGDYPRSEAFYQREISIPMFPKMTDEEVDYVISTILEICL"
     gene            complement(1739..2680)
                     /locus_tag="PKM49_08100"
     CDS             complement(1739..2680)
                     /locus_tag="PKM49_08100"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNY82796.1"
                     /translation="MKTLITGGAGFIGRWLTQRLLEDSERLTIVDDLSNGRVENIAEF
                     FGNPRLTFVEGDIRRHDVLAEVFKTPYDIVYHLAAEINVQNSIDDPRQTFERDVMGTF
                     NILETCRQTKTPLVFMSTCMVYERSQSETGIDESHPTKPASPYAAAKLSGEALALSYY
                     YAYQMPTTVIRPFNTYGPYQKTNGEGGVVAIFIKNQLRGLPLRIFGDGTQTRDLLYVR
                     DCVDFIAKAGTSSSALGQIINAGTGEDIAIKDLAKAIANGTGQIVHVPHIHPQAEIQK
                     LKCDARKAKRLLDWEPKYTLKQGLAETTEWIRENPDL"
     gene            complement(2610..3719)
                     /locus_tag="PKM49_08105"
     CDS             complement(2610..3719)
                     /locus_tag="PKM49_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNY82797.1"
                     /translation="MFENKNILIIGGTGSVGRALLDKLLTCNPNVIRVFSRDEFKQFE
                     IEHELQLFNLYENGHSIPAVDKVRFLIGDVRDKDRVSRAMEGIDVVFNVAAMKHVPAC
                     EYNPFEAVKTNVIGVQNVIECALKVGVQTVVYTSTDKAASPTNTMGATKLLAERIMSA
                     AQFTRGPAKTKFATVRFGNILMSRGSVLPLFIEQSRKGKPLTLTDPSMTRFVMSKERA
                     VELILQAARISLGGETFVLKMPVVKIGDLAQAVAEFYANDHIEVIGKRPGEKLYEELM
                     TEEESVRALEFDEMFGILPFFGEKAKYESFYKGLAASAAIRQYSTEDEPVLPYPEVKE
                     MIWRILRQEEAAYENSNNGRGRLHRPMADTAASRG"
     gene            complement(3873..4379)
                     /locus_tag="PKM49_08110"
     CDS             complement(3873..4379)
                     /locus_tag="PKM49_08110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY82798.1"
                     /translation="MIYNENIKLLPIKTDDTDFVLSLRNDLEIARRFFSDPPVYESVH
                     TKWLSQRDEKDLDFIIWYEGSRSGRISITHVNYRHSKAEYGIVIHPDYRGRNIAFRAS
                     QLLINYVFKELPIRKLSLQVFADNNIAIRLYEKLGFSREGLLKQEYLRSGVYQDVLLM
                     ALFKENWG"
     gene            complement(4383..5045)
                     /locus_tag="PKM49_08115"
     CDS             complement(4383..5045)
                     /locus_tag="PKM49_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNY82799.1"
                     /translation="MRRIMSIAAHPDDEMLGCGGTLALFTEKGNLVKSVILGEGMLSR
                     KDPQTTLSSLQNDARNANRALGIHNVVFYELPDNAFDTVSLLSIVQILEKEILLFKPD
                     IVFTHYGNDLNIDHRRTFEAVMTACRPQPGFINPDIYSFFIPSSTDWIDGVAFPPFSP
                     NFYVDISTTIERKLSALSKYETEMKEYPHSRSIEALRVFSKYWGAVSVGSMPISSSSY
                     GA"
     gene            complement(5042..>5462)
                     /locus_tag="PKM49_08120"
     CDS             complement(5042..>5462)
                     /locus_tag="PKM49_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004227554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNY82800.1"
                     /translation="QETKISAIRVVEEMDAGPIYLKKPLSLLGTAEEIFRRASHIIFQ
                     EMIPMIIQNPIIPKEQEGAPTYFKRRTQAQSSIEECQTLDKLYDHIRMLDGEGYPPAF
                     LETEEFIFEFTRASLKTGFIQADVKIKKKTEQEGERM"
BASE COUNT         1088 a         1451 c         1490 g         1433 t
ORIGIN      
        1 gacgtttccg ggtacgggcg cgtcgctatc tgccgtatcc caatgctctt ctttcatcgc
       61 taaaagctcg gggccaaccc aacgctccgc tcgggggagg gtgtcggcgt atggcagtcc
      121 ccgcgcgtgg atattaccgt tgatcacggc gtccacgtcc gcggaagcgt attggtcgtt
      181 gttgtcgtcg atgaggatca gggtcttggc gcgcgttttg aggaaagtga gatatgggtt
      241 tccagcgcca taggcgtcaa agaggatggc atcgggtttt tcaccttcga acaacccttc
      301 ctcttgattg gaaaacctct ccgcggcctt cgcgcggaat ccttcggctt cgaggagcgg
      361 tatcgcctcc ctgttgacga tccattccac ttcgaatcga acggttttcg cgtaacgctt
      421 tagccctttc gcgatggaaa ggcagcgcat cagatggcct aagccgatac gttcgccgcc
      481 gtgtgtccga atgaggacgc gtttcatcct tcagaggcag atctcaagga tagtggagat
      541 cacgtaatcc acttcctcgt cggtcatctt tgggaacatt gggatgctga tttccctttg
      601 gtagaaagct tcggagcggg gataatctcc gggtttgaag ccatagctct ctcggtagta
      661 cggctgcaga tgcgccggga tgtagtggac ctgtccgaag attcctttct ctttcaggga
      721 caaaaagacc tctttccggt ctttacgcat aaactgcacc gggaacaggt gataggcgtg
      781 ggtggtatga gggttctctg gtattggcgg taggaggaca ccctccacgt ctttcagccc
      841 cgcatagtac ttttccgcga tttgtcgccg tctggccagc gagcggggga gcttttcgag
      901 ttgggagctg ccgagcgcgc attggaaatc ggtgattcgg tagttgaatc ccaaggcctg
      961 catctcgtag taccaagggc cttccgcttc gtggaggaag gcggccggtt ctttcgtgat
     1021 cccgtgcgtg cggaagaggc gcatacgttc atagtaggtc tcgtcgtttg tgacgatcac
     1081 cccgccctct cccgtcgtga tgtgcttcac cggatggaag ctgaacatcg tcatatccgc
     1141 ttgggggccc accttttcgc cgtttcgatc cgcgcccaaa gcgtgggcag cgtcttcgat
     1201 caccacgaga cccttctccc tcgccatttt caggatcggg tcgatgtcta tgggatagcc
     1261 agcgtagtcg accggcgcga tgaccttcgt cgcgggcgtg atcgcccgtt cgattttcgt
     1321 gatgtccaag cagtaagttt ttgggtcgat atcgacgaat aacggtttcc cgcctaaata
     1381 gagggcggaa ttcgacgtgg cgacgaaggt gatgggggtc gtcaggacct cgtcgccggg
     1441 ttgtatcccc gccgcgtaca tcgccgcgtg cagggctgcg gttcctgaat tgaacgccac
     1501 acagtacttc gcgttcacgt aactcgcgac ggcttgctcg aaggcgtcca ccgcgggtcc
     1561 ttgcgtcagc cagtcccctc tgagaacttt cgccaccctc tcgatatcct cttcctctat
     1621 ccattgtctg gcgtatggaa tattcatcat tctcttctct ccttcgtttt gagcccgatt
     1681 cgggttcaga cagggttcgg ccctcttcca cgcggttcgc ccagggggac gcttgccctt
     1741 acaaatccgg gttttcgcgt atccattcgg tcgtctcggc caacccctgt ttcaacgtgt
     1801 atttcggttc ccaatcgagt agtcgcttcg ccttacgggc gtcgcatttc agcttttgta
     1861 tctccgcctg cgggtggatg tgcggcacgt gaacgatctg gccggttccg ttggcaatcg
     1921 ctttggcgag gtctttgatc gcgatgtcct cgcccgtgcc cgcgttgatg atctgcccga
     1981 gggcgctcga gctcgttccc gctttcgcga tgaagtcgac gcagtctctt acgtacaaaa
     2041 ggtcccgggt ttgggttccg tcgccaaaga tacgtaaagg caaccctcgt aactggtttt
     2101 ttatgaagat ggcgaccacg ccaccctctc cattcgtctt ctggtaaggt ccgtaggtgt
     2161 tgaaggggcg gatgacggtg gttggcattt gataggcgta atagtatgag agcgcgagcg
     2221 cctcgccgct gagcttcgcg gcggcgtacg gggaggcggg tttcgtgggg tgcgattcgt
     2281 caatcccggt ttcggactgg cttcgttcgt agaccatgca agttgacata aagaccagag
     2341 gggttttggt ttggcgacag gtttcgagta tattgaaagt acccatcaca tcgcgctcga
     2401 aggtttgtcg cgggtcgtcg atcgaattct ggacgttgat ctccgccgca aggtgataga
     2461 cgatgtcgta gggcgttttg aagacttccg cgagaacatc gtgtcgacgt atgtccccct
     2521 cgacgaaggt caaccgcggg ttaccgaaga actcggcgat gttctccaca cggccattgg
     2581 aaagatcgtc gacgatggtc agccgttcgc tatcctcgag aagccgctgt gtcagccatc
     2641 ggccgatgaa gcctgccccg cccgttatta gagttttcat acgccgcctc ctcttgacgt
     2701 aaaatgcgcc atatcatctc tttgacctcc gggtagggca ataccggctc gtcttcggtg
     2761 gaatactgcc ggatcgcggc ggaggcagcc aaccctttat agaaactctc gtatttcgct
     2821 ttttctccga agaagggcaa gattccgaac atctcgtcga actccaacgc gcgcacactt
     2881 tcctcttcgg tcatcaattc ctcgtacagt ttttctccgg ggcgctttcc gatcacttcg
     2941 atatggtcgt ttgcatagaa ctccgcgacg gcttgcgcga ggtcgccgat cttcacgacc
     3001 ggcatcttca ggacgaatgt ttcgccgcct aaagagatac gggccgcctg aaggatcaac
     3061 tcgacggcgc gttcttttga catcacgaat cgcgtcatcg atgggtcggt cagagtcagc
     3121 ggtttgcctt tcctgctctg ctcgatgaag aggggtagga cgctcccacg gctcatcagg
     3181 atgttcccaa aacggacggt ggcgaatttg gttttcgcgg ggccccgtgt gaactgcgcc
     3241 gcgctcataa tccgttcggc gagcagtttt gtggcgccca tggtgttggt tggagaagcg
     3301 gccttatctg tgctggtgta gacgaccgtt tgaaccccta ccttcagcgc gcactcgatc
     3361 acgttctgca cgccgatgac gttggtttta acggcttcga aggggttgta ttcgcaggct
     3421 ggcacgtgtt tcatcgcggc cacgttgaag acgacatcga ttccctccat cgcccgggaa
     3481 acgcggtcct tgtcgcgcac atccccgatg agaaagcgga ctttgtcgac cgcgggaata
     3541 gagtgcccgt tttcatacag gttgaaaagc tgcagctcgt gttcgatttc gaactgcttg
     3601 aattcatctc gggaaaagac gcgaatgaca ttgggattac aggtgagcaa tttgtcgagg
     3661 agcgctcgcc ccacgcttcc ggttccaccg atgatcaaga tatttttatt ttcaaacatc
     3721 ttcggcacct accttctgga ttttaccgca cgttcattta tcggaatgag agggagaata
     3781 cttaaggagc gtgcgggaag gcgcttccgg catcggcact tctctacgtg gttcctcgga
     3841 tttttctcgg ccttctgttt acagttctgc ccttatcccc agttttcctt aaatagcgcc
     3901 atgagtaaaa catcttgata cacgccgctc ctcaaatact cctgtttcag aaggccttcg
     3961 cgactgaacc cgagcttttc gtatagcctt atggcgatat tgttgtccgc aaacacttga
     4021 agggacagtt tcctgatggg cagttctttg aacacgtagt tgatcaagag ctggctcgcg
     4081 cgaaaggcga tattcctccc acgataatcc ggatgtatca cgatgccata ttcggctttc
     4141 gagtggcgat aattcacgtg cgttatggat atccttccgg atcggctccc ctcataccaa
     4201 atgatgaaat cgaggtcttt ctcatccctt tgggaaagcc acttcgtgtg cacgctctcg
     4261 taaacggggg ggtcggaaaa gaaccgcctg gcgatttcca aatcgtttct gagcgaaaga
     4321 acaaagtccg tatcgtcggt ttttataggc aagagtttga tattttcgtt atatatcatt
     4381 tttcacgctc cgtacgagga tgaagagatc ggcatactcc ctaccgacac ggcaccccag
     4441 tatttcgaaa agacgcgtag agcctctatc gatctagaat gagggtattc cttcatttcc
     4501 gtttcgtatt tacttaaggc tgacagtttc cgttcgattg tagtagagat atcgacgtaa
     4561 aaattcgggg aaaaaggcgg aaaggccacc ccgtctatcc aatcggtcga ggaaggtatg
     4621 aaaaaggaat agatatctgg gtttataaac cccggctgcg gtcggcaagc ggtcataacg
     4681 gcctcgaaag tgcggcggtg gtctatgttc aagtcatttc cgtaatgggt gaaaacgatg
     4741 tccggtttga agaggaggat ttctttttcg aggatttgta cgatagaaag cagggaaacc
     4801 gtgtcgaacg cgttatcggg caactcataa aacaccacgt tgtgaatacc gagtgcgcga
     4861 ttcgcgttcc gcgcatcgtt ctgaagagaa gagagcgtgg tctgcgggtc ttttcgagaa
     4921 agcattccct cgcctaaaat caccgacttc actagatttc ccttttccgt gaaaagcgcc
     4981 aaggttccgc cacaccccaa catttcgtcg tcaggatggg cggcgatgga cataatccgt
     5041 ctcacattct ttccccctct tgctcggttt ttttctttat cttgacatcc gcttggataa
     5101 atccggtttt taacgaagcc cgcgtaaatt caaagatgaa ttcctcggtc tctaggaagg
     5161 ccggcggata gccttctcca tcgagcatac gtatgtgatc gtaaagctta tcgagcgttt
     5221 ggcactcctc tatcgagctt tgggcttgtg tgcgccgctt gaagtatgtc ggtgcgcctt
     5281 cctgctcttt cgggataatg gggttctgta taatcatcgg gatcatttcc tggaaaatga
     5341 tatgcgaagc ccttctaaaa atctcctcag cggtgcccag cagtgagagg ggtttcttta
     5401 aatagatagg acccgcgtcc atctcttcca cgactcggat cgcgctaatt ttcgtttcct
     5461 gg
//
LOCUS       DAOPXN010000153         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_15189_length_5395_cov_5.135581, whole genome shotgun sequence.
ACCESSION   DAOPXN010000153 DAOPXN010000000
VERSION     DAOPXN010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15189_length_5395_cov_5.135581"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..623
                     /locus_tag="PKM49_08125"
     CDS             15..623
                     /locus_tag="PKM49_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY82801.1"
                     /translation="MRAKIVTESHQHFDHAAHKRVGGSFELLTKPEKRTFPKVSIEGF
                     PAFHDKEQGMKRGRNIMFKFSFPKAPTPQKEGEQGGAAPEEAPADAPFTILHAGDLGH
                     LLTAEEVQALGKIDVLCVPVGGTYTLDPQEAADFTSALNPNLVIPMHFKTQLMDNVAE
                     LIEFLRVCERPVVRRDSLNFSKINMEKAKGYCVVLTPLVFEK"
     gene            complement(676..2037)
                     /locus_tag="PKM49_08130"
     CDS             complement(676..2037)
                     /locus_tag="PKM49_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014729948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNY82802.1"
                     /translation="MKDLTNGSIIKHIFSLSLPTIIGFSLQMVYDLVDIFWIGMISSE
                     ALAGVTIFGSIFWLIGSLNDIVGQSSISVISQNFGRKDLPATQIAIEQTITFKVLLAL
                     VAGALMALFLNPLISFFTNEPAVAKAAFDYGYIRLFLLPIMFASYSVNTILRCLGDAK
                     TPMVLMLFSSVLNVILDPLFMFATVPFTSIPGFGLGVFGAALATVISQSTAFFIGFAY
                     LLSKKNLVRPSIKGLFRLHWETDKKLLTIGLPTGFETISRNVSQIIVMKFVSTFGTAV
                     IAATGIAGRILGIAFTPLMGVSMGASTIVGQNLGAEKVERALDTTRWAARIVTVIIGF
                     FVLLCWLYGDRVMGIFANDREIIQTGVLYMTYGMSGMVALAYAFGLSSGFGGAGYNYP
                     YVVSSTVSRWVVMIPFLFVTVSVLKLGFVWVTLAYVASDVSEALILWRYYTRGIWKTK
                     RVA"
     gene            complement(2138..2260)
                     /locus_tag="PKM49_08135"
     CDS             complement(2138..2260)
                     /locus_tag="PKM49_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82803.1"
                     /translation="MIKIQNQFIYRQDRRVPVESERVLKEKNTDYAGIPRARYR"
     gene            complement(2600..2812)
                     /locus_tag="PKM49_08140"
     CDS             complement(2600..2812)
                     /locus_tag="PKM49_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82804.1"
                     /translation="MDQQIDEMLKYLKATKLPYGYFHLGDYFFLLQDDTIKEILFDNN
                     VTRTIDPSFNLLTTLKAKKIWMDNNF"
     gene            complement(2785..3108)
                     /locus_tag="PKM49_08145"
     CDS             complement(2785..3108)
                     /locus_tag="PKM49_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82805.1"
                     /translation="MRIRTILIVCALLVALAFGAPEELSFNNLQIVGNIPEGAFASIV
                     VRNEEKFAKGFTSFDAVVMVQRIYLYHNGVTQLYDGIEIKAGAYSIRLSKEQLEHWIN
                     KLTRC"
     gene            complement(3343..3468)
                     /locus_tag="PKM49_08150"
     CDS             complement(3343..3468)
                     /locus_tag="PKM49_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82806.1"
                     /translation="MKDKRNVKTNRLERLLLRSELEKEMEKRMNEIWVLQWPRYI"
     gene            complement(3914..4468)
                     /locus_tag="PKM49_08155"
     CDS             complement(3914..4468)
                     /locus_tag="PKM49_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HNY82807.1"
                     /translation="MNGLISILPPFFREEVLYFWDLLSERFGVDRVKLTGTPHITWNY
                     AERYLLDDPQAFCRSLARSLRPLRCRTNGLGIFSGKRPVIYIAFMMDAELERFHRRIW
                     EAIAPVAVTPNKRYHPERWIPHVTLAMEDLTLENIGPITAFLCAYKWKYAFKIESLSW
                     VTRNRGENMTVEKTFGINTEEASH"
BASE COUNT         1349 a         1411 c         1357 g         1278 t
ORIGIN      
        1 cgaattttcc gaagattcgt gcaaaaatcg tcacagagag tcaccagcac ttcgatcacg
       61 ctgctcacaa aagggttgga ggctctttcg agctactcac gaaacccgaa aaaaggactt
      121 tccccaaagt tagtattgag ggttttccgg ccttccacga caaggagcag ggaatgaagc
      181 ggggcaggaa cattatgttc aagttctctt ttccgaaagc ccccacccct caaaaagagg
      241 gagaacaggg tggcgcagcg cctgaagaag ctccggcaga cgcaccgttc accattctac
      301 acgcggggga cctgggccat ctgttgacgg ctgaagaagt gcaagccctt ggaaaaatcg
      361 acgttctgtg cgtaccggtg ggcggaactt acacgctgga ccctcaagag gcggctgatt
      421 ttaccagcgc tttgaacccg aacctcgtca ttccgatgca tttcaagacg cagctgatgg
      481 ataacgtcgc cgagctcatt gagttcctga gggtttgtga gcggcctgtg gtgaggcgcg
      541 attccctgaa cttttccaaa atcaacatgg agaaagcgaa gggatattgc gtggtgctca
      601 ctcctttagt tttcgagaaa taaagcagaa agggacaacc cctcttttcg aggttgtccc
      661 gttatcggcc agcgatcagg ccacgcgctt cgttttccag ataccgcggg tgtagtatcg
      721 ccaaaggatc agggcctccg acacatccga ggcgacgtaa gccaaggtca cccacacgaa
      781 tccgagtttc agcaccgaga cggttacaaa caagaaggga atcatcacga cccaacggga
      841 aaccgtactg gacacgacgt acgggtaatt atagccggcg ccgccgaagc cgctggacaa
      901 cccgaaagcg taagcgagcg ccaccattcc ggacatcccg taagtcatat acaacacgcc
      961 cgtttgtatg atctccctat cgttcgcgaa gatacccatc acgcgatctc cataaagcca
     1021 gcaaagcaag acgaagaagc cgatgatgac cgtcacgatt ctggcggccc atcgcgtcgt
     1081 atcgagggcg cgctccactt tttccgcgcc gagattctgt cccacgatcg tggaagcgcc
     1141 catagagacg cccattaacg gcgtgaaggc gatgcctaaa atccttccgg cgatgcctgt
     1201 cgccgcgatg accgcagtcc cgaaggtcga cacgaacttc atcacgatga tttgcgacac
     1261 attgcgcgaa atggtttcga atcccgtggg cagcccgatg gtgagcaatt tcttgtccgt
     1321 ttcccaatgc aagcggaaca gccctttgat ggacggtcga accagattct tttttgagag
     1381 cagataggcg aacccgatga aaaaggcggt cgattgcgaa atgaccgtgg ccaaagccgc
     1441 gccgaatacg cctaaaccga aacccggaat cgacgtgaaa ggaacggtgg cgaacataaa
     1501 cagcggatcc aatatgacgt tcagaacgct cgagaaaagc atcagaacca tcggcgtctt
     1561 ggcgtctccc aaacatcgga ggatggtgtt caccgaatag gaggcgaaca taatcggcaa
     1621 aaggaacagc cggatatacc cgtaatcgaa ggcggccttc gcgacggccg gctcattcgt
     1681 gaagaaggat atcaacgggt taagaaacaa ggccatcaag gctccggcca ctagggccag
     1741 gagcactttg aaagtaatcg tctgttcgat cgcgatctgc gtcgcgggca agtctttccg
     1801 accgaaattc tgtgagatca ccgaaatcga gctctgcccg acgatgtcgt tgagggagcc
     1861 gatcaaccaa aagatactcc cgaagatcgt caccccggcc aatgcttcag atgagatcat
     1921 accgatccaa aagatgtcga cgaggtcgta gaccatctgc agcgaaaacc cgatgatggt
     1981 cggaaggctg agtgagaata tatgcttaat gatactgccg tttgttaaat ctttcattgt
     2041 gaacctccat aatgccccac aaaaaaataa ccgggagaac cattctaccc ggttatatta
     2101 tacccctgcg ccatgcgtca tccgcaatcc cctcgcatta tcgatacctg gccctgggta
     2161 taccggcata gtccgtgttc ttttccttta acacccgttc ggattcgacg ggtacccgac
     2221 gatcctgcct gtatatgaac tgattctgaa ttttaatcat aatctcacct ccctttcctt
     2281 tcttttactc aaacgacttg ttggcgttgc cacgccgcga gccccaaata ggcgatctgg
     2341 cacgcgctct ccacgattct ttcgagacct tttcggttct cgatttctct ccttcgtatt
     2401 agcttcatca gtctcacctc ctttttataa gcgaaaatta tctattctca aacaatttta
     2461 tcgactcgcc ttagggctat gtcaagcgct aaaccgagaa gcgggtgtaa aacttgtgaa
     2521 aaaggtgggc ttaaccccac cttttttggc gaacgtaaaa gcgtcgcgtt cgtttcgtat
     2581 ttagtgatat tgtttcagct cagaagttat tgtccatcca tatcttttta gcttttaacg
     2641 tcgtgagcag gttaaaggac gggtctatcg tgcgtgtgac gttgttgtcg aacaatatct
     2701 ccttgatggt gtcatcctgc aatagaaaga agtaatcccc cagatgaaaa tagccgtaag
     2761 gcagcttggt cgctttcaaa tacttcaaca tctcgtcaat ttgttgatcc aatgctccag
     2821 ctgttctttc gacaaccgta tcgagtaggc gccggctttg atctcgatgc cgtcgtagag
     2881 ttgcgtgaca ccgttgtggt agagatagat gcgttgtacc atgaccaccg cgtcgaaaga
     2941 ggtgaacccc ttcgcgaact tctcttcatt tctcacgacg atcgaggcga aagcgccttc
     3001 cggaatgttc cccacgatct gcagattatt gaagcttagt tcttccggag cgccaaaggc
     3061 gagcgcgacc agaagcgcgc aaacgataag aatagtcctg attctcatat tctccctcct
     3121 ggacagatgt tatctatcaa gacgtaaaaa accgagggcc attgttcgtt caggggtcct
     3181 ttcgttctaa atggggcatt ttagaagatt tttcgttgtg aagcgcgctg gcagcgcaaa
     3241 aagaaagatt acggggtaat gctcaaaaaa aaccgggaaa tttaacgttc ccggtttcaa
     3301 ctacaggggg cggtgttcac ttaacatctt ttccctcgcc tactaaatgt atctcggcca
     3361 ttgaagaacc catatttcat tcattctctt ttccatctct ttctctaact ccgatcgaag
     3421 cagcaagcgt tccaagcgat tcgtctttac gttccgtttg tctttcatct gcgacacctc
     3481 cttcgtttat aaatctcggt taatgccgtc cgggtcgaaa tgggcggtcg ggcgggacag
     3541 ttcgcgcctt gtctgtgtct tcaccgtgtt gggggcgttt aaggccatcc atctcatctt
     3601 ttctcctcct tatcctacgg tttccgtcgg ttctccacga gtcgcgatca aaacagccgt
     3661 tggttctgaa tgaaaaacgg gttctgattg gcctgggcgt ggatgtgggc gatctttttc
     3721 acaatctctt cgtcccgttt gcgccaaaaa cctttcaatc tgttcatgac catccctcct
     3781 ctctttgtgt gtgtacgcaa acatcataac ccttagactt agaacgatgt caagggccaa
     3841 aaaggcctaa tcctgtaaac attttgaaaa gggtggggtt aacccaatga ttattaggaa
     3901 agcgtaggag tgttcagtga gaggcttctt ccgtgttgat tccgaaggtt ttttccacgg
     3961 tcatattctc cccacgattt cgtgtgaccc aagaaaggct ttcgattttg aacgcgtact
     4021 tccacttgta agcgcagagg aaagccgtga tcggtccgat gttttccaac gttaggtctt
     4081 ccatcgccag tgtgacatgg gggatccagc gctccgggtg atagcgctta ttgggtgtga
     4141 cggcgacggg agcaatcgct tcccagatcc ggcggtggaa gcgttccagc tccgcgtcca
     4201 tcataaaagc gatgtaaatc accggacgtt ttccggaaaa gatacccagc ccattggtac
     4261 ggcaccgcaa agggcgcagg cttcgggcca atgaccgaca aaaagcctgg gggtcgtcca
     4321 acagatagcg ttcggcgtag ttccaggtga tgtgcggagt acccgtcagc ttgacacgat
     4381 cgacgccgaa ccgttcgctc aataagtccc agaagtagag gacttcttcg cgaaaaaaag
     4441 gtggtaagat cgagatcagt ccgttcaaaa ctcgaagccc cccggatagg tgttaggaca
     4501 tcccgccatt tgactcgcgc ccgggcggtc aacctatagg gatggtctta ggcgcgcctt
     4561 gtagagacgg cccatatgag gaagtatagc atattgggta cctctactat gggaagggct
     4621 ttgggaacgc cctgggaacg ccaactttca agttggcatg tcattaaaaa agagttcgca
     4681 cggagggcac agagaacacg gagagtgcag catcgcggcg taagcgtttc ttctttccct
     4741 gggaacgcca aactttagtt tggcatggtt tctgggaacg ccaactttta agttggcatg
     4801 tcttgaaaaa cagagttcgc gcagagggca cagagagctc ggagagcaca ggcatcgcgg
     4861 cgtaagcgct ccttcttttc gcgacggcgc gatacccctt tcgttcgttc caaagatcaa
     4921 aaaacaaggt tcgcacagag ggcacagaga acacagagaa cacagagaac acagagaaca
     4981 cagaggaaaa actggcaagg gacagcgttc tctcggaaaa agaaaagata tgctggacta
     5041 aagtctggcg ttcccgtgcg cagcatcgcg gcgtaagcgc ttcttctctt cgctggcgcc
     5101 gacttccagt cggcctttct ctgggaacgc caacttttaa gttggcatgt cccgaaaaac
     5161 agggttcgca cagagggcac agagaagaca gagaaaagat tcttgttaac accctgggaa
     5221 cgccaaactt tagtttggca tagtttcatc ttttcatcgg gcgcgtttcc atgcgcagca
     5281 tcgcgagact tcgtgagttc ccgaagtcag cccgctggtt acccccgcga taacacaagc
     5341 gggcatcgcg gcgtaagcgc cctttctttt cgcgacggcg cgatacccct ttcat
//
LOCUS       DAOPXN010000154         5297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_15558_length_5297_cov_5.854636, whole genome shotgun sequence.
ACCESSION   DAOPXN010000154 DAOPXN010000000
VERSION     DAOPXN010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15558_length_5297_cov_5.854636"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4745)
                     /locus_tag="PKM49_08160"
     CDS             complement(<1..4745)
                     /locus_tag="PKM49_08160"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNY82808.1"
                     /translation="MPSDSLSSDLSTVSRRIEEWKRRLIDGSRNNQLIYWKSSDRSLE
                     IAYPDFSTVLTDVLNRKRLFFFSFSEREEEAFSLAERNDRAFLLERAQSIASRSTNSL
                     VNRNVFFRKPLDLLLRRLISRAKTDYLERGIRVCYLFAGLLHWTDLQTFQPIRSPLFL
                     IPIDLFPPRKTAQNAFSLMLSEDDALINPALEAKLMDSYGFRFPDSIELGTGEEVSHA
                     IDLLSTQFAERNWFIEKTICPGCFSFHKIVLYNDLRQEEEYFSKHPIVRGLAGIDAPE
                     VLDPSPDSKEKGLVSIEQETQSDHWQILDADSSQQAVIEAALKKKNIVLQGPPGTGKS
                     QTISNLISEWIARGWKILFVSEKMAALEVVKKRLEECGLDRFCLELHSQKMNKKTVLQ
                     ELKRSYESLTDPSNVKATVASSEFDFKRLDALKRELDAYPESLHRVIPGIGKSLYEMF
                     GFLAACEDLIDFADRYDFIGADDTFGAWGERFPSVFKRMAEIWDELIQSPALYWRGLR
                     YQQNLFLIRETVEKALSSLAQSISEVCQMLRPLSGALSFQPQSLRDTETTLAAFDLLR
                     DLGPLPTEWLSIDRLERTRQALRRLIQQEESHLATIENHTGRFHLEFLTQSLPTVDPG
                     DLEPFLTLPSAKFEALSFQTQWLQKHLRTVYDIVTSLRREIHADPKPLLYSQCETYGR
                     VLVGIARSEGLPSLNWLLEPQKLAEVRGLIETHRSTLKTLSAYRQYLGEKYTDRIYSL
                     DLPAMIEKMDSFFFTFSKLMKALKDCALSGKLTQQWKEDLKKALFVKTQEVALQNAFA
                     ALDDLPGGLEKKPAEYLDGFLRRYESTAAYEGRTEEKYIAIEILSCLKNDTTFSAKVA
                     DGAQAFLGLEALFGEIRQACGFDLRSVFLAMTVLDTLSAIERYQGALDPLFLFFRTGE
                     GFLKPEAKMPVTTLSELIVMIKEIAEVQAFPGKKAKQLETLERLGTSAQPGLSELRTL
                     SARIERALEAFGLLRSVGFPGEPIETADHPRVKLNQLAFFQTIFTPRDRDRLQEALSL
                     LKTRRQELLALFDPLACQWVKVFGDTVSFSSQMKYLATLKDHLGQIPAWIRFESTIAE
                     EGPSPITRFLALAMNARVQADLLSHTLLKTLYKQAIKREWEENPVLKHFTSRYREEQI
                     EEFRKMDRQSIRITPSRITRRVMERINQTGLPEKEVRILLAESEKRRNVIPVRTLFER
                     IPELLFSLKPCLMMSPLTVSQLIPLKALTFDVVVFDEASQIFTEDAIGALCRARQFIV
                     AGDSKQLPPTNFFQVLNRWTSEEFESPEEALEDSSANYDSVLEEIQRVPNVHRLSLNW
                     HYRSKSESLICFSNKAFYDDRLVTFPEPYHHNPKIGVKHVYVPDGVYDRGKSRQNERE
                     AKKVVELIFQHVSTHPAKSLGVIALSEAQMDAIQDELDKQLVKNEKYRSFFLEDRLNG
                     FFVKNLENVQGDERDVIILSVGYGRDKTGKLTFHFGPINQQGGERRLNVAITRAREKM
                     ILVSSITSADLSASGSASEGLRVLKDYLEYAERGVWESFPTGVSEGVEGEAAFSVDAD
                     VAKTLQEWGLSVYRHVGNGPFRLDIAVLDP"
     gene            5171..>5297
                     /locus_tag="PKM49_08165"
     CDS             5171..>5297
                     /locus_tag="PKM49_08165"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82809.1"
                     /translation="MVKITNPHDKFFKEVMSHIEIARDFLMQYLPPEKARPLDFET"
BASE COUNT         1224 a         1444 c         1370 g         1259 t
ORIGIN      
        1 ggatccagaa ccgcgatatc caatcggaaa gggccgtttc ccacgtggcg atagacggac
       61 aatccccatt cctgaagggt tttggccaca tccgcatcca cgctgaaggc cgcttcgccc
      121 tctacgccct cagagacgcc cgtcggaaag ctttcccaga caccgcgttc cgcgtactcc
      181 aggtagtctt tgaggaccct caacccttcg ctcgcggagc ccgaggcgct caaatcggcg
      241 ctggtgatgg atgagaccag gatcatcttc tccctcgcgc gtgtgatcgc cacgttcagg
      301 cgccgttccc ctccctgctg attgatcgga ccgaaatgga aagtaagctt tccggtttta
      361 tctctcccgt acccgacgct caagatgatc acatctcgtt catcgccttg aacgttttcc
      421 aaatttttca cgaagaatcc gttcaagcgg tcctccaaga aaaagcttct atacttttca
      481 ttcttgacca actgcttgtc cagctcatcc tgaatggcat ccatctgcgc ctcgctcaac
      541 gcgatcacac cgaggctctt ggccgggtgt gtggacacgt gctggaagat cagctcgacg
      601 acctttttcg cttccctttc gttttggcgg ctcttcccgc gatcatatac gccatcggga
      661 acgtagacgt gtttgacccc gatcttcggg ttgtgatggt agggctccgg gaacgtgacg
      721 agccggtcgt cgtaaaaggc cttattagag aagcaaatca acgattccga cttgctccgg
      781 taatgccagt tcaaggagag cctatggaca ttcggaaccc tctgtatctc ctccaaaacg
      841 ctgtcgtagt tggcactcga atcttccaag gcttcctccg gcgactcgaa ctcttcgctg
      901 gtccaacggt tgagtacctg gaagaagttg gtcgggggga gctgtttgga gtcgccggcg
      961 acgataaatt gccgcgcccg acacagagcc ccgatggcgt cttcggtgaa aatctgtgag
     1021 gcttcatcga acacgacgac gtcaaacgtc agcgccttca aggggatcaa ctggctgacg
     1081 gtgagcgggc tcatcatcag gcatggcttc aaagaaaaca ggagttcggg aatacgttcg
     1141 aatagcgttc gaacgggaat gacgtttcgc ctcttttcgg actcggccag gagtatccgg
     1201 acctcctttt ccggcaatcc agtctggttg atcctttcca taacccgtct ggtgatgcgt
     1261 gacggggtga tccgtatact ctgccgatcc atctttcgga attcttctat ctgttcttcc
     1321 ctgtacctcg aggtaaaatg ctttaaaacg gggttctcct cccattcgcg tttaatcgct
     1381 tgcttataga gggttttcaa cagagtgtgt gacagcaggt ccgcctgtac gcgggcgttc
     1441 atcgccaacg ccaagaaacg ggtgatggga gaggggccct cttccgcaat cgtcgactca
     1501 aaccgaatcc aggcgggaat ctgccccagg tggtctttga gtgtcgccag gtacttcatt
     1561 tgagatgaga aggagaccgt gtccccgaac accttgaccc actggcacgc tagagggtcg
     1621 aacaacgcca gaagctcttg ccgacgcgtc tttaaaagag aaagggcttc ttgaagccgg
     1681 tctcggtccc gtggcgtaaa aatcgtctga aaaaacgcca actggttgag cttgacccga
     1741 ggatggtcgg cggtttctat cggctcaccc ggaaagccga cgctgcgtaa aagcccgaac
     1801 gcctcgaggg cccgttcgat tcgggcggac agcgttcgca gctcagaaag gccgggctgg
     1861 gcgctagtcc ccagccgttc gagcgtttct agttgcttcg ctttttttcc gggaaaggct
     1921 tggacttcgg cgatctcttt gatcataaca attagctcgg aaagcgtggt tacgggcatc
     1981 ttggcctccg gtttcaagaa cccttcgccc gttctaaaga agagaaacag cggatccaaa
     2041 gccccctgat agcgctctat cgcgctcagc gtatctaaaa ccgtcatcgc caggaacacg
     2101 gatcgtaaat cgaaaccgca ggcttggcgg atttctccaa agagagcctc gagcccgaga
     2161 aaagcctgag ccccgtcggc tactttcgcg gaaaaagtcg tgtcgttttt tagacaagag
     2221 aggatttcga tcgcgatata cttttcttcc gttcttcctt cataagcagc agtcgattcg
     2281 tagcgccgca gaaacccgtc caggtactct gccggttttt tctcgagacc acccggaaga
     2341 tcatccaacg cggcgaaggc gttctggagc gccacctcct gcgtcttcac gaagagcgcc
     2401 tttttcaagt cctctttcca ttgttgcgtc aatttaccgg acaaggcgca gtccttcaga
     2461 gctttcatca atttcgagaa ggtaaaaaag aaagagtcca tcttttctat catcgcgggc
     2521 aggtccaacg agtagatccg gtccgtatac ttttctccga gatattggcg ataggcgctc
     2581 agagtcttga gcgtggagcg gtgcgtttcg atcagccccc gaacctccgc caatttctga
     2641 ggctccaaaa gccagtttag agagggaagg ccctcgcttc tcgctatccc gaccaaaacg
     2701 cgcccatagg tttcacattg agaatagagc agcggtttcg gatccgcgtg aatctctcgc
     2761 cgtagggaag tgacgatgtc atagaccgtt ctcaaatgct tttgcagcca ctgggtttga
     2821 aaagacagcg cttcgaactt cgcggacgga agtgtgagga agggctccag gtcaccgggg
     2881 tctaccgtcg gtaggctctg ggtcagaaac tcgagatgaa aacgccccgt atgattctcg
     2941 atggtggcga ggtggctctc ttcctgttgt atgagccgcc tcagcgcttg acgcgtacgt
     3001 tccaacctat cgatggacag ccattccgta ggaagggggc ccaggtcccg taagaggtcg
     3061 aaagcggcca gggtagtttc ggtatcgcgt agtgattgtg gttggaagga caacgcgccc
     3121 gaaagtggcc gaagcatctg acagacctcc gaaatgctct gagcaagcga agacagcgcc
     3181 ttttccacgg tctcgcgtat cagaaagaga ttctgttgat atcgtaaacc ccgccaatac
     3241 agcgccggac tttggatcag ctcgtcccaa atctcggcca ttcgcttgaa gaccgaaggg
     3301 aagcgctccc cccaagcgcc gaacgtgtca tcggcaccga tgaaatcgta gcggtcggca
     3361 aagtctatca aatcctcgca ggcggccaaa aacccgaaca tctcgtaaag gcttttcccg
     3421 ataccgggta ttacacggtg taaagactcg gggtaggcgt ccagttcccg cttcaacgcg
     3481 tccagtctct tgaagtcgaa ttccgaggat gctacggtcg ctttcacgtt gctcgggtcg
     3541 gtcaaactct catacgaccg tttcaattct tggaggacgg tctttttatt cattttttgg
     3601 gaatgcaact ccaagcagaa ccgatccaat ccgcattcct caagccgttt tttcacgacc
     3661 tccaacgccg ccatcttttc gctcacgaaa agaatcttcc atccgcgagc gatccattcg
     3721 ctgataaggt tggagatcgt ctggcttttg cctgtcccgg gcggtccctg gagtacgatg
     3781 tttttctttt tcagcgcggc ttcgatcacc gcttgctgcg aactgtccgc gtcgaggatt
     3841 tgccagtggt cgctttgagt ctcttgttct atggaaacca aacctttttc tttcgagtct
     3901 ggagacgggt caaggacctc cggcgcgtca atacccgcga gtcctctcac gatcgggtgt
     3961 ttcgaaaagt actcctcttc ttgtctgaga tcgttgtaaa ggacgatttt atgaaaagag
     4021 aaacaaccgg gacagatcgt tttttcgata aaccaatttc gctccgcaaa ctgggttgac
     4081 aagagatcga tcgcatgcga gacctcctcc cccgtaccca gttcgatcga gtcggggaac
     4141 ctgaacccgt acgagtccat caatttcgcc tctagagcgg gattaatcag tgcatcgtct
     4201 tctgatagca tcaacgaaaa ggcgttttgc gcggtttttc tgggagggaa tagatcaata
     4261 ggaatgagga acaagggaga acgaatcggc tgaaaggttt gcagatcggt ccagtggagc
     4321 agaccggcga acaaataaca aacgcggata ccccgctcca aataatcggt tttcgcgcgg
     4381 gaaatcaacc gacgaagcaa cagatccaac ggttttcgga agaagacatt ccggttgacc
     4441 aaggagttcg tagagcggct tgcgatagac tgcgcgcgtt ccaggaggaa agctcggtca
     4501 ttacgttcgg ctaaagaaaa ggcttcttcc tctctttcag agaagctgaa aaaaaagaga
     4561 cgcttccgat tgaggacatc ggtcaggacg gtggaaaagt ccgggtaagc gatttccaaa
     4621 gaacgatcgg acgatttcca gtagatcagc tgattgtttc gagacccatc gatcaggcgt
     4681 cttttccatt cctctattcg acgactaacg gtcgaaagat cgcttgacag gctatcggac
     4741 ggcatcttta ccccctttta aaggcgagaa tccgccggaa aaatctaaag actatcatac
     4801 cacaagcgcg cgacgcgtcg acaattttcc tgccatccgg acgcgccgga cttccggaag
     4861 cgctctgaaa acgcctctgg gaacgccgaa cttcagtccg gcatttctta tcaagaaata
     4921 acaggggttc acacggaggg cacagagtgc gcagaaggga gattcgcgct gggtccgccc
     4981 tgtgagccat ttttgagcgc cgtgggaacg ccaactttta agttggcagg ttttctacgt
     5041 tttcatttct cctccgtgcc ctctgtgcga aaccttcaaa tatctttttt tcactcccgg
     5101 aaaaccaaac tccccctctt tattagtgta taatatccgt gaacgagttt tctctatagg
     5161 gaggagtcga atggtcaaga tcacaaatcc tcacgataag ttcttcaaag aagtgatgag
     5221 ccacatagaa atcgcaagag actttttgat gcagtaccta cctcccgaga aagcaaggcc
     5281 ccttgatttc gaaacgc
//
LOCUS       DAOPXN010000155         5271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_15672_length_5271_cov_4.690951, whole genome shotgun sequence.
ACCESSION   DAOPXN010000155 DAOPXN010000000
VERSION     DAOPXN010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15672_length_5271_cov_4.690951"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PKM49_08170"
     CDS             <1..242
                     /locus_tag="PKM49_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY82810.1"
                     /translation="KGMEKGLEMGIIKGREEGLEKGMEKGREELLWKLISKKFPKISQ
                     KYFEKLKTLTIEKLDSLGLELIDMKNEEELRKHLM"
     gene            722..1204
                     /locus_tag="PKM49_08175"
     CDS             722..1204
                     /locus_tag="PKM49_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HNY82811.1"
                     /translation="MKTCILYAGKYGTTEHIAKMIQSKLGNDAVLINLTDQKKPDISA
                     AERVVIGSGVYMGKARKEVRHFCKTRASELSSKRTALYLCCMENVEAEVEKYFQKAFP
                     EAVLSKAIVHRRFPGALVRSQKEGWMTRAMFERMRQTNAERKEPVEQWVAAFVETITR
                     "
     gene            1252..2658
                     /locus_tag="PKM49_08180"
     CDS             1252..2658
                     /locus_tag="PKM49_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY82812.1"
                     /translation="MPKKTILGPTFNEMLHPETIPENFRKAALQAKHRDPLDPINFFN
                     ITWKNEKNEIPYFVLPKQITGVPCHIVILYGKDFPTGSHKVGATYSILMEKVIRHEVD
                     PFYHRLVWPSTGNYGIGGAWVGCRMGFDSLVILPELMSQERFDIIHSYGAKVIATAGC
                     ESNVKEIYDKSKELKCADPEKVRVLNQFEEFGNYRFHYYVTGNTLIELAELLKKAGIG
                     NGEISSIVSSMGSSGTIACGDRLKEVFPRCLVVGSEPIQCPTLMQNGYGGHDIQGIGD
                     KHVTWIHNVMNMDAMVAVDDMESKLGLQLLTDPAGKAYLQSIGVFSEEVEKMAAIFGI
                     SGVCNVLSAIKVAKYYDLTEEDTVVTIATDGIDRYHTVMDALNQQFGPMDRTRAAVLF
                     EKTFMGVRTDWLQEGTYEVRKRWHNLKYYTWVEQQGKTVEELNETRKPSFWRKQQDII
                     EETDRLMLEYRTRGGAPV"
     gene            3146..3610
                     /gene="rimP"
                     /locus_tag="PKM49_08185"
     CDS             3146..3610
                     /gene="rimP"
                     /locus_tag="PKM49_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNY82813.1"
                     /translation="MEKKEREEIIRKLVQEEGFELYAYRYTPGKKGARLYIAIDLVER
                     RIDLSDCERVSKRLGEYFDRENWFDSPYTLEVSSPGAERELRTPEDYRRFSGETVKIV
                     LKEPLEKRSVFIGRLSKVSLEERRIEIVERDSTRSFTIGFDQIKKGNLYLEV"
     gene            3615..4652
                     /gene="nusA"
                     /locus_tag="PKM49_08190"
     CDS             3615..4652
                     /gene="nusA"
                     /locus_tag="PKM49_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNY82814.1"
                     /translation="MNIQLINALEQLREDRGISKEEVIDALEKSLKVAYKKSFGEEEN
                     VEVKIDPRNGEITVFRVFEVVKEESEVTDINKIQVDEARKIEHLIAPGDILRQRVNVK
                     KFNRIAAQTAKQVLIQKIRELEKESLFAQYKDMNGKIVTAEVIRETRDGYELRVGKLD
                     TTIPFDRLLPNDRYRNGDLMKVLVESIDRRSRAAAIKVDRVSPQFVQQLLTLEVPELE
                     NGILQIKAIAREAGVRTKIALVSEHPQVDPIGASIGESGGRIASVVRELKGEKIDVFY
                     WSDDPKELIKNALAPADVLEVEIVDPDNRISRVWVPPTQLSLAIGKGGQNARLAAKLT
                     GWKVDIKPLLE"
     gene            4844..>5271
                     /locus_tag="PKM49_08195"
     CDS             4844..>5271
                     /locus_tag="PKM49_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HNY82815.1"
                     /translation="MATKVIIMGAAGRDFHNFNTFFRDNADYKVCAFTATQIPGIEGK
                     VYPAALAGKLYPNGIPIEAEEELKELIKKHAVDQVVLAYSDLPFSYVMEKSALVNSCG
                     ADFVLMGPKRTMLKSKVPVVAVVAIRTGCGKSQTTRRVLD"
BASE COUNT         1436 a         1302 c         1418 g         1115 t
ORIGIN      
        1 aaaagggaat ggaaaaaggc ttagaaatgg ggattataaa aggcagagaa gaaggcttag
       61 aaaaaggaat ggaaaaagga agagaagaat tgctttggaa actaatatcc aaaaagttcc
      121 ctaagatatc gcaaaagtat ttcgaaaagc tgaagaccct tacgatagag aaacttgact
      181 ccctaggact tgagctaatc gatatgaaaa atgaggaaga gttaaggaag catttaatgt
      241 agagagtccg acaaagggct tgaacccgat ttgatatatc atgtgcgcga agttattgcg
      301 gctatttcga gggtcttttt tccctctcgt ttttgtcttt tttcttatta cacccggata
      361 ttatacccac actctaccag gtgttgttga cgggttagtg ccacgaatcc ggcagagtgg
      421 gtcaagctat agggtgcaat caagaagcaa gccagataca atcgcacgat ttttgttcac
      481 gaagtcagcg cggcgtatgc gcttcttttt tttgcgacag cgcgatacgc ttatgtacaa
      541 gccgattaca gcgtagcagg aaggggtaag gagggatcgc ggggtcccgt tggttacccc
      601 gcgatgaacc tcttagttat gtcatctcat agcgcggccc acacaatctg tcggggtgta
      661 gccggattcg tgctataatc ctcccgcgat taccgagatt cgcgaaaagg gggacaatcc
      721 tatgaaaacc tgcatattat atgccggtaa atacggaacg acagaacaca tcgcgaaaat
      781 gatccaaagc aagctgggga acgacgcggt cctgatcaac ctcacagacc agaaaaaacc
      841 tgacatttcc gccgccgagc gcgttgtcat cggcagcggc gtgtatatgg gaaaggcgcg
      901 taaagaggtg cgccactttt gtaaaacccg cgcgagcgaa ctcagtagca agcgcacggc
      961 cctctacctc tgctgtatgg agaacgtgga agcagaggtc gagaagtact ttcagaaagc
     1021 cttccccgag gccgtcctgt cgaaagcgat cgtacaccgt cgatttccgg gggcgctggt
     1081 tcgttctcaa aaagagggat ggatgacgcg cgcgatgttc gagcgtatga gacagaccaa
     1141 tgccgaacgg aaagaacccg tcgaacaatg ggtcgctgcc ttcgtcgaaa cgatcacgag
     1201 atgaatactg accataagac cttaggaggt gaaggcgcaa cgcctttttg aatgcctaaa
     1261 aaaacgattc ttggacccac attcaacgag atgctacatc cggaaaccat cccggaaaac
     1321 ttcagaaaag ccgcgcttca ggctaagcac cgcgacccgt tggatccgat caatttcttc
     1381 aacatcacgt ggaagaacga gaagaacgag atcccctact tcgtcctgcc gaaacagatc
     1441 accggagtgc cctgtcatat cgttatcctt tacggaaagg acttccccac cggttctcac
     1501 aaagttgggg caacctattc gatcttgatg gaaaaggtca tacgtcacga ggtcgatcct
     1561 ttctatcatc ggctcgtatg gccatccacc ggaaattacg ggatcggagg cgcctgggtc
     1621 gggtgtcgga tggggttcga ctccctcgtg atccttccgg aactgatgag ccaagagcgg
     1681 ttcgacatca tccattcgta cggcgcgaag gtgatcgcca ccgccgggtg cgagtccaat
     1741 gtgaaagaaa tctacgataa atcgaaagaa ttgaaatgtg cagaccccga aaaagtgagg
     1801 gtgctcaacc aattcgagga gttcggcaac taccggtttc actactatgt caccggtaac
     1861 acgctgatag aactggcgga actgctcaaa aaggccggaa tcggaaacgg agagatctct
     1921 tctatcgtct cttcgatggg ttcctctggc acaattgcct gcggtgatcg gctgaaagaa
     1981 gtcttcccgc gttgcttagt ggtgggttcc gagcccatac aatgtcccac gttgatgcag
     2041 aacgggtacg gcggccacga tatacaagga atcggcgaca aacacgtcac ttggatccac
     2101 aacgtgatga atatggacgc gatggtcgcc gtcgacgata tggaatccaa actcggactg
     2161 cagctgctca ccgaccccgc cggaaaagcc tatctccaat ccatcggcgt cttttccgaa
     2221 gaggtcgaaa agatggccgc catcttcggc atctccggcg tatgcaacgt tctcagcgca
     2281 atcaaagtgg ccaaatacta cgacctcacc gaagaagaca cagtcgtgac gatcgcgacc
     2341 gatggcatcg accgctatca cactgtgatg gacgcgttga atcagcagtt cggccctatg
     2401 gatcggaccc gggccgccgt acttttcgag aaaaccttta tgggagtgcg cactgactgg
     2461 ctgcaagagg ggacttacga ggttcgcaaa cggtggcata acctcaagta ctatacctgg
     2521 gtggagcaac agggtaaaac cgtcgaagag ctcaacgaaa cgaggaagcc aagtttttgg
     2581 cgaaaacagc aagacataat cgaggaaacc gatcgcctca tgctcgaata ccgcacaaga
     2641 ggaggggcgc ccgtttagtt ctatttccgc ctggtttctg catctgaaca tctgggcgga
     2701 ttcaacggat tccttagctg cgtttagagt ttagatccta tgcccgtact ctatacttgg
     2761 tgcgggtata atattgaggc gcaccaaaaa aaagacaaga ggaggagatc cgccttttgt
     2821 cctttttatg ccgcgatgac tacgaggatc attccggggt aagtggtttt tacgaccccg
     2881 cgatccgcct tttatgtttt ttacgccgca atcaacacgc ataaaaacgt atcgcgcagt
     2941 cgcataaaaa agggcgcttt cgccgcgatg actacgtgga cgaaaaaccg tgcgattggt
     3001 tctggccttg tttcttggtc tcacctcata acttgaccca ctctatactt gacgtcgacg
     3061 tatttatttg gtacaattag ggaaacaata tacgaagttt gtttcgaggg ctgcgccctc
     3121 gaaacttttt actggtgaaa agaggatgga gaagaaagag agagaagaga tcatccgaaa
     3181 gctcgttcag gaggaaggtt ttgagctata cgcctatcga tatactcccg gaaagaaggg
     3241 agcgcggctc tacatcgcga tcgacctcgt ggagcgaagg atcgatctgt ccgattgcga
     3301 aagggtatcc aaacgtttag gcgagtattt tgatcgcgaa aactggttcg attcccccta
     3361 caccttggaa gtctcttctc cgggagccga acgagagctc agaaccccgg aggattaccg
     3421 gcgcttctcg ggagaaacgg taaaaatcgt tttgaaagaa cccctcgaaa agcgaagcgt
     3481 gttcatcggc cgcctttcca aggtatctct cgaggagcgg cggatagaga tcgtagagag
     3541 ggactccacc cgcagcttca ccatcggatt cgaccagata aaaaagggga acctttacct
     3601 ggaggtatga tgggatgaac atccaactga ttaacgctct ggaacaactg cgggaagacc
     3661 gtggaatctc gaaagaagag gtgatcgacg cgctggaaaa gtcgctcaag gtcgcctata
     3721 aaaagtcctt cggggaggag gaaaacgtcg aggtgaagat agacccacgc aacggggaaa
     3781 tcacggtctt tcgggttttt gaggtggtga aagaggagtc ggaagtgacc gacatcaaca
     3841 aaatccaggt ggatgaagct cggaagatcg aacacttgat cgctccgggt gacatactcc
     3901 ggcaaagggt gaacgttaag aaattcaacc gtatcgcggc tcaaacggca aaacaggtgc
     3961 tcatacaaaa aatccgggag ctggaaaaag agagtctgtt cgcacaatat aaagatatga
     4021 acgggaaaat cgtcaccgca gaggtcatcc gggagacccg cgacgggtat gaattacgcg
     4081 tagggaaact ggatacgacc atccctttcg atcgtttgtt accgaatgac cggtatagaa
     4141 acggcgacct catgaaagtc ctggtcgagt cgatcgatcg ccggagtaga gccgccgcga
     4201 tcaaggtaga ccgggtgtcc ccccaattcg tccaacaact gctgaccttg gaagtgccgg
     4261 aactggaaaa cgggatcctt caaatcaagg ccatagctcg cgaagcaggc gtgcggacca
     4321 agatcgcgct ggtttccgaa cacccgcagg tagacccgat cggcgccagt attggagaaa
     4381 gcggcggtcg tatcgcctcg gtcgtacgag aattgaaagg agaaaagatc gacgtcttct
     4441 attggtccga cgacccgaaa gagctcatca aaaacgcgtt ggcgcccgca gacgtgttgg
     4501 aggtggagat cgtcgacccg gacaaccgca tctcacgggt gtgggtgcct ccgacgcagc
     4561 tgtcgctagc gatcggaaag ggcggccaaa atgcccgatt ggccgcaaaa ttgacgggct
     4621 ggaaagtgga tatcaaaccg cttctggaat gaagatggtg gcggacgcat accggagcgc
     4681 gcagacgtat gccccagcat tctcgagata gccgcgccat ttgagcaacc tgaccggctt
     4741 agccgccgcg ttcttcctga ccaccgtttg tgatttcgtc gttccctttg ttatactcga
     4801 ccgagaaaaa tcgtgatgtt ttcatatctt tggaggtgta tgaatggcaa cgaaagttat
     4861 cattatgggc gccgccgggc gcgatttcca caacttcaac acgtttttta gagacaacgc
     4921 ggactataag gtctgcgcct tcacagccac ccaaatcccg ggcatcgaag gaaaggtcta
     4981 ccccgcggct ctagccggaa aactctatcc caacggcatt ccgatagagg cggaagagga
     5041 actcaaagaa ctcatcaaga aacacgcggt ggaccaagtg gttctagcct acagcgacct
     5101 tcccttttct tatgtgatgg aaaagtccgc gttagtcaac tcgtgcggag ccgacttcgt
     5161 cctgatgggc cccaagcgca ccatgctcaa atccaaagta cccgtggtcg cggtggtcgc
     5221 gatcagaacc ggctgcggta aaagccaaac cacacgcaga gtcctcgaca t
//
LOCUS       DAOPXN010000156         5224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_15876_length_5224_cov_4.875411, whole genome shotgun sequence.
ACCESSION   DAOPXN010000156 DAOPXN010000000
VERSION     DAOPXN010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15876_length_5224_cov_4.875411"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..1400
                     /locus_tag="PKM49_08200"
     CDS             426..1400
                     /locus_tag="PKM49_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNY82816.1"
                     /translation="MNFYEQQKKNLIKTTLLVFVMLAVVTVAGIALDYSFGTGFLITG
                     VLIVITLIQTVVSLSSGAGIVLKSVNAKEVNPNTRDLEEKQLLNIVEELSIASGTPKP
                     KVYVMRDASINAFATGNKPENAYVCVTTGLLQHLNREETEGVLAHELAHIKNRDILLM
                     TMVSALAGGVLLLSLIAFRGGLTLFQTSGRSKKSKDSGSIAGIALAMLVTAGVMFLVG
                     QLSRFMTFAISRKREFLADATGAEMTRNPMGLSLALRKIYNLPVITKSANSATAHLFI
                     SEPKKRSLAERRDLLANLLSTHPPLVDRIAVLEGKDPLTIQRELTQKG"
     gene            complement(1406..2353)
                     /locus_tag="PKM49_08205"
     CDS             complement(1406..2353)
                     /locus_tag="PKM49_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007040255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNY82817.1"
                     /translation="MNEQKDTQKKYRLVTRSDFDGLVCAILLRELNLIEDILFVHPKD
                     MQDGKIPITDSDITTNLPFVEGVHLAFDHHLSEMTRVGGKKPNHIIDPDAPSAARVVY
                     NYYGGKATFKTIPDEMMRAVDKGDSAQFSREDILNPTGWDLMNFLMDARTGLGRFRNF
                     RISNYQLMMDLIGYCKNHSISQILELPDVKERVDFYFAEETLFREQLLRCSKVYQNLV
                     VLNLKEEETIHAGNRFMIYALFPECNISIHQMWGLKKQNTVFAVGKSILNRTSKTNIG
                     ALMLKYGGGGHENAGTCQVPHEEAQTVLKELIEQINKDG"
     gene            3096..>5224
                     /locus_tag="PKM49_08210"
     CDS             3096..>5224
                     /locus_tag="PKM49_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNY82818.1"
                     /translation="MRSNLVHCPLNCASPFGYSYNRVILDEAGVPVDYEYVETNRAFD
                     RMTKVAQPFLARRRFSEFAAEWMDEPDEWLRWCGEVALHGEAKEAVRYFSKTDRWYKV
                     QIFPSETLHFFTTFFDITQEMKIQQELERFFLGNPGPSCILDSEGRFLTVNEAWTKIL
                     GVTREEILNSRVIDLIHPEDRQQTREALRHLQEKKQVSDLITRWRTKAGTYRYFLWNA
                     TLEGPRIYATARDVTANKEMGATLREQRERYRLMVEAAKAGILVVQDDRIVFCNPAAL
                     EIVAASQGEIINTPFSGLVYPEDRGREQTDHKKAFLMGTSEQGYLFRILNKSGEVRWV
                     DVSEDTILWDGRPAMVKFLKDITFRVLAQKELAAAEEKYRTLLDNSLTLTYSIKLNGE
                     ITYISPICLELLGYHSKEMTGRDIRDFIYHEDYPRLKNLRKEALENHLLKSDREYRVI
                     HRDGSLRWLRCVVGLIFDENGLPTSFVGNAIDTTERKRMEQSLVEAKEQAESANKAKS
                     TFLANMSHEIRTPLNSIIGFSDLLKNSSLSPAQRLYAENTHTAAEALLNIINDILDFS
                     KIEAGKMEFNVQKTDLIYLLEESIDIIKFQASKKNLELLLNIQQDIPRFAYLDGVRLK
                     QVLMNLLSNAVKFTEHGEVELSVTYLPQNRAEGSYTFSVRDTGIGVEEGYQDKLFDVF
                     TQGDESITRKYGGTGLGLSISGLIVEK"
BASE COUNT         1333 a         1271 c         1357 g         1263 t
ORIGIN      
        1 agtccagcaa cccgctcgaa taaaatcgta tcgcgccgtc gcaaaaagaa agggcgctta
       61 ccccgcgatg ctgcgcttta gaacgccgca ctttagtgcg gcatgtattc tatgttatct
      121 cggtgccctc tgtgctctct gtgcgaaccc tgttttttgt gaaacaagcc aacttaaaag
      181 ttggcgttcc cagaaaggca aagacatgcc gaactgtagt tcggcgtttc caggcaaaac
      241 gcgcgcgtcc ggtgaattca acaaccctgt tcgcctgatg accgcgccca cccagtcacg
      301 atctttcctt ttccagcgtg atataatatt cttggaaggg gttatttcat cgagccgttc
      361 aagtcggagc accaaaggag agatgcaaga accgcttgac ccaaaaggag ggttcggttg
      421 tggtgatgaa tttttacgaa cagcagaaaa agaaccttat aaagaccacc ctgttggtct
      481 tcgtgatgct ggcagtcgtg acggtggccg gaatagcttt ggattacagc tttggaactg
      541 ggtttttgat cacgggtgtt ctcatcgtga tcacgttgat ccaaacggtc gtttccctct
      601 cttcaggcgc tgggatcgta ctcaagtctg tgaatgcgaa agaagtgaat cccaacaccc
      661 gagatttgga agaaaagcaa cttttgaata tcgtcgaaga gttgtccatt gcttcgggga
      721 caccgaagcc gaaggtgtac gtgatgcgcg acgcgtcgat caacgccttc gcgacgggta
      781 ataagccgga aaacgcctat gtttgcgtta cgaccggttt actccaacat ctcaaccgcg
      841 aagagaccga aggcgtgttg gctcacgagc tggcgcacat caagaatcgc gatatactgt
      901 tgatgacgat ggtgagcgcg cttgcgggag gggtgctgct cctctccctg atcgcgttcc
      961 gcggagggct gacgcttttc caaacgagcg ggaggtccaa gaaatcgaaa gacagcggga
     1021 gcatcgccgg aatcgctttg gcgatgctgg tgacggcagg ggtgatgttc ttggtcgggc
     1081 aactcagtcg gtttatgacc tttgcgatct ccagaaagcg agagttcctg gcggacgcga
     1141 ccggcgcgga gatgacccgg aatccgatgg ggttaagttt ggccctgcgg aagatataca
     1201 atcttccggt catcacaaaa tcggccaaca gcgcgaccgc gcatctcttt atctccgagc
     1261 cgaagaagag gagcttggcc gaacggcgtg atctgctcgc gaacctactg agcacccatc
     1321 cgcctctggt cgaccgcatc gcggtcttgg aaggtaaaga tcctctaacc atccaaagag
     1381 aactcactca aaagggatag aacggttacc cgtctttgtt gatctgttcg atgagctcct
     1441 tgagcaccgt ctgtgcttct tcgtgcggta cctggcaggt tcccgcgttt tcgtggccgc
     1501 cacccccgta cttcagcatc aacgcgccga tattcgtctt tgaggtccga ttgaggatgg
     1561 atttacccac ggcgaagacg gtgttctgct tctttaaccc ccacatctgg tgaatcgaga
     1621 tgttgcattc ggggaagagc gcgtaaatca taaaccggtt gcccgcgtgg atggtctcct
     1681 cttctttcag attcaacacc acgaggtttt ggtagacctt cgagcagcgt aaaagctgtt
     1741 ctcggaagag cgtttcttca gcaaaataga agtcaacgcg ctctttcaca tctggtagtt
     1801 ccaaaatctg tgagattgag tgatttttgc aatacccaat gaggtccatc atcaactggt
     1861 agttagagat tcgaaaattg cgaaagcgcc cgagaccagt ccgagcgtcc atcaaaaagt
     1921 tcatgaggtc ccagccggtt gggttgagga tatcttctct cgaaaattgc gcggaatctc
     1981 ccttatccac agcccgcatc atttcgtctg ggattgtctt aaaggttgct tttcctccat
     2041 agtagttata gacgactcga gcagctgaag gcgcgtcggg atcgatgatg tggttcggtt
     2101 tctttccccc gacgcgggtc atttcgctca agtggtggtc aaaagctaag tgcacgccct
     2161 ccacaaaagg caggttcgtc gtgatatccg agtctgtgat cggaattttc ccgtcttgca
     2221 tatcctttgg atggacgaaa agaatatctt caatcagatt caactctcgg agcaggatag
     2281 cgcacacgag accgtcaaaa tcgcttcgcg tgaccaaacg atattttttt tgagtatcct
     2341 tttgttcatt cataaagcgc acctcttctg ttatatttcc tggaagtcga ccctctgaaa
     2401 attataccac attcgtttag ctccttactc ttacgtgtgg gtcaagttat ggggtgcgct
     2461 caagaaacaa tgtcagaatc gtccgtacga ttttaagttc ttgtagtcat cgcggcgtaa
     2521 gcgcttcttc ttctcgcgac ggcgcgatac ccttttgcat atattttgaa acacagggtt
     2581 cgcacagagg gcacagagaa cacagagaaa agactgaaga aaactcgtgg ggccgacttc
     2641 cagtcggcct gtctctctgg gaaggctctg ggaacgccaa cttttaagtt ggcggatttt
     2701 tcgcggagtc gtaaactacg cttaacacgt gatgaacctt gtagttatta cgtcgtaggc
     2761 gtttaagggt tctcggaagc ttccagaaaa acaaaaacca tgccgaacta aagttcggcg
     2821 ttcccagagg agcatttcca aaacagcaaa acctcgaacc atgccgaact aaagttcagc
     2881 gttcccaggg cggatcacaa gaataaaagg cggaccgcgg ggtcgaaaaa accacatacc
     2941 ctgcgatgaa cctctgagtt atgccacctc ataactttga cccgcaccga taatgaaagg
     3001 atcggggagt tcatggtaca attactcaat caaaaaagac ctcctgcgtc gctttgaggc
     3061 ctttaagaga gaatggttga ttgggaggtt gagagatgcg atcgaatctc gtgcattgcc
     3121 cccttaattg cgcgtctcca tttggttatt cctataaccg ggtgatcctt gacgaagcgg
     3181 gagtcccggt ggattatgag tacgtcgaaa ccaatcgggc cttcgatcga atgacgaagg
     3241 tcgcacaacc tttcctagca cgccgacggt tttctgaatt cgcggccgaa tggatggacg
     3301 agccggacga atggttgagg tggtgtggag aggtcgccct ccacggtgag gcaaaagaag
     3361 ccgttcggta cttttccaaa accgatcgct ggtataaggt ccagattttt ccgagcgaga
     3421 ccctgcactt tttcacgacc tttttcgaca tcactcaaga aatgaaaatc cagcaagagc
     3481 tcgaacggtt cttcttgggc aatcccggcc catcctgcat actcgattcg gaagggcgct
     3541 ttttaactgt caacgaggct tggacgaaga ttctcggagt aacccgcgaa gaaatcctaa
     3601 acagtagagt cattgatttg attcacccgg aggaccggca acaaactcgt gaagcccttc
     3661 gccacctcca ggaaaagaag caggtttcgg acttaatcac acgatggcga acgaaagcgg
     3721 gaacctatcg gtatttcctc tggaacgcca cgttggaagg cccacgcatc tatgcgacag
     3781 cgcgagatgt gacggcgaac aaggagatgg gcgccacttt acgagaacag cgcgagcgat
     3841 acagattgat ggttgaagcc gctaaagcag ggattttagt ggttcaagac gatcggattg
     3901 tcttttgcaa ccctgcggca ttggagatcg tggccgcctc ccagggagaa ataatcaata
     3961 cgcccttttc aggtttagtc tatcccgagg accgtgggcg ggaacagacc gatcacaaaa
     4021 aagcctttct gatgggaacc tcagagcaag gctacctttt tagaatcctc aataagagtg
     4081 gagaagttcg ctgggtcgat gtgagtgagg atacgatctt atgggatggt cgaccggcta
     4141 tggtgaaatt tctgaaagac atcaccttcc gggttttggc gcaaaaagaa ctggccgccg
     4201 ctgaagagaa atatcgaacc ctgttagaca acagtttaac gttgacttat tccatcaaat
     4261 taaacggaga gatcacctat atctcaccga tttgcttgga gttattgggg tatcattcca
     4321 aagagatgac gggccgtgat attcgagatt tcatctatca cgaagactat ccgagattaa
     4381 aaaacttgag aaaagaggct ctggaaaacc accttctgaa aagtgataga gaataccgtg
     4441 tgatacatcg cgacggctct ttacgttggc tccgttgcgt ggttggtctc atcttcgacg
     4501 aaaatgggct ccctacctct ttcgtaggaa acgccataga cacgacggag cgcaaacgga
     4561 tggagcaatc gcttgtggag gcaaaggagc aagcagaatc tgcgaacaag gctaagtcta
     4621 ccttcctcgc caatatgagc cacgagatac gcacccccct gaactcgatc ataggctttt
     4681 ccgacttgtt gaaaaactcc tcgctctccc cggcgcagcg tttgtacgcg gagaacaccc
     4741 acacggcggc cgaggcgttg ttgaatatta tcaacgatat tttggatttt tcgaagatcg
     4801 aagcggggaa gatggaattc aacgtgcaga aaaccgacct gatctatctt cttgaggaat
     4861 cgatcgatat aataaagttc caagcttcca aaaagaattt ggaattgctg ttaaatatac
     4921 agcaggatat ccctcgtttt gcgtacttgg acggtgtgcg cctcaaacag gtgttgatga
     4981 acctgttaag caacgcggtc aaatttactg aacacggaga ggtggagctg agcgtgacct
     5041 atctcccgca aaatcgagcg gagggctcct acacattctc ggtgcgagat accggtatcg
     5101 gtgtcgagga ggggtaccag gacaaactgt ttgatgtctt tacgcaagga gatgaatcca
     5161 tcacgcgcaa gtatggtgga acaggcctcg gattgtccat ctccggattg atcgtcgaaa
     5221 agat
//
LOCUS       DAOPXN010000157         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16010_length_5195_cov_4.704864, whole genome shotgun sequence.
ACCESSION   DAOPXN010000157 DAOPXN010000000
VERSION     DAOPXN010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16010_length_5195_cov_4.704864"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..533
                     /locus_tag="PKM49_08215"
     CDS             66..533
                     /locus_tag="PKM49_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY82819.1"
                     /translation="MDQKTDLFEERLQAANASYRDGDIKAALKAYARLMETYPEEPLC
                     YYNMALAFIEKEDYTMAKKLFEQALKLGFISSKIYIGLGFCALKLSQFEEAATFFAGV
                     PEEAEEYIEGLIGRVYASVLSHQTGNIPPLLAELKGRNVWNQELELLSKRSRY"
     gene            complement(1074..2120)
                     /locus_tag="PKM49_08220"
     CDS             complement(1074..2120)
                     /locus_tag="PKM49_08220"
                     /inference="COORDINATES: protein motif:HMM:NF027565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4899 domain-containing protein"
                     /protein_id="HNY82820.1"
                     /translation="MFFDFFALKIRAESKASDEVFFGYIFGKTGEKVEYRFICIPRRF
                     SKQVSVDLNSPLSTFKEFLNHHYQELFTKTPEVMELTTEFMRYLEMQIKKTGGREAFS
                     VRYSKVIADGDFDVVKMELLNLFQNWTTSLDIDLVVDTSTYEELTSSYLKDIDFGDFA
                     QSLSSRNDLVTLPEIYPIVDPLDGASIDEYDIGDTLFCTLLGFTAIDQEKLLREEFPD
                     HFDAEGNNTVPFEGVIISKEILPLISKNFVLVKVQIGKSFQAKSMVLRSIRLMYDPNK
                     MRKRLQSIKQDADTHSISVADAMSKYKKVREESSKKLLDSGKVRINDFVLTLIFLMLI
                     FGLVAIILYYFFLT"
     gene            complement(2133..3419)
                     /locus_tag="PKM49_08225"
     CDS             complement(2133..3419)
                     /locus_tag="PKM49_08225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNY82821.1"
                     /translation="MASGITAMILAGGQGTRLGVLTEDIAKPAVPFGGKYRIIDFTLS
                     NCVNSGIFRVGVLTQYKPHLLNAHIGIGRPWDLDRMGGGVTILQPYTYETGNIWFRGT
                     ADAIYENFYFIEKNDPKYVLILSGDHIYAMDYMDMLNYHVSKHAFATCACMNVPLAET
                     SRYGIMVTDLQQKIVEFQEKPLHARSTLASLGIYIFNWDYLKALLKQDAAKEESTHDF
                     GNDIIPEMVKNNADFYAYEFEGYWRDVGTLPSYWEANMELIQPLPPLSLYNPSWRFYT
                     RTRERPPAYFGPEAVVLDTLVSEGSEVFGVVEKSVIFQSVTIAKGASIKNSVVLTGSV
                     IEEGAEISNAIIAENATIGKGVRIGNKTFAPNREDPKVYNSLITTIGYNASVPDNTTI
                     GSNCVVHSGVSKSDFEKGAYESGESVWKKEQRSNNT"
     gene            complement(3431..4549)
                     /gene="glgD"
                     /locus_tag="PKM49_08230"
     CDS             complement(3431..4549)
                     /gene="glgD"
                     /locus_tag="PKM49_08230"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HNY82822.1"
                     /translation="MKVLGLIFAGARGESLGLLTTKRASAGMPVAGKYRAIDFTLSNM
                     IASNITRVGVLTQYNPRSLIDHLGSGKEWDLDRKHGGLMLLQPYMDIHSSYWYRGTAD
                     AIHQNMTFLRRSEEDYVLVTSGDHIYNFNFSELFNYHFAKGSDITFMYKSLDDTYKPC
                     EYGILQVDDENRIYSFEEKPTHPVGNKASLGVYFMNKYMLMDMLYNTVPQAEGYDFLL
                     DVLIPNMQRLRYFGYEFTGYWRNIKKGIQEYYDVNMSLLDRKVRQELFYDGVTIHTKI
                     KDLPPPKCTGTAFVKNSIVADGCVISGRVLNSVISRGVKVKAGALVENSILLQDTIVE
                     EGSTVKFSILDKECLVRSERRIIGRKEDILVFEKGSVI"
BASE COUNT         1260 a         1316 c         1266 g         1353 t
ORIGIN      
        1 cagtttggcg caatgagggg ccgcggcgga atagcgatac ccgttgaaaa cgtgaaggag
       61 ggtaggtgga tcaaaaaacc gatttattcg aggaaagact gcaagcggcc aacgcttcgt
      121 atcgagatgg agatatcaaa gcggctttga aagcttacgc gcgcttgatg gaaacctatc
      181 ctgaagaacc gctgtgctat tacaatatgg cattggcatt catcgagaag gaagactaca
      241 cgatggcgaa aaaactcttc gaacaagccc tgaagttggg tttcatttct tcgaagattt
      301 acatcggctt ggggttttgc gcccttaagc tctcgcaatt tgaggaagcc gccacgtttt
      361 tcgctggggt acccgaagag gccgaagaat acatcgaagg cctcatcggt cgggtttacg
      421 cttcggtatt gtctcatcag accggaaaca ttcctcccct cttagccgaa ctaaagggca
      481 ggaatgtgtg gaatcaggaa ttggagttgc tttcgaaacg gtcccgttac tgaaaccctc
      541 gcgtctcgca tacgtttctt cacacaggac agatcaatta tcgcgataaa ctccacaaat
      601 agcttctcgc gtaaggatga tcctgaggaa acctgcgttc ttccgagaag tgtaaccgct
      661 ctttctgttt cgcgaaagcc ttccggtcga tatcctcgct ccggtaaagt gacagggcgt
      721 caaacggctc tttcacctct tcaacttcgt ccccacgatc aacattcggt gatacgagaa
      781 aagggcgtcg atgagttcgt caaaccaagt tcaatcgcgg gaatctgccg ttctcttcgt
      841 tcctcccttg attcacttgt ttgagcgttt ccgagtaatt ggggaccgtt gtggtgtcaa
      901 aggcccggta tttcttttct aaccggcgct tcccttgttt ctggaagaac agcgtcctcc
      961 cactttcctc tatcgattgg aacaggtcgc tgctcctttg ggaggggagg tctccctcat
     1021 acgggcggat atggtatctc ttttcgcatc atgaggctat ccaagaaatc aggttacgtg
     1081 aggaaaaaat aatataggat gatggcgacc aacccgaaga tgagcataag gaatatgaga
     1141 gtcagaacga aatcgttgat ccgcaccttt ccgctatcca gcaacttttt tgaagattcc
     1201 tcgcgaactt ttttatactt cgacatcgcg tcggcaacgg atatcgagtg cgtatcggca
     1261 tcttgtttta tactttgaag gcgtttgcgc atcttgttcg ggtcgtacat caaacgaatc
     1321 gagcgtaaga ccatagactt tgcttggaaa gacttcccta tttggacctt gaccaacacg
     1381 aagtttttag aaatcaaagg gaggatttct ttggagatga tgaccccttc gaagggcacg
     1441 gtgttgttcc cttccgcgtc gaaatgatcg gggaactctt ctctgagaag tttttcctga
     1501 tcgatggcgg tgaaccccag aagggtgcag aacaacgtgt ctccgatgtc gtactcgtct
     1561 atcgaagcgc catccaacgg gtccacgatc gggtagatct ccggaagggt caccagatca
     1621 tttctcgatg agagcgattg cgcgaaatct ccaaaatcga tatcttttaa ataactggag
     1681 gtcaattctt cgtaagtgga cgtgtccact acgagatcga tatccaacga agtggtccaa
     1741 ttctggaaga ggttcagcaa ctccatcttc acgacgtcga agtctccgtc cgcgataacc
     1801 ttcgaatacc gtacggagaa ggcttcgcgc ccgccagttt ttttgatctg catttctaag
     1861 tatcgcataa actcggtggt gagttccatc acttcgggag ttttcgtgaa aagctcctga
     1921 taatgatggt tgagaaactc tttgaaagtg gaaagcgggc tgttcaagtc cacactgacc
     1981 tgtttcgaga aacgccgggg tatacagatg aaacggtact cgactttttc acccgtttta
     2041 ccgaaaatat aaccgaaaaa cacctcgtcc gaggctttgc tttcagcgcg tatcttgaga
     2101 gcgaaaaaat cgaaaaacat cggcttcccc gtttaggtgt tattgctccg ctgttctttt
     2161 ttccacacgg attctccgct ttcgtaagcc cctttctcga agtccgattt cgaaacgccg
     2221 gaatgaacga cgcagttgct cccgatggtc gtgttatccg gaaccgacgc gttataccca
     2281 atcgtggtga tcaaagagtt gtacaccttc gggtcttccc ggttcggcgc gaaggtttta
     2341 ttcccgatcc gaacgccctt accgattgtg gcgttctccg cgatgatcgc gttggagatc
     2401 tccgctccct cctcgattac gcttcccgtt aagacgacgg agtttttaat actggcccct
     2461 ttcgctatcg tgacgctttg gaaaataacc gatttttcca cgacaccgaa gacctctgac
     2521 ccttcgctga cgagggtgtc gaggacgacc gcctccgggc cgaagtatgc gggaggacgc
     2581 tctctcgtcc tggtgtaaaa cctccacgag ggattgtaca aactgagcgg gggtagaggt
     2641 tgtatcaact ccatattcgc ttcccaatag gaaggcaacg tcccgacgtc cctccaatac
     2701 ccttcgaatt cgtaggcata gaaatcggcg ttgttcttga ccatttccgg aatgatatcg
     2761 tttccgaaat cgtgagtgga ctcttcttta gccgcgtctt gtttcaacaa cgccttgagg
     2821 tagtcccagt tgaagatgta gattcctaac gaggctaagg tacttctggc gtggagaggc
     2881 ttctcctgaa attccacgat tttttgctgc aaatccgtca ccatgatacc ataacggctc
     2941 gtctcagcca gggggacgtt catacaggcg caggttgcga acgcgtgctt ggaaacgtgg
     3001 tagttgagca tatccatata gtccatcgcg taaatatgat caccggaaag gatgagcacg
     3061 tatttcggat cgttcttttc gatgaaatag aagttttcgt agatggcgtc tgcggtgccc
     3121 cgaaaccata tatttcccgt ttcgtaggtg tagggttgca ggatcgtcac tccgccgccc
     3181 attctatcca aatcccaggg tctcccgatc ccgatatgcg cgttgaggag gtgcggtttg
     3241 tattgcgtga gcaccccaac cctaaagatc cccgaattca cgcagttgct caaggtgaag
     3301 tcaatgatgc ggtacttccc tccaaaagga acggccggct tcgcgatatc ctccgtcaga
     3361 acccctaaac gggtgccctg accaccggct agtatcattg ctgtgattcc ggatgccatc
     3421 attcccctcc ttatataacg ctacccttct cgaaaacaag gatatcttct tttcggccga
     3481 tgatccttcg ctcacttcga accaggcatt ctttgtccaa aatcgaaaac ttgacggtac
     3541 tcccttcctc cacgatcgta tcttgcaaga ggatcgagtt ttccaccaac gccccggctt
     3601 tgaccttcac gccgcgcgat atgaccgaat tgagaacccg gcctgagatc acgcatccgt
     3661 ccgcgacgat ggaattcttc acaaaggccg tccccgtaca tttcggaggc ggaaggtctt
     3721 tgatcttcgt atggatcgtg acgccatcat agaacagttc ttggcgaact tttctatcca
     3781 atagactcat attgacgtcg taatactctt gaatcccttt tttgatgttt cgccaatacc
     3841 cggtgaattc atacccaaaa taccgtagcc gttgcatgtt tggaatgagc acgtccagta
     3901 aaaagtcgta cccttccgct tggggtacgg tattgtacag catatccatc aacatatact
     3961 tgttcataaa ataaacgccc aaagacgctt tgttccccac cgggtgggtc ggtttctctt
     4021 cgaacgagta gatgcgattt tcatcatcca cctgcaaaat gccgtattcg cacggcttat
     4081 aggtgtcatc gagagatttg tacataaaag tgatatccga acctttcgcg aaatggtagt
     4141 tgaacaattc cgagaagttg aagttgtaga tatggtcccc ggaggtgacg agaacgtagt
     4201 cctcttcact ccggcgaaga aaagtcatat tctgatggat cgcgtcggcg gtgccccgat
     4261 accagtaaga gctgtgaata tccatatagg gctgcagtaa catcaacccg ccgtgttttc
     4321 tatccaaatc ccattctttt ccggacccca aatgatcgat caggctcctg ggattgtatt
     4381 gggtcaaaac cccgaccctc gtgatgttcg aagcgatcat attcgataaa gtgaagtcga
     4441 tcgcccggta tttcccagcg acaggcatac cggcactcgc tcttttcgtc gtcaataggc
     4501 ctagcgactc tcctcttgct cctgcgaata ttagccctaa gactttcacg tcactccccc
     4561 caatccggcg tattcaatag agattatacc acgttctttt tctttgcgct gcttcacgat
     4621 gcgagttccg aaaagaaatc gcgtttggta gatttaggag gggtgtggga acgccaactt
     4681 ttacgttggt atgtcttaaa agcgaaagac aggagagagc gcagcagaaa acaggtaggg
     4741 ccgacttcca gtcgggctgt ccctcattag tatcgcgaga cttcgtgggc ttccgaagtc
     4801 agcccgttgg ttacccagcg atgaacccca cacggtcatc gcggcgtaag cgccccttct
     4861 cttcgcggcg gcgcgatacg cttttgtaaa tgtcttgaaa aacagggttc gcacagaggg
     4921 cacagagaac acagagaaaa gacagaagga gactcgtagg gccgacttcc agtcaacctg
     4981 ttcctctggg aaggccctgg gaacgccgaa ctttaattca atatgggttc ctgggaacgc
     5041 cgaactttag ttcggcatgt tctattgttt tatcgatgtc cgattattta ggagtaaaat
     5101 cagacgtagg ctcgagagaa gtcgccgtga aaaaagaggg ggccaatcgg cttcgtaaaa
     5161 ggcatagggc ggatcgcggg ggcgaaaaac tactt
//
LOCUS       DAOPXN010000158         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16023_length_5192_cov_4.923886, whole genome shotgun sequence.
ACCESSION   DAOPXN010000158 DAOPXN010000000
VERSION     DAOPXN010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16023_length_5192_cov_4.923886"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="PKM49_08235"
     CDS             <1..1352
                     /locus_tag="PKM49_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82823.1"
                     /translation="TETFLARVIPQIRAAVGGNLTEEELQERGLEELAKEVKKEKARL
                     NDFYIRYEDNQTKMDRNAIEIYAGARDLLSRCLDLAPYYGRSHFYMAALMVRPERKLI
                     LFQQKAEAKDPLDPLRVFFTEVTEERKHIAPAYRLFPLERDFEWLESKVREGLSLEQM
                     EAWVDIHNWYDIQSYRDSLSYFHTSFLGFDERNAYRQMGKSYYSLHAMLRNLIVGYSQ
                     LLREHPQWEKELRPLMERHQQEAIAAFEEFKRAYDRAIYLLPGNWQMFPEWESIYSEY
                     ISLLLKNESVQTVYPKIRAIANLRVQACEWMRQVQRVGIPDDIAVFYAQLSQTFLDSR
                     LYQETLYVIDEGLNTLTKAYRWNKEDLENNPYLTEEDKTRIRGFLTAYESLYRNRSLF
                     IDQVLEVYAQAQVNGTFEQLWLDDWKEHLFVDRKWPEANYTDIINLLKRAKAERAPL"
     gene            complement(1805..4207)
                     /locus_tag="PKM49_08240"
     CDS             complement(1805..4207)
                     /locus_tag="PKM49_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82824.1"
                     /translation="MSMKKSTGLVLLLGVIAVMFVVSCVKQVETKPEAPVNLRISFLA
                     GIVGVELRWDEVAGATGYEITLTKTEASGSRAVLGPYSTAKTYYLVYRDDVGAGSFSW
                     TVKAKNGAGDSGASVESHFTLDPDPEPDPVYGLTLTLVETPDLASSRDGIPEIHIWGA
                     TTSIPGAGARVPGSPVNLNYLAGRIVYEEVSDAPRETMSVQLMVERKGSGVAKRWPDS
                     STEFLLNGDGELLFGVNDLGQNYDPNRLAPGEYLFWARAAWDKNMQSTKKPFNIVAST
                     DLVEVSLDVSNQKTAYPGKVCGIFPEAQIDYDISVSGAALFDELRYTFVIGQLGVDAT
                     GNVSKSATFTGRTAYATSVTYATECTKFATVTVDGTLTRVLWEDATKRASETKLGVVG
                     AFEKKHSFVLDLADPTALLSLELPAGFSASAPASLTSATLTFLASDTKCLQPYAEKIN
                     FKVGVEKGSGSWSESFAFFTEEVVLGEGSPWDNAVTIDATYTATKTSATNPDEAEAVL
                     GFAIPIMDYATVTATMSVHDCCCEECTIKPGKCDNLNNALRHATEVSTQFIVDNVFFS
                     KLLEDEQVFVPERFLGDADNPLIPASPTQATLTVKLADALWSSAQWGAITWVFHDAQG
                     NVFNSISYAPTIKATELITGECSGYATKTQVTFVGTLTADPAATRTEKALTLVATALD
                     ATGNEFVVKLPFLFDTLPPTLTHFTGFRDKVNNHSWVEFKFDQKAESAELAIVVGENT
                     FSYDFNDAVPIAGIPNAFRLPTGITLPYNLAITLKATATDLAGNIGFSSRTCTVSLSE
                     KR"
     gene            complement(4498..>5192)
                     /locus_tag="PKM49_08245"
     CDS             complement(4498..>5192)
                     /locus_tag="PKM49_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82825.1"
                     /translation="DDVLKVDNKSLGKVNGCYLIPASPAVATVTVKLADANWEPPNPL
                     VLHYNFPGPQTINVTRASTAATLAVCSGYATKTQATYIATFTGYAKDNSVPVATETNI
                     TYTGTATDNAGNEFLLSECFRLDTMPPTLTHFAAFRNPSVTNGSYIEFSFHEEPVSAA
                     LTLEITGQGTFTYTLAQATSVPPHLYTYSLLTGISLSEGATVTLTAKAKDLAGNEGTS
                     SWETKVSPSAPR"
BASE COUNT         1173 a         1447 c         1457 g         1115 t
ORIGIN      
        1 cgacggagac gttcctggcg cgggtgatcc cgcagatacg cgccgccgtc ggcgggaacc
       61 tcaccgagga agagctgcag gagcgcggct tggaagagct tgcgaaagaa gtcaagaaag
      121 aaaaggcgag attgaacgat ttctacatac gctacgaaga caaccaaacg aaaatggacc
      181 ggaacgcgat agagatatac gccggcgccc gcgacctatt gtcgcgctgt ttggatttgg
      241 ctccgtatta tgggaggagc cacttctata tggccgcgct gatggtgcgc ccagagcgga
      301 aactgatcct gttccagcaa aaagcggaag ccaaagaccc cttagacccg ttgcgtgttt
      361 ttttcacaga ggtgacggag gagcgtaaac acatcgcgcc cgcctaccgt ctctttccgc
      421 tggagaggga cttcgagtgg ctggaatcga aggtccggga gggcctttcc ttggaacaga
      481 tggaggcgtg ggtcgacatc cacaactggt acgacataca gagttatcgg gactcgcttt
      541 cctacttcca tacctccttc cttggattcg atgaaagaaa cgcctaccgg cagatgggca
      601 agtcgtacta ctcgctccac gccatgcttc gaaacctgat cgtcggttac tctcaactgc
      661 ttcgagaaca cccgcaatgg gaaaaagagc tccggccgtt gatggagagg caccagcagg
      721 aagccatcgc ggcgtttgaa gagttcaagc gcgcctacga tcgcgcgatc tacctgttac
      781 ccggaaactg gcagatgttc ccggagtggg aatcgatcta ttccgagtac attagcctgc
      841 tcctgaaaaa cgaatcggtt caaaccgttt acccgaagat ccgcgcgatc gccaacctgc
      901 gcgttcaagc ctgcgaatgg atgcgccagg tccagcgcgt cggcatcccc gacgacatcg
      961 cggtgtttta cgcgcaattg tctcaaactt ttttggactc tcggttatat caggagaccc
     1021 tgtacgtcat cgacgagggg ttgaacacac tgacgaaagc ttatcgctgg aacaaagagg
     1081 acctcgaaaa caacccctac ctgacggaag aggataaaac gcgcatccgc ggttttttga
     1141 ccgcttacga gtcgttgtac cgcaaccgca gcctgttcat tgaccaggtg ttggaggtct
     1201 acgcgcaagc ccaagtcaac ggtactttcg aacaactgtg gctcgacgat tggaaagaac
     1261 acctgttcgt cgatcgtaaa tggccggagg cgaactacac cgatatcatc aacttgctga
     1321 aacgcgcgaa agccgaacgg gcgcccttat aaacgcgccg aacttagcgg atcgcctggg
     1381 agcgccgcat tgccgccctc atcgcggggt aaccaacggg accccgcgat acgcgcttgt
     1441 gatcgcctgg gaacgccttc gggacgcgca cttctggaag cgcctgggaa cgccgaactt
     1501 tagttcggca tgtttttgct ttcgggtacg tcgcacttcg tggatcatcg cggggtaacc
     1561 agcgggctga cttcggaagc ccacgaagtc tcgcgatact gatgtaacca cccgattcac
     1621 ctgtccaaaa gcgtatcgcg ccgtcgcaaa aagaaaaagc gcttacgccg cgagtaccgt
     1681 cgagacgccg cgataatcca caaagcccgt caccctccgg cagcccaaca aaagaaacag
     1741 gggcgatgga tcgcccctgt ctgctgatac cagcacacgc gttgaaaggt gcctggataa
     1801 cgatttaccg cttttcagaa agggatacgg tgcaggttct cgaagagaag cctatgttac
     1861 cggccaggtc cgtagccgtg gctttgagcg ttatcgctaa gttgtaaggc aaggtgattc
     1921 cggtcggtaa tcggaaggcg ttcggtatcc ccgcgatcgg aaccgcgtca ttgaaatcgt
     1981 agctgaaggt gttctctccg actacgatgg ccagctctgc gctctcagcc ttctggtcga
     2041 acttgaattc cacccatgaa tggttgttga ctttatctcg gaaaccggtg aaatgcgtga
     2101 gcgtcggagg taacgtgtca aataggaagg gtagtttgac aacgaactcg ttgccggtgg
     2161 cgtccaaagc ggtcgccacg agggtcaggg ccttctcggt tcgcgtagcc gccggatcgg
     2221 ccgttaaggt ccccacgaag gtcacctggg ttttcgtcgc gtaccccgaa cactctccgg
     2281 tgatgagttc ggtggctttg atcgtgggag cgtaactgat agaattgaat acgttgcctt
     2341 gggcgtcgtg gaagacccaa gtgatggcgc cccactgcgc cgaactccat aacgcgtcgg
     2401 cgagcttaac cgtcaacgtc gcctgcgtcg gggaagcggg aatgagagga ttatccgcgt
     2461 cgccgaggaa gcgttccggt acgaagacct gctcgtcttc taagagcttg gagaagaaga
     2521 cgttgtccac gatgaactgg gttgacactt cggtcgcgtg tcggagcgca ttgttcaagt
     2581 tatcgcactt acccggtttg atcgtgcatt cctcacaaca gcagtcgtgc acagacatcg
     2641 tcgcggtgac cgtcgcgtaa tccataatcg ggatggcaaa ccccaaaacg gcctcggctt
     2701 catccggatt cgttgcggag gttttggtcg cggtgtaagt cgcgtcgatc gtgaccgcgt
     2761 tgtcccaagg gctaccctct ccaagaacga cctcttcggt gaaaaaagcg aaagactcgg
     2821 accagctgcc gcttcccttt tcaacgccca ccttgaaatt gatcttctcc gcgtagggtt
     2881 ggagacattt cgtgtcgctc gccaggaagg tgagagtggc gctcgtcagc gaagccggcg
     2941 cggatgccga gaatcccgcc ggtaattcca aagacaggag ggcggtcgga tcggccaaat
     3001 ccaacacgaa actgtgcttt ttctcgaagg ctcccactac acccagcttc gtttccgaag
     3061 cgcgcttggt cgcgtcctcc cataagaccc gggtcaaagt gccgtctacg gtcaccgtcg
     3121 cgaatttggt gcattccgtc gcataggtca cactcgtcgc gtatgcggtg cgcccggtga
     3181 aggtggcgga cttggacaca tttccggtag cgtctacgcc caactgaccg atcacgaagg
     3241 tatatcgcag ctcatcgaac aaagcggctc cggacacgga tatgtcgtaa tcgatttgcg
     3301 cttcgggaaa gataccgcag actttccccg gataggcggt cttctggttt gacacatcca
     3361 gcgaaacttc gacgaggtcc gtggatgcga cgatgttaaa gggtttcttg gtgctctgca
     3421 tgttcttgtc ccacgccgcg cgggcccaaa acaggtactc tccgggcgcg agccggttgg
     3481 gatcataatt ctgcccgagg tcgttcaccc cgaacagcaa ttctccgtcc ccgttcaaaa
     3541 ggaattcggt gctcgaatca ggccaacgct tcgcgacgcc gctccctttc ctctccacca
     3601 tgagctgaac ggacatagtc tcgcggggcg catcagagac ttcctcatag acgatacggc
     3661 cggccaaata gtttaggttt acgggtgacc ccggaacccg cgcgcccgcg ccggggatag
     3721 acgttgtagc cccccagatg tggatttcgg gtataccatc gcgcgaggaa gccaaatccg
     3781 gggtttcgac caaggtgagg gtcaatccgt agaccgggtc cggttcgggg tctgggtcca
     3841 aggtgaagtg actttcgaca ctcgcgccgg agtcccccgc cccgttcttc gccttcacgg
     3901 tccaagagaa gctccccgca cccacgtcgt cgcgatacac gaggtagtaa gttttggccg
     3961 tggaataagg ccccaacact gctcggctac ctgaagcctc tgttttcgtt aacgtgattt
     4021 cgtagccggt agcgcccgca acttcgtccc atcgcagctc tacaccgacg atccccgcga
     4081 ggaacgaaat ccggagattc acgggcgcct ccggtttggt ttccacctgt tttacacaac
     4141 tgacgacgaa catcacggcg atcacgccca acaacagaac caaaccggta ctcttcttca
     4201 tactcatact gtccccccac tttcatataa aaatagattc aagatagacg ttttcctcga
     4261 cgcctggaat gcggttcgtt cgggtagagc gcgtcgagcc tgttctctag aagccggtcg
     4321 ctaccgtggg gacgcgccga cgggaaagat tatagcactt cgcgagcgcg gcggcaaaac
     4381 aaaggcgaaa aacgtatcaa actcgccggg taaagggtct ggcggtacca gccgcggtgt
     4441 ggaaagcaaa aaaaacggct gcctgagcag ccgttttggt ttcgtgatcc ttttcgccta
     4501 tcgaggagcg gacggcgata ccttcgtttc ccaggaactg gtaccttcgt ttccggccaa
     4561 atctttcgcc ttcgccgtaa gcgtgacagt cgcgccctcc gataaactta tgcctgtgag
     4621 aaggctatag gtatacagat gtggtggtac agaagttgcc tgagcgagcg tgtaggtgaa
     4681 ggttccctga ccagtgattt ccaacgttaa ggccgcagag accggttctt cgtggaaaga
     4741 gaattcgata taagaaccat tagtgacaga aggatttcta aacgcggcga aatgcgtcaa
     4801 ggtcggcggc atcgtgtcaa gcctgaagca ttcactgagt aggaattcgt taccggcgtt
     4861 gtcggtggcg gtacctgtgt acgtaatatt cgtttctgtc gctaccggga cactattatc
     4921 tttggcatac cccgtgaacg tcgcgatata cgtcgcctgc gtcttcgtcg cgtagccgga
     4981 acacactgcg agagtcgccg cagtagaagc gcgtgtaacg ttaattgttt gcggcccagg
     5041 gaagttgtaa tgtagaacta gaggattagg cggttcccaa ttcgcgtcgg cgagtttaac
     5101 cgtgaccgtc gctacagctg gtgacgcggg gattaaatag cacccattca ccttccctaa
     5161 ggacttgtta tcaaccttca aaacatcatc tg
//
LOCUS       DAOPXN010000159         5183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16063_length_5183_cov_4.803822, whole genome shotgun sequence.
ACCESSION   DAOPXN010000159 DAOPXN010000000
VERSION     DAOPXN010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16063_length_5183_cov_4.803822"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PKM49_08250"
     CDS             complement(<1..464)
                     /locus_tag="PKM49_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY82826.1"
                     /translation="MRRKSLSAFLLLLLGITLLTSISGCLSRNRPTFAPPTIELTSPA
                     SGSTDLATPVKLEWEAAPGAQTNAGKRDGVTITEYEVNYKKTDGTGTTQTATTVDKSY
                     SLTDLAYGTQYTWWVVAKQSDGQRKKSDEWVFSTQAAQYDTPEIALKSPEDGA"
     gene            complement(735..2810)
                     /locus_tag="PKM49_08255"
     CDS             complement(735..2810)
                     /locus_tag="PKM49_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82827.1"
                     /translation="MKGKRISVVSFLLVGLIVISGCLCRVIITPPIIALVEPADEATD
                     QPTALILTWEATPGALKSNKTRLVTITDYLIYFSRIDEDYGDPETTPDKQVEKTGLAY
                     GATYKWKVTAVQSDGQTASEERTFSTQETPVPSQTIHRYDSTGRYQNSYDRLSDAVNE
                     ANDKDRIEVLGGTVLDNETQQVTISSKEVSIRSSNAAPFVLDMQQRDRVLMLTDGASV
                     TLRGAIITGGEANADGGGVYVESGAFSAIDTTITSNFTLSFGGGVCVLGTFNAVNTKI
                     ESNRAFWSGGGVCVIGTFNAVDTTIESNTGAFGGGVYVESGVFNATDTTVLSNTANWG
                     GGGVCVYGGVFNDHNSTIESNLSYENGGGVVVGGTFNSIGTTIASNTALELGGGVHVY
                     ANGTFNAMSSSLLSNIANFGGGMYLVGTCNATNTTMASNRADTHGGGTYISGTESPGA
                     FESGNLNAAHTAITSNRAQRGGGVYVSYGAFDAFDTTVASNTANGEYAQGGGVCLEKW
                     GIFNARNTVIASNKSHGVYASGGGVGVYDGAFNATGTTISSNEADGQAVTCGGGVYVG
                     GGAFNSFNTTINANTANGENASGGGVYIDTGGTFNALNTTVSSNAAELFGGGIYWNYE
                     DGGRIYTSGNPWKQNDVTPPDDQPNDFSVDTNGEIKTGTEYSDFHPARVFGNTAEKFP
                     NTDQMMVDK"
     gene            complement(3812..3887)
                     /locus_tag="PKM49_08260"
     tRNA            complement(3812..3887)
                     /locus_tag="PKM49_08260"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3852..3854),aa:Phe,seq:gaa)
     gene            complement(3895..3969)
                     /locus_tag="PKM49_08265"
     tRNA            complement(3895..3969)
                     /locus_tag="PKM49_08265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3935..3937),aa:Val,seq:tac)
     gene            complement(3979..4054)
                     /locus_tag="PKM49_08270"
     tRNA            complement(3979..4054)
                     /locus_tag="PKM49_08270"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4018..4020),aa:Glu,seq:ttc)
     gene            4364..4969
                     /locus_tag="PKM49_08275"
     CDS             4364..4969
                     /locus_tag="PKM49_08275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY82828.1"
                     /translation="MSKNEFDEFSKELIAMAQSGGLTLGDVDIDQFESINTRYGRAGG
                     DRVLAHVEQLLKSCLPEGFFVRRFGDEFYVYSSNCSLEAMFMELEEVRAKIAAHKVPY
                     DGHEIEVQVSGSVGEFGRTADNVVKLLNLCSEGLMLAKKQGRNRMVFAPGSREQKMVL
                     KSSYYLSSQLEGLSKLSAKTQKSESALLREAIDDLLRKYGL"
BASE COUNT         1127 a         1401 c         1383 g         1272 t
ORIGIN      
        1 gcgccatcct cgggactctt gagcgcgatc tccggggtat cgtattgagc ggcctgtgta
       61 gaaaagaccc actcgtcgct cttcttgcgt tgcccgtccg attgtttggc cacgacccac
      121 cacgtgtatt gagtcccgta tgcaagatcc gtcaggctat atgatttgtc aactgtcgtc
      181 gccgtttggg ttgtacccgt gccatcggtt tttttgtaat taacctcata ctctgtgatg
      241 gtcactccgt cgcgtttccc cgcattcgtt tgagcgcccg gtgccgcttc ccattccagt
      301 ttcaccggtg tcgccaagtc cgtcgatccg cttgcggggc tcgtgagctc tatcgtcggt
      361 ggcgcaaacg ttggcctatt gcgggagaga caccccgaaa tcgaagtcaa caaggtaatt
      421 cctaacagta gaagtaaaaa cgccgaaagt gactttcttc tcatttagta ccctccttcg
      481 tgcagacgag aaaacatcaa aaagaaagta aaggaaagaa ggaacacggt atccacgacc
      541 ttcaagccct tatcgttaaa gttagcctta tggcgaccgt atgcggttgc gtcatcgagc
      601 gcatcttggg aatattttac catagcaacg aaagaaaaca aagagactga ctggaaacac
      661 cctcgaagcg ccgcaagcct gtgggcgccc tgggaagcct tgggtgctcc cagaagttcg
      721 gtacgctttg agagttattt atccaccatc atctgatcgg tgttgggaaa cttctccgcc
      781 gtattcccga agactcgggc gggatggaaa tcactatact ctgtgccggt ctttatttcc
      841 ccattcgtgt cgactgagaa atcgttcggc tggtcatcgg ggggagtgac gtcattctgt
      901 ttccaaggat taccggatgt gtagattctt cctccgtcct cgtagttcca ataaatcccg
      961 ccaccaaaca actccgcagc gttcgaggag accgttgtgt tgagagcgtt gaaagtccct
     1021 cccgtatcga tgtatacacc gcccccactg gcgttttcgc cattcgcggt gttcgcgttg
     1081 attgttgtgt tgaaagagtt gaaagcgccc ccacctacgt acacaccgcc gccgcaggtt
     1141 actgcttgtc catccgcttc attcgaggat atagtcgtgc ccgtggcgtt gaaggcgccg
     1201 tcatagacac ctaccccgcc cccactggcg tacactccgt gcgatttgtt cgaggcgatc
     1261 accgtgttgc gagcgttgaa aattccccat ttttcgaggc atactccacc gccttgggca
     1321 tactctccgt tagccgtgtt agaagcgacc gtcgtgtcaa aggcatcgaa agccccatac
     1381 gagacgtaca cgccgccgcc tctttgagcc ctatttgagg tgatcgccgt atgcgccgcg
     1441 ttgaggttac cgctctcaaa cgcgccagga ctttccgttc cgctgatgta tgtccctcca
     1501 ccatgcgtgt ccgcacggtt tgaggccatc gttgtgttcg tggcgttgca ggttccgacg
     1561 aggtacatcc ctccaccgaa gttcgcgatg ttcgagagga gcgacgaact cattgcgttg
     1621 aaggtcccat tcgcatagac gtgtacccct cccccgagtt ctaacgccgt gttcgatgcg
     1681 atcgttgtgc ctattgagtt gaatgtcccg cctacgacca cacccccgcc gttttcgtat
     1741 gagaggtttg attcgatcgt cgagttgtgg tcattgaaca ccccgccata gacgcacaca
     1801 cctccacccc cccagtttgc ggtgttcgag aggaccgtcg tgtcggtggc gttaaagacc
     1861 ccgctttcga cgtacacgcc gccgccaaat gctcctgtgt tcgattctat cgtcgtatcc
     1921 accgcgttga acgtcccgat gacacacacc cctcccccac tccagaaagc ccggttcgat
     1981 tcgatcttcg tgtttacggc gttgaaagtt ccgaggacac aaacgccgcc accgaaagag
     2041 agcgtgaagt tcgaagtaat cgttgtgtcg atggcgctaa acgcgccgct ttcgacgtac
     2101 acgccgcctc cgtctgcgtt tgcctctccg ccggtgatga tcgccccgcg tagtgtaacc
     2161 gaggctccgt cggtgagcat caaaacacga tctctctgct gcatatccaa gacgaacggc
     2221 gccgcgttgc tcgagcggat cgaaacttct ttgctcgaga tcgtgacctg ctgagtctcg
     2281 ttgtccaaaa ccgttccgcc taacacttcg atacggtcct tgtcgtttgc ctcgttgacc
     2341 gcgtcgctga gcctgtcgta actgttttgg tatcgacctg tcgagtcgta gcggtgaatg
     2401 gtctgagatg gaaccggagt ctcttgcgtg gaaaaggttc gctcctcgct cgccgtctgc
     2461 ccgtccgatt gtaccgccgt gaccttccac ttgtaggtgg cgccgtatgc cagacccgtc
     2521 ttctccacct gtttgtcggg cgtggtttcc gggtcgccgt agtcttcgtc tatccgtgag
     2581 aaatagatga ggtagtcggt tatcgttacc agccgcgttt tattgctttt caatgcgccc
     2641 ggcgtcgctt cccaggtgag gataagggcg gttggttgat cggttgcctc gtctgcaggt
     2701 tcaacgagcg cgatgattgg cggggtaatg atcactcgac aaagacatcc cgaaatgacg
     2761 attagcccga ccaataggaa cgaaaccaca gaaatgcgtt tccctttcaa ggaaagacct
     2821 ccttcataca gcgagagggt gggagtgtca gggtcattcg aggtgaacgc aatgtctgcg
     2881 ccccccaagc tctcgccgct ctaccttcgt tttggtgaat aagaaacaag ttcgcaggag
     2941 ttcgtgtgaa ctgagtttac catgccgagg agataaaaaa aagtgctggt gtgggtcaag
     3001 ctatggggtg taatcgagaa acaagaccag ggtcgatcgc acggtttttt atccgtgtag
     3061 tcatctgggc gtaagcgccc tttcttttcg cgacggcgcg ataccggttt tcgttcgttc
     3121 caaagttcaa aaaacagggt tcgcacagag ggcacggaga acacaacgga aaaccgggca
     3181 agagaccggg ctctctcgga aagaaacatg cccgactaaa gtccagcgtt cccagtgctt
     3241 tcacaaaaaa aggttcgcac ggaggtcaca gagggcgcgg agagaacaat ggaccatgcc
     3301 acactaaagt gtggcgttcc caggctttcc aagggtggat cgcgaggtcg taaaaaccac
     3361 ttaccccgcg ataaccccac agatccattg cggcgtctta aaaaacaaaa tgcgtatcgc
     3421 gagactacgt gggcttccga agtcagcccg ctggttaccc cgcgatgaac ctcttagtta
     3481 tgtcaccgca taaattgtcc cacacagctg tctccgcgtg tatgcgcaat ttccagaatg
     3541 tagaaaaccg agtctttgta gtttgacata accaaatcga tttttcatcg ctttgtcaag
     3601 aaaacggcgg ggttcacgct attccttcgc ctgaaaaatg gtacaacttc tgcctgcttt
     3661 ctgtggtcct cgaggttttt tcaggggtct ttctttgaac gttaaagagg ttttttggtc
     3721 atttacaaca aaaaagaccc caaggggggg tctttttgtt ttctgctttc taacacaccg
     3781 acgccaaaat cggcgtgttg gcttattagg ctggtgccga ggggcagaat cgaactgccg
     3841 acacacggat tttcagtccg ctgctctacc gactgagcta cctcggcgat aacttggtgg
     3901 gagcggcagg ttttgaacct gcgacctcct gcttgtaagg caggcgctct cccgctgagc
     3961 tacgcgccca aacgaagttg gcgctcccgc gggggtttga accccggttt ccgccttgaa
     4021 agggcgatgt cctagaccgc tggacgacgg gagcataaat ctgcgctata tcttactata
     4081 tctccccgcg tttgtcaaga caccaccggt gtttaatcgc aaagacatgc cggactgaag
     4141 tccggcgttc ccaggcgtgc gacgttccag ggtgctgtcg gaaaggcgta aaagcgtgaa
     4201 cttaagacgt gccggactga attccggtgt tcccaggcgt gcggcgctcg caaggcgctc
     4261 ccaagacgct cccaggtgct tacaggaaaa ttctaagggg ttgacacgag tatatatata
     4321 tgatagtata tatacatata tcaacataaa gggagggagg gcgatgtcga agaacgagtt
     4381 cgatgagttc tctaaagagc tgatagctat ggcgcagtcg ggagggttga cccttgggga
     4441 tgtggacatc gatcagttcg aatcgatcaa tactcgttac gggagggccg gaggggaccg
     4501 ggtgctcgct catgtcgagc agctgctgaa gtcctgtttg ccggaaggat ttttcgtccg
     4561 ccgattcgga gacgagttct acgtgtattc ttcaaactgt tctctcgaag cgatgtttat
     4621 ggagctggaa gaggtgcgcg cgaagatcgc cgcgcataag gtaccctatg atgggcatga
     4681 gatcgaagtg caagtcagcg gttccgtcgg agagttcggt aggaccgccg ataacgtcgt
     4741 caagctgctg aacctttgct ccgaggggtt gatgctcgcc aagaaacagg ggagaaaccg
     4801 tatggttttc gcccccggct cgagagagca gaagatggtc ctcaaatcga gttattactt
     4861 gtcttcccaa ttggaagggc tttcgaagct ttcggctaaa acccaaaagt cggaatcggc
     4921 gttactccga gaagccatcg acgatctact tcgcaagtac ggtttgtgaa cgtcaaacgg
     4981 tcgccctact ccatcaacgt tacggagtgg gcgatcgtct cactccgtgt caagccgaag
     5041 atttcgaaaa cgcttctcga accaatcgtg gggtatcagg cttcagacgc ttttgatgta
     5101 attgttcgat gtctcaaaga tcgtcgcgcg aagccgccat tctcccttta tcgtttctgc
     5161 tgttccacgc cgttgatgat gag
//
LOCUS       DAOPXN010000160         5153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16192_length_5153_cov_5.917026, whole genome shotgun sequence.
ACCESSION   DAOPXN010000160 DAOPXN010000000
VERSION     DAOPXN010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16192_length_5153_cov_5.917026"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="PKM49_08280"
     CDS             <1..2153
                     /locus_tag="PKM49_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82829.1"
                     /translation="RLRKWNVENKNLENVYTRHRRLVNSAAFSPDGRFLASGSSDNTL
                     ILWNIAAGEVERDVIAHTDWVTSLCFSPNGHFLITTSIDKYIKVLAVERGYQLRNIGP
                     FDSAVWSSVYVSEEKVLFGLASGNLSLWNVETAKEIRLEPQAHSAAIRALRFSKKTGQ
                     AYSASNDGMIRIWDTENLNLIATFLMVANGEWISYMPLGKYVSSLNAFVRHDFFVKDG
                     DKEYAFDQYRDFLQKVEHLPIGDIFGPEVILADHTVTPRQTTLTLEISDPGIVEEVNE
                     FGIQYPIHEKRTSLPITFDIWKRPSSTLEVQAVDSFGNVSQTTFGLTFAGFRFFLKEE
                     HPPLPKNALVSLDAIVDNLFVVLWQGEKVTVPKDKVTQTPYAPEISIQIQGEPLVMNQ
                     TESPQLRVKITIADILGVKSGKVGAWQEIRFREPVRRYELDQIIPLELGTNIITAIAT
                     NIEDISQESSLTVFRIEREPPQIVISPVPSQIYEDQFPLGIRVSDNHMVAFVSVNGRE
                     EPVMQSHKDLTVTVPVAIGENRVVVEAVDVFKNAARTLVRFNGVRTMYAKNDGIKVSD
                     AQGNLLHILMKGDPVTVFSHTPPQTWVELSRGVRGVVASSDLQREVVDILPPGFWDVQ
                     AKLDGDSVLVTGFVYDDKEVVSLFVAGQQVQALTPVNITIPKDGAKKVYAFSITLKPA
                     AVGALPDEIKPIELAVYDTVGFFSTLIVVPKVYR"
     gene            2173..2964
                     /locus_tag="PKM49_08285"
     CDS             2173..2964
                     /locus_tag="PKM49_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNY82830.1"
                     /translation="MEWIVPILSTLVGFVIGSFLNVVIFRFNQPSLTVFKPSRSICLN
                     CKKPIPWYDNIPVLSFVFLQGRCRYCQTKLSWRYPFIEALTAAGFLVNSLTFAFTLPF
                     PLALVGADVLAGALIVLFFTDLDTMLISDANTAFVIAGCALLYLDQGLRIDHSIFLIN
                     LITALIAFSLIYILRLLFKIFGNKEAMGMVDIVLISALALALGPMKVNLAIIVACFTG
                     ILAALFFRKKLRDKIPFGPFIAVGGYTALIAGDAFLNLIGFMPYF"
     gene            3470..4786
                     /locus_tag="PKM49_08290"
     CDS             3470..4786
                     /locus_tag="PKM49_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNY82831.1"
                     /translation="MKKMMWATLFACLYFATLLAGSDPKATAKTYLESFFKRDFETMW
                     TLQDETMRRVFGKQAMEQSSKTIEVAFGAFEAILSIEESEVQGYHVFLFYTKMQKSYA
                     QITVSVDSQNLVAGFYAKPAPSPSALTPPYADTQRFTEEEVVFGSEQWPVYGALTLPK
                     IDAPYPLVILVGGSGPTDRDSTIGPNKPFRDLAYGLSSAGVAVLRYDKRPATHGERML
                     LSETSERGAVWDEYLEDLEAAIAYASELESIGPIYLAGHSLGGQAAAYVAIESECLSG
                     LILLAAPARRFAQISIDQNRYFFSSSGLPQEQLDAVVSFFQSVLNRELPPEIEIQPGL
                     TVGYLYDWDRYQTMDSLEKVRLPVIALQGEEDFQATMDGDFLPLKEAFGDSSRFTFKS
                     FPGLNHLFMRTETDVFHTVDEYQTEGFVAYEVIDTLLSWIRQTRSE"
     gene            4783..>5153
                     /locus_tag="PKM49_08295"
     CDS             4783..>5153
                     /locus_tag="PKM49_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY82832.1"
                     /translation="MITYTNDLSGVKAETFVGFFEGWVQKPTPEQHLEILRHSAFCWL
                     AFAGTRCVGFINAISDGFFYAAIPLLEVVPEYRGKGIGAELIRRMRQTLKDFYAVDLI
                     CDVDPDFVTGTPSKRYKQRKR"
BASE COUNT         1263 a         1349 c         1356 g         1185 t
ORIGIN      
        1 gaaggcttcg aaaatggaat gtggagaaca agaacttgga aaacgtctac acccggcatc
       61 gacggttggt gaactcggcc gccttctcgc cggacggtcg gtttctcgcc tctgggtcat
      121 cagacaacac gctcatactg tggaatatcg ccgccgggga ggtggagcgc gatgtcatcg
      181 cgcacacgga ttgggtgacc agtctttgtt tctccccgaa cggacatttt ttaatcacga
      241 ccagcatcga caagtacata aaggttttgg cggtggaacg gggttatcag ctccggaata
      301 ttggcccttt cgacagcgcc gtttggagtt cggtctacgt ttcggaggag aaagtgctct
      361 tcgggttggc gagcggcaac ctgagcctgt ggaacgtgga gacagccaaa gagatacgac
      421 tggagcctca ggcccatagc gcggcgatcc gcgcgctgcg attctcgaaa aaaacgggac
      481 aggcgtactc cgcgtcgaac gacggtatga tcagaatctg ggataccgaa aacctgaatt
      541 tgatcgccac gtttttaatg gtggccaacg gcgaatggat atcctatatg ccgctgggca
      601 agtatgtatc cagccttaac gctttcgttc gccacgattt tttcgtgaag gacggagata
      661 aggaatacgc ctttgaccaa taccgcgact tccttcagaa agtggaacac ctcccgatcg
      721 gggacatctt cggcccggag gtgattctgg ccgatcacac cgtcacaccg cgacaaacga
      781 cactcaccct cgaaatcagc gatccgggga tcgtggaaga ggtgaacgaa tttggtatcc
      841 agtatccaat tcacgaaaaa cggacctcgt taccgatcac cttcgatatc tggaaacgtc
      901 cgtcgtctac gctcgaagta caggcggtcg actcttttgg aaacgtaagc caaacgacct
      961 ttggactgac ctttgcaggc tttcgcttct ttttgaaaga agagcatcca cccttgccga
     1021 aaaacgcgct ggtttccctc gacgctatcg tagataatct attcgtagtc ctctggcagg
     1081 gggaaaaagt aacggttccg aaagacaaag tgacccaaac gccgtacgcg cctgaaatat
     1141 ctatacaaat acagggagaa ccgcttgtga tgaaccaaac ggaatcccct cagctccgag
     1201 ttaaaatcac gatcgccgat atcttagggg taaaaagtgg caaagtcggg gcttggcagg
     1261 agatccgatt ccgagaaccg gtaaggcggt acgaactcga tcaaatcatc ccgctcgagt
     1321 tgggaaccaa tatcatcacg gctatagcca ccaacatcga agacatctcc caagagagta
     1381 gcctgaccgt tttccgaatc gaacgagaac cccctcagat cgtgatctcg cccgtgccat
     1441 ctcaaatata cgaagaccag ttcccgctcg gtattcgggt gtccgacaac cacatggtgg
     1501 ccttcgtgtc ggtaaacggc cgcgaagaac cggtgatgca aagccacaaa gacttaacgg
     1561 tcaccgtccc ggtcgcgata ggagagaacc gcgtggtggt ggaggccgtg gacgtcttca
     1621 aaaacgctgc gcgcaccctc gtccgattca acggagtccg aacgatgtac gcgaaaaatg
     1681 acggcatcaa ggtctccgac gcgcagggaa acctactcca catcttgatg aaaggggatc
     1741 ccgtcaccgt tttcagccat acccctccgc aaacttgggt cgaactcagc cggggtgttc
     1801 gcggggttgt ggcctcaagc gatcttcaac gggaggtggt ggatatttta cctccgggtt
     1861 tttgggacgt tcaagcgaag ttggacgggg actctgtatt agtcaccggc tttgtctacg
     1921 atgacaaaga agtagtctca cttttcgtcg caggtcaaca ggtacaagcg ctcacgcccg
     1981 tgaacatcac gatcccgaag gatggcgcga aaaaggtgta cgctttttcc atcaccctga
     2041 agccggcagc cgtcggcgcg ctcccggacg agataaaacc gatcgaattg gccgtgtatg
     2101 ataccgtcgg gttttttagc acactgatcg tcgttccgaa agtctatcgg tagaaaaagg
     2161 gaggagagcc ctatggagtg gatcgtgccc atcctttcaa cgctcgtcgg cttcgttatc
     2221 ggaagcttct tgaatgttgt gatctttcgt tttaaccaac cgtccctgac cgttttcaaa
     2281 ccgagcagat ccatctgtct gaactgcaaa aagccgattc cgtggtacga caatattcca
     2341 gtcctcagct tcgtcttttt acaaggtcgt tgtcgatact gccaaacgaa gttgtcgtgg
     2401 cgctatcctt ttatagaagc gctgactgcg gccggttttt tggttaacag cctgaccttc
     2461 gcctttaccc tccccttccc actcgcgctg gtaggggcgg atgtgttagc cggcgcgttg
     2521 atcgtcctgt tcttcaccga tttggacacg atgctgatct cggacgccaa caccgccttc
     2581 gtcatagccg gatgcgcgct cctctatctg gatcaagggt tacggatcga tcactctatc
     2641 ttcctcataa acctcatcac agcccttatc gcattctccc tgatctatat cctccgttta
     2701 ttgttcaaga tctttgggaa taaagaggcg atgggaatgg tcgacatcgt tttgatcagc
     2761 gcgctcgcgt tggccctcgg tccgatgaag gtcaacctgg cgattatcgt tgcctgtttc
     2821 accgggatac tcgccgcgct tttctttcgg aaaaagcttc gggacaaaat tcccttcggt
     2881 ccattcatcg cggtaggagg atacaccgct ttgattgcgg gagacgcgtt tttgaactta
     2941 atcggcttta tgccttattt ttagttctac aaccacacct gtgagcgccg cacctgtgag
     3001 cgccgcgcct gggagcgcct aactttagtt cggcatgttt cctggtttca tgtttgctag
     3061 gagcgccgca cctgggagcg ccggacttcg cttcggcagg gtttctggga gcgccgaact
     3121 ttagtacgtc caggtaaggc ggaaatgttc agcgggaaga aagcaagcct gccagtgaaa
     3181 aagtcaggac ctcgtagttt agccaacaag catcgaggaa atgaactgtc cggagcctgt
     3241 tgacaaaagc aatcgaacga ataaggatag tcgcccctct gaaggatagt cgcccctctg
     3301 gtgaaaaggg gccatacaaa cagatacagc ttcgtacgat aagtgtcata cggcaagtac
     3361 caaagcgtat cgcaccgtcc cggaaagaag aagcgcttac ggtgagatga ctacgaggat
     3421 catcgcggag caagcaaaaa acctcttcga tgaggtaagg aggaaaccta tgaaaaagat
     3481 gatgtgggcg acgctattcg cctgcttgta ttttgctacc ctattggccg gatcagaccc
     3541 gaaggctaca gcgaagacct atctagagtc ctttttcaag agagatttcg aaacgatgtg
     3601 gacccttcag gatgagacga tgcggcgagt attcgggaaa caagcgatgg aacaatcttc
     3661 caaaacgatc gaagtagcct tcggcgcctt tgaagccatc ctcagcatag aagaatcgga
     3721 agtccaaggc taccatgtgt ttctcttcta tacgaagatg caaaagtctt acgcgcagat
     3781 caccgtctcg gtagactcac agaaccttgt cgccggattt tatgcgaaac ctgcgccgtc
     3841 acctagcgct ttgacacccc cctacgcgga cacccagcgg ttcacagaag aggaggttgt
     3901 cttcggctcc gaacagtggc cggtttacgg cgcgctcacc ctcccgaaaa tagacgcgcc
     3961 ttatccgctc gtgatcctgg taggtggatc ggggccaaca gacagagatt caacgatagg
     4021 gcccaacaag ccttttcgag atctcgctta cgggctcagt tcagcaggcg tggcggtgtt
     4081 gcgatacgat aagcgacccg ccacacacgg agaacgtatg ctcctctcag agacttcaga
     4141 aagaggtgcc gtatgggatg agtatctaga agatctggaa gccgcaatcg cctatgcaag
     4201 cgaactagag agtatcggcc cgatctattt ggctgggcac agtttagggg gtcaggcggc
     4261 ggcctatgta gcaatagaat cagaatgcct ttcgggacta atactacttg cggctcccgc
     4321 acgccgcttc gcacagatca gtatcgacca gaaccggtac tttttctcgt cctccgggct
     4381 accgcaagag caactggatg cggtcgtctc gtttttccaa agcgttctca atcgggaatt
     4441 acctcccgaa atagagatac aaccgggatt gacggtcggt tatctttacg attgggatcg
     4501 ttaccagacg atggactccc tagaaaaggt gcgcttgccg gtcatcgcgc tacaaggtga
     4561 agaggatttc caagccacga tggatggaga ctttcttcct ttaaaagagg ccttcggaga
     4621 cagctctcga ttcactttca aatcctttcc ggggttgaac catctgttta tgagaaccga
     4681 gaccgacgtt ttccatacgg ttgacgaata ccaaaccgaa ggattcgtcg cctatgaggt
     4741 catcgacacg ctcctctcct ggatacgaca aacacgatcg gaatgatcac gtatacgaac
     4801 gatctctccg gtgttaaggc ggaaaccttc gttggatttt ttgaggggtg ggtacaaaaa
     4861 ccgacaccgg aacaacacct ggaaattctt cgccacagcg cgttctgttg gctcgctttc
     4921 gctgggaccc ggtgcgtcgg gttcatcaac gcgatatccg acgggttttt ttatgccgcg
     4981 attcccctgt tggaagtggt tccagaatac cgaggtaagg ggatcggtgc cgaactgata
     5041 cgaagaatgc gtcaaacgtt gaaagacttc tatgcggtag acctgatctg tgatgtggat
     5101 cctgactttg tcacgggaac accctcaaaa cggtataaac agcgaaaaag aaa
//
LOCUS       DAOPXN010000161         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16230_length_5144_cov_5.293378, whole genome shotgun sequence.
ACCESSION   DAOPXN010000161 DAOPXN010000000
VERSION     DAOPXN010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16230_length_5144_cov_5.293378"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(347..1645)
                     /locus_tag="PKM49_08300"
     CDS             complement(347..1645)
                     /locus_tag="PKM49_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNY82833.1"
                     /translation="MNTLVSEYQNKLTSPEKAVEIIPAKGSIVVGITIVEPPAILNAI
                     AERARSNGFEKLLVYTFNPQKYLTDSLLKPDLVDCVRTRAWFVSGPIRGLVKVGLIQF
                     VPSYFHEIPRLIRDMNVDTLITAVSPMDKSGYFSCCAASYVIDAARKAKNVIVEVNEN
                     IPRVFGDTLIHVSDVAAIVENHVPLLESKIPEKKPEDAIIGKEIAALIPDGAVLQLGI
                     GGLPNAICDYLTDHKDLGIHSELIGPGMIDLIKKGVITGRRKQLHPFKHVFGIPYGTK
                     ADFDFINDNISMESYSADYLMDPCVIGQNDHMISVNSILEVDLTGQCNAESISGSQFS
                     GTGGQVDFVRGAYRSKGGKSILAFYSTAKGGSISRIVPQLKHTSVTTPRVDVHYLATE
                     HGIVCLKGKSTEERAIAIAGLAHPNFREDLMRKAEEMGLL"
     gene            1995..2612
                     /locus_tag="PKM49_08305"
     CDS             1995..2612
                     /locus_tag="PKM49_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNY82834.1"
                     /translation="MKGMTFGATLKQLRKEHGIRQKDLANILKVDESTISSYERGIRY
                     PSRNVLKTLAVFFNVTTDSLLNVSASDRGMEGFLKGYNVPLLGRASAGAGAFAEENIE
                     EILVSDCDADFALRVHGDSMAPILPDRSVVLVKTVLPSKFKNSDIGVVIVHQDESLVK
                     YVHFHKEGVWLISENKAYTPVFFSQADWIESCELIGKVVECRIRF"
     regulatory      3105..3243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3233..4717
                     /locus_tag="PKM49_08310"
     CDS             3233..4717
                     /locus_tag="PKM49_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY82835.1"
                     /translation="MKRVISSLILFCFFVVGFSGSLTFFVNEKPFQTLTDGFDLSRIY
                     ELTFEGKDYDAVPLREFLPIMESVDQLVIANSKDGQTTVYEDVRRGLLPFSVALSRKE
                     PDQWLFFDGAHLFPFSSLSLFGDVAPKGALEVWVSWEGVPLLKTVIQQFSEHYGIPVK
                     VLEVPNTATKLLSMLKAGGKVPDLVMIQCEGFEELVLSKAFQQVDALYDGFESLYAPD
                     VFSLDNKRWAIPFYGDTQLLFYRKDRIPKLPEPLSLQELETLASELTTGDSLGLGWNL
                     FSVYWFAPFQIGFGKESILEPDGSVKIQDSATAQALAFLKTWVDRQIAIPLERDAMVS
                     MFTSGKLAMILSGSYSIPSFEELGIPFGITSYPYNAETGRWISPMLDFKGFGITRKTR
                     EPVLAMALLEYLTGPGAQTAFCIPVNKIPVNREVLGDQLETGGEVYQLYKHALDVGTY
                     VPAYNAYDLYKNTLWKLLRFVFLDQMSIPEVLEKGQQIIDANVP"
     gene            4839..>5144
                     /locus_tag="PKM49_08315"
     CDS             4839..>5144
                     /locus_tag="PKM49_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82836.1"
                     /translation="MLKKALFTVLLVALAVSSVFAWPLSLHGVREDSVSAGYFDSMKQ
                     HLTHYVEFAITEKNEVNRYHGMPLWLLIAMVDGKDSAHPYKFDLKRWEAGYEVKLIAS
                     "
BASE COUNT         1196 a         1311 c         1351 g         1286 t
ORIGIN      
        1 ttatcgcggg gtaaccagcg ggttgacttc ggaagcccac gaagtctcgt gatcccccta
       61 agaaactcgc gatccgcctt ttgtctttct cgaaagcgta tcgcgccctc gcgaaaagaa
      121 gaagcgctta cgccgcgatg ctgtgcctgg gaacgccaac ttttaagttg gtatgtctaa
      181 aaaagctcac acggaaagca ctgagggcac gaagcgatga gggagttacg agagtcgaca
      241 tcaggtttgt ggttttctct gtgctctgtg cgtactctgt gcgaaaccat tcttttcttt
      301 ttaaaattac ccaacttaag cgcggcgcct ccccaatctg agccgattat agcaacccca
      361 tctcttcggc tttacgcatc aaatcctctc gaaagttggg atgcgctaag ccggcgatag
      421 cgatcgcgcg ctcttccgtc gacttccctt taaggcagac gatgccgtgt tcggtggcca
      481 ggtaatgaac gtccactctg ggcgtcgtca ccgatgtgtg cttcaactgc gggacgattc
      541 tggaaatgct tccccctttt gccgttgaat agaacgccag aatcgatttt ccgcctttag
      601 accgataggc gccacggacg aaatcgactt gaccgcctgt gccgctgaat tgagagccgc
      661 tgatggactc cgcgttgcac tgaccggtca agtccacctc gaggattgaa ttcaccgaaa
      721 tcatatggtc gttctgaccg ataacgcacg ggtccatcag gtagtccgcg gaatagctct
      781 ccatactgat attatcgtta atgaaatcga aatcggcttt cgtcccgtaa gggataccaa
      841 atacgtgctt aaagggatgc agttgtttcc tccgccccgt aatcacccct ttcttgatta
      901 aatcgatcat ccctgggccg attaactcgg aatgaatacc caaatccttg tgatcggtga
      961 ggtagtcgca tatggcgttg ggcaagccgc cgattccaag ctggagaacg gcgccatcgg
     1021 gaatgagagc ggcgatctcc ttaccgatga ttgcgtcctc gggttttttc tcgggaattt
     1081 tgctttccaa caacggaacg tgattttcta cgatcgccgc gacatcggag acgtgaatga
     1141 gcgtatcccc gaaaacccgc gggatatttt cgtttacttc gacaatcacg tttttggctt
     1201 tcctggcggc atcgatcacg taactggcgg cgcagcagct gaaatagccg gatttatcca
     1261 taggcgagac agcggtgatc aacgtgtcca cattcatatc tcggattagc ctcgggatct
     1321 cgtggaaata ggacgggacg aattgaatca accccacctt caccaatcca cggatcgggc
     1381 cgctgacgaa ccaagccctc gtccgcacgc aatcgacgag atcgggtttt aacaaagagt
     1441 ccgttaggta cttttgtggg ttgaacgtat agaccaatag cttttcgaac ccgttggaac
     1501 gggcgcgctc ggcgatggcg ttcagaatcg ccggcggttc gacaatcgtg atgccgacga
     1561 caatcgatcc tttcgcgggg atgatttcta ccgctttctc tggagaagtc agtttgttct
     1621 ggtactccga aacaagggta ttcatatttc tctcactcct ttttttacga attgtagaca
     1681 cctttaggat aattgcgacc gtgtggatca gcggcgaagc gcccgtgata aacacgtggt
     1741 attttacacc ctagacgggg gcgatatcaa tacgggtttg ggcgagggtt atcggacgta
     1801 ttgtccagtg gaataagcga atggcccgct cacccttatc caatccggat attcgataac
     1861 cggcttccat tgagggttat aaacagagcg aaagaaatag ccggacatct tgtatgggtc
     1921 gctgatgttg tgttataatc aaaggggctc ggaacgcgga agagcggaaa aaccgaagcc
     1981 tttaggaggg actcatgaaa gggatgactt tcggagctac tctgaagcag ctacggaagg
     2041 aacacggcat tcgtcagaag gacctcgcga atatccttaa ggtggatgaa tcgaccatct
     2101 cgagttacga acgggggatt cggtatccat cccgcaacgt tctaaagacc ctcgcggttt
     2161 ttttcaacgt gacgacggat agcctgttga acgtttcggc ttctgatcgg ggtatggagg
     2221 ggtttctaaa agggtacaac gtcccgctct tggggcgcgc gtccgccgga gcgggcgcct
     2281 tcgcggaaga aaacatcgag gagatattgg tttccgactg cgatgcggac ttcgcgctca
     2341 gggtacacgg agacagcatg gcgccgatcc tgccagaccg gtccgtcgtc ctcgtgaaaa
     2401 ccgtcctccc cagcaaattc aaaaacagcg acatcggcgt cgtcatcgtc caccaagacg
     2461 agtcattggt caaatacgtg cacttccaca aagaaggagt ttggctgatc agcgagaata
     2521 aagcctacac ccctgtattc ttttctcaag ccgactggat cgaatcgtgt gaattgatcg
     2581 gcaaggtcgt ggaatgtcgg attcgtttct aattctggcg gtatcgacgg ccgattccga
     2641 ctaaccgttc cggtattcaa accgtaatgg aaatctaccg ttttaaaaaa ggttctttag
     2701 ccgttttagt tcgtcattct aaagacaaaa cccccttgcg ggggttttgt ctttaggggt
     2761 tcggggggtt ctcgtcggga gaaatctact atcccgttga ggatgttcga gactattata
     2821 ccccaactcg gacaaaatgt caagtctcga acttaaccct aaagtccaga atttatgcgt
     2881 ttcacgtaaa tcattttcag gagctggtta cccgtccttg ataggaataa tgatcgaaaa
     2941 cgagccgcaa gaaacccgta cattttgtac gtaactgtgc gcgaatagta ggggagcacg
     3001 gccttatggt ataccgaaaa atgcgtcgag gaagggcgcg ggtatagcgg tacccctttt
     3061 ccgaaagacc ccgtttgcgt agactgactt ttcgtggtac aatcgggtag tttcgcctcc
     3121 gagcttaagt gcctgccaat acgctggggc ttgagccggc gatgcatcct gatatctact
     3181 atagggtagg attggaaaca gtcctgcctc ccgagtttgg aaaggaggag aaatgaaacg
     3241 ggtaatctct agcttgatcc tgttttgttt tttcgttgtc ggtttttctg gcagtttgac
     3301 cttttttgtg aacgagaaac cctttcagac ccttaccgat ggtttcgatc tctcgcgtat
     3361 ctatgaactc acttttgaag gaaaggatta cgacgccgtt cctttgagag aattcttgcc
     3421 gattatggaa tctgtggatc aactcgtgat cgcgaactct aaggatggcc aaacgaccgt
     3481 atacgaagac gtacgtcgtg ggcttttacc tttcagtgtg gcgctttctc ggaaagagcc
     3541 cgatcagtgg ctgtttttcg acggcgccca ccttttcccg ttttcaagcc tgtcgctttt
     3601 cggcgacgtg gccccgaagg gggcgttgga ggtttgggtc agctgggaag gggtgccctt
     3661 gctcaaaacc gtcatccaac agttttccga acactacgga atccccgtga aggttttgga
     3721 agtaccgaat acggcgacca agctcctttc gatgctgaaa gccggtggaa aggttccgga
     3781 cctcgtgatg attcaatgcg aaggcttcga ggaactcgta ctgtccaaag ctttccaaca
     3841 ggtcgatgcc ctgtacgacg gcttcgagag cctctacgcg ccggacgtct tctctttgga
     3901 taataagagg tgggcgattc ccttctacgg ggatacccaa ctcctgtttt atcgaaagga
     3961 ccgaataccg aaactacctg agccactgtc cctgcaggag ctcgaaaccc tcgcttctga
     4021 gctcaccacg ggcgattctt tggggttagg atggaatctg ttttcggtct attggttcgc
     4081 cccgttccag atcggattcg gtaaggagag cattctcgaa ccggatggct ctgtgaagat
     4141 acaagactcg gcgaccgccc aagcgctggc ctttctcaaa acgtgggtgg accgacagat
     4201 cgccataccg ctggaacgag acgcgatggt ttcgatgttt acatccggaa agctcgcgat
     4261 gatcctaagc gggtcctatt cgatcccctc attcgaagag ttgggcatcc ctttcggaat
     4321 cacctcttat ccgtataacg ccgagaccgg tcggtggatc tccccgatgt tggatttcaa
     4381 aggattcggc atcacgcgta aaacccgcga accggtcctg gcgatggccc ttctggaata
     4441 tttgactggc ccaggggcac aaacggcgtt ttgtataccg gtgaacaaga taccggtcaa
     4501 ccgagaagtt ttgggggatc agctcgaaac cggaggcgag gtctatcaac tctacaaaca
     4561 cgccttggac gtagggacct acgtgcccgc ttacaacgcc tacgacctgt acaagaacac
     4621 gctctggaaa ctgttgcggt tcgtgtttct ggaccagatg tcgatccccg aagtgctgga
     4681 aaagggccaa cagatcatcg acgccaacgt accttgatcg cgtcgctttc gaccggtttt
     4741 ggaccagatc gctcactatg gttcggatgt atgacgacat ttcttgacct cgacaactca
     4801 caacaggggt aatctgcccc gcacaatata ggaggattat gttgaaaaaa gctttgttca
     4861 ctgttctttt ggttgcgctc gcggtatcgt ctgtgttcgc ctggccgctt tctttgcacg
     4921 gcgtgcgcga agactccgtt tccgcgggtt attttgattc catgaaacag cacctgactc
     4981 actacgtcga attcgcgatc accgagaaaa atgaggtaaa ccgctaccac gggatgcctc
     5041 tgtggctgct gatcgcaatg gtcgacggta aagactccgc tcacccctac aagtttgatc
     5101 tgaaacgctg ggaagccggt tacgaagtca agctcatcgc ttcg
//
LOCUS       DAOPXN010000162         5141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16242_length_5141_cov_5.479158, whole genome shotgun sequence.
ACCESSION   DAOPXN010000162 DAOPXN010000000
VERSION     DAOPXN010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16242_length_5141_cov_5.479158"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="PKM49_08320"
     CDS             <1..2153
                     /locus_tag="PKM49_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82837.1"
                     /translation="RQCVVDEILAGAGMPAFTMATQGQPGTYKYNLLADEFGFTPEGN
                     EALALKEIETAMQAAAALPANQGRLVKTGGKWMFDGKPVTLNFLIRVDDPSGRLRLGN
                     YFADQVEKSGITVNRLAWDRVKCATLAYYSDPKAAIEIGNIWHLYTEGWGAGETRAFW
                     EHIVAQMYAPWYGYMPGGAEPSNWNYENALIDDMTLKAYTGNFMTEEEYWNYALTGLR
                     EGLREAVRIYVANQTDFFVANKARFAQRFAYGLGDGLNEWSLITAKTVDKKLTVTQFS
                     ARGALFMASWNPVGADGFNDVYSNNIAGPLYDPGSFESPVSAISTPKRTIPRLDTLES
                     KVQAVGAGEDVKFVGLIDVPPSAIKYDAVSNSWKPVGDGVKAFSKCTYDFVYGKWHDG
                     TPQTLADLMYMQGFVEEWISKDGPEDKEFDDAYSSKLKPGNDTLKGWVLNADGSITTY
                     FDYNFPPSAPRVAANGAPGWTVSASGHAVGVAWSIYEALARMVADGGTQSGTTWSFSS
                     QAENEVDVLVPRCVADIRAKLQELYDKGHVPQYVVGYMTQKEAKDAYANAIKFIDKYG
                     HAYISNGPFWLEKYDPISNFMELTANRDPSYPYSSQYWTEVFAVPTISIDRITIPSLV
                     PAGKNLEVVVRVSKVVYPEVLAENAEEGRVTVRFLSSPEIVVQANLVRSGEFKAIIPG
                     TSLVNVPKGTYKVQIEAQLEGALEKSETKNVVIW"
     gene            2220..3269
                     /locus_tag="PKM49_08325"
     CDS             2220..3269
                     /locus_tag="PKM49_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82838.1"
                     /translation="MYWKYVIKRVINGIIIYIILIFIFSALFNMQMEQTVRAMIDEKV
                     KGEVMAQSTSGKWTPEQLMHWSKDRKTYYYELYNLNKPVFERIVSRTVDTLLFRYGKS
                     TIIKSSFGERDVVKIIFETVPRTLLLFGTAAIIDVLLGTLIGIKKAQKPGKPFDKGTS
                     IATMVVYGMPSWWLGMLMIMFFVYIVKVFPSGGMHSTPPPTGFMYHLDTLWHLTLPVL
                     TLVIIGFWGRAFLIRNIVLGVLQDDYIMSARARGIPERKVLFGHALRTAAPPIITMAL
                     LSLLVSVSGGILFESIFSWSGMGNLYWISVEQSDIPVLMGDLSFTTGLYICGLVILDL
                     IYGFLDPRIKVSGKA"
     gene            3266..4669
                     /locus_tag="PKM49_08330"
     CDS             3266..4669
                     /locus_tag="PKM49_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82839.1"
                     /translation="MKLKDLKVSISEFWQEFRKVKSGLVGLAIFGFFLALLIFEPVLA
                     PFSETNTRWRDISYWDDNPQSAYPIWVNFFSAKKYAESTLLENPEVKEEVFGTNRMVT
                     YTFEYDYQYDIAPADLILKVEGSGAAMMDLTFTRPDEESVLLASTNLNMADGGVNRIS
                     VDKGSKTEAYEFLGYFESRDNLRKFQRSSIKPAAILFAKAQENMLIEPEALKGKYTMQ
                     AALLLQRPSDIVRDVKLVIPGRVFGLLGTDAFKRDLFSGVLAGIKWALLIGLLTAVVS
                     VMVGVIYGVISAYLGGWKDSLMQRIFEIFNSIPLLPVLIVMSAIFKPSIWNLILIMCI
                     FYWTGSVKTVRSMGLQIKEETFIEATRALGASNFRIIFKHIIPLIIPYSFASMALFIP
                     GAIVYEASISLLGLGDATIVTWGQILRDAYTGGAVINGLWWWVIPPGLMIAIMGMSFA
                     FIGFALDKILHPKLRTR"
     gene            4675..>5141
                     /locus_tag="PKM49_08335"
     CDS             4675..>5141
                     /locus_tag="PKM49_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY82840.1"
                     /translation="MVAESARENKRVLEVKNLKLHYLTSQGAVKAVDDISFCLNRGET
                     LGIVGESGCGKTTTAVALMMMPSPPGKIVGGQILIEGKDIVPMTETQIRKQIRWEKIS
                     MVFQGAMNCLTPVYTIGKQMMETLREHREMAKEEAIARIEKYLAYVGLSRDVL"
BASE COUNT         1269 a         1319 c         1363 g         1190 t
ORIGIN      
        1 accggcagtg cgtggtagac gaaatcctcg ctggcgccgg tatgcccgcg tttacgatgg
       61 ccacgcaagg gcagccggga acttacaagt acaacctgtt ggccgacgaa ttcggattca
      121 cccccgaagg caacgaagcc cttgcgttga aagaaatcga gaccgcaatg caagccgctg
      181 ccgcccttcc tgccaaccaa ggcagattgg ttaaaaccgg cgggaaatgg atgttcgacg
      241 gcaagcccgt gacgctcaat ttcttgatcc gtgtcgacga tccgtctggc cgtttgcgct
      301 taggcaacta tttcgccgat caggtggaga aatccggcat caccgtgaac aggctggcgt
      361 gggaccgtgt caaatgcgcg accctcgcgt attattccga cccgaaagcg gctatagaaa
      421 tcgggaacat ctggcacctc tacaccgaag gatggggcgc cggcgagact cgagccttct
      481 gggaacacat cgttgcgcaa atgtacgctc cctggtacgg ctatatgccc ggtggcgcgg
      541 aaccgtccaa ctggaactat gaaaacgcct taatcgatga catgactctg aaagcctaca
      601 cggggaactt tatgaccgaa gaagaatact ggaactacgc gttaaccgga ctcagagaag
      661 ggctaagaga ggccgtccgc atttacgtgg cgaaccagac tgacttcttt gtagcgaaca
      721 aagccagatt cgcacagcgc ttcgcctacg gcctcggaga cggactcaac gaatggtccc
      781 tcatcaccgc gaagaccgtc gacaagaagc tgaccgtcac ccagttctcc gcaagaggcg
      841 cgctcttcat ggcctcttgg aaccccgtcg gcgccgacgg ttttaacgac gtctattcga
      901 acaacatcgc cggccctttg tatgatcccg gttcgtttga aagccccgtg tctgcgattt
      961 cgacgccgaa gagaactatc cccaggctag acactcttga atccaaagtg caggcagtgg
     1021 gcgcaggaga agacgtcaaa ttcgtcggct tgatcgacgt acctccctcg gcgatcaaat
     1081 acgatgccgt gagcaactct tggaaacccg ttggagatgg cgtgaaagcc ttctcgaaat
     1141 gtacctatga cttcgtttac gggaaatggc atgacggcac ccctcaaacg ctcgcggatc
     1201 ttatgtacat gcagggcttt gtggaagaat ggatcagcaa agacggaccc gaggacaagg
     1261 aatttgacga tgcctattct tccaaattga aacccggtaa tgataccctc aagggatggg
     1321 ttctgaacgc tgatggatcg atcaccacct atttcgatta taacttcccg ccgagcgcgc
     1381 cgagagttgc cgctaacgga gcccccggtt ggacggttag cgcctccggt catgcggttg
     1441 gagtcgcctg gtccatctac gaagcgctgg cgcgaatggt cgccgatggt ggcactcaat
     1501 ctgggaccac ttggagcttc tcctctcagg cggaaaacga agtcgacgtg ttggttccac
     1561 gctgcgtggc agacatcaga gccaagctgc aagaactgta tgacaaaggc catgtccccc
     1621 aatacgttgt gggctatatg actcagaagg aagcgaagga tgcctacgcg aacgccatca
     1681 aattcatcga caaatacggt cacgcctaca tctcgaacgg acccttctgg ttagagaagt
     1741 acgatccgat ttccaacttc atggaactga ccgcgaaccg cgatccttct tacccctaca
     1801 gctcccagta ctggaccgaa gtcttcgcgg tgcccacgat ttcgatcgac cgcatcacca
     1861 ttccgtctct ggttcctgcc ggaaagaacc tcgaggtcgt cgtgagagta tccaaggtcg
     1921 tttaccccga agtattggcc gaaaacgccg aagagggccg tgtcaccgtg agattccttt
     1981 cctctcctga aatcgtcgtt caagcgaacc tcgtccgatc cggagaattc aaagccatca
     2041 tccctggaac ctctctggtc aacgtgccga aggggactta taaagtacag atcgaagccc
     2101 agctcgaagg agctttagaa aaatccgaaa ccaaaaacgt cgtgatctgg tagtgagata
     2161 ttagaactca gtgcgggtag tgcctcctag gaggcactac ctgattttag ggggagacga
     2221 tgtactggaa atacgtcatc aaacgggtta tcaacgggat catcatatat atcatattga
     2281 tcttcatatt ttccgccttg tttaatatgc agatggaaca aaccgtccgg gctatgatcg
     2341 atgaaaaggt gaaaggggag gtgatggcgc aatcgacctc cgggaaatgg acacccgaac
     2401 aattgatgca ttggtcgaag gacaggaaaa cctattatta tgaactttat aacttgaaca
     2461 aacccgtttt cgaacgaatc gtcagtcgaa ccgtcgacac gctcctgttt cgctatggga
     2521 aatcgacgat catcaaatcc tccttcggtg agcgggacgt cgtaaaaatc atcttcgaaa
     2581 ccgtgccgag gacgttattg ctctttggaa cagccgctat catcgatgtg ttgttgggaa
     2641 cgctgatcgg aatcaaaaaa gcgcaaaaac caggaaaacc cttcgataag gggacttcca
     2701 tcgcaacgat ggtcgtttat ggaatgcctt cctggtggtt agggatgctc atgatcatgt
     2761 tctttgtata tatagtaaag gtctttcctt cgggtgggat gcattcgacc ccgcctccca
     2821 ccggcttcat gtatcatttg gacaccctct ggcatctcac gctaccagtc ttaacgttgg
     2881 tgatcatcgg tttttggggt cgcgccttcc ttatccgaaa catcgtgctc ggcgtattac
     2941 aggatgacta catcatgtcg gcccgggcga gggggatacc cgagcggaaa gtgctcttcg
     3001 ggcacgccct cagaaccgcg gcgcccccaa tcattacgat ggccctatta agtttgctgg
     3061 tttctgtcag cggaggaatt ctgttcgaaa gcatcttcag ctggtccggg atgggcaact
     3121 tgtattggat ttcggtcgaa cagagcgata tcccggtctt gatgggggac ctttctttca
     3181 ctaccggtct gtacatctgt gggttggtca ttttagacct tatctacgga ttcctagacc
     3241 ctcgtatcaa agtgagtggt aaggcatgaa gctcaaagac ctcaaggtat cgatatccga
     3301 gttttggcaa gaattcagaa aggtaaagtc cggtttagtc ggactcgcga tctttgggtt
     3361 tttcttagcc cttttaattt tcgagcccgt tttggcgccc ttctccgaaa cgaacacccg
     3421 ttggcgggac atctcttatt gggacgacaa cccgcaatcg gcctatccga tctgggtcaa
     3481 cttcttttcc gcgaagaagt acgctgagag tacgttattg gaaaaccccg aagtcaaaga
     3541 agaggttttc gggaccaacc gtatggttac ctataccttc gagtacgatt accaatacga
     3601 catcgcacca gccgatttga tcttgaaggt cgaggggtcc ggagcggcga tgatggacct
     3661 aaccttcacg cggcccgacg aagaaagcgt tctattggcc tctacaaact taaatatggc
     3721 tgatggaggg gtcaaccgca tctccgtcga caaaggctcc aaaacggaag cgtacgaatt
     3781 cctcggctat tttgaaagtc gggacaattt gcgtaaattt caacggtctt cgatcaaacc
     3841 ggccgccatt ctattcgcga aagcgcaaga gaatatgctg atcgaacccg aagccctcaa
     3901 aggcaagtac acgatgcagg ccgccctact gttgcagcgc ccctctgata tcgtcaggga
     3961 cgtcaaattg gtcatcccgg gtcgggtgtt cggcctcctg ggcaccgacg ccttcaaacg
     4021 cgatctgttc agcggtgttt tagccgggat aaaatgggcc ttgctcatag ggttgctcac
     4081 cgcggtcgtc tcggttatgg tcggtgtgat ttacggggtc atcagcgcat atttaggcgg
     4141 ttggaaagac tccttaatgc aaagaatctt cgagatcttc aactccattc ctctgttgcc
     4201 cgtattaatc gtgatgagcg caattttcaa gccgagcata tggaacctca tcttgattat
     4261 gtgtatcttc tattggacgg gttcggtcaa aaccgttcga tcgatggggc tgcagatcaa
     4321 agaagagacc tttatcgagg cgacgcgagc gttaggcgcc agcaatttcc gtattatttt
     4381 caaacacatc atccctttga tcattccata ctctttcgct tcaatggcct tgttcatccc
     4441 gggcgcgatc gtttacgaag cgtcgatctc cctgctgggt ttgggcgacg ccacgatcgt
     4501 gacgtggggg caaatactgc gtgacgccta cacggggggc gccgtgatca acggcctttg
     4561 gtggtgggtc ataccgccgg gattgatgat cgcgatcatg ggaatgagct tcgctttcat
     4621 cggtttcgcg ttagataaaa tactgcatcc gaaactacgg acgaggtaaa caaaatggta
     4681 gcagagagtg cgagagagaa caaacgtgta ttggaagtta aaaatttaaa acttcactat
     4741 ctgacgagcc aaggcgccgt caaggcggta gatgacattt ccttctgttt gaataggggc
     4801 gagacccttg ggattgtcgg agagtcggga tgcgggaaga cgacaacggc ggtggcgctg
     4861 atgatgatgc cctctcctcc cgggaagatc gtcggcgggc aaatcctcat cgagggaaaa
     4921 gacatcgtac cgatgactga aacgcagatc agaaagcaga tacgctggga aaagatatcg
     4981 atggtctttc agggtgcgat gaactgtctc acccccgtat acaccatcgg gaagcagatg
     5041 atggagacct tgagagaaca tcgggagatg gccaaagaag aggcgattgc cagaatagaa
     5101 aagtatctcg cttacgtggg cctttctcgg gacgttttaa a
//
LOCUS       DAOPXN010000163         5123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16318_length_5123_cov_5.962510, whole genome shotgun sequence.
ACCESSION   DAOPXN010000163 DAOPXN010000000
VERSION     DAOPXN010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16318_length_5123_cov_5.962510"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="PKM49_08340"
     CDS             <1..533
                     /locus_tag="PKM49_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82841.1"
                     /translation="NVLVTVGGADPLRLMEPLARALEPYPGQKTLIIGPAFGQPETER
                     LEALFFRGYELAIAPRSLKPHIRRAEVALTAAGSTIYEVLRLGRIPVLFELAENQRQI
                     ARVLEGAGIPNLGWHAQLTAERVFETVSQAVARKAQLQERCRPLFSLYDGQGVRRLVN
                     AIETLLSGNVTGTAEL"
     gene            896..1828
                     /locus_tag="PKM49_08345"
     CDS             896..1828
                     /locus_tag="PKM49_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HNY82842.1"
                     /translation="MGKEMLRPSKLTLGTVQLGMRYGIANNDGMPDESAAQEILEEAW
                     RRGITSFDKAAAYGESERRIGAFVKRLPELAAHLFIATKGSLEPSATLDDEAYKSALF
                     ERLEISRTALNLPEVPLYMAHRYEDYRHRLNAYTSFFSQAKDQGHIRLAGVSLYTPEE
                     AEEVLRLSRKAPLLDALQIPFNLFDRRFLENRLLDELKAAGFMIFIRSVYLQGLFFLS
                     PDTLPSALREAAPLLRRLQSLSSETGAGLREMALGYARERAAPDSILIGVETVTQLRE
                     NLDAYGKKLPSGLIEEIDHTFAKVPAPVLDPRKW"
     gene            2543..3442
                     /locus_tag="PKM49_08350"
     CDS             2543..3442
                     /locus_tag="PKM49_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82843.1"
                     /translation="MEKKYLNPLLDYTFKKIFGNEKHKEILIHFLNSLGIESQGHKIV
                     DLRILDHTNEKRFKLDKYSVMDIKATLNNGELVNIEVQILPYEHMLQRTIYYLTKMYS
                     DQLTQGDDYGKLCNTITVNVLGYNQFEHDRIHSVYHIRDAETCEKLGDFLEIHFIETR
                     KIEKSPQELGEDLKGWLLFLSNPENQRIKELGKEVGEVGQAINLLEELSRSREERAIA
                     EEREKAIRDERSFRKAELEKGLKLGREEGREEGREEGLKEGREAEKYQIAKKLIHKGM
                     DLPEVSEITGLSLKDLMNIDNLT"
     gene            complement(3726..>5123)
                     /locus_tag="PKM49_08355"
     CDS             complement(3726..>5123)
                     /locus_tag="PKM49_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82844.1"
                     /translation="FKTCHTEVWRSQVFLNVPAMRYVIILPNGKKRVKSMFEMPETIT
                     YALPERIGNTDLFVGRKKEFDYYLGDWYYYLVNNMARSQAIVARRRKGKTAFLERLFN
                     ILWSCSGNKVIPFFYVMQERQITLASFAKDFFATFVSHYLSFLTRNKDWVLSPLNYME
                     MKKYITQYPDLYKKSISIDEFEKTGNWDLMWSVARRTPFDIAVSTGVKVVQIIDEFQN
                     INAYIVNERGKTIDSMSGAYRDLAERKEAPLIVSGSEVHRLLEILCRLTAGFAENVLE
                     NLPEEEAKEAVWKYAQATDTRIDEHAVEKIWNLTQGDPLYLKALFLTRYNDTQDYTNE
                     DNIVATYTQEITRGEIYGIWGEYIAAFFLEANERDAKRIMLYLFQAGEERTRAQIIKD
                     LKLDMTDDELETKLQKLIKADLISQGETNFDYKIEKDKTYELVFRRLFQEEIDNCIPD
                     IRKELRQEMDGNAKL"
BASE COUNT         1317 a         1299 c         1294 g         1213 t
ORIGIN      
        1 gaaacgtctt ggtcaccgtc ggtggcgccg atcccctccg gttgatggag cccctggcga
       61 gagcgctgga accctatccc ggccaaaaga cgctcatcat cggtcccgcg ttcgggcagc
      121 ctgaaactga gcggttggag gcgctatttt tccgagggta cgaactcgcc atcgcgcctc
      181 ggtcgctcaa gccacacatc cgacgcgcgg aggtggcgct gaccgccgcc ggttccacca
      241 tctacgaggt gttgaggttg gggcgtatac ccgtcctctt cgagctggcg gaaaaccaaa
      301 ggcagatcgc gcgggtgtta gaaggagcgg gaataccgaa tctgggatgg cacgcgcagc
      361 tgactgccga acgggttttt gaaacggtct cacaagccgt cgcgcgaaaa gctcagttgc
      421 aagagagatg ccgaccccta ttcagcctct acgacggcca aggcgttcgc cgactcgtga
      481 acgccataga gacactcctc tctgggaacg tcactgggac cgccgaactt tagttcggca
      541 caggtttttt ggtttaccgc ggggtaacca gcgggctgac ttcggaaacc cacgaagtct
      601 cgtgattctc gtgaggggta atcgcggggt aagtggtttt tacgaccccg cgatcccgct
      661 tttgttctct ttacgccgcg atgatttcgg ggtatcgcgc tatggggtaa tcgcggggta
      721 accgaaggga ccccgcgatc ccccttttgt tttctttacg ccgcgatgcc acgcctgaga
      781 acaacctggg aacgccacac gactgggacc gctctgggaa cgccaacttt taagttggca
      841 tgtctttggt ttttcttgtc cgggtacact cagcgtcaaa aaggaggaaa gcaagatggg
      901 caaagaaatg ctccgaccct cgaaactcac cttaggcacc gtgcagttgg gtatgcgtta
      961 cggtatcgca aacaacgacg ggatgccgga tgaaagcgcg gcccaggaaa tactcgaaga
     1021 ggcttggcgg cgcggcatca cctcgttcga caaggcggcg gcctacggtg aaagtgagag
     1081 acgcattggc gccttcgtga aaagacttcc ggagctggcg gctcatctat tcatcgcgac
     1141 gaaaggatcg ctagagccgt ccgcgacgct cgacgacgaa gcctacaaaa gcgccttgtt
     1201 cgagcgctta gaaatttcta gaaccgcttt aaacctccct gaagtgccgc tctatatggc
     1261 gcaccgttac gaagactatc gccaccgcct caacgcctac acatcattct ttagccaagc
     1321 gaaggatcaa ggacacatcc gattggccgg cgtctctctg tacaccccgg aagaggccga
     1381 agaggtgctc aggctttcgc ggaaagcgcc ccttctcgat gctttacaga tcccgttcaa
     1441 cctcttcgat cgacggtttt tagaaaaccg actcctcgac gaactcaaag ctgcgggatt
     1501 tatgatattt atacgcagcg tctacctcca agggctattc tttttatcgc ccgacaccct
     1561 gcccagcgca ttacgagagg cagccccgct tctacgacga ttgcaatcct tatcctcaga
     1621 aaccggagct gggctacgcg aaatggcgct cggttacgct cgcgaacgcg ccgcccccga
     1681 cagcatcctc atcggcgtag aaaccgtcac gcaactacgc gaaaacctgg atgcgtacgg
     1741 aaagaagtta ccgagcggcc taatagaaga aatagaccac accttcgcaa aagtccccgc
     1801 acccgtcctc gacccccgca agtggtaact ctccccacag cgccttggga acgcccttct
     1861 gggagcgtct gggaacgcca cactttagtg cggcatgtct taaaaaactt gggttcacac
     1921 agagggcgca gagagcacag agaggacaca aagataaaat gcctggattt aaagcaaaag
     1981 atctagctcg cacagagggc gcagagaaca cagagagaac acaaagataa aatgcctgga
     2041 ttaaaaacat aagatcgagt tcgcacagtg ggcacaaggg gcacggagag gacacagagg
     2101 aaaaatgtct aaattaaaaa cacaagatcg agtttgcata gagagaacag agggcgcgga
     2161 ggaaagaagg ggtagattca aacgaaaacc tgtttggcat agaacgaacg gagagaaaag
     2221 gcttctaaag attcattgtt tatttctcta agctaaactt ttctcttctt tcttcgtgtt
     2281 ttctctgtgt tctccgtgtt ctctgtgcga aactttcttt tctcttcaaa aagacatgcc
     2341 aacttgaaag ttggcgttcc caagaagctt cctccgtgcc ctctgcgctc tctgtgcgaa
     2401 acccggttta ttttagacac accaactcaa aaggtgacgt tcccagaaaa acagtcgcaa
     2461 ataccaaaga acacacgaga cgagaactac gtccgaaaaa gtggtatgat taactcaaac
     2521 agtcactatg gaggtttccc caatggagaa aaagtacctg aacccactgc tcgactatac
     2581 cttcaagaag attttcggaa atgaaaagca caaagagatc ctgatccatt tcttaaactc
     2641 tttggggatt gaaagccaag ggcataagat cgtcgaccta cgcatactcg accatacgaa
     2701 cgaaaaacgg ttcaagctgg acaagtattc ggtaatggac atcaaggcga cgttaaacaa
     2761 cggggaactc gtcaacatcg aggtacagat actcccgtac gaacatatgc tccagaggac
     2821 gatatactat ttgacaaaaa tgtattcgga ccagctgacg caaggtgacg attacggcaa
     2881 gttatgcaac acgatcacag tgaatgtttt ggggtacaat caattcgagc acgacaggat
     2941 ccactctgtc tatcatatac gggacgcaga aacgtgtgaa aagctgggag actttctgga
     3001 gatacacttt atagagacgc gaaagattga gaagagcccc caagaattag gagaagacct
     3061 caagggttgg ctattgttct tgagtaaccc tgaaaatcaa aggataaagg agctcgggaa
     3121 ggaggtagga gaagtgggac aagcgatcaa cctgttagaa gaactgagtc ggagtcgaga
     3181 agaacgagcg atagcggaag agcgtgagaa agcgattcgc gatgaacgtt cctttcggaa
     3241 agcggaattg gaaaaaggat taaagttagg ccgagaggaa ggccgagagg aaggccgaga
     3301 ggaagggcta aaagaaggcc gagaagcaga gaaataccaa atcgctaaaa agctcataca
     3361 caaggggatg gatttgccag aggtctcaga gatcaccggg ctatctctca aggacttaat
     3421 gaatatcgat aacctcacgt aagtcaagga taagtcgctt tccggaaagg ccgcctgagc
     3481 tcgcaggcca cttcttgggt ctatcaggga tattctctgt gttctctccg tgctctcagt
     3541 gtgctctgtg cgaaacatgt ttcatttttt tagacctgcc aactgaaaag tcggcgttcc
     3601 catggatccc tgggcgtttc tctgtgttct ctctgtgctc tctgcgtact ctgtgcgaaa
     3661 ccttttttgg gttttgaaaa gacttacgaa ccaaaaaagt tggtgttccc agaaattatg
     3721 ctgaactaaa gtttggcgtt cccatccatc tcttggcgga gctctttccg gatatctggg
     3781 atgcagttat cgatctcttc ttgaaagagg cgtcggaaga cgagttcgta ggttttgtct
     3841 ttctcgatct tgtaatcgaa gttcgtttcc ccctgagaaa tcaggtcagc tttaatcaac
     3901 ttttgcagct tcgtttccag ttcgtcgtcg gtcatatcaa gcttgaggtc tttgattatt
     3961 tgcgctcgag tgcgttcttc gccagcctga aacaggtaca gcataatccg tttcgcatcc
     4021 ctttcgttcg cttccaagaa aaatgccgca atgtattcgc cccagatgcc gtatatctcc
     4081 cctcgcgtga tctcttgtgt gtacgtcgcg acgatgttgt cttcgtttgt gtagtcttgt
     4141 gtatcgttat agcgggtcaa gaataacgcc ttgagataca acggatcccc ttgggtcagg
     4201 ttccatatct tctcaacagc atgttcatcg atgcgtgtgt ctgttgcctg cgcgtacttc
     4261 cacacggcct ctttcgcctc ttcttcaggg agattttcca ggacgttctc tgcgaaccca
     4321 gccgtgagcc tacataagat ctccaacaac ctgtggactt cactaccaga aacgatgagt
     4381 ggcgcctctt tcctctcggc taggtcccgg taagccccgc tcatcgagtc gatagtcttt
     4441 cctcgctcat ttacaatgta cgcgttgatg ttttgaaatt cgtctatgat ttggacgact
     4501 ttaacccctg tcgaaacagc gatatcaaat ggagtccgtc tcgcgaccga ccacatcaaa
     4561 tcccagttgc ccgttttttc aaattcgtca atcgatatgc tttttttata gagatcaggg
     4621 tattgggtaa tgtatttttt catttccatg taattgagag gggataagac ccagtctttg
     4681 ttccgagtga ggaatgaaag gtagtggctt acgaacgtcg cgaagaaatc tttcgcgaag
     4741 ctggcaagcg tgatctggcg ctcttgcatc acatagaaaa aaggaatcac cttgtttccc
     4801 gaacagctcc acaggatatt gaacagtcgc tccaggaagg ccgtttttcc tttcctccgt
     4861 ctggcgacaa tcgcttggct tcgagccata ttattgacga ggtaatagta ccaatctccc
     4921 agatagtagt cgaactcctt ctttctcccg acgaagaggt cagtgttccc gattcgctcc
     4981 ggcaaggcgt aggttatcgt ttccggcatc tcgaacatcg atttcacccg ctttttcccg
     5041 tttggtaaga ttataacata gcgcattgcc gggacgttta ggaacacctg ggaacgccac
     5101 acttcagtgt ggcatgtctt aaa
//
LOCUS       DAOPXN010000164         5105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16399_length_5105_cov_5.208713, whole genome shotgun sequence.
ACCESSION   DAOPXN010000164 DAOPXN010000000
VERSION     DAOPXN010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16399_length_5105_cov_5.208713"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKM49_08360"
     CDS             <1..215
                     /locus_tag="PKM49_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82845.1"
                     /translation="AVGMESREEYADIELIDLTHDILLYIEDKTGKTDGVISNLPEDD
                     KLCEVFPLLGAQMIFSQLEMELKLRV"
     gene            335..955
                     /locus_tag="PKM49_08365"
     CDS             335..955
                     /locus_tag="PKM49_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HNY82846.1"
                     /translation="MSAAYGPQNWWPGDSVYEIMVGAILTQNTQWTNVEKAIRSMKEM
                     GILEPRKIASLPLDTVKAAIKDAGCYNQKATRLKNLTAFLIEKNLDFFRLRKAETAVL
                     RNELLGVNGIGKETADSILLYALGHPVFVVDAYTKRLFSRLRYRWMAKGSYDEIADFF
                     HHQFPRDAVRYNEFHALIVFHSKYYCRVRPGCASCVLKDLCYYDQS"
     gene            983..1519
                     /locus_tag="PKM49_08370"
     CDS             983..1519
                     /locus_tag="PKM49_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNY82847.1"
                     /translation="MYFDVISIGGPILVVILVIAGIGLFFFVERFIVLAAIKSAIRKG
                     DDEQTFLVKVDRRLSAQKGARADEIHEIISLMVERLTRNFRFISTAATVAPLLGLLGT
                     VTGMVKIFNSLDKTENLKYAGQLAMGIGEALYTTIAGLIVAITLTVLLNIIKEMITSI
                     ENELTDIYLKAPVTKGLL"
     gene            1531..1935
                     /locus_tag="PKM49_08375"
     CDS             1531..1935
                     /locus_tag="PKM49_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNY82848.1"
                     /translation="MSRRRTEEEININITPLIDIMFILLLFWVLTTTFDIGTVRSIDL
                     TLPEASTATDKGFNEFFNVSINAKDEIYIGKKAVTLSQISAEIERAFKSNPSLKAAIL
                     ADQKASHGTVVAVLDAFRTHRIYDVNIQATVK"
     gene            1953..2735
                     /locus_tag="PKM49_08380"
     CDS             1953..2735
                     /locus_tag="PKM49_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82849.1"
                     /translation="MPLNPIHIIAAMVAVMLEVLILALFIYIIRSAPYEIEREPPMRI
                     NLTAATVQKEEIPEKPQPPIPSVTPKQPEPEKPIETTPEPVKEVEVDPLLEQLLAFEP
                     KALSTPQKATTYLPQTRDTPAPMAPQAGLFDVQDIPLPPSQSLAPPKRYQSVYRENEY
                     RAVRFEELKEYAFPNYEEVAAALKKDYNDLLKKQGSRASLLEGNVVVNMRFEKTGFPT
                     VDTVSSPSPELANIVLRNMKLLRSGQNTEEMTFQVSLSFQIK"
     gene            2762..3445
                     /locus_tag="PKM49_08385"
     CDS             2762..3445
                     /locus_tag="PKM49_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HNY82850.1"
                     /translation="MFNIAVIVNTATVFFGALLGLKVGHKIPEAFRDISFTIIGTVTL
                     LIGVQMGLETKNMMVVLLSLVIGGVIGQALGIEEGIGRFVDRFGKKGGENQAVKGFIT
                     STLLFVSGPMTILGSFQAGVSGDYSIIFLKSALDGISALLLGSLYGVGVLFTAGSVFL
                     IQGFLVSFSSVLTFLTQPEYLKDFTGVGGMMLVIIGLRMMGIKETKVGNLLPALLVVL
                     IANAILLRF"
     gene            complement(3467..3592)
                     /locus_tag="PKM49_08390"
     CDS             complement(3467..3592)
                     /locus_tag="PKM49_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82851.1"
                     /translation="MKRLLRKATPSILYQERAYRVERVFLPYVDEQNWRNGTFIR"
     gene            3990..4853
                     /locus_tag="PKM49_08395"
     CDS             3990..4853
                     /locus_tag="PKM49_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNY82852.1"
                     /translation="MDAWLNGEFVKKDAPAVCFEDRGNTFGDGLFEVCRVLKRRPLFF
                     EEHLKRMEVSAAYLKLRFPYSLEEIYRATRELIERNGIETGEAYYQLTRGKDANREHR
                     IRPANLSPTFFILALPLREVSLKNWETGAQVYLYPDRRHLLCEHKTLNLLANVLAKNS
                     ANDRGGYEAMMYRRDARGIYVTEGGSSSFFCVHQESLLTPEIDNILPGVTRKKVLEYA
                     ASIGIRTIERRVYLKEFLTAEEVFLASTVSKVMPVRAIENQRFPTIGKITAIMMKEIE
                     RRIEENIRAQL"
     gene            complement(4858..>5105)
                     /locus_tag="PKM49_08400"
     CDS             complement(4858..>5105)
                     /locus_tag="PKM49_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNY82853.1"
                     /translation="AQGMKNALRKLIEEFQKDFASEKIEKAALYHSGDRQGVQDLLDV
                     VSDVLVRAKVPYIEGHISPSLVVHTGEHLVGLAYLMV"
BASE COUNT         1238 a         1358 c         1394 g         1115 t
ORIGIN      
        1 cggcggtggg aatggagagc cgcgaagaat acgccgacat cgagctgatc gatctgacgc
       61 atgacatcct gctgtatata gaggacaaaa cggggaaaac ggacggcgta atctcgaact
      121 tgccggaaga cgacaagctc tgcgaggtct ttcccctgct gggcgctcag atgatcttta
      181 gccaactgga aatggaattg aaactgcgcg tatgagccgc caaactccgg aagcaaaggc
      241 ggccccgacg acgcggcaac aacccttaga ggcagaacaa gaagccttcg gaagccttcc
      301 gcctcggaat ttttgcgcag agctgtacga ccggatgtcc gccgcttacg gtccccagaa
      361 ttggtggccc ggggacagcg tctatgaaat tatggtcggg gccattctga cccaaaacac
      421 ccagtggacg aacgtggaga aagcgatacg gagtatgaaa gagatgggca tcctcgagcc
      481 gcgcaagatc gcctcccttc cactggatac cgtgaaagcg gcgatcaaag acgctgggtg
      541 ttacaatcag aaggccacac ggttgaagaa tctcaccgcc tttctgatag aaaagaacct
      601 cgattttttt cgtcttagaa aggcggagac ggcggtttta cgaaacgagt tgttaggggt
      661 taacggcatc ggtaaagaga cggcggacag catcttgttg tacgccctcg gccacccggt
      721 gttcgtggtg gacgcctaca ccaaacgcct tttttcccga ttgcggtacc gatggatggc
      781 gaaggggtct tatgacgaga tcgcggactt cttccatcac caattcccga gagacgccgt
      841 gcgatacaac gagtttcacg cgctcatcgt gttccactcc aaatactact gtcgggttcg
      901 tccaggatgc gcgtcctgtg tgctcaaaga cctctgctat tacgaccaat cgtaggtttt
      961 ttaatgagaa gggggtatca tcttgtattt cgatgtgatt tctatcgggg ggccgattct
     1021 ggtggtgatt ctggtgatcg ccgggatcgg gttgtttttt ttcgtcgagc gattcatcgt
     1081 cctcgccgcg atcaagtcgg cgattcgcaa aggggacgac gaacagacct tcctggtcaa
     1141 ggtggatcgg agattgagcg cacagaaagg cgcgcgggcc gacgaaatcc acgagatcat
     1201 cagcttgatg gtcgaacgcc taacgcgcaa cttccgcttc atctccaccg cggcgacggt
     1261 ggcacccttg ttggggctgc tcggaacggt gacggggatg gtgaagattt tcaactcgct
     1321 ggataaaacc gagaacctca aatacgcggg gcaactggcg atggggatag gggaggcgct
     1381 ctacaccacc atcgcggggt tgattgtcgc catcacgctg accgtgttgc tgaacatcat
     1441 caaagaaatg atcacctcga tcgaaaacga gctgacagac atctacctga aagccccggt
     1501 cacaaaaggg ctcttatagc ggagtcttgt atgagcagac gtcggacgga agaagagatc
     1561 aacatcaaca tcacgccgtt gatcgacatt atgttcatac tgctgttgtt ctgggtcttg
     1621 accaccacat tcgacatcgg gaccgtccgc agcatcgact tgacgcttcc tgaagcctcc
     1681 accgccaccg ataagggatt caacgaattc ttcaacgtgt cgatcaacgc gaaagacgaa
     1741 atatacatag ggaaaaaggc ggtcacactg tcccaaatat cagccgaaat cgaacgggcc
     1801 ttcaaatcca acccctcgct gaaagcggct attctcgccg atcaaaaagc gagtcacgga
     1861 acggtcgtcg ccgtgctgga cgcctttcgc actcaccgaa tctacgacgt caatatccag
     1921 gcgaccgtca aatagtagaa atggaacagc gaatgccctt aaatccgatc cacatcatcg
     1981 cggcgatggt ggccgtgatg ctggaagtcc tgattctggc cctcttcatc tacatcatac
     2041 gctccgcacc ttacgagatc gaacgtgagc cgccgatgag aatcaacctg acagcggcca
     2101 ccgtgcaaaa agaagagata cccgaaaagc cgcaaccgcc gattccatcg gtgacaccca
     2161 agcagccgga acccgagaag ccgatcgaaa cgacgcccga accggtcaaa gaggtggagg
     2221 ttgacccgct gctcgaacag cttttagcct tcgagccaaa ggcgctgtcg accccgcaaa
     2281 aggccaccac ctatctgccg cagacgcggg acacgccggc ccctatggcg ccgcaagccg
     2341 ggctttttga cgtccaagac atcccgctcc cgccctccca gagcctcgct ccacccaagc
     2401 gataccaatc ggtctaccga gagaacgagt atcgcgccgt tcggtttgaa gaactgaaag
     2461 aatacgcatt cccgaactac gaagaggtgg ccgctgcctt gaaaaaggat tacaacgatt
     2521 tgctgaagaa gcaggggagt cgggccagcc tgttggaggg gaacgtcgtc gtgaacatgc
     2581 ggttcgaaaa aaccgggttc cccacggtcg acacggtttc ctccccgtct cccgagctcg
     2641 cgaacatcgt gcttaggaat atgaaactgc tgagaagcgg gcaaaatacg gaagagatga
     2701 cctttcaggt cagtttgagc tttcagataa aatagaaccc cacagaaagg aaggaatcgg
     2761 gatgttcaac atcgcagtga tcgtcaacac cgccaccgtc ttcttcgggg cgcttctggg
     2821 cttgaaggtg ggccacaaga ttccggaagc ctttcgcgac atctccttca cgatcatcgg
     2881 gaccgtgacc ttgctcatcg gcgtccaaat ggggctggaa acgaagaaca tgatggtcgt
     2941 tctactctct ttagtcatcg gaggcgtgat cggtcaggca ttgggaatcg aggaaggaat
     3001 cggcagattc gtcgaccgct ttgggaaaaa aggcggagag aaccaggcgg tgaaagggtt
     3061 catcacgtcc accctcctct tcgtctcggg acctatgacg atcttgggga gtttccaagc
     3121 gggggtatcc ggcgactact ccatcatttt cctaaaatcc gcgctcgacg gcatctcggc
     3181 gctcctactc ggctcgttgt acggcgttgg cgtcctcttc accgccggaa gcgttttcct
     3241 catacagggt ttcctcgttt cgttctcctc ggtcctgacc tttttaactc aacccgaata
     3301 cctcaaagac ttcacggggg taggtggaat gatgttggtg atcatcgggt tgcggatgat
     3361 gggaatcaaa gagacgaagg tgggcaacct cttgccggcc ctgttggtcg tcctgatcgc
     3421 caacgccatc ctgctgaggt tctgagggtg cgaaaaataa gctcagttag cgtatgaacg
     3481 tgccgtttcg ccaattctgc tcatcgacat agggaagaaa gacccgctcg acccggtagg
     3541 ctctctcctg ataaaggatc gacggcgtcg cttttcgcaa tagacgtttc atctgtgtcc
     3601 ctcctttcct cgcttcaaat tcagtccgac cacgcggccc ttgtcctctc gttcttttgg
     3661 actcttatgt cctatcattt ttcatacctc atcatcatat tattatgggt attatttgat
     3721 tttcgagatt ataccatacg aaaagaataa tatcaagtct tgattcataa ttatttattt
     3781 gtaacctgat ttttcttatt actctaaggc atagcacata tttctgacag aatactaagg
     3841 agttttcatc aatttggcgt gaatcttgac ccgaccgagg gcacccgagc gggggagatc
     3901 gtcagggctc acccgacgac cggtttactt ttcggtgaga aacggttata ctaaggggtg
     3961 gcagacattt ctacgttagg ggtgaaggta tggatgcttg gctgaacgga gagtttgtga
     4021 agaaagacgc gcccgcggtc tgcttcgaag atcggggaaa caccttcggg gacggactgt
     4081 ttgaggtatg ccgggtgtta aaacgaagac cattgttttt tgaggaacac ctgaaacgca
     4141 tggaggtcag cgcagcgtat ttgaagctca ggttcccgta ttctttggaa gagatctata
     4201 gggccactcg ggagctgatc gagcgcaacg gcatagagac cggcgaggcg tactaccagt
     4261 tgacgcgcgg gaaagacgcg aaccgagagc atcgtattcg gcccgcgaat ctctcgccga
     4321 ccttttttat cctcgctttg cccttacgcg aggtctctct caagaattgg gagacgggag
     4381 cccaagtcta tctatacccg gacaggcggc atctcctctg cgagcacaag acgctgaacc
     4441 ttttggctaa cgtgctcgcc aaaaactctg ccaacgaccg cggtggttac gaagcgatga
     4501 tgtatcgaag agacgcgcga gggatttacg tgacggaggg gggcagctcg tcttttttct
     4561 gcgttcacca ggagagcctc ctcaccccgg agatcgacaa catcctaccc ggggtgaccc
     4621 ggaaaaaagt gttggagtac gccgcttcta tcggtattcg gacgattgaa aggcgggtct
     4681 acctcaaaga atttttgacc gccgaagagg tcttcctcgc cagcaccgtt tcgaaggtga
     4741 tgccggtgag ggccatcgaa aaccaacgct ttccgacgat cggaaagatc acggcgataa
     4801 tgatgaaaga gatcgagcgg cgtatcgaag agaacatccg cgcccaactg taaggggcta
     4861 gaccatcaaa taggccaaac cgaccaagtg ctccccggtg tggacgacca aagaggggga
     4921 gatgtgtccc tcgatgtacg ggaccttcgc gcgcacgagc acgtcggaca cgacgtctaa
     4981 aagatcctgg acaccttgcc gatcgcccga atggtagagc gcggcctttt cgatcttttc
     5041 ggaggcgaaa tccttctgga actcctcgat gagttttcgg agggcgttct tcatcccctg
     5101 ggctt
//
LOCUS       DAOPXN010000165         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16407_length_5104_cov_5.954248, whole genome shotgun sequence.
ACCESSION   DAOPXN010000165 DAOPXN010000000
VERSION     DAOPXN010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16407_length_5104_cov_5.954248"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PKM49_08405"
     CDS             complement(<1..799)
                     /locus_tag="PKM49_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82854.1"
                     /translation="MFKDIGKLFLGNKKALFGLLLLLFFALVGLLAPVIAPYDPKSNR
                     VETITVLSEERDRSRTISREDLDPSTEKKVYSQSVTTTYERITKKLPLKGKPSSAHLL
                     GTTHSGQDIFSQLIWGTRVSLTVGLVTGLFTTVLSLCLALIAGYFGGLVDDVISLFTN
                     VFLVIPSLPLMIVIAAYVTVRGVIPIILILGLTSWPWPTRVLRSQVVSLKNRDFVRVS
                     KSLGEKPSYVVFREILPNMISLVMASFFSSTMAAIIGEATLEFIGLGN"
     gene            complement(799..1779)
                     /locus_tag="PKM49_08410"
     CDS             complement(799..1779)
                     /locus_tag="PKM49_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY82855.1"
                     /translation="MKYFFRKIGLLLFALFVAMTLNFFIPRMMPGDPARELIDRMQGI
                     PPEAMESIRIAFGLDTKDTLIVQYGKYLLNVLKGELGISLSRFPTPVRDVLKIAIPWT
                     LGLMGFATILSFFIGTILGIGAAWSRNKRWATLTVGTFIFIRSFPYFWLGLLFIYFFG
                     FRLQVFPIGNAYSFYVQKGGWDYFLSVLYHGFLPALTITVSSLGGWILTMRNNMINVL
                     AEDYITVALAKGLPLRRIKNLYAAKNAMLPSITSFAMSLGFVVGGGLLTEMVFAYPGV
                     GLMLWQAVQAKDYAMMQAIFLFISVAVLIANFLADLAILLLDPRVRDSGR"
     gene            complement(1870..3522)
                     /locus_tag="PKM49_08415"
     CDS             complement(1870..3522)
                     /locus_tag="PKM49_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82856.1"
                     /translation="MKKVGVVVLLLLLVLSSLFGAEAIKRGGMLRVTPMKQGVLIQNF
                     NPFSPNSLEVAVGCFYETLVFYNQGTGKFHPWLATAYEWSNDLKELTFTLRDGVVWND
                     GTPFTIQDVLFTMELGKKFPGLNIAPIWKQGLVDVVARTSNSVTFVFDRVNTTLIYRV
                     ADIFIVPRHIWKDVEDPLTWTGNLQPVGTGPFVYEAGSFTEQSFKVIKNPRYWQIAPD
                     GKPLPYIDGIVYISTTNEQVGLRLARGEYDWAGYFVANIDEVYVQADPEHHKYWLPEG
                     NLVYLNLNNLREPFNNYNVRYALNFALDNKEITRIMASGAVPSSPGWVKTPFLYLAKE
                     ALDKAGIEYDPDYTKELLEKEGYRLNAKGIYEKAGKALSFNLYVPTGWTDWIMGCEEI
                     ARQLKEVGIEAIVTQAAWPSPYVDDYLYYGNYEMCLGISVTGAGPYFQLEYIAHPRAW
                     APLGERSANYYGMRYKSEEIGRLLDSYTQEPLESKRDEIMKKALSLFLKDSPSVPLFF
                     NPTWYEYNTKLFVGWPSEEDPYCFPSPNGMHKLPLLLKVHLR"
     gene            complement(4528..>5104)
                     /locus_tag="PKM49_08420"
     CDS             complement(4528..>5104)
                     /locus_tag="PKM49_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82857.1"
                     /translation="LENLPEEEAKEAIWKYAEIYRTKIDNAGVDKLWNLTRGDPLYIY
                     ALFSSYHNAKKDYDREDNVIEVYTQEIKSGLIFKTWGEYLAGVFLEANERDAKRIMLY
                     LFQAGKERTRARIIKDLKLDITDSELKTKLQKLIKADLISYGSTASRYVIAKDKTYEL
                     LFRRLFQKEIDNSVPDIRKELSREMGGNAAL"
BASE COUNT         1247 a         1349 c         1294 g         1214 t
ORIGIN      
        1 cgttacctaa cccgataaac tcgagggtcg cctctccgat gatcgccgcc atcgtcgagg
       61 agaagaacga ggccatcact agagagatca tgtttggcaa aatctctcta aacacgacat
      121 aggagggttt ctctcctaag gattttgaga ccctgacgaa atcgcgattt ttcaaagaga
      181 cgacttgcga acgcaggacg cgggtcggcc aaggccagct tgtcaaaccc aagatcagga
      241 taatcgggat cacgccgcgg acggtcacgt aagccgcgat gacaatcatc agcgggaggg
      301 aggggatcac caaaaagacg ttcgtaaaga gcgagatgac atcgtccacc agaccgccga
      361 aatagcccgc gatcagcgcc aaacagaggg acaagaccgt ggtgaacaac ccggtgacca
      421 atcccaccgt caagctgaca cgcgttcccc agatcaattg tgagaagatg tcttgccccg
      481 aatgggtggt acctaaaagg tgcgcggacg aaggttttcc tttcaaaggc agttttttcg
      541 tgatccgctc ataggtcgtg gtgacactct gcgaatagac ctttttttcg gtggacggat
      601 ccaaatcctc tcggctgatc gttcgagagc ggtcgcgctc ctcgctcagc accgtgatcg
      661 tttccacccg attactcttt gggtcgtacg gggcgataac cggggcgagg aggcctacca
      721 aggcaaaaaa taggagtaac agcaggccaa ataaggcttt cttgttccct aaaaacagtt
      781 ttccgatgtc tttgaacatc agcgaccact atcccttacg cgaggatcca gtagaaggat
      841 cgcaaggtcc gcaaggaagt tcgcgatcaa aacggcaacc gatatgaata aaaagatcgc
      901 ctgcatcatc gcgtagtcct tcgcttgcac ggcctgccac agcatcaggc ccacaccggg
      961 ataggcgaag accatctcgg tcagaagacc gccgcctacg acaaatccga gcgacatcgc
     1021 aaaactggta atagagggta gcatcgcgtt cttcgccgca tagaggttct tgatccggcg
     1081 cagcggcagc cccttcgcca aggcgacagt gatatagtct tcggccaaaa cgttgatcat
     1141 attgtttctc atcgttaaga tccatcctcc gagagagctc accgtgatag tgagggcggg
     1201 caagaagccg tgatagagaa ccgagagaaa atagtcccat cccccctttt gaacgtagaa
     1261 ggaataggcg tttccgatcg ggaaaacctg caagcgaaag ccgaaaaaat agatgaacag
     1321 cagccccaac cagaagtagg ggaaggaccg gatgaaaatg aaggtcccca ccgtcagcgt
     1381 tgcccaacgc ttgtttcggg accaggcggc cccaattccg aggatcgtac cgatgaaaaa
     1441 gctgaggatc gttgcgaaac ccattaagcc tagggtccac gggatcgcga tcttcagcac
     1501 gtccctaacg ggcgtaggaa agcgagataa agaaatgccg agctctccct tcaacacgtt
     1561 cagcaggtac ttgccgtact ggacgatcag tgtgtctttg gtatctagcc cgaaggcgat
     1621 ccggatcgac tccatcgctt cgggcggaat gccctgcatc cggtcgatca actccctggc
     1681 ggggtcaccc ggcatcatac ggggtataaa gaagttcaac gtcatcgcca caaagagggc
     1741 gaacaacaac aagcctatct tgcgaaagaa gtatttcacg atttccccct atccagcttt
     1801 cgacgcacag ttttttgaag atagagacgc ccagaagggc gtctctatgc gctctctttt
     1861 gaatgcgcct tagcgcagat gaaccttcag caataggggc aacttatgca tcccgttcgg
     1921 gctggggaag cagtagggat cttcttcgct cggccagccg acgaagagct tggtgttgta
     1981 ctcgtaccac gtcgggttga agaacaacgg cacggaaggc gagtctttga ggaagaggga
     2041 gagagccttt ttcataatct cgtcacgctt cgattcaagc ggttcttgcg tgtaactgtc
     2101 gagtagtctt ccgatctctt cgctcttgta gcgcattccg tagtaattgg ccgaacgctc
     2161 gcccaaaggc gcccaagctc tcgggtgcgc gatgtattcg agttggaaat atgggcccgc
     2221 gccggtgacc gagataccca agcacatctc gtagtttccg taatacaggt agtcatccac
     2281 gtacggagaa ggccacgcgg cctgtgtgac aatggcctcg atcccgactt ccttcagctg
     2341 tctggcgatc tcctcgcagc ccataatcca gtccgtccaa ccggtgggaa cgtagaggtt
     2401 gaaggaaagc gccttgcctg ccttttcata gatccctttg gcgttgagtc gatacccttc
     2461 cttttccaac agctctttgg tgtaatccgg gtcatactcg ataccagcct tatctagagc
     2521 ttctttcgcc aggtacagga agggcgtttt cacccagccc ggagaagagg gcacggcgcc
     2581 gctggccata atgcgcgtga tctccttgtt gtccaacgcg aagttcaggg cgtatctcac
     2641 gttatagttg ttgaaaggct cgcggaggtt gttcaagttc aaataaacca ggtttccttc
     2701 aggcagccag tacttgtggt gctccggatc ggcttgaacg tacacttcat cgatattggc
     2761 cacgaaatag cccgcccaat cgtattctcc acgggccaac cgcagaccga cttgttcgtt
     2821 ggtggtcgaa atgtacacga tgccgtcgat ataaggcaac ggcttgccat cgggcgctat
     2881 ttgccagtac cttggattct tgatgacctt aaaggattgt tccgtaaagg accccgcttc
     2941 gtacacgaag ggaccggtcc cgaccggttg caggtttccg gtccaggtta acggatcttc
     3001 tacgtctttc cagatatgtc gcggcacgat aaaaatgtcg gccacacggt agataagagt
     3061 cgtgttgaca cggtcaaaga cgaaggtcac ggaattggag gttctcgcga cgacatcgac
     3121 taagccttgt ttccaaatcg gggcgatgtt gagtccgggg aatttcttac ccaattccat
     3181 cgtgaaaagg acgtcctgaa tggtgaaggg tgtgccgtcg ttccagacta caccgtctcg
     3241 gagcgtaaag gtcagctctt tcaggtcgtt ggaccactcg taggctgttg ccagccaggg
     3301 atggaatttt ccggtgcctt ggttgtaaaa caccagggtt tcgtaaaaac agccaactgc
     3361 gacctccaag gagttcggag agaaggggtt gaagttctgg atcaaaaccc cctgcttcat
     3421 cggggtcacc cgcaacatcc cgccgcgttt gatcgcttcg gcgccaaaca gactgctcaa
     3481 gacgagcagc aacaagagaa ccactacacc tacttttttc atgccattcc tccctttcat
     3541 attttgtttt caccacgaag ggctttatat gaacctattc tttctctttc acatttgggg
     3601 aaatgccttt ttcaacaaga aggagccgaa ctgttctctt atgccattat aaactattct
     3661 aactgaaatt tcaatgaccc gaatggtcat tcagctatat cgttcgtatg cacaacaaac
     3721 cccgtgtggg tataataccg cggcccaaca aaaaaaggga aaaggaggaa gggcaaaagg
     3781 ccaccctccg aaagagctat aacaacaagc cggattattc tgccacaaaa cgtgaggagg
     3841 aggggctcac cgggtctctt taccctattt cttgattaca ctccataact tgacccacag
     3901 cgacttctaa ccgtctcaat ccggtatggt ataatcaaag ccattcagca atcgtaccac
     3961 gtcgagttct tcgccacagg ccgagcgcac tgtgggatgg agaaccgacg tatataccta
     4021 accggacgct tcgctccaga ccgcgtgtac tggagggagg aggccggttt tttattgtga
     4081 ggtaacaaca tgaagaccgc cgcatacgcg cgcgtatcat ccgcccagca aagagaaaaa
     4141 aaggggggtc ccacggaggg cacagagcaa aggtactttt aaacgccata gggaaaccga
     4201 actttggttt ggcacgtctt ggtttcttgg gagcaccctg tgaagtctta agtgcttaca
     4261 ccacggttac tacaggttca tcgcggggta agtgtatttt acgctcccac atccgccttt
     4321 tgtccttttt acaaagctac acgctcagct ttttctccgt ttttgtatcg aaagtatcag
     4381 aaacgggtag gcagaaagtt ccgaagcgta tcgcgccgtc gcgaagacga aaattacata
     4441 cgccgcgatc tatgttctct ccgcgttctc cgtgccctct gtgttctccg tgagaaacac
     4501 tcttctcttc taatttaaca tgccgcacta aagtgcggcg ttcccaccca tctcccggct
     4561 cagctctttg cggatatctg ggacggagtt gtcgatctct ttttgaaaga gccgtcgaaa
     4621 gaggagttcg taggttttgt ctttcgcgat cacgtatcta gatgcggtgc tcccatatga
     4681 gattagatcc gctttgatta acttttgcag cttcgttttc agttctgaat cggttatatc
     4741 gagcttgagg tctttgatta ttcgcgctcg agtgcgttct ttgcccgcct gaaacaggta
     4801 cagcataatc cgtttcgcat ccctttcgtt cgcttccaag aaaactcctg cgaggtattc
     4861 gccccaagtt ttgaaaatca gtccgctctt gatctcttgc gtgtacactt cgatgacgtt
     4921 gtcctctcga tcgtaatctt tttttgcgtt gtggtatgag gagaaaagcg catagatata
     4981 caaagggtct ccacgtgtga gattccaaag cttgtccact ccggcattgt cgatctttgt
     5041 tcgatagatc tccgcgtact tccaaatcgc ttctttcgcc tcctcttccg ggaggttctc
     5101 cagg
//
LOCUS       DAOPXN010000166         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16524_length_5077_cov_4.801274, whole genome shotgun sequence.
ACCESSION   DAOPXN010000166 DAOPXN010000000
VERSION     DAOPXN010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16524_length_5077_cov_4.801274"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            312..821
                     /locus_tag="PKM49_08425"
     CDS             312..821
                     /locus_tag="PKM49_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNY82858.1"
                     /translation="MSLSIQMRETIGNMHLRGVDSIDPFLFHRTDLTSELIALGFLPT
                     SFHSTSSVTVFVSTLGIRIDEEIQRDFVEGRTLEGLLLDAWASEAIEAINDLFDAYIR
                     ENRSGTRRFSPGYGDVDIRKNHDILGYLTSEALTQGGCPLSHRISVNPSTGIMIPRKT
                     TIAMIGWFS"
     gene            844..1737
                     /locus_tag="PKM49_08430"
     CDS             844..1737
                     /locus_tag="PKM49_08430"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HNY82859.1"
                     /translation="MSERIVDIFENPLPSYSIEVFPPKPNLEKTLEIIFDTIDRLKVI
                     DPSFVSVTYGSGGYNRERALSIAGHLIQGAYRALSHITAVGYLRSDIVDLLDQLYYLG
                     VRNVLALRGDIPPNLIFPDTELDRFRYAYDLIDFVKRDGRFCIGAAAYPEGYADSGDV
                     DLSVLYLKKKAEVGADFFITQLFFDNARFYRFLEKARAVGISQPIVPAIMPILRNTNL
                     KRILSLSSTSLPSGLQEKIDRFGADPLDLEKVGIAHAAKQVQDLREHGMKHIHLYTMN
                     KSKQIIEICRLSETVSKSEGR"
     gene            1734..4064
                     /locus_tag="PKM49_08435"
     CDS             1734..4064
                     /locus_tag="PKM49_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HNY82860.1"
                     /translation="MKRKTFLSSLENRCLLLDGSYGVQLLMRGYRDSPAETYNVRQPE
                     VVATLQREYVACGVDLLSTNTFNSNPRKLKTLGFGEDFERFNRAGVRIAKEAARDKAR
                     VFGNMSATGEFVFPLGGYTFDEAVETFRRQAAILYEEGVEGFLLETFSDIKELKAAIM
                     AVRKVTADLPLIVNMTFEADGRSITGTSAESYAVSIQDLDVDVIGINCTLAPAQSLRV
                     FERLARVCQKPLCIQPNAGTPIYDGHRLSYDLSSESFAFYADDFIELGASIIGGCCGT
                     GPEHIRLMRRALDQRRSPRAIDKDPKRYLSSRTVLTPIQPFLSIGERINPASRKSLQE
                     QIQRKEWSQLLLEAQRQAEEGANAIDLNLGIEKTLDESAFSQVVCELDKRSSVPISFD
                     IQTDKFLEVALREYPGRPLINSARVTPKSLENKIALLKRYGGTLILLAMGKEIPDTLE
                     ERVHIAFEGIRILEENGITKDRILVDPLVLSMASGKDPKVTLGIIARLKEHGILTSIG
                     LSNLSYGLPNRTGVNGAFLAQAIERGLSAAILNSGDEVIQKVLAGALSLEGLDEPGIR
                     ASTIEDPILAAMVSGNREALNTLVEELLREHPPLTVSQQYLAKSMEELGQLYAKNEIY
                     LPQLLLASDTAREIFDLVNQRLDPLLVFRGRVLLATVEGDVHDIGKNIVGTVLRSGGF
                     EVIDVGKDVAAEKIVFKAKETDPDIIGLSAMMTTTIGEIETVIRALRENGIDKPVIAG
                     GASLNRELAKRFGCAGFAKDAVEGLELCKSLITRYR"
     gene            4191..4751
                     /locus_tag="PKM49_08440"
     CDS             4191..4751
                     /locus_tag="PKM49_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015218925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNY82861.1"
                     /translation="MIDTILLPVTLAFDAFAVAVCYGTTLPKTGFQGTFKVAFSFAAF
                     QFAMPVIGWHAGSSFQSAIREFDHWIAFGLLLYVGLGMIRDSRKVGTCPEKSDLSRLS
                     VLLLYALATSLDALAAGVALSMIAKPIWLPATIAFASTFVLSLAGVVSGAKTGASFGM
                     KAGLYGGFILIGIGTKILAEHLFFGG"
BASE COUNT         1224 a         1326 c         1353 g         1174 t
ORIGIN      
        1 ttaccccctt taatattcgt atggacaagc aaattataca actttttccg cgaaaccgta
       61 tcgtaaggct tgtgtatcat cataatcgac cacctctata aatcgaacct ctcctcgagc
      121 ccttcgagtg aagaagtgtt caggcacgac gttggtagcc ttcccgtttt atgctatact
      181 acggctagcc gtttcggctc tatcaaaaac cgtggattgg gtgatccgtg ccttttttcg
      241 aaatccaaaa agaccgcgtt gtaccagaca gacgccgatt cttatcgagg gctgggttca
      301 aaggaaacaa cgtgtcgtta tcgattcaaa tgagggaaac cataggaaat atgcatttac
      361 ggggtgtaga ttcgattgac ccgttcctgt tccataggac cgacctcacg tctgagctta
      421 tcgcgcttgg gttcctgcca acctctttcc attcgacgag cagcgtgacc gtttttgttt
      481 cgaccttggg aatccgaata gacgaggaga tccaacggga tttcgtcgaa gggaggaccc
      541 tcgaggggct ccttctggac gcttgggctt cggaggctat agaggcgatc aacgatctct
      601 ttgacgccta tatccgcgaa aaccggtccg gaacccggcg cttctcaccc ggatacgggg
      661 acgtggatat ccggaaaaac cacgatattc tgggctacct tacctcggaa gcgttgacgc
      721 agggcggctg cccgttgagt catcggatat ccgtgaaccc atccaccggc attatgatac
      781 cgagaaaaac gacgatcgcg atgatcggat ggttttcttg aatgtgatat acagggagga
      841 actatgagcg aacgcatcgt cgatattttt gagaaccctc ttccttcgta ttccatagag
      901 gtttttcctc caaaacccaa tttggagaag actctggaga tcattttcga tacgatcgac
      961 aggctgaagg ttatcgatcc gagcttcgtc agcgtcacct atggctcagg agggtacaat
     1021 cgcgaacgcg cgttatcgat cgcgggacat ctgatccaag gggcctatcg cgccctatcc
     1081 cacattacgg ccgtcggcta tttacgctcg gatatcgtcg atttgctgga ccagctttat
     1141 tacttagggg tgcgtaatgt tctggcttta cgtggagata tcccgccgaa cctgattttt
     1201 cctgacacgg agttggatcg ctttcgttat gcctatgacc tgatcgattt cgtcaaacga
     1261 gacggccgtt tttgtatagg cgctgcggcc tatcccgaag ggtatgctga ctcgggcgat
     1321 gtggatcttt ccgtcctata cctcaaaaaa aaggccgaag tgggtgcgga ctttttcatc
     1381 acccaactct tttttgacaa cgctcggttt taccgttttc tagaaaaggc ccgggccgtc
     1441 ggtatctctc agcccatagt tccggcgatt atgccgatcc tcaggaacac caacttaaag
     1501 cggattctta gcctctcttc cacctcgctc ccctcagggc tacaagaaaa gatcgatcga
     1561 ttcggagcgg acccgctcga tctcgaaaag gtcggcatcg cgcatgccgc caagcaagtc
     1621 caggacctcc gagagcacgg gatgaaacat atccacctgt acacgatgaa taaaagtaaa
     1681 cagatcatcg aaatctgtcg attgagcgaa acggtttcga aaagcgaggg aagatgaaaa
     1741 gaaaaacctt tctgtcgtcg ttagaaaatc ggtgtctcct gttggacggc tcctatggcg
     1801 tgcagctgtt aatgcggggt tatcgagaca gtcccgcgga gacttacaac gttcgacaac
     1861 cggaggttgt ggccacgctg cagcgggaat atgtcgcctg cggtgtggat ttattgtcga
     1921 cgaacacctt caactcgaat ccgaggaagc tgaagaccct cggttttggt gaggatttcg
     1981 aaagattcaa ccgggcagga gttcgtatcg cgaaggaagc ggcccgggac aaagcgcggg
     2041 ttttcggaaa catgtccgcg actggagagt tcgtcttccc tttaggcggc tacaccttcg
     2101 acgaggccgt ggaaaccttc cgccgacaag ccgctatctt atatgaagaa ggggtggaag
     2161 ggtttcttct tgaaaccttc tcggacatca aagagctcaa agctgcgatt atggcggtca
     2221 gaaaagtaac cgccgacctg cctttgatcg tcaatatgac cttcgaagcc gatggacgaa
     2281 gcattaccgg cacatcggcg gagagctacg cggtgtcgat ccaagacctg gacgtcgatg
     2341 tgattggcat taactgtacg ctcgcaccgg cgcagagcct acgggttttc gaacgactcg
     2401 cccgcgtctg ccaaaaacca ctctgtatcc aaccgaacgc cgggaccccg atatatgacg
     2461 gtcatcgcct ctcatacgat ctaagctcgg aatccttcgc cttctatgcg gatgatttca
     2521 tagaactcgg cgcttcgatc atcggaggat gctgcggtac gggacccgaa cacatccggt
     2581 tgatgcgccg cgcgctggac caacgtcgga gtccccgcgc gatcgataag gacccaaagc
     2641 gctacttgag ttcgaggacg gtgttgactc cgatccaacc cttcctatcg atcggagagc
     2701 gtatcaaccc ggcctcgaga aagagccttc aagagcaaat ccagcgaaaa gaatggagtc
     2761 aactcctgtt ggaagcccag cgtcaggcgg aagaaggggc aaacgcaatc gacttgaact
     2821 tgggaataga aaagacactc gacgaaagcg ccttctcaca agtcgtctgc gaactggaca
     2881 aacgctcgtc ggttcccatc tccttcgata tccaaacgga caagttcctc gaggtcgcct
     2941 tacgggagta cccgggccgg ccgttgatca actctgcaag ggtaacgcca aagagcctgg
     3001 agaacaagat cgccctcctc aaacgatacg gaggaacgct gatcttgttg gcgatgggaa
     3061 aagagatccc cgacactctc gaggaacgcg ttcatatcgc tttcgaagga atcagaatcc
     3121 tggaggagaa cggcattacc aaagaccgga tcctcgtcga tccgctggtt ctctcgatgg
     3181 cttcgggaaa ggatccgaag gtgacgttgg ggattatcgc gaggttaaag gaacacggaa
     3241 tcctgacgtc cataggccta tcgaacctca gttacggatt acccaaccga acgggcgtga
     3301 acggcgcctt tttggctcaa gcaatagaaa gaggcctgtc agcggctatt ttaaactcgg
     3361 gagacgaagt gatccaaaaa gtgcttgcgg gcgccttgtc tctcgagggg ctcgatgagc
     3421 ccggtattcg cgcgtccacg atcgaagacc cgatactggc tgcgatggtc tcgggaaatc
     3481 gagaggctct gaataccttg gtggaagaat tgttgcgcga gcatcctccg ctgaccgtca
     3541 gccagcagta tttggcgaag agtatggagg aattgggcca gctctacgca aagaacgaaa
     3601 tctatctacc tcagcttctg ctcgcctcag acacggcccg tgagatcttc gacctcgtca
     3661 accaaaggct cgaccccttg ctcgtgttta ggggaagggt tttgttagct acggtagagg
     3721 gagacgtcca cgacatcggg aaaaacatcg tcggaacggt cttgcgaagc ggcggtttcg
     3781 aggtcatcga cgtgggcaag gacgtagcgg ccgaaaagat agtttttaag gcgaaggaaa
     3841 cagatccgga tatcatcggg ctttcggcga tgatgaccac cacaatcgga gaaatagaga
     3901 ccgtcatccg agccctacgc gaaaacggta tcgacaaacc cgtgatcgct gggggcgcgt
     3961 ccctaaacag agagttggcc aaacggttcg gatgcgctgg ctttgcgaaa gacgcggtgg
     4021 aaggattgga actgtgcaaa tcgttgatca cccgttaccg atagaaaaga agagcgcggt
     4081 ggcgtcgtga ctctcgcgct tccctatcga tccgaagccc tcgatcccgc tccaaccgtc
     4141 cttctaaaaa tgacttcacg gggtatgcat aaggagcgca gaagattacg gtgatcgaca
     4201 ccatcctttt acccgtcaca ttagctttcg acgcgttcgc cgtggcggtc tgctatggga
     4261 cgacgttacc aaagacgggg tttcaaggca ctttcaaagt cgccttttct tttgcggcgt
     4321 tccagttcgc gatgcccgtg atcgggtggc acgccggatc gagtttccag tctgcgatcc
     4381 gcgaattcga ccactggata gccttcggtt tactcctata cgttggcctc ggtatgataa
     4441 gggactcccg aaaagtcggg acctgtccgg aaaaatcgga tctatccaga ctatccgttc
     4501 tccttctgta cgccttagcc acaagcctcg acgccttggc tgccggcgtc gcgctttcta
     4561 tgatcgcgaa gccgatatgg ttacccgcca cgatcgcttt cgcctctact ttcgttttat
     4621 ccttggccgg tgtggtttcg ggcgcgaaaa ctggcgcaag ttttggcatg aaagcagggc
     4681 tctatggcgg attcatattg attggtatcg gaacgaagat cctggcggag cacctattct
     4741 tcgggggctg agcggtagaa cgcggtctaa aaacccttgc gcgcgggccc tcctgacccc
     4801 gcaccagcct cgaataccag gtaggcatat tcttcgctct ggggtcacca aagttattta
     4861 acctacgcac aaatcctctc aaaaacaccc tccccacatt ctggcttcgt cacaagtcac
     4921 attaacagag cgttacttat tgtttactta tcggaaaagc tatcagataa ttagctgtgt
     4981 taaaaccaga ggggtatggt ataatttcct gaagatttcc gaagggggtt ggaaatgaga
     5041 gtccttgttt tagaggatga cttggtgaca ggaaaac
//
LOCUS       DAOPXN010000167         5063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16594_length_5063_cov_5.200280, whole genome shotgun sequence.
ACCESSION   DAOPXN010000167 DAOPXN010000000
VERSION     DAOPXN010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16594_length_5063_cov_5.200280"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(545..2563)
                     /locus_tag="PKM49_08445"
     CDS             complement(545..2563)
                     /locus_tag="PKM49_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82862.1"
                     /translation="MIKIRSIKARLFLYTIPAILIIFTIGALILATLSKRNVYSLFSN
                     QTVQTTTAIELYMSEWIDGIKKHLYDISRSAEITSMDSKLHHPRLADMVKNGKGLYEY
                     IFVADLAGNFINFDGSMASIQERPYFKEIVNQRKDVAMSDASISKGTGLPVVMIAMPI
                     HKEGGALVGVLVAALNIDSLSKKLSAFRFGETGYAFMIDRLGVCIAHSADEKLVMALN
                     VNNVESQGFAGLKELERRAMSGESGAGEYTRPDGGLMHLFFRPIRGTPGWSLSLSMPD
                     KELNASTVTMVLLVLLVFVSVLVVIVWIIFFTGTSISRPIVRVTKALERLGDLDLSSR
                     DSVIEQYTARKDEIGKISLAMNKMAEAVRASVSLIRISVNETNNAADALDKVSEGQLR
                     AAQALAEQAEVVDRHVQNTSAAVEEVTSGVEEVAASAQGLSHTAQDLSTTSEKALAIV
                     REGTEAVREVIERVQEAWRQTEDSAKQATQVAILTGKVSEITGTIESISSQTNLLALN
                     AAIEAARAGEAGKGFAVVADEVRKLAEESKNATNEIGVILKQVAEGVAKADASTEDTL
                     NLVKRVYDSGLEIERQFQEISKKVDGIYSMVAHFTATAEEQGAATEEMASAMETSSKS
                     MLDISDKVQAMNESAKTQRSQADEVHISAEQLSRLAENLRTEIAKFKT"
     assembly_gap    2603..2702
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3148..3462
                     /locus_tag="PKM49_08450"
     CDS             3148..3462
                     /locus_tag="PKM49_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82863.1"
                     /translation="MERANRNLYTLGTGIFFLGLAVIFFVKEIHFWPWILALIGLAWL
                     PWSLATKKGWYGWNGCVWLVGLALLFHFNVFWPGILILIGISIAFGSLTGKTTGCPYK
                     KH"
     gene            complement(3499..4626)
                     /locus_tag="PKM49_08455"
     CDS             complement(3499..4626)
                     /locus_tag="PKM49_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNY82864.1"
                     /translation="MKLRIWLRWVTAIGILLSISGCTVLYRIYDPLNPIPAAESRTSA
                     TTVCLRWEYPEVSEVTFDVYLGTSASRMELIARDLKETCVTVGPLLTNTRYYWKVIVR
                     NAFGQYRSGRVWFFDVIGGKHYSLLIGVSDYDVGSDLRWTVNDAQDLQTCLEHLSYEY
                     INRQLLTRVVEEDVVAFFDELEPLFTDGDTLLFYFAGHGSYDFATSESYLLLTNGSKI
                     TVSELRGLINRLEGTVLVVLDSCRSGGFTNLTASREEIFADLDRYNRSFIEGLTVGAR
                     GAGTYVLTAAKHTESSWEDAVLQNGVFSFFLADGLGHTGLSAPMGAFDSTYDADADGD
                     ERITLDEIYQYTQKNVNSYLKGIQQVQVFPVNSDYVIAQWD"
     gene            complement(4854..>5063)
                     /locus_tag="PKM49_08460"
     CDS             complement(4854..>5063)
                     /locus_tag="PKM49_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82865.1"
                     /translation="RCFLRGIERDRQAVDLGIGKGYGNDLSRDFVGFLSLRAGYPAIT
                     PRGHHGVSASSFRDGSMRFRTTIAV"
BASE COUNT         1195 a         1309 c         1228 g         1231 t
ORIGIN      
        1 gcgcaacaag gcctggagca gatcaaagag aaggggtatg cgaacaggta taaagatcgg
       61 tcattgttcc tcgtgggtat cggaatcgat gaggagaaac gaaatctcgg ctgttatgcg
      121 atggagaggg tttcttctta aaacgcggga aaaaggtttc gtacggaggg gaaagaaaaa
      181 aagggttcgc acagagaaca cagagggcgc ggagaataca gaggaaaaac tggcaagggg
      241 caaggcgctc ccggaaaaag aagacatgcc ggactaaagt ccggcgttcc cagggcgttc
      301 ccgggacgtc ttaataaaca aaaggcgtat cgcgtgactt cgtgagcttc cgaagtcagc
      361 ccgctggtta ccccgcgatg aaccttttag ttatgtcact ccataacttg acccacagca
      421 acgaaagacg acgcggaagc gtgactgtcc gtggttctaa ccaactaagt agtccaaact
      481 gcgttcaacg aggaacgatg aaatcgttgt tgacgtatca gtttatgggt tcgctttttg
      541 tcgattaagt cttaaatttc gcaatttcgg ttctcaggtt ttcagccaat cggcttaact
      601 gttctgcgga aatatgcact tcgtccgctt gtgagcgctg agtttttgcg ctttcgttca
      661 ttgcttggac cttatctgaa atatcgagca tagatttgga agaggtttcc atcgcgctcg
      721 ccatttcttc cgtggcggcg ccctgttctt ccgcggtggc tgtaaaatgg gccaccatcg
      781 aatagatgcc atccactttt tttgaaatct cttgaaattg cctttctatc tccagcccac
      841 tatcgtagac ccttttcact aaatttagag tatcttcggt agaggcatcc gccttcgcca
      901 ccccttcggc gacttgtttc aagatcacgc caatctcgtt tgtggcattt ttactttctt
      961 cagcaagctt tctaacctcg tcggccacga ccgcgaaccc tttcccggct tcaccggctc
     1021 gcgcggcttc tatcgccgca ttaagcgcga gtaaattcgt ttggctcgag atcgactcta
     1081 tcgtcccggt gatctcgctg actttacccg tgagtatggc cacttgcgtc gcttgtttcg
     1141 cggagtcttc cgtttgtctc caagcctcct ggacacgttc gatgacttca cgtaccgctt
     1201 cggtgccttc gcgaacaatg gcgagcgcct tttccgaagt cgtcgaaagg tcttgggccg
     1261 tatgagaaag tccttgcgcg ctggcggcaa cctcctccac gcctgaagtc acttcttcga
     1321 ctgcggcaga ggtgttctga acgtgacgat ccacgacttc agcttgttcg gcaagcgctt
     1381 gggccgctct caactggccc tccgaaactt tgtccaaagc gtccgcggcg ttattcgtct
     1441 cgttgaccga aatacggatg agagagacgg acgcgcgaac ggcctccgcc atcttgttca
     1501 tagccagtga tatcttcccg atctcgtcct tgcgagccgt atactgttcg atcacggaat
     1561 ctctacttga taggtcgagg tctcccagtc gttccaatgc tttcgtcacc cgaacaatcg
     1621 gcctcgatat cgatgtgccc gtgaagaaaa tgatccatac gataacgact aaaacgctga
     1681 caaagacgag aagaaccaac aagaccatcg tgacggtact ggcgttcagc tccttatcgg
     1741 gcatcgagag ggagagcgac caccccggag ttcctcgtat cggacggaaa aacagatgca
     1801 taagaccgcc atctgggcgg gtatattcgc ccgcgcccga ttctccactc atcgcgcggc
     1861 gctcaagctc cttgagccct gcaaagcctt gcgattccac gttattcacg ttcaaggcca
     1921 ttactagctt ttcgtccgcg ctgtgggcga tgcagacgcc caaccgatcg atcataaatg
     1981 cgtaaccggt ctctccgaaa cgaaaggcgc tcaatttttt cgaaagcgaa tcgatgttca
     2041 acgctgcgac caaaacgccc accaaggcgc ctccttcttt atggataggc atcgcgatca
     2101 tcacaactgg caacccggtc cccttgctga tcgacgcgtc gctcatcgcc acatctttcc
     2161 tctgattcac aatttcttta aaatacgggc gctcttggat gctcgccatc gatccatcga
     2221 aattgatgaa gttcccggct aagtcggcga cgaagatgta ctcgtataga cctttcccgt
     2281 tcttcaccat atcggccaac cgtgggtgat gtagcttcga gtccatcgag gtgatctctg
     2341 cgcttctgga gatatcgtat aaatgctttt ttatcccgtc tatccactcg ctcatataga
     2401 gttcgatggc ggtcgtcgtc tgaactgtct gattggaaaa caagctatag acgttccgtt
     2461 tcgacagcgt ggcgagaatg agggcgccaa tcgtaaaaat gattaagata gccggaatgg
     2521 tatacaaaaa aagccttgcc ttgatcgatc tgattttgat catcctgcgc gacctccgtc
     2581 gtgtatactc cttttcctat ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnagatatgg caaataaaaa aagaggtgtt aaaaatactc gcaaggcgag gcgatacaca
     2761 gaaacgaaac gtgaatgaaa ccgaacggcg cggttgagcg accttctagt tgacggtttt
     2821 ttagcgtacc tttctgggag cgccgcattt cagcgcgaca tagttttata tatttcgccg
     2881 ttcagaaagg tataagaaag ttataagata gttgagccga actttagtcc ggcgctgggt
     2941 atttggttag gacaggcatt ttgtgataca ctatgaaaga gacgagaacg tcgttcggaa
     3001 aagaatccgc taaatcgcgg ccgaccgggg gcgcttaaaa aataggggtt ttgaaccact
     3061 gcgcccgctg atagacgatg tgacgctcta tctgtaaaaa aaggcatctc ggcatcataa
     3121 gacccctgtc gaagaaagga gagtacggtg gaaagagcaa atcgaaacct gtatacacta
     3181 ggaaccggaa tattcttttt gggtttagcg gtgatttttt tcgttaaaga aatccacttt
     3241 tggccttgga tactcgcctt gatcggactg gcttggcttc cctggtctct ggccaccaag
     3301 aaaggttggt acggttggaa cggctgcgtt tggctggtgg ggttggcttt gttgtttcac
     3361 tttaatgtat tctggcctgg tatcttgatt cttatcggga tcagcatcgc ctttggctca
     3421 ttgaccggaa agacgacggg gtgcccgtat aaaaagcact gaggttttgg aactacgctg
     3481 aagacaagcc ccaaaccgtt aatcccactg cgcgatgacg tagtcggaat tgacgggaaa
     3541 aacttgcact tgttgaatgc ccttcaaata agagttcacg ttcttttgag tgtattggta
     3601 gatttcatcc aacgtgattc gttcgtcgcc gtccgcgtct gcgtcatagg ttgaatcgaa
     3661 ggcgcccata ggcgccgata acccggtgtg gccgagtcca tcggcgagaa agaaagaaaa
     3721 gactccattc tgcagcaccg cgtcctccca gctgctttcc gtgtgcttgg ccgccgttag
     3781 aacatatgtc cccgcccccc tggctcccac cgttaaccct tcgatgaaag aacggttgta
     3841 tcgatccaga tccgcaaaaa tctcttctct cgatgccgtc aaatttgtga aaccaccgga
     3901 gcggcaggag tccaacacca cgagaactgt gccctcaagg cggttgatga gtccgcgcaa
     3961 ctccgagacc gttatctttg agccgttcgt caataggagg taggattccg aggtggcgaa
     4021 atcgtatgaa ccgtgaccgg caaagtagaa caatagggtg tcgccatccg taaaaagcgg
     4081 ttccaattcg tcgaaaaaag ctaccacatc ctcctctacg acccgggtca gcagctgccg
     4141 gtttatatac tcgtaggaca aatgctccaa gcaggtttgt aaatcttgcg catcgttaac
     4201 cgtccatcgt aagtcggagc cgacgtcgta gtccgaaacg ccgattaaca gcgagtagtg
     4261 cttacctcca atcacatcga aaaaccacac tctgccggac cggtactgtc caaaggcatt
     4321 tcgaacgatc actttccagt aatagcgggt gttggtcaac aacgggccga ccgttacgca
     4381 agtttctttc aaatcgcgcg cgatcagctc catcctgctc gccgaagtcc ccaagtagac
     4441 gtcgaaggtc acttccgaca cctccgggta ttcccaacgc aggcaaaccg tcgtggcgct
     4501 ggtacgactt tccgcagccg gtatcggatt caacggatcg tatatcctat ataacaccgt
     4561 gcaccccgaa atggacaaca gtatcccaat tgccgttacc cagcgcaacc atatccttag
     4621 cttcatataa tccccctttt tccgacagaa ccgtcagact cgaggtctca ccgtctatcg
     4681 gtagcggcaa gccgattcca tcgttccgcg cgcacgttta ttatatccta tcttgagaag
     4741 cctaagcaaa tatcgggcga gtataatatc ggggtacacc aaattgggaa cgccgaactt
     4801 tagttaggcg tgtttttttg cttttcagta agctctcgag agcccttaaa cgcttacact
     4861 gcgatggtcg tacgaaagcg catcgagccg tcgcgaaaag aagaagcgct tacgccgtga
     4921 tggccgcgtg gggttatcgc ggggtaacca gcgcgcagac ttaagaagcc cacgaagtct
     4981 cgcgataggt cgttgccata cccctttcct atgcccaaat cgaccgcctg gcggtctctt
     5041 tctattcctc gcaggaaaca tcg
//
LOCUS       DAOPXN010000168         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_16639_length_5052_cov_5.200520, whole genome shotgun sequence.
ACCESSION   DAOPXN010000168 DAOPXN010000000
VERSION     DAOPXN010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16639_length_5052_cov_5.200520"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            830..2851
                     /locus_tag="PKM49_08465"
     CDS             830..2851
                     /locus_tag="PKM49_08465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HNY82866.1"
                     /translation="MEPLKMDDLTKYTFLSGPALSPDGETCAFAAHQMDLAENEYRSD
                     IWCYRLSDRKLTQMTTSHKDGRFIWLSDGRRLLFSSLREKEDIKAKESGEVFTQFYTI
                     SLDGGEAQKAFRVPFPVSSITEINPDLFLLEIVWWPPFETLAGAAAAEKPKLLQSMKE
                     ETDYEVLDEIPFWSNGEGFTNKKRTRLYLYDVKTKDLSPITGETFLAETPQLDETHER
                     FAFTGIDFSDKLEIRNDVYLYTLKDKSLEKFSMGETFLTDYVDFWDKDTLFVVGQEQR
                     NYGINENPKFYAFCLKDKTLRCLTPELDLAPGNALNSDCRYGGGSKPIQKDGEAFYFL
                     ATEGVGASFKKICPDGTVETVAQMKGSLDSFSIKKGKLAFIGFHELKLAELYTFSNQK
                     FEAVTDFNGWVLRERTLSNLERLVVKADEKHPTLATDIDGWLIRPVPFDPDKTYPGIL
                     MIHGGPKTTYGENFIHEMQWLANEGYAVFFCNPRGSEGRGNAFADVRGKYGTIDFDDL
                     MAFTDEVLERYSFLDPARIGVTGGSYGGFMTNWIIGHTDRFRAAVSQRSIANWVSKFC
                     TTDIGYFFVPDQNSATPWSDVDKLWEHSPLKYADRVKTPTLFIHSEQDYRCWLPEGIQ
                     MFTALKYHGVDSRLCLFRGENHELSRSGKPKHRLRRLKEIKEWFDRYLK"
     gene            3503..3577
                     /locus_tag="PKM49_08470"
     tRNA            3503..3577
                     /locus_tag="PKM49_08470"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3535..3537,aa:Asn,seq:gtt)
     gene            3992..4858
                     /locus_tag="PKM49_08475"
     CDS             3992..4858
                     /locus_tag="PKM49_08475"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNY82867.1"
                     /translation="MNSLKIILDSTGDIPFEWAAKIDVENIPLHVLWDQPSETEDDFR
                     DQEQINQFWEKLQARPQPPKTSQPTPGEFSTLYKKAFDEGYSEILALCISAKMSGTYN
                     SAEIAARDYPGNIEIVDSKMASAANALLARRARELADQKVSARRIKDILDREIKERRF
                     GAYFYVSDFEFLKKGGRISGFASFIGSMLKLKVALHLSEDGILVPFAKNKGVEKTHDS
                     LIKAALSLYPERAKVNLIMVYTIAADEARLLSEKLKKVYQVENLVFSPMGKVISTHVG
                     PYATGFGIERIL"
     gene            4924..4999
                     /locus_tag="PKM49_08480"
     tRNA            4924..4999
                     /locus_tag="PKM49_08480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Ile,seq:cat)
BASE COUNT         1283 a         1256 c         1309 g         1204 t
ORIGIN      
        1 gtggctcctt cgggggccgt ttttttccct cctcgttttt gttctttttt tttatcacac
       61 cccgatatga tacccacaca aaagcccttg agggttcagg atggtgtatt ccactcgaaa
      121 tcaaatgaat ggacgaaaaa tcgcgcgatc ggctctggcc ttgttttttg attacacccc
      181 ataacttgac gcatacgttt ttattaagcg gaaactaggg gtggagttcg ttgaattgag
      241 agcgcttggt cttcagatag gcgtattttc ttttttcaga cactctcagg aaaacctgcc
      301 aacttaaaag tcggcgttct ctgcccaaga agtctcgcga accgccctct cttttattca
      361 ataaacgtat cgcgccgtcg cgaaaagaag aagcgcttac gccgcgatgc tgcgcttcat
      421 ggcgttccca gaagagtcaa cccgaaaacc gtgccgcact aaagtgcggc ggtcccagcg
      481 acgttctctg cccaagaagt cccacgaacc gccctctctt ttattcaaag agcgtatcgc
      541 gccgtcgcga aaagaagaag cgcttacgcc gcgatgctgc acttcagggc gttcccagaa
      601 gagccaaccc gaaaccgtgc cgcactaaat tgcggcggtc ccagcggcgc tttcagagtg
      661 aatcttatgt gtcctccgtg ttcttgatgc gttttttgat tcttcttctt agaattccca
      721 cttttcagcc ctcttgtgat aaactctaac ggtgcgcccg aaccgaagcg aaaagtatcg
      781 gacgagcgta cgaggcgttc actttttaca agaaaaggag atggatcgtg tggaaccatt
      841 gaagatggac gatttaacga agtatacctt cctttccggg ccagcgctct ctccggacgg
      901 agaaacctgc gccttcgccg cgcatcagat ggacctcgcg gagaacgagt accggtccga
      961 catctggtgt tatcgccttt cagacagaaa gctgacccaa atgaccacgt ctcacaaaga
     1021 cggcaggttc atctggttat ctgacgggcg acggctgttg ttttcttctc tgcgagagaa
     1081 agaggatatt aaagcaaaag aaagtggaga ggtgttcact cagttttaca ccattagcct
     1141 agacggcgga gaggcacaaa aggcctttcg tgtgccattc cccgtatcct cgatcaccga
     1201 aatcaacccc gacttgttcc ttttagagat cgtatggtgg ccgccgtttg agacgctcgc
     1261 aggggccgct gcggcggaaa agccgaagct ccttcagtcg atgaaagaag agacggatta
     1321 cgaagtgcta gacgagatcc cattctggag caacggagaa ggcttcacga ataaaaagag
     1381 aacgcgtttg tacctatacg acgtgaaaac gaaggactta tcgccgatca cgggcgaaac
     1441 cttcctcgcc gaaacacctc agttagacga aactcacgag cgtttcgcct ttacgggcat
     1501 cgatttttcc gacaaattgg agatacggaa cgatgtgtac ctttacactc taaaagacaa
     1561 gtctctggaa aagttctcta tgggagagac ttttttgaca gattacgtgg atttctggga
     1621 taaagacacc ctgttcgtcg tcggtcagga gcagcgaaac tatgggatta acgagaaccc
     1681 aaaattttac gctttctgtt tgaaagacaa gaccctacgg tgtctcaccc ccgaactcga
     1741 tcttgcccca ggaaacgcgc tgaattccga ctgtcgctat gggggagggt caaaaccgat
     1801 acaaaaagac ggggaggcct tctatttcct cgcgacggaa ggggtcggag cctctttcaa
     1861 gaaaatctgc ccagatggca ccgttgaaac cgtcgcccag atgaaggggt cgctcgatag
     1921 cttctcaata aagaaaggga aattggcctt tatcgggttt cacgaattga aactcgccga
     1981 gctgtacacc ttctcaaatc agaaattcga agcggtgacc gacttcaatg gttgggtttt
     2041 aagggaacga accctatcca acctggaaag acttgtggta aaagcagacg aaaaacaccc
     2101 cactctcgcc acagatatcg atgggtggtt gatcagaccg gttccttttg atccagacaa
     2161 aacctatccg ggaattttga tgatccacgg aggtccgaag accacctacg gagagaactt
     2221 catccacgag atgcaatggc tcgcaaacga aggatacgcc gtattcttct gtaacccgag
     2281 gggtagtgag ggtcgcggca acgcgttcgc cgacgttcgt ggaaagtacg gcacgatcga
     2341 tttcgacgac ctcatggcct tcaccgacga ggttcttgag agatattcct tcctcgaccc
     2401 cgcgcgtatc ggcgtgacgg gcggttcgta cggcgggttt atgaccaatt ggatcatcgg
     2461 ccacaccgac cgatttcgcg cggcagtttc acaacggagc atcgcgaact gggtatcaaa
     2521 gttttgcacg accgacatcg gctacttttt cgttcctgac cagaatagcg cgacgccttg
     2581 gtccgatgtc gacaagctct gggagcactc ccctctgaaa tacgcggata gggtgaaaac
     2641 gcccactctt ttcatccact cggaacagga ctaccggtgt tggttgccag aagggatcca
     2701 gatgtttacc gccttgaagt accacggggt agactcacga ctctgccttt tccgtggaga
     2761 aaaccacgaa ctcagtcgaa gcgggaaacc caaacatcgc ctcagaaggc tgaaagagat
     2821 caaggaatgg tttgatcggt atctgaaata gaatactccc gcagtgtgag aataatagcg
     2881 tatcgtgccg tctggaaaag aagaggcgca tacgccgcga ttgctgcacg ggtcaccgcg
     2941 gggtaaccag cgggctgact tcggaagccc acgaagtctc gcgatacgct ctgggaacgc
     3001 cgaactttag ttcggcatat cttttcaaag agcaaaaaca gggttcacac agagggcaaa
     3061 gagagcacag agaagagatt cttgggaacg ccgtgtgcct gggtacgccg aactgcagtt
     3121 cggcgtgttt ttttgttttc acagacactc tcagtaaaga caagccaact taaaagttcg
     3181 tgttcacaga aaagccaacg ctaaagcctt gacgcactta gtgcagcgtt cacagaagga
     3241 cgttcccggt aaccgtgcca gactaaagac cggcgtgtct tttctcttcg atggtttttt
     3301 agaagtgtgc ttctctagaa aagccaaact tttattcgac gtgcctttgc ttttcaagcc
     3361 ttcgctggtg ggcgaaccta cgcgtgatct tttcttttaa ggctggaaga ggaccggaaa
     3421 cgaggctagg cgagaggttg caacaatacc gtcgagaccc cttgattttt ctggaaataa
     3481 atgatagaat ccggatgatc tttcctgcgt agctcaatgg cagagcatgt ggctgttaac
     3541 cacagggttg ggggttcgag accctccgca ggagccaaag ttaaagtccg ttaatcgtgg
     3601 cggcgcttcc ttgaaagaga gacctcgcgg gttcatttcc tcggggtcta tttttttcga
     3661 cccctgaggt ggagtacgtt ttgctcatcg catcgatccc ctttcccttt gtcggacgat
     3721 atgaaaagta tccattctgg gtttcgtgtc agaaaggcca aataatgatt tatgaaggtt
     3781 aggaaattgg gcttcgaaaa caccgcaagg ggcgtatgcg tcttgatgac agcgaaataa
     3841 aacggaacaa cgcgcttttc aggacggctc cacaggacat cgtacaggcg ttgtaggtac
     3901 tccgccttcc cttttccaga tacccacact caccgttcgt tacgatctat tgtgtgttat
     3961 aatcagaaaa atgttttcag gaggttgagt gatgaactct ttgaagatta ttttggacag
     4021 tacaggggat attccgttcg agtgggcggc caaaatagat gtggagaaca ttccgttaca
     4081 cgttttgtgg gatcaaccgt ccgaaaccga agatgatttt cgcgatcaag agcagatcaa
     4141 tcaattttgg gaaaaactgc aagcgcgccc acaacctcca aaaacctctc aacccacccc
     4201 tggcgagttt tctaccttat acaagaaggc tttcgacgag ggatactcgg agattctggc
     4261 gttgtgcatt tcagcgaaga tgtccggcac ttacaatagc gccgaaatcg ccgctcggga
     4321 ctaccccggt aatatcgaga tcgtagattc caaaatggct tcggcggcca atgccctgct
     4381 cgcgcggagg gctcgggaat tagcggatca gaaagtttcg gcccggagga tcaaggatat
     4441 tttggacaga gagatcaagg aacgacgttt cggcgcgtac ttttatgttt ctgatttcga
     4501 gtttctgaaa aagggcgggc gtatctccgg tttcgcgagt tttatcggaa gtatgttgaa
     4561 attgaaggtg gctttgcact tatcagaaga cggcattctg gtgcctttcg ccaagaataa
     4621 aggcgtggag aaaacccacg actccctcat caaagctgcg ctgagccttt accccgagcg
     4681 tgcgaaagtg aacctgatta tggtctatac gatcgcggct gatgaggcgc gactcttgag
     4741 cgagaagctc aaaaaagtct accaagtcga aaacctggta tttagcccga tgggtaaggt
     4801 aatctccacg cacgtcggac cctatgccac gggtttcggt atcgaaagaa ttctataagc
     4861 ggctcgcttg caatttcggg aagtctattg tataatacac ccgccgttta tcgacgacgg
     4921 acagggctct tagctcatcc ggtagagctc ccggctcata accgggcggt ggtaggttcg
     4981 agtcctacag agcccaccac gtatcggggc gtggcgcaga tggaagcgcg tcagcatggg
     5041 gcgttgaagg cc
//
LOCUS       DAOPXN010000169         4952 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17075_length_4952_cov_5.078211, whole genome shotgun sequence.
ACCESSION   DAOPXN010000169 DAOPXN010000000
VERSION     DAOPXN010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17075_length_4952_cov_5.078211"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1495)
                     /locus_tag="PKM49_08485"
     CDS             complement(<1..1495)
                     /locus_tag="PKM49_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82868.1"
                     /translation="MKIRSVRAKLLLYFIPVSVVVLIIGAVIVGVLAKDVTFEIGQNS
                     MESISDIAIIVIQEWITGIKNELRNLSITDVVRSFDPKRFNPRFQEAIQESKGLYETF
                     IVVDANGEVITGTDAKANIRDRSYFQEVMFQGKDFAMSNAVISGASNAPVITCVYPIF
                     DTAKKRVGIVGATVSLKALGDIVASINIGKGGFAFLTDGTGMLVAHKITDLVMQLNVT
                     EASRAGFKGLEELGREMVRGKSGSGTIALPTGERHIVFFDPVPNTPNWTLGEVMPEAE
                     AAESSIFLVTVVIVIFAVIIGIIILISLFTGAVISKSMKKLALQVDNFGKGDLTTTFE
                     AKGHDEIAQMAKSLSVMGHDLREAMIAIKNSETELNSASTDLAAISEEQLARTEELST
                     QSHSANKNLESTVAAIEEVNSGVDEVAASAQNVSKTAQALSHENEKTAEGSRKGGQMI
                     ADVMVEIEATTAQTQLTAEKVQKLAENSKNVGEIVDTISSIAEQTNLL"
     gene            complement(2343..4343)
                     /locus_tag="PKM49_08490"
     CDS             complement(2343..4343)
                     /locus_tag="PKM49_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82869.1"
                     /translation="MMKLKSVRSKLLLYFIPVSVSVLVIAGFIIGLNARSVILELSSD
                     STEETLHACVHIVEEMLAGIKTEMRNLSNTDVVKSFDPTRFCPRFIETIRQSGGLFET
                     FFLADPTGKAIMETGEIVDISDRPYFQDVMFRGKEFALNDGIISRFTNAPAFGAAYAV
                     YDDTGHVVGALGTTVALKILSNKVTSTKIGEEGYIFLVDGAGTVIAHPDTDKILKLNL
                     LEGTKSGYKGLEDAGKEMIRGSDGIKNIIFPSGEKYILHYDPIPNTPNWSVAGALSEA
                     EANQKSVYLLGIVIAVFVIIVAVIVFISLFVGTVISKSMKKLAVQVDNFGKGDLTTAF
                     EAKGHDEIAQIAKALGGMGHDLRAAMISIGGASKEIKAASTDLAAISEEQLAGSEELS
                     GQSASVNTNLQNTVAAIEEVDSGVEEVAASAQNVSKTAQALSTENDKTADGSRKGGQM
                     ISDVVREIESTTKQTLLTAEKVQKLAENSKSVGEIVDTISSIAEQTNLLALNAAIEAA
                     RAGEAGRGFAVVADEIRKLAEESKKATSNIGAILKEVQKDAEESYQATGQTVGLVKDV
                     NTKSQAVEQQFKHLLDMVEKTTAMIENLTATSEEQGAAAEEMASAMSTSAKSAHEISE
                     QIRQMSQGIEQQAQGAQQISASAQELDTLAESLDSQVKRFKV"
BASE COUNT         1098 a         1314 c         1148 g         1392 t
ORIGIN      
        1 ccagcaggtt tgtctgttcc gcgatagagg atatcgtgtc gacgatctcg cccacgttct
       61 tcgagttttc cgcgagtttt tgaacctttt ccgccgtcag ctgcgtttgc gccgtcgtcg
      121 cctcaatctc gaccatcacg tccgcgatca tctgcccgcc tttgcgtgaa ccctctgcgg
      181 tcttctcgtt ttcgtgagac aacgcctgcg ccgtcttcga cacgttctgc gcgctcgctg
      241 ccacctcatc cactccggag ttcacctctt ctatcgctgc gacggtgctc tccaaattct
      301 tgttcgcgct gtgggattga gttgacagct cctccgttcg ggctaattgt tcttcagaga
      361 tagcagccag atccgttgag gcggagttaa gctctgtttc agaattcttt atcgcgatca
      421 tcgcctctct gaggtcgtgg cccattacgg ataaggactt agccatttgg gcgatttcgt
      481 cgtgaccttt cgcctcgaat gtcgtggtca agtctccctt tccgaagtta tccacttgaa
      541 gggcaagctt tttcattgat ttggagatga ccgcgccggt aaatagggag atgagtatga
      601 tgatcccgat aataacggcg aaaatgacga tcacgacggt tacgagaaaa atagaagatt
      661 ccgcagcttc cgcttcgggc atcacttctc ctagagtcca gttcggcgta ttaggtacgg
      721 gatcgaaaaa tacgatatgt ctttcgccag ttggtaaggc tatagttccc gaaccgcttt
      781 ttccacggac catctctctt ccgagttctt ccaagccttt aaacccggcc cttgaggctt
      841 ccgtaacgtt aagctgcatc actaagtcag tgatcttatg agccacgagc atcccggttc
      901 cgtctgttaa aaaggcgaat ccgcctttcc cgatattgat cgacgccacg atatcgccca
      961 gggccttcag agaaacggtg gccccgacga tacctacccg ctttttcgca gtgtcaaaaa
     1021 tggggtagac acacgtgatt acaggcgcgt tcgatgcccc gctaatcacc gcgttgctca
     1081 tcgcgaaatc tttgccctgg aacataacct cttgaaaata tgaccggtct cttatgttcg
     1141 cctttgcgtc agttccagtg atgacttcac cattcgcatc cacgacgatg aaggtctcat
     1201 aaagcccttt gctctcttga attgcttcct gaaacctcgg gttaaatcgt ttggggtcga
     1261 aagacctgac aacgtccgtt atagacaggt tccgtaattc atttttgatc cctgttatcc
     1321 attcttgaat cacgataatg gcaatatcgc ttatcgattc catcgagttt tgaccgatct
     1381 caaaagtgac gtcttttgcg agtactccaa caataactgc gccgatgatc agcaccacga
     1441 ccgataccgg aataaaatac agtaacaact tcgcgcgcac acttctaatt ttcatgcaca
     1501 tccactcctt cgtaagcccg attgtgtgtt ttaaaaaagt ctttttcctt gaagcgctat
     1561 gtattttctt tggcttgaag tagcttgact aggctcactt agaaatctgc gcagcatcgg
     1621 ccacggggtt ctatgtatgg ccgcatcttt gacagcttct taaatgacct actcacccgt
     1681 gtactctcaa atgaagcaga gtgaggaaca cggccttttt gtttcattgt ctatagcttt
     1741 ttcagtcacc tggtttttcc cccttagggg ttaaagagta agttttctga aagaaggccg
     1801 gcgactcagt tggtcttctg agagtatcat atcacgaaag aatgtttttc tcaaaaagct
     1861 ataaaaagaa aacgcctgtt ctaaagatgt gtttaaacga atcgtattga attgagttat
     1921 gcaaacctat caatgggcca ccagttcttc gaaacgctct ccttcggtct cctgtgaaca
     1981 cctctgcggt accgccatag cgatggacgt ttgtctggcg ctgttttgtt tttcctggga
     2041 acgccacact ttagtgtggc atgggttgtg attattcttt gaaaaccgga aaccaagcca
     2101 acttgaaagt tggcgttccc agagagtttc caaagcgcta ccaaaaatcc caagaacatg
     2161 ccgaactgaa gttcggcgtt cccagcgcgt tcccagagag ttcccaaagt actgccaaaa
     2221 atcgcaaaat atgccgaact aaagttcggc gtttcctgtg cgttcccagg ccactcccgg
     2281 ggcgcttcca gagcgctccc agtaaaaaac gccgacattt ggctgacgtc aagtgtatac
     2341 ctttatactt taaaccgttt gacctgtgag tcaaggctct cagctagggt atcgagttct
     2401 tgggcagacg cgctgatctg ttgcgcgcct tgcgcctgtt gttcgatgcc ctgcgacatc
     2461 tgccgtatct gttccgagat ctcgtgggcg gattttgccg atgtgctcat cgctgaggcc
     2521 atctcctcgg cggctgcccc ttgctcctcg gaggtcgccg ttagattttc gatcatagca
     2581 gtcgtctttt ccaccatgtc cagcaaatgt ttgaattgct gttccaccgc ttgtgatttg
     2641 gtgttaacgt ctttcaccaa tccaacggtc tgacccgtcg cttgatagga ttcctcggcg
     2701 tctttttgca cttctttcaa aattgccccg atgttcgatg tcgctttttt actctcttcc
     2761 gctaattttc gaatctcgtc cgccaccacc gcgaaccctc gaccggcttc tcccgctcgg
     2821 gccgcttcta tcgcggcgtt aagcgctaac aggttcgttt gttccgcaat cgaagatatc
     2881 gtgtctacga tctcgcccac gctcttcgag ttctccgcta acttctggac cttttctgcc
     2941 gtcagcagcg tctgtttcgt tgtcgattcg atttctcgca cgacatccga gatcatctgg
     3001 ccaccttttc gagaaccgtc cgccgtcttg tcgttctcgg ttgagagcgc ctgcgccgtc
     3061 ttcgaaacgt tctgcgcgct cgccgccacc tcttccactc cggagtccac ctcttctatc
     3121 gctgctacgg tgttctgaag gttcgtgttc acgcttgccg attgaccgga taactcctcg
     3181 gatcccgcaa gttgttcctc cgagatcgcc gctaaatcag tcgaagcggc ttttatctct
     3241 ttcgaagccc ctcctattga gatcatcgcc gctctgaggt cgtgacccat cccgcccagg
     3301 gctttggcga tctgagcgat ttcatcgtgg cctttcgcct cgaaggccgt ggtcaaatcg
     3361 ccctttccaa agttatccac ctgaactgcg agttttttca tagatttgga gatgaccgtg
     3421 ccaacaaaca aggagatgaa cacaatgacg gcgacaatga tcacgaatac tgcgatgacg
     3481 attccgagga ggtatacgct cttctgattc gcctctgctt cggaaagggc gccggccacg
     3541 gaccaattag gagtattggg aatggggtcg tagtggagga tgtatttttc tcctgaaggg
     3601 aaaataatgt ttttaatacc gtcgctcccg cggatcatct cctttcctgc gtcttccaga
     3661 cccttgtacc ccgacttggt tccttccaac agattgagtt tcaagatctt atctgtgtcg
     3721 gggtgcgcga tcacagtccc cgctccatca accaggaaga tgtatccctc ttctccgatc
     3781 ttcgtggagg tcactttgtt actcaatatc ttaagagcca cggtggtccc aagcgcaccg
     3841 accacgtgtc cagtgtcgtc atagaccgca taggcagcgc caaaagcagg ggcattcgta
     3901 aacctgctga ttatgccgtc gttgagcgcg aattccttgc cccgaaacat aacgtcctgg
     3961 aaatagggcc gatcgctaat atccacgatt tcacccgttt ccattatagc ttttccggta
     4021 ggatcggcga gaaagaatgt ttcgaagagt cccccactct gccgtatagt ctcgatgaac
     4081 ctagggcaaa agcgggtcgg atcgaaagac ttgaccacat cagtattcga tagattccgc
     4141 atctccgttt taatcccagc cagcatctcc tcaacgatgt gcacgcaggc gtgcagagtt
     4201 tcttcagttg aatccgagga cagttccaag atcacacttc tcgcgttgag gccgataatg
     4261 aatccggcaa taacgagcac agaaaccgat acgggtatga agtacagtaa tagcttcgag
     4321 cgcacgcttt tgagtttcat catcatcttc tcctttcggt ttttcgaggg gattcttgcc
     4381 gtattttatc aagcgtcagc ctccacctct ttgagttatt aaacggaaac ctatctatta
     4441 taccatgatc gttatgggat tcaattattc ggggatcgct ttcgaaaatg agattggagt
     4501 gaaatagatt acggtatggt tgcctttaag actttgagtt agctgtattc gctgaatttt
     4561 tctttggcat ggctaaccgt attatatgag gagtaaaaaa gaaaagagtc tttcacacgt
     4621 agggcacggt ggaagttttt ctaggagtac ttagcagcag cgacttgcca gtctgcgtgt
     4681 tttttcgaaa aaggaaaact tgtcagacta aagttctgtg ttcccagaag tttggcatat
     4741 ccaggaatcc gtgtgggtca agctatgggg tgcaatcaag aagcaaggtc agatacgatc
     4801 acacgatttt ctttccacga agtcatcgcg gcgtaagcgc cccttcttct cgcgacggcg
     4861 cgatacgcct gggaacacct gggaacgcca aactttggtt tggcatggtt tgtgaaaata
     4921 gaaatgcagc agagcgtatc gcgaaggtat ga
//
LOCUS       DAOPXN010000170         4945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17105_length_4945_cov_5.776687, whole genome shotgun sequence.
ACCESSION   DAOPXN010000170 DAOPXN010000000
VERSION     DAOPXN010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17105_length_4945_cov_5.776687"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1525
                     /locus_tag="PKM49_08495"
     CDS             203..1525
                     /locus_tag="PKM49_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82870.1"
                     /translation="MWTFKTRAGGTTGKSIKRYDSSGDFKGEYDKLSEAVAEAVNGDR
                     IVVDGGMELKEEPEITIDGKEVTIRSSDPNNPFTIDMGGGRSAPGSKRGNNRVFHITN
                     GASVTIRGAIIKGGDATDDTLASGGGILIRGSSTVTTINATITDNKAKTGGGVSVILS
                     TFDTAIASNTAVIGPPVYGGGVAVLLGGTFNATETTIKSNTVIGETASGGGVTVLLYG
                     AFNAYEGTKITENKVEGEEDAYGGGVDVWQGTFNAYEGTEITENRVEGEENAYGGGVA
                     VTGDESGNGIFNAYGTKIADNTAEAKGDGAWAYGGGVAVGIFYAEEGIFNAYAGTTIT
                     GNTAEAEGESTASGGGVAVLPNGEFNAENVEISGNSAAVDGGGICWVCGEGGKIKTND
                     KEWDPPVEQNENFSVGTDGKIDSPPNPSHLVKVFDNDAVEGKQMSLWQ"
     gene            complement(2006..4312)
                     /locus_tag="PKM49_08500"
     CDS             complement(2006..4312)
                     /locus_tag="PKM49_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82871.1"
                     /translation="MKIALKILLLIIVVFAMFTIVAFVIKASNEETRAGASQVVESAE
                     TVSADFLLYAQAQEFDEAVGLLTREAFQIGYLQKENDVDSLKTRFDNQQALVIQKANV
                     LNSPALLQSINRIGDYAVELTKYKKDELRRLALLDQYHDYIENVLHPSCAANQAMVEG
                     FITIDDDRYAQAVQRIKELMDESMSAPEGERVQTFKGALKSEAVIEDLSLIEHETLWE
                     SDLLDERIRTLPFFKLMILRREVGLRDGDFYSLEESPNRLCNRLREELSALEAKGGFT
                     DAERMQKALVERSLLNYESTLSDYIDLLISTSFIEAEIALNNESIYKLQESIETVRSR
                     SLELVNKSINGEIEAINRLQTALYTEGQKRVETAISHMKEEGKRRVVATDLSGQRVLW
                     VVILAFAITALSFIYLYGLFRKLMKKLLPVAQRMKNLDFSGAMDYKPSKDEIGLVLGS
                     FKDVVDAIKTIAGEVAEAAHRIQLESETVVSSVEENSATAEEISSNMDDMNRGIERSV
                     KELGKVTEQTQSIALNSAETAKKVQKNVAETEKNLQDSDQNKKKIDGIVGRVEEVGTE
                     ISGTMKDILTLKNITEEIETFVEGISGIAEQTNLLALNAAIEAARAGDAGRGFAVVAD
                     EVRKLAVESNKMVEDIRRRIVVITQRVDQVVERSQTHVSGMEEVLTGIKVITQETNRI
                     TETLRTSQGLIRNFSDLLEGQNQEIGAVSKQSEKIADRFEEASRTIEGLNRSIKTTAD
                     SISELAQSAQALSEIAHQLDTRMKAFKI"
BASE COUNT         1096 a         1269 c         1371 g         1209 t
ORIGIN      
        1 cgccgggcgc tcaaacgaac gcgggcgaac gcgcggtgag tttatcgtac caggtctttc
       61 atagaaaaca gggagaggaa taccccaacg cacccgccga aacgacttat aaattcttag
      121 cttgaccgac ctcgaccccg acgccacata cacgtggtat gtgaaggcgg tacaatcgga
      181 caatcagaca gagacaagcg acgtgtggac gttcaagacg cgtgcaggag ggaccacagg
      241 aaaatctatc aagcgatatg actcttcagg agactttaaa ggagaatatg acaaactcag
      301 cgaggcggtc gccgaagcgg taaatggaga ccgcatcgta gtggacggcg gaatggaatt
      361 gaaagaagag cctgaaatca ccatcgatgg gaaagaggtc acgatccgct cttcggatcc
      421 aaacaatccc tttactatag atatgggtgg tgggagaagc gccccaggtt ccaaacgagg
      481 gaacaaccga gttttccaca tcactaacgg tgcttcggtg accatccgcg gcgcgatcat
      541 caagggcggt gatgcgaccg atgatactct agctagcggc ggcgggatac tcatcagagg
      601 aagtagtacc gtcacgacca tcaacgcgac gattactgat aataaagcaa aaacgggtgg
      661 cggagtgagc gtgattctta gcactttcga cacggcgata gcgtcgaaca ccgcggttat
      721 tggacctccc gtctatggcg gcggcgtggc tgtcctctta ggcggcacct tcaacgccac
      781 ggaaacaacg attaagagca acaccgtaat aggcgaaacg gcatctggcg gcggcgtgac
      841 tgtcctcctt tacggcgcat tcaatgctta cgaaggcacg aagatcacgg agaacaaagt
      901 ggaaggcgaa gaagatgcct atggcggcgg cgtggatgtc tggcaaggta ccttcaacgc
      961 ttacgaaggc acggagatca cggagaacag agtggaaggc gaagaaaatg cctatggcgg
     1021 cggcgtggct gtcacgggcg atgaatcggg aaacggaatc tttaatgctt atggcacgaa
     1081 gattgcggat aacactgcgg aagcgaaagg cgatggtgcc tgggcctatg gcggtggcgt
     1141 ggctgtcggg atcttttatg cagaagaagg aatcttcaac gcttacgcag gcacgacgat
     1201 cacggggaac accgcggaag cggaaggcga atcaacggct tctggcggcg gcgtggctgt
     1261 cttgccgaac ggcgaattca acgctgaaaa cgtcgagatt agtgggaatt ctgcagcagt
     1321 ggatggcggt gggatttgtt gggtctgtgg ggaaggaggt aagattaaaa cgaacgataa
     1381 agaatgggat cctcctgtag agcaaaatga aaatttctcg gttggtacgg atggaaaaat
     1441 agactcgcct ccaaatccca gccaccttgt taaagtcttt gataatgatg cggtcgaagg
     1501 caaacagatg agcctctggc aataaaaacg attgaagtgc cttggaggag ccgcactttt
     1561 gtgcggctcc ttttttttga agagccttgt cccttgccag ttttttctcc acgccctctg
     1621 tgcgaacccc tgtttttaat ctttggaacg aacacctggg tatcgcgccg tcgcgaaaag
     1681 aaggggcgct tacgccgcga tgcccaagtg gcgttatcgc ggggtaacta acaggacccc
     1741 gcgatccgct cttcttttct aaaacatgcc aacttaaaag ttggcgttcc caagaacctt
     1801 ttctccgcgt gtctcctgtt ttcctttgtg ttctccgtgc cctctgtgcg agcccttttt
     1861 tggtttttga aagacatgcc aaactgaagt ttggcgttcc taggcgttct caagcgtatc
     1921 gcgccgtcgc aaagagaagg agcgcttacg ccgcgatgcg cggcgttccc aggtatgcgg
     1981 tattcccagg gttcccagcg tttctctata ttttaaaggc tttcatgcgg gtgtcgagtt
     2041 ggtgcgcgat ttcagacaag gcttgggcgg attgcgcgag ttcgctgatc gagtcggcgg
     2101 tggtctttat ggatcggttg agtccttcga tcgttcgcga ggcttcttca aaacggtcgg
     2161 cgatcttttc ggattgcttg gagaccgcgc cgatctcttg gttctgaccc tccaataggt
     2221 ccgagaagtt tcttatcaat ccttggctgg ttcggagcgt ttcggtgatc cggttcgttt
     2281 cttgtgtgat gaccttgatt ccggtcaaca cctcttccat cccgctcacg tgggtttgtg
     2341 accgttcgac aacctgatcc actcgctgag tgatgacgac gatacgtcgg cgtatgtctt
     2401 ccaccatctt gttggattcg acggctaatt tgcgtacttc gtcggccacg acggcgaaac
     2461 cccttcccgc gtcgcccgcg cgcgcggctt cgatggcggc gttgagcgcg agtaggttcg
     2521 tctgctccgc aatcccggag atgccctcca cgaaggtttc tatctcttcg gtgatgtttt
     2581 tcagcgtgag gatgtctttc atcgttccgg atatctccgt acctacctct tcgacccggc
     2641 ctacgattcc gtcgatcttt ttcttgttct gatcggagtc ttgtaggttc ttttctgtct
     2701 cggcaacgtt tttctgtacc ttcttcgcag tttcggcgct gttcaaagcg atactctgcg
     2761 tttgctcggt gaccttcccc aactctttca ccgatcgctc gatgccacgg ttcatatcgt
     2821 ccatattcga ggagatttct tccgccgtcg ccgagttttc ctcgactgat gagactaccg
     2881 tttcggattc gagctgtatc ctatgggccg cctctgcgac ctcgccggcg atggttttta
     2941 tggcgtcgac cacgtctttg aagctgccca gaaccaagcc gatctcatct ttggagggct
     3001 tgtaatccat cgcgccggag aagtcgaggt ttttcatcct ctgggcgacc ggaagcagtt
     3061 tcttcatcag cttccggaat agtccgtaga ggtatatgaa tgagagcgcg gtgatcgcga
     3121 aggccaatat gaccacccac aacacccttt gtcctgagag gtcggtagcg accaccctgc
     3181 gcttcccttc ttctttcata tgggagatcg ccgtttctac cctcttttgc ccttccgtgt
     3241 acaaagcggt ctgcaatcgg ttgatcgctt cgatctcgcc gttgatgctc ttgttcacga
     3301 gctccaaaga ccgagagcga accgtttcga tgctttcttg gagcttgtag atcgattcgt
     3361 tgttgagggc gatctcggct tcgatgaagc tggtggagat gagcaaatcg atgtagtcgg
     3421 atagtgtgga ttcgtagttc agcagactcc gttcgaccaa agccttttgc attctttcgg
     3481 catcggtgaa cccgcctttc gcttccaagg cggagagctc ttctcgtaac cggttgcaaa
     3541 gtcgattggg actttcttct agggagtaga agtctccatc cctcagccct acctctctgc
     3601 gcaagatcat gagcttgaaa aaaggaaggg tccgtatccg ttcgtcgagg aggtcggatt
     3661 cccagagggt ttcgtgttcg atcaagctga ggtcttctat cacggcctcg gatttcagcg
     3721 cgcctttgaa ggtctgtaca cgctcgccct cgggggctga catcgattcg tccataagct
     3781 ctttgatccg ctgaacggct tgggcgtacc ggtcgtcgtc gatcgtgatg aagccctcca
     3841 ccatcgcctg gttggctgcg caagagggat gcaacacgtt ctcgatgtaa tcgtggtatt
     3901 gatccagcag cgccaatctc cgcagctcgt cctttttgta tttcgtcagc tccaccgcgt
     3961 agtccccgat tctattgatg gactggagca aggcgggtga gttcagcacg ttcgctttct
     4021 ggatgacgag cgcctgctgg ttgtcaaatc gtgttttcaa tgagtccacg tcattttctt
     4081 tttggaggta tccaatctga aaagcttctc gggtgaggag gccgaccgct tcgtcgaatt
     4141 cctgcgcttg cgcgtacaga aggaaatcgg cgctaaccgt ttcggcgctt tctaccactt
     4201 ggctcgcgcc tgcacgggtc tcttcattag aggctttgat aacgaacgcc acgatcgtaa
     4261 acatcgcaaa aaccacaatg atgagcagca gtattttcaa tgcgattttc atgagaccac
     4321 cctctcgatt ttctttggat gactcaatta tacgcgaaac ggttgcgaat ttccatagtt
     4381 attttacgtt aacggtagaa ggtgtgggta tgatatcgag gcgcaaaaaa ggaaagaaaa
     4441 agaaggggcg cttacgcgga gatggtctgt gtaagttatc gcggggtaac cagcgggctg
     4501 acttcggaag cccacgaagt ctcgcgatcc gccttttgcc tttccaaaac caaaagaagt
     4561 ttcgcacaga gagcacagag tacacagaga gaacacagag tacacagaga gaacacagag
     4621 aaatgccctg agacgccgaa tttcaaaggt catcgcgggg taaccaacgg gaccccgcga
     4681 tacatccttc gtctttctca agagggtatc gcgccgtcgc gaaaaaaagg gcgcttacgc
     4741 cgcgatgccc gccgtgtggg gttatcgcgg ggtaaccaac gggaccccgc gatacgcctt
     4801 ttgtttttta acctgtcgcg aacggcacat aaaaaaaggg tatcgcgccg tcgcgaagac
     4861 ataaaccgct tacgccgcga tgcccgccgt gtggggttat cgcggggtaa ccagcgggct
     4921 gacttcggaa gcccacgaag tctcg
//
LOCUS       DAOPXN010000171         4910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17271_length_4910_cov_5.335118, whole genome shotgun sequence.
ACCESSION   DAOPXN010000171 DAOPXN010000000
VERSION     DAOPXN010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17271_length_4910_cov_5.335118"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            971..2788
                     /locus_tag="PKM49_08505"
     CDS             971..2788
                     /locus_tag="PKM49_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNY82872.1"
                     /translation="MKGMVVSTWIETWKKLFGEKNVVETAKSANFPVDRVYSPLEDVD
                     ENVLIAFSAKLAKKVNLTENDLWKKTGLENIRTFHKWYPMYFKKASFLSFLAAMDLVH
                     RLLTRRIKGAKPPRLFFELVDPTRARLRYVSFRDLRSYFLGLLQGASTYFKENISVEI
                     IEQGKKDGKTYIEVEVTAQTPYSENPTFPFLKVFSLGFIKSFFVISVFLIPAIVGVLA
                     WLFFTRFSQPWLAALLTGAATLALSYFPLRDLKKGLGSIKKGLKFLAAKELDKPVIIK
                     DPVELAEMSKAYTEASHTLKETMTGFMGDVQEIETFTEKISGSVEEISGLIDTMSDLS
                     HQVATSAVDISEDTESISSAVNSNVSVLRDIVDRETEMVASLNKAVQDIVDASRNVED
                     SSNGIGKMSEDFAKLMTVGKDLQSQAGMIMNIAETVTSIAGQTNLLALNAAIEAARSG
                     EAGRGFAVVAEEIRKLAEESGTSAEQIAENLGIINTGINRLTDGLSSQFEEMKTQSGN
                     LKANSERNRDSTTRISSVSQEINHLIVNLQEEAKKLEHISSSIENLLAISEESSATAE
                     EISASTQKFLDEVKEILTSVGKISEFIGSLNENLRGIKL"
     gene            complement(2832..4634)
                     /locus_tag="PKM49_08510"
     CDS             complement(2832..4634)
                     /locus_tag="PKM49_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNY82873.1"
                     /translation="MVRKSFRRLVFWVSALILAGIAVTIGFFFFYSNERRSFRVTANE
                     IRATNQTLNQMIAKIHQLNLLKTSWDQQPAIEYTETDDFKAIYELYKKDFDTVSGFLE
                     KNRRSTNRVSIENYPYALFEGREATVIVDEIGDLIRSLNKWREELEEKIESLPINLFV
                     VNLIAVFLSFISLFILSKLFFGFSRRIETAFAHFGALLDGNPNVEVYRPVWKEENRIH
                     QTIVQLEQRIRFDRELIETQNLQTLEDLIPNLFPIIRERIGADRIAFAFVDNLENVIA
                     ETAVSLSGKIKLKAGFAVNIHRTTLDKLGCESPPRIIYDLEYHYAHVHQSEPTRLLLE
                     EGVRSSITFPICLNNRILGFFFVSSFSKAAFRIMDAEFLKEIVSSLKYTILNSYILQQ
                     VIAVTAQTFADLVEKKDFETGNHIVRVSRLARMLAEDLTETFPEITTKFIREIFWFAS
                     LHDIGKVGIPDNILLKPAKLTPEEFEIMKTHVTIGESVIRQMQRTLHRFTHLEFLSVA
                     IDAIIGHHEKWNGSGYPAGMKGDEIPLAGRILAVSDVFDALMSERPYKKAFSFEKSIE
                     ILSEGKGAHFDPRIVDAFFKRKEEVKVLYDKFKD"
BASE COUNT         1296 a         1176 c         1266 g         1172 t
ORIGIN      
        1 tttccgcctg tatcgtgccg acatcgatag tctgtaagac ttcaggggga agataggtct
       61 ctaagaacgc ttgggtattt gcgacattcc ccagcacttc tttgaaaaac ctatcgtggg
      121 ggttataaaa cttctccatt attgtcctcc ctacgggaaa tcatttcaca ggtattatac
      181 actaataaag agtgggagtt tggttttccc gagagtgaaa aacgagaaca gaaggtttcg
      241 cacagagggc acggagaacg cggagagaac gtagatcgta gcataagcgc cctttctttt
      301 cgagacggcg cggcacgctt ttattcgatc tctggccccg ttttcgctac ttgctaatgt
      361 acacttactg ctaaagatag gatcgcgaga cttcgtgggt ttccgaagtc agcccgctgg
      421 ttaccccgcg atgagcctaa aagtcaacgt ggcgaaaacg attatgactt cacagaacac
      481 ccccagaaag gtgttttcag aaaggagttc caagaacctt cctgagccgc ccccagcgcg
      541 aacctacttc cgtgttctct gtgccctctg tgtgaaccct gttttattct taagatatgc
      601 caatttaaaa gttggcgttc ccaggcattc ccagacgctc ccaagcgcat cgcggcgtag
      661 caggaagggg ctaagggcgg atcgcggggt cgtaaaaaac cacttacccc gcgataacac
      721 gcacggtcat tgcggcgtct taaaaaaaca aaattcgtat cgcgagactt cgtgggcttc
      781 cgaagtcagc ccgctggtta ccccgcgatg aaccttttag ttatgtcacc tcataacttg
      841 tcccacacct attttggaac acttcgaaaa actgtggtaa aatcgaatcg ttcgaaatag
      901 agaagaaaaa actccggttc aaaattgcgc gctggcttac aagctctcgt aggaataagg
      961 aggagcattt atgaaaggaa tggtcgtcag cacttggatt gaaacttgga aaaagctctt
     1021 tggagagaag aacgttgtgg aaacggcgaa aagcgcgaat tttcccgtcg atcgcgttta
     1081 ctcccctttg gaagatgtcg atgaaaacgt cctcatcgcc ttttcagcaa aattagcgaa
     1141 aaaggtgaac ctcaccgaga atgatctttg gaaaaaaaca ggcttggaga acatccgcac
     1201 cttccacaaa tggtatccga tgtacttcaa aaaagccagc ttcctctcct ttttggcggc
     1261 aatggacttg gtgcatcggc tattgacaag acggatcaaa ggcgcgaaac ctccgaggct
     1321 gttttttgaa ctagtagatc ccacaagggc taggctccgt tatgtgtctt tcagggacct
     1381 caggagctat ttcttaggac tcttgcaagg cgcctcaacg tatttcaaag aaaatatctc
     1441 cgtcgaaatc atcgaacagg ggaagaagga tggaaagacc tatatagaag tcgaagtcac
     1501 cgcgcaaaca ccgtactcag aaaacccgac cttcccgttc ttgaaagtgt tttcgctcgg
     1561 attcatcaag tctttcttcg ttatctcggt ctttctgatt ccggcgatcg tgggagtcct
     1621 cgcttggctt ttcttcacgc gcttttctca accgtggctc gccgccctgc taaccggagc
     1681 cgccaccctc gcgttaagct atttcccgtt gagggacttg aaaaaggggc ttgggagtat
     1741 aaagaagggg ttgaaattct tagccgccaa agagcttgac aaaccggtca tcatcaaaga
     1801 ccccgtcgaa ttggccgaga tgtcgaaggc gtacactgag gcgtctcata ccctgaaaga
     1861 gacgatgact gggtttatgg gagacgttca ggaaatcgag accttcaccg aaaagatctc
     1921 aggctcagta gaagagataa gcggcttaat cgatacgatg agcgacctct cacatcaagt
     1981 ggcgacttct gccgttgaca tttcggaaga taccgagtcg atctcctcag ccgtaaattc
     2041 caacgtttcg gttttaaggg atatcgtaga ccgggagacg gaaatggtcg cttctttgaa
     2101 caaagccgtt caggacattg tcgacgcctc gcgaaacgta gaagactcct caaatgggat
     2161 aggcaagatg agcgaagact tcgcgaaact gatgacagtc gggaaagact tgcaatcgca
     2221 ggcgggaatg attatgaaca ttgcggaaac ggtcaccagt atcgcgggtc agacgaactt
     2281 actggcgttg aacgccgcga tcgaagccgc gcgaagcggt gaagcgggaa ggggattcgc
     2341 ggtcgtggca gaagagatca ggaaattggc cgaggaaagc gggacttccg ccgaacagat
     2401 cgccgagaac ctcggtatca tcaacacggg gatcaatcgc ctcactgacg ggctgtcctc
     2461 gcagtttgaa gagatgaaaa cgcaatccgg gaacttgaaa gcgaattccg aacggaatcg
     2521 tgactcgacc acccggatct cgtcagtctc tcaagagatc aaccacctca ttgtcaattt
     2581 gcaggaagag gccaaaaagc tcgaacatat ctcgagcagt atcgaaaact tgttagcgat
     2641 ctccgaagag agctcggcaa cggccgagga gatcagcgct tctactcaaa aattcttgga
     2701 cgaggtcaaa gagatcctga caagtgtcgg caagatcagc gaattcattg gcagcctcaa
     2761 cgagaaccta agaggaataa aactctgatt gagcatcgta gggggttgaa aaggttcgac
     2821 aggatacgcc gctagtcctt aaacttgtcg taaagaacct tgacctcttc cttcctctta
     2881 aaaaaagcgt cgacgatgcg cgggtcgaaa tgagcgcctt ttccttcgga taagatctcg
     2941 atggattttt cgaaggagaa ggccttttta taaggacgct cgctcatcaa ggcgtcgaac
     3001 acgtccgaga cagcgagaat cctacctgcc agcggtatct catccccttt catccccgcg
     3061 gggtaaccag agccgttcca tttttcgtgg tgcccaatga tcgcgtcgat ggcgacgctg
     3121 agaaactcta aatgggtgaa gcgatggagg gtgcgttgca tctgccgtat aacggactcc
     3181 ccaatcgtta cgtgggtttt cataatttcg aattcttccg gagtcaattt cgccggtttg
     3241 agcaagatat tatcgggaat tccaaccttt ccaatatcgt gaagggaggc gaaccaaaat
     3301 atctctcgta tgaacttcgt ggtgatttcg gggaaggttt cggttaagtc ctccgccaac
     3361 atacgggcca atcgggacac cctcacgata tggttcccgg tctcgaaatc cttcttttcc
     3421 accaagtctg cgaaggtttg agctgtcacg gcgatgacct gttggaggat gtaagagttc
     3481 aagatcgtgt atttgagaga ggatacgatt tctttgagaa attccgcatc catgattctg
     3541 aacgcggctt tcgaaaagct gcttacgaag aagaacccca aaatgcgatt gttcaaacag
     3601 atgggaaagg ttatgctgga gcgcaccccc tcttccagca gcaaccgtgt gggttccgat
     3661 tgatggacgt gcgcgtagtg gtattcaaga tcatagatga tccgcggcgg tgactcgcag
     3721 ccgagcttat cgagtgtcgt gcggtggatg tttacggcaa aaccggcttt caacttgatc
     3781 ttcccggata ggctgacggc ggtctctgca atgacgttct caaggttgtc gacgaaggcg
     3841 aaggcgatcc gatcagcgcc gattcgctcc ctaatgatcg ggaacaagtt tgggattagg
     3901 tcttccaagg tttggaggtt ttgggtctcg attaattccc gatcgaatcg gatacgctgt
     3961 tcgagctgga cgatcgtttg gtgaatccgg ttctcctcct tccaaaccgg gcgataaacc
     4021 tcgacgttag ggttaccgtc caagagcgct ccaaagtggg cgaaagctgt ttcgattcgc
     4081 cgggagaacc cgaaaaagag cttgctgagg atgaatagag agataaagct gaggaatacg
     4141 gcgatcaggt tgaccacaaa caggttgatc ggaaggcttt cgatcttttc ctctagctcc
     4201 tcccgccatt tatttaggga acggatcaaa tcgccgattt cgtcgacgat gacggtcgct
     4261 tctcgaccct cgaacaaagc gtacgggtaa ttttcgatgc tcacacgatt cgtagaccga
     4321 cggttttttt ccaaaaagcc gctgacggtg tcaaaatcct tcttataaag ctcgtagatg
     4381 gctttgaaat cgtcggtctc ggtatactcg atcgcgggtt gctgatccca actggtcttt
     4441 aacaggttca actgatggat tttggcgatc atttggttca gggtttggtt ggtcgcgcgt
     4501 atctcattcg cggtaacccg gaaagaccga cgttcgttgg aatagaaaaa gaaaaaacca
     4561 atcgtgaccg cgattccggc gaggatgagg gcgcttaccc aaaagacgag tcttcgaaaa
     4621 ctttttctca ccataagaac gcctccaaaa aacaaggttc gatataagga aaacgaacgc
     4681 cctgcttact cgaagattgg ggggcgagtt agaattatat cacaaaaaca cctcgacaag
     4741 cttcagaagt gtaggtataa tatcagggtt ccccaaaatg ggagcgctga actttagttc
     4801 gacacgtttt tgcttttaca gcgtatcgcg ccttcgcaaa aagaaggagc gcttacgccg
     4861 cgatgcccga acggggttat cgcggggtaa ccagcgggct gacttcggaa
//
LOCUS       DAOPXN010000172         4868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17486_length_4868_cov_5.954290, whole genome shotgun sequence.
ACCESSION   DAOPXN010000172 DAOPXN010000000
VERSION     DAOPXN010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17486_length_4868_cov_5.954290"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PKM49_08515"
     CDS             <1..1262
                     /locus_tag="PKM49_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNY82874.1"
                     /translation="ILAVHPPVDGQNVRTSIDLTLQQILSKRLHEGVTTYGAKSATGV
                     LIETKTGKVRALYSTSGLNEAVISVFEPGSALKPFIFASAMKYNLLDDDETFFCTGKI
                     KPFPDLSTIINDTHVHHEIQTPDALAHSCNVATIQIALRFLEEMGDWTWYRELRLAGF
                     GDLTGVEYPGEVKGILHNPATWNRLTGIQMSIGQGIAVTALQLTASFNIFANNGVYIS
                     PTLLEDRVNPRNERRVYPESVVKRIVAMLHKTVTSGTATGARSRLTDSAGKTGTAQKA
                     YAGKGYVKGKYVSSFIGFFPVEDPMYTMLISLDEPQGDIYYGGDISAPIFKNVVEDYL
                     TILETAASLKTEPIVFKSWKMPDFRGLTRKDAIDILAKLEIPPQKVRFEGEGVIISQS
                     PAEETPIHTVDEIVLTLGRRSDSYGNP"
     gene            1249..3477
                     /locus_tag="PKM49_08520"
     CDS             1249..3477
                     /locus_tag="PKM49_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNY82875.1"
                     /translation="MEILDSKTAGPAIAPSIGKALFKDMSWRGLRPLQENSGNAIRNG
                     YQTLITAGTASGKTEAALLPVLSLILESPDLAHSRTPVALYVAPLKALINDIAERLKK
                     LSLRAPLEIYAWHGDVEMSEKNEALKHASILVTTPESLEGLLVSSKIDPTLFFHNLRF
                     FLLDEFHALLSSPRGYQLASQIERLQTYSRYEIQKIAMSATIGNPEMVLEWMRGSGTR
                     ECLLVSDSHQTQKQLMLRKKSYETLLADAQKVVSLGKKAILFTCSKGEAESLFHFLNR
                     KGISVLLHHGSIGKKQREDTERLFKGSDDYPIMIATTTLEMGIDIGDVSYVLFYSVPR
                     SAASFMQRLGRAGRKTGVARALIYVPTDTEEEGKPLALDQTLEERLFLLGNLSLFSEG
                     RVEPIEMKDYYPQLFAHQVISMVFREKQMDRRKIALLKAAYPFKGLQKEDVGAVLSFL
                     EEENYLLRIDQEYRLGWKAKDRLEGAGIGDFLSVFSSSEDWTVVEGNREIGKVHAATV
                     FHNKRKQGHEVFIQNFYLAGKAWKVLSVDAARRRISVQRAESGAKPLWLSGEDAVHPV
                     FSEAIRRFILKPTFSPSCVWDPEVQDTLLKTIAGELYTVSHEERWVPVMVERKKGADI
                     HLYTYAGTLLNDLLALLVRQYAPQSRDVRSTWRVIRWKAYAPFHPEEFKDWITSLSYE
                     EFYGLTEDEFTRNEPKFYGKVFPDCLREYIPASLSVNALIRHYWGLLQSSVRSGGLTS
                     DA"
     gene            3514..4215
                     /locus_tag="PKM49_08525"
     CDS             3514..4215
                     /locus_tag="PKM49_08525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNY82876.1"
                     /translation="MSRFYYAVKVEENKAWFDDHEYRHFQVMRVEEGQRIVFTDGNGA
                     LYAGIVSFARDRTTARITEKISQIERSPSQRVAVGIAHAQWDREEVLIEKGTELGVTD
                     FYLFPSRYSKYRDINLNKVSYTLRKAIKQSENPLLPTFEKFGRLEEALALVERYPQRI
                     LLQPSSPTDLFDTRRQSGPALLFVGPEGGFHPDEVQLCIRAGFTPCSIGKRILRLETA
                     ALCGFVWATLLSSVD"
     gene            complement(4300..>4868)
                     /locus_tag="PKM49_08530"
     CDS             complement(4300..>4868)
                     /locus_tag="PKM49_08530"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNY82877.1"
                     /translation="TGGISRRMNHVFKGALEIDGLSFVERLIKKLSPIFEETVLAGSQ
                     KELEQYENDPRLTVAPDRYLGIGPFAGILTAFETTGAEELFLCPCDSPFVTVEIVREL
                     LAVRWGFNADITIPISGNRFYPLIGLYHRRVVPRIHELVEGGRNAIRFLFRTCPTLMV
                     HFKDPTPFLNINTWEDYERLLKNAKPLD"
BASE COUNT         1115 a         1318 c         1424 g         1011 t
ORIGIN      
        1 agatactcgc ggtccatccg ccggtggacg gccaaaatgt caggacctcg atcgatctga
       61 ccttacagca aattctgtca aagcgacttc acgaaggggt cacgacctac ggagcgaaat
      121 ccgcgacggg tgttttgatt gagacgaaga ccggcaaagt gcgcgcctta tacagcacga
      181 gcggcttgaa cgaggcggtc atctcggtct ttgagcccgg gtccgctttg aaaccgttca
      241 tattcgcttc ggcgatgaaa tacaaccttt tggacgacga cgagaccttc ttctgtacgg
      301 gaaagatcaa gcctttcccc gatctgtcga cgatcatcaa cgacacgcac gtccatcacg
      361 agatacagac tcccgacgct ttggcccatt cgtgcaacgt cgcgacgatc cagatcgcgt
      421 tacggttttt ggaagagatg ggcgactgga catggtaccg agagctgcga ttggcggggt
      481 tcggtgactt gacaggtgtg gaatacccgg gtgaggtcaa aggtattctt cacaacccgg
      541 ctacttggaa ccgcttgacc ggcattcaga tgagcatcgg gcaggggatt gcggtgaccg
      601 cgttgcagtt aaccgccagt ttcaacattt tcgcgaacaa cggggtctat atctcgccta
      661 cccttctgga agatcgggtc aacccacgaa acgagcgcag ggtctacccg gaatcggttg
      721 tcaagcgcat cgtcgctatg ctgcataaga cggtcacatc aggaaccgcc acgggcgccc
      781 gctctcggtt gaccgacagc gctgggaaga cgggcaccgc acagaaggcc tacgcgggta
      841 aagggtacgt caaaggcaag tacgtctcga gcttcatcgg tttcttcccc gtggaagacc
      901 cgatgtacac gatgctcatc agcctggacg agccgcaggg cgatatctac tatggagggg
      961 atatctccgc gccgatcttc aagaacgtgg tggaagacta cctgacgata ctggaaaccg
     1021 ccgcaagtct caagaccgag ccgatcgtct tcaagtcctg gaaaatgccg gacttcaggg
     1081 gtctcacacg aaaagacgca atcgatatct tggcgaagtt ggagataccg ccccagaagg
     1141 tgcgatttga gggtgaaggg gtgatcattt cccagagccc cgctgaagaa actcccatac
     1201 acacggtcga tgagatcgta ctcacgctcg gaaggaggtc cgattcctat ggaaatcctt
     1261 gattcgaaaa ccgcggggcc cgcgatcgcg ccctcgattg ggaaggcgct cttcaaggat
     1321 atgtcctggc gcgggctgcg accgcttcag gagaacagcg ggaacgcgat cagaaacggt
     1381 tatcagactt tgatcactgc gggaacggct tcgggaaaga cagaggctgc gctgttaccg
     1441 gtcctgtcgc tcatcttgga gtcgccggac ctcgcgcaca gtcggactcc cgtcgccttg
     1501 tacgtggctc cactcaaagc cttgatcaac gatatcgccg aaaggctcaa gaaactgtcg
     1561 ctccgcgccc cgctggaaat ctacgcttgg cacggtgacg tggaaatgtc cgaaaaaaac
     1621 gaggcgctga aacacgcgtc gattctcgtg accaccccgg aatcgctgga ggggttgctc
     1681 gtctcatcga agatcgatcc gacgcttttc tttcataacc tccgcttctt tctcctggac
     1741 gagttccacg ctctactgtc ctcaccgagg ggctatcaac tcgcctcgca gatcgaaaga
     1801 ctccaaacct acagtcgata cgagatacag aagatcgcga tgagtgccac catcgggaac
     1861 cctgaaatgg tcttggagtg gatgcgcgga agtggtaccc gggagtgcct cctcgtgagc
     1921 gactcacacc aaacgcagaa gcagctgatg ctccgaaaga agtcctacga aacccttctg
     1981 gccgacgcgc aaaaagtggt gagcctgggg aaaaaggcca tccttttcac ctgcagcaaa
     2041 ggagaggcag agtcgctctt ccatttccta aacaggaaag gaatctccgt tcttctccac
     2101 cacggttcga tagggaaaaa gcagcgggag gataccgaac ggcttttcaa gggctcggac
     2161 gattacccga ttatgatcgc cacgacgact ctcgagatgg gaatcgatat cggcgacgtc
     2221 tcatacgttt tattctatag cgttccgcgc agcgcggcgt cgtttatgca acgtttaggc
     2281 cgcgcgggcc gaaagacggg ggtcgcgcgc gcgctcatct acgtgccgac ggataccgag
     2341 gaggaaggga agccgctggc tttggatcaa acgctggagg agcggttgtt cctgttgggc
     2401 aacctttccc tgttttcgga agggcgagtg gagccgatcg agatgaaaga ctactacccg
     2461 cagcttttcg cgcatcaggt catttcgatg gtctttcgcg agaaacagat ggaccggcgg
     2521 aagatcgccc ttctcaaggc ggcctatccg tttaaaggcc ttcagaaaga agatgtgggc
     2581 gccgtgctct cctttctgga agaggagaac tacctccttc ggattgatca ggagtatcga
     2641 ttggggtgga aggcaaagga ccggttggag ggggccggga tcggtgattt tttaagcgtt
     2701 ttcagctcat cggaagactg gaccgttgtg gaagggaacc gcgaaatcgg caaggtgcac
     2761 gcggccacgg tctttcacaa taaacggaaa cagggccacg aagtcttcat acaaaacttt
     2821 tacctcgctg gaaaggcctg gaaggtctta tccgtagacg cggccaggcg ccggataagc
     2881 gttcaacgag ccgaaagcgg agccaagcca ctgtggctaa gcggagaaga tgcagttcat
     2941 cccgtttttt ctgaagcgat acgccggttc atcctgaagc cgaccttttc tccttcttgc
     3001 gtttgggatc cggaggtcca ggatacgctc ctgaaaacca tcgcggggga actgtacacg
     3061 gtgagtcacg aagagcggtg ggttccggtg atggtagaac gcaagaaggg cgcggatatc
     3121 cacctctaca cctacgcggg cactctgttg aacgatttgc tggcccttct ggtgagacag
     3181 tacgcgccac aatcgaggga cgtccgatca acctggcgtg tgatccgatg gaaagcctac
     3241 gccccgttcc acccggaaga attcaaggat tggatcacgt ctttatccta tgaggagttt
     3301 tacgggctca ccgaagacga gttcacgcga aacgagccca agttctatgg gaaggttttc
     3361 cccgattgcc tcagggaata catcccggcg agcctgtcgg tcaacgcgtt gatccgtcac
     3421 tattggggcc tgctgcagtc gagtgtccgt tccgggggcc tcacgtcgga cgcgtaggca
     3481 gagactcgcc tcctaagcgg aaagggggcg catatgagtc ggttttacta cgcggtgaag
     3541 gtggaagaaa acaaagcctg gtttgacgac cacgagtacc ggcacttcca ggtgatgcgg
     3601 gtggaagagg gacaacggat cgtgttcacg gacggcaacg gcgccttgta cgccggtatc
     3661 gtgagcttcg cgcgcgaccg aacgaccgcc cgcataacgg aaaagatcag ccagatcgag
     3721 cgctcgccct ctcaacgggt ggccgtcggt atcgctcacg cccagtggga tcgggaagag
     3781 gtcttgatcg aaaagggaac ggagctgggg gtgacggact tctacctctt tcccagccgt
     3841 tattcgaagt atcgggatat caacctcaac aaagtttcct ataccttgag aaaggcgatt
     3901 aaacaatccg aaaacccgct tctgccaact tttgaaaagt tcgggaggct cgaagaggcg
     3961 ctcgccctcg tcgagcgata cccgcagagg atcctccttc agccgtccag cccgaccgat
     4021 ctgtttgata cccggcgaca atcgggcccg gccctcctct tcgtggggcc ggaaggggga
     4081 ttccacccgg atgaggtgca actctgcatc cgcgcgggct tcacgccctg ctcgatcgga
     4141 aaacggatac tccgactcga aacggctgcg ctctgcggtt tcgtgtgggc cactctcctg
     4201 tcgtccgttg attgaagcgg gcggtccgcc cattttctta tggaaaaagc atgaccgact
     4261 ccgggcggct ttaccgggtc tatgtcatct aaagctcctt taatcgaggg gtttcgcgtt
     4321 tttcagtaaa cgttcgtagt cttcccaggt gttgatgttt aagaaaggcg tggggtcttt
     4381 gaaatggacc atcagcgtcg ggcaggttct gaagagaaag cggatcgcgt tacgacctcc
     4441 ttcgacgagc tcgtggatac gagggacgac gcggcggtgg tagagaccga tcagcgggta
     4501 aaaccggttt ccggaaatcg ggatggtgat gtcggcgtta aagccccacc gaactgccaa
     4561 caattcacga acgatttcga ccgtgacaaa agggctatca cacgggcaca aaaagagttc
     4621 ttccgcgccg gttgtctcga aggcggtgag tatgcccgcg aacgggccga tcccgaggta
     4681 tcggtcgggc gcgaccgtta ggcgtgggtc gttctcatac tgttcgagct ctttttgaga
     4741 gccggccaac accgtttctt caaagatagg gcttagtttt tttatcagcc gttcgacgaa
     4801 agagaggccg tcgatctcga gagcgccttt aaacacgtgg ttcatacgac gggaaatccc
     4861 ccccgtca
//
LOCUS       DAOPXN010000173         4850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17551_length_4850_cov_5.141397, whole genome shotgun sequence.
ACCESSION   DAOPXN010000173 DAOPXN010000000
VERSION     DAOPXN010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17551_length_4850_cov_5.141397"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4370)
                     /locus_tag="PKM49_08535"
     CDS             complement(<1..4370)
                     /locus_tag="PKM49_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HNY82878.1"
                     /translation="MKNAVRLFLLLIAISSVAFGYISADRNAILYPYQSYSFWAYNEP
                     DGVTVNLYYIGPTLQDFLTEFKRNTLKKPPKQADHAVYFEEAKAWKRLEIPSGYFERV
                     GTYVLEYVSKDRKDYTLLLKTNVNGALVSIGESVYIKLWDNIEGKDVRFTQILTGYTS
                     PKPLSIAPSAGLVEIELSKIVEETLFVETPNGILFLSDALRQADERLDPLIFLTDRPL
                     YRPGDLVQARAIFLNSKTGELLTNRPIEVAIVDPLGREKMKGVFHSDEWGGISFIYET
                     FPDDTRGHYTVKIRYGDDVSYRYFELADYAKPSFTVNASLQKPVYALDEPIELRVEAN
                     YYYGQSVSDARLHLHVIEFSGDYYDYSDKVLDSRVFPLKDGLASVALQIPRDQKRNVQ
                     VTCTVVDQTGREIEDTVFFRYIPGDAEIDFKLSKYWLPFGETAHGAVRVKGLLPTAEV
                     NRPLTFEVYYDDTFESSSTVYPDPNGALTLAFEPKKPGSYRLRLIDSKYPAYVVERAL
                     FSYSSTYTYRTGKELDVFTNIETASPGDQIIFNVVSSFPSLSVLALIDDGNRASVREI
                     KLKNGNGQLAFFVADSVNSSTLRCRFISFFGSKWLTKNLYVPVDLSHRQAAIHIGVPE
                     RVKPGDTIPLEIRLLNHEEKPVEGAVTLGILDQAVLDLYGDDDWEGLLNELTNPPLRY
                     GFYYFNDSYSYYRLRNQLGFEYDYETPETLAQTKQAAVNRSVKVRTKFSDTAFWTPLW
                     IVSGNEKTEITLPEDLTTWRVRAMAFTKTGLTGYAKASILSTLPVTVNPVFPKFLRTG
                     DETRVGLLVGNSLDQAETFRVSLGLPEEAGGEVLEQTAVIPAKGSRVFWFTITIPEVE
                     QEKSLAFALEAEGESAADAMVHLIPVKPDTMEIPTGQSGLLGLESQSIAYRAETETQL
                     SLTISADLSGELLEALRFLIRYPYGCVEQTVSSFLPTIVARPLIEERPEIKAQEPFAK
                     IEDIIQKGVQKLYGMQNYQGGWGWWAGQSSDAFMTAYALYAFDILRQTGYDINEDVVA
                     MGVGALKNVIKRAKEYEAFCYYVIKSFDPDYPLLLTATDSLETKLFLALSFVKTGETA
                     AASALLKEILAQGTRASPLFYVRFDKNAYFLSAFQKNALLLSLMNGLAYEGPEKNGLI
                     LYLVKNRSGEYWASTKDTSFAVMALVGTELLADQYVWQTEGNLFDYVPEDAQYKRVLQ
                     KGESVVIPVRLRAGDSLELDISGTQGLLWKLTGTERWKLAEYETKTPEAQFSRKLEKL
                     YELKSYSYQTIEKIDTYQFDIYLPWDVPLLPGVIAQKGIPVDIDAVPLTDYELVYAYT
                     DRYKNDVYELYVNGCDTGLAFDESYWVLGFYSDHLVLNKNPWLGYAGQYSLYFVPNRT
                     ALSVGDKVRHRFVFNTEESLAYAVLEDSIPPAYIQSEGEYSYYEGKYFGYGANWLWYA
                     HKEDRYDLTAAFYSVMSEGNT"
     gene            complement(4382..>4850)
                     /locus_tag="PKM49_08540"
     CDS             complement(4382..>4850)
                     /locus_tag="PKM49_08540"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HNY82879.1"
                     /translation="ALQEALRKHDAKWYERVRIPPAAFPDIEAFHYPAVPTLGAQIYQ
                     LKERFYSFANARNLYLYNVDKVSHSLGSEVKPGDLLFLYHPQDTAFPYHVMIYTGDGF
                     VYHTGSSDVSEGEVRLWKTEEYMKAAPLTWLPVKENPNFLGFFRLKILNDDLS"
BASE COUNT         1007 a         1284 c         1394 g         1165 t
ORIGIN      
        1 gtgttccctt cgctcatcac actgtagaat gcggccgtga ggtcgtaacg gtcctccttg
       61 tgggcgtacc acaaccaatt cgctccgtat ccgaagtact tcccctcgta atacgagtac
      121 tcgccctcgc tctgtatgta ggcgggcggt atagaatcct ccagcacggc ataggcgagc
      181 gattcctcgg tgttgaacac gaatcgatgg cgcactttgt cgccgacgct caacgccgtt
      241 cggtttggaa cgaagtagag cgagtattgt ccggcgtaac ccaaccacgg attcttgttc
      301 aggacgaggt ggtcagagta gaagccgagc acccagtagc tttcgtcgaa tgccaatccc
      361 gtgtcacatc cgttgacgta aagttcgtaa acatcgttct tataccgatc ggtgtaggca
      421 taaaccaatt catagtccgt caagggcacc gcgtcgatgt ccaccgggat tcccttttgg
      481 gcgatgacgc ccggcaatag gggcacatcc caaggtaggt agatatcgaa ctggtaggtg
      541 tcgatctttt ctatagtttg gtaggagtag cttttcagtt cgtacagctt ctccaacttg
      601 cgtgaaaatt gggcttcggg tgtctttgtt tcgtattccg ccagcttcca gcgttccgtg
      661 ccggtcaact tccagagtag cccttgtgtg ccgctgatat cgagttcgag ggagtctccc
      721 gcgcgcaggc gaaccggaat cacgacgctc tctccctttt ggagcacccg tttgtattgc
      781 gcgtcttccg gaacgtaatc gaagaggtta ccctccgtct gccagacgta ctggtccgcg
      841 agtagctcgg tcccaacgag ggccataacg gcgaaggagg tgtctttcgt agaggcccag
      901 tactctccgg acctgttttt gaccaggtat aggatgagcc cgttcttttc ggggccttca
      961 taggcgaggc cgttcatcag ggacagcagc agggcgtttt tctgaaaggc gctcaaaaag
     1021 taggcgttct tatcaaatcg cacgtagaat aatggggagg cccgtgtccc ttgcgcgagg
     1081 atctctttca gcagggcgct cgccgccgcg gtctctccgg ttttgacgaa agacaacgcc
     1141 aaaaagagct tcgtctccaa gctgtcagtg gccgtgagta gtaaggggta atccgggtcg
     1201 aaggatttga tcacgtagta gcagaaggcc tcgtattctt tcgcccgttt gatgacgttt
     1261 ttcagcgccc ctacccccat cgccacgacg tcttcgttga tgtcgtatcc ggtttgcctc
     1321 agtatgtcaa aggcatagag ggcgtacgcc gtcatgaaag cgtccgaaga ctgcccggcc
     1381 caccatcccc aacccccttg atagttttgc atgccgtaca gcttctgaac gcctttttgt
     1441 atgatgtcct cgatcttggc gaacggctct tgcgccttga tttccgggcg ttcctcgatc
     1501 agggggcggg ccacgatcgt cggcaggaat gagctgaccg tctgttcgac gcacccgtag
     1561 ggatagcgga ttaaaaatcg cagggcttcg agcaattctc cggaaaggtc ggcgctgatc
     1621 gtcagcgaca attgcgtttc cgtctccgca cggtaggcga tcgattggct ttctaaaccc
     1681 agcaagccgc tttgaccggt cggtatctcc atcgtatcgg gtttcaccgg gatcaggtgg
     1741 accatcgcgt cggccgcgga ctctccctcc gcttcgagcg cgaaggccag ggacttttcc
     1801 tgctccactt ccggaatcgt gatcgtaaac cagaagaccc ggctgccctt cgctgggatg
     1861 acggccgttt gttccaatac ctcgccgcca gcttcttccg gaagccccaa agacacgcga
     1921 aaggtttccg cttggtccaa actgtttccg accaacaggc ccacacgcgt ctcgtctccc
     1981 gttcgaagga acttcgggaa gaccgggtta acggtcaccg ggagcgtgga gaggatgctg
     2041 gctttggcgt atcctgtcaa ccccgttttg gtgaaggcca tcgcgcgtac gcgccaagtg
     2101 gtgaggtcct cgggcaaggt aatttcggtc ttttcgttgc ctgagacgat ccacagcggt
     2161 gtccagaaag ccgtgtcgga aaacttcgtc cgcaccttca cagaacgatt gaccgcggcc
     2221 tgtttcgttt gggccaacgt ctccggcgtc tcgtagtcgt attcgaagcc cagctgattc
     2281 cttaaacggt agtaggagta cgagtcgttg aagtagtaga acccgtatcg aaggggcggg
     2341 ttggtcaatt cgttcaacag accttcccaa tcgtcatctc cgtagaggtc cagtacggct
     2401 tggtccagga tacccagcgt cacggcccct tccacaggtt tctcttcgtg gttcaacagt
     2461 cgtatttcca atggaatcgt atcacctggt ttcacccgct cgggtacgcc gatgtgaatg
     2521 gcggcctgtc ggtgactcag gtccacggga acgtacaggt ttttcgtcag ccatttcgac
     2581 ccgaaaaaag aaatgaaccg gcatctcagg gtagaggagt tcaccgagtc ggccacaaaa
     2641 aacgcgagct gcccgttgcc gtttttgagt tttatctcgc ggacgctcgc tcggttgccg
     2701 tcgtcgatca aggctaacac gctcaacgat gggaaagaag ataccacgtt gaaaatgatc
     2761 tgatctcccg ggctggccgt ttctatattc gtgaagacgt ccagctcttt tccggtgcga
     2821 taggtatagg tggaggagta ggagaagagg gcgcgctcca cgacgtaggc agggtacttg
     2881 gagtcgatca ggcgtaatcg gtaagagccg ggctttttcg gttcaaaggc gagggtcaac
     2941 gcgccgtttg ggtcggggta cacggtggac gaggactcga aggtgtcgtc gtagtacacc
     3001 tcgaaggtca gggggcggtt gacctcggcg gttggcagga ggcccttgac gcgaacggcc
     3061 ccgtgggcgg tttcgccaaa gggtagccag tatttagaga gtttgaagtc gatttcggcg
     3121 tcgccgggga tgtaccggaa gaaaacggtg tcttcgatct cccgacccgt ctggtccaca
     3181 acggtgcagg tgacctggac gttccgtttt tggtctcgcg ggatctgcag cgctacggaa
     3241 gcgaggccgt ctttgagagg aaagacgcgg ctgtccagca ccttgtcgga gtagtcgtag
     3301 tagtctccgg aaaactcaat gacgtgcagg tgaagtcggg cgtcggacac actctgtccg
     3361 tagtaataat tggcttccac acggagctct atcggctcgt cgagcgcgta gacgggtttt
     3421 tgcagcgagg cgttcacggt aaatgacggt ttggcgtagt cggccagttc gaagtagcgg
     3481 tagctcacat catccccgta tctgatcttg acggtgtagt gccctcgagt gtcgtccggg
     3541 aaagtttcat atataaaact gatccctccc cactcgtcgg agtgaaagac gcccttcatc
     3601 ttctctcttc cgagcgggtc cacgatggcg acttctatgg ggcggttggt caacagctct
     3661 ccagttttgg agtttaaaaa gatggcccgc gcctgaacca agtcgcccgg gcggtacaag
     3721 gggcggtcgg ttaaaaagat caggggatcg agccgctcgt ccgcttggcg aagggcgtcc
     3781 gagaggaaca ggatgccatt cggagtttcg acgaacagcg tttcctccac gattttcgat
     3841 agctctattt cgacgagacc ggcggatggg gcgattgaca ggggtttggg agaggtgtag
     3901 cccgtgagaa tctgtgtaaa tcgcacatcc tttccctcga tgttgtccca gagctttatg
     3961 tagacggact ctcctatact caccagagct ccgttcacat tcgtcttcag tagtagggta
     4021 tagtcttttc tgtcttttga aacgtattcg aggacgtagg ttccgaccct ctcgaaatag
     4081 ccggaaggga tttccaagcg cttccaagcc ttcgcctctt cgaaatacac cgcgtgatcc
     4141 gcctgtttag gaggcttttt taacgtgttg cgcttgaatt ccgtcaaaaa atcctggagt
     4201 gtgggtccga tgtagtaaag gttgacggtg accccgtcgg gctcgttgta cgcccaaaac
     4261 gagtaagatt gatacggata caggatggcg tttctgtccg cagagatgta accgaacgcg
     4321 acacttgata tcgcgataag caaaagaaag agccttacgg cgtttttcat caaatccccc
     4381 cttatgaaag atcgtcgttc aatatcttaa gacggaaaaa acccaggaag ttcggatttt
     4441 ctttaaccgg aagccacgtc agcggagcgg ctttcatata ttcctcggtt ttccagagtc
     4501 gaacctcccc ctcggaaacg tcggaactcc cggtgtgata gacgaaaccg tcaccggtgt
     4561 atatcatgac gtggtacggg aaggcggtgt cttgcgggtg gtacaaaaag aggaggtctc
     4621 ccggcttcac ttcgcttccc agcgagtggc ttactttgtc cacattgtac aagtagaggt
     4681 ttcgggcgtt tgcgaagctg tagaacctct ctttaagctg gtaaatctga gcgccgagcg
     4741 tggggacggc ggggtaatga aaggcttcga tatcggggaa agctgcaggc ggaatccgaa
     4801 cccgctcgta ccacttggcg tcgtgtttcc ggagcgcctc ttgcagggcg
//
LOCUS       DAOPXN010000174         4775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17937_length_4775_cov_5.841525, whole genome shotgun sequence.
ACCESSION   DAOPXN010000174 DAOPXN010000000
VERSION     DAOPXN010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17937_length_4775_cov_5.841525"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PKM49_08545"
     CDS             <1..1016
                     /locus_tag="PKM49_08545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82880.1"
                     /translation="VRHISVLIPARNEEHNIGDCVRSILQNDYPFYEVILMDDGSTDA
                     TSQIAKGFNDPRLRVLRAPDKPDGWVGKNWACHQLSLEARHPILLFMDADTRLTPDAL
                     WRINAVFTLTDADVLSGCPKQIPTSFLDRVLLPIMPLLPMATLPLFKIGDFRFVRSAI
                     HGAFIVIRKDFYQKTGGYETIKGQWVDDAALNKVLVKRRSKTEMLDMTKIVSCRMYTS
                     PKDTIEGFARSLYHELFGMVPLIIFLMAMLFLATIVPFLLLIFSRSVEQLALSLWVIG
                     VITGIVWVTNRKYGFPWYSAFLLPLMFFTVAYTAYKSIQGSRKKNMTWKGRPITDERP
                     SPR"
     gene            1672..2799
                     /locus_tag="PKM49_08550"
     CDS             1672..2799
                     /locus_tag="PKM49_08550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82881.1"
                     /translation="MVHWVFFWVSIGFLCYFTLNLLTYTRFNRVLRLAEKDRREPVRA
                     FSILIPARNEESNIEACVRAALKNDYPNYEVIVMDDGSTDDTYEIVSSIGDPRLKVFR
                     APQKPTGWAGKNWACHQLSLKATAPFFLFIDADALLAPDALWKINEVFNRTEAEVVSG
                     CPKQIPSSIVDLILLPVAPYLPLASMPLFKIGNFRFVRTAIHGALVAYDKRFYLKFGG
                     HEAIKDQWVDDAALNKIIPKYGGKTEMLDVTYIVSCKMYDNGKATIEGVARSLYHSLY
                     GKISLVVFIMFILFLITLFPWLLLFFSRTLTQLALSLVTLAIMTGTWLVVDLKYGFPA
                     YTVLLLPLTFFFSVYVSFISVRRSLKKDFLWRGRPVDPKGS"
     gene            3395..>4775
                     /locus_tag="PKM49_08555"
     CDS             3395..>4775
                     /locus_tag="PKM49_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82882.1"
                     /translation="MPEKIIYALPEQIGNIELFVGRKKEFDYYLGDWYRYLVKNMAQS
                     QAIVARRKKGKTAFLQRLFNILWSCSESKVIPFYYVIQDKPVTLSGFAEDFFTQFMWQ
                     FLSFRDRKEEYLSYPIGFEKLAPLVKGDKYLTDAYETMAIKKKEDDWDGMWNTASRLP
                     AALATVKKMKIVQIIDEFQNINTYIVDERRNTIDSLSGTYRDLAEKKEAPLIISGSEV
                     HGLMKIIQNLTARFKKRTLGNLPEEEAKEAIRRYAYVSQTKINEQAEEKIWNLTQGDP
                     LYIRALMQSEHNDTKDYTNEDNIIATYTQEITQGEIYGTWMEYIAKIFLEVNKRNAKR
                     LLLYLFQAGEERTRAQMIKDLKLDMTDSELETKLLQLIKADLISQGQTNFDYKIAKDK
                     TYELVFRRLYQKEIDYFVPDIKKELRQEMGRTSYEKGKFREYLVRERIKKPFNLKDLA
                     ENGIDLTIKP"
BASE COUNT         1251 a         1283 c         1178 g         1063 t
ORIGIN      
        1 ccgtgcgcca catttctgtc ctcattcccg cccgaaatga ggagcacaat atcggtgatt
       61 gcgtacgctc gattttacag aacgactacc cgttttacga agtgatcctg atggacgatg
      121 gctccaccga cgctacttct cagatcgcca aagggttcaa tgacccgagg ctccgcgtgc
      181 tgcgcgcgcc ggataaaccg gacggctggg tggggaagaa ttgggcctgc catcagctct
      241 ccttggaagc gagacatccc atcctcctct ttatggacgc ggacacccga ttgacgccgg
      301 acgcgttgtg gaggatcaac gccgtcttca cgttgaccga cgcggacgtc ctctccggat
      361 gccctaagca gattcccacg tccttcctcg accgcgtgct gctcccgatc atgcccctcc
      421 tcccgatggc caccctgcct ttattcaaaa ttggtgactt ccgtttcgtg agaagcgcta
      481 tccacggggc tttcatcgtc atacgcaaag acttttacca aaaaacgggc ggatacgaaa
      541 cgattaaagg ccaatgggtg gacgacgccg ccctcaacaa agtactcgtc aagcgccgct
      601 caaaaaccga aatgctggat atgacgaaaa tcgtctcctg ccggatgtat acatcgccga
      661 aagatacgat cgaagggttc gcgcgcagcc tctatcacga gctcttcgga atggtccccc
      721 tgatcatatt cttgatggcg atgctcttcc tcgcgacaat cgtgccgttt ctcctcttga
      781 tcttcagccg ctcggtcgaa caactggcgc tcagcctctg ggtgatagga gtcatcacag
      841 gtatcgtgtg ggtaaccaat cgcaaatacg gcttcccttg gtacagcgcg ttcctactcc
      901 cgctgatgtt tttcaccgtg gcgtataccg catataaatc gattcaaggc tcgagaaaaa
      961 agaacatgac ctggaaaggc cgccccatta ccgacgagcg accttcgccc cgctagcgcc
     1021 cgggaacacc gaactttagt tcggcatgtc ctttgaaaaa caaaagaagt ttcgcacaga
     1081 gaacacggag tacacagaga gaacacggag aaacgccctg ggaacgccga acttcatagg
     1141 tcatcgcggg gtaactaaca ggaccccgcg atccgctcta tgtcctcaga aaaagggtat
     1201 cgcgccgtcg cgaaaagaag aaccgcttac gccgcgatgc cgcgcttggg aacgccctgg
     1261 gaacaccgaa ctttagttcg gcatgtttat tggaaaaaca aaagaagttt cgcacgaaga
     1321 acacggagta cacagagaaa ctccctggga acgccgaatt tcataggtta tcgcggggta
     1381 accaacgggc tgacttcgga agcctacgaa ctcgcgcgat ccggcttttg cctttcacaa
     1441 aagcgtatcg cgccgtcgcg aaaagaagaa gcgcttacgc cgcgatgctg cgcttgggaa
     1501 cgccctggga acgcctacca cctccgtgcc ctctgtggcc tctgtgcgaa ccccaaaacc
     1561 cgtgccgcac tatagttcgg cgttccgata gcgctcgctt tgttgacttg cccgttccaa
     1621 aaaggtagaa tcagctttaa agaagctgtt ataaggaaag gggctgtgag gatggtccat
     1681 tgggtttttt tctgggtttc gatcgggttc ttatgttatt tcacgttgaa tctgctgacc
     1741 tacacccggt tcaatcgagt gttgcggctt gcggaaaaag atcggcggga gccggttcgc
     1801 gccttctcca tcctcatacc cgccagaaat gaagagagca acatcgaggc gtgtgttcgc
     1861 gcggcgctta aaaacgacta cccgaactac gaggtgatcg taatggacga tgggtccacg
     1921 gacgacacct atgagatcgt gtcgtctatc ggtgaccccc gattgaaggt tttccgcgcc
     1981 cctcagaaac ctaccggttg ggcgggaaag aactgggcgt gccaccagct ttcgctgaaa
     2041 gccaccgcgc cgtttttctt gttcattgac gccgacgcct tgctggcacc cgacgccctg
     2101 tggaagatta atgaagtctt taaccgcacg gaggcggagg tcgtttctgg ctgcccaaaa
     2161 caaatcccga gctcgatagt cgatctcatc ttgcttccgg tcgcgccata cctccccctc
     2221 gcttcaatgc cgctcttcaa aatcggaaat tttcgcttcg tgagaacggc catacacgga
     2281 gcgctggtcg cgtacgacaa acgcttttac ctgaaatttg gtggccacga ggcgataaag
     2341 gaccaatggg tggatgacgc cgcgctaaac aaaatcattc cgaagtatgg cggaaaaacg
     2401 gagatgttag acgtgaccta tatcgtttcc tgtaagatgt atgacaacgg gaaagcgacg
     2461 atcgaaggcg tggcgcgcag cctctaccat tcgctctacg gcaagatatc gctggtggtc
     2521 ttcataatgt tcatcctgtt tctcatcacc ctgtttcctt ggctcctcct ctttttcagt
     2581 cgcaccctca cgcagttggc gctgagcttg gtcaccctcg cgattatgac cggcacctgg
     2641 ctcgtggtcg acctcaaata cggtttcccg gcgtacactg tcttacttct tccgctcacc
     2701 ttctttttct ccgtgtacgt ctctttcatt tcggtgcgcc ggtcgctgaa aaaagacttc
     2761 ctttggcgcg gacgccccgt cgacccgaag ggttcgtaaa aaacgttccg gtacaccaaa
     2821 agaagtttcg catagaaaac accgtgcgat cacctgggaa cgctgaagtt tagttcggca
     2881 cgtttttcca aaaccaaaag aagtttcgca cagagagaac acagagaact gccctgggaa
     2941 ccccctggga acgccgaact ttagttcgac acgttttttg gaaaaacaaa agaagtttcg
     3001 cacagagaac acggagtaca cagagaaaac aataaccaga tgttgactct cgtaaaaatc
     3061 tctctccttt gtgtcctcag tgccctccgt ggtctctgtg tgacccctgt tttttatgac
     3121 ctgccaacat aatagttgtc gttcccagaa cacacgccgg cagggaagtc ggcgccacca
     3181 ggtgctctca gtaatccctc ctttgtgccc tctgtgccct ccgtgtgacc cccttttttt
     3241 caaggcatac caacttaaaa gttggagtcc ccagaaaacc ttcctttgcc ctctgtgcga
     3301 accctttttt ccttccccat ggcgctccca gccttacgct ctatgctata atctttccaa
     3361 actgaaacga agcggggtga aagcgatgtt cacgatgccg gaaaagataa tctatgcctt
     3421 accggagcaa ataggaaaca tcgagctctt cgtcggccgt aagaaggagt tcgattacta
     3481 tctgggagat tggtaccgat acctcgtcaa aaatatggct caaagccaag cgatcgtcgc
     3541 caggcgaaag aaagggaaga cggcgtttct gcagcggttg ttcaatatcc tatggagctg
     3601 ttcggaaagc aaggttattc cgttctatta tgtcattcaa gataaacccg tcacgttgtc
     3661 tgggttcgcg gaggatttct ttacccaatt tatgtggcaa ttcctatctt ttcgagatag
     3721 gaaggaagag tatctttcct atccgatcgg ctttgaaaag ttagctccac ttgtaaaagg
     3781 cgataagtat ttaacggatg cgtacgaaac gatggcgata aaaaagaaag aagacgattg
     3841 ggatgggatg tggaacacag ccagcaggtt gcccgcagcg ttggcgacag ttaaaaagat
     3901 gaaaatcgtc caaatcatag acgaatttca aaatatcaac acctatatcg ttgatgagcg
     3961 aaggaatacg atcgactcgc tgagcgggac ttaccgtgac ctcgcggaaa agaaagaagc
     4021 gccgctcatt atttcgggga gtgaagtcca cggattgatg aagatcatac aaaaccttac
     4081 cgcccggttc aaaaaacgaa cgctcgggaa cctcccggaa gaggaggcaa aagaagcgat
     4141 ccgacgatac gcttacgtat ctcagaccaa aatcaatgaa caggcggaag aaaagatatg
     4201 gaatctgacg caaggggacc cgctgtatat ccgcgcgctg atgcagagcg agcacaacga
     4261 tacaaaagac tacacgaacg aagacaacat catcgcgacg tacacacagg agattactca
     4321 aggagaaatc tacggcactt ggatggaata tatcgcgaaa atctttttgg aagtgaacaa
     4381 aagaaacgcg aagcggctct tgctgtatct gtttcaggcc ggagaagaac gcacccgagc
     4441 gcaaatgatc aaagacctca agctcgatat gaccgactcc gaactggaaa cgaagctgct
     4501 ccaattgatc aaagcagacc tgatctcgca aggtcaaacg aacttcgatt acaagattgc
     4561 gaaagataaa acctacgaac tcgtatttcg acgcctctat caaaaagaga tcgactactt
     4621 tgtccccgac ataaaaaagg agctgcgtca agagatggga aggacgagct acgaaaaggg
     4681 taagttccga gaatacctcg tacgagaacg gatcaagaaa ccgttcaacc tgaaagacct
     4741 cgcggaaaac gggatcgatc tcaccatcaa accga
//
LOCUS       DAOPXN010000175         4773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_17951_length_4773_cov_6.138618, whole genome shotgun sequence.
ACCESSION   DAOPXN010000175 DAOPXN010000000
VERSION     DAOPXN010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17951_length_4773_cov_6.138618"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1599
                     /locus_tag="PKM49_08560"
     CDS             193..1599
                     /locus_tag="PKM49_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNY82883.1"
                     /translation="MKKSIPVFLWVFVLSCALGFGQVFYLAVVQAQEAFPEQAFRLRY
                     FLNNQYTGAEKIVVCSGNTQVTTLFGLYSQIFSPTPDKTPWNALFEMLEIEAALPTRT
                     DLAAGVSAIAQSLPASVKIVATDSQETSGSRLYYPRTIGKKNILFANITETPSNWYER
                     LRKEVTFLSYDAIVLVVDNPDFSIVPESLRSKTMVIPRKTAIYRVDLEYGLLITEMKI
                     PTEKEIPEGLKTLQADFSRWTEQPLPIDPDGKCFLYEIPFYSMMGIMMNNLFPMDLAI
                     FCQRSLPLQATPQETIAFFERDVFGTLFLDNSSIRKLLERAASALDIVDGEIRFRSPA
                     DFFSVWGEPYFLDLTKPKGERLIMRTFDKPQRIGFFGPRDQLEKTVPGIRILDISPLP
                     YIFWAMKYGNVNLDPAWRTLTQPYYFDYVIQKGDTLIGIAKAYGVTVEEIKRDNPGVI
                     DMYLLPGTVLKIHLPIGD"
     gene            1749..3275
                     /locus_tag="PKM49_08565"
     CDS             1749..3275
                     /locus_tag="PKM49_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNY82884.1"
                     /translation="MEIILFLVILTIVVLVHELGHFLFAKLFKVKILQCGIGFGPKIY
                     SRTGKDGIEYRINIFPLGGYVRMAGEDPTETHHTPPEERQDYFYAKPAWQRFLIAFSG
                     PLFSILFGYLFMTMTCAIWGVPTIAVERVGLDTPAERAGLRSGDILYRVNGRRIFGLE
                     DFSDAVKKEERFTATIIRKGETHRLEIVPEVFSEQTNYVFETELPLPNLEGKTLLSIN
                     GIPLSIADLKNLTEQPVDLMIEGGEAVPITLKAFTTLNPQKMIGIVYSTLSTTLKDTA
                     YPFMKEDRILSINGVKLERGGDFYPALTLFAFTQKENRYFTYLETRGGLVTHQYGQPS
                     VKKVTVLVERNGLPTQLEMPYQDFTEALENSLFYPANENLYPDNLWQAIGLGIQRTNG
                     MLTSMGSIIRNLFSGKASVKDFAGPIGLVNIIGQASRAGLETLIFLLAFITLNLGIIN
                     LLPLPALDGGRIVFSILEMITRKRINPVIEGYIHTIGFFLLIGFAIYISYFDIIRFMR
                     "
     gene            3378..4031
                     /locus_tag="PKM49_08570"
     CDS             3378..4031
                     /locus_tag="PKM49_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82885.1"
                     /translation="MKRKGLFLFLLLLLPLFAFPQTEGEDTLHIKADLFKFFDDRIEF
                     YNHAEIIKGEFSLVSEKFFKILRERGEDRKVEAKQGIFVEFETGRATAIELDYDLTEE
                     KGTLKKDVHTIIKPGESEGTVEVFCDIMDFETRAKTYKGKNDEAGKLVKIYKGNLYAE
                     CREFVYTGDTGIIELFGKVYVYDPDNRRKLWGEYVKLFLESDTLEAKNAVMEVITKK"
     gene            4062..>4773
                     /locus_tag="PKM49_08575"
     CDS             4062..>4773
                     /locus_tag="PKM49_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY82886.1"
                     /translation="MVCPTCKKAAPDSMAYRCPDCGEPFELEFEARFPIDKIRKRKAN
                     LWRYREAIPIESDEDIVSFEEGFTPLLKFDILGHPVWVKQDHLFPSGSYKDRGASVLI
                     SYVRSMRIENVVEDSSGNAGCSIAMYATAAGIECEVFVPQVTSPAKMEQARMLGARLR
                     KIPGSRQETAQAVLEEAKRAYYASHVYNPYFLQGTKTLAYEIVEQMEWTAPDSLILPI
                     GNGTLLLGVYNGFKDLIQA"
BASE COUNT         1189 a         1260 c         1250 g         1074 t
ORIGIN      
        1 ttacgataaa agttgtttat taagtacgtc ttgtaaattg tcgaagagcc tgttttttga
       61 ttacacctca taacttgacc cacgccaaag gatacaattc gcgatagaga gtgttataat
      121 ctagggttct acgaaattac tccgaagaaa cgttgcgggg actccttccg tttctttgaa
      181 gaggaggacg gcatgaagaa aagcatacct gtgttcttat gggttttcgt actctcgtgc
      241 gcgcttggat tcggacaagt cttctatctc gccgtcgttc aagcgcaaga ggcctttccg
      301 gagcaagcct tccggttgcg ctattttttg aataaccagt acaccggcgc cgagaagatc
      361 gtggtgtgca gcgggaatac ccaagtgacc acgctgttcg ggctgtactc tcaaatcttt
      421 tcgcccactc cggataaaac gccctggaac gcgctcttcg agatgctcga aatagaggcc
      481 gcgctgccga cacgaacgga tttggccgcc ggcgtttccg cgatcgcgca atcgcttccc
      541 gcgtccgtca agatcgtggc caccgatagc caagagacgt caggatcgag gctctattac
      601 cctcggacga tcggcaagaa aaacatcctg ttcgccaaca tcaccgaaac gccttccaac
      661 tggtatgagc ggcttcgcaa agaggtaacc ttcctctcct acgacgctat cgtcctcgtc
      721 gtggacaatc cggacttcag catcgtgccg gagagcctgc ggtccaaaac gatggtcatc
      781 ccgagaaaga ccgcaatcta tcgagtcgat ctggaatacg gcctactgat caccgaaatg
      841 aagattccga cggaaaaaga gatccctgag ggcctgaaaa cacttcaagc ggacttttcg
      901 cgctggacgg agcaacccct cccgatcgat cccgacggaa aatgctttct ttatgaaatc
      961 cccttctata gtatgatggg gatcatgatg aataacctgt ttccgatgga tttggcgatc
     1021 ttctgccaac ggagtctgcc gcttcaggcg acgccccagg aaacgatcgc ttttttcgaa
     1081 agagacgtgt tcggcacgtt gtttctggac aactccagca tccggaagtt gttggagcgg
     1141 gcagcctccg ccctggatat cgtcgatgga gagatacgat tccgttcgcc cgccgacttc
     1201 ttttccgtgt ggggggagcc ctatttcctc gatttgacga agccgaaggg cgagcgactg
     1261 attatgagaa ccttcgacaa accccagaga atcggctttt tcggccctcg ggaccagctg
     1321 gaaaaaaccg tcccagggat cagaatcttg gatatttccc cgctgccgta cattttttgg
     1381 gcgatgaagt atgggaatgt caatctggac ccagcttggc ggaccctgac ccaaccctat
     1441 tatttcgatt acgtcataca aaaaggcgat acgctcatcg ggattgcaaa ggcctacggt
     1501 gtgacggtgg aagagatcaa acgcgacaac cccggggtga tcgatatgta cctcttaccg
     1561 ggaacggtgt tgaaaatcca ccttccgatc ggagattagc gctcattcga cgatcaggcg
     1621 tcagcgatcg gatgcgctcg agtttgccta gcgtcagtat ccgctctcgc gtctggaacg
     1681 gcgcgcatca aatccaggat acgcgcgcgg gattccacga aagaaatcgg cggtaaagga
     1741 gaagcatttt ggaaatcatc ttgtttcttg tgattctaac gattgtggtg ctagtccacg
     1801 aacttggaca ttttcttttc gcgaaactgt tcaaagtgaa gatcctccaa tgcggaatag
     1861 gatttggccc gaagatctat tctagaacgg gtaaggacgg gattgagtat cgcatcaata
     1921 tctttccgtt ggggggatac gtgcgtatgg cgggtgaaga cccgacggaa acccatcata
     1981 cccccccgga ggagcgccag gactatttct atgcgaaacc ggcttggcag cggtttttga
     2041 tcgcgttttc cggcccgctt ttttccatcc tgttcggcta tctctttatg acgatgacct
     2101 gtgccatctg gggcgtcccc accatcgcgg ttgaacgcgt ggggctcgat acccccgcgg
     2161 agcgcgcggg gttacggtcg ggagatattt tgtaccgtgt caacggccgt aggatattcg
     2221 ggctggagga cttttccgac gcggtgaaaa aggaggaacg gttcacggcg acgatcatcc
     2281 ggaaaggtga aacccaccgg ttggagatcg ttcccgaggt tttctcggag cagaccaact
     2341 atgtgttcga aacagagtta cctttaccaa acctggaagg aaaaacgctt ctatcgataa
     2401 acggaatccc cttatcgata gcggacttga aaaacctcac cgagcagccg gtcgatctca
     2461 tgatcgaagg cggagaagcg gtcccgataa cgctcaaggc cttcaccacg ctgaaccctc
     2521 aaaagatgat cggcatcgtg tactccaccc tttcgaccac tttaaaagac acggcctatc
     2581 cgtttatgaa agaggatcgt atcctctcga tcaatggggt gaagctcgag cggggcggag
     2641 acttttatcc cgccctcacc cttttcgctt tcacacaaaa ggaaaaccgt tatttcacct
     2701 acctggagac gcgcgggggc ctcgtcaccc atcagtatgg ccaaccctcc gtcaagaagg
     2761 tcaccgtcct cgtggagcgg aacggccttc cgactcaact tgaaatgcct tatcaagact
     2821 tcacggaagc gctggaaaac agcctgttct atccggccaa tgagaaccta tatcccgata
     2881 acctctggca ggcgatcggg ttgggcatcc aacgtacaaa cgggatgttg accagtatgg
     2941 gaagcatcat ccgcaatcta ttttccggaa aagcgagcgt caaggatttc gcgggcccga
     3001 ttggactggt gaatatcata gggcaagcgt ctcgggcggg gttggaaacc ctgatcttcc
     3061 ttctcgcctt tatcacgctc aacctcggca tcatcaacct gctcccgtta ccggcgctgg
     3121 acggcggacg gatcgtgttc agcatcctgg agatgatcac gagaaaaagg atcaaccccg
     3181 taatcgaagg ctacatccat accatcggat ttttccttct gatcggattc gccatctata
     3241 tctcgtattt cgacatcatc cgcttcatgc gctaaacgaa atatgcactc gctccggtta
     3301 aaacggatag agccaccaag gagaattgtc actatctcaa gaaataaagg aggtgtccgg
     3361 cgacaagccg ggtagctttg aaaagaaagg gcctattcct atttttactg ctcctcttgc
     3421 cgctcttcgc cttcccccaa accgagggcg aagatacgct ccatattaaa gcggacctgt
     3481 tcaaattttt cgatgaccgc atcgaattct acaatcacgc cgagatcatc aaaggcgagt
     3541 tcagcctggt ttcagaaaag ttcttcaaaa tcctcagaga acgtggggaa gaccgtaagg
     3601 tggaagccaa gcaggggata ttcgtggagt tcgaaaccgg tcgcgccacc gccatcgagt
     3661 tggactacga cctcaccgaa gaaaaaggga cgctcaaaaa agatgtccac accatcatca
     3721 aaccgggcga gagcgaggga acggtggaag tgttttgcga tattatggac ttcgaaaccc
     3781 gagccaagac gtacaaggga aaaaacgacg aagccggcaa gctggtcaag atctacaaag
     3841 ggaacttgta cgccgaatgc agggagttcg tctacacagg ggataccggc atcatagagt
     3901 tgttcgggaa ggtctacgtt tacgatccgg ataaccggcg taaattgtgg ggcgagtatg
     3961 tgaagctctt cttagagagc gacacgctgg aagcaaaaaa cgctgtgatg gaagtcatca
     4021 cgaagaaata aacgcaaaag ggaggtctca cgttgatacg aatggtctgt cccacctgta
     4081 aaaaggcagc tcccgattcg atggcctatc gttgccccga ctgcggagaa ccctttgaac
     4141 tggagttcga ggcgcgtttt ccgatcgata agatccgaaa acggaaagcc aacctttggc
     4201 gctatcggga ggcgattccc atcgaatcgg atgaagacat cgtctctttc gaggaagggt
     4261 tcactcccct actgaagttt gacatcctag ggcacccggt ttgggtcaaa caggatcact
     4321 tgttcccgag cggctcctat aaggatcggg gggcctccgt actcatcagt tacgtccgtt
     4381 cgatgaggat tgaaaacgtg gtcgaagatt cgtccggaaa cgccggttgc tctatcgcga
     4441 tgtacgccac cgcggccgga atcgaatgcg aagtcttcgt accgcaggtg acctcgcctg
     4501 ctaaaatgga acaagcgcgg atgttgggcg cccgcctacg gaaaatacca ggctctcgtc
     4561 aagagacggc gcaagcggtc ttagaagagg cgaagcgcgc ttattacgcc tctcacgtct
     4621 acaaccccta ctttttgcag ggaaccaaaa ccctcgcata cgagatcgtc gaacagatgg
     4681 aatggaccgc gccggattct ttgatcctcc ccataggcaa cggcacgctc ctgctcggcg
     4741 tgtacaacgg cttcaaagac ttgatccagg cgg
//
LOCUS       DAOPXN010000176         4744 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_18101_length_4744_cov_4.883771, whole genome shotgun sequence.
ACCESSION   DAOPXN010000176 DAOPXN010000000
VERSION     DAOPXN010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18101_length_4744_cov_4.883771"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..597
                     /locus_tag="PKM49_08580"
     CDS             211..597
                     /locus_tag="PKM49_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82887.1"
                     /translation="MKKLTLVVAFLLIALVGFSWLSTIPARNYGWALPALDSPYYLNR
                     AVYPYYGYGSVYPVAPSVYSYAVAPSVYTVPVRTYYPTVVSTVPAYVVPVVRYPVAYY
                     APVVYSPWVPVSYYPWGTYYPTLYGW"
     gene            607..1344
                     /locus_tag="PKM49_08585"
     CDS             607..1344
                     /locus_tag="PKM49_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82888.1"
                     /translation="MKSGLFIVLFIVILAVGGFGAIFIMRDASTHSGTILQVTETAFI
                     VQGENGQRMELPRSEVVSILTESLSGSVSQTSSASPIMAQPVPEVGYPAPVASAQIAV
                     ESPYYTIDMEFESRKRVLLYQERAKNPLLSVGLSLVIPGGGHFYTERWGAGVFFFATR
                     AIFTGVTLWGFWTTATVDENDEPVFRMNSPIVGTVGAVGLTTMLILECVDSFSGVNRY
                     NDMLRVRLGIDKVDTQLIPAMPAIVSP"
     gene            1651..2838
                     /locus_tag="PKM49_08590"
     CDS             1651..2838
                     /locus_tag="PKM49_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNY82889.1"
                     /translation="MIYLDHYRTTPVSEAVVSKMAPFFRDDFFLPEPFTKRGTKAEEI
                     IEQANRTLLDTFHLKTGEVMYTSGGTAANNIVISGVLRDVEPSQAHAVCAVIDHPSIV
                     HTYEYYRKKGVSVTFLKVDDDGWIDPEELRWSIQPKTLLVSFTHVNHTIGVIQPLEQL
                     LPIIKERNPNTRIHIDAAVSLNAMDLDLTKLPIDFLTFSGHKIYGPKGIGAVVFRNNG
                     LLKPTLFGSVTTSPFTPGAENIPGIVGLAEALRLAAEKRATYRLKTRRLQERAINLIE
                     SDIPDVVLNGPKPTSFGNLDAIPTQRAVDNINFSFRYVEGESIMLFLDFEDIVVATGS
                     ACASSDLKVNYVLSAIGRDHEMAHGSLRITPGWDNDEEQIDRFVDRLKPIVSRLRAQS
                     TLR"
     gene            2989..3615
                     /locus_tag="PKM49_08595"
     CDS             2989..3615
                     /locus_tag="PKM49_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNY82890.1"
                     /translation="MRYSEKVLEHFRNPKNIGLIENADGQSTVGSPACGDQVSMFLKV
                     EEDRIADIKFLSYGCASNIATGSIITEMAKGKTLAEAKAITWKDAVDELGGLPPVKIH
                     CSVLAIDGLRAAIQDYEEKTTGVKSERKVDEMAILDELKKVIYPKNGINIVDTNMIRY
                     LSFEEGTIYIEVSLEETDVFKQNVLEEIYEHLEALPGVDKVEIKVVEY"
     gene            3615..>4744
                     /locus_tag="PKM49_08600"
     CDS             3615..>4744
                     /locus_tag="PKM49_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HNY82891.1"
                     /translation="MREVYLDHAATTPPYPEVLKALQESYATIYGNASSLHATGQKAR
                     RALEKAREDIAKLIDAEPEEVYITSGGTESDNIALFGTEALYPGQPMITSLIEHEAVL
                     ESAKALESKGHPVRFLPVDTNGVVDLNALKAYLEEKPFLVSVMAANNEIGTVQPVKEI
                     AKLAHERGALVHTDAVQAVGKIPINVRELGVDMLSASAHKFYGPKGIGFFYLRKGVKV
                     LPIVQGGGHERGLRSGTENVPGAIAMALALKISTRLFTRTHYRYERWTAQILSKCGEI
                     GGFRLNGHPQQRVPGLLSLSFKRINGEALMELLDMNGVAVSTASACQSHSAKRGYSHV
                     IQSLGIDEEYANGTIRVSLGVENVQEEVDFFCEILKKCVLQL"
BASE COUNT         1155 a         1190 c         1305 g         1094 t
ORIGIN      
        1 ttgagaaagt ggcttttatc gccgtttgta gcacttttag tcgaaagttg ggttataccc
       61 acaccgcctg ttgcattcct attggcgtta tggtataatc ttttcacgta aaataaaaga
      121 agaataccgt tatatgttca ttccgctttg agaaatgatg cataagcaga cactggaagg
      181 acaaaaaaga ttgcgaaagg gggttcatct atgaagaagc tgacacttgt agtggctttc
      241 ctattgatcg cgttggtcgg tttttcctgg ctctccacca tacccgcaag aaactatggc
      301 tgggcgttac ctgctctgga ttcgccttac taccttaaca gagctgtata cccttattac
      361 gggtatggat cggtctaccc ggttgcgcct tcggtctatt cttacgcggt agcgccttcg
      421 gtctataccg ttccggttcg cacctattac ccgacggtcg tctcaacggt tccggcctac
      481 gttgtgcccg tcgtacgtta cccggtcgcc tattacgcgc cagtagtcta ctctccttgg
      541 gtgccggtca gttattatcc gtgggggacc tattatccca ccctctacgg ttggtagatg
      601 atcgttatga agtctggcct tttcattgtc ttattcatcg ttatattagc cgttggaggc
      661 ttcggcgcca tcttcattat gagagacgcg agcacccact cggggacgat acttcaggtg
      721 accgaaaccg ccttcatcgt ccagggagag aacgggcaaa gaatggaact gcctcggtcc
      781 gaggtcgttt ctatactcac ggagtctctc tcgggttccg tttcacagac ctcttcggct
      841 tcacccataa tggcgcagcc ggtccctgag gtgggttatc ccgcgcctgt cgcaagcgcg
      901 cagatcgccg tggaaagccc gtattacaca atcgatatgg aatttgaaag ccgcaaacgc
      961 gttttgctct accaagaacg cgcgaaaaac ccgctgcttt ccgtcggcct cagtttggta
     1021 atcccaggcg gggggcattt ctacaccgaa cgctggggag cgggcgtctt ctttttcgcg
     1081 acgcgcgcca tcttcaccgg agtcacccta tggggcttct ggactaccgc gactgtcgac
     1141 gagaacgacg aacctgtctt taggatgaac agccctatcg tcggaaccgt tggcgcggtg
     1201 ggactcacca cgatgctgat actcgaatgt gtggactctt tttcaggggt gaatagatac
     1261 aacgatatgc ttcgcgtccg tttgggcatc gataaagtgg atacgcaatt gatccccgcg
     1321 atgcccgcga tcgtcagtcc ctaaagacga atagacgagc ctaatcgccg cggccttggc
     1381 cgcggttttt tttgaaaaga acgctacccc tctgggaacg ccgaacttta gttcggcttg
     1441 ggcttgcctt ttggttggct gggaacgacg gactgtagtt cggcttgggt ttggaaggcg
     1501 ctcccaggcg gtgcttccgg aaggcgctcc tagtaaaaac cacgtcgcaa cggagaaaaa
     1561 agtagccaat ctcaaaggca gaggacaaca ccgtttttcg tcttttgtgg tagtataata
     1621 agatgtcgag acgaaggagt tgaacaaccc ttgatttacc tggatcatta tcgaacgaca
     1681 cccgtttccg aggcggttgt cagcaagatg gccccgtttt tccgggatga tttttttctt
     1741 ccggagcctt tcacaaaaag ggggacgaaa gcggaggaga tcatcgaaca agcgaatcgg
     1801 acactattgg ataccttcca tctgaaaacc ggagaggtga tgtacacctc cggcggtacc
     1861 gccgccaaca acattgtcat ctcaggggtc ttgagagacg tggaaccgtc gcaggcgcac
     1921 gccgtttgcg ccgtcatcga tcacccttcg atcgtccata cctacgaata ctatcgcaag
     1981 aaaggggttt cggtgacctt cctcaaagtc gacgatgacg gatggatcga tccggaggaa
     2041 ttacgctgga gcatacaacc gaaaacgctc ctcgtcagct tcactcacgt caaccacacg
     2101 atcggagtga tccagccgtt ggagcagctg ttaccgatca tcaaagagcg aaatcccaac
     2161 acgcggattc atatcgatgc ggcggtctcc ttgaacgcga tggatctcga tctcacgaag
     2221 cttccaatag actttttaac cttttccggt cacaagattt acggaccgaa agggataggc
     2281 gccgtcgttt tcaggaacaa cggactcctg aagccgacgc ttttcggttc ggtcacgacc
     2341 tctcctttta cccccggagc ggagaacata cccggtattg tggggttagc tgaagccctc
     2401 aggctagcgg ccgaaaaacg cgcgacttac cgcctcaaga cgcgccggtt gcaggagcgg
     2461 gcgataaacc tgatagaatc ggatataccg gatgtcgtcc tgaacgggcc caagccaacc
     2521 tcattcggga atctcgacgc catcccgaca caacgggcgg tggataatat caatttctct
     2581 ttccgttacg tggaggggga gtcgattatg ttgtttttag acttcgagga catcgtcgtg
     2641 gcgaccggaa gcgcttgtgc ctcctcggat ctcaaggtca actacgtcct cagcgcgatc
     2701 ggccgcgatc acgagatggc gcatggctcg cttcgcatca cgccagggtg ggacaacgat
     2761 gaggagcaga tcgatcggtt tgtcgatcga ttaaagccta tcgtgagtcg tttacgggcg
     2821 caaagcacct tgcggtagaa tcgccgaaac gtgaaatcct cgatcgaatc cgttcgaaag
     2881 aggtgttgaa aatagaaggc agacgcccaa aacagcccct agagtgaaca agctcgatag
     2941 aagatgaagg ggcgccgctt atgaaggagg aaaaaagcgt tggcagtcat caggtattca
     3001 gagaaagtat tggagcactt tcgaaatccg aaaaacatcg ggttgatcga gaacgcagac
     3061 ggtcagtcga ccgtcgggtc acccgcttgc ggggaccaag tttctatgtt cctaaaggtc
     3121 gaagaggacc gtatcgccga tatcaaattc ttgtcctacg gctgtgcttc gaacatcgcg
     3181 acgggatcca tcatcacgga gatggcgaag ggaaagacct tggccgaggc gaaagcgatt
     3241 acgtggaagg atgcggtgga cgagctggga ggtttaccac ccgtgaagat ccactgctcc
     3301 gtgttggcga tagacggatt aagggccgcc attcaggatt atgaggaaaa gacgaccggt
     3361 gtgaaatccg aaagaaaggt cgatgaaatg gcgattctgg atgagctgaa aaaggtcatc
     3421 tatcccaaga acggcatcaa catcgtggat acgaatatga tccgttacct ttctttcgaa
     3481 gaagggacca tctacatcga ggtttcactc gaggagacag acgtttttaa acaaaacgtt
     3541 ctggaagaaa tttacgaaca tctcgaagcc ttgcccggtg tggacaaggt cgagataaag
     3601 gttgtggaat actgatgaga gaagtctatt tagatcacgc ggcgacgact ccgccctatc
     3661 cagaggttct caaagcgcta caagaatcgt atgcgaccat ttacggaaac gcctcttctt
     3721 tacacgcgac gggccaaaaa gcgcgccgcg cactcgaaaa ggcgcgtgaa gacatcgcga
     3781 agctgatcga tgccgagcct gaagaggtct atatcacctc cgggggtacg gaatccgata
     3841 acatagcgct attcggaacc gaagcgctct accccggtca acctatgatc acctcgttga
     3901 tcgaacacga agccgtctta gagagcgcga aggcgttgga gtcgaaagga cacccggttc
     3961 gttttttacc ggtcgataca aatggagtcg tcgacttgaa cgcattgaag gcatatttag
     4021 aagaaaagcc ctttctggta tcggtgatgg cggcgaacaa cgaaatcggc acggtccagc
     4081 cggtcaagga gatcgcgaag ctcgcgcacg aaagaggggc gctcgtacat accgacgcgg
     4141 tacaagctgt cgggaagatt ccgattaatg ttcgtgagct gggggtggat atgctttctg
     4201 cttccgcgca caagttttat ggcccaaaag gcatcggatt cttctatttg cgcaaagggg
     4261 tgaaggtgct tccaatcgtt caaggcggcg gccacgaacg cgggttgcgt tccgggacgg
     4321 aaaatgtgcc gggtgctatc gctatggcgt tggcgctaaa aatatcgacc cgactgttca
     4381 ctcggacgca ctaccgatat gagcggtgga cggcccaaat cctgagtaaa tgcggagaaa
     4441 tcggcggttt tcgtttaaac ggacatcccc agcagcgggt ccccggtcta ttgagtttga
     4501 gttttaagag aatcaatggc gaagcgttga tggaactgct ggatatgaac ggcgtcgcgg
     4561 tttctacggc atccgcctgc caatctcact cagcaaaaag gggatactcg cacgtgattc
     4621 agagcctcgg gatcgatgaa gaatacgcca acgggacgat tcgtgtgtcg ttgggcgtgg
     4681 aaaacgtgca ggaagaggtc gatttcttct gtgaaatctt gaaaaaatgc gtgctgcagt
     4741 tgag
//
LOCUS       DAOPXN010000177         4711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_18280_length_4711_cov_4.853737, whole genome shotgun sequence.
ACCESSION   DAOPXN010000177 DAOPXN010000000
VERSION     DAOPXN010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18280_length_4711_cov_4.853737"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1522
                     /gene="metG"
                     /locus_tag="PKM49_08605"
     CDS             <1..1522
                     /gene="metG"
                     /locus_tag="PKM49_08605"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNY82892.1"
                     /translation="CPTCKREVRWVAEDNYFFRLSSFQEPLRELYEKNPSFVEPDFRR
                     NEMLSIIKGGLKDLSISRTSFSWGVPMPDDPKHVIYVWVDALINYISALGYATDNQEL
                     YRRYWPADLHLIGKEINRFHSIVWPAMLMAAGLPLPKKVFAHGWLTVNGEKISKSAGN
                     AIDPRLLMDVYGKDAVKYYLLRDINFGKDGDFSEDHLIQRINADLANDLGNLLHRTTA
                     MSLQNFAGVLEKEGGLNEETPDRDLIAAFETMKATYFEKMDRYQFTQALEALWAFIGF
                     SNKYIDMTEPWKLAKEESQRGRLRQVLRNLAEALRNIAIAIKPIMFSTSEIIYKRLGL
                     GSESWNEATFEDFTWDSARDFHMAEGEPLFPRRDIKEFKKVIQSPSLEPNNPTKDPSQ
                     EKPENLLIIDYDQFRTVQMKVGLVLEAKKVEKSRKLMRLSVELGEPKPRQIIAGIAQY
                     YEAEALVGKRIIVVTNLEPAKLMGMESFGMLLAAKDGVNLRLLTVDGEIQPGAAIS"
     gene            1519..4167
                     /gene="gyrA"
                     /locus_tag="PKM49_08610"
     CDS             1519..4167
                     /gene="gyrA"
                     /locus_tag="PKM49_08610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNY82893.1"
                     /translation="MKELVDKNGTEHDRSVVIERPFEEELSEAYINYSMSVIIGRAIP
                     DVRDGLKPVQRKLLYGMLELNLAHNQAYKKSARIVGEVMGKFHPHGDASLYETLVRMA
                     QDFNMRHPLVDGQGNFGSVDRDPPAAMRYTEARLTKIAEEMLADLDKDTVPFVPNFDE
                     SLKEPSVLPSNVPNLLMNGATGIAVGMATNIPPHNLGELVDAMAYLIDRPEATVQDLL
                     QFVKGPDFPTGGRVLRAENLRSIYETGSGSFTIEGVYSVEDDQIIIHEIPYGVNKAGL
                     IEQIAEYFKKTEKRMIRDIRDESDRNGMRIVIEVARGISPQVVMNHILKRSSIRVNFP
                     VKLLVIDAKMKPRVMNLKELSEAYLNHRYAVITRKTENELFVASRRAHIIEGLVKAVR
                     SIDTTIDIIRHSSDVPQATKNLMETLEITEEQAKAILDLRFARLTSIETNNLREELKN
                     LNETIARLKTLLSDPQNIRNEVKRLLLETKNKYGQPRLTKVGGGLPSEVDIEDLVVDR
                     PIVITVTKRGYILVSELQNYRTQARGGRGAKGIATREEDFARHIMVTTKLANTLFVSS
                     MGKVYTLKNYEIEDSTKGTRGKHLMSYLRLVEGEAIRTVLKMGIEFSEDTDLLFITAK
                     GKIKRTSLSDFSRAGTSGVIGLKLEEEDKVVDAVLAPAQDDHWNVFIGTAKGMAIRFP
                     LTSIRRMGRAAIGLKGISLKPDDLVVSMTLIHEKATDEPVLTVTTRGFGKRTPIASYR
                     IQSRGGIGLKNLAWIPELGDVLTVQVINDADEVIIVTKMGYTIRFSASDVRTMGRVTR
                     GVKVVDLGLEDEVASVERIQIDEEPSPAPKSGDKTPSKKAASPVDDISEVIEDIEDLE
                     EIDNLEESAEGAEADAESDHLDSENP"
     gene            4188..4526
                     /locus_tag="PKM49_08615"
     CDS             4188..4526
                     /locus_tag="PKM49_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY82894.1"
                     /translation="MLKVNEENGWSIVHMQDELDMSNSNQFKEEVIRVLIENGKTHVA
                     LDFSSLEYIDSSGLGTVVSLHKRCKMNGGKLVIFGISQTLLKLFKLTSLDKALNLYEH
                     KEAVFGSGKQ"
     gene            4523..>4711
                     /locus_tag="PKM49_08620"
     CDS             4523..>4711
                     /locus_tag="PKM49_08620"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNY82895.1"
                     /translation="MNLAIGADHAGFHLKEAIRQYFERREHYFVDCGTFDPFERVDYP
                     DYAKKVCEAVLSHEASFGI"
BASE COUNT         1277 a         1170 c         1284 g          980 t
ORIGIN      
        1 ctgcccaacc tgcaaacgag aagtgcgctg ggtcgccgaa gacaattact tcttccggct
       61 ttcctcattt caagaaccgc ttcgagagct ctatgaaaag aacccgtcct tcgtcgaacc
      121 cgacttccgc cgtaacgaaa tgttgagcat catcaaaggt gggctcaaag acctcagcat
      181 ttcccgcacc tccttttcgt ggggagtccc gatgccggat gacccaaaac acgtcatcta
      241 cgtctgggtg gacgcgttaa tcaactacat cagcgccctc ggttacgcca cggacaatca
      301 ggagctgtat cgtcggtatt ggccggccga tctacatttg ataggcaaag agatcaaccg
      361 attccactcg atcgtttggc ccgcgatgtt gatggccgcg ggtttgcctc tacctaagaa
      421 ggttttcgcg cacggttggc tgacggtaaa cggggaaaag atcagcaaat ccgcgggaaa
      481 tgcgatagac cccagactcc tgatggacgt ttacggcaaa gacgccgtta aatactactt
      541 gctcagagac atcaattttg gcaaggacgg cgacttttcc gaagaccatc tcatccagcg
      601 catcaacgct gacctggcga atgacctggg taacctgctg caccgcacga cggcgatgag
      661 tctacagaat ttcgctggcg tattagagaa agagggcggg ttgaacgagg aaaccccgga
      721 tagagacctc attgcggctt tcgaaacaat gaaagccacc tacttcgaga agatggacag
      781 gtaccagttc acacaagcct tagaagccct ttgggcgttc attggttttt caaataaata
      841 catagatatg accgaacctt ggaagttggc gaaagaggag tcacagaggg gaagactccg
      901 acaggttttg cgaaacctcg ccgaggcgct aaggaacatc gccatcgcca tcaaaccgat
      961 tatgttctcc acctctgaaa tcatctacaa acgtttaggg ctcggttcgg aatcttggaa
     1021 tgaagcgacc ttcgaagact tcacctggga cagcgctcga gacttccata tggctgaagg
     1081 ggaacccttg ttccctagaa gggatatcaa ggagttcaaa aaggtcattc aatcgcccag
     1141 cttggaaccg aacaacccga cgaaggaccc gtcgcaagag aaaccggaaa acttgctcat
     1201 catcgattac gatcagtttc ggacggttca gatgaaggta ggattggttt tggaagccaa
     1261 aaaggtcgag aaatccagga aattgatgcg gttatcggtc gagcttggag aacccaaacc
     1321 ccgacagatc attgccggta tcgctcagta ttacgaggcc gaagcgctcg ttggaaagcg
     1381 gatcatcgtc gtcacgaact tggaaccggc aaaactcatg ggaatggagt cttttggcat
     1441 gcttctcgcc gcaaaagatg gggtgaacct acggttgctg acggttgacg gcgaaatcca
     1501 acccggcgca gccatatcat gaaagaactc gtagataaaa acggcaccga acacgaccgc
     1561 agtgtcgtta tagagcggcc tttcgaagaa gagctgagcg aagcctacat aaactattcg
     1621 atgtcggtca tcatcggaag ggccattccg gatgtccgcg acgggctgaa acccgttcaa
     1681 cgcaagctgt tgtatgggat gttggagcta aacctcgctc ataatcaagc ctacaaaaaa
     1741 agcgcccgta tcgtcggaga ggtaatgggg aagttccacc cccacggaga cgcctctctc
     1801 tatgagacgc tcgtacggat ggcccaagat ttcaatatgc gtcacccgct ggtcgacgga
     1861 caaggaaact tcgggtccgt ggaccgtgat ccccccgcgg caatgcgtta tacagaggcg
     1921 cggctgacga aaattgctga ggaaatgctg gccgatctag ataaggatac cgtgccgttc
     1981 gttcccaact tcgacgaatc cttgaaggaa ccatctgtct tgccttcgaa cgtaccgaat
     2041 ctgcttatga acggcgctac aggaatcgcg gtagggatgg caacgaacat cccgccccat
     2101 aacctagggg aactcgtgga cgctatggcc tatctcatcg accgaccgga ggccacggtt
     2161 caagacctgc ttcagttcgt aaagggacct gattttccga ccggaggtag ggtcttgagg
     2221 gctgaaaacc tgcggtctat ctacgagacc ggttcgggct ctttcacgat agaaggggta
     2281 tactcggtcg aagacgacca aatcatcata cacgaaattc cctacggcgt caacaaagcc
     2341 ggactgatcg agcagattgc cgaatatttc aagaaaacag aaaagagaat gatccgcgat
     2401 atacgggacg aatcagaccg caacgggatg cgtattgtca tcgaggtggc gcggggtatc
     2461 agtcctcaag tcgttatgaa tcacattttg aaacgctctt cgattcgcgt caactttccc
     2521 gtgaagctcc tggtgatcga cgcgaagatg aaaccccgtg tgatgaacct gaaggaactc
     2581 tctgaagcct acctaaacca ccggtacgcg gtcatcacca ggaaaacgga gaacgagctg
     2641 ttcgtggcga gtcgtcgcgc ccatatcatc gaagggttgg ttaaagccgt aaggtcgatc
     2701 gacaccacga tcgatatcat ccgtcactct tccgatgtgc cccaagcaac caagaatttg
     2761 atggagacgt tggagatcac cgaagagcaa gcgaaagcga tactggacct ccggttcgcc
     2821 cgtttgacgt ccatcgaaac gaacaacctc agagaagaat tgaaaaacct gaacgagacg
     2881 atcgcccgct taaagacttt gctttcggac ccgcagaaca tccggaacga ggtgaaacgc
     2941 ctcctgttgg aaaccaaaaa taaatacggc caaccgcgct tgacgaaagt cgggggaggg
     3001 ctaccgagcg aagtggatat cgaagacttg gtcgtcgatc ggcctatcgt gatcaccgtc
     3061 acaaaaagag ggtacattct cgtttcggaa ttgcagaact accggacaca ggcgaggggc
     3121 ggccgaggcg ccaaaggaat agcgaccaga gaagaggact tcgccagaca catcatggtc
     3181 actaccaagc tggcgaacac gctgttcgtc tcctctatgg gaaaggtcta tacgctgaag
     3241 aactacgaga tagaggactc cacaaaaggc acgaggggaa aacatctgat gtcgtatcta
     3301 cgcttggtag aaggcgaagc gatccggacg gttctgaaga tggggattga gttttccgaa
     3361 gacaccgacc tgttgttcat taccgcgaaa ggtaagatca agcggacctc cctaagcgat
     3421 ttttcgaggg cggggacctc aggggtcatc gggttgaaac tcgaagaaga ggacaaggtg
     3481 gtggacgccg tgctggcccc cgcacaagac gatcattgga acgttttcat cggcacggcg
     3541 aaagggatgg ctattcgctt cccgctcact tctatccgaa gaatgggccg ggcggcgatc
     3601 gggttgaaag gaatatcgtt gaagccggac gacctcgtgg tgtctatgac gcttatccac
     3661 gagaaagcca cagacgagcc ggttttaacg gtgacgacgc gaggttttgg aaagcgaacc
     3721 ccgatcgcca gttaccgcat ccaatctcgc ggcggaatcg gcttgaagaa cctcgcgtgg
     3781 atccccgaat tgggcgatgt gctcactgtc caagtgatca acgatgccga tgaggtcatc
     3841 atcgtcacca aaatgggata cacgatccgc ttctccgcct ccgatgtccg gacgatggga
     3901 cgcgtcactc gcggtgttaa ggttgtcgat ttggggctcg aggatgaggt cgcttcggtg
     3961 gagagaatcc agatagacga ggaacctagc cccgctccga agtcaggtga taagacacct
     4021 tcgaaaaaag cggcgagccc ggttgatgat ataagtgaag ttatcgaaga tatagaagat
     4081 ttggaagaga tagataatct cgaagaatcg gcggaagggg cggaagccga cgccgagtct
     4141 gatcatttag atagcgaaaa tccatagatt cctaggaggg aacgaagatg ctgaaggtta
     4201 acgaagagaa cggttggtcc atcgttcata tgcaagatga gctggatatg tccaactcga
     4261 atcagtttaa agaagaagtg atccgcgtct tgatcgaaaa tggaaaaacc cacgtcgcgt
     4321 tggacttttc atccttggag tatatcgaca gctccggact cggcaccgtc gtcagcttgc
     4381 acaaacggtg taagatgaac ggcgggaagc tggtgatctt cggaatcagc caaactctct
     4441 taaaactgtt caaactcact tccctagaca aagccctgaa tctgtatgag cataaggaag
     4501 cggtctttgg aagcgggaaa caatgaatct ggctatagga gctgatcacg cagggtttca
     4561 tctgaaagaa gcgattcgac agtattttga acgtagagaa cactattttg tcgattgcgg
     4621 gacctttgac ccgttcgagc gcgtggatta tcccgactat gcgaaaaagg tgtgcgaagc
     4681 ggtcctgtct cacgaggctt cttttgggat t
//
LOCUS       DAOPXN010000178         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_18521_length_4662_cov_5.244194, whole genome shotgun sequence.
ACCESSION   DAOPXN010000178 DAOPXN010000000
VERSION     DAOPXN010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18521_length_4662_cov_5.244194"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2387
                     /locus_tag="PKM49_08625"
     CDS             <1..2387
                     /locus_tag="PKM49_08625"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNY82896.1"
                     /translation="KKMDLLLATMNLLDLERDPNLRDTKDHLGNKRVRTVGELLQSEF
                     ERGFVKIQKLIQEKLTIYNTLDKVSVQSLINVRPIIIAMNQFFASSQLSQFMDQVNPL
                     SELTNKRRLSSIGPGGLKRERAKIEVRDVHDSHYGRMCPIETPEGANIGLITSLSTFA
                     TIDRFGFLITPYSKVEKGKVTEEIVFLTADQEESYKIAQANTPVAPDGTIMTDRVVVR
                     FHGDIRYEKKEDVQFKDVSPKQMISISTALIPFLEHDDSNRALMGSNMQRQGVPLLDP
                     QAPRVGTGVEWVAARDSGYVVLAKYPGQVSKVDAKVIEIHRADANDRLIYEKKQPVID
                     VYRLAKFQRSNQDTLINQHAIVKAGDWVKTGDPIADGPATDMGELALGKNILVAFMPW
                     EGYNFEDAILVNENLLARDTFTSIHVEMYEVNSRETRLGAEEITPDIPNVSKERLKNL
                     DENGVVRVGAFVSSEDILVGKVTPKGEAEISPEEKIIRSVFGDRGRDIQDTSLKVPHG
                     VSGRVIDVKVYNKSEVAELGPGVNQMIRVYVACRKPLDIGDKLAGRHGNKGVVSKILP
                     CEEMPFLPDGMPVDMVLSPLGVPSRMNLGQILETHLGWLSWLKNVYVATPVFDGAKEN
                     EVLEELFSLRQKQKIETGDEKKDPTGKVLLRDGRSGLTFDQPIVVGIMYMMKLVHIAQ
                     DKIHARSVGPYSLIHQQPLGGKAQFGGQRFGEMEVWALEAFGATHTLNEMLTIKSDDV
                     VGRNEVYKAILKNKNIPETGVPESFKVLIKELRGVVLDTKVFDENGNEINLDRD"
     gene            2406..>4662
                     /locus_tag="PKM49_08630"
     CDS             2406..>4662
                     /locus_tag="PKM49_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNY82897.1"
                     /translation="MKRVKTVQITMASPDTVFGWSKGEVKKPETINYRTFKPERDGLF
                     CEKIFGPTKDYECSCGKYKGKKYEGTVCERCNVRVEPKSSRRKNMGHIQLAAPVVHLW
                     FLKSTPSILSNLLNMTSKNLENIIYFGSRRIIEKIFVIVDRKDTAFDNGDTLYETEHD
                     IYSLFWDFEAEPAVTVKNPTGPVKSEIQGMVSITEEETHTGKTLYWVTVTDKVSEPYA
                     VHKNRTINFKGGQEIQAGQQLVSEQTIPAIYSPIDGTVELDEGLGTLTIDPIITSGDQ
                     PVNFQIPFNARVTVKDNDKVKKGDRLTLEVTYPAILAEKSGTVVFDKGLSVKPLPDGR
                     HEATSNGKVLIENIIEQKRYPIVEGSILYVNEGEMVEKDAHIADRFVYEEEILSLTEY
                     RILEEHYPGMFNAEGEIENDRPIMVITEIDPEVSTEIEKEAGDILTDNEYEAYRTVYP
                     GKIEARTGAEAVKILLTKLDLEKIRVEIENELRELPKSSARVIKLRKRLQIIKDLLLS
                     GNDPTWMVLNVLPVIPPELRPMIQIEGGRFATTDLNDLYRRVINRNNRLEKLMKLNAP
                     EVIVRNEKRMLQQAVDALIYNGRMSKAITDRGGRPLKSLTDLLKGKKGRFRRNLLGKR
                     VDYSGRAVIVVGPDLKIHECGVPKKMALELFKPFVLSKLLGDETTSKSARKLKKAIIE
                     KEMPQAWEVLEEVIREHPVLLNRAPTLHRISIQAFMPRLVEGNAIRLHPLVCPPFNAD
                     FDGDQMAVHVPL"
BASE COUNT         1289 a         1190 c         1284 g          899 t
ORIGIN      
        1 tcaaaaaaat ggacttgctc ctcgcgacga tgaacttgct cgacctcgag agggacccca
       61 acctgaggga caccaaagac cacttaggca acaaacgcgt gcgaaccgtg ggcgaactcc
      121 tccaatcgga gttcgagcgc ggcttcgtca aaatacagaa attgatacaa gagaagctga
      181 ccatctacaa cacgctggat aaggtctccg ttcagagttt gatcaacgtc cggccgatca
      241 tcatcgcgat gaaccaattt ttcgccagca gccagctatc ccagtttatg gatcaggtga
      301 accctctctc agaactaacg aacaagagaa ggctctcctc gatcggacca ggcgggctga
      361 agcgggaaag ggcgaaaatc gaagtccgag acgtccatga ctcccactac gggagaatgt
      421 gcccgataga gacgccggaa ggggccaaca tcgggttgat cacctctttg tccaccttcg
      481 ccacgatcga caggttcggc ttcctcatca ccccatacag caaagtggag aaaggaaagg
      541 tcaccgaaga gatcgtgttt ctcacggccg atcaagagga gtcctataaa atcgctcagg
      601 cgaacacgcc ggtcgcccca gacgggacga ttatgaccga ccgcgtcgtc gtgcgcttcc
      661 acggcgatat ccgttatgag aaaaaagagg atgtgcagtt caaagacgta tcgccgaaac
      721 agatgatttc tatatccacc gccctgattc cctttctaga acacgatgac tcgaaccggg
      781 cgttgatggg ttcgaatatg caacgtcagg gcgtgccact cctcgatccg caggcgcctc
      841 gagtgggtac cggcgtggaa tgggtggcgg ctagagactc cgggtacgtg gtgttggcga
      901 agtaccccgg acaggtgtcg aaggtcgacg cgaaggtgat agaaatacat cgggccgacg
      961 caaacgaccg attgatctac gaaaaaaagc aacccgtgat agacgtgtac cggctggcga
     1021 agttccagcg ttcaaatcag gacaccctca tcaaccaaca cgcgatcgtg aaagccggag
     1081 attgggttaa aaccggagac cccatagccg atgggccggc cacggatatg ggcgaattgg
     1141 ccttaggcaa gaacatcttg gtcgccttta tgccttggga agggtataac ttcgaagacg
     1201 cgatcctggt caacgaaaac ctgttggccc gagacacctt cacttccata cacgtggaga
     1261 tgtacgaagt caactcgcgt gagacccgtt taggcgcgga agagatcact ccggatatcc
     1321 cgaacgtcag caaagaacgg ttgaaaaacc tagacgaaaa cggtgtcgta cgggtagggg
     1381 catttgtctc ttccgaagat attttggtcg gcaaggtcac cccgaaaggg gaagcggaaa
     1441 tctctcccga ggagaagatc atccgttcgg tctttgggga tcgcggtcgc gatatccaag
     1501 acacctctct caaagtcccg cacggggttt ccggaagagt gatcgatgtg aaggtgtaca
     1561 acaaatccga ggtggctgaa ctgggccccg gtgtcaacca gatgatccgg gtctacgtcg
     1621 cctgccggaa accgttggac atcggagaca aattggccgg ccgtcacgga aacaaagggg
     1681 tcgtttcgaa gatattgcca tgcgaagaga tgccattctt gcccgatgga atgccggtgg
     1741 atatggttct tagccctttg ggtgtgccgt ctcgtatgaa tttggggcag attctggaga
     1801 cccacctcgg gtggctgagc tggctaaaaa acgtctacgt ggccacaccg gtcttcgacg
     1861 gggcgaaaga gaacgaagtc ctggaagaac ttttctcctt aaggcagaag cagaagatcg
     1921 agactggaga tgaaaaaaag gatccgacgg ggaaggtctt gctccgtgac ggtcgaagcg
     1981 gtttaacctt cgaccaacct atcgtcgtcg gtattatgta tatgatgaaa ttggtgcaca
     2041 tcgcccagga taagattcac gcccgaagcg ttgggcccta ctctttaatc caccagcagc
     2101 ctttgggtgg gaaagcccag ttcggtggcc agcgattcgg ggaaatggaa gtgtgggcgc
     2161 tcgaagcctt cggagcgaca cacaccctca atgaaatgct gaccatcaag tcggacgatg
     2221 tcgtgggcag aaacgaagtg tataaggcga tcttgaaaaa caagaacatt cccgaaacag
     2281 gcgttccgga aagttttaag gtgctgatta aagagttgcg cggcgtagtc ctggatacca
     2341 aagtcttcga tgaaaacggt aatgaaatta acctggatag ggattaaaag ggaagggatg
     2401 atttcatgaa aagggtaaaa accgttcaaa tcacgatggc ctctccggat acggttttcg
     2461 gttggtcgaa aggcgaggtc aaaaaaccgg aaaccatcaa ctaccggacg ttcaaacccg
     2521 agagggacgg cctgttctgt gaaaagatct ttgggccgac caaagactac gagtgctctt
     2581 gcggcaagta caaaggaaaa aagtacgaag gaaccgtctg cgagcgctgc aacgtgcgcg
     2641 tagaacccaa gtcttctaga agaaagaata tggggcacat tcagctggcg gccccggtgg
     2701 tccatctctg gtttctgaag agcacaccaa gcattttgtc caaccttctg aatatgacca
     2761 gcaaaaactt ggagaatatc atttattttg gctccagaag gatcatagaa aagatatttg
     2821 tcattgtcga tagaaaagac accgccttcg ataacggcga caccttatac gagaccgagc
     2881 acgatatcta cagcctgttc tgggacttcg aagccgaacc ggcggtcacc gtgaaaaacc
     2941 ccaccggacc ggtgaaatcg gagattcagg gaatggtttc cataaccgaa gaggaaaccc
     3001 ataccggcaa aactctgtac tgggtgaccg tgacggataa ggtctcagaa ccctacgccg
     3061 ttcacaaaaa ccggaccatc aacttcaagg gaggccagga gatccaagcc ggacaacagc
     3121 tcgtgagcga gcagaccatt ccggcgatct actccccgat cgacggaacg gtagaactgg
     3181 acgaaggctt ggggactttg accatagacc cgatcatcac gagcggcgac caacccgtga
     3241 acttccaaat ccctttcaac gcgagggtca ccgttaaaga caacgacaaa gtgaaaaagg
     3301 gagaccgtct gaccctggaa gtgacctacc ctgcgatcct ggcggaaaaa tccggaaccg
     3361 tcgtgttcga caaagggcta tcggttaagc ctttgccaga tggtcgacac gaagcgactt
     3421 ccaatggcaa ggtgctcatc gagaacatca tcgaacaaaa acggtatccg atcgtcgaag
     3481 gctctattct ctacgtcaat gaaggggaga tggtggagaa agacgcgcat atcgccgatc
     3541 ggttcgtcta tgaagaggag attctctcgc tcaccgaata ccgcatccta gaagagcatt
     3601 accccgggat gttcaacgcc gaaggcgaaa tcgaaaacga cagaccgatt atggttatca
     3661 cggagattga ccctgaggtc tcgacggaga tcgaaaaaga agccggcgat atcctcaccg
     3721 acaacgaata cgaagcctac cggacggttt accctggaaa gatcgaggcc aggaccggcg
     3781 cggaggccgt gaagatcctt ctgaccaaac tggatctgga aaagatacgc gtagagatag
     3841 agaacgaatt gcgagagctt ccgaaatcca gcgcgcgggt gatcaaactc agaaaacggc
     3901 tgcaaatcat caaagacctc ttgctatcag gaaacgatcc cacctggatg gtcttgaacg
     3961 tcttgcccgt catcccgccg gagcttcgcc cgatgatcca gatcgaagga ggacggttcg
     4021 cgacaaccga tttgaacgac ctgtaccgcc gggtcatcaa ccgcaacaat cggttggaaa
     4081 aattgatgaa gttgaacgcc ccggaagtga tcgtgcggaa cgaaaaacgg atgctgcagc
     4141 aggcggtcga cgcgctcatc tacaacggga ggatgtccaa agcgatcacc gatcgaggag
     4201 gtcgcccctt aaaatcttta accgatctgc tcaagggaaa gaaaggccgg ttccgcagaa
     4261 accttctcgg gaaacgggtg gattactcag gacgagcggt catcgtcgtc ggcccggact
     4321 tgaagataca cgaatgtggg gtccccaaga agatggcgct cgagctgttc aaacccttcg
     4381 tcctctccaa attgctcggg gacgaaacca cttccaagag cgctcgtaag ttaaaaaagg
     4441 caatcataga aaaagagatg cctcaggcgt gggaggtgct cgaagaggtg atccgggagc
     4501 atccagtctt actcaaccgc gcgccgacat tgcacagaat ctcgatacag gcctttatgc
     4561 ctcgattggt cgaagggaac gccattcggc tgcatccctt ggtctgtccg cctttcaacg
     4621 cggacttcga cggagaccag atggcggttc acgtacccct gt
//
LOCUS       DAOPXN010000179         4615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_18773_length_4615_cov_4.913158, whole genome shotgun sequence.
ACCESSION   DAOPXN010000179 DAOPXN010000000
VERSION     DAOPXN010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18773_length_4615_cov_4.913158"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKM49_08635"
     CDS             complement(<1..248)
                     /locus_tag="PKM49_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82898.1"
                     /translation="MMKKTLIFILCLLIVLWFCGPVLLILIAALTPSAEYYDLARLVP
                     TKLTLTHLESLFVRLNGWKATLISIQTALLSIVISFAIG"
     gene            complement(245..1099)
                     /locus_tag="PKM49_08640"
     CDS             complement(245..1099)
                     /locus_tag="PKM49_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82899.1"
                     /translation="MIHHKGKIALMLLLPTFAYLIVFIFYPLVDTCLLAFSNPAGPLE
                     NFRRIITSGEFWKTLRNTLVITGIVVPLQLILGLALALFVNLRFKGYLFILYIIAIPL
                     ALSDVSAALMSYSIFASTGFLNKLLMAWGWIERPLYFFGFRFQDRAFWVIVITEVWRA
                     TPLVFIILLAGLQAVNKEYLEAANVFGFSPWQKLWKIVLPILRPSILSALLIRTIFAF
                     QVFGVIWILAGRDIPVLAGEAYYWQTVVYNSNISSAYGLIIAVVTILISWAYITFLKP
                     KSFEEEAV"
     gene            complement(1193..2467)
                     /locus_tag="PKM49_08645"
     CDS             complement(1193..2467)
                     /locus_tag="PKM49_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY82900.1"
                     /translation="MKRTLLFGLCLSLLLAVAFGQLNFVSTQFHPATEREYFEGSLLP
                     DFVKLTNVKVQFLPLTYEEASVRLRAEQAANRVTIGLFAELQGGMELMASGGLLKDIS
                     GVTFSDRTFISTFEKYAHGYGIKQFVPWLQATFVMVINKKAFDYLPKGLTKEDVLKGT
                     SKWTYQALLEWAKNIYDKTKMPMVGFPENPQGLFHRFLHGYLYPSFTGGQFVKFDSLD
                     AVDMWAYLKELFKYVSPGSSTWSFMSEPLMRNEAWIVWDHTARIPAAVRADPEQYIPA
                     PVPAGPKGRGFITVLVGLALPKNAPNEAEAVRLIDYLTSATTQFDILEKTGFFPVIEE
                     AIGVIPAGPNKVLAEAVVAQAGAPDAMVSFIPGLGPKGGEFTAYYREAFRRIVYENED
                     IKKVLQDIAPKVYQTVKESGATLPQPDASLFH"
     gene            complement(2498..3337)
                     /locus_tag="PKM49_08650"
     CDS             complement(2498..3337)
                     /locus_tag="PKM49_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HNY82901.1"
                     /translation="MDSILLKAKSLYEALKPSEKKVANYLIQRADDVIHFSITDLAEN
                     AGCSEASISRFVRKLGFSKFIDFKISLVKELSLLEDKQNVKNDATGVADLIHHLVQQG
                     VKSYKELSQLVNPLDIEKAADWIAASKHTFLFGVGSSGSIAELGASILVRIGVSCFAY
                     ADPHMQVIVGSNCTPEDTVIGVSVSGTIKDTIKSVQVAKNVGAKTIAITGGKESPLSK
                     EVDLVLYSSPQGFEAGIGSLGARSSQMAILDMLISTIVWKNETCRNTIEKVEQALVPK
                     RFS"
     gene            3782..>4615
                     /locus_tag="PKM49_08655"
     CDS             3782..>4615
                     /locus_tag="PKM49_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HNY82902.1"
                     /translation="MKIGLMHFRSGFTDGVSLEMDKQKTVLEEMGHEVVFVCGELQSG
                     EGIEIPLLHLLEPRNERLSRNGFGSLEDFTTEEYEEELNDLAYLIESQVTRVILEHQI
                     EMLIVHNLFSLGLNLAAAKGLSRAIEKTEIRAMAHHHDFYWERAKFSRPTLSVVNRFL
                     SSFFPPNSKNVTHGVINSLAQKELLRKRNLSSVIIPNVMDFNQPPWLLDEFNNDLRER
                     LGIGANDLVFLQATRILPRKAIELAFEFIRVFYEEQFPKVQGGTRLYTDRKIDGKSAL
                     HF"
BASE COUNT         1185 a         1123 c         1209 g         1098 t
ORIGIN      
        1 ccgatcgcaa aactgatcac gatggagagt aaagctgtct gaatgctgat gagggttgcc
       61 ttccaaccgt tcaatcgcac gaaaaggctc tccaaatgcg ttaacgtcag cttggtcggg
      121 accagccgcg ccaagtcata gtactcggcg gacggggtga gcgcggcgat gaggatcaac
      181 agaacgggcc cacagaacca gaggactatg agtaggcaaa ggatgaagat tagagttttt
      241 ttcatcatac cgcttcctcc tcgaagctct tgggtttgag gaaggtgatg taggcccagc
      301 tgatcaggat cgtcactacg gcgatgatca gaccgtaagc gctggatata ttcgagttat
      361 agacgaccgt ttgccaataa tacgcttctc ctgcgagaac cgggatgtct cttcccgcga
      421 gtatccagat gacgccgaat acttgaaaag cgaagatcgt ccggatgagc agcgccgata
      481 gaatagaagg ccgaaggatt ggaagcacga tcttccacaa cttttgccaa ggagaaaaac
      541 cgaaaacgtt cgcggcctcg aggtactctt tattaacagc ttgaagtccc gcgagcaaga
      601 tgatgaagac caaaggtgtg gcgcgccata cctctgtaat gacgataacc cagaaagcgc
      661 gatcctggaa gcgaaaccca aaaaagtata agggtcgctc gatccatccc caagccataa
      721 ggagcttgtt cagaaacccg gtagaggcaa agatggaata gctcataagc gcggcagaca
      781 catcgcttaa ggcaagcggt atcgcgatga tatataggat gaataggtat cctttgaatc
      841 gcaggttgac gaatagggcc aaagccaagc cgaggatcag ttgtaggggt acaacaatac
      901 cggtaatgac caaagtgttg cgcagcgtct tccagaattc tccggaagtg atgatccggc
      961 gaaagttttc tagggggccc gctgggttcg aaaaggctaa cagacaggtg tctaccaacg
     1021 gataaaaaat gaagacgatc aggtaggcga aggtcggtag aaggagcatt aacgcgatct
     1081 ttcccttgtg atgaatcata ctgtacccct ccgtgaagat cgtataggta taacgaaggt
     1141 agtaaaaagc gccggcgaga cgctataggt ttagccggcg ctcatatcga ttttagtgaa
     1201 acaaagacgc gtccggttga ggcagcgtgg ctccagattc tttcacggtt tggtagactt
     1261 tcggcgcgat gtcttgcagt acttttttaa tatcttcatt ctcgtagaca atccggcgga
     1321 acgcttcgcg atagtaagcg gtgaactctc caccttttgg cccgagaccg ggaatgaaag
     1381 aaaccatagc atccggagcc ccggcttgcg ccacaaccgc ttcagccaac actttattcg
     1441 gcccggccgg gatgaccccg atagcttctt cgatcacggg gaagaagccg gtcttttcca
     1501 atatgtcgaa ttgggtagtc gccgaggtga ggtagtcgat cagcctgact gcttccgctt
     1561 cgttcggggc gttctttgga agggccaaac ccactaatac cgtgatgaag cctctacctt
     1621 ttggacctgc cgggaccgga gccggaatgt actgttcggg gtccgcgcga accgcagctg
     1681 gaattcgagc ggtatggtcc cagacgatcc aagcttcgtt tctcattaaa ggctcactca
     1741 tgaagctcca agtggaacta ccggggctaa cgtatttgaa cagctctttc aagtaggccc
     1801 acatatcgac ggcatcgagg ctgtcgaact tgacgaactg tcctcccgtg aacgaggggt
     1861 aaaggtaacc gtggaggaag cgatgaaaca gtccttgcgg gttttcgggg aagccaacca
     1921 tcggcatttt ggtcttgtcg tagatgtttt tcgcccattc taagagggct tggtacgtcc
     1981 atttcgaggt gcctttgagt acatcttctt tcgtaaggcc tttcgggagg taatcaaagg
     2041 ctttcttgtt gatgaccatc acgaaggtcg cttgaagcca cgggacgaac tgcttgatcc
     2101 cgtatccgtg cgcatatttt tcaaaagtgg agatgaaggt acggtcagaa aaggtgacgc
     2161 cggagatatc tttgagtaaa ccacccgaag ccatcagttc catcccgcct tggagctcgg
     2221 cgaagaggcc aatcgtcact ctgttcgccg cttgctcggc gcgtaatcgc acactggcct
     2281 cttcataggt taggggaaga aactggacct tgacgtttgt cagcttaaca aaatcgggaa
     2341 gcaacgaccc ttcgaaatac tcccgttccg tagccgggtg aaactgagtt gagacgaaat
     2401 tcagctgtcc gaaagctacg gctagcagca aagaaagaca caacccaaac aacaatgttc
     2461 ttttcatagg ttccctcctt gtttcatgtt tcaaggttta cgaaaagcgc ttaggtacga
     2521 gcgcctgttc gactttttca atggtgttgc ggcaggtttc gttcttccag acaatggtgc
     2581 tgatgagcat gtcgaggatg gccatttgtg acgagcgcgc ccctaatgag ccgattccgg
     2641 cctcaaaccc ttgtggcgaa ctgtatagca cgaggtcgac ctctttcgag agcggagact
     2701 ctttaccacc agtgatcgcg atggtttttg cccccacgtt cttggccact tgtacggact
     2761 tgatcgtgtc tttgatcgtc ccggaaacgg agacgccgat tacggtgtct tccggtgtgc
     2821 aattggagcc gacgatcact tgcatgtgag ggtcggcgta ggcgaaacag gaaacaccga
     2881 tgcgaaccaa aatactggcg ccgagttcgg caatcgaccc cgaagacccg actccgaaaa
     2941 gaaaggtgtg cttcgatgcc gcaatccaat cggcagcttt ttcgatgtct agagggttaa
     3001 cgagctgcga caactccttg taagacttaa ccccttgttg gaccagatgg tgtatcagat
     3061 ccgcaacgcc cgtcgcgtcg tttttgacgt tctgtttgtc ttccaagaga gagagctcct
     3121 tgaccaaaga aatcttaaaa tcgatgaact tggaaaaacc cagttttctc acgaaccgtg
     3181 agattgaggc ttcgctgcac ccagcgttct ctgcgagatc ggtgatcgaa aaatgaatca
     3241 cgtcgtccgc gcgttgaatg aggtaattgg cgaccttctt ctcagaaggc ttcaacgctt
     3301 cgtatagact tttcgctttc agtaggatcg agtccatctt cttctccgtt tttgaaaata
     3361 tttttcaaga ataatatttt cacacaaatt atgaacgcga aaatagtata caccactttc
     3421 tactctttgt caaccacgat aatcacgaag gatgcaagag gacagtgtga gtcaagtttt
     3481 gagctgacgt agctaagagg ttcatcgcgg ggtaaccagc gggctgactt cgtaagcccg
     3541 cgaagtctcg cgatactcat tttgctttta atgacgccgt aattggcatg tacagaagcg
     3601 tatcgcgccg tcgcaagaag aaaggacgct tacgccgcga tgaccacgtg gacttaaaat
     3661 cgtccgagtg attttggtct tgtttcttga ttacacccca taacttgact cgcacgctca
     3721 taaactctgc tttcatttcg tgatagaata aagtggaggc aaaataagga gaggtgatcg
     3781 tatgaaaatc ggtttgatgc atttccgttc tgggtttacc gacggggttt cattggagat
     3841 ggacaaacag aaaaccgttc tcgaggaaat gggtcacgag gtggtcttcg tctgcggaga
     3901 gctccaaagc ggagaaggca tagaaatccc gttgcttcat ctattagaac cgcgcaacga
     3961 gcgcctatcc cgaaatggtt ttggttcgct ggaagacttc acgactgagg aatatgaaga
     4021 ggagctaaac gatctcgcgt atctgatcga gtcgcaagtc acccgtgtga tcctcgagca
     4081 tcagatagag atgctcatcg tccacaatct cttttcactt gggctgaatt tagccgcggc
     4141 aaagggcctt tcacgcgcga tcgagaagac ggagatacgc gcgatggccc atcaccatga
     4201 cttctactgg gagcgagcga aattttcgcg gcctactctc tcagtcgtca atcgctttct
     4261 aagctccttc ttccccccga acagcaaaaa cgtgactcac ggcgtcatca acagtctggc
     4321 tcagaaggag ctgctgagaa agcgcaacct ctccagtgtg atcattccga atgtgatgga
     4381 cttcaaccag ccgccttggc ttttggatga gttcaataac gacttacgcg aacggttggg
     4441 aataggcgcg aacgacctcg tgtttctaca agctacccgg atactcccaa gaaaggctat
     4501 agaactagcc ttcgaattta tccgcgtttt ttatgaggaa cagttcccta aggtccaggg
     4561 gggcacacgc ctatacacgg atcggaagat agacggtaaa agcgcgttgc atttc
//
LOCUS       DAOPXN010000180         4590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_18889_length_4590_cov_5.026020, whole genome shotgun sequence.
ACCESSION   DAOPXN010000180 DAOPXN010000000
VERSION     DAOPXN010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18889_length_4590_cov_5.026020"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3481)
                     /locus_tag="PKM49_08660"
     CDS             complement(<1..3481)
                     /locus_tag="PKM49_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNY82903.1"
                     /translation="MRRMIKLLPIFAVLLLIVSCVRTPLAIENDTISLIQKDDGVAIT
                     LKTTVLGVELTFSQKGPAVTPILEDRLLTIFDPDSTLFRYSFVNPNGPIEKGTELMVI
                     PGKRVADIAIKKVDVVRQLPEPRANPPFANGFSVKDQAVGISETFDLEIYGKNITNLG
                     GFELWIEYDPALLQVNSSGNPIALTGPCSGGLMLSPVYYAGQIKITAAFTGGVTIGSS
                     AVPVLRIRFQAKTSEGNAYVRFGSATVISNTSASPMTVGLQNGTVAITAGSGPILLGD
                     FNKDKIVNVLDFVAFANHYNTSASTSRAYNVLYDIAPAEDHYGGIWAGIYDTCTPDGK
                     INLLDFVIFGSNYGKEEPTQANENPSVPTGPTPANNSTGISTSPTLSWNPSTDPDGDP
                     VSYDVYMDTNQNPTTLKTTTSLTTPQYAASGLTTGTKYYWKVVAKDDKGGASTGGPWS
                     FTTQAQVNNPPSAPTNPTPASGTTGTGISLTLSWNASTDPDAGDVVSYDLYWGTSSNP
                     TSLKAGNLSSTSYAISSLSYSTKYYWKVVAKDNHGASTPGAVWNFTTGAAPSTAKYRA
                     LTLGLTIYDDGNNLDAPIYDSQDMAQMLSHLSEGYTTTVKTGRVTKSQLNTALTGYVS
                     GSQANDVFFFHYSGHGWYTQGQSNLYLSDGQSMSVSELRQKLSAINGTKIVIIDACES
                     GSFVNLIEGRELALEEKRERMRLFNENVLEVFSEPSSDRGTYTSPHEYYVLTGASIHQ
                     YSYEDDYLNNGFLSFFFLDGMGDVGVSNPNGAFDYTYNADGYGAGGVVDGKITFREIY
                     NYAKDKVQSYAAASGDTQTVQGTPTSSTYIVGSYSGGATNNPPTTPSNPTPSNGSTGI
                     SLTQTLSWTSSGATKYDVYFGTSSSPGKVSTNQTVALYNPGSLQEGKTYYWKIVAKND
                     YGSATGPVWSFTTLSGGSTGDGARVILRSPTPEIIGNIAFAYQNAFYDYQNPTPPHWK
                     AEVGGWLTFTFTGKTDFNFTAYGIPAGGSPAYIEGYVNGVYVGYGELARKWENWYIGE
                     EDMSTGQNVVKLQVFNGTMWIDEAWVDINGEPVQNPPSTPANPSPSNGASGVSPNPTL
                     TWSSLNATSYNVYFGTQSVPSLVQSGISSASYSPSGPLTAGNTYYWKVVAVNAYGSTP
                     GPVWSFTVQSGSGD"
     gene            complement(3494..4303)
                     /locus_tag="PKM49_08665"
     CDS             complement(3494..4303)
                     /locus_tag="PKM49_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HNY82904.1"
                     /translation="MKRKTVVSIFLLLVWLTVACSSGDGGGGGNGALPSLPQLLSPTR
                     TILTTLTPTFRWTSNFATSYDFYLGQIPDPPKVTSPSAQQYTPASNLEPGQLYYWRVV
                     GKNALGDSPSSVTSYFVTPEANPPGPALQIRDAVVTPNTQFFVTVKGWNLTGVMGIEL
                     TLEYNESELELVNIGGNYENTFQPMGPMSGSLLIGVVRPGSVTVTMSLIQDPVDIPNN
                     EILRVTFRSKNGSYPAKITIENTSSIIAKVGNNLITIDFDKSDQGHFLIGS"
BASE COUNT          987 a         1208 c         1255 g         1140 t
ORIGIN      
        1 catctccact gcccgattgg accgtgaagg accaaacggg tccgggagtg ctcccgtagg
       61 cgttgaccgc gaccaccttc caatagtagg tgttgccggc ggtcaagggg ccgctgggac
      121 tgtagctcgc actggatatt cctgattgaa ccaacgaggg cacgctttgg gttccgaaat
      181 agacgttgta ggaagtggcg ttcaaagagg accacgtcaa ggtggggttc gggctaaccc
      241 cggaggcgcc gttcgacggc gacggattcg ccggggtaga gggcgggttc tgcacgggtt
      301 cgccattgat gtccacccac gcttcatcga tccacatcgt accgttaaag acctggagct
      361 tcaccacgtt ctgtcccgtt gacatatcct cttcgcctat ataccagttt tcccatttgc
      421 gcgctaattc gccgtaaccc acatacactc cgttcacata gccctctatg tatgcgggag
      481 aaccgccggc aggaatgcca taagccgtaa aattgaagtc agtttttccg gtgaaagtga
      541 aggtcagcca tccgcccacc tccgctttcc aatgcggggg tgttgggttt tggtagtcat
      601 aaaaagcgtt ctggtaagcg aatgcgatgt tgccgatgat ctcgggagtc ggcgagcgta
      661 ggatcactct ggcgccgtcc cccgtcgatc ccccgcttag ggtcgtaaag gaccagacgg
      721 gcccggtggc gctgccgtag tcgttcttcg cgacgatctt ccaatagtaa gtctttccct
      781 cttgcaggct tccagggtta tacaaggcga cagtctggtt cgtgctgacc tttcctggtg
      841 aggacgacgt tccgaagtaa acgtcatatt tcgtggctcc ggaactcgtc cagctgagcg
      901 tttgcgtcaa actgatgcca gtggatccgt tcgacggagt gggattgctc ggtgtggtcg
      961 gcgggttgtt tgtcgctcct cccgaataac tccccacgat gtacgtcgaa ctcgtcgggg
     1021 ttccttgaac cgtttgcgta tcgccagaag cggccgcgta gctctgcact ttatctttcg
     1081 catagttgta gatttctcgg aaggtgatct tcccgtctac cacaccccct gcaccatacc
     1141 cgtccgcatt gtaagtatag tcgaatgcgc cattcgggtt cgacaccccc acatccccca
     1201 ttccgtcgag aaagaagaaa gatagaaagc cgttgttgag gtaatcgtcc tcataactgt
     1261 attgatggat cgatgcgccc gtgaggacgt agtactcatg aggagaggta taggtaccgc
     1321 gatccgatga gggttcgctg aagacttcca gcacgttctc gttgaacaac cgcatccgtt
     1381 cacgtttctc ttccagcgcc agttcgcgtc cttcgatcag attcacgaaa ctccccgatt
     1441 cgcaggcgtc gatgatgacg atcttcgtcc cgttaatggc tgacaacttt tgacgcaatt
     1501 ccgatacgga catcgattgc ccgtcggaaa ggtacaggtt ggattggcct tgggtgtacc
     1561 atccgtgacc ggagtagtgg aagaagaaga cgtcgttcgc ctgactgccg cttacatatc
     1621 cggtcaaagc tgtgttcagc tgggatttcg tgacccttcc cgtcttcacg gtggtcgtgt
     1681 aaccttccga taagtgcgaa agcatttgag ccatatcttg cgagtcgtag atcggggcgt
     1741 ccagattgtt cccatcgtca tagatagtga gcccgagggt tagcgcccga tatttcgctg
     1801 tgctgggcgc cgctcccgtc gtaaaattcc ataccgcccc aggagtgctc gcgccgtgat
     1861 tgtctttggc gacgaccttc cagtagtact tcgtgctata actgagactc gaaatcgcat
     1921 aactcgtgga ggagaggttt cccgctttta gcgaggtggg gttggaggag gtaccccagt
     1981 acagatcgta ggatacgacg tcccctgcgt ccggatcggt ggaggcgttc cacgagagtg
     2041 tcaagcttat gcctgttcct gtggtaccgc tggccggtgt gggattggtc ggcgcgcttg
     2101 gcgggttgtt gacttgcgcc tgggtggtga aactccacgg cccgcccgtg ctggcgcccc
     2161 ctttatcgtc cttcgcaacc accttccaat aatacttcgt gccggttgtg agcccgctgg
     2221 ccgcgtattg tggtgtggtc aggctcgtag tcgttttcaa tgtggtcgga ttctgattgg
     2281 tgtccatata tacgtcgtaa gaaaccggat cgccatcggg atccgtactc ggattccaag
     2341 acagggtcgg ggaggtagaa atgcctgtgg agttgttcgc cggcgtcggc cccgtcggaa
     2401 ctgacgggtt ttcattcgct tgcgtcggct cctccttccc atagttgctc ccaaaaatga
     2461 cgaaatcgag gaggttgatc ttgccatcgg gtgtgcaggt atcgtaaatg ccggcccaaa
     2521 ttccaccgta gtggtcctcg gccggagcga tgtcatacag cacgttgtag gctctggatg
     2581 ttgacgctga agtgttgtag tgattggcaa aggcaacaaa atccaacacg ttcacgattt
     2641 tgtctttgtt gaaatctccc agcaagatag gcccacttcc ggcggttatg gcgacggtgc
     2701 cgttttggag accgaccgtc atcggagaag cgctggtgtt gctgatcacg gtcgcggatc
     2761 caaatcggac atacgcgttg ccttcgctcg ttttggcctg aaatcgtata cgaagaacag
     2821 gtactgccga actgcctatc gtgacgccgc cggtgaatgc ggcggtgatc ttaatttgcc
     2881 ctgcgtaata cacgggcgaa agcataagac ccccactgca agggccagtt aaggcgatgg
     2941 ggttgccgga gctgttaacc tgcaaaagcg cgggatcgta ttcgatccat aactcgaaac
     3001 cacctaaatt cgtgatattc ttcccgtata tttccaagtc aaaggtttca gaaatgccga
     3061 cggcttggtc tttgacggag aagccgttcg caaacggtgg attcgcccgt ggctcgggca
     3121 attgcctcac gacatcgacc ttttttattg cgatatccgc aactctcttc cccgggatca
     3181 ccatcaattc ggtacctttt tcgatcggtc cgttcggatt gacgaaagag taccgaaaca
     3241 gcgtggagtc ggggtcgaat atcgtcaaga gtcgatcttc caagatgggc gtaacggcag
     3301 gccctttctg tgaaaaagtc aattccacgc ccaggacagt ggttttgagg gtgatcgcga
     3361 ctccatcatc cttttgaatc agcgagattg tatcgttttc tatggccaag ggcgtgcgaa
     3421 cacaagagac aattaacagc agaacggcga aaatcggtag cagttttatc attcgacgca
     3481 tcgttatccc tccttacgat ccgattaaaa aatgaccctg gtcgctcttg tcgaagtcga
     3541 tggttatcaa gttgtttcct acttttgcaa tgatactcga ggtattctct atcgtaattt
     3601 tcgccggata ggagccgttc ttggaacgaa aggtcacacg cagaatctca ttgttcggta
     3661 tatcgacggg gtcttgaatt aacgacatcg tcaccgtcac gctacctggc ctaacaacgc
     3721 cgatcaaaag agatcctgac atcggtccca taggctgaaa tgtattctcg taatttcccc
     3781 cgatatttac cagttccagc tccgattcgt tgtattcgag ggtgagctcg atccccataa
     3841 cccccgtcag gttccagcct ttgaccgtta caaaaaactg tgtgttgggt gtgacaacgg
     3901 catcccttat ttggagcgcg ggaccggggg gattggcctc cggtgtcaca aagtaactgg
     3961 tcacgctcga tggggagtcc cccaaggcgt tttttccaac cacccgccaa tagtacaact
     4021 ggccgggttc caggttgctg gccggtgtat attgctgcgc gcttggcgag gtgactttcg
     4081 gaggatccgg gatttggccc aaatagaaat cgtatgaggt cgcgaaattc gaggtccacc
     4141 taaaggtggg ggttagagtc gttaaaatcg tcctagtcgg actcaataac tggggcaggg
     4201 aaggtaacgc gccgttccct ccgccaccac cgtcccctga actgcatgcg accgttaacc
     4261 aaaccaataa taggaaaatc gaaacgactg tttttctttt catcgatagc caactccttc
     4321 actataacgc gaaatgaaaa aacccggtga aaactcgcga tacccgcgag gttagccttt
     4381 ctttatagac gaagaattat atcataacct ttccttagaa aaaggtgaga ggtgtgggtc
     4441 aagttatggg gtgatataac taagaggttc atcgcggggt aaccccacac aggcatcgcg
     4501 gcgtaagcgc cccttctctt tgcgacggcg cgataccctc tttagaaggg cgaagaaggg
     4561 atcgcggggt cccttcggtt accccgcgat
//
LOCUS       DAOPXN010000181         4561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19042_length_4561_cov_4.920550, whole genome shotgun sequence.
ACCESSION   DAOPXN010000181 DAOPXN010000000
VERSION     DAOPXN010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19042_length_4561_cov_4.920550"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PKM49_08670"
     CDS             complement(<1..264)
                     /locus_tag="PKM49_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82905.1"
                     /translation="MKIFSFILRNRWLFILLLLFSVLIAGIRAWNAYYTNLGIESGFF
                     DNFYSALQTPIANANFPIEKQPRAQMSYCQMLWNKRNRAYPPAS"
     gene            complement(566..4117)
                     /locus_tag="PKM49_08675"
     CDS             complement(566..4117)
                     /locus_tag="PKM49_08675"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY82906.1"
                     /translation="MPNQSNERNKYVFISYSHHELPEVARRLKTDLEALGYEVFLDID
                     RIAAGGHWDATLNEGLQRLKDGGWFVFLMTPSSVRQSHNFCLNEVTWASYHTRDAILP
                     LRLVPCEPPLAIYTIQYYPIDDAIPIEEKEERYLEHLKEIEAFLSRKKTVPVQDERIR
                     LKQILQPMEFEPKRLAVLDRFMGREWVLDGVKKWLQAPNAPRIFRIGGGPGAGKSAIA
                     AWLVREMPDSIRAIHFCDRDNADKSNVNRIIHSIACQLAFCLEDYGRELKGKVFENEK
                     RYLNPTIQPQVLFEELIATPLVRVSRTQRNENQQIVILIDGLDEAGVPGRENELARLI
                     GDPELQKAMPTWIRWIITSRNEPLITFQLQGTPIQIDLDEEENQRADFRAYYEKRLSD
                     LHKEPMKEYLLETLVNRAERSFLYCELMCDDIVRNPGMLRSPEKFPVGMNGYLSRFFS
                     AHFPQGFDDRTIEILSLMCAAVEPLKADLILKITGMKKPELKKWLRAVGSLVQYEEKT
                     DTLRFYHASVLEWLTGELEGSQPDIYEIEPKEGHRMLADYGWEYLQGVQEWEEPLDYA
                     IENLGIHLYASNKEPEKDATVRDRLVELFKKSLPGEGKGRLPVKVIAALLTYVVARHK
                     ESEETGFKGIVDRFLKAETQPEAKHALAAFLHICYDYYSARGNAEWARWMHQRQHSVL
                     EELVDAYPENTGWKRDLSVSWNGIGDIEAKRGNLSQARDAYEKALAIMLELTEKDLIN
                     TAWLQDLGVSLYRIGDIEKAQGNLSQAQEAYEKYLAIMLKLSKIDPTNPEWLRDLSVS
                     LNQIGDIEAKRGNLDQASDAYEAALAIMLELIEKDLLNTVWFQDFGACLNRIGDIEKA
                     RGNFSEARDVYEKALAIMLKLTEKDPTNTDWLLGLSASWNRIGVIEAAMGNLAQAREA
                     YEKSLAIMLELTAKGQANADWMGDLSVSWSKIGDIETARGNLDQARGAYEKDLVIMLE
                     LTEKDPANTNWKLDLSVSWDKIGDIEAARGNLDQALVAYEKQQNIMLELAEIAPTNTD
                     WKRYLGVSWNNIADVEAKRGNIDRALEAYNKSLAIRLELAEKDPENLRWQLDYSISLS
                     GIGKLYQQKGEFPRSKECFLQVLDILRTLVAKAPDMVDSTIELAFALLDLYQVTENEY
                     EKQAYHVEAAQIAKSLVDTGVQHRDLNTLLNRLSIDR"
BASE COUNT         1005 a         1210 c         1065 g         1281 t
ORIGIN      
        1 gcttgctgga ggatacgctc gatttcgctt attccacaac atttgacaat aactcatttg
       61 agcgcgaggt tgtttttcta tggggaaatt ggcgttggca ataggagtct gcaatgcgga
      121 atagaagtta tcgaaaaacc cggattctat accgagattt gtgtaatacg cattccaggc
      181 ccgaatcccc gcaattaaaa cagaaaagag taataaaaga atgaatagcc atcgatttct
      241 taggatgaac gagaagattt tcatttttgg ccctcctaaa aagcgttttg atttgctagc
      301 cattattaac gcgacttcat ttaatcgagt tatcgtcata ggtttgagcg gagtggtaga
      361 gaaactcgat tctatcgaat tcacagagtc cgtccaatga ttcggggcgc tcaactctgt
      421 caggttaatg atcccagaca caataactca tgaaaaaggc gaccaaaaag gccaaaaaga
      481 acagctatgg cttttcgacc atcagaaatc cttagccaat tttgcattga aaaagcatac
      541 cgaaaacgcg cctagacgat ttcgtttatc gatcgatgga taagcggttc aataaagtat
      601 tcaggtcacg atgttggacc ccggtatcca caagggattt cgcgatctga gccgcctcga
      661 cgtggtacgc ctgtttttca tactcgttct cggttacttg atagaggtct aaaagggcaa
      721 acgccaattc aatagtcgag tctaccatat cgggcgcttt cgctaccagg gttcttaaaa
      781 tatccaacac ctgaagaaag cattctttcg aacgcggaaa ctctcccttt tgttgataga
      841 gttttccgat tccactcaaa gatatcgagt aatccagctg ccagcgtagg ttctcggggt
      901 ctttctccgc caattcaagc ctgatcgcca gcgatttgtt gtatgcttcc agcgcacggt
      961 ctatgttccc ccgcttcgct tcgacatctg cgatattgtt ccagctcacg ccgaggtacc
     1021 gcttccagtc ggtgtttgtt ggggctatct ccgccagttc aagcataata ttttgttgtt
     1081 tttcgtaagc gactagtgct tggtccaggt tcccgcgtgc cgcttcgata tccccgatct
     1141 tatcccagct tacacttaag tccagcttcc aattggtgtt cgcggggtct ttttcggtca
     1201 gttcgagcat gatcaccagg tctttctcgt aggcgccccg cgcctgatcc aggtttcccc
     1261 gcgccgtttc gatatccccg atcttgctcc agctcacgct taagtccccc atccaatcgg
     1321 cgttcgcctg gcctttcgca gtcaattcga gcataatcgc cagggatttc tcgtaggctt
     1381 ccctcgcctg tgcgaggttc cccatcgccg cttcgataac cccgatcctg ttccagctcg
     1441 cgcttaagcc aagcagccag tcggtgttcg tgggatcttt ctccgttagt ttgagcataa
     1501 tcgccagggc tttctcgtag acgtccctcg cctctgagaa gttcccccgc gccttttcta
     1561 tatccccgat cctgttcaag catgcgccga aatcttggaa ccagaccgtg ttcaggaggt
     1621 ctttctctat cagttcaagc attatcgcca gcgctgcctc gtaggcgtca gacgcttggt
     1681 cgaggttccc tcgtttcgct tcgatatccc cgatctggtt caagctcacg cttaggtcac
     1741 gtagccattc ggggttcgtt gggtctatct tcgacagttt gagcataatc gccaggtatt
     1801 tctcgtaggc ttcctgcgcc tgcgacaggt tcccctgcgc tttttcaata tccccgatcc
     1861 tgtacaaact tacgccgaga tcttgcaacc aggccgtgtt tataaggtct ttctccgtca
     1921 gttcaagcat aatcgccagg gctttctcgt aggcgtcccg cgcctgcgac aggtttcctc
     1981 gtttcgcttc gatatccccg atcccgttcc agctcacgct caagtcccgc ttccagccgg
     2041 tgttctccgg atacgcgtcc acaagctctt cgagaacgga atgctgtctt tggtgcatcc
     2101 atcgcgccca ttccgcgttc ccgcgtgcgc tgtagtaatc ataacaaata tgaaggaagg
     2161 cggctaaggc gtgttttgcc tccggttgcg tttctgcttt cagaaaacga tccacgatac
     2221 ctttgaatcc ggtttcttcc gattctttat ggcgcgcgac tacataggtc agaagggcgg
     2281 cgatgacttt taccggaagc cttcccttcc cctctcccgg gaggcttttc ttgaacaatt
     2341 caaccagccg gtcccggacg gtcgcgtcct tttccggttc tttgttagaa gcgtacaaat
     2401 gaatccccag gttttctatc gcgtaatcca ggggctcttc ccattcctgc accccttgaa
     2461 ggtactccca gccatagtcg gcaagcatcc ggtgaccttc tttaggttct atttcgtaga
     2521 tgtcaggctg cgatccttcc aattctcccg tgagccattc caggacggat gcgtggtaaa
     2581 aacgcagggt gtctgtcttt tcttcgtatt gaaccagaga gcctaccgcg cgtaaccatt
     2641 ttttcaattc cggtttcttc atcccagtga tcttcagaat caagtcggct ttcaaaggtt
     2701 cgaccgccgc gcacatcagc gataaaatct ctatggtacg gtcatcgaat ccctgaggaa
     2761 aatgtgcgct gaagaatcgg ctgaggtatc cgttcatccc tactggaaac ttttcgggtg
     2821 aacgaagcat cccggggttt ctcacgatgt catcgcacat aagctcgcag tacaaaaagc
     2881 ttctctctgc cctgttcacc agtgtctcca gaaggtattc cttcattggt tctttgtgta
     2941 aatcggaaag tcgtttctcg tagtaggctc tgaaatctgc cctttggttt tcctcttcat
     3001 ccagatcgat ctggatcggg gtgccttgaa gctggaacgt gatcaagggt tcattccgcg
     3061 aggtgatgat ccaccgtatc caggtgggca tcgctttttg gagttccggg tctccgatca
     3121 atcgagccag ttcgttttcc cttccaggaa ctcccgcttc gtcgagcccg tcgatgagga
     3181 tgacgatctg ttgattttcg tttctttgtg ttcttgatac acgaaccaaa ggggtcgcga
     3241 tcagttcttc gaaaagtact tgcggttgga tcgtagggtt caagtaccgt ttttcatttt
     3301 cgaagacctt gcctttgagt tcacgtccat agtcttccag acaaaaagcg agttggcatg
     3361 cgatcgaatg aatgatccga ttgacgttgg atttgtccgc gttgtcccgg tcacaaaagt
     3421 gaatcgcgcg gatggagtcg ggcatttccc ggaccaacca tgcggcgatc gcgctctttc
     3481 cagccccggg ccccccgccg atacggaaaa tcctcggggc gttcggcgct tgaagccact
     3541 ttttgactcc gtctaagacc cattctctgc ccataaatcg atcgagaaca gcgagtctct
     3601 tgggttcgaa ctccataggt tgtaggattt gtttgagtct gatgcgctcg tcctgaacgg
     3661 gaacggtttt cttccgcgag agaaaggcct cgatttcctt taaatgttcc aggtagcgct
     3721 cttccttttc ttcgatgggt atggcgtcgt cgatgggata gtactggatg gtataaatgg
     3781 ctaacggagg ctcgcagggt acgaggcgca aggggagtat cgcatcgcgg gtgtgataac
     3841 ttgcccaggt tacttcgttg aggcagaaat tatggctctg ccgaactgag gaaggagtca
     3901 taagaaatac gaaccatccc ccatctttca gacgctgtaa cccttcattc agcgtagcat
     3961 cccaatggcc cccggcggcg attcggtcga tgtctagaaa gacttcgtac ccaagagctt
     4021 ccagatccgt tttcaatctc cgggccactt ccggcagctc gtgatggctg tagctgatga
     4081 agacgtattt gtttctctca ttactttgat tcggcataaa tatcactccc gttttatttg
     4141 taaagcaaaa cggtagcagg tctttgagca cgtaccttgc ccgtttaagt cccgaaaatc
     4201 taacggtttg gtccgaatgc ctatcaactc cgcattccac attctttcct tatcacccgc
     4261 tcttcgttgt gtaataagat aggcccttca ccgatcctcc gcaccggaca acaaaatctc
     4321 atttcagttt ctatggaaat aatgacaatt atcgcatagg acatagcgtt tttcaatacg
     4381 atgtgtgagc ataatatcga gcgtgaccaa aattagaaag aagaagaagg gaaaaaacag
     4441 cctccaaagg agccatcatt tgagctcttt tgacccttca cgggggtgaa gaagtgtatt
     4501 tttcatccgc gtgaaaaacc tgccaacttg aaagttggcg tttccaagaa tcttctctcc
     4561 g
//
LOCUS       DAOPXN010000182         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19162_length_4542_cov_4.848897, whole genome shotgun sequence.
ACCESSION   DAOPXN010000182 DAOPXN010000000
VERSION     DAOPXN010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19162_length_4542_cov_4.848897"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PKM49_08680"
     CDS             <1..412
                     /locus_tag="PKM49_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY82907.1"
                     /translation="LAAVHDNIADFPEGYRTLVGERGITLSGGQKQRVAIARALMKKT
                     PVVILDDCLSAVDVETEMHILRSLEDEIADRTTILVSHRLKAVASADLIVVFDNGQIA
                     ESGTHVELLEKNGMYATLYKKQLLEEEIKRRPTP"
     gene            409..2373
                     /locus_tag="PKM49_08685"
     CDS             409..2373
                     /locus_tag="PKM49_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82908.1"
                     /translation="MSASKDYHLEKEEEGKGFDYHLFKRLWAYVTPYRKLFYLSLLML
                     VLGTLFQLYIPYVSKNAIDAFMMREHRYTSSAVEAKGYYRLDEGLWIKEDKGGAFSLT
                     RRDGHFVLTDGLLSFRLSQEQIRELRVDDIQGIDKSVLILLVLLFSVFFTNYIQVYFS
                     NKLGQKVIYDIRTGLFRKVLTLPLKFYDHNPTGRIVTRITNDTENLNEFFTSAVTSIV
                     KDILLLIGIIGFMLFLSSQLTGYSLFVLPVIVLATALFRFFDRKAYRKVRVRLARINA
                     FLAEHLSGMSVIQLFNRETSKYEEFKKTNHSYYRATLQQLTVFAVFRPTMDILFYIAL
                     CVVIWFGSKDFVSGIMTFGVLYAFINYIDMFFQPLRDLSEKFDIVQNALASAEKIFKL
                     LDEKASEIEPSQEGPEIRDGSVEFESVTFGYNEGVEVLKDISFNIRPGEKVAIVGETG
                     AGKTSLIEILTALYPIQKGRILIDGIPIDAYQVHALRKQVAVVLQDVFIFSGDVVDNI
                     RLFDNAVSREEVIDAAKYVNVDHLISRFEKGYDTMLNERASTLSAGERQLIALARAVV
                     RDPKILVLDEATANIDPVTEAFIQDAMEKVTQGRTVITIAHRLSTIRNADRIFVFHKG
                     RLVESGTHLELFAKNGLYTQLYRLQYQLNE"
     gene            2424..3137
                     /locus_tag="PKM49_08690"
     CDS             2424..3137
                     /locus_tag="PKM49_08690"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY82909.1"
                     /translation="MHSNFSFDSRMTIPQIVERAEALGIDRFIITDHYENVSDSGLRR
                     FFIPDPEEHLAQLRAYGLPTGLEFGWDTETPIRYPVEAYDFVLLSFHNLKRSGFRGAI
                     DYDAYLEDVWNGVNLFRHFTVLAHLDLPRRYVEGNPPFSQQHYPHFERIFKKLIEDGK
                     GIELNCSTIRQYGQPNPARDLLELYFNCGGKVITLGSDAHKIDDIGKDLLKGRAILEE
                     IGFRYLTVFEDGEYTFRPL"
     gene            3156..4529
                     /gene="murD"
                     /locus_tag="PKM49_08695"
     CDS             3156..4529
                     /gene="murD"
                     /locus_tag="PKM49_08695"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNY82910.1"
                     /translation="MYDICFLGYGVSNQGALRLFLKEYGGKAFVSTLSRLTPSERQRF
                     EAESVDWEEGQNSDRCLLCQRIVVSPGIPPKTPILTAAYEKGIPVCSDIDFISPLYPP
                     GILTVGITGTNGKTTTVELTAHLLSSVMRSTTVGNIGFSPADLILEERLSETDAVVFE
                     LSSFQLYYTHIPFLNIAALLNITTDHLSWHTTFEAYVEAKRKVFALKKSPDRNRRNLS
                     SLRLTAGAPVDFSVGYEPNDSCQITEHPRALKIHTDQGPISLSLEGYTLKGGHNDRNA
                     AFAASIAYLSGVSLERIQNGLSTYKGAEHRLEEFLVHRGVTYVNDSKSTNADSLIQAI
                     KTYRGEGGRLLLLMGGRGKGEDYTSLASEIRKGCHHVFLCGENAPLIAPLIENHLPVS
                     RYSNWTEAIAQAIAQAREGDTVLFSPGGSSFDFFNNYIERGLYFKKACRENLSRLSTG
                     FGEKGKK"
BASE COUNT         1139 a         1184 c         1171 g         1048 t
ORIGIN      
        1 actggcggcc gttcacgaca acatcgccga tttcccggag ggttatcgta ccctggtggg
       61 cgaacgggga atcacgcttt cgggaggtca gaaacagcgg gtagcgatcg cccgagccct
      121 tatgaaaaaa actccggtgg tcatattgga cgactgcctt tccgccgtcg acgtcgaaac
      181 ggagatgcac atcctcagga gtttggaaga cgaaatcgcg gacagaacca caatcctggt
      241 ctctcaccgg ttgaaggcag tcgcttcggc cgacctcatc gtcgtcttcg ataacggtca
      301 aatcgccgaa tcgggcacgc acgtcgagct cttggagaaa aacgggatgt acgcgacctt
      361 gtacaaaaaa caactcctcg aagaagagat caagaggagg cccacgccgt gagcgcgtct
      421 aaagactacc acctggagaa ggaagaagag gggaaaggtt tcgattatca ccttttcaaa
      481 cgcttgtggg cgtacgtgac cccctatcgc aaactgtttt acctatcttt gctgatgctg
      541 gtcctcggca cgcttttcca gctgtacatc ccttacgttt cgaagaacgc gatcgatgcc
      601 tttatgatgc gcgaacaccg gtacacctct tccgcggtag aagcgaaagg gtattaccgc
      661 ttggacgaag ggttgtggat aaaagaggat aaaggaggag cgttttcttt aacgcggaga
      721 gacggccact tcgttctcac cgatggactc ctctctttcc gcttgagcca agagcaaatt
      781 cgggaactgc gggtggacga tatacaaggg atcgacaagt cggtcctcat cctgttggtg
      841 ttgctgttct ctgtgttctt caccaactat atccaagttt atttctccaa caaactgggg
      901 caaaaggtca tctacgatat ccgcacaggg ttatttcgga aagtcttgac cctcccgttg
      961 aagttttacg accataaccc gacgggcagg atcgtgaccc gcatcacaaa cgataccgaa
     1021 aacctcaacg agttcttcac atccgctgtg accagtatag tgaaggacat cctcttgttg
     1081 atcggaatca taggctttat gctcttctta agcagccaat tgaccggtta ctccttgttc
     1141 gttctaccgg ttattgtatt ggcgacggcc ttattccgtt tcttcgatcg gaaagcgtat
     1201 cgaaaagtcc gcgtgcgctt agcccgcatc aacgcgtttc ttgcggaaca cctgtccggg
     1261 atgtccgtaa tccagctgtt taatcgagaa acgtccaaat acgaagagtt caagaaaacc
     1321 aatcactcct actatcgcgc aaccttgcag cagctcacgg tgttcgccgt cttccgaccc
     1381 acgatggata tcctgtttta tatcgctttg tgcgtggtga tatggttcgg ttccaaagac
     1441 ttcgtctccg gcattatgac ctttggtgtt ctttacgcat tcatcaacta catcgatatg
     1501 ttcttccaac cgcttcggga cctttccgaa aaattcgaca tcgtacaaaa cgctctcgcc
     1561 tcagccgaaa agatcttcaa attgctcgac gaaaaggcct cggaaatcga gccttcccaa
     1621 gaaggccccg agatccgcga tggttccgtt gaattcgaga gtgtgacctt cggctacaac
     1681 gaaggcgtgg aggtgttgaa agacatctcc ttcaacatcc gccctggaga gaaggtcgcg
     1741 atagtggggg aaacgggcgc agggaaaact tctcttatcg agatactcac cgcgctctac
     1801 ccgatacaaa aagggcggat tttaatcgac gggattccga tagacgccta tcaagtccac
     1861 gccttacgca aacaggttgc ggtcgtactg caggatgtct tcattttttc gggtgacgtc
     1921 gtggacaata tccggctctt cgacaacgcg gtctctcgag aagaggtgat cgacgcggcc
     1981 aaatatgtta atgtcgacca tttgatctcg cggttcgaaa agggttacga tacgatgctc
     2041 aacgaacggg cctctacgct ttcggccggt gaaaggcaac tgatcgccct ggcgagggcg
     2101 gtcgtgaggg atcccaagat cctggtattg gatgaagcga cggccaatat cgaccccgtc
     2161 accgaggcgt tcatacagga cgcgatggaa aaagtgactc agggacgcac cgtcattacc
     2221 atcgcgcacc ggctctcgac gatccgaaac gccgatcgga tttttgtttt tcacaaagga
     2281 cgactcgtcg aaagcggcac gcacctcgaa ttgttcgcca aaaacgggct ttacacacaa
     2341 ctctatcgtt tgcaatatca gctgaacgaa tgaattaagg gggtccgatc tctatttcca
     2401 caccattttt aatcgactat catatccact ccaacttttc ttttgattcc cgaatgacca
     2461 taccccagat cgtggaaagg gccgaagcgt tgggcatcga tcgattcatt atcacagatc
     2521 actacgaaaa tgtgagcgac agcgggttac ggaggttttt catcccagac ccggaagaac
     2581 atctggcgca actgagagcc tatgggcttc ctacgggtct cgaattcggc tgggacacgg
     2641 aaacgccgat tcgctacccg gtcgaagctt acgacttcgt cttgctcagc tttcacaatt
     2701 taaaaaggag cgggtttaga ggagccatcg actacgacgc ctatttggag gatgtgtgga
     2761 atggggtgaa cctgtttcgg catttcaccg tcctcgcgca tctggacttg ccgaggaggt
     2821 atgtagaagg taatccccct tttagtcaac agcattatcc ccacttcgag cgcattttta
     2881 agaaattgat cgaagacgga aaggggatcg agttgaactg ctcgacgatt cgtcagtatg
     2941 gacagccgaa tcccgctaga gatctccttg aactctattt caactgcggt ggaaaagtga
     3001 tcaccttagg ctctgacgcc cataaaatcg atgatattgg aaaagacctc cttaaagggc
     3061 gggcgatact cgaagagatt gggtttcgat acctcaccgt cttcgaagac ggagagtata
     3121 ccttcagacc tctctgaggg gaaaggaagg gcacggtgta cgacatctgt tttttagggt
     3181 acggggtgag taatcaaggt gcgctgagac tatttttgaa agagtacggc ggaaaggctt
     3241 tcgtgtcaac ccttagccgc ttgaccccat cagaaaggca acgcttcgaa gccgaaagcg
     3301 tggactggga agaaggccag aattctgaca gatgcctgct ttgccaaaga atcgtcgttt
     3361 cgcccggtat cccaccgaag acaccgatcc taacggccgc ctacgagaag ggcatccccg
     3421 tttgtagcga tatcgacttc atctcgcccc tctatcctcc ggggatcctg acggtgggaa
     3481 tcacaggaac caacggtaag accacaacgg tggagttaac ggcccacctt ctatctagcg
     3541 tgatgcgttc gacaacggtc gggaacatcg ggttctcccc cgctgatctg atcctggaag
     3601 agcgtctctc cgagaccgac gccgtcgtat ttgaactcag cagctttcag ctgtactaca
     3661 cgcatatacc atttctgaac atcgcggcgc tactaaatat cacgaccgat catctcagct
     3721 ggcacacgac cttcgaggcg tatgtggagg cgaaacggaa ggtattcgcc cttaaaaaga
     3781 gtccggaccg taaccgacgc aacttgagca gcctccggtt aaccgctggg gcgcccgtgg
     3841 atttttcggt cgggtacgag cccaacgact cgtgtcagat tactgaacat ccgcgcgctt
     3901 tgaaaatcca tacggatcaa ggacctatct ccctctcttt ggagggctat acgctcaaag
     3961 gagggcataa cgatcgaaac gcggccttcg ccgcttctat cgcttacttg agcggcgtgt
     4021 cgctagaaag gatacagaac ggtttgtcca cgtacaaagg ggcggagcat cggttggaag
     4081 agttcctggt ccaccgcggt gtgacctacg tcaacgattc caaatccaca aacgcggact
     4141 cattaataca agcgattaaa acctatcggg gagagggtgg gcgactgctt ctacttatgg
     4201 gagggagagg aaagggcgag gattacacgt ctctggcctc cgaaatcagg aagggctgcc
     4261 accacgtgtt cctatgtggc gaaaacgcgc cgcttatagc ccctctaata gaaaaccacc
     4321 taccggtctc ccgctattcg aattggacgg aggcgatcgc gcaggccatc gcgcaggcga
     4381 gagagggaga caccgttctt ttcagcccag ggggttcgag tttcgacttc ttcaacaact
     4441 atatagagcg agggctgtat tttaaaaagg cttgtcgcga aaacctgtcc cgactttcga
     4501 ccggttttgg agaaaaaggg aaaaaataag aaaaaacaac gc
//
LOCUS       DAOPXN010000183         4504 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19367_length_4504_cov_5.218027, whole genome shotgun sequence.
ACCESSION   DAOPXN010000183 DAOPXN010000000
VERSION     DAOPXN010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19367_length_4504_cov_5.218027"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(437..1648)
                     /locus_tag="PKM49_08700"
     CDS             complement(437..1648)
                     /locus_tag="PKM49_08700"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82911.1"
                     /translation="MKNYRYFWLGQMVSLIGTWMQTSAQAWLVLEITDSPLLLGLLGV
                     AQFTPVLLFSLLAGVFVDRFSKRTLLLYTQAISMGLAFVLALLVLSGRVAYWQVFLVS
                     LSLGIIKTVDIPARQSFMTELVGKKNLLNAIALNSTIFNLARIVGPVLSGMMMGWVGA
                     GWCFFINGVTFLAVLIGLWKIELPNVGFRKTRERVLPELLAGLRYVLRHRTLFETIAL
                     LAIASIFTMNYNVWIPVLARNQLGLGASGYGFLMASLGVGAVIGSLLLASNSRKGIRQ
                     GLLRALPILTALLLIMTGLTKQYGLAVVLVAGIGMLNTAFTTTANSSMQVLSDDEYRG
                     RVMSLYALVFTGSSPIGNTFVGWLSDAYTVSPALILSGSLALILLVFTGLVSRNGKKT
                     RSRDSAFQGDS"
     gene            complement(1882..3063)
                     /locus_tag="PKM49_08705"
     CDS             complement(1882..3063)
                     /locus_tag="PKM49_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82912.1"
                     /translation="MSQAQLPLETAILLIAGLMATLTGIFLFPVGTGALPYYENGLYG
                     LFLFIFALQMLTLGKTPFGDVSRSIPVIVIGIGIAITGIVTCFIPDLLSHIPRLLLFI
                     CFAPGGALLLLQMIFSKDKLRNWLRLGSLFRHLIFSCAAVYLLSILIGWLVLMSPKID
                     VAITALFVLTFGIAILYLAVVLRKVYLTYPEAETKDPGTGILSTDKMLLLVIGLFMVV
                     LGALLIPVSLGQLPFAGSAQLGLLMVIFSIQMLALGSTPIGAFPRSRAMILVGFVFAG
                     LGIISAVIPGILVGPLTLLIGLLNLFGGAISLGKMVFSKNQTPAGSPQTVLSVLRRLF
                     ITQLALALLSIMFGTSMLIPSLLPGLLVGVILFANGCLLLYLIYILISLDKVIKQLSQ
                     A"
     gene            complement(3097..3900)
                     /locus_tag="PKM49_08710"
     CDS             complement(3097..3900)
                     /locus_tag="PKM49_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNY82913.1"
                     /translation="MSQWLNNVAVVTGAASGIGLGLTEHLLKLGAKAVFMGDLKEENL
                     TKESGRLKQQYPGKVFPILTDVTKREQVESLIREAKAFDGHLDIVFNNAGMGMTVPTE
                     QVTFDIWKFIMDLNFMGVVYGTYTAIPIMREQGFGRIVNTGSIVGRVPAPYQAVYAAS
                     KSAVISMTESLQYELEVEGLSFGVICPGNVRTAIFGSLAPPPGSISVEDAMEIIFQEL
                     EKKTLIIILPDEVREYDRMYREDRAGFDQFARNLAAERRENYRTKGTYY"
     gene            complement(3914..>4504)
                     /locus_tag="PKM49_08715"
     CDS             complement(3914..>4504)
                     /locus_tag="PKM49_08715"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HNY82914.1"
                     /translation="EWMSGGDYRLIQVTIPTTFKGAGDTQNLDGDYALVVWENNTWPI
                     LGGREEDGVPKIFADIARERHVGEHWFTAASYESRTFLTIDFERKAQASQDTLEKLKE
                     QSKINLFGWRYLANLGKGGAALSQATLYPQEVLIRQLWTGEGSVQWTKLLPHWHPGQY
                     SIIQAFAELPIIRYNEAKMMVCSASLNVGDSRVLTE"
BASE COUNT         1161 a         1228 c         1145 g          970 t
ORIGIN      
        1 cgcacaggat gagtaagata tgccaactta aaagttggcg ttcccagacg ttcccaagcg
       61 cagcatccag gcgtaagcgc cccttcttcc cgcgagggcg cgatacgctt ctatgaaaga
      121 caaaaagcgg atcgcggggt cgtattataa gccacttacc ccgcgatgaa ccacaaagtc
      181 cggcgttccc aggaaacctg ccaactaaaa gctggcgttc ccagggcgtt tctctatgtt
      241 ctctctgtgc tctctgtgta ctctgtgcga aaccatcttt tgattttgaa aagacatgcc
      301 aacttaaaag taggcgttcc caaagcgttc ccaagcgttt ccagcagcgc gaccccaaaa
      361 gcccaaaagg ctctccgatt gcaaagttgg cgccaccagg tgctccggtg aggagctatt
      421 attcctctct atttgcctat gaatctccct gaaacgctga gtcgcgagaa cgcgttttct
      481 ttccgttgcg agaaacaagt ccggtgaaaa ctaggaggat cagggccaag ctgcccgaaa
      541 gaatcaaagc cggtgagacg gtgtaagcgt cggatagcca gccgacgaag gtatttccta
      601 tcggcgaaga ccccgtgaaa accagcgcgt agaggctcat cactcgtcct cgatattcat
      661 cgtccgacaa cacctgcatg gatgaattcg ccgtcgtggt gaaagcggtg ttgagcatcc
      721 cgattccggc gaccaagacg acggcgagcc catactgttt cgtgagtccg gtcataatga
      781 gcagcagcgc cgtcaggatt ggcagagcgc gtaacaatcc ctggcggatt ccttttcgac
      841 tgttcgaggc taagagcaag gagccgatga cggcgcccac gcctaaactc gccatcagaa
      901 aaccgtatcc ggatgcgccc aagcccaatt gattcctggc cagcaccgga atccacacgt
      961 tgtagttcat cgtaaaaatg ctggcgatag cgagaagcgc gatcgtttca aagagtgtac
     1021 ggtgacgcag gacgtaccga agtccagcta ataactcggg tagcacgcgc tcgcgcgttt
     1081 tcctaaatcc cacattaggg agctcgatct tccacaaccc gatcaacacc gcgagaaagg
     1141 tgacaccgtt gataaaaaag caccagcccg cgcccaccca tcccatcatc atacccgaaa
     1201 gcaccggccc aacgattcgg gccaaattga agatcgtgga gttgagggct atcgcgttca
     1261 gaaggttctt tttgccgacc agctccgtca taaacgattg tcgggcagga atatccacgg
     1321 tttttattat ccctaaggac aatgaaacga gaaaaacttg ccaataggcc acgcgaccgg
     1381 acagcaccaa aagagccaac acaaaggcca accccatgga aatggcctga gtgtataaaa
     1441 gaagcgttcg cttcgagaag cgatccacga aaactccagc cagaagggag aataaaagaa
     1501 cgggggtaaa ctgcgcgaca cccaacaacc ccaataacag gggcgagtcg gtgatttcca
     1561 aaaccaacca agcttgggca gaggtttgca tccaggtccc gattaaggaa accatctgcc
     1621 caagccaaaa gtatcggtag ttttttatac ctaaagccgg gaaggcctcg cgtagtttag
     1681 ccttcagcga tgcgcgagac agatcggtcc cttcctttct cggagagacg cgccgtttgc
     1741 gtttaggtcc gggcgcttct ttcctccaat ttctatatca taccgatcta cgaagggaga
     1801 tcttctactt cttctacaac aaacaaaagc ttcgtaccct tcccgactat tgtaccaaaa
     1861 tccacgaggc ctttgggatc ctcacgcttg actcagctgt ttgataacct tgtccaggga
     1921 tataagtatg tagatcaagt acaaaagaag gcagccgttc gcgaacaaga tcactccgac
     1981 aagaagtccc ggcaggagcg agggtatcaa catcgaggta ccgaacataa tagagagaag
     2041 ggccaaggca agctgagtga tgaatagcct tctcaagaca gaaagaaccg tctgggggga
     2101 gcccgccggc gtttgatttt tggaaaaaac cattttgccg agggagatgg cccctccgaa
     2161 gaggttcagc aacccgatga ggagggtcaa aggtcctacc agaattccgg gaatcaccgc
     2221 agatatgata cccagccccg cgaacacgaa tccgaccaag atcatcgccc gactgcgcgg
     2281 aaatgctccg atcggggtgc tgcccaaggc cagcatttgt atcgaaaaga tgaccatcag
     2341 caggccgagc tgcgcgctcc ccgcgaacgg gagttgaccc aaagacacgg gaatcagcaa
     2401 agcgcccagc accaccatga agagtccgat gacgaggagg agcattttgt cggttgaaag
     2461 gatgccggtt ccggggtctt ttgtctccgc ctccggatag gtcaggtata cctttcgaag
     2521 gacgaccgcc aggtagagga tcgcgatgcc gaaggtcagc acaaacagag ccgtaatcgc
     2581 gacatcgatc ttgggcgaca tcaagacgag ccatccgatg agaatggaca gcaggtagac
     2641 ggcggcgcaa ctaaaaatca ggtgtcgaaa gaggcttccc aatcggagcc aattccggag
     2701 tttatccttg gaaaagatca tctggagcaa cagcagcgcc ccgcctggag caaaacagat
     2761 gaacagcaag aggcggggga tgtggctgag caggtccgga atgaagcagg tcacgatgcc
     2821 cgtaatcgcg atgccgattc cgatgacgat aactggtatc gaccggctca cgtcaccaaa
     2881 aggggtttta ccgagcgtga gcatttgcag ggcgaagatg aacaggaata acccatacag
     2941 gccattttcg tagtaaggca acgcgccagt acctaccgga aagagaaaga taccggtcag
     3001 tgtagccata agcccggcaa tcagtagaat cgccgtttct agcggaagct gagcttgcga
     3061 caagaagaaa tcgcgtattc gttctctcat acgaaattaa taataagtcc ctttggtacg
     3121 gtagttttca cgccgttctg cagccaggtt gcgggcgaat tgatcgaacc cggcacggtc
     3181 ttctcggtac atgcgatcgt attcacggac ctcgtcgggc aggatgatga tcaatgtctt
     3241 cttttccaat tcctggaaaa taatttccat tgcatcctct acggagatcg agccgggagg
     3301 tggggcgagc gatccaaaaa tggcggttcg gacgtttccg ggacagataa cgccaaagct
     3361 cagcccttcg acttcaagct catattgcag gctctccgtc atagagatga ccgcgctttt
     3421 actcgcggcg tacaccgctt ggtagggcgc ggggaccctt ccgacgatag acccagtgtt
     3481 cacgatgcgg ccgaagcctt gctcgcgcat aataggaatc gcagtgtacg tgccgtacac
     3541 cactcccata aaattcaaat ccataatgaa tttccagata tcaaaggtga cttgttccgt
     3601 aggcacggtc atccccatac ccgcgttgtt gaacacgatg tccaaatgcc cgtcaaaggc
     3661 tttggcttca cgtatcaggc tctcaacctg ttcccgttta gtgacatcgg tgaggatcgg
     3721 gaaaaccttt ccggggtatt gttgtttcaa tctccccgac tccttggtca agttttcctc
     3781 tttcaagtca cccataaaaa cggctttggc cccaagcttc aacagatgct ctgtgagacc
     3841 caacccgatt ccggaggccg cgccggttac gacggctacg ttatttaacc attggctcat
     3901 cagacatcac tccttattcc gttaatactc tagaatcgcc gacgttgagg ctggccgaac
     3961 acaccatcat tttcgcttca ttgtatctaa ttatcgggag ttcggcaaac gcttgaatga
     4021 tactgtattg cccaggatgc caatgcggta agagcttggt ccattgtacg cttccttccc
     4081 cggtccaaag ctggcgaatt agtacttctt ggggatacag ggtcgcttgg ctcaacgccg
     4141 cccctccctt gcctaagttg gccaagtatc gccaaccaaa cagattgatc ttcgattgtt
     4201 ctttcaattt ctcgagcgtg tcctgcgacg cttgcgcctt tctttcgaag tcgatggtga
     4261 gaaaggtacg gctttcgtaa ctcgccgccg tgaaccagtg ctcgccgacg tggcgttcgc
     4321 gggcgatatc ggcgaaaatt ttgggtaccc catcttcttc tctaccgccc aggatcggcc
     4381 aggtattgtt ttcccaaacg accaacgcat agtcgccatc aaggttttgt gtatctcctg
     4441 cgcccttgaa cgttgtcggg atagtcacct gaatcaatcg gtaatcgcct cccgacatcc
     4501 attc
//
LOCUS       DAOPXN010000184         4460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19639_length_4460_cov_4.446992, whole genome shotgun sequence.
ACCESSION   DAOPXN010000184 DAOPXN010000000
VERSION     DAOPXN010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19639_length_4460_cov_4.446992"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..3630)
                     /gene="smc"
                     /locus_tag="PKM49_08720"
     CDS             complement(67..3630)
                     /gene="smc"
                     /locus_tag="PKM49_08720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNY82915.1"
                     /translation="MQLDQIRLAGFKSFAKPVTLSLSEGITAVVGPNGSGKSNIVDAI
                     LWVFGEPSLKSIRATEKTDIIFAGSDHQVAAPYADVTLCFRNADKSFEVSRHLTRDGK
                     NDYFINEEKARRKDILDLFEGTGAGKELYSIVSQGRVDKILNSSSEEIRLLIEEAAGI
                     AVYKRKKKETLSKILSVEDNLNRIGDILAELDKQRKSLYLKAKRAEKFQEYTQTLKAV
                     QKEYFSHLTHKLKDQKRASEAETCRLQEALKDLQKEIARTESQWYELKEEFSSSNQEM
                     EGFTSVLEQYKERQNHLQDLRTLYLTQQSEKESQYVESSTRLDTIDKENVKMTNRLEE
                     LRLLFDSVNANIESISEELSGLEGQKESLSNQYTEEEKEILELQQRYAQQDKTLGKLE
                     NELLRLEETIEDHTKRLVVIKNQIESKTERLEGHQNELDELARSISESSQKETQLVND
                     LNRLKQDLVAVEERIRAVETEKYELIQRVKHIDLEKNLLEKQIREFSGYTRPVKALFQ
                     RKETDIGFRNMIDVVANLLEVNREYEKAFEVLLMGKAQNIVTLDTPTAKYGVEVLKSN
                     GWGRATFLPLDTLEFKPVVQNPKLLKEAGVIGYAAELVAIDKDFSKLPLYLFKDTLLV
                     ETLDDGLRLKKLYQLKNQIVSLDGQFIASSGAITGGSLDLEPSTSLLVRNRKVHELQE
                     ELDRANRSLQKIETEEKAQLKQKHELSGLRAVMESELNDLLLKNSSIRRTVEEIHHAV
                     AELSKEIGELQKLRIDYEAKIAGASARKEKAIEEIGSGKVSLTALQSALDKYGQVLRE
                     KKQTMQQLQERMNEVKLELNSRKERQRQYRAEIGDIESRTSENTEKRDEYKHRLLSLD
                     GEIQTLKEKTRQMDVELDSVNKEMAGLFNSMKYQQEERGQKMKKMEELEKSLASLKEE
                     RDTARDKIHKQELVAQEVELGYRHLYEKMSQAGLAPEEEAQMDEPVERIRFSEEEEES
                     KAAQLADLEQKIKYLGAVDLQAIEEHRDVEARHADLDGQRKDLVDAKASLYEILAKTD
                     KEAKDIFLKTFDAISRNFDEMIRTLFGGGNGELRLLPGADILETGVEISVKRPGKKHQ
                     KLYLLSGGEKSLVGIALVFSMLRINPSPFYILDEVDAALDDYNVERLKTLIEKNKDMA
                     QFIVITHNKLMMEGADILYGITQSMGISMVMAVELEKYAVS"
     gene            complement(3762..4358)
                     /locus_tag="PKM49_08725"
     CDS             complement(3762..4358)
                     /locus_tag="PKM49_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HNY82916.1"
                     /translation="MIDPASTRFIAIDTETTGLDPRKGARLTEIAAVTVLADRILEDE
                     VFQELMNPGVSIPLSISKLTGITNEMVKGKPGIKEVLERFFAWVPREAILIFHNAPFD
                     LSFLNHFSEKYGLEGLGNAYCDTVELWVSSQGGRCRLDEILNHLGIVLPHEMRHRAIG
                     DALGTALAFIKLAESIGPAALKRYIHSSGAWNRRPRTW"
BASE COUNT          870 a         1209 c         1082 g         1299 t
ORIGIN      
        1 gttcccccgt ccggctaggg tccacccggt actccaggat ttcagaactc tcttcgtcga
       61 ttcggttcat gaaacggcgt acttttccaa ttccacggcc ataaccatcg aaatgcccat
      121 cgactgcgtg atgccgtaga gaatatcggc gccttccatc atcagtttgt tgtgggtgat
      181 cacgatgaac tgcgccatat ccttgttctt ttcgatgagg gtttttaacc gctcgacatt
      241 ataatcgtcg agggcagcat ccacttcatc caggatataa aaagggctgg gattgatacg
      301 cagcatcgag aagaccaaag cgatcccgac gagcgacttt tctcctcctg agagtaaata
      361 gagtttctgg tgcttctttc cgggacgctt cacggagatc tcgacaccgg tttccaggat
      421 atcggcgccc ggcaacaacc tcaattctcc gtttccgcct ccaaacaacg ttcggatcat
      481 ctcgtcaaag ttgcggctga tggcgtcgaa ggtcttcaag aagatgtctt tggcttcctt
      541 gtccgtttta gccaggattt cgtagagcga ggctttcgcg tcaaccaggt cctttcgttg
      601 cccgtccaag tcggcgtgcc gtgcctcgac gtctcggtgc tcctcgatcg cctgcaggtc
      661 gacggcgccc aggtacttga tcttttgttc caagtccgcc aactgcgcgg ctttgctttc
      721 ctcttcctct tcggagaacc gaatccgctc taccggctca tccatctggg cttcctcttc
      781 gggagcgagg ccggcctggc tcatcttttc gtagagatgg cgatacccaa gctccacctc
      841 ttgggcgacg agttcctgct tatggatttt gtctctcgcg gtgtcccgtt cctctttgag
      901 cgaagcgagg gacttttcga gctcctccat cttcttcatc ttctgcccgc gttcttcttg
      961 ctggtacttc atgctgttaa acagcccggc catctctttg ttcacgctgt ccaactcaac
     1021 gtccatctgc cgggttttct ctttcagagt ttgtatctcc ccatcgagtg agagcaatcg
     1081 atgtttgtat tcgtctcgct tttcggtatt ttcgctcgtt cgcgattcga tatcgccgat
     1141 ctcggcgcgg tactgccttt gtcgttcttt tcgcgaattg agctccagct tcacttcgtt
     1201 catacgttcc tgcagttgtt gcatcgtttg cttcttttct cgcaaaacct ggccgtattt
     1261 atcgagcgcg ctctgtaagg cggttaagga gacctttccg gatccgatct cctcgatcgc
     1321 tttctccttc cgcgcgctgg cgcccgcgat cttggcttca taatcgatcc taagtttttg
     1381 taattcaccg atctctttcg atagctcggc cacggcgtga tgaatctctt ctacggtacg
     1441 ccggatggaa gaatttttca ataggaggtc gttgagttcc gactccataa cggctcggag
     1501 cccgctcaat tcgtgttttt gtttcagttg ggccttctct tccgtttcta tcttctggag
     1561 agaccggttc gctcgatcca gctcctcctg taattcgtgc accttacgat tcctgaccag
     1621 gagcgacgtg gaaggctcca ggtctaagct gccaccggta atcgcgcccg aagaggcgat
     1681 aaattggccg tccaaagaga cgatctggtt cttcaactgg tatagcttct ttagccttaa
     1741 cccgtcgtca agggtttcca cgagcagggt gtccttgaat aggtacaacg ggagcttgga
     1801 gaaatctttg tctatggcga cgagctccgc ggcgtagccg atcacgccgg cttctttcag
     1861 gagttttggg ttttgtacca ccggcttgaa ctccagcgtg tcgagcggca ggaaggtcgc
     1921 gcggccccaa ccgttgcttt tcaagacttc caccccatac ttggcggtgg gcgtgtcgag
     1981 cgtgacgatg ttctgcgcct ttcccatcaa gagcacttca aaggcttttt catactctcg
     2041 gttcacttcc aagagattgg cgacgacgtc gatcatattt cgaaaaccga tatccgtttc
     2101 ttttctctga aacaaggctt tcaccggccg tgtgtatccg gaaaactccc ttatctgttt
     2161 ctctaacaag tttttttcca aatctatgtg cttgaccctt tggatcagct cgtatttttc
     2221 tgtttcgacc gcgcgaatcc tctcttctac ggcaaccaaa tcctgtttca atcgattcag
     2281 atcgttaacc agctgcgttt ccttttgaga actctctgag atggatcgtg cgagctcgtc
     2341 gagttcgttt tggtgccctt ccaggcgttc ggtttttgat tctatctggt tcttgatcac
     2401 gaccagccgc ttcgtatggt cttcgatggt ttcttctaga cgcaacaact cgttttccag
     2461 tttaccgagg gttttgtctt gttgcgcgta ccgttgctga agctctaaaa tctccttttc
     2521 ctcttcggtg tattggttcg acaatgattc tttctgacct tccaatccac tcaattcttc
     2581 ggagatcgac tcgatgttgg cgttgacgga gtcgaacagc agccgtagtt cttcgaggcg
     2641 attggtcatc ttcacgttct ctttatcgat ggtgtcgagc cgcgtggaag attcgacgta
     2701 ctggctctct ttttccgatt gttgagtcag gtagagagta cgtaggtcct ggaggtggtt
     2761 ttgacgctct ttgtattgtt caagaacgga ggtgaagccc tccatctcct gattcgagga
     2821 agagaactct tctttgagtt cgtaccattg cgactccgtc cgcgcaatct ctttctgaag
     2881 gtccttcaac gcttcttgta agcgacaggt ttccgcttcg ctcgcccgct tctgatcctt
     2941 cagtttgtgg gtgaggtgag aaaagtactc cttctgtacg gccttgaggg tctgcgtgta
     3001 ttcttggaat ttttcggcgc gtttcgcttt caaatacaaa gacttccttt gtttgtcgag
     3061 ttctgccagg atgtcgccga tccgattgag gttatcctcg acgctcagga ttttcgacag
     3121 cgtctctttt ttctttctct tgtaaacggc tatccctgcc gcctcttcga tgaggagtcg
     3181 aatctcttct gacgagctgt tcagaatctt gtctaccctt ccctgactga cgatggagta
     3241 aagctctttt ccggcgcccg ttccttcgaa aaggtccagg atatccttcc gtcgcgcttt
     3301 ttcctcgtta atgaaataat cgtttttccc gtcgcgggtt aggtgccgcg atacctcgaa
     3361 ggatttgtct gcgttgcgaa aacagagggt gacatccgcg taaggagcgg ccacttggtg
     3421 gtcgcttccg gcgaagatga tatcggtttt ttccgtcgcg cggatgcttt tgagagaagg
     3481 ttccccgaag acccacagga tggcgtcgac gatgttcgac ttccctgaac cgttcggtcc
     3541 gacgaccgcg gttatccctt ctgaaagcga caaagtcaca ggtttcgcga aagacttaaa
     3601 accggcaagt cgtatttggt ccagttgcat ttcgttttcc cccccacaca aacgagacaa
     3661 ccacctcaaa aacataaaag aatatttggt taaataatag catattgcga ttctgaaagc
     3721 aagtatttgt cataaacgat caaaccgatg cttagcagtg tttaccacgt acgtggtcgc
     3781 ctgttccaag cgccgctgga atgtatgtat ctcttcaaag ccgcggggcc gatggattcg
     3841 gcgagtttta taaaagcgag cgcggtgcca agcgcgtccc ctatggcgcg gtgccgcatc
     3901 tcatgaggaa gaacgatccc taggtggttc aagatttcgt ctaatcggca tcttccccct
     3961 tgcgaagata cccatagttc caccgtgtcg caatacgcgt tccccaagcc ttctaacccg
     4021 tacttttcgg agaaatggtt cagaaaagag aggtcaaacg gcgcgttatg gaagatcaga
     4081 atcgcctctc tggggaccca cgcgaaaaag cgctccaaaa cttcctttat gccgggtttt
     4141 cctttgacca tctcattcgt aatcccggtc agttttgaaa ttgataaggg aatagagacg
     4201 cccggattca taagttcctg aaagacctcg tcctcgagaa tacgatctgc caaaacggtt
     4261 accgccgcga tctcagtgag ccgggcgcct tttcgggggt cgagtccggt cgtctccgta
     4321 tcgattgcga tgaaccgggt ggaggccgga tcgatcattc gagctcctgt atagacgtgc
     4381 gaagtagcct ttccgcaccc tcctcatcaa tcgtcgatag aaccccgata ggcgcgaaga
     4441 ggtaatgttg atcccgaaac
//
LOCUS       DAOPXN010000185         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19701_length_4449_cov_10.578289, whole genome shotgun
            sequence.
ACCESSION   DAOPXN010000185 DAOPXN010000000
VERSION     DAOPXN010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19701_length_4449_cov_10.578289"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1271
                     /locus_tag="PKM49_08730"
     CDS             105..1271
                     /locus_tag="PKM49_08730"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY82917.1"
                     /translation="MFSFRKFLSLARSSSVFSVPPVPSVRTLFFKTCQLKSWRSQGTR
                     DVANTQAFLETYLPPEVLQTIDVGTIQAEKDSFIEQDLKEFYADLLFRANIRNREAYV
                     YILLEHKSYSDYNVGVQLLRYMSAIWDKEIKKQKNRRLPLILPIVLYQGKEKWGVSTQ
                     FADRIEGVETMEGSLKKAIPHFEYYLYDFSSNSGEEIKGPDDLRLYLETIRMASIKDP
                     EKFKEAYVRITTVFVTREETEISEGLYETFVHYIYYLTAREDFLRLIQLTRTVSVERS
                     ERMQTIAEWLKQEGMEKGMEKGLIKGREEGRIEGRVEGRIEGRIEGRIEGREEGREEG
                     REELLWKQISKKFPQIPSRYFEKLKALTIDQLDTLGLDLIDMQSEEELKRHLPI"
     gene            complement(2090..3325)
                     /locus_tag="PKM49_08735"
     CDS             complement(2090..3325)
                     /locus_tag="PKM49_08735"
                     /inference="COORDINATES: protein motif:HMM:NF022376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HNY82918.1"
                     /translation="MATFEGEIVRALRNGSDLPFGAACRILKGRDREVDILRKDIQFV
                     AAGGSALRVWVGDYGTGKSLLGRAALELGLEAGLLPIYVSEPPLSKPVELYQAIINGI
                     ITPEFAGDSLFYLLSQWAESAIREIEQTGLTRGQDTLFRESVTRKILDFEFTNDFNMA
                     ISAFVDAHLSGMEDQKKKIVDWMSGRKVNYWDLKALKISRRVETKEESYLFIQDILKL
                     SRTIAYNGVLMVFDELELMQNLPSRTTFKGYEALREIVDMISSKDFPRLYSILLGTPT
                     WFENPDRGVKSYLALYDRLKTEVSVTTESTVQTLALISPDIFDPLLSDLSAIYATAYG
                     YDPKKTLTATLKEALKTRLSSPFHQNQFVEMRQVVKSLIEFFDLLKDGASAEKALQII
                     DSSARPAKEAGTQTADFWG"
     gene            complement(3325..4053)
                     /locus_tag="PKM49_08740"
     CDS             complement(3325..4053)
                     /locus_tag="PKM49_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82919.1"
                     /translation="MDVAAIVLSALTCFFILMGNFKISRLGEEQEKRFQETEHATGKL
                     LQELQNLRSEWYDKAKELSLELSKAKTEIGDLTMGKSNVSTVSDRVDFEDGIRRLDKR
                     LDSLEQTIKSLEGTIGPLVIRPLSQEKRTPVGSFDTVIPKISLVDKIKQKYPMLSTDE
                     KLFLNTLFRKTECEWHKTEDLGLDASTAKFLVPMVGRKLVFSGVPLIKIKDINGTMKV
                     LWGEGLRGEDVAGIAEAFVNEVHV"
     gene            complement(4264..>4449)
                     /locus_tag="PKM49_08745"
     CDS             complement(4264..>4449)
                     /locus_tag="PKM49_08745"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82920.1"
                     /translation="EDRYDLTAAFYSVMSEGNPAEKFYYTLTTAGVFQVPPAVLYEMY
                     DPTRVFVTDGYTVQIQE"
BASE COUNT         1082 a         1102 c         1197 g         1068 t
ORIGIN      
        1 ttatcgcggg gtaaccagcg ggctgacttc ggaagcccac gaagtctcgc gatccgcctt
       61 ttgctctttt gatccgccct gggaacgctg aactttagtt cggcatgttt tctttcagga
      121 aatttctatc ccttgcccgg tcttcatctg tgttctctgt gccccccgtg ccttcagtgc
      181 gaaccctgtt tttcaagaca tgccaactta aaagttggcg ttcccagggc acgagggatg
      241 ttgcaaatac ccaagcgttc ttagagactt atcttccccc tgaagtctta cagactatcg
      301 atgtcggcac gatacaggcg gaaaaggaca gcttcataga acaagacctg aaggagttct
      361 acgcggattt attgtttcgg gcgaacatac gaaaccgaga agcctacgtg tacattctac
      421 tggagcacaa gagctatagc gactataacg taggtgtgca gctgctcagg tatatgtctg
      481 cgatatggga caaagaaata aagaaacaaa agaacagacg actaccgctg atcttaccga
      541 tagtgctgta ccaagggaaa gagaagtggg gagtgtccac gcagtttgcg gacaggatcg
      601 aaggggtcga aacgatggaa gggtctttga aaaaagctat tccccacttt gagtattacc
      661 tatacgattt ttcgtcaaat agtggagagg agatcaaagg gccggacgat ctacgcctgt
      721 atctggagac gatcaggatg gcctcgatca aggaccccga aaagttcaaa gaagcgtacg
      781 tcagaatcac caccgtcttc gtcacgcggg aagaaacgga gataagcgaa gggctttacg
      841 agacctttgt tcactatatc tactatttga ccgcgcgaga ggatttcttg agactgatac
      901 aactgacgcg aacggtatcg gtagaaagga gtgaaaggat gcaaacgata gcggaatggc
      961 ttaagcaaga aggaatggaa aaaggaatgg agaagggatt aataaaaggc cgagaagaag
     1021 gtcggataga aggccgggta gaaggtcgga tagaaggccg gatagaaggt cggatagaag
     1081 gtagagagga aggtagagaa gaaggcaggg aagaacttct ttggaagcag atttccaaaa
     1141 aattcccaca aatcccttca aggtacttcg aaaagcttaa agcgctcacg atagaccaac
     1201 tcgatacctt gggcttggac ctgatagata tgcaaagcga ggaggaactg aaaaggcatc
     1261 ttccgatatg agaagctttg agggtcaaac agagaacacc tttccgggag cgctctgtga
     1321 ggccttaatt gtttacgccg cgatgcctgc gaagtctcgc gacctgcctt ttgccttttc
     1381 aaaaccaaaa gaagtttcgc acagagacac acggagtaca cagagagaac acagagaaaa
     1441 caataaccag atgatgactc tcgtaaaaat ctctctcctt tgtgctctcc gtgcgaaccc
     1501 atcttttttc tttcaaagca taccaactta aaagttggcg ttcccaggcg tatcgcgccg
     1561 tcgcgaaaag aaagggcgct tacgccgcga tgcctgttct ctctgtgcgc accgggtttt
     1621 ttgtgaaagt actgggaacg ccggacttta gtccggcatg tttttttctc ctttcctcaa
     1681 gcgcttaaga agccttaagc ccttacgccg tgatgcctac cgtgtggggt atcgcggggt
     1741 cccgttggtt accccacgat gacctgtgtt ctctgtgcga accgtgtttt caagacatgc
     1801 caacttaaaa gttggcgttc ccagaagaca tgccaaacta aagtttggcg ttcccaagac
     1861 gcgttcccag gcgttcgaaa gaatcttttc tccgtgttct ctgtgccctc tgtgtgaacc
     1921 cggtttttgg tctttgaaag acaggccaaa ctaaagtttg gcgttcccat agcgttcccg
     1981 ggcacaccca gacgtttcca ggcgtatcgc gccgtcgcga aaagaaaggg cgcttacgcc
     2041 gcgataacct gatcgcgcgt gacgtgtgcg agggggtatg cgagtgggct taaccccaga
     2101 agtcggccgt ttgcgtcccg gcttctttcg cggggcgcgc ggaagaatcg atgatctgga
     2161 gcgccttctc cgccgacgcg ccgtctttga gtagatcgaa gaattctatg aggcttttga
     2221 ccacttggcg catttccacg aattgatttt gatggaaagg cgagctcaaa cgggttttga
     2281 gggcttcttt caaagtggcg gtgagggtct ttttcgggtc gtatccgtag gcggttgcgt
     2341 agatcgcgga cagatccgag agaagcgggt cgaagatatc gggagagatg agcgccagcg
     2401 tctgaaccgt ggactcggtg gtgaccgaaa cttcggtttt cagccggtcg tagagcgcca
     2461 ggtagctctt gaccccgcgg tcggggtttt caaaccacgt gggcgttccg agcaagatgg
     2521 agtataggcg gggaaagtct ttcgacgaga tcatatccac gatctcgcgg agggcttcat
     2581 accctttgaa ggtggttcgc gagggcaggt tttgcatcag ctcgagttcg tcgaagacca
     2641 tgaggacgcc gttgtaggcg atggtccgcg acagcttcaa tatgtcttgg atgaagaggt
     2701 agctctcctc tttggtctcg actcgtcggc taattttgag cgctttcaga tcccaatagt
     2761 tcaccttccg accggacatc cagtcgacga tcttcttctt ttgatcctcc ataccggata
     2821 ggtgcgcgtc tacgaaggcc gagatcgcca tgttgaaatc gttcgtaaat tcgaaatcta
     2881 atatttttcg cgtgacactt tccctgaata gcgtgtcttg acccctagtg aggcccgtct
     2941 gctcgatctc tcggatcgcg ctttccgccc actgagagag gagataaaac aaggaatcgc
     3001 ccgcgaattc gggcgtgatg atgccgttga tgatggcttg gtacagttcg acaggtttgg
     3061 agaggggggg ctccgaaacg tatataggta ggaggcccgc ttcgagtcct aactctaatg
     3121 ccgcccttcc gagcagcgac ttccccgtgc cgtaatcgcc aacccatacg cgtaacgcgc
     3181 tccctccggc ggcgacgaac tggatgtctt ttcgaaggat gtccacttcg cgatcccgcc
     3241 ccttcaggat gcggcaggcg gcgccgaaag gaagatccga accgtttcgg agcgcgcgga
     3301 cgatctctcc ttcaaacgtg gccattaaac gtgaacctca ttcacgaagg cttcggcgat
     3361 accggcaacg tcctctcccc gcaatccttc tccccataaa actttcatcg ttccattgat
     3421 gtcctttatt tttatcagag gcaccccgga gaagaccagc ttccgaccga ccatcggcac
     3481 caggaatttg gccgtggagg cgtctaagcc gaggtcttct gtcttatgcc actcgcattc
     3541 cgtctttcgg aagagggtgt tgagaaacag tttttcgtcg gtcgacaaca tcgggtattt
     3601 ttgtttgatt ttatccacca aagagatctt cggtatcacg gtatcgaaag aaccgacggg
     3661 cgtcctcttt tcttgcgaca aaggccgaat cacgagcggg ccgatggttc cttccagcga
     3721 tttgatcgtt tgctcgagcg aatccaaccg cttgtcgagc cgacggatcc cgtcttcgaa
     3781 atcgacacgg tcactaacgg tagaaacgtt cgattttccc atcgtgagat cgcctatctc
     3841 ggtcttcgcc ttagaaagct ctaagctgag ctctttggct ttgtcatacc attcgcttcg
     3901 aagattttgc agttcttgca ggagttttcc ggtagcgtgt tcggtttcct gaaagcgttt
     3961 ttcttgctct tctcccaaac gggagatctt gaagttcccc atcagaataa aaaaacaagt
     4021 taaagcggaa aggacgatag cggcgacatc catcttctga acccctcctt agtgattctc
     4081 acaaacgaac ccgataagat ataagcacct ctatcatatc ataaagagga gagaagtgtg
     4141 aagaatggcc aaaaaccgta acgcgtgacg cgcgagggtc gtgaatccgg catacgcgcc
     4201 ccgatgggat acaaaaaacc gctccaaagg tagggccttc ggagcggtgt cggtaagatc
     4261 gcgttattct tggatttgga ccgtgtatcc gtctgtgacg aagacgcgcg tggggtcgta
     4321 catctcatac aacacggccg ggggtacctg gaaaacgccg gcggtggtga gcgtgtagta
     4381 aaacttctct gccgggttcc cttcgctcat cacactgtag aatgcggccg tgaggtcgta
     4441 acggtcctc
//
LOCUS       DAOPXN010000186         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19722_length_4446_cov_5.263721, whole genome shotgun sequence.
ACCESSION   DAOPXN010000186 DAOPXN010000000
VERSION     DAOPXN010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19722_length_4446_cov_5.263721"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKM49_08750"
     CDS             complement(<1..589)
                     /locus_tag="PKM49_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82921.1"
                     /translation="MGKGVRSGLIIVFLAIVCVLLFDFIDRRREMRLFFVFPQELNFR
                     FTPAKPFLFSTLEGYFEDSGAKVDVIARTYRTGEAVSPLLEAFAKDAFPKVLYWFGDP
                     SELQNLGKMEASALVFSLYPVPLQLLAAHPQICSPYGSMESFTLAVRSLVESEKVGHL
                     KIIVDSHHSFLGERVLEGLGVDFQWVPVEEAVKWSL"
     gene            complement(593..1276)
                     /locus_tag="PKM49_08755"
     CDS             complement(593..1276)
                     /locus_tag="PKM49_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNY82922.1"
                     /translation="MKTTDRKHKGERPPTERFEHYYTEKPTSSIEVRQAVFKTKGGIE
                     VAFQTPTGVFSFGHIDRASRALVEAFHFRPGSLLDIGCGYGAIGITLKKIYPAIELYM
                     SDINERAVRYAKVNMKNANVAGTVRHGAHYAPWEGYVFDTIVSNPPMAAGKAVWTELV
                     ESAPRFLKPGGSLQIVAFHRKGGERVMKTMKAAFGNMAVRVKTGGIWVYESVLATRNP
                     VEIEGGKEG"
     gene            complement(1263..2141)
                     /gene="ricT"
                     /locus_tag="PKM49_08760"
     CDS             complement(1263..2141)
                     /gene="ricT"
                     /locus_tag="PKM49_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNY82923.1"
                     /translation="MALIAKVYAVEFLPFGKLHYFSYEGELKPGDHVLGMSFFGLEAG
                     VVVWGPREIDIEEKDIEVKGLLRTFTPDDYIQYEKNLRDSDEAFHICKTKIRALGLPM
                     KLLKALYMFDRTKLLFYFSADGRVDFRELVKELAHAFKIRIELRQVGVRDELKFIGGL
                     GMCGQIVCCRRFLRDFEAVTLKDAKKQQLMINPSKISGQCRRLLCCLQYETHTYDDMM
                     KGIPGQGSIVEFENKVCKVLNSNVFTKIVTVQNDSGQTEMIPFEFFLTKKPRILIDVS
                     NVEEDNLEVLPDIEDN"
     gene            complement(2157..3332)
                     /locus_tag="PKM49_08765"
     CDS             complement(2157..3332)
                     /locus_tag="PKM49_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82924.1"
                     /translation="MGKHLIAERYADTLAYVEKTCRSLDSVSVALRCDDPYLALLILE
                     IALERFESRPWERIRNSNDCMILGETESVKIDDVRGVFHFAAMKGNDCSRKYLVFHRL
                     DELNVYAENGLLKLFEEPPSRSCVFATVSSWERLLPTVRSRMLKIEAPVRLHEATRFD
                     AVPPEWRWISKRNARFALAMLKADPARLVEILEFIRSLPLTDLLENYAALCLGNTPIV
                     FERYFKPIKNALGEEPAQQPPEREGIPEKGIDALVLREMAALLLSEKTIAHLIEEGHL
                     QPVRDWAFQQFTQTKQLWTTKYWTVFLKGFGRTLWALFYDCYRHEISMDWEKIGYPTL
                     NRRWLLSEMKVSAPKLVAWMEWMSQMGEKEWTVYHPTLSLDRLVSGLETLWTVEKSQ"
     gene            complement(3335..3805)
                     /locus_tag="PKM49_08770"
     CDS             complement(3335..3805)
                     /locus_tag="PKM49_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HNY82925.1"
                     /translation="MRIEPPDNQINDKKKAGKVKRKGKRTDSSEPYEQEWTGFIDVLG
                     NIQLDQAEEEAKRSLQEVLKAGNRFSRSPTNRNFQSYRDSIKRFLRYIEKGLYRIRED
                     VGVSADFPKLYMVADIVDEKMHQIASLLMKNEKNTLQFAGKVEEINGLILDLYR"
     gene            3920..4105
                     /locus_tag="PKM49_08775"
     CDS             3920..4105
                     /locus_tag="PKM49_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82926.1"
                     /translation="MDFVWGILVGLFLSTCIHWLFLKKQKAEAAKDKMGDFHQLSLKV
                     KDMEIRVRSLEDQVWKT"
     gene            4248..4433
                     /locus_tag="PKM49_08780"
                     /pseudo
     CDS             4248..4433
                     /locus_tag="PKM49_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
BASE COUNT         1060 a         1217 c         1039 g         1130 t
ORIGIN      
        1 cgagcgacca tttcacggct tcttcgaccg gaacccattg aaagtccaca ccgagacctt
       61 cgagaacgcg ttcgcccaaa aagctatgat gagaatccac gatgattttt agatgcccga
      121 ccttctcgct ctccacaagg cttcgtaccg ccagggtaaa ggactccatc gatccgtaag
      181 gcgaacatat ttgcggatga gcggccagta actgaagggg aaccggatat agggaaaaaa
      241 ccaaagcgga agcctccatt tttccaaggt tttgcagttc gctaggatcc ccaaaccaat
      301 agaggacctt cgggaacgcg tccttggcaa acgcctccaa caggggactc acggcttcgc
      361 cagtccgata agtccgcgcg atcacatcga ctttcgcgcc gctatcctca aaatagccct
      421 ccaatgtaga aaataggaag ggtttcgccg gagtgaatcg gaaattgagt tcttggggga
      481 agacgaaaaa tagccgcatc tcgcggcggc gatcgatgaa gtcgaacagc aagacgcaaa
      541 cgatagccag gaacacgatg atcaacccgc tccgaactcc ctttcccacc tctcaacctt
      601 cctttccgcc ctcgatctct accggatttc gagtggccaa aacgctttcg tacacccaga
      661 tacctccggt tttcacccga acggccatat ttccaaaagc cgctttcatc gtcttcatca
      721 ctctttctcc gcctttgcgg tgaaaagcca cgatttgtaa ggaacctccc ggtttcaaaa
      781 atcggggcgc gctttcgacc aactccgtcc agaccgcttt tcccgccgcc ataggtggat
      841 tcgaaacgat ggtgtcgaag acgtagcctt cccacggcgc gtaatgagcg ccgtggcgca
      901 ccgttcccgc gacgttggca ttcttcatat tcactttcgc gtaccgcacg gcgcgctcat
      961 tgatatcgct catatagagt tcgatagcgg gatagatctt tttaagcgtg atgcctatcg
     1021 cgccgtaccc acaaccaata tccaatagag aacccgggcg aaagtggaag gcttccacga
     1081 gcgcccggct cgcccgatcg atatgcccga aagagaagac gccggtgggt gtttgaaaag
     1141 cgacctcgat gccgcccttc gtcttgaaca ccgcctgccg cacctctatc gaagaggtag
     1201 gtttttccgt atagtaatgc tcgaaacgtt cggttggcgg cctttctcct ttgtgtttcc
     1261 tatcagttgt cttcaatatc cggtaggacc tccagattgt cttcttcgac attggacaca
     1321 tcgatcagta ttctcggttt cttcgtcaga aagaattcga aagggatcat ctccgtttgc
     1381 ccggaatcgt tctgcaccgt cacgatcttc gtgaaaacgt tgctgtttaa gactttacaa
     1441 actttgttct cgaattctac gatgctgcct tggccgggta tgcccttcat catatcgtca
     1501 taggtgtggg tctcgtattg taggcaacat aacagccttc ggcactggcc cgatatcttg
     1561 gacggattga tcatcagctg ctgttttttc gcgtctttga gggtcacggc ttcgaaatct
     1621 ctcaaaaaac gccgacagca aacaatctgt ccgcacatgc caagcccgcc gataaacttc
     1681 agctcatctc gaacccccac ttgacgcagc tctatccgaa tcttaaaagc gtgggccaac
     1741 tctttcacca gctcccggaa atcgactcgg ccatccgcac tgaaatagaa cagtaacttg
     1801 gttctgtcaa acatatacaa ggctttcaag agcttcatcg gtaaaccgag cgcgcgtatc
     1861 ttggtcttgc atatatgaaa agcctcgtca gaatcgcgaa ggtttttttc gtattgtata
     1921 tagtcatccg gcgtaaaggt cctcagtaac cccttcactt cgatgtcctt ctcttcgatg
     1981 tcgatctctc tgggacccca caccaccacg ccagcctcta atccaaagaa actcataccc
     2041 agtacgtggt ctcccggttt tagctctccc tcataagaaa agtagtggag tttaccgaaa
     2101 ggcaaaaact ccaccgcgta tacttttgcg atcaacgcca tctctatccc tctcgttcat
     2161 tgtgattttt cgaccgtcca tagtgtttcc aaacccgata ccagtcggtc gagactgagc
     2221 gtcggatggt aaacggtcca ttccttctct cccatctggc tcatccactc catccacgcg
     2281 actaatttcg gcgccgacac cttcatctca gagagaagcc agcgccgatt cagcgtcgga
     2341 tacccgattt tttcccaatc catcgaaatt tcgtgccgat aacagtcgta gaagagcgcc
     2401 cagagggttc tcccaaagcc tttcaaaaac acggtccaat acttagtcgt ccacagttgt
     2461 ttagtctggg tgaattgttg gaaagcccaa tctcgcacag gctgcaggtg accttcttct
     2521 atgagatggg cgatggtctt ttccgaaagg aggagcgcgg ccatctcccg caaaacgagc
     2581 gcgtcaatcc ccttttcagg tattccttct cgctcgggcg gctgttgggc gggctcttct
     2641 cccaaagcgt tttttatcgg tttgaaatac cgttcgaaaa cgatcggggt gttacctaaa
     2701 cataaggcgg cgtagttttc cagcagatcg gtcaggggca ggcttcttat gaactccaag
     2761 atttcgacga gacgggccgg atccgccttc agcatcgcca acgcaaatct cgcgtttcgt
     2821 ttcgaaatcc aacgccattc cggaggaacg gcatcaaaac gcgtcgcttc gtgaaggcgt
     2881 acgggggctt cgattttgag catccggctc cggaccgtgg gtaacaagcg ctcccaagaa
     2941 gacacggtcg caaagacgca gcttcgggaa ggaggctctt caaacagctt cagcaacccg
     3001 ttttccgcgt atacattcag ctcgtccagt ctatgaaaca cgaggtattt acgcgaacag
     3061 tcgtttcctt tcatagccgc aaaatggaag acgccccgca catcgtcgat cttcacgctc
     3121 tccgtctctc ccaggatcat acaatcgttg ctgttccgta tcctttccca ggggcgagac
     3181 tcaaacctct ccaaggcgat ctcgagtatg agaagggcca agtacggatc gtcacaccgc
     3241 aaggcaacag aaacactgtc gagcgaccgg caggtcttct cgacgtaggc caacgtatcg
     3301 gcataccgtt ccgcgatcag atgcttcccc atagttatcg gtacaagtct aggatcagtc
     3361 cgttgatttc ttccaccttt ccggcgaatt gaagggtgtt tttctcattt ttcatcaaaa
     3421 gagaggcgat ctggtgcatc ttttcgtcca cgatatccgc caccatatac agcttgggga
     3481 aatccgccga aactcctaca tcttccctga tacggtaaag gcctttttcg atatagcgca
     3541 aaaacctctt tatcgaatct cgataacttt gaaaattgcg attcgtagga gagcgtgaaa
     3601 accggtttcc cgctttcaac acctcttgaa ggcttctttt cgcctcttct tcggcctggt
     3661 cgagctggat gttcccaaga acatcgataa atccggtcca ttcttgctca tagggttcgc
     3721 tggaatcggt gcgttttcct tttcgcttga ccttccccgc tttcttttta tcgttgattt
     3781 gattatccgg tggttcgatt ctcatccttt tcgctcctca agtacgggat tatatcatta
     3841 ttatcggcgt gatcctaacg atccgtatgt ttcccgccgg ttcgtggtac aattgggtaa
     3901 cttcttcgag gtgatgacga tggattttgt ttggggaatc ctagtcggat tgtttctatc
     3961 cacgtgcata cactggctgt ttctaaaaaa acagaaggcg gaagcggcta aggataaaat
     4021 gggcgatttc catcagcttt cactgaaggt gaaagatatg gagattcgag tccgttcgct
     4081 cgaggatcaa gtctggaaga cttaaaccgc cgactcataa tgggtttttg ggtaagccgt
     4141 ggatcaggct cgggtaacat aaaatcttgc ataatgaccg cttttactcg cgcgggtcaa
     4201 gaacgagtaa aggctatttt tcatttggct atttgaaagg agagaagatg agcaaagaaa
     4261 aataccctga attttctctg gatctgattc gggtcaccga agccgcagcg atgatgagca
     4321 gtctgtacac cggtttcggc gacaaagaag cggtcgataa agccgcagtc gacgctttgc
     4381 gtgggatgct ggactatatc aacatccgcg gcaccgttgt gatcggcgaa gggtaaaaag
     4441 acgagg
//
LOCUS       DAOPXN010000187         4442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19748_length_4442_cov_4.896968, whole genome shotgun sequence.
ACCESSION   DAOPXN010000187 DAOPXN010000000
VERSION     DAOPXN010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19748_length_4442_cov_4.896968"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..814)
                     /locus_tag="PKM49_08785"
     CDS             complement(17..814)
                     /locus_tag="PKM49_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNY82927.1"
                     /translation="MTRFSEALVRANTELDPSIREHLERYQGPFADILKENERIAREK
                     RLPLCQDTGLVECFCTLGQSVALEEPLQRTLEAAVANTYGAFPFRDSTVAEPLFRRTP
                     LKDNVPPVVHLFQTEGKMLEIRFLVKGGGSENLSRYYALEPSAGVEEVENKVIELVGD
                     NGAMSCPPLHIGIGIGGFSEKAMLNAKLALTYPLGTRHKHPDYAQLEERLLRKLNTLT
                     IGFQGLGVGPTALSVHTVYEPTHIATLPLAIAVDCYLNRKGVVRFED"
     gene            complement(872..2302)
                     /locus_tag="PKM49_08790"
     CDS             complement(872..2302)
                     /locus_tag="PKM49_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HNY82928.1"
                     /translation="MKLSKIYDTLVLNRTYEHFLSYLKTPFNDVNKAHLIMLRETGIL
                     SEEIVKSIKDGLIRYDAWQGFPEKKPAEVEDYFFYAEQTLSQFIGTTAAGSLHIARSR
                     NDMDTTLFRMVMREDIAVWMLRALRLLEALIAHGRQHLATPVVLFTHGQPAQISSYAH
                     YLGAMVEEMAEVVELQHQAYEIVNRCPMGAGAITTTGFPIDRNRVATLLGFFEPIRNS
                     YQAISTSHGFLVPIQNWIVFLNDLTRFIEDVLHKSSFEVGILSYPDDLVQVSSMMPQK
                     RNPVILEHIRIKAGMAMGVFQSVVHLFHNTAYQDINENGDLTLDRFQKAFIEMMEAID
                     LFEEAMLKARVQTARVEEIAANSGITVTELADEIVRREKIAFRDAHHIVSRYVWQGAS
                     YEELRRGFETKTGKALGMNEWEVKEALAPSHFIRVRTALGGPGAMEPVFESLSRQIAN
                     AKKRVTQERERLQESRTTLLETFGRL"
     gene            complement(2299..3192)
                     /locus_tag="PKM49_08795"
     CDS             complement(2299..3192)
                     /locus_tag="PKM49_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY82929.1"
                     /translation="MRLIDSHAHFPFNEPVTAATDRVKDPRLTAERTKWKNAWGFPDA
                     ETITDFDTLADMWKRECEKHGLEKIVFTTAGGNAQAVRLVRKHPECFIGYAHHDPGSP
                     NAVQTLEEAITQGGLKGYKILGPAISTPLDSRTLYPVWEAAQSLGIPVLIHFGILGSA
                     GGIAAHPNINPLILHDVAKAFPRIPFIIPHFGCGYLFETLNLCWACPNVSIDTSGSNQ
                     WMRWMPYEMSLEMLFRKYRETIGPDRILFGSDSSSFPRGFVRRYLDDQIRAMVYVGFT
                     EAEVDRVLYLNAKTLWDTEGL"
     gene            complement(3198..4019)
                     /locus_tag="PKM49_08800"
     CDS             complement(3198..4019)
                     /locus_tag="PKM49_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY82930.1"
                     /translation="MTRSDRLVRILSYITLTALIVVMVFPFLWMASSSFKSTDEIYSL
                     TYFPNKPILDNYREIFGSSLFLRWLLNSAIIAVITTISVLFFDSLIGFTLAKYSFPGK
                     RIIFVLILSTLMIPTEMLVIPWYIMSSRLGWLDSYWGIMFPGMMSAFGVFLMRQFMES
                     VPDDLLDAARIDGMSEFGIFLRITLPLVKPALATLAIFNFIGDWNAFLWPLIVSSTPK
                     MYTLPVGLAYFSSENLMRWEMIMTGSTVSTIPLIIVFLLFQKHIVKGIALTGLKG"
     gene            complement(4031..>4442)
                     /locus_tag="PKM49_08805"
     CDS             complement(4031..>4442)
                     /locus_tag="PKM49_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY82931.1"
                     /translation="IVVTTVWVNLGYCVVIFLAGLQTIPREYLEASKIDGANRLQVLT
                     KITIPLLNPVIVFLVVTQSILFLRIFTQVYNMTDQGSGGPLNSTKPLVLYIYQKAFLS
                     FEMGAASSATVVLFGIILLITLLQFQLLKRRIQY"
BASE COUNT         1029 a         1196 c         1175 g         1042 t
ORIGIN      
        1 tccggctttg agtgtctcaa tcctcaaagc gcaccactcc tttccgattc agatagcaat
       61 cgaccgcgat cgccagcggc aaggtcgcga tatgggtggg ttcgtaaacc gtatgcacgg
      121 acaacgctgt aggcccgacg cccaatccct ggaaaccgat cgtaagggtg ttgagttttc
      181 gaagcaaacg ttcctccagt tgggcgtaat caggatgttt atgacgcgtg cccaatgggt
      241 aagtcaacgc cagtttcgcg ttgagcatgg ctttttcaga gaagccgccg atccctatac
      301 cgatgtgaag cggcggacaa ctcatcgccc cgttatcgcc caccagttcg atcaccttgt
      361 tctcgacctc ctctacgccc gcggagggtt ccaaagcgta gtagcgggaa aggttttcgc
      421 ttccgccccc tttgaccaaa aaccggatct ccagcatctt cccctccgtc tgaaaaagat
      481 gaactacagg cgggacgtta tctttcaacg gcgtccgtcg gaatagaggc tccgcgaccg
      541 tcgaatcccg gaagggaaag gcaccgtagg tgtttgccac cgcggcttcg agggttcttt
      601 gtagcggctc ttccagcgcc acgctctgtc ccaacgtgca gaaacactcc actaaacccg
      661 tgtcctgaca taaaggcaaa cgcttttctc gagcgatcct ttcattctct ttgagtatat
      721 cggcgaatgg gccttggtaa cgctcgagat gttctcgtat agacgggtcg agttcggtgt
      781 tcgcccgtac gagcgcttcg gaaaaccgag tcataatctc ctttttcgat aggatagggc
      841 tcctcctttc ttctgtgagc gtagcgtttg tttagagccg cccgaaggtt tctaaaaggg
      901 tcgttctact ctcttgcaac cgttcacgtt cttgggtgac gcgttttttt gcgttcgcga
      961 tctgacggga caagctttcg aaaacgggct ccatcgctcc cggacccccg agcgctgttc
     1021 gcacccggat gaagtgggaa ggggccagcg cttctttcac ctcccactcg ttcatcccca
     1081 acgctttccc agtcttggtt tcaaaacctc ggcgcaattc ttcgtacgac gctccctgcc
     1141 acacgtaacg actgacgata tgatgcgcgt cacggaaggc gatcttttct ctgcggacga
     1201 tctcgtcagc gagttcggtt acggtgattc cgctattagc ggcgatctct tccaccctcg
     1261 ccgtttgaac gcgcgctttg agcatcgcct cttcgaaaag atcgatggcc tccatcatct
     1321 cgatgaaggc tttttgaaac cggtccaagg tcaagtcgcc gttttcgttg atatcctggt
     1381 aggcggtatt gtgaaagaga tgcaccacgc tttggaaaac gcccatcgcc atacccgcct
     1441 tgatgcggat atgctccaat atgaccggat ttcttttttg tggcatcatc gagctcactt
     1501 gcacgagatc gtccgggtaa gacaggatgc cgacttcaaa agaagatttg tggagaacgt
     1561 cttcgataaa acgtgtcaaa tcgtttagaa acacgatcca gttctgaatt ggaaccagaa
     1621 acccgtgaga ggtcgatatg gcctgatagg agtttcggat aggctcgaaa aatccgagca
     1681 aggtggcgac ccgattccgg tcgatcggga atccggtggt cgtgatcgct ccggcgccca
     1741 tcgggcagcg gttcacgatc tcgtaggcct gatgctgcaa ctctaccacc tcagccatct
     1801 cctctaccat cgcgcctaag taatgggcgt acgatgagat ttgcgccggc tggccgtgtg
     1861 tgaacaagac gacgggggtg gcgagatgct gtctcccgtg cgcgattagc gcttccagga
     1921 gccgaagggc ccgcagcatc cacacggcga tatcttccct catcaccatg cgaaagagcg
     1981 tcgtgtccat gtcgtttctg cttcgcgcga tgtgtagaga accggcggcc gtcgtcccta
     2041 tgaattggga gagcgtctgt tccgcgtaaa agaagtaatc ttccacctca gcaggttttt
     2101 tctccggaaa gccctgccac gcgtcatagc gtatcaaccc gtccttgatg ctcttgacga
     2161 tttcttcgga aagaatgccg gtttcgcgaa gcatgatcaa gtgcgcttta ttcacatcgt
     2221 tgaacggggt ttttagataa gagaggaaat gctcgtaggt acggttcaga acgagggtat
     2281 cgtagatttt tgatagtttc atagcccctc cgtgtcccat aaggttttag cattcagata
     2341 gaggacgcga tccacttcgg cttccgtaaa cccgacgtag accatcgccc ggatttgatc
     2401 gtcgagatag cgtctgacga accccctcgg gaaactgctt gagtcggaac cgaagagtat
     2461 ccgatcgggt ccgatcgttt cccggtactt gcggaagagc atctccagac tcatctcata
     2521 aggcatccag cgcatccatt gattcgatcc gctcgtatcg atagacacgt tgggacacgc
     2581 ccagcagagg ttcaacgtct caaacaggta cccgcacccg aagtggggta tgatgaaggg
     2641 tatccgcggg aaggctttcg cgacatcgtg gaggatcaac ggattgatgt tcgggtgagc
     2701 ggcgattccg cccgcgctcc ccaaaatgcc gaaatggatc aagacgggga tgcccaatga
     2761 ttgcgccgct tcccagaccg gatacagagt tcggctgtcg aggggtgtgg aaatcgcggg
     2821 gccgaggatc ttgtaccctt tcagcccgcc ctgcgtgatc gcttcctcca aggtctgcac
     2881 cgcgtttggc gagcctgggt cgtgatgggc gtacccgatg aagcactccg gatgcttacg
     2941 taccaggcga acggcttgcg cgttccctcc ggccgtcgtg aaaacgatct tctccaatcc
     3001 gtgtttttca cactctcgct tccacatgtc cgccaacgtg tcaaaatccg tgatcgtttc
     3061 agcatccgga aatccccagg cgtttttcca cttcgtccgt tcggctgtca atcgcgggtc
     3121 tttgacccgg tctgtggccg ccgtcaccgg ttcgttaaac ggaaaatgtg cgtgagagtc
     3181 tatcaatcgc atcgctatca ccctttcaag cccgtgaggg cgattccttt gacgatatgc
     3241 ttttggaaca agaggaacac gatgatcaac gggatcgtcg aaaccgttga cccagtcata
     3301 atcatctccc agcgcatcaa attctcgctt gaaaaatagg ccagacctac gggcaacgta
     3361 tacattttgg gcgtgctaga aacgatcagc ggccaaagaa aggcgttcca gtcgcctatg
     3421 aagttaaaga tcgccagcgt cgcgagcgcg ggtttgacca gcggtaaggt aattctcaag
     3481 aatatcccaa actcagacat cccatctatt cgcgcggcgt ccagtagatc gtccggcacc
     3541 gattccataa actgtcgcat cagaaagacg ccgaacgcgc tcatcattcc ggggaacata
     3601 atgccccaat acgaatccag ccaacctaaa cggctggaca taatgtacca cgggattacc
     3661 agcatttcgg tcggaatcat aagggtgctg aggatgagaa cgaagatgat tcttttgcca
     3721 ggaaatgagt atttggcgag ggtgaagccg atcaacgagt cgaaaaacag aacggagatt
     3781 gtcgtaatca ccgcgatgat cgcgctgttc aagagccaac ggagaaatag ggaagagccg
     3841 aagatctcac gatagttgtc gaggataggt ttgttgggga aataagttag gctatagatc
     3901 tcgtcggtag atttgaaaga tgaggaggcc atccataaga atgggaagac cataacgaca
     3961 atcaaagctg ttaatgtgat gtacgagagg atccgcacca accgatcaga acgcgtcatc
     4021 tctctgcccc ctagtattga attctccgct taagcagctg gaactgcaaa agcgtgatca
     4081 ataggataat gccgaacagc accaccgtgg cgctcgaggc tgctcccatc tcgaaggata
     4141 aaaaggcctt ttgatagata tacaaaacca gcggcttggt tgaatttaga ggacctccgg
     4201 agccttgatc ggtcatattg tatacctgag tgaagattcg taggaagagg atgctttgcg
     4261 tgacgaccaa gaacacgatc accggattga gaagcgggat cgtgatcttc gtcagaacct
     4321 gcagtcgatt cgcgccgtct attttcgagg cctccaggta ctctctcggt atcgtctgta
     4381 gtccggcgag gaagatgacg acgcagtatc cgaggttgac ccaaaccgtt gtcacgacga
     4441 tc
//
LOCUS       DAOPXN010000188         4434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_19797_length_4434_cov_4.649007, whole genome shotgun sequence.
ACCESSION   DAOPXN010000188 DAOPXN010000000
VERSION     DAOPXN010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19797_length_4434_cov_4.649007"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /gene="rplF"
                     /locus_tag="PKM49_08810"
     CDS             <1..341
                     /gene="rplF"
                     /locus_tag="PKM49_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNY82932.1"
                     /translation="GTFFALIRNMVLGVTEGFQKELHVVGVGYKAAMKGKNLEMDLGY
                     SHSVVVVPPDGITFSVPAPNKIIVKGADKEQVGQVAANIRKWREPMVYGGKGIRYSDE
                     TVVVKVGKKV"
     gene            366..734
                     /gene="rplR"
                     /locus_tag="PKM49_08815"
     CDS             366..734
                     /gene="rplR"
                     /locus_tag="PKM49_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNY82933.1"
                     /translation="MLTKTDRRVMRKRRHIRVRKKINGTGSKPRMAVFRSEKHIYVQL
                     IDDTQGVTLLSASSREILVSGEVKKTWNKEAAKIVGKKIAEKALEKGIAEVTFDRGGY
                     RYHGRIKELADGARAAGLKF"
     gene            749..1285
                     /gene="rpsE"
                     /locus_tag="PKM49_08820"
     CDS             749..1285
                     /gene="rpsE"
                     /locus_tag="PKM49_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNY82934.1"
                     /translation="MRQEELKDTFQKEEFELEERVVEIRRTTKVVAGGKNLHFRVLAV
                     VGDKNGKVGIGIGSAREVPESIRKAIAEAKKNMVQVSIVKGTIPHEVMVKFCSAKIML
                     KPAAPGTGIVASNAVRAVVELAGVKNILTKNLGSTAPVNVIKATFEGLKSLRSPSQIA
                     SKRDLTLREVFHGAGNKG"
     gene            1292..1525
                     /gene="rpmD"
                     /locus_tag="PKM49_08825"
     CDS             1292..1525
                     /gene="rpmD"
                     /locus_tag="PKM49_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNY82935.1"
                     /translation="MEHVKITLIKSPIGYAKNQKLTVRSLGLRKMNDSVIHVKNDAIM
                     GMANTVRHLVKVEAVEFEERLSENLSSREEEIE"
     gene            1522..1974
                     /gene="rplO"
                     /locus_tag="PKM49_08830"
     CDS             1522..1974
                     /gene="rplO"
                     /locus_tag="PKM49_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012002645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNY82936.1"
                     /translation="MTLKIEDLTPTPGSMKKRRRVGRGPGSGMGKTSTRGHKGQGSRS
                     GRGKIGAGFEGGQTPLFMRSPKRGFKNFTKKEYVIVNLEDLDTRFQANEEVTPKTLLE
                     KGLIHKVKDGIKILGVGQLSKALTVKASQFSASAKEKIVAAGGSAEVI"
     gene            1975..3264
                     /gene="secY"
                     /locus_tag="PKM49_08835"
     CDS             1975..3264
                     /gene="secY"
                     /locus_tag="PKM49_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNY82937.1"
                     /translation="MWRAFRNAFRIPELRDRIIFTLLMLIVYRLGIYIPIPGINLSEW
                     ANVFQGFQSGAAGGLFSFYDVFAGGAVERFSILMMSVTPYINASIILQLFMSIIPSLK
                     ELSKEGEEGKKKLEKYTRYLTLFLGFLQGFILSVSISRNPALIVEGISPFFFMFVSTL
                     TIVAGTMFAIWLGERITEKGIGNGISVLIFAGIVSRYPSYVGQALIGQMGIMEWIILL
                     AVIAAIIIAIIYTQLGERRVTVQYARRVTGRKVYGGQTTYIPIKVNQAGVIPIIFGQA
                     IMVIPSAIAQLSGSEVVKRWLGTNGYLYLPIYAILILFFTYFYSMATFDVHDVSENIK
                     KYGGFVPGIRPGAPTEKYFTRILNRMTFIGAVFLVIMALVPYLLSSTLGVNIWIGGTS
                     ALIAVGVALDILQQMEAHMIMRNYEGFMKKGRLKGRA"
     gene            3266..3970
                     /locus_tag="PKM49_08840"
     CDS             3266..3970
                     /locus_tag="PKM49_08840"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNY82938.1"
                     /translation="MPNRQKLTYHTIFLGAPGSGKGTAASRLGAYLRIPHISTGDMLR
                     ESVASSTDLGTRVKAVMEQGLLVNDEIMRELVKDRLQRADCRKGFILDGFPRTIAQGK
                     ALDEILAESHKRLDFVFVMEIEENALVRRLSNRRICPQCGRVYNLIGLKPRQEGLCDD
                     HPVALIQREDDREETIRKRMTVYRENTLPLIEYYRNRYLIVTVNSDATIDKTVEVVVN
                     TVLERDSTLRKKEEEV"
     gene            3967..>4434
                     /locus_tag="PKM49_08845"
     CDS             3967..>4434
                     /locus_tag="PKM49_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNY82939.1"
                     /translation="MIILKRPEEIEKIAVSGRIVGLAIQLAGERLRPGVTTREIDIWI
                     EEFIRSQEATPSFKGYRGYPYASCISLNEEVVHGMPAKNRRIKEGDIVSIDVGAFKDG
                     YHADAARTFAIGEVSFEAKKLMEITFQALDKAIEQVTPKKPLGAVSHAIQAWVE"
BASE COUNT         1126 a         1082 c         1293 g          933 t
ORIGIN      
        1 ccgggacgtt tttcgctctc atccgcaata tggtcctcgg cgtcacggaa gggtttcaga
       61 aggagctgca cgtcgtcggc gtcgggtaca aagctgcgat gaaaggaaag aacctggaaa
      121 tggacttggg ctattcgcac tcagtcgtcg tcgtgccgcc cgacggtatc acctttagtg
      181 tgccggcccc gaacaaaatc atcgtcaagg gggccgacaa ggaacaggtc ggacaggtcg
      241 ccgcgaacat acgcaagtgg agagagccga tggtctatgg tgggaaaggg attcgatact
      301 ccgacgaaac ggttgtcgtt aaagtcggta agaaggttta gggacgatga agggaggttg
      361 tggttgtgtt gacgaagact gatcgacgcg tgatgcgcaa aaggcggcat atccgcgttc
      421 gaaaaaaaat aaatggaacg ggctcgaaac ccagaatggc agtttttcgg agcgaaaagc
      481 acatttacgt gcagttgatc gacgacaccc aaggtgtgac gctcctgtct gcctcttcac
      541 gggaaatcct agtttcgggt gaagtgaaaa aaacttggaa caaggaagcg gccaaaatcg
      601 tagggaaaaa gatcgctgaa aaagcgttgg aaaagggtat cgcggaagtc accttcgacc
      661 gaggcggata ccgataccac ggtcggatca aggaactggc agacggcgcc cgcgcggcgg
      721 ggcttaagtt ttagcaggag ggatgagagt gcggcaggaa gaactgaaag acacgttcca
      781 gaaagaggag ttcgaactcg aagaacgtgt cgtggaaatc cgacggacga ccaaggtcgt
      841 agccggcgga aagaacttgc attttcgggt tttggccgtt gtcggagaca aaaacggcaa
      901 ggtcggaatc gggatcggat ccgcccgaga agtgccggag tccatccgaa aggccatcgc
      961 cgaagcgaag aagaacatgg ttcaggtgtc gatcgtcaaa ggaacgattc ctcacgaggt
     1021 gatggtaaag ttttgttcgg cgaaaatcat gctgaaaccg gcggctcccg gaacgggtat
     1081 cgtagcttcg aacgccgttc gagcggtcgt tgaactggcg ggcgtcaaaa atatcctgac
     1141 gaaaaacttg ggctcgaccg ccccggtcaa cgtcatcaaa gccaccttcg aaggtttgaa
     1201 atccctccgg tccccctctc agatcgcgag caagagagac ttgactctga gggaagtctt
     1261 ccacggcgcc ggcaacaagg ggtgattcaa aatggaacac gtgaagatta ctttgattaa
     1321 aagtccgatc gggtacgcca agaaccagaa gttgaccgtt cgctcgctgg gtttacggaa
     1381 gatgaacgat tcggtcatcc acgtcaagaa tgacgccatt atgggaatgg cgaacaccgt
     1441 gcggcacctc gtgaaggtgg aagccgtcga attcgaagaa cggctttccg aaaacctatc
     1501 gtccagagag gaggaaatag aatgaccctt aagatagaag atttgacccc cactcccggc
     1561 tcgatgaaaa agcgccggcg ggtaggtcgc ggccccggat ctggaatggg aaagacatcg
     1621 acgagagggc acaaaggtca aggctcacgc tccgggaggg gcaagatcgg cgccggattc
     1681 gagggcggac agaccccgct ttttatgaga tccccgaagc gcgggttcaa gaactttacc
     1741 aaaaaggaat acgtcatcgt taatctcgaa gacttggaca cccgttttca agcgaacgag
     1801 gaagtgactc ctaaaacgtt gctcgaaaaa ggattgatcc ataaggtcaa agacggaatc
     1861 aaaatcctcg gcgtaggtca gctcagcaaa gccttgacgg ttaaagcgag tcagttcagc
     1921 gcaagcgcga aggaaaagat cgtggctgcc ggcggatccg ccgaggtgat ataaatgtgg
     1981 agagccttta gaaacgcgtt tcgcattcct gaattaaggg accgcatcat cttcactttg
     2041 ctgatgctga tcgtttaccg cttggggatc tatatcccca ttccgggtat taacctgtcc
     2101 gaatgggcaa atgtgtttca agggtttcaa tcgggcgcgg ccggcggctt gtttagtttc
     2161 tatgacgttt ttgccggcgg agccgtcgaa cggttttcca tactgatgat gagcgtgacg
     2221 ccgtatatca acgcctccat catcttgcag ctgtttatga gcatcatccc ctctttgaaa
     2281 gagctctcga aagaagggga agagggcaag aaaaagcttg agaaatacac gcgctatctg
     2341 acgctcttct tggggttcct tcaggggttc atattgagtg tgtcgatctc tcggaatccg
     2401 gcgctgatcg tggagggcat ctctcccttc ttttttatgt tcgtgtccac gttgacgatt
     2461 gtggccggga cgatgtttgc catctggttg ggtgaacgga tcaccgagaa aggcatcggc
     2521 aacggaatct ccgttctgat cttcgccggt atcgtctctc gatacccgag ctacgtcggt
     2581 caagcgctca ttgggcagat ggggattatg gagtggatta tcctcctcgc cgtgatcgcc
     2641 gccatcatca tcgctatcat ctatacccag ctcggggagc ggagggtcac cgttcagtac
     2701 gcgcggcgcg tgaccggaag aaaggtgtac ggtggtcaga cgacgtacat cccgatcaaa
     2761 gtcaaccaag cgggggttat cccgatcatc ttcggacagg cgattatggt catcccctcg
     2821 gccatcgccc agctgtccgg ctctgaagtc gtgaaaagat ggctcggcac caatgggtat
     2881 ctgtatctcc cgatctatgc gattctcatt ctgttcttca cctacttcta cagcatggcg
     2941 accttcgatg tccacgatgt gtccgagaac atcaaaaagt acggagggtt cgttccgggt
     3001 atacgcccgg gcgcgccgac ggagaagtat tttacccgca tcctcaaccg gatgaccttc
     3061 atcggggcgg tctttttagt catcatggcg ctcgtgccct acctgctctc ctcgacgctc
     3121 ggcgtgaata tctggatcgg cggaacctcc gccctcatcg ccgtaggagt cgcgctggac
     3181 atcttgcagc agatggaagc gcatatgatc atgaggaact acgaggggtt catgaagaaa
     3241 ggccgactca aaggtagagc gtaggatgcc caaccgccag aaactcacct atcatacgat
     3301 ctttttgggg gcccccggtt ccggcaaagg aacggcggcc tcccgattag gcgcctattt
     3361 gaggataccg catatctcca ccggcgatat gctccgagag tctgtggcgt cgtctacgga
     3421 cttggggacc cgtgttaaag cggtgatgga gcaggggctg ctcgtaaacg acgagattat
     3481 gagagaactc gtcaaagacc gtctccagag ggccgattgt cgcaagggat tcattttaga
     3541 cggcttcccg agaacgatcg cccaagggaa agccttggac gaaatcctgg ccgaaagcca
     3601 taagcgcctg gatttcgtct ttgtgatgga aatagaagaa aacgcgttgg tccggcgttt
     3661 gtccaatcga aggatctgcc cccaatgcgg gagagtctac aatctgatcg gattaaaacc
     3721 gcggcaggag ggcctgtgcg acgatcaccc ggtcgccttg atccagcggg aagatgatag
     3781 ggaagagacc atccgcaaac gaatgacggt ctatcgggag aacacccttc cattaatcga
     3841 atattatcgg aataggtacc tgatcgttac agtaaattct gacgccacta ttgacaaaac
     3901 cgtagaggtt gtagtaaata cggttcttga aagagatagc actttgcgaa aaaaggaaga
     3961 agaagtttga ttattttgaa gcgcccggaa gagatcgaga agatcgcggt ttccggaaga
     4021 atcgtcggcc tcgcgataca actggccggc gaacgattga gaccgggggt caccacccga
     4081 gagatcgaca tctggataga ggagttcata cggtctcagg aggctacccc gtcgtttaaa
     4141 gggtatcgcg ggtatccgta cgcctcctgc atttctttga acgaagaggt cgtgcacggt
     4201 atgccggcca agaaccgtcg gataaaagaa ggggacatcg tttcgatcga cgttggcgcc
     4261 ttcaaagacg gctatcacgc cgacgccgcc aggaccttcg caatcggaga ggtttctttc
     4321 gaggcgaaga aactgatgga gatcaccttt caagcgctag acaaagcgat agagcaggtt
     4381 acgccaaaga agcctttagg ggccgtatcg catgcgatac aagcctgggt cgaa
//
LOCUS       DAOPXN010000189         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20089_length_4388_cov_4.410339, whole genome shotgun sequence.
ACCESSION   DAOPXN010000189 DAOPXN010000000
VERSION     DAOPXN010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20089_length_4388_cov_4.410339"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PKM49_08850"
     CDS             <1..312
                     /locus_tag="PKM49_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNY82940.1"
                     /translation="HKNEDNVDMLMESTGRGKVEAIEKAGLDIIIPAFVIGELEIAFK
                     MSILLYIPFIVVDMLVASILMAMGMIMIPPALISMPFKILLFIAVNGWDLIVMGLIRS
                     F"
     gene            complement(309..3017)
                     /locus_tag="PKM49_08855"
     CDS             complement(309..3017)
                     /locus_tag="PKM49_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNY82941.1"
                     /translation="MVKPQKIIVSHKNPDFDAFSASVAAQRLFPDHVIVVSGEGAQNL
                     EDYFSLYKEKIPFFREETVDYSEVKEVVIVDASLMERLGQAVQNALALNKPVLSIYDH
                     HPVYDGDIFPGFNKTETVIAEVGSVTTMLVERIRKQNLAINKVDATLFSIGIYEDTGN
                     FLFSSTTPRDLQSSAYLLEKGANLDVVSAFVKIDMTIDQKRLLEDLLTNKVVKHINQT
                     EIAIGIANSEKFIGGLNLIVSKLWMSEGYDTFIAVVGMGKKTYIVGRTSSPDVNLGEL
                     MGALSGGGHKRAAACKLSGVPVDDAVKILSNYLERYVTPAIRAKHLMSSPVKTVFANM
                     TIQAINKIFEETGYGGLPVIEDRKLIGMIVRRDVQKAMAHLLKDKPVKSIMSTKLQVA
                     DVEDSIEKVRVMMVENGIGRIPVLKEGVLVGIISRTDLIRASYKNGHDHTRLDVELPL
                     GQYDTSVLLQRKVPLELTRLLRLIGETGDQLNIKTYVVGGFVRDLLLKIPNLDVDIVV
                     EGNAIEFVKILAARCKESPNVSRILKIEEHPPFQVSKTFFENGYTVDFASARIETYHS
                     PGALPVVEFSHIRKDLVRRDFSINAMAIDLGKDSFGALLDFFRCRRDLENGSIRVLYN
                     TSFIDDPTRILRGVRYEIRFGFTMEERTLELLKEALAEKYLERVSGGRIRREFQRSLT
                     ESSLFRLFKRYGELEILKHCFPATYYTETLEEKLKRFFDRLLWVERFYDYKGAFNRFY
                     ALLYLLLEYNTEEQLRDVEAEYGVPRKVTRDIYSFSKKVALIAHMFNTECAFSDLYVL
                     LEGAKSESLAYLCAYLTEDGTNHFKEYSRLRKGVRFHYVDGNVLTKQYHLQKGSEIRE
                     VIVKMTQEKMLGSLNNAQEERDYLEQLLGCDAQPTGLGRPVSPIGG"
     gene            complement(3053..3751)
                     /locus_tag="PKM49_08860"
     CDS             complement(3053..3751)
                     /locus_tag="PKM49_08860"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HNY82942.1"
                     /translation="MDTLTSINTRKGPEERPLLKAFLDLFFPNPCPLCNERTSAGRYL
                     CPSCEKALDELLPAPVLGKGSGKVARLHYYSVEDSPLYELIKAYKYLPRKGLADHLAL
                     FLYRLIVFWRVSVPFIIPLPAHPASIRARGFSNTRLILDRLKVRFMPEITILDPVVRR
                     EWSYRPQASLIDEAKRKDNVRGVFRVKKGTILPERVALFDDVYTTGATVAEYCRVLAP
                     LVKEIDLFLLIRRK"
     gene            complement(3774..>4388)
                     /locus_tag="PKM49_08865"
     CDS             complement(3774..>4388)
                     /locus_tag="PKM49_08865"
                     /inference="COORDINATES: protein motif:HMM:NF023413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNY82943.1"
                     /translation="LKADSFGTVEALKNAIARLQTAEISIEIVHSGIGAVSPSDVMLA
                     SASDGVILGFKVKIDKKAEIDADREKVQIKTYDIIFNLIEDVKKALLGMLEPDKVEEV
                     IGHGEVRQAFRIKRVGTIAGVQMLDGFVEKGSKVKVFRNNIEIFRGGLDSLKHFQDDV
                     QRIEAPQECGIRFAGFDDVNENDELEFFQIKEVKRKLEFIQQDK"
BASE COUNT         1003 a         1169 c         1068 g         1148 t
ORIGIN      
        1 cataagaatg aagacaacgt ggatatgctt atggagagca cgggaagagg aaaagtggaa
       61 gcgatagaaa aagcggggtt ggatatcatc attccggctt tcgttatcgg agaattagag
      121 atcgcgttca agatgtcgat ccttctgtat atccccttca tcgttgtgga tatgctggtc
      181 gctagtattc tgatggccat ggggatgatc atgattccgc cggcgctgat ctctatgccc
      241 ttcaaaatcc ttctcttcat tgcggttaac gggtgggacc tcatcgtgat gggtttgatc
      301 agaagcttct agcctccgat tggcgaaacg ggtcgaccca aaccggtcgg ttgtgcatcg
      361 catcctagta gttgttccag gtaatccctc tcttcctgcg cgttgttcag tgatccaagc
      421 atcttctcct gcgtcatctt gacgatgacc tcgcgaatct cgctcccttt ctgcaagtga
      481 tactgttttg tgagcacgtt gccgtccaca tagtgaaacc gaactccctt tcgaagccgc
      541 gaatactctt tgaaatggtt cgtcccgtct tcggtcagat acgcgcacag gtaagcgagc
      601 gactcgctct tcgcaccctc caagaggacg tacagatccg aaaacgcgca ctccgtgttg
      661 aacatgtgcg cgatcagcgc cacttttttt gaaaaggagt aaatatctcg ggtcacctta
      721 cgcggcaccc cgtattcagc ttctacgtct ctgagctgct cttccgtatt gtattccagc
      781 aacaggtaca gtaaggcata gaatcggttg aaggcgcctt tgtagtcgta gaacctttca
      841 acccataaca gtcgatcgaa aaaccgtttg agcttctctt ccaaggtttc ggtgtaatac
      901 gtcgccggaa agcagtgttt caatatctcc agttctccat accgtttaaa aagcctaaat
      961 agagaagatt cggttaaact tcgttggaat tcgcgacgga ttcttccccc gctaacgcgt
     1021 tccaagtatt tttcggccaa cgcttccttc aatagttcca gcgtccgctc ttccatcgtg
     1081 aagccgaagc ggatctcgta gcgaactccc cgtaggatcc gagtgggatc gtcgatgaag
     1141 ctggtgttat acaatacgcg gatcgaaccg ttttccaaat cccgccggca acggaaaaaa
     1201 tcgagcagcg cgccgaaaga gtctttgccc aagtcaatcg ccatcgcgtt gatgctaaaa
     1261 tcccttctaa cgaggtcttt ccgtatgtga gagaactcta cgacgggtaa agcgccgggt
     1321 gagtgatagg tttcgattcg agccgaagcg aagtccacgg tatagccatt ctcgaagaag
     1381 gttttcgaga cttggaaagg gggatgttct tctatcttaa gaatacgaga gacgttcggg
     1441 gactctttac atcgggcggc aagtattttt acgaactcga tagcgttgcc ttccaccacg
     1501 atatccacgt cgaggttcgg tatctttaaa agcaagtccc ggacgaatcc tcccaccacg
     1561 taggttttaa tgttcaactg atcgccggtt tctccgatca agcgtagaag tctcgtcagt
     1621 tctagcggca cctttcgttg caacaagacg ctggtatcat attgtccgag gggaagctcg
     1681 acgtccaatc gggtatgatc gtggccattt ttgtaggaag cgcggatcag atccgttcgc
     1741 gagatgattc ctacgagcac gccctccttc aaaaccggaa tcctaccgat cccgttctcc
     1801 accatcatca ccctcacctt ttcgatcgag tcttccacat cggccacctg gagttttgtg
     1861 gacataatgc ttttcactgg tttgtctttc aacaggtggg ccatcgcttt ttggacgtct
     1921 cggcgcacga tcataccgat caactttctg tcttcgataa ccgggagtcc cccgtatccc
     1981 gtttcttcga aaatcttgtt gatcgcttgg atcgtcatat tggcaaagac ggtcttcacg
     2041 gggctgctca tcaggtgttt cgcgcggatg gccggggtca cgtaccgctc taggtagttg
     2101 ctcaatattt tcactgcatc atcgacgggc acgccggaca acttacaagc cgccgcgcgt
     2161 ttgtgtcctc ctccgctcaa agcgcccatc aattctccca ggttgacgtc tggcgaggag
     2221 gtgcgtccta cgatataggt cttttttccc ataccgacga cagcgataaa ggtgtcgtaa
     2281 ccttcggaca tccataattt gctcacgatg agattaagtc cgccaataaa cttttcgcta
     2341 ttggcgatac cgatcgcgat ttccgtctga ttgatgtgct tgacgacctt attggtgagg
     2401 aggtcttcca acaagcgttt ttgatcgatc gtcatatcga tcttcacgaa agcggagacg
     2461 acgtccaggt ttgcgccctt ttccagaagg taggcgctcg attgcaaatc ccgtggggtc
     2521 gtcgaggaga acagaaagtt gcccgtatcc tcgtaaatcc ctatagaaaa cagcgtggca
     2581 tcgactttgt ttatggcgag gttttgtttc cggattcgtt cgaccaacat cgtcgtgacc
     2641 gagccgacct ccgctatgac cgtctccgtt ttattaaaac cgggaaagat gtctccatcg
     2701 tagaccggat ggtggtcgta aatactcaaa actggtttgt taagggcgag agcgttttgt
     2761 acagcctgtc ctaaacgctc catcaaagaa gcgtctacga tcacgacctc cttgacctcc
     2821 gaatagtcga cggtctcctc gcgaaagaac gggatttttt ctttgtataa cgagaaatag
     2881 tcttccagat tttgagctcc ctcgccggaa accacgatca cgtgatcagg aaacaacctt
     2941 tgagcggcga cggaagcgga aaacgcgtca aaatccgggt ttttatggct tacgatgatc
     3001 ttttggggct taaccaaccc gcgtttgtct tctttaccca acgtacttca catcactttc
     3061 ttcgtatgag gaggaacaag tctatttctt ttacgagcgg ggccaatacg cggcaatact
     3121 ccgcgacggt ggcgcccgtg gtgtacacgt cgtcgaagag cgcgacccgt tccggtagta
     3181 ttgtaccttt ttttacccga aagacaccac gaacgttgtc cttccgtttt gcttcgtcga
     3241 taagcgaagc ttgcggacgg taagaccatt cacgccgaac gacggggtcc aagatcgtga
     3301 tctccggcat gaagcggact ttcagacggt ccagaatcag ccgagtgttg gaaaacccac
     3361 gtgctctgat cgaggcgggg tgcgcgggta aagggatgat gaacgggacg gaaacgcgcc
     3421 agaagacgat caatcgatac aggaaaagag ccaggtggtc cgccaagcct tttctcggca
     3481 ggtatttgta ggctttgatc aattcgtaaa gcggcgaatc ctccacggaa taataatgaa
     3541 ggcgcgcgac ctttccggat cctttgccca aaaccggcgc cggtagcaat tcgtctagcg
     3601 ccttttcaca actcgggcac agataccgtc cggcgctcgt ccgttcattg cagagcgggc
     3661 acggattcgg aaaaaaaagg tccaaaaaag ctttcagcaa cggccgctct tccggacctt
     3721 tccgtgtatt gatcgaggtt agtgtatcca aacgccacct ctggccgagt ccgtcattta
     3781 tcctgctgta tgaactccaa tttccttttt acttctttaa tctggaaaaa ttccaattcg
     3841 tcgttctcgt tcacatcgtc aaaaccggcg aatcggatac cgcattcctg aggggcttcg
     3901 attcgctgaa cgtcgtcctg aaagtgcttc agagagtcga gaccgcctct gaatatttcg
     3961 atgttgttcc taaaaacctt caccttcgaa cccttctcta cgaacccgtc cagcatctgc
     4021 accccggcga tagtcccgac tctcttgatc cggaaggctt gtcgcacctc tccgtggccg
     4081 atcacctctt ctaccttatc gggctcgagc attccgagta gcgctttttt gacgtcctcg
     4141 atcagattga agatgatatc gtaagtcttg atttgcactt tttctcgatc cgcgtcgatc
     4201 tcggccttct tgtcgatctt gaccttaaac cctaagatca ccccgtccga ggcggaagcc
     4261 agcatcacgt cagatggaga aacggcgccg atcccggaat gaacgatctc tatcgatatt
     4321 tccgccgtct gcaaacgggc gatcgcgttt tttagggcct ccacggtccc aaagctgtcc
     4381 gccttgag
//
LOCUS       DAOPXN010000190         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20326_length_4349_cov_6.251281, whole genome shotgun sequence.
ACCESSION   DAOPXN010000190 DAOPXN010000000
VERSION     DAOPXN010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20326_length_4349_cov_6.251281"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1499)
                     /locus_tag="PKM49_08870"
     CDS             complement(75..1499)
                     /locus_tag="PKM49_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY82944.1"
                     /translation="MTHKSEESSQKRSAKASSRKGQWRLVVSHRVLLLLFFIGAICVG
                     FWGFYHVQDTRKKELSEARAVHCLELAGEFDKFIRNQESRIEWLLSEISALLEPGEVP
                     TTSSFVDRLETEIAPLLKSVVANCAELDACFFHLNPQIDQKYFDITYVKSADRSIRRQ
                     PSLAKEEYDKANPSMVWFYTPLSRKVPGWSEVFYLDALEDHLISYSAPVLHNGQPIAV
                     VGIFVRLRHLIERVARISDSEDSFGFLLNSERVVLYHPDYAKGVLFEHLEPVDLAVLD
                     EALSKGPTLSPGVWRDRSRLLTFAKLANGWILGFDISFGNTGVPFHAVAVFVIAIGLI
                     VLFSAFIFGYVYNRSVFKPLAILSETTTRFSQGESGARYFGPTSGPVGQLGVSFNEFA
                     DNLANRLATIRRQNEDISAINQELEAMNQQLQESYNVSEELTASLHKTIALTSRSSLS
                     TFESQHSFLKEALDTILMLLPKAD"
     gene            complement(1829..2881)
                     /locus_tag="PKM49_08875"
     CDS             complement(1829..2881)
                     /locus_tag="PKM49_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HNY82945.1"
                     /translation="MKRILFVLIFAIFTVSIFAGLSGKVAEVDKYGNTYTDLLQDSAT
                     EAGLEFGDIVELAFGDHRLVVPFVRNYGDVDRGQPLLRISGGKLLMAISYGNFSQVYA
                     VALGMGVNISLVEKGAYLGELEIRNLVRTDVRSDYVTDVVFANFRPVRMGDIAPNTLY
                     RCSHPSINDPRSPYANALVEGAGIKTVVNLSDSDEELAENYKYSEFYRSLGEAGHIIN
                     LNMGVDLLSPDFARKLRLGFLFMIENEPPYLVHCVEGKDRAGIVSALLAAIMNGEVED
                     IYRDYIVSYINYYHVKPGTPAYAAVRKIIADIFAQMNDGKPVDDSNIRFVALKYLRNQ
                     VGLSLEEIQKLKARLR"
BASE COUNT         1145 a         1058 c         1149 g          997 t
ORIGIN      
        1 gcggggcggg ggacgccttt ttcggggaag acttcgagat cgatgaagaa cgcccgctgg
       61 agatcctgag ccgcctaatc ggccttcggg aggagcatga ggatcgtgtc gagggcttct
      121 tttaaaaaac tgtgttggct ttcgaaggtc gatagagagg atcttgacgt gagcgcgatg
      181 gttttgtgca agctcgcggt cagttcttcc gagacgttgt agctttcttg gagctgttga
      241 ttcatcgcct ccaactcctg gttaatggcg gagatgtcct cgttctgccg tcgaatcgtc
      301 gcaagccggt tagctaggtt atcggcaaac tcgttgaacg aaacgcccag ttgaccgacc
      361 ggaccgctag taggaccgaa atatcgagcg cctgattcgc cttggctgaa tcgggtagtc
      421 gtctcggaga gtatcgcgag gggtttaaag accgaacgat tgtagacata cccaaaaata
      481 aaggcggaaa acaagacaat cagaccaatc gcgatcacga aaacagcgac cgcgtgaaag
      541 ggcacgccgg tatttccgaa tgagatatcg aaccccagaa tccagccatt cgccaattta
      601 gcgaacgtga gcagccgcga gcgatctcgc catacgccag gagagagagt gggacctttg
      661 gacagagcct cgtccaaaac ggcgaggtcg accggttcca gatgctcaaa tagaactcct
      721 ttcgcgtaat cgggatgata caacacgacc cgctccgaat tgagcaagaa tccgaaggag
      781 tcttcagagt cagaaatacg tgcgactctc tcaatcaggt ggcgcaagcg aacgaatatc
      841 ccaactaccg ctatgggttg accgttgtgc aagaccggcg ccgaatagct gatcaagtgg
      901 tcttccagcg catccaaata gaacacttcc gaccaaccgg gcacctttct ggacaaaggc
      961 gtatagaacc agaccataga tgggttcgcc ttatcgtact cctctttggc taacgaaggc
     1021 tgtcgcctaa tcgaacggtc tgcgcttttt acgtaagtga tgtcaaaata cttttggtct
     1081 atttgcgggt tcaggtgaaa aaagcaggcg tccagctccg cgcagttggc cacaacggat
     1141 ttgagcaaag gagcgatttc tgtttctaaa cgatcgacga aagaagaggt cgtagggact
     1201 tcgcctggtt ctaataaagc cgagatttcc gataaaagcc attctatacg gctttcctga
     1261 ttgcggatga atttatcgaa ctctcccgct aactccaaac agtgtacggc ccttgcttct
     1321 gagagttctt ttttccgagt atcctgaaca tgatagaagc cccaaaaacc gacgcaaatt
     1381 gcgccaataa agaacagcag caaaaggacg cggtgggaaa cgaccaatcg ccactgtcct
     1441 tttcgagaag aagccttagc ggaacgtttt tgagaggact cttcactttt atgcgtcata
     1501 gtctatcctt caataactta aactcgagct ttcgggtttt cgccgctctt gctctcacgg
     1561 cgctagagta gcggaagcga catagaatac cgatagtgcc cgcgtttttt cagatttccg
     1621 tcacggctca attatatcag gattttgcgc agaatggagt acgatctgaa aagaatatca
     1681 attttttcgt cccgcgaatc ctgggagagt ggcgttcttt tcagtatacg aaaagaagag
     1741 ggttccgaag gaccctcttc tgtaagagcg tgagacatag atcgtctatg cgccctttta
     1801 aaacttgaaa cttggcgttg actatgcttt accgaagacg ggcttttagc ttctgaatct
     1861 cctcgagaga gagccctacc tggtttcgca gatacttcaa ggcgacgaac cggatgtttg
     1921 aatcgtccac cggctttccg tcgttcatct gggcgaaaat atcggcgatg atcttgcgaa
     1981 cggcagcata ggcgggagtt cccggtttca cgtgatagta gttgatgtac gatacgatgt
     2041 agtcgcgata gatatcttcg acttcgccgt tcatgatcgc ggccaaaaga gctgagacga
     2101 taccggctcg gtccttccct tccacacagt gaaccagata agggggttca ttctcgatca
     2161 taaagaggaa gcctaggcgc agtttcctgg cgaagtccgg gctgagcagg tccacgccca
     2221 tattgagatt gataatgtga ccggcttctc ccagagatcg atagaattcg gagtatttgt
     2281 aattctcagc cagttcctcg tccgagtcgg acaggttcac aacggtcttg atgccggcgc
     2341 cttccacaag cgcattggca tacggggatc ttggatcgtt aatagacgga tgcgaacagc
     2401 gatacagtgt gttgggcgcg atatctccca ttctcacagg tcggaaattg gcgaaaacga
     2461 cgtcggtgac gtagtcgctt cgaacatccg tgcgaacaag gttccgaatc tccagttcgc
     2521 ccaagtaggc gcctttttcg accaaagaaa tgttgacgcc catcccaagc gcgacggcgt
     2581 agacttggga aaagttgccg tagctgatcg ccatgaggag ttttccaccg gagatcctca
     2641 acaacggttg ccctctgtcg acatctccgt aattcctgac gaatgggaca acgagccggt
     2701 gatccccgaa ggccaactcg acgatatctc cgaactccag ccccgcttcg gtggccgagt
     2761 cctgtaaaag atcggtatac gtgttaccgt acttatcgac ctcggccact ttcccgctga
     2821 gtcctgcgaa gatactcacc gtgaagatcg cgaagatcaa gacaaaaagg atacgtttca
     2881 cacacaacac ctccataaaa gggttttttt ggcagcgttt ggctacgcgc tcactatgat
     2941 tctaacataa aaacccggaa ggccggatat gtgatttttg aaatctttca aacggggttt
     3001 caaagatcgt atggcgtgtg tgtcaagtta tggggtgaaa tcaagaaaaa ggctcttcga
     3061 tacattactt gaagtccact gatcatccaa gaaatacaaa gtaacataag aggaataaaa
     3121 agagactgcg agatggccga ttatgcgatt agagcggttt agcgaaacgg tctaagggtc
     3181 tacagaatta ttacgataga agacgttaat taagtacgtc ttgtaaattg tcgaagagcc
     3241 aagaaaaaag gccagaatcg agcgcgattt ttgtcgttat agtcatcggg gcgtaagcga
     3301 cccttctttt cgtgaggccg acttccagtc ggcctgcctc tctgggaacg ccaacttctg
     3361 ttcggcgtgt cgatggaaac gccaactttc aagttggcat gtcttaaaaa aacagggttc
     3421 acacggaagg catagagaac gcagagaaaa aaactctggg aacgcgctgg gaacaccagg
     3481 gaacgccaaa ctttagtttg gcatgtcttt tgtttttctt ggaacgttcc caagcgcggc
     3541 atcgcggcgt aagcgaccct tcttttcgcg acggcgcgat accccgacgt catcgcgggg
     3601 taaaaaaaca aaaggcgggg atcgcgagac ttcgtgggct tccgaagtca gcccgctggt
     3661 taccccgcga taaccccgca cgagcatcgc ggcgtaggcg cccatttctt ttcgcgacgg
     3721 cgcaataccc tcgctttcgt tcgttctaaa gattaagaac aggtttcgca cggagggtac
     3781 agagaacaca gaggataaac tgacaaggga cagggttctc ccggaagagg aaaagatatg
     3841 ccggactaaa gtccggcttt cccagggcgg cgtaaaaatt gcagaaggcg aatcgcgagg
     3901 ctttgtgggc ttccgaaatc ggccccgttg gttaccccgc gataacccca cacgggcatc
     3961 gcggcgtaag cgccctttct tttcgcgacg gcgcgatacc ctgacgtcat cgcggggtaa
     4021 aatgcataag gcggggatcg cgagacttcg tgagtttccg aaatcggccc cgttggttac
     4081 cccgcgatcc ccgcacgagc atcgcggcgt aagcgtactt tatagcaatc ctcgcgttgt
     4141 tcaatcgatt cacatactat ttacgaaaaa gaaggagggg aaaagattga caaaggcgga
     4201 cagatatgat aatatacccg aggtttccaa gagaaaggta ttaagggaat agggaaagtg
     4261 cagggaccgg tggaaagaaa ggtcaccggg aaattttcgg tctttgaaaa gcagataata
     4321 gacgaataag caaccagacg cgaagtaag
//
LOCUS       DAOPXN010000191         4320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20499_length_4320_cov_5.435873, whole genome shotgun sequence.
ACCESSION   DAOPXN010000191 DAOPXN010000000
VERSION     DAOPXN010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20499_length_4320_cov_5.435873"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(294..1931)
                     /gene="hcp"
                     /locus_tag="PKM49_08880"
                     /gene_synonym="priS"
     CDS             complement(294..1931)
                     /gene="hcp"
                     /locus_tag="PKM49_08880"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNY82946.1"
                     /translation="MFCFQCQEAAKNIGCTVRGVCGKTEDVSNLQDLLIFALKGLSVY
                     ALKAREAGVSAKEADRRVIEGLFATITNANFDRRYFVDAIRGAIAERESLKKALGGSM
                     ATLSDDERDAATWTFREEAQLDKKAADVGFLVERDEDIRSLKALILFGLKGMAAYTEH
                     AEVLGFSDPKIHTFIQKALAATLSTRLSAEDLVALTLETGEYGVAAMSLLDRANTTTY
                     GHPQITKVNIGVRKNPGILISGHDLKDLEELLEQTQGTGVDVYTHGEMLPAQYYPAFK
                     KYEHFAGNYGNAWWKQDKEFESFNGPILMTTNCLVPPKDSYKDRVTVTGVVGFPGLKS
                     IPAREPGKKKDFSALIAQAKTCKPPKEIETGEIVGGFAHETVFALADQVIAAIKEEHI
                     KKFVVMAGCDGRMKSREYYTEFAEKLPKDTVILTAGCAKYRYNKLPLGTIGQIPRVLD
                     AGQCNDSYSLALIALKLKEAFGFESINQLPIVYNIAWYEQKAVIVLLALLYLGVKNIH
                     LGPTLPAFLSPGVTNVLVEKFGISGITDADADLKAFL"
     gene            complement(1941..2297)
                     /locus_tag="PKM49_08885"
     CDS             complement(1941..2297)
                     /locus_tag="PKM49_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNY82947.1"
                     /translation="MIERVFPVTQSDKKTIEVVVDDDHAMINHIVLAKGDAVPEHDSN
                     SNVYLIVARGEMTIALGPQEAQVYPAGRILAVPYQTRMRISNAGEQALEFFVVKAPNP
                     KVLLKESGKAKKSGGA"
     gene            2809..3501
                     /locus_tag="PKM49_08890"
     CDS             2809..3501
                     /locus_tag="PKM49_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNY82948.1"
                     /translation="MMFEELTPILLKASLFVGLTPKELNALLSCVSASTAVYKKNDVI
                     AHGGDLFQGVGMIIRGEAVVTKENALGERLMMTHLKSGDLFGEMLPFAGDGHYIATVE
                     AKTACSALFIPKDKVFPSCFSLCHSHRVMLQNYLKIVSKKSLLLNQKIEYLRIRSLRG
                     KIAAFLLDMYEQTGSRTLELPFNRNEMAEFLNVSRPSMSREMGRMKHDRVIDFRKNTF
                     LILDPARLKAFI"
     gene            3560..>4320
                     /locus_tag="PKM49_08895"
     CDS             3560..>4320
                     /locus_tag="PKM49_08895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNY82949.1"
                     /translation="MQIRRLEKRSHIDQVIRCLEQAFQRSFSEPRDRLWLDDESNWQW
                     IWGLEEERRIVGTYVSYEVQVCLRGKAFRGHYLDALTTLPNHRNRGWIKEMMLRDFQD
                     CRENNIPVILLDPFKHAYYRKYGFDTIADCKSLTVPWSLLSDIVPKSEGEDGYSVHFA
                     KLSVDAYAQMAYAETRRRAFENAYYTEMIRPAPYENAVFLDRDLWIAIALDKEKRAQG
                     YLLFTYENRVLRVQNQRFFTLRAFYALKKLILQHR"
BASE COUNT          895 a         1208 c         1192 g         1025 t
ORIGIN      
        1 tttgagaaag tggcttttat cgccgtttgt agcactttta gtcgaaagtt gggctaggaa
       61 cgccgaactt tataggttat cgcggggtaa ctaacaggac cccgcgatcc gctccatgtc
      121 tttctaaaaa cggtttcgcg tcgtcgcaaa aagaagaagc gcttacgccg cgatgccccg
      181 cctgggaacg cttcccacct ccgtggcctc tgtgccctct gcgcgaaccc atttttttca
      241 aaacgtgcca acttaaaagt tggcattccc agggcgctcc taggcgcttc cgtttacagg
      301 aaggctttca ggtccgcgtc ggcgtcggtg attccgctga tgccgaactt ttccacgagc
      361 acgttggtca ccccgggtga gaggaacgcg ggcagagtcg ggccgaggtg tatattcttc
      421 accccgagat agaggagcgc taacaggacg atcacggcct tttgctcgta ccaggcgatg
      481 ttgtacacga tcgggagctg gttgatgctc tcaaacccga aggcctcctt cagtttgagt
      541 gcgatcagcg ccaaggagta cgagtcgttg cactgtccgg cgtcgagcac cctcggaatc
      601 tggccgatcg tgcccaacgg gagcttgttg taccggtact tcgcgcaccc ggcggtgagg
      661 atgaccgtgt ctttcgggag cttttccgcg aactccgtgt aatactctcg gctcttcatc
      721 cgcccgtcac aaccggccat cacgacgaat ttcttgatgt gttcctcctt gatcgcggcg
      781 atcacttgat ccgcgagggc gaacacggtt tcgtgggcga atccgccaac gatctctccc
      841 gtctcgatct ccttcggagg tttacaggtt ttcgcttggg ctatgagcgc agaaaagtcc
      901 tttttcttgc ccggctctcg tgcggggatg ctcttcagcc caggaaagcc cacgacaccc
      961 gtgacggtga cacggtcctt gtaactgtcc tttgggggca ccaagcagtt ggtcgtcatc
     1021 aaaatcggcc cgttgaagct ctcaaattcc ttatcttgct tccaccaagc gttgccgtag
     1081 ttgccggcga aatgctcata ctttttaaaa gctgggtagt attgggccgg caacatctct
     1141 ccatgggtgt acacgtccac gccggtcccc tgagtttgct ccaacagctc ctcaaggtct
     1201 ttcagatcgt ggccgctgat caggatcccc gggttttttc gcaccccaat gttcactttc
     1261 gtgatctggg gatgcccgta ggtggtcgtg ttcgccctgt cgagcaggct catcgccgcg
     1321 acaccgtact cgcccgtttc cagcgtgagg gcaaccaagt cctcggcgga aagccgggtg
     1381 ctgagcgtgg ctgccaaagc tttctggatg aaggtgtgga ttttgggatc cgaaaaaccc
     1441 aacacctcgg cgtgttcggt gtaggcggcc atccccttca atccgaagag gatcaacgcc
     1501 ttgagcgacc ggatatcctc gtcgcgctcc actaggaacc cgacgtccgc ggcttttttg
     1561 tcgagctggg cttcttcgcg gaaggtccac gtcgccgcat ctcgctcgtc gtcggagagg
     1621 gtcgccatag aaccaccgag cgcctttttc agagactccc gttccgcaat cgcgcccctg
     1681 atggcatcta cgaaataacg gcggtcgaag ttcgcgttcg taatcgtcgc gaaaagccct
     1741 tcgatgacgc gacgatcggc ttctttcgcg ctcacccccg cttccctcgc tttgagggca
     1801 tagacggaca ggcctttgag cgcaaagatg agcaaatcct gcaggttaga gacatcttcg
     1861 gttttgccac atacgccacg gacggtgcat ccgatgttct tcgccgcctc ttgacactga
     1921 aaacagaaca tacgggacat tcacgcgcct cccgactttt tcgcttttcc gctttctttg
     1981 agcagcacct tggggttcgg ggctttcacc acgaaaaact ccagagcctg ttcgccggcg
     2041 ttcgagattc tcatccgcgt ttgataaggc accgcgagga tgcggccggc gggatagact
     2101 tgggcttcct gcggtcccaa ggctatggtc atctctcccc tcgcgacgat caggtagacg
     2161 ttcgaatttg aatcgtgttc ggggacggca tcccccttcg cgaggacgat gtggttgatc
     2221 atcgcgtggt cgtcgtcaac gacgacctcg atcgtctttt tgtcggactg cgtaacggga
     2281 aatactcttt ctatcataaa accacctcct gtgggaacgc cgaacttcag ttcggcatgt
     2341 tttttctttc gctttcctgg gaacgctctg agagtgcctt gggaacttcg acttttagtt
     2401 cggtatgtct tttctttcgc ctttctggga acgctctggg agcgccttgg gaacgccaac
     2461 ttttaagttg gcgtgtcttg gaaaacaggg ttcgcacaga gaccacagag gcacagagga
     2521 gggattactg agagcgcctg gtggcgttga cttcctagtc ggcttacctt tggtcttcgt
     2581 agtaaggtaa acttgccgga ctaaagttcg gcgttcccag ggcgtttctc tgtgttctct
     2641 ctgtgtactc tgtgccctct gtgcgacaca tcttttagtt ttggagaaac atgccgaact
     2701 aaagttcggc gttctcaagg tgctctcagg cgaaatagtt tcttttccgc ccgaattata
     2761 ttataatcga aggcgaaaaa acagtatccc aggatacgtg atggaggaat gatgttcgaa
     2821 gagttgaccc cgatactcct caaagcctca ctctttgtcg ggctcacgcc gaaagagctg
     2881 aacgccctac ttagttgcgt ttccgcgtct acagccgtgt ataaaaagaa cgacgtgatc
     2941 gctcacggcg gggacctttt ccaaggggtg gggatgatca tccgagggga agcggtcgtg
     3001 accaaggaga acgcgctcgg agagcgtctg atgatgacac acctgaaaag cggggacttg
     3061 ttcggggaga tgctgccctt cgccggcgat gggcactata tcgccaccgt cgaagcgaag
     3121 accgcatgct cggccctttt tatccccaaa gacaaggtct ttccgagttg cttcagcctg
     3181 tgtcacagcc accgtgtgat gcttcaaaat tacctgaaga tcgtctcgaa aaagtcgctg
     3241 ctgctcaatc agaagatcga gtacctgcgt atccgaagcc tacgcgggaa gatcgccgcc
     3301 ttcttactcg atatgtatga acaaaccggg agccgcacat tggagctgcc ttttaaccgg
     3361 aatgagatgg ccgagttcct caacgtctcg cgcccgtcga tgtctcgtga aatggggcgg
     3421 atgaaacacg acagggtcat cgacttccgg aagaacacct ttctgatttt agacccagcc
     3481 aggttgaagg ccttcatatg aaggatggcc gagtcggagg gcgggtattc tccgcgagtg
     3541 gagaaaggga ggaaacgtga tgcagattcg ccgtcttgaa aagcgctcgc acatcgacca
     3601 agtgatccgg tgtttggaac aggcctttca acgctcattt tccgagcctc gcgaccggtt
     3661 gtggttggat gacgagagca actggcaatg gatctggggg ttggaggaag agaggcggat
     3721 cgtcggcacc tacgtcagct acgaggttca ggtatgcctg cgggggaaag cctttcgggg
     3781 acattacctg gacgccctga cgaccctccc gaaccatcgc aatcgcggat ggatcaaaga
     3841 gatgatgttg cgcgactttc aagactgccg cgaaaacaac attccggtca tcctactgga
     3901 ccccttcaag cacgcctact accggaaata tggcttcgac acgatcgccg actgcaaaag
     3961 tctgacggta ccctggagtc tgctctcgga catcgtgccg aaaagcgagg gcgaggacgg
     4021 ctactcggtc cacttcgcca agctgagcgt cgacgcgtac gcgcaaatgg cttacgccga
     4081 aacacgccgt cgcgcctttg agaacgccta ttacacggag atgattcgtc cggcgcccta
     4141 tgaaaacgcc gtgtttttag atcgggacct ctggatcgcg atcgccttag acaaggaaaa
     4201 acgggcccaa gggtacttgt tgttcaccta cgagaatcgg gtgctacgcg tccagaacca
     4261 gcgttttttc acactgcgcg ccttctacgc gctgaagaag ctgattctcc agcaccgaga
//
LOCUS       DAOPXN010000192         4316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20527_length_4316_cov_5.098334, whole genome shotgun sequence.
ACCESSION   DAOPXN010000192 DAOPXN010000000
VERSION     DAOPXN010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20527_length_4316_cov_5.098334"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PKM49_08900"
     CDS             <1..1317
                     /locus_tag="PKM49_08900"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNY82950.1"
                     /translation="ISFTFGLVLYLLFQKGSWNKEITYQSGAVIVVSIWTLIIFFSAL
                     TFLFARLCNFTQAMFESASGWTTTGLTIMDVANTPQIFLFWRSIIQFVGGAGFAVIML
                     SSIAGGVSAGVYRAEGRSDNLLPNIKKTAKSILIIYCSYALFGFVALIATGTHPADAF
                     HHTLTGLATGGFSNRAGNVGEIDRLSTEIILIALMLLGATSFGIHFLIWQRSFHTIRK
                     NVEPKLFFFLLALFSVLIVVAITGIVYPNLPETIRHSVFQAVSAISGTGFQTTNFSGW
                     PQSALFLLILLMCCGGMMDSTAGGVKQLRLYILFKAVAIEIVHFCLPVGSIRKYTIWK
                     GNTRIDLDERLIKNTAVFFTLFGAHFLIGVSIFLFLGYDLTTACFELASALNNVGMSL
                     GATNANASGVFLWNQIFAMFFGRLEFAVILFAISKVVRDGIQVVRA"
     gene            1321..2253
                     /locus_tag="PKM49_08905"
     CDS             1321..2253
                     /locus_tag="PKM49_08905"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNY82951.1"
                     /translation="MIKKIMVHEKNWYGRLDKFLRKELDEIKLTAIYSLIRKGDVKVN
                     ERRVKKPDYPVQIGDTVEVYLPDKEAGKERITRDLSEKRKLIPRKLALPRVYEDEAMI
                     VLDKPSGLSIHPGEGAKQKTTVIEGLLFESKERGYEPYLVHRLDKDTSGLLVIAKSIE
                     VARILGETFRERNAQKRYAAGVSGAINRELHIDTPLDSKEALTHLKPLVVYGREGRFL
                     TLLEIEILTGRKHQIRRHLSLHHLPVLGDALYADAELNHWVRARGLKRLFLQSSYLQF
                     PHPLTGKTVHFTLPLSSDLQAFLDACEPIERPSR"
     gene            complement(2921..3340)
                     /locus_tag="PKM49_08910"
     CDS             complement(2921..3340)
                     /locus_tag="PKM49_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82952.1"
                     /translation="MAKMIPLNFPLTVEQVEKLSKVVAITTASRSRHTDNERITKASF
                     VRVLIDLAPLEEIEYSEIRSEEELKERLSATFNAPSDPSAHRIKPADLAEVYHKFAGD
                     DHRKWVAGDFINTLFERGWIEARSSEEMWAIILKATR"
BASE COUNT         1030 a         1101 c         1079 g         1106 t
ORIGIN      
        1 atctctttta ccttcggttt ggtcttgtac ctgctctttc aaaagggtag ttggaacaaa
       61 gaaatcacct atcaatccgg cgcggtcatc gtggtgtcga tatggacgct catcatcttt
      121 ttttctgctc tgaccttcct cttcgcgcgc ttgtgcaatt ttactcaagc gatgtttgaa
      181 agcgcaagcg gttggacgac gaccgggctt acgattatgg acgtcgcgaa cacccctcaa
      241 atatttctgt tttggaggtc catcatccag ttcgtcggag gcgcgggttt cgccgtgatc
      301 atgctctctt ccatcgcggg cggtgtcagc gccggagtct atcgcgcgga aggaagatcg
      361 gacaacctct tgccgaacat caagaaaacg gcgaaatcta tactcatcat ctattgctcc
      421 tacgccctct tcgggtttgt cgcgttgatc gccacgggga cgcatccggc agacgccttc
      481 caccacacgt tgacgggttt ggccaccggg ggcttctcta accgcgcggg gaatgtcggg
      541 gagatcgatc gcctttccac cgagatcatc ctgatcgcgc tcatgctgtt gggagcgacg
      601 agtttcggta tccatttcct catttggcag cgtagcttcc acacgatccg aaagaacgtg
      661 gagccaaaac tctttttctt tttactcgca cttttctcgg tattgatcgt tgtggcgata
      721 accgggatcg tctacccgaa ccttccggaa accatcaggc actccgtatt tcaggcggtt
      781 tccgcgatca gcgggacggg attccaaacg accaatttct ccggttggcc tcaaagcgcc
      841 ctctttttac tcatcctcct gatgtgctgt ggggggatga tggactccac cgccggcggc
      901 gtgaaacaat tgcgtctcta tatcttattt aaagcggtgg ccatagaaat tgtccatttc
      961 tgtctgcccg tcggctcaat ccgtaagtac acgatctgga agggaaacac cagaatcgat
     1021 ttggacgaac ggctgattaa aaacaccgcc gtttttttca ccctctttgg cgcgcacttc
     1081 ctcatcgggg tttctatctt tctgtttctt gggtatgacc tgaccaccgc atgcttcgaa
     1141 ctcgcctccg cgctcaacaa cgtggggatg tctctgggag cgacgaatgc gaacgcttcc
     1201 ggggtgttct tgtggaatca gatcttcgcg atgttcttcg gccgtcttga gttcgccgtg
     1261 atcctttttg cgatctcaaa ggtcgtgaga gatggcatac aggttgttcg ggcctaaacg
     1321 atgataaaga agataatggt acacgaaaag aactggtacg gccgcctcga caaattcttg
     1381 cggaaagagt tggatgagat caagctgacc gcgatttact cgctcatccg caaaggggac
     1441 gttaaggtca acgaacggcg cgtgaaaaag cccgattatc ctgttcaaat cggagatacg
     1501 gtcgaagtct accttcccga caaagaagca ggaaaggaga gaatcacgcg cgacttaagc
     1561 gagaaacgga agttgattcc acggaagctc gccctgccgc gtgtttacga ggatgaggca
     1621 atgatcgttc tcgataagcc atcgggcctc tccatccacc cgggagaagg ggcgaagcaa
     1681 aaaaccaccg tcatcgaggg gcttcttttc gagtcgaaag agaggggtta cgaaccctac
     1741 ctggtccacc gactcgataa ggacacctcg ggcttgctgg tcatcgcgaa atcgatcgag
     1801 gtcgcgagga ttttgggaga aacctttcga gaacgaaacg cacaaaaacg ctacgcagcc
     1861 ggcgtttcgg gggccatcaa tcgagaattg cacatcgaca ctcccttgga ttcaaaagag
     1921 gcgcttaccc atctcaaacc ccttgtggtg tatggacgag aagggcggtt cttgaccctt
     1981 ttagagatag agattcttac cggaaggaag caccagatca ggcgacatct gtcgctccat
     2041 catcttccgg ttttgggcga cgcgctctac gccgatgccg agctcaacca ttgggttcgg
     2101 gcgcgcgggc tgaagcgatt gttcctgcaa agtagctatc tgcaattccc tcacccactg
     2161 acaggaaaga ccgtccattt tacgctaccc ctgagttccg atctccaagc gtttctagac
     2221 gcttgcgagc ccatcgaacg accctcacga tagagaccca cgactcctcc ttcttcgtct
     2281 caggctatta ggcgtctacc ggtgtcgcgt ctcgacgcga tttctcggcc cacctccgtc
     2341 agatatatca atatttatga ttttatattt tcttatactc gtcgatttat atatatcact
     2401 tttttataaa tgcttatttt tctatttttg tatatacgct gattgtttaa tcgcatttat
     2461 tctgctatta gatatataag aatttatata ttatgaatta gtcggaggtc acccgcgttt
     2521 aactcacaga ccgtctctct ttcgtgattt tattcgcaca gaccttatgg gaggcgcggg
     2581 gatagcgcgc cggcacgtat ttggcaagac caacgccgag gaaagtcccc aatgccgcgg
     2641 gtaattctcc ctgaggtacg ggttatcggg tttcgaataa aaaaagccga ctgagaagtc
     2701 ggcctctggg acgggcttta agcgactcta aaagaggttg cgccttattc ctacgatatt
     2761 gcctgagacg ccaatacggg tcatagcagg cgcacacagg aggaataggg cgagttcgtg
     2821 gggtaaaatt cgggcgttag gggtttatcc acccatttgg gatgggtggg gcgcattctg
     2881 gggccgattt tgacgcaaca aaaggataca ttttgttgcg tcaacgggtg gctttcaaga
     2941 taatcgccca catttcttca gaagagcgcg cttctatcca cccgcgctcg aaaagcgtgt
     3001 tgataaagtc cccggctacc cacttccggt ggtcgtcacc ggcgaatttg tggtacacct
     3061 cggcaagatc cgccggttta atgcgatggg cgctcggatc ggaaggcgca ttaaaagtcg
     3121 cggacaggcg ctctttcaat tcctcttccg agcggatctc cgaatattct atctcttcca
     3181 aaggggctaa atcaataaga acgcgcacga agctcgcttt cgtaatgcgc tcgttgtctg
     3241 tatgccggct cctgctcgcc gtggtgatcg ccaccacctt cgataacttt tctacctgtt
     3301 ctaccgtcaa aggaaaattt agaggaatca tctttgccat agtcgcctcc aattataaga
     3361 atataaaggc attatatcat ataaatagat aaaaaacaaa taaggcgata tcgaaaatag
     3421 tgaaaatata gtagactgaa aatagaataa aacgatagat ataattagcg aatactataa
     3481 atagcgataa ttataaatgc gctctgcgga gaatgcgatt ctcttcttat atgcaataaa
     3541 tcaaaggctg atgaatacga cgatcgaccc tcttaagtag gaacagaaag cgtttcacac
     3601 caccaggaat gcgtgtgtga tacttctttt cttgaagaag tataagatat aggaatgagg
     3661 atgaccgtgt gtgttatcgc gggtaagcgg gttttgcgca cccgggctct gccctttgcc
     3721 ttttgttagt gtcgaggaaa aatatgccgg actaaagtcc ggcgttccca agggtatcgc
     3781 ggcgtcgcgg acggaaatag aggtaacgcc gcgatgcctg cgcacgggaa ccttaccagt
     3841 gataagattt aaccatgcca aactaaagtt tggcgttccc aaggcgttcc cggggcgttc
     3901 ccagggcgtt cactaaaatc ttttctccgt gccctccgtg tgaccgccct tttttcgagc
     3961 cgttgcggac ggaaatagag cttacgccgc gatgcctgcg cacgggaacc tttccagcga
     4021 aaagattaaa ccatgccaaa ctaaagtttg gcgttccaag ggcgttccga aggcgtttcc
     4081 agggcgttca ttaaaatctt ttccccgtgc cctccgtgtg aacgcccttt tttcgagcag
     4141 tcgcggaagg aaatagagct tacgccgcga tgcctgcgca cgggaacctt tccagtgaaa
     4201 agattaaacc atgccaaact aaagtttggc gttccaaggg cgttcccaag gcgtttccaa
     4261 tgcgttcatt aaaatctttt ccccgtgccc tccgtgtgaa cgcccttttt tcgagc
//
LOCUS       DAOPXN010000193         4308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20572_length_4308_cov_5.284270, whole genome shotgun sequence.
ACCESSION   DAOPXN010000193 DAOPXN010000000
VERSION     DAOPXN010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20572_length_4308_cov_5.284270"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKM49_08915"
     CDS             <1..201
                     /locus_tag="PKM49_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82953.1"
                     /translation="PDRLSGVDIPLLSRILALADTYDSMTSDRAYRTGLAPGDAKHIM
                     ARLSGKQFDPSLLDTFLNLPPF"
     gene            249..2003
                     /locus_tag="PKM49_08920"
     CDS             249..2003
                     /locus_tag="PKM49_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HNY82954.1"
                     /translation="MIDVTLKIALTVRNNPGKFALLIGSGLSQSAGIPTGVEVVRSIL
                     SELARASGVDTPDPTEWYRLHFGEDPNYSRLLEKISQTRTERKGVLQKYYEATPEERA
                     KGVKVPTAAHRAIASLVLKGYFRMIVTTNFDRLLEKALEEEGVIADVVSTEDAIWSGA
                     LNYIHSACTIWKINGDYKDARIRNLSGELEAYPEIYDKYFERLFEDFGLIICGWSANY
                     DVALRKALIRFSKKRYGLYFCQLTPATQNENAASLVSLLGGEFVPIKSGDAFFQELKD
                     KVVALEEGVSREPFSERVAVRLVQEYATNSQKLSMLNLLFQEEMQALYSFLSTLLPSE
                     PTRTCLRKTENACRKLTKMLGYLVFAASSAERDLLIRKTFRTFLGSKELETHHAEERL
                     RALLDYPTLLFAYGVGIMALEKDRYAFLFELLHLTASAQKGELEPLFCRCHPDNVYGR
                     ITAPGSSTGDSVDAHKGPFAWNYYLRDLLWDFFQEHFVNRTYYERIFNLFEFFVSLLW
                     TSREFDRTTDEAEKAPLGVFAVEVNTDPRQMAMISEFVSRMRETEKSPIALHSRFWTE
                     WRHALIDPMEHLLRRLEF"
     gene            complement(2056..3228)
                     /locus_tag="PKM49_08925"
     CDS             complement(2056..3228)
                     /locus_tag="PKM49_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HNY82955.1"
                     /translation="MSQYMYTDASNHSLEGIEEIVINAIEADITLANASDNQVSWEAD
                     WRDAVGHPEIIREGNRLIIRFNERNIYFNFFGISIGKTRERAWKGSLEAAKIYIPNGF
                     PRLFVNLVSGDVLFDNLNLERSCRFNTLSGNVRGAHLHAKEVKVTTKSGDISLKGIIC
                     ENLILETFSGDVNMISHSCRRAHLKSKSGDIRISEGLGDLKELTVDNISGDIFVDRAP
                     AMSKLTSISGDIRFSSREKKGIDWSLRSVSGDIVLETADIRSRLFFSTISGEMRFLNV
                     KPGYSAKNEYVLGTGEDGKIALQTVSGDARLQFMETDPSQSVKEEPIASAEGDSERKA
                     YTKRFVQPDENVQKVVQMNSQGILSKEEAVDLLHSMGYKEEEIALFLEKEEQQEGE"
     gene            complement(3262..3468)
                     /locus_tag="PKM49_08930"
     CDS             complement(3262..3468)
                     /locus_tag="PKM49_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82956.1"
                     /translation="MIVLSILGGIFLVFLAFKFLSVILGVLISVGVLVLVGLIFAGIL
                     TILGPLLILILVIAGGIWLLKTIF"
     gene            complement(3465..3857)
                     /locus_tag="PKM49_08935"
     CDS             complement(3465..3857)
                     /locus_tag="PKM49_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HNY82957.1"
                     /translation="MRKTFTTCPACSGALHITEYTCGKCGTVIRGKFELDEFLRLSED
                     QLFFLKVFIKHKGNLSEIQKEFEISYPTARNRLEEIIRRMGFELQENPEKTESQRILE
                     MLEKKEITSKQAMEMLRHLNEKEGGVSS"
BASE COUNT         1007 a         1144 c         1069 g         1088 t
ORIGIN      
        1 cccgatcggc tctcgggggt tgacattccc ctgctgtcta gaattctcgc tttagcggat
       61 acctacgatt cgatgaccag cgatagagcc tatcgcaccg gcctcgctcc cggagacgcg
      121 aaacacatta tggcgcggct aagtggaaag caattcgatc cttccctttt ggacaccttc
      181 ctcaatttgc cgccgttttg agttcacaag ccctcttctg gagcgttctg ctccgaaagg
      241 agaccattat gatagatgtg accttgaaaa tcgccctgac cgttcgcaat aatccgggga
      301 aattcgccct gttaatcggc tcgggattgt ctcaaagcgc ggggattccg accggtgtcg
      361 aggtcgtgcg ctcgatcctg tccgaactcg ctagggcgag cggagtcgat acccccgatc
      421 ctacggaatg gtaccgcctt cattttggag aggaccctaa ttattcgcgg ctgctcgaaa
      481 aaatctctca aacccggaca gaaagaaagg gggttttgca aaaatactac gaagctaccc
      541 ccgaggagcg ggcgaaaggg gtgaaggtgc ccaccgccgc ccatcgggcg atcgcctctc
      601 tcgtgttgaa ggggtacttt cgaatgatcg tcaccaccaa cttcgatcgc ctgctagaaa
      661 aggctttgga agaagagggg gtgatagcgg atgtggtttc taccgaggat gccatatgga
      721 gcggcgcgct taattacatt cattccgctt gcacgatctg gaagatcaac ggagactaca
      781 aagacgcccg gatacggaac ctctctggag aactggaggc ctatcccgaa atctatgaca
      841 aatactttga acggctattc gaggacttcg gattgatcat atgcggttgg tccgccaact
      901 acgacgtcgc cctcagaaaa gcccttatcc ggttttcaaa aaagcgctac ggcttatatt
      961 tttgtcaact caccccggcc actcaaaacg aaaacgccgc ttctctggtc agcctgttgg
     1021 gaggcgagtt cgttccgatc aaatcagggg acgccttttt tcaagagttg aaggacaaag
     1081 tcgtcgcttt ggaggaaggg gtgtcgcggg aacccttttc ggaaagggtg gccgttaggc
     1141 ttgtacaaga gtacgcgacc aactcgcaga agctttcaat gttgaacctg ctgtttcaag
     1201 aagagatgca ggcgctctac tcttttttgt cgacgttgct gccctctgaa ccgacacgga
     1261 cgtgtttgcg caaaactgaa aacgcctgcc gtaaactgac aaaaatgctc gggtatctgg
     1321 ttttcgccgc ttctagcgcg gagagagacc tgctcatccg caagaccttt cgcactttcc
     1381 tgggttcgaa agagctcgaa acacatcacg ccgaagaacg ccttcgcgcc ctactagact
     1441 atccgacgct gttgttcgcc tatggcgtcg ggatcatggc tttggaaaag gatcgatacg
     1501 cttttctgtt cgagcttctc cacttaaccg cctctgcaca aaagggggag ttggaacccc
     1561 ttttctgtcg ctgccatccg gataacgtgt acggccgaat tacggcgcct ggctcctcaa
     1621 cgggagactc cgtcgacgcg cacaaggggc cattcgcttg gaactactat ttgcgggatt
     1681 tgttatggga ttttttccag gagcatttcg tgaacaggac ctattacgaa aggatattca
     1741 acttgttcga gttcttcgtc agccttttgt ggacatcacg cgaattcgat cggaccacag
     1801 acgaagcgga aaaggcgccg ttgggggtct tcgccgtgga agtgaatacc gatccccggc
     1861 agatggcgat gatctccgaa ttcgtctccc gcatgagaga gacggaaaag tccccgatcg
     1921 ccttacacag ccgcttctgg acggagtggc gccacgcttt gatcgatccg atggaacacc
     1981 tgttgcgccg cttagaattc tgaacagaaa aaaaacggga ttcgatgggg gagcgcctcg
     2041 ctaagaacga ggcgctcact ccccttcctg ttgttcctct ttttccagaa agagggcgat
     2101 ctcctcctct ttgtacccca tcgagtgcag taaatccacg gcctcctctt tggaaaggat
     2161 gccctgacta ttcatttgga cgaccttctg gacgttctca tccggctgta cgaatcgctt
     2221 ggtgtaggct ttacgctcag agtctccttc cgcactggca attggctcct ctttgacgga
     2281 ttgcgagggg tcagtttcca taaactgcaa tcgcgcgtct ccggacaccg tttgtaatgc
     2341 gatcttccca tcctcgcccg tccccaacac atattcgttc ttcgcgctgt atccgggctt
     2401 cacgttcaga aaacgcattt ccccggatat tgtagaaaag aacaggcgag aacggatgtc
     2461 ggccgtttct aaaacgatgt ccccgctcac acttcttaag ctccaatcga tccccttttt
     2521 ctctcgggaa gaaaaccgaa tatctccgct gatcgacgtt aatttcgaca tagcgggagc
     2581 ccgatcgacg aaaatgtcgc ccgaaatgtt gtccacggtc aattccttga ggtcaccgag
     2641 gccctcggaa atgcgaatgt ccccggattt gcttttcaaa tgagcccgcc ggcaagaatg
     2701 gcttatcatg ttgacgtcgc cgctaaaggt ctctaggatg aggttttcgc agatgatccc
     2761 cttcagactg atatctccgc tttttgtggt tactttcacc tctttcgcgt gtaaatgggc
     2821 ccctcggaca ttcccgctga gggtgttgaa acggcaggag cgttccagat tcaagttatc
     2881 gaacaagaca tcgccggata caaggttcac aaataggcgc gggaacccat tcggaatgta
     2941 tatcttggct gcttctaaag agcccttcca tgctcgctct ctcgtttttc ctatagagat
     3001 gccgaaaaaa ttgaagtaga tatttcgctc gttgaagcga atgatcaatc ggttgccctc
     3061 tcgaatgatt tcggggtggc cgaccgcgtc ccgccagtcc gcctcccaac tgacctgatt
     3121 gtcagacgcg ttcgccaggg tgatatctgc ttctatcgcg ttgatcacga tctcttctat
     3181 tccttctaac gagtgattcg aggcgtccgt atacatatat tgagacatag tccttcctcc
     3241 tttcggttgc tcgaggttaa gctaaaatat cgtttttaaa agccaaatgc cgccggcgat
     3301 cacgagaatg aggatgagaa ggggaccaag aatggtgagg ataccggcaa atatcagtcc
     3361 caccaagact agcactccga ccgaaatcag gacgcctaag atgacggaga ggaatttaaa
     3421 agccaaaaac acgagaaata tccctccgag tatcgagaga acgatcacga cgaaacgcct
     3481 ccctctttct cgttcaggtg tcgaagcatc tccatcgctt gtttcgacgt aatctctttc
     3541 ttctccagca tttcgagtat cctctgcgat tcggtcttct ccgggttctc ctggagctca
     3601 aaccccattc ttctgataat ctcttccaat ctgttccgcg ccgtcgggta ggagatttcg
     3661 aattctttct ggatctccga aagattgcct ttgtgtttga taaagacttt taggaaaaac
     3721 aactgatcct cggacaatcg aaggaactcg tccagttcga atttaccgcg tataacggta
     3781 ccgcatttgc cgcacgtgta ctcggtgata tgcaaggccc cgctacaggc cgggcaagtc
     3841 gtgaatgttt tccgcatttc cctacctcct cgcttcatat ttcttctatt atagcacaac
     3901 gaatttgaag agtcaagcgg agaaatgaat aatctcaaag tccgatttaa taatattgtt
     3961 ttttcggttc tttgtgtgtg tcaagttatg gggtgtactc aagaaacaag gccagaattg
     4021 atcgcacgat ttttagtcta tgtagtcatc gcggcgtaag cgctttttct tttcgcgacg
     4081 gcgcgatact ttttcttatg cgtgctgggc gcggcgtaaa aaagacaaag gacggatcgc
     4141 ggggtcgtaa aaaccactta ccccgcgata accgcacacg agcatcgcgg cgtaagcgct
     4201 ttttcttttc gcgacggcgc gatactttct tatgcgtgct gatcacggcg taaaaaaggc
     4261 aaagggcgga tcgcggggtc gtaaaaacca cttaccccgc gataaccc
//
LOCUS       DAOPXN010000194         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20903_length_4259_cov_5.128925, whole genome shotgun sequence.
ACCESSION   DAOPXN010000194 DAOPXN010000000
VERSION     DAOPXN010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20903_length_4259_cov_5.128925"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="PKM49_08940"
     CDS             <1..519
                     /locus_tag="PKM49_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82958.1"
                     /translation="LYGPSSVWEATETLRQIDPLFSFTADVIVGFPGESERDFVETCD
                     LIQRIEPLKVHIFRFSPRKNTPAASFSGRVDEREKKRRSRALSLQVEMVSRQFREKHL
                     HLAREIVVETVNPSAEMMKGHDEFYILHSVYGDYRSMPKIITGEKAWVNPESIDRDHI
                     DGMVSRYVRVSQ"
     gene            500..1306
                     /locus_tag="PKM49_08945"
     CDS             500..1306
                     /locus_tag="PKM49_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNY82959.1"
                     /translation="MYVFLNDQWVKEEEAKISVFDKGFMSAESVYETLRTYQGRVFHL
                     EAHYTRLERSALEMKIPVSFTVKELKEIIEEGIRRNAIRAEAYVRVQITDKTVFVWVR
                     PMPAISPDIYANGVSVGFSPFRRRVIKKGDHTIKTTGALDIFLSRIHKPTELYDYILL
                     NEQGFLTEGTFSNLFFISGGRLRTPALACGVLSGITREVVIQIAQKAGIQVEEGWYNA
                     HELFAATEVFLSHTSASIVPVSRIEQFALPVGPLTRRLLNSFSEYIRTLA"
     gene            complement(1325..3511)
                     /gene="glgX"
                     /locus_tag="PKM49_08950"
     CDS             complement(1325..3511)
                     /gene="glgX"
                     /locus_tag="PKM49_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNY82960.1"
                     /translation="MPYLPLRGRPLPFGATPDEKGVNFSLFSKNAVNVIIELFQNFYD
                     ETPIFSYSFHPVHNKTGDVWHVYLEGVAPGAFYGYRVFGPYLPEFGHRFNPNKLLTDP
                     YAKANSGEYLWDHEYAYGYDKNAPAKDLSFSDIDSAKSHVKSIVIADDAFDWEDDKPL
                     RYPMKDTIIYEMHVRLFTKSCRELTKIADPDNHPDETASIAGTFDGILEKTDHLRRLG
                     VTSIELMPIFEFNPDANIHVHPETGKRLKNIWGYDPLAFFSVEASYTPGIGIGEQVRC
                     FKNFVKKMHKAGFEIILDVVFNHTGEGNHEGPTLSFRGIDNEIYYLLTPSDKRFYENY
                     SGCGNTFNCNHPVVKQLIIDSLRYWVTQMHVDGFRFDLAAILGRAPDGRWIGDVSLLK
                     DIAEDPVLAGTKLIAEGWDAAGGYFVGDFPKGWAEWNGKFRDAVRRFVRGDDGMVPEL
                     ATRLAGSSDLYNKSGRKPFDSINFITAHDGFTLMDLVSYSQKHNRLNGENERDGTNEN
                     YSHNHGTEGETQNPTILRLRVQQIKNFITLLNVSQGIPMVLMGDEMGRTQKGNNNAYC
                     HDDPTTWVDWSLKEKHREIWEFYSNMIHFRKRHECLRREHFFTGKRIGLGVRDITWHG
                     VKPFQPDWSHVSHTLAFVISGEDLDTFQARDNDIYVAANAFSSDLTFELPLMPRKEWH
                     RIVDTSLPFPYDFISESDAPLVRREYVVKARSVLLLISKRVMLPTS"
     gene            complement(3511..4098)
                     /gene="plsY"
                     /locus_tag="PKM49_08955"
     CDS             complement(3511..4098)
                     /gene="plsY"
                     /locus_tag="PKM49_08955"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HNY82961.1"
                     /translation="MGPLFIVLLYLSGSIPFSYIIPKTLKGVDIRRYGSGNVGATNVI
                     RSLGSKLGILCMVLDALKVYLPLILTQFALKDAPNKDLYLCLASVAGILGHDFPIFLK
                     FKGGKGVACTMGTFFALHWLTGVLFLLTAVSIALSTRIMSLASLTSLTLAVIFVFPIT
                     QNWSYFALYLFLCLFSFFQHRKNIVRLLQGKENKF"
BASE COUNT         1074 a         1050 c         1116 g         1019 t
ORIGIN      
        1 ctttacgggc cttcaagcgt ttgggaggcg acagaaacat tacggcagat tgaccccctc
       61 ttctccttta ccgcggatgt gatcgtaggg tttccggggg agagcgagcg ggatttcgta
      121 gagacttgcg atctgataca aaggatcgaa cccctgaagg tccatatctt ccggttctct
      181 ccccgaaaga acacgcccgc cgcatcgttt tcgggaaggg tagatgaacg agagaaaaag
      241 agaagaagtc gagcactttc tctccaggtc gaaatggttt ctcggcagtt tcgtgagaaa
      301 caccttcatc tcgcgagaga aatcgtggtc gaaacggtca acccgagcgc cgaaatgatg
      361 aagggccacg atgaattcta catccttcac tcggtatacg gtgactaccg tagtatgcca
      421 aagataatta cgggagaaaa ggcctgggtc aaccccgaat cgatagatcg ggatcatata
      481 gacgggatgg tgtcgcgata tgtacgtgtt tctcaatgat cagtgggtaa aggaagagga
      541 ggcgaaaatt tccgttttcg acaaagggtt tatgagcgct gaatctgtct atgaaaccct
      601 tagaacctat caaggccgcg tctttcattt ggaagcgcat tatacgcgtt tagaaaggtc
      661 tgcgctcgaa atgaagatac cggtctcctt cacggtaaaa gagctcaaag agatcatcga
      721 agagggaatt cggcggaacg ctatacgagc ggaagcttac gttcgggttc aaattaccga
      781 taaaaccgtc tttgtctggg ttaggccgat gccagccatc tctccggaca tttacgcgaa
      841 cggcgtttca gtcgggttct ctccctttcg tagaagggtg attaagaaag gagaccatac
      901 gatcaaaacg accggcgcgt tggacatctt cctttcgagg atccataaac cgacggagct
      961 gtatgactac attctactaa acgaacaagg attcttgacc gaggggactt tcagcaacct
     1021 atttttcatt agcggcggtc ggctacgaac cccggcctta gcttgcgggg tactatccgg
     1081 gatcacgaga gaggtcgtga tacagatcgc ccaaaaagca ggaatacagg tagaagaggg
     1141 ttggtataac gcacacgagc ttttcgccgc aaccgaagtg ttcttgtcac acaccagcgc
     1201 gagtatcgtg cccgtgtcgc ggatagaaca attcgccctc ccggtgggac ctttgactag
     1261 acgattgttg aactcgtttt ccgagtatat ccgaactttg gcttaaccgg cgcccaggta
     1321 tgttttagga agtagggagc ataacccgtt tagagatcag caggaggact gaacgcgctt
     1381 tcacgacgta ttctctccgg acgagcggag cgtccgattc gctgatgaaa tcgtacggaa
     1441 acgggagcga agtatcgacg atgcgatgcc actcttttct aggcatcaaa gggagctcga
     1501 aggtgaggtc ggaggagaag gcgttcgcag caacgtagat atcgttatcc ctggcctgga
     1561 aggtatctaa atcttctccg ctgatgacga aggccaaggt atgggagaca tgggaccaat
     1621 caggttggaa gggcttgacg ccgtgccagg tgatgtcccg tacccccaat ccgatacgtt
     1681 ttccagtgaa gaagtgctct ctgcgtaagc attcatgccg ttttctgaaa tggatcatgt
     1741 tggagtagaa ttcccaaatc tcacggtgct tttctttgag cgaccaatcg acccaggtcg
     1801 tcggatcgtc atgacagtac gcgttgttgt tccctttttg ggtacgtccc atctcgtctc
     1861 ccatcaggac catcgggatc ccttgcgaca cgttgagcag cgtgatgaag tttttgatct
     1921 gttgtacgcg taacctgagg atcgtcgggt tctgcgtctc cccttccgtt ccgtggttat
     1981 ggctgtaatt ttcgttcgtg ccgtcccgtt cgttttcacc gtttagccgg ttatgcttct
     2041 gggaataact aaccaagtcc atcagtgtga atccatcgtg cgcggtgata aagttgatac
     2101 tatcgaacgg ctttcttcca gacttgttgt atagatccga ggaaccggcc aatcgcgtgg
     2161 ccagttcggg gaccatgccg tcatcgccgc gaacaaaccg acgcaccgcg tctctgaatt
     2221 tcccgttcca ttccgcccac ccctttggaa aatctcccac gaaatacccg cccgctgcgt
     2281 cccatccctc ggctatgagc ttcgttcccg ctaagaccgg gtcttcggcg atatctttca
     2341 ggagcgagac gtcaccgatc caacgcccat ccggcgcgcg ccccagaata gccgctagat
     2401 cgaatcgaaa gccatccacg tgcatttgtg tgacccaata tcgcaaacta tcgatgatga
     2461 gttgcttcac gaccggatgg ttacagttga acgtgttccc gcacccggaa tagttctcat
     2521 agaagcgctt atccgacggg gtgagcaggt aatagatctc attgtcgatc ccccggaaag
     2581 atagggtggg gccctcgtgg ttgccttcgc ctgtatggtt gaaaacgacg tctaggatga
     2641 tctcgaatcc ggctttatgc atttttttca cgaagttttt aaaacaacgc acctgctccc
     2701 cgattccgat cccaggtgta taactggctt caactgaaaa aaacgcgagg gggtcgtacc
     2761 cccatatatt cttcaacctc tttccggttt cggggtgcac gtgtatgttc gcatccggat
     2821 tgaactcgaa tataggcatc aattcgatgg aggttacccc caatcgccgc aggtgatcgg
     2881 ttttttccag gatgccgtcg aacgtgcccg cgatagacgc ggtctcatcg ggatgattgt
     2941 cgggatcagc gatcttcgtg agttctcgac acgatttcgt aaagagtcgg acgtgcattt
     3001 cgtaaatgat cgtgtctttc atcggatatc gtaagggttt atcatcctcc caatcgaagg
     3061 catcatcggc gatgacgata ctcttgacgt gacttttcgc cgaatcgata tcggagaatg
     3121 acaggtcctt tgccggagcg ttcttatcgt agccataggc atactcatga tcccataaat
     3181 actctcccga gttggctttg gcgtaaggat cggtgagcaa cttattcggg ttgaaccgat
     3241 ggccgaattc cggcaaatac ggaccgaaga cccgatagcc ataaaaagct ccgggggcca
     3301 ccccttcaag ataaacgtgc cacacatcgc cggttttgtt gtgcacgggg tgaaaggaat
     3361 aagaaaaaat gggcgtctcg tcgtagaagt tttggaacaa ctcgataatc acgttgaccg
     3421 cgtttttaga gaaaagagag aaatttacac ccttttcatc gggtgtggcg ccgaacggca
     3481 aaggtcttcc acgaaggggt agatacggca ttaaaactta ttctctttgc cttgtagcag
     3541 gcgaacgatg ttcttacgat gctgaaagaa ggaaaaaaga cataagaaca gatacaaggc
     3601 aaaataggac cagttctgcg tgattggaaa gacgaagatc accgctaggg ttaaggaggt
     3661 caacgaagcc aaagacataa tacgtgtgct caacgcgata gaaaccgccg tgagcagaaa
     3721 caaaacaccc gttaaccaat gtaaggcaaa aaatgtgccc atcgtgcaag cgacaccctt
     3781 tccccctttg aatttcaaaa atatcgggaa atcatggcca agtatccctg cgaccgaggc
     3841 cagacagagg tagaggtctt tattcggggc atctttgagc gcgaattgcg tgaggatcag
     3901 cggcaggtat accttcaagg cgtcgagtac catacagagg attccgagtt tcgagccaag
     3961 gctccgtatc acgttggtgg cgccgacgtt ccccgagcca taccgtcgaa tatccacccc
     4021 tttcaaagtc ttggggatga tatagctaaa cggaatcgat ccggaaaggt acagcagaac
     4081 gatgaacagt ggacccatca cgactccccc ctgggaacga actttaagaa taaaggagac
     4141 cccgaaagtg ggttcagaaa cttccggaac tgtttcttca ccgccgtttt ataagaggca
     4201 ggaacgatct ggggatcatt cacgtggatg gcgatgatgg gaggcttatt cgccacttg
//
LOCUS       DAOPXN010000195         4257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20922_length_4257_cov_5.176107, whole genome shotgun sequence.
ACCESSION   DAOPXN010000195 DAOPXN010000000
VERSION     DAOPXN010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20922_length_4257_cov_5.176107"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKM49_08960"
     CDS             complement(<1..242)
                     /locus_tag="PKM49_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HNY82962.1"
                     /translation="MIGIICDTGSDIADRVRSNDRVEIVPLKVILDEKERLDTESGLS
                     EQVIEYQKRQQAKTSLPALEEVKAAFYRLIDRGYNE"
     gene            complement(404..1750)
                     /locus_tag="PKM49_08965"
     CDS             complement(404..1750)
                     /locus_tag="PKM49_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNY82963.1"
                     /translation="MSWQLLSVGFSKVDLVSAFLMALETLHANCVEADTILAVSELQW
                     DQAGVVIINGERLAQPPERARLSELQSSTRKKIVIIDQAQNTVPDDAQLTVFKPSIVY
                     RDSLTEMFEPIVFHYILSCLQYPKSDSTGSLSLSAASKEKMRQSIAELIHERETPDTD
                     GRRNSDFYLKPKVFDCFIDQSPQTVASWERTLTLTELVRQMDGYFIDPLPFADTGDRH
                     LRVQEDDEPFLINVRRTLFLWGFVYWTFLEGTELQAEDVYISKTTYEDRLFLVFSLSI
                     SGQEVEFYRERDLQRWPLIRSPGIVIQHELFLSESTLFISSAQSGAVRLSVWTPAKRV
                     ITEEGAPGDVSRTLSLLEGDTEELKELIRVAMRYLHEHLDAIGNSIVEQNATELGKAA
                     HKIKGSFSSFAAKRCIELAVEMERAAGENDFVRAKPTFNTLKVETDRLIEYLRQFL"
     gene            complement(1892..3304)
                     /locus_tag="PKM49_08970"
     CDS             complement(1892..3304)
                     /locus_tag="PKM49_08970"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HNY82964.1"
                     /translation="MERALLNRIALAQERMVRDLRDFVRIPSVSKDRDQVKAALAFYL
                     DRARQMGLKARSVLSDRVGVVEWGAGEETVGVLVHADVVGPGERSEWPCDPFEGMIAE
                     DRVFGRGTVDDKGPAISVLYALDAIRAVCPDPRKRVQIIIGTQEETEWSDIAAYAAAY
                     KLPDYGFTPDGEFPIANREKGYVDVEVSFPSAEIGGIEEYRLQELSGGHGANSIPDRA
                     RATVRGPGQALSRLKKRCEAISFMESEALVDGLRLTAKGRSAHSSLPHAGVNAIERLC
                     EVLATEPFAPSALAEAVRFLDAHREDPYGRRWGLYRLEDLVEGQYFDHTTIVPTRIET
                     KEGGVAITYNLRTVPPVDRRQIEAAFRERLLGFYGVTRILDYKDPLMVNPRYPFLEVM
                     ARAYEEVSGLPNSYRLAYGTSYAKAMDRFVSWGPLFPGDEDTAHQAGESLSITSWMNA
                     TRIYTLALARMVSSPDYLRG"
BASE COUNT          971 a         1259 c         1023 g         1004 t
ORIGIN      
        1 atctcgttgt aaccgcggtc gatcaatcgg tagaacgccg ctttcacctc ttccaatgcg
       61 ggcaaggagg tcttcgcctg ttgcctcttc tgatactcga tgacctgttc agaaagcccg
      121 ctttcggtgt ccaaacgctc tttctcgtcc aagataacct ttaatgggac gatctccaca
      181 cggtcgttcg accgtactct atcggcaata tcgcttcctg tatcacagat tattcctatc
      241 atagtgacct ccatatgacc ggcccaccct atcgctcacc tttcctacta tacttccgta
      301 gaaacggacg ggccgaagcg gatcctttgc ccctattgta ctatatttac ccccaaaata
      361 ccgcaaaaac tctccaaagc gcccacgccc ctggaaggtg aattcatagg aattgacgca
      421 agtattcgat taatctgtcg gtttcgacct tgagtgtgtt aaaagtgggt ttcgcccgca
      481 cgaagtcgtt ctctccggca gcccgctcca tttccacggc cagctcgatg catcgctttg
      541 cagcgaagct agaaaagctt cccttgattt tgtgagccgc tttcccgagc tcggtggcgt
      601 tctgttccac tatgctgttg ccaatcgcat ccaggtgttc gtgcaagtac cgcatcgcca
      661 ctcgaatcaa ttccttcagc tcttcggtgt ccccctccag taacgaaagc gttcggctga
      721 catcccccgg cgccccttcc tcggtgatga ctctcttcgc cggagtccat acgctgagcc
      781 ttacagcgcc cgactgggcg ctcgaaataa aaagcgtcga ctcactgagg aaaagctcgt
      841 gttggatgac aattccaggc gaacgaatca ggggccatct ctgaaggtcc cgctctcgat
      901 agaactcgac ctcttgcccg cttatagaaa gcgaaaacac gagaaaaagg cggtcttcat
      961 aagtcgtttt cgaaatgtag acgtcttcgg cctgtaactc cgtaccttcg agaaaagtcc
     1021 aatagacaaa accccataaa aagagggtac gcctgacgtt gatcagaaat ggttcgtcgt
     1081 cttcttgcac gcgaaggtga cgatcacccg tgtcagcgaa cggcaagggg tcaataaaat
     1141 acccgtccat ttgtcgaacc aactccgtca gcgttagcgt gcgttcccag gatgcgacgg
     1201 tttggggaga ttgatctatg aagcaatcaa atacctttgg cttcaaataa aaatcactgt
     1261 tgcgacgacc gtccgtgtcg ggagtttctc gctcatgtat taattcagct atactctgtc
     1321 tcattttttc ttttgaagcg gcggatagtg aaagagaacc ggtcgagtct gattttggat
     1381 actgcaagca agaaaggata tagtggaata cgatgggctc aaacatctcg gtgagtgaat
     1441 cgcgatagac gatcgaaggc ttgaacactg taagttgagc gtcgtcaggc acggtattct
     1501 gggcttggtc gatgatgact attttttttc gtgtagacga ttgaagttca gacaaacgcg
     1561 cgcgctcggg cggctgcgca aggcgttcgc cattgatgat gaccacgccg gcctggtccc
     1621 attgtagctc ggagactgcc aaaatcgtat cggcctccac acagttggcg tgaagtgtct
     1681 ccaacgccat caaaaatgcg cttacgagat ctactttcga aaaacccaca gacaataact
     1741 gccagctcac tttgccgcac ctccaagcta taagcgaaaa atgcgcaatc cctttttcac
     1801 cctccacgac gggcacatta gggtttgcga atacactttc tatccaaact catcgtcaac
     1861 cagccccgag ataaaaccgg tactagattt gtcaaccgcg taaataatct ggtgaggaga
     1921 ccatccgagc gagagccaac gtgtagatcc gggtggcatt catccacgag gttatggaga
     1981 gcgactctcc cgcctgatgc gcggtatctt cgtccccggg aaagaggggg ccccagctca
     2041 cgaaacgatc catcgccttg gcgtaagagg tgccgtaggc gagcctgtag gagttgggca
     2101 gtccggatac ctcttcataa gcgcgcgcca taacttcgag aaacggataa cgtgggttca
     2161 ccatcaacgg gtctttgtaa tcgaggatcc gggtaacacc gtaaaaaccg agtaggcgtt
     2221 ccctgaaagc ggcttcgatt tgccgacgat cgacgggagg aacggttcgg aggttgtagg
     2281 tgatcgccac cccgccttcc ttcgtctcta tacgtgtggg gacgatggtc gtatggtcaa
     2341 aatactgccc ctccacgagg tcctctaagc gatagagtcc ccaccgccga ccgtatggat
     2401 cctccctgtg ggcatccaaa aacctcaccg cctcggcgag cgcggatggg gcaaagggct
     2461 ccgtggcgag gacctcacac aaccgctcga tcgcattcac accggcgtgc ggaaggctcg
     2521 aatgggccga acgcccttta gccgttaaac gtaggccatc gacgagcgcc tcgctctcca
     2581 taaacgaaat cgcttcacac cgtttcttca ggcggctcaa cgcctgcccc ggcccgcgga
     2641 ccgtagccct cgcccggtcc ggtatagaat tggccccgtg cccgccggac agttcctgca
     2701 ggcgatactc ttctattccg cctatttcgg cgctaggaaa agagacctcg acatccacgt
     2761 aacccttttc gcgattcgcg atcggaaact caccgtcggg agtgaaccca taatccggaa
     2821 gtttataggc agcggcgtag gccgctatat ccgaccactc ggtctcctct tgcgtaccga
     2881 tgatgatttg tacccttttt cgtggatccg ggcagacggc gcgaatagcg tccaacgcat
     2941 acaacaccga gatcgcgggt cccttatcgt cgacggttcc tcgcccgaat acccgatctt
     3001 ccgcgatcat cccctcaaaa gggtcgcagg gccactcgct gcgttcgccc ggccccacga
     3061 catccgcgtg gaccaatacc ccgaccgttt cctctcccgc accccattcg accacaccca
     3121 cgcgatccga gagcacacta cgcgccttta atcccatctg ccgggcgcga tcgagataaa
     3181 atgctaatgc ggccttcacc tggtcgcgat ccttagaaac gctgggaatc ctcacaaaat
     3241 ctcgcaagtc cctgaccatt cgctcttgtg cgagcgcgat acgattcaat aaagcccgtt
     3301 ccacaacccc tccttctcgg gcgctctttt ccagatagcg cctctctggg aactctggga
     3361 gcgccggact tcagtccggc atgtcttcgg ttttttagta acgccccaag aacgcctttc
     3421 cgggcacgcc gaactactgg gaccgcctgg gacgccttgg gagcgccgaa cttcagtccg
     3481 gcaggttttt gcttttccgg gagcaccgga cttcagtccg gcatatcttc tgcttttgcc
     3541 tttctgggac caccctggga cggccgaact ttagtttgac atgtcttatg ctttctcgga
     3601 ccgccgaact tcagtccggc atggtttcag gttttggctt ggctctccca taaattcacc
     3661 gcgtataatc ccgggcacaa cttcctcacc caaagcttag cggtcaaata actcgacccg
     3721 ccctctattc acaaaacaat aataccatga taacgacagg aaacgccaac tttgtctcac
     3781 caacgaaaaa gcaacccgga cacgccatcg gaacgccttc ggaacgccag gttttaagtt
     3841 ggcttgtctt ctacgtttac attcctcctc cgtggtctct gtgttctctg tgcgagacct
     3901 tccaatttct ttttaatctt gttttgattg aaaaggcatg ccgaactaaa gttcggagtt
     3961 cccagaaaat cttcctccgt gccctctgtg ttctccctgc gaaaccttcc tttttacttt
     4021 tggccatttt tacaacgaaa agccttgccc tactaaagtg cggcgcgccc agaaagacgt
     4081 gccaaactaa accgcggcat atcctttttt ttcggcgtta caaccggtcc tcgctcgtta
     4141 gcgcaacgaa aagggagccc tctcggattt accacataca gacaaagacc atgggttttg
     4201 cggtataatg gattcgagcg gccccgcatg aaccagaggg tagcgggaca taaggag
//
LOCUS       DAOPXN010000196         4247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_20994_length_4247_cov_4.247615, whole genome shotgun sequence.
ACCESSION   DAOPXN010000196 DAOPXN010000000
VERSION     DAOPXN010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20994_length_4247_cov_4.247615"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1334)
                     /gene="glyA"
                     /locus_tag="PKM49_08975"
     CDS             complement(69..1334)
                     /gene="glyA"
                     /locus_tag="PKM49_08975"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNY82965.1"
                     /translation="MWNNLRQEDSEVYAIIKQELARQEHGLELIASENFVSKGVMEAM
                     GSILTNKYAEGYPKKRYYGGCVYVDMAETLARERVKELFHAKYANVQPHSGSQANMAA
                     YFAVADPGSVLMGMALSHGGHLTHGAPVSFSGKIYRVVQYGVNIETEKIDYDEVEKMA
                     LEHNPKILVAGGSAYSRIIDFKRFRQIADRVGAYLVVDMAHFAGLVAAGIYPNPLDYA
                     HIVTSTTHKTLRGPRGGLILTNDEELYKSINKTIFPGIQGGPLMHIIAAKAVCFKEAL
                     SPDFVDYQKRVVANAVRLAEELEKLGVRIVSGGTDTHLFLVDLSPKNATGKAVEAALE
                     TAHVTVNKNTVPNEKLSPFVTSGIRVGTPAVTTRGMGPSEMVEIAKIIQEVIDHMDTE
                     GQLPESIREAVIGRVDALCERFPLYQELL"
     gene            complement(1351..1815)
                     /locus_tag="PKM49_08980"
     CDS             complement(1351..1815)
                     /locus_tag="PKM49_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNY82966.1"
                     /translation="MMMKSYSVYVNGKAYEVQVEELGAAVAPQMPVAVTASLQEARPA
                     PARIEPQPTPPSAPPQEPQSSKPAAPATVKAPSPKGQKGKEYHAPMSGLIIDVFVKPG
                     QSVKTGDALLRIEAMKMENDIICESNGVVEEVFIHKGENVETGKNMVTVIPS"
     gene            complement(1815..2186)
                     /locus_tag="PKM49_08985"
     CDS             complement(1815..2186)
                     /locus_tag="PKM49_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HNY82967.1"
                     /translation="MEVISLTIVGISVVFLCFVFIYVLLKAFEAIFRERKPEREEPAL
                     TKPVIGTDGGFSSISSLAVGGTGDAVITEEEVAAIGAALFAYLDKPYRLVGLRRVSQE
                     ETRCRTPRKYKTWKVHQKGDK"
     gene            complement(2195..3748)
                     /locus_tag="PKM49_08990"
     CDS             complement(2195..3748)
                     /locus_tag="PKM49_08990"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNY82968.1"
                     /translation="MNDMDSLNQAFIEKRNAVYEGGGPKAVEKQHQAKKMTARERIHQ
                     LLDKGSFFEIDTFVQHRCVNFDLKDKTIPEDAVITGFGNIGDKKVGVFSQDFTVMGGS
                     LGEMHAKKIMKIQDLCLKMGIPLIGINDSGGARIQEAVDALFGYGGIFFRNTRSSGVI
                     PQISIIMGPCAGGAVYSPAITDFVIMVDQVAKMFITGPQVIKAVTGEDVSQEELGGAK
                     VHNQKSGNAHFLAVNDEEALEICRRLFDYIPSNNTEFAKTIPLETVNLDNLADVGKMI
                     PVDGKKGYDVKQVINGIVDRESFMEVHALYALNIVVGFARLGGIPIGVVANQPSQWAG
                     SLDINASDKAARFIRFLDAFNLPIVTLVDTPGFLPGTAQEFNGIIRHGAKLLYAYSEA
                     VVPKITVILRKAYGGAYIAMGSQHLGADFVCALPTGEIAVMGPEGAANIIFKKEIDQS
                     ENPEQLRTEKIEQYRAQFANPYVAASRGYIDQVISPDEMREAIYQNLTLAKNKVEPYP
                     DKKHGNIPL"
     gene            complement(3764..4174)
                     /gene="mce"
                     /locus_tag="PKM49_08995"
     CDS             complement(3764..4174)
                     /gene="mce"
                     /locus_tag="PKM49_08995"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HNY82969.1"
                     /translation="MAISHIDHIGIAVSSLEKVKPFYEEGLGIRVEEVEELPQKHLKV
                     AFIPVGDTRIELLEPTSEASTVHQFIQKRGEGIHHIAFHVDDIEASIAQLKEMGYRLL
                     SEVPEEGAGGTKIVFLHPKSGFGVLIELVEGEGH"
BASE COUNT          881 a         1192 c         1034 g         1140 t
ORIGIN      
        1 cgaaaagact catagatgaa aaaaaaggcg gatccctccg ccttccctaa ccggggaaac
       61 cgaaagttct acaatagctc ctgataaagc gggaaccgtt cgcacagcgc gtcgacgcgt
      121 ccgatgaccg cttcccgaat actctccggg agttgccctt ccgtgtccat atgatcgatc
      181 acctcttgga tgatcttcgc gatctccacc atctccgagg ggcccattcc ccgtgtcgtc
      241 acggccggag tgccgacccg gataccactg gtgacgaacg gcgagagctt ttcgttgggg
      301 acggtgtttt tgttaacggt tacgtgggca gtctccaaag cggcttccac cgcttttccg
      361 gtcgcgtttt tcggactgag gtctaccaaa aacaggtgtg tgtcggtccc gcccgaaacg
      421 atacgaaccc caagtttctc caattcttcc gccaagcgaa ccgcgttagc gaccacccgt
      481 ttctgatagt ccacgaaatc aggggagagc gcctctttga agcaaacggc tttagccgcg
      541 atgatatgca tcaaaggccc cccttgaatg cccgggaaga tcgttttatt gatgcttttg
      601 tagagctctt catcgttggt gagaatcaaa ccgccgcgcg gtcccctcaa ggttttatgg
      661 gtcgttgagg tgacgatatg ggcataatcc agcgggttcg ggtatatccc tgccgctacg
      721 agaccggcga agtgcgccat atcgacgacc agataggcgc ccacgcgatc ggcgatctgt
      781 ctgaaccgtt taaagtctat aatcctcgaa taggcgctgc caccggcaac caggatcttc
      841 gggttgtgct ctaaggccat cttctccact tcatcataat ctatcttttc cgtctcgata
      901 ttgaccccat actgaacgac acggtagatt ttaccggaaa agctgaccgg agcgccatga
      961 gtgaggtgcc ctccgtggct aagcgccatt cccatcagta cgctacccgg gtccgccacg
     1021 gcaaaatagg cggccatatt cgcctgcgat ccggaatggg gttgtacgtt ggcgtatttg
     1081 gcgtggaaaa gctctttcac gcgctctctc gccagagttt cggccatatc cacgtagacg
     1141 cacccaccgt agtatcgctt cttgggataa ccctccgcat acttgttcgt caatatgctc
     1201 cccatcgcct ccataacgcc tttcgaaacg aagttctctg aggcgatgag ttccaaaccg
     1261 tgttcttgac gcgccaattc ttgcttaatg atcgcgtata cttcgctgtc ttcttgtcgt
     1321 aggttattcc acacgattgt catctcaccc ttatgatggg atcacggtaa ccatattttt
     1381 tccggtttct acgttttcgc ccttgtgaat gaaaacctct tctacgacgc cgttgctttc
     1441 acagatgatg tcattttcca ttttcatcgc ttctattctc agcagggcat ccccagtttt
     1501 aacggattga ccgggtttga cgaatacgtc gatgatgagc cccgacatcg gcgcgtgata
     1561 ctccttcccc ttttgccctt taggcgatgg ggcttttaca gttgcaggag ccgctggttt
     1621 ggagctctgt ggttcttgtg gaggtgccga tggtggggtg ggttgcggtt cgatccgagc
     1681 cggagcaggg cgggcttctt gcaatgacgc agtcacggcg accggcatct gcggcgcgac
     1741 tgctgcccct aactcttcca cttggacttc atatgctttg ccgttcacat agacactata
     1801 actcttcatc atcattattt gtcccctttc tgatgaactt tccaagtttt atacttcctc
     1861 ggcgtccggc atcgcgtttc ttcttgtgag acccgtctca agccgacaag cctgtagggt
     1921 ttgtctaaat aagcgaataa ggccgcgcct atcgcggcca cctcttcctc ggtgattacg
     1981 gcatctcccg taccacccac cgctagggaa gaaatggagg agaatccccc gtcagtcccg
     2041 atgaccggct tcgtcaaagc cggctcctct cgttctggtt ttcgctctct gaatatggct
     2101 tcaaatgcct tgagcaggac gtatatgaat acaaaacaga gaaagactac ggatattccg
     2161 acgatggtta ggctgatgac ttccataacc tctcctataa cggaatgttc ccgtgttttt
     2221 tgtcggggta gggttccacc ttatttttcg cgagcgttag gttttggtag atcgcctctc
     2281 tcatctcatc cggtgagatc acttgatcga tataccctct ggaagcggcg acatagggat
     2341 tcgcaaattg cgcccggtat tgttcgatct tttcggttct gagttgctcg gggttttccg
     2401 attggtcgat ttcttttttg aagatgatgt tcgcggcgcc ttcgggtccc atcacagcaa
     2461 tctccccggt cggcaacgcg cagacgaaat ccgcccccaa atgttggctg cccatcgcga
     2521 tgtatgcccc cccgtacgct ttccttaaga tcaccgtaat cttcggcacg acggcctcgc
     2581 tgtaggcgta gagtagcttc gccccgtgac ggatgatccc gttgaactct tgcgcggtac
     2641 cgggtaagaa gcccggcgta tcgaccaggg tcacaatggg tagattgaag gcatccaaga
     2701 agcggatgaa tcgcgcggct ttgtccgagg cattgatatc gaggctgccg gcccattgac
     2761 tcggctggtt cgcgacaacc cctatcggaa tccctccgag gcgggcaaat ccgacgacga
     2821 tattgagcgc gtaaagcgca tgcacttcca taaagctctc ccggtcgaca atgccgttaa
     2881 tcacttgctt gacgtcgtaa ccctttttcc cgtctaccgg tatcatcttt ccgacatcgg
     2941 ccaggttatc gaggttgaca gtttccagcg ggatggtttt agcgaattcg gtgttgttgg
     3001 atgggatgta atcgaaaagc cttcgacaaa tttctaacgc ttcttcgtca tttaccgcta
     3061 aaaagtgggc gttaccgctt ttctggttgt ggaccttcgc gcctcccaac tcttcctggg
     3121 acacgtcttc tcccgtcacg gccttaatga cttggggacc tgtgataaac attttggcga
     3181 cctgatcgac cataatcaca aaatcggtga tcgcgggaga gtagaccgcg ccgcccgcgc
     3241 agggccccat aatgatcgag atttgcggta ttactccgct tgaccgggtg ttgcgaaaga
     3301 agatgccgcc atatccaaac aaagcgtcca cggcttcttg gattcgagct ccaccggaat
     3361 cgttgattcc gatgagtgga attcccattt ttaggcagag gtcttgtatc ttcatgatct
     3421 ttttcgcgtg catctctcca agagagccgc ccatcaccgt aaagtcttgg gaaaacaccc
     3481 cgactttttt atctccgatg ttcccgaaac ctgtgatgac cgcgtcttcg gggatggttt
     3541 tatccttaag gtcaaaattg acgcaccggt gttggacgaa cgtgtctatc tcaaagaaac
     3601 tgcccttgtc cagcaattga tggatccgtt ctcgagccgt catttttttc gcctgatgtt
     3661 gtttttcgac ggctttcggc ccaccgcctt catacacggc gttgcgcttt tctatgaaag
     3721 cctgattcag tgaatccata tcgttcataa accttctcct ctcttagtgg ccttctcctt
     3781 cgaccaactc tattaagacc ccgaagccgc tcttcgggtg caaaaaaacg atctttgtcc
     3841 cgcctgcgcc ttcctccgga acctcgctca gcaaccggta gcccatctcc ttcaattgag
     3901 cgatcgacgc ttcgatatca tccacgtgga acgcgatgtg atgaatgccc tctcctcgct
     3961 tctgtataaa ctggtggacc gtggaggctt cggatgttgg ctccaaaagc tcgatgcgcg
     4021 tatcgcccac gggaatgaag gccactttta gatgtttttg aggtaactcc tccacctctt
     4081 ctacacgaat acccaaacct tcctcataga aaggtttgac cttttctagg gacgaaaccg
     4141 ctatcccgat atgatcgata tgagatatcg ccatcgttac ctccttttgc ggaaaagccg
     4201 cgttttcttt ggccctttcg tttagaatta taacatacgg tcggtgg
//
LOCUS       DAOPXN010000197         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_21003_length_4245_cov_4.597613, whole genome shotgun sequence.
ACCESSION   DAOPXN010000197 DAOPXN010000000
VERSION     DAOPXN010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21003_length_4245_cov_4.597613"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PKM49_09000"
     CDS             complement(<1..511)
                     /locus_tag="PKM49_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82970.1"
                     /translation="MSIFSKRMNGDIGLTKKTSLWRKNSRTVRDEEEIVRRSLAISIY
                     EGIGAVIYALVVQGVFFTKIALEFGADAFTLSLLLGIQYLSQVFQLIVPTMIARFGKR
                     KPFVLTTVFLVRSLWLAILILAIMGVTRRDLFVIIVSCGFVCSALVGNAWTSWMRDLV
                     PQRRMGSFFG"
     gene            1337..2152
                     /gene="rpsB"
                     /locus_tag="PKM49_09005"
     CDS             1337..2152
                     /gene="rpsB"
                     /locus_tag="PKM49_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNY82971.1"
                     /translation="MPVVSMKQLLEAGVHFGHRTMRWNPKMKEYIYTDRKGVHIMDLQ
                     KTLKLLDQAYYFVRDEARKGATILFVGTKRQARKAIQEEAERCGGFFVNNRWLGGMLT
                     NFPTIRKRIEKLKELENYVESPEFEKMPKPQQAIVKRQLEKLQKNLGGVKEMKEVPDI
                     LFIIDPRKEQTAVLEANHLGVPIVAPVDTNCDPDEIDYIIPGNDDAIRAIKLITGKIA
                     DAYLEGREGLSEAMAQKAATASVAETAGSASSIADDETEEGDRASSNTTGDAF"
     gene            2284..3291
                     /locus_tag="PKM49_09010"
     CDS             2284..3291
                     /locus_tag="PKM49_09010"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNY82972.1"
                     /translation="MGQAKQRKTSLAFMAFSITLLVWTVTFFGAYTLPSQVSKKTTSS
                     FGECRNSNDLTYTRFHAGIDLSTNGLENTNIYATEEGYLWKIWVNHEDYGNAIYIMHA
                     DGIASVYGHLNRFSDRFLPVIQSAMIEFGEATYMEITFSPNEYTIRRGETIGLSGQTG
                     TALAPHCHFEFIDVRHEEYINPLLYLRDLLDPPLADLKIERVRIDGEALSIVENGTLY
                     FTSAIPKIEINTRLSNRTGQGKYGVKAITFYVNEAEFYKILFDRIPRKMLENGDVVFG
                     EGSNHSNYWYKLYASLQEKPIVVNLWKNLSTFPDLAACRIIVEDDWGNVKTWNFKLRR
                     K"
     gene            3291..3620
                     /locus_tag="PKM49_09015"
     CDS             3291..3620
                     /locus_tag="PKM49_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82973.1"
                     /translation="MDVKCWFWKRVKKPKYITSQNELLIFYAQCGTCGELFRTIIRKN
                     SELSQNFEHENTYEVHKELIGSYCPNRIQVHLWLSQGLKLLKQTVTGGILLTEEEYTR
                     LKEEKNA"
     gene            3617..>4245
                     /locus_tag="PKM49_09020"
     CDS             3617..>4245
                     /locus_tag="PKM49_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNY82974.1"
                     /translation="MRRLFSSESVTEGHPDKVADQISDAILDALIEKDPESRVAVETI
                     VTRGLAFVSGEITTRGYVDLPKVIRETIWNIGYTRAKYGFDGETCGVLSSIAEQSEDI
                     ALGVNQSLEVKGGEKAEEELDQLGAGDQGMMFGYATNDTPEMMPLPILLAHKLAMQLA
                     KTRKDGTLHFLRPDGKSQVTIEYDDQWKPLRVDTVLIAAQHEDISYAEIR"
BASE COUNT         1108 a         1096 c         1088 g          953 t
ORIGIN      
        1 atccaaagaa gctccccatc cttctttgag gaacgaggtc cctcatccag gaggtccagg
       61 cgttcccaac caacgcgctg cagacgaacc cgcaagagac aatgatgaca aaaagatctc
      121 gacgggtcac acccataatc gccagtatca gaatggccaa ccacaagctt cggaccaaga
      181 aaacggtcgt gagaacgaaa ggtttccgct ttccgaacct cgcgatcatt gtggggacga
      241 tgagctgaaa gacctggctc aggtactgaa tacccagcag caagctcaag gtgaaggcgt
      301 ccgcgccaaa ctccagcgcg atcttcgtga agaaaacgcc ttggacaacc agcgcgtaga
      361 tgaccgctcc gatcccttca tagatcgaaa tggccaagga gcgtcgaacg atttcttcct
      421 cgtcccgaac cgttcggctg ttttttctcc ataaagaggt tttcttcgtg agacctatat
      481 ctccgttcat tcttttcgaa aagatactca aaagactttt cacgcctttc ttcgactttc
      541 gccagattgt accatacgcg ctgattattc cgtatacgtt ttaagacgct atcgataacg
      601 ctgttccggg agcggcgagc aatagtttgg catgtgttcg gagtttgctc ctctgggaac
      661 gcggaacttc tcggagcgct acacttaagt gtggcatgtg ttgtgttctc tccacgccct
      721 ccgtgagaaa tcaccttcca ttcgtaaaaa cctgccaatc ttaagattgg cgttcccaga
      781 gcgttcccaa ccgcagcatc gcggcgtaag cgccacttct tctcgcgacg gcgcgaaacg
      841 cttttaagaa agacaaagag cggatcgcgg ggtcgtaaaa atcacttacc ccgcgataac
      901 ctccgaaaac aactttcaga gcatttcctt tttaccgata tagatgatct cctttcccat
      961 ctttcccatc tttccctcgt attctccgtg cgaacccctc ttttttcttt ttaagggtaa
     1021 gccaaactaa agttcggcgt tcccagggcg ttcacaacga tcttttctct gtgttctctg
     1081 tgccctccgt gccctccgtg cgaacccttc tttttttaaa gacatcccga actaaagttc
     1141 ggcgttcaca gggcgttcac aaatggcgtt cccagcttcg gtgttgattt tcttatcggt
     1201 atttgctata ataccgcgaa tctctcacac gatgccgctc cttttctggt gcccgtacgg
     1261 cgacaggcta cgggttggaa agcgcggcgc ttgccgcaga ggatcgtgga ggaacaacct
     1321 atatggaggt gcagtaatgc cagtcgtttc tatgaaacag ctccttgaag ccggagttca
     1381 tttcggtcat cgaacgatga gatggaaccc gaaaatgaag gagtacatct acaccgatcg
     1441 taagggcgtc catattatgg acctacagaa gacgctcaaa ctactcgatc aagcttacta
     1501 tttcgtccgc gacgaagccc gtaagggggc cactatcctg tttgtcggga cgaaacgcca
     1561 ggcgaggaag gctatccaag aagaagccga gcgttgtggc gggtttttcg tgaacaatcg
     1621 ctggttggga gggatgttga ccaatttccc gacgattcga aaaagaatcg agaagctgaa
     1681 agagcttgag aactacgtgg aatcacctga attcgaaaag atgcccaaac ctcagcaagc
     1741 catcgttaaa aggcagctcg aaaagttgca gaaaaacctg ggtggcgtga aagagatgaa
     1801 agaggtcccg gacatcctct ttatcatcga ccctcgcaaa gaacagacgg ccgtgttgga
     1861 agccaatcac ctcggggttc cgatcgtcgc gccggtagat acgaactgtg accccgatga
     1921 gatcgactat atcatacccg ggaacgacga cgcgatacga gcgatcaagc tgatcacggg
     1981 aaagatcgcg gacgcctatc ttgaagggcg cgaagggctt tcggaagcga tggcccagaa
     2041 agcggcaacc gcttccgttg cggaaactgc cggttccgcc tcttctattg ccgatgacga
     2101 aaccgaagaa ggggaccgcg cgagttcgaa taccacgggc gacgcgtttt agccgctctc
     2161 gagtccgttg agaccccccg gaacaagcgg tgacgatgcc ggtttcggga cgggacgatt
     2221 ccatcaggcg cgatacagct tttgcgtcct gatggtatca tccaatggaa agcaggtgac
     2281 cttatgggac aggcaaagca gagaaagacc tctctcgcct ttatggcgtt ctctataacg
     2341 ttgctcgtgt ggacggtgac gttcttcgga gcctacacgc tcccctctca agtctccaaa
     2401 aaaactacgt cgtctttcgg ggagtgtcgc aattcgaacg acctgaccta cacgaggttt
     2461 cacgccggaa tcgacctatc caccaacggg ctcgaaaaca ccaacatcta tgcgaccgaa
     2521 gaggggtatc tctggaagat ctgggtgaac cacgaagact atgggaacgc gatttatatc
     2581 atgcacgcgg acggtatcgc ttccgtttac ggtcatttga accggttttc agatcgcttt
     2641 ttaccggtga tccaatcggc gatgatcgaa ttcggggaag ccacctatat ggaaatcact
     2701 ttcagcccga atgagtacac gattcgacgg ggcgagacca tcggcctctc cggccaaact
     2761 ggaacggccc tcgcgcccca ttgtcatttc gaattcatag acgtgcgaca cgaagagtat
     2821 atcaatccgc tcctctactt gagggaccta ttggatcccc cgcttgcgga ccttaagatc
     2881 gaaagggttc gtatcgatgg agaagccctg agtatcgtcg aaaacggaac cctatacttt
     2941 acttctgcga tcccgaagat agagatcaat acccggttgt cgaatcggac gggacaaggg
     3001 aaatacgggg ttaaggctat taccttttac gtcaacgagg ccgaattcta taagatcctt
     3061 ttcgatcgta tcccacgtaa gatgctcgag aatggcgacg tcgttttcgg agaggggtcg
     3121 aatcattcaa actattggta taaattgtac gcctctctcc aagagaaacc gatcgtggtc
     3181 aatctatgga aaaacctgtc gaccttcccg gatctagccg cctgtcggat catcgtcgaa
     3241 gacgattggg gcaacgtcaa aacgtggaat tttaaactga gaaggaagtg atggacgtga
     3301 aatgttggtt ttggaagcga gtgaagaaac cgaagtacat cacctcccaa aacgaactgc
     3361 tcatttttta cgcgcaatgc ggaacctgcg gggagctctt tcgaactatt atccgaaaga
     3421 actctgaact gtcgcaaaat ttcgaacacg agaacaccta tgaggtgcac aaagagctta
     3481 tcggcagtta ctgccccaat cggatacagg tgcatttgtg gctcagtcag gggctgaagc
     3541 ttttaaaaca aacggtcacc ggagggatat tactcaccga agaggagtat acgcgattga
     3601 aggaggaaaa gaacgcatga gacggttatt cagcagcgaa agtgtaacgg aagggcatcc
     3661 ggataaggtc gccgatcaga tctccgacgc catcttggat gcgctcatcg aaaaagatcc
     3721 ggaaagccga gtcgcggtcg aaacgatcgt gacgaggggt cttgccttcg tgtccggaga
     3781 gatcaccacc cgtgggtacg tcgatctccc taaagtcata agagaaacga tatggaatat
     3841 cgggtacacc cgagcgaaat acggatttga cggagaaacg tgcggtgtgc tatcttcgat
     3901 tgcggagcaa tcggaagata tcgcgctcgg agtcaaccaa tccttagaag tcaaaggagg
     3961 ggagaaggcg gaagaagagc tggaccagtt gggcgccggc gaccagggta tgatgttcgg
     4021 ttacgcgacc aacgatacgc ccgaaatgat gccgcttccc atacttttag cccataaatt
     4081 ggcgatgcag ttggccaaaa cccgcaagga tgggaccctg catttcttgc ggcctgacgg
     4141 caagtcccaa gttacgatcg aatacgacga ccagtggaaa cccttaagag tggacaccgt
     4201 tttaatcgcc gcgcaacacg aagacatttc gtatgccgaa atccg
//
LOCUS       DAOPXN010000198         4185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_21423_length_4185_cov_6.025182, whole genome shotgun sequence.
ACCESSION   DAOPXN010000198 DAOPXN010000000
VERSION     DAOPXN010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21423_length_4185_cov_6.025182"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="PKM49_09025"
     CDS             complement(<1..696)
                     /locus_tag="PKM49_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNY82975.1"
                     /translation="MLIGIIAIAILVMVFAGTGIRIVRPFQKGLVERLGKYNRIADSG
                     IHFIIPFFERMTMVDMREQVIDVPPQEVITKDNVVVAVDAVIYYMITDAYQVIYNVAF
                     FQVAAIKLAQTNLRNVIGELELDQTLTSREMINAKLREVLDDATDKWGVKVTRVEIKK
                     IDPPQDIMDAMSRQMKAERTKRAAILEADGIKQSEILRAEGSRQAKILEAEGQAESIK
                     LVAEAERFKLLTEA"
     gene            complement(713..1153)
                     /locus_tag="PKM49_09030"
     CDS             complement(713..1153)
                     /locus_tag="PKM49_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNY82976.1"
                     /translation="MKTTIFWLILGVLFLIAEIFTPSFFIMWFGIGAFAAMLVAFLFE
                     NSVLYQSIAFILVSLILVLFTRKLAQKMSGKPSRLIVQDEMIGRIGVVTETITADGGK
                     GFVRIDKEIWRARSRSEQEIPVGAKIKVIELKGVTVTVDPVEIK"
     gene            complement(1150..1851)
                     /locus_tag="PKM49_09035"
     CDS             complement(1150..1851)
                     /locus_tag="PKM49_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNY82977.1"
                     /translation="MTALRRALDVGRTFRVTTMNAQIAYYYLTMPEYQVALKGTLVIP
                     DGVGLSWAIRKRLNTRVSRFPGVELGFELCRLAGKSGESVFLFGSKPGVAEKAATFLT
                     QETGVRIAGYRHGFHNPDDDTQLGICQEISESGASILFVALGAPKQEIWLERFFEKTN
                     ARLGIGVGGSLDVWAGVVKRAPGWIQRMNMEWLYRILQSPRKKFKVVLQLLKFISIVN
                     ANGISGSIEEKRRFE"
     gene            complement(2743..3657)
                     /locus_tag="PKM49_09040"
     CDS             complement(2743..3657)
                     /locus_tag="PKM49_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNY82978.1"
                     /translation="MITSVPIVPEPNRPHWYGRGLSLSEDLVPRINNSNAKEWMALLI
                     LQKTLSAFVELPGLLILILLAFTLFAKMSRGWRRVLSVLIVVFYLCSAGFFSQWLVAP
                     LENHFATSYAQEVGVDASQSAIVILSSSHRVGVPSGYGGEVTNEPDRTTALRLNRGFS
                     LYRERPMPIIVSGGPLWSSHQVHVAETMAETLAMWGVPEKDIVIEDRAMTTDQNAAYS
                     LALLDEAVATVYIVTSAIHLPRAMLAFEREAKKRSSPLWLVPIPTDFYIEERETIWLD
                     FLPSSGAFTATAAALHEWFGLLFYKWFK"
     gene            complement(3734..>4185)
                     /locus_tag="PKM49_09045"
     CDS             complement(3734..>4185)
                     /locus_tag="PKM49_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82979.1"
                     /translation="TENGVLELEKSYNAFITLFPEATVPAIASKTVKLIGTPAGKEAT
                     ETYVLHEDLTVEAAYGFLKTIEYIYTKGKEKEEINNTTLKFDKIADYRKALIQYLYQK
                     GGFKTADSFIKYLEKYAFETPVKVTKSYIESIMPAPVSNSEPKEAPQ"
BASE COUNT          979 a         1137 c         1019 g         1050 t
ORIGIN      
        1 cgcttcggtg agcagtttga aacgttccgc ctccgcgacc agcttgatgc tttcggcctg
       61 cccttcagcc tccaaaattt tggcttgccg gctcccctcc gccctcagga tttctgactg
      121 tttgatgccg tcggcttcga gaatggccgc gcgcttcgtc ctttccgcct tcatctggcg
      181 gctcatcgca tccataatgt cctgaggagg atcgatcttt tttatttcca cacgcgtcac
      241 ctttactccc cacttgtcag tggcgtcgtc caagacctcc cgcaatttcg cgttgatcat
      301 ctctcgagac gtgagcgtct ggtccagttc caattcgccg atcacgtttc ttaggttggt
      361 ttgggctagc ttgatcgccg cgacctgaaa gaaggccacg ttgtaaatga cttgataggc
      421 gtcggtgatc atgtagtaga taaccgcgtc gaccgcgacg acgacgttat ctttcgtgat
      481 gacttcttgt ggcggcacat cgataacctg ttctcgcata tccaccatcg tcatacgctc
      541 gaaaaacgga ataataaagt ggatgcccga atccgctatc cggttgtact tccccaaccg
      601 ttccactaaa cccttttgga atggtctgac gatccgaatg ccggtgccgg cgaaaaccat
      661 aaccagtatc gcaattgcaa taattccgat caacatacga tacccctccc ttttatttta
      721 tttcgaccgg gtccacggta accgtgaccc ccttcagttc gatcactttg atcttggcgc
      781 cgacgggaat ctcttgctca ctacgcgacc gtgcccgcca tatctcttta tcgatacgaa
      841 cgaagccttt gccgccgtct gcggtgatcg tttcggtgac tacccctatt cggccgatca
      901 tctcatcttg aacgatcagg cgggagggtt tgccgctcat tttttgcgcc aatttgcgcg
      961 taaacagaac gaggatcaaa gaaacgagga taaacgcgat cgattggtat agaacgctgt
     1021 tctcaaacaa aaacgcgacc agcatcgcgg cgaaggcgcc gatgccaaac cacataatga
     1081 aaaatgaagg tgtgaatatt tccgcaatga ggaacagaac gcctaatatc agccaaaata
     1141 tcgtggtttt cattcaaatc gcctcttttc ttctatcgat ccactgattc cattcgcatt
     1201 cacgatagaa ataaacttta aaagttgtaa cacaacctta aacttctttc gagggctctg
     1261 cagaatccgg tagagccact ccatattcat acgctgaatc cacccgggcg ctcgtttcac
     1321 gacaccggcc catacatcga gagaaccgcc gacaccgatg cccagacgcg cgttcgtctt
     1381 ttcgaaaaat cgttcgagcc atatctcttg tttcggggcc cctaaagcta cgaataagat
     1441 cgatgccccg ctttcggata tctcctggca gatacccagt tgcgtgtcat cgtcgggatt
     1501 gtggaacccg tgcctatatc cggctatccg cacccctgtc tcctgcgtca aaaaagtggc
     1561 cgccttctcc gcgactcccg gttttgagcc gaacagaaag acgctttcac cacttttccc
     1621 cgcgagacgg cagagttcaa agccgagttc gaccccggga aatcggctga cccgcgtatt
     1681 caatcgcttc cgtatcgccc aagacagccc gacgccatcc ggaatcacga gggtgccttt
     1741 caaagccacc tggtattccg gcatcgtcaa gtagtagtag gcgatctgcg cgttcatcgt
     1801 ggtcacccga aaggttctgc ctacgtctag ggcccttcgc aaggccgtca aaacggcctc
     1861 cctatcactc ttcactatcg gcagtccata aaagttcaca aaagaaagac attccatgcg
     1921 aacccctctt tctctacata agacgaaaaa agccatcgaa cgtacggaca atctaatcga
     1981 atatctatgg agaatacggc aagattatac cacgtttttc gcggatacac acgggctgtg
     2041 ttttccggaa gaggctggga acgtgggaac gccgaacttc agttcggcat gtttttattt
     2101 ttctgggagc tttccgagga gcgccagctt ttaaattggc cggccttaag aacaaaacag
     2161 gggttcgcac agagaacacg gagtacacag agagaacaca gagaaaagcc ctgggaacgc
     2221 cgaactttag ttcggcatgt attttctttt tttgggagaa cgttgtccat cgccgatctt
     2281 tcctctgtga tctctgtgcc ctctgtgcga accctgtttt tcaagacagg tacaaaaacg
     2341 tatcgcgcca tagggtaatc gcggggtaac cgaagggacc ccgtgatccc cctgtaaaaa
     2401 agcgtatcgc gccgtcgcga aaagaagggg cgcttacgcc ccgatgactt cctaaaacat
     2461 gccaatttaa aagttgccgt tcccagggcc ttcccagaga gacaggccga ctggaagtcg
     2521 gccctacgtg ctttcttcag tcttttctct gtgttctctg tgccctctgt gcgaaccctg
     2581 ttttttgatc tttgaaacga acgaaacccg gggtatcgcg ccgtcgcaaa aagaaggaac
     2641 gcttacgccg cgatgccctc acacaaaagc gtatcgcgcg gtcgcgaaaa gaaggggcgc
     2701 ttacgccgcg atgacttccc aaaaacttaa accttgccga gctcacttaa accatttgta
     2761 gaagaggagg ccgaaccatt cgtgtagggc ggcggcggtg gcggtgaagg cgccgcttga
     2821 agggaggaag tccagccata tggtctctcg ttcttcaata tagaagtcag tcggtatcgg
     2881 taccaaccag agcggcgaag agcgcttttt tgcttccctt tcgaaggcga gcatcgctcg
     2941 aggcaggtga atcgcgctcg tgacgatata caccgtcgct actgcctcat caaggagggc
     3001 gagtgagtag gcggcgtttt gatcggtggt catcgcgcgg tcttcgatga cgatgtcttt
     3061 ctccggaact ccccacatcg ctagcgtttc ggccatcgtc tctgcaacgt gcacttgatg
     3121 agagctccag agtgggcccc cgctcacgat gatcggcatc ggccgttcac gatagagaga
     3181 gaaccctctg ttgagccgga gggccgtcgt tctgtctggc tcattcgtaa cctcgccgcc
     3241 gtacccggac ggtacgccca cgcgatggct gctgctgagg atgacgatcg ccgattggga
     3301 agcgtcgacg ccaacctctt gcgcatagga agtggcaaaa tggttttcta gaggggcgac
     3361 gagccattgg ctaaagaagc ccgcggaaca gaggtagaac acaacgatca atacggatag
     3421 aacccttcgc caaccgcggc tcattttcgc gaacagcgtg aaagcgagga gaatcagtat
     3481 caaaagcccc ggtaattcca caaatgcgct caatgttttt tgtagtataa gcaacgccat
     3541 ccactctttc gcgtttgagt tattgattcg cgggaccaaa tcctcggata aagaaagacc
     3601 gcgcccgtac cagtggggac ggttcggctc gggcacgatc ggtacagatg ttatcaaata
     3661 caccgcgctt cggtattcat tcagctcaga aacgagaaaa gcggtttcga atctctacga
     3721 gacgacgcgt gttctactgc ggtgcttctt tgggttcgct gttcgaaacg ggcgcgggca
     3781 taattgactc gatgtaagac ttagtcactt taaccggtgt ttcgaatgcg tatttctcta
     3841 aatatttaat aaaactatcg gctgttttga atccaccctt ctgatacaag tactggatca
     3901 aggctttacg gtagtcagcg attttatcga atttcaaggt tgtattattt atctcttctt
     3961 tctccttacc ttttgtatat atatactcga tcgttttcag gaacccgtag gcggcttcaa
     4021 cggttaggtc ttcgtgaagc acgtatgtct cggtcgcttc ttttcctgcc ggggttccga
     4081 tgagtttgac tgtcttactg gcgatagctg gaactgtagc ttctgggaat agagttataa
     4141 aagcgttata agatttttcg agttctaaga ctccgttttc cgtat
//
LOCUS       DAOPXN010000199         4184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_21427_length_4184_cov_4.207798, whole genome shotgun sequence.
ACCESSION   DAOPXN010000199 DAOPXN010000000
VERSION     DAOPXN010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_4184_cov_4.207798"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..918
                     /locus_tag="PKM49_09050"
     CDS             226..918
                     /locus_tag="PKM49_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY82980.1"
                     /translation="MVKVVNLRKSYRTGSTYFEALKGLNFTIQRGEILSILGPSGCGK
                     TTLLNCLSSIDHPSEGKVYIEEVDLHALSDNEKTLFRAKNMGFIFQFYNLIPVLTAAE
                     NVELPLFAIDSNRKRVRNAAEEVLTRLGLKDRLNYYPSDLSGGERQRVSIARALVYRP
                     KIIWADEPTGALDTQTGREIMDVILELNEKNGQTFVIVTHDERIADYSNRIVSMDSGQ
                     IIRIRELSPVRA"
     gene            920..3961
                     /locus_tag="PKM49_09055"
     CDS             920..3961
                     /locus_tag="PKM49_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNY82981.1"
                     /translation="MILEILLVILSAVILLVLLLMAKNGIIASMSLRNIFRQPTNTFL
                     VILGSLMGTALITGSFAMTDSFNKFIYAQIEGEYGEIDEVLYKAEGSARQGGTYFSLG
                     EVSHWLEVLRRENLVDGILPVIRIEAQVKSLKENSQKAGLLQNYLKVSATGVDWSAIP
                     SFGLSSVAVPEPEETEGVAGVYISKDLASMLKVEPGDTLSFSTNILQQLAFWTRIPTV
                     RIAGILPEDSFLNYKGMGHGGVNGSVVMPESQLRRLINLSGEYETNAIWISNRGDFVT
                     GVRRIEAVAASFIAVDLPPAFKLDTTKKELVEVADEGNFGLIFLGLSAFAILAGILLI
                     SNTFKMLSEERQVELATMRALGYKRSRVTLVMVFEGFFYSVLSAVIGVVVGMLITSFI
                     LGQFAGFIHNVASVLPFELPIPMTQENITFGFYIKPISIFYSFILGISIPLVVIFYTS
                     FRNAKMNIVDSIRGLPPAWDQTSRKRFQVLWVVMAVVCVLVSAQGWRIRNELVFSIGI
                     FGASFFLPLCLPVKDKRWPVTLACFWVIGLSMLYRPSYALHELPNPWVILVKGVAILF
                     SCLLLVIFNLKVFESLLKSVFSHSRLSKGVTKIAIAFPSKNRTRTGLTISMYTLVVFI
                     VTLISIIPYSEEKVIRKSRDSFFIGNDLLIFRSPFGGNTGQSAPSLTEILATRPELSS
                     VSVLRSVRVQKVVTEEASSSLTFSLYAFNKGFSVSDAVSWTVIPSLKDEIVSPGDLIA
                     YLIAHPGTGVQSGFGYFEPGEEASLVGSTGSISRGGFADMQQMMTARKTAASTDQFPI
                     RVIGTLNENPISMVQGVLSYEKHVPDSFLKGAQTTTFASVKGATAGEKSAAVKSLSDE
                     LLSSGYLPLYADDIIKVISAMIEGIIGILRSFLYFGMVVGIAGIAILMFRALYERKRI
                     IGMLKAIGYTKTHIFQSFFIETSFIVLIGIVLGVVAGILTSQQFLSVLNFSELEIPLS
                     IPWGLLFTVCGVFYAVSLAVTFFPSYMAARIPPAEALRFFE"
BASE COUNT          901 a         1098 c         1134 g         1051 t
ORIGIN      
        1 gcgttcccag ggcgctcccg gggcggcgta aaaagtacag aaggcggatc gcggggtcgt
       61 aaaaaccact taccccgcga taatccacaa gaagtcagcc ccctggttac ccccgcgatg
      121 agtctctttg ttatgtcacc ccatatcttt gcccacacat aagcgttcac tcgtctgttt
      181 tggtatataa taaaaggatt ttaagagaag aggtgtgttg tggaaatcgt taaagtagta
      241 aatttgcgca aatcctatcg aacgggtagc acctatttcg aagcgctgaa aggcttgaat
      301 tttaccatac agaggggaga gatcctctcg atattgggcc cctcgggctg tggaaagacc
      361 actctgctga actgtctctc ttcgatcgac cacccgtcgg aggggaaggt ctatatcgaa
      421 gaggtggacc ttcacgcgct ctcggacaac gagaagaccc tttttcgagc gaagaatatg
      481 gggttcatct tccagtttta caacctgata ccggtcttga ccgccgccga aaacgtggag
      541 ttgccccttt ttgccatcga tagcaacaga aagagggtgc gaaatgcggc ggaggaagtt
      601 ttaactcgtc tcggcctcaa agaccggctg aactattatc cctcggactt aagcggcggc
      661 gagcggcaga gggtctccat cgcgcgggcg ctggtctatc gacccaagat catctgggcc
      721 gatgagccga cgggggcgct ggatacccaa accggtcggg aaatcatgga cgtcatcctg
      781 gagttgaacg aaaagaacgg ccaaaccttc gtcatcgtga cccacgacga acgcatcgcg
      841 gactattcta accggatcgt ctctatggat agcggacaga tcatccggat tcgcgaactc
      901 tctccggtga gggcttagcg tgatcttaga gatcctgctc gtcatcctct ctgcagtgat
      961 cctcctcgtt ctcctgctca tggcaaaaaa cgggatcatc gcttcgatgt ctctgaggaa
     1021 catcttcaga caaccgacga acaccttcct cgtgatcttg ggctcgctga tgggaacggc
     1081 cttgatcacc ggttccttcg cgatgaccga ttctttcaac aagtttatct acgcgcagat
     1141 agagggagaa tacggggaaa tcgacgaggt cctttacaaa gccgagggct ccgcgcgcca
     1201 aggcggaacg tatttttctc taggcgaggt ctcccattgg ttagaggttt tacgtcgaga
     1261 gaacctcgtg gacggtatcc tgccggtgat acggatcgag gctcaagtga agtcgctgaa
     1321 agagaactct cagaaggcgg gcctactcca gaattacctg aaagtcagcg cgacgggtgt
     1381 ggattggtcg gcgatccctt ccttcggcct ctcctccgtg gctgtaccag aaccggagga
     1441 aacagaaggg gttgcgggcg tttacatctc caaagacctg gcttcgatgc tgaaggtgga
     1501 accgggggac accttgtctt tcagcacgaa cattctacaa cagcttgcat tttggacgcg
     1561 catcccaacg gtgcggatcg cagggattct tccggaggac tcttttttga actacaaggg
     1621 gatgggccac ggcggagtga atgggagtgt ggtgatgccc gaatcgcaat tgcgacggct
     1681 gatcaaccta tccggtgagt atgaaaccaa tgcgatatgg atttctaacc gaggggattt
     1741 tgtgaccgga gtccgccgca tcgaggccgt ggcggcatca tttatcgcag tcgatcttcc
     1801 tccggctttc aaactcgata ccaccaagaa agagcttgtg gaggtcgcgg acgaaggcaa
     1861 ttttggcctg atcttcctcg gcttaagcgc tttcgcgatt ttggccggaa tcctgttgat
     1921 ctccaacacc ttcaaaatgc tctcggaaga acggcaagtc gagttggcga cgatgcgcgc
     1981 gctgggatat aagcgatcac gggttacctt ggtcatggtt tttgaaggct ttttttattc
     2041 ggtgctctcg gcggtcatcg gcgtcgtagt gggtatgctg atcaccagct tcatcttggg
     2101 gcagtttgcc ggcttcatcc acaacgtggc ttcggtccta cccttcgagt tgccgattcc
     2161 tatgacccaa gagaatatca ccttcggttt ttatatcaaa ccgatttcta ttttttacag
     2221 ctttatcctg ggtatttcga ttcctttggt cgtcatcttt tacacctctt tccggaacgc
     2281 gaagatgaac atcgtcgatt cgatccgggg cttgcccccg gcctgggacc aaacgtctcg
     2341 aaaacgattt caagtcctgt gggtcgtgat ggcggtcgtc tgtgtccttg tttcagcaca
     2401 gggttggcgg attcgaaacg aactcgtttt cagcatcggt atctttggcg cttccttctt
     2461 tttacccctc tgtctacccg taaaggataa acggtggccg gtgacccttg cctgcttctg
     2521 ggtcatcgga ttgtcgatgc tttatcgccc ttcttacgcg ctacacgagc tccccaatcc
     2581 ctgggtcatc ttggtgaagg gtgtggcgat cctgttctct tgtctcttgt tggtcatctt
     2641 caatctgaag gtcttcgaaa gccttttgaa atcagtgttt tcacatagcc gcctatccaa
     2701 aggcgtcacg aagatcgcga tcgcctttcc atcgaagaac cgcacgcgaa ccggcctcac
     2761 gatttcgatg tacacgctcg tcgtgttcat cgtgacgttg atctcgatca tcccctattc
     2821 cgaagaaaag gtcatacgga aaagtcgaga cagcttcttt attggcaacg acctgttgat
     2881 cttccgttct ccctttggtg ggaacaccgg tcagagcgcc ccttctctca cggagatcct
     2941 agctacgagg ccggagttat cgagtgtgtc cgtgcttcgc agcgtcaggg tgcaaaaggt
     3001 ggtcactgaa gaagcatcgt cgtctctcac tttctccttg tacgcgttca acaaaggttt
     3061 ttccgtaagc gacgcggttt cgtggacggt cattccctcg ctgaaggacg agatcgtctc
     3121 ccctggcgac ttgatcgcct atctgattgc gcaccctgga acaggggttc aaagcgggtt
     3181 tggctacttc gaaccgggag aggaggcttc gttggttggt tcaaccggtt ccatctctcg
     3241 gggagggttt gcggatatgc agcaaatgat gactgcgcga aagaccgccg cctcgaccga
     3301 ccagttcccg ataagggtca tcggtacgct caacgaaaac cccatctcta tggtgcaggg
     3361 ggtcttgtcg tatgagaagc acgtgccgga ctctttctta aaaggcgcgc aaacgactac
     3421 cttcgcctca gtgaaggggg cgaccgcagg agaaaagagc gcggcagtga aaagcctatc
     3481 ggacgagctg ctctcctcgg gatatttacc actttacgcc gacgatatca tcaaagtgat
     3541 ttcggcgatg atcgagggca tcatcggcat cttgcgatcc ttcctctact tcgggatggt
     3601 tgtcgggatc gccgggatcg cgatactgat gtttagggcg ttatacgagc gaaagcggat
     3661 tattgggatg ctcaaagcga tcgggtacac gaaaacgcat atcttccaat cctttttcat
     3721 cgaaacgagc tttatcgtgc tcatcggcat tgtgttggga gtggtcgccg gaattctgac
     3781 gtcgcagcag ttcctgtccg ttctgaactt ttcggagttg gaaatccccc tatccattcc
     3841 ttggggtttg ctgtttacag tctgcggtgt cttctatgcc gtctcgctgg ccgtcacctt
     3901 tttccccagt tatatggcgg cgaggatccc gccggcggaa gcgctccggt ttttcgaatg
     3961 aaaaaacctg cgcccgcccc gaactcgggc tcgacaagtc ttggtgtggg tcaagttatg
     4021 tggcgacata actcagaggt taatcgcggg gtaaccagcg ggctgacttc ggaagctcac
     4081 gaagtctcgc gatccctttt tcgtccttct aaaaagcgta tcgcgccgtc acgaaaagaa
     4141 aaagcgctta cgccgcgatg ctgcgtacgg gaacgccctg ggaa
//
LOCUS       DAOPXN010000200         4131 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_21779_length_4131_cov_5.767419, whole genome shotgun sequence.
ACCESSION   DAOPXN010000200 DAOPXN010000000
VERSION     DAOPXN010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21779_length_4131_cov_5.767419"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="PKM49_09060"
     CDS             <1..1107
                     /locus_tag="PKM49_09060"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HNY82982.1"
                     /translation="SSDTLPLLAFQSVDCPSIGPEPPPLKQITFEALGWVDQTVEVVY
                     TAEQTLGFSADAFGGIPQSAVLELEVSAAHFEEEETVTLAVFLNDRLVGSMALDIETP
                     VKTAFIPLPAEDFHGYNHLRFEAKKYRGECKSVGLEIQSDSLIRWESVEPMVQARFYD
                     FPYLLYGKTGIVVSTVSKETASILSTIMMKKGEGSLRTTAPELFTPNEVKEKSPPFQR
                     CDSFFFLIDETEVDRFFPEIRIHPGLRLIHPKTGSVMFAPDATASYVLLFVGQYEGKP
                     ALLGAVHGAPTLFENITETQWRRLKQAPGNLALLTEDGQVVSFTVGDALSVGDPALTV
                     ERKLDLRRILWILVIVSALIIFLISSYRKTSSNR"
     gene            1154..3016
                     /locus_tag="PKM49_09065"
     CDS             1154..3016
                     /locus_tag="PKM49_09065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNY82983.1"
                     /translation="MDKRLGAVLIEMGIITPSQLEEALTIQKQKGGRLGWLLATMGYV
                     SRNDLFRALSAHYGLRFATPELSHLLETVDIELAQRTPKEEALRLQALPYRIENRTLI
                     LLNAYPGNEQTALFYRTLYQVDRVEEWVVTDLDIFNLAKKVYGDPLLKEAVYGLFFRN
                     PEESAYRVFTFRQFSFILASLILLGIITALYPFETLRASLFVIQTLYVILIGFKFVLS
                     LFGTVDEITYREKPDEYQNLEDSDLPVYTVLVPVFREPEVVGTLIEALKRLDYPANKL
                     DIILLLEEVDQETLAAAKAAAPPANWRFLIVPKSKPQTKPKACNYGLLFARGKYLVIF
                     DAEDVPHPDQLKKAVLAFEESDASTICFQAALNYFNKDQNLLTQLFTLEYSFWFDYML
                     PGLYNLNLPIPLGGTSNHFRVDALKKIGGWDPFNTTEDADLGIRAAAEKYKVGIIRST
                     TYEEATSRVWNWIRQRSRWIKGYMQTTLVYNRRPLKLIQAIGLKQWLSFQLFIGGTPI
                     LFLINPILWILFGVWIGWNPPWLSELFTPALLFLSLFNFLIGNFLGIYLNLLGAFRRK
                     YYSLTFISLLNPFYWLLHSFSAYKALWQLLRKPHYWEKTHHGFAQPERRKEDAA"
     gene            3013..>4131
                     /locus_tag="PKM49_09070"
     CDS             3013..>4131
                     /locus_tag="PKM49_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82984.1"
                     /translation="MNRVTRLPVLFRSRTFWALFLLLFALYWFLNLRFQSAALYSPHQ
                     QWIRFHAKWLMRVSLEGMRAVDFEIPPLNLLLHILLVSDAHRIFLSALAGFLVAFLIL
                     YGLANVRTKTVKLLWSLYLALSPALWFSCLFLPDYVLYLILYCLAFYFLLEFSVSEQV
                     FYLFVFGLLFGLMGLIRFETLWMSIVVFVYFLLRYGKRGLFLYYALAALFPLVSLQLS
                     WVFLSLIFKGEAATLLRHLYVDPFFAFAAFSNPIEQFMLHMTWPFFFLYAVTIVRLGT
                     YREFFRSALFFSLLIPFLSPFFLKMTVQELCPVTFASLFIIHYLILFPYIGSLFNEKR
                     QKLFYSAFLVGYFLFTLFAFSVTPEEHEALFFKRLGDAN"
BASE COUNT          831 a         1121 c         1047 g         1132 t
ORIGIN      
        1 tcctccgata ccttgccgct tctggctttt caatctgtcg attgtccatc tatcggcccc
       61 gaacctcctc ccttaaagca gatcacattc gaagcattgg gatgggtgga ccaaaccgtc
      121 gaagtggtct atacggccga acagacgttg gggttctcgg cggacgcgtt cggtggcatt
      181 ccgcaatccg cggttctcga gctcgaggta tccgcggccc atttcgagga agaggagacg
      241 gtgaccctcg ccgtctttct caacgatcgg ttagtcggat cgatggcgct tgatatagaa
      301 actcccgtga aaaccgcctt catccccttg ccggcggaag atttccacgg ctacaatcac
      361 cttcgatttg aggctaaaaa atatagaggg gagtgtaaaa gcgtcggttt ggagatacaa
      421 tccgactccc ttatacgctg ggagtccgtg gagcctatgg tccaagcgcg gttttatgac
      481 tttccatact tgctttacgg taagaccggg atcgtagtct ctacggtttc gaaggagacg
      541 gcgtctatcc tttcgacgat tatgatgaag aagggagagg gttcgctccg aacgaccgcc
      601 cccgagctgt tcacgcccaa cgaggtcaaa gagaaatctc cgcctttcca acgatgcgac
      661 tccttcttct tcctcataga cgagacggag gtcgaccgct tcttccctga aattcggatt
      721 caccctgggt tgcggctcat acatccgaag acgggatcag tgatgttcgc tccggacgca
      781 accgcctctt atgttttgtt gtttgtgggc caatacgaag gcaagccggc gcttctaggc
      841 gccgttcacg gggcgcctac gttgtttgaa aacatcacgg aaacacaatg gcgccggcta
      901 aaacaggcgc ccggcaactt ggcgctgttg actgaagacg ggcaagtggt cagttttacc
      961 gtcggcgacg ccttatcggt aggggatccg gcgctcacgg tcgagcggaa attggatctc
     1021 aggcgtattc tttggatcct ggtcatcgtc tccgctctca tcattttctt gatctcctct
     1081 tatcgtaaaa cctcttccaa ccgatgacga gtgtagagaa cgccgaaaag cgggttcttc
     1141 gcggagtgtg cttgtggaca aacgattagg cgccgtattg atagagatgg ggatcatcac
     1201 gccatcacag ctggaagaag cgttgacgat tcaaaaacag aaaggcggtc gcttgggctg
     1261 gctgttggcg actatggggt atgtcagtcg gaacgatctg tttcgggcgt tgagcgcgca
     1321 ttatggcctg cgtttcgcca cccccgaact ctcccatctt ttggaaacgg tggatatcga
     1381 gctcgcccaa aggaccccga aagaagaggc cctacggttg caagcccttc cctatcggat
     1441 agagaatcgg acgctgatcc ttctcaacgc ctacccgggt aatgaacaga ccgctctttt
     1501 ctatagaacg ctttatcaag tcgatcgagt agaggaatgg gtcgttacgg acctggatat
     1561 tttcaacctg gccaaaaagg tatacggaga ccctttactg aaagaggcgg tttacggtct
     1621 gttttttcga aatcctgaag aatccgctta cagagttttc acctttagac agttttcttt
     1681 catcctcgcg tccctcatcc tgctgggaat tatcactgcc ctttacccct tcgagacgct
     1741 tcgggcgtcg ctcttcgtga ttcaaacgct gtacgtgatc ctgatcggat tcaagttcgt
     1801 gctcagcctg tttggaacgg tggatgagat cacctatcga gagaagcccg atgaatatca
     1861 gaatttggag gacagcgatt tacccgttta cacggtttta gtccctgttt ttagggaacc
     1921 ggaggtggtc ggaaccttga tcgaggcgtt gaagagactg gactaccccg ccaacaaact
     1981 cgacatcatc ctattgttgg aggaggtgga ccaagaaacc ctcgctgcgg cgaaggcagc
     2041 cgcgcctccc gccaactggc ggtttttgat cgtaccgaag tccaaaccgc aaaccaagcc
     2101 caaagcctgc aactacggcc tgctgttcgc ccgcggcaag tacttggtca tcttcgacgc
     2161 cgaagacgtt cctcatccgg accagctgaa gaaggcggtg ctggcctttg aagagtccga
     2221 cgcgtcgacg atctgtttcc aagccgcgct gaactacttc aacaaggacc agaacctcct
     2281 gacccagttg tttacgttgg aatactcgtt ctggttcgac tatatgttgc cgggcctata
     2341 caacctcaac ctgccgattc ccctcggcgg gacgagcaac cacttccggg tggacgcgct
     2401 gaaaaaaatc ggtgggtggg acccgttcaa taccaccgaa gacgctgatc tgggaattcg
     2461 cgccgccgcg gagaagtaca aagtggggat catccggtca accacctacg aagaggcgac
     2521 ctcgcgtgtc tggaattgga tacgacaacg ttcccgttgg atcaaggggt atatgcaaac
     2581 gactctcgtc tataatcgac gtccgttaaa gctgatacag gcgatcggac taaaacagtg
     2641 gttgtctttc cagctgttca tcggaggcac tccgattctc tttctgatca atccgatcct
     2701 ctggattctt ttcggcgtct ggatcggttg gaatccaccg tggctctccg agctcttcac
     2761 accggccctg ttgttcctgt cgctcttcaa tttcctgatc ggcaactttc taggcatcta
     2821 tctcaatctc ctgggggcct tccggcggaa gtactattcg ttgacgttca tcagtttgtt
     2881 gaatcccttc tattggctgt tacattcctt ttccgcctat aaagcgcttt ggcaattgct
     2941 acgcaaaccc cattactggg aaaagaccca ccacggattc gcgcaaccgg agcggcggaa
     3001 ggaagacgcg gcgtgaatcg ggtcacgcgg ttgccggttt tgttccgatc gagaaccttc
     3061 tgggcgctct ttttgttgct atttgcccta tactggtttc tgaacctgcg ctttcaatcg
     3121 gccgcgctgt attctcccca ccagcaatgg attcggtttc acgcgaaatg gttgatgcgc
     3181 gtttctttag agggaatgcg tgcggttgac ttcgaaatcc cgccgctgaa cttgcttttg
     3241 catatcttgc tcgtgtcgga tgcccaccgc atcttccttt ccgcgctcgc cggttttctc
     3301 gtcgcgtttt tgatcctgta tggcttggcc aatgtccgca cgaagaccgt caagttgttg
     3361 tggagcctgt atttggcgct ttctcccgcg ctgtggtttt cctgcctgtt tttacccgat
     3421 tacgtcttgt acctgattct gtattgcttg gccttctatt tcttgttaga gttttcggta
     3481 agcgaacagg ttttctatct cttcgttttt ggattgctgt tcggattaat gggattgatc
     3541 cgctttgaaa ccctatggat gtcgatcgtg gtcttcgtct atttcctgct gcgctatgga
     3601 aaacgagggc tgttcctcta ttatgccttg gccgctctat ttccgttagt ctccttgcag
     3661 ctttcctggg ttttcttatc cctgatcttc aaaggggagg ccgccacgct cctgcgccat
     3721 ttgtacgtcg atcctttttt tgcctttgcc gccttttcga accctatcga gcagtttatg
     3781 ttgcatatga cttggccttt ctttttctta tacgctgtga ccatcgttcg gctcgggacc
     3841 tatcgcgagt ttttccgttc cgcgctcttc ttttccctac tgatcccatt tttatctcct
     3901 ttcttcctga aaatgacggt gcaggagctg tgcccggtca ccttcgcgtc gcttttcatc
     3961 atccactacc tgatcctgtt cccttacatc ggcagcttgt tcaatgaaaa gcgtcaaaag
     4021 ctcttctaca gtgcgttttt ggtggggtac ttcctattca ccctcttcgc gttctcggta
     4081 acgccggaag agcacgaggc tctcttcttt aagcgacttg gagatgcgaa c
//
LOCUS       DAOPXN010000201         4098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_21996_length_4098_cov_6.640613, whole genome shotgun sequence.
ACCESSION   DAOPXN010000201 DAOPXN010000000
VERSION     DAOPXN010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21996_length_4098_cov_6.640613"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            355..2463
                     /locus_tag="PKM49_09075"
     CDS             355..2463
                     /locus_tag="PKM49_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY82985.1"
                     /translation="MKKGLFFSFCLALSLLFLSLSLSSCFLKGHAPQKILLFLHSTPY
                     DGISVKVNGAPYETNTQCAVQANNPVILQVDQSKAFDLSGRVGGDDTRLIFERWSSGE
                     TDPIIGLIPQKNATYTAHFGVEYYVSFESSATSARGASDPSGWYASGTQLQLEAPSIA
                     GKDFLRWTVNGESAGNAKTLSLLVDSPKAVVAVYEMMKQPPSSFTTLSPTNGATQVNH
                     EPYIQFLWNASVDPQDSSVYYNLYLGESAQTMEIVATGIAGTFYHHDRCVGSKTYYWK
                     VEAVNAYGLKTSTGVMRFSTGAFPPSVPQPVFPRDGAVDVPYSFTLEWECTGGAHYDL
                     YYWITESASTLVQNIRETRYTLNYLEQGVTYHWKVIAYNALGSARSSVWQFTVTESAI
                     PPSNAHSPSPRNSAIDQELTVTLSWGQPVVGAKPFTYDVYFGPAPTYDIEPRAMRRVL
                     SDSPNTSVTVEGLDPGTHYIWQVISKNPWGVSASVIWTFMTQIGVPKPSEPSNPTPAT
                     GATEVSTNVTMSWNPSVNGAITYDLYLGTATNTLDKVCSSLSNPSYQPANLALDTTYF
                     WKVIARNQSGFQESEVWSFTTATERAGVSLEKVNETTFLLRANRYFPLESAVRFALDG
                     PYEGCTALTISPIQSSSGCLGEGKIFNFADARSSFSHFSPSNLSIATVTCNAPGTHEL
                     VYFYNGSDAIPILNATVSLP"
     gene            2972..>4098
                     /locus_tag="PKM49_09080"
     CDS             2972..>4098
                     /locus_tag="PKM49_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HNY82986.1"
                     /translation="MRTFCTEGPVDPKLYYYVPRTALIAEGLKKVEQWRYFTIFAPRQ
                     SGKTTYFQFLIEAIKREKPDWLPVWVSFEAYGDIELSYFLSSLRDRILRGTELPEETV
                     LDFYNMDDIALKLREVARFQKKEWVIIVDEIEGLKNLKVLNGFLHLIRSIYHEKQAIG
                     LRSVILTGVSNITGIIQDTASPFNIADQIQVPYFTREEVYDLLAQHERETGQLFENPV
                     KEGIYEQTLGQPGLVNALARDLVEKECPNGENIELRSLYTTLDDFTEYYIDKNIANII
                     AKAKLYPKTVEEILFEEPLPFKITQEDIGVLYANGVIDRDPEGNCYIPVPIYKKALYH
                     HFKPKTNGERRHFKSPIETYKKYLDERGLLDVTKLLWRYIA"
BASE COUNT          968 a         1110 c         1036 g          984 t
ORIGIN      
        1 ggcgctccca gagcgcttcc agataatttc taatctctcc tctgtgttct ccgtgttctc
       61 tgtgcgaacc ctgtttttca ggacttgtgc aaaagcgtat cgcgccgtcg cgagaaaaag
      121 aagaagcgct tacgccgcga tgctgtgctt cgaaacgttc ccagcagtgt tcccagggac
      181 ggcctagaaa tatcggggtt gcattctccc tttttttccg ctataatatt ctatcgggga
      241 gatttttcct ttccattttt cgtctaatag atttgggtgt tctttttctt tttgtgattc
      301 ggtatcacgg ttttttattc gatatgtttt tatttgagtc aggggggtca ttctatgaaa
      361 aaaggacttt ttttctcttt ttgcttggcg ctttcgctac tcttcttgtc gcttagcttg
      421 agttcttgct ttcttaaggg gcacgcccca caaaaaatac tcctcttttt acacagcact
      481 ccgtatgacg gcatctccgt taaagtgaac ggcgcgcctt atgagacgaa cacgcagtgc
      541 gccgttcaag cgaacaatcc tgtgatttta caggtcgatc agtcgaaagc tttcgatctg
      601 agcgggcgag tcggcggtga tgatacgcgt ctgatctttg agcgttggtc ttccggtgaa
      661 accgatccga tcatcggcct aatcccccag aagaatgcca cctatacggc acatttcggg
      721 gtggagtact atgtgagctt tgaaagtagc gccacatctg cgcggggggc ttctgacccg
      781 tccggttggt acgcatccgg cacacagctg cagttggagg ctccgtctat cgccggcaaa
      841 gattttttac gctggacagt gaacggggag agcgcgggga acgcgaaaac tctttcgttg
      901 ctcgtcgata gcccgaaagc cgtggtggcc gtctatgaga tgatgaaaca acctcccagc
      961 agttttacca ctctttctcc gaccaacggc gccacgcagg tgaaccacga accgtacatc
     1021 cagttcctct ggaacgccag tgtggatcct caggatagct ccgtctatta caacctctac
     1081 ctcggagaaa gtgcccagac gatggaaatc gtcgcgaccg ggatcgccgg aaccttctat
     1141 caccacgatc gttgtgtcgg ctcgaagacc tattattgga aggtcgaagc agttaacgcg
     1201 tatggcttga aaacgtctac cggggtgatg cgtttttcca caggcgcgtt cccgccgtca
     1261 gtaccccaac cggttttccc aagagatggc gcggtcgatg tgccctactc cttcacgttg
     1321 gaatgggaat gtacgggcgg cgcgcattat gacctctatt attggatcac cgagtcggcc
     1381 agcacgttgg tacaaaacat acgcgagacg cgatacacac tgaactatct ggagcaaggg
     1441 gtgacctatc attggaaggt catcgcctac aacgctctgg ggagcgctcg gagcagcgta
     1501 tggcaattca cggtcacgga gtctgccata ccaccgtcca acgcgcattc tccgtcgccg
     1561 cgaaactccg ccatcgatca ggaattgact gttactcttt cttggggtca gcctgtggtt
     1621 ggagccaagc ctttcaccta tgacgtctat ttcggaccgg ctccaacata cgatattgaa
     1681 ccgagggcga tgcgtcgggt tctttcggat agcccgaaca cttcggtgac agtcgaaggt
     1741 ctggacccgg gcactcacta catttggcag gtgatctcga agaatccatg gggcgtttcc
     1801 gcgagtgtga tttggacctt tatgacgcag atcggggtgc ctaagccctc agaaccgtcc
     1861 aatcccacgc ccgctacggg cgcaacggag gtttccacca atgtgaccat gtcgtggaac
     1921 ccttccgtga acggcgccat cacttacgac ctctatctcg gaactgcgac gaataccctc
     1981 gacaaagtct gcagctcgct ctctaacccc agctaccagc cggcaaacct cgcgctcgac
     2041 acgacctatt tttggaaggt gatcgcccga aatcaatcgg gtttccaaga aagtgaggtt
     2101 tggtccttca cgacggcaac agaacgcgcc ggcgtttctt tggagaaggt caacgaaacc
     2161 accttcctcc tacgggcgaa tcggtacttt cctcttgaga gcgcagtgcg attcgctctc
     2221 gatgggcctt acgaggggtg tacggcgctc accatctctc cgatacagtc ttcgtcagga
     2281 tgtcttggag aggggaaaat attcaatttt gcggacgcgc gcagctcttt ttctcatttt
     2341 tctccatcga atttatccat agcaaccgtt acgtgtaatg cccctggaac gcacgagttg
     2401 gtatattttt ataacggtag cgatgcgatc ccgattctta acgcgacggt ctcgctaccg
     2461 taataggcgg ctcttataga ccgacaacgc gccgctccct cccggcgcat ttttttgatg
     2521 cgtttctctg gagcgaaaaa agcgattaag gggtttcgta cggaggacac gcctgggtac
     2581 gccaactttt taagttggca ggttttaaaa gatagtctcg cacagagggc acggagggca
     2641 cggagtttgc tcgtaaacgc ccatacagat gagtaaaaat ccaaaaaaat aaccaggccg
     2701 aactaaagtt cagcgttccc agtccgtgaa gtctcgcgat ccccactctg tccatcccaa
     2761 aaacgtatcg cgccctcgcg aacagaagaa tcgcttacgc cgcgatgctg cgcttggggg
     2821 cgccctcagt gaaaaccaaa aacatgccgg actaaagtcc ggcgctccca gagcgttccc
     2881 acggcgttcc cggagcgttt cggcagcgct ccgaaaaatc cggcgcttcg attgtggtat
     2941 actcgtttca aacccatcgt aggaggacca aatgcggacg ttctgcaccg aagggcccgt
     3001 ggacccgaaa ctgtattatt acgttccccg aacggcgctg atcgccgaag ggttgaagaa
     3061 ggtcgaacaa tggcggtatt tcaccatctt cgccccccgt cagagtggga agaccacgta
     3121 cttccaattc ctcatcgaag cgattaaaag agaaaaaccc gattggttac ccgtgtgggt
     3181 ttctttcgaa gcgtacgggg atattgaact ctcctatttt ctctcttcac tacgcgatcg
     3241 gattcttcgt gggacggagc tgccagaaga gaccgttcta gatttttaca atatggacga
     3301 tatcgccttg aaattgcgtg aagtcgctag gtttcaaaaa aaagagtggg ttattatcgt
     3361 cgacgaaatc gaagggctca aaaacctgaa ggtgttgaac ggttttcttc acctgatccg
     3421 ctcgatctat catgaaaaac aagcgatcgg gctacgcagc gtaattctga ccggagtgag
     3481 caacatcacg gggatcatcc aggacaccgc gagccccttc aatatcgccg accagatcca
     3541 agtcccctat ttcacccggg aggaagtcta cgaccttctt gcccaacacg aacgggagac
     3601 ggggcagctc ttcgaaaacc ccgtcaaaga gggcatctac gagcaaacct taggccagcc
     3661 gggcctggtg aacgccctcg ctcgtgacct cgtggagaag gagtgcccaa acggggaaaa
     3721 catagaactg cggtctttat atacgaccct cgacgatttc accgagtatt atatcgacaa
     3781 gaatatcgcc aatatcatcg ccaaagcgaa actgtatccc aaaaccgtcg aagagattct
     3841 ctttgaagaa ccccttcctt tcaagattac acaggaagat atcggtgtcc tctacgcgaa
     3901 cggcgtgatc gatcgcgacc ccgaaggaaa ttgctatatt cccgtgccta tctataagaa
     3961 ggccctgtac caccacttca aacccaaaac caacggagag cgaaggcact tcaaaagccc
     4021 gatcgagacg tacaagaagt atctggacga acggggactg ttggatgtga cgaagctctt
     4081 gtggcggtat atcgccta
//
LOCUS       DAOPXN010000202         4080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_22135_length_4080_cov_5.432795, whole genome shotgun sequence.
ACCESSION   DAOPXN010000202 DAOPXN010000000
VERSION     DAOPXN010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22135_length_4080_cov_5.432795"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="PKM49_09085"
     CDS             complement(<1..1030)
                     /locus_tag="PKM49_09085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY82987.1"
                     /translation="MSNTRKFMLHGFFSYLLLGLFSTGFSVAIPIIRAEFQLNYEAIG
                     IVLTMNSIGFLLGSLCGGISVEKLSLKITNTLGLVLAPVGLFLLITAKTLPVFGFGNF
                     LVGFGCSFLETGIPPLASFFSKKTARLLNLLHSFFSLGALASPVVFSLLTHFDLNWRF
                     LFGGMIVFFVLDLYLILTLKIEGEFRPSKIESKEGLRFFTSPFFWIIGIGVAFYVASE
                     LAISSWAPTYAVDIFEYAPTAAAFLPTFFWIGLFIGRLVSAAFVERFSPYRWLLLVTS
                     LGLPIVLLNQIPARPLFYFYGMTFLAGIVHSSIYPTFQALLVQHIDAGKGYALGVFAS
                     MGALGGFAS"
     gene            complement(1065..2120)
                     /locus_tag="PKM49_09090"
     CDS             complement(1065..2120)
                     /locus_tag="PKM49_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HNY82988.1"
                     /translation="MKKTPRIIQWFILSTILGLVVLGLIMNANGFDVVKSHIVRYPVP
                     YILSAIFVAFSRWLFEALSLVVALKPRRQISMWLAIKTSIVTHFFNLLTPFYSGGQPF
                     AIYYLTKHEIDAGVSTAAILMKSLIFQAMLTGLGLFGAVFCRGYLTSITLSALLLGSF
                     LNGSLFFIIFLFGMNRKLALWTVDGVLLLLCKIRLVKNRDKWRGKVLEKVHQFNDTFS
                     EYKQHPGHLLAITLCSIAQFFLHILSAAVILEGFKIHLSLPLFFQLSNMEITASVVPT
                     PGTSGGIEYIFILFLGQFGPIEKISAGLVLWRFCTYYFEISALGLLSVWIYRKIKVRH
                     PALSQEWPVKESVNRTP"
     gene            complement(2117..2722)
                     /locus_tag="PKM49_09095"
     CDS             complement(2117..2722)
                     /locus_tag="PKM49_09095"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HNY82989.1"
                     /translation="MMKERTTVYYETAGSDCTRKTLERCVERLLNWDLSTVESESPTL
                     IIPSTTGRSALLALDLLSAHPQIHLIVVGHRYGFREAGKNEIPDETQKKIRDRGYGLF
                     FGTHLFTGLEKAVSNAYGGAYPHRLIADTLRIFSPGTKVLFEDTVMATDAGLVAPNRW
                     VVAAGGTGRGLDTAMVIKSAHSDLFFQIRIREILCLPEGAL"
BASE COUNT         1081 a         1020 c         1071 g          908 t
ORIGIN      
        1 cagacgcgaa cccgcccaaa gcgcccatcg acgcgaacac acccaacgcg taccccttgc
       61 cggcgtcgat gtgttgaacg agaagcgcct gaaaagtggg atatatcgat gagtgaacga
      121 ttccagcgag gaaagtcatt ccgtaaaaat agaaaagcgg tcgcgcggga atctggttca
      181 agaggacgat tggcaacccg agcgaggtga ccaggagcaa ccaacggtaa gggctaaacc
      241 gttccacgaa ggcggcggag acaagccttc cgatgaacaa accgatccaa aagaaggtcg
      301 gcaggaaggc ggccgcggtg ggcgcgtact cgaagatgtc cactgcgtag gtaggcgccc
      361 atgagctgat tgctaattcc gaggcgacgt aaaatgcgac accgatcccg atgatccaga
      421 aaaagggaga ggtaaaaaac cgcaggccct cttttgactc gatcttcgaa ggccggaact
      481 cgccttctat ttttagggtt aggataaggt agagatccag gacgaaaaag acgatcatac
      541 ccccgaacag gaagcgccaa ttcaaatcga aatgcgtcaa gagggagaaa acgacgggtg
      601 aggccagcgc cccaagcgaa aagaacgagt gtagcaagtt gagcagccgg gcggtctttt
      661 tagaaaagaa ggaggcgagc ggcggtatcc ccgtttctaa gaaagaacag ccgaaaccga
      721 cgagaaagtt gccgaacccg aaaaccggga gcgtctttgc ggtgatcagc aaaaacaaac
      781 ctaccggggc gaggacgagt cccagcgtgt tagttatttt taatgaaagc ttctctacgc
      841 taatcccacc gcacaaagag cccagcaaga atccgataga gttcatcgtg aggacgatac
      901 caatcgcttc gtaattgagt tggaactctg cgcggatgat cggtatcgcc acggagaatc
      961 cggtggaaaa tagacccaga agaagataag aaaagaaacc atgaagcatg aactttcggg
     1021 tattactcac tggtcacctt ccgcgaataa tcgactattt actgttatgg agtgcgattc
     1081 acgctctctt tgaccggcca ctcttggctg agcgccgggt gtcggacttt tatcttccgg
     1141 taaatccaaa ccgataagag gcctaaagcc gagatttcga aatagtaggt gcaaaatcgc
     1201 caaaggacca atccggcgga aattttttcg ataggaccga actggcccaa aaacagaata
     1261 aagatgtatt ctatgccccc cgaagtcccg ggagtgggca cgacggacgc ggtaatctcc
     1321 atattcgata actggaaaaa caatggaagt gacagatgga ttttgaagcc ctcaaggatc
     1381 acggccgcgc tgagaatgtg caggaagaat tgggcgatag agcacaatgt gatcgccaaa
     1441 agatgccccg gatgctgctt atactctgag aaggtatcgt tgaattgatg gaccttctcg
     1501 agcaccttac ctctccactt atctcgattt ttcacgaggc gtatcttgca taaaagtaga
     1561 agaacgccgt ccaccgtcca aagggcgagt ttcctattca ttccgaacaa aaagatgatg
     1621 aagaacagcg atccgttgag aaaagacccg agcaacaagg cagagagagt gatactggtt
     1681 aagtacccgc ggcagaagac cgccccgaaa aggcccagcc cagtcaacat ggcttggaaa
     1741 atcagcgatt tcatcaggat agcggcggtg gacacgcccg catcgatctc gtgtttggtg
     1801 aggtaataga tggcgaacgg ttgcccaccg ctataaaaag gcgtcagtaa attaaaaaag
     1861 tgcgtgacga tcgacgtttt tatggccaac cacatagaaa tttgcctacg cggcttcaag
     1921 gccacaacca acgagagggc ttcgaatagc caccgtgaaa aggcgacgaa gatggcgctc
     1981 aggatatagg gaacggggta tcggacgata tgggatttga ccacatcgaa cccgttcgcg
     2041 ttcatgatca gcccgagcac caccaaccca aggatcgttg aaaggatgaa ccattggatg
     2101 atccgagggg tttttttcaa agcgccccct ccggcaagca caggatctcc cgtatacgta
     2161 tctggaagaa aagatcgctg tgagccgatt tgatcaccat ggcggtgtcc agccctctgc
     2221 cggttccccc agccgccaca acccaacggt ttggggcgac gagtcccgcg tccgtcgcca
     2281 tcacggtgtc ctcgaagagt accttcgtcc ccggagaaaa aatgcgcagc gtgtcagcga
     2341 tgagccgatg gggataggcg cctccgtagg cgttcgaaac ggccttttcc aacccggtga
     2401 ataggtgtgt cccaaagaac agtccgtacc ctctgtcgcg tatctttttt tgtgtctcgt
     2461 ctgggatctc gttctttccc gcttctctga atccgtagcg atgaccgacg acgatgaggt
     2521 ggatttgggg gtgggcggac agcaggtcta acgccagcag cgcggaacgc ccggtcgtcg
     2581 agggaatgat taaagtgggc gattccgatt ctacggttga aaggtcccaa ttcaaaagtc
     2641 tctcgacaca gcgttccaac gtctttcgcg tgcagtcgga accagcggtt tcgtaataaa
     2701 cggttgttcg ttctttcatc atagatgtct ccctcctcaa gtcccctatt atatactacc
     2761 gagggttcaa acggtacacg aagtgtgagt attatatcga gggggaaaaa gaggaaagaa
     2821 gaagagggaa aaaacgggat cacgtacgaa tataatcgca gggtaaccaa cgggctgact
     2881 tcaatgagtc tttggggtta tcgcggggta accaacggga ccccgcgata cgccctttgt
     2941 ccttacacaa aagcgtatcg cgccgtcgcg aaaagaagag gcgcttacgc cgcgatggct
     3001 gtgcagatta tcgcggggta accggcggga tgacattgga agctcacgaa gtctcaaaat
     3061 ctgctttctt gtgacccgcc gtggaaaggc tgaacttccg ggagcgccct gggaatgccg
     3121 aacctccggg cgagccttgg gaacgccgaa ctttagttcg gtatgtttta cggttttaat
     3181 ttcaaaaaac atattgcggc tctagaaaaa acaacggact tgctatataa aagcgctgcg
     3241 ttcgtaaaaa agcaaaaaca tgccaacttt aaagttggcg ttcccaggcg tgcccagacg
     3301 aactcccctc agtgctctct gtgccctccg tgcgaaccct gttttctcgg aacgccggac
     3361 tttagtccgg caggtctttt ctttttctgg gagaaccctg tcccttgcca gttttcctct
     3421 gtgttctccg tgccctctgt gtgagcttct tttcaagaca tgccaactta aaagttggcg
     3481 ttcccaggcg ttcccaagaa tctttcctct gtgttctccg tgctctcagt gcgaaccctg
     3541 ttttctggga acgccggact ttagtccggc aggtcttttc tttttctggg agaaccctgt
     3601 cccttgccag ttttcctctg tgttctccgt gccctctgtg tgagcttctt ttcaagacat
     3661 gccaacttaa aagttggcgt tcccaggcgt tcccaagaat ctttcctctg tgttctccgt
     3721 gctctcagtg cgaaccctct tttttcgcgc cgtcgcgaaa agaagaggcg cttacgccgc
     3781 gatgactaca gagacttaaa accgtgcggt cgattctggt cttgtttctt gagtgcaccc
     3841 catagcttga ccacacccca ttgacattcc catagtcgct tatgatataa tcgggggcga
     3901 ttattttagg ctaacttttg cgcgataatt aatacataat taagatagaa tggctttata
     3961 tcgcaatgta atcataaagg agcggaaaag aatgaaggta aaaagcatcc gtggaaaact
     4021 gttactgtat tttatcccgc tatcggtaat cgtcttagta atcacaggat tggtcgtcgg
//
LOCUS       DAOPXN010000203         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_22461_length_4035_cov_5.592211, whole genome shotgun sequence.
ACCESSION   DAOPXN010000203 DAOPXN010000000
VERSION     DAOPXN010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22461_length_4035_cov_5.592211"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..3156
                     /locus_tag="PKM49_09100"
     CDS             82..3156
                     /locus_tag="PKM49_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY82990.1"
                     /translation="MFTSDSGYLAYMSNRTGEWILEYFNLETRVQYQTKIRGMYPLLS
                     EDDSETVVEEKGHIIVWKTSGAESSTLTKGQTATWITQKTASRFLSAPETTETGKVLA
                     QATIGPQGGSVSAPGAVKLTIPSGVLDRQTPVTIREVASFQPGMRTFDFDFPGAPGVF
                     SDWVTLVYDLPPNTDPERVCAVEEICEGIWCVVPSDYDAKTHTLRTTTAHFSKKGVIS
                     EISASDMRKIFSGAVGALGTAGTIIVITGSTAITLPVLGAVAAGAAIFGAAEVANPLV
                     DKAYELAYGLNRTVVFGDNFNVSWTGDEHSQSYLQTEYLLVSIERASKKMRFWLDDPK
                     ITAQERADAFRALFPFAPVELYQIPKTVISLVAEIQEIKDYYGYNGYACPPSMDIWVH
                     KSDVCGYWDGTKLHVDSEYLQNDDPDTKASRRVVIAHEYWHSVYQHNHYKPNFPWLDE
                     CLATTFESEALSNAELLYSQQDPSALPMSERFYDMYPADKLALTLRTGFVLDGTEGVD
                     ADRAKRGYHLWAWGKFLLHTQGHDTIRELLRNTIDPKLLTAEFSMFCRSLLIKELELE
                     EDGLASIPKTPLLEYITKTGWPFLTVDKLLDTGIISLGNAGAAFTSGVALKPSPLSMT
                     IRNIKGKAPDGEYPLILRRFKPDANEDIIALHPTKKDTESERRSLDDVEVGTGWVVIP
                     PEWTIGLSKKDVEIPVAIVHRAVKQTWAEYFGYGDNPLYCYYLSPPIDLQIVPSGGQF
                     TLRWSEPDFGPGLSAEHCLKGYRLFARKGNQTPVPIGMEIKPKQTSLTLNTASYQNYD
                     KIGLACEDLYAADSQKKGLLSPIAWVDLKTGKGVWILESESYSYQLGRNLFDAETGSG
                     REGAYEDGCFAYRISIGDGQARTSVQITAKDTFCSYWSRKGEISKFLHTWTPLPKQLV
                     PGQKLTIQLGVSDDGCTGKYSPIFNAKTSFRAAGYDVYEVEAGGSFGSGIGTIPWVGR
                     LQKQYEWVVPEPSALEISQPKANPMTISIYFENDAAGAVTGGVFYRYVFHPQ"
     gene            3340..3415
                     /locus_tag="PKM49_09105"
     tRNA            3340..3415
                     /locus_tag="PKM49_09105"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3373..3375,aa:Lys,seq:ttt)
     gene            3425..3513
                     /locus_tag="PKM49_09110"
     tRNA            3425..3513
                     /locus_tag="PKM49_09110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3459..3461,aa:Leu,seq:tag)
     gene            3517..3591
                     /locus_tag="PKM49_09115"
     tRNA            3517..3591
                     /locus_tag="PKM49_09115"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3549..3551,aa:Gly,seq:gcc)
BASE COUNT         1024 a          985 c         1135 g          891 t
ORIGIN      
        1 acttggctta tatgttgaat ccactccccc gtacgattcg acatataagc caagtagccc
       61 gaatccgatg agtactaccc cgtgttcacc tcggattcgg gctacttggc ttatatgtcg
      121 aatcgtacgg gggagtggat tctggagtat ttcaacctag aaacgcgagt ccaataccaa
      181 acgaaaattc gcgggatgta tccgctttta tccgaagacg attctgagac agttgtggaa
      241 gaaaagggcc atatcatcgt ttggaaaacg tcgggggctg agtcttctac tttgacgaag
      301 ggtcagaccg ccacgtggat aacccaaaag acggcgtcaa ggtttttgag cgcgccggaa
      361 actaccgaaa caggaaaggt cttggcgcaa gcgacgatcg gtccgcaagg gggatccgtt
      421 tccgccccgg gggcggtgaa attgaccatt ccttcgggtg tattggaccg tcagaccccc
      481 gttacgatca gagaggtagc ctcttttcaa cctggtatgc gaaccttcga tttcgatttt
      541 cctggcgccc cgggagtgtt ttccgattgg gtgactctcg tgtatgacct cccaccaaac
      601 acggaccccg aacgagtatg cgctgtggaa gaaatatgcg agggcatatg gtgtgtggtt
      661 ccatcggatt acgatgccaa gacacacact cttcgaacga cgaccgcgca tttttcgaaa
      721 aaaggcgtaa tctcggaaat ttctgcttcg gatatgcgga agatattcag tggagcggtc
      781 ggcgccttgg ggactgccgg cacgatcatc gtgataactg gttcgacagc gatcaccctt
      841 ccggttttag gcgcggtggc cgccggagcc gcaatattcg gagcggcgga ggttgcgaat
      901 cctttagtcg acaaagcgta cgagcttgcc tatgggctga atcgtacggt cgtttttgga
      961 gataacttca atgtatcttg gaccggagat gagcatagcc aaagctatct gcaaacagaa
     1021 tatcttttgg tgagtatcga acgggcgagc aaaaagatgc gcttctggct ggacgacccg
     1081 aaaataaccg ctcaagaacg agcggatgcg tttagagccc tttttccttt tgccccagtc
     1141 gagctgtatc agataccaaa gacggtgatc tccttggtgg cagaaatcca agagataaaa
     1201 gactattacg gatataacgg ttacgcctgc cctccatcga tggatatctg ggtgcataag
     1261 tcggatgttt gcggttactg ggatgggacg aagctccacg tggacagcga atatctacag
     1321 aacgacgacc ccgacacgaa agcgtcacgg cgcgtggtta tcgcccacga atattggcac
     1381 agtgtctatc aacacaacca ctacaaaccg aactttcctt ggcttgacga atgcctggcg
     1441 actacattcg aaagcgaagc gctgagcaac gccgagctac tgtactcaca acaggaccct
     1501 tcggcattgc ccatgagcga acgattttac gatatgtatc cggctgataa attggcgttg
     1561 acgctgagaa cagggttcgt cttggacggc acagaagggg ttgatgcgga tcgggccaaa
     1621 agagggtatc acctttgggc gtgggggaaa ttcttactcc atacgcaagg ccacgatacg
     1681 attcgagagc tgctgcgcaa tacgatcgat ccaaagcttt taaccgccga gttttccatg
     1741 ttttgtcgat cgctcttgat aaaagagcta gagctggaag aagatggcct ggcgagtatt
     1801 ccgaaaacgc ctcttttgga atacatcacg aaaaccggat ggccttttct aacggtggat
     1861 aaactactgg acacggggat cattagcctc ggaaacgctg gcgccgcttt cacctccgga
     1921 gtggcgctca aacccagccc actgtcgatg acgattcgga atatcaaagg caaagcgccc
     1981 gatggggagt acccgttgat ccttcgacga ttcaagccag acgcaaacga ggatatcatc
     2041 gcgctccatc cgacgaagaa ggataccgaa tcggagcggc gttcgctgga cgacgtggag
     2101 gtcggaaccg gttgggttgt cattcctccc gagtggacga tcggtctctc gaaaaaagat
     2161 gtggagatac ccgtggccat cgtgcaccga gcggttaaac agacgtgggc ggagtacttt
     2221 ggctacggag acaatccgct ctattgttac tatctgtctc ctcccataga tttgcagatc
     2281 gttccgtctg gcggacaatt cacgcttcgt tggtcggaac ccgactttgg acccggactc
     2341 tccgcggagc attgtttgaa gggctaccgc ctcttcgcgc gaaaaggaaa tcaaacgccc
     2401 gtgccgatcg gaatggagat aaagccgaaa caaacctctc tgacactcaa cacagcatct
     2461 tatcagaatt acgataaaat cggactggcg tgcgaagacc tctacgcggc ggactctcaa
     2521 aagaagggac tcctgtcacc catcgcgtgg gtcgacttaa aaacggggaa aggcgtctgg
     2581 attctcgagt ctgagtccta cagttatcaa cttgggcgca atttatttga tgccgaaacg
     2641 gggagtggtc gagagggcgc ctatgaagac ggatgtttcg cctatcgaat cagcatagga
     2701 gacgggcaag cgagaacctc cgtccagatc acggcgaaag acaccttttg ctcctattgg
     2761 tcgagaaaag gggaaatctc caagtttctc catacctgga ccccgttgcc gaaacaactc
     2821 gtcccgggac agaaacttac gatacagtta ggagtgtccg acgatgggtg taccggaaaa
     2881 tacagtccca ttttcaatgc gaaaacgagc ttccgagcgg ccgggtacga tgtttatgaa
     2941 gtggaagcgg gaggatcttt tggcagcgga atcgggacta taccttgggt gggcaggttg
     3001 caaaaacaat acgaatgggt ggttccggaa ccgagcgcct tagagatcag ccagccgaag
     3061 gcgaatccga tgacgattag catctacttc gaaaatgatg cggccggcgc agtcaccggc
     3121 ggtgtgttct atcgatacgt ctttcatccg caataggcga ctcttcggaa aggggaagac
     3181 gtggggcgcc gagagggctt gaagggaagc gagttgcggg tgtcatcata cgtcgaattt
     3241 acaaggcttt tacaaagcgg agtgaatgaa cgttggatag ggcttgaaat atcggaagat
     3301 ttatgataaa atcactccgc tttcgacaag acggtggtcg ggctgctagc tcagtcggta
     3361 gagcacatga cttttaatca tggggtcctg ggttcgagtc ccaggcggct caccagttct
     3421 tttagcgaga gtggcggaat tggcagacgc gctggactta gaatccagtg ggaagaaact
     3481 cctttatggg ttcaagtccc atctctcgca ccaagcgcgg aaatagctca gcggtagagc
     3541 atctgcttgc caagcagggg gtcgcgggtt caaatcccgt tttccgctcc aaaggaaggg
     3601 cctaaacggc cctcttttgt ttatggtacg gtttttggag gctgggggcg tttccatgcg
     3661 aaacatcgcg gcgtaagcgc cccttctctt cgcgacggcg cgatacgctt ttgcgcttgt
     3721 cctgaaaaac aggggttcgc acagagggca cagagaacac ggagaaaaga ttgaagaaaa
     3781 ctcttggggg cgacttccag tcggcctgtt tcacaataaa aaaacagggt ttcgcacgga
     3841 gaacacagag ggcactgaga taacatagaa cacatgccgc actaaagtgc ggcgttccca
     3901 gggtcttctc taaggagcaa tcgcggcgta agcgcctttt cttttcgcga cggcgcgata
     3961 cgcatttgca catgtcttga aaaacagggc tcgcacagag ggcacagaga acacagagga
     4021 aaaactggca aaggg
//
LOCUS       DAOPXN010000204         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_22901_length_3978_cov_4.629365, whole genome shotgun sequence.
ACCESSION   DAOPXN010000204 DAOPXN010000000
VERSION     DAOPXN010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22901_length_3978_cov_4.629365"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(184..540)
                     /gene="rplQ"
                     /locus_tag="PKM49_09120"
     CDS             complement(184..540)
                     /gene="rplQ"
                     /locus_tag="PKM49_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNY82991.1"
                     /translation="MRHRNKINALSRASDHRKALIHNLMKDLFEHGSIMTTLPKAKAV
                     RPYVEKAITKGKSGTVAARRHLYTFLQDTKLCNKVVDEISKLYMYRPGGYTRILKLGQ
                     RRGDAAEMAILQLVKE"
     gene            complement(556..1665)
                     /locus_tag="PKM49_09125"
     CDS             complement(556..1665)
                     /locus_tag="PKM49_09125"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNY82992.1"
                     /translation="MLKYVMPQKLVVEEQKEDGDYFYGRFILAPLEKGYATTLGNSLR
                     RVLLSSIMALGITKIKFPDKFHEYDSIEGVAEDILEIILNIKRIQLRKELKMDEEIEL
                     SIKKSGPCIVTGKDVLCPAGVTITNPNLHIATLNENARFEVYMYAQIGKGYVPISEQE
                     KPDDIQMIPIDGVFSPVVRVNFRQENVRVLKKTDYDKLVLEIWTKRNIKPTEALRVAS
                     DIMIEHFKIIGSDLEQVEGIDTALFSAPDTSQIADEVEEEIDEKVESIVEDIEEEENE
                     IEAIVPPPVEKAEPPEEKIVEDVHSLSIEKLQLSVRSNNCLKRDKINTIGDLLARAPS
                     ELLKIRNFGQKSLDEVQEKLKTLFHITYEEIEEKK"
     gene            complement(1739..2371)
                     /gene="rpsD"
                     /locus_tag="PKM49_09130"
     CDS             complement(1739..2371)
                     /gene="rpsD"
                     /locus_tag="PKM49_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNY82993.1"
                     /translation="MARYTGPVCRLCRREGKKLYLKGDRCFSTAKCAFEKRPYIPGQH
                     GKSNPKKLTQYGAQLRAKQSMKRIYGVLESQFANYFEEARRRKGVTGEILVKLVESRL
                     DNVVYRMGFAVSRDQARQLVNHGHFKVNGKKVDIPSFLLRVNDVVELSERSRSIKPIQ
                     NAIEAAKTRGTPGWLDIDFDGFKATLLRLPNLEETQLPLDVQSIVELYSK"
     gene            complement(2452..2844)
                     /gene="rpsK"
                     /locus_tag="PKM49_09135"
     CDS             complement(2452..2844)
                     /gene="rpsK"
                     /locus_tag="PKM49_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNY82994.1"
                     /translation="MAQKKPETKKKKKLAVDKGVVHIQSTYNNTIVTISDTYGNTLAW
                     SSGGTVGYSGSKKSTPYAAQLAADQVGKEVVKYGLKRVDVIVKGPGPGREAVIRTLQA
                     AGLEVGTIKDSTPVPHNGCRPKKRRGLQ"
     gene            complement(2859..3233)
                     /gene="rpsM"
                     /locus_tag="PKM49_09140"
     CDS             complement(2859..3233)
                     /gene="rpsM"
                     /locus_tag="PKM49_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNY82995.1"
                     /translation="MARVLGVELPNQKKLFVALRYLYGIGPTRAEKIIAEVHLDRDKR
                     VKDLTEEEIRKITTHINENYKVEGELRQELQKDIKRLIEIGSYRGFRHRLGLPVRGQR
                     TKTNARTRKGHKPGIGRAKSKA"
     gene            complement(3258..3374)
                     /gene="rpmJ"
                     /locus_tag="PKM49_09145"
     CDS             complement(3258..3374)
                     /gene="rpmJ"
                     /locus_tag="PKM49_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNY82996.1"
                     /translation="MKVRASVKPRCDFCKIIRRKGIVKIICSRNRKHNQKQG"
     gene            complement(3405..3632)
                     /gene="infA"
                     /locus_tag="PKM49_09150"
     CDS             complement(3405..3632)
                     /gene="infA"
                     /locus_tag="PKM49_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNY82997.1"
                     /translation="MAKDKNDVIKAEGKVTEALPNAVFKVILDNNHLVTAHISGKMRK
                     NFIRLVPGDTVVVELSIYDLTKGRIIYRKKA"
     gene            complement(3689..>3978)
                     /locus_tag="PKM49_09155"
     CDS             complement(3689..>3978)
                     /locus_tag="PKM49_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNY82998.1"
                     /translation="VERHGFSVIRDYVGHGIGKNLHEDPQIPNFGEPSNGTILREGMV
                     LAIEPMVSVGDWRTDLLRNGWTAVTMDRSLSAHFEDTVALCSNGVRNLTRL"
BASE COUNT          794 a         1075 c          930 g         1179 t
ORIGIN      
        1 aattcacctg atgcgcgggc agctgcataa atttcgagtt gaaggtggcg ctgaccaggt
       61 tttggttgcc catcaccgtc aacccggccg ctagagcgat gacgacgagc ggaaaaccgt
      121 agcgtttgga ggaaaagaaa aggcggcttt cgccacctat ccgatccgtc aaacccaaag
      181 acctcattcc ttaacgagct gtagaatggc catttcggcc gcgtccccgc gtctttgacc
      241 cagcttcaat atccgtgtgt acccccccgg gcgatacata tacagtttgg aaatctcatc
      301 gacgactttg ttgcagagtt tcgtatcctg gaggaatgtg tacaggtgtc ttctggcggc
      361 caccgtgccg ctttttcctt tcgtgatagc cttttcaacg tagggcctga cggccttcgc
      421 cttcggaagg gtcgtcataa tgctgccgtg ttcgaagagg tctttcatca ggttatggat
      481 aagggctttt cgatgatcgc tggccctact taacgcattt attttatttc tgtgccgcat
      541 aaggttcgct cctttctatt tcttttcttc tatctcttcg taagtgatgt gaaacagggt
      601 tttaagcttt tcttgaacct cgtcgagcga tttttggcca aagttccgga tcttgaggag
      661 ttcggaaggc gctcgcgcca gcagatcccc tatggtattg attttatctc gtttcaggca
      721 attgttcgac cgcacggaga gttggagctt ctcgatagat agggaatgaa cgtcttccac
      781 gatcttttct tcagggggct ccgccttttc cacgggaggg ggaacgatgg cttcgatttc
      841 attttcttct tcttcgatgt cttcgacgat gctttcgacc ttttcatcga tctcctcttc
      901 gacttcatcc gcgatttgag aggtgtccgg cgcgctaaac aacgcggtgt cgataccctc
      961 gacctgctcc agatcggatc ctatgatttt gaaatgctct atcataatat cggaagccac
     1021 acgcagggct tccgtcggct tgatattgcg cttcgtccag atctcgagga ctagcttgtc
     1081 atagtcggtc ttcttcagta cccgcacgtt ctcctgacgg aagttaacgc ggacgacggg
     1141 gctgaagacg ccatcgatgg gaatcatttg gatatcatcc ggtttttctt gttcggagat
     1201 cgggacgtaa cccttgccga tctgcgcgta catatagacc tcaaaacggg cgttttcgtt
     1261 caaggtagcg atatggaggt tagggttcgt aatcgtgacg ccggccggac agaggacgtc
     1321 tttacccgtt acgatacacg gaccgctttt ctttatcgac agctcgatct cttcgtccat
     1381 tttcagctct ttccgcaatt ggattcgttt gatattcaag atgatttcca agatatcttc
     1441 ggcaacgcct tcgatagaat cgtactcgtg gaatttatcc ggaaacttga tcttggtgat
     1501 cccaagcgcc atgatcgagg agagcagaac cctgcgcaag gagtttccca aggtcgtcgc
     1561 atagcctttt tccaagggag cgagaataaa tctgccgtaa aaatagtccc cgtcttcttt
     1621 ctgctcctca accactagct tttgaggcat cacgtacttc aacattctct tttccctcct
     1681 gttaaatgat ggtaagaacg gaaaaagaaa ggacgattct tttccatccc cgccagtttt
     1741 acttcgagta caattccact atggattgta cgtctaacgg taactgggtt tcttcgaggt
     1801 tgggaagacg gagtaaggtt gccttaaacc catcgaagtc aatatcgagc cagccgggtg
     1861 tcccgcgggt tttcgccgct tcgatggcgt tttggatcgg tttgatggat ctgctccttt
     1921 cgctgagctc caccacgtcg ttcacgcgca agagaaagga agggatgtcc actttcttgc
     1981 cgttaacctt gaaatgcccg tggttcacca actgtcgagc ttggtctcta gatacggcga
     2041 aacccatacg atagacgacg ttgtcaagcc tggattccac aagtttgacc aagatctccc
     2101 cagtgacacc tttcctccta cgcgcttctt caaagtaatt ggcaaattga ctttcgagaa
     2161 cgccgtagat tctcttcatc gattgtttcg cacgtaattg cgcgccgtat tgagtcaatt
     2221 tcttcgggtt gctttttccg tgttgtccgg ggatgtaggg ccttttttca aaggcgcact
     2281 tggctgtact aaaacagcgg tcccctttca ggtatagttt tttgccttct cttctgcaca
     2341 atctgcagac agggccagta tagcgtgcca ttcagctttc ctcctttaag tcgatagcga
     2401 ctgcgccttt catcgggttc ctatggaaaa tggggcgggg cgtgcccgtc cttattgtaa
     2461 gcctcttctt tttttcggac ggcaaccgtt gtggggaacg ggggtcgaat cctttatcgt
     2521 cccgacttcc aatcccgcgg cttgcaaggt tcgaatgacc gcctctcgtc cggggccagg
     2581 gcccttcacg atgacatcca cacgcttcag cccatacttc accacttctt taccgacttg
     2641 gtcggctgcc agttgggcag cgtagggcgt gctctttttc gagccggagt aacccaccgt
     2701 tcccccgctg gaccaggcca aggtgtttcc gtaggtgtcc gaaatagtga cgatcgtatt
     2761 gttataggtc gactggatat gaacaacccc tttatcgacc gctagctttt tcttcttctt
     2821 cgtttcaggt tttttttgcg ccatcgccgg acctcctctt aagccttgct ttttgcgcga
     2881 ccgattccgg gcttgtgccc tttcctcgtt ctggcattgg ttttcgttcg ttgaccgcgt
     2941 acgggtagtc ccaaacgatg tctgaacccc cgatagctgc ctatctcaat caaccgtttg
     3001 atatcttttt gcaactcttg tcggagttcc ccttccactt tgtagttctc gttgatatgt
     3061 gtggtaatct ttcggatttc ctcttcggtc aggtccttaa cccgcttgtc tcggtccaaa
     3121 tgcacttccg cgatgatctt ttccgcccgc gtcggaccga ttccgtacag gtaacgcaag
     3181 gccacaaata gcttcttttg gttgggtagt tctaccccta acacacgcgc catctacaga
     3241 taacctccct ttttccgtta gccttgtttc tgattgtgct ttctgttccg cgaacagata
     3301 attttgacga tgccttttct tctgatgatt ttacagaaat cgcatcttgg cttaacagat
     3361 gctctcactt tcatggtaaa cactcctttc gattttccac tatctcaggc tttttttcga
     3421 tagataattc ttccttttgt gaggtcgtag atcgacaact ccacgaccac cgtatcgccc
     3481 ggcacgagcc ggataaagtt ctttctcatc ttcccggaga tgtgcgccgt caccagatgg
     3541 ttgttgtcca gaatcacttt gaacacggcg ttcggtaggg cttcggtgac cttcccttcc
     3601 gctttgatca catcgttttt atcttttgcc attctttcct ccgacggctt ggttacgcat
     3661 actgaccccc ctagacacgt tttccgcctc aaaggcgggt tagattccgg acaccattgc
     3721 tgcataaggc cacagtatct tcaaagtgag cggacaacga gcggtccatc gtcaccgctg
     3781 tccacccgtt tctgagcaag tccgttcgcc agtctcccac agacaccata ggctcgatcg
     3841 ccaagaccat accttctcgc aagatcgtac cgttggaggg ttctccgaag ttcggtatct
     3901 gagggtcctc gtgaaggttt ttaccgatcc cgtgtcccac ataatccctg atcacggaaa
     3961 atccgtgtct ttcgaccc
//
LOCUS       DAOPXN010000205         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_23248_length_3938_cov_5.423899, whole genome shotgun sequence.
ACCESSION   DAOPXN010000205 DAOPXN010000000
VERSION     DAOPXN010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23248_length_3938_cov_5.423899"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            495..1286
                     /locus_tag="PKM49_09160"
     CDS             495..1286
                     /locus_tag="PKM49_09160"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNY82999.1"
                     /translation="MRRTPFFLCLIGLLLTACMVAKSPEPSTEEPIFIEDYTEEIDFV
                     DQEGALSAQWEDAVDIPTMMEGEISARFPQYNPIGASDFVLRLNTIFYEAAYALLQPA
                     SSEPTVYICDYRTTYQSPRLTSFRYEYYTYQYPAAHGLSACSGFTIDWEREEILPLHA
                     FFGAHPMWKEVINDCMRAYLHEEEITLFDAAAFEGVTEDQTFYLTDTAFVLVYPPYLY
                     TPGALGILEIPVPFVAVASQLREEYRYTLLPELDLADTEKAEQEG"
     gene            1376..2788
                     /gene="atpD"
                     /locus_tag="PKM49_09165"
     CDS             1376..2788
                     /gene="atpD"
                     /locus_tag="PKM49_09165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNY83000.1"
                     /translation="MDTTKKGRVVSIIGPVVDVRFEHGSLPEIFNALEIFNPESGATL
                     VLEVEQLIGDNTVRTVALDSTDGLVRDAEVIDTGAPIRVPVGSKAMGRMFNLLGNPID
                     ERGPVDIQERWPIHRPAPSIKEQSTEIEILETGIKVIDLVAPFPKGGKIGFFGGAGVG
                     KTVLVMELIRNIAIEHHGFSMFAGVGERTREGNDLYLEMEESGVLQNTVLVFGQMNEP
                     PGARFRVALSALTMAEYFRDVEGKDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQP
                     TLSTDMGELQERITSTKKGSITSVQAIYVPADDITDPAPATTFTHLDASVVLSRQISE
                     LGIYPAVDPLDSNSKILDPNIVGEAHYQVARQVQEVLQRYKELQDIIAILGMEELSEE
                     DKKTVQRARKIQRFLSQPFFVAEKFSGIQGQYVPIGETIRSFRDILDGKYDHLPEQAF
                     YMVGNIDQAVERAKTLKGGE"
     gene            2790..3191
                     /gene="atpC"
                     /locus_tag="PKM49_09170"
     CDS             2790..3191
                     /gene="atpC"
                     /locus_tag="PKM49_09170"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNY83001.1"
                     /translation="MSLFQVRIITPERVVWEGKAQVVTYRTLEGSMGVLSKRAPIMAA
                     LDIAPLKITTENGVEEFAVHGGVLKMDGDLYLIVTDAAENVKEIDIYRAEMAKQRAMD
                     RLEKAKSDIERVRAKASLQKNLLRESLGQKK"
     gene            3201..>3938
                     /locus_tag="PKM49_09175"
     CDS             3201..>3938
                     /locus_tag="PKM49_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HNY83002.1"
                     /translation="MIYRAEPIFVERPWGGPFFRQLFPNCPEGPIGEVWLLSGLEGKS
                     TPLVSFDSNEPEERALSDRTREMIGSELPRFPVLVKLLKAEKWLSVQVHPNDRFARLE
                     EGEPWGKSELWAIVGTDEEALLIDGFKEGLSLESLRYPKNHETFARKIETAIHFVKPE
                     IGEIYEIPSGTVHALGPGVMVIEIQQASELTYRLLDWGRQRPLQVEKAIAATLSEGLR
                     AKRHLWIQGVSNDYFDCTFRSDGTAEGF"
BASE COUNT          970 a          986 c         1082 g          900 t
ORIGIN      
        1 tgtgacttac tgattttctc cacgggtatc cctccagaat aaagtctcat cggtattata
       61 cctcaaaggc gggggtttcc aaaaagcccg cgtgggtcaa gttatggggt gacataacta
      121 aaaggctcat cgcggggtaa ccagaaagct gacttcgtaa gcccacgaag tctcgtgata
      181 cgctctttct ccttctcaaa tgcgtatcgc gccgtcgcag aaagaagaag cacttacgac
      241 gcgattacta gatggacaaa aaccgtgcga tcgtttctgg ccttgtttct tgagtacacc
      301 acataacttg acccacaccg tgccggagcg tgggtatcat atcggggcga aggagcgcca
      361 cgtttcggat ccgctggcct ttcattctca aacaacgaaa tcttgcagag cgtaaacgag
      421 gtgagcggat cgtacacgga tcgtcgcccc tttccgtcta taaagataac gctttttaaa
      481 atggaggagg aacgatgaga cgcacccctt tttttctctg tctgattggg ttgttgttga
      541 ccgcgtgtat ggtggcgaaa agtccggaac cttctaccga ggagcccatc tttatagagg
      601 attatacgga agagatagac ttcgtagacc aggaaggagc cctatcggcc cagtgggaag
      661 atgccgtcga tattcccaca atgatggagg gagaaatatc tgcgcgattt ccccaataca
      721 acccaatcgg ggcaagcgat ttcgtattga ggttgaacac gattttttac gaggcggctt
      781 acgcccttct tcaacccgcc agttccgagc caacggtcta tatatgcgat taccgaacga
      841 cctatcagtc accgcggctg acgagttttc gttacgaata ctatacctat cagtaccccg
      901 cagcgcacgg gttgagcgct tgttcgggtt tcaccatcga ttgggagcga gaggagatct
      961 tacctctaca cgcgtttttc ggcgcgcatc cgatgtggaa agaggtgatc aacgactgta
     1021 tgcgcgccta cctgcacgaa gaagagatca ccctgttcga tgccgctgcc ttcgaagggg
     1081 tcacggaaga ccaaactttc tacttgacag acacggcctt cgtgttggtc tatccacctt
     1141 atctatacac tccgggagcc ctaggtattt tggagatacc ggtccctttc gtcgccgtcg
     1201 cgtctcaatt acgggaggaa tatcgatata ccctgcttcc cgagctggat ttggcggaca
     1261 cggagaaggc tgagcaggaa ggataattcc cgccttttcg caaaccgaat acccggcttt
     1321 atggtatact aatatcggaa agaacaagca attacctact cctgggggtg ttcgtatgga
     1381 tacaacaaag aaaggccggg tcgtgagcat tataggaccg gtggtcgatg ttcgcttcga
     1441 gcacggttcg cttcctgaaa tattcaatgc tttggaaatc ttcaaccccg agtcgggcgc
     1501 cactctggta ctcgaagtcg agcagctgat cggcgataat accgttcgaa cggtggcctt
     1561 ggattcgacc gatggattgg tcagagatgc agaggtgatc gacacgggcg cgccgattcg
     1621 tgttccggtt gggtcaaaag cgatgggaag gatgttcaat ctgctaggga acccaataga
     1681 cgaaagagga cccgtagata ttcaagaacg ctggcctatc catcgtcccg ctccttccat
     1741 caaagaacag tctaccgaga tagagatatt ggagaccggt atcaaggtca tcgatctggt
     1801 cgcccctttc ccgaaaggcg gcaagatcgg cttctttggc ggggcagggg ttggaaaaac
     1861 cgtcctcgtg atggagctga tccgaaatat cgcgatcgag caccatgggt tttcgatgtt
     1921 cgcgggcgtg ggcgaacgaa cgagagaagg gaatgacctc tatctagaaa tggaagagtc
     1981 cggcgtcctt caaaacaccg tcttggtgtt cggtcagatg aacgaacccc ccggcgcccg
     2041 attccgtgtc gccctctccg ctttgacgat ggccgagtat tttcgggatg tcgaaggcaa
     2101 agacgttctg ctctttatcg acaacatttt ccgtttcgtc caagccggct ccgaagtgtc
     2161 ggccttgttg ggaaggatgc ccagtgccgt cggataccag cctacgcttt caaccgatat
     2221 gggagaatta caggagcgta tcacctccac gaaaaaagga tcgatcacct ccgtgcaagc
     2281 tatctatgtt ccggcggatg atataacaga cccggctccg gccacgactt tcacccattt
     2341 ggacgcttct gtcgttctgt cgcggcagat ttcggagtta gggatttatc cggcggtcga
     2401 tcccttggac tccaactcga aaatattgga tcccaacatc gtcggagaag cccactatca
     2461 ggtggcgcgc caagttcaag aggtgttgca acgctacaaa gaacttcagg atattattgc
     2521 catcttgggg atggaagagc tttcggaaga ggacaagaag acggtgcaac gggcgcggaa
     2581 gattcaaagg ttcttaagcc aacccttctt cgtcgcagaa aagttttcgg gtatacaagg
     2641 tcagtacgtt ccaataggtg aaaccatccg aagtttccgg gatatcctgg acggcaagta
     2701 tgaccatcta cccgaacagg ccttttatat ggtcggaaat atcgatcagg cggtagaacg
     2761 cgcgaagacc ctcaaagggg gtgaatgatt tgtctctttt ccaagtccgg attatcactc
     2821 cggagagggt cgtttgggag ggaaaggctc aagtcgttac ctatcgaacc ctggaaggat
     2881 ctatgggcgt actttcgaag cgggcgccga tcatggctgc tttggatatt gccccgctga
     2941 agattaccac cgaaaacggc gtggaggagt tcgccgtaca cggtggcgtc ttgaagatgg
     3001 acggagacct gtatctgatc gtgaccgacg cggccgaaaa cgtgaaggag atcgacatat
     3061 accgcgccga aatggcgaaa caacgcgcga tggatcgcct ggaaaaggca aagagcgata
     3121 tcgagcgcgt tagagccaaa gcttctttac aaaaaaacct gctacgcgaa tccttgggac
     3181 agaaaaaata ggatttgtcg atgatctatc gcgcagaacc tattttcgta gaaagacctt
     3241 ggggaggccc tttctttcgg caactctttc cgaactgccc ggagggtccg ataggggaag
     3301 tgtggctgct atcgggctta gaaggaaaat ccaccccgtt ggtatcgttc gactcgaacg
     3361 aaccggagga gcgcgccctc tcagatcgaa cccgagagat gataggctcc gaactcccga
     3421 ggttccctgt gttggtgaag ttgcttaagg cggaaaaatg gctttcggta caggtccacc
     3481 cgaacgatcg cttcgcgcgc ctggaagaag gagagccttg gggcaagtcc gaactgtggg
     3541 cgatcgttgg aacggacgag gaggctctgc tgatcgacgg gttcaaagaa ggcctttctt
     3601 tggaatcctt acgttaccct aagaatcacg agaccttcgc tcgaaagatc gaaacggcga
     3661 tccatttcgt taaacctgag attggagaga tctatgagat cccctccggg actgttcacg
     3721 cgttaggccc cggagtaatg gtcatcgaaa tccagcaagc ctcggaattg acttaccggc
     3781 tgctcgattg ggggcgccaa cgccctcttc aggtcgaaaa ggcgatcgcg gcaacgcttt
     3841 cggagggctt acgcgcgaaa aggcacctat ggattcaagg cgtgagcaac gactacttcg
     3901 actgcacttt tcgatcggac ggaaccgcag aaggcttt
//
LOCUS       DAOPXN010000206         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_23859_length_3861_cov_5.264057, whole genome shotgun sequence.
ACCESSION   DAOPXN010000206 DAOPXN010000000
VERSION     DAOPXN010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23859_length_3861_cov_5.264057"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(407..>3861)
                     /locus_tag="PKM49_09180"
     CDS             complement(407..>3861)
                     /locus_tag="PKM49_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNY83003.1"
                     /translation="LRKWLLSPFVALLVESWANINRIIGSIACQLAFCLEDYGQILKD
                     KVFKKEKRYSNPMIEPRKLFEELIIDPFTHLKVQRKDLFTQPVTLVIDGPDDDEGLEI
                     KHEMAQLIRDRELQNKTPAWIRWIITSRCELLILLLYKGTPIQVDLYIEENQRADFRA
                     YYEKRLSGLLPEQKRENILETLVNRAGGSFLYCELMCDDIERDPRMLRSPKKFPEGMN
                     GYLNRFFSVRFPQGLDDRTRDVLSLMCAAVEPLEADLIRKITGMKKTEFEKCLRAVDP
                     LVLYEEKTETVCFYHTIVRTWLTWEQGGLQPDIYEIEPEKGHRMLADYGWDYLQKKHD
                     WKEPLDYVIENLGIHLYASSKKPLKYKTIRDRLLELFQKSFSGEEKARLPETVIADLL
                     SYVIAHWSEDYETGFKGIVDRFLKSETQPEAKHALADFLHNRCNYDSKRRDTGWARWM
                     LQRQRSVIEELVEAHPENTEWLWDLGESWSKIGDIEKKWENLDQARDAYEKALAIMLE
                     LTAKDPANTDWLHDLGLRWCIVGDIEKGRGNLNLARDACEKFLAIMLELTAKDPDDAV
                     LLENLSVSWNDFGDIEAELLNLDQALKAYEKCLEILLDLTEKDPANTLRQLVLGVVFG
                     KAAGIEAKLGNLTRARENYEKALATTLELIAKDPANTRWQSNLCLDWERMGDLEEQLG
                     NLDRARDAYAKALEIRLDLTAIDPANTDWLQGLYQNWDKIGGIEEARGNLDKALEAFE
                     KSLGITLDLTEKYPTRDVWQLALGASLHRIGGIEEARGNLDQALEAFEKDLAHKLELA
                     KKDPANTDWLRGIDVSWNKIGDIEEKRGNLVQALEAYTKSMETNLVLTKKDPTNTLWL
                     HDLSVSWNKIGDIEEKRGSLPQARDAYKKAQTILRKLTKKDSTNTDWLRDLGVTWSKI
                     GDIDAAQGNRAQARNAYGKDLASLFELTEKDPANTDWLLDLSVSWSKIGDIEEARGKP
                     YQALEAYEKALSILLELTEKDPANTDWLLELSASWVKIGDIEKAQGNLPEAQDAYEKD
                     LAIKLELTEKDPENTDWQRSYGISLSRLGIFHQQQGDTIRAKEYLLRVLELRRSLVDR
                     APQEIDYNIGLVYALLGLYSVVENEEEKREYWGEASGITKYLAGKGVEHGQLDALKKI
                     FGIE"
BASE COUNT          741 a         1107 c          874 g         1139 t
ORIGIN      
        1 aaacttcgta accgagatgt tccaggtctt ctttcaatcg ctgcgccact tccggtagct
       61 cgtgcgggct gtagctgacg tagacgtatt tgttctcctc attgctttga tttggcataa
      121 atatcactcc cgttttgttt ttgaagcaaa accgctgaag gtcttcgagc gcctaccttg
      181 cccgtttaaa ccccgaaaat ctaacggttt ggttcgaact tctctcaaca gcgttttcca
      241 cgttctttcc ttatcggcgc ctccaatatc attggggttc cggtctccga gttttttgta
      301 ctccaaagcg cgtctgcttc cggaagaaga gccctaattt acttattgct cgttcttccg
      361 ttcggtttcg agtgttttta gctactcagc gcggctcgtg ttcgcttcac tcaatcccga
      421 agattttttt cagcgcgtcc agttggccgt gctcaacccc tttgcccgcg aggtatttcg
      481 tgataccgga ggcttctccc caatattccc gcttttcttc ctcattctca actacggaat
      541 aaaggcccag gagcgcataa actaagccta tattataatc tatctcctgt ggcgcccggt
      601 cgactaagga tcgacggagc tccaacactc taagtaggta ttctttcgcc cgtatcgtat
      661 ccccttgttg ttggtgaaat attccaagtc ggcttaacga aatcccataa ctgcgctgcc
      721 agtctgtgtt ctcggggtct ttttccgtca gttcgagctt aatcgccagg tctttctcat
      781 aggcgtcctg cgcctctggc aggttcccct gcgctttttc aatatccccg atcttgaccc
      841 agctcgcgct gagttcgagt aaccagtcgg tgttcgccgg gtctttctcc gtcagttcga
      901 gcaagatcga tagggctttc tcgtaggcct ccaacgcctg gtagggtttc ccccgcgctt
      961 cttcgatatc tccgattttg ctccaactca cgctgagatc gagtaaccag tcggtgttcg
     1021 ccgggtcttt ctccgtcaat tcgaacaagc tcgccaagtc cttcccgtag gcgttccgcg
     1081 cctgggcgcg gttcccctgc gccgcgtcga tatccccgat tttgctccag gtcacgccta
     1141 agtcccgcag ccagtcggtg ttcgtggagt ctttcttcgt cagtttgcgc aagatcgtct
     1201 gcgctttctt gtaggcgtcc cgcgcctgcg gcaggctccc cctcttctct tctatatccc
     1261 cgatcttgtt ccagctcacg ctcaggtcgt gtagccagag ggtgttcgtg gggtcttttt
     1321 tggtcaatac caagttggtt tccatagatt tggtatatgc ttccagcgct tggaccaggt
     1381 tccctctctt ctcttctata tccccgatct tgttccagct cacgtcaatg ccccgcagcc
     1441 agtcggtgtt cgcagggtct ttcttagcca gttcgagctt atgcgccagg tctttttcga
     1501 aggcttctag cgcctgatcc aggttccccc gcgcttcttc gatacccccg atcctatgca
     1561 agctcgcgcc gagggccagc tgccagacat cgcgcgtcgg gtatttttcc gttaaatcga
     1621 gtgtaatccc taggcttttc tcgaaggctt ccagggcttt gtcgaggttt ccccgcgctt
     1681 cttcgatacc cccgattttg tcccagttct ggtacagacc ctgtaaccag tcggtgttcg
     1741 cggggtctat cgctgtcaga tcaagcctga tctccagagc tttcgcatag gcatcccttg
     1801 ctctgtccaa gtttcccaat tgctcttcca aatcccccat tctctcccaa tccaaacaca
     1861 aattcgactg ccaacgggta tttgccggat ctttggcaat caattcgagt gtggtagcca
     1921 gggctttctc gtaattctcc cgcgcccgcg tcaggtttcc tagcttcgct tcgattcccg
     1981 ccgctttgcc aaaaaccacg ccgaggacca gctgcctgag agtgttcgcg gggtctttct
     2041 ccgttaaatc aagcaagatc tccaagcatt tctcgtaggc tttcagggct tgatccaagt
     2101 tcaacagctc cgcttcaata tccccgaaat cgttccagct cacggacagg ttctctagca
     2161 agacggcgtc atcggggtct ttcgcagtca attcgagcat aatcgccaag aatttttcgc
     2221 aagcgtctcg cgccaagttc aggttccccc gtcctttttc gatatctcca actatgcacc
     2281 agcgcaggcc gagatcatgc agccagtcgg tgttcgcggg gtcttttgct gtcaattcga
     2341 gcataatcgc cagcgctttc tcgtaggcgt cccgcgcctg atccaggttc tcccactttt
     2401 tttcgatatc cccgatcttg ctccagcttt cgccgagatc ccacaaccat tcggtgttct
     2461 ccggatgcgc ttccacaagc tcttcgataa cggaacgctg cctttgaagc atccatcgcg
     2521 cccatcccgt atccctgcgt ttgctgtcgt aattacaacg attatgaagg aagtcggcta
     2581 aggcgtgttt tgcctccggc tgcgtttctg acttcagaaa acgatccacg atacctttga
     2641 atcctgtttc gtaatcttct gaccaatgtg cgatcacata gctcagcaga tcggcaatga
     2701 ctgtttccgg aagccttgcc ttctcctctc ccgaaaagct tttctggaac aattcaagca
     2761 accggtcgcg gatcgtcttg tacttcaacg gtttcttact agaagcgtac agatgaatcc
     2821 ccaggttttc tatcacgtaa tctaggggct ctttccaatc gtgctttttt tgaaggtaat
     2881 cccagccata gtcggcaagc atccggtggc ctttttccgg ttcgatttcg tagatgtcgg
     2941 gctgtaaccc tccttgttcc caagttagcc atgtcctcac gatggtgtgg tagaagcaca
     3001 ccgtctctgt cttttcttcg tatagaacca gagggtctac cgcgcgcaag catttttcga
     3061 attcggtttt cttcatcccc gtgattttcc gaatcagatc ggcttccaat ggctcgaccg
     3121 ctgcgcacat cagcgataaa acatctctgg tacggtcatc gagtccctga ggaaaacgca
     3181 cgctgaagaa tcggttgagg tatccgttca tcccctctgg gaacttcttg ggtgaacgaa
     3241 gcattcgggg gtctctctcg atgtcatcgc acataagctc gcaatacaaa aagctacccc
     3301 ctgccctgtt taccagtgtc tccaaaatgt tttcccgctt ttgttctggg agtaagccgg
     3361 aaagtctttt ctcgtagtaa gctctgaaat ctgccctttg gttttcttca atatacagat
     3421 cgacctggat cggggtgcct ttatatagta aaaggattag gagttcacat cgagaggtga
     3481 tgatccaccg tatccaggcg ggtgtcttat tttgtagttc acggtctcgg atcagctgag
     3541 ccatttcgtg ttttatctcc agaccctcat catcgtcggg cccgtcgatc accaaggtca
     3601 caggttgggt gaacaggtcc tttcgttgaa ctttcaggtg cgtgaaaggg tcgataatca
     3661 gttcttcgaa aagttttcga ggttcgatca ttggattcga gtaccgtttt tcttttttga
     3721 agaccttgtc tttgagtatt tgtccatagt cttccagaca aaaagcgagt tgacacgcga
     3781 ttgaaccgat gatccgattg atgttggccc aactttcgac taaaagtgct acaaacggcg
     3841 ataaaagcca ctttctcaaa a
//
LOCUS       DAOPXN010000207         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_24264_length_3814_cov_5.841979, whole genome shotgun sequence.
ACCESSION   DAOPXN010000207 DAOPXN010000000
VERSION     DAOPXN010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24264_length_3814_cov_5.841979"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(399..809)
                     /locus_tag="PKM49_09185"
     CDS             complement(399..809)
                     /locus_tag="PKM49_09185"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY83004.1"
                     /translation="MPQSLKVPATMAHFEESVGFIETHLRGAGVDEKTLTKILTASEE
                     IIVNIISYAYQDGEGDFEIEIEDHPDRTTLSFTDEGQPFNPLQNPDVDTTLAANEREI
                     GGLGILMVKKLMDDVRYRFLDGKNTLTITMVKGA"
     gene            complement(830..1132)
                     /locus_tag="PKM49_09190"
     CDS             complement(830..1132)
                     /locus_tag="PKM49_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012820215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNY83005.1"
                     /translation="MEIKRSVNGNTVTFALSGRLDTITSPQLSKEIEAAFEKEPVNLV
                     FDFSALDYISSSGLRVLLSAQKKVNALGTKMTVKGVNDTIKEIFEVTGFTGILTIA"
     gene            complement(1150..1743)
                     /locus_tag="PKM49_09195"
     CDS             complement(1150..1743)
                     /locus_tag="PKM49_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83006.1"
                     /translation="MKSDIFQLDGSALLTEQALDKVEKLAIYFGFDDKDVAFSRLLAE
                     EGINALESLLGINKGSLWVETTDKDFEVHLKANAQLSTDQREELIDLSKAKRNTPKKG
                     ILGRISRLVDFIATDMVVGENPFIFSNMADEMLSYSAIMASPDMIYWSLQKTKRIEEE
                     KGEGADIERSIIEKFADDIVVSIALKSIEITIKKSKK"
     gene            complement(1740..3656)
                     /locus_tag="PKM49_09200"
     CDS             complement(1740..3656)
                     /locus_tag="PKM49_09200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY83007.1"
                     /translation="MRKTFLVWLFTFLIIAFICALVVSFWALTAQASQNARTLIRLKI
                     ADVEKQLRKNQENLYEIRKEMDESGIAKAKAVAKMIQLNPALIGNLEEIQKIRDLLLV
                     DEIHIVDRRGILIFGSVPEYFNYDMASDPQSAAFLAAIRDPDFALAQDPLPKGINKEI
                     FQYVGVARLDEPGIVQIGYRPEKLARALEIADIRNLAPGFRIGSSGNILILDQNVVIV
                     SSADDRLLGLPSTTLLDEATAQAVKEQMEKEPEGYFTAAANGEKRLFGYKKSGAYYIV
                     GQLPTQEMYLSRDSGTALLVIFNILLFGVIFFVIARLVQSIVINGIYRVNNSLEKITR
                     GNLNEVVSVRTNKEFASLSNGINTTVNALKDAINEASSRIDTELAFAKAIQHSALPST
                     FPAFPDRKDFDIYAQMYTAKEVGGDFYDFFLVDSDHLAVVIADVSGKGIPASLFMMIS
                     KSIIKTYALSGLSVSDILYKSNIALCENNQEDMFVTAFMGILNTRTGVMRYVNAGHNP
                     PLVRKDNGLYEWLPVKQEFVLAGLPNVRYTEQEITFGPRDTLFLYTDGVTEAMNRVEQ
                     LYSPKRLIRVLNQFPVSLGLERTIVGIKEDVDHFAEGTEQADDITMLMLRYDQNEEIS
                     HKENPGNPAKEDFI"
BASE COUNT          869 a         1022 c          935 g          988 t
ORIGIN      
        1 tacgccgcga tgccgcgctt gggaacgcct gggaacgcca acttttaagt tggcaggtct
       61 ttgaggctct tcgcgcttaa gagcgcacaa agttacaaaa aaaagagcca cacaaaaacg
      121 gatcgcgccg tcgctagaag aaaaagcgct tacgccgcga tgccacgctt gggaacgcct
      181 gggaacgcta acttttaagt tggcatgtct ttgagctttc atgtttaaga gcgcataaag
      241 ttacaaaaaa agagccacac aaaagcgtat cgcgccgtcg caagaaaaag aagcgcttac
      301 gccgcgatga ctacgaggtt cttcgacccc ctgaaaagcc gacgttctta gtagcgctct
      361 caggtgttcg gcgctcccag gcagggtgaa atctacgttc aggcgccttt caccatcgta
      421 atcgtcagcg tgttttttcc atccaaaaag cggtagcgga catcgtccat caatttcttc
      481 accatcagga tgcccaggcc tccgatttcc cgctcgttcg cggccaaggt cgtgtcgaca
      541 tcgggatttt gtagggggtt gaacggttgc ccttcgtccg taaaactcag cgtcgttcgg
      601 tccgggtggt cttcgatttc gatctcaaaa tccccttctc catcctgata ggcgtaagag
      661 atgatgttca cgatgatctc ttccgaagcg gtgagtattt tcgtgagcgt cttttcgtcg
      721 acaccggccc ctcgaaggtg cgtttcgata aatccgacgc tttcttcgaa gtgggccatc
      781 gtagccggaa ccttcaggct ctgtggcata aggcaggcat ccgaactgtt tacgcgatgg
      841 tcaagatacc ggtgaatccg gtgacttcaa agatctcttt gattgtgtca ttcacgcctt
      901 tgacggtcat cttcgttcct agcgcgttga cctttttttg agcggagagc aataccctca
      961 accctgagct gctgatgtaa tccagcgcgg agaagtcgaa gaccaaattg acgggctcct
     1021 tttcgaatgc tgcctctatt tctttgctca attgcgggga agtgatggtg tccaatcgcc
     1081 cggagagggc aaaggtcacc gtgttcccgt tgacactcct ttttatttcc atcgcactcg
     1141 cctcctattt tacttctttg atttttttat ggtgatctct atgcttttca acgcgatcga
     1201 taccacgatg tcgtcggcga atttttctat gatagaccgc tcgatatccg cgccttctcc
     1261 tttctcttcc tcaatccgct tggtcttctg cagagaccag tagatcatat ccggagaggc
     1321 cattatcgcg gagtatgaga gcatttcatc cgccatattg ctgaaaatga acgggttctc
     1381 tccgacaacc atgtctgttg cgatgaagtc gactaagcgt gatattctac cgagaattcc
     1441 tttttttgga gtattccttt tcgctttaga tagatcgatc agctcttctc gttgatcggt
     1501 ggacaactgc gcgttcgctt tgagatgcac ttcaaaatcc ttgtccgtcg tttccaccca
     1561 caacgatccc ttattgatcc ccagtaacga ttccaacgcg ttgatcccct cttcggccaa
     1621 cagtctggaa aaagcgacgt ccttgtcatc gaagccgaaa tagatcgcca acttttccac
     1681 tttatccaac gcctgttcag taagaagagc ggaaccgtcc aattggaaaa tatccgattt
     1741 catatgaaat cctcctttgc ggggttaccc ggattttctt tatgagaaat ctcttcgttt
     1801 tgatcgtaac gcagcatcaa catcgtgatg tcgtccgcct gctccgtccc ttcggcaaag
     1861 tggtccacat cttctttgat gccgacgatc gtccgctcca atcccagaga tacggggaat
     1921 tggttgagaa cgcgtatcag gcgtttcgga ctgtataatt gctcgactcg gttcatcgcc
     1981 tcggttacgc cgtcagtata cagaaagagg gtgtccctag gcccaaaggt aatctcctgc
     2041 tccgtatacc gaacattcgg aagccccgcg agaacgaatt cctgcttcac cggcaaccat
     2101 tcgtagaggc cgttgtcctt ccgaaccagc gggggattgt gtccggcgtt cacataccgc
     2161 atcactcctg tccgcgtgtt caatatcccc ataaaagccg tgacgaacat atcctcctgg
     2221 ttattctcac acaacgcgat attggacttg tacaagatat ccgagaccga cagtccggag
     2281 agcgcatagg tttttatgat cgacttcgag atcatcatga agagcgaagc cggaattcct
     2341 tttccagaga cgtccgctat tacgacggcc aagtggtccg aatccaccaa aaagaagtcg
     2401 tagaagtcac cgcccacttc tttagccgta tacatctgcg catagatatc gaagtctttg
     2461 cgatccggga aagcgggaaa tgtcgaaggg agggcggaat gctggatcgc tttggcgaag
     2521 gcgagctccg tatcgattcg agaagacgcc tcgttgatcg catctttgag cgcattcacc
     2581 gtggtgttaa tcccgtttga caaagaagcg aactccttgt tggtgcgcac ggagacgacc
     2641 tcgttcaggt tcccgcgcgt gatcttctcc agagaattgt tcacacggta aatgccgttg
     2701 atgacgatag actgcaccaa cctcgcgatg acgaagaaga tgactccaaa caaaaggatg
     2761 ttgaagatga ccaaaagggc cgtgccgcta tcgcgcgaca gatacatctc ttgggtcggc
     2821 aactgtccga cgatatagta agccccgctc tttttgtatc cgaacaagcg tttctctccg
     2881 ttggccgccg cggtgaaata gccttctggc tctttttcca tctgctcttt caccgcttgc
     2941 gccgtcgcct cgtcgagcag cgttgtcgaa gggagaccca acaaccggtc atcggccgaa
     3001 ctcacgatga ctacattttg atcgaggatg aggatgttcc cagagctccc aattcgaaac
     3061 cccggagcca aattcctgat gtcggcgatc tcgagggctc gggccagttt ctctggccgg
     3121 tagccgatct gcacgatccc gggctcatcc agcctcgcga caccgacgta ctggaagatc
     3181 tctttgttga tccctttcgg taaggggtct tgggccagag cgaagtcggg gtcccgtatt
     3241 gcagccaaaa aggctgccga ttgcggatcg gaggccatat cgtagttgaa atactccggg
     3301 acgcttccaa agatgaggat gccccgccga tccacgatgt gtatttcgtc aacaagcagc
     3361 agatctctga ttttctgtat ttcctcgaga tttccgatga gggcgggatt cagctggatc
     3421 atcttcgcca cggcctttgc cttcgcgatt ccgctctcat ccatttcttt tcgtatctcg
     3481 tagaggtttt cttggttttt tcgcagctgt ttttcgacat cggcgatctt tagtcgaatc
     3541 agcgttctgg cgttttgaga ggcttgagcc gtcagcgccc aaaaagaaac cactagggcg
     3601 cagatgaagg cgatgatcaa aaacgtgaac agccaaacca aaaaggtctt tcgcatagcg
     3661 aatccctatc ttcctttccc ccggatagga aagagttcct aacatccgca gattatatca
     3721 cagtcttcca gcgtgtgggt caagctatgg ggtgacaaga ctaagaggtt catcgcgggg
     3781 taaccagcgg gctgacttcg gaagcccacg aagt
//
LOCUS       DAOPXN010000208         3766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_24693_length_3766_cov_5.136621, whole genome shotgun sequence.
ACCESSION   DAOPXN010000208 DAOPXN010000000
VERSION     DAOPXN010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24693_length_3766_cov_5.136621"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2633
                     /locus_tag="PKM49_09205"
     CDS             <1..2633
                     /locus_tag="PKM49_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNY83008.1"
                     /translation="TSTEWTFTTLAPVYGPPTIELTGPATGATDEGTKVTLTWEATPG
                     ELKAAGKRDGVTITGYEVYHKKTGDPDPTKPATTADNSYELENLEYGTEYTWKVVALQ
                     SDGQRATSTARTFTTMESLYNPPMLALTSPASRATNQATTVTLTWEATPGVQKIVPAG
                     DRDMTISHYLVYFAAENAEYGEPEATDVKQMQKTGLEYDTTYKWKVKAVQFDGQSKTS
                     DESTFSTLAPVYGPPTIELTSPASGATDRPTTLTLTWVATPGAQTNVGERAVSLTFRV
                     YYQKEGGTKASTETGNPYYNLTDLDPEATYTWQVVAVQSDGQHATSTERTFKTRAGGA
                     TETPIKRYNSSGALQKEYAKLSEAIADAVNGDRIDVDGGKVLDNEDKQVTIDGKQITI
                     RSSPTNNPFTIDMTELNKQGSNRGNNRVLEITGGASVTIRDAIITGGSLEGNDVGGGI
                     CITGGSNVTTFNVTVNDNTAGRGGGVGVYLQGAFNAHDTTITKNTAREKGGGVYVQTG
                     AFNAYGTAITENTAQFFGGGGVFVFDGTFNAYEGTTIRENTAEKNGGGGVYVVTNGTF
                     NATETTIASNTAEKSNGGGVYVFEGIFNAYEGTTITKNEADVGGGVGVYGNNGTFNAF
                     NTTITENIANYYAGGVGVARGIFNDYHSTISSNFAFGAGGVGVAYGTVNSENTIITRN
                     EAFEGGGVGVAAEGVFNARNTRIESNKGYESAGGVGVTLGGVFNAEESVTIKGNRADS
                     GNPEDFPSFGGGVFVAEGGIFYAKNNTTIESNEADYGGGVCVTGPHDGTERGNFYAES
                     VTIKENRAKNVGGGIAWVTGISEIRTRVNTWTPASDQEQEFSVTTTGEIHAKTTGDPV
                     QVFENTSEKGGSQMKVFGNP"
     gene            2673..3143
                     /locus_tag="PKM49_09210"
     CDS             2673..3143
                     /locus_tag="PKM49_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83009.1"
                     /translation="MKRFLKIVILSSITAMVFLLSSVYECHGGDIIGESYGSNTVVDF
                     RNYSEKRIKIMFPKNKHESGEVIIPGCDPNKMGPVYHRMGFQQGYFDPPLRFFIFKEN
                     DEGDWDPLNNLTVGEFKSKGDEAVSEKNLFRISDSGYKKYSRQHGASDWWQEVP"
BASE COUNT         1024 a          857 c         1057 g          828 t
ORIGIN      
        1 cgacgagtac cgagtggacc tttacgacgc tggctccagt gtacgggcca ccgacgatag
       61 agctcacggg gcccgcgaca ggcgcgacag acgaggggac gaaggtaact ttgacgtggg
      121 aggcgacacc tggagagcta aaggctgcgg ggaaacgcga cggagtgacc atcacagggt
      181 atgaggtgta tcataagaaa acgggagacc cggatcctac gaaacctgcc acaactgctg
      241 ataactccta tgaactggaa aatcttgaat acgggacaga atacacgtgg aaagtcgtgg
      301 ctctccaatc ggacgggcaa cgtgcgacaa gtacagcgcg aacgttcacg acaatggagt
      361 ctctgtacaa tccaccgatg ctcgcactca cgagccccgc gagcagggcg acgaatcagg
      421 caacgaccgt gactttaacg tgggaagcga caccgggagt gcaaaagatc gtccccgcgg
      481 gcgaccgcga tatgacgata tcccattatc tcgtctattt cgcggctgaa aatgcagaat
      541 acggcgaacc agaagccacg gatgtgaagc agatgcagaa aacgggcttg gaatatgata
      601 cgacctacaa gtggaaagtg aaagcggttc agtttgacgg gcaatcaaaa acgagcgatg
      661 aaagcacatt ctcgacgctg gctccagtgt acggaccacc gacgatagag ctcacgagcc
      721 ccgcgagcgg cgcgacggat cggccgacga cgttgacgtt aacgtgggtg gcgacaccgg
      781 gcgcacaaac gaacgtggga gagcgagcgg tgagtttaac attccgcgtc tattatcaaa
      841 aagagggcgg taccaaagca tctactgaga cggggaatcc atactataac ttgaccgacc
      901 tcgaccccga agccacatac acgtggcagg ttgtggcggt gcaatcggac gggcaacacg
      961 cgacgagtac tgagcggacg ttcaagacgc gcgcaggagg ggccacagaa acgcctatca
     1021 aacgatataa ctcatctgga gcgctacaaa aagaatatgc caaactcagc gaggcgatcg
     1081 ccgacgcggt aaatggagac cgcatcgacg tggacggcgg aaaggtattg gataacgaag
     1141 ataaacaagt aaccatcgat ggaaagcaga taacgatccg ctcttcgcct accaacaatc
     1201 cgtttactat agatatgact gaactaaaca aacaaggttc caaccgagga aacaaccgag
     1261 ttttagaaat tactggcgga gcgtcggtga ccatccgcga tgcgatcatc accggtggga
     1321 gtttggaagg taacgatgtc ggcggcggga tatgcatcac cggaggtagt aacgtcacga
     1381 ccttcaatgt gacggttaat gataataccg caggccgagg cggtggcgtg ggcgtctact
     1441 tgcaaggcgc ttttaacgct cacgacacga cgatcacaaa gaacactgcg agagagaaag
     1501 gcggcggcgt gtacgtccaa accggcgctt ttaacgctta cggcacggcg atcacggaga
     1561 acaccgcgca attttttggg ggcggcggtg tgttcgtctt tgacggcact ttcaacgctt
     1621 acgagggcac aacgatcagg gagaacaccg cagaaaaaaa tggcggcggc ggtgtgtacg
     1681 ttgtaactaa tgggactttc aacgcgacgg aaacgacgat cgcgtcgaac actgcggaaa
     1741 agtctaacgg cggcggtgtg tacgtctttg agggtatatt caatgcctac gaaggcacga
     1801 cgatcacgaa gaacgaagct gatgtgggcg gcggagtggg tgtctatggg aataatggta
     1861 ccttcaacgc ttttaataca acgatcacgg agaacatcgc gaattattat gccggtggag
     1921 tgggtgtcgc gcgtggcatt ttcaatgatt accattcgac aatctcatcg aacttcgctt
     1981 tcggagctgg cggagtgggt gtcgcttacg gcacagtcaa ttccgagaac acgataatca
     2041 cgcggaacga agctttcgaa ggaggcggag tgggtgtcgc agcagagggc gttttcaacg
     2101 ctagaaatac aaggatagaa tcgaacaagg gatatgaatc tgctggcgga gtaggtgtca
     2161 cactaggcgg cgttttcaat gcggaggaaa gcgttaccat taaaggcaac cgggctgatt
     2221 ccggtaaccc agaagacttt ccttcttttg gcggcggcgt gttcgtcgcg gaaggcggca
     2281 tcttctatgc caagaacaac acaactatcg aatcaaacga agcggattat ggcggtgggg
     2341 tgtgcgttac cggtccccat gatggtacag aacgaggaaa cttctacgct gaaagcgtca
     2401 caattaaaga gaatcgcgcg aaaaatgtag gaggcgggat cgcgtgggtc accggtattt
     2461 cagagattcg tacccgcgtt aatacttgga cccctgctag cgaccaagaa caggagtttt
     2521 cggtcacgac aaccggggaa attcacgcga aaactaccgg tgaccccgtc caagtctttg
     2581 agaatacgtc ggaaaagggt ggatcgcaga tgaaggtgtt cggcaatcct tagaaaacta
     2641 tagtcgcgaa aaaagaaagg ggcgtggtag gtatgaagag atttttaaag atagtgattc
     2701 tgagttctat aactgcgatg gttttcttgc tatcttcagt gtatgaatgt catggaggag
     2761 atataatagg tgagtcctat ggttctaata ccgttgtgga tttcaggaat tatagtgaga
     2821 aaagaataaa aataatgttt ccgaagaata aacatgagtc gggagaagtt ataattcccg
     2881 ggtgtgaccc taataaaatg ggtccagtct accatcgcat gggtttccag cagggctatt
     2941 tcgatccgcc attgaggttt tttattttta aggaaaatga cgaaggtgat tgggatcctt
     3001 taaataactt gactgtaggt gaatttaaat ccaagggtga cgaagccgtt tctgaaaaaa
     3061 atttgttcag aatatctgat agcgggtata agaagtattc tagacaacac ggtgcatctg
     3121 actggtggca agaggtacct tgatataacc ctaaaaggga tacagctttc ctgagatggc
     3181 atagtataaa aagcgcctca ccactgatga ttcaaaatga ttcgttggtt catcagatag
     3241 gcaataaccg gcaacatgat atctaatagg caatttggcg ctgtaaattc gtatgcggat
     3301 cgacggttca ttccgattcg ttcgataatt gtactggcgc gctatgttta tgcgagcgcc
     3361 tgggaatgcc aattttcaag acggcggttt ttttgggaac gtctgggaac gccgggctta
     3421 agtccggcat gtttttttcc agggaggccc tatcccttga ccggtcttcc tctgtgttct
     3481 ccgagccctc tgtgcgaacc cctgttttta atctttggaa cgaacacctg ggtatcgcgc
     3541 cgtcgcaaag agaagaagcg cttacgccgc gatgccccgc ttgggaaagc ctaccttctc
     3601 cgagccctct gtgcgaattc actcttaagt tctttatcca ggcttatttt ctctgcgttc
     3661 tctctgtgtt ctccgtgttc tctgtgcgag cgagatcttt tgctttaaat ccaggcattc
     3721 tttctctgtg ccctctgtgc gaacccctgt ttttaatctt tggaac
//
LOCUS       DAOPXN010000209         3748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_24856_length_3748_cov_5.137287, whole genome shotgun sequence.
ACCESSION   DAOPXN010000209 DAOPXN010000000
VERSION     DAOPXN010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24856_length_3748_cov_5.137287"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..3414)
                     /gene="drmD"
                     /locus_tag="PKM49_09215"
     CDS             complement(352..3414)
                     /gene="drmD"
                     /locus_tag="PKM49_09215"
                     /inference="COORDINATES: protein motif:HMM:NF038317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HNY83010.1"
                     /translation="MAISEPVEIGRVARARERYWMVTGVDVSDSDTTWVTLESLHDDN
                     LGETLEILRERETHFEILPEEWLHDFDSLDSPEAFDSFLYSLQWQTQSLLPQETIRAP
                     YFGSVSIREYQLEPVIKALASPRIHFLLADDVGLGKTIESGMILQELLLSGKARRILI
                     LCPASLRWQWKWEMEEKFGIDFRIIDRKAIVELRKEYGINVNPWLSFPRLITSYDFLK
                     MEQPLQWFKLTQPVAPPASQSGFVSPFKQWDVLLLDEAHNCLPKPTKHYCQDSDRTRL
                     IREISPMFEHRLFLTATPHNGKREAFIAFLELLDPYRFNRGEPYLDSEAFRKKLDAVM
                     IRRMKRGPDAVKDPFGRPLFPERAIHAIEIVSREEEEEMYTLLNGYLHKRLYSQRVGS
                     HSPSAPEFQSLEFALTILKKRALSSPLAFFRSLEVHLRTGGLPQNIGPQYLNRLNARY
                     QEDQEDDTQKQDLEDALVETLSTGDRGSELSRLYHLTALQKDSADSKTAALLEWMNQT
                     LRQGEQWNTERVVIFTEFLDTLHYLRQQFLSHGIEENRIILLYGGMADEEREAVKRSF
                     ETNPMHHPARILLATDAASEGINLQRYCRYLVHIEIPWNPIRLEQRMGRIDRYGQSRD
                     VHIYHFVYQNREDSRFLRTIVEKTNQIQEDLQNLNPVLSEEIGKRMLGYRTASELPLF
                     VNETLIKERIRAKIEVSVYRQRIEETRRKLSLTPEHIRLVLDNALILLQGEGLRKIEN
                     TGYDRITKLPERWADLTRFFPNNRLPITVAFDEASRDRRDEDAVFLHPSHPLLKRAMA
                     FFRANLWSKRIDTNRNQSQRLNRVTLKAVPSTITSNPLVILYLKGAIQNEFSQVLIEE
                     IVSMGFTFSEGLIVPVDPSFLAGIEHAFIKYKPDPKMGLTMKRLLQENLETLRHTVGE
                     KEKTWTSEYLSQFQEYVKRETRDLESLIKERIREINAAIKPLQKLAQTLLFQEERSQA
                     WEDAQRLLLRKEHLEAELKDIPTRISKKYRVKGAPRLQPIAYCFVLPM"
BASE COUNT          847 a          955 c         1005 g          941 t
ORIGIN      
        1 aaaagtattt cgaaaagctg aagaccctta caatagagaa acttgacgct ctgggccttg
       61 agctaatcga tatgaaaaac gaggaagagt taaagaagta tttaatgtag agagtccggc
      121 aaagggcttg aacacgattt gatgggtcgt gtgtgtgaag caatggagtg cgctcaagaa
      181 acaagaccag aatcggtcgc acgatttcta gtccccgatg actgcaagac caaaaaatca
      241 tactgacgat tctaacatcg tttcttgagt acaccccata acttgttcca tccgcgcacc
      301 acgtttgggc cagtgagcgc gctcccacaa gttcccaagc gctctcgcag gctacatcgg
      361 caggacgaaa cagtacgcga tgggctgcag cctcggcgcc cctttaaccc tgtacttttt
      421 cgaaatgcgg gtcggtatat ccttcaactc ggcttcgagg tgctctttcc tcaacagaag
      481 ccgttgggca tcctcccaag cttgggagcg ctcttcctga aacagaaggg tttgcgcgag
      541 cttttggaga ggtttgatcg cggcgttgat ttcgcgtata cgttctttta tcaaagactc
      601 caggtcgcgc gtttcgcgtt tcacgtattc ttggaattgg cttagatact cgctcgtcca
      661 cgttttttcc ttctcgccga cggtgtggcg aagcgtttcg aggttctctt ggagaaggcg
      721 tttcatcgtg agacccattt taggatcggg tttgtacttt atgaacgcat gttctatccc
      781 cgctaaaaaa gaagggtcta cagggacgat caatccctcg gagaaggtga accccatcga
      841 gacgatctct tcgatcaaca cctgagagaa ttcgttctgg atcgcgcctt taagatacag
      901 aatgaccaag gggtttgacg taatggtcga gggaaccgct ttcagggtga cccggttaag
      961 gcgctgtgac tggttccgat tggtgtcgat gcgtttagac catagattcg cccggaaaaa
     1021 ggccatcgcc cgtttcagga gaggatggct cgggtgtagg aacacggcgt cttcatcccg
     1081 acggtcgcgc gaggcctcgt cgaaggctac ggttatgggt aagcggttgt tcgggaaaaa
     1141 cctcgtcaag tccgcccatc gctctggaag tttggtgatc cggtcgtaac cggtattttc
     1201 tatcttccgc agcccttcgc cctgaagtaa aatcaacgcg ttatcgagca cgagccgtat
     1261 gtgttccgga gtcagtgaga gttttcttct ggtttcctca atccgctgcc gataaacgga
     1321 tacttcgatt ttcgcgcgga tacgctcttt gattagggtt tcattgacga atagcggcag
     1381 ttccgacgcc gttctgtacc ccagcatccg tttcccgatc tcctccgaga ggaccgggtt
     1441 gaggttttga aggtcttcct ggatttgatt cgttttttcg acgatcgtcc gtaaaaaccg
     1501 ggagtcttcg cgattctggt aaacgaagtg gtaaatatga acgtctcggg attgtccgta
     1561 ccggtcgatc cttcccatcc gttgttccaa ccgtatcggg ttccaaggga tttcgatatg
     1621 caccaggtac cggcagtacc gttggagatt gatcccttcc gaagccgcat cggtcgccag
     1681 cagtatccgc gctggatgat gcataggatt ggtttcgaaa gaacgcttca ccgcttctcg
     1741 ctcttcatcc gccatcccgc cgtacaacag gatgatacgg ttttcttcta ttccatggga
     1801 caagaattgt tgccgcagat agtgcagtgt gtccaggaac tcggtgaaga tcacgacccg
     1861 ttctgtgttc cactgctccc cttggcgcag cgtttgattc atccattcca ggagggcggc
     1921 agtttttgaa tcggcgctgt ccttttgaag cgccgtcagg tgatataggc gagagagttc
     1981 cgatccccta tcgcctgttg aaagcgtttc gaccaaagcg tcttctaaat cctgcttttg
     2041 cgtatcatct tcctggtctt cttgataacg cgcgttcaac cgattcaggt attggggccc
     2101 gatgttctga ggaagtccgc cagtccggag gtgcacttct aaagatcgga aaaaagcgag
     2161 gggagaagac aacgcgcgct ttttcaagat ggttaacgcg aattcaaggg actggaactc
     2221 tggtgcgctg ggggaatgcg acccaacgcg ctgagaatac aacctcttat gcaagtagcc
     2281 gtttagaagc gtgtacatct cttcctcttc ttccctggaa acgatttcga tagcgtgtat
     2341 ggcgcgctcc ggaaataggg ggcgcccgaa ggggtcttta acggcgtcgg ggccccgctt
     2401 catccgacgt atcatcaccg cgtccagctt cttccggaaa gcttcggaat cgaggtatgg
     2461 ctctcctcga ttgaaccgat aggggtccag cagctccaga aacgcgatga acgcttcgcg
     2521 tttcccgttg tgcggcgtgg cggtcaaaaa aaggcggtgt tcgaacattg gcgagatctc
     2581 gcggatcaga cgggttctat ccgagtcctg gcagtagtgt ttggtaggtt tcggcaggca
     2641 attgtgcgct tcgtcgagaa gcagcacatc ccactgttta aacggggaga cgaagccgga
     2701 ttgcgatgcg ggaggggcga cgggttgcgt caatttgaac cattgcagcg gctgctccat
     2761 tttcaagaaa tcataagaag tgatgagccg cgggaaagaa agccacgggt tgacgttgat
     2821 tccatactcc ttcctgagtt cgacaatcgc ttttcgatcg ataatccgaa aatcgatacc
     2881 gaatttttct tccatctccc atttccactg ccagcgcaag gacgcggggc agaggataag
     2941 gatacggcgg gctttacccg aaagcaggag ttcctggagg atcattcccg attcgatcgt
     3001 tttccccagc cccacatcgt cggctaacag gaaatggata cggggagaag ccaacgcttt
     3061 gatcaccggt tccagctgat attcccgaat ggacacggaa ccgaaatacg gggcgcgaat
     3121 cgtctcttgc ggaaggagcg actgcgtttg ccactgaagg gaatagagaa aactatcgaa
     3181 cgcctcgggt gaatccaggc tgtcgaaatc gtgtaaccat tcttccggaa ggatttcgaa
     3241 gtgggtctcc cgctctctca ggatttccag ggtttccccg aggttatcgt cgtgcagcga
     3301 ctctagagta acccacgtcg tatcgctatc ggaaacatcc acacccgtaa ccatccagta
     3361 acgctcgcgc gctctcgcga cccgcccgat ttccaccggt tcggaaattg ccatcgaccc
     3421 acctcattca ttttaacaat ttagcaaatc tatggcgatg tcatcattag gattgtatca
     3481 cattctggcc cgtttcgctc gttcccgtgt gggtataata tcggggtaca ccaaattggg
     3541 aacgccgaac tttagttcgg catgtttttt tgcttttcag taagctctcg agagccctta
     3601 aacgcttaca ctgcgatggc cgcgcgaaag cgcatcgagc cgtcgcgaaa ggaagtggct
     3661 cttacgccgc gatggtgcgc ctgggaacgc cggactttag ttcggcatgt tttagaaaag
     3721 taaaagatgg tttcgcacag agaacgca
//
LOCUS       DAOPXN010000210         3733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_24994_length_3733_cov_5.873301, whole genome shotgun sequence.
ACCESSION   DAOPXN010000210 DAOPXN010000000
VERSION     DAOPXN010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24994_length_3733_cov_5.873301"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PKM49_09220"
     CDS             <1..202
                     /locus_tag="PKM49_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83011.1"
                     /translation="LSKKSHRYYTLKAGVSKKKKCLRLFSFSKAYRAVAKSKSAYAAM
                     LPSGTLPMKRQNRLFDTLLEAH"
     gene            932..1360
                     /locus_tag="PKM49_09225"
     CDS             932..1360
                     /locus_tag="PKM49_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNY83012.1"
                     /translation="MVNDLLRKELKTRKFRITAQRELIMKVFSDHFPNHMSIEEVYRN
                     LLEKRSRISKATVYRTVELLSSLGLLRKIPIDEDIVRYELVSNETAGACLAVCTECGK
                     TLRFSEPSIREITALIKKETGFEHLDTHLKVYGVCSDCLK"
     gene            complement(1365..1655)
                     /locus_tag="PKM49_09230"
     CDS             complement(1365..1655)
                     /locus_tag="PKM49_09230"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HNY83013.1"
                     /translation="MNKPVCCKYEVTLRVDGLFSLKSKRSLVGRLKTVLGTRWKVCVC
                     ESHRQDDLKYIGLTIAYLASTRNQAEDILHRLGVALEEESNGIIESSELSTC"
     gene            2237..>3733
                     /locus_tag="PKM49_09235"
     CDS             2237..>3733
                     /locus_tag="PKM49_09235"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNY83014.1"
                     /translation="MTVLDQMMRSVLESLQEGVLIIDSRRSIVYINPEAVRILRLSDE
                     GIIGKDVVKTVPNTRLHIVLQTARPEYDKLQYLGDTVIVTSRLPLFDMAGNLLGVAAI
                     FRDITSVQKMAEEVTNLKEIEAQLKAIIDSTHDAISVADEKGRITLVNEEYTRITGLT
                     SEEVVGKSATIDVIDIDEGDSYHLMVSKTRQPVLGKRLVVGKTRREVIVDVMPLFVGQ
                     SFRGSVGVIHDVTEISSLMHQLVLSRSSVKKSGVRFSFSEIIAVSTKMKIALEQAQQV
                     SSTMATVLLRGESGTGKELFAHAIHHSSPRGEGPFVEINCASLDSSLLQYEIFGYADS
                     TPAASERSTYSGLLKKAQGGTLFLDEISNLDRSTQVKLLGFLQTREYARIGSKTNERP
                     DVRLIVSTNSNLEAMIEEGTFLRDLYFRLNVVPILIPPLRDRLEDLDGLIEIITARFN
                     LEYGKRVRMVTNEVKGLFRQYDWPGNVRELENAIGRAMIKASPSDEVLG"
BASE COUNT          988 a          915 c          950 g          880 t
ORIGIN      
        1 tctcagcaaa aagtcccatc ggtattatac cttaaaggcg ggggtttcca aaaagaaaaa
       61 gtgtctccgc cttttttctt tctcaaaagc gtatcgcgcc gtcgcgaaaa gcaaaagcgc
      121 ttacgccgcg atgctgcctt cgggaacgct cccaatgaaa agacaaaacc ggctcttcga
      181 tacattactt gaagcccact gatcatccaa gaaatacaag gtaacataag aggaatagaa
      241 agagtctgcg ggatggccga ttatgcgatt agagcggatt agcgaaacgg tctaagggtc
      301 tacagaatta ttacgataga agatgttaat taagtacgtc ttgtaaatag tcgaagagcc
      361 acaaaaccat gccaaactaa agtttggcgt tcccagggtg tacccagagc gttacctggg
      421 tgctctctgt gaaaatctta gtgttctctc tgtgttctct gtgctctctg tgcgaacccc
      481 tgtttttttt gacatgccaa cataaaagtt ggcgttccca aggccttccc agggagacag
      541 gccgactgga agtcggcccc acgaagagaa ggggcgctta cgccgcgatg actatatgga
      601 ctaaaaatct gtagatcgat tctggccttg tttcttgatt acactccata acttgacaca
      661 cacgaacgcc tgagttgtag ggcttcggta aggaaggggt acagagtctc aaggcgcgga
      721 aaaaagtaat agttatgtta tccctgcgtt cattcaagtt aaattagtgg taaatttact
      781 taaaatacat aaaatgttac aaaaggttta cttttatctg ctatgatagg gaagatgatg
      841 cccttctaat gacgtgcttt aggattctca agagggctcc gaatagatcc gtttccttta
      901 gaatcgaaga aacggaaatt gggaggtaca catggttaat gatttgcttc gcaaagagct
      961 taaaacgaga aaattcagga ttaccgcgca gagagaattg atcatgaaag tgttttccga
     1021 tcacttcccc aatcatatga gcattgagga agtttatcga aatctactgg agaaacggtc
     1081 tagaatcagt aaagcgacgg tttatcgaac ggtggagttg ttgagcagcc ttggcttact
     1141 ccgaaagatc ccgatagacg aagatatcgt gcgttatgag ttagtctcga atgaaaccgc
     1201 gggcgcttgc ttggccgtat gtaccgaatg cggcaaaaca ctgcgctttt ccgagccctc
     1261 tatacgggag atcaccgcct taatcaagaa agaaacagga tttgagcatc tcgacaccca
     1321 cctcaaagtg tacggcgttt gttcggactg cctgaagtaa actcttaaca ggtcgatagc
     1381 tcactcgatt cgataatccc gttagattct tcctccaacg ccacgcccag gcgatggagg
     1441 atatcttcgg cttggtttct ggtcgatgct aaataggcga ttgtgagacc gatatacttc
     1501 agatcgtcct gacgatggct ctcacaaacg cagaccttcc accgcgtgcc caaaacggtt
     1561 ttcaagcggc cgacgaggct tcgtttgctc ttcagcgaaa aaaggccgtc tactctcaag
     1621 gtgacctcgt atttgcaaca gactggttta ttcatctcgc tcctcgttcg ggaatcccct
     1681 tcctttagcg gtcagggagg gacgtgcgcg gacagtaagg ggtggcgata cgttgaaaat
     1741 tgccgttttc acccacccta ttatactcca caaggggata tgcgtgcgca gatggtgtgg
     1801 gtttaatatc ggggcggaat aagaaaaaag tcaaaaacga ggggggaaag aagtgtattt
     1861 ttcgaccttg cgaaggatta tcgctgggta accgacgggc tgacttccga agcccaaaat
     1921 gtctcgcaat ccgctctgct gttttttctt tttgaaaaac ctgccgaact gaagttcggc
     1981 gttcccaagg cgttcccaga gagacaggcc gactggaagt cgaccccacg aaaagaaggg
     2041 gcgcttacgc cgagatgacc acacggacta aaagccgtac gattgattct ggtcttgttt
     2101 cttgattaca ccccataact tcacacacgc gcgcagaagc ggccctcgtc gagcggatta
     2161 tgctataatc gaaaagtgat gcgcgtcgca cgggttctat ttccgtaacc gcgcaaggcc
     2221 taaaggagac gacgtcgtga cagtgctcga ccagatgatg aggtccgtct tggaatcgct
     2281 tcaagaaggg gttctcatca tagactccag aagaagtatc gtctatatca acccggaagc
     2341 ggttcggatt cttcgccttt ccgatgaagg gatcatagga aaggacgtgg tgaagaccgt
     2401 tccgaacacg cgactgcata tcgtcttgca aaccgctcgc ccggagtatg acaagctcca
     2461 gtacttaggg gatacggtga tcgttacctc ccggttaccc cttttcgata tggctggcaa
     2521 ccttctgggc gtcgctgcga tcttccggga tattaccagc gttcagaaga tggcggaaga
     2581 agtgacgaac ctcaaggaga tcgaggccca gctgaaagcc attatcgact ctacccacga
     2641 cgcgatttcg gtggccgatg agaaagggag gatcaccctc gtcaatgagg aatacacgag
     2701 gattacggga ctcacgtccg aagaggtcgt ggggaagagc gcgacaatcg atgtgatcga
     2761 catcgatgag ggagacagtt atcatctcat ggtctcaaaa acgagacagc cggtattggg
     2821 caaacgcctg gtcgtcggaa aaacccgccg cgaggtcatc gtcgacgtta tgccgctatt
     2881 cgtgggacaa agctttcgag ggagcgtcgg agtgatccac gacgtcactg aaatctcctc
     2941 tttgatgcac cagcttgtcc tatcccggtc atccgtcaaa aaatccggcg tgcggttctc
     3001 tttttctgaa atcatcgccg tgtccacgaa gatgaagatc gccctggaac aggcgcaaca
     3061 ggtttcctcg acgatggcga ccgttctcct acggggggag agcggaaccg ggaaagagct
     3121 gttcgcccac gcgatacacc atagttctcc caggggtgag ggccccttcg tcgaaatcaa
     3181 ttgcgccagt ttggacagct ccctactcca atacgaaata ttcggatacg ctgactcgac
     3241 gcccgcagct tctgaaagat ccacctactc cggccttcta aaaaaagctc agggtgggac
     3301 gctctttttg gatgaaatct ccaacctcga ccgctcgaca caagtcaaac tcctaggatt
     3361 cttgcaaaca cgggaatacg cccgtatcgg ttccaaaacc aacgagcggc cggatgttcg
     3421 tctcatcgtt tccacgaaca gcaatctgga agcgatgatc gaggaaggca ctttcttacg
     3481 tgacctctat ttcaggctga acgtcgtccc tatcctcatc ccgcccttac gagatcgcct
     3541 cgaagacttg gatggtctca tagagatcat tacagcccgc tttaacttgg agtatggaaa
     3601 acgcgtacgg atggtcacga acgaagtgaa gggcctattt cgtcaatacg attggcccgg
     3661 aaacgtccga gaactggaaa acgccatcgg tagggcaatg atcaaggcat ctccctccga
     3721 tgaggtgcta ggg
//
LOCUS       DAOPXN010000211         3709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_25249_length_3709_cov_6.741106, whole genome shotgun sequence.
ACCESSION   DAOPXN010000211 DAOPXN010000000
VERSION     DAOPXN010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25249_length_3709_cov_6.741106"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKM49_09240"
     CDS             <1..628
                     /locus_tag="PKM49_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY83015.1"
                     /translation="SLRPDLVLIAGGFQAAEASRLTPFGLNVMVLNPNSFYDIQRISR
                     FLGALFDLPERGHELAQMMEKTLNRIAVEKAYKIPLTERKTVFFAMVQGAEIKDLWTC
                     AQGSFLSEIIALAGAINITGSYTGPNGWLPIGIEFVALQNPDVILVPYYYEGGANEAI
                     DAILNFAPWKSVSAVKNRAVYAIDGNKASYPNPTLTELLEEIYTTVYE"
     gene            654..1640
                     /locus_tag="PKM49_09245"
     CDS             654..1640
                     /locus_tag="PKM49_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNY83016.1"
                     /translation="MAFIFIGGICFFLFQMSLGTVSIPIDEIIRFFTFRELQTPIYRT
                     ILVDIRFPRLFAGFLIGALLSLSGVFFQALLKNPLADPYILGVSSGGAFGTVLSIAAF
                     GSGTVLSRFFGNLPLVSFLFSLLAAFLTYFLAKRGKSVPVVDLILSGVIMNFFFSAAT
                     TFVIIYGWRNVSSATFWLLGSLSGARWEQVPPLLFIAGVGLLIGFALSKTLNAISLGE
                     EEAKNVGIEVERVKLFLFITGTLVAAYTITITGIVGFVGLIIPHSARMLVGSDHRKSI
                     PLSVFLGGLFFTGCDTIARSLFQPTEMPLGTITSLIGAPLFAVILKRSRRFR"
     gene            1637..2506
                     /locus_tag="PKM49_09250"
     CDS             1637..2506
                     /locus_tag="PKM49_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNY83017.1"
                     /translation="MNRDRPVERDAVISVDGIHFQYDSHPLFHDLSLTVRESEFLSVI
                     GPNGSGKSTLLKLILGIMKPSKGRIAVFGKDIGKMKRKAISQLVSGVLLDFNPAYSFS
                     VEEIVAIGRTPYAKAFSDFTREDRIAIEEALEKTSLLTLRKKPFEHLSSGERQRVLLA
                     KCLAQKTPILLLDECVAHLDPGHVQQFLEVVRDKNRKEKITVVTVFHDINLASLYSDR
                     IVVVDQGKLSQTGDPVEIVHQRLLENVYRAQATVLTHPRFDVPQVLLNRLDRPQKPAP
                     DLIHNELPKSKIL"
     gene            2717..>3709
                     /locus_tag="PKM49_09255"
     CDS             2717..>3709
                     /locus_tag="PKM49_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY83018.1"
                     /translation="MHKITNPHDKFFKEVMSYIEIVRDFLMQYLPPEKARHLDFETLS
                     AEKDSHIGEDLRELFSDLIFRVKLRNEETDAYVLFEHKSAIDKPVGLQLLKYMTAIWG
                     KALSLDPSSPLPIIIPIVFYHGNRKWTISTDFDGLFETGKEHYGAYRRQIPSYEYLLY
                     DFSSTKHEPIRGTQKLRIFLGITRAIFEEEKEVFIETVLDAMKVFDELRGTVGNEEYF
                     EAYIRYLFYARTDLEQEELKERIKTVSMERSEKMLTIAEKLLQEGMEKGLAKGIVKGR
                     EEGLVEGRIEGREEGREEGREELLWKQITKKFPKIPQRYYEKLKALTIDQLDTLG"
BASE COUNT          949 a          927 c          946 g          887 t
ORIGIN      
        1 ctctttgcgg cccgacctcg tcttgatcgc cgggggcttt caagcggcgg aagcctcgcg
       61 gctcactccg ttcggattga acgtgatggt gctgaatccc aactctttct acgatatcca
      121 aaggatctcc cgctttttgg gcgcgttgtt cgaccttccc gaacgggggc atgaattggc
      181 ccagatgatg gaaaaaaccc tgaatcggat agcagtggaa aaggcataca agattccgct
      241 gactgaacga aagaccgtct tcttcgcgat ggtgcagggg gcggagatca aagacctttg
      301 gacctgcgcc caaggctctt ttctcagcga gatcatcgcg ttggcggggg cgatcaacat
      361 caccgggagc tacaccggtc cgaacgggtg gcttccgatt ggaatagaat tcgtcgccct
      421 tcaaaatccc gacgtcattc tcgtgcccta ttactacgaa ggcggcgcga atgaagcgat
      481 cgatgccatt ctgaatttcg ctccttggaa gtctgtcagc gccgttaaaa atagggcggt
      541 gtacgcgatc gacggaaaca aggccagtta tcctaaccct acgcttacgg agctgctgga
      601 agagatatac accaccgtgt atgaatgaat cccgcaggaa gcgaaggctt ctattggcct
      661 tcatttttat aggaggtatc tgcttttttt tattccagat gtccctcgga acggtgagta
      721 taccgataga tgagatcatc cggtttttca cctttcggga gcttcagacc ccgatctatc
      781 ggacgatcct ggtcgacatc cgctttccca ggctcttcgc cggattcctg atcggggcct
      841 tgctctctct ttccggggta ttttttcaag cgttgctgaa aaatccgtta gccgatccgt
      901 atatcctcgg cgtttcttcg ggcggcgctt tcggaacggt tctaagcatc gccgctttcg
      961 gctctgggac tgtcttgtcg cgttttttcg gcaacttgcc cctcgtcagc tttctgttca
     1021 gcttgttagc cgccttctta acctattttc tagcgaagag aggaaagagc gtgcccgtcg
     1081 tggatctcat actgtccggt gttattatga acttcttttt cagcgccgcg acgaccttcg
     1141 tgatcattta cgggtggcgg aatgtgagca gcgcgacctt ttggctttta gggagtctgt
     1201 cgggcgcccg ctgggaacag gttccaccgc ttcttttcat cgccggagtg gggctcctca
     1261 tagggttcgc tctgtcaaaa acgctcaacg ctatctcatt aggagaagaa gaggcaaaga
     1321 acgtcggaat tgaagtcgaa cgagtcaaac tcttcctctt cataacggga acgctggttg
     1381 cagcctatac gatcacgatc accggcatcg ttgggttcgt cggactcatc attcctcact
     1441 cagcgcggat gctcgtcggt tcggatcacc gaaaatccat tcctctctcc gtattcttag
     1501 gcggactctt cttcacaggg tgcgacacga tcgcgcgcag ccttttccag ccgacggaga
     1561 tgcccttggg aacgatcaca tccctgatcg gggcgcccct gttcgccgtc atcctcaagc
     1621 gttcccggag gtttagatga atcgcgaccg gcccgtggag cgagacgcag tcatttcggt
     1681 agatggaatt catttccaat atgactcaca tcccctgttc cacgatcttt ccctaacggt
     1741 acgtgaatct gaatttcttt cggtcatagg cccgaatggc tccggaaaga gcacgcttct
     1801 gaaactgatc ctcggaatta tgaagccatc aaaggggcgg atcgcggtct tcgggaaaga
     1861 catcggcaag atgaaaagga aggcgatctc ccagttggtc tcaggtgtgt tgttggattt
     1921 caacccggcc tattccttca gtgtggaaga aatcgtcgcg atagggagaa caccctatgc
     1981 gaaagccttc tcggatttta cgagagaaga ccgtatagcg atagaagagg cactagaaaa
     2041 aacctcgctt ttaactctgc ggaagaagcc cttcgagcat ctgagcagtg gcgaaaggca
     2101 acgcgtttta ctagcgaaat gtttggctca gaaaaccccg atcttgctgc tggacgaatg
     2161 tgtcgcgcat ttggatcccg ggcacgtgca acaattttta gaagtcgtgc gagacaaaaa
     2221 ccgaaaggaa aagatcaccg tcgtcacggt ctttcacgac atcaacctgg cgagcctgta
     2281 ctctgaccga atcgttgtcg tcgaccaagg gaaactatcc caaacgggcg acccagtgga
     2341 gattgttcac caacgtttgc ttgaaaacgt ctatcgggca caggccaccg ttttgactca
     2401 tccgcgtttc gacgtccccc aagttctgct caaccgattg gatcggcctc aaaaacctgc
     2461 gcccgatctc atccataacg aattaccgaa gtcgaaaatc ctgtgacgct ctaccgatac
     2521 gacggaccgt gggtgtgtgt gaagttatga agtgtaatca agaaacgaga ccagaattga
     2581 tcgtacgatc tacgttctct ccgtaccctc tgtgccaaac cttcttttat ctttttacac
     2641 tcccggaaaa tcaaaactcc cctctttatt agtgtataat acccgtgaac gagttttctc
     2701 tatagggagg agcccaatgc acaagatcac aaatcctcac gataagttct tcaaagaagt
     2761 gatgagctat atagaaatcg taagagattt tttgatgcag tacctgcctc ccgagaaagc
     2821 aaggcacctt gatttcgaaa cgctctcagc agaaaaagac agccacatag gcgaagactt
     2881 acgggagctc ttttctgacc tcatattccg agtgaagctc cgaaacgaag aaacggacgc
     2941 ctacgtgttg ttcgagcaca aaagcgcgat tgataaaccc gtaggactac aactcttgaa
     3001 atatatgacg gcgatatggg gaaaggctct gtcgctcgat ccatcgtctc cgctaccgat
     3061 catcataccg atcgttttct atcacggaaa caggaagtgg acgatctcca cagattttga
     3121 cggattattc gaaactggga aagaacacta tggggcgtat agacgccaga ttccgagcta
     3181 tgaatatttg ctgtatgatt tttcgtcaac gaaacatgag ccgatacgag ggacgcagaa
     3241 gctacggata ttcctcggga tcacccgagc gatcttcgaa gaagaaaagg aagtattcat
     3301 agaaacggta ctcgacgcga tgaaagtctt cgacgaatta aggggcacgg tagggaacga
     3361 agaatacttc gaagcgtaca tccgatatct attttacgct cggacggact tggagcaaga
     3421 ggagctcaag gagcggataa agacggtatc gatggaaagg agtgaaaaga tgctaacgat
     3481 tgcggaaaag ctacttcaag aaggtatgga aaagggatta gcaaaaggga tagtaaaagg
     3541 ccgagaagaa ggtctggtag aaggtcggat agaaggcaga gaggaaggtc gagaagaagg
     3601 ccgagaagaa cttctttgga aacagatcac aaaaaaattc ccaaaaatcc cccaaaggta
     3661 ctacgaaaag ctgaaagcgc tcacgataga ccaactcgat accttgggc
//
LOCUS       DAOPXN010000212         3690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_25401_length_3690_cov_4.142779, whole genome shotgun sequence.
ACCESSION   DAOPXN010000212 DAOPXN010000000
VERSION     DAOPXN010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25401_length_3690_cov_4.142779"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1207
                     /locus_tag="PKM49_09260"
     CDS             89..1207
                     /locus_tag="PKM49_09260"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNY83019.1"
                     /translation="MNQLPELRIGDNVIPLPIIQGGMGVGVSLSGLASAVANEGGIGV
                     IASAGIGTFHFDWDQGTMSLMDLDREGLRLEIRKARSLTKGWIGVNIMAALNDFSSLL
                     RVALEEGADFIFIGAGLPFWDTNEIPLEVIHRTKTKLVPIVSSAKAARLIFQYWDKKY
                     GRVPDALVLEGPKAGGHLGFSLRQINNPDYQLENLIPQLVEQVKELETDYARQIPIIA
                     GGGIFTGGDIRKALEMGVQGVQMSTRFVGTFECDASNRFKEAYLECQPEDITIIKSPV
                     GLPGRALRNQFIIDVESGLKKPFVCPYRCLSTCDFVNAPYCIAMALTNAQKGILEEGF
                     VFCGANAYRVKKIVSVKELVQTIKEEYQASFENVPDTR"
     gene            complement(1215..2606)
                     /gene="dnaB"
                     /locus_tag="PKM49_09265"
     CDS             complement(1215..2606)
                     /gene="dnaB"
                     /locus_tag="PKM49_09265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNY83020.1"
                     /translation="MAEGVRQVRTPPNDAEAERAVLGSMLIDPETVGDVMEVITSEDF
                     YNEKHKIVFRAMETLVEKADPVDVVTLSDVLRKEAAIEKIGGEIFLVQLAEAVPTSAH
                     CVEYAKLVREKSILRNLINTGSKIVENAYSLEEVDKILDQAERSILKIGERTLDRSFR
                     PLKAIIDKAFENLVDIKVKHDSRQDNKYVSGVSTGYDALDMLTTGFHHSDLVIVAARP
                     SMGKSALALNFAYNMSLQTNQMVGVFSLEMSAEQIALRLLSVASRVPFSKLRKGDITN
                     DEWDRLTNAASRLTDANLIIDDDSMLEPRTLRTKARRIKKEYGLDIVVVDYLQLMNCG
                     KERENRQQEISEISRSMKLLGKELDVVVIAASQLSRSIEQREDKRPRLSDLRESGAIE
                     QDADTVLFIHREAYYKRKKDGSDENEVQIMNEPHEAEIIIGKQRNGPIGTVRLTFDPN
                     YVSFYPVDYGHLG"
     gene            complement(2623..3504)
                     /gene="lgt"
                     /locus_tag="PKM49_09270"
     CDS             complement(2623..3504)
                     /gene="lgt"
                     /locus_tag="PKM49_09270"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNY83021.1"
                     /translation="MKKWIVAIIAIVALVLLFFFVKGVFDNSIQLNPVAFKIGPFYIH
                     WYGIVIAAGIILCYIIGSRIGVKQGMNEEHMAEAIIVCIFFGIAGARLYYVFSQWDLY
                     KNNLLEIFMTWHGGLAIYGGVLGALLGGALYTRFRKNCSFTLFQGTDIAAAMLPLGQA
                     IGRWGNFFNYEAYGSPTDLPWKMFIPFPYRMPGYEGFEYFHPTFLYESAWDILTFCVL
                     FFFYAKKRKNHGETTALYFIVYSLGRIFVESLRLDSLYWGEWRAAQVTGVILMAVGIL
                     LFVYFRKKGKPIVKQTA"
     gene            complement(3501..>3690)
                     /locus_tag="PKM49_09275"
     CDS             complement(3501..>3690)
                     /locus_tag="PKM49_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNY83022.1"
                     /translation="KVNINLIPVNPGAEPYRRPSEEKVARFQSILTKNGFEAVQRVEK
                     GADIEGACGQLRNRGDKR"
BASE COUNT          866 a          968 c          945 g          911 t
ORIGIN      
        1 ctgtttcacg ccttggccga tttcgtgtat aatcggcagg aaatagaagg catcgttacg
       61 gaggattatc gatgacaagg agttggatat gaatcaatta ccggagttgc gaatcggaga
      121 caacgtgatc ccgcttccca tcatacaggg cggaatgggt gtcggggtat cgttatcggg
      181 tttggcgagc gctgtcgcga atgaaggcgg catcggcgtg attgcaagcg ctggaatcgg
      241 caccttccat ttcgattggg atcagggcac gatgtccctg atggacctgg atcgggaagg
      301 attgcgtttg gagattcgga aggcgcgatc tttaactaaa ggatggatcg gcgtgaacat
      361 catggcggct ctgaatgatt tttcatcgtt gctgcgtgtc gcgctggaag agggagccga
      421 ttttattttt atcggagcgg ggttgccttt ttgggatacg aatgaaatcc ctttagaggt
      481 gatccaccgg acaaaaacga aactcgtgcc aatcgtctct tccgcgaaag cggcgcgtct
      541 gatcttccaa tactgggata aaaaatacgg gcgtgttccg gacgcattgg ttctcgaagg
      601 gccgaaagcc ggcgggcact taggtttttc tttgaggcag atcaataatc ccgattatca
      661 actggaaaac ttgattcccc agttagtgga gcaagttaaa gagttggaaa ccgattacgc
      721 ccgccaaatt cctatcatcg ccggaggcgg gatcttcacc ggcggagata ttcgaaaggc
      781 tttggagatg ggtgtgcagg gagtccaaat gagcacgcgg ttcgtcggta ccttcgaatg
      841 cgacgcttcc aaccgtttca aagaagcgta ccttgaatgc caaccagagg acatcaccat
      901 catcaaaagc ccggtggggc ttccgggacg agcgttgcgc aatcagttca ttatcgatgt
      961 ggaaagcggt ttgaagaagc ctttcgtatg tccctatcgc tgcttgagca cttgcgactt
     1021 cgtcaacgcg ccttactgta tcgcgatggc cttaacgaac gcccaaaaag ggatcttgga
     1081 agaggggttc gtcttttgcg gcgcgaatgc ctaccgcgtg aaaaagatcg tgtcggtcaa
     1141 ggagcttgtc caaacaatta aagaggagta tcaagcctct ttcgaaaacg tgccggatac
     1201 gcgctaaggc tatatcatcc caaatgcccg tagtcgacgg gatagaaaga gacgtagttt
     1261 ggatcgaagg ttaatcgaac ggtcccgatg gggccgtttc tctgtttacc gatgatgatt
     1321 tcggcttcgt ggggctcgtt catgatctga acctcgttct cgtccgagcc gtctttcttt
     1381 ctcttgtaat acgcttcccg atgaatgaac agaacggtat ccgcgtcctg ttcgatagcg
     1441 ccggattccc tgagatctga gaggcgggga cgtttgtcct cgcgttgctc aatagatctt
     1501 gagagttgag acgccgcgat cacgacgaca tcgagttctt tcccgaggag tttcatcgat
     1561 cgcgagatct ctgaaatctc ctgttgcctg ttctctcgct ctttcccgca attcatcagt
     1621 tgaaggtagt caacgaccac gatgtccagc ccgtactctt ttttgattcg ccgcgccttc
     1681 gtccgcaggg ttcgcggttc cagcatcgaa tcgtcgtcga tgataaggtt ggcgtccgtc
     1741 aaccgacttg ccgcgttcgt caagcgatcc cattcgtcgt tggtgatgtc tccttttctc
     1801 aatttggaaa agggcacgcg ggaggcaacg ctcaacaaac gcagcgcgat ctgctcggct
     1861 gacatttcca ggctgaagac gcccaccatc tgattggtct gcaaagacat gttgtaggcg
     1921 aaattcagcg ccaaagccga ttttcccatc gaaggacgag cggcgacgat caccagatcg
     1981 gaatgatgga agcccgtcgt cagcatatcc aacgcatcgt atccggtgga aacccccgat
     2041 acgtacttgt tgtcctgccg gctgtcgtgt ttgaccttaa tatcaacgag gttttcgaag
     2101 gctttgtcga taatggcttt cagtgggcgg aaagaacgat ccaacgttct ttccccaatt
     2161 ttcaagatcg agcgctccgc ctggtccaaa attttatcga cctcttcgag gctgtaagcg
     2221 ttttcgacaa tcttgctgcc agtattgatc agattccgta agatggattt ctctcgtacc
     2281 agcttcgcat actccacaca gtgagcggag gtcggtacgg cctccgctaa ctgaaccagg
     2341 aatatttccc cgccgatctt ttcgatcgcc gcttctttgc gtaaaacgtc ggagagtgtg
     2401 acgacatcga cgggatctgc tttttccacc aaagtctcca tcgcgcggaa aacgattttg
     2461 tgtttttcgt tgtaaaaatc ttcgctcgtg atgacttcca tcacgtctcc caccgtttcc
     2521 gggtcgatca gcatcgatcc tagaacggcg cgttccgcct ccgcgtcatt cggcggcgtt
     2581 cgtacctgtc taaccccttc ggccatggac ggttccggca gcctaagcgg tttgtttcac
     2641 aatcggtttg ccttttttgc ggaagtagac gaacaacaaa ataccgactg ccatcagaat
     2701 gacaccggtg acctgcgccg cgcgccactc tccccagtaa aggctgtcca atcgcaatga
     2761 ctctacgaag atgcgtccga gagaataaac gataaagtag agggcggttg tctcgccatg
     2821 gttcttcctc ttctttgcat aaaagaagaa gaggacgcaa aacgtcagga tatcccaagc
     2881 gctctcgtat aaaaaagtgg gatggaaata ctcgaatcct tcataacccg gcatacggta
     2941 gggaaacggg atgaacattt tccacggcaa atcggtcggg ctgccgtacg cctcatagtt
     3001 gaagaagttg ccccatcttc cgatcgcttg ccccagaggg agcatcgccg ccgcgatatc
     3061 agttccctga aagagcgtga aggagcagtt tttcctaaaa cgcgtgtaga gagccccgcc
     3121 aaggagcgcg cccagcacac ctccgtatat cgccaaccct ccgtgccaag tcataaagat
     3181 ttcaagaaga ttgtttttat acaaatccca ttgggagaac acataataaa gccgcgcgcc
     3241 ggcaatccca aagaagatgc acacgataat ggcttccgcc atatgctcct cattcatccc
     3301 ctgcttcacg ccgatgcggc ttccgatgat atagcacagt atgatccccg cggcaataac
     3361 gatcccgtac cagtgaatat agaacggccc gattttgaac gcgacaggat tcagctggat
     3421 cgaattgtcg aagacccctt tcacaaagaa aaagagcaac accaaagcaa cgatggcgat
     3481 aatggccaca atccactttt tcatctttta tcccccctat ttcttaattg cccgcaagcc
     3541 ccctcgatat ccgcgccttt ctccacgcgt tggacggctt caaacccatt tttcgtcaga
     3601 attgactgga aacgcgccac cttctcttcg cttggccgcc ggtagggttc ggcccccggg
     3661 ttcaccggta tcaggttgat attgaccttc
//
LOCUS       DAOPXN010000213         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_25425_length_3688_cov_5.011285, whole genome shotgun sequence.
ACCESSION   DAOPXN010000213 DAOPXN010000000
VERSION     DAOPXN010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25425_length_3688_cov_5.011285"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PKM49_09280"
     CDS             <1..261
                     /locus_tag="PKM49_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY83023.1"
                     /translation="LEKMSHFSPDIMILDLMMPKLDGFGVLREKAKMEGFKDTPTIVL
                     TAKGGEDDRENALSLGAIDVLTKPFSPKQLLDCVKKVVGDAV"
     gene            251..1711
                     /locus_tag="PKM49_09285"
     CDS             251..1711
                     /locus_tag="PKM49_09285"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNY83024.1"
                     /translation="MPYRKALASVYHKLCELLGDPIDPTIEGADEEVLFKRIESQFAS
                     LQSEFVSYKDQLEESSIMLETQVEEISKTYEEISTLYGVTSLLSKTLNPTEVMGDLME
                     LVLNSVPSGFISIYLAMGTEGTEEYYKYVEKEGRIVRSLADGLKGYFRNLIQQNQAKV
                     IILEEEGIRKRFPNCVDRMHSAMIVPLGTSQKLWGFLALANKQNGNLYTAGDRKLLES
                     ISNQIFFGLENYQFLQEQIKQERFFEQLEIAKKIQESLLPQKIPQLRRLDIACHFEPA
                     VEVAGDYYDIIHSGDKVFLVIADVSGKGVPASLLMSSFRSTVRIFLSVTEELPRLVEK
                     VNDHIAQNEIADRFITGLFVNLDLRTGQIKYVNAGHDPMLLYRRSEDRFFEITNDGIP
                     TGIFEGESYLESEFTLLPGDLFILYTDGIPEARNGAKEEFGFERMQEVLRVNCEKTAE
                     EISSAMTEALGRFVDSAPQHDDITFMILKYRGNLAE"
     gene            1729..2490
                     /locus_tag="PKM49_09290"
     CDS             1729..2490
                     /locus_tag="PKM49_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012580086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNY83025.1"
                     /translation="MSGHNKWANIKHRKGAQDKKRSTLFTKLIRELTIAAKEGGGDAD
                     SNPRLRAAVDKAREGNMPKDTIEKAIKRGTGELEGVEYVEVMYEGYGPAGVAMLVYTT
                     TDNKNRTAQEVRHVFSKYGGNLAESGAVSWTFEKKGRFFIEQSEISDMDQFVLESIDF
                     GADDVDDSSDPVTLFTLPENLMRLADTLTEKGLHPKEIEFTYEPKNRVAVTGKDAEKV
                     MLLISKLEDLDDVQNVFANFDIADEELEAIMAEID"
     gene            2515..3429
                     /locus_tag="PKM49_09295"
     CDS             2515..3429
                     /locus_tag="PKM49_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNY83026.1"
                     /translation="MFSLRAGQRSIDQKIAIDLHLHTHFSDGRHSARELFQMLRSESV
                     WLCSVTDHDTIEHIPEMRAEAKAMGLVLLPGVEISTFFEGGEVHILGYGYSLDDSSFS
                     LFLRERFANRTGRAREMVALLNRVGVEISYEDVQKQSPGPYVGRPNIARAMKSKGYIK
                     ELSEAFSRTYIGVGGRAYVPPSECHPREAIEAIRKAGGAAVLAHPGLYYAEEGCRGET
                     SQKGLNQNQIAQMRAYGLEGIECYHSKHSFSDSQRYAQICKQLDCIRTMGSDFHEGAY
                     FPIHWQVPKPVLEETCRWLRDRFSCSLP"
     gene            3393..>3688
                     /locus_tag="PKM49_09300"
     CDS             3393..>3688
                     /locus_tag="PKM49_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83027.1"
                     /translation="MVKRPILLFLALIFAFPSACLFGAEVAQDDFEALDWTLPFPDVI
                     LQNMAASITFGMGPFINPAGAGSVVGLYAQIRPTLRFLIFDGAFNVGFRPYSQD"
BASE COUNT          934 a          855 c         1049 g          850 t
ORIGIN      
        1 ttagaaaaga tgagccattt ctctcccgat atcatgatct tggatctcat gatgccgaaa
       61 ctggacggtt tcggcgtttt gagagaaaag gcgaagatgg agggcttcaa ggacacaccg
      121 accattgtgc tgacggcgaa gggcggagaa gacgatcggg agaatgccct cagtttaggc
      181 gcaatcgatg tgttgactaa accattcagt ccgaagcagc ttttggattg tgtcaaaaag
      241 gtggtggggg atgccgtata gaaaagcctt ggcatccgtt tatcataaac tgtgcgagct
      301 cctgggcgat ccgatagacc ccacgatcga aggcgccgat gaagaggtgc tttttaaaag
      361 gatcgagtcc cagttcgcct ctttacaaag cgagttcgta tcctataaag accagttgga
      421 ggagtcctcg attatgttgg aaacccaagt ggaggagatt tccaagacct atgaggagat
      481 ctcgacactc tatggcgtga cctcgctact gtccaaaacc ctaaatccta ccgaggttat
      541 gggcgacctc atggaactgg ttctcaactc tgtgccctca gggttcatca gtatctatct
      601 ggcgatgggg accgagggga ccgaagagta ttataaatac gtcgaaaaag agggaaggat
      661 cgttcggtct ctcgccgacg gactgaaagg gtatttcaga aatctgatcc agcagaacca
      721 agccaaagtg attattttgg aggaagaggg cattcggaag cgatttccga actgtgtcga
      781 tcgtatgcac tcggcgatga tcgtcccgtt gggaacgagt cagaagcttt gggggttcct
      841 tgctctagcg aacaagcaaa acggaaacct atatacagcc ggagaccgta aactgttgga
      901 atctatcagt aaccaaatct tcttcggttt ggagaactat caattccttc aagagcagat
      961 caagcaggaa cgattttttg agcagttaga gatcgcgaaa aagatacaag aaagcctgct
     1021 gccacagaag attcctcagt tgcggagatt ggacatcgcg tgtcatttcg agccggctgt
     1081 ggaagttgcg ggcgattact atgatatcat ccactcggga gacaaggtat ttttggtcat
     1141 tgcggatgtt tccgggaaag gcgttcccgc gtccctattg atgagttctt tccgatccac
     1201 cgtccgtatc ttcctatccg tcacggagga gctgccccgt ttggtggaga aagtgaatga
     1261 ccacatcgct cagaacgaga tcgccgaccg gttcatcact ggactgttcg tcaacctgga
     1321 tttgagaacg gggcaaatca aatacgtcaa cgcggggcac gatccgatgt tgctttaccg
     1381 ccggtccgaa gaccgttttt tcgagatcac caacgatggg atccctacgg gcattttcga
     1441 aggggagagt tacctggaga gcgagtttac gctgttgccc ggcgacctct tcatcctata
     1501 caccgacggc ataccggagg cgcgcaacgg tgctaaagaa gagttcggct tcgagcgtat
     1561 gcaggaagta ttgcgtgtga actgcgaaaa gacggccgag gagatcagct cggcgatgac
     1621 ggaggcactc gggcgttttg tggattcggc gccgcagcat gatgatatta cgttcatgat
     1681 actcaagtac cgaggaaatc tcgctgagta gaataaagga gggttattgt gtcgggccat
     1741 aataagtggg cgaatattaa acaccgaaaa ggtgcgcaag acaagaaacg gtcgacgtta
     1801 ttcacgaaac tcatcaggga actcaccatc gccgcgaaag aaggcggcgg cgacgcggat
     1861 tcgaatccca gattacgggc ggccgtggac aaagcccgag aagggaacat gcctaaagac
     1921 accattgaaa aggccattaa aagaggtacc ggagaattgg agggggtcga atacgtcgaa
     1981 gtgatgtacg aagggtatgg ccctgcgggt gtggctatgt tggtgtacac gaccaccgac
     2041 aacaaaaaca ggacagcaca ggaagtgcgg catgtcttca gcaagtacgg ggggaatctg
     2101 gccgagagcg gcgcagtcag ctggaccttc gagaagaagg ggcgattttt catcgagcaa
     2161 tcggaaatct ccgatatgga ccagttcgta ttggaatcga tcgattttgg agccgacgat
     2221 gtggacgact cctcggatcc agtgaccttg ttcaccttac cggaaaactt gatgcgactg
     2281 gccgataccc taacggagaa agggctccat cccaaggaga tcgagttcac ctacgagccg
     2341 aaaaacaggg tcgccgtcac cggaaaagac gcggaaaagg tgatgttgct gatctccaaa
     2401 ctagaagacc tcgatgacgt gcaaaacgtt ttcgcaaact ttgatattgc agatgaagag
     2461 ctcgaagcga ttatggcgga gattgactga tttggtagga tttgtgtttc gtcgatgttc
     2521 tctttgagag ccggccaacg gtcaatcgac cagaaaatcg cgatcgattt acaccttcat
     2581 actcattttt cggacggtcg acacagcgcg cgagagcttt ttcagatgtt gcgctcggaa
     2641 agcgtgtggc tctgttctgt gacggaccac gacaccatcg aacacattcc ggagatgcga
     2701 gcggaagcca aggcgatggg gttggtcttg ctgccgggcg tggagatttc gacctttttt
     2761 gaaggaggag aggtccatat cctggggtac ggctactcct tggacgattc ctctttttcc
     2821 ctttttttac gagagcggtt tgcgaaccgt accggacggg cgcgagagat ggtcgcactc
     2881 ttgaatcgtg tcggtgtgga gatttcttac gaagacgtac aaaagcaatc gccaggaccg
     2941 tacgtcggtc gcccgaatat cgcccgggca atgaagagca aaggctatat aaaagagctt
     3001 tcggaagctt tttctcgaac gtacataggc gttggcggac gagcttacgt gccgccttct
     3061 gaatgtcatc cgcgggaagc gatcgaggcc attcgcaagg cagggggtgc ggctgtgttg
     3121 gctcatccag gcttgtatta cgcggaagag ggatgtcgag gagaaacctc gcaaaagggt
     3181 ttgaaccaga atcaaatcgc tcagatgcgc gcctacggtt tggaaggaat cgagtgctat
     3241 cactccaaac atagcttctc cgacagccaa agatacgcgc aaatatgcaa acaattggat
     3301 tgtatacgaa cgatgggctc cgattttcac gagggcgcct attttcccat acactggcag
     3361 gttcctaaac ccgtcttgga ggaaacgtgt cgatggttaa gagaccgatt ctcttgttcc
     3421 ttgccttgat ctttgcgttt ccgtcggcgt gtttgtttgg cgccgaagtc gcgcaagacg
     3481 atttcgaggc cctggactgg acgcttccat ttccggacgt gattttgcaa aatatggcag
     3541 ccagcattac ctttgggatg ggtccattca tcaatccagc cggcgctggg tcggtcgtgg
     3601 ggctttacgc gcagatcaga cccaccttac gctttctcat ctttgacggt gccttcaacg
     3661 tcgggttcag gccgtattct caggatca
//
LOCUS       DAOPXN010000214         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_25638_length_3665_cov_5.089197, whole genome shotgun sequence.
ACCESSION   DAOPXN010000214 DAOPXN010000000
VERSION     DAOPXN010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25638_length_3665_cov_5.089197"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /gene="hfq"
                     /locus_tag="PKM49_09305"
     CDS             complement(<1..275)
                     /gene="hfq"
                     /locus_tag="PKM49_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNY83028.1"
                     /translation="MAEKFNLQDRFLNHLRLHKVEAKVFLVNGFQIRGEIRSFDSFTI
                     LLEDGKTQSLIYKHAISTITPSSYVKLAKPQEDSSSEEESSKPASLS"
     gene            complement(383..1306)
                     /gene="miaA"
                     /locus_tag="PKM49_09310"
     CDS             complement(383..1306)
                     /gene="miaA"
                     /locus_tag="PKM49_09310"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNY83029.1"
                     /translation="MKIPVLFGPTAVGKTDLVLKLCETYPIEVISMDSMQIYRQMDIG
                     TAKPSKEVLAVAKHWMIDILDPQEEYNAYRYRSDALKAIEDVRSRDKIPLVVGGTGLY
                     LDALIHGLFEGVSKDEGLRRELRKTEENNPGYLRAFLKEADPESYARIHPNDIKRTIR
                     ALEVYLLSGKTLSTAQKDRVGDERFLIVECVRGREELYSRIDARVDAMIQEGLLEETR
                     ALLEQGCTAQTQSMKAIGYRESIQFLQGLIPTKEQYRHRLKVNTHHYARRQIIWGRKY
                     ARKTSCDLSTERFGEKEQKGPIIIERLLEML"
     gene            complement(1303..2115)
                     /locus_tag="PKM49_09315"
     CDS             complement(1303..2115)
                     /locus_tag="PKM49_09315"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY83030.1"
                     /translation="MGCNDSLIAGGLADSKNFYVTTSDKPNRRQVDKAKRLAEELSAP
                     YLDKRRSILANRESHHDFYYVVERNRVLIRRQSDIFFFHPSSAKMRMRNWNHGQNEYL
                     LDAFQLSGGERILDLTLGLGSDAILMGGFLREGQVIGLESSLHIYTIVRYGLQDYVDP
                     ADWINESMKRITPIHADYKTYLRTTPDKSFDLVYCDPMFDHPVMRSSGLNPIRPFANH
                     EGVSEEDIEEMRRVSRGRVVFKTLIRDRLLGQIPVNRVYGSKSSGILYGVIL"
     gene            complement(2102..2302)
                     /locus_tag="PKM49_09320"
     CDS             complement(2102..2302)
                     /locus_tag="PKM49_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83031.1"
                     /translation="MIYWFTILALSSWMVYLNIHVVFEYQGASAKYETVLREYEAKKK
                     ELEEKMEEYLRLRARMEEHGLQ"
     gene            complement(2397..3386)
                     /locus_tag="PKM49_09325"
     CDS             complement(2397..3386)
                     /locus_tag="PKM49_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83032.1"
                     /translation="MRKLRRALFFGILGALLPVFLTSCFVLNIFFPLPGKAKRNLSVF
                     LHYKNLSLENHEMVSVELYLTKVEFGGKSLDVKKAFVLSIAPARQTQNLTDFFSNEHI
                     VLGQYTFSETEANISKPSFTFTCGNTATITYHEYVASEFGGNGTVEATAIAFNLANTT
                     FEAPVMQLSSARFDKYASLTLPGGQSRLILMLNLKSIPTLQALKGGSDRSFSLSGSIS
                     MVSRDLCFVYGSVSDLSSAGTLVPGQYWTVDFDQTSGLVFEDYYWGFGTYTQDESLKS
                     HRYFFFAPQITNQSYPAVFELYLPGHTSSVGRKEFEVPDVDELRVDMTIQSDN"
     gene            complement(3409..>3665)
                     /locus_tag="PKM49_09330"
     CDS             complement(3409..>3665)
                     /locus_tag="PKM49_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HNY83033.1"
                     /translation="ILVCGTGIGMSIAANKFPGIRAALACYPDMAKLARAHNNANVIA
                     LGGRVMGEELAIWCVESFIHSEFDGGRHQLRVDKLDRFIK"
BASE COUNT          851 a          958 c          899 g          957 t
ORIGIN      
        1 ctagaaagag acgccggttt gctggattcc tcttcggaag agctatcttc ttgcggtttg
       61 gccaatttca cataagacga aggagtgatc gtgctgatcg cgtgcttata gatcaagctc
      121 tgggtcttgc cgtcctccag aaggatcgta aaactgtcga acgatcgtat ttcccctctg
      181 atttgaaacc cgttgactag gaacactttc gcttcgactt tatgtaagcg caagtgattc
      241 aagaaccgat cctgtaaatt gaatttttct gccatatcca cccctgccct gtcattagaa
      301 tgaggagaac acacctcgta aaagaaaacc gcttgtttaa caagcgccgg atgcctaccg
      361 aatacctgcc gtacgagaaa gattataaca tttccaagag tctttcgata atgatagggc
      421 ctttttgttc tttctctcca aaacgctcag tggataagtc gcacgaggtt tttcgggcgt
      481 acttcctgcc ccagatgatc tggcgtcggg cgtagtggtg ggtattgacc ttcaatcgat
      541 gtcggtattg ttcctttgtg gggataaggc cttgtaaaaa ctgtatggat tcgcggtatc
      601 cgatcgcctt catcgattgc gtttgggcgg tgcagccctg ctccagcaat gcgcgagtct
      661 cctccaacaa cccttcttgg atcatcgcat cgactctggc atcgatacgc gaatacagtt
      721 cttcgcgtcc gcgaacgcat tcgacgatca agaaccgttc gtctccaacc cgatcttttt
      781 gagcggtgga gagcgttttt ccgcttaaga ggtagacttc gagcgcccgg atcgttcttt
      841 taatgtcatt cgggtgtata cgcgcgtagc tttccggatc cgcttctttc aggaaagccc
      901 gtaaataacc ggggttgttt tcttcggtct ttctcaactc ccgccgcaaa ccctcgtctt
      961 tcgagacgcc ttcgaataga ccgtggatca gcgcatcaag gtataatcca gttccgccta
     1021 ccactagcgg aattttgtcg cgcgagcgga cgtcttcgat cgccttcagc gcatcggagc
     1081 gataccgata cgcgttgtat tcctcctgag ggtccaaaat atcgatcatc caatgcttcg
     1141 caaccgccaa gacctctttc gagggtttgg cggttccgat atccatctgt cgatagattt
     1201 gcatcgaatc catcgaaatg acctcgatgg gataggtctc gcacaatttc agaaccaaat
     1261 cggttttgcc caccgcggtg ggcccgaaaa gtaccggtat cttcatagaa taaccccata
     1321 aagtatgcca ctgctcttgc tgccgtacac tcgattcacc ggtatctgcc cgagcaatcg
     1381 gtctcgtatg agcgtcttaa acacaacgcg acctcgcgac actcttctca tctcttcgat
     1441 gtcttcctcc gacactcctt cgtggtttgc gaaagggcgt ataggattca atccggacga
     1501 gcgcatcacg ggatggtcaa acatcgggtc gcagtaaacg agatcgaaag acttatccgg
     1561 agtcgttctg agataagttt tatagtctgc gtggatcggt gtgattcgct tcatactctc
     1621 attgatccag tcggccggat ccacgtagtc ttgcaaaccg taccgaacga tcgtgtaaat
     1681 atgcaacgaa gactccaacc cgataacctg tccttctcgt agaaagccgc ccatcagtat
     1741 tgcgtcggac cctaaaccga gggtcaagtc taagattcgc tccccgccgg agagttggaa
     1801 ggcgtctagc aggtattcgt tctgtccgtg gttccaatta cgcatccgca tcttcgcgct
     1861 ggatgggtga aagaagaaaa tgtcagactg tcggcgtatc aggacacgat ttctctccac
     1921 gacgtagtaa aaatcgtgat gactttctcg attggccaag atcgaacgtc gcttatcgag
     1981 gtaaggtgcg gaaagctctt ccgccagccg ttttgcctta tcaacttggc gccggtttgg
     2041 cttgtccgaa gtcgtgacgt agaagttttt cgaatccgcc aaccccccgg ctattaagga
     2101 gtcattgcaa cccatgttct tccattcggg cacgtaagcg taaatactct tccatctttt
     2161 cctctaactc tttctttttc gcttcgtact cccgcaggac tgtctcgtac ttcgcgctgg
     2221 ctccctggta ttcgaacacg acgtggatgt ttaggtagac catccagctg ctcaacgcca
     2281 gaatcgtgaa ccaatatatc aaggagatct tccttttctt ttttttctgc ggagtgtttt
     2341 tctttgggga caggttccca agattatttc cggaagaggg ttttgggtaa agcatctcaa
     2401 ttatccgact gtattgtcat atccacgcgc aattcgtcga cgtccggcac ttcgaactct
     2461 ttacgaccca cactgctggt atgcccggga aggtacagtt cgaaaacggc tggataggac
     2521 tggttcgtga tttgcggagc gaagaaaaaa tatcgatgac tcttgagcga ctcgtcctgc
     2581 gtgtaggtcc caaagcccca ataatagtct tcaaacacga ggcctgaggt ctgatcgaag
     2641 tcgacagtcc aatactgtcc cggaaccaag gtccctgccg aggagagatc cgaaacggag
     2701 ccatagacga aacagaggtc cctggaaacc atcgaaatag atccgctcaa agaaaacgag
     2761 cgatcgcttc cgcctttcag cgcttggaga gtcgggatcg atttcaagtt tagcatcaga
     2821 atcaggcggc tttgcccacc cggcagcgtg aggctggcgt atttgtcgaa ccttgcgcta
     2881 gaaagttgca taacgggcgc ctcaaacgtg gtattggcta aattgaaggc aatagcggtg
     2941 gcttcgacgg tcccatttcc gccaaactcg ctcgctacat actcgtgata ggttatggtc
     3001 gccgtgtttc cgcaggtaaa agtgaatgaa ggtttggaga tattagcctc tgtttcggaa
     3061 aaggtgtatt gtcccaaaac gatgtgctcg ttgctgaaaa agtccgtcag gttctgagtt
     3121 tggcgggctg gagcgatgga caagacgaag gcttttttga cgtccagcga cttccccccg
     3181 aactcgacct ttgtcaagta caactctacg gaaaccatct cgtggttctc tagagacaga
     3241 ttcttgtagt gcaggaacac ggataggttg cgtttcgctt tacccggtaa gggaaagaat
     3301 atgttcaaca cgaaacaact cgttaagaaa actggtagta gggcgcctaa gattccgaag
     3361 aaaagcgcgc gtcgtagttt cctcatcaca ttttccgctc cgtggtcact atttgatgaa
     3421 acgatcgagc ttgtccaccc tgagttgatg gcggcctccg tcaaattcgc tgtggatgaa
     3481 agactccacg caccaaatgg ccaattcttc tcccatcacc cggccgccga gcgcgatgac
     3541 gttggcgttg ttgtgcgcgc gcgccagttt cgccatatcg gggtagcacg ccaatgccgc
     3601 gcgtattccg gggaatttgt tcgccgcgat cgacataccg atgccggttc cgcaaaccaa
     3661 aatcc
//
LOCUS       DAOPXN010000215         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_26708_length_3559_cov_5.674372, whole genome shotgun sequence.
ACCESSION   DAOPXN010000215 DAOPXN010000000
VERSION     DAOPXN010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26708_length_3559_cov_5.674372"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PKM49_09335"
     CDS             complement(<1..200)
                     /locus_tag="PKM49_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M14"
                     /protein_id="HNY83034.1"
                     /translation="MLFKELIRDIPDYKRFFTIDEMDERSKALAAKYPDRVKITCAGY
                     SRKGAPIHVLEIGKGKRNALLFG"
     gene            complement(309..2396)
                     /locus_tag="PKM49_09340"
     CDS             complement(309..2396)
                     /locus_tag="PKM49_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HNY83035.1"
                     /translation="MRKVSLFLLLAVLVLTLSFGQVALEEARPAILKAGILKIVDGSD
                     LTANEFKDAVQKAFPGKEGYVAAGTNAVSRTEFITTLVKVLGLSEEAARYAEVVTMAH
                     DERQVPDYAVGAFTTAYRSNHQLLNYRYGHLLEPSAAITKEEAALSFYMALYPPKVGG
                     TITTAVGADAPGFNTLFTSSGLTWTICNIIADGYIGSNQDGFYTPRMIKRIPSLENGL
                     LVLNDDDSMSVTFELRKGMKWHDGAPVTARDAKFQWEVMTSGAPVTSNSYEMSVDRVD
                     IIDDYTFTIHMKEKSGSGYLGSSVYAYYFGWFQIPEHVYRKDFEEAKKANRWEDFVQK
                     VTRNPIMTGPFKFKEYKEGQYIIMDAFDDYYMGRPNIDTIVMKIIPDADVTYASVKNG
                     ELDFGRYTLTMKQSLQLEKEHSDIFTVYYVQNIAPDLIFTNFRDPDNLSKTNFYFGDI
                     RVRQALLHAINRDAINSLVYSNKGQVCDTWLTPLHIMRDALTDPSVKKYPYNVQKAKD
                     LLAAAGWKAGKGGTLEKDGKPFKFPMIVAAGSTDALTMAQMIQGMLKQVGIELEIDTK
                     PAVLVWDILPQGKFHAVLSGWGYGLSDEAAYYWTEDMIPSEENAFGGTNYTGWANKKS
                     DEYVYKAFAELDFNKKVEYYIKHLAEWSNDLPYIPLVAPPTPLFAKNYIKSFNAGYDN
                     GLGWIIQNWYVDR"
     gene            2617..2964
                     /locus_tag="PKM49_09345"
     CDS             2617..2964
                     /locus_tag="PKM49_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNY83036.1"
                     /translation="MPIIKRQRCGRPKKCRRISLPREDRIFEPLGIAPREMKAVTLSL
                     DELEAMRWVDQLKTSHEAGAKAMGISRQTFGNIIGSARTKIADALINRKAIRIEGGSI
                     HKEEDDESSHSGI"
     gene            2936..3310
                     /locus_tag="PKM49_09350"
     CDS             2936..3310
                     /locus_tag="PKM49_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNY83037.1"
                     /translation="MMKVAIPVSEKNGMKSKTHAHFGSAPVYAIVETEGGEIEWYDNE
                     EAEHTHGSCQPAGKLAQFHIDAVVCQGMGARAIDKLNGLGMKVYVNRRAATVEEVIEE
                     IKQNRLTELSKEGACQQHHGAH"
BASE COUNT          878 a          874 c          926 g          881 t
ORIGIN      
        1 ccgaagagca gggcgttacg cttccccttc ccaatttcga gaacgtgtat cggcgcgcct
       61 ttccgcgaat agccggcaca agtgattttg acgcgatcgg gatacttcgc cgcaagcgct
      121 ttcgaacgct cgtccatctc atcaatcgta aaaaaccgtt tgtaatcagg gatatctcgg
      181 attagttctt tgaatagcat ctgtatactc tcctctttga gatcgttttc gattcaaatg
      241 aagagcgctg aaccgaagtc ccgcgctctt ctgttaacgt tggttatgaa agcctcaagc
      301 gctaacggtt atcgatcgac gtaccagttt tggatgatcc agcccagacc gttatcgtaa
      361 cctgcattga aggatttgat gtagtttttc gcgaataacg gggttggagg agcaaccaac
      421 ggaatatagg gcagatcatt cgaccattcc gccaagtgtt tgatatagta ctcgactttc
      481 ttgttgaaat cgagttccgc gaaggctttg tagacatatt cgtcactttt tttgttcgcc
      541 cagcccgtgt aattggtgcc accgaaggcg ttctcctcgg acggaatcat atcctcagtc
      601 caatagtaag ccgcttcatc cgacaaccca tatccccaac cggagagaac cgcgtggaac
      661 ttgccttgag gtaaaatatc ccagaccaac acggctggtt tggtatcgat ttccaactcg
      721 atacccactt gtttgagcat cccctggatc atctgagcca tcgtcaatgc gtcggtgctg
      781 ccggccgcaa cgatcatagg gaatttgaac ggtttaccgt ctttttcgag ggttccgcct
      841 tttccggctt tccaaccggc tgccgccaat aggtctttgg ctttctgcac attgtagggg
      901 tattttttga cgctcgggtc agtgagcgcg tccctcatga tgtgtaaagg agtaagccag
      961 gtgtcacaca cttggccttt gttgctgtag accaagctat tgatggcgtc gcggttgatc
     1021 gcatgcaaca gggcctgccg aacgcggata tctccgaaat agaaatttgt cttcgacagg
     1081 ttgtcgggat cccggaaatt ggtgaagatc aggtccggag cgatgttttg gacgtagtag
     1141 acagtgaaga tatccgagtg ttctttctct aattgcagcg actgcttcat cgtgagcgtg
     1201 taacgcccga agtccagttc cccgtttttc acggacgcgt aggtgacgtc agcgtcggga
     1261 atgatcttca tcacgatggt gtcgatgttc ggccgcccca tatagtaatc gtcgaaggca
     1321 tccataataa tgtattgccc ttctttatat tccttaaact tgaaaggacc agtcataatc
     1381 ggattgcgag tcaccttctg cacgaaatct tcccaacgat tggccttttt tgcctcttcg
     1441 aagtcctttc ggtagacgtg ttcggggatt tggaaccatc cgaaatagta ggcgtaaacg
     1501 gaggagccga gataaccgct tccggacttc tctttcatat ggatcgtgaa ggtgtaatcg
     1561 tcgatgatat cgactcggtc gacagacatt tcgtaggagt tggaggtcac cggggcgccg
     1621 gaggtcatga cttcccactg gaatttggcg tcgcgagcgg taacgggagc gccatcatgc
     1681 cacttcatgc cttttctcaa ctcaaaggtg accgacatcg aatcgtcatc gttgagcacc
     1741 aacaatccgt tttccaagga gggaatccgt tttatcatac gcggcgtata gaacccgtcc
     1801 tggttcgaac cgatatagcc gtccgcgatg atattgcaga tagtccaggt taacccggaa
     1861 gaggtgaaga gggtgttaaa tccgggggcg tctgcgccca ccgcagttgt gatggtacca
     1921 ccgaccttcg gcgggtagag cgccatgtaa aaggacaagg ccgcttcttc tttcgtgatg
     1981 gcggctgatg gctctaaaag atgtccatac cgatagttca gcagttggtg gttgcttctg
     2041 taggcggtcg taaacgcgcc aaccgcgtag tccggcactt ggcgttcgtc gtgcgccatc
     2101 gtgacgacct ccgcatagcg ggcagcttcc tctgataatc cgaggacttt gaccaaggtc
     2161 gtgatgaact cggtacgaga tacggcgttc gtgcccgccg cgacgtagcc ttcttttccc
     2221 gggaatgctt tttgcaccgc gtctttaaac tcattggcgg ttaagtcaga gccgtccacg
     2281 attttgagga taccggcttt aagaattgcc ggacgggctt cttcgagagc gacttgaccg
     2341 aaagaaagcg tgaggaccaa tactgcaagt aacaagaaca atgacacttt tctcatgttc
     2401 cacccccaaa tagtgttgaa tactatcgta agagtcccaa aatatggact tctacaaacg
     2461 ctttcgggta cggactcgta tattgctgca ttataacata cttcattttg aaaaggaaca
     2521 cccctccgtt ttgcggtgtt acgtttgcaa atcgggtata tatcgaatcg ataaaccacg
     2581 cttgacagga tcgtctccct gttctataat tggcgcatgc caataattaa acgccaaaga
     2641 tgtggaagac cgaagaagtg tcgcaggata agcctaccca gagaggacag gatattcgaa
     2701 cctctcggaa tcgccccgcg agaaatgaaa gcggtgacgc tctctctaga cgaattggag
     2761 gcgatgcggt gggtcgacca gttgaaaacc tctcacgaag cgggagccaa agcgatgggc
     2821 atatcacggc agaccttcgg caatatcata ggttcggcca gaacgaagat agcggacgcc
     2881 ctcataaatc ggaaagcgat ccggatcgag ggcggatcga ttcataagga ggaagatgat
     2941 gaaagtagcc attccggtat ctgagaaaaa cgggatgaaa tccaagacgc atgcccattt
     3001 tggcagcgcg cctgtgtacg cgatcgtcga aacggaaggc ggggaaatcg aatggtatga
     3061 caacgaagaa gccgagcaca cacacgggag ttgtcagccg gcgggaaaac tggcccagtt
     3121 ccacatcgac gcggtggtgt gccaaggcat gggcgctcgc gcgatcgaca aactcaacgg
     3181 gctggggatg aaagtgtacg tgaacagaag ggccgccacc gtggaagagg tgatcgaaga
     3241 gattaagcag aaccggttga ccgaactctc taaagagggg gcctgtcaac agcatcacgg
     3301 ggcccattga ccattaaaaa aaaccggacg gatgtccggt tttttttaat gccctcgatg
     3361 ctcgaaagca tactcaccca ttgtggctta gaaggctttg tcatttatca taggtcaccc
     3421 acccgtatcc tacgaacagg cgagtttttt ctcctagagc cactccggtt atttgtgtgg
     3481 gccaagttat gagatgtaat caagaaacag gctcttcgac aatttacaag acgtacttaa
     3541 taaacaactt ttatcgtaa
//
LOCUS       DAOPXN010000216         3533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_26990_length_3533_cov_4.748706, whole genome shotgun sequence.
ACCESSION   DAOPXN010000216 DAOPXN010000000
VERSION     DAOPXN010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26990_length_3533_cov_4.748706"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..626
                     /locus_tag="PKM49_09355"
     CDS             207..626
                     /locus_tag="PKM49_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83038.1"
                     /translation="MANQKHLWAPMALVLAVLGLVGFLFIDTAREKEIVFEADRLVIP
                     GVFGAIVRRDQVESVTLVERIPKILLRTNGASVGHIRFGLFKLEGTEKAHLTVMDKDN
                     PPFVLIASNNRALYINFAQKEKTLQLFEEIRAWARGK"
     gene            636..1514
                     /locus_tag="PKM49_09360"
     CDS             636..1514
                     /locus_tag="PKM49_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNY83039.1"
                     /translation="MSESTGFFSKKNIREILFSSLGVLLTAIGLVSFLIPFHIAAGGV
                     SGIAIILSHFLPLPVGVLMYLLNGVLFLISFFVIGREFGLRSVYCTFLLNFLIDLFDR
                     GIPFPKYTGDDLILSALLGVIISALGMAITFNQNSSTGGTDIVARILNKYAGTSMGLS
                     LLLIDLVIGVFAGVAYETRVGMYSVLSVILNGLTIDYIIRVLNNTLTVLIISEKNNAI
                     SDFIMKTLERGVTRLKGEGAWTQNEKNMLYIALKRKEMTDTIRQIREIDPKAFIVVQE
                     AAHVIGEGFTHISRIF"
     gene            complement(1576..2796)
                     /gene="thrC"
                     /locus_tag="PKM49_09365"
     CDS             complement(1576..2796)
                     /gene="thrC"
                     /locus_tag="PKM49_09365"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNY83040.1"
                     /translation="MNHPMTYLLRCVNCGKEYVPDRELYTCPDCLDRMGTLEVVYSLT
                     SKTQWYRSEYPGIWAYADLLPIVKDPLPPSLYVGNTPLVRAPRIEREFGIQELWIKDD
                     GKNPTASFKDRASVVAIAKALEYGYTDLFAASTGNAASSLAGLCAPAGLKAHIYLPET
                     APEAKIAQLLIYGAQVFAVRGSYDEAFDLSLREGFQKGWYCRNSAINPYLLEGKKTGT
                     FELIRELNMRAPDIILVGVGDGTVYSSIHKAAEELAQLRLIGRIPTIIGIQSEGANAV
                     CRTFYRGEPFTPADIQDATSLADSIAVGKPRDVIKACQWAERHNGFFLTVSDEEIGKS
                     IVELARASGVFAEPAGAAAYAGLKRLKDEGFLNAKARVALMITGNGLKDARAAVPYLA
                     ERVIPVSVEGIREK"
     gene            complement(2793..>3533)
                     /locus_tag="PKM49_09370"
     CDS             complement(2793..>3533)
                     /locus_tag="PKM49_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNY83041.1"
                     /translation="ADAFELSTHYLGRTLDPIAKTIRAAARATDRPIFIKLSPHIPDP
                     VEFAKMVKAEGGYGLVAINSLGPVYPVRANLSTSPLGSADGFGWISGPVIKPLSLAII
                     RKIATSVDIPIIGVGGVGSAQDVIDFLKCGASAVQMLSAAMLKGKTLYRKIISALPAV
                     LESLGFSDMRSVQGLALKSNTATLFERRLPVIDPKLCTLCNRCVENCPYDAMENRDRR
                     IIVDPALCFGCGLCESRCPARAISGVLL"
BASE COUNT          776 a          971 c          960 g          826 t
ORIGIN      
        1 atgctctcgt gggttatcgc ggggtaagtg gtttttacga ccccgcgatc cgccctttgc
       61 ccttctcaaa agcgtatcgc gccgttgcga aaagaatggg cgcttacgcc gcgatgacaa
      121 aatggacaaa aaatcacgcg atcgattctg gccttgtttc ttgactacac cttataactt
      181 gacacacacg aattcggagg ttggatttgg cgaatcaaaa acacttatgg gcgccgatgg
      241 ccttggtgtt agccgtttta gggctggtcg gttttctctt tatcgataca gcgagggaga
      301 aagaaatcgt ttttgaagcc gaccggcttg tgatccccgg agtcttcggc gcaatagtac
      361 ggcgagacca agtcgaatcc gtgacgctgg tcgagcgaat acccaaaatc ctcttaagaa
      421 caaacggagc gagcgtgggc cacatccgtt tcgggctttt caagttggaa ggcacggaaa
      481 aggcgcatct caccgtaatg gacaaagaca acccgccctt cgtcttgatc gcctccaata
      541 accgagcgct ctacatcaac ttcgcgcaga aagaaaaaac gttgcagctc tttgaggaga
      601 ttcgcgcttg ggcgcgagga aagtgaggaa atacaatgag cgagagtacc ggcttcttct
      661 ctaaaaaaaa tatacgcgag atcctctttt cctctttggg cgtactatta actgccatcg
      721 gactggtttc cttcctcatt cctttccata tcgcggccgg cggggtgagc ggtatcgcga
      781 tcattctctc gcacttcctt ccgcttcccg ttggcgtgct gatgtacctg ttgaacggag
      841 ttctgttctt gatttctttc ttcgtgattg ggagagagtt tggtctgcgc agcgtctatt
      901 gcactttcct gctgaacttc ctcatcgatc tgtttgaccg gggaatcccg ttcccgaagt
      961 atacaggtga tgacttgatc ctgtccgcgc ttttaggggt catcatctct gctctgggaa
     1021 tggcgatcac cttcaaccag aactcctcca cgggcggcac ggacatcgtt gcccgcatat
     1081 tgaacaagta cgcggggacc tcaatggggt tgtccttgct cttgatcgac ctggtgatcg
     1141 gggtcttcgc cggggtggcg tatgaaacac gtgtcggtat gtattcggtc ttatcggtta
     1201 tcctaaacgg gttaacgatc gactacatca tacgcgttct caacaacacc ctgaccgtat
     1261 taatcatctc ggaaaagaac aacgcgatca gcgatttcat tatgaaaacg cttgaacgag
     1321 gcgtgacccg cctaaaaggg gagggtgcgt ggactcaaaa cgagaagaat atgctttaca
     1381 tcgccctaaa acgaaaagag atgaccgata ccatacggca aataagagaa atagacccga
     1441 aggcctttat cgtcgttcaa gaggcggcgc acgtgatagg agaagggttt acccacatct
     1501 ccaggatatt ttagcgccct ccaaacgcgc tgcccggcgt atcttatggc gagtctttta
     1561 cgaagcgggg gccggtcact tctctcttat accctcgacg gagaccggta tcacgcgttc
     1621 tgcgaggtag gggaccgcgg cacgagcgtc tttcaacccg ttccctgtga tcatcagcgc
     1681 gacacgcgcc ttcgcattca gaaatccttc gtcttttaat cgcttcaaac cggcgtaggc
     1741 ggcggcgccg gcgggttccg cgaagactcc ggaggcgcgc gccagctcga caatcgattt
     1801 tccgatctct tcatcgctga cggtcaagaa aaaaccgtta tgcctttccg cccactgaca
     1861 cgccttgatc acgtccctcg gcttaccgac tgctatcgag tccgccagac tcgtcgcgtc
     1921 ttgtatatcc gcaggcgtaa aaggctcacc tcgataaaac gttcggcaaa cggcgtttgc
     1981 cccttctgac tgtatcccaa tgatggtcgg gattcggccg atgaggcgca gttgagccaa
     2041 ttcttcggcc gctttgtgta tgctgctgta aacggtgcca tcgcccacgc cgaccagaat
     2101 gatgtcaggc gcgcgcatat tcagctctcg gatcaattcg aaggttcccg ttttcttccc
     2161 ttccagcaga tacggattta tcgcggaatt gcgacagtac cagcctttct gaaacccttc
     2221 tcgcaaggag aggtcgaagg cttcgtcgta actcccacgg acggcgaaga cctgagcgcc
     2281 gtagatgagt aattgcgcga tcttcgcctc cggtgcggtt tcgggtagat agatatgggc
     2341 tttcaatccc gcaggcgcgc acagtcccgc gagcgaactc gccgcgttcc ccgtggaagc
     2401 ggcgaagagg tcggtgtatc catactcgag ggcttttgcg atagcaacca cgctagcccg
     2461 gtccttgaaa gaggccgtcg gatttttccc gtcgtctttg atccacagtt cctgtatgcc
     2521 aaactctcgc tcaattcgtg gagcgcgtac caggggggtg ttccccacgt acagcgaggg
     2581 cggcagcgga tccttcacga tgggcaagag gtctgcataa gcccatattc ccgggtattc
     2641 ggaccgatac cattgagttt ttgaggtgag cgagtagacg acttctaatg tccccatacg
     2701 gtctaaacag tccggacacg tgtacagctc gcggtcgggg acgtactcct tcccacaatt
     2761 tacacagcgc aacaagtaag tcatcggatg gttcataata aaaccccgct gatcgcccgc
     2821 gcggggcaac ggctttcgca aaggccgcat ccaaaacaca gggcgggatc gacgatgatc
     2881 ctcctgtcac ggttttccat cgcgtcataa ggacaattct ctacgcatcg attgcataag
     2941 gtgcacagct tgggatcgat caccggtagg cgcctctcga aaagggtggc ggtgttactt
     3001 ttcaacgcta acccttgtac gctccgcata tccgaaaacc cgaggctctc cagcacggcc
     3061 ggtagcgcgg agataatttt ccgatacaag gtctttcctt tgagcatggc ggcggagagc
     3121 atctggaccg cgctggctcc gcacttgagg aagtcgatca cgtcctgagc ggaaccgacg
     3181 cctccgacgc cgatgatggg tatatcgacc gacgtggcga tcttccgtat gatcgcgagc
     3241 gacagaggtt tgatgacagg gccggagatc caaccgaatc cgtccgcgct acccaaagga
     3301 gaagtggaga ggttcgcccg gaccggatag accggcccta aggagttgat ggcgacgagt
     3361 ccgtatcccc cttccgcttt gaccattttg gcgaactcga ccgggtccgg tatatgggga
     3421 ctgagtttga tgaaaatggg acggtccgtc gcccgcgcgg cggcgcgtat cgttttcgcg
     3481 ataggatcga gggttcggcc caggtagtgc gtgctcagtt cgaaggcgtc cgc
//
LOCUS       DAOPXN010000217         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_27117_length_3521_cov_5.011252, whole genome shotgun sequence.
ACCESSION   DAOPXN010000217 DAOPXN010000000
VERSION     DAOPXN010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27117_length_3521_cov_5.011252"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PKM49_09375"
     CDS             <1..246
                     /locus_tag="PKM49_09375"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNY83042.1"
                     /translation="DEVLGIDNFDFLIISAHRKESKAYLSSYKGKLNEILDGIERQAI
                     LQYLYKNEGNRERTAKELGISVRSLYYKIKRYRIDRL"
     gene            303..1082
                     /gene="folE2"
                     /locus_tag="PKM49_09380"
     CDS             303..1082
                     /gene="folE2"
                     /locus_tag="PKM49_09380"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNY83043.1"
                     /translation="MLDVQSEQDHRNLPLDRVGIKKMKYPIALRGKEKGTQHTIAEVT
                     MSVDLPLHFRGTHMSRFVEVLHSHAHNMELKNLKTILSDMKQKLSASRAHLDLEFPYT
                     IEKMAPVTGILSYMVIPCQFNATLDETDRFELVYGAKVPIQTVCPCSKAISSRGAHNQ
                     RANVTILLISKKIIWIEDIVKIAEQGASSPVFPLLKREDEKFVTENGYDHAKFVEDVA
                     RDIGVVLRESESVSWYSVEVSSMESIHDHDAFAYLEGGQRT"
     gene            1079..2689
                     /locus_tag="PKM49_09385"
     CDS             1079..2689
                     /locus_tag="PKM49_09385"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83044.1"
                     /translation="MILERALLHDIDFLDRATVDFIQPSALLSSVLPSGALGQAAALR
                     LRPFVRGQEQALEAHFVKLAFLENAYEKTFGTLRESLKQFRNITTSLSKLRSASPVNL
                     ADLFEIKRFLLLYERLREDFGIESLPGFRFIPLTSALRVLDPEEKRQMFFSLESDYLR
                     DLRSELEKASKQEAIELKQHCDLLQKQYEIPVRNQREFVLSRTDIRNLALQKCEWISL
                     IRESTFSIHYQIIGGERTRTLQMKKQEIHAEIEVEEQRLIEEIHLNLQKHAAEMMAQM
                     TEVGALDLALALVAFKKAYHCVYPTVLSGSNTSSAPLTLVLENGRDPILEAERRQKGL
                     PYDPLTISLKEGATLVVGANMGGKTTALRTLGLLVLLAHMGIPTPTDRFELPLFSRIY
                     ALYKTKEQDGLSGFGSEIVRAKSAFSPFSLSLIDEFASSTNPSEGEALAAALVEHAHG
                     VPNQISLFVTHYSKPLSITAEVYTTGILRSIDGQNYENLEALYRLIDHRLHRLAVREI
                     PRGAFTVAKALGFSDTILEKATDYLNKS"
     gene            2707..3507
                     /locus_tag="PKM49_09390"
     CDS             2707..3507
                     /locus_tag="PKM49_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNY83045.1"
                     /translation="MSTEKEEGYVKHKLLVLSGKGGVGKTTVSVNLAAALALQGYRVG
                     LMDVDIHGPNVAKMLGLEEKVHIASEDGKIIPHTLFQSLKVISLGSFVPKGKPIIWRG
                     PLKNKTIEQFVDDVEWGDLDVLVVDTPPGTGDEHLGIMQALGSIDGAILVTTPQTVSK
                     DDAKRAGEFVKVMESRILGIVENMSYFVCPTCKTVHYLFGKDGGQILADELETVVLTK
                     IPQDPMMIVSGDAGKPVPFFFRETAMEKHFYELSEMILALLGEGSREG"
BASE COUNT          919 a          898 c          934 g          770 t
ORIGIN      
        1 gatgaggtgc tagggatcga caatttcgac tttttaatca tctccgctca ccggaaagaa
       61 tcaaaggcgt atctatcctc ttacaaagga aaactgaacg agatcttgga tggaatagaa
      121 cggcaagcga tcctgcaata cctgtacaag aacgaaggca atcgcgaacg tacggcaaag
      181 gagttgggca tcagtgtgcg ctctctctac tataaaatca agcgttatcg cattgaccgt
      241 ctctgaattt gacgatacgg gcggcgggac gagaactaaa agccttgttt tgaggtgatg
      301 ttttgctaga cgtacagagt gagcaggatc atcgtaacct gcctttggat agggtcggca
      361 tcaaaaagat gaaatacccg atcgcgttgc gtgggaagga gaagggaacc caacacacca
      421 tcgcggaggt cacgatgtcc gttgacctgc cccttcactt ccgcgggacc catatgagca
      481 gattcgtgga ggttcttcac agccacgccc acaatatgga gttgaaaaac ctcaagacga
      541 tcctttcgga tatgaaacag aagttgtccg cctcaagagc gcatttggac ctcgaattcc
      601 cttacacgat agagaaaatg gcccccgtca cgggaatcct cagctacatg gtgatcccct
      661 gtcaattcaa cgcgaccctg gacgaaaccg atcgcttcga gctcgtttac ggagcgaagg
      721 tgccgataca gacggtttgt ccgtgttcca aagccatcag ttcccgaggc gcgcataacc
      781 agcgcgcgaa cgtgacgatc ctactcatca gcaaaaagat catttggata gaggatatcg
      841 tcaaaatagc cgaacaaggg gcttcatcgc cggtctttcc gctgttgaaa cgggaggacg
      901 aaaagttcgt cacagaaaac ggctacgatc acgccaaatt cgtggaggac gtggccaggg
      961 acatcggggt cgtgttaaga gaatctgaga gcgtttcttg gtacagcgtc gaggtgagca
     1021 gtatggagtc cattcacgac cacgacgcct ttgcctatct ggagggcgga cagagaacat
     1081 gatactcgaa cgcgcgcttc tccacgatat agatttcctg gatcgagcca ccgtcgactt
     1141 catccaaccc agcgcgttgc tgtcctccgt cctcccatcc ggcgccttgg gccaagcggc
     1201 cgcgcttcgc ttgcgaccct tcgtgagggg tcaagagcag gcgttggaag ctcatttcgt
     1261 caaactggct tttttggaaa acgcttacga aaagaccttt gggacgctgc gagaatcttt
     1321 gaagcaattt agaaacatca cgacgagcct ttcgaagctc cgatcggcca gcccggtcaa
     1381 cctggccgac cttttcgaga tcaaacgatt tttactgctt tacgagcggc ttcgagaaga
     1441 tttcggaata gaatccttac caggcttccg cttcatcccc ttgacgtcgg ccttacgtgt
     1501 tttagacccc gaggaaaaac gccagatgtt tttctctttg gaatccgact accttcgcga
     1561 cctcaggagc gagttggaga aggcgtccaa acaagaagct atagaattaa aacagcattg
     1621 cgacctgctt caaaagcagt acgaaatccc cgttcgcaac cagagggagt tcgtcctctc
     1681 cagaacagat atacgaaacc tcgcgttaca aaaatgcgaa tggatctcac tgattcgaga
     1741 aagcaccttt tcgattcatt accaaatcat cggtggcgag aggacacgaa ccctccaaat
     1801 gaaaaagcag gaaatacacg ccgaaatcga agtcgaggaa caacgcctca tagaagaaat
     1861 acacctaaac ctccaaaaac acgcggccga gatgatggct cagatgacgg aggtcggcgc
     1921 cctagacctc gccttagcgc tcgtggcctt caaaaaggcc tatcattgcg tttacccaac
     1981 ggtcttgagc gggtctaata cctcttcagc gcccctaact ttggtcttgg aaaatggaag
     2041 agacccaatt ttggaagcag aacgccggca gaaaggcctg ccttacgatc ctttgacgat
     2101 ttccttaaaa gagggggcga cattggtcgt cggcgccaat atggggggga aaacgacggc
     2161 gttgagaacc ctcgggttac tggttttgtt ggcccacatg gggatcccca cgccgacaga
     2221 ccgtttcgag ctgcccctgt tctcccgtat atacgcgctc tataaaacca aagagcaaga
     2281 cggattgagc ggcttcggtt ccgagatcgt acgggcgaag tcggccttct ctcctttctc
     2341 gctttccttg atcgacgagt ttgcgtcatc tacgaatccg agtgagggag aggcgctcgc
     2401 cgccgcttta gtggagcacg cccacggcgt gccgaaccaa atctcgctct tcgtcacgca
     2461 ctattcgaag cctttatcga ttacggccga ggtctacacg accggcatcc ttcgttccat
     2521 cgatgggcaa aactacgaaa acctagaggc cttataccgt ttgatcgacc acaggctcca
     2581 ccgtctggcg gtccgcgaaa tccccagagg ggcgttcacg gttgccaaag cgttggggtt
     2641 ttctgataca atactggaaa aagcgaccga ctatttaaac aaaagttgag ggaaaggaga
     2701 tctcgcgtgt ctacggaaaa agaggaaggt tatgtaaagc acaaactgct ggtgttgagc
     2761 ggaaaaggag gggtcgggaa aacgaccgtt tccgtgaacc tcgccgccgc cttagccctt
     2821 cagggttacc gggttggctt gatggatgtg gatatccatg ggccgaacgt ggcgaagatg
     2881 ttagggttag aagagaaagt ccatatcgcc tccgaagacg ggaagatcat cccgcacacc
     2941 ctttttcaaa gtttgaaggt catttctttg ggcagcttcg taccgaaagg caagccgatc
     3001 atctggcggg ggccattaaa aaacaagacg atagagcagt tcgtcgatga tgtggaatgg
     3061 ggggacttag atgttttggt cgtagacacc cctcccggca cgggcgatga gcacctcggc
     3121 attatgcaag ccttgggcag catagacggg gccattttgg tgacgacacc ccaaaccgtc
     3181 tctaaagatg acgccaagcg agcgggagag ttcgtgaaag tgatggaatc gcggattttg
     3241 ggcatcgtcg aaaacatgag ctatttcgtt tgcccgacct gtaaaaccgt tcattaccta
     3301 ttcggtaaag acggagggca aatcttggcc gatgaattgg agaccgtcgt tttaaccaaa
     3361 atcccgcaag acccgatgat gatcgtttct ggggacgcgg gaaaaccggt gccgttcttt
     3421 tttcgggaga ccgccatgga aaagcacttc tacgagctaa gcgaaatgat tctcgccctt
     3481 ttgggagaag gaagtcgtga aggttaaccg gataccacga g
//
LOCUS       DAOPXN010000218         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_28135_length_3426_cov_5.104420, whole genome shotgun sequence.
ACCESSION   DAOPXN010000218 DAOPXN010000000
VERSION     DAOPXN010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28135_length_3426_cov_5.104420"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PKM49_09395"
     CDS             <1..464
                     /locus_tag="PKM49_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY83046.1"
                     /translation="GRGFAVVADEIRKLAEESKRATSNIGSILKEIQKDAGESNQATE
                     KTTVLVQAVNDKARAVAQQFKQLKDMMEKTTAMIENLTATSEEQGAAAEEMASAMNTS
                     TKAVHEISEQIRQMTQGIEQQAQGAQQISASAQELDSLAESLDTQVKRFKV"
     gene            1083..2249
                     /locus_tag="PKM49_09400"
     CDS             1083..2249
                     /locus_tag="PKM49_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY83047.1"
                     /translation="MKKLFLFFGINRSIFGMLVLVIFVGLGEKMAERFLPLYLLALGG
                     SVFAVGALNALDNLLSALYSFPGGYLSDKLGYKRALIVFTAIAMGGYLIVILIPTWVA
                     VFIGSFFFISWTAISLPAVMSLVSNVVPKEKRTMGVSVHSLIRRIPMALGPIIGGIFI
                     GAMGKVTGVRVAFACAFGLAFIAILAFWKLVDDQPATKPIKIKEAWRGISPSLRTLLI
                     SDILIRFAEQIPYAFVVVWVVEVQKLSALQFGILTTVEMLTAMTVYIPIAYLADRTRK
                     KPFVLTTFVFFTAFPLALMFAKSFFALILVFILRGLKEFGEPTRKSLIMDLAPEDRKA
                     ATFGTYYLIRDVVVSGAALTSAYWWSMAPEVNFLVAFGCGIAGSLFFLFKGKDS"
     gene            2999..>3426
                     /locus_tag="PKM49_09405"
     CDS             2999..>3426
                     /locus_tag="PKM49_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNY83048.1"
                     /translation="MPEIWDVYTKNREITSRKHVRGSPLPEGDFHLVVEIWIFRTSGQ
                     ILVMKRHPDKPWPNMWECTGGSAVEGEDTAAAAVREVEEEIGVHLVASEGRIVHQYTF
                     EDSHYDVWCFRKDIPLEDLVFQEGEVVDAKYVTPNELRVLL"
BASE COUNT          839 a          837 c          942 g          808 t
ORIGIN      
        1 cgggtcgggg cttcgcggtg gtggcggacg agatacggaa gttagccgaa gagagtaaaa
       61 gggcgacttc gaatatcgga tcgattctaa aagaaatcca aaaagacgcc ggagaatcca
      121 accaagcgac ggaaaagaca accgtcttgg tccaagcggt caacgataag gcgcgagcgg
      181 tggcgcagca gttcaaacaa cttaaggata tgatggaaaa gacgacggcg atgatcgaga
      241 acttgacggc gacctctgaa gaacagggag ccgcagcaga agagatggcc tcagcgatga
      301 acacctcgac gaaggccgtt cacgaaatct ccgagcagat ccgacagatg acgcagggca
      361 tcgaacaaca ggcgcaaggc gcacaacaga tcagcgcgtc ggcacaagaa cttgattcat
      421 tggccgagag cctcgacacg caggtcaaac ggtttaaagt atagacaaag cgggagctct
      481 gagaacgcct aggaacgccg aacttctctg ttggcaggtt taaaaagata gtctcgcacg
      541 gaggacacag agggcacgga ggaaagaggg gaaaggggat tatctagacc gttaaaaggg
      601 aaatacgctg aaagctgttc tcggaggtta tcgcggggta agtgattttt acgaccccgc
      661 gatctgcttt atgtcattct taaaagggtt tcgcgcagtc gcgagaagaa agggcgctta
      721 cgccgcgatc cccgtgggag ttatcgcggg gtaaccagcg ggctgacttc ggaagcccac
      781 gaagtctcgc gatacggact gccgcatttc tattttcaca aaccgtgcca aactaaagtc
      841 tggcattgcc aggtgtatcg cgccgtcgcg agaagaaggg gcgcttatac tgcgatgctg
      901 cgtatggaaa cgccctgagt gaaaaaaaaa ccctgccgaa ctaaagttcg gcgttcccag
      961 ggtactctct gaaagcgaaa gccgtgctga gcgaaaaatt cgacgttcgc agggtcttgt
     1021 caataaaagt aaactaagtc gcacgataaa caaaccgtga tctttcccga ggtggggtat
     1081 agttgaagaa gcttttccta ttctttggta tcaatcgtag tatcttcggt atgcttgtct
     1141 tagtcatctt cgtaggtttg ggggagaaga tggccgagcg gtttttaccc ctttacctat
     1201 tggccctcgg cgggagcgtc tttgcggtag gggcgttgaa cgccttggat aacctgttga
     1261 gcgcgttata ctcttttcct ggagggtacc tgagcgataa gctggggtat aaacgcgctc
     1321 tcatcgtgtt caccgcgatc gctatgggag gatacctcat cgtgatcttg atccctacgt
     1381 gggttgcggt tttcatcggg tcgttctttt ttatctcatg gaccgcgatc tcgctaccgg
     1441 ccgtgatgag cttggtgtcc aatgtcgtgc cgaaagagaa acgaacgatg ggcgtttccg
     1501 tacactccct catccgtagg attccgatgg ctttgggccc aatcataggc ggcatcttca
     1561 tcggagcgat gggcaaggtc acgggggtgc gcgttgcgtt cgcttgcgcg ttcggtttgg
     1621 cgttcatcgc tatcctggcg ttttggaaac tggtggacga ccagccggct acgaaaccga
     1681 tcaaaatcaa agaggcctgg cgcggaatct ccccttcgct gaggactctt ctgatttcgg
     1741 atatcctgat ccggttcgcc gaacagatac cgtacgcgtt cgtggtcgtt tgggttgttg
     1801 aggtccagaa actctccgcg ttacaattcg gcatccttac gaccgtcgaa atgttgaccg
     1861 cgatgacggt gtatatccca atcgcctacc tcgccgaccg aacccgtaaa aagcccttcg
     1921 tcctcacgac tttcgtcttc ttcaccgcat ttccactcgc tttgatgttt gcgaagagct
     1981 tcttcgcctt gatcctcgtc tttatccttc gcggcctgaa agagttcgga gaacccaccc
     2041 gcaagtcctt gatcatggat ttggcgcccg aagacaggaa agccgcgact ttcgggacct
     2101 attacttgat tcgggacgta gtcgtgtccg gggctgccct aaccagcgcc tactggtgga
     2161 gtatggcccc ggaggttaac ttcctggtcg ccttcgggtg tgggatcgct ggaagcctgt
     2221 tttttctatt caaaggtaaa gacagttaat cgccttgtcg catcaccgct ctattcgcgg
     2281 ataagaccga gtttccgtaa aggatgggaa taccgatggc tttcgcgata gtgttgaaaa
     2341 gcgaaggatc gccgtttcta taacgaatac gcgctccgtc gcagcttgtc ggtctaggtt
     2401 ttagtgggat atcggtttaa aaatcgaaca acgccaccgc cgaggagtcc ctgggctttt
     2461 ttcgcagacg aagtttataa caatcccggc attttccaga tgatcgtagc tccggtacgc
     2521 ggggtatcat attttcccgc gcctgaagcg acctttttcc cgtccggtga ccaagaaata
     2581 ctccaaaccg gtaaggagtt gtgttcttcc caagcgtatc gttcccacgt ttttgtatcc
     2641 caaagataaa ttttcttgtc ctcttaagtc cggtaaacga aatagacctc cccgcccttc
     2701 gggcaccctc cgaaggagag gaattgaaca tggtgtcaac accggtaata aaataagaaa
     2761 gcaccggtaa aaaatggggt ttcaccctat caatacgata agatatgcgg aggataacga
     2821 ttggaagggt ttcgcgagag cggtgcctgt ttcttcccta cacagaaaaa ccgtaggcca
     2881 ttgtattcgc ccgggataac acctctctct gtgttacgct aacatataag gatcccctcg
     2941 ccgcacgttc gatgagacgt cgaacggtcc ctcgacttta tgaaaaggag aaaccacgat
     3001 gccagagatt tgggacgttt atacgaaaaa ccgtgagata acttcccgaa agcatgtcag
     3061 aggttcccct ctcccggaag gggattttca tctagtcgtc gagatctgga tcttccggac
     3121 atccgggcag atattggtga tgaaaagaca tccggataaa ccgtggccga atatgtggga
     3181 atgtacggga ggatccgcgg ttgaggggga agacaccgct gctgccgccg tacgggaagt
     3241 cgaagaggag atcggcgttc atttggttgc ttcggaaggg cgaatcgttc accaatacac
     3301 ttttgaggat tctcattacg atgtttggtg tttcagaaaa gatatccccc tggaagacct
     3361 ggtatttcaa gaaggtgaag tcgtggacgc aaaatacgta acaccgaacg aacttcgggt
     3421 tctcct
//
LOCUS       DAOPXN010000219         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_29766_length_3286_cov_3.451563, whole genome shotgun sequence.
ACCESSION   DAOPXN010000219 DAOPXN010000000
VERSION     DAOPXN010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29766_length_3286_cov_3.451563"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKM49_09410"
     CDS             <1..482
                     /locus_tag="PKM49_09410"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HNY83049.1"
                     /translation="FNLYHPLAFYSTFFTIKGWDFDLSVVLKGPEAVRESLLSTNGGK
                     NGETKSRQKAEGERFVHEVALEMLLRGFGFLPVDIIRSHPYRFEIEGQSLRIPLNKVP
                     GLGEKVALSIQQAREAKPFSSIEDVKKRTSVSNTVIDLLKQYNAFGDLSDSAQYALF"
     gene            503..1231
                     /gene="pxpB"
                     /locus_tag="PKM49_09415"
     CDS             503..1231
                     /gene="pxpB"
                     /locus_tag="PKM49_09415"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HNY83050.1"
                     /translation="MRILPCGFDGALIEFSQRISPSIHRRTIRFSKAIEDARILGVLE
                     VVPSYCSLLLRYDPLLTEFAALAHQIRALKRQSGSGGAPEGPVRYIPVRYGGDAGPDL
                     IDSAKACVLSPEQLIATHLSKTYRVYAIGFLPGFPYLGLLPKSLSLPRLDIPRKRVTK
                     GSVAIASRQTGIYPRESPGGWRLIGQTPFELFCETEDPPTLLQTGDRVRFVAIDGAED
                     EAIRRRVSQGNWMRFEREWRQGAL"
     gene            1228..2169
                     /locus_tag="PKM49_09420"
     CDS             1228..2169
                     /locus_tag="PKM49_09420"
                     /inference="COORDINATES: protein motif:HMM:NF014663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83051.1"
                     /translation="MKLLTAGFFLLPVGAPRYGRERFGIGPGGPMDTRALRFNQLLLG
                     NREDDRALEMAGPLPSFCFEEAVSVAITGNPVKIFLDGHPLAMRRSFNVRPGSALTFE
                     RIEAGGWTYLGVAGGIEGIANGEVQLKAPRLNDWSAFLPESLYCERSKDEPIRVVAGP
                     EWDWFDTRARRRFFEQSYRVDSTDRLGCRLKGEPVYTRKPKELISSPVSAGVVQITGG
                     GQPVVLMAGRPTIGGYPRIAFLAPTDLSRLAQTAEGQAVRFREITITEAERTYREETE
                     WMARLERVLENQREPLLYSRSVRVNGHPFYVQITPTD"
     gene            complement(2182..2844)
                     /locus_tag="PKM49_09425"
     CDS             complement(2182..2844)
                     /locus_tag="PKM49_09425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNY83052.1"
                     /translation="MANPTYRREALEEEFGKLERSSPLVELNVEADSIITTVGRKAAD
                     WLDLQKTNTLLDIGTGLGRWAFFYAPLCKEVVGIDISEPLIRQAREAAHQAGVSNVRF
                     YRGSFENLIESEEGADVRPNKVLCVYSFHHLTDPMKAAFLERLAQRWPQPLTLVIADL
                     MWFVDPSRLRESWDDVFYDEGDTDFPSNAFWLQEAARPYFDRTLLVAVHPLVGLLVAQ
                     KG"
BASE COUNT          700 a          963 c          944 g          679 t
ORIGIN      
        1 atttcaatct ctaccaccca ttggccttct attcgacctt tttcacgatc aaaggatggg
       61 acttcgacct ctccgtggtg ttgaaaggcc cagaggcggt gagagagagc ctcctcagca
      121 cgaacggcgg aaagaacggt gagacgaaga gccgtcagaa ggccgaagga gagaggtttg
      181 tccacgaagt ggcactggaa atgcttctga gagggttcgg cttcttgccc gtcgatatca
      241 tccgctcgca tccctaccgt ttcgagatcg aaggccaatc ccttcgaatc cctctgaaca
      301 aggtcccggg cttgggagaa aaggtcgcgc tatccattca gcaagcgagg gaagcgaagc
      361 cgttctcttc gatagaggac gtgaaaaaac gcacctcggt ctccaacacc gtgatcgacc
      421 tcctcaaaca gtacaacgcc tttggcgatc tctcggactc ggcgcaatac gcgctcttct
      481 gagcgcgaag aggaaaaggc ttatgagaat cctcccttgc ggcttcgacg gggcgctgat
      541 cgaattttct cagaggatca gtccatcgat ccatcggcgg acgatcaggt tctcaaaggc
      601 catcgaagac gcgcggatac tcggcgttct cgaggtggta ccctcctact gctcgctcct
      661 cctgcgatac gaccccctgc tcaccgagtt tgctgcgctc gctcaccaga tacgcgcgct
      721 gaaaaggcaa tccggttcgg gcggcgcgcc cgagggaccc gtgcgctata taccggtgcg
      781 ttacggaggc gacgccgggc ccgatctgat cgatagcgcg aaggcctgcg tgctctctcc
      841 cgaacagctg atcgcgacgc acctttcgaa gacctatcgc gtctacgcga tcggcttttt
      901 accgggtttc ccatacctcg gtctgcttcc caaatccctt tcccttccgc gattggacat
      961 tccccgtaag cgggtcacca aagggtccgt ggcgatcgcc tctcgtcaga cgggcatcta
     1021 cccgcgcgaa agcccgggcg gctggcgtct catcggacaa acgcctttcg aattgttctg
     1081 tgagaccgag gacccgccaa ccctgctgca aactggagat cgggtgaggt ttgtcgctat
     1141 cgatggcgcc gaggacgaag ccatccgccg ccgcgtgtca cagggaaact ggatgcgttt
     1201 cgagcgcgag tggagacaag gggcgttatg aagcttctca ccgccggatt tttcttgcta
     1261 cccgtgggcg ccccccgata tggtcgcgag cgattcggga tcgggccggg cggtcctatg
     1321 gacacgcgcg cgctccgttt caaccagctg cttctcggaa atagagagga cgaccgggcg
     1381 ctcgaaatgg cgggtcctct gccttccttt tgctttgagg aagcggtcag tgtagcgatc
     1441 accgggaacc ccgtaaaaat cttcctcgat gggcatccgc tggcgatgcg ccgttctttc
     1501 aacgttcgac caggttcggc gttaaccttc gagcgtatcg aagcgggggg gtggacctat
     1561 ctcggagtcg caggcggaat cgaagggatc gcgaacggcg aggtccagct aaaagcgccg
     1621 aggctcaacg attggagcgc cttccttccg gaatctctct attgcgaacg ctcgaaagac
     1681 gagccaatac gggtcgtcgc gggtccggag tgggattggt ttgacacccg agcgcggcgg
     1741 aggtttttcg agcagagtta tcgagtcgac tcgaccgacc gcttgggttg ccggttgaaa
     1801 ggggagccgg tctatacgcg caaaccgaag gagctgatct cctcgccggt gagcgccggc
     1861 gtcgtgcaga taacgggcgg tggacaaccg gtcgtactga tggctgggag acccacgatc
     1921 ggcggatacc caaggatcgc cttccttgcc cccaccgatc tctctcggtt ggcgcagact
     1981 gcggaagggc aggcggttcg ctttcgggag atcacgatca cagaagcgga acgaacctac
     2041 cgtgaagaaa cggaatggat ggcacgacta gagcgggtcc tcgaaaacca acgcgaaccg
     2101 ctcctctact caagatccgt ccgggtcaat gggcatccgt tttacgtaca aatcacgcct
     2161 accgattaaa cttcgaggcc atcaaccctt ttgcgcaacc agaaggccaa ctaacgggtg
     2221 cactgcgacc aaaagcgtcc gatcgaaata agggcgagcg gcctcttgca gccagaaggc
     2281 attagacggg aaatccgtat cgccctcatc gtagaaaaca tcgtcccagg actcgcgcaa
     2341 ccggctcgga tcaacgaacc acatcagatc cgcgatgacg agggtgagcg gttggggcca
     2401 ccgttgcgcc agacgttcga gaaaagccgc tttcatcgga tcggtcaggt ggtggaacga
     2461 gtagacgcac agcaccttat taggtcgcac atccgcccct tcttcgcttt ctatgaggtt
     2521 ttcaaaagac ccgcggtaga accggacatt cgacaccccc gcctggtgtg ccgcttcccg
     2581 agcctgacga atcaagggct cgctgatatc gatgcccaca acctctttgc ataggggcgc
     2641 gtagaagaag gcccagcgac ctaaaccggt tccgatatcc agcagggtgt ttgtcttttg
     2701 gaggtccaac caatccgcgg ctttccggcc gacggtggta atgatcgaat ccgcttcgac
     2761 attcaactca acgagcggcg agctccgctc cagttttcca aactcttctt ccaaagcctc
     2821 gcgacgatag gtaggatttg ccataagacc tcccgtgatc cggttaagat agacgcacgg
     2881 aaggtattat atcaaaaaaa gggaagtcgg tttctctgtg ccgccggagc tccgagcact
     2941 tgggaacgcc gcgcttcagc gcggtgtgcc ttgaaaaaca aggttcgcac agagggcaca
     3001 gagaacacgg agagagcaag catcgcggcg taagcgcctc ttctcttcgt ggggccgact
     3061 tccagtcggc ctgcctctct gggaacacct gggaacgcca aactttagtt tggcttggtt
     3121 tcatcttttc attgggagtg tttccaagcc agcatcgcgg cgtaagcgcc ctttcttctc
     3181 gcgacggcgc gatacgcttt tgttctttat cttttccctt ttacgaaaga tcgtatcgcg
     3241 agacttcgtg ggcttccgaa gtcagcccgc tggttacccc gcgata
//
LOCUS       DAOPXN010000220         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_30079_length_3262_cov_5.509510, whole genome shotgun sequence.
ACCESSION   DAOPXN010000220 DAOPXN010000000
VERSION     DAOPXN010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30079_length_3262_cov_5.509510"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="PKM49_09430"
     CDS             <1..882
                     /locus_tag="PKM49_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83053.1"
                     /translation="PQVCREPFSDTQDQDFFVDFTNERTEDYYQSVRVKIKRVTFAYA
                     QAAQSFGEYLYQPTTYPNEKNLKLLWENQNWHISTLSPNWKDWSFAFKTPEIGWSSIG
                     FGADIQGFYESTPTRSFAVSGGPKIRLLFLDIVPYIGYKSQGSSETEGIDPFSTLTYG
                     LKTDFRYSPFSIKAGFTLAENPEYYCAVGLGPLLLSFGQKNSFSRPSGKDIWQQIEWD
                     WQEKGIETKIGFGRINQAFYVFSDIDVRFNDRWSLRASIKSGIDRILPLSAPTLGGEG
                     LSFSVGVEYRIPITITE"
     gene            917..1720
                     /locus_tag="PKM49_09435"
     CDS             917..1720
                     /locus_tag="PKM49_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNY83054.1"
                     /translation="MLDQEAPDPQFLWFIAEVKKRLNLDLSGYKPHRVKRRTDMLLRK
                     YNVGSYQEYMKLMMADEKKTEEFLDKMTINVTEFFRNPEKWEAIKRTYLPGLMKQSSR
                     IKIWSAGCSSGEEPYSVGILLEELRAPASASVLATDIDAGVLQKAKIGVYDERAFVST
                     SEDVLRRYFRKVDEIFYEVLQNVKNRVQFRNHNMLQDPFEKNLDMIICRNVVIYFEAE
                     TKNVLYQNFVDSLRVGGILFVGSTERIFNYRSLNLKVLEPFFYQKEGSV"
     gene            1723..2628
                     /gene="ftsY"
                     /locus_tag="PKM49_09440"
     CDS             1723..2628
                     /gene="ftsY"
                     /locus_tag="PKM49_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNY83055.1"
                     /translation="MGFFDRLKEGLKKTRDATFGKIKTLLSFSKIDAETLEQIEELLI
                     TSDISVSTTEKIIRGLKDKMKSEKSPTQALKRALLDLLEGDAYLRVPEKPPMVISIVG
                     VNGGGKTTTTGKLAKRFKEEKKEVVLAAADTFRAAAIDQLRVWAEERAGVPLIAHEPG
                     ADSAAVAYDAVSHALSKKKDIVLIDTAGRLHTKDNLMEELKKLHRVIKKIIPEAPHEV
                     LLVLDATTGQNGLAQAKKFLETVGVTGIVLTKLDGTAKGGIALSIKDELGLPIKYVGV
                     GEKIDDLRPFVAEDFVNALLEGEDE"
     gene            2667..>3262
                     /locus_tag="PKM49_09445"
     CDS             2667..>3262
                     /locus_tag="PKM49_09445"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNY83056.1"
                     /translation="MDRKVFWQKHYRCGLCGADFRSLRIFDHAIRVKQRDLFLHATYE
                     FPDPQHYAIIACPDCYYSAYDKDFEALPDKLNSQQIFQLQQALKKGKDTFHLNLSKER
                     ALQEVIDLYSLAVLTYLIAEDSYKMAQLYMKLGWFYLDDQNRENAAIAFSKSYQQFFE
                     TYERGRIPGEADGILFCLASIQILLNNVSEGFRWLERL"
BASE COUNT          868 a          762 c          877 g          755 t
ORIGIN      
        1 ccgcaggtat gtcgggaacc cttttctgat acgcaggatc aagatttctt cgtcgacttc
       61 acaaacgagc gaactgagga ctattaccaa agcgtgcgag tgaagatcaa acgggtgacc
      121 tttgcctatg cccaggccgc ccagtcgttt ggagagtacc tctatcagcc gaccacctat
      181 ccgaacgaga aaaaccttaa gttgctgtgg gagaatcaga attggcatat ttccaccctg
      241 agcccgaatt ggaaggattg gtcttttgcc tttaaaactc cggaaatagg atggtcctca
      301 atcgggttcg gagcggatat tcagggattc tatgaatcga cccccacacg atctttcgcc
      361 gtttcggggg gcccgaaaat acgtctgtta tttttggata tcgtccccta tatcggttac
      421 aaatcgcagg ggtcatcgga gaccgagggc atcgatccct tttcgaccct aacctatggc
      481 ttgaaaacgg attttcgata ctctcccttt tccattaaag cgggatttac gctagccgag
      541 aaccccgaat actattgcgc agtcggattg gggccattgc tactctcttt tggtcaaaag
      601 aacagctttt cgcgaccttc ggggaaggac atctggcagc agatcgaatg ggattggcag
      661 gaaaagggaa tcgaaacgaa gataggattc ggccgaatca accaagcttt ttatgttttt
      721 tctgatatcg atgttcgttt caatgaccgt tggtcgttac gggcgtccat caagagcgga
      781 atagacagga tattacccct gtccgcccct actttgggag gagaggggct gagtttttca
      841 gtgggagtgg aataccggat tccaataaca atcaccgaat gagtgaatag tatgatcaca
      901 gacataggag gaagagatgc tcgatcaaga agctcctgat cctcagtttc tttggtttat
      961 cgccgaagtg aaaaaacgat tgaacttgga cctctcgggc tacaaaccgc accgtgtcaa
     1021 gcgtcgaacg gacatgttgc tacggaagta caacgtcggc tcctaccaag agtatatgaa
     1081 actgatgatg gctgacgaaa agaagacgga agaatttttg gacaagatga cgatcaacgt
     1141 gacggagttc tttcgaaacc cagaaaaatg ggaggcgatt aagagaacgt atctacccgg
     1201 actgatgaaa cagagcagca ggataaaaat ctggagcgcc ggctgctcca gcggagaaga
     1261 gccctattcc gtgggtattt tgctcgaaga gcttcgagcc ccggcctcgg ccagcgtctt
     1321 ggccaccgat atcgatgcgg gtgttttgca gaaggcgaaa ataggggttt acgatgaacg
     1381 cgccttcgtc tcgacgtctg aagacgtcct caggcggtac tttcgaaaag tcgacgaaat
     1441 tttctatgaa gtgctgcaga acgtgaaaaa tcgggtacag ttccggaacc acaacatgct
     1501 acaggacccg ttcgagaaga acctggatat gatcatttgt cgaaacgtgg tgatctattt
     1561 tgaagcggaa accaaaaatg ttctctacca aaacttcgtc gattcgctgc gtgttggagg
     1621 tatcctgttc gtcggcagca ccgaaagaat ctttaactat cgatcgttga acctgaaggt
     1681 actggagccc ttcttctacc agaaagaggg ctcggtgtag ccatgggatt tttcgatcgt
     1741 ctgaaagaag ggctcaagaa gaccagggac gccaccttcg ggaagataaa aacgctgctc
     1801 tctttctcga agatagatgc cgaaacgttg gagcagatcg aggagctatt gatcaccagc
     1861 gacatcagcg tgagcacgac cgaaaagatt atccgagggc ttaaagacaa gatgaaatcg
     1921 gaaaaatccc cgacgcaggc attgaagaga gctctgttgg acctcttgga aggcgatgcc
     1981 tatttacggg taccggaaaa accgccgatg gtgatcagca tcgtcggggt caacgggggc
     2041 ggcaagacga caacaaccgg gaagctggcg aaacggttta aggaagaaaa aaaagaggtt
     2101 gttttagccg cggccgatac tttccgggca gccgcgatcg atcagcttcg cgtttgggcc
     2161 gaagaaaggg ccggggttcc tttgatcgcc cacgagcccg gggcggattc cgccgctgtg
     2221 gcgtatgacg cggtcagtca cgctctctcg aaaaagaagg atatcgtctt gatcgacacg
     2281 gccggaaggc tgcatacgaa agacaatctg atggaggaat tgaagaagct gcatcgtgtg
     2341 attaaaaaaa tcatcccgga ggctccccat gaagtgctgc tggttctaga cgcgacaact
     2401 ggtcaaaacg gattggcgca ggctaaaaag tttttggaaa cggtaggcgt taccggcatc
     2461 gtcttgacga aattggatgg gacggccaaa ggaggcatcg ccctatcaat caaagacgaa
     2521 ctgggtctcc ccatcaaata cgtcggagtc ggtgaaaaga tcgacgacct tcggcctttt
     2581 gtagcggagg acttcgtgaa cgcgctcttg gagggagaag acgaatagga gggtcttacg
     2641 cgcccgccca atttgtcgcc gactccgtgg acaggaaggt cttttggcaa aaacactacc
     2701 gttgtggcct ttgcggcgct gatttccgaa gcctgcggat attcgatcac gcgatacgag
     2761 tgaaacagcg cgacttgttt ttgcacgcca cctatgaatt tcctgatcct caacactacg
     2821 cgatcatcgc ttgccccgac tgttactact ccgcttatga taaagacttt gaagccttgc
     2881 cggacaagct gaattctcaa cagatttttc agctacagca ggctctgaag aaaggaaaag
     2941 ataccttcca cttgaacttg tccaaagaac gcgcgctcca ggaggtcatc gatctctaca
     3001 gtttggccgt cctgacgtat ctgatcgccg aagactcgta taaaatggcg cagttgtata
     3061 tgaaactggg atggttttat ctggatgacc aaaatcggga gaatgcggct atcgcgtttt
     3121 ctaaatccta tcagcagttt tttgagacct acgagcgtgg ccggattccg ggagaagccg
     3181 acggcatcct gttttgtttg gcttcgatac agatactctt aaacaacgtc tccgaggggt
     3241 ttcgatggct ggaaaggctc aa
//
LOCUS       DAOPXN010000221         3259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_30127_length_3259_cov_4.971910, whole genome shotgun sequence.
ACCESSION   DAOPXN010000221 DAOPXN010000000
VERSION     DAOPXN010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30127_length_3259_cov_4.971910"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..1094
                     /locus_tag="PKM49_09450"
     CDS             237..1094
                     /locus_tag="PKM49_09450"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HNY83057.1"
                     /translation="MKGTVLKKTRNYMIMFLGITLNAFGWTGFLLPHKIVGGGVNGIG
                     IMVNVVTGFPIGLFYLIVNIFLILWAVKSVGAHFGVKSIVGILGFSLMMSVLQPLTTN
                     PILSDAFMSTILGGFIGGFGLGLVFWQGGSTGGTDIIALILSKRFHSSPGKITLYINL
                     MIITSSYLLFRSWEMIIFGIVAMSVSSFSLDQFLEGTKMSAQIFVFSDHYQQIADNIT
                     MRMHRGVTVFDGIGWYTKEKRCALMILVTRKEVPEVLTIVKETDQEAFISVASVRSVY
                     GQGFGPLEF"
     gene            1611..2759
                     /locus_tag="PKM49_09455"
     CDS             1611..2759
                     /locus_tag="PKM49_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY83058.1"
                     /translation="MLIVKGAQIWKDRDLKRLDFSVGEGKIAAFSPSLLPGPQDEVLN
                     LSGHYVYPGLIDPHTHVGLLEETTGNFAGDDVNETTSPATAGVRAIDGINPLDPGIRN
                     ACNYGVTTVCVMPGSANVIGGEGVVIKTAGKSVDTMVVSSAKKIVKMAFGRNITWTWS
                     KKEKYPSTRLAVGAVLREKLNAAKNYMANPERVNTYEMEVLANLLKKEAIARIHVANL
                     EDIETIDRITREYEIDYVLDHASALHTNPEYAKTLRVPVILGPLNVAGKSFQSYDVTC
                     ASVKVLLEAGLLVSVMSDAPVIPLNLVRLQMFLIDRYRLNRADILDLFTENPAKTLGI
                     EKQTGSLDVGKDADFIVLDGDIFDYRSHVRSTYIYGKACGGEDYEMDS"
     gene            2743..>3259
                     /locus_tag="PKM49_09460"
     CDS             2743..>3259
                     /locus_tag="PKM49_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HNY83059.1"
                     /translation="MKWILKNAIIHPVTSETFRGSILIDKGKIAQINPAERKGTKIFD
                     GGGLHLFPGIVEEHCHIGTHGEKTNQTADQDTNEWSEPITPEMHALDGIKIYDFGFKM
                     ALANGITTLNIFPGSGSPITGIGVALKNDPSLRFEERILIKESGLKMAFGENPKSVHS
                     KTSKTIQTRMAT"
BASE COUNT          809 a          842 c          865 g          743 t
ORIGIN      
        1 aacgccgaac tttagttcgg caaagacgat cgcacaactt gttatgaaat cattatttaa
       61 gccacaagtt acgtgcacgc gcgggatgag tgatactctt tccctcgttt agtgttacaa
      121 tcgagccgga aaaaaaccgg agcgtactcc ggtgactcgg acgtgggccg aagacaccgc
      181 cgaagtcgct aagcgatgag agcggatcat cgtatctttc aatgggggcg aacgaaatga
      241 aaggaacggt acttaaaaaa accaggaatt atatgattat gtttctagga ataacgctca
      301 acgccttcgg gtggaccggc tttctcctac ctcataagat cgtcggaggc ggtgtgaacg
      361 ggatagggat catggtcaac gtcgtcaccg gtttcccgat cggcctattc tatctcatcg
      421 tgaacatatt tctgatcctc tgggcggtca aatccgtagg ggcgcacttc ggagtcaagt
      481 ccatcgtcgg cattctcggt ttctccttga tgatgtcggt tctccaaccc cttacgacga
      541 atcccatctt gtccgacgcc tttatgtcca ccattctggg cggttttatc ggcgggttcg
      601 gtttaggcct cgtcttctgg caagggggca gtacaggcgg cacggatatc atcgccttga
      661 ttttgagcaa gcggtttcac tccagcccgg gaaagatcac cctctacatc aacctcatga
      721 tcatcacatc ttcctatctg ctctttcgtt cttgggagat gatcatcttc gggatcgtcg
      781 cgatgagcgt ttcctccttc tcgctcgatc agtttttaga agggacgaag atgtcggcgc
      841 agatcttcgt gttttcggac cattaccagc agattgcgga taatattacg atgcggatgc
      901 atcgcggggt aacggtcttc gatggcatag gctggtacac gaaagagaag cgctgcgctt
      961 tgatgatcct ggtaacgaga aaggaagtcc cggaggtcct taccattgtg aaagagaccg
     1021 atcaggaagc cttcatctcc gtcgcgtccg tccggtccgt ctatggccaa gggttcgggc
     1081 cactggaatt ctagccgccc tctgggaact cccttgggaa cgccaatttt taagttggcg
     1141 tgtttgaggt ttttgggaag tcatcgcggt ggaaagaaaa caagggggga tcgcggggtc
     1201 ccttcggtta ccccgcgatt accccgttcg gtcatcgcgg cgtaagcgct tcttcttttc
     1261 gcgacggcgc gataggcttt tgtacgtgtc ttgaaaaaca gggttcgcac agagggcaca
     1321 gaggaaaaac tggcaaggga caaggctctc cctgaagaag aaaagacatg ccggactaaa
     1381 gtccggcgtt cccaagcgtc ccagggcgga tcgcgagact tcgtgggctt ccgcagtcag
     1441 cctgctagtt accccaagat tactttatac gggcatcgcg gtgtaaaaag agcaaaaggc
     1501 ggatcttctc ctctttctat tttttgttgc acctcgataa tatacccacg ccgtcgaatc
     1561 attctttgag gaggtttgtg gtaaaattgt tccgaaaggg gtgatagata ttgctgattg
     1621 taaaaggtgc gcagatttgg aaagaccgcg acttgaaacg gctagatttc tctgtgggag
     1681 aaggtaaaat agcggctttt tctccttctc ttctaccggg acctcaggat gaggttctca
     1741 acctgtcagg acactacgtc tatcccgggt taatcgatcc gcacacgcac gttggactat
     1801 tggaggaaac tacgggaaat ttcgccggag acgatgtgaa cgagaccacc agcccggcca
     1861 ccgcgggcgt acgcgcaata gacggcatca acccgctgga cccaggtata cgaaacgctt
     1921 gtaattatgg agtgacgacc gtttgtgtca tgcccggctc tgcgaacgtg atcggcggag
     1981 aaggcgtggt gatcaaaacc gcaggaaaat ccgtggatac tatggtcgtg agttccgcaa
     2041 aaaagatcgt caagatggcc ttcggtcgca atatcacctg gacctggtcg aaaaaggaaa
     2101 agtacccttc tacgcgtttg gctgtaggcg ccgttttacg ggaaaaactg aacgccgcaa
     2161 agaattatat ggccaacccc gaaagggtta acacgtacga gatggaagtc ttggcgaacc
     2221 tcttgaaaaa agaggcgatc gcacgcatcc acgtggcgaa cttagaagac atcgaaacca
     2281 tcgaccgcat cacgagagaa tacgagatcg actacgtgct tgaccacgcc tcggctttgc
     2341 acacgaaccc cgagtacgca aaaacgctga gagtgccggt gatactgggc cccttgaatg
     2401 tcgcgggcaa atccttccaa tcctatgacg tgacctgcgc ctccgtaaaa gttctcctcg
     2461 aggccggtct tctggtgtct gtgatgtccg acgcgccggt catcccgttg aacctcgtca
     2521 ggcttcagat gtttctcatc gaccgatacc ggctgaatcg cgccgatatc ctcgatctct
     2581 tcacggaaaa ccctgcgaag acgttgggca tcgaaaaaca aaccggctcc ttggacgtgg
     2641 gcaaagacgc tgatttcatc gtcttggatg gggatatttt cgactaccgc agtcacgtgc
     2701 gttcgaccta catctatgga aaggcctgtg gaggggaaga ctatgaaatg gattcttaaa
     2761 aacgcgatca tacatcccgt aacctcggag acctttcgcg gttcgatact gatcgacaag
     2821 ggcaagatcg cccaaatcaa ccctgccgaa agaaagggta cgaaaatatt cgacggggga
     2881 ggccttcacc tcttccccgg aatcgtcgaa gagcactgcc acatcgggac ccacggagaa
     2941 aaaaccaatc aaacggccga tcaggatacc aacgaatggt ccgaaccgat cacgccggag
     3001 atgcacgccc tcgatgggat aaaaatatac gatttcgggt tcaaaatggc tttggccaac
     3061 ggcatcacga ccctgaacat cttccccggg agcgggagcc ccatcaccgg catcggtgtc
     3121 gccctgaaga acgacccgtc cctccgtttt gaagaacgca ttctaatcaa agaatcggga
     3181 ttgaagatgg ctttcgggga gaaccccaaa agcgtccact ctaaaacctc caagaccata
     3241 cagactcgta tggcgacgg
//
LOCUS       DAOPXN010000222         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_30425_length_3235_cov_5.074214, whole genome shotgun sequence.
ACCESSION   DAOPXN010000222 DAOPXN010000000
VERSION     DAOPXN010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30425_length_3235_cov_5.074214"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PKM49_09465"
     CDS             complement(<1..397)
                     /locus_tag="PKM49_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNY83060.1"
                     /translation="MKEIAPGIIVYDNPFGGSNIVSVTTSEGTIIVDSSLFPSKAEQV
                     KTTVQRLLNSEVALVVNTHYHPDHTFGNSGFNAPLCCCKTSEEFFRKMDKTYIGYVIQ
                     KEPLLEKENLIIVPPSITFDREYKLSFGGL"
     gene            complement(1320..2585)
                     /locus_tag="PKM49_09470"
     CDS             complement(1320..2585)
                     /locus_tag="PKM49_09470"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNY83061.1"
                     /translation="MRQRVYGLVDCNNFFVSCERVFNPKLEGKPVVVLSNNDGCIVAR
                     SPEAKALGIPMGTAYFKIRKELDRHGVIAFSSNFALYGDMSRRILQTLSGFCPDIELY
                     SIDESFLSFRAFPLDHLEAFGKEIRDTVRRWTGIPVSIGWGPTRTLSKFANRLAKKHA
                     PYQGVFSAFSDETDSWMMRYPIDEIWGIGHRWGKKLHAKGIDTIYQLKTADLTTIRKM
                     MGVVGLRMVKELNGEPCITIAPPETRKSLIHSCSFGEPILDFEKLFEALAAHVSRGAE
                     KLRFEKCCANMMTIFVMTSRFAKEAYLGSQPIVFRRPTDNTIEMLRLAREGLKRIYLY
                     GYFYKKAGVYFSGIYPRARVQQSLFEDLESQQKEQQVVELVDRLNQNIRRNVVQFGSI
                     GTRKAHQDRKENVTPRYTTCWREIPTVRG"
BASE COUNT          760 a          857 c          808 g          810 t
ORIGIN      
        1 caaggccacc gaaagagagc ttgtactccc tatcgaaggt gatcgagggt gggacgatga
       61 tgaggttctc cttctccaaa aggggctcct tctggatcac gtagccgatg tatgttttgt
      121 ccatcttacg aaagaattct tccgaggttt tacagcaaca caagggggcg ttaaaccccg
      181 aattcccaaa ggtgtggtcg gggtggtagt gggtattgac gaccaaggcc acctcgctgt
      241 tgagcagcct ttgaaccgtc gtttttacct gttccgcttt ggaaggaaaa agcgaagaat
      301 cgacgataat cgtgccttcc gaagtcgtca ccgaaacgat atttgaacct ccaaaagggt
      361 tatcatacac gataataccg ggcgctatct ctttcaaaaa gatactcctc caaccgtcct
      421 gcgttgtaat ctaaaagtta tttaccacta tacgtttcta taaagagcgt gagaattcct
      481 ttctaaagaa ggcagaaaga cggtcggaac gtctgggaac tccgcagggc tgggagcaca
      541 gaaaacattg ttcgcacaga gtacacagag aacacagagg ggagattcaa actggggacg
      601 ctcgaggacg cttgggaacg ccaacttgta agttggcatg ttctctgaac ttcagttcgg
      661 catgtcttct atgttttcac tcctctctcc gtgccctctg tgttctctgt gcgcaacttt
      721 tttgtattct agagaacgcc tgggaacgcc ggactttagt ccgacatgct ttctatgttt
      781 tcatcctttc tctgtgccct ctgtgtcctc cgtgcgaacc cttcttttat cttctataag
      841 taacgccaat caaaaagatc ggtgttccca gtgtgcactt ctccgtgcca tccgtgccct
      901 ctgtgtgaaa ccccaaaacc ttccgttcgc acagagaaca cagaggggag attcaaactg
      961 gggacgcttg gggacgcttg gggacgcttg gggacgcttg ggaacgcttg ggaacgccaa
     1021 cttttaagtt ggtatgtttt cttcaactcc atccggtatg tcttctatgt tttcatcttt
     1081 ctccgtgccc tctgtgccct ccgtgcgaac ccgtctttcc tcttttttgg cgttcccagg
     1141 gccttcccaa tgtgcactct ccgtgttctc tgtaccctcc gtgcgaaccc gtctttcctc
     1201 ttttttggcg ttcccagggc cttcccagtg tgtactctcc gtgccctctg tgcgctccgt
     1261 gcgaaaccct tttttctttt tttggcgttc ccagagcggc gcccccgggc gtttacgttc
     1321 tatcccctta cggtggggat ttcccgccag caggtggtat aacgaggtgt cacgttttct
     1381 tttctatcct gatgtgcctt acgagtcccg atagaaccaa actgaacgac atttctacgg
     1441 atgttttggt tcaggcgatc gaccagttcg acaacctgct gctccttttg ttgagactcg
     1501 aggtcctcga acagggactg ttgaacccgg gcgcgggggt agataccgga gaagtacacg
     1561 ccggcttttt tatagaagta gccataaagg taaatacgtt tgagcccctc gcgcgccaag
     1621 cgcagcatct cgatcgtgtt atccgttggg cgacgaaaaa cgatcggttg gctgcccagg
     1681 taagcttctt tggcaaaacg agaggtcatc acgaagatcg tcatcatatt tgcgcaacac
     1741 ttttcaaaac gcagcttttc cgcaccgcga ctgacgtgag cggccaaggc ctcgaacaat
     1801 ttctcgaagt ccaagatcgg ctctccaaaa ctacaggagt ggatcaagct cttccgggtt
     1861 tcgggtgggg cgatcgtgat acagggctcg ccgttcaatt ccttcaccat ccgcaagccc
     1921 acgacgccca tcatctttcg tatagtcgtg agatcggcgg ttttgagttg gtaaatggta
     1981 tcgatcccct tcgcgtggag tttcttcccc cagcgatggc cgatccccca tatctcgtcg
     2041 atcgggtaac gcatcatcca ggaatcggtc tcatcggaaa aagcggagaa gacgccttgg
     2101 tacggggcgt gttttttcgc gaggcggttg gcaaatttgg aaagcgtacg ggttggcccc
     2161 caaccgatcg acacgggaat accggtccat cgacgaaccg tgtcacggat ctctttaccg
     2221 aaggcttcga ggtgatccag tggaaaagct cgaaaagaca aaaagctctc gtcgatcgaa
     2281 tacaactcga tatcggggca aaaccccgac aacgtttgca aaatccttcg ggacatatcg
     2341 ccgtagagcg cgaagtttga ggagaacgcg ataaccccgt gccggtcgag ctctttacga
     2401 atcttgaaat aggcggttcc cataggaata cccaacgctt tagcctctgg ggaacgcgcc
     2461 acgatacagc cgtcgttgtt agaaagaacc acgaccggct tcccctccag tttcggattg
     2521 aaaacccgtt cgcaagagac gaaaaaattg ttgcaatcca ctaaaccata gacccgttga
     2581 cgcatacgcc caccgaccat atcccaacca ggagaatcct gtgcaatact taacgcttaa
     2641 cgataccaaa aaactgtgaa aaaagcaacc ggcccgagaa cgcttaggaa cgctctggaa
     2701 ggcgcgctgg gaacgccttc aggacgccgc cagagcggcg gactttagtc tggcatgtct
     2761 ttttgttttg ggaacttctg ggaacgccaa cttttaagtt ggcatgtttc acaagataaa
     2821 acaggatttc gcacagagaa cacagagtac acagagaacg cagagggaga ttcacgctag
     2881 gagcggcgga gaacgccaca cttcagtacg gcatagggat ttcttgggtc atcgcggggt
     2941 aaccaacgga accccgcgat ccgctctttc gcattatcgg tagaaaggat tcagaaactg
     3001 gaaaaaagcg cagatgtgta gcgcgccttt gcggaaagaa aaatcgctta cgccgcgata
     3061 tatgttctct ccgcgttctc cgtgccctct gtgcgaaccc ttcttttatc ttctaaaagt
     3121 caagccaacg ccttaagtac gtagatcccg ttcaacacga ggagctcttt cgcggaagac
     3181 gtgtatccgc tttgggagaa cgccgcgaaa aaagttttcg ggcttttttc gttga
//
LOCUS       DAOPXN010000223         3208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_30795_length_3208_cov_5.716461, whole genome shotgun sequence.
ACCESSION   DAOPXN010000223 DAOPXN010000000
VERSION     DAOPXN010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30795_length_3208_cov_5.716461"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="glpX"
                     /locus_tag="PKM49_09475"
     CDS             <1..870
                     /gene="glpX"
                     /locus_tag="PKM49_09475"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNY83062.1"
                     /translation="TLVIGEGEKDEAPMLYTGEEVGSWEPTAPEMDIAVDPIDGTRLV
                     AYGLPGAIAVIAVAERGKICPLPTFYSYKLAVGPELKGQLDMNAPIRENLRVAAATLN
                     IKIGDLNVVVLNRDRHQGLIKEIRSVGARIKLITDGDIAGAIATATPDSEADIYVGIG
                     GSPEAILAAAALKTLGGELQLKLWPSSEQEKSALAEKGWDFEKVYHTNDLVSGDHVLF
                     AATGVTTGDFLNGVRFVKGKAITESLAIRSPSNTIRKIITYHDLNTKTLRVKSTEGDE
                     RFIELNRKVRMGK"
     gene            complement(884..2959)
                     /locus_tag="PKM49_09480"
     CDS             complement(884..2959)
                     /locus_tag="PKM49_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNY83063.1"
                     /translation="MRFRFVLFFLWIATVTMSIATLQVRFVGDLSYVPFEILESDGQA
                     KGYFVDFWRLAGKKAGFSVTYELCQWKDALAGFEKGDFDVVGGIFYSEQRSKRYLFSK
                     PFLEIPTSVFYRSHIDSIVSLRDLQKYRVGVVEQDYSQEYLERNAGKANLVLYPTTEA
                     LVLAAIEGEISVFLCDEPVALYWLKAYSKAQDFKISPPFYKNFVYAVVRPDQKELLDL
                     LNRGIDLITEEEQSNLESTWFGVPKTHTIPWALILVLSLIAFGVLISVFIWNSVLRRR
                     IEAAIRTIKEQNERLSRQNLALTENEQQFEGVNQQLLATTEDLEKTLLQVEGLNYTMF
                     ELIELSKLLSEAAQGKDQTFFEKLLNTTLTVIPSADYGSVSLFEGEDWRFVYAIGHDL
                     ANLKTLRLKKSQFTLEGKISVVEDIISDNRPKMNATELEKLAQFTKPIAHSIVIGLFI
                     GESLVGGISIDISAYSDKRFTQRDTEVAQALSNLASGFLAMQRYTIAQGKFQKDLLMG
                     MIKILEIYDPHTKGHSENVASLSARFAEELGISSDLIQRIYWAGLVHDIGKILVSREL
                     LGKMGPLTSSEYQIIQNHPVWGAEVLQSSDELSDIVLYIRHHHERWDGNGYPNRLEGE
                     SIPFVSRIISLADSYDAMISDRPYRKKKTVECALKDILNNAGKQFDPSLAKAFCAMIE
                     GKSAKRSVI"
BASE COUNT          815 a          831 c          829 g          733 t
ORIGIN      
        1 acccttgtga tcggcgaagg ggaaaaagac gaggctccaa tgctctatac cggcgaggaa
       61 gtggggagct gggaaccgac tgctcccgag atggatatag cggtcgaccc gattgacggg
      121 acgcgactcg tagcctacgg gttgcccggc gcgatcgccg tgatcgcggt ggccgagaga
      181 gggaagattt gtcccttacc gaccttctat tcttacaagt tggccgtcgg tcccgagttg
      241 aaaggtcaac tggatatgaa tgcccccatt cgagagaacc tcagggtagc cgcggccaca
      301 ctgaacatca aaatagggga cttgaacgtg gtcgttctca accgagatcg gcatcaaggg
      361 ctgatcaagg aaatcagatc tgtcggggct cgtatcaaac tcatcaccga tggcgacatc
      421 gccggcgcca tcgcgaccgc gacaccggac agcgaagccg atatctacgt cggaatcggc
      481 ggttctccgg aggccatact cgccgccgca gccctaaaaa cgctgggtgg agagctgcag
      541 ctcaaactat ggccctcgtc cgaacaagaa aagagcgccc tcgcagaaaa agggtgggat
      601 tttgagaagg tatatcacac caacgatctc gtcagcgggg atcacgtgct gtttgccgcg
      661 acaggtgtca cgaccggtga ttttctcaac ggcgtacggt tcgtaaaagg taaggccatc
      721 acagaatcgc tggccatccg ctctcccagc aatacgatcc gaaagatcat cacttaccat
      781 gacctgaaca ccaaaacctt acgggtgaaa tcgacagagg gtgacgaaag gttcatcgag
      841 ctcaaccgca aagtgcggat gggcaagtaa acggatcgac ccgttatatt accgagcgtt
      901 ttgccgactt cccctcgatc atcgcgcaaa aggccttcgc caaagagggg tcaaattgtt
      961 tgcccgcgtt gttcaaaata tctttcaagg cgcattcgac agtctttttt ttacgatacg
     1021 ggcggtccga aatcatcgcg tcataggaat cggccagcga aataatccga gagacgaaag
     1081 gaatggactc tccttctagc cgattggggt atccgttccc gtcccaccgt tcgtggtggt
     1141 gccggatgta gagaacgata tcgctcaatt catccgaact ttgaagaacc tctgcgcccc
     1201 ataccggatg attctgtatg atttgatatt ccgatgaggt taacggcccc attttaccca
     1261 ggagctctct ggacaccagg atttttccga tatcgtgaac caaacccgcc caatagatac
     1321 gctggatcaa gtccgatgag atacccaatt cctcggcgaa acgcgcagat agggaggcga
     1381 cgttttcgga atgacctttc gtatggggat cgtagatctc caatatcttg atcataccca
     1441 tcaacaaatc tttctgaaac ttcccttgag cgatcgtgta ccgttgcatc gcgagaaaac
     1501 cggaagccaa attcgacagg gcttgggcga cctcggtgtc cctttgggtg aaccgtttgt
     1561 cggaataggc agagatgtcg atgctgatcc cgccgacgag agattctccg atgaacaagc
     1621 cgatcacgat ggaatgcgcg atgggcttcg tgaattgggc aagtttttcg agctcggtcg
     1681 cattcatctt gggccggttg tccgagatga tgtcttcgac gacagagatc ttcccttcca
     1741 gggtgaactg ggactttttc agtcttaacg tctttaggtt cgccaggtca tggcctatcg
     1801 cgtaaacgaa ccgccaatcc tctccctcaa agagagaaac gctcccgtaa tccgcgctcg
     1861 gtatcaccgt caacgtggtg ttcagcagct tttcgaaaaa ggtttggtct tttccctgcg
     1921 ccgcctcgga cagcagtttc gaaagctcta tcagttcgaa catcgtgtag tttaatccct
     1981 ccacttgcag gagggtcttt tccaaatcct ctgtggtggc caggagctgt tggttaacgc
     2041 cttcgaattg ttgctcgttt tccgttaagg ccaagttttg gcgggatagc cgctcgtttt
     2101 gctccttgat tgtccttatc gccgcttcga ttcgccgacg caagacggag ttccaaatga
     2161 acacggagat gagcaccccg aaggctatta gggagaggac aagaatcaaa gcccagggta
     2221 tcgtatgcgt tttcgggact ccaaaccaag tactttcgag gttcgattgc tcttcttcgg
     2281 taatgagatc gattccacga ttcaggagat cgagcaattc cttttgatcc ggcctaacga
     2341 cggcgtacac gaaattttta tagaaaggag gagagatttt gaagtcctga gccttagaat
     2401 atgcttttag ccaatacaaa gcgacgggct cgtcacagag gaagacgctg atctcccctt
     2461 cgatcgcggc caaaaccaac gcttccgtcg tcgggtacag gacaagattg gctttgcccg
     2521 cattccgttc caggtactct tgcgaatagt cttgctccac cacgccgacc cggtattttt
     2581 ggaggtctcg aagactcacg atcgagtcga tgtgggagcg gtaaaagacc gaggtaggta
     2641 tttctaagaa aggtttcgag aacaaatacc tcttcgaccg ttgctcggaa taaaaaatcc
     2701 ctccgaccac atcgaaatcc cctttctcga aacccgcgag cgcgtctttc cactggcaga
     2761 gttcataggt caccgagaac ccggcttttt ttccggctaa gcgccaaaaa tcgacgaagt
     2821 accccttggc ctgcccatcg ctttcgagta tctcgaaggg tacgtaagat aggtccccca
     2881 cgaagcgaac ctgaagcgtg gctatactca tcgtaacagt ggctatccaa aggaaaaaca
     2941 atacaaagcg aaatcgcata acgccctctc gtcatttttt tctacacgaa gtcggaaatg
     3001 cctcagagaa ttataacata ccgaagaaaa gaaaggtaac gggggtgtgt gtcaagccat
     3061 ggggtgtaat caagaaacaa ggccagaatc gattgtacat atcaaaaaaa cagggttcac
     3121 acggagggca cagagaaaac agagaaaaga ttcttgtgaa cgccctggga acgccgaact
     3181 ttagttcggc acagtttttg tgaacgcc
//
LOCUS       DAOPXN010000224         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_32888_length_3062_cov_4.690722, whole genome shotgun sequence.
ACCESSION   DAOPXN010000224 DAOPXN010000000
VERSION     DAOPXN010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32888_length_3062_cov_4.690722"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..1813)
                     /locus_tag="PKM49_09485"
     CDS             complement(239..1813)
                     /locus_tag="PKM49_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNY83064.1"
                     /translation="MRKAQCIGLLVIIVMTGMAFSTTLFELAGGGKASEVDKALALGS
                     KIDERDRDGRTPLMYAAWNNTDVEVVMILLRRGANVNLQDANGWSPLMFAARYNTNVE
                     VLKVLLQAKADTRARNHHGLNPLALACGQENPNPEFVRALLDAGADVTITDTEGWTPL
                     MVSVWYNTDPRIVRLLVDAKADVNSRSVDGSTPLMLASAKETGNLGVIAALLDAGAKV
                     DTQNNNGISALMFACANPHPNTEVITALIGAGAAIHQRSNDGFSALMFAAKKSQNPEV
                     LRLLVNVGADVQARDNDGWTPLMLASWYNTSPEIIKALIDSGAKTNDGGDTGFTPLML
                     AANREDSHIEIIKTLLDAQADANRENQEGLTALMFAAAKENPDLVVIEALIANGAKVN
                     VGDHDGWTPLMFGAWFNESPEALDVLLEADAAINGRSLTGLTPLMFAARYNPNSQIAK
                     RLLEAGADVDATDESGMTALMYAAKETRNPEILTLLLAAGAEGGTQDASGKTAYDYAL
                     ANPVLRETSELRALGK"
     gene            complement(1918..2331)
                     /locus_tag="PKM49_09490"
     CDS             complement(1918..2331)
                     /locus_tag="PKM49_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HNY83065.1"
                     /translation="MKEMISHCGYRCDLCLAYKPNIEADPENPRRLSGGWRRYFGLRI
                     PPENIICDGCLAKDPQLIDKNCPVRLCVIEKGISTCAECTAYICEKLEELLVVFEDIR
                     KQREDPIPDEDRRLFIFPYENRDRLEILRRSSSEK"
     gene            complement(2567..2794)
                     /locus_tag="PKM49_09495"
     CDS             complement(2567..2794)
                     /locus_tag="PKM49_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83066.1"
                     /translation="MNIALGPDSETYDPIKQDVTPPVSLNIRNVKWFKFDRWYNFCGM
                     RAYRTRNERIIVGGSRPDCGMHVKTAYHQKR"
BASE COUNT          625 a          833 c          763 g          841 t
ORIGIN      
        1 ttgagaaagt ggcttttatc gccgtttgta gcacttttag tcgaaagttg ggataggagt
       61 acctgggaac accgaacttt agttcagcat cccttggttt tgtttttcgg cttttagctt
      121 gttgggaacg ccgaacttta gttcagcttg ggtttttggg agtgcagaac tttcgtccgg
      181 cgttcccaag ctgacgctca cagaggagca ctcacgaaaa agcacgacta tcgtttcgtc
      241 atttacccag cgcccgtagt tccgacgttt cgcgtaaaac ggggttcgcc agggcgtaat
      301 cgtaggctgt cttcccggag gcgtcctggg ttccgccctc cgcgccggcg gcgagtagaa
      361 gagtcaggat ttcaggattg cgtgtttctt tcgccgcgta catcaatgcg gtcatacccg
      421 attcatcagt cgcgtccacg tcagcccccg cttcgagcaa cctcttcgct atctgtgagt
      481 tcggattgta cctggcggcg aacatcagcg gcgtaagccc ggtaaggctt cggccgttaa
      541 tcgccgcgtc ggcctcgagt agcacatcga gggcttcggg gctctcgttg aaccaggctc
      601 cgaacattaa cggggtccaa ccgtcgtggt ctcctacgtt gaccttcgcg ccgttggcga
      661 tgagcgcttc gataaccacc aggtccgggt tttccttcgc ggctgcgaac atcagggcgg
      721 tcaacccttc ttgattttct ctgttcgcat cggcttgagc gtctaggagc gtttttatta
      781 tttcgatatg agagtcctct cgattggcag ccagcattaa aggcgtgaag cccgtgtctc
      841 cgccgtcgtt cgtctttgct ccggagtcga tgagcgcttt gatgatctcc ggagaggtgt
      901 tataccatga ggcgagcatt aaaggcgtcc aaccgtcgtt atctctcgct tgtacgtccg
      961 ctccgacatt gacgagtaga cgaaggactt cagggttttg gctttttttg gcagcgaaca
     1021 tcaacgcgga aaaaccgtcg ttgcttcgtt gatggatggc cgccccggcg ccgatcaacg
     1081 cggtgatcac ctccgtattg gggtgcggat tcgcgcacgc gaacatcagc gcggatatcc
     1141 cgttgttatt ctgcgtatcc accttcgcac ccgcatcgag aagggcagcg atgacgccga
     1201 gattgcccgt ctccttcgca gaggccagca tcaaaggcgt agaaccgtcg acgctccggg
     1261 aattcacatc ggctttcgca tcgacgagga gccgcacgat tctcggatcg gtgttatacc
     1321 aaacgctcac catcaacggg gtccatcctt ctgtatccgt aatcgtcacg tctgcgcctg
     1381 cgtctagcaa ggccctgaca aattccgggt tcgggttttc ctgcccgcag gccaacgcca
     1441 acgggttcaa gccgtggtgg ttgcgggcac gggtatccgc tttcgcctgt aatagaacct
     1501 tcagcacttc aacgttcgta ttgtatcgcg ccgcgaacat cagcgggctc catccgttcg
     1561 catcttggag gttgacattc gcgccccgtc gtagcaagat cataaccact tcgacgtctg
     1621 tgttgttcca cgccgcatac atcagaggcg ttcttccgtc ccggtctctt tcgtctattt
     1681 tcgatcccag agcgagcgct ttgtctacct ccgaagcttt cccgccccca gccaactcga
     1741 acaacgtggt agaaaacgcc attcctgtca tcacgatgat gactaacagt ccaatgcatt
     1801 gtgcttttct cacggtgata tcgtcctttc tttatgatcg ttcgaccaac gaatatactt
     1861 atagctcgca ttcgcctact tcttgacgtg atatgtggaa tgcgcttttg cctcgggtta
     1921 tttttccgaa gaggaacgtc gtagaatctc caaccggtct cggttttcat atgggaaaat
     1981 aaagagccgc cgatcctcat ctggaatagg gtcttcccgc tgtttgcgaa tatcctcgaa
     2041 aacgaccaag agttcttcaa gcttttcgca gatatatgcg gtgcattccg cgcaggtcga
     2101 gatacctttt tcgataacgc acagtcggac cggacagttt ttatcgataa gttgcgggtc
     2161 tttcgccagg cacccgtcgc aaatgatgtt ttcgggtggg atacgtaacc cgaaatatcg
     2221 acgccaaccg ccgcttaatc gtctcggatt ttccggatcc gcttcgatat tgggtttata
     2281 ggctaaacag aggtcacaac ggtatccgca atgcgatatc atttctttca tcgtcatcat
     2341 tcctttcttt taggtgttac gcttctttga acacgaaaac cctctctctg cgaggggttc
     2401 aagcggttca tccgggaata ctccacgatg tctccgtttt tgcgaaataa gctcttctca
     2461 attctcggta gcaaatgcgt gggtatcatt tttttcgctc ggtctgttat tttcttcttt
     2521 cgattactgg cctaattacc cctgatccat agtctacacc gctgttttat ctcttctgat
     2581 gatacgcggt tttcacgtgc attccacagt cgggcctact cccaccgacg attatacgct
     2641 cattacgcgt tcgataggcg cgcatcccac agaaattata ccatctatcg aacttaaacc
     2701 atttcacgtt ccggatgttt aaagaaaccg ggggtgttac gtcctgtttt atcggatcgt
     2761 acgtttcgga atccggtcct agcgcgatgt tcattaacac ttctcccggc ggtatacccg
     2821 gttcctcgag catccataag cccgaattcc gatttttttg atggcaggag atgggcgttt
     2881 cacaccggct ttatttgata taatataata gtcgtgtcct cgctaaaaga ctcgagataa
     2941 ccctcgggcg cacagcaagg ttggtttcgc gtggatgttc tagaaagagt ccggaatctt
     3001 tctttccagg gtactaaaag aagagcgaga atacgccctt ctgccagggg gccttaagga
     3061 aa
//
LOCUS       DAOPXN010000225         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33134_length_3047_cov_4.600602, whole genome shotgun sequence.
ACCESSION   DAOPXN010000225 DAOPXN010000000
VERSION     DAOPXN010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33134_length_3047_cov_4.600602"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1676)
                     /gene="rlmD"
                     /locus_tag="PKM49_09500"
     CDS             complement(291..1676)
                     /gene="rlmD"
                     /locus_tag="PKM49_09500"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNY83067.1"
                     /translation="MILAYENEITIGSAYQIVIEKLVFGGYGLGYIEGSVPVFIESAY
                     PGEIVRATIASKSRGAFFGTVQDWIYRVPERNGTVCSVFNQCGSCEWLDYDYQSQLKA
                     KKELLREQFLRIGKLDIMPVLFDVIPSKPYGYRNKMEYAFRNVSGAVVLGLKRKKSHN
                     VVPTRGCKIVPEGFERIRKTVESFANRSKTPTPVYDRSTKRGALKDLVVRTNADLTRW
                     MVIVVTDSPSFKGAADFVKTLREECPFVTSIIHLKNSSDKVVLRGAQSTLYGEGVLAE
                     TLGWFQYHVPSTAFFQSNRALLEPLLELVKTELELEKDENLLDLYAGIGFFSIYLAPL
                     VKYVLAVEGNSVAAKAIVKNMRLNQLSNVRAIQADIEKADLTKVLDRKVSKVILDPPR
                     AGISTATAQGLAALAPEKILYLSCDPATLARDIAILVKNGYFLRKVQPIDMFPHTSHV
                     ETAVSMSRVKD"
     gene            complement(1673..2089)
                     /locus_tag="PKM49_09505"
     CDS             complement(1673..2089)
                     /locus_tag="PKM49_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNY83068.1"
                     /translation="MKSYRKELWFETNQRRAFINITALLEACLKESGVQEGLCLCNAM
                     HITASVFINDDENGLHQDFETWLERLAPEKPHNQYRHNSFEDNADAHLKRQVMGREVV
                     IAITEGRFDFGPWEQVFYGEFDGKRKKRVLVKIIGE"
     gene            complement(2043..>3047)
                     /locus_tag="PKM49_09510"
     CDS             complement(2043..>3047)
                     /locus_tag="PKM49_09510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNY83069.1"
                     /translation="SFFGFRNLVHSFVNLIFTWVYSMILNRWPNRFGIQWVLGISVTA
                     GFISLLVLSRQHEPPIKEIQSVSYLKLFRSNKDYQKLLVFGGCWNFAILFSAPFFSYH
                     QLENLKVNFAWIGYLSMLTSLITMAFYWIWGRIADRIGHKNILRVGIAIACFIAIMWF
                     FMTPETMGTLLWVDAVTSALAWSAINLAVFTLPMLVGGESAIVLVAFFGAVNGLFGFI
                     GSLVGGLLSRWLVPLTFTMGELNMQGIQFLFLGSGVMRIGCLFLLKRVSVVGHIRLRT
                     ALFETMTNITRRGAKEHTEPGLASAIVETIDQKEENLGNNRSGHEKLSERTVVRDQST
                     "
BASE COUNT          713 a          825 c          765 g          744 t
ORIGIN      
        1 tctctgtgcc ctctgtgcga accctgtttt tcaatgcagg tacaaaagcg tatcgcgccg
       61 tcgcgaaaag aaagggcgct tacgccgcga tgcctgttct ctctgtgccc tctgtgcgaa
      121 ccctgttttt caatgcaggt acaaaagcgt atcgcgccgt cgcgaagaga aggggcgctt
      181 acgccgcgat aaactgtgtt ctctgtgcga aaccctgttt tgttctttaa aacatgccga
      241 actgaagtcc ggcgttctca gtcttgcact cacaggatgt ggagttttgt tcagtctttt
      301 acgcgggaca tcgacacggc cgtctcgacg tgtgacgtgt ggggaaacat atcgatgggt
      361 tgaacctttc gtagaaagta gccgtttttg accagtatcg caatgtctcg cgccaatgtg
      421 gccggatcgc aagacaggta caggattttt tcaggggcca aggctgccaa accttgggcg
      481 gtcgccgtgg aaatcccagc gcgcggcgga tccaagatca ctttggagac cttacggtcc
      541 aacacttttg tcaggtccgc cttttctata tccgcttgta tcgcgcgtac gttgctcagt
      601 tgattcaaac gcatattctt cacaatagct tttgcggcca ccgagttgcc ttcgaccgcc
      661 aatacgtatt tgacaagggg ggctaagtag atggagaaga agccgatccc agcgtataaa
      721 tcgagcaggt tttcatcctt ttcgagctcg agctcagttt ttacgagttc cagcaacggt
      781 tccaacaaag cacggttgct ctggaaaaag gcggtcgatg ggacgtggta ctgaaaccag
      841 cctagggtct cggctaacac accttcgcca tagagcgtcg attgcgcccc ccttaacacc
      901 accttatccg agctgttctt caaatggatg atcgaggtca cgaaagggca ctcctcccga
      961 agcgttttga cgaagtcggc ggcgcctttg aaagagggtg aatcggtgac gacgatcacc
     1021 atccaacgcg tcaggtccgc gtttgttcgt acgacgaggt ctttgagcgc gccccttttc
     1081 gtcgagcggt cgtataccgg cgtgggggtc ttagaacggt tcgcgaagct ttccacggtt
     1141 ttacgtattc gttcgaagcc ctcgggtacg atcttgcatc cgcgtgtcgg cacgacgtta
     1201 tggctcttct tgcgcttcaa ccccaaaaca actgcaccgc tcacgttgcg gaaggcgtac
     1261 tccatcttgt tccgataccc atacggtttt gaaggaatga cgtcgaagag gacaggcatg
     1321 atatccagtt tcccgatgcg tagaaactgc tcccgaagga gctccttttt cgctttcaat
     1381 tggctttgat agtcgtaatc caaccactcg caactgccac attggttaaa aacggaacaa
     1441 accgtcccat ttctttctgg gactctgtat atccaatctt ggaccgtgcc aaaaaacgcg
     1501 ccgcggctct tcgaagcgat cgtcgctcga acgatctcgc cagggtaagc actttcgatg
     1561 aaaaccggga cgctgccctc gatatatccc aaaccgtagc ctccgaagac gagcttttcg
     1621 atgacgatct gataggcgct cccgatggtt atctcatttt cgtaagccaa aatcattcac
     1681 cgatgatctt cactaagacc cgtttcttac gctttccgtc aaactccccg tagaaaacct
     1741 gctcccacgg gccgaagtca aaacggcctt ccgtgatcgc gatgaccact tcgcgaccca
     1801 tcacctgtct ttttaggtgg gcgtcggcgt tgtcctcaaa gctattatga cggtactggt
     1861 tgtggggttt ttccggcgcc agtcgttcca accaagtttc aaaatcctga tgtaatccgt
     1921 tttcgtcgtc gttgatgaac acgctggccg tgatatgcat cgcgttgcac aaacagaggc
     1981 cttcttgtac gccactttct tttaagcacg cttctaatag tgccgtgata ttgatgaagg
     2041 ccctacgttg attggtctcg aaccacagtt ctttccgata acttttcatg accactccta
     2101 ttgttcccga gattctcttc cttctggtcg attgtttcca cgatcgcgga cgcgagaccg
     2161 ggttcggtgt gctcctttgc gccgcgtcgg gtgatattgg tcatcgtctc gaacagcgcc
     2221 gttcgcaagc ggatatgccc gacgaccgag acccgtttca gtaagaacag gcacccgata
     2281 cgcatcaccc cactaccgag gaatagaaat tggatgccct gcatgtttag ctcccccatc
     2341 gtgaaggtta aggggacaag ccaacgtgag agcaagccgc caaccagcga accgatgaat
     2401 ccgaatagtc cgttcaccgc tccaaagaag gctaccaaaa cgatcgcgga ttctccgcca
     2461 accaacatag gcagcgtaaa gaccgccagg ttaatcgccg accacgccaa cgcgcttgtc
     2521 accgcatcca cccacaggag cgttcccata gtctccggag tcataaagaa ccacattatg
     2581 gcgatgaagc acgcgatcgc aattccgact cttaaaatgt ttttatgccc gatcctgtca
     2641 gcgatccgtc cccaaatcca gtagaaggcc atcgtgataa ggctggtgag cattgatagg
     2701 tatccgatcc aagcgaaatt gaccttcagg ttttccagct gatggtatga aaaaaacggc
     2761 gcggaaaaaa ggatggcgaa gttccaacat ccgccgaaga ccaagagctt ctgatagtct
     2821 ttgttggagc ggaagagctt gaggtacgat acggattgga tttctttgat cgggggttcg
     2881 tgttgtcgcg ataagacgag taggctgata aagccagccg taacggagat ccccaacacc
     2941 cattggatgc cgaaacggtt cggccagcgg tttaggatca tcgaatagac ccaggtgaag
     3001 atgaggttta cgaacgagtg aaccaaattt cggaatccaa agaagct
//
LOCUS       DAOPXN010000226         3036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33296_length_3036_cov_4.723583, whole genome shotgun sequence.
ACCESSION   DAOPXN010000226 DAOPXN010000000
VERSION     DAOPXN010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33296_length_3036_cov_4.723583"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..2892)
                     /locus_tag="PKM49_09515"
     CDS             complement(109..2892)
                     /locus_tag="PKM49_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83070.1"
                     /translation="MVKNIALSLAISVAILFIVGFAMLVTSCLNTPVPMTWEPPRIEN
                     PFPEDGSLDQPFNLVLRWSAVPGNPPGTPIPSATITHYDLRLLLDNEGAAEHTFLLQE
                     PECEITDLAEHSLYRWIVRAYQSDGQSADSPEWTFKTKKREYGIPQISLIFPENGASD
                     QATELILTWAAAPGDLLETFPRLVEISEFHVHITEDGAEEPTEFATVNLPCFLISGLN
                     YGTSYRWYVVAKQSDGKTATSTEWKFQTAPRKYAEPSLRLLGPEDGKTNMDTQVTFVW
                     DGKHGDLCNIEARADSPLEYDLFLRPDGQPYGIPYHTRKCEKTVSSLLYGTTYRWKVV
                     TYQPDGQSAESEERSFRTKAATYAPPILALLYPENGQTDCATAITLSWQATPGHQTNH
                     SARVAFITEYELYFYETGESLPLPIALTKNSHSLHSLNYHQEYRWKVIAHQSDGQRVE
                     SVERCFMTQGESFYPPEIRLMFPLNAETDVATKVRLSWEATPGGRSNLSAREPPTLTG
                     FSLYFAKEGGPFDPPIAIEDPETREWEISGLDRDTAYHWAVEAHQSDGQSAISPTSRF
                     RTIPPPIALYDSTGAFLAYFDLLSQSISGAPEKGLIVVRGGTVLRNEEAELKISGKEI
                     TILGQPDNPFTIDMEGRGRVFHITNGASVTLENIEITGGSIDGKKDGGAIYLYGKDSR
                     LTLRRAKIVGNRSGSQGGGIHMFSGALTMVDSEVINNEAGTSGGGIDARAENLRIENC
                     LISDNRAKTAGGGLYIACTATIARSVVSNNTATRNGGGIYLANGALVAEETAIVMNRC
                     EDTADFGDGGGIYVYYNGALYAEGLVIDGNFAKQNGGGIAVNRGTIAANRLTVSSNTA
                     LYEGGGGIFRHSSGGTLTTNESVWAQAGDRQNEFSVSSEGEVRTDGTRLPEDPVHVYR
                     NMSGSESSQQMKW"
BASE COUNT          627 a          827 c          798 g          784 t
ORIGIN      
        1 tttcagcgtt gttttttctt attttttccc tttttctcca aaaccggtcg aaagtttggt
       61 agggcttttt cggttattct tcggcagaag cccttacgtg accttcgatc accatttcat
      121 ttgctgactc gattccgagc cggacatatt tcgatacacg tgtacggggt cttctggcaa
      181 tcgggtcccg tcggttctca cttccccttc ggaggatacg gaaaactcgt tttgacggtc
      241 tccggcctgc gcccaaacac tctcgttggt ggtcaaggta ccccccgaag agtggcgaaa
      301 aatccccccg cccccttcgt aaagggcggt gttagaggat acggttaggc ggttcgccgc
      361 tatcgttccc cgattcacag ctatcccacc cccgttttgc ttggcgaagt tcccgtctat
      421 cacaaggcct tcagcgtaca aggccccgtt atagtataca tagatgcccc cgccgtcacc
      481 aaaatcagcg gtatcctcac aacgattcat cacgatagcc gtctcttccg caacgagcgc
      541 accgttcgca aggtagattc cccctccgtt tcgggttgcc gtattgttgg agaccaccga
      601 tcgcgctatc gtcgcagtac aggcgatata gaggcctcca ccggcggttt tggcacgatt
      661 gtccgaaatc aggcagtttt cgattcgtag gttttcggcg cgagcatcga tcccgcctcc
      721 ggatgttccg gcctcgttgt ttattacctc gctatcgacc atcgttaggg cgccagaaaa
      781 catatggatt cctccgccct gacttccaga acgatttcca acgatcttcg cgcgtctgag
      841 ggttaagcga gaatccttcc cgtagaggta gatagccccg ccatcttttt tcccgtcgat
      901 gctgccgccg gttatttcga tgttctccag ggttacggac gccccattcg ttatatggaa
      961 gacgcgccct cttccttcca tatcgatcgt gaatgggttg tccggttgcc ccaagatcgt
     1021 gatctccttc ccggaaattt ttagttctgc ttcttcgttt ctcagaaccg ttcctcccct
     1081 cacgacgatc aagcctttct cgggcgcgcc tgaaatcgat tggctcaata ggtcgaaata
     1141 ggcgagaaaa gcgcctgtcg aatcgtaaag agcaatcggc ggggggatcg tgcgaaagcg
     1201 actcgtgggt gatatagccg attgcccgtc ggattggtgg gcttcaaccg cccagtggta
     1261 cgctgtatca cggtctaatc cggaaatctc ccattcacgt gtttcagggt cttcaatcgc
     1321 gatcggcggg tcgaacggac caccttcttt cgcaaaatac agagaaaatc ccgtcaacgt
     1381 cgggggttct ctggcggaga ggttcgatcg ccctccgggc gtcgcttccc aactcaacct
     1441 caccttagtg gccacgtctg tctccgcgtt tagagggaac attaaccgaa tctccggcgg
     1501 gtagaagctt tctccttggg tcataaagca gcgttccaca ctttctaccc gttgtccgtc
     1561 agactgatgt gcgatgacct tccaacgata ctcctgatgg taattcaagg agtgtaggga
     1621 atggctattt ttcgtgagcg cgatcggaag gggtaagctc tctccggtct cgtagaagta
     1681 tagctcgtac tcggtgatga aggcgactcg ggcagaatgg ttcgtttgat ggccgggggt
     1741 ggcttgccag ctcagtgtta tcgctgtcgc gcaatccgtt tggccgtttt cggggtagag
     1801 aagggccaat atcgggggcg cataggtcgc tgctttcgtg cgaaagctcc gttcttcgct
     1861 ttccgcggat tgcccgtcgg gttgataggt gacgaccttc catcggtacg tcgtaccgta
     1921 taggagagaa gaaaccgttt tttcgcattt tcgggtatgg taaggaatac cgtacggctg
     1981 accgtcgggc cgaaggaaga gatcatactc gaggggtgaa tcggcgcgcg cttctatatt
     2041 gcatagatcc ccgtgttttc cgtcccaaac gaacgtgacc tgggtatcca tattggtttt
     2101 accatcttcc gggcctagca acctgaggga aggctcggca tatttcctgg gggcggtttg
     2161 gaacttccat tccgtgctcg tggcggtttt tccgtccgat tgcttcgcga caacatacca
     2221 tcgataggag gtgccgtaat tcagaccgct gataaggaag cagggaagat ttaccgtcgc
     2281 gaactcggtg ggttcttctg cgccatcttc ggttatatgg acgtggaact ccgaaatctc
     2341 aacaagtcga ggaaatgtct ccaacaggtc tcccggagcg gcggcccagg ttaaaatgag
     2401 ttctgtcgct tgatccgacg ctccattttc tgggaagatc aaagagattt gcggaatacc
     2461 gtattctctc ttttttgttt taaacgtcca ctcaggacta tccgctgatt ggccatcact
     2521 ttgataggcc ctcacgatcc aacggtaaag gctatgctcc gctaagtccg tgatctcgca
     2581 ctcaggttcc tgaagcagga acgtgtgctc ggcggcgcct tcgttatcta agagaaggcg
     2641 caggtcatag tgcgtgatcg tcgcggaagg aataggggtt cccgggggat ttcccggaac
     2701 ggccgaccat cgcagcacaa ggttgaaagg ctggtcgagg cttccgtcct cagggaacgg
     2761 attctctatt cgagggggct cccaagtcat aggcaccggg gtatttaagc aagaagtcac
     2821 aagcatcgcg aagccaacga taaagagtat agccacagaa atcgcgagtg agagcgcgat
     2881 gttctttacc ataaagcctc cagctaccaa acggaaatag agaacatgga cattttatca
     2941 taggttccga acctaagacc agtaacgttt ctcttttgac atctcccgcc cgccgaccat
     3001 cccctcagcg tgcccccgat tcctttgttt cggttt
//
LOCUS       DAOPXN010000227         3030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33428_length_3030_cov_3.241345, whole genome shotgun sequence.
ACCESSION   DAOPXN010000227 DAOPXN010000000
VERSION     DAOPXN010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33428_length_3030_cov_3.241345"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="PKM49_09520"
     CDS             <1..1173
                     /locus_tag="PKM49_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83071.1"
                     /translation="LTWHTSSGTPPIYYDVFLDTNAVPTTKIANGLTQTSYAPGPLSP
                     STVYYWRVEAYNAYGGPVGGLTTSFTTQASETDPSSPINLYPAHNAEGIPLQLYLVWQ
                     HSSGTQPITYDVYFGTTPTPTEVIGQNVTQNFLSTPALTANTTYYWYVVSTNALGSST
                     GTTTAFTTAGGSGLPPTNLTLLSPANGQSAVPLSTTLMWSCQGTLPITYHVWVGETPA
                     LSGSTDYKGATTTPSYALSGLTQGRTYYWKVSATNAYGAGVTSSVYSFTTATSVPVGP
                     IYLEKLPSGNGFQVKSMSYFTPVTPFVMVLDSSTINFSNMAMLPDIARKETFGNFSVI
                     LSTDANEIATSLSGFSPSNLTLFTIITTAIGTIQLNNFTSGVDVPIDPSRQSVTLP"
     gene            2154..2852
                     /locus_tag="PKM49_09525"
     CDS             2154..2852
                     /locus_tag="PKM49_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83072.1"
                     /translation="MRNWIILFVFSLLLLTACMFFEQKHSVTLAITDIYGMPVSYART
                     TWVSSNGLEFHFQADARGVAYASLPAGRYQVKVEDGFFYHIDEIVVSKPANLATSLSS
                     YDKNHIACVTRWKDGDAVDMLVAGSGVCFALMEVSGCGFISTEFPLFVLPQIGEDVET
                     RATFWVKSPDSDLSPLVYHELPRGASARILEIYPQEVLSRTVTAEDWAEGPIRTVFPV
                     TSLRNAGDLCQALL"
     gene            2849..>3030
                     /locus_tag="PKM49_09530"
     CDS             2849..>3030
                     /locus_tag="PKM49_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83073.1"
                     /translation="MISGQDGIVNVWDLIYLLNRYKTNDPSADIGRMGDTLTLPPTGP
                     FVANVNPDGYVDVWDL"
BASE COUNT          725 a          815 c          778 g          712 t
ORIGIN      
        1 ctaacctggc acaccagttc cggcacaccg cctatctatt acgacgtctt tctcgacaca
       61 aacgcagtcc cgacgacgaa aatcgcgaac ggattgacgc aaacgtccta cgcgccgggc
      121 ccactttcac cttcaacggt ttattactgg cgcgtggaag catacaacgc ctacggggga
      181 cctgtcggag gtcttacgac gagttttacc acacaagcgt cagaaacgga tccgagctct
      241 ccgataaatc tgtacccggc acataatgct gagggcatcc ccttgcaatt gtacttggta
      301 tggcaacaca gtagcggaac ccaaccgatt acctacgacg tgtatttcgg aacgacgccc
      361 acgccgacgg aagtgatagg ccagaacgtc acgcagaact tcttatcgac gccggcgctc
      421 acggcgaata caacgtatta ttggtatgtc gtatccacca acgcccttgg ctcttcgacg
      481 gggacgacga cggccttcac cacagccgga ggaagcgggt tgccacctac gaatttgact
      541 ttactgtctc ccgccaacgg gcagagcgcg gtacccctga gcacgacgtt gatgtggtct
      601 tgccagggaa cgttgccaat cacataccac gtatgggttg gtgaaacacc cgctttgtcg
      661 ggatctacag attacaaagg cgcgacaacg accccgagtt acgcgctcag cggattgacg
      721 caagggcgga cctactattg gaaagtatcc gcgacgaacg cctacggagc gggcgtgacg
      781 agctcggtat atagttttac aaccgccacc agcgtccccg taggtccgat ctatctagaa
      841 aaactgcctt ccgggaacgg attccaagta aaatcgatga gctacttcac tccagtaacg
      901 ccgtttgtga tggtactcga ttcatcgacg ataaacttct cgaatatggc gatgctcccc
      961 gatattgcgc ggaaagagac gttcggcaat ttctcggtga tactctctac cgatgcgaac
     1021 gaaatcgcta cgagcttgtc cggattctcc ccaagcaatc tgacgctttt taccatcatc
     1081 acgacggcaa tagggacgat tcaattgaac aatttcactt ccggcgtcga tgtccccatc
     1141 gatccctcaa ggcagagcgt cacattgcct taaggccata aaccaatagc gttcgggacc
     1201 gcccggaagc gtccgggatc accgagcatt atagattatc gcggggtaac caacgggacc
     1261 ccgcgattcg ctttgtgagc gcttggtggc gcagactccg tggggtaatc gcggggtaac
     1321 cagcgggctg acttcggaag cccacgaagt ctcgcgatcc cccttatgtt tttttacatc
     1381 gcgatgtagt cggggtatcg cgccgtcgca aaaagaaggg acgcttacgt cgcgatgcct
     1441 gtatgaggtt atcgcagggt aaccgaaggg accccgcgat cccttcaagc aactcagtat
     1501 ccgtcttttg tctttctcag aatcgtatcg cgccgtcgca aaaagaagaa gcgcttacgc
     1561 cgcgatgctg cgcctgggag cggtctcaga aaaacaaaag ataaaagaca tgccaaactg
     1621 aagtttggcg ttcccaggcg ctctcagata tgttctaatc tctcctctgt gttctccgtg
     1681 ttctctgtgc gaaccctgtt tttcaggact tgtacaaaag cgtatcgcgc cgtcgcgaaa
     1741 agaagaagag cttaagccgc gatgccaaca aggatcatcg cggggtaacc gaagggaccc
     1801 cgcgatccct tcaagcaact cagtatccgt cttttgtctt tctcagaatc gtatcgcgcc
     1861 gtcgcaaaaa gaagaagcgc ttacgccgcg atgctgcgct tgagagcggt cccaaaaaaa
     1921 caaaagaata aaagacatgc caaagtgaag tttggcgttc ccagagcgtt cccagctgtt
     1981 ctcaaggcgc accccgaaag gcggggcgcg gaaaacgttc tgactgccag cgcttcccgt
     2041 ttcatttctg aacctctggc tattctctcg catatttttt ccaaatcgcg tataattagg
     2101 atagaaaagg cactaatcgg caaaaggatc ctctaaagaa tggaggtgtc tctatgagaa
     2161 attggatcat tctgtttgtt ttttcgcttc tcttgctcac ggcgtgtatg ttttttgagc
     2221 aaaagcattc cgtgacactc gcgataacgg atatctatgg catgccggta tcgtacgcgc
     2281 gtacgacttg ggtgtcctca aacggtttag aattccactt tcaagccgac gcgcggggtg
     2341 tagcgtacgc gtcgttgccc gcaggccgct accaggtgaa agtcgaagac ggcttctttt
     2401 accatatcga tgaaatcgtc gtttcaaaac ctgcgaatct tgcgacgagc ctctcttcgt
     2461 atgataaaaa ccatatcgct tgcgttacga ggtggaaaga tggcgacgcg gttgatatgc
     2521 tggtcgccgg ttcgggagtt tgttttgctc taatggaggt cagcggttgt gggttcattt
     2581 cgacagagtt tccgcttttc gttttacctc aaataggcga agacgtagaa acacgggcca
     2641 cgttttgggt gaagtcgccc gactcggatc tttcgcccct cgtttatcac gaattgccaa
     2701 ggggagcgtc cgcgaggatt ctcgaaattt atccgcaaga agtcctctca agaaccgtca
     2761 cagccgagga ttgggcggaa gggccaatac ggaccgtttt ccctgttact tcgctgagaa
     2821 acgccggtga cctctgtcag gctcttcttt gatttcgggg caagacggaa tcgtcaatgt
     2881 atgggatttg atctatctac tgaatcgtta caagacgaac gacccttccg ccgatatcgg
     2941 ccgtatggga gacacgctga ctttgcctcc taccgggcct ttcgtggcga acgtaaatcc
     3001 ggatggttac gtagacgttt gggatttaat
//
LOCUS       DAOPXN010000228         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33534_length_3024_cov_4.967329, whole genome shotgun sequence.
ACCESSION   DAOPXN010000228 DAOPXN010000000
VERSION     DAOPXN010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33534_length_3024_cov_4.967329"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            827..2074
                     /locus_tag="PKM49_09535"
     CDS             827..2074
                     /locus_tag="PKM49_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNY83074.1"
                     /translation="MDRLLAELNETTIILLSLSIMLLAGFLLTRITKLAKLPNVTGYI
                     LAGVLIGPYVLNVIPSEMVANMGFISDIALAFIAFGVGRFFKKEAFQETGFGVIVITL
                     MESLMAGALVTLLMRYLFNLNWSFCLLLGAIATATAPASTMVTIRQYHARGTFVNTLL
                     QVVAFDDAVCLIAFSIASAYVNAHAGGSISASEIILPLVYNIVVLAIGILNGAILSKL
                     MTPKRSEDNRLILTISLLLGLAGLCAALDISPLLSCMVFGTTYINMTKDKELYKQVEK
                     FTPPILSIFFVVSGMNLDIGSFGTLGIIGVAYFFIRIIGKYFGAYFGCMMAGTTKAVR
                     NYLGLALVPQAGVAIGLAFLGRRLLHESSGNMLLTIILSSSVLYELIGPASAKFALFR
                     SGAIKQESLATLPQAVESSADRA"
     gene            complement(2302..2577)
                     /locus_tag="PKM49_09540"
     CDS             complement(2302..2577)
                     /locus_tag="PKM49_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNY83075.1"
                     /translation="MTKKELIGLIAESTEATQKSVDVFLNAFIEIVGEELRAGNEVRL
                     VGFGTFKVIHKKARKGINPKTLKAIDIPATKTPKFVAGKTLKDRIEK"
     gene            complement(2641..>3024)
                     /locus_tag="PKM49_09545"
     CDS             complement(2641..>3024)
                     /locus_tag="PKM49_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNY83076.1"
                     /translation="EEIKKSGVPVILGPYMCSRSKVELKDMTYKTYKGALEDGTLFAC
                     ISDHPVFPSQVLRLQAGMAVAYGADEAGMFRALTIMPAQILGIEKDFGSIEKGKQSSL
                     CIWDGHPFDSRSRVIWNDQDGWTTL"
BASE COUNT          671 a          769 c          747 g          837 t
ORIGIN      
        1 gtaactgttg tgttcgacca ccgccagtaa ggaagaaagc ttcacgtcaa acaccccgat
       61 cacctcctat tataagcaaa ccttattata caataaaatc ttataatttt actttatggg
      121 atttttgtat ataataccag ctataggccc ttcagaaaac atacgcgtta aaaaagggct
      181 cgacggagcg agcggctttt accttgagaa cgtctagcgc gtagggggtc tgcctttaag
      241 acgacaaacc gtgaatagaa aacggtacca aacaaaggct ctgcactcac cttgtcacct
      301 taagatatgg tctccggcgg atcggcatac cggttggcgt ttcgcttagg aagaaaacaa
      361 gttaggaaga aggcaagggc gatgaaaagg cgccttcgtt tttgacttga cacaaccttt
      421 catccgatcg aggggttatt ttagccggca ttttcgaggg ccacagcgca tacggttcta
      481 tatgtagaga cgcgctacaa agtgatcgcc agattcggcc gagcgaagtc gggggcgttg
      541 ggcattctcc tggccaacct cggactgacg tttttttgct ttccactatg tttttcagca
      601 gatttggtaa tatataccat aaggccgttg ttgctaataa tgggggtgaa cgtggtcccg
      661 gccccgtaga tgttggagga attcgatatg ttctggttac gtaacgggcc gaattatctt
      721 aaactcccta cccttattta ttgtctggta gcatcatcct aacggacgtt gacctgcgtt
      781 attttgccgt gtttgtaaat ataacggtgt agaggggcgt taagcgatgg atagactatt
      841 ggctgaattg aatgaaacga cgattatcct gctttctttg tccatcatgt tactggcagg
      901 atttctgctc acacggatca cgaagttggc gaaattgcct aacgtaaccg gctatatact
      961 ggcgggtgtc ctgatcgggc cttacgttct gaacgtgatt ccgagcgaga tggtggccaa
     1021 tatggggttc atcagcgaca tcgcgttggc ctttattgcc tttggcgtcg gccgtttttt
     1081 caaaaaagaa gcgtttcaag agaccggctt cggggttatc gtgattactt taatggagtc
     1141 tttgatggcc ggcgcgttag ttacgctcct gatgcgttac ctattcaacc tcaactggag
     1201 tttttgtctt cttttaggcg cgatcgcgac cgcaaccgcg cccgctagca cgatggtcac
     1261 gatacggcaa taccacgccc gaggtacttt cgtaaatacg ctcctgcaag tcgtcgcgtt
     1321 cgacgacgcc gtctgtctca tcgcttttag tatcgcttcc gcctacgtta acgcgcacgc
     1381 cggcgggagc atctccgctt ccgaaatcat cttgcctttg gtctacaaca tagtcgttct
     1441 ggctattgga atactaaacg gagcgatttt aagcaagctt atgactccaa agcgaagcga
     1501 ggacaaccgg ctgatattga ccatatcgct tcttctcggt ttggcagggc tttgcgccgc
     1561 gctcgacatt tcccctttgc tgtcttgtat ggtgtttggc accacctaca tcaatatgac
     1621 caaagacaag gagctgtaca agcaagtcga gaaatttacc ccgcccatcc tgtcgatctt
     1681 cttcgtcgta tcggggatga acttggatat cggctccttc ggtaccttag ggatcatcgg
     1741 agtcgcctat tttttcatcc ggattattgg aaaatacttt ggggcgtatt tcggctgcat
     1801 gatggccggg acgacaaaag ccgttcgcaa ctatttgggg ctggctttgg ttcctcaagc
     1861 gggggtggcg atcggtctcg cctttttggg tagaaggctt ctgcacgaaa gctccgggaa
     1921 tatgttgctc acgatcatcc tatcttcctc ggtgttgtac gaactaatcg ggccggcctc
     1981 cgcaaagttc gcgctgttcc gttccggcgc gataaaacaa gaaagccttg cgacattgcc
     2041 acaggcggtg gagtcatcgg ctgatagagc ctgagaggat catcacgtga tatagaagcg
     2101 tattagcgcc ctgtacgggc tgtcaaggct cgttgtttct tcacgttggc cggaaaaaga
     2161 ccgctaccgg cccctgagat ctcgattctg gtgaacctac gacgaggtag cctttgcaaa
     2221 aaggctacct gccttgcgaa aaagggttac ctcccgccct ggggaggctt cccttttaac
     2281 cgaaaaggcg tcggagttcg gttacttttc gatgcgatct ttaagcgttt tccccgcgac
     2341 gaacttcgga gtcttcgtgg ccgggatgtc gatagctttc agggttttcg ggttgatccc
     2401 ttttcgggcc tttttatgta tgactttgaa ggtcccgaac cccaccaatc gcacttcatt
     2461 ccctgccctc aactcttctc ccacgatctc gatgaaggca ttcaaaaata cgtccacgct
     2521 cttttgggtc gcttcggtcg actcggcgat caaccctatc aattctttct ttgtcattgt
     2581 atcccccctt tttttcttcc ggttatttta ccatatttcg atcgcttagg ttctatgggt
     2641 tcacagagtg gtccaaccgt cctgatcgtt ccagatcaca cgagaacgcg agtcgaacgg
     2701 gtgaccgtcc cagatgcata aactggactg ttttcctttc tcgatcgagc cgaagtcttt
     2761 ctctatccca agaatctgcg cgggcataat cgtcaatgcc cggaacatcc ccgcttcgtc
     2821 cgccccataa gccacggcca tccccgcctg caatctcagc acttgagagg gaaagacggg
     2881 atgatcgctt atacaagcga agagcgtacc atcttcgagc gcccctttgt aggttttgta
     2941 cgtcatatct ttcagttcca ctttggaccg cgagcacatg tacggtccaa gaatgacggg
     3001 gaccccggat tttttgatct cttc
//
LOCUS       DAOPXN010000229         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33587_length_3021_cov_7.027647, whole genome shotgun sequence.
ACCESSION   DAOPXN010000229 DAOPXN010000000
VERSION     DAOPXN010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33587_length_3021_cov_7.027647"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKM49_09550"
     CDS             <1..218
                     /locus_tag="PKM49_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015075603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNY83077.1"
                     /translation="FVEFIALIYLSYVKKRMQDAKLFERWTLQGLLDELDSIELYEVP
                     GHGRILSEVTKKQEQLYRDLGVNPPSL"
     gene            complement(361..1143)
                     /locus_tag="PKM49_09555"
     CDS             complement(361..1143)
                     /locus_tag="PKM49_09555"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HNY83078.1"
                     /translation="MVGRLEEGEHLKSLRLRGPGIEQEIPAFVFGEEVCAQHPLSLNA
                     PDYTDFSLIFLLDDGAFTTPLVCFGNVLFAGDAPALELDIEITGRHVVQAGEYLYRIA
                     QLHQSSVAFLEILNQTSALIYPGQILRVGRVSVSGSPIVLSISLSDCQLTFFLEGTPL
                     RTYPVAVGRGQSSRPGTYFITRKIPNPALYWEGEYIQPLAPINGLGTWWLELSNPQYG
                     IHGTNRPWEIGKRVSHGCIRLFNEDIEQLQRLLPIGTIVKIY"
     gene            complement(1290..1523)
                     /locus_tag="PKM49_09560"
     CDS             complement(1290..1523)
                     /locus_tag="PKM49_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNY83079.1"
                     /translation="MYKLQYHYDEMLKKVPYKYAIPILVGKRAERLKEERALRGEHSE
                     LSKLMEIAMEEVYAKKVKIRNEDMMKLLKPNVK"
     gene            complement(1527..2165)
                     /gene="gmk"
                     /locus_tag="PKM49_09565"
     CDS             complement(1527..2165)
                     /gene="gmk"
                     /locus_tag="PKM49_09565"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNY83080.1"
                     /translation="MRKGLLFVVSGPSGAGKTTVMEKVLELQGEKLIFSISYTTRKPR
                     ITEVDGVQYHFIDRLHFDRLIEQGDFLEYAQVHGHYYGTSRSFVYPLLEKGQDVLLDI
                     DVQGALNVKKALPEAILIFLAPPSLRELATRLRKRATEKKEEVYQRLADSKWEMSHIR
                     DFQYLIVNRSVERSVRELEAIIEAYRCLVVSRAEELRAFEEEMKDFKIESEE"
     gene            complement(2171..2452)
                     /locus_tag="PKM49_09570"
     CDS             complement(2171..2452)
                     /locus_tag="PKM49_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNY83081.1"
                     /translation="MYGIINIGFGNIIVGDRVIAIVNPESAPMKRLKEQAKDDGKLID
                     ATYGRKTRAIVITDSNHVILSAIQPETIALRFRQSFEELEKVLQNIRVV"
     gene            complement(2678..>3021)
                     /locus_tag="PKM49_09575"
     CDS             complement(2678..>3021)
                     /locus_tag="PKM49_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY83082.1"
                     /translation="QLSELMKTVSEERSEKMQTIADMLRQEGMEKGIIKGREEGREEG
                     REEGLEKGIIKGKEEGREELLWKLIAKKFPKASQKYFERLKSLTIEQLDSLGLELIDM
                     KNEEELKKHLM"
BASE COUNT          715 a          791 c          700 g          815 t
ORIGIN      
        1 tatttgtgga atttatcgca ttaatatacc tgtcctatgt gaagaagcga atgcaagacg
       61 cgaagttgtt cgagagatgg acgcttcagg gcttgttgga tgaattggac tctatcgagc
      121 tgtatgaggt tcctggccac ggaagaatcc tcagcgaggt caccaaaaag caggaacaac
      181 tctatcggga tttgggtgtc aatccgccat cgttataaat tccgggaatg tggttattaa
      241 ggcaaagggt cagaaaatgt aggcaccttt cattaatgaa tgtgttcgta gagacttcga
      301 agagagtcct ttcataagag ccaagttaag aacccccctt cgtttttctt ccgaaaagcc
      361 ctaatatatc ttgacgatag ttccgatggg gagcaggcgt tgcagttgtt cgatgtcttc
      421 gttgaacaac cgtatgcagc cgtgggagac gcgcttgccg atctcccaag gtcggttggt
      481 accgtggata ccatactgcg ggttgcttag ctccagccac caggtgccta atccgttgat
      541 tggggctagg ggttgaatgt actctccttc ccaatagaga gcagggttag gtatttttcg
      601 agtgataaaa taagtccccg gtctcgaact ctgccctctc ccgacagcga ccgggtaggt
      661 tctgagtggt gtcccttcca aaaaaaacgt gagctgacag tcggataggc tgatagacag
      721 aacgatcggg gatcctgaca cgctgaccct tccgacacga agaatttgcc caggatagat
      781 aagcgcgctg gtttgattca gtatttccag aaacgctacg gagctttggt gcaactgagc
      841 gatgcggtac aggtattctc ccgcttgaac gacgtgccga ccggtgatct ctatgtcgag
      901 ctccaaagcc ggcgcgtcac cggcgaagag gacatttccg aaacagacca aaggggtggt
      961 gaacgcgccg tcgtcgagta ggaagatcaa cgaaaaatcg gtataatcgg gcgcattcaa
     1021 ggagagcgga tgttgggcgc agacctcttc tccaaaaaca aaagcaggaa tctcttgttc
     1081 gattccgggg cctctcaaac gcaggctctt taaatgctcc ccctcctcga gccgccctac
     1141 cacctgaaaa agaaaggccc cctcaggcat ctccgcttcg gtgccaggga gcgcggatga
     1201 aaataccgta atgcttctgg ctgctaaacc gccgacgaac cacaaaacaa gcgccagaaa
     1261 aatgagtcgc gcgccttttc ttttcaatct tattttacgt tgggctttaa aagtttcatc
     1321 atatcttcgt tccggatctt caccttttta gcgtagactt cttccatcgc aatctccatc
     1381 agcttcgaca gttccgagtg ctctccgcgc aacgcgcgct cttctttcaa acgttcggcc
     1441 cgcttcccca ccaagatggg aatggcgtac ttgtaaggca cctttttaag catctcatcg
     1501 taatgatact gcagtttgta cacgacttac tcctcgcttt ctattttaaa atctttcatc
     1561 tcttcctcaa aagcgcgtag ttcctccgcg cgagaaacca cgaggcatcg ataggcttcg
     1621 attatggctt ccagctcccg gacggaccgc tcgacggagc gattcacgat gagatactga
     1681 aaatcgcgga tatgggacat ctcccatttc gaatcggcca atcgttggta tacctcttct
     1741 ttcttttccg tggcgcgttt gcgtaaacgg gtcgcgagct ctctcaagct cggaggcgcc
     1801 aaaaagatca gaatcgcctc gggcagggct ttcttgacgt tcaatgctcc ctgaacgtcg
     1861 atatccagca aaacgtcttg ccccttctcg agcagagggt agacgaaact gcgggaggtg
     1921 ccgtagtagt ggccgtggac ttgcgcgtac tccaaaaaat ctccctgttc tatgagccga
     1981 tcgaagtgta gtcggtcgat gaagtggtat tgaacgccgt cgacctccgt tatcctcggc
     2041 tttcgggtcg tgtaggagat agaaaagatc agtttctccc cttgaagctc taagaccttt
     2101 tccataaccg tcgtttttcc ggcgcctgaa ggaccggaaa cgacaaacag gagccctttt
     2161 cgcataccgt tcaaacgacc cggatattct gcagcacttt ttccagctct tcgaagctct
     2221 gcctgaatcg caacgcgatg gtttcgggtt gtatcgcgct taaaatgacg tggttgctat
     2281 cggtgatcac gatagcccgc gtcttcctcc cataggtggc gtcgatcaac tttccatcat
     2341 ctttggcctg ctcttttaac cgtttcatcg gagcggattc agggttgacg atcgcaatca
     2401 cccgatcccc aacgatgata ttcccaaacc cgatgttgat gattccgtac attcaggcac
     2461 ctccaggcgt ccccagccac agcatacccg taccgataat tacctctctg attatagcat
     2521 aacggtagaa atttcctctg gcacgtgtgg gtatcatacc ggcgtgtgat aagaaaaaaa
     2581 gacaaaaacg aggagggaaa aaaaagctcc cgaaggagcc accataacat cgcacacttg
     2641 acccatcaaa tcgcgttcaa gccttttgtc ggactctcta cattaaatgc ttctttaact
     2701 cttcctcgtt tttcatatcg atcaactcaa gtcccaagga gtcaagctgc tctattgtaa
     2761 gggacttcag tctttcgaaa tatttttgcg aggctttagg aaattttttg gctattaatt
     2821 tccaaaggag ttcttcccgg ccttcttctt tgcctttaat aatccccttt tctaagcctt
     2881 cttctctgcc ttcttctctg ccttcttctc tgcctttaat aattcccttt tccatgcctt
     2941 cttgtcttag catatccgcg attgtttgca tcttttcgct cctttcttcc gataccgttt
     3001 tcatcagttc tgacaattgt t
//
LOCUS       DAOPXN010000230         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_33702_length_3014_cov_5.482258, whole genome shotgun sequence.
ACCESSION   DAOPXN010000230 DAOPXN010000000
VERSION     DAOPXN010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33702_length_3014_cov_5.482258"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2249
                     /gene="mutS"
                     /locus_tag="PKM49_09580"
     CDS             <1..2249
                     /gene="mutS"
                     /locus_tag="PKM49_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNY83083.1"
                     /translation="DPALAKGIVRREVTRVVTPGTVIEENLVDMNSSNFLAAVWEDKD
                     FFACLLDISTGESYVFVRNSWEQIVDLFQKRGITQLLVPEQQEWDLRSAPLKKRMPAL
                     FVEKLPDWYFHKEEAREQIKRFFKVSYLDYLELPTSAQTLYGAVLKYLENQYMTAVDH
                     FRPPILVRDHTTLWMDFATIDHLNLIHRESGSAGTSLYDILNETATPMGSRLLKQWIL
                     FPLREVSEIEKRLDRIDALLAKPAKMESLKEILAGYFDLERIASRISYGKVNPKDLAG
                     LRQSLFDITLLNEILTEYKDPKQVLPRPEGLPDLQNLLETAIKETPSRAVGEGEVIGE
                     GFDPELDEIRELYLHSDNALRALEFQERERTGIGSLKVSYNSVFGYYIEVPKSQTSRV
                     PPDYERRQTLVASERYMSPALKPLEEKLLNARERLAFLEKRAYLRLIELLKPFIPKIQ
                     AISERVAVLDVACALAFCALKRRFKRPSFSPDRILEIHQGRHPVIEQNQDFFIPNDLR
                     LDKDHRFVILTGPNMAGKSTFLRQAAVIVVMAQMGCFVPVESAKVPIFDRIFTRIGAR
                     DDLSSGKSTFLVEMTETAAILNNATDRSLIILDEIGRGTSTYDGISIAWVVSEYILNA
                     IGAYCVFATHYNELTELGSLYPGIHNFRIKVTEENGEVLFLYKVEEGAADRSYGIEVA
                     RIAGLPPEVIARANQVLETITAENRLESKVRVLGSSELEKMKQKLSRSKRVIRNQMSF
                     FDKEDFST"
     gene            2246..>3014
                     /locus_tag="PKM49_09585"
     CDS             2246..>3014
                     /locus_tag="PKM49_09585"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HNY83084.1"
                     /translation="MIVTPSQMRTFEQNAIAREGIASLSLMENAGLAVAHHVLELARK
                     APGLSTKPVLILCGKGNNAGDGMVVARELHRRQWPTHTVFCLGRSFTGDAKTEYDRLM
                     ASEAPTFDATDSAWLRCDYGMVLDAIFGTGANKPLPEEICRLLLRVNALPGLKVALDV
                     PTGIHPETGSLLAQHPFEADETLTFHCAKTGLYLSPGRFFSGKVTVLPIGIAHSEEGV
                     SRTLADEELIRRIFPKRVAWGHKGTFGKVGVLGGSIRY"
BASE COUNT          679 a          847 c          875 g          613 t
ORIGIN      
        1 aggatccggc cctcgccaaa gggatcgtgc ggcgagaggt cacgcgcgtg gtgacaccgg
       61 gcacggtgat cgaagagaac ctcgtcgata tgaatagctc gaacttcttg gcggcggttt
      121 gggaagacaa agactttttt gcctgcttgc tggacatctc caccggtgag tcctacgtct
      181 tcgtccgcaa tagctgggaa cagatcgtcg accttttcca aaaacgtggc atcacgcagt
      241 tgctcgttcc cgaacagcag gaatgggacc ttcggtcggc acccctcaaa aagcggatgc
      301 ccgccctctt cgtcgaaaaa ctcccggatt ggtatttcca taaagaagaa gcgcgagagc
      361 agatcaaacg gtttttcaag gtctcctatt tggactattt ggagctgccc acctcggcgc
      421 aaacgcttta cggcgctgtc ctgaagtacc tagaaaacca atatatgacc gcggtggacc
      481 attttcgccc ccccatcctc gttcgcgatc acaccacact gtggatggat ttcgcgacga
      541 tcgaccacct gaacctgatc caccgggaaa gcggttcggc gggtacctcg ctgtacgata
      601 tcttgaacga aaccgccacg ccaatgggaa gccgcctcct caaacagtgg atcctgtttc
      661 cgttgagaga ggtctcggag atcgagaaaa ggctggaccg gatcgacgcc ctcttggcaa
      721 aaccggccaa aatggaaagt ctgaaggaga ttctagccgg atacttcgac ctggagcgca
      781 tcgcctcacg tatctcttat gggaaggtga accccaaaga cttggccgga ctacggcaat
      841 cgttgttcga tattaccttg ctgaacgaga ttttgacaga gtacaaggac ccgaaacagg
      901 tcctaccgcg tcccgaaggc ctgcccgatc ttcaaaacct tttggaaacg gccattaagg
      961 agacccctag tcgggcggtc ggcgaagggg aagtcatcgg ggaagggttc gacccggagc
     1021 tcgatgagat tcgcgaattg tacttgcaca gcgacaacgc gttgagagcg ttggaatttc
     1081 aagagcgaga gcggacaggg atcggttctc tgaaggtctc gtacaattcg gtcttcggat
     1141 actatatcga ggtcccgaaa tcccaaacct cacgggtccc gccggactat gaacgccgcc
     1201 aaacgctggt agccagcgag cggtatatga gcccggcttt gaaacccctc gaggagaaat
     1261 tgctcaacgc gcgagaacgc ctggcctttc tggaaaaacg ggcctattta cggttgatag
     1321 aactgctgaa acccttcatt ccgaaaatac aagcgatatc cgaacgcgtc gcggtgttgg
     1381 atgtcgcgtg cgcgctcgcc ttttgcgcac tgaaacggcg gttcaaacgg ccttcctttt
     1441 cgcccgatcg gatccttgag attcatcaag ggcgtcaccc ggtgatcgag cagaaccagg
     1501 atttttttat cccgaacgat ctgcggttgg ataaggacca tcggttcgtg atcctgaccg
     1561 ggccgaatat ggcggggaaa tcgacctttc tgcgtcaggc ggccgtgatc gtcgtgatgg
     1621 cccaaatggg ctgcttcgtg ccggtcgagt cagccaaggt gccgatcttc gaccggatct
     1681 tcacccgaat cggcgctcgg gacgacctga gctcgggcaa gagcaccttc ttggtcgaaa
     1741 tgacggagac cgccgcgatt ctgaacaacg ccaccgatcg atccctgatc attttggacg
     1801 agatcggacg cgggaccagc acctacgacg gcatcagcat cgcctgggtc gtgtcggagt
     1861 acatcttgaa cgcgatcggc gcgtattgcg tcttcgccac ccattacaac gagctgacgg
     1921 aactgggttc gctttaccca ggtatacaca acttccggat caaagtgacc gaggaaaacg
     1981 gagaagtgct cttcctctac aaggtggagg aaggcgccgc ggatcgcagc tatgggatcg
     2041 aggtcgcgcg tatcgcggga ttgccgcccg aagtgatcgc gcgcgctaat caggtgttag
     2101 agacgatcac cgccgagaac cgcttggaat ctaaagtgcg tgttttggga agctcggagc
     2161 tggaaaaaat gaagcaaaag ctctcgcgga gcaagcgggt tatccgaaac caaatgtcct
     2221 ttttcgataa ggaggatttt tcgacatgat cgtaaccccg tcccagatgc ggacttttga
     2281 gcagaacgcc atcgcccgag agggaatcgc ttccctgtcg ctgatggaaa acgccggact
     2341 cgccgtggcg caccacgtct tggagctggc gagaaaagcg cccgggctct cgacaaaacc
     2401 ggttttgatc ctgtgcggaa aagggaacaa cgccggtgac gggatggtcg tcgcccgcga
     2461 gctgcaccgg cgccagtggc ccacgcatac ggtcttctgc ctgggccgga gcttcaccgg
     2521 ggacgcgaaa accgagtacg atcgattgat ggcgtccgaa gcgccaacct tcgacgccac
     2581 ggattcggct tggctgcgat gtgattacgg tatggtgttg gacgctattt tcggaaccgg
     2641 cgcgaacaag cccttacccg aagaaatctg ccggttgctt ctcagggtca acgcgcttcc
     2701 gggcctaaag gtcgcgctcg atgtgccgac cggcatccac ccggaaaccg gaagcctctt
     2761 ggcccagcac cccttcgagg cagacgaaac cttgactttc cattgcgcga agaccggact
     2821 ctacctttcc cccgggcgct tcttcagcgg gaaggtgacc gtcttgccga tcggtatcgc
     2881 gcacagcgaa gagggcgtct cgcgcactct cgccgacgag gagctcattc gacggatttt
     2941 cccgaaacgc gtcgcttggg ggcataaagg gaccttcggg aaggtcggcg ttttgggggg
     3001 gagcatccgg tatc
//
LOCUS       DAOPXN010000231         2986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_34158_length_2986_cov_4.125213, whole genome shotgun sequence.
ACCESSION   DAOPXN010000231 DAOPXN010000000
VERSION     DAOPXN010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34158_length_2986_cov_4.125213"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKM49_09590"
     CDS             complement(<1..403)
                     /locus_tag="PKM49_09590"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNY83085.1"
                     /translation="MNSTKSFTLQTQAGSEKIGEALSAGRNGFLRLPHGDVETPVFMP
                     VGTQAAVKCLLPGQLKEIGTQILLSNTFHLYLRPGHEDIRFHGGLHRFMGWDRPILTD
                     SGGFQVFSLSKLNKITDEGVVFRSPLDGSEHF"
     gene            complement(400..1011)
                     /locus_tag="PKM49_09595"
     CDS             complement(400..1011)
                     /locus_tag="PKM49_09595"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HNY83086.1"
                     /translation="MDFRKMSIDEFLDTLGSSSPTPGGGTVSALSGALAAGLTKMVAS
                     LTQGKKGYEDHFPLMQETALLETQEIEWFKTMMDEDSKAFNQVTRAMKLPKDTPTQKE
                     VRAEAIQAALKAATKTPLEIAKHITRLVPRAGLMARFGNKNAKSDALCALELCQAGFR
                     MSLENVSINLEMIKDSQFVQKTREAVSELKTQFEEGMKQALEA"
     gene            1383..>2986
                     /locus_tag="PKM49_09600"
     CDS             1383..>2986
                     /locus_tag="PKM49_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83087.1"
                     /translation="MDRNNFKYPLKAYVDGAYRDGGVGYGFIIFDRSTVLAERSEPVP
                     KGIWADSRQVGGELLATMAVLAWCDDRGVREITVFYDYEGIRAWATGKWRAKKELTQT
                     YVKEIQSRKVKVRWVKVKSHSGDPYNERVDRLAKEGAAGNSYYWEEKMQLKEAVDNAV
                     LSKMKEARNLYKEKAYAQALPLFENLWLYNRPLFYPDDMYSFCVCLRKDRRSPDALEV
                     AEALIAAEPNNTRFNNIYGWILYDCLFHDKSRVWGAELLEQAQKILSKTKQEPNSPYE
                     LTVIKTLEWLYGLLDLPEGHLIESWIEKVNPETLSVLAYNFEEGRGKTVRKPSNAEIW
                     YLAKAKWLLSESRFSDCFLCCNEALALIAERIPDGDTAPFLRLRGAARFQLGSPKEAL
                     PDLLAAQKLEKKWPLPLDIAKVYFALGDQNNAYKYALKAALFPGSELIKLECYSLLAD
                     IYRDRSMWAMERKCLLMYQLVLVSLEIEGDPETTKRAQTIACPPGLSFEQLYKELIPI
                     WRMSLKPLLTPVQGKAVKRPIPKKGF"
BASE COUNT          763 a          726 c          770 g          727 t
ORIGIN      
        1 agaaatgttc ggacccgtcc agcggggatc tgaaaacaac gccctcatcc gtgattttgt
       61 tgagcttgga taggctgaac acttggaaac ccccgctatc cgtcagaatc gggcgatccc
      121 accccataaa ccgatgcagc ccgccatgaa accggatgtc ttcgtgtccg ggccttaaat
      181 acaagtgaaa ggtgttgctc aacaggatct gtgtcccgat ctctttcaat tgtcccggta
      241 ggagacactt gaccgcggcc tgcgtcccaa cgggcataaa gacaggtgtt tcgacgtcac
      301 cgtggggaag gcgcagaaat ccgtttctac cggccgatag ggcctctccg atcttttcgc
      361 tccccgcttg ggtttgaaga gtaaagcttt tggtagagtt catgcctcga gcgcttgctt
      421 cattccctct tcgaattgag ttttcaattc cgagacggct tctctggtct tctggacaaa
      481 ctgcgaatcc ttgatcatct cgaggttgat cgacacgttc tccaaggaca tcctaaaacc
      541 ggcctggcac agctcgagcg cgcataaggc gtcggatttc gcgtttttgt tgccgaaacg
      601 ggccatcaag ccggctcgtg ggactaaccg cgtgatatgt ttggcaattt ccaggggagt
      661 tttcgtcgcc gccttgagag ccgcttgtat cgcctccgcc cgaacctctt tctgggtagg
      721 tgtgtctttc ggaagcttca tcgccctcgt cacctgattg aaggccttag aatcctcatc
      781 catcatcgtc ttgaaccact ctatttcttg agtttccaaa agggccgttt cttgcatcaa
      841 aggaaagtgg tcttcatacc cttttttccc ttgtgtcaag gaagcgacca tttttgtaag
      901 gcccgcagcc agagcgccgg acaacgctga aacagtgccc ccaccggggg tgggtgaaga
      961 agaaccgaga gtatcgagaa actcgtctat cgacattttc cgaaaatcca tagaacccct
     1021 ccattatcat aattcatcga atgataaatt atagcacaat tactaaaaaa aagcatctgc
     1081 ctgcgttgga gaaaaaaatg gggcgcatcg gtaaaaatgg cgctcccgag caaaagactc
     1141 aagcttttcg aggaaatcct aaagaataaa gaccataaaa cctggaagtc gacgtcccta
     1201 atcgcccgtc acgaccgctc attgggcccg acttttgcct ttttgttttc tcgctttctc
     1261 tctattggcg ccttgttgtt ttccgcgcga atcgtacgcc tttcgatttc gctgtttttt
     1321 atacaaggtt tttcggtata ctataaccgc gaaggaaaac gtaaaaaagg tgggagaggt
     1381 caatggatag aaacaatttt aaatatcccc tgaaggcgta tgtcgacggc gcttaccggg
     1441 acggtggtgt cgggtatggg tttatcattt tcgatcggtc gaccgtcctt gctgaacgga
     1501 gtgaaccagt acctaaagga atttgggcag attcgcggca ggttggcggc gagttgttgg
     1561 cgacaatggc cgtgttggcc tggtgtgacg ataggggagt tcgcgagatc accgtgttct
     1621 atgactatga aggcattcga gcttgggcga caggaaaatg gagggcgaaa aaggagctta
     1681 ctcaaaccta tgtgaaagag atccaaagcc gcaaggtaaa ggtgcgatgg gtgaaggtga
     1741 aaagtcatag cggagaccca tataacgaac gggtcgaccg tttggccaag gaaggagcag
     1801 ccggaaattc ttactattgg gaagagaaaa tgcaactcaa ggaagccgtg gataacgcgg
     1861 ttctttcaaa aatgaaagag gctaggaacc tgtataagga gaaagcttat gcgcaggccc
     1921 ttcccctctt cgaaaacttg tggctataca atcgcccgct gttttacccc gatgatatgt
     1981 atagtttttg tgtttgtttg cggaaggacc gaagatcgcc cgacgcgctc gaagttgccg
     2041 aagccttaat cgcagcggaa ccgaacaaca cccgattcaa caacatctac ggatggatcc
     2101 tttacgactg tctttttcac gataaatccc gggtttgggg agcagaactt ttagagcagg
     2161 cgcaaaaaat attgtcgaaa acgaaacagg aacccaattc tccatacgaa ctcactgtga
     2221 taaaaactct agagtggttg tacggacttt tggatttacc ggaagggcat ctgatagaga
     2281 gttggataga gaaggtgaat cctgagacgt taagcgtact tgcctacaat tttgaagagg
     2341 gcagaggaaa gaccgttcgg aagccttcca acgctgaaat ctggtacctc gcgaaggcga
     2401 aatggctact gtcagaatct cgattttctg actgcttcct ctgctgcaac gaggccctag
     2461 cccttatcgc ggaaaggata ccagatggcg acaccgctcc attcctccgc ctgaggggcg
     2521 ctgcccgttt tcaacttggc agtcctaaag aggccctgcc ggacctgctg gcggcccaga
     2581 aactcgagaa gaagtggcca ctccctctgg atatcgcaaa ggtgtatttc gccctcggcg
     2641 accagaataa tgcctacaaa tacgcgctga aagccgcctt gtttccaggc agcgaactca
     2701 tcaaattaga atgttatagt cttctggccg acatctaccg agatcgaagt atgtgggcaa
     2761 tggagcgcaa gtgtctactg atgtaccagc ttgtgttggt gtctttagaa atagaaggag
     2821 atcccgaaac gactaagaga gctcagacca ttgcttgtcc gccggggtta tcttttgagc
     2881 agctctacaa agagttgatc ccaatctgga ggatgtcgct caaaccctta ctgacaccgg
     2941 ttcagggaaa agcggttaag aggccgatac cgaagaaagg gttcat
//
LOCUS       DAOPXN010000232         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_34525_length_2964_cov_5.445858, whole genome shotgun sequence.
ACCESSION   DAOPXN010000232 DAOPXN010000000
VERSION     DAOPXN010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34525_length_2964_cov_5.445858"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..1049
                     /locus_tag="PKM49_09605"
     CDS             27..1049
                     /locus_tag="PKM49_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Inner-membrane translocator"
                     /protein_id="HNY83088.1"
                     /translation="MLGIVEQGLILGLMSIGVFVSFRIVNMADLTVDGTFALGGALCV
                     RLFSLGLNAPISLLLSLLAGASAGVLTGYLHRKLRINVLLAGILVMTMLYSINLRIMD
                     GPNLPIPKSSVSSSLGTYEQVAGKDPLADLFESSAPVAKPQKETTRQRTYQNIFRGMA
                     DGQDLFLLSVIILIGVTLTGLFLRTELGTALRGFGTNPSGLESFGLPKTLMTLLGLGL
                     ANGFVSLAGGIFALYSGFADVTMGQGMIVTGLAVVMVGEILLVKKKIVYGLFAPILGG
                     IVYQGILAVVMRYGYRIGFRASDMKLLTAIFIVAVIGFGLVSTNKRMKGKFRLSTIFG
                     GGVSRD"
     gene            1042..1809
                     /locus_tag="PKM49_09610"
     CDS             1042..1809
                     /locus_tag="PKM49_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNY83089.1"
                     /translation="MIELSHVSVVYRKGEENEKCALKNATLRVAEGDFVIIVGPNGAG
                     KSTLMKVLLCELAPTIGTYTLNGRAMRGFSVTQVARWVGRVFQDPNSGIFPDLSILEN
                     LSIASKKGIRGLRFRSCPQKAVTLLSRLGLGLENTLNVRAGDLSGGQKQALAMVMAII
                     SDPKVLLLDEHTASLDPKSSVKVMELTRQINEQFGITVVMITHNMPFAEQYGNRKWRI
                     EEGEVFEYEYRPPLRRTHLETPVVLLRDQPHYEPKLV"
     gene            2135..>2964
                     /locus_tag="PKM49_09615"
     CDS             2135..>2964
                     /locus_tag="PKM49_09615"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY83090.1"
                     /translation="MKRRLWVLLLCVVVSVIGFSTVRIGITAIVDHPALDSAREGIIE
                     ALARKGMVLGTDFIVEFQSAQGNMNTAVSIANHFRSGDFAVVVGISTPSAQTLANAIK
                     NIPVVFTAVTDPVDAELIPSLGKNPGNVVGVSDMTPVKTQFQLLKLLVPTAKKVGIVT
                     NPGEANSVIITEHARQACEELGLRLVEIVGSNSSEMIGALTATVGGLDALYVGTDNTA
                     ASCMESLHRIAVSNKVPMLCGDFTLARSGGVVAYGFDYFNLGLDTGQIVWELINGKPV
                     S"
BASE COUNT          671 a          735 c          862 g          696 t
ORIGIN      
        1 actcaaataa ccgatagggg tgttttatgt tagggatcgt ggaacaaggg ttaatcttgg
       61 gattgatgtc gataggggtc tttgtgtctt ttcgtatcgt gaacatggcg gatttaacgg
      121 tggacggtac cttcgcgctc gggggcgcct tgtgcgtcag gctgttctcc ctcggactga
      181 acgcgccgat ttctctcctg ttgagtctcc tggccggcgc cagcgccggt gttcttaccg
      241 gttatctaca ccgcaaactc cgcatcaatg tcttgctcgc cggcatcttg gtgatgacga
      301 tgctctattc catcaatctt cggataatgg acggtccaaa cctcccgatc ccaaaatcgt
      361 cggtttcctc ttctttgggc acctacgagc aggtggccgg aaaggatccc ctcgccgatc
      421 tgttcgagtc ttccgccccc gtcgccaaac cccaaaaaga gacgaccagg cagagaacct
      481 accagaacat cttccgcggg atggcagacg gtcaagacct cttccttctg tcggtgatca
      541 ttctgatcgg cgtcaccttg accgggcttt ttctccgaac ggagctgggc acagcgctac
      601 gcggattcgg aacgaatccg tccggtttgg aatccttcgg gttgcccaag accttgatga
      661 ccttgttggg attggggtta gcgaatggct tcgtgagtct cgccggcgga atattcgccc
      721 tatacagcgg attcgccgat gtcacgatgg gacagggaat gatcgtcacc ggtctggcgg
      781 tcgtgatggt gggcgagatc ttactcgtca agaaaaaaat cgtctacggc ctctttgcgc
      841 cgatcttggg cgggatcgtt tatcaaggga ttctggccgt cgtgatgcgg tatggctatc
      901 gaataggatt cagagcgagt gatatgaagc tgctcaccgc gatcttcatc gtggcggtca
      961 ttggcttcgg attggtttcg acgaataaac gtatgaaagg caagtttcgt ctatctacca
     1021 tctttggcgg gggtgtgagc cgtgattgaa ctgtctcatg tcagtgtggt ttatagaaaa
     1081 ggagaagaga acgaaaaatg cgcgttgaaa aacgcgactt tgcgggttgc ggaaggggac
     1141 ttcgtaatca tcgtcggacc gaatggggcg ggaaagtcga cgctgatgaa ggtgctcttg
     1201 tgcgaactgg cgccaacgat aggaacgtac actctgaacg ggagggcgat gcgcgggttt
     1261 tcggtcacgc aagtcgcgcg ttgggtcggg cgcgtctttc aagaccccaa ctccggtatc
     1321 tttcccgacc tttccatact ggagaacttg tcaatcgcct ctaaaaaagg aatccgaggg
     1381 ctgcgatttc gaagctgccc gcaaaaagcg gtgacgttgc tttcgcgtct cggtttggga
     1441 ttggaaaaca ccttgaacgt acgggccgga gacctctccg gagggcaaaa acaagccttg
     1501 gcaatggtga tggcgatcat ctcggatccc aaagttttac tgttggacga gcataccgcc
     1561 tctctggacc caaagagctc ggtgaaggtg atggaactga cccgtcaaat caacgagcag
     1621 ttcggaatca ccgtcgtgat gatcacccac aatatgccct tcgcggagca atacgggaac
     1681 cgaaagtggc ggatcgagga aggcgaggtt ttcgagtacg aataccgccc acccctccgt
     1741 agaacccatt tggaaacccc ggttgtcctt ctgagggatc agccgcatta cgagccaaaa
     1801 ttggtttaat tgcgcgacgc tcaaaccgcc gaagaacccc aaaatccggt taacgaacgt
     1861 ctcagagggg atgtgtgacc tttggaccgg aaagccgccg aatagggccg cgcgatttgg
     1921 ccctatgaaa ggaagtatgg atagatgcga gaagatagga tgactgcaat gagtaaaatc
     1981 ttagaaacta aaaatcgaat ttttgtctac gcttattatc gggcaaagcc ctcctgtggc
     2041 tttgcttttt ttccgtgatt taggacgaaa gcgaaaaaca caagagggca tcggaaacaa
     2101 gatgccctct ttttatttta aaggaggaat caaaatgaag agaaggttgt gggtactgtt
     2161 gttgtgtgtg gttgtgagtg taatcgggtt ttctactgta aggatcggga tcaccgctat
     2221 cgtcgatcat ccggcgttgg attcggcgcg cgaggggatc atcgaggcgt tggctcgaaa
     2281 aggtatggtt cttggaacgg attttatcgt cgagttccaa tcggcgcagg gtaatatgaa
     2341 caccgccgtg tcgatcgcca atcatttccg ctccggggac ttcgccgtgg tcgtcggcat
     2401 ctcgaccccg tcggcgcaaa ccctcgccaa tgccattaaa aacatccccg tcgtgtttac
     2461 cgcggtcaca gacccggtcg acgcagaact gatcccttcc ttaggcaaga atccggggaa
     2521 cgtggtcggc gtgagcgata tgacccctgt gaagacacag tttcagctgt tgaagctcct
     2581 cgttcccacg gcgaaaaagg tggggatcgt caccaatccg ggagaagcca attcggtgat
     2641 cataaccgaa cacgcacgcc aagcttgcga agagctgggg ttgagactcg tggagatcgt
     2701 gggcagcaat tcttcggaga tgatcggcgc attgacggcc accgttgggg gattggacgc
     2761 gctctacgtc gggacggaca acacggccgc atcctgtatg gaatccttgc accggatagc
     2821 ggtgtcgaac aaagtgccta tgctgtgcgg cgacttcact ctcgcgagat ccggcggtgt
     2881 ggtagcctac gggttcgatt acttcaactt gggactcgac acaggtcaga tcgtgtggga
     2941 gctcatcaac gggaagccgg tgtc
//
LOCUS       DAOPXN010000233         2914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_35292_length_2914_cov_4.945435, whole genome shotgun sequence.
ACCESSION   DAOPXN010000233 DAOPXN010000000
VERSION     DAOPXN010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35292_length_2914_cov_4.945435"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PKM49_09620"
     CDS             complement(<1..334)
                     /locus_tag="PKM49_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83091.1"
                     /translation="MKTAMEIKMILQTYNEIATIKGTARALGIAKETVKKYLKRYWEL
                     HNGERSRISPESEERVIQVHPAGRTVEFDWGEIKLTIDGIEDTYQLGAFMDNGEMIAV
                     HRIPEEKGK"
     gene            complement(997..2241)
                     /locus_tag="PKM49_09625"
     CDS             complement(997..2241)
                     /locus_tag="PKM49_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HNY83092.1"
                     /translation="MQLFLLGLQHLFTMFGATVVVPILTGIPVSVALFTSGVGTLFFH
                     LLTKGKVPVYLGSSFAFISPIIAVTSYYAGREDALAYALGGVVVSGIVYVIFSAIIRY
                     LGPDRITKIFPPVVTGTMISLIGLILSPVAVNWASKNWVLAIVVIATIIYVKLYTRGF
                     ISMLPILIGIAVGYVVSLFFGVPEYRFDQWFSFPLFSLPKFSLYAIGIVVPAAIAPAL
                     EHIGDIYAVSMVTNKPFVKDPGLHRTILADGLATSLAALIGGPANTTYSENTGVLALT
                     RQFNPWVMRIAAILAILLSFIDPLTRFIQTAIPEPVMGGACIVLFGMIATTGLRVLVE
                     NKVAFTSKNMLIMSIMLLCGIGGANLSVGSFSLQGLGLAGVIGIVLNAILKETELKHS
                     AENTSLILTTKSKEEEYNDRSS"
     gene            2361..2726
                     /locus_tag="PKM49_09630"
     CDS             2361..2726
                     /locus_tag="PKM49_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007430035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNY83093.1"
                     /translation="MSARILIVDDSLFMRTQIRNILSGAGYEIAGEASDGNQAEEKFK
                     DLNPDLVIMDITMPGTNGIDALRKIKSLNSQARVIMCTSLGLTSKVKEAISEGADDYI
                     LKPFQNERVLLAVKKVLNL"
BASE COUNT          745 a          710 c          747 g          712 t
ORIGIN      
        1 cctttccctt ttcttccggg attcgatgca ccgcaatcat ttctccgttg tccataaatg
       61 ccccaagctg gtaggtgtct tctatcccgt cgatggtgag cttgatttct ccccaatcga
      121 attctaccgt ccttcccgcc ggatgcactt ggatgactcg ttcttcgctt tctgggctga
      181 ttcggcttct ttctccgtta tgtagttccc aatagcgctt cagatacttc tttaccgtct
      241 ctttcgcgat ccctaacgct cgcgctgttc ctttaatcgt cgcgatctcg ttgtacgtct
      301 gtagaatcat tttgatttcc attgccgtct tcatcccctt cttcacctcc gagtgtattt
      361 tacttcctcg gaggcttttt tgtggggtca ttcttcgcgc gtttatcgcg gttattgttg
      421 ggtcgattgc atcccctggc tactctaggt cgaaaactga ctctcgtcgc ttatccttgt
      481 gggtcaagtg atgtggtgcc agcaagaaat aaggaaatat tcgatcgcgc gattcttaac
      541 ccacgaagta atcgcggcgt aagcgctttt tctttccgcg acagcgcgaa gagaaggggt
      601 tcacacggag ggcacagaga acacagagaa aagatttcta tgaacaccct gggaacgcct
      661 tgggaatgcc aaacttaagt ttggcaaggt tttatctttt ttaccggaaa ggttcctttg
      721 cgcagcatcg cggtgtaagc gctttttctt tccgcaaggg cgcgatacgc cgggaaagcc
      781 ggactttagt ccggcacgtt tttccgcgac actaacaaaa ggcaaagggc agatcgcggg
      841 ggcgcaaaaa ccacttaccc cgcgaaaaag aagcacggtc atcctcattc ctattttttc
      901 tatacctcga tcttatgctc gcacgcttat acttcttcaa gaaaagacat atctcacacg
      961 cattcctggt tgtacgatgc gattttttgt tcttgcttaa gaggatcgat cgttgtattc
     1021 ttcctccttt gacttagtgg ttaagataag agacgtgttc tcagcagagt gctttaattc
     1081 ggtctctttg aggatggcat tgaggacgat tccgatgacg ccggccaacc ccaatccttg
     1141 caaagagaag gagccgactg agaggttggc gccgccgatt ccgcacaaga gcataatcga
     1201 cataatcagc atgttctttg aagtaaaagc caccttgttc tccaccaaga cgcgaagccc
     1261 ggtggtggcg atcattccaa atagaacgat acacgccccg cccatcaccg gttcaggaat
     1321 ggccgtctga ataaaacggg tgagtgggtc gataaacgat agaaggatgg ccaggatagc
     1381 cgcgatacgc ataacccaag ggttgaactg tcgcgtcagc gccaatactc cggtattctc
     1441 ggagtaagtg gtgttcgcag ggccgccgat taaagcggct aaagaggtgg ctagtccgtc
     1501 ggcaaggatc gtacgatgga gtccggggtc cttgacaaac ggtttgttcg taaccataga
     1561 taccgcgtaa atgtccccga tgtgttccag ggccggagcg atggctgcgg gaaccacgat
     1621 ccctatggca tagagggaga acttaggcaa agagaataag gggaagctga accattggtc
     1681 gaaacggtac tcgggaacac cgaagaataa cgagacgacg tagcctacgg cgataccgat
     1741 caggataggt agcatagaga tgaaaccgcg ggtgtagagt ttgacgtaga tgatggtggc
     1801 gatcacgacg atggctaaaa cccagttctt ggatgcccag ttcacggcta caggggatag
     1861 gatcaagccg atcaaagaga tcatcgtccc ggtgacgaca ggcgggaaga ttttcgtgat
     1921 ccgatccgga cccaaatacc tgatgatcgc gctgaagatg acatacacga ttccggagac
     1981 cacgacacca cccaaggcat acgcgagggc gtcttccctg ccggcgtaat acgaagtgac
     2041 agcgatgatg ggagagatga aggcgaagga agagcctaaa tagaccggca ctttcccttt
     2101 cgtcagcaga tgaaagaaaa gtgtgcctac gccggacgta aacaacgcga cgctgacagg
     2161 gatccccgtt aaaatgggaa cgacgacggt tgcgccaaac atcgtgaaca agtgttgaag
     2221 acctaggaga aagagttgca tatacgacca cctccgttat ttttttgaac tgaagtattg
     2281 tatcacaaag ttcaggtata ttgttcccaa aaaaacgaaa aagatggtat aatttcatct
     2341 cataggaagt ggaggtcacc atgtctgcgc gcattctgat cgtggatgat tcattgttca
     2401 tgcgtacaca aatacgcaat attctctccg gtgccggata cgagatcgcg ggagaggcat
     2461 ccgacggaaa ccaggcggag gaaaagttta aggatctgaa tcccgacctc gtgattatgg
     2521 atatcacgat gcctggtacc aacgggatcg acgcgctacg caaaataaaa tctttaaata
     2581 gtcaagcgcg ggtgattatg tgcacgtcct tggggctaac cagtaaagtc aaagaagcga
     2641 tttccgaggg cgcagacgat tacatactca aaccctttca aaatgaacgg gttcttctgg
     2701 cggtgaaaaa ggtgctgaac ctctaacctt tgagctttca catccgggag agggggctct
     2761 ctctcgacgc gaaaacatcc tatcagcctt taaggaagat agaaaaaagc gcgtccgcct
     2821 caacgggcgc gcttttttcg atatattcga aggaagaaca acagccttcg gctggcgcta
     2881 atacctcggt cgctcgctta ggagcgttta tcta
//
LOCUS       DAOPXN010000234         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_35332_length_2912_cov_4.481624, whole genome shotgun sequence.
ACCESSION   DAOPXN010000234 DAOPXN010000000
VERSION     DAOPXN010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35332_length_2912_cov_4.481624"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PKM49_09635"
     CDS             complement(<1..519)
                     /locus_tag="PKM49_09635"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HNY83094.1"
                     /translation="MSIRERITTVKRLTAGAQSVAFGTVFIFGFTLVSKGLGFIREMV
                     LAAQFGTSWRMDAVIIAMEPAESLSGIIAGTLSTMMIPLYLEVKSGNDVEQTKRYASQ
                     VLKLAAGVLFLFSALLFFFPDLLIKVFAPNYSGEILEYAARKLRVLSVLPLIHGFGSI
                     FTAVLRAERRFVQ"
     gene            845..1789
                     /locus_tag="PKM49_09640"
     CDS             845..1789
                     /locus_tag="PKM49_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNY83095.1"
                     /translation="MRLAVVHDPFRSSDEQKMVETVANALSRNHETKMVVFGEDFIRM
                     VKAFDFVFNLSIWGGKEGRQVHVPAVLDVLGIPYTGSPVLTHSLCLDKILTKILLQKH
                     GVPTPAYAAFPVGRLPDRIDGKMFVKPSREGSAMGIHKDSLVSDLSRYREKVHSIHER
                     FGEPALAEEFIDGRELTIGFLGNGDTLEVLPILEIDFSGLPEGMERFYSYEVKHDFED
                     LTCFYCPARISDPLADKIRAGGKLAFEALGLRDYARMDVRLRGEDFFFLEVNSMPQLV
                     PGYSDLPKMAAVAGYSYEALIGKILDVAIERNRLSIDS"
     gene            2782..>2912
                     /locus_tag="PKM49_09645"
     CDS             2782..>2912
                     /locus_tag="PKM49_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNY83096.1"
                     /translation="MSERYILALDQGTTSTRSIIFDRSLRIVSVSQRATKQTYPKDG"
BASE COUNT          686 a          766 c          735 g          725 t
ORIGIN      
        1 ctggacgaat cggcgttctg cgcgtaaaac cgcagtaaaa atcgatccga agccgtggat
       61 gagcggtaaa acgctgagca cccggagctt tctggccgcg tattccaaga tttctccgga
      121 gtagtttggc gcgaagacct ttatcaggag gtccgggaag aaaaagagga gcgcggaaaa
      181 gaggaaaagc acgcccgccg ctaattttag gacttgggag gcgtatcgct tggtttgttc
      241 tacgtcgtta ccgcttttga cctcgaggta taacgggatc atcatcgtgc tcaaggtccc
      301 ggcaatgatc cccgaaagac tttcggcagg ctccatcgca atgatcacgg cgtccatacg
      361 ccaggaggtc ccgaattgcg cggctaacac catttcccgt atgaacccga gccctttgga
      421 tacgagggtg aatccgaaga tgaataccgt cccgaacgcg acagactgag cgccggcggt
      481 taaccttttt accgttgtga tccgttctct gatcgacacg cctatgaacc cctttgcgcc
      541 tttcatcata catcaagagt ataacaacca aatatcccga ggaaacgatc ctctgttccc
      601 gggcaactcg ttgagagaac cgtactcgct ttttcgatta aaccagaaat cttcgccgtt
      661 gacatcccct gccgttttga tatatagtgt tagaggactc catcctgggt tttgcaccat
      721 tttgggctct caaatcaaaa tgcacccggt ttttgcccag ttgccagctc gcggttggca
      781 aaacccgaat gaacccgccc acatcccccg ttaatgggca acctcaatcg gagatggtga
      841 tactatgaga cttgcggtcg ttcacgatcc ctttcgttcg tcggatgaac aaaagatggt
      901 ggaaactgtc gccaatgcct tatcccgaaa ccacgagacg aagatggtcg ttttcggaga
      961 ggacttcatc cgaatggtga aggccttcga tttcgttttt aacctgtcca tctggggcgg
     1021 gaaggaaggg agacaggtcc acgtgccggc agtcctcgat gtgttgggta tcccctatac
     1081 ggggtctcct gttttaacgc attctctctg cctggataag atcctcacga aaatcttgct
     1141 acaaaagcac ggggttccga cacccgctta cgcggctttt ccggtcggac gactcccgga
     1201 tcgcatcgac gggaagatgt tcgtcaagcc ctcccgcgag ggaagcgcga tgggaatcca
     1261 taaagactcc ttagtttctg atttgagccg ttatcgagaa aaggtccata gtatccacga
     1321 gcgtttcggg gaaccggcct tagctgaaga gtttatcgac ggacgggagc tgacgatcgg
     1381 gtttttgggc aatggcgata cccttgaggt gttacccatt ctggaaatcg atttctcggg
     1441 tttacccgag ggtatggagc gcttctactc ctacgaagtg aagcacgact tcgaggacct
     1501 cacctgtttc tactgtcctg cgcggatcag cgatccgttg gcggacaaaa tacgcgccgg
     1561 agggaagttg gccttcgaag ccttgggatt gcgcgattac gcccgtatgg acgtccgttt
     1621 acgcggagag gatttcttct tcttagaggt caattcgatg ccgcagctcg ttcccggcta
     1681 ctccgaccta ccgaagatgg ccgccgtcgc gggctattcc tatgaagccc tcatcggaaa
     1741 gattttagac gttgccatcg aaaggaatcg gctgtccatc gactcctgaa ccgctccttc
     1801 gaacttcgac tcgcttcata gcccgtgaga ccacctcgcc ttgtgggtat aatatcgagg
     1861 cgcaacaaaa aaatgaaata ggagtggctt ttcggcaatt tacaagacgt acttaattaa
     1921 catcttctat cgtaataact ctgtagaccc ttagccgttt cgaaaatccg cataatcgcg
     1981 taatcggcca cttgaccgtc tctttttatt tctattatgt tctttaatat ttctcggatg
     2041 atcagtgggc ttcaagtaat gtatcgaaga gccaaaaaac catgccaaac taaagtttgg
     2101 cgttcccagg cgttcacagc ccacgaagtc tcgcgatacg cctttgtgct taaccctaac
     2161 gccgtgaagc cgcgtatggg aacgttctca gtgaaaagat aaaaccgtgc caaactaaag
     2221 tttggcgttc ccaaggcgtg cctagaaaac ctgccgaact taagttcggc gtttccaagg
     2281 tgttccctga gtgttcacaa gaatctcttc tctgtgttct ctgtgccctc tggatgagtt
     2341 tttttcaagc catgccaact taaaagttgg cgttcccagg cgttcccaag gcgttcttag
     2401 aaaaccggcc gaacagaagt tcggcgttcc caggggaggc gcactgaagc catttcgagg
     2461 gtgttcacaa gaatctcttc tttgtgttct ctgtgccctc tgtgtgtgtt tttttttcaa
     2521 gacatgccaa actaaagttt ggcgttccca aggcgttctc aaggcgttcc caaggcgttc
     2581 ccagggcctt cgcagagaga caggccgact ggaagtctgc cccacgatga gatagggcgc
     2641 ttacgctgag atgactacat agactagaaa gcgcgcgatg gattccggcc tcgtttctcg
     2701 agtgtacctc ataacttgac aaacgccacc ccacggcctt aggggttttc cgtgctataa
     2761 tactcgaaaa cggagggctc tatgtccgaa cgctatatcc tcgcgctaga tcaaggcacg
     2821 accagtacga gatcgatcat cttcgacagg agtttgcgga tcgtttcggt ttctcagcgc
     2881 gcaacgaagc aaacctaccc aaaagatggt tg
//
LOCUS       DAOPXN010000235         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_35880_length_2880_cov_5.550088, whole genome shotgun sequence.
ACCESSION   DAOPXN010000235 DAOPXN010000000
VERSION     DAOPXN010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35880_length_2880_cov_5.550088"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(813..1583)
                     /locus_tag="PKM49_09650"
     CDS             complement(813..1583)
                     /locus_tag="PKM49_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HNY83097.1"
                     /translation="MNNSVENTTLLFGESKKVYERLWNPWHGCQKISPGCLHCYVYRM
                     DAQYERVSNSPTKNKTFDLPIRKRKNGSYFCPSGTLFWTCFTSDFLLDQCDEWRFEAW
                     RMIRERRDCRFLFLTKRIERFMVNLPDDWGEGYDHVSVCVTCENQETADRRLPILKEL
                     PIKHKMIANEPLLSPIDMRRYLDKTIESVVVGGESGPEARICRYEWVLDIREQCMYSG
                     VPFTFRQTGARFVKDGKLYRIPREIQHAQAKKAGIDIP"
     gene            complement(1590..2174)
                     /locus_tag="PKM49_09655"
     CDS             complement(1590..2174)
                     /locus_tag="PKM49_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNY83098.1"
                     /translation="MKEERDRGYIHIYTGTGKGKTTAAIGLAIRALGAGKRVLFAQFM
                     KTADFSEHRLLKTLDGLDLLTFGKPFFIASEDHREGAAQIDAQPVVFFAKGEPPSFYR
                     EWVKDGLREVEERLQGYALIVLDEILVALSFGLTTEEEIVALLQKTKVGQDILLTGRN
                     ASSGLIALADVVTEMVERKHYYRQGVRARVGIDE"
BASE COUNT          673 a          762 c          645 g          800 t
ORIGIN      
        1 tttttgtggg gtcacccttt gcgcgttgaa gggggttgtt tttgggtcga ttacatatca
       61 atcattttgg agtatagaga aataatatta aaaaatcaag tgttatttat ttacaattaa
      121 atagacattc tcttttgatc taaaataatt ctcttgtttt gttcttccat ctttctgtca
      181 acatctctga ttccagtctc gttctgactc atctttgctt tacagcgcaa aagcccgccg
      241 ctctcctcga gcggcggact tttgtctagc agatgtgggg gtttttcttt ttgggagcct
      301 ctatgaacgc cgcgcttagt gatttttccg gagaacccct tgccagtttt tacttattta
      361 ttctccgtgc cctctgtgct aacccttttt tagtctttgg aacgaacgaa aaccgagata
      421 tggcgccgtc gcgtaaagaa gatgcgctta cgccgcgatc ccggtttgga gttatcgcgg
      481 ggtaaccagc gggctgactt cggaagccca cgaaatctcg cgattcgcac ttgggaacgc
      541 cacactttag tgtggcatgt cttctacgtt ttcatctttt tccctgtcct ctgtaccccc
      601 tgcgcgaact tgatcatctg ttttttaatc taggcatttt cctttgtgtt ctctctgtgc
      661 cctctgtgtt ctccgtgcga acccagcttt tttcttttaa aagacctacc aacttaaaag
      721 ttagcgttcc cagaagatat gccgaactaa agttcggcgt tcccaagaaa cttttctcca
      781 aagctcggcc ttcccagaaa gagtccgatc ggctagggaa tatcgatgcc cgccttcttg
      841 gcttgcgcgt gctggatttc tcgcggtatt cgatagagtt ttccgtcttt gacgaaccgg
      901 gcgccggttt gccgaaaagt gaaaggaacg ccgctgtaca tacattgttc tcgaatatcc
      961 aatacccact cgtaccggca aatcctcgcc tcaggaccgg attctcctcc cacgacgacg
     1021 ctttcgatcg ttttatccag ataccgtcgc atgtcaatag gcgaaagcaa tggttcgtta
     1081 gcgatcatct tgtgcttaat cggaagctct ttcagaatcg gaaggcgtcg gtccgccgtc
     1141 tcttggtttt cgcacgtgac gcagacgctc acgtgatcgt acccttctcc ccaatcgtcc
     1201 gggaggttga ccatgaagcg ctcgatccgt ttcgtgagaa acaaaaaccg acaatcccgc
     1261 cgctctcgta tcatccgcca agcctcgaaa cgccactcgt cacactgatc caacaagaag
     1321 tcggaggtga agcacgtcca aaagagtgtc ccggaaggac agaaataaga cccgttcttt
     1381 cgttttcgta taggcagatc gaaggttttg tttttcgtcg gagaattgga aactcgttcg
     1441 tactgcgcat ccatccggta cacataacag tggaggcaac ccgggctgat cttttgacac
     1501 ccgtgccaag ggttccacag gcgttcatac acttttttgg attcaccgaa cagaagtgtt
     1561 gtgttttcga ccgaattgtt catcacaaac tactcatcta tcccgacacg cgccctgacg
     1621 ccctgacggt agtagtgctt gcgttcgacc atctccgtca caacgtccgc taaagcaata
     1681 agcccggaag aagcgttcct acccgtcaaa agaatatctt gacccacttt ggtcttctgt
     1741 aaaagcgcta cgatctcttc ttccgtggtc aacccgaagg atagcgcgac gagaatctcg
     1801 tccaagacga tcagcgcata cccctgtagc cgttcctcga cctctctcaa cccgtcttta
     1861 acccactctc gataaaacga tggcggctcc cctttcgcaa aaaagacgac cggttgcgcg
     1921 tctatctggg cggctccttc tctatgatcc tcactcgcaa tgaaaaacgg tttaccaaaa
     1981 gtgagaaggt ccagaccatc gagggttttc aacaggcgat gttcggaaaa gtcagccgtc
     2041 ttcataaatt gcgcgaacag tacccgtttt cccgccccga gcgcacgtat ggccaagccg
     2101 atcgccgccg tcgtcttccc tttgccagtc ccagtatata tatggatgta ccctctatcc
     2161 ctttcttctt tcaaatcgcc tctccttttt aatgttacgc aagactcttg gctcaaaagc
     2221 agcatcgctg atcgtcgcga gcgcaattct cctgctttca tctccgttgt caaatatgat
     2281 accatgctat gatacaccta aaggcgtttt cttgtggtgt gggtcaagct atggggtgca
     2341 atcaagaaac aaagcgagaa tcgactgtac gatttctgtc cttgtagtca tcgcggcgaa
     2401 agcgcttctt cttttcgcaa cggcgcgata cgcttttgta cgtgtcttga aaaacagggt
     2461 tcgcacagag ggcacagaga acacggagag agcaagcatc gcggcgaaag cgccccttct
     2521 tttcgcgacg gcgcgatacg cttttgtata tgtctcgaaa aacagggctc acacggaggg
     2581 cacagagaac acatagaaaa ggtattcgtg aacaccctgg gaacgccaac tttagtttgg
     2641 ctggttttgt cttttcactg ggagcgttcc cgaaggcagc aacgcggcgt aagcgcttct
     2701 tcttttcacg atggcgtgat acgctttccc tttttggaaa tctcgccttt acggtataat
     2761 accaatgaga cttttttctg gaggggatat ctatgaaaaa gatagataaa ccacacgaca
     2821 aattatttaa agaagttatg ggagatatcg aaacggcgaa gtccttcatc cagcactatc
//
LOCUS       DAOPXN010000236         2877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_35942_length_2877_cov_5.194897, whole genome shotgun sequence.
ACCESSION   DAOPXN010000236 DAOPXN010000000
VERSION     DAOPXN010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35942_length_2877_cov_5.194897"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            553..1110
                     /locus_tag="PKM49_09660"
     CDS             553..1110
                     /locus_tag="PKM49_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83099.1"
                     /translation="MKRLFRLWLLIALCLALVRCTTTLLLWDRSIAAGKEFLFTGDFE
                     RAIEYFQTAFSKSADEYQKSVSKAYEAWANIGVGNFPTAMSLLEESEELAHNEVSASG
                     RLYACYIQGDYTSLAEHTGDIYRIPSDFSLKLRGDTLTKTEIANLLMLGLAARYSKNE
                     FTNLKLILDGVYSDPLIDEMEAFFF"
     gene            1107..2723
                     /locus_tag="PKM49_09665"
     CDS             1107..2723
                     /locus_tag="PKM49_09665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNY83100.1"
                     /translation="MKKLFVLWLLCIPAIFLAIMPPRTPVEERIARGELSIPILTAEE
                     RGEAYHTPRAVGSFRAVVLLVDFSDQPAKVDTSFFHDLFLGRGVDFKAKYPISTNLKS
                     VQEYYEFVSDGQYQITFDIYGWFRLPETYAYYIGSNTGLGSYPRNAQRMTEDAVDMAD
                     SVVDFSKYDNDGDGWVDFLLIVHAGSGAEFEGDKNQIWSHQWTMSTTKTKDGKILYRY
                     CTQPEYWLQKDDMTIGVYVHELGHLLFNLPDLYDIYQNSKGIGYWGLMGSGSWNDSES
                     IFDRGSREVSGYGGAPAELTAWSKYKIGWLKPTEILANEGEYTLTRGTALKYTHPNNP
                     QEYFLIEYKEDNSYNRNMPSCEGLMIYHIDDGYSNNVFPWVPGDDPDLHYHVAIEQKD
                     GLWELERNLNRGTSDDLFFEGDAFSSITLPTSHFYDGRKGLSLIQIVIESDRAVISLN
                     EGTFVYFLMPIPGTEYYDVFIKKDRDALPAVIVSTTGERPAVTRIRESVFRFRIFREQ
                     YAFTRVDGTPLKNAPRATDATLFQNLRAVE"
BASE COUNT          734 a          682 c          771 g          690 t
ORIGIN      
        1 gttaaagaag catttaatgt agagagtccg acaaagggct tgaacacgat ttgatgggcc
       61 atatgtggga agtaatggaa tgtaatcaag aaacaggggc agaagagatc acgcgatttt
      121 ggtcggtgaa gccatcgcgg cgtgaataga caaagggtgg atcacgaggt cgtaaaaacc
      181 aatatcctgc gataacacac gatcagcgcg gcgtaagcgc cccttcttta cgcgacggcg
      241 cgatacgttc gggaacgact ttggaaagcc aactgttaag ttggcatgtc ttgaaaaaaa
      301 gctcacacgg agaccacaga ggtcacaaag aagagaggtg tttacggcag tcaacgccgg
      361 gtttatgttt cctctgagtt ctctgcgtac tctgtgcgaa accatctttt acttttttga
      421 atatgccgaa ctaaagttcg tcgttctgat tattgtgtac ctcgatacta tgccgcactt
      481 ttattaatac cacttcccga tctatgttat aatctgcaaa tcgagagata tcgatgtgaa
      541 cggaggtcaa agatgaaaag gctcttccga ttgtggctgt tgatagcgct ctgtctcgcg
      601 ctcgtccgat gtaccacgac cttgctgctt tgggacagat ccatcgctgc ggggaaagag
      661 tttttgttta cgggggattt cgagcgcgcg atagaatact tccaaacggc gttttctaag
      721 agtgcagacg agtatcagaa gtccgtttcc aaagcctatg aagcctgggc gaatatcggc
      781 gtggggaact tcccgaccgc tatgtccctc ttagaagaat cggaggagct cgcgcacaac
      841 gaggtgagtg cttccgggcg tctgtacgcg tgctatatcc aaggagatta cacaagcctc
      901 gcagaacata cgggggatat ctaccgtatc ccttcggatt tctctctgaa attgcgcggg
      961 gataccctaa ccaaaaccga aatcgcgaac ctattgatgc tgggattagc cgcgcggtac
     1021 tcgaaaaatg agttcaccaa cctcaaactg atcttggatg gcgtttattc cgacccgttg
     1081 atcgatgaaa tggaggcgtt tttcttttga aaaaactgtt cgtcctatgg ttattatgta
     1141 taccagcaat ctttctggcc atcatgccac cccggactcc tgtggaggag aggatcgccc
     1201 gcggagagct gtcaataccg atcttgaccg ctgaagaacg cggagaagcc tatcatactc
     1261 ccagagccgt cggctctttt agagcggtcg tgctcttggt ggatttttcg gatcaacccg
     1321 cgaaagtcga cacctctttc ttccacgacc tcttcctagg gcgtggcgtc gactttaagg
     1381 cgaaataccc catttcgacc aatctgaaaa gcgtccagga gtactacgaa tttgtctcgg
     1441 atgggcaata ccagatcacc ttcgatattt acggttggtt ccgccttccc gaaacctacg
     1501 cctattacat cggttcgaac acgggactcg ggtcatatcc tcggaatgcg cagcgaatga
     1561 ccgaggacgc cgtggatatg gccgattcgg ttgtcgactt ttcaaagtac gataacgacg
     1621 gcgacggttg ggtcgacttt ttgctaatcg ttcatgccgg cagcggcgcg gagtttgaag
     1681 gagacaagaa tcaaatctgg agccatcagt ggacgatgag cacgaccaag acaaaagatg
     1741 ggaagatact ctaccggtat tgtacgcaac cggagtattg gcttcagaag gatgacatga
     1801 cgataggcgt gtatgtgcac gaactcgggc acttgctttt caatctcccc gacctatacg
     1861 acatctatca aaattcgaag gggattggct actggggcct aatgggttcg ggaagttgga
     1921 acgacagcga atcgatcttc gataggggta gtcgagaggt gagtgggtat gggggcgccc
     1981 cggcggaatt gactgcctgg agcaaataca agatcggatg gctgaagccg accgaaatct
     2041 tggccaatga gggagaatac accctaacac ggggcaccgc tttaaaatat acacatccaa
     2101 ataatccaca ggagtatttc ctcatcgaat acaaagaaga caactcttat aatcggaata
     2161 tgccctcgtg tgaaggattg atgatctacc acatcgacga cgggtattcg aacaacgtgt
     2221 ttccgtgggt tcccggcgat gaccccgacc tccactacca tgtggcgatc gagcaaaagg
     2281 acgggctttg ggagttggaa agaaatctga accggggaac ctctgacgac ctgtttttcg
     2341 aaggagacgc gtttagcagc atcacccttc ccactagcca cttctatgac ggtagaaaag
     2401 gtctgtcgtt gattcaaatc gtgatcgaat cggatcgcgc ggtgatctcg ctcaacgaag
     2461 ggactttcgt ctacttcttg atgccgattc cgggtacgga gtattatgac gtgttcatta
     2521 aaaaagatag ggacgcgcta cccgcggtga ttgtgtccac cacgggggaa aggcctgcgg
     2581 tcacacggat acgggagtcc gttttccgct ttcggatttt ccgagagcaa tacgcgttta
     2641 cccgcgtaga cgggaccccg ctaaaaaacg cgccacgcgc cactgatgcg acgttattcc
     2701 aaaacctgag agctgtagag taaccgtgtg agtcaagtta tgaggtggta taactaagag
     2761 gttcatcgcg gggtaagtgg ttttgtaggg tcaagagaag gcgtcgtgaa gccagagctg
     2821 tgccgctctc gatttctgtg tgtgagtgtc catcacacca cctggcagct taccctc
//
LOCUS       DAOPXN010000237         2801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_37317_length_2801_cov_7.841952, whole genome shotgun sequence.
ACCESSION   DAOPXN010000237 DAOPXN010000000
VERSION     DAOPXN010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37317_length_2801_cov_7.841952"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /locus_tag="PKM49_09670"
     CDS             <1..1573
                     /locus_tag="PKM49_09670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNY83101.1"
                     /translation="LFSEAVLFYNSSLNPNELAGKRFAMVRGGTIPEGIREEDVLYFD
                     EREDTFEAVHDGKADYSYSNTYSLVFYALRSGYDNIITIPQKMEDRAYGVAVREDDEI
                     LLSIINKSIEAIDEEQRQSLILTALAEVERSVTLKEIFKRYGLPISLISVTVIAILTY
                     AVWLKVLANKHLNEEIQKGKRREEEIKYISFHDSLTGLYNRAYLKIVLESINDPENLP
                     LSVVVADLNGLKMFNDSFGHAAGDIILQKAATLLKSACRTEDVVARTGGDEFIIVLMR
                     ANRETALQVVNRIHRLCHNERAGQAFVSISVGYATKNEPERDLQEVVKEAETMMYKRK
                     LLESQSTRSAMLSALQATLQEKDIKTNESALRLIETASRLGERLQLSSTETVDLNLLA
                     RMLDIGYVVVDERILKKAGGLTDEEMAEVKKHPEAGFRIAESSRIAANIATYTLHHHE
                     WWDGTGYPHGLKGTEIPLLSRVISIADAYDAMTHDRPYRKAMSEKAAIAELERCSGTQ
                     FDPELVPIFISVVNT"
     gene            1770..2195
                     /locus_tag="PKM49_09675"
     CDS             1770..2195
                     /locus_tag="PKM49_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83102.1"
                     /translation="MKTALEIKTIIQTYEEVGTIKGTARALGIAKGTVKRYLKRYKEV
                     QNGERSRISPENEERVIVKMRNQEYDLRLKELIEQNEIAPKAKRLTMAKMHEILVAEG
                     WKVSYSTVKRRVREYEDYFKPRKVYIKQVPRAKRTIKSD"
BASE COUNT          832 a          651 c          713 g          605 t
ORIGIN      
        1 tctgttctcg gaagccgttt tgttttacaa ttcctcttta aaccccaatg aactggccgg
       61 aaaacggttc gcgatggtaa ggggagggac gatacctgaa ggtattagag aagaggatgt
      121 cctgtacttt gacgaaagag aagacacctt cgaagccgta cacgacggaa aagccgacta
      181 tagctattcg aatacgtatt ccttggtgtt ctacgccctg cgaagcggat acgacaacat
      241 tatcaccata ccccaaaaaa tggaagacag ggcgtatggc gtggcggtgc gtgaagatga
      301 tgaaatcctt ttgagtataa tcaataagtc catagaagct atcgacgaag aacaaagaca
      361 gagtctaata ctcaccgcgc tggcggaagt ggaacggtca gtgaccctca aggagatttt
      421 caaacgctac ggattaccca tttctctgat tagtgttact gtcattgcca tcctaacata
      481 tgccgtttgg ttgaaagtat tggcgaacaa acatctgaat gaagaaatcc aaaagggcaa
      541 aagacgagaa gaggagatta aatatatcag cttccacgac agtttgaccg gattatacaa
      601 tcgggcttac ctcaaaatag tccttgagag cataaatgac cccgagaatc tacccttatc
      661 cgtggttgtt gccgatctca atggtctaaa gatgttcaat gactctttcg ggcatgctgc
      721 aggagatata atcctccaaa aagcggcgac tctcttaaag agcgcttgcc gtacggaaga
      781 cgttgtggcc agaacaggcg gagacgaatt catcatcgta ttgatgcggg ctaatcgtga
      841 aacggccttg caggttgtga accgtattca tcgactttgc cataacgaac gtgccggtca
      901 ggcttttgtg agcatttcag taggctacgc cacaaaaaac gaaccggaac gcgatctcca
      961 agaagttgtg aaggaagctg aaacgatgat gtataaacgg aaattgctgg aaagccaaag
     1021 cacgcgtagc gcgatgttat cggccttaca agccaccctt caggaaaaag acatcaagac
     1081 gaacgagagc gctcttcgtc tcattgaaac agcaagcagg ctgggggaaa ggttacaact
     1141 ttcatcgaca gaaactgtcg acctgaacct tttggctcgg atgcttgaca tcggatacgt
     1201 cgtcgtagat gaacgcattt tgaagaaagc aggtgggctc acagatgaag agatggcgga
     1261 agtaaaaaag cacccggagg cggggttccg gatagcggaa tccagcagaa tcgcggccaa
     1321 tatcgcaaca tataccttac accatcacga atggtgggat ggcacgggtt atccccacgg
     1381 attgaaagga acggagatcc cgttgttgtc gagggtgatt tctatcgccg acgcgtacga
     1441 tgcgatgacg cacgacagac catatcggaa agcgatgagc gaaaaggccg ccatcgcgga
     1501 actggaaagg tgtagcggga cgcagttcga tccggaactt gtaccgatct tcatttcggt
     1561 ggtcaatact tgaaactcag tgcgtttaga catagaatga cttttcctct ccaaatgtgc
     1621 gcgcgtgcgt agattagaac cgagaatcga aaaaggcgcg caacactgtc tgtaaatggc
     1681 caaaaacaac tcgcttttcg ttcaaaaata gactcgtaaa aaatggacta ctgaggagga
     1741 aaaatacgct cggaggtgta tgaaagggta tgaaaacggc gctggagatc aaaacgatca
     1801 tacagacata cgaagaagtc ggaaccatca aagggacggc gcgagcctta ggaatcgcga
     1861 aagggacggt aaagaggtat ctgaaacgat acaaggaagt acagaacgga gaaagaagcc
     1921 gaatcagtcc ggaaaatgaa gagcgcgtca ttgtaaagat gaggaaccaa gaatatgacc
     1981 tgcggctcaa agaattgatc gaacagaacg agatcgctcc aaaggcaaaa cggttaacaa
     2041 tggcaaaaat gcatgagata ctggttgcag aaggttggaa ggtcagctat tcgacggtca
     2101 aacggcgggt acgggaatac gaagactatt tcaaaccgcg gaaggtatac atcaagcaag
     2161 tacctcgcgc aaaacgaacg ataaaatccg attgagcgaa acccgtttct atccctgata
     2221 aacgtagccc gaacagctgg attcccatac tctttcgttg cgggcggcgc ttgacccaca
     2281 caaatcacca gtgtttcaaa tagacgcctg gagcgctcag acaatctacc gagccccggt
     2341 ttcgcaaaga tacttttcga ccaccgcacg cccctttttt agagcgcaaa tccacgaagt
     2401 ctcaagcgga tacgagaaac tccaggacgc cctcggagtg cggaactttt gtgtctatag
     2461 aaaaaagaaa accgaaaaga cgtgccgaac gtagggtcaa gagaaggcgc cgtggagtca
     2521 gagatgcacc gctctcgatt tatgtgtgtg agtgtccatc acaccacctg gcagcttacc
     2581 ctctcccggt ttccttctcc tgcctcctca aaccgtacgt gcggatttcc cgcatacggc
     2641 tttccaaacg tcttcgtcaa aagggttatg tgacctttaa ccagtgagcg ctttcggtcg
     2701 gcgaggagcc tccttactcc acctctgcca gtcatccccg ttagtctggg tttcggcctt
     2761 cgccttttga tatagcttcg ccttccgttc cggcagctta t
//
LOCUS       DAOPXN010000238         2798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_37356_length_2798_cov_4.517681, whole genome shotgun sequence.
ACCESSION   DAOPXN010000238 DAOPXN010000000
VERSION     DAOPXN010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37356_length_2798_cov_4.517681"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..2198
                     /locus_tag="PKM49_09680"
     CDS             351..2198
                     /locus_tag="PKM49_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNY83103.1"
                     /translation="MQKKLLVLFACLLALTLFAFAAAKNPDTIVSAEYGSAESLDPHA
                     EWDNASGEVINNLYDNLIKYKGQTIEFEPMLSTAVPTVENGLLRDDGKTYIFPIRKGV
                     KFHSGNILSPRDVEYTFERGFLADNSSGAVTIIIEAVSGGAFYSIADWFEDYAGMPLS
                     EAFDDDRVPTSPEARAKMIAFYNEVIDPLFEVDGDNLIVHMRNPFAPFLSIIPKYAAW
                     GSILDSQWCIEHGEWDGKADGWWKWHDRTPEESALHMKDAGCGPYKLAVWDQTNGMVV
                     MERFDDYWQGPAKTKTIIFKAVVEYSTRKALLEAGDADVILVDPIYRYDFDNNPDIQV
                     ISGHPRAQVTSIHMGWRVKETSEYIGSGKLDGKGIPPNFFTDINARAGILHSFNYDAL
                     IDDLVNGLGKRVPTDLPEGFLGYDPTLPVYAFDLSKAEKAWKAAWNGEVWKNGFKLVA
                     MYNAGNEVRQTACEMLKVYVESINPKFQVEVLGVQWPTYLKATREMLCPLYIVGWVAD
                     YPDSHNFFSTYYHSKGYYGARHGEDYAAFAVKYLDPIIDAAVIESNVSKRAELYAQLQ
                     KILLDNYIAAPLYMPQGLFVARKWLKGFFPHLIRSSGDSTPEFYYAWKE"
     gene            2279..>2798
                     /locus_tag="PKM49_09685"
     CDS             2279..>2798
                     /locus_tag="PKM49_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNY83104.1"
                     /translation="MLAFIIRRLLILPIILFGVTLIIFMMIWMLGPERLIGTYVKSPE
                     ALKSEKSVERLIQKYGLDRPAPEMYFKWLGNVLKGDFGYSNVGRQGVLQSILELIPYT
                     FELALYALIPVVFVGIWLGVIAGAHQDKLLDHGIRIFAIVGWSLPDYVFGLLVLLIFY
                     SYLKWFPPGFLSD"
BASE COUNT          697 a          710 c          706 g          685 t
ORIGIN      
        1 gtttcatcat atcataaatc taccaaaatc ttggaatttt cgaccggctt aaacgggggc
       61 tttagacatt tggtgtgtgt caagttatga ggtgtaatca agaaacaggg cttgaatcga
      121 tcgcacgatt ttttgtccat gcagtcatca gagggtacgc gcttaataat tctcggaagc
      181 ttctagaaga gcgaaaaacc tgccgaacta aagttcggtg ttctcatttt ggcgaaccct
      241 gatattacac ccaactcggc atttgctctt ttgcaaaaat gtgctataat ttgtaatcac
      301 agtccaccca tcccgtgagg gatgaaaacc cgaaaggagg aattattttt atgcagaaaa
      361 agcttcttgt tctctttgcc tgtcttctcg cccttaccct tttcgctttc gcagctgcga
      421 aaaatccgga caccatcgtt agcgctgagt atggctctgc cgagtcgcta gacccccatg
      481 ccgaatggga taatgccagc ggcgaagtca tcaacaacct ctatgacaac ttgatcaagt
      541 acaagggtca aaccatcgag ttcgaaccga tgctttcgac cgccgtcccg acagtcgaga
      601 acggtctttt gagagatgac ggaaagacct acattttccc gatccggaaa ggtgtgaagt
      661 tccacagcgg gaatatcctt tccccgagag acgtcgagta cacgttcgag cgggggttcc
      721 tcgccgataa ctcgtcggga gccgtgacga ttatcatcga agccgtgtcc ggcggtgcgt
      781 tctactccat tgcagactgg ttcgaagatt atgccgggat gcccttaagc gaagcgttcg
      841 acgatgacag agtaccaact tccccagagg cacgagcgaa aatgatcgct ttctacaatg
      901 aggtcatcga ccccttgttt gaagttgatg gcgacaattt aatcgtgcat atgagaaacc
      961 ccttcgcgcc tttcctctcc atcatcccca aatacgctgc ctgggggtcc atcctcgata
     1021 gtcaatggtg tatcgagcat ggcgaatggg atggaaaagc ggacggttgg tggaaatggc
     1081 acgatcgcac tcctgaagaa tctgcgttgc acatgaaaga cgcgggttgc ggtccttaca
     1141 aactggcggt ttgggaccaa acaaacggta tggtcgtgat ggaacgcttt gacgactact
     1201 ggcaaggacc cgccaagacc aagaccatta tcttcaaggc cgtggtagag tactccacca
     1261 gaaaagcgct gctcgaagcc ggagacgccg acgtcatcct cgtagacccg atctaccggt
     1321 acgacttcga taacaaccct gatatccaag tcatcagcgg ccacccgaga gcccaagtga
     1381 cgtctatcca tatgggttgg agagttaagg aaaccagcga atacatcggc agtggaaaac
     1441 tcgatggcaa aggtatcccc cccaacttct tcaccgatat caacgcccgc gcggggattc
     1501 tgcactcctt caactacgac gccctcatcg acgatttggt caatggatta ggaaaacgcg
     1561 taccgaccga ccttccggaa gggttcttgg gttacgatcc cacgttgccg gtttatgcgt
     1621 tcgatctatc aaaagctgaa aaagcctgga aagccgcttg gaacggagag gtttggaaaa
     1681 acgggtttaa gctggtcgcg atgtacaacg ccggtaacga agtacgacaa acggcttgtg
     1741 agatgctgaa ggtttacgtc gagtcgatca acccgaagtt ccaggtcgaa gttctcggcg
     1801 tgcaatggcc aacttacctc aaggcgaccc gcgaaatgct ctgcccgctc tatatcgtcg
     1861 gatgggtcgc ggactacccc gactcgcaca acttcttctc cacctactac cactcgaagg
     1921 gatactacgg tgcgcgacac ggtgaggatt acgccgcctt cgctgtgaaa tacctcgacc
     1981 cgatcatcga cgccgctgtg atagaatcca atgtgagcaa acgcgctgaa ctgtacgccc
     2041 agttgcaaaa aatcctacta gacaactaca tcgcggcacc gttatacatg ccccagggct
     2101 tgttcgtggc cagaaagtgg ttgaaaggct tcttccctca tctcataagg tccagcggag
     2161 acagtacccc cgaattctat tacgcctgga aagagtaacc gaacgtttgt attttcgtaa
     2221 cgcgagggcg ggatcgattc ccgcccttaa ttggtatata cactgagagg tggaaaaagt
     2281 gctggctttt atcattcgga gattgctgat ccttccgatc attcttttcg gtgttacgtt
     2341 gatcatcttt atgatgatat ggatgctcgg cccagaacgg ttgataggaa cctatgtgaa
     2401 aagcccggaa gctttgaaat cagaaaagtc ggttgaacga ctgatacaga aatacgggct
     2461 ggatcgcccc gctccggaaa tgtattttaa gtggcttggg aacgtcctca aaggggattt
     2521 tggttattcg aacgtcggga gacaaggcgt tcttcaaagc attttggaac tgattcccta
     2581 caccttcgaa ttggctctct atgccttaat accggtcgtc ttcgtgggta tctggttggg
     2641 ggtgatcgcg ggggcgcacc aggacaaact gctggatcac ggcatcagga tcttcgccat
     2701 cgtcggctgg tccttgcccg actacgtctt cggcttgttg gtgctcttga ttttttatag
     2761 ttatctaaaa tggtttccac ccggtttttt gtcagacg
//
LOCUS       DAOPXN010000239         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_37963_length_2767_cov_4.801254, whole genome shotgun sequence.
ACCESSION   DAOPXN010000239 DAOPXN010000000
VERSION     DAOPXN010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37963_length_2767_cov_4.801254"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1867)
                     /locus_tag="PKM49_09690"
     CDS             complement(65..1867)
                     /locus_tag="PKM49_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HNY83105.1"
                     /translation="MQIDGLTLSKVVLELRGFLMNQRVNKVFMPSEGRFYFTFFRDTL
                     LVSLLNDASYLQMVPTKEDSPFFPPAFVMLLRKYLKGAACTDISQLRHDRIIRIDFLS
                     HFEEAEPKEYIVYIELMGRNSNLILCEKSGKILDVYRRKASPQRGLLPQNTYVPFSKN
                     PPPLSLVEWDILKGSELLEEEIRLFPSDLPIFAFLERGFDGFGHQTSAEICYRADLAP
                     ERSLSALSRTEIENLNLVLSSLREELSNTPGLYLWESDEKACVLSAIRMQTLEQKGFR
                     YTIKPPSEALQEVFSISRQMRDLQISKARYRKWIDKEIKKIESNIDSMQSDLSETVNY
                     PSYQRYGQLILANQYRFDSDKKYTSLEAEDWETGESVLVPLDSKLTLSANAQVYFKKS
                     SRLRRRGEVLGDRLRRFQSLLYYLEQIQQSLRDVEDPEALSDIILEIERENRLFGEDT
                     RVEQTKKSGASKQAKEKRHAFTTRYSGPSEYRVFERSGFRCLVGKNNYQNDRLIKEAK
                     DGDLWFHVQGFPGAHVILKSRNQPFVPEEVLLFAARLAARYSKASQSANVAVDYTDMK
                     DVKKPKGFRPGMVTYRNHKTLFVDPYYDDESDRL"
     gene            complement(1886..2641)
                     /gene="deoC"
                     /locus_tag="PKM49_09695"
     CDS             complement(1886..2641)
                     /gene="deoC"
                     /locus_tag="PKM49_09695"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HNY83106.1"
                     /translation="MSDFDLKIESAVASFLRNYHFEEKTIEKEFARTFDEQVEQKIEH
                     TLLKSIATTSEIEKLCHEARQYGFRSVCVNPSFVSLADDLLTGTGVLVVTVVGFPLGA
                     TMTNCKEYETYQAMQNGANEVDMVIHQGRLRSKDYECVLHDIRKVVKAAGSNPVKVIL
                     ETCNLTKEEIIAGCLLAIEAGAQYVKTSTGFGAMGAKAEDIDLMRHVVGKQIGVKASG
                     GIKDRASALRMAAYGADLIGTSSGVAILKECKG"
BASE COUNT          642 a          720 c          672 g          733 t
ORIGIN      
        1 agaaagtggc ttttatcgcc gtttgtagca cttttagtcg aaagttgggc tgggactctc
       61 tgccttataa acgatcgctt tcatcatcat agtagggatc gacgaaaagg gttttatggt
      121 ttcggtaggt caccattccg ggtcgaaacc ccttcggctt cttcacatct ttcatatcgg
      181 tatagtcgac cgccacattc gcgctctggg aggctttcga gtaccgggcc gccaacctgg
      241 ccgcgaagag taaaacctcc tccgggacga acggctgatt tctacttttc aagattacgt
      301 gggcgccggg aaatccttga acgtgaaacc aaaggtctcc atccttcgcc tctttgatca
      361 aacgatcgtt ttgatagttg ttcttcccga cgagacaacg aaagcccgat ctttcaaaaa
      421 cgcggtattc agacgggccc gaatagcggg ttgtgaaggc gtgtcttttc tctttagcct
      481 gtttcgacgc gcccgacttt ttcgtctgtt ctacgcgcgt gtcctcgcca aagagacggt
      541 tctctcgctc gatttcgaga ataatgtcgc ttagcgcttc cgggtcctcg acatcgcgaa
      601 ggctctgttg gatttgctct aaatagtaaa ggagggattg gaatcttcgg aggcgatcac
      661 ctaagacttc gccccttctt ctcaagcgtg aagacttttt gaagtaaact tgggcgttgg
      721 ccgatagtgt aagcttcgag tccaatggaa ccaatacgga ctcgccagtc tcccaatcct
      781 ctgcctctag ggaagtgtat ttcttgtcgc tgtcgaaacg gtattgattg gccaagatca
      841 gctgtccgta tcgctgatag gaagggtaat ttacggtttc agacaagtcc gactgcattg
      901 agtctatgtt cgactcgatc tttttgatct ctttgtcgat ccactttcga taacgcgctt
      961 tagaaatctg taaatctcgc atctgcctgg aaatggagaa aacttcttgg agagcttccg
     1021 agggcggctt gatcgtatag cggaagcctt tttgctcgag ggtttgcata cgaatcgcgc
     1081 tcaacacaca cgctttttcg tcggattccc aaagatagag gccaggtgta ttggacaatt
     1141 cttcgcgcag agaagacaac acgaggttca ggttctctat ctccgtgcga cttaaggctg
     1201 aaagggatcg ttcgggcgct aaatccgcgc gataacaaat ttcggcagat gtttggtggc
     1261 cgaagccatc gaaacctctc tccaggaacg cgaaaatcgg caggtccgaa ggaaacaagc
     1321 ggatctcctc ctcaaggagt tccgaccctt tgagaatatc ccattctacc aaagataacg
     1381 ggggtgggtt cttcgagaaa ggcacatagg tgttttgcgg taacagcccc ctttgcggag
     1441 aagccttcct acggtagaca tcgagaatct tcccgctctt ttcgcacaga ataaggttgg
     1501 agttgcgtcc cattaactcg atataaacga tatactcctt gggttcggct tcttcgaagt
     1561 gactgaggaa atcgatgcga atgatccggt cgtggcgtag ctgagagata tcggtacagg
     1621 ccgctccctt cagatatttc cgtaagagca taacgaaggc gggcgggaaa aacggactat
     1681 cctctttcgt cggaaccatc tgcaggtaag acgcatcgtt cagcagagaa accaatagag
     1741 tgtcacgaaa aaaagtaaaa tagaaccggc cttccgaagg catgaagacc ttattgactc
     1801 tttggttcat caaaaatccc cgaagctcga ggacgacttt ggatagagtc aaaccatcta
     1861 tttgcataga ctcacccgaa ggcgtttacc ctttgcactc tttgaggatt gcgacccccg
     1921 aactcgttcc tatcaaatcc gctccataag cagccattcg taatgcgctc gcgcggtcct
     1981 tgataccgcc ggaggctttc acgccgatct gtttcccgac aacgtgtctc atcagatcga
     2041 tgtcttccgc ttttgccccc attgcgccga aaccggtcga tgttttgaca tattgcgcac
     2101 ccgcctctat agcgagcaga caccccgcga tgatctcttc tttagttaag ttgcacgttt
     2161 ccaggatcac cttcaccgga ttcgaacccg ccgctttcac gactttccgt atatcgtgca
     2221 gcacgcattc gtagtctttt gaacgcaatc tcccctggtg gatgaccata tcgacctcgt
     2281 tcgctccgtt ttgcatcgcc tgataggttt cgtactcttt gcagttggtc atcgtcgccc
     2341 ccaatgggaa accgacgacg gtcaccacga ggacgccggt cccggtaagc aaatcgtcgg
     2401 ctaaagagac gaaggagggg ttgacacaaa cggagcggaa cccgtactgt cgagcttcgt
     2461 gacagagttt ctctatttcg ctcgtggtag cgatgctttt caatagcgtg tgttcgatct
     2521 tctgctcgac ttgctcgtca aaggttcgcg cgaactcctt ttcgatcgtc ttttcctcga
     2581 aatgatagtt tcttagaaaa gaggcgacag cgctttcgat ttttaggtca aagtccgaca
     2641 tcgtgacctc ccatacactc attcgctcca agaaggatag aatttaattt cgattccgct
     2701 ttatcggctc ggctcacgat caggataggc gctttcgccc cgatcacgat ccccccaatt
     2761 gggaaac
//
LOCUS       DAOPXN010000240         2763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38050_length_2763_cov_4.442024, whole genome shotgun sequence.
ACCESSION   DAOPXN010000240 DAOPXN010000000
VERSION     DAOPXN010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38050_length_2763_cov_4.442024"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(226..1929)
                     /locus_tag="PKM49_09700"
     CDS             complement(226..1929)
                     /locus_tag="PKM49_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83107.1"
                     /translation="MMDSQGSATVDVFKFLSQNQNPMNWVVFSTGGRLELQGSGNIWG
                     LAGTNATTLLPSGQRPVYFDSGAYIATPTQNFYIGPNAVSTQIAYIAAGSGNNYQTTL
                     RELIERDQVKKLTAPVVYAVPSCFLALNFQPVYTETTKITSMYKAIDGQNVLTYLLNS
                     GNFSLVRLNANQTVRIVVGDSDMVLNISELIMGGNSKIEIEKLSPASMGNVFLMVSKM
                     TLDSTAKILPLDGNPDKVFLFYSGSQDVTFGVGSEYYGSVYTSGPSKITFSGRGVLRD
                     VLALEGSVTLNSANMTLGGLYIKSGEATVIGATLLTGILSGGTKVAISGGVPTFSFIF
                     APSAVVTIENNTGINAKPATYETVKGIVVGKEVIIKDSRVVGPAYQLPLCLGIPVPVI
                     EEEVYIEPSKDCSSGGWYVETSGQICQASPNVRTSSQPAIIGAKGVQYGGQKTISNNP
                     NEIVMTWKAPYIEFLSPLALMTSLKLESKYFIFNNYLGLYKNDVRVTLIPAVGGESIV
                     QFGNVYSSSNNQSQDRRMEISNTAFRFNKELIITPSTTVNSLKAQGAMLYEGTIAVEF
                     Y"
     gene            complement(2137..>2763)
                     /locus_tag="PKM49_09705"
     CDS             complement(2137..>2763)
                     /locus_tag="PKM49_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83108.1"
                     /translation="FKNEYQDYVESTIEQAPFNEPVCCTDFDLGFAFIEVDEEENIGN
                     KETAEVSEDTYYQGGLYVGNGGTLKILSGTSEDVTVLLISSLTVKGDIVIEGNGLVII
                     KVLETLIVEGDVYWNTFPNVNPGSEGDPKNLLILYDGSEYAQNGGRINGYLGFSDSVT
                     SVKLAGNMILNGALYAPKATVEVNGTVGVSQWMYGTTFDIKGTVNVYP"
BASE COUNT          691 a          726 c          616 g          730 t
ORIGIN      
        1 tttaggtcgg catgggtttt ttctccaaac caaacgaaaa ggaacaataa aagaaggggt
       61 ctgcccgact aaagatcggg cgctccaacc ctcgagtact ccaaccatct gcgatccaat
      121 cgtcgggcgg tccatttcgt ctacgcaaaa aaaagcgctc caagaatcgg gctcgccgtc
      181 tctaacccgt atcgtgggct tctattatct ctatcttccg aaatgtcaat aaaactccac
      241 ggcgatggtg ccttcgtaca gcatcgcgcc ttgagctttc aacgaattca ccgttgtgct
      301 cggggtaatg atcaattctt tgttgaatcg aaaagctgta ttggaaattt ccatccgccg
      361 atcctggctt tgattgtttg agcttgagta aacatttccg aattggacga tcgattcccc
      421 gcctacggcc ggtattaagg taacccggac atcgttttta taaagcccca gataattgtt
      481 gaagataaaa tacttcgatt ccaacttgag ggaagtcatc aacgcaagag gactgaggaa
      541 ctcgatatag ggggccttcc aagtcataac gatctcgttc gggttattgc tgatcgtttt
      601 ctgccctccg tactgaaccc cctttgctcc gatgatcgcg ggttgcgaag aagttcttac
      661 attcggcgag gcttgacaga tctggccgct cgtctccaca taccaacccc ctgaagagca
      721 gtctttggaa ggttcgatat agacttcctc ctcaatcacg ggaacaggaa tccccaagca
      781 caggggcagt tggtaggcag gcccgacgac ccgggaatcc ttgatgatga cttctttccc
      841 gacgacgatg cctttaacgg tttcataggt agccggtttc gcgttgattc cggtgttatt
      901 ttcgatggtg acgaccgcgg aaggagcgaa gataaaactg aatgtgggaa cgcctcccga
      961 aatagccact ttcgttccac ccgacaatat ccccgttagg agtgtggcgc cgattacggt
     1021 cgcttcccca gacttaatat acaggcctcc gagagtcata ttggcgctgt tcaacgtgac
     1081 cgaaccttcc agcgcgagga catctcttaa gacacctctc ccggaaaacg tgattttgga
     1141 cggccccgag gtatagaccg aaccgtaata ctcgctcccg actccgaagg tgacatcttg
     1201 agaaccggag taaaacaaga atactttatc cggattccca tctaagggga ggatcttggc
     1261 cgtgctatcc agcgtcatct tcgagaccat gagaaagacg ttgcccatac tcgcgggaga
     1321 aagtttttcg atctctatct tcgaattgcc ccccataatc agttcgctga tattcagcac
     1381 catatcgcta tccccgacga cgatcctcac cgtttggttg gcgttcagcc gcacgagaga
     1441 aaagttgcca ctgttcagga gataggttag aacattttgc ccgtcgatcg ctttgtacat
     1501 cgaagtaatc ttggtcgttt ccgtatacac cggctgaaag ttcagcgcca gaaaacatga
     1561 aggaaccgcg tagaccacgg gagccgtcag ttttttcact tgatctcttt ctatcaactc
     1621 acgaagcgtc gtttgataat tgttacccga acccgccgca atataggcga tctgcgtcga
     1681 taccgcattg ggtccgatat aaaagttttg cgtcggggtc gcgatatagg cgcccgaatc
     1741 gaaataaacg ggccgttgtc cactcggtag aagtgtggtt gcattggtgc ccgccaaacc
     1801 ccaaatgttc cccgatccct gtaactctaa ccttccgccc gtcgagaaga ccacccaatt
     1861 catcggattc tggttttgag agagaaactt gaaaacatcc accgtcgccg atccttggga
     1921 atccatcaca cggatatagt tgtcctcttc cgaaaacccc attacgacca gtaagaaaag
     1981 cgataatacc aaaaaaacga ataaacggcg aatagtcttt cctccttcct agaattcgag
     2041 aaactcagga atgccttatg gtattgtttt tcgaaaacaa gttatttcgt taataaaacc
     2101 ggcttacgcc ggttttatga gccaaataat cgttttttaa ggataaacgt ttacggttcc
     2161 tttgatatca aaagttgtgc catacatcca ttgactaacc cctacggttc cattcacttc
     2221 gacagttgct ttgggggcgt acaaagcgcc attaaggatc atgtttcccg ccaattttac
     2281 tgaagtcacg ctatcgctga atcccagata accgttgata cgtcccccgt tttgggcata
     2341 ctccgatccg tcgtacaaaa tgagcaaatt tttcgggtcg ccttcactgc cgggattgac
     2401 gttcgggaac gtgttccaat atacgtcccc ttccacgatc aaggtttcca atactttgat
     2461 aatcactaaa ccgttccctt cgatgacgat atctcccttg acggtgagtg acgatatcag
     2521 caagacggtt acatcttcgc ttgttccgct taggatcttc agggtccccc cgttgccgac
     2581 gtacaatccg ccttgatagt aggtatcctc gcttacctct gccgtttctt tattaccgat
     2641 attctcctct tcatccactt cgatgaaagc gaagccgaga tcgaagtccg tacaacaaac
     2701 cggttcattg aatggggctt gttcaatcgt gctttccacg taatcctggt attcgttttt
     2761 gaa
//
LOCUS       DAOPXN010000241         2758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38147_length_2758_cov_4.824639, whole genome shotgun sequence.
ACCESSION   DAOPXN010000241 DAOPXN010000000
VERSION     DAOPXN010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38147_length_2758_cov_4.824639"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PKM49_09710"
     CDS             complement(<1..313)
                     /locus_tag="PKM49_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNY83109.1"
                     /translation="MEDRRKPSKEKASRPIPPDEANMPKFIISVAAKMLQLHPQTLRQ
                     YEKRGYVTPFRLGNLRLYSERDIHTINRIKELADEGIPTNGIDRILALERRIEELERL
                     LE"
     gene            complement(363..2207)
                     /gene="dnaK"
                     /locus_tag="PKM49_09715"
     CDS             complement(363..2207)
                     /gene="dnaK"
                     /locus_tag="PKM49_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNY83110.1"
                     /translation="MAKEFVVGIDLGTTNSVIAWVKPDKTSEVISNAEGSRTTSSVVS
                     FSKTGEIIVGEPAKRQAVLNSDRTIRSIKRKMGTDTKNTIDGKDYTPQEISSYILRKM
                     VKDAEAYLGGTIKKAVITCPAYFNDAQRQATKEAGQIAGLEVLRIINEPTAAALAYGL
                     DRKNDEKVVVFDLGGGTFDVSILEIGDGVIQVVSTNGNNHLGGDDFDKRVVDWLADNF
                     RKAHGVDLRNDRQALQRLYEAGEKSKIELSTKMETDISLPFITATAEGPLHLEAKLTR
                     ATLESLVRDLVESTRGPVENALRDAKLNASDINEVLLVGGMTRMPLVQQTIKAIFKKE
                     GNKSINPDEAVAVGAAIQSSILSGEMGKDVVLVDVTPLTLGVEVKGGLLEPIIERNTP
                     IPVKKAKVFTTAEDNQTEVEVRVYQGERKIATQNHMLGSFRLVGIMSAPRNIPQIEVS
                     FDIDTDGIVNVNAKDLGTGKEQGMVVTGRNKLDPTEINKMVEDAKRNEEEDNRRKQEI
                     EMKNQADHLVYSIEKILRESGDKIPGDLRSKVESLKNDLRDAVNKENMQRIKVLMDAL
                     NLESMKMGAFLYKQPNASPDPNDVQTGGQSGPNPEDGNTYTAEYEDVQ"
BASE COUNT          538 a          759 c          663 g          798 t
ORIGIN      
        1 cctctaaaag cctttctaat tcctcaatac gtctttccaa cgccaggatc cggtctatgc
       61 cattggtcgg tataccttca tcggcgagtt ctttgattcg attgatcgta tggatatctc
      121 tttctgaata gaggcgaagg tttcccaacc gaaaaggggt gacataaccc cgtttctcgt
      181 actgtctgag cgtctgcggg tgcagctgca gcatcttggc ggccacgctg atgatgaact
      241 tcggcatatt ggcctcgtca ggggggatgg ggcgggatgc cttctcctta ctcggtttac
      301 gcctatcttc caattcgatc gcctcctttc aaaagaaagc gggctgtacg cccgccttcg
      361 ttttattgca catcttcgta ttcagcggtg taggtgtttc cgtcctccgg attagggcca
      421 ctctgcccac ccgtttggac gtcattcgga tcgggactcg cgttaggttg cttatacagg
      481 aatgcgccca ttttcatcga ctccaggttc agcgcatcca ttagtacttt gatccgctgc
      541 atgttttctt tgttcaccgc atcgcgcagg tcgttcttca gcgactccac cttcgaacgg
      601 aggtctccgg ggatcttgtc gccgctttcc ctcagaatct tttcaataga atagaccaga
      661 tggtcggctt ggtttttcat ctcgatctct tgttttcggc gattgtcttc ctcttcgttc
      721 cgtttggcgt cttcgaccat tttgttgatc tccgtcggat ccagcttatt ccttccggtc
      781 acgaccatgc cctgctcttt gccggttccg agatctttcg cgttcacgtt cacgataccg
      841 tccgtatcga tatcgaagga tacctcgatc tgtgggatat ttctcggagc ggacataatc
      901 ccgaccagtc ggaaactacc cagcatatgg ttttgtgtgg cgattttgcg ttctccttgg
      961 taaacgcgca cctccacttc cgtctggttg tcttcggctg tggtgaagac cttcgccttc
     1021 ttgaccggaa tcggcgtatt cctctcgata atcggttcta ataaaccgcc cttcacctcg
     1081 actcccagcg ttagtggggt gacgtccact aagacgacgt ctttacccat ctcgccggag
     1141 aggatcgaag attggatcgc ggcgccaacg gcaaccgctt cgtccgggtt gatgctcttg
     1201 ttgccttctt tcttgaagat cgccttgatc gtttgttgga ccaagggcat ccgggtcatc
     1261 cctccgacga gtaacacctc attgatgtcg gaagcgttca gtttcgcgtc acgtaaagcg
     1321 ttttcgacgg gcccccgagt gctttccacc aaatcgcgga ccagcgattc gagcgtcgcc
     1381 cgagtgagtt tcgcctccaa atgcaacgga ccttccgcgg tcgcggtgat aaaaggcaga
     1441 ctgatatccg tctccatctt cgtggaaagt tcgatcttcg atttctcgcc agcttcgtaa
     1501 aggcgttgga gcgcctggcg gtcgttccgc aaatcgacgc cgtgcgcctt tctgaagtta
     1561 tcggccagcc aatccaccac acgtttgtcg aaatcgtcac cgcccaaatg gttgttcccg
     1621 ttggtcgaaa cgacctggat cacgccatcc ccgatctcca gaatcgagac gtcgaaggtt
     1681 ccgccgccta agtcaaagac caccactttt tcgtcgttct tgcggtccag tccataggct
     1741 aaagccgcgg ctgtcggctc gttgatgatg cggagaactt ccaaacccgc gatctgtccg
     1801 gcttcctttg tggcttgccg ctgggcgtcg ttaaagtatg cgggacaggt gatcaccgct
     1861 tttttgatcg tgccgcccag gtaagcctcg gcgtctttga ccattttcct cagtatgtag
     1921 gagctgatct cttgaggcgt ataatctttt ccatcgatcg tgttttttgt atcggttccc
     1981 atcttcctct tgatcgaccg aatcgttcga tcggagttga ggaccgcttg acgtttcgcg
     2041 ggttctccga cgatgatctc ccctgttttg ctaaaagaga cgacggaaga agtggtccgc
     2101 gacccttccg cgttcgaaat cacttccgat gtcttatcgg gtttcaccca cgcgatgacc
     2161 gagttggtgg tacccaagtc aatacccact acaaattcct ttgccatagt gcacctccac
     2221 tatatttttt aatttgagat tatagacgtt attccatctc tatttattcg agatagacgc
     2281 tctcttctaa gtgcgtgtat attatatact tttaggttct taatgtcaat agtttattta
     2341 ttaactaact tattctgttc aaccgtcatt tattgattct cacgaaatca tttacgctct
     2401 tgagtgtgcg tagaatatcg aagcgcaacg aaaaatagaa agaggtggag atcagccttt
     2461 tggccttttt acgccgcgat gggcctgcgg ggttatcgcg gagtaaccaa tgagacactg
     2521 cgattcgccc tttgcccctt ctacgccgcg atgactatgc agttcatcgc ggggtaacca
     2581 gcgggctgac ttcggaagcc cacgaagtct cgcgatacta acgaaatcca gcaacgcgct
     2641 ctttcctatc tttagcttct aagacctgcc aactgtaggg tcaagagaag gcgccgtgga
     2701 gttagagatg taccgctctc gatttctgtg tgtgagtgtc catcacacca cctggcag
//
LOCUS       DAOPXN010000242         2757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38170_length_2757_cov_5.466691, whole genome shotgun sequence.
ACCESSION   DAOPXN010000242 DAOPXN010000000
VERSION     DAOPXN010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38170_length_2757_cov_5.466691"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PKM49_09720"
     CDS             complement(<1..655)
                     /locus_tag="PKM49_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNY83111.1"
                     /translation="MAPMRSFDELLASVKQRVQAVGMKKVVLCGAEDREGLLSVREAL
                     QKGIASPVCVGKRSLIEPLLEELGIPTVELIAADSEEETAFQSVLCVRQGNGDFILKG
                     SVKTSTLLKAVLHKETGIRKEPLLSHTLVTEMTHQQRFLLITDGGMVIRPTLEEKIAI
                     IGNAVKVARSLGMMVPKVACICAVEVVNPAMPESVDSAILSKMNQRGQIKDCLIEGPV
                     "
     gene            complement(659..>2757)
                     /locus_tag="PKM49_09725"
     CDS             complement(659..>2757)
                     /locus_tag="PKM49_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83112.1"
                     /translation="HFAEDQNQLFTYHWDKTLRVFRVYDLKEIAQIQTNTMILGFEID
                     ASGRSVAIQTEDKTIYLGIQGSENLPGFIDRYEELLPLDKNQVATEPERPTILAQTAP
                     ATQSTVSVQDPVQPASTVTEPPIAVQTPMIAVAPLVESEAERNVPPTMSQLYGYWEYG
                     AAYRDMGYFVASSGCVAWLNLILDTTARYELQPGVIRDLDISRNGLVGLLLEDRIELW
                     DAKYMAENQKVTTYKGMKYALKDGVRLTFSRTGRKILIVLYDGRVTMLNSDLTGQMVL
                     ESKINATAICPDLTQTDAFVIGTDKGELIWLSENGIYQRESVSDQAISEVLFAYGDIY
                     WGDRSGNMGVFKKKTMRLEGGYVTSLYAPENGKNWIVVGSSTGNLYIYANEFSRLMGK
                     ANLGETPVTGIHGYMDTMLTVNEDRSLRSWNVSQIGVLTTKPPLRHSLGMYSEQNLLG
                     FVTTDGNNFIIDTEKGSMLPRKLDIGTSRVEQFHKKPPVIRVNDNFYSIQKNTLSEKS
                     YKAYENDKLNTADKFLLFWSGDLLKVIDLETGSTIRTLKFGEPNVVTWGTMVNKRLIF
                     FLEGKMGIVNPYKPEDLLIVDVADEKIGKILEVFIYQDRLIVVDDNGLIHQYGMMESK
                     FLTPLNLTQKLTKVYYNSARLQLIGYYGNRIIRFSPVDNDIQVDYVQGVIKDIDWTDR
                     SFFALTEDGILWGKGF"
BASE COUNT          593 a          771 c          667 g          726 t
ORIGIN      
        1 ccaccgggcc ctctatgaga cagtccttga tctggcctcg ctgattcatt ttgctgagta
       61 tggccgagtc gacgctttcg ggcatcgcgg gattgacgac ttcgacggcg cagatacacg
      121 cgacctttgg gaccatcatc cctaagcttc gcgccacttt caccgcattt cctatgatgg
      181 cgatcttttc ttctagcgtt ggtctgatca ccattcctcc atccgtaatc agtaaaaacc
      241 tctgttggtg cgtcatttct gtcactaggg tatggctgag caacggctct tttcggatac
      301 ctgtttcttt atggagcacg gctttcagta aggtggaggt cttcaccgaa cctttcagga
      361 tgaagtcccc gtttccctgc cgaacgcata gaacgctttg aaaggccgtt tcttcttctg
      421 aatccgcggc gatgagttcg accgtgggta tcccgagctc ctctagcagc ggctctatca
      481 gagatcgctt ccccacacaa acgggagagg cgatcccttt ttgcaacgct tcgcgcacgc
      541 tgagcagccc ctctcggtct tcggcgccgc aaagcacgac cttcttcatt ccgaccgctt
      601 gtacacgttg tttcacgctg gccaacagtt cgtcaaaaga acgcatcggg gccatctgtt
      661 aaaaaccctt tccccaaagt atcccgtctt ccgttaacgc gaagaaagac ctgtccgtcc
      721 aatcgatatc cttgatcacg ccttggacgt aatcgacctg aatatcgtta tccacggggg
      781 aaaagcggat gatccggttg ccataataac cgatcaattg caatcgagcg gagttgtagt
      841 agaccttcgt caacttttgc gtcagattga gcggggttaa aaacttgctc tccatcatcc
      901 cgtattggtg gatcagccca ttgtcgtcga cgacgatcaa ccgatcctga tagataaaaa
      961 cctcgagaat ctttccgatc ttttcgtcgg ctacgtcgac gatcaacaag tcttcgggtt
     1021 tatagggatt cacgatcccc atcttcccct ccaggaagaa gatcagccgc ttattgacca
     1081 tcgtccccca ggtcacgacg tttggctcgc cgaacttcag ggttcgaatc gtcgatccgg
     1141 tttccaggtc gatgaccttc aagaggtcgc cggaccagaa gagcagaaat ttgtccgctg
     1201 tgttcagttt gtcgttttcg taagccttat aagacttttc gcttagcgtg tttttttgga
     1261 tggagtagaa gttgtcgttg acgcggataa ccggtggttt tttatggaat tgctccacgc
     1321 ggctggttcc aatatcgagc tttctcggca acatcgaccc cttctccgta tcgatgatga
     1381 agttgttccc atcggtcgtc acgaaaccca gaagattctg ctcagagtac atacccaacg
     1441 agtgccgtag tggcggtttt gtggtgagca cgccgatttg acttacattc caactccgca
     1501 aagagcggtc ttcgttgacg gtcagcatcg tgtccatata cccgtgtatc ccggtaaccg
     1561 gtgtttctcc caagttcgct ttccccatca atcgggaaaa ttcgttggcg tagatgtaca
     1621 agttcccggt actagagccc acaacgatcc agtttttccc gttttccggc gcatagaggg
     1681 aggtgacgta accgccctcc aaccgcatcg tcttcttctt aaaaacgccc atgttcccgg
     1741 atctgtcgcc ccaatagata tcgccgtatg cgaagagcac ttcggagatc gcttgatcgg
     1801 agaccgactc cctctgatag atgccattct cagaaagcca gataagctct cccttatccg
     1861 ttccgatcac gaacgcgtct gtctgggtta agtccggaca tatcgcggtc gcgttgatct
     1921 tcgactccaa taccatctgc ccggttaagt cgctgttgag catcgtgact ctgccgtcat
     1981 acagcacaat caggatcttc cgcccggttc tagaaaaggt caatctcacc ccatccttta
     2041 gggcgtactt cattcctttg taggtcgtca ccttttggtt ttcggccata tatttggcgt
     2101 cccacagctc aatacggtct tccagtaaaa gtcctaccag cccgtttcta gagatgtcca
     2161 gatcacgaat aacgcccggt tgcagctcgt aacgcgccgt cgtatccagg atcaggttga
     2221 gccacgcgac gcagccgcta gaggcgacga agtaacccat atcacgatag gcggcgccat
     2281 actcccaata cccgtataac tgactcatcg tcgggggtac gtttcgttcc gcttctgact
     2341 ctactaaggg ggcgaccgcg atcatcgggg tttggacagc tataggaggc tccgtgacag
     2401 ttgaagctgg ttgaacgggg tcttgcacgc tgactgtcga ttgcgtggca ggagcggtct
     2461 gcgccaagat cgtaggtcgt tccggctcgg tagcaacctg attcttgtct aaaggaagga
     2521 gctcttcata ccggtcgata aacccgggga gattttcgga cccttgtata cccagataaa
     2581 tcgttttatc ctccgtttga atcgccacgg acctgccact cgcatcgatc tcgaagccca
     2641 aaatcatcgt gttcgtctgg atttgagcga tctctttcag atcgtaaacg cggaaaacgc
     2701 gcagcgtttt gtcccagtga taggtgaaca gctgattctg atcttcggca aagtggc
//
LOCUS       DAOPXN010000243         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38355_length_2747_cov_4.629643, whole genome shotgun sequence.
ACCESSION   DAOPXN010000243 DAOPXN010000000
VERSION     DAOPXN010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38355_length_2747_cov_4.629643"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKM49_09730"
     CDS             <1..319
                     /locus_tag="PKM49_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNY83113.1"
                     /translation="PERFRIPGTSEYIQGVEVPWRYSGTLPAGYDILELPACKMMMFQ
                     GPPFVDEDFREAIGEVEEAIERFEPGVFGYEWAYEDGPRVQAAPVGSRGYLEGRPVRK
                     KTK"
     gene            complement(423..1988)
                     /gene="rny"
                     /locus_tag="PKM49_09735"
     CDS             complement(423..1988)
                     /gene="rny"
                     /locus_tag="PKM49_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNY83114.1"
                     /translation="MDVILWILLAIGAFCFGGWIGLFYYRKFLTEKTREELKKEFVEA
                     NQSAEEIKKDAKREAEDIVKNARLDAKEEAHKIKEEALTEKNRQREELKKTESKLDKR
                     DEALSLREEQLIKREEQVRNKENSVNEKEKEIEQLVNAQKEELLRIAGMTEDEAKEIV
                     FDHTRRRYEHDIAQMFKEIKDTYADEADRQAKWVVSTAIQRHASEYVNELAVNAVPLP
                     SDDMKGRIIGREGRNIRAFEKITGTDLIIDDTPEVVVISCFNPLRREIARLTLEKLIV
                     DGRIHPANIEEMYEKARNEIIKQIKEEGQNALFETGIKGLHGELTKLIGRLKFRTSFG
                     QNVLEHVIEVSKLAALMASELGLNVERAKRGALLHDIGKAVDHEVDGSHAMIGSELAK
                     RYGEKEDIINMIQYHHNETEATCPESVIVAAADALSAARPGARRETVDLYIKRLEKLE
                     SIANEYGNVEKAFAIQAGRELRIIVIPEKVDDAIADQMAFEIAKKIEEEVQYPGQLKV
                     TVIREKRSVAYAN"
     gene            complement(2020..2466)
                     /locus_tag="PKM49_09740"
     CDS             complement(2020..2466)
                     /locus_tag="PKM49_09740"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNY83115.1"
                     /translation="MNDAIKAIDDYARILFRYRIRTEWETRERCAEKGFDSESIEALI
                     ERLKERGLLDDDRFLRMYLYEGLTLKKKGIYVLREELSRLGIARDQIMEAWRALSKRE
                     DIPALLAQWASKEKSFDPNRWKTKMLKRGFESEDIGQALEVLEDRD"
     gene            complement(2459..>2747)
                     /locus_tag="PKM49_09745"
     CDS             complement(2459..>2747)
                     /locus_tag="PKM49_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012057372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HNY83116.1"
                     /translation="APPFREAMFDILYGKGIVRENELVTVGVETEIIQKKGSWFSYVN
                     SQGVETSLGQGKNAAVDYLVNNPAVAEEIEQRVRAKHGLRKPSSGEIQTDE"
BASE COUNT          515 a          787 c          633 g          812 t
ORIGIN      
        1 gcccgaaagg ttccgaattc ccggcacttc ggagtacatc caaggggtgg aagtgccctg
       61 gcgctattct ggaaccttgc ctgcgggata cgacatcttg gaacttccag cctgtaaaat
      121 gatgatgttt caaggtcccc ctttcgtaga cgaggatttc cgagaggcga taggcgaggt
      181 ggaagaggcc atcgaacgct ttgaacccgg ggtcttcgga tatgaatggg cgtacgagga
      241 tgggccgagg gtgcaagcgg cccccgtcgg atcgcgcggg tatctcgaag ggcgcccggt
      301 tagaaagaaa acgaaatagc gccccttcag agataaaaaa cggccaaacc gtctggaatc
      361 ccccaggcgg ttagccgttt tgtctatccc gaggtcgctg agtccccggt actcggcgta
      421 ccctagttgg cgtaagcgac cgagcgtttt tctcgaataa ccgttacctt cagctggccg
      481 gggtactgta cctcttcttc tatttttttc gcgatttcga aggccatctg atccgctatc
      541 gcgtcgtcca ccttctccgg tatcacgatg atgcgcagtt ccctcccggc ttgtatggcg
      601 aaggcttttt cgacattccc gtactcgttc gcgatgcttt ctaatttctc caaacgcttg
      661 atgtacagat cgacggtttc gcgcctcgcc cctggcctgg ccgcggataa cgcgtcggca
      721 gcggcaacga tgaccgactc cggacacgtg gcttcggttt cgttatggtg gtattgaatc
      781 atattgatga tatcttcttt ttctccgtac cgttttgcga gctctgaacc gatcatcgcg
      841 tgagagccat caacctcgtg atcgaccgcc ttaccgatat cgtgtaacaa ggcgccgcgc
      901 ttcgcccgct ctacgttcaa ccccaactcg ctcgccatca gcgccgctag tttcgagacc
      961 tctatcacgt gttccaagac gttttgcccg aagctcgtac ggaatttcaa gcgtccgatc
     1021 aatttcgtca attctccgtg taggcctttg atgccagtct cgaacagcgc gttttgtcct
     1081 tcctctttga tctgcttgat gatttcgttt cgggcttttt cgtacatctc ttcgatattc
     1141 gccgggtgta ttctcccgtc gacaatcagc ttttccaacg tcaatctggc aatctcacgt
     1201 cgaagtgggt tgaaacagga gatgacgacc acctcagggg tatcgtcgat gatgaggtct
     1261 gttcccgtaa ttttctcgaa cgcgcggata ttccgacctt cccgcccgat aattcgaccc
     1321 ttcatatcgt ccgaagggag cggtacggcg ttgaccgcca attcgttgac gtattcggac
     1381 gcgtgtcgtt ggatggccgt cgacacaacc catttggctt gccgatccgc ctcgtccgca
     1441 taggtgtcct tgatttcctt aaacatctgc gcgatatcgt gttcatatct tctcctcgta
     1501 tgatcaaaca cgatttcttt cgcttcatcc tcggtcatcc ccgctattcg gaggagctcc
     1561 tccttctggg cattgaccaa ttgttcgatc tctttttctt tctcgttcac gctgttttct
     1621 ttgttccgca cctgttcttc tcgtttgatc agctgttctt ctctcaagga tagagcttcg
     1681 tcccgtttat ccaatttgga ttcggttttc ttcagttctt cccgttgacg gttcttttcg
     1741 gtcaacgctt cctctttgat tttgtgcgct tcctctttgg cgtcaagccg cgcgttcttg
     1801 acgatatcct ccgcttcgcg tttcgcatct ttctttattt cttccgcaga ttgattcgct
     1861 tcaacgaact ctttcttcag ttcttctcga gttttttctg ttaaaaactt gcggtaataa
     1921 aaaaggccta tccatcctcc gaagcaaaac gcgccgattg ccaggaggat ccataagatc
     1981 acatccattg tgccacctcc atatatataa gccaccatat tagtcgcggt cttcgagcac
     2041 ctccaatgcc tggccgatat cttctgactc gaatccgcgc ttgagcattt tggttttcca
     2101 cctattcggg tcgaaggact tctccttcga cgcccattgc gcgaggagcg cgggtatgtc
     2161 ttctctcttc gatagggctc gccacgcttc cataatctga tctcgcgcaa tccccaaccg
     2221 acttaactct tctcgtaaaa cgtatatccc ttttttcttc aatgtgagcc cctcgtagag
     2281 atacattcgg aggaaacgat cgtcgtcgag gagtcctctt tctttcaacc tttcgatcaa
     2341 ggcctctatc gactcgctgt caaatccttt ttccgcacat ctttctctag tttcccactc
     2401 cgtgcggatg cgatatctaa aaagaattcg ggcgtagtcg tctatggcct taatcgcgtc
     2461 attcatccgt ctggatttct ccggacgatg gctttcgcag tccatgcttc gcccggacac
     2521 gctgttcgat ctcttcggcg acagcggggt tatttacgag gtagtccaca gccgcgtttt
     2581 ttccttgacc taagctggtt tccacgcctt gggagttaac gtagctgaac caggatcctt
     2641 tcttctgtat gatctctgtc tctaccccta ccgtgacgag ttcgttctct ctcacgatgc
     2701 cttttccgta aaggatatcg aacatagcct ctcggaaagg gggcgct
//
LOCUS       DAOPXN010000244         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38843_length_2723_cov_4.838831, whole genome shotgun sequence.
ACCESSION   DAOPXN010000244 DAOPXN010000000
VERSION     DAOPXN010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38843_length_2723_cov_4.838831"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1807
                     /gene="preA"
                     /locus_tag="PKM49_09750"
     CDS             296..1807
                     /gene="preA"
                     /locus_tag="PKM49_09750"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HNY83117.1"
                     /translation="MEKETFRRLATLEEVNRCLLCLDAPCRAACPSGLEPDRVIRSIR
                     FENLNGARNRLSEDLPCATCEARECQTACLKGKINRPIDIPLVLREARSFQEYRTDEP
                     SCSLDFCGVWCENPFFLSSSVVGSDYEMIAKAFEMGWAGVVYKSISAFVPKDTSPRFG
                     VLRAENGEIIGLKNIEQTSNRSLSENLSILARLKRDFPRKVVVASIMGRNEEEWTQLA
                     QAVTATGIDMIECNFSCPHMAADGVGSDVGQNPDLVKAYVRAALKGTRLPILAKMTPN
                     TGNMEISALAAFEAGATGLAAINTVKSLMGIHPDTFSSDPDVGGKSSVGGYSGKAVKP
                     IALRFIQALKSHEKAKNAPISGMGGITTWRDAMEYLAVGCENVQVTTAVMLYGYRIIE
                     DLIEGILGFLASQGYKNLSDVIGKALSHVVSGEDLDRDSLQFPRFDKERCVGCGRCAV
                     SCDDGGHQALRLDGSTRQPILDPARCVGCHLCIAVCPTLAITPAARFRRDRFR"
     gene            1850..>2723
                     /locus_tag="PKM49_09755"
     CDS             1850..>2723
                     /locus_tag="PKM49_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNY83118.1"
                     /translation="MIRWIIKKIIKDYTATSNKRVREAYGRLAGALGIGCNLLLFVMK
                     LAIGIKMNSIAILADAFNNLTDLGSSTVSLLAIQISNRPPDKEHPHGHGRFEYISTLV
                     VSFIIFGFGFSLLSNALQKIGSPEPISFDPVGLTILLISVLVKLWMFSYNRYIGKTIG
                     SNVYRAAAADSLNDVLATLTVVAAMVFGSSTTAPLDGIIGVGISAFILWTGFTIAKDT
                     VNVLLGSSPSPELVRDIRVLIPKDQYIWGTHDLVVHDYGPGRVLASIHVEVPDTANIV
                     EIHSVIDDLERKISK"
BASE COUNT          608 a          762 c          748 g          605 t
ORIGIN      
        1 ttcctgctga acatttccgc cttatctgga cgtactaaag ttcggcgttc ccagggcgtt
       61 cccaaaaaat taaaaaaccg gcggaactga agttcagcct tcccagaagg gtgctccatc
      121 aaagacgttc ccagccggcg caccccgcgt ccgctcccaa gcgcttccag gacatatgac
      181 ccgtgggctc atgattgaac ctctataagc cattggcgga aatatcgccc caagaacgaa
      241 aagagcgtat aatcccggat agcgcaccac acttttctac acttggggga ttaagatgga
      301 aaaagaaacc ttccgccggc tcgcgacctt agaagaagtc aaccgttgtt tgctctgttt
      361 ggacgcgcct tgtagggcgg catgcccttc cgggctcgaa ccggaccggg ttatccgttc
      421 gattcgattc gaaaacctta acggagcgcg aaaccgcctg tcagaggatt taccttgcgc
      481 gacgtgcgaa gcacgcgagt gccaaacggc ttgcttgaaa ggaaagatca accgaccgat
      541 cgacatcccc ctcgtcctgc gggaagcccg ttcctttcag gaatatcgga ccgatgaacc
      601 gagttgttcc ctggatttct gcggcgtgtg gtgcgaaaac cccttcttcc tttcttcctc
      661 cgtggttggc agcgactatg agatgatcgc caaagccttc gagatggggt gggccggggt
      721 agtgtacaaa agcatcagcg ccttcgtacc gaaagacacg tcacctcggt ttggggtgct
      781 tcgagcggaa aatggggaaa taatcgggtt gaagaacatc gagcaaacct cgaatcggtc
      841 tctgtccgag aacctctcca tcctcgccag actcaaacgg gacttcccgc gtaaggtcgt
      901 cgtcgcctcg attatgggcc ggaatgagga agagtggacc caattggccc aagcagtgac
      961 cgcaaccggt atcgatatga tcgaatgtaa cttttcttgc ccccatatgg ccgccgatgg
     1021 cgttggttcg gatgtggggc aaaaccccga tctcgtgaag gcttacgttc gggcggcgct
     1081 caaaggcacg cggttaccga ttctggcgaa gatgaccccg aataccggaa acatggaaat
     1141 ctccgcctta gccgcctttg aggccggagc gacggggctt gccgcgatca atacggtcaa
     1201 aagcttgatg ggcatccatc cggatacgtt ttcgtcggac ccggacgtcg gcggaaagtc
     1261 cagcgtgggt ggctattccg gaaaggccgt gaaaccgatc gccctgcgct tcatacaagc
     1321 cttgaaatct cacgaaaagg cgaaaaacgc cccgataagc gggatggggg ggatcacgac
     1381 ttggcgggac gcgatggaat acctcgcggt gggatgcgaa aacgtccagg tcacaacggc
     1441 ggttatgctc tatggatacc gcatcatcga agacttgatc gaaggcatac tcggttttct
     1501 cgcttctcaa ggctacaaaa acctgagcga cgtcatcggc aaagccctct cccacgtcgt
     1561 ctccggagaa gacctcgacc gggatagcct acagttcccg aggttcgata aggagcggtg
     1621 cgtcgggtgt ggacgctgtg ccgtctcctg tgatgatggc ggtcaccaag cgcttcgtct
     1681 ggacgggagc acgcgccaac cgattctgga cccggctcgt tgtgtggggt gtcatctctg
     1741 tattgccgtt tgcccgacgc tagccatcac ccctgccgcg cgctttcggc gggatcgttt
     1801 ccgttaaccg aacccgctgg gtagttttct ttttaaggag gtgcctgagt tgatccgttg
     1861 gatcatcaaa aaaatcataa aagactacac ggccacctcg aacaaaaggg tgagggaagc
     1921 ctatggacgg cttgccggcg cgttggggat aggctgcaat ctcctgcttt tcgtgatgaa
     1981 gttggcgatc ggcatcaaga tgaacagtat cgccatcttg gccgacgcct tcaacaacct
     2041 taccgacctg ggctcctcca cggtgtctct gctcgcaatc caaatcagca accgcccccc
     2101 ggacaaggag cacccgcacg gtcatggtcg gttcgagtac atttcaacct tggtcgtgtc
     2161 gttcatcatc ttcggattcg gtttcagtct gctttcaaac gcccttcaga aaatcggaag
     2221 cccggagccc atttcgttcg accccgttgg attgaccatc ttactgattt ccgttctcgt
     2281 gaaattatgg atgttttcct acaaccggta cattggaaaa actatcggat cgaacgttta
     2341 tcgcgcggcg gcagccgata gtctgaacga cgtgctcgcc acactcaccg tggtcgcggc
     2401 gatggttttc gggtcttcca caaccgcacc gctcgacggc atcatcggcg tgggtatatc
     2461 cgccttcatc ctttggaccg gatttaccat cgcgaaggac acggtcaacg tgttgcttgg
     2521 ttcctctccg tccccggaat tggtaagaga catccgggtg ctaataccta aagaccagta
     2581 tatttgggga acccacgatc tggtcgttca cgactacgga ccggggcggg tgctcgcctc
     2641 gatccatgtg gaggtgccag atacggcgaa catcgtggag attcattcgg tgatcgatga
     2701 tctggaacgt aaaatttcca aac
//
LOCUS       DAOPXN010000245         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_38881_length_2721_cov_3.226557, whole genome shotgun sequence.
ACCESSION   DAOPXN010000245 DAOPXN010000000
VERSION     DAOPXN010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38881_length_2721_cov_3.226557"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            827..1912
                     /locus_tag="PKM49_09760"
     CDS             827..1912
                     /locus_tag="PKM49_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNY83119.1"
                     /translation="MRVADIIQYEGDNKTLVWKHPTQDFNTTTQLIVHESQEALFFKN
                     GQALDLFPSGRYTLETQNIPLIKRILNLPTGGKTPFHSEVYFINKTNILNVKWGTSTR
                     AEVFDPKFQILLNVGASGAMGISVGDARKFVVKLVGTETGFSLEQLSVYFKELISTRV
                     KAYLARIMNEVSFVVVNSHLNDISFAVKNQLSEDMQEYGILLPNFFISTIHVPDEDKQ
                     KVKEALSTASARAIEGYNWVDEQISEIAKKYATRTGTSGQGIDWIAQAPVAFAFGQML
                     KESMHPFMNRTFANTAKAFGESRENPTSGAVEGKKNCPKCGAVSPLKAAFCSQCGFQY
                     PEKLCSKCGNKINDGDKFCSQCGTPTN"
     gene            1931..2626
                     /locus_tag="PKM49_09765"
     CDS             1931..2626
                     /locus_tag="PKM49_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83120.1"
                     /translation="MPEKRNERLLIIVPIILIALYNLIVFSVVRNYTPFFWTNYLFTL
                     FSFLLFILIVGKFAFMDGIAIKDIFFGIPLLYLSWTYLAAQIIISALMMALPILPLTP
                     LTIFYIILLGLYLILAVSAVYGKKEIYRTAREATEKVTFIRTLSESIERTRMGVSDAQ
                     IKKRLGDLGEAVRYSDPMSHPSLSSIETVLSVKIDELSKIVPSESPDRILPLLEELSR
                     LVAERNAKCKYLK"
BASE COUNT          712 a          635 c          684 g          690 t
ORIGIN      
        1 taagtggttt ttacgacccc gcgatccccc ttttgtattt tttgcgccgc cccgggagcg
       61 cctaggaacg ccggacttta gtccggcatg ccttttcttt ttccgcgaga acactgtcct
      121 ttgccatttt ttcctctgtg ctctctgtgc cctctgagcg aaccctgttt tgatctttgg
      181 aacgaatgaa aggggtatcg cgccgtcgcg aaaaaaaaaa gcgcttacgc cgcgatgccc
      241 gtgtggggtt atcgcagggg aaccagcggg ctgacttcgg aagcccacaa agtcccgcga
      301 tatgcctttt gtctttttta ctccgcgatg actacattga ctataaaacg cgcgatcgat
      361 tccggcctta tttcttgatg gcgcctcata gcttgactcg tgcctttcag agaggtgaga
      421 gattccttat gatatactat cttattatga actgtctcac ttttagggca tatcgattat
      481 gcctagtatt acggcgacgg gttggaacga gcccaatgca aaatcagatt tgatagaatg
      541 tgagctatgg cgctataaca agatagagcg gacgccgggg ctttaaagtg gcttgaaacc
      601 gatttacgct gcccttttct ttgcgtatcg gctttttcgc ccgttttcac gggcttccag
      661 tgaacttttc tctttatcgg ttcgaaatgg tgaacgaatc ataagaaatt tgcgatacgg
      721 aaagtggcgt ttgattcagt aaatatagag taaggtaggg aaccgtgcgg cgggaacgac
      781 ggttttatgg aaacagatga aacatcgatc ggggaggtaa tcgagaatgc gggtagcgga
      841 cattattcaa tatgaagggg ataataagac cttagtgtgg aagcatccca ctcaggattt
      901 caacacgacc acgcaattga ttgtacacga atcacaggaa gccctattct ttaagaacgg
      961 gcaggcttta gacttgtttc catctgggag atacacgcta gaaactcaga acatcccgct
     1021 cattaagcgg attcttaacc ttccgactgg cggaaagacg ccttttcatt cggaggtcta
     1081 ttttatcaac aaaaccaata tcctcaacgt caagtggggg accagtacgc gagcggaggt
     1141 tttcgacccc aaattccaga ttctgctgaa tgtcggcgcg agcggcgcga tgggcatcag
     1201 cgtgggcgat gctcggaaat tcgttgtgaa actggtggga acggaaacgg gattctccct
     1261 cgaacagcta tcggtttact ttaaagagct tatctcgacc cgggtcaaag cctatctcgc
     1321 caggattatg aacgaagtca gttttgtcgt cgtcaattcg catctgaacg atatctcgtt
     1381 cgctgtgaaa aatcaactct cggaagatat gcaggaatat ggaatccttc ttccgaactt
     1441 tttcatctcg acgatccacg tacccgatga agacaaacaa aaagtgaaag aggcgctctc
     1501 aacagccagc gcccgagcga tcgaagggta caactgggtc gacgagcaga tctcggagat
     1561 cgccaaaaaa tacgcgacaa gaactggaac gagcggccaa gggatcgact ggatcgcgca
     1621 agcccctgtc gcgtttgcgt ttggtcagat gttgaaagaa tcgatgcatc cgtttatgaa
     1681 ccgaacgttt gcgaatactg cgaaagcttt tggggaatcg cgcgagaatc caacatcggg
     1741 ggcggtcgaa ggaaagaaga actgcccaaa atgcggggcc gtctcccctt taaaggcggc
     1801 attttgttct caatgcgggt ttcaataccc agaaaagctg tgttcgaaat gcgggaataa
     1861 gataaacgat ggcgacaagt tctgttcaca atgcggaacg ccaacaaact gagaaaggcg
     1921 ggggataccg atgccggaaa agcgaaacga aagactgctc ataatcgtac cgatcatatt
     1981 gattgcgctc tataacctga tcgtttttag tgtggtccgg aattatacgc ccttcttctg
     2041 gacgaattat ctctttacct tgttttcatt cctgcttttt attctgatcg tcggaaagtt
     2101 cgcgtttatg gacgggatcg cgattaagga cattttcttc gggattcctc tgctgtatct
     2161 ctcttggacc tacctggccg cgcaaatcat cataagcgcg ttgatgatgg cgttgcctat
     2221 cttacccctg accccgctga cgattttcta tatcatcctt ttggggctgt atcttatcct
     2281 cgccgtttcg gcggtttacg gaaaaaagga aatctaccgg acagcgcggg aagcgaccga
     2341 aaaagtaacg ttcatacgaa ccctctccga atccatagag agaacaagaa tgggcgtatc
     2401 cgacgctcag ataaaaaagc gattgggcga tttgggggaa gcggtgcggt atagcgatcc
     2461 gatgagccac ccatcgcttt caagtatcga aactgttcta tcggtaaaga tcgatgagtt
     2521 gtcgaaaatc gttccttccg aatcgcccga tcgaattctt ccgcttttag aagaactctc
     2581 gcggctggta gctgaaagaa acgcaaaatg caaatatctg aaatgagcgt ttcgagaagc
     2641 ctcgaaaacc gatgattttc acgagaagaa accgaaagaa ctgacctcgc agcatcactg
     2701 ctcaagaaac ggcaaggagt g
//
LOCUS       DAOPXN010000246         2712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_39061_length_2712_cov_5.111404, whole genome shotgun sequence.
ACCESSION   DAOPXN010000246 DAOPXN010000000
VERSION     DAOPXN010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39061_length_2712_cov_5.111404"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..2522)
                     /locus_tag="PKM49_09770"
     CDS             complement(165..2522)
                     /locus_tag="PKM49_09770"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNY83121.1"
                     /translation="MRVLSRWSLRTQILTVLVVSLIIGMFIFGVLLLRNFEVKVRAAE
                     YKEALTFSKGVTDFLDEKRQTALIGAYTLSQDSAATKLFAEGKRTELMTLLEERYRTL
                     NTQMGVYQLQYHLPPATSFLRLHSPQKYGDDLSALRPAIVKVNKEKTNVSGLEKGTEG
                     YGIRGIVPVFYEGKHIGSLEYGMDFGKTLLEELKTTYNGDFALIEFSKGSEPEKIIAS
                     TFSAQTNAMTAQEQTRVKNGDSVIHLEEKKGERHLFLPVIDFQKKIEAYLLICVPSDY
                     YSSRVAVFWLVFISAGVFVIALLAIFFLLVNRAVRPILKLENRIKALSEGEADFTLRL
                     PEEGTKEAVNLSRYINNFLERMRVSFNRVADTSQKNVNKMIVIERGLETFKENYSNME
                     KQVKVTDTDVQNISASVEEQNAGLEEISSASQQLADTADGLNHTSSAIAKQAQEGQES
                     LDKVTVVMDQLIHTMKDVSESTQQLSEKTSRLNLVVETITSIADQTNLLALNAAIEAA
                     RAGEAGRGFSVVADEIRKLAEESKGASQEITRNLGDILSNFERNAQLISTLSEEAFGM
                     LQTNSQTRTIIVSILKGIDDINHMVANLAGGVQEQGATLEEMSAAAQTISDSSINVAE
                     IMEKVVSEVESVKERSDELESEMKEAISFTEEMLKALSVFRVKRPADYLPIIDQAIHD
                     HERWVENLKKRLKDKEIFIEGNPNKCSFGVFLNLVPAPFGHEEEWRKMYDVHRRLHES
                     GHSVIELIKKGDAKNADQEYQKAYHLSRQVIDILGAIASSLKATT"
BASE COUNT          610 a          674 c          641 g          787 t
ORIGIN      
        1 aagtgtggcg ttcccaagtg cgaatcgcga gacttcgtgg gcttccgaag tcagcccgct
       61 ggttaccccg cgatgaaccg caaagtgcgg cgttcccaag gcgtttccag tagaagagcc
      121 ggacttttgt ccggctcttc taacacattg catcttgctt tcgcttaagt tgtagctttc
      181 aggctggagg cgatggcgcc aagtatatct atgacttgcc gactgaggtg gtaagctttt
      241 tgatattctt gatccgcgtt tttggcgtct ccttttttaa tcaattcgat tacggagtgg
      301 ccgctttcgt ggaggcgacg atgtacgtcg tacatttttc gccattcctc ttcgtgtcca
      361 aatggggcgg gcacgaggtt aaggaacacg ccgaaggagc acttgttggg gttgccctct
      421 ataaaaatct ctttatcttt tagccgtttc tttaaatttt ccacccatcg ttcatgatcg
      481 tggatggctt ggtctatgat cggcaaataa tccgcaggcc ttttcacgcg gaacaccgaa
      541 agggctttca gcatctcttc cgtaaaggag atcgcttcct tcatctccga ttccaattcg
      601 tcggaacgtt ctttcacact ctccacttcg gaaacgactt tctccattat ctcggcgaca
      661 tttatcgaag aatccgagat cgtctgcgcg gctgcagaca tctcttccag agtcgccccc
      721 tgttcctgga cccctccagc caaattcgcg accatatgat tgatatcgtc tatgcctttc
      781 aagatactga caatgatcgt gcgggtttgc gagtttgttt ggagcatacc gaaggcttcc
      841 tcagacagcg tggaaatcaa ttgcgcattt ctttcgaagt tgctcaggat gtcgccgaga
      901 ttacgcgtga tctcttgtga tgctcctttg ctctcttcgg ccaatttccg tatttcgtcg
      961 gccacgactg aaaagcctct gcccgcctct cctgccctcg cggcctctat agccgcgttt
     1021 agcgcgagaa ggttcgtttg atccgcaatg ctggttatgg tttctaccac caggttaagc
     1081 cgagatgttt tctccgaaag ctgctgcgtg ctttccgaga catctttcat cgtgtgaata
     1141 agttggtcca taacgacggt gaccttatcg aggctctcct gtccttcttg cgcttgcttc
     1201 gcgatggcgc ttgacgtatg attaaggccg tcggcggtat cggccagctg ttggcttgcg
     1261 gaggagatct cttcgagacc ggcgttttgt tcttctaccg aggcgctgat gttctggaca
     1321 tccgtatcgg tgactttaac ctgcttttcc atattcgaat agttttcttt aaaggtttcc
     1381 aagccccgtt ctatcacgat cattttgttc acgtttttct gtgaggtgtc ggccacgcgg
     1441 ttgaaagata cgcgcattcg ttctaaaaag ttgttgatgt agcgactgag gtttacggct
     1501 tcctttgttc cctcttcagg aagacgtaag gtgaaatcgg cctcaccttc agaaagcgct
     1561 ttaatgcggt tttccagttt taaaataggt ctgaccgccc ggttgaccag gaggaagaat
     1621 atggccaaga gcgctatgac gaaaacaccg gcactgatga agactagcca aaacacagcc
     1681 acacgcgaag agtagtaatc ggagggcaca cagatcaata aataggcctc gatctttttt
     1741 tggaagtcga taaccggtaa gaacaggtgc ctctctcctt ttttttcttc cagatgtatg
     1801 accgaatccc catttttgac acgtgtttgc tcttgggccg tcatcgcgtt tgtttgcgcc
     1861 gaaaaagtcg aagcgatgat tttttcaggc tcgctgcctt tggagaattc gatgagcgcg
     1921 aaatcgccat tgtacgtggt tttcaattcc tcgagaagag tctttccgaa gtccatgccg
     1981 tactccaacg agccgatgtg ttttccttcg tagaatacgg ggacgattcc gcgtatgccg
     2041 tacccttcgg tgcctttttc caaaccgctg acgttcgttt tttccttatt cactttcacg
     2101 atggcgggtc ttaaggcaga caaatcgtct ccgtacttct gagggctatg taagcgcaaa
     2161 aagctcgttg caggaggaag gtgatattgg agctgataaa cgcccatttg cgtgttgagt
     2221 gttctgtatc gttcctcaag aagcgtcatt agctcggttc gtttcccttc cgcaaacagc
     2281 tttgtcgccg ccgagtcttg agaaagcgtg tatgcgccaa taagcgccgt ttgtcttttt
     2341 tcgtcaagga agtctgttac gcctttagaa aaggtaagcg cttctttgta ctcagccgct
     2401 ctcaccttca cttcgaaatt tcgaagcaag aggactccga aaatgaacat accgatgatt
     2461 agacttacga ctaaaaccgt gagaatctga gttcttaagg accaacgaga caaaacacgc
     2521 acaattgtcc acctccatga tcgggttatc aaatctgaat tataacataa tcagaatgcg
     2581 ttttggaaaa cgaaaactcc gtacttcctt agaaacaaaa ataatagtta actcatcctg
     2641 tgcgtggatg taacagcaaa accaacactc gctgtgaagc ctttgaatac ctgcactcag
     2701 agagaaagga tt
//
LOCUS       DAOPXN010000247         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_39302_length_2701_cov_4.894180, whole genome shotgun sequence.
ACCESSION   DAOPXN010000247 DAOPXN010000000
VERSION     DAOPXN010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39302_length_2701_cov_4.894180"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PKM49_09775"
     CDS             <1..968
                     /locus_tag="PKM49_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83122.1"
                     /translation="LYDKLLSIMSWVEEILFFQKSEPKGKLLAFSGGEPILTTLPPSL
                     FQTPKELWVGQLSLGLPEDARDTVSEGLTPRLKKALTSKAVSIDKRDLILLVLLKSRS
                     YDPSDYEFHFLRTLFNISELYLKIFYSTKRNETISQKYILLCRFQMTLCSPQALESSS
                     LGNLTGGREGTQKIASAFISFLRKIYPEASLWGLRIGPEERPEEQTVYYALKSSRSDP
                     NAGAPFTIEKRRLNEVVKEPFSSSLSPSKPFMGEIAEKEGIAPGSHSRIYLPLYARSE
                     SVVALLMEFSFFKVFSNVEKEYLFFLCQILGEAFDAVHQIGRAPG"
     gene            965..1537
                     /locus_tag="PKM49_09780"
     CDS             965..1537
                     /locus_tag="PKM49_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83123.1"
                     /translation="MRANRVGTHIANTRWTLFLIALLTLCGACLCASEYRTSLHAQFP
                     HRVKELESILGMAVEESFVAVREFSSVASFTRETGAPYWIRGFTNANGICLQSRHLLG
                     ESVYRNLLEHELLHWTIRRLADFPLWFEEGIVCLITGELSGYRGIPVMKNVEAVDPLT
                     LKNPWEMVSYSLGCVETVKEILYKHTEGCP"
     gene            1534..2286
                     /locus_tag="PKM49_09785"
     CDS             1534..2286
                     /locus_tag="PKM49_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83124.1"
                     /translation="MIYPIPTRTYKKKPKRKVPWILILISLACTFVVLFLFYTHSLLF
                     DSNDMKEPIVDFLFLIQDKEEVFFIRSNKLQKISYAVQMPLKSFEPIQAVSLDQSTPQ
                     GIYNSIEKLFGRANRSFWATIDTSDFAALARLSKTAQDSSQTGASQTPHDIHQFIGLI
                     QRIDLSVFEFIFFPKMKALQNILKMDNFNNRSAYRLVDQLKNYAYKATPVTFLTKNPV
                     TIRYQSKSGEVEVERFYLDDKSLKSIKEFMSQ"
     gene            2283..>2701
                     /locus_tag="PKM49_09790"
     CDS             2283..>2701
                     /locus_tag="PKM49_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83125.1"
                     /translation="MRRAHRREGGVLLALFTSLLCVSVTLFFLILPGFMKEVFQVSDE
                     ESALTGKAYYLILGVDDAATGGADRTDMIGVLGIHFETKKTVFISIPRDLMVPDPASE
                     SAPSPTATGSAVQSQPTVKINGLYKKHGIRALQKTIES"
BASE COUNT          657 a          728 c          662 g          654 t
ORIGIN      
        1 agctgtacga taagctgctt tccatcatga gctgggtgga ggagattctc ttcttccaga
       61 agtccgaacc caagggtaag ctcctggcct tctccggagg agagccaatc ttaacgacgt
      121 tgcctccctc tctctttcaa acccctaaag agctctgggt tggccagctt tcgctgggcc
      181 ttcccgagga cgcgcgagac acggtttcgg aaggcttgac gccccgactg aagaaggcgc
      241 taacctccaa agccgtatcg atcgacaaac gcgacctgat cctcctcgtg ctgttgaaaa
      301 gtagatcgta cgacccttct gactacgagt tccatttttt acgcaccttg ttcaatatct
      361 cggaactcta tctaaaaata ttctattcta cgaaacgaaa cgagacgatt tcgcaaaagt
      421 atatcttgct ctgccggttt cagatgaccc tctgttcacc gcaggcgctt gaatcctcgt
      481 cgctggggaa tttaaccggt ggtcgagaag gaactcagaa aattgcctcg gctttcatct
      541 cgtttctaag gaaaatatac ccggaagctt ccttgtgggg gttgaggatc gggcccgaag
      601 agaggccgga agagcaaact gtatactacg ccctcaagtc gtctcgctcc gatcccaacg
      661 cgggcgcccc cttcacaatc gaaaaaaggc gtttgaacga ggtggtcaaa gaaccgttct
      721 cttcttcgct ctcgccatcg aaaccgttta tgggagagat tgccgagaaa gaagggatcg
      781 cgcccggatc ccattctaga atctacttgc ccctttacgc gcgttccgaa tcggttgtcg
      841 cgctgttgat ggagttttct ttcttcaaag tcttttccaa cgtagaaaaa gagtacctgt
      901 tctttttgtg ccagatcctg ggggaagcct tcgacgccgt tcaccagata gggagggcgc
      961 ccggatgagg gccaatcgcg tggggacaca tatcgcaaac actagatgga ccctattcct
     1021 catagcgctg ctaacgctgt gcggggcttg tctatgcgcg agcgaatacc gcacctcttt
     1081 acacgcgcag tttcctcacc gagtgaaaga gctggagtcg atactgggta tggccgtcga
     1141 ggaatccttc gtcgcggtca gggagttctc gagcgtggcc tctttcacac gagagacggg
     1201 cgctccctat tggattcgcg gattcacgaa cgcgaatggg atttgcctgc agagtcgcca
     1261 cctgttggga gaatccgtct atagaaatct tttagaacac gagctacttc attggacgat
     1321 acgtcggcta gccgatttcc ccctctggtt tgaggaaggg atcgtctgcc tgatcaccgg
     1381 agagctctcc ggttatcggg gtatcccggt gatgaaaaat gtggaagcgg ttgacccgct
     1441 aaccttgaaa aacccttggg aaatggtatc ctattcactg ggctgcgtgg aaactgtgaa
     1501 agaaatcctg tataaacata cggaggggtg tccatgatct atccgatacc gacacgcacc
     1561 tacaaaaaaa agccgaaacg caaggttcct tggatcctaa ttctcatctc gcttgcctgc
     1621 acgttcgtag ttctatttct gttctatacc catagtctgc ttttcgatag caacgatatg
     1681 aaggagccga tcgtcgactt ccttttcctg atccaagaca aagaggaggt cttcttcatc
     1741 cgttcgaaca agcttcaaaa aatcagctac gccgtacaga tgcccttaaa aagcttcgaa
     1801 ccgatccaag ctgtctcttt ggaccaatcg acgccgcaag gcatctacaa ttcgatcgag
     1861 aaactcttcg gtcgggctaa tcggagtttc tgggcaacga tagacacctc tgatttcgcc
     1921 gccctcgcgc gactgtccaa aaccgcgcag gactcttcgc aaaccggcgc ttctcaaact
     1981 ccacacgata tccaccaatt catcggattg atccaacgga tcgacctttc cgtcttcgag
     2041 ttcatcttct ttccaaagat gaaggcgcta cagaacatcc tcaaaatgga taatttcaat
     2101 aaccgctcgg cctaccggtt ggttgaccaa ctcaaaaact atgcctacaa agccactccg
     2161 gtgacatttc tgacgaaaaa tccggtcacc atccggtatc aatccaagtc gggtgaggtt
     2221 gaggtggagc gcttctatct agacgacaag agtttgaagt cgattaagga gtttatgtcc
     2281 caatgagaag agctcatcgg cgcgaaggcg gagtgttatt agccctcttt acctctcttc
     2341 tttgcgtatc ggtcacccta ttctttctaa tcctgcccgg ctttatgaag gaggtctttc
     2401 aggtttccga tgaggaaagc gcgctgacgg ggaaggcgta ttatctgatc ttaggggttg
     2461 acgacgcggc cacgggaggg gcagacagaa cggatatgat cggcgttctg ggtatacact
     2521 tcgaaactaa aaagacggtc ttcatcagca tcccgaggga cctgatggta cccgatcccg
     2581 cctcggaatc cgcgccttcc cccactgcca ctgggagcgc cgttcagtcg caacctacgg
     2641 tcaagatcaa cggactatac aaaaaacacg gcatccgcgc cttacaaaaa accatagagt
     2701 c
//
LOCUS       DAOPXN010000248         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_39480_length_2692_cov_4.198711, whole genome shotgun sequence.
ACCESSION   DAOPXN010000248 DAOPXN010000000
VERSION     DAOPXN010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39480_length_2692_cov_4.198711"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PKM49_09795"
     CDS             complement(<1..202)
                     /locus_tag="PKM49_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNY83126.1"
                     /translation="MIQLPELRIGHNVIPLPIVQGGMGVGISLAGLASAVANEGGIGV
                     IASAGIGTFHFDWDQGTMSLMDL"
     gene            897..2150
                     /locus_tag="PKM49_09800"
     CDS             897..2150
                     /locus_tag="PKM49_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HNY83127.1"
                     /translation="MRKTLLTLVLLCVLFTSILFSNTTLRMIQVFASPERTKIIENII
                     EKFEALKPGVKIELISPPYETAVSKVNIMMSNEEPLDIVEVSDWLLSGLVSMGVLENL
                     ERYITRSEQAKYWVDGVVEAARTVNNTAYIMPNAIYVKTLFYRPDILEKYNILGAPRS
                     MGEMMAICQYITNGRDQYGYSYRGIDPVNFMDLVLCSFFNDIDPACMYKTTSGKIIFE
                     DPRALEGLKFYIELYHKASPKDSISWGFDEQVNAFVSGLTPILFQDPDTTALLNYLLT
                     TGVYKTAPLPVGPGGKVYPTFGFGGWGIPSYSKNKDIAWEFIEFFNLPENAAYFCKEY
                     GALPVDSRIYESDPYFGSAVFDGWAAMFDDPVTYQITAYPIDNELWGEWFRLQGDIQE
                     RLLMNRMTPERALQEFTNFWKDAGI"
     gene            2137..>2692
                     /locus_tag="PKM49_09805"
     CDS             2137..>2692
                     /locus_tag="PKM49_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY83128.1"
                     /translation="MPGSKQKKGAQAPFSSPEMRPHHSLKKKSRRFFFLLALFIPTFS
                     VIGFLTIYPTIRGFILSFQNYTVFNIRRIRYIGLQNYERVLQSGDFGLILWNTFVWIF
                     FSVLFQMLLGLGLALLMRKPFKGRGIYAGFVFYPWALSGFAIGLIWRWLFDGSFGIIN
                     DILIRLGFIETGIGFFSDPTLAMLA"
BASE COUNT          667 a          688 c          652 g          685 t
ORIGIN      
        1 ccaggtccat cagggacatc gtgccctgat cccaatcgaa atggaaggtg ccgatccctg
       61 cgctcgcgat gacgccgata ccgccctcat tcgctaccgc gctggccaag cctgccaatg
      121 aaatgccgac gcccataccg ccttgcacaa taggtagtgg gatcacattg tgtcctatac
      181 gcaactccgg taactgaatc atatccaact tcttatcatc tctctttcta ttgaacgaat
      241 tcaaagcttc aaaacaggcc tctcgttttg tggcgattat acacgaaatc ggaccgctca
      301 ggaaatggcc tgcgaatttg gcccttaagc gaggcgattc ttataggtta gacgaaaacg
      361 ggacgcgcgc gggagcgtct ggaaaagcgt aaaaaaagac gtgccggact gaagtccggc
      421 gctctcaggg ttgcgcccga gagcgcttgg aaaagcgaaa tcacaagtat gcccaataaa
      481 cgttcggcgt tcccaggcgt tcacagagag ataggctgac tggaagtcgg ccccacgagt
      541 tttcttcagt cttttctctg tgttctctgt gcgaaccctg tttttcaaga cacgtacaaa
      601 aacgtatcgc gccgtcgcga aaagaagaag cgcttacgcc gcgatgcgcg tgtgtgggtt
      661 atcgcggggt aaccaacggg accccgcgat ccgcctttct tctctttaaa tgcgtatcgc
      721 gccgtcgcga aaagaagagg cgcttacacc gcgatgacta cgttgactaa aaaacgtgcc
      781 atcgactggt cttgtttctt gattgcatct catggcttga cccacaccta tacagaccct
      841 tagggtgttt ttatgatata atggaatcga acataatata gaagggagga gcattaatga
      901 gaaaaacact tcttacactg gtacttttat gcgttttgtt tacttcgatt ctgttctcca
      961 acaccaccct ccggatgatt caggtctttg ctagtcccga aagaaccaaa atcatcgaaa
     1021 acatcatcga aaagtttgaa gccttaaaac ctggcgtcaa gatcgagttg atctcacccc
     1081 catatgaaac ggcggtgtcg aaagtcaaca ttatgatgag taacgaagag cctttggata
     1141 ttgtggaagt aagcgattgg ctgctcagtg ggcttgtgag catgggtgtt ttggaaaatt
     1201 tggaacgata catcactcgc tccgaacaag ccaagtattg ggtggatggg gtagtggaag
     1261 ccgcgagaac ggtgaacaat accgcctata ttatgccgaa cgcgatctac gtaaagaccc
     1321 tcttttaccg accggatatc ttggaaaaat acaacatcct cggcgcccct cgctcgatgg
     1381 gagaaatgat ggccatatgc caatacatca caaacgggag agaccaatac gggtattcct
     1441 atcgcgggat cgacccagtc aactttatgg acctcgtgct ctgctccttc ttcaacgaca
     1501 tcgaccccgc ttgtatgtac aaaacgacct ccggaaagat catcttcgaa gaccccaggg
     1561 cgctcgaagg attgaaattc tatatagaac tgtatcataa ggcctcaccc aaggactcga
     1621 tatcctgggg tttcgatgag caggtgaacg cgttcgtttc gggcctcact cccattctct
     1681 ttcaagaccc ggacaccacc gctctcctga actacctgct gactaccggg gtatacaaaa
     1741 cggcgccttt acctgtggga cccggaggga aagtctatcc gacgttcggg ttcggcggct
     1801 ggggaattcc atcgtattct aagaacaagg acatcgcttg ggaattcatc gaattcttca
     1861 atctgccgga gaacgccgcg tatttctgta aagaatacgg cgccttgccc gtggattccc
     1921 gtatttacga gagtgacccg tatttcggtt ccgcagtgtt tgacggttgg gcagcgatgt
     1981 ttgacgatcc cgtgacctac cagatcactg cataccctat cgacaacgaa ctgtggggcg
     2041 aatggttccg actgcaagga gatatacagg agcggctcct gatgaaccga atgactccgg
     2101 aaagagccct ccaggagttc accaatttct ggaaagatgc cgggatctaa gcaaaaaaaa
     2161 ggcgcgcaag cgcctttttc ttctcccgaa atgcgacctc atcattctct aaaaaagaaa
     2221 agccgacgat tcttctttct gttggctctt tttattccta ccttctcggt catcggattt
     2281 ctcacgatct atccgacgat ccgaggtttc atactgtcct tccaaaacta caccgtcttc
     2341 aatatccggc gaatccgata catcggactt caaaattacg aacgggtttt acagagtggc
     2401 gattttggcc tgattctttg gaacaccttc gtgtggattt tcttctcggt gttgtttcag
     2461 atgctcctgg ggttgggttt ggcgctctta atgcgaaaac ctttcaaagg gcgaggaatc
     2521 tacgcggggt ttgttttcta cccctgggcc ttatccggtt tcgcgatcgg tttgatttgg
     2581 agatggctat tcgacggttc tttcggcatt attaacgata tcttgatccg attaggtttc
     2641 atcgaaaccg gtatcggctt cttttccgac ccaacgctcg cgatgctggc gg
//
LOCUS       DAOPXN010000249         2650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_40350_length_2650_cov_6.275145, whole genome shotgun sequence.
ACCESSION   DAOPXN010000249 DAOPXN010000000
VERSION     DAOPXN010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40350_length_2650_cov_6.275145"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PKM49_09810"
     CDS             complement(<1..516)
                     /locus_tag="PKM49_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNY83129.1"
                     /translation="MESFIAACAQFEILPMKVEENLQNAWNTLKACKDATKAHLIVLP
                     ESFTTGFTPIGGLHALWEVAEPIPGRWSDIGERWAKELDTTLCFPVYERGERKGIVYN
                     SALLIGPNGILGHYRKTHPFPTERLAADGWTTRGETPFCVQTPLGNIGIVICYDGDFP
                     ELARVTAMKGAE"
     gene            complement(530..2200)
                     /locus_tag="PKM49_09815"
     CDS             complement(530..2200)
                     /locus_tag="PKM49_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HNY83130.1"
                     /translation="MDLSSMNFIEKIIDDDLKSGRIQQVVTRFPPEPNGYLHIGHAKA
                     VTLNHGFAKTFGGRFNLRFDDTNPEKEEEEFMRSIIEDVRWLGCEWDELRYASDYFDQ
                     FYEWALELIRAGKAYVDDQSPEEIRLTRGTLTEPGKDSPFRNRTVEENLRLFEAMRAG
                     EKEEESCVLRAKIDMSHPNMNMRDPIMYRILKKSHYRAKDKWCIYPMYDFAHGYEDAI
                     EGVTHSVCSLEFEDHRPLYDWFLDNVSVPHRPHQYEFARLNLSHTVMSKRILRELVRS
                     GIVSGWDDPRMPTLQGLRRRGFTPSAIRRFLSETGLAKSYSIVDYEFLESVIRDEMNA
                     KAQRAMVVLYPLKIVIENYPADRVEWVEAENNPEDPAAGSRQIPFSREVYIEAEDFME
                     DPPKKYFRLSPGAEVRLKYAYYVTCREVVKDASGKIVEVRCVYDPESKGGGTPDGRRV
                     KGTLHWVSAAHAVKAEIRLYNKLFTLRDMNMMEEGKSYRDYVNPESCVVLKEALAEPM
                     LADASQGASYQFLRQGYFCVDPDSRDGKPVFNRTVTLKDSWAKIQSKA"
BASE COUNT          558 a          784 c          658 g          650 t
ORIGIN      
        1 ctctgctcct ttcatcgccg tcacccgtgc caattccggg aagtccccgt cgtaacagat
       61 cacgatcccg atgtttccca atggcgtttg aacgcaaaac ggggtttcac ccctcgtggt
      121 ccagccatct gccgctaggc gctcggtcgg aaaaggatgc gtcttccgat agtgacccaa
      181 tatcccgttc gggccgatga ggagcgcgct gttatacacg atcccctttc gctctccccg
      241 ctcgtacacc ggaaaacaaa gcgtcgtgtc cagctctttc gcccaccgct ctccaatatc
      301 ggaccagcga ccggggatcg gctccgccac ctcccacaag gcgtgcaggc cgccaatcgg
      361 ggtgaagcct gtggtaaagg actccggcag tacgatcaga tgagccttcg tcgcatcttt
      421 acacgctttc agcgtattcc aggcattttg caggttttcc tccaccttca tcggaaggat
      481 ttcgaattgc gcgcaagcgg cgataaagga ttccataggt cactccccct taagccttac
      541 tctgtatttt ggcccagcta tctttcagcg tcactgttcg attgaacacg ggtttgccat
      601 cgcgcgagtc ggggtcgacg cagaaataac cttgacgtag aaactgatag ctcgcgcctt
      661 gtgaggcgtc agccaacatc ggctccgcca gcgcctcctt tagaacgacg caagattctg
      721 ggttcacgta atccctatag cttttccctt cttccatcat attcatatcc cgcagcgtga
      781 agagtttatt gtatagccga atctctgctt tcacggcgtg cgcggccgaa acccaatgga
      841 gtgtcccttt cacgcgtctg ccgtctgggg tgccgccgcc cttcgactcc ggatcgtaca
      901 cgcatcgaac ttctacgatc tttccggaag cgtctttgac aacctcccgg catgtgacgt
      961 aataggcgta cttcaatcgc acctccgcgc cgggagacag acggaagtac ttctttggcg
     1021 gatcttccat aaaatcttcc gcttcgatgt agacttctcg gctgaacgga atctgtcgtg
     1081 aaccggcggc ggggtcttcg gggttgttct ccgcttccac ccattcgacg cgatccgcgg
     1141 gatagttttc tatcacgatt ttcaagggat agagaacgac catcgcgcgt tgcgccttcg
     1201 cgttcatctc gtctcggatc accgactcga ggaactcata gtcgacgata ctatagctct
     1261 tcgccaagcc ggtttcggac agaaacctcc gaatggcgct cggggtaaac cctcggcggc
     1321 gaagcccttg aagcgtcggc attcgggggt cgtcccaacc ggatacgata ccggaccgaa
     1381 ccaattcgcg caagatccgt ttgctcatca ccgtgtggct gaggttaagc ctggcgaatt
     1441 catattgatg cgggcgatgc gggacgctga cgttatccag gaaccaatcg tacagcgggc
     1501 ggtgatcctc gaactccaaa gagcacaccg agtgcgtgac cccttcgatc gcgtcttcgt
     1561 atccatgtgc gaaatcgtac atagggtaga tgcaccactt gtctttcgcg cgatagtggg
     1621 attttttcag gatccgatac ataatagggt ctcgcatatt catattcgga tgcgacatat
     1681 cgattttggc tctcaacaca cagctttcct cttccttctc tcccgcgcgc atagcttcga
     1741 agaggcggag attctcctcg accgttcggt ttcgaaatgg agagtcctta cccggttcgg
     1801 tcagcgtgcc gcgcgtgaga cgtatctctt ccggggattg gtcgtccaca taggctttcc
     1861 ccgcgcgaat cagttcgagc gcccactcat aaaattgatc gaaatagtcg gaggcatagc
     1921 gcaactcgtc ccactcgcag cccaaccaac gaacatcttc tatgatggag cgcatgaact
     1981 cctcttcctc tttctcggga ttcgtatcgt caaaccgcag gttgaaccgg ccgccgaagg
     2041 tcttcgcgaa cccatgattc agagtcaccg ctttcgcgtg cccgatatgc agatagccgt
     2101 tcggctcggg cggaaaccgg gtcaccactt gttgaatacg cccggacttc aaatcgtcat
     2161 ctatgatctt ctctataaaa ttcatactgg ataaatccat aattctcttt cctcccgcga
     2221 gctggaaagc aagacaaaag aaggctttcc gcgtgacttc ttttcgcgat tataacacat
     2281 tttcccaaca caggcggcgc ttatgtgggt caaaatatgg gtgaaatatc taagaggttc
     2341 atcgcggggt aacagcaggc tgacttcgga agcccaagtc tcgcgatccc ccttttgttt
     2401 tttgcaccgc gaggatatcg gggtatcgcg ccttggggga atcgcggggt aaccgaaggg
     2461 accccgcgat ccccctttta ctcttatgat ccggcctggg aacgccacac tttagtgtgg
     2521 catgggttca ttgttcactc cgtgccctct gtgccctctg tgcgaaccct gttttctggg
     2581 agcgccggac tttaacccca ctttcgacta aaagagctac aaacggcgat ataagccact
     2641 ttctcaaaaa
//
LOCUS       DAOPXN010000250         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_40898_length_2624_cov_5.371740, whole genome shotgun sequence.
ACCESSION   DAOPXN010000250 DAOPXN010000000
VERSION     DAOPXN010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40898_length_2624_cov_5.371740"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="PKM49_09820"
     CDS             <1..384
                     /locus_tag="PKM49_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HNY83131.1"
                     /translation="NEAYVRITTVFVTREETERSERLYGTFIHYIYYLTAREDFSRLV
                     QLTRTVSVERSERMQTIAEWLIQDGMEKGREQLLWKQISKKFPQVPKAYYEKLKTLTI
                     DQLDNLGLELMDMQNEEELKKHLPM"
     gene            complement(789..1511)
                     /locus_tag="PKM49_09825"
     CDS             complement(789..1511)
                     /locus_tag="PKM49_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83132.1"
                     /translation="MIVSIGAYYGYRNRKKAMDVAAPLLDRASRIIAGGFFPLFIAAR
                     FQNEHDFPDTFLGSFRKELKALENDARFPLRVWSCYRLMRRLSIIEDLGARIAKAILE
                     AGVSEEALHRPLTHPLWVELYRLDTGAVYVQAVRSLYDSITVSMLHPGIAVSQEGVFN
                     TMYLTPFPCLSLLEESGQTVTFLSQSYVYGYTKPIVRYLKTLQFIRSEDLCRKAVDRI
                     SEKNTVTEIDLQTGSSPGFYRK"
     gene            complement(1508..2332)
                     /locus_tag="PKM49_09830"
     CDS             complement(1508..2332)
                     /locus_tag="PKM49_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNY83133.1"
                     /translation="MIVKDTVRALVLSGGGAKGAVHLGALMELEGMNLQWDLIVGTSI
                     GAMVAAGYALTCNTETMRRYAEGMNRTVRVNVNRPKRIPPLLACWFTNTFQSLFPRSF
                     FFKVVERVFGDRTFEDTRIPLLVTACDIEKRRLTVFREGPLITPLLASMAIPGVFPSV
                     RVGTSLFCDGGVLENLPIPTALTAGANRILALHICSKPDEEGGPIASSSTGLLLQTDA
                     FRERWQERLTDKALAKVIDFDTTGYGSMDFRHTPALLEIGRKTIRRRREEIREWLS"
BASE COUNT          653 a          715 c          686 g          570 t
ORIGIN      
        1 aatgaagcgt acgtaagaat caccaccgtc ttcgtcacgc gggaagaaac ggaaagaagc
       61 gaacggcttt acgggacttt tattcactac atctactatt tgacagcgcg agaggatttc
      121 tcgagactgg tacaactgac gcgaacggta tcggtggaaa ggagtgaaag aatgcaaacg
      181 atagcggaat ggcttattca agatgggatg gagaaaggac gtgagcaact cttatggaag
      241 cagatctcca aaaaattccc tcaagtcccg aaggcgtact acgaaaagct gaaaacgctc
      301 acgatagacc agctcgacaa cctgggttta gaactgatgg atatgcaaaa cgaggaagaa
      361 ctgaaaaaac atctgccgat gtgaaaggtt tcggcgtctc attaggagcg cctgagagcg
      421 cctatctttt gttcggcggg aggctgtttt tgcctttcag agaacgccac gtgcacgccg
      481 gactttagtc ccgcaagtta tagcttttct gggaacgccg aacttcagtt cggcatatcc
      541 caaaaggagt aatactgcag agcatatcgc ggagtcgtaa aaatcactta ccccgcgata
      601 acctccgaaa acgactttca aaggattttc ctcttaccgt tctagctaac ccccttttcc
      661 ttctattctc cgtgttctct gtgttctctg tgtgaacctt cttttcccct tttgtattaa
      721 accctgccgg actaaagttc ggcgctctca gtagcgatcc cgtaagagac gactccaagg
      781 aatgtgcgct attttctata gaaacccggt gacgagcccg tttgaaggtc tatttccgtg
      841 accgtgttct tttctgagat acggtcgacg gcctttctac ataggtcctc acttctgata
      901 aactgaaggg ttttcagata acggacgatc ggtttggtat acccatagac ataggattgc
      961 gacaggaaag tcacggtttg gccggattct tccagcaaag acaggcacgg gaaaggtgtg
     1021 aggtacatcg tattgaaaac gccttcttgc gaaacggcta tccccggatg aagcatcgaa
     1081 acggttatcg agtcatacaa cgaccgaacg gcctggacgt agaccgcgcc cgtgtcgagc
     1141 cggtacagtt cgacccacag aggatgcgtt aagggacgat gcagcgcctc ttccgagact
     1201 ccggcctcca ggatggcttt cgctatccga gcgcccaaat cctcgataat cgataagcgc
     1261 ctcatcaggc gataacaact ccatacccgc aggggaaacc gggcgtcgtt ttccaacgct
     1321 ttgagctctt tgcgaaagga ccccaaaaac gtgtcgggga aatcgtgttc gttctgaaac
     1381 cgtgcggcga tgaacaacgg gaaaaaaccg cctgcgatga ttcgagaagc gcgatccaac
     1441 aggggagcgg ccacgtccat agccttcttg cggttccggt acccatagta ggccccgatc
     1501 gagacgatca agacaaccac tcccggattt cttcccgccg gcgacgaatc gttttcctgc
     1561 ctatttcgag caaagccggg gtgtggcgaa aatccataga accgtacccc gtcgtgtcaa
     1621 aatcgatgac cttcgcgagt gccttatccg tcaatcgttc ttgccagcgt tctcgaaagg
     1681 cgtccgtttg aaggagcaat cccgtggagc tggatgcaat cgggccgcct tcttcgtcag
     1741 gtttcgagca gatatggagg gcgaggatcc ggttcgcccc ggccgtaagg gcagtcggta
     1801 tcggaaggtt ttccaagacg cccccatcac agaacaagct cgttccgacg cgaaccgaag
     1861 gaaagacccc cgggatagcc atactggcca gcaagggtgt gatcaggggg ccttcgcgaa
     1921 agacggtcaa gcggcgtttc tcgatatcgc aagccgttac gagtaacgga attcgggtat
     1981 cctcaaaggt tcgatctccg aagacccgtt cgacgacctt aaaaaagaac gaacgcggaa
     2041 aaaggctttg aaaggtattg gtgaaccaac aggctaacag aggaggtatc cgttttggac
     2101 ggttcacgtt tacgcgaacc gtacggttca taccttccgc gtagcgccgc atcgtctccg
     2161 tattgcacgt caacgcgtac ccagctgcga ccatcgcccc gatgctcgtt cccacgatca
     2221 ggtcccattg cagattcata ccctccagct ccatgagggc cccaagatgg actgcgccct
     2281 tcgcgccgcc tcccgacaag accagcgccc tcaccgtgtc tttcacgatc attcgcacct
     2341 ccgcgtaccg ttgtgccctt ttgtctcatt ttaccatagg acgccgcggt cgggttgaat
     2401 gcgcaaaaaa cgggttcgca cggaccacac ggaggacgcg gaggtaagtt tttttgagaa
     2461 cgccgttgaa agccaaagac acgccagact gaagtccgcc gctctggcgg cgccccgaag
     2521 gcgttcccag aaacgtgaag aaaagaaaaa ccccccttac ggagggtttt tggtcggggc
     2581 gactggattc gaaccagcgg ccttcaacgc cccatgctga cgcg
//
LOCUS       DAOPXN010000251         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_41204_length_2611_cov_5.492567, whole genome shotgun sequence.
ACCESSION   DAOPXN010000251 DAOPXN010000000
VERSION     DAOPXN010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41204_length_2611_cov_5.492567"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1672
                     /locus_tag="PKM49_09835"
     CDS             188..1672
                     /locus_tag="PKM49_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNY83134.1"
                     /translation="MEKKARAVNTKRVYLVRLSCLVGIGCLLMLFSSCQYINRSLEQG
                     VEIEVLREMLIRYSEKMDTMSARIDRLEEQLLQTRRSADALQERVDKNYLLLLTAQSS
                     LTYWEYLNTPPDFSALLQISKTIPDMAAIEEMVRERIAAEMARFRMETSSSDRKETTI
                     QDTVPQASFQERELLLQSELNQLKEDYRQLRWLNYRDNPKLTDPFSLDDLSLVLGGKI
                     EYTIQSGDTLSHIAQAFGLGAEGVKTLMAENKIQDARTIRSGSIIRIPLPPLQERITV
                     PLAGRNRLAPEDILRFFGESTDSGISRGLSFRVWQNQTIVSCLPGRVIDVGATYIVIY
                     HGNGLKGIYNRLEELTVNKGDWVMSGQALGVCRPPEFRLEIMINFEYRDPMLLFLQNM
                     GDFQVTFYTEWDDGNLPFFPYFRRTKEGSFAKEWYSVAADPALLPPGTMVYLPQLRST
                     PSRGLFVVEDVGSAIRSKKLDVYIRDIREAAKLKMTTGVYRFGH"
     gene            complement(1730..2002)
                     /gene="rpsO"
                     /locus_tag="PKM49_09840"
     CDS             complement(1730..2002)
                     /gene="rpsO"
                     /locus_tag="PKM49_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNY83135.1"
                     /translation="MMALEYEEKQNIISDFRINDKDTGSIEVQVALLSTRIRQLTEHL
                     KTHPKDYHSRHGLMKLVGKRRRFLNYLKKNRFDVYRQMIEKLGIRR"
     gene            complement(2130..>2611)
                     /locus_tag="PKM49_09845"
     CDS             complement(2130..>2611)
                     /locus_tag="PKM49_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83136.1"
                     /translation="PVTGSPSKEGGALPAPLPAPTVAPTLQKPSEKKEMKRPVDKRPQ
                     NAHRSSSKAIEAKPVEQKSAFYGIEIGEPRNTTDWLEEDIGRYHRAVIAALSKSPVSP
                     EGILDLSEIWISTSLPLDLIIEVIQTQGLEGNLQEVRSIVFQGRKIWPLPKQVGKED"
BASE COUNT          581 a          682 c          722 g          626 t
ORIGIN      
        1 cgatggctgg aaaggctcaa ccaggagtac aagaactcca attcgcctta cctgacggca
       61 ggtaaggcat tatgggcgtc attccgcgag aaaaagacct aaacgggctt tttagagcgg
      121 agctcgaaga ctatcgtcgg tgagttgcgg gatggagcct ccggggtcgc aaaggagaat
      181 gcggcgtatg gaaaaaaaag cgcgtgctgt caatacgaaa agggtctatc tggttcgtct
      241 atcctgtttg gtggggatcg ggtgccttct gatgttgttc agctcttgtc agtacatcaa
      301 tcgctctttg gaacaggggg tggagataga ggtattgcgc gagatgctga ttcgctatag
      361 cgaaaaaatg gacacgatga gcgcgcgcat cgatcggctc gaggagcagc tcctacaaac
      421 cagacgcagt gcggatgcgc tacaagagag agtggataaa aattacctct tgttgctcac
      481 ggcccaatcg agcttaacct actgggaata cctgaacacc ccgcccgact tttctgcgct
      541 gctacagata tcgaaaacca tcccggatat ggcggctatc gaggagatgg ttcgcgagcg
      601 aatcgccgct gaaatggccc gtttcaggat ggagacctct tcctcggacc ggaaagaaac
      661 gaccatacaa gacaccgtgc cacaggcctc ttttcaggaa agagagcttc tcctccaatc
      721 ggagttgaac cagctgaaag aggactatcg gcagttgcgg tggctgaact accgggacaa
      781 cccaaaactc accgacccct tctcgctcga tgacctatcg ttggtgctcg gcggaaagat
      841 cgaatatacc atacagtcgg gcgacaccct cagccatatc gcgcaagcct tcggcttggg
      901 agcggagggc gtgaaaacgt tgatggccga aaacaagata caagacgcgc gtacgattcg
      961 gagcgggagc ataattcgca ttccgcttcc ccccctacag gaaaggatta cggtgccttt
     1021 ggcaggtcgg aaccggttgg cgccggagga tattctccgg tttttcggag agtcgacgga
     1081 ttcgggcatt tctcgagggc tttcctttcg ggtgtggcag aaccaaacca tcgtctcttg
     1141 tttaccgggc cgtgtcatcg atgtgggcgc cacctatatc gtaatctacc acggcaacgg
     1201 attgaagggg atttataacc gccttgagga gctgaccgtc aacaaaggcg attgggtgat
     1261 gagcggtcaa gccttgggtg tgtgtcgccc cccggaattt cgattggaaa ttatgatcaa
     1321 tttcgagtac cgcgatccta tgctcctctt tctgcaaaat atgggggact tccaagtgac
     1381 cttctacacc gagtgggacg atggcaacct tccgttcttt ccctatttcc ggcgaacgaa
     1441 agaaggtagt ttcgccaaag aatggtactc cgtggcggcc gatcctgcgc tccttccccc
     1501 cggaacgatg gtctaccttc cccagctccg ttccaccccc tctcgcggcc tattcgttgt
     1561 ggaagacgtg ggcagcgcga taagaagcaa gaaattggac gtgtacatcc gagatatccg
     1621 ggaggccgcg aagctgaaga tgaccaccgg cgtctatcgc ttcgggcatt gagcctctcg
     1681 ataggagacg cgggttgtcg cgaaaaaagg cggatttctc cgccttttgt caccttctga
     1741 taccgagctt ttctatcatc tgccggtaca cgtcgaaccg gtttttctta aggtagttca
     1801 aaaaccggcg tcgtttgccg accagcttca ttaacccgtg acgagagtgg tagtccttcg
     1861 ggtgggtctt caggtgttcg gtcaactgac gaatcctcgt cgacaacagg gcgacctgca
     1921 cttcgataga ccccgtgtct ttgtcgttga tacgaaaatc cgagatgatg ttttgctttt
     1981 cctcgtactc tagtgccatc aaaaaatcct cctatctgca acgggacgtt gcgtctatct
     2041 cccctatccc agaaacgggt ttggccttcc cgaatccgag gtagggagcg ctttggaacg
     2101 ccgtatttta tcacatcgta gaagggctgt caatcctctt ttccaacctg tttcggcaaa
     2161 ggccatatct ttcggccttg aaaaacgatg gatctcacct cttggaggtt accttccagt
     2221 ccctgcgttt ggatcacctc tatgatcaag tccaaaggga gagaggtgga aatccatatt
     2281 tccgacaggt ccaagatgcc ttccggagat accggcgatt tacttaacgc ggcgatgacg
     2341 gcgcgatgat atcgtccgat atcctcttcg agccaatctg ttgtattcct cggttctccg
     2401 atttcgatgc cgtagaaggc gcttttctgc tccacgggtt tggcctcaat ggcttttgag
     2461 gagctgcgat gagcgttctg tggccgtttg tcgacaggtc tcttcatttc ctttttctct
     2521 gaaggcttct ggagcgtcgg cgcgacggtg ggtgcgggca ggggagcggg aagcgccccg
     2581 ccctctttgg aagggctccc cgtaacgggt g
//
LOCUS       DAOPXN010000252         2598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_41490_length_2598_cov_5.175383, whole genome shotgun sequence.
ACCESSION   DAOPXN010000252 DAOPXN010000000
VERSION     DAOPXN010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41490_length_2598_cov_5.175383"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..733)
                     /locus_tag="PKM49_09850"
     CDS             complement(239..733)
                     /locus_tag="PKM49_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83137.1"
                     /translation="MKRVLLTVFFIAAWLCAVTAYADGFGIGVGAGQPSGLNFRYEMT
                     YEMSLQGGAGYAFLWNPKGVYLNLDLLYFFRRLIPIGKGSYLPIYVGGGLCEVLWLEE
                     EEGKKQLMTLSALELPIGLYYPIPLNGRNHVEIYGQVRPAIGLFGENYRFLISVEIGA
                     RFHF"
     gene            complement(730..2334)
                     /locus_tag="PKM49_09855"
     CDS             complement(730..2334)
                     /locus_tag="PKM49_09855"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNY83138.1"
                     /translation="MAKKYIVVTGGVLSGIGKGILSASTGRLLKELGIDVLGVKIDPY
                     LNVDAGTMNPNQHGEVFVTEDGYEADLDLGHYERFIGLNMRKENNITAGQIYSSVIAK
                     ERGGKYLGSTVQIVPHVTGEVKERLNQIEAEVVIIEIGGTVGDIEGEVFLETIREMVL
                     EKGKENFLFIHITYVPYLKVTNEYKTKPTQQSVQLLRRIGIQPDVIAARSELPIESPE
                     IRKIALFAGIKPEMVINLPDNKNVYEVPRNLYNMRFHTIILDRLQLTASRDFQWDYPT
                     VFKPLKIGIVGKYLGTDDAYKSIIESIFLCGVEKPTLLDAQELEEMNEEEVHLLLKDY
                     GGLIIPGGFGKRGIEGKLKAIGYARERRKPLLGICLGMQLMILEFARNQLGMKEADST
                     EFNPETERPLIDLMEEQKKTLQMGGTMRLGAQRAPIFKNTRLYEAYGDLEEVFERHRH
                     RYEVNWELCRSFLKLPEEANEENRSKRLIVGSKNEFVESVELEDHPFFIGVQYHPEFL
                     SKVGHPHPLFLSFIRAINGVSKGGSL"
BASE COUNT          582 a          731 c          620 g          665 t
ORIGIN      
        1 gcgatgacta cgtggaccaa aaatcgcgct atcgattctg gccttgtttc ttgattacac
       61 tccataactt gacacatccc cacagatttg tagcgcgaat ccgtcaacat ctgctgactg
      121 atacacggca agaagtcgcc aacaatctcc atcggatttc cggcatatat ccgtcgccca
      181 agtccgacta tggatttaac cggaaaaccc cgggagacct tgcctaaaag acggtggttt
      241 aaaagtgaaa gcgcgcgccg atttcgaccg atattaaaaa acggtaattt tctccgaaaa
      301 ggccaatggc gggacgaacc tgtccgtaga tctcgacgtg attcctgccg tttagcggaa
      361 tcgggtaata caagccgatg ggtaattcga gcgcagacaa agtcatgagc tgtttctttc
      421 cctcttcctc ctctaaccac aacacttcac acaatccacc gcccacgtag atgggcaagt
      481 agctgccctt ccctatcggt atcagtcgtc taaaaaaata gagcaggtca aggttcaggt
      541 aaaccccttt ggggttccac aggaaggcgt agccggcgcc cccttggaga gacatctcat
      601 aggtcatttc gtagcgaaag tttaatcccg aaggctgtcc cgcaccaacc ccgattccaa
      661 aaccatcggc gtaggccgtg accgcgcaca gccatgcggc gataaaaaaa accgtaagca
      721 gcactctttt cacaatgacc cccctttaga tactccattg atagcccgta taaaagatag
      781 gaatagcggg tgcggatgcc ccactttgga taggaactcc gggtgatact gcactccgat
      841 gaaaaacgga tggtcttcca attcgacgga ttccacaaat tcgttcttcg atcccacgat
      901 caaccgcttt gagcggtttt cttcattcgc ctcttccggc aacttcagaa aggaccggca
      961 aagctcccag ttcacctcat accggtggcg gtggcgctca aagacctctt ccaagtcacc
     1021 gtaggcctcg tataaccgcg tgttcttgaa gatcggcgct cgctgtgccc ccaggcgcat
     1081 cgtgccaccc atctgcaaag tcttcttctg ctcttccatc aaatcgatga gcggacgctc
     1141 ggtttccggg ttaaactccg tggaatcggc ctctttcatc ccgagctggt ttcgagcgaa
     1201 ttccaaaatc atcagctgca taccgagaca gattccgagg aggggttttc tgcgttcgcg
     1261 cgcatagccg atcgccttca gttttccctc gatgccgcgc tttccgaatc cccccgggat
     1321 gatcagtcct ccataatcct tgaggaggag gtgtacctcc tcttcgttca tctcctccaa
     1381 ttcctgcgca tccagcaggg ttggtttctc taccccgcac aagaagatcg attcgatgat
     1441 gctcttgtaa gcgtcgtccg tcccgaggta ttttccgaca atgccgatct tcagtggttt
     1501 gaagaccgtc ggatagtccc attggaagtc ccgggaagcg gtcaattgga gacgatccag
     1561 gatgatcgtg tgaaaccgca tattgtaaag gtttcgcgga acctcgtaga cgtttttgtt
     1621 atccggtagg ttgatcacca tctccggttt gatgccggcg aacagagcga tcttccgtat
     1681 ttctggcgat tcgattggta gttcgctcct ggccgcgatg acgtccggtt ggatcccgat
     1741 ccttctcagg agttgtaccg attgttgggt gggcttcgtt ttgtactcgt tcgtgacttt
     1801 aaggtaggga acataggtga tatggatgaa aaggaagttt tcctttcctt tctccaggac
     1861 catttcccgt atcgtttcga ggaagacctc tccttcgatg tcgcccaccg tgccgccgat
     1921 ctctatgatc acgacttcgg cctcgatctg attcagcctt tctttgactt cgccggtgac
     1981 atgcgggacg atctgaacgg tggacccgag gtacttcccc ccccgctctt tggcgatcac
     2041 cgaggagtag atctggcctg ctgtgatgtt gttctctttt cgcatattaa ggccgataaa
     2101 ccgttcgtag tgcccaaggt ctaggtccgc ttcatacccg tcctcggtga caaaaacctc
     2161 tccgtgctgg ttcgggttca tcgtcccagc gtccacgttc aagtatggat cgatttttac
     2221 tcccagcaca tcgatcccca gctctttgag tagacgaccg gtggatgccg acagtatccc
     2281 tttcccgata ccgcttaata cgccgcccgt cacgacgata tacttcttcg ccacgcagtt
     2341 caacccccct cacaatacaa ggattatagc ataaagcaac cttttccagg gtttggtgtg
     2401 ggtcaagtta tggggtgaca taactaagag gttcatcgcg gggtaagtga tttttacgac
     2461 cctgcgatct acactttgcc ctttctacgc cgcaatgggc ttgtacaaag gcgtatcgcg
     2521 ccgtcacgaa aagaaagtgc gcttacgccg cgatgactac gtggactaaa aatcgtgcga
     2581 tcgattctgg ccttgttt
//
LOCUS       DAOPXN010000253         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_42448_length_2562_cov_4.600718, whole genome shotgun sequence.
ACCESSION   DAOPXN010000253 DAOPXN010000000
VERSION     DAOPXN010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42448_length_2562_cov_4.600718"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1837)
                     /locus_tag="PKM49_09860"
     CDS             complement(<1..1837)
                     /locus_tag="PKM49_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83139.1"
                     /translation="MKKSIFRLSIVIVILALMLAGCILKPKTCDIQATVYGPDGLPAS
                     FASVKVGNTVLKADANGTVRAKVTSGKVAFSALEAGSQLISKEVTIDSNRSIRLELDQ
                     RANTYGFIYRKVDGRLKAIYVIPQALSPKAAVFEGKLGLQAEEAGKGHFLFTQEIGDK
                     TRIEWSPLLEGESKHGLYYAFSIPVEGEIEGQVRIYQGTDRSVGDLPEIPLTDEERAI
                     HPDSVLAAVGDVVAPFGRVDVQDLVDFLTNWKGAFQAKYDLGSISTMDFDRPNATATP
                     KPFNASGLVKDSAVNVYDLLVLLNAYKLATSEIAGFTNINKPFPVTNVNAVVAGSGYN
                     VTWSIQHVNDVQESFDVYVGDTSGALTTLVGTATPKTATSYTIPNSAHAGKYVSVVAK
                     NGAFSSEKAAGPALPPNTVTVNVTFETLHSFIVSFSEAYGSLSPANFALSIGTVTGVT
                     PINATQARVMLSGLSYGITVTITVSGVSTSSGKAIPTHTQNVTIPGVSQFYELVLVCD
                     ATGNAIPSDGLSSTMLTATLYDKATGLTLPITAQVQFTATLGSLSQANVGLSGAAAST
                     QLRSVSSLVELTSYVKATIISSTERPELVGLESNTLVVFFVPGGTL"
BASE COUNT          572 a          694 c          682 g          614 t
ORIGIN      
        1 ggagcgtccc acccggtacg aagaacacga ccaaagtgtt ggattccaac ccgactagct
       61 ccggtctctc ggtggaactg attatcgtcg ctttgacgta tgaagtgagt tctaccaacg
      121 atgataccga gcgcaactgc gtcgaagccg ctgctccgga aagtcccacg ttcgcctggc
      181 tcaaggaacc taacgtcgcc gtgaactgaa cttgggccgt tatcggcaaa gtaagccctg
      241 tcgctttgtc gtagagcgtg gccgtcaaca tcgttgagct cagcccgtcc gaaggaatgg
      301 cgttgcccgt cgcatcgcat actaaaacca gttcataaaa ttgcgatacc cccgggatgg
      361 tcacgttttg cgtatgcgtc ggtatggcct ttccggagct tgtggaaaca ccgcttacgg
      421 taatcgttac cgtaattccg taagagagtc cggacaacat tacccgcgct tgtgtggcgt
      481 tgataggcgt gacccccgtc accgtgccga tcgataaggc gaaattggcg ggggaaaggc
      541 tgccgtatgc ttctgaaaag ctgacgatga aactgtgaag cgtttcaaag gtaacgttca
      601 ccgtcaccgt gtttggaggt aaggccggtc cggctgcctt ttcggaagaa aacgctccgt
      661 tcttcgccac cacgctcacg tatttgcccg cgtgcgccga attggggatc gtgtaggatg
      721 tcgctgtttt cggcgtcgct gtcccgacca gtgtcgtcaa cgcgcccgac gtgtctccaa
      781 catatacgtc gaatgattcc tgcacgtcgt tgacgtgctg tatcgaccac gtcacgttgt
      841 agcccgatcc cgcaacaacc gcgttcacat tcgtgacggg gaagggtttg ttgatgttgg
      901 tgaagccggc gatttctgag gtcgccaatt tgtatgcgtt gagcagcacc agaagatcat
      961 agacgtttac tgccgagtct tttaccagtc ccgacgcgtt gaagggcttc ggcgtcgcgg
     1021 tggcgttggg cctgtcaaag tccatcgtcg atatggaacc taaatcgtac ttcgcttgaa
     1081 aggccccttt ccagttagtc aggaaatcca caaggtcttg tacgtccact cgcccgaaag
     1141 gcgcgacaac gtccccaacc gcggccaaga ccgaatcagg gtgtatcgcg cgctcttcgt
     1201 cggttaacgg gatttccgga aggtcgccca ccgagcggtc cgtcccttga tatatcctaa
     1261 cctggccttc gatctccccc tctaccggaa tcgaaaacgc gtagtataac ccgtgcttcg
     1321 attcgccttc gagaagcggc gaccattcta tacgcgtttt gtctcctatc tcctgcgtaa
     1381 agagaaagtg cccctttccg gcttcttccg cctgcaagcc cagtttcccc tcgaagaccg
     1441 ccgctttcgg ggataacgct tggggtatca cgtagatcgc cttcaggcgc ccatcgactt
     1501 tgcgatagat gaacccgtat gtgttcgccc tttggtccag ctccaaccgt atcgagcgat
     1561 tcgagtcgat cgtcacttct ttggagatca actggcttcc agcttccaaa gcgctgaacg
     1621 cgactttccc ggacgttacc ttcgcgcgga ccgtaccgtt tgcatccgcc ttaagtacgg
     1681 tgtttcccac tttgacgctc gcgaacgatg ccggtaaacc gtccggtccg tagaccgtcg
     1741 cctggatgtc acaggttttc ggcttcagta tacatccggc gagcattaag gcgagaatca
     1801 ctatgacgat cgatagtcgg aatatactct tcttcataac taaaccccct ttttgtattt
     1861 tttattctct aaaaattcaa tgtttggttc aatgaggaaa ttgggatttt ccgcacgaat
     1921 cgattatagc ttgtattgag agatattgtc aaatgttttg ggagaataaa actgacaacg
     1981 cagagaaaag atccctggaa accctggggt ggcgccgact tccaagtcgg cgtgggttct
     2041 gggaacgttg actttgaagt tggcctgtct tgaagaaaca gagttcacac agagaccacg
     2101 gagggaggtt tgctgcgaac aacaactttt aaggtggcat gttttaaaaa tagggttcgc
     2161 acagagagca cagagtacac gaaggaaggc ttgctgggag cacttggtaa aaaccaaaaa
     2221 catgccgaac taaagttcgg cgttcccaag gggttccctt ggaggtccga gaaagcatac
     2281 cgaactaaag ttcggcgttc ccaaggggtt cccttggagg tccgagaaag catgccgaac
     2341 taaagttcag cgttcccaag gggttcccga ggatagcggt accgccaccc cgtcgttccg
     2401 gaactccgtg agaaccactg accccgtttt cgtagaggcg aacgtcaccg tcgcgatggt
     2461 caaatcgctt ccgcttgatc gcggccccca aagtcaggag ggtatggata ccgtgaagga
     2521 agcggtcgag ctttcggcgg tctcataagc gtttttcccg ac
//
LOCUS       DAOPXN010000254         2554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_42635_length_2554_cov_4.209684, whole genome shotgun sequence.
ACCESSION   DAOPXN010000254 DAOPXN010000000
VERSION     DAOPXN010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42635_length_2554_cov_4.209684"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PKM49_09865"
     CDS             complement(<1..587)
                     /locus_tag="PKM49_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83140.1"
                     /translation="MNEKRVNLVTAVLLILLLFLLVRGLSLSISRPEPGSLPVKNIPI
                     FPQRGLVLDSGGAPLVINAHKYFYYLDVKYFSDNLARGKVQADLFFEQVYTLFQKTRP
                     QLEEKMRTMAFVPLGVSESAVLEIPRPAALYISIDHRDERSVLYPELVQIIGKVDSYG
                     SGVNGLERAFDSTLASRDVGQIYYERFNNYARLGE"
     gene            complement(601..957)
                     /locus_tag="PKM49_09870"
     CDS             complement(601..957)
                     /locus_tag="PKM49_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83141.1"
                     /translation="MNQDYMSSFPEKPAASPTPSANHTSAIYLEQVKELSRERFFEVV
                     SVAAKILLMTVVIFAAFFVPFFVGMQTNQLNNRASEMKIEIQSMNTKIQEYNQKITAF
                     GDMLLIKNKVQKSEVE"
     gene            complement(1028..1990)
                     /gene="rsmH"
                     /locus_tag="PKM49_09875"
     CDS             complement(1028..1990)
                     /gene="rsmH"
                     /locus_tag="PKM49_09875"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNY83142.1"
                     /translation="MRDETGRVGSDVVDGHPVEGSPPEFHIPVMVQEVVEGLITDPSG
                     IYVDCTFGEGGHTLEILKRIAGHGGKVIGIDKDLEILRYAEKRLKPFEGSFELFHGPF
                     SNVDLILRGLKVRAAHGVLFDLGVSSYQLDAEQRGFSFDKEGPLDMRMDLQSPRKAED
                     AVNGLKEAELEKILRAYGEEPFSRSIAKQIVLRRPILSTLELVEAVRAGIPVKVRQKM
                     RKHFATRTFQAFRIHVNRELEELENGLAKSLQILCQGGRIVTVSFHSLEDRIVKNFFR
                     DHSTKEGSGSGLKIITKKPLVPSEEEVYRNRRARSAKLRIAETL"
     gene            complement(1987..>2554)
                     /locus_tag="PKM49_09880"
     CDS             complement(1987..>2554)
                     /locus_tag="PKM49_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83143.1"
                     /translation="LLKEILIRYNPLILAFENFGYPSPVYGYNPYAMKEFEIAKNHSY
                     ARPYTMDDFRADALTSLLIRFNALRDGLGVRTRFLLLTETNPDRGLREHFQDWVLWLN
                     SGYFGNVALWYWFMDVKTVRYDTLQAFDILANDGFIVGFSPLSLIRAQLDLVLQEILT
                     HPVGGIMIDTFSPEALTLLNLNGIGLPR"
BASE COUNT          604 a          686 c          651 g          613 t
ORIGIN      
        1 atctctccga gtctggcata gttgttgaac ctttcgtagt aaatctggcc gacgtcacga
       61 gaagccaatg tggagtcgaa ggcacgttcc aaaccgttga ccccgctacc gtacgagtcc
      121 acctttccga tgatctggac cagctcggga taaagaaccg accgctcgtc tcggtgatcg
      181 atcgatatat acaacgccgc gggtcgggga atctccaaga ccgccgattc cgagacgccc
      241 agcgggacga acgccatcgt ccgcatcttt tcttccaact gcgggcgggt tttctggaag
      301 agtgtgtaca cctgctcgaa gaaaaggtcg gcttgcacct tcccccgcgc gaggttatcc
      361 gagaagtact tgacgtccag gtagtagaag tatttgtggg cgttgatcac gagaggggcg
      421 ccgccactgt ccagaacgag ccctctttgt ggaaagatcg ggatgttctt gaccgggaga
      481 ctgccgggtt cgggtcggga tatcgaaagc gatagcccgc gaaccaaaag gaatagcaag
      541 agtatcagga gaacggcggt tacgagattg acgcgtttct cgttcaacgg gatcgagtcg
      601 ctattcgact tccgacttct gaactttgtt tttgatcaac agcatatccc cgaaggccgt
      661 gatcttctga ttgtattcct gaattttcgt gttcatcgat tgtatctcga ttttcatctc
      721 cgaggcgcga ttgttcagct ggttcgtctg catcccgacg aagaacggga caaaaaaggc
      781 ggcgaatatc acgacggtca tcaggagtat cttcgcggcc accgagacga cctcgaaaaa
      841 acgttctctg gaaagctctt taacctgttc tagataaatg gccgaggtat gattcgcaga
      901 aggcgtaggt gatgcggccg gtttttctgg aaacgaagac atataatcct gattcaaaag
      961 aattcccccc taaaggaagc ctttctcttc ctcccacgta atcccctaaa agaatcgaaa
     1021 aaaagaatta taatgtttct gctattctga gtttggcact tctagccctg cgatttctgt
     1081 agacctcttc ttcgctcgga accaaaggtt tcttggtgat gattttcagg ccgcttccgg
     1141 atccctcttt ggtcgagtga tccctaaaga agtttttaac gatccgatcc tccaaagaat
     1201 ggaaggatac cgtaacgatt ctaccacctt gacagagaat ttgcaaggat ttcgccaatc
     1261 cgttctccag ctcttctaac tccctgttga cgtggattct gaaggcttga aaagttctcg
     1321 tcgcgaaatg ttttctcatc ttctgacgaa cttttacagg aatgccggcc cgtaccgctt
     1381 ctacaagctc gagggtcgaa aggatcggcc gcctcaagac gatctgtttg gcgatactcc
     1441 tagaaaacgg ctcttccccg taggcgcgca ggatcttttc cagttcggct tccttcagcc
     1501 cattgaccgc atcctccgcc ttccttggac tttggaggtc catacgcata tccaagggcc
     1561 cctctttgtc gaaactgaac cctctctgct ccgcgtcgag ttgataggaa gaaaccccaa
     1621 gatcgaagag cacaccgtgc gcagcccgga ccttcaaacc ccgtaagatg agatccacat
     1681 tcgagaaagg accgtgaaag agctcaaagg acccctcgaa gggtttcagc cgtttttcgg
     1741 cataacggag gatctccaag tctttgtcga tcccgatcac cttgccgccg tgaccggcga
     1801 tgcgtttgag gatttctagc gtgtgccccc cctcgccgaa ggtgcagtcc acgtaaatgc
     1861 cggaggggtc ggtgatcagc ccttcaacca cttcctgaac catgaccggg atatgaaatt
     1921 ccggagggct tccttcaacg ggatggccgt cgactacatc ggatcctacg cgccctgtct
     1981 cgtccctcat ctcggaaggc ctatgccatt tagattgagg agggtcaaag cttcgggaga
     2041 gaaagtgtcg atcatgatcc cgccgaccgg atgagtcaat atctcctgaa gcaccaggtc
     2101 cagctgagct ctgatgagtg acaggggaga gaatccgacg ataaagccat cgttggccaa
     2161 tatgtcgaac gcctgaaggg tgtcgtagcg gacggtcttc acgtccatga accaatacca
     2221 caacgccacg ttgccgaaat agccgctgtt cagccacaac acccaatcct ggaagtgttc
     2281 gcgcagtcct ctgtctgggt tggtttctgt gaggagcaaa aatctcgtcc gtacacccaa
     2341 tccgtcacgc aaggcattga aacggatgag taaactcgtc agggcgtccg cgcgaaagtc
     2401 gtccatcgtg taaggccgcg cgtaagagtg gttcttcgcg atctcgaatt ctttcatcgc
     2461 atacggattg tacccgtata cggggctcgg atacccgaag ttctcgaagg cgaggatcaa
     2521 agggttgtac cggattaaaa tctctttcaa gagc
//
LOCUS       DAOPXN010000255         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_43042_length_2537_cov_6.159952, whole genome shotgun sequence.
ACCESSION   DAOPXN010000255 DAOPXN010000000
VERSION     DAOPXN010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43042_length_2537_cov_6.159952"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            543..1886
                     /locus_tag="PKM49_09885"
     CDS             543..1886
                     /locus_tag="PKM49_09885"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HNY83144.1"
                     /translation="MKRFVIVCLALSLVTGGFCNTITQFSSQVLTEVFANTKADIALG
                     TPITVGETVYLPLFKAETGFYAGAAGENSAAASGSGGAIKLMPFAIAMLSPTSVTVYP
                     ISNEKTFFEQLLEGLPELIPFIKELLFAFLKPTLPEAPGQLEIESESETPLPITSTVK
                     TESATYLELYTRLLTFQNEFLETAQDAYSYFLIGDPAIKGEYAENLHQAERAMMALEN
                     LILRNETLKTAYAIELLNQLKIGLFSVRNALEALFVNFEKNGEVSTADLLPLLKQLEG
                     LNRSFQQITQGIEEQVLGAPAFLESVARTISDTGLPPLITYHFAALNSDVFQFVEQVY
                     RLCLLPDAFAVSVEGLLEEKRDIEAHTAQMKTSFEHAQSEEIGDVPGLFDPMTTLFQT
                     ISELVERGFAIAQTAIAKEAPADGDILAFKTLVDIYTRLIDTIKSQLKEELVVPR"
     gene            complement(1888..2313)
                     /gene="dut"
                     /locus_tag="PKM49_09890"
     CDS             complement(1888..2313)
                     /gene="dut"
                     /locus_tag="PKM49_09890"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNY83145.1"
                     /translation="MNRTRFFAIEEALKPYKKHPEDAGWDLRAAASAEVEPWKTVAID
                     TGVSVMIPVGHVGICKARSGFGLKGLNVTAGVIDAGYRGRIHVVVQNLSDESIVIEKH
                     ERFAQILILPCLLSADFEIGEAPKDTERSASGFGSTGVK"
BASE COUNT          609 a          657 c          661 g          610 t
ORIGIN      
        1 ctaggctaga ggcaggccga ctggaagccg gccccgcgag ttatcttcga tcttttctct
       61 gtgctctctg tgccctctgt gcgaaccctg ttttttcttc gaacgaacga aaaccgggga
      121 atcgcgccat cgcgaaaaga aggggcgctt acgccgcgat gactacacgg gctaaaaatc
      181 gagcggtcga ttctggcctt gtttcttggc tcttcgacac attacttgaa gcccattgat
      241 catccaagaa atacaaagta acataagagg aataaaaaga gtccgcgaga tggccgatta
      301 tgcgattaga gcggattagc gaaacggtct aagggtctac agaattatta cgatagaaga
      361 tgttaattaa gtacgtcttg taaattgtcg aagagcctgt ttcttggtta cacctcataa
      421 cttgacatac acgatcgagc gggatgtcac ccccgcgact atggtataat ccataaggtc
      481 gtaagactgt ctaacccggt gttagggccg tatagggcca cgggtaacga ggggggatca
      541 ggatgaaaag gttcgtgatc gtgtgtttgg cgcttagtct tgtaacgggc ggcttttgca
      601 atacgataac gcagttttcg agtcaggtgt taacggaggt ttttgcgaat acgaaggcgg
      661 acatcgcctt gggaacgccg attacggtcg gtgagacggt ctatctgccc ctcttcaaag
      721 ccgaaacggg gttttacgca ggggcggcag gggagaattc tgccgcggcg tccgggtcgg
      781 gcggggccat aaaattgatg cccttcgcta ttgcgatgct ctctccgact tccgtgacgg
      841 tctatcccat ctccaatgaa aaaaccttct tcgagcagct cttagaaggg ttacccgagc
      901 tgatcccatt tatcaaagag ctgttgttcg cttttctaaa gccgacgcta cccgaagcgc
      961 ctggtcaatt ggagatagaa tcggagtcgg aaactcctct gcctatcact tctacagtta
     1021 aaacggaatc ggccacctat ctggaactgt acacgcggct attaaccttc caaaacgagt
     1081 tcctcgagac cgctcaggac gcctacagct attttttgat cggggacccg gccatcaaag
     1141 gagaatacgc cgaaaacctt catcaagccg agagggcgat gatggcgctg gaaaatctca
     1201 tcttaaggaa cgaaacgctg aaaaccgcgt acgcgatcga actgctcaat cagttgaaga
     1261 tcggcctctt ttccgtgagg aacgcgctag aggccttgtt cgtgaatttc gagaaaaacg
     1321 gagaggtttc aactgccgac ttgctcccgt tgctgaaaca attagagggc ttgaaccgga
     1381 gttttcaaca gatcacacaa gggatcgagg agcaggtctt aggggccccg gctttcttgg
     1441 agtccgtcgc ccgaacgatt tccgatactg gattacctcc tttaatcacc taccatttcg
     1501 cagcgctcaa ttccgacgtc ttccagttcg tcgagcaagt ctatcgtctg tgcctattgc
     1561 cagatgcgtt tgccgtttcc gtcgaagggt tgctcgaaga gaaacgggat atcgaagccc
     1621 ataccgctca gatgaaaacc tcctttgagc acgcgcaaag tgaggaaata ggagacgtgc
     1681 caggcttatt cgatccgatg actacgctgt tccagacgat cagcgagctc gtcgaacggg
     1741 gcttcgcgat cgcacaaacg gccatcgcaa aggaagcgcc cgcggatgga gacatcttgg
     1801 cttttaaaac gctggtcgat atctatacac gcttgattga cacgatcaag tctcaattga
     1861 aagaggaact cgtcgtcccg cgctgattca tttcacgccg gtgcttccaa acccggaagc
     1921 ggaccgctcg gtgtcttttg gcgcctcacc gatctcaaaa tctgcggaca gcaaacacgg
     1981 aaggatcaga atttgcgcga accgctcgtg tttttcgatg actatcgatt cgtcactcag
     2041 gttttggacg accacgtgga tccggccgcg ataaccggcg tcaatcacgc cggcggtaac
     2101 gttgagtcct ttaaggccga atcccgaacg ggctttgcag ataccgacgt gcccgacggg
     2161 tatcataacg gacaccccgg tatcgatcgc caccgtcttc caaggctcca cttcggcgct
     2221 cgcggccgct cgtaaatccc acccggcatc ttccgggtgc ttcttgtaag gtttcagcgc
     2281 ttcctctatg gcgaaaaacc gtgttctatt cataacgacg ctccttatgc ttcgggaaat
     2341 ccttttcgaa taggtattat acacgtcggc ttcaacctag cggcgttttg gtgtgggtaa
     2401 tatatcgagg tacaccaaaa tgggaacgcc taacttcggc tccgcctgtt ttggcttttc
     2461 tgtgtgctct cgagagccct taaacgcaat aaagccaaaa caggcggagc cgaagttagg
     2521 cgttcccatt ttggtgt
//
LOCUS       DAOPXN010000256         2445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_45403_length_2445_cov_3.626360, whole genome shotgun sequence.
ACCESSION   DAOPXN010000256 DAOPXN010000000
VERSION     DAOPXN010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45403_length_2445_cov_3.626360"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PKM49_09895"
     CDS             complement(<1..666)
                     /locus_tag="PKM49_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83146.1"
                     /translation="MGHTKTLFEETMNRIRDTGTIALLSNDSSQITPSSSAHRILTAL
                     YPAFQRVFERCGVHILKKATFQDTLQESVWSCRTFNLPFYLLLLENSESFDQVYEWLA
                     DEHSRRVFDWFVQVHMSGLFLGRFTPKIFPFVERGEAPTHPPIAVKRRGFFRYRYFVD
                     KYQFHCSEPVIEPVFLRRDYAYRDIVLPSSGESIVEGGGYYGETALWFSDAVGPAGHV
                     WSFE"
     gene            complement(1084..>2445)
                     /locus_tag="PKM49_09900"
     CDS             complement(1084..>2445)
                     /locus_tag="PKM49_09900"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNY83147.1"
                     /translation="PVVHISMTEVDTSSPDRLEESLYTLLKEIYASFDLVLDVRLVKD
                     AFRRLLSSVYQKTGQHIVLLIDEYDKPILDHLHEPAQADAIRDVLRNFYSTIKDADPF
                     LRFVFITGITKFTKAGVFSTLNNLADLTFRKAYATMLGYTQQELEDTFREYIHSSCEA
                     LDMAPSDLIEKIRRYYNGFSFDGQRFVYNPFSILSFFSESEFKNYWFESGSPYFLGQY
                     IRAHQIEFDKLTDHSVTESIFSGYEIETSPPESFLVQSGYLTFKGFHEKKGYSLDFPN
                     QEVKDAFSQLILLHQYKIDPKHNDQIRDRIVDGFEARDFSLVFAQMQQVFSGIPYPLY
                     LNRVEKKDLTGGIERLERYYHVVLLTLLWGCGIDAVAEEPSNLGRSDLAIRYGQDVWI
                     LELKKAPNPQAAKGSAQQGLEQIKEKGYANRYKGRSLFLVGIGIDEEKRNLGCYTMER
                     VSS"
BASE COUNT          602 a          672 c          564 g          607 t
ORIGIN      
        1 ttcaaacgac caaacgtggc cggcaggtcc cacggcatcc gaaaaccaaa gcgcggtttc
       61 accatagtaa cccccgccct ccacgatact ctcacccgaa gatggaagta cgatgtcccg
      121 atatgcgtaa tcccggcgta gaaagacggg ctcgataacc ggctcagaac aatgaaactg
      181 atatttgtcc acgaaatagc ggtagcgaaa gaacccgcgt ctttttaccg cgattggcgg
      241 atgagtaggc gcctcacccc tttccacgaa gggaaaaatc tttggggtaa agcggcccaa
      301 gaacaagccg ctcatatgga cttggacgaa ccaatcgaac acacggcgcg aatgctcatc
      361 ggcgagccat tcgtacactt gatcgaagga ttcgctattc tcaagaagca ggagatagaa
      421 agggagattg aaagtgcgac aggaccatac cgattcttgg agggtgtctt ggaaggtggc
      481 ttttttaaga atatgaacgc cacacctttc aaaaaccctt tgaaaagcgg gatatagggc
      541 tgtaaggatt ctgtgggcgc ttgaactcgg ggtaatttga gacgagtcat tagagagcaa
      601 cgcgatcgtc ccggtgtctc gaatgcggtt catcgtctct tcgaacaaag ttttcgtgtg
      661 ccccatactc ctatccacct cccgtgcggc aagtgcttcc tggaaacgcc ctgggaacgc
      721 ctcttgggaa cgcaggactt cagtccggca tacctgggtt ttgatttttc agctttacgc
      781 ttcttgggag cgccgcactt tagtgcggta tgtcttcttt ttcatatttg tttttttgct
      841 tctctgagtt cgcttcttgg gaactcctag gaactccgca cttcagtccg ccatatcttc
      901 tacgttttca tctttctccg tgccctccgt gttctctgtg cgaacccttc ttttttcttt
      961 ctccgtgccc tccgtgttct ctgtgcgaac ccttcttttt ctttctccgt accctctgtg
     1021 ccctccgtgc gaaccccctc ttttttcttt cgcgccctcc gtgcgaaacc attttcccgc
     1081 gttttaagaa gaaaccctct ccatcgtata acagccgaga tttcgtttct cctcatcgat
     1141 tccaatcccc acgaggaaca gggaacggcc tttatacctg ttcgcatacc ccttctcttt
     1201 gatctgctcc aggccttgtt gcgcggagcc cttcgccgcc tgaggattcg gcgccttctt
     1261 cagctccaga atccatacgt cctgcccgta tcgaatcgcc agatcgcttc ttcccaggtt
     1321 ggaaggctct tcagccaccg catcgatccc acacccccac agaagtgtca gcaacaccac
     1381 gtgataatac cgttccaatc gctcaatccc gcccgtcagg tctttcttct ctactcggtt
     1441 caggtacaac ggatacggaa tgccggaaaa aacctgttgc atctgcgcga agaccaatga
     1501 aaaatccctt gcttcaaaac catcgacgat ccgatcgcga atctgatcat tatgcttggg
     1561 atcgatctta tactgatgaa gcaagatcaa ctgactgaag gcatccttca cctcctggtt
     1621 gggaaagtcc aaagagtacc ccttcttctc gtgaaacccc ttaaaggtca gatatcccga
     1681 ctggactaaa aaactctcgg ggggagacgt ctcgatctca tagccgctga agatactttc
     1741 ggtcacggaa tgatcggtca gtttgtcaaa ctcgatctga tgggcgcgga tgtactggcc
     1801 cagaaagtaa ggggaacccg attcaaacca atagtttttg aattcgcttt cgctgaaaaa
     1861 gctcaatatc gaaaagggat tgtacacaaa ccgctgccca tcgaaagaaa agccattgta
     1921 gtaccggcga atcttctcga tcagatccga tggcgccata tccagcgcct cacaagaaga
     1981 atggatatat tcccggaagg tatcctccag ttcctgctgg gtgtacccca acatcgtggc
     2041 ataagccttg cgaaaggtca gatcggccaa attgttcaaa gtggaaaaga ctcctgcctt
     2101 ggtaaacttt gtgatccccg tgataaagac aaagcgcaaa aaaggatcgg cgtctttgat
     2161 ggtcgaataa aagtttcgaa gcacatcacg gatcgcgtcg gcctgagccg gctcatgcag
     2221 atgatccaga ataggcttgt catactcgtc gatcagcaag acgatgtgct gaccggtttt
     2281 ttggtagaca gaagaaagca aacggcgaaa ggcatccttc accagccgaa catctagaac
     2341 cagatcgaag gatgcgtaaa tttccttcag cagggtatac aagctctcct caagccgatc
     2401 aggagaagag gtatccacct cggtcatgct gatatgcacg acggg
//
LOCUS       DAOPXN010000257         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_45753_length_2431_cov_4.715067, whole genome shotgun sequence.
ACCESSION   DAOPXN010000257 DAOPXN010000000
VERSION     DAOPXN010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45753_length_2431_cov_4.715067"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKM49_09905"
     CDS             <1..221
                     /locus_tag="PKM49_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNY83148.1"
                     /translation="KTDIIEIKRYDADQYEKSQGQLKAYLERSGLSEGHLVMFSEYHE
                     AESYEKVETEGKTLHLWVIPVKRPVPSA"
     gene            323..>2431
                     /gene="pulA"
                     /locus_tag="PKM49_09910"
     CDS             323..>2431
                     /gene="pulA"
                     /locus_tag="PKM49_09910"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HNY83149.1"
                     /translation="MKKLAVVFLLLLWVLTLFAGTELIIHYHRYDGKYDGWNLWIWGE
                     GVNGQAYDFTETDAFGKTARVTLNQCFDRTGIIVRLREWMDKDVAEDRFIDTRSGHAE
                     IYILQGVKELFTSEPDISPRIFFTGAVSDRQIRAFMTNEFDTKQWEGKVSVTVNGEPR
                     AVSDVEKVIPTDISKTNFIKITLAESLSVDDLARDVRLSVEGFSPERIVMMGVLDALV
                     DEGEMGALYSKGQTTFRVWSPVSQAVSVRLYSKWDSATPDRVVAMEKNSVGTWSASIS
                     GDLNGWFYLYEYDNYGETRLGLDPYSKAASINGRKSAVVDLELTEPEGWGADGTVPFG
                     AQEDAIIYEVHIADVTGLPNSGVAKKATYLGMVETGTRGPDGVRTGIDHMVELGVTHV
                     HILPFFDFYTGEESNRDFEAMYNWGYDPYLFMCPEGRYASDPFTPETRVREVKEMVKG
                     FHNNGLNVIMDVVFPHTYGVGELSPFDQAVPYYYYKISKSGAHINESGCGNTIASQRP
                     MMRKFILDAVEYWVGAYHIDGFRFDQMGFMDIPTMMLVEERLRALNPYVLLYGEGWGD
                     ALAIDYWGWPEDDRKEGNSVTRLVKTAVAATPFAAFNDDIRDAIRGSVFEPATRGFVL
                     NALAKVKKIKSGAVGSIDFSKTEKGFADDPQQTINYAACHDNHTLWDKNTLAAKADSS
                     YAWTEADLVRAQKLAGAVLMT"
BASE COUNT          539 a          612 c          755 g          525 t
ORIGIN      
        1 ggaagacgga catcatcgag atcaagcggt atgacgcgga ccagtacgaa aaaagtcaag
       61 ggcagttgaa agcgtatctg gagcggagcg gattaagcga agggcatctg gtgatgttct
      121 ccgagtacca cgaagcggaa agctacgaga aagtggagac ggagggcaag acccttcacc
      181 tctgggtcat cccggtcaag cggcccgtgc cgtctgcgta aggattatca cgggttccca
      241 ggccgttccc agggcgttcc gacggtggtc cacatagacg ctccgcttta cagtggggag
      301 cattttatag gaggggattt gcgtgaaaaa actggcggtc gtttttttat tgcttctctg
      361 ggttctaact ctttttgcgg gtacggaatt gattatccat tatcatcggt acgacggcaa
      421 atacgacggg tggaatcttt ggatctgggg ggagggcgtc aacgggcaag catacgattt
      481 cacagaaact gacgcctttg ggaagacagc ccgggtaaca ctcaatcagt gctttgaccg
      541 aactggcatc atcgtccgtt tacgcgaatg gatggacaag gacgtcgcgg aggatcgctt
      601 catcgatacc cgttcgggtc acgccgaaat ctatatcctc caaggcgtga aggagttgtt
      661 tacctctgaa ccggatatct ctccacgtat tttctttacc ggcgcggtga gtgaccggca
      721 gatacgcgct tttatgacca acgagttcga tacgaaacag tgggagggga aggtgtccgt
      781 taccgtcaac ggggagcccc gagcggtgtc cgatgtagaa aaggtgattc cgaccgacat
      841 ttctaaaacg aattttatca agatcacatt agccgaatct ctttcggttg atgatttggc
      901 ccgggacgtc cggttgtccg tcgaggggtt ctctcccgag cgcatcgtga tgatgggcgt
      961 tttggatgcc ctcgtcgacg agggggagat gggggcgctg tactccaagg gtcagaccac
     1021 tttccgggtg tggtctccgg tctctcaggc cgtgtcggtg cgcctgtact ccaagtggga
     1081 ctccgcgact cccgatcggg tcgtggcgat ggagaaaaac tccgtcggta cgtggagcgc
     1141 atccatctct ggcgacttga acgggtggtt ctatctgtat gagtacgaca actacggaga
     1201 gacacgcctc gggctggacc cctattcgaa agccgcgtcg ataaacggga gaaaatccgc
     1261 ggtggtggac ctggagctta ccgaaccgga agggtggggg gcggacggga cggttccttt
     1321 cggcgcccag gaggacgcga tcatctacga ggtccatatc gcggacgtga cgggcttgcc
     1381 gaacagcggt gtcgccaaaa aggccactta tctgggaatg gtggaaaccg gcacgagggg
     1441 cccggatggc gtgcgcactg gtatcgatca tatggtcgag ttgggcgtca cccacgtaca
     1501 catcctgccc ttcttcgact tctacaccgg agaagaatcg aaccgtgact tcgaggcgat
     1561 gtataactgg ggttacgatc cttacctctt tatgtgtccg gaggggcggt acgcgagcga
     1621 tccctttacc cccgagactc gggtgaggga ggtcaaggag atggtcaagg gattccataa
     1681 taacggttta aacgtgatca tggacgttgt cttcccgcat acctacggcg tgggcgagct
     1741 gtcccccttc gatcaggctg ttccgtatta ctattacaaa atctctaaga gcggcgcgca
     1801 tatcaatgag agcggatgcg gtaacacgat tgcttctcag aggcctatga tgcgcaagtt
     1861 catcctcgac gccgtcgaat attgggttgg ggcctaccac atcgatggat ttcggttcga
     1921 tcagatgggg tttatggata tccccacgat gatgttggtg gaagagcggt tgcgcgcgct
     1981 gaacccgtac gtgcttttgt atggcgaagg ctggggagac gccttggcga tagactactg
     2041 gggctggccc gaagacgatc ggaaagaggg gaacagcgtt acccgactcg tgaaaacggc
     2101 cgtggcagcg acgcccttcg ccgctttcaa cgacgatatt cgtgacgcga ttcggggctc
     2161 cgtctttgaa cccgccacac gggggttcgt gctgaacgcc ctcgcgaagg tgaagaagat
     2221 caagtcgggc gcggtggggt cgatcgattt cagtaaaacc gaaaaagggt ttgccgacga
     2281 tccgcagcag accatcaact acgcggcttg ccacgacaac cacaccctct gggacaagaa
     2341 taccttagcc gccaaagccg actcgagcta tgcctggacg gaagcggatc tggtccgcgc
     2401 gcagaaactg gcaggcgcgg tcttgatgac g
//
LOCUS       DAOPXN010000258         2430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_45777_length_2430_cov_4.797474, whole genome shotgun sequence.
ACCESSION   DAOPXN010000258 DAOPXN010000000
VERSION     DAOPXN010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45777_length_2430_cov_4.797474"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PKM49_09915"
     CDS             <1..585
                     /locus_tag="PKM49_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83150.1"
                     /translation="AKRFSPFWEKEVVKVNRIPRDRKETSRDTSDVFLLPAIGGFFLA
                     FFGLLGLTLFSGCYWLKDAQVFQALGITLRAPLSMLAFFGFSFFAGVFLFIGSCRPGR
                     PILAKTLLWLSFLFSLFSFAIQFYYLFSWGFFWFGKAFFGNQPFANSKWFEYLGLLIF
                     TITLTGALVTGSSVFQRPSRTRRRTPEETAFSED"
     gene            592..2427
                     /locus_tag="PKM49_09920"
     CDS             592..2427
                     /locus_tag="PKM49_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83151.1"
                     /translation="MPLAPVRNKALLIFWILAVSLAAALSGFDQAVHTIEEYLETAPI
                     EEYARFFFSKSIEDWGEGDYGAARQYIDLAFEKPMYPMDIPKLWYFTAKMDIETGNVG
                     DAIQNLEKVIFLEPDRTEILILLKSLRSLREMASLKNPILGLDYFSETLGFVHYVEFF
                     YNPVASDRYLDELYILDRANQFVFVEGKNRSYVIDLPCEAPSGLVIDSVAGKMVLSDL
                     AEGKLHRLDLRTGIWDVLYDGFSKPFVKAVDRIGNLYVLDPPKNVVTVLSSSGKVVRR
                     IFLSDGHTPNLVTDADVDYDLLVLQDLTLKSYRILQIPTYEEMYLIPFIPGQIPVCSC
                     CDGLGNIITLWNTGELTYRDAKDSGEGAYLPIDLTNIELEGISDMDYRPPLLTFADFD
                     DHLVKSYVLARSQSEAINFIDGMYIEGDEMHIFFRNRHISGNDFGLNGPLITVSDSGG
                     LVPFNFEYIWKPVTLHRIENGARFFNHDLQNLSKNSMNILFWRYDGQEIDFDNAAPAL
                     LAKNAKLYVLSNLTVTSDLNKLARLTGGMVLSPDWLPYLEEYYHQIPEMPFSFITYRM
                     TIPFEGVKIVTVSSRIAGMDYSDSVYYTAYMVPEVKESLINSLAQ"
BASE COUNT          569 a          602 c          612 g          647 t
ORIGIN      
        1 gcgaaaagat tctcgccctt ttgggagaag gaagtcgtga aggttaaccg gataccacga
       61 gacagaaaag aaacgagtcg agatacgtcc gacgttttcc tgttaccggc catcggcggg
      121 ttctttttgg ctttttttgg gttattaggg ttgactttgt tttccggatg ttactggctc
      181 aaagacgccc aagtcttcca agcgctcggc atcacgttac gagcgcccct atcgatgctc
      241 gcgtttttcg gattctcttt ctttgccggg gtgtttctgt ttatcggttc ttgtaggcct
      301 ggccgtccta tactggccaa aactttatta tggctttctt ttctcttttc tttgttctcc
      361 ttcgccatcc aattctacta cctcttctcc tggggttttt tttggttcgg gaaggcgttt
      421 ttcggtaacc aacctttcgc gaattccaaa tggttcgaat acctcgggct cttgatattt
      481 accatcaccc tgactggcgc cctggtgacc ggcagctcgg tttttcaaag accaagccga
      541 acccgtcgga ggacgcctga agaaacggcc ttttcggagg attgaccgcc gatgcccctc
      601 gcccccgtca gaaacaaggc gctactgatc ttttggatcc tcgctgtctc cttggccgca
      661 gcgctctcgg gattcgatca agcggtccat acgatagaag agtatctgga gaccgcgccg
      721 atagaagaat atgcgcggtt cttcttttcg aagtcgattg aagattgggg agaaggcgat
      781 tatggcgccg caagacagta catcgacctc gcgttcgaga agccgatgta cccgatggac
      841 attccgaaac tgtggtattt cacagccaag atggatattg agacaggaaa tgtgggcgat
      901 gcaatacaaa atttagagaa agtgattttt ttagaacccg atcgcacgga aattcttatt
      961 ttattaaaaa gcttgaggtc cttgagagaa atggcctctt taaaaaaccc gattctcgga
     1021 ttggattatt tttctgaaac tttgggcttt gttcactacg ttgaattttt ctacaatcca
     1081 gtagcttcag atcgatatct ggatgaactc tatatcctgg accgggcaaa ccagtttgtc
     1141 ttcgtcgagg ggaagaaccg cagctacgtg atcgatcttc cttgtgaagc ccctagcggt
     1201 ctcgtgatcg attcggtagc cgggaagatg gtgctgtcgg acctagccga aggcaaactc
     1261 catcgccttg acttgagaac gggcatatgg gatgtgttat acgatgggtt ctcgaaacct
     1321 ttcgtgaaag cggtcgatcg tattgggaac ttatatgttc tagatcctcc gaaaaacgtg
     1381 gtaaccgtcc tatcttcatc tggaaaagtc gtaaggcgta ttttcttgag cgatgggcac
     1441 acgccgaact tggtcaccga tgcggacgtg gattatgacc ttttggtgct tcaggacctc
     1501 acgttgaaaa gttaccgcat cctacagata cccacgtacg aggagatgta tttgatcccg
     1561 ttcattcccg ggcagatacc ggtctgttct tgctgcgacg ggctggggaa catcatcacg
     1621 ttgtggaaca ccggcgagct cacctaccgg gacgccaagg acagcggaga aggcgcctat
     1681 cttccgatcg atttgacgaa tatcgagctc gaagggatca gcgatatgga ctatcgccct
     1741 cctctactca ccttcgccga tttcgacgat cacctcgtga aaagctacgt actcgccaga
     1801 tcccaatctg aagcgatcaa cttcatcgac gggatgtata tcgaaggcga tgagatgcac
     1861 atctttttca ggaatcggca tatcagcgga aacgatttcg ggctgaacgg gccgctcatc
     1921 accgttagcg atagcggcgg gctggtcccg tttaacttcg agtatatatg gaagccggta
     1981 accctccata ggatagaaaa tggcgcccgg tttttcaatc acgacctaca aaacctttcg
     2041 aaaaactcca tgaatatcct cttttggagg tacgacgggc aagaaataga ctttgacaat
     2101 gcggcgcccg ccctgttggc aaagaacgcg aagctgtacg tgttaagcaa cctcaccgtg
     2161 acctccgacc tgaacaagtt ggcgcgcttg accggcggga tggtcctctc acccgattgg
     2221 ctgccctacc ttgaagagta ctatcaccaa atacccgaaa tgcctttcag ctttatcacg
     2281 tatagaatga ccattccctt tgaaggggtg aaaatcgtga ccgtctcctc ccggattgcc
     2341 gggatggact attcggattc ggtttactac accgcctata tggttcctga agtgaaggag
     2401 agcctgataa acagtttggc gcaatgaggg
//
LOCUS       DAOPXN010000259         2408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_46418_length_2408_cov_4.088823, whole genome shotgun sequence.
ACCESSION   DAOPXN010000259 DAOPXN010000000
VERSION     DAOPXN010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46418_length_2408_cov_4.088823"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="PKM49_09925"
     CDS             <1..1500
                     /locus_tag="PKM49_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNY83152.1"
                     /translation="RVSLGLSRMVKSAIIAYDTRHRSRYFALLAARTLAAQGVRVLVF
                     DEPAPTPVLSFAIRRLKTQSGLVITASHNEPAYNGIKVYDNRGVQMVPSRIETLKTHL
                     ESFGFFERVPEAYAYDFLGEEIKTEYLKEVSRENAPFDLPDGRRHLILYTPLHGTGGA
                     YIPPLLDRFGFDCRVVEKQSRADPTFSTVRYPNPEDPEAFGLALTQAKGMHPMPEMIL
                     ATDPDADRIGVYLLENDSYRPLSGNEIGLLLTDFLVRATPTHPERTEDRTRVLLKTIV
                     TSDSVKPYALKNRIEVIETLTGFKFLGDRIEAINGENKEFLAAYEESYGFLHGDHARD
                     KDALSTALLLGLLLRNLGSASRMIDRLQEIYREFGFYRDSAETQRFEGSEGARRIEEF
                     MSALRAADPHRLFSLPVEAVRDYNVEVDPLWRADVFLVRFNQGMKAIFRPSGTETKLK
                     IYLSAVGKTQEECEGRYHTLKTEITRAASGMLASLTTADPSLKDQQNRE"
     gene            1508..2053
                     /gene="hpt"
                     /locus_tag="PKM49_09930"
     CDS             1508..2053
                     /gene="hpt"
                     /locus_tag="PKM49_09930"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HNY83153.1"
                     /translation="MEIKRESIEVLYDRQTIQNRVAELGREIARDYAPVTDTLYMVCV
                     LKGSTHFFSDLLLNIPIDVHYMFIQIASYTGSSSTGQIVVKSWIEESVQGKHVIVVED
                     ILDSGKTLKYVLNYLSKYHPETLEVATLVDKVSKDHYGIKAKYVGFNAPDYFLLGYGL
                     DYNQRFRNLPFIGYVRHDVET"
     gene            2037..2321
                     /locus_tag="PKM49_09935"
     CDS             2037..2321
                     /locus_tag="PKM49_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83154.1"
                     /translation="MTWKRSGVLILLTVFVLFVFSVSFLWYLDDVQSQENLLVSMEIA
                     KWRSLFSLQLYIRVGELYRQGLLSNCDSEIAINGHTITVTPKGVLFVKGE"
BASE COUNT          587 a          618 c          658 g          545 t
ORIGIN      
        1 cgggtttctt tggggctttc tcggatggtg aaaagcgcga tcatcgccta tgacaccagg
       61 caccgttcgc gctatttcgc tttgctggcc gctaggacct tggcggcgca aggagtcaga
      121 gttctcgtgt tcgacgagcc ggcgccgacg cctgtccttt ctttcgccat cagacggttg
      181 aaaacccagt cgggcttggt catcaccgcc agccataacg aaccggcgta caacggcatc
      241 aaggtctacg ataatcgtgg ggtgcaaatg gtcccgagcc ggattgaaac cctaaaaacg
      301 catctcgagt ccttcggttt cttcgagaga gtcccggaag cctatgccta cgacttcctc
      361 ggggaggaga tcaaaaccga atacctgaaa gaggtgagcc gggaaaacgc gccgttcgac
      421 ctacccgacg ggcgaaggca tctgattttg tacacgccgt tgcacggaac gggcggcgcc
      481 tacattcctc ccctgctgga tcgcttcggt ttcgattgcc gagtggtcga gaaacagagt
      541 cgggcggatc ccaccttctc cacggtgaga tatccgaatc cggaggatcc ggaggctttc
      601 gggttggcgc ttacacaagc caaaggcatg cacccgatgc cggaaatgat cttggcgacc
      661 gatcccgatg ccgacagaat cggggtctac cttttagaga acgattccta ccgcccctta
      721 agcgggaacg agatcgggtt gctcctgacc gattttttag tgcgcgccac gcccacccac
      781 cccgaaagaa ctgaagaccg aacccgagtc ctcctcaaaa ccatcgtgac gtcggacagc
      841 gtgaaaccct acgcgctcaa gaaccgtata gaggtgatcg aaacgctgac cggtttcaag
      901 ttcctgggag atcgaataga agccataaac ggcgaaaaca aggaattttt ggccgcttac
      961 gaagagagct acggcttttt gcacggagat cacgcccggg acaaagacgc cctttccacc
     1021 gcgctcctgt taggcttgct gttgaggaac ctggggagcg cgtcgagaat gatagatcga
     1081 ctgcaggaaa tctatcgaga attcggtttc tatcgtgata gcgcggagac acagcgattc
     1141 gaggggtcgg aaggtgcccg tcggatcgaa gagtttatga gtgctctgcg agcggccgac
     1201 ccacatcgtt tgttttcatt gcccgtggaa gcggtcagag actataacgt ggaggtcgac
     1261 cccctatgga gggcggacgt gtttctcgtc cggttcaatc aggggatgaa ggcgatcttt
     1321 cgcccttccg gaaccgaaac gaagctcaag atctatttgt ccgctgttgg aaaaacccag
     1381 gaagaatgcg aagggcgcta ccacaccctt aaaaccgaga tcacccgggc cgcttccggg
     1441 atgttggcat ccctgacaac ggctgacccc tcattaaaag accagcagaa tcgggagtaa
     1501 tcggcgaatg gaaattaaaa gagaatcgat cgaagtgctc tatgaccgac agacgatcca
     1561 gaatagggtc gctgaactcg ggagggaaat cgcgcgggat tacgcgcctg tgaccgatac
     1621 tttgtatatg gtctgcgtgt tgaaagggtc gacccacttc ttttccgacc ttctgctgaa
     1681 tattcctatt gacgttcatt atatgttcat tcagatagcc agttacaccg gctcttcctc
     1741 taccggacaa atcgtggtaa aatcttggat tgaagagtcg gtgcaaggga aacacgtcat
     1801 cgtggtggaa gacatattgg actcgggaaa gacgctgaag tacgtcctga attacttgtc
     1861 caagtatcac ccagaaactt tggaggtggc gaccctggtc gacaaagtat ccaaagacca
     1921 ttacgggata aaagccaaat acgtggggtt caacgctcca gactactttt tgctggggta
     1981 cgggctcgat tacaatcaac gattccggaa ccttcccttc atcggatacg ttaggcatga
     2041 cgtggaaacg tagcggtgtc ctcatcctgc tgaccgtttt cgttctcttc gtcttttccg
     2101 tgtcatttct gtggtacttg gacgatgttc aaagccaaga aaacctgctc gtttcgatgg
     2161 aaatcgcaaa atggcgttcg cttttttctt tgcagttgta tataagagtg ggagagctat
     2221 atagacaagg cctgttgtca aactgcgatt ctgagatcgc gattaacgga cataccatca
     2281 cagtcactcc gaaaggcgtg ttgttcgtga aaggagaata aacggtgtcg gatgagatcg
     2341 tttcgaaaga gcctatcccg aaccgaacgg attcgatgac gcccaagagc atcgtggcga
     2401 ttttggac
//
LOCUS       DAOPXN010000260         2401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_46626_length_2401_cov_3.253623, whole genome shotgun sequence.
ACCESSION   DAOPXN010000260 DAOPXN010000000
VERSION     DAOPXN010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46626_length_2401_cov_3.253623"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PKM49_09940"
     CDS             <1..670
                     /locus_tag="PKM49_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83155.1"
                     /translation="SVVTTPTPNTFSIQWHPVFSNDVQEQYRLFDLNSPGQFSTSTAA
                     LKTVSKSVTQTTFSTTKSHIALCAVNTSAGYPNTFFSSPTFFTLPASSLPQLFFQSPT
                     YSVPVGGTITVRLLAQGLPSFQTIELLLGFTGDLHLSEVTAGTVFNTNARLLNGQNLS
                     EFYVHATHLYGQSNALFEGALLTCTVVANTSGSLFFNDARAIDASLNVSTLQTGSPVT
                     IQVY"
     gene            685..>2401
                     /locus_tag="PKM49_09945"
     CDS             685..>2401
                     /locus_tag="PKM49_09945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HNY83156.1"
                     /translation="MKRTFLWVLLIGVPILSLFAVPAFPEPFEVVQPDGYRFLVQIIG
                     DEFFHYIIETESGCVIRLDEESNYWVHAPLPGVEDDYPFLPVQGGSAGRIAPPSKEAI
                     EAAREKALTSGNFYLEASQRKRSAPTTGTVKIPVILINFSDRSTMYTNAQFDQLIFGN
                     YPALAPNGSMKDYYNEVSYQQLTLTGSVLGWYTSAHGHDYYGQNDANGQDFDPRALVT
                     EAVLAADPFVDFSQFDNDSDGFVDMVIVVHQGRGEEESGVPTDIWSHQWSLNYPGLLV
                     DGVRVSQYTLQPEKYQSQMTAIGVIAHETGHAFGLPDLYDYSNATNGIGRWGIMSSGS
                     WNYLSQPGDCPAHFCAWSKKDLGWLTVDSVNGYSGSKTLLPSVTSKTVLKYANPSDAL
                     EYFLMENRKLAGFDRGLPGAGMLLLHVDDKVDNNDLGAWSHKRVDVEEADGLNHLDTK
                     ANSGDAGDVFPGTSNNTAFNDTSNPNAKWYNGSNSALQITQIQKTGDQISMNFTAPVS
                     TYSLTVQTAPFSSMDISIAGSPYLSPKTVTLPAGTYQVQIPLSNRDESSLVPGTDTRY
                     SLLQWE"
BASE COUNT          567 a          619 c          595 g          620 t
ORIGIN      
        1 ttcggttgtc acgaccccca cgccgaatac tttttcgatc caatggcatc cggttttttc
       61 caacgatgtc caagagcaat accgactttt tgatttgaac agcccaggcc agttttcaac
      121 gtcaacagcc gcattgaaga cggtgtctaa gtcagtcact caaaccacgt tctcgacaac
      181 gaaatcgcat atcgctctat gcgccgtcaa tacatcggca ggctacccaa acactttttt
      241 cagctctccg acgtttttta cgctccccgc gtcttctcta ccgcaactgt ttttccaatc
      301 gccgacctat tcggttcccg tgggcggtac gatcaccgtg cgtttactgg ctcaagggtt
      361 gccttctttt cagacaatcg agctgttgct gggcttcaca ggcgatttac atctatccga
      421 agtaactgca gggacggttt tcaatacgaa cgcgcggtta ttgaacggcc aaaatctttc
      481 cgaattttac gttcacgcga cgcacttgta tggacagagc aacgcgctct tcgaaggcgc
      541 gctattaact tgcacggtcg tggcgaatac ctcggggagc ctgttcttta acgatgcgcg
      601 ggcgatcgac gcctctttga atgtgtctac cctgcaaacg gggtcaccgg tcaccatcca
      661 agtctattaa gggagggatt cgagatgaaa agaacgtttc tatgggttct actgataggt
      721 gttcctatct tatccctttt cgcggttccc gcttttcccg aaccattcga agtcgttcaa
      781 cctgacggat atcgtttttt agtgcaaatc atcggagatg agtttttcca ttacatcata
      841 gaaacagaaa gcggttgcgt gatccgcttg gatgaggaat cgaactactg ggttcacgcg
      901 ccattacctg gggtcgaaga cgactacccc ttcttgccgg ttcaaggagg ttctgccggg
      961 cgtatcgcgc ccccttctaa agaggccatc gaagcagcgc gtgaaaaagc cttgacaagc
     1021 gggaattttt atctcgaagc ctcccaacgc aaacgttccg cgcctacgac cggaacggtg
     1081 aagatccctg tgattctcat caacttttcc gatcgatcaa caatgtatac caacgcgcaa
     1141 ttcgatcaat tgatatttgg taactaccct gcccttgcgc ctaatgggtc gatgaaagac
     1201 tactacaatg aagtgtctta tcagcagtta acgctaacgg gttcggtatt aggctggtat
     1261 acttcggcgc acggacatga ttattatggt cagaacgatg caaacgggca ggattttgac
     1321 cctcgcgccc tggtgaccga agccgtcctc gcggccgacc ccttcgtcga tttttctcaa
     1381 ttcgataacg actccgatgg tttcgtggat atggttatcg tggtccatca aggccgagga
     1441 gaagaagaat cgggggttcc cacggacatt tggtctcatc aatggagctt gaactatccc
     1501 ggtttattgg tggacggggt tcgcgtatcc cagtatacgc tgcaacctga gaagtaccag
     1561 tcacaaatga cggcgattgg tgtgatcgcg cacgagacgg gacatgcgtt tgggttgcct
     1621 gatctttacg attattccaa tgcgacgaac gggatcggac gatgggggat tatgagttcc
     1681 ggaagctgga attatctaag ccagcccgga gattgcccgg ctcatttttg cgcttggtca
     1741 aaaaaagacc tcggttggct gaccgtcgat tccgtgaacg gatacagcgg gtctaaaacg
     1801 ctgttgcctt cagttacaag taaaacggtt ttgaaatatg cgaacccttc agatgcatta
     1861 gagtatttcc tgatggaaaa tcgtaagctc gcgggtttcg atcgaggact tcctggcgcg
     1921 ggtatgcttc tgttgcacgt cgatgacaag gtggacaata acgacttggg cgcttggtca
     1981 cataaacgcg tagatgtgga agaggcagac gggttgaatc atttggatac gaaggccaac
     2041 tccggcgacg ccggtgacgt gtttccggga acttccaaca acacagcttt caacgatacg
     2101 agcaatccga acgctaaatg gtacaacggt tctaacagcg cgctgcagat tactcagatt
     2161 caaaaaacgg gagatcagat cagtatgaac ttcacggcgc ctgtttcgac ctattcgctt
     2221 accgttcaaa cggctccttt ctcttcgatg gatatctcta tcgccgggag cccttacctt
     2281 tccccaaaaa ccgtgacgtt gccggccggc acctatcaag tgcagattcc tctgtctaac
     2341 agagacgagt cttctcttgt tccgggaact gacacgcgtt attctctcct gcaatgggaa
     2401 g
//
LOCUS       DAOPXN010000261         2372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_47432_length_2372_cov_4.705222, whole genome shotgun sequence.
ACCESSION   DAOPXN010000261 DAOPXN010000000
VERSION     DAOPXN010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47432_length_2372_cov_4.705222"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..690)
                     /locus_tag="PKM49_09950"
     CDS             complement(181..690)
                     /locus_tag="PKM49_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNY83157.1"
                     /translation="MKEMTKRFLSDAFAGESQAHMKYLIFAEEAAKKGLPRLANLFRA
                     IAKAEYVHAKNHFVALGHLGSMEQNLQAGIDGESFEIAEMYPVYLEAAKLQGEKEAVR
                     SNHFALEAEKIHEKMYEKAKALVREGKDYTAAKVYICPVCGHTTEDGVPDKCPVCGVP
                     GSKFIAFEA"
     gene            1347..1811
                     /locus_tag="PKM49_09955"
     CDS             1347..1811
                     /locus_tag="PKM49_09955"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNY83158.1"
                     /translation="MTNPYEGNDVLATPVVFESLFEIVSYFADYFKPAVQMRNLKTSE
                     LFILLFLYLKGPKSMNQLSRFLNTTKSNMTALIDQMERRDLLKREHSKVDRRVIDVSL
                     KAKGRQICEEASDGFNVLVQAFFKKVNKEDLPIISQGFMCLHRLATESVTEG"
     gene            1812..>2372
                     /locus_tag="PKM49_09960"
     CDS             1812..>2372
                     /locus_tag="PKM49_09960"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNY83159.1"
                     /translation="MKELVYIDCGTSQLRVFSSLRGIVWDLPSYLAIENDRIFAYGEE
                     AKALAGKTGNAFGLTPRSFIAQPVQKGSLFYDGLLASWLEILLQKERMLGLFKKPTVV
                     FSVSPFINTVEKRAFMNIAHSIRIPHAFLLPHAYAIYGGYQIDFRENKARLVIDIGAG
                     KVDIAVLSSGRVIAGKTKKGLLEEKQS"
BASE COUNT          541 a          605 c          609 g          617 t
ORIGIN      
        1 gactcccttc cgcgaccatt ccagaagttc gggcgggatc gcgccctttt ctccattcgt
       61 tccggctcca aagacaaaaa aacgtcccgc tatttcgcgt attccttttc tcacatttct
      121 ccctcccaaa gaaagagacg ccgcaacgcg cgtctcctat tgatcgatta catcgctcgt
      181 tcaggcttcg aaagcgatga atttggatcc gggcaccccg cagaccgggc acttgtccgg
      241 gactccgtct tccgtggtgt gaccgcaaac ggggcaaatg tacactttcg cggccgtgta
      301 atcttttcct tcccgaacca gggctttcgc tttttcgtac atcttctcgt ggatcttttc
      361 ggcttccaag gcaaaatggt tgctgcggac ggcttccttt tctccctgca gcttagcggc
      421 ttccaaatag acggggtaca tttcggcgat ctcgaagctc tctccgtcta tcccggcttg
      481 caagttttgc tccatcgagc ctaagtgccc caaggccacg aaatggtttt ttgcgtggac
      541 gtactcggct ttcgcgatcg ctcgaaacag gttcgccaat ctcggtaagc cttttttggc
      601 cgcttcttcc gcgaaaatga ggtatttcat atgcgcttgg gactctcccg cgaaagcatc
      661 ggatagaaaa cgtttagtca tttctttcat agtttgctcc tcctgtatca aatgagattc
      721 tcgtgagatt ctcaagtttt gtcgcgttgg ctctcgatta taacacgatt ttgaaattcg
      781 aataaaaggc gaatgtgtca atttatgggg tgtactctaa aaatgtggcc agaatcgatc
      841 gcgcgatttt tcgtttgtca tcgcggcgta agcgcttctt ctttttgtgg ggccgacttc
      901 cagtcggcct gcctctctgg gaactccaac ttataagtta gcatgtcttg agaatagaac
      961 agagttctcg acggagaaca cagagaacgc gaaggaaaca taggacacat gccgaactaa
     1021 agttcggcgt tcccaggggc gctcggggaa aaaacttgcc gaactgaagt tcgcgttccc
     1081 aggggcgttc ggggaaaaaa cttgccgaac tgaagttcgc gttcccaggg gcgttcgggg
     1141 aaaaaacttg ccgaactgaa gttcgcgttc ccagcggcgt tcccgggagc tcggcgctcc
     1201 agaagcgagg cccctggccg ttcggttttt tctgtttctg tgctataatg ttctatacgc
     1261 tgctctgatc aagcccagaa gggttttttt cgttccgacg tcagggtggg gaaggcgcct
     1321 taacggcttt ctgaaggagg aatgggatga cgaatccgta cgagggaaac gatgtgctgg
     1381 cgactcccgt cgttttcgaa agcctcttcg aaatcgtcag ttactttgcc gattatttta
     1441 aaccggccgt tcaaatgaga aatctgaaaa cttccgaact gtttattctg cttttcctct
     1501 atctcaaggg cccgaagagc atgaatcagc tttcgagatt tctcaatacc acgaagagca
     1561 atatgaccgc tttgatcgat cagatggaac gccgggattt gctcaaacgc gagcattcca
     1621 aagtggatcg acgagtgatc gacgtctcct tgaaagcgaa aggaaggcag atctgcgaag
     1681 aagcctccga cgggttcaac gtgctggttc aagcgttctt caaaaaagtg aataaagagg
     1741 atctgcctat catatctcaa gggtttatgt gccttcatcg gttagcgaca gagtccgtca
     1801 cggagggata gatgaaagaa ctggtctata tcgattgtgg cacgtcgcaa ctacgcgtct
     1861 tttcctcctt gagagggatc gtttgggatc tcccatctta tctggcgatc gaaaacgacc
     1921 gtatcttcgc ctacggggaa gaggctaaag cgctcgccgg aaagaccgga aatgccttcg
     1981 gcctgacgcc gaggtccttc atcgcccagc cggttcagaa gggcagtctc ttttacgacg
     2041 ggttgttggc ttcttggctg gagatcctgc tccagaaaga aaggatgtta ggcctgttca
     2101 aaaagccgac cgtggtcttt tccgtctcgc cctttatcaa tactgtagaa aagcgagcct
     2161 ttatgaatat cgcccattcg atccggattc ctcacgcctt tcttttaccc catgcctacg
     2221 cgatatacgg tggatatcaa atcgatttcc gagagaacaa ggcgcgactg gtcatcgata
     2281 tcggggccgg aaaggtagac atcgccgtac tgagttccgg ccgggtcatc gcggggaaga
     2341 cgaaaaaggg gctgttggaa gagaaacagt cc
//
LOCUS       DAOPXN010000262         2346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_48208_length_2346_cov_4.984286, whole genome shotgun sequence.
ACCESSION   DAOPXN010000262 DAOPXN010000000
VERSION     DAOPXN010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48208_length_2346_cov_4.984286"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PKM49_09965"
     CDS             complement(<1..474)
                     /locus_tag="PKM49_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY83160.1"
                     /translation="MKLRQKKTMTAYLFLLIPLVFFVFVRFVPTIYAFWLSLTDWNII
                     SPKMNFVGLDNFRELFSDTVFLKALGNTFKYVLYGVPLVLALSFVISLLLNAIQKGQA
                     FYRLIYVMPYITPLVAVSWVWRWMYQRPPIGVINGLLSFVGLPTQRFLFSMDQALI"
     gene            complement(606..1847)
                     /locus_tag="PKM49_09970"
     CDS             complement(606..1847)
                     /locus_tag="PKM49_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HNY83161.1"
                     /translation="MKRSFLILLLCVALLATLSFAKTKIVYWQYFYETKIQTVDQVIK
                     EFERLNPDIEVEHVNFPYENFNQKVAASVPAGIGPDVVNLFYGWIPKYVTSGYLQPLP
                     EVEFSKEYFAENFFPFVGPGVAYEGKSYALPIAVRSLALIWNKDLFEAAGLDPETPPV
                     TLNELVAYAKKLTVYDRAGNIVQAGLTMQPSGQGHQWIREVLIRQFGGVPYSNDGKMV
                     MYDKTGAGPDVLKFYTDLIVKDNISYPGFMNDDVTAFSSQKAAMTIDGSFRIGTLKNV
                     KGLNFAFTELPSHNGVKSNFASFWANGITKNATGDRLTAAIKFIKFLSSEYVMDLWLK
                     NVGELPANPALASKYYDDPTYGPFLKGLEYAHATFFVDETAQRQVIMDAVDKVINGYS
                     PQAAWKEMAAEEQKILDKFWK"
     gene            complement(1862..>2346)
                     /locus_tag="PKM49_09975"
     CDS             complement(1862..>2346)
                     /locus_tag="PKM49_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNY83162.1"
                     /translation="VYEGAHGYAGEIGYQVTDWNREVANTGQGAFGSLENWFSGYHLE
                     KRLEKMGRKLTIEAFFEDAQTDTELAALLSEAVEHLALAISNAIVLLDPESVILTGGI
                     GFHQYDRLIRMMWPALQRTVPEEMLDQIVFRKGALGKDGVLVGAVSYVHENVIVSTTS
                     "
BASE COUNT          563 a          628 c          603 g          552 t
ORIGIN      
        1 gatcagggct tgatccatac tgaacaagaa ccgttgcgtg ggcaggccga cgaaggacaa
       61 tagtccgttg atcaccccga tcggcggcct ttggtacatc catcgccaca cccagctcac
      121 ggcgacgagc ggcgtgatat acggcatgac gtaaatcaaa cggtagaagg cttgcccttt
      181 ctgaatggcg ttcaagagca aagatataac aaacgataag gccaacacga gaggcacccc
      241 gtagagcacg tatttgaagg tattccccaa agctttcaag aaaaccgtat cggagaagag
      301 ctctcggaaa ttatctaagc cgacaaagtt catcttcggt gaaatgatgt tccaatcggt
      361 caaggagagc caaaaggcgt agatcgtcgg gacaaaccga acgaagacga agaaaacgag
      421 ggggatcaag aggaagaggt aggccgtcat tgtcttcttt tgacgcagtt tcataccatc
      481 accgccccga aggataaaaa aaactaacat ataagcgatg ggccgccttt aaaatagcgg
      541 gccgcttaga aaggcgggcc ttcgccggga tgtcgcggtc tagacccgcc tcgttcctta
      601 agacgctact tccagaattt atccagtatt ttttgctctt ccgcggccat ctctttccag
      661 gcggcttgcg gggaataccc gttgatgact ttgtcgacgg cgtccatgat cacctgtcgt
      721 tgcgccgttt cgtctacgaa gaaggtggcg tgcgcgtact ccagaccctt gaggaacgga
      781 ccgtaggtgg gatcgtcata gtatttggac gctaacgccg ggtttgcggg cagctccccg
      841 acgtttttaa gccacaggtc catcacgtac tccgaagaaa ggaatttgat gaacttgatc
      901 gccgcggtca aacggtcgcc ggtcgcgttt ttcgtgatgc cgtttgccca aaaggaagcg
      961 aagtttgact tcacgccgtt gtgagatggt aactcggtga aggcaaaatt gaggcctttc
     1021 acattcttta aggtgccgat gcggaacgat ccgtcgatcg tcatcgcggc tttttgagaa
     1081 gaaaaagcgg tcacgtcgtc gttcataaag ccaggatagc tgatgttgtc tttgacgatc
     1141 agatcggtat agaacttcaa aacatccggt ccagcgccgg tcttgtcgta catcaccatc
     1201 ttcccgtcgt tcgagtaggg cacgcccccg aactgtcgga tgagtacttc ccggatccac
     1261 tgatgtccct gaccggaagg ctgcattgtc aatccggcct gtacgatatt cccggctcga
     1321 tcatagactg tcagtttttt cgcgtaagcg actaattcgt tgagcgtgac gggcggagtc
     1381 tcggggtcca atcccgcggc ctcgaacagg tctttgttcc atatcaacgc caggctgcgg
     1441 accgcgatag gcaaagcgta gcttttccct tcgtaagcga cgccgggccc cacgaatgga
     1501 aagaagtttt cggcgaagta ctctttggaa aactccacct ccggaagcgg ttgcagatag
     1561 cctgaggtca cgtacttagg tatccatccg tagaacaggt tcacgacatc cgggccgata
     1621 ccggcaggca ccgacgcggc tactttttgg ttgaagtttt cgtagggaaa gttcacgtgt
     1681 tcgacttcga tgtccggatt cagacgttcg aactccttga tcacttggtc aaccgtttga
     1741 atcttggtct cgtaaaagta ctgccagtaa actatcttcg tcttcgcaaa agaaagagta
     1801 gccaggagcg ctacacacaa caacagaatc agaaagcttc ttttcatacc gcacctccat
     1861 tttaagatgt cgtactcacg atcacgttct catgcacata agaaacggcg ccgaccaaaa
     1921 cgccgtcctt cccaagggcg cctttccgaa atacgatttg atcgagcatc tcttcgggga
     1981 ccgtccgttg gagcgccggc cacatcatcc ggatcaggcg gtcatattga tggaaaccga
     2041 ttcctccagt gagtatcacg ctttccgggt caagtaggac gattgcgttg gaaatagcta
     2101 aggccaagtg ctcgaccgct tcactcagaa gcgccgcgag ctccgtgtcc gtttgcgcgt
     2161 cctcgaagaa ggcttcgatc gttagttttc gccccatttt ctctagacgt ttttctaaat
     2221 gataccctga aaaccagttt tcgagagacc cgaaggcgcc ttgccctgtg ttggcgacct
     2281 ctcggttcca atcggtcact tgatacccta tctctcccgc gtacccgtgc gccccttcat
     2341 agaccc
//
LOCUS       DAOPXN010000263         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_49022_length_2319_cov_3.918728, whole genome shotgun sequence.
ACCESSION   DAOPXN010000263 DAOPXN010000000
VERSION     DAOPXN010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49022_length_2319_cov_3.918728"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="PKM49_09980"
     CDS             <1..1156
                     /locus_tag="PKM49_09980"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HNY83163.1"
                     /translation="NKNVPLLFEMTAKYGTPYFQNFINSDLNPGDVRSMCCRLQLDKR
                     ELRRRGGGLFGSDEFTGSIGVVTLNLPRIAYLSLNERDFFTRLGKLMDIAKRSLEIKR
                     RIVAKFNELGLYPYTKRYLNHFDNHFSTIGLVGMNEATLNAVWIGQPLYEPEARAFGL
                     KVLDFMRERLARYQEETGNLYNLEASPAESASYRLAKHDKKAYPQILSAGKETPYYTN
                     STHFPVNYTEDVFEALDQQEAFQTKYTGGTVFHAFLGERLYDWETARELVKKIATLYR
                     IPYFTLSPTYSICQNHGYLNGEVHACPKCGNPTETYSRITGYYRPIQHWNAGKQSEFA
                     DRRTYHYPEVAGSRPLGGERTDEFQRVATGQLGRLSENHQHSRLYVEVQL"
     gene            1068..1727
                     /locus_tag="PKM49_09985"
     CDS             1068..1727
                     /locus_tag="PKM49_09985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY83164.1"
                     /translation="MNFSGWQRVSLVDYPKTISTVVFTSKCNFDCAYCHNPSLKTAQP
                     ATHSEAAILEYLARRHKQIDALVISGGEPSLRAEEIIGFCHRLKTALPNLLIKVDTNG
                     SNPLFLQEGRDVFDYVAMDVKSLHYDRFSTTPFDTILQSLDLLDSYPRHEVRVTVYPP
                     YLPETDFEELGRLLKDKRVRHVTLQQYRPVDDIPPYPAETLHTFATLLQTLGLETSLR
                     T"
BASE COUNT          578 a          723 c          601 g          417 t
ORIGIN      
        1 caacaaaaac gtaccgctac tgttcgagat gacggccaaa tacggcacgc cttattttca
       61 aaacttcatc aactccgatc tcaacccggg cgacgtccgc agcatgtgct gtcgtctcca
      121 gctggataaa cgcgaactgc gaagaagggg cggcggattg tttggttccg acgaattcac
      181 cggctcgatc ggtgtggtga cgctcaacct gccacgaata gcctacctga gcctgaatga
      241 acgggatttc tttacgcgcc tcggcaagct gatggatatc gccaaacgca gcctcgagat
      301 caaacggcgc atcgtggcga agttcaacga actcgggcta tacccctaca cgaagcgata
      361 cctcaaccac ttcgacaacc acttctccac gatcgggctt gtcgggatga acgaagcgac
      421 actgaacgcc gtttggatcg gtcaaccgct gtacgaacca gaagcccggg ccttcggttt
      481 gaaagtcttg gactttatgc gggaaaggct cgcgcgatac caggaagaga cgggaaacct
      541 gtacaacctg gaagcctcgc ctgcagagtc cgcttcctac cgattggcca agcacgacaa
      601 aaaggcctac ccccaaatcc tctccgcggg aaaggaaacg ccgtactaca ccaactccac
      661 gcacttcccc gtgaactata cggaggacgt cttcgaagcc ttggaccagc aggaggcctt
      721 ccagacgaaa tacacgggcg gcacggtctt ccacgctttc ttgggagaac gcctgtacga
      781 ttgggaaacc gccagggagc tcgtgaagaa gatcgccacg ctgtaccgca tcccctactt
      841 cacgctcagt ccgacgtact cgatctgtca aaaccacggc tatctgaacg gagaggtcca
      901 cgcttgcccg aaatgcggaa acccgacgga gacctactcg cgcatcaccg gatactaccg
      961 gccgatccaa cactggaacg cgggaaaaca aagcgaattc gccgatagac gaacctacca
     1021 ctatccggaa gtggcaggct caaggccatt gggaggcgaa aggaccgatg aatttcagcg
     1081 ggtggcaacg ggtcagcttg gtcgattatc cgaaaaccat cagcacagtc gtctttacgt
     1141 cgaagtgcaa ctttgactgc gcttactgcc acaacccgag cctgaagacg gcgcaacccg
     1201 ccacacacag cgaagccgcg atcctcgaat acctcgcgcg cagacacaaa cagatcgatg
     1261 cgttagtgat atccggaggc gaaccgtctt tacgcgccga ggagatcatc gggttctgtc
     1321 atagactgaa aaccgcgctg ccaaacctac ttataaaagt cgacacgaac gggtcgaacc
     1381 cgctcttcct ccaggaaggg cgggacgtat tcgattacgt ggcgatggac gtaaaaagcc
     1441 tgcattacga caggttcagc acgacaccgt ttgacacgat cctacagagc ctggacctcc
     1501 tcgactcgta tccgcgccac gaagtccgcg tcacagtcta cccgccctac ctacccgaaa
     1561 cggacttcga agagctggga cggctgctaa aagacaaacg cgtccgacac gtgaccctcc
     1621 aacaataccg ccccgtcgac gacatccccc cctatccggc ggaaaccctc cacaccttcg
     1681 cgaccctcct acaaaccctc ggcctcgaaa cctccttaag aacctaaccc cacaagggca
     1741 tcgcggcgta agcgctcctt cttttcgcga cagcgcgata cgcttttgta tatgtcttga
     1801 aaaacagggt tcgcacagag ggcacagaga acacagagaa aagacgggag aaagctcagt
     1861 cgagccggct tccagtcggc ctgtatctct ggaaagattc cgggagcgcc tggacgttcc
     1921 aaagatcgaa ttgcagagtc tcgctggtta acccgcgata tccccataag ggcatcgcgg
     1981 cgtaagcgcc ccttcttctc gcgacggcgc gatacgcttt tgtgcacggg catcgcggcg
     2041 taagcgcccc ttcttttcgc gacggcgcga tacccggact gaatcgcggc gtaaagaata
     2101 caaaaggcgg atcgcggggt cccttcggtt accccgcgat aaccccatgg cgcgataccc
     2161 ttttgtacag ccggatcgca gagcttcgtc aggatcgcgg ggtcccgttg gttaccccgc
     2221 gatgacccaa cacgggcatc gcggcgtaag cgccccttct tttcgcgacg gcgcgatacc
     2281 cggactgaat cgcggcgtaa agaatacaaa aggcggatc
//
LOCUS       DAOPXN010000264         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_49420_length_2305_cov_5.258667, whole genome shotgun sequence.
ACCESSION   DAOPXN010000264 DAOPXN010000000
VERSION     DAOPXN010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49420_length_2305_cov_5.258667"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1495
                     /locus_tag="PKM49_09990"
     CDS             212..1495
                     /locus_tag="PKM49_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNY83165.1"
                     /translation="MTGSKKRLNLLVAFLLFASVALFAKSTGGAEPIPLWEKELSRES
                     IRIVVISDLHLGVDDSFSETVKNKEPMAEFLERLVVSDIDELVIAGDMLDEWFVPITY
                     PPHNDLGIFFRQVADNNSAIIAAFGKLIQSGVKVVYVPGNHDLLLDEETLTGLIPGIS
                     QARDGDGLGVYRAGMRSEIVIEHGHRYDTFCAPDPLSNKEITGDYPSFLPPGYFFTRM
                     AATYVAEGRTAPVKKLPQIEEPSKENGDQQDAYVYYRLWSWAMNSFPIKAGFDEKVFY
                     CGVNGYDDSFSLSDLLPVVQEDGSISAVLYANVQRRWDEVQEICGIAAKNTYALATAG
                     AIDHNFLDNQAVTQYFNLDPTVDVVVFGHSHVPVVNRFQKGYDKEKVYVNSGTWIDGK
                     VGQERCFAVIESGEQTTTVRLMEYHNDGTISLTGE"
BASE COUNT          579 a          561 c          618 g          547 t
ORIGIN      
        1 tttgagaaag tggcttttat cgccgtttgt agcactttta gtcgaaagtt gggttgggaa
       61 cgcctgggaa cgccaacttt taagtcggca tgtttaccgg acgccacaca tcagtttggt
      121 ggtataatcg ccaggttaga ttattagaaa tgcttagaat actttatata gaacaaacga
      181 agtgtatcga taaatcgaaa ggagcgatct aatgacaggt tcaaagaaac ggctcaatct
      241 tttagttgca ttcttgcttt ttgcatcggt agcccttttt gccaaatcga cgggaggagc
      301 cgaaccgata cctttatggg aaaaagagct ttctcgtgaa agcataagaa ttgttgtgat
      361 cagcgacctc catctgggcg tggatgacag tttttctgaa acggtgaaga ataaggaacc
      421 gatggcagaa ttcttggaac ggcttgtagt gagtgatatc gacgaactcg tgatcgccgg
      481 cgatatgctt gacgagtggt tcgtaccgat aacttatccg ccgcacaacg atttgggcat
      541 attcttccgc caagtcgcag ataataactc cgctatcatt gcagcctttg gaaaattgat
      601 ccaaagcggg gtcaaggtcg tctacgtgcc ggggaatcac gaccttctcc tggacgaaga
      661 gacgctaacg ggtttaatcc cagggatttc ccaagcgcgc gatggcgacg gtctgggcgt
      721 ttatcgcgcg ggtatgcgct cggagatcgt catcgagcac gggcaccgat atgacacgtt
      781 ctgcgcgccc gatcctcttt cgaacaaaga aataaccggc gactaccctt catttttacc
      841 tcctggctat tttttcaccc gtatggccgc gacatacgtc gcagagggaa gaacggcgcc
      901 ggtaaaaaag ctccctcaaa tagaagagcc ctcgaaagaa aacggagatc agcaggacgc
      961 ctatgtgtat taccgtttgt ggtcctgggc aatgaacagt ttccccatta aagccggttt
     1021 cgatgaaaaa gttttctact gcggtgtcaa tgggtatgac gacagttttt ccctgagcga
     1081 cttgctcccc gtggttcaag aggacgggtc gatcagcgcc gttctctacg cgaatgtcca
     1141 gaggcgatgg gatgaagtac aggagatctg cggtattgcc gcgaaaaaca cttatgccct
     1201 ggcaaccgcc ggcgctattg accacaactt tttagacaac caagccgtca cgcaatattt
     1261 caacctggac ccgactgtag acgtggtcgt tttcgggcat tcgcacgttc ccgtcgtcaa
     1321 tcgctttcaa aaaggctatg acaaagaaaa agtgtacgtc aacagcggca cctggatcga
     1381 cggaaaagtc ggccaagaga gatgcttcgc ggtcatcgaa tccggtgaac agacgacaac
     1441 cgttcgcttg atggagtatc acaacgacgg aacgataagc cttacgggag aatagtaaac
     1501 ctactaataa atcaaaagac gagcttcctt cgtttctttc tgggagcgcc ggacttccgt
     1561 gcggcatagt ttaagggata aaacagggtt tcgcacagag aacacagagg gcacagagga
     1621 aagattagag attcgctggg aacgcctttc tggacgccat aggtacgccg cactttagtg
     1681 tggcaggtgt caagttctct tcgcgccctc cgtgcgaaac cttttttcga catatgccaa
     1741 cttaaaagtc ggcattccaa ggcggtccca agcgcggtat cgcggcgtaa gcgacccttc
     1801 ttttcgcgac ggcgcgatac cctttttata aggcaaaggg cggatcgcga atttctgtgg
     1861 actgggaacg ccggacttca gtccggcacg gttaaaacca aaacaggggt tcgcacagag
     1921 aacacagagg gcacagagga aagattagag attcgctggg aacgcctggg tgcgccaggc
     1981 ttttgagagc cccctgggaa cgccacactt tagtgtggca tgggttcatt gttctctccg
     2041 tgccctcctt acgaaacttt tttcaacata tgccaactta caagttggcg gtcccagggc
     2101 ggtcccaagc gcgggatcgc ggcgtaagcg ccccttcttt tcgcgacggc gcgataccct
     2161 ttttataaga caaagggcgg atcgcgagtt tctgtggact gggaacgccg gacttcagct
     2221 tggtgggtct tttctttttc tgggagcgcc ctgggaacgc cggacttcag tccggcacgg
     2281 ttaaaaccaa aacaggggtt cgcac
//
LOCUS       DAOPXN010000265         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_51529_length_2243_cov_5.143053, whole genome shotgun sequence.
ACCESSION   DAOPXN010000265 DAOPXN010000000
VERSION     DAOPXN010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51529_length_2243_cov_5.143053"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="PKM49_09995"
     CDS             <1..249
                     /locus_tag="PKM49_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HNY83166.1"
                     /translation="SWMALVFGFGGLRVNEGKLRFHPTVPPAWSGYAFRLYYRRAILE
                     VRARFSAVTYELVEGEGIELVHREMPVSLTSENPKRSL"
     gene            complement(314..1948)
                     /locus_tag="PKM49_10000"
     CDS             complement(314..1948)
                     /locus_tag="PKM49_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HNY83167.1"
                     /translation="MEHYGILGLLPPLITIVLALLSKDVILSLFLGIFSGTLILAGGN
                     PIIAIMKLTDGIANSLADGWNIRILLFCALLGALIGLLAKTGAARAFGLWASSKVKTK
                     TGVLLLTWVFGLLIFIDDYFNSLTIGTVMKPVSDEKKVSRAKLAYILDSTAAPVCILA
                     PISSWVVTVMSLVRDSDGFGSLGVSEFSFFIQSIPFNLYAILTLVMVALIAVTKRDFG
                     PMLRSELRAEKGKGLYDEKTYGPVSGNVEEEVGKLASKPMDMLFPIIVLIVLAIVFFP
                     VTTYMSAIDGETITTFGQAVGSMTLGEAFNNTDASVALFYAVVATLFITYVYFAIRKL
                     LNIKTAADGIVDGIKSMVPAIVILAMAWTIGSLIKNAPADDGLGLSTYLSEVVVNGGF
                     PLALLPLIVFALSCLISFSTGTSWGTFAIMIPITMPIAVSLAQNLGMTGAALMNAALI
                     SVGGVLAGAIFGDHCSPISDTTILSSIGASCPHLEHVATQIPYAVFIAACAGVGFLFA
                     GLTNNMIFGWAAALVIFIGGMFLLPKVLGANRYSLD"
BASE COUNT          566 a          590 c          602 g          485 t
ORIGIN      
        1 tcttggatgg ctctggtttt cggcttcggt gggttgcgag tcaacgaagg caaacttcgc
       61 tttcacccga ccgttcctcc agcttggagc ggatatgcgt tcagattgta ctaccgtcgc
      121 gccatactgg aagtccgcgc gcgcttttca gcggtgacct atgagctggt cgagggagaa
      181 ggtatcgaac tcgttcaccg agagatgccg gtttccctaa cgagcgagaa tccgaagcgt
      241 tctctatgaa ataagaatag aggcgccgag gcgcctctat tctatgtcgt actcttatga
      301 atgtcctaac gcgttagtcc agcgaatagc gattggcgcc aagaaccttt ggcagtaaga
      361 acatcccgcc gataaaaatg accagtgcgg cggcccaacc gaaaatcata ttgttcgtca
      421 gccccgcgaa caggaagccc acacctgcgc aggcggcgat gaacacggca taggggattt
      481 gcgtggctac gtgttcgaga tgggggcaag aggcgccgat agaggacaga atcgtcgtgt
      541 cggagatcgg ggaacagtga tcaccgaaga tcgctcccgc cagaacgcct ccgacgctga
      601 tcaacgccgc gttcatcaac gcagctcctg tcattccgag gttttgggct aaggatacgg
      661 cgatcggcat agtgataggt atcatgatcg cgaaggttcc ccagctggta ccggtggaga
      721 aggagataag gcaagagagg gcgaagacga tcaacgggag caacgccaag gggaatcctc
      781 cattcacgac gacttcggac agataggtgg aaaggcctaa accgtcatcc gccggagcat
      841 ttttaattag gctaccgatc gtccaagcca tcgccaaaat aacgatcgcc ggcaccatcg
      901 atttgatacc gtctacgatg ccatcggcgg ccgttttgat gttcaaaagt ttgcggatgg
      961 cgaaatagac gtacgtgatg aacaaggtgg cgacaacggc gtagaaaagg gcgacggaag
     1021 cgtccgtgtt gttaaaggct tccccgaggg tcatcgaccc gacggcctgt ccaaaggtcg
     1081 tgatcgtttc gccgtcgatc gcgctcatat acgtcgtgac cgggaagaag actatggcca
     1141 acacgatcaa cacgatgata ggaaagagca tatccatcgg tttggaggcg agttttccca
     1201 cctcttcttc cacgttcccc gagacgggtc cgtacgtctt ttcgtcgtag agccccttcc
     1261 ctttttccgc acgcagctcg ctacgcagca taggcccgaa atcgcgcttg gtgacggcga
     1321 tgagggccac catcaccaat gtcaaaatcg cgtacaggtt aaacggaatc gattggatga
     1381 agaacgagaa ttccgagacc cctaatgagc cgaatccgtc ggaatctcgg accaagctca
     1441 tcacggtgac aacccagctg gaaatggggg ctaagataca cactggcgcg gcggtcgaat
     1501 cgaggatata agccaacttc gcgcgcgaca cctttttttc gtcggatacc ggtttcatca
     1561 cggttccgat ggtcaaggag ttgaagtagt cgtcgatgaa gatcagaagt ccgaaaaccc
     1621 acgtcaaaag caagactccg gttttcgttt tgaccttcga cgaggcccat agcccaaacg
     1681 ctcgcgccgc cccggtcttc gccaacagcc cgatcaaggc gcccaacaac gcgcagaaca
     1741 acaggatgcg gatgttccat ccatcggcta aagaattcgc gataccatcc gtgagtttca
     1801 tgattgcgat gatcggattt ccgcccgcca agatcagggt tccagagaag atgcccaaga
     1861 acagagaaag gatgacatct ttgcttagaa gcgccaatac gatcgtgatc aaaggcggca
     1921 gaagtcccag aatgccataa tgctccatct tacgtacccc cttatagtat gaagtgcact
     1981 gactcttaac ccctgtattc ttaaaaatga gactcggcga attataccat tttttacgtt
     2041 tttctatatc caaaacgtgt gggaataata tcgaggcgtt cccagggggt tcacaagaat
     2101 cttttctccg tgccctctgt gccctcagtg tgaccctgtt tttcaaaaca tgtataaaag
     2161 cgtatcgcgc agttgctgaa agaagaagcg cttacgccgc gatggtgcgc ctgggaacgc
     2221 cgaactttag ttcggcatgt ttt
//
LOCUS       DAOPXN010000266         2203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_52968_length_2203_cov_4.651769, whole genome shotgun sequence.
ACCESSION   DAOPXN010000266 DAOPXN010000000
VERSION     DAOPXN010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52968_length_2203_cov_4.651769"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1899)
                     /locus_tag="PKM49_10005"
     CDS             complement(277..1899)
                     /locus_tag="PKM49_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83168.1"
                     /translation="MRQRWLKVLIGIGLAALGFALVELILTSRLDLTIDFSFYPELDR
                     NTPFKVSIKSGAVQQQHMVSPASPRLTLKGIKIDPYQLTVSLDKMDIVSTELNFGRSF
                     DFYRKKERVEISVTELSTVAFLETTIDDPLLRIKWQVRNLKDYVPSGFEVSVQGVWQR
                     SSVNFLEVDLRKLLKNPEDTILFGLSPVSQEGYRLTTFEKTLSARLANMKITLPPQIN
                     PFEISVQIGGRIIQMDPYTNLVSFPYLADVDETPVDILYYQQPILSLALPPEKMNQTL
                     VVPPVPAATVSQIEVLDNGLRLHFALDNPPESEGFLADHFRYFSVQGTSFEATASKEF
                     FLPATQTAAELFIVPEFDYGIQGRAARFIKPEKPVVKVALTSQKSAGALHAKILTSTA
                     APLTGRYKVDRQDWVTIPSFERETEIDIPMTFDQIHSLEVAVKDAYGQTGEVKKWVDP
                     STPQTTFFKYVKVEGTVLKAEWEPLTVYDEVTVTITDNYNRIAFVTKASSFETELSRS
                     GLFFPLKIVIKGKLSEDTYTAVIEENILPPAK"
     gene            complement(1981..>2203)
                     /locus_tag="PKM49_10010"
     CDS             complement(1981..>2203)
                     /locus_tag="PKM49_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HNY83169.1"
                     /translation="AEGHGKAIVTVFNAIHEGKATNDVIAVRYLETLKEVANGQATKI
                     FLPAEVSGILGSLGGIGELFKKEEDSGKK"
BASE COUNT          468 a          589 c          607 g          539 t
ORIGIN      
        1 aagacatgcc ggactaaagt ccggcgttcc caggcggccc agggcggatc gcgagacttc
       61 gtgggcttcc gatgtcagcc cgttggttac cccgcgataa cttgcgcggg catcgcggcg
      121 taagcgctcc tttttcacgc gacggcgcga taccccgacg tcatcgcggc gcaaaaagtg
      181 taaaaggggg atcgcggggt cgtaaaaacc acttaccccg cgataacccc ataaaattcg
      241 gcggtcccag atgggcggtc ccaggccggt acggatttat ttcgccggcg gaaggatgtt
      301 ttcttcgatc acggccgtgt aggtgtcttc tgagagcttg cctttgataa cgatttttaa
      361 ggggaagaac aagccggagc ggctgagttc agtttcgaag gaggaggctt tcgtcacgaa
      421 ggcgatacgg ttgtaattat cagtgatcgt gaccgtgacc tcgtcgtaga cggtcaacgg
      481 ttcccattcg gctttcaaga cggtcccctc gactttcacg tatttgaaaa aggtggtctg
      541 cggcgtcgag gggtcgaccc atttcttgac ctcccccgtt tggccgtagg catccttcac
      601 cgcgacctcc aaagagtgta tctgatcgaa ggtcatcggt atgtcgattt ctgtttccct
      661 ttcgaaggac ggaatcgtca cccaatcttg ccggtccact ttgtaccgac cggtcagagg
      721 agccgcggtc gaggtgagta tcttcgcgtg gagggcgccg gcacttttct ggctggtcaa
      781 cgccactttc acgaccggtt tttccggttt gatgaaccgc gcggctctcc cctgtatccc
      841 gtaatcaaat tcgggaacga taaagagttc ggcggccgtc tgagtcgccg gtaaaaaaaa
      901 ctctttcgat gcggtggctt caaagctcgt tccctgtaca gaaaaatacc gaaagtgatc
      961 ggctaagaag ccctcggatt cgggggggtt gtccagagcg aagtggagtc gaaggccgtt
     1021 gtcgaggacc tcgatctgcg agaccgtcgc cgccggaacc ggcgggacga cgagcgtctg
     1081 attcatcttt tccggaggta gcgcaaggct cagaatcggc tgctgatagt aaaggatatc
     1141 taccggggtt tcgtccacgt ccgccaagta gggaaaagag acgagattgg tgtacggatc
     1201 catctggatg atccgccctc ctatctggac cgagatctca aagggattga tctgcggcgg
     1261 taaggtgatt ttcatattgg caagacgggc ggataacgtt ttttcgaagg tggtcaaccg
     1321 atagccttct tgagaaaccg gcgataagcc gaaaaggatc gtgtcttccg ggttttttag
     1381 tagttttcgc agatcaacct cgaggaagtt cacgctcgat ctttgccaga cgccctgcac
     1441 gctgacttca aagcccgaag gcacgtagtc cttcaggttt ctgacctgcc attttatccg
     1501 caacagcgga tcgtcgatcg tggtttccag aaaagcgacg gtagaaagtt cagtcacgga
     1561 gatctccaca cgctcttttt tccgatagaa gtcgaaggag cgcccgaagt tcaactccgt
     1621 ggatacgatg tccattttgt ccaatgacac cgtcaattgg taggggtcta ttttgatccc
     1681 cttcaacgtg agacgtggcg atgcgggtga taccatatgt tgctgttgaa cggctccgct
     1741 tttaatcgaa accttgaaag gggtgttcct gtccagctcg ggatagaacg agaagtcaat
     1801 cgtgagatcc aggcggctcg tcaagattag ctcgaccaat gcgaatccga gcgcggccaa
     1861 acctattccg atcagaacct tcaaccatcg ttgtctcaag aaaacacccc tcgcctgacg
     1921 tttctcccgg ttgctcggaa gaagtctatc gcgggcttcc caccgttctc tgaggacgct
     1981 ctatttcttt ccggaatcct cttctttttt gaatagctct ccgataccac ccaagctgcc
     2041 gaggataccg ctgacctccg ccggtaggaa gattttcgtg gcctgtccgt ttgccacctc
     2101 tttcaacgtt tctaaatatc ggaccgctat gacatcgttc gtggcctttc cttcgtggat
     2161 ggcgttgaag acggtgacga tcgctttccc gtgcccctcc gct
//
LOCUS       DAOPXN010000267         2193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_53349_length_2193_cov_4.216090, whole genome shotgun sequence.
ACCESSION   DAOPXN010000267 DAOPXN010000000
VERSION     DAOPXN010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53349_length_2193_cov_4.216090"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..2021)
                     /locus_tag="PKM49_10015"
     CDS             complement(291..2021)
                     /locus_tag="PKM49_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012003383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HNY83170.1"
                     /translation="MSSINLTVDGKLYQVPAGLTVMEACRKVGIKIPSLCYHPRLEST
                     GACRICAIEVDGFRNLQAACVTLVSDGMQIKTHTSRVINARKMNLALLLSNHPNKCMT
                     CESNGSCEFQDLIYDYGIEEELPKMPHDPGLIDDSSLAITRDLNKCVSCGRCIRACSD
                     LQAMDIYSMAYRGVNSLPLTAFDRGLSDTLCISCGQCSLFCPVGAITENPSLRRLREA
                     MESGEYVMVVQTAPATRVAISEELGLPPGTISTGRMVAALRKLGFDYVFDTDFSADLT
                     IMEEGSELLTRIKEGGKFPMFTSCCPAWINLVEKEFPEYMPNLSTAKSPQQMMGAVVK
                     TYFADKIGVSPDKIFLVSIMPCTAKKEEALRPQQLMSSGAPYVDLVLTTRELGRRIRL
                     SKLQVEHLEDSRYDDPLGASTGAAVIFGATGGVMEAALRTAYELATGKPLPRLGFEEV
                     RGIQGIKEATVALDDKTLRVAVAHGGANVRKLLTAIKNGDVYYDFVEMMACPGGCIGG
                     GGEPYSDDPDIIEKRMRGIYRADELNTIRKSHENPSIKAIYEDFFETPLSHRSHELLH
                     THYTDRSNKN"
BASE COUNT          506 a          583 c          548 g          556 t
ORIGIN      
        1 tttgagaaag tggcttttat cgccgtttgt agcactttta gtcgaaagtt gggtcgggac
       61 gttcacaagc gtatcgcgcc gtcgcgaaaa gaaggggcgc ttacgccgcg atgctgcgct
      121 tgcgaacgtt cccaaggtgt tcccagagcg ttctcgggac attcacaagc gtatcgcgcc
      181 gtcgcgagaa gaagtggcgc ttacgccgcg atgctgcgct cgggaacgct accaaggcgt
      241 tcccagacgt tcccagtgaa cactccggga cgccccgtga tttaaaaagc tcaattcttg
      301 tttgagcgat ccgtgtaatg cgtatggagc agttcgtgtg accgatggct tagcggagtc
      361 tcgaaaaaat cttcgtatat ggcctttatc gaaggatttt cgtgagactt tcttatcgta
      421 ttcaactcat cagccctata gatcccacgc atccgttttt cgataatgtc ggggtcatcg
      481 ctataaggct cgccgcctcc gccgatgcac cctcctgggc aggccatcat ctcgacaaaa
      541 tcgtaataga catcgccgtt tttaatcgct gtcagtagct ttcggacgtt cgcgcctccg
      601 tgcgcaacgg caacccttaa ggttttatcg tctagcgcca ccgtggcctc tttaatgcct
      661 tgaatgccac gaacttcctc aaaacccagt cgaggtaaag gctttcccgt tgccagttcg
      721 tatgccgttc gtagggccgc ttccataacg cctccggtcg ccccgaagat gactgccgcg
      781 ccggtggaag cgcctaaggg atcatcgtat cgtgagtctt ccaagtgttc gacctgtaat
      841 ttggacaacc gaatccggcg ccctaactct cgcgtggtta acacgaggtc gacgtagggc
      901 gctccagagc tcattaattg ctgaggtcga agcgcctctt cctttttggc cgtacagggc
      961 atgatcgata ccaagaatat cttatcggga gaaacgccga ttttatcggc gaaatatgtc
     1021 ttcaccactg ctcccatcat ttgttgggga gatttcgccg ttgagagatt tggcatatat
     1081 tccgggaact ctttttccac gaggtttatc caagcgggac agcaggaggt gaacatcgga
     1141 aatttcccgc cttctttgat ccgggtcaga agctcggaac cctcttccat aattgtgagg
     1201 tcggcgctga agtccgtatc gaatacgtag tcgaagccca atttccttaa agccgcaacc
     1261 attcttcctg tagagatggt tccgggggga aggcccagtt cttcactgat tgccactcga
     1321 gtcgcgggcg ccgtctgtac aaccatcacg tattcgccac tttccatcgc ctcgcgtagt
     1381 ctgcgtagag aaggattttc ggtgattgcg cccaccgggc aaaataggga gcactgcccg
     1441 cagctgatgc acagggtatc cgatagaccc ctgtcgaaag cggtcaaagg caaggaattc
     1501 actccccgat aggccataga ataaatatcc atcgcctgga gatccgaaca cgcgcgaata
     1561 cacctgccgc aagagacgca tttgttcaaa tctctcgtaa tggctaagga actatcgtct
     1621 atcagccctg ggtcgtgggg catttttgga agctcttctt cgatcccgta atcgtagatc
     1681 agatcctgaa attcgcaaga accgttcgac tcgcacgtca tacatttgtt tggatgattg
     1741 gaaaggagca aagcgagatt catctttctc gcgttaatca cacgcgaagt gtgcgttttg
     1801 atttgcattc cgtcggagac caaggtgacg cacgcggcct gaagattacg aaatccatcg
     1861 acttcgatgg cgcagatcct gcacgcgccc gtactctcca aacgaggatg gtagcataga
     1921 ctggggattt ttattcccac cttccgacaa gcttccatca cagtgagccc agccggcacc
     1981 tgatagagtt ttccgtccac agtcaaatta atcgacgaca aaattcctcc tttttcgtaa
     2041 gcgcctgcac ctctagcaga cattggaatg aacgtttttc aagaaaacta ttataacata
     2101 atgagctccg tgagttgatc aacggtgtgg ggataatatc ggtgtacaca aaatgggaac
     2161 gccgaacttt agttcggcac gtttttttgc ttt
//
LOCUS       DAOPXN010000268         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_54567_length_2160_cov_6.067933, whole genome shotgun sequence.
ACCESSION   DAOPXN010000268 DAOPXN010000000
VERSION     DAOPXN010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54567_length_2160_cov_6.067933"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            532..1419
                     /locus_tag="PKM49_10020"
     CDS             532..1419
                     /locus_tag="PKM49_10020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HNY83171.1"
                     /translation="MEKKYLNPLLDYTFKKIFGNEKHKEILIHFLNSLGIESQGHKIV
                     DLRILDHTNEKRFKLDKYSVMDIKATLNNGELVNIEVQILPYEHMLQRTIYYLTKMYS
                     DQLTQGDDYGKLCNTITVNVLGYNQFEHDRIHSVYHIRDAETCEKLGDFLEIHFIETQ
                     KIGKSPQKLGEDLKGWLLFLSNPENQRITELGKEVGEVGQAINLLEELSRSREERAIA
                     EEREKAIRDELSFRKAELEKGLQKGLEKGLQKGLKIGREEEKYEIAKKLIHKGMDLSE
                     VLEITGLSLKDLKNIGNLT"
     gene            complement(1867..>2160)
                     /locus_tag="PKM49_10025"
     CDS             complement(1867..>2160)
                     /locus_tag="PKM49_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83172.1"
                     /translation="LTIKPKTILERETVKIGLRNREIDLIVKGNIELWIDVKDTKGKY
                     GKREADRWIEIKQAITEKNPKTLFATFSQNGYTSSAKRLLVSNGVYVLKGEEG"
BASE COUNT          578 a          543 c          523 g          516 t
ORIGIN      
        1 cgccgcgatg cgcccacgca ggaacacctg agaacaccct gggaacgccg gacttcgagg
       61 atcatcgcgg ggtaagtggt ttttacgacc ccgcgatccg ctctttctcc ccctcaaaag
      121 cgtatcgcgc cgtcgcgaaa agaagggtcg cttacgccgc gatgcgccca cgcaggaaca
      181 cctgagaaca ccctgggaac gccggacttt agtccggcac gttcttaagt tttccgcact
      241 cctccgtgct ctctgtgccc tctgtgcgaa ccctgttttt caagacatgt agaaaagcgt
      301 atcgcgccgt cgcgagaaga agggacgctt acgccgcgat gcttgttttc accgtggcgt
      361 ctgtgcactc cgcgtgcaat ctcactcttt acccttccaa aaagacatgc caactcaaaa
      421 agcatgcgtt cccagaaaac cattagtgga tatcaaagaa tactcgaaac gagaactacg
      481 tccgaaaaag tggtatgctt aacacaaacg gtcactatgg aggtttcccc gatggagaaa
      541 aagtatctga atccactgct cgactatacc ttcaagaaga ttttcggaaa tgaaaaacac
      601 aaagagatcc tgatccattt cttaaactcc ttagggatcg aaagccaagg gcataagatc
      661 gtcgacctac gcatactcga ccatacgaat gaaaaacggt ttaagctgga caagtattcg
      721 gtaatggaca tcaaggcgac gttgaacaac ggggaactcg tcaacatcga ggtacagata
      781 ctcccgtacg aacatatgct ccagaggacg atatactatt tgacaaaaat gtattcggac
      841 cagctgacgc aaggtgacga ttacggcaag ttatgcaaca cgatcacagt gaatgttttg
      901 gggtacaatc aattcgagca cgacaggatc cactctgtct atcatatacg ggacgcagaa
      961 acgtgtgaaa agctggggga ctttctggag atacacttta tcgagacgca gaagattggg
     1021 aagagtccac aaaaattagg agaagacctc aagggttggc tattgttctt gagtaaccct
     1081 gaaaaccaaa ggataacgga gctcgggaag gaggtaggag aggtggggca agcgatcaac
     1141 ctgttagaag aactgagccg gagtcgagaa gagcgagcga tagcggaaga gcgcgagaaa
     1201 gcgattcgcg acgaactttc ctttcggaaa gccgaattgg aaaaaggatt acaaaaagga
     1261 ttagaaaaag gattacaaaa aggattaaag ataggccgag aggaagagaa atacgaaatc
     1321 gccaaaaagc tcatacacaa ggggatggat ttgtcagagg tcttggagat aactggacta
     1381 tctctcaagg acttaaagaa tatcggtaac ctcacgtaag tcaaggataa gccgctttcc
     1441 ggaagagccg ccggagttcc cgggatcatc tgggagcgcc taacttcgtg ggccatcgcg
     1501 gggtaagtgg tttctacgac cccgcgatgc cgcccacgaa ggaacacctg agaacgccat
     1561 gggaacgccg cactttagtg cggcatgtct tctacgtttt catcctctct ccgtgccctc
     1621 tgtgttctct gtgcgaaacc attctttatc ttttttcggc ctatataccg tgccaactta
     1681 aaagttggcg ttcccagaga gacaggccaa ctggaagtcg gccccacgag ttttcttcag
     1741 ccttttctct ctgttctctg tgccctctgt gctctccgtg cgaaaccatc ctttatcttt
     1801 ttttggctac aatacacgcc aacttaaaag ttcggcattc tcaggcgttt ccaattgcgc
     1861 ccattattac ccttcttccc ccttaagcac atacactccg ttcgatacga ggagcctttt
     1921 cgcggaagaa gtgtacccgt tttgggagaa cgtcgcgaat agggttttcg gatttttttc
     1981 ggtaattgcc tgcttgatct ctatccagcg gtccgcttcg cgtttcccat acttcccttt
     2041 cgtgtctttc acgtcgatcc acaactcgat gttgcctttc acgattagat cgatctctcg
     2101 gttccgtaac ccgattttga ccgtttctcg ctctaagatc gttttcggtt tgatggtgag
//
LOCUS       DAOPXN010000269         2154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_54809_length_2154_cov_5.918533, whole genome shotgun sequence.
ACCESSION   DAOPXN010000269 DAOPXN010000000
VERSION     DAOPXN010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54809_length_2154_cov_5.918533"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKM49_10030"
     CDS             <1..227
                     /locus_tag="PKM49_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83173.1"
                     /translation="LIVKGNVEMWIDVKDTKGKYGKREADRWIEIKNIINDKSPKTLF
                     ATYSQNGYTASAKNILVSNGVYVLKGEEGE"
     gene            complement(651..1865)
                     /locus_tag="PKM49_10035"
     CDS             complement(651..1865)
                     /locus_tag="PKM49_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008795619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNY83174.1"
                     /translation="MARIERKEYLDWLIRWSGQRVIKVVSGVRRCGKSTLLEMFREYL
                     LEQGVNPQQILSFNFEDIELEHLTEYRALYQAIQSALSPDKMNYIFLDEIQHVSRFEK
                     VIDSLFLKSNCDIYITGSNAYLLSGELATLLSGRYVELKMLPLSFDEFCRGVPDSVRR
                     LPKTEKFNRYVEIGSFPFIAQFADKEREAKEYLRDIYNSVLLKDVVTRLKIADIQNLE
                     NLSKFLFHNIGNRASVRKIADTLKSQGKGVDQKTVEKYMRGLIDGLLLYEARRYNIKG
                     RQHLTTQSKYYAVDIGLRNLLVRGQEADHGYVLENLVYLELIRRGYETFVGQLDDGEV
                     DFVTKTPDGTAYFQVATSALNENVLQRELFPLRKIVDNYPKYLLTLDEAFASADYQGI
                     RKMNVIDWLLGD"
BASE COUNT          572 a          558 c          505 g          519 t
ORIGIN      
        1 acctgatcgt gaaaggcaac gtcgaaatgt ggatcgacgt gaaagacacg aaaggcaagt
       61 acggaaagcg agaagcggac cgctggatag agatcaaaaa cataatcaac gataaaagtc
      121 caaaaacctt attcgcaact tactctcaaa acgggtacac ggcttccgcg aaaaacatac
      181 tggtatcgaa tggggtatat gtgcttaagg gtgaagaggg ggaataggag caccctggga
      241 acgccgaact ttagttcggc atgttttttg gaaaaacaaa agaagtttcg cactgagtac
      301 acggtgaaca cagagagaac acagagagaa cacagagaaa tgccctggcc cccctgggaa
      361 caccgaactt aataggtaat cgcggggtaa ctaacaggac cccgcgatcc gctctatgtc
      421 tttctcaaaa gcgtatcgcg ccgtcgcgaa aagaagaagc gcttacgccg cgatgctgcg
      481 cttgggaacg cttcccacct ccgtgccctc tgtgccctct gtgcgaaccc tgtttttaag
      541 gcatgccaac ttaaaagttg gtgttcccaa gagtgcgttc ccaaacccat ggcgaactaa
      601 agttcggggc tcccagaaga gaagcgttcc cagaatgcac gttggcccga tcagtctcca
      661 agcagccaat cgataacatt catctttcgg atgccttggt aatcggcgct cgcgaaggcc
      721 tcgtctaagg ttaagaggta tttcgggtag ttgtcgacga tttttctgag cgggaaaagc
      781 tctctttgca agacgttttc attcaacgcg cttgtggcga cttgaaaata cgccgttcca
      841 tccggggtct ttgtcacgaa atcgacttcg ccgtcatcca gttgcccgac aaacgtctca
      901 tatccacgcc ggatcagttc cagatagaca aggttctcca agacataacc gtgatccgct
      961 tcctgacctc tgactaagag gtttctcagt ccaatatcca cggcgtaata tttactttgc
     1021 gttgtcaaat gctgccgtcc tttgatgttg taccgccgcg cttcgtagag cagcaaaccg
     1081 tctatcagtc cgcgcatata cttctcaacg gttttctgat ccactccttt cccttgggat
     1141 ttcaaggtat cggcaatttt cctcacggag gccctgttgc cgatattgtg gaacaaaaac
     1201 ttcgaaaggt tctcgaggtt ttgtatatcc gcgattttta acctcgtgac cacatctttg
     1261 agcaatacgg agttgtaaat gtccctcaga tactcttttg cctcccgctc tttgtccgcg
     1321 aactgggcga taaacgggaa agagccgatt tccacgtacc ggttgaactt ttcggttttc
     1381 ggcagccggc gaaccgaatc gggcaccccc ctgcaaaact catcgaagct caagggcagc
     1441 atcttcagct cgacatatcg accggataaa agcgtcgcca gctcccccga tagtaaatag
     1501 gcgttggaac cggtgatata gatgtcacag ttgctcttca ggaaaaggct atcgatgact
     1561 ttttcgaatc gactgacgtg ttgtatctca tcgagaaaaa tatagttcat cttatccggc
     1621 gacagcgccg attggattgc ctgatacaac gcccgatact ccgtgaggtg ttcaagttcg
     1681 atgtcctcga agttaaacga cagaatctgc tgtgggttca ccccttgctc gagaaggtat
     1741 tcgcgaaaca tctccagcaa cgtcgacttc ccgcagcgcc gtaccccgga tacgactttg
     1801 atgacccgtt gaccactcca gcgtatcaac caatccaggt attccttccg ctctatgcgc
     1861 gccatttcct ccccccttct gacattattg ggagtatact ccatattttt tcagaaattg
     1921 gtatttttga ggaatgcatt cctcaaaaaa gaagtcgcgc acagagtaca cagggtatcg
     1981 cggggtaggt gatttttacg accccgcgat ccgctctatg tctttccaaa accaaaagaa
     2041 gtttcgcact gagtacacag agatcacaga gagaacacag agaaatgtcc tgggaacccc
     2101 ctagcccgac tttcgaccgg ttttggagaa aaagggaaaa aataagaaaa aaca
//
LOCUS       DAOPXN010000270         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_57018_length_2096_cov_5.120039, whole genome shotgun sequence.
ACCESSION   DAOPXN010000270 DAOPXN010000000
VERSION     DAOPXN010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57018_length_2096_cov_5.120039"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PKM49_10040"
     CDS             complement(<1..347)
                     /locus_tag="PKM49_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83175.1"
                     /translation="MGVSTRRENLKELASIRTSKDLNYLRSLFPYDPESGVFTIEITI
                     DHYDEIFNEWDPSPFRRRDLHPDLTDYLDYCSKEIPLKYPIRLSIEVPEEKRNATAEK
                     MVEQGIRNNIRMD"
     gene            complement(317..862)
                     /gene="tsaE"
                     /locus_tag="PKM49_10045"
     CDS             complement(317..862)
                     /gene="tsaE"
                     /locus_tag="PKM49_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HNY83176.1"
                     /translation="MGDGSVRLSPKGSGGRKTIRELEKSGHAIARLGLRLESFYRHID
                     EGELRDLARAYAARIRKGETIFLSGELGSGKTTFMKAVAEAYGYDPSAVRSPTFTLIN
                     RYETKETVLFHLDCFRIDAETLSNIGFFDLPTDEARVFIEWPENVLPVFQKADWMFRF
                     QIPEDTLTVRDLWVYRHDEKT"
     gene            complement(754..>2096)
                     /gene="glpK"
                     /locus_tag="PKM49_10050"
     CDS             complement(754..>2096)
                     /gene="glpK"
                     /locus_tag="PKM49_10050"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNY83177.1"
                     /translation="VEQDPEEILATAQATIAQAMEKAGLRETDIAAIGITNQRETTIA
                     WNRKTGESLHPAIVWQDRRTAERCEALLPEQETLHTLTGLVLDPYFSATKMEWLLRHV
                     PKVKIAAENGVLSFGTVDSWLAYHLTDRKAYVTDVSNASRTMLLNLRTTAWDDTCLSL
                     FRLRREMLPTVVTSSEIVGHTPSGIPIASMIGDQQGALFGQMCFEPGQAKCTYGTGAF
                     LLMNVGTEPKFSANRLLTTIAWKAGNELAYAMEGSVFICGASIDWIVNELGLAPNAAS
                     TEQMAYNVRDNGGVYFVPALSGLGAPYWDPQARGLWIGMTQATNRNHLTRSVLEGIAY
                     SVRDLSDTIVTDSGIPLKELKIDGGVSKNTFLQNYQSTVLGLPVLRPKNTETTATGAA
                     CLAGLAVGFWKDRSELRGLWEMDRYVYRQKEAEVERQYANWKKAVTRSLDWVYD"
BASE COUNT          421 a          593 c          584 g          498 t
ORIGIN      
        1 atatccatac ggatgttgtt gcgaattccc tgttccacca tcttttcggc ggtggcgttg
       61 cgcttttcct ccggaacttc aatcgagagt cgaatcgggt acttgagggg tatttctttg
      121 gagcaataat ccaagtagtc ggtcaaatcc gggtgtaggt cccgccgccg aaagggagag
      181 gggtcccatt cgttgaagat ctcgtcgtag tgatcgatgg tgatttcgat cgtgaagaca
      241 ccgctctccg gatcgtaggg gaagagcgat cgtagatagt tcaaatcttt agaagtccgg
      301 atagaggcca actccttcag gttttctcgt cgtgtcgata cacccacagg tcccttaccg
      361 tcagggtgtc ttcggggatt tgaaaacgga acatccaatc tgccttctgg aagacgggca
      421 acacgttctc aggccactct ataaagaccc tggcctcgtc ggtgggaagg tcgaagaatc
      481 cgatatttga tagcgtctcg gcgtcgattc tgaaacaatc caggtggaaa aggacggtct
      541 ctttggtctc gtagcggttg atcagcgtga acgtcgggct tcgcaccgcg ctcgggtcgt
      601 aaccgtaagc ttcggccacc gctttcataa aggtagtctt gccgcttccg agctcgccgg
      661 acaagaatat ggtctctcct tttcttatcc gcgccgcgta ggcgcgcgcg agatcacgca
      721 gttcaccttc gtctatatgc cgatagaacg actctaatcg taaacccagt cgagcgatcg
      781 cgtgaccgct tttttccaat tcgcgtattg tctttctacc tccgcttcct tttggcgata
      841 gacgtaccga tccatctccc ataatccgcg caattcggaa cggtccttcc agaatccgac
      901 tgccaacccc gcgagacacg ccgcgccggt cgcggtggtt tcggtatttt tcgggcgtag
      961 gaccggtagc cccaaaaccg tcgattggta attttgcaaa aacgtgtttt tactgacacc
     1021 gccgtcgatt ttgagctctt tgagcggaat accgctgtcc gttacgatcg tgtcggaaag
     1081 gtcgcgcacg ctgtaagcaa tcccctccaa cacggagcgg gtgaggtgat ttctgtttgt
     1141 cgcttgggtc atcccgatcc acaatccccg ggcctgcggg tcccagtaag gggcgcccag
     1201 gccggacaaa gccggaacga agtagacccc gccgttgtcg cggacgttgt acgccatctg
     1261 ttcggtgctg gccgcgtttg gggccaaacc caactcgttg acgatccaat cgatggaagc
     1321 gccgcagatg aagacgctcc cttccatcgc gtaggcgagc tcgttgcccg ctttccaagc
     1381 gatcgtcgtg agtaggcgat tggcagaaaa cttcggctcg gtccccacat tcatcagtaa
     1441 aaaggcgccg gtcccgtagg tgcacttcgc ttgtcccggc tcgaagcaca tctggccaaa
     1501 taaggcgccc tgttggtctc caatcatcga agcgatcgga atcccgctcg gcgtgtggcc
     1561 gacgatctcg gacgaggtca ccaccgtcgg cagcatctct cgacgcaggc ggaacaaaga
     1621 caggcaagta tcgtcccaag cggtcgtgcg taggttcagc aacatcgtgc gggaggcgtt
     1681 agagacgtcg gtcacatagg cttttctgtc cgttaagtga taggcgagcc aggaatcgac
     1741 cgttccgaaa gacaaaacgc cattttcggc ggcgatcttc accttgggga cgtgcctgag
     1801 taaccattcc attttcgtcg cagagaagta ggggtcgaga acgaggccag tcagagtatg
     1861 tagggtttct tgctcgggca gaagcgcctc gcaccgttcg gccgtcctcc ggtcttgcca
     1921 aacgatcgcg gggtgcaacg actctccggt tttacgattc caagcgatcg tcgtttcacg
     1981 ctgattggtg atcccgattg ccgcgatatc ggtctctcgc agcccggcct tttccattgc
     2041 ttgggctatc gtggcctgag cggtggccaa gatctcttcc gggtcctgct ccaccc
//
LOCUS       DAOPXN010000271         2064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_58344_length_2064_cov_5.761075, whole genome shotgun sequence.
ACCESSION   DAOPXN010000271 DAOPXN010000000
VERSION     DAOPXN010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58344_length_2064_cov_5.761075"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(325..726)
                     /locus_tag="PKM49_10055"
     CDS             complement(325..726)
                     /locus_tag="PKM49_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83178.1"
                     /translation="MKTLQDLFEMAFSFVLKELLDGNLDRADIANKTFDRLVQMGYVE
                     DDIHTTLEVIFSLDEIIEGGIFSSGIHHVVFEVYQPKRRIPVELLLLEEKDADLEVNF
                     RDLYKGNLSPERFELYVEVGEDLDVPDDTPN"
     gene            complement(780..1772)
                     /locus_tag="PKM49_10060"
     CDS             complement(780..1772)
                     /locus_tag="PKM49_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNY83179.1"
                     /translation="MLKTVKWGVLGAARIAEKAVIPAILQTPGCVLEAVASRDREKAR
                     RFFSLFQPAKTYDTYTQLLNDREVDAVYVPLPNALHKEWVTNALLAGKHVLCEKPLAL
                     NSLDAREMFRVARGTGKILMEGFAYIHNPLIEHLRKALQTGEIGEIRHLMTSFSFDLS
                     KRPKDIRWSRELGGGAVYDLGCYLVHLARYLTEKEPTVAHAVASLHPVEGVDLSATAL
                     LRFDRGPTMSFHVSFEQSRRMFFEAQGTRGALYTERPFNETGTLTYTVVKEEGSRTFQ
                     VESPNNYALEIAHFNATIRAEVTPVISEEDSVHNAAILTSILERVGYGTRTASR"
BASE COUNT          464 a          545 c          521 g          534 t
ORIGIN      
        1 tgtgacttac tgattttctc cacgggtatc cctccagaat aaagtctcat cggtaatata
       61 ccgcaaaggt gggatttcca aaaagggaaa gtgaatccac cttttgtcct tttatgtcgt
      121 gatgactaca tggttcattg cggggtaaga ggttttcacg accccgcgat ccgccctctg
      181 ctcttctcaa aagcgtatcg cgccgtcgcg aaaagaagag gcgcttacgc cgcgatgacc
      241 tcgcgggcta aaaatcgtgc gatcgattct ggccttgttt tttgagggaa cctgaaaact
      301 tggcacacac ttgagcggga ttctttaatt cggggtgtcg tcggggacat ccaaatcttc
      361 tccgacttcg acgtacagct cgaagcgctc aggagagaga tttcctttgt ataggtcgcg
      421 gaagttgacc tccaaatccg catctttttc ttcaagtagc agtaattcga cggggattct
      481 ccgcttcggt tggtatactt cgaacacgac gtggtggata ccggaagaaa agatgccgcc
      541 ctctatgatt tcatcgagcg agaaaatgac ttctaacgtg gtatggatgt catcttccac
      601 gtatcccatc tgtaccagcc gatcgaaggt tttatttgcg atatcggcgc gatctagatt
      661 gccgtccagc agctctttca gcacgaacga aaaggccatc tcgaacagat cttgtagagt
      721 cttcaaacaa ccccgtcctt tttaaaaagt ttgcttatta tagcacaatt gcgatcgaat
      781 catcgggagg cggtgcgcgt cccgtatccg accctttcga gtatagacgt caaaattgcc
      841 gcgttatgga ccgaatcctc ctcggagatg acgggcgtaa cctctgcgcg gatggtggcg
      901 ttgaagtggg cgatctccag ggcgtagtta ttcgggctct cgacctgaaa ggtccgactc
      961 ccctcttctt tcaccaccgt gtaggtgagg gtcccggttt cattgaaggg ccgttcggta
     1021 taaagggccc cccgagtccc ctgcgcttcg aagaacatcc gccggctttg ctcgaaagat
     1081 acgtggaatg acatagtcgg tcctcgatcg aatcgtagca aggcggtcgc ggacaggtct
     1141 accccttcaa ccgggtgaag ggaagcgacc gcgtgcgcca ctgtcggctc tttttcggtg
     1201 agataacgcg caaggtgaac aagataacag cccaagtcat aaactgcgcc cccgcccaat
     1261 tccctcgacc agcgaatatc cttcggtcgc ttcgacaggt cgaaggaaaa cgaggtcata
     1321 agatgacgta tctcgccgat ctcgccagtt tgaagggcct ttcgtaggtg ctcaatcaac
     1381 ggattatgga tataggcgaa gccctccatc aggattttac ccgtgccccg cgcgacgcga
     1441 aacatctctc tggcatccaa agagttcaag gccaagggct tttcacagag cacgtgtttt
     1501 ccggccagga gcgcgttcgt gacccattct ttatgcaagg cgttgggtag gggaacgtag
     1561 acggcatcga cttctcggtc gttcagtagc tgggtatacg tgtcatacgt tttcgcgggt
     1621 tgaaacaggg agaaaaatcg tcgcgctttc tcccggtctc tactggcaac ggcctccagc
     1681 acgcaaccgg gggtttgtaa aatagcagga atcacggcct tctcggcaat cctcgccgcg
     1741 ccaagcacac cccattttac ggttttcaac acatctcaca cctccattct tttctcttac
     1801 gattataacc taaaaaaacc ggtttccggg ttgggaatac cggtcaagtt atggggtgac
     1861 atgactaaga ggttcatcgc ggggtaacca gagggctgac ttcggaaccc cacgaagtct
     1921 cgcgatacgc acttgggaac accaaacttt agtttggcat gtcttctatg ttttcatctt
     1981 tctccgtgcc ctctgtgccc tctgtgcgaa ctcgattttt tgtttttaat ctaggctttt
     2041 tttctttgtg ttctctctgt gccc
//
LOCUS       DAOPXN010000272         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_59102_length_2047_cov_6.020582, whole genome shotgun sequence.
ACCESSION   DAOPXN010000272 DAOPXN010000000
VERSION     DAOPXN010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59102_length_2047_cov_6.020582"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            414..1754
                     /locus_tag="PKM49_10065"
     CDS             414..1754
                     /locus_tag="PKM49_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNY83180.1"
                     /translation="MRIAVIGSGTSAIGFLYHLLKTHPEYEVDIFERGLEPARRAAEG
                     ESILYGFGGAGTFSDGKLSLSPQIGGEVADYIGYESFKKYLPECLELWTLGFDRKALE
                     RSDLSEGPRVWDIKRAFNENRMDLTLGEFIHVGTDNLATVIQRLYSLLSSHKSFHFHF
                     STPIKDYSHADTAVIDETGRSYGPYDQVILAVGRSGSVLLNRILELSGADFTSNTVDL
                     GVRFEMPAILTDEITQILYEFKVSYITAQDDKVRTFCVNPRGYVVREDGSDFSLVNGH
                     SYTHQKSDLTNFAILVTHKFTEPFKDSFEYASSIARLANKLAGSGKILVQRYKDFKAL
                     RRSTNSKMQKSFVVPSLKGATPGDLNLILPRRTAADIEEFIEQLSRVIPGANNPDNLL
                     YGIEAKFYSTKPDFDQWFRIKGAPIRIIGDASGYSRGIVQATISGIALAETIGL"
BASE COUNT          504 a          530 c          515 g          498 t
ORIGIN      
        1 cgcgaaaaga aggggcgctt acgccgcgat gactacaagg ttcatcgcgg ggtaagtggt
       61 ttttacgacc ccgcgaaaaa atctgccgac ttaaacgttg gcgttcccaa cccccagtgt
      121 ctcgcgatcg gctctgctca cgagttatcg cggggtaacc aacggaaccc cgcgatccta
      181 tctttgcttt tttgcgccgc gatgagcccg tacaaatgcg tatcgcgccg tcgcgaaatg
      241 aaggggcgct tacgccgcga tgactacctg gacaaaaatc gtgcggtcga ttctagcctt
      301 gtttcttgat tacgccgtat aacttgacac acacgccttg tggtgttcga tctcgtttac
      361 ggtataatcg taatcagtac tagaaaccaa ccgatagaag gggcgaaata actgtgagaa
      421 tcgcagtgat aggatccggc acctcggcga taggattttt ataccatcta ctgaaaacgc
      481 atcctgaata cgaagtcgat attttcgaaa gggggttaga accggcccgt cgcgccgcag
      541 aaggagaaag catcttgtac gggttcgggg gcgccggcac cttttccgac gggaagttgt
      601 ccctttctcc tcaaattggc ggggaggtgg cggactacat agggtatgag tccttcaaaa
      661 aatacctgcc tgagtgttta gagttatgga cgctcggatt cgatcggaag gcgcttgaaa
      721 ggtccgacct aagcgagggg ccgcgggtct gggatatcaa gagggctttc aacgaaaatc
      781 gtatggacct cacgcttggc gaatttatcc acgtgggaac cgacaatctc gcgaccgtca
      841 tccaacgcct ttactcctta ctcagctctc ataaaagctt tcacttccat ttttccactc
      901 cgattaagga ctattcgcac gcggacaccg ccgttatcga cgaaaccggc cgctcttacg
      961 gcccctacga tcaggtgatc ttagcggtcg gccgttccgg gagcgtactt ttaaaccgaa
     1021 ttttggaact gtcgggcgcg gatttcacca gcaataccgt cgacttggga gtccgtttcg
     1081 agatgccggc gatactcacc gatgagatca cccaaatcct ctatgagttt aaggtcagct
     1141 acatcactgc ccaagatgac aaagtccgta cgttttgcgt gaatcctcgt gggtacgttg
     1201 ttcgggaaga cggatcggat ttctctttag tcaacggtca ttcctacacc caccagaagt
     1261 cggacttgac caacttcgcc atcctcgtca cccataaatt caccgaacct tttaaggatt
     1321 ctttcgaata cgcttcctct atcgctcgat tggcgaataa actcgcggga tccggaaaaa
     1381 tcctagtaca gcgttacaaa gacttcaaag cgttgaggcg atcgacgaac agcaagatgc
     1441 aaaaatcctt cgtcgtcccg tctttaaaag gggcgacccc gggggatttg aatctcatat
     1501 tacctcgtcg aacagcagcc gatatcgaag agttcatcga gcaactttca cgagttatac
     1561 caggcgcgaa caatcccgat aacctgctct acggcatcga ggcgaagttt tattcgacga
     1621 aaccggattt tgaccagtgg ttcaggataa agggagcgcc tatccgcatc atcggtgacg
     1681 ccagcgggta ctctcgcggt atcgtacagg caacgatcag tggtattgca ttagcggaga
     1741 ccattggttt gtgagtatca tatcgaggcg caacaataaa acggaaagaa gaggagattc
     1801 gtcttttgcc ctttttacgc cgcgatgctc ttgtggggtt atagcggggt aagcgcattt
     1861 tacggcccag aaaaaaactg tcaacttaaa agttgacgct cccaaggcgt tctcaacggc
     1921 gttcataggc gttctcagtt tgatcccaaa acgtatcaca tcggaagatg ctttttcagc
     1981 tcctcctcgt ttcgcatatc tatcaggtcc aagcccaagg tatcgagttg gtctatcgtg
     2041 agcgctt
//
LOCUS       DAOPXN010000273         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_60576_length_2015_cov_4.409694, whole genome shotgun sequence.
ACCESSION   DAOPXN010000273 DAOPXN010000000
VERSION     DAOPXN010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60576_length_2015_cov_4.409694"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(196..1656)
                     /gene="guaB"
                     /locus_tag="PKM49_10070"
     CDS             complement(196..1656)
                     /gene="guaB"
                     /locus_tag="PKM49_10070"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNY83181.1"
                     /translation="MEFLEGLTFDDVLLCPHYSEVVPTQTDLTSRFTRKITVRVPLVS
                     AAMDTVTESAMAKSLARVGGIGVIHKNMSIAKQAEQIDLVKRTENGIIFDPVSIHPGM
                     KVKEAERLMREYKIGGLPVIDHEKTLLGIVTNRDIRFEDDENRLVEDLMTPFDRLVVA
                     KSGISLEEAKKVLHRHKVEKLPIIDDQRKIVGLITIKDIKSVVEFPNASRDSKGRLMV
                     AGAVGTSDDTMERVRALIAAGVDAIVVDTAHGHSKNVVNRVAEIKRLYPQMQVIAGNI
                     ATYEAALSLHQAGADAVKVGIGPGSICTTRIVAGVGVPQLSAIFDCAKASRELDLPII
                     ADGGIRYSGDIAKALAAGASTVMIGSIFAGAEESPGETIIYDGRTFKAYRGMGSIGAM
                     QAGSSDRYNQFGINAEKLVPEGVEGMLPFKGRVSDIVYQLCGGLRSGMGYCGAATIPQ
                     LQQNARFVRITPAGMKESRPHDISITKESPNYRFQI"
BASE COUNT          435 a          576 c          521 g          483 t
ORIGIN      
        1 agtcagcccg ccgattaccc cgcgatgaaa ctcggaggca tcgcggcgta agcgcctctt
       61 cttttcgcga cggcgcgata cgtttttgag aaagacgaag ggcggatcgc ggggtcgtaa
      121 aaaccattta ctccgtaatg aaactcgcaa tcatcaagcg ccgagctttt gttcggcgta
      181 ctcagcagcg tcaggctata tttgaaaccg gtagttcggc gattccttcg tgatcgagat
      241 gtcgtgcggg cggctctctt tcatacctgc gggggtgatt ctgacgaatc tggcgttctg
      301 ttgcagttgt gggatcgttg cggcgccgca ataacccatg cccgaccgca gtccgccgca
      361 taactgataa acgatatcgc tcactctccc tttgaagggt agcatccctt cgacgccttc
      421 ggggacgagt ttctccgcgt tgattccgaa ctgattgtaa cgatcgctgc tgccggcttg
      481 catcgccccg atgctcccca tcccacggta ggccttaaat gttcggccat catagatgat
      541 cgtttcgccc ggcgactctt ccgctcctgc gaagatagac ccaatcatca ccgtagaggc
      601 cccagcagcg agggctttgg caatgtcgcc ggagtaccga ataccgccgt cggcaataat
      661 cgggagatcc aattcccggc tggctttagc gcaatcgaag atggcggaca gctgcgggac
      721 gccgactccc gcgacgatac gggtcgtgca gatcgatcct ggccctatcc cgactttcac
      781 cgcgtcggcg ccggcttgat gcaaactgag ggccgcttcg taggtcgcga tattgcccgc
      841 gatcacctgc atctggggat acaggcgctt gatctcggct acacgattga ccacgttttt
      901 ggagtggccg tgcgcggtgt ccacgacgat ggcatcgacc ccggcggcga tcaacgcgcg
      961 aacgcgttcc atcgtatcat cggaagttcc aacggctccg gccaccatca accgaccttt
     1021 cgagtcccgt gacgcgttag gaaattcgac aacgcttttt atatccttta ttgtgatcaa
     1081 tccgacgatc tttcgctggt catcgatgat cggcaatttt tcgaccttgt gtcggtggag
     1141 caccttcttc gcttcctcga gtgaaatgcc ggacttcgcc acgacaagcc tgtcgaaggg
     1201 cgtcatgagg tcttccacca agcggttctc gtcgtcttca aaccgaatat cccgattcgt
     1261 cacgataccc agtaaggtct tttcgtggtc gataacggga aggccgccga tcttgtactc
     1321 ccgcatcagt cgttcggcct ctttcacctt cataccgggg tgaatagaca ccgggtcgaa
     1381 gatgataccg ttctccgtac gcttgactag gtcgatctgc tccgcttgtt ttgcgatcga
     1441 catattttta tggatgaccc cgatccctcc gacgcgggcc aaggacttcg ccatcgcgct
     1501 ttcggtcacc gtatccatcg ccgcgctcac gagcgggacc cgaacggtta tcttgcgggt
     1561 gaaacgcgaa gtcaggtcgg tttgcgtggg taccacttct gagtaatggg gacatagcag
     1621 cacatcgtca aaggtcaggc cttctaagaa ttccatttta tgcgcctcct cgatttttcg
     1681 atattctacc acgtcttcga tgaaagcgaa ttttcgaacc tgtttcaagg aacgtctggg
     1741 aacgacaatt ttaaagttgg catagtttaa aaaagggctc gcacagagag cacggagcaa
     1801 taagttagga gcaaacatgc cacactaaag tgtggcgttc ccagccgttc ggcacgcaca
     1861 gacgtacggt gctcctagcc attcgaaaaa cttgccacac taaagtgtgg cgttcccaag
     1921 gagtcaaaac ccaggccgaa ctaaagttca acgttcccag gcgcattcgc cctatttgtt
     1981 tacaaacgat aattaaaaca gtctatattt taaac
//
LOCUS       DAOPXN010000274         2007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_60941_length_2007_cov_5.511270, whole genome shotgun sequence.
ACCESSION   DAOPXN010000274 DAOPXN010000000
VERSION     DAOPXN010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60941_length_2007_cov_5.511270"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..>2007
                     /locus_tag="PKM49_10075"
     CDS             83..>2007
                     /locus_tag="PKM49_10075"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNY83182.1"
                     /translation="MGVRQADLFDRPAVRVVDAETIEGYFLFNDLFGNETFILKTETP
                     RLIYNRGIQATRYLVLFALALVVLSLVTFVSVSYKYVLSQLLEIGRTVRKIISTKELS
                     SRIPPLRRDEVGTLGQDINAMLDTIEKDQNILRESEARLKRGERFGRMGYWEYDLAND
                     SVVWTEGLYALYELDPKHPDSPKDIFFRGFLPEEKTLLRAAMESIRTGGLYRYIHRII
                     LPDGATKYIKEEANGIYEGVKLVKVVGTTQDVTESVLNQKALEGSQDQLKMINFELEI
                     MNRELQEANTRADHFSKMLENLLHLTTHISDNAQGNVTDFLNQLLTISLFLVENIQSA
                     GIFVREDERYVLKFSRRPKVSEYVLPTVETALLEPLKMRPLHIEWDKVVKGSAFDQIK
                     LGLGEYVADVKESLFIPLTMKHEVYGALSLEASKQYPGKIQEEDKRIAESLGSIGSSF
                     LTLQNALASQDQFQKELIMSMIQILELYDPYTKGHSEKVAKYAYTIARKMKLSDDSAK
                     QLYWTGLVHDIGKLLIPRETLNKPTKLTKEEFEGVKNHSVWGSKVLSSSERTRDIAKI
                     VLYHHERIDGLGYPEGLKGDQIPLEARIIAVADAYDAMTNDRSYKKKIPIADALRELE
                     RCKGTQFDSAIVDIFIR"
BASE COUNT          534 a          480 c          553 g          440 t
ORIGIN      
        1 gactaaagaa atcgaaaaga tcacatcgct gtttccgacg tcttttagta ttgtcccttt
       61 ggctgaaacg actttggagg gggtgggcgt acgccaagcc gatctgtttg accgtccagc
      121 ggtgcgcgta gtggacgcgg aaacgatcga aggttatttt cttttcaacg acctattcgg
      181 taacgaaacc ttcatcctaa aaacagaaac accacgactg atctataatc gcggtataca
      241 ggccactcgc tatctcgttc ttttcgctct ggcgctcgtc gtgctctcgc tggtcacatt
      301 cgtttccgtg tcgtacaaat acgtgctgtc ccagctcctc gagatcggcc gaaccgttcg
      361 aaagatcata tcaacgaagg aactgagctc tcgtatcccc ccactgcgga gagacgaggt
      421 cggaacgctg ggccaagaca tcaatgcgat gctcgatacg atcgaaaaag atcaaaacat
      481 ccttcgcgaa agcgaggcgc gattgaaacg gggggagagg ttcggtcgta tggggtactg
      541 ggagtacgat ctggcgaatg acagcgtggt atggacggag gggttgtacg ccctctatga
      601 gttagatccg aaacatcccg actcgcccaa agatatcttt ttcagggggt ttttacccga
      661 ggagaaaacg ttgcttcgag cggcgatgga atccattcgt acgggcgggc tctatcggta
      721 tatccatcga ataatactcc ccgatggcgc cactaagtat atcaaggaag aggcgaacgg
      781 tatttacgaa ggggtgaagt tggtcaaagt ggttggaacg acacaggacg tcacggagtc
      841 cgttctgaac caaaaagctc tggagggctc tcaggatcag ctaaaaatga tcaatttcga
      901 gctggagatc atgaaccgtg aactccaaga ggccaacacc cgagcggatc atttttcgaa
      961 gatgttggag aacctgctcc atctcacgac gcatatttca gacaacgctc aaggcaatgt
     1021 caccgatttt ctcaaccagc tcctgacgat ttccttgttc ttggtggaaa acatacagtc
     1081 tgcggggata ttcgtgagag aggacgagcg atacgtccta aaattcagcc gtcggccgaa
     1141 ggtaagcgaa tacgtgctcc caacagtgga aaccgcgctt ctagagccct tgaaaatgcg
     1201 cccgcttcat atcgagtggg acaaagtcgt gaaagggtcg gctttcgatc aaatcaaact
     1261 gggtttaggg gagtatgtgg ctgacgtgaa agagtcgctt ttcatcccgt tgacgatgaa
     1321 gcacgaggtg tatggagccc tgtctttgga agcttccaag cagtatccgg ggaagataca
     1381 agaagaggac aagagaatcg cggagtctct cggaagcatc ggctcttcgt tcttgacgct
     1441 tcaaaacgcg ctggcatcgc aagatcagtt tcagaaggag ctgattatgt cgatgatcca
     1501 gatcctcgag ttgtacgatc cctataccaa agggcactcg gaaaaggttg ccaaatacgc
     1561 ctacacgatc gcccggaaga tgaagctgag cgatgacagt gccaaacagc tctattggac
     1621 cggcttggtg cacgatatcg gcaaactctt gattccgaga gagactctga acaaaccgac
     1681 aaagctgacg aaagaggagt tcgagggcgt gaaaaaccat tccgtatggg ggtcgaaggt
     1741 cctcagcagc tcggagcgca cccgtgacat cgccaaaatc gtcttgtatc accacgagcg
     1801 gatagacgga ttgggttatc cggagggtct caaaggggat cagattcctt tggaagcgag
     1861 aattatcgcg gtggcggacg cctacgacgc gatgacgaac gatcgttcct acaagaaaaa
     1921 gataccgata gccgacgcgt tgcgggagct ggaacggtgt aaaggtaccc aattcgactc
     1981 cgcgatcgtg gacatattca tacggga
//
LOCUS       DAOPXN010000275         1972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_62592_length_1972_cov_3.873761, whole genome shotgun sequence.
ACCESSION   DAOPXN010000275 DAOPXN010000000
VERSION     DAOPXN010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62592_length_1972_cov_3.873761"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            395..>1972
                     /gene="infB"
                     /locus_tag="PKM49_10080"
     CDS             395..>1972
                     /gene="infB"
                     /locus_tag="PKM49_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNY83183.1"
                     /translation="MRIYELAKQLGMSSKELIEELRELGLEAKNHMVVIEEEQAKILA
                     DLFGNKAQTPEKPVEQMVAVEEPPAPPPEKPTVAVTEAPKDSEQKDVKKAQKEKSTKS
                     RTAGRPQKEPSGGRAPTRKDSENVLFVNVLSMTVRELAETMKIPVNKIIQDSFSKGII
                     MNPNQRLEYDSFAELAKKYGFTVKISDEEDLVEKKEDDSERQLREYYETLYRDKKHEL
                     QSRPVVVTVMGHVDHGKTTLLDRIRKARVAEGEAGGITQEIGAYQVEYKNQKITFIDT
                     PGHEAFTEMRARGAQATDLVVLVIAADDGVMPQTEEAYNHAKEAKVPVIVAINKVDKP
                     NANIELTKQQMVSKLHLVPEDWGGDTVTVNISAKKGEGIEDLLEMILLVSEMANIRCH
                     AKGKARGVIIESRLDPKVGPTATCILKDGVLRVGNFFVAGNTWGKVKALINDKGKQVK
                     EGKPSEAVRIIGIDQVPDAHSILYAVESPNVAKGIVERIHLKEKADQLKRKHISLEDF
                     YQMVKEGDRKVLKMVLKA"
BASE COUNT          559 a          468 c          552 g          393 t
ORIGIN      
        1 cgcggggtaa ccagcgggct gacttcggaa gctcacgaag tctcgcgata cgctccgctg
       61 cctttctctt ttaaaacgtg ccgaaatgaa gtttggcgtt cccaggcttt cccaaggtgt
      121 tcacaagatt cttttctctg tgttctctgt gccctccgtt cgagcccttt tattcaaggc
      181 acgtacaaaa gcgtatcgcg ccctcgcgaa aagaaggggc gcttacgcca cgatgactat
      241 atggacttaa aatcgcgcga tcgattctcc ccttgtttct tgattacacc ccataacttg
      301 acacacacgt atcggttgag agcgctgaat ccgtggtata atcgaacgat agttttcggg
      361 tatttgcgtt tggaggtgac ccttattggc gaaaatgagg atatacgagt tggcgaaaca
      421 gctgggaatg tccagcaaag agttgatcga ggaattgcga gaattagggt tagaagccaa
      481 gaaccatatg gtcgtgatag aggaagagca ggcaaagata ttggccgatc tgtttggaaa
      541 caaagcgcag actccagaaa aaccagtcga acagatggtg gcggttgaag aaccccctgc
      601 tccaccgccg gagaaaccga ccgttgcggt aaccgaagcg cctaaggact ctgaacagaa
      661 agacgtaaaa aaggctcaaa aagaaaaatc gacgaagtcc cgcacggcgg gtcgtccgca
      721 aaaagagcct tccggtgggc gcgcgcccac gaggaaagac agcgaaaacg tcttgttcgt
      781 caacgttttg tcgatgaccg tccgtgagct cgcagaaacg atgaaaatcc ccgtgaacaa
      841 gatcatccaa gactcttttt ctaaagggat cattatgaac cccaaccaac ggttggaata
      901 cgatagtttt gccgaattgg cgaaaaagta cgggttcacc gttaaaatat cggatgagga
      961 agacttggtc gaaaagaagg aagatgacag cgagcgacag ctgcgtgagt actacgaaac
     1021 gctgtacaga gacaagaagc acgaacttca gagtcgcccg gtcgtagtca cggtgatggg
     1081 ccacgtggat cacggaaaaa cgacgttgtt ggatcggatc aggaaagcca gggtcgcgga
     1141 aggagaagcc ggcggcatca cgcaagagat cggggcttat caggtcgaat ataagaatca
     1201 aaagatcacc tttatcgata cccccggaca cgaagctttt acggagatga gggccagagg
     1261 cgcgcaggcc acggacttgg ttgttttggt catcgccgcg gatgacgggg tgatgccgca
     1321 aaccgaagaa gcgtataatc acgccaaaga ggcgaaggta ccggtcatcg tcgcgatcaa
     1381 taaagtggac aaaccgaacg ccaatattga actcaccaaa cagcagatgg tctccaaact
     1441 acacctggta cccgaagact ggggaggaga taccgtgacg gtcaacatct ccgcgaaaaa
     1501 aggcgagggt atcgaagacc ttctcgagat gatccttctc gtttctgaga tggccaacat
     1561 ccgctgccac gcgaagggaa aggcgcgcgg cgttatcatc gaatcacggc tcgacccgaa
     1621 ggtaggcccg acggccactt gtattctcaa agacggtgtt ttgagagtgg gcaacttttt
     1681 cgtcgcaggg aatacctggg gtaaggtcaa agcgttgatc aacgacaaag ggaagcaagt
     1741 gaaggaagga aagccttcgg aggccgttcg tatcatcgga atcgaccagg ttccggacgc
     1801 gcattcgatc ctgtacgcgg ttgaatcacc caacgtcgcg aagggtatcg tcgagagaat
     1861 ccacctcaag gagaaggccg atcaactcaa gcgaaaacac atctctttgg aggattttta
     1921 ccagatggtg aaggaaggcg accgcaaggt tttgaagatg gtcctcaagg cg
//
LOCUS       DAOPXN010000276         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_62914_length_1965_cov_5.792147, whole genome shotgun sequence.
ACCESSION   DAOPXN010000276 DAOPXN010000000
VERSION     DAOPXN010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62914_length_1965_cov_5.792147"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="PKM49_10085"
     CDS             <1..1004
                     /locus_tag="PKM49_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNY83184.1"
                     /translation="EGDTPLLEADASLKAETGIENLWIKDETRNPTGSFKDRGSLFCL
                     WMADEMGESALATVSTGNMGHSIAAYAARSGKRALIFVPEFAPMEKVLPMAFFGAEVF
                     RVRSPHYSEMKSKVLETASARGVRVVTGNGPIRCEGYKCLAFELYEQLKGQAPDYIAL
                     PISACGHIRGIFKGFRELMAAGFLTRLPAMVAVQASRNAPIVQAIQSESGEIHPFPVY
                     PTVAEAITSTNPPGGSEIVEKAKRYRWPAETATEEEIIKAHQRLAMSGFFVEPSSATV
                     LPALKKLRRRGVIPPESCVVMVLTGSGMKDLSLLPRKAADTRVVGLEDLEKVLSERS"
     gene            1014..>1965
                     /locus_tag="PKM49_10090"
     CDS             1014..>1965
                     /locus_tag="PKM49_10090"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HNY83185.1"
                     /translation="MSKPDRIDGHFDLLMSLDHRRRIGQKNVIETQFLPSFRAGGFTG
                     MIASVFIEDRFLPEMGLRKALDQIGSLYEDRRESQGTWDICKTYEDYERCRQAGRFGF
                     FLSLEGADPLMNDLGLLRVFYELGIRFLGLVWSRRNYAADGCHFKPVSEGRKGGLTDF
                     GVALLKKGEELGMIIDVSHLNDEGFEDVYAYASKPFIASHSNCRALMDTARNLRDIDI
                     RRIAEIGGLIGGNAANIFIKDPPREATVRDFAKHLAHMKRLTGIDHVALGFDFCIHLR
                     EDSPRPSSTDTGGIPVFREIIDGHPELTRLEEALREEGFNP"
BASE COUNT          449 a          501 c          601 g          414 t
ORIGIN      
        1 gagaaggcga caccccgcta ctggaagcgg acgcgagttt gaaggcagag accgggatcg
       61 agaatctctg gatcaaggat gaaacccgta accccactgg cagtttcaag gaccgggggt
      121 ctttgttttg cctatggatg gcggacgaga tgggagaaag cgccctggcg acggtttcaa
      181 cgggcaacat gggccattcg atcgcggcct acgcggcccg ttcgggaaag agagcgctca
      241 tcttcgttcc ggagttcgcc ccgatggaaa aggtcttgcc gatggcgttc ttcggcgcgg
      301 aggtctttcg cgtccgatcc ccgcattact ccgagatgaa gagcaaagtg ctcgaaaccg
      361 cttccgcacg cggggtacga gtggtgacgg gaaacgggcc gatccgttgt gaagggtaca
      421 aatgcttggc ttttgaactc tacgaacaat tgaagggaca ggcaccggac tacatcgccc
      481 ttccgatatc cgcctgcggg catatcaggg gtatcttcaa aggctttcgc gagctgatgg
      541 ccgccggttt tctcacacga ctaccggcta tggtggcggt ccaggcctcg cggaacgcgc
      601 ccatcgtcca agcgatccaa agtgaatcgg gcgagatcca tccctttccg gtttacccca
      661 cggtggccga agcgatcacg agcacgaacc cgccgggcgg ttccgagatc gtggaaaagg
      721 cgaaacggta tcgctggccg gccgaaacgg cgacggagga agagatcatc aaagcccacc
      781 aaaggctagc gatgagcggc tttttcgtgg aaccctcctc cgcgacggta cttcccgcgt
      841 taaaaaaact tcgtcgaaga ggtgtgattc cccctgagtc gtgtgtggtc atggttctga
      901 ccggctctgg gatgaaagac ctctcgttgt tgccacggaa agcagcggac acccgtgtcg
      961 tgggtctgga agacctggag aaggtcttgt ccgaacgatc ataggaggag aaaatgtcca
     1021 agccagatcg aatagacggt cacttcgatt tactgatgtc gcttgatcat agaaggcgga
     1081 tcggtcaaaa aaacgttata gaaacgcagt ttctcccttc ttttcgggcg ggagggttca
     1141 ccggaatgat cgcgtcggtt ttcatagagg accgattctt acccgagatg ggattacgaa
     1201 aggccttaga ccagattggg agcttgtatg aagaccgccg ggagagccaa gggacgtggg
     1261 atatctgtaa gacctatgag gattacgagc gatgtcgaca agccgggcgt ttcggtttct
     1321 tcctttcgtt ggaaggagcc gaccccttga tgaacgactt gggtctcctc agggtgttct
     1381 acgaattggg aatccgattt ttgggcctcg tctggagccg tcggaattac gctgcggatg
     1441 gctgccattt caaaccggtt tcggaaggcc gaaagggcgg tctgacggat tttggggtag
     1501 cgttgttgaa aaaaggggaa gagttgggga tgatcatcga cgtgagccac ctgaacgacg
     1561 aagggtttga ggacgtctac gcctacgcga gtaaaccgtt catcgcctca cactccaatt
     1621 gccgggcgtt gatggacacc gccaggaacc ttcgggacat cgatattcga cggattgccg
     1681 aaatcggcgg gctgatcggc ggcaacgcag cgaacatttt tatcaaagac cctcctcgtg
     1741 aagccaccgt gcgggacttc gcaaaacatc tggcccatat gaaacgccta accggcatcg
     1801 atcacgtggc tttagggttt gatttctgta tacaccttag ggaagacagt ccgaggcctt
     1861 cgtctacgga taccggcggt atacccgtct ttagagagat catcgacggc catccggaac
     1921 tgacccgttt ggaagaggcg ttgcgagaag aggggttcaa cccgg
//
LOCUS       DAOPXN010000277         1935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_64392_length_1935_cov_4.354255, whole genome shotgun sequence.
ACCESSION   DAOPXN010000277 DAOPXN010000000
VERSION     DAOPXN010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64392_length_1935_cov_4.354255"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            614..1606
                     /locus_tag="PKM49_10095"
     CDS             614..1606
                     /locus_tag="PKM49_10095"
                     /inference="COORDINATES: protein motif:HMM:NF022779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3137 domain-containing protein"
                     /protein_id="HNY83186.1"
                     /translation="MKTVEEFERYFYRHLLAELSELEKERNKIRIGIIVIFAISVLLL
                     VTLLCLFGRSLQMMASLCFFVLFLVGIGFFLLWNLLSAGISKGFVQRFKKTVIDGIVR
                     FIEPSLTYNPKSYITWLDFQRSDLFRDRVDRYTGEDLVYGKYGETEIRLSEIKAEKKV
                     RSSNKNATERWQTVFSGTFLIADFHKDFFGKTVIFPDFAERLFGPLGQAIQGMRLNRG
                     ELIKLENPEFEKYFVVYGSDQIESRYILSPSFMERLVTYRKKVGSQVYFSFVNSYIFI
                     AIPTKRNLFEPNLLKNLTDFNHIKTYFEDLSLLIGAVEDLNLNTRIWSKQPYER"
     gene            1685..>1935
                     /locus_tag="PKM49_10100"
     CDS             1685..>1935
                     /locus_tag="PKM49_10100"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNY83187.1"
                     /translation="MRKKNRVHNASASLDAILKKRYDLIPNLTEVVKGYAAYEQAVLE
                     SVLRVRNEALRARTIEEKEHADGKIAKGIEEIFVIAEKYP"
BASE COUNT          473 a          461 c          526 g          475 t
ORIGIN      
        1 cgtagtcatc gcggcgtaag cgccccttct tttcgcgacg gcgcgatacg cttttttcag
       61 gggatatcgc gacaaatagg aaaagggcgt atcgcggggt tccgttggtt accccgcgat
      121 aacccgtgag cagagccgat cgcgagacac tgggggctgg gaacgccaac gtttaagttg
      181 gcaggttttt tcacggggtc gtaaaaacca cttaccccgc gatgaacctc gtagtcatcg
      241 cggcgtaagc gccccttctt ttcgcgacgg cgcgatacgc ttttttcagg ggatatcgcg
      301 acaaatagga aaagggcgta tcgcggggtc ccgttggtta cccccgcgat tagcctctta
      361 gctatgtcac cccgtaactt gacccgtatg cgatattgaa ctcataggaa taatcgggta
      421 taatcaaaag gctgagtctg tcgtgcgccc gagcgaatcg caggtgttgt ggcgtagcct
      481 tgcgagtacc ggtaaactcg ggaatcggaa aacgatgctt ggggcataag cgagagaaac
      541 aacaagccgc ggacggagac tggcgcggca gctgtcactc cggcaaggga cctaccgata
      601 gggaggttgg gttttgaaaa ccgtcgagga gttcgagcgg tatttttaca gacatttgtt
      661 ggcggaatta tccgagttgg aaaaagaacg gaataagatc cgtatcggga taatcgtcat
      721 cttcgcgata agcgtgctac tcctcgtcac tctgctttgc ctgttcgggc gatcacttca
      781 aatgatggct tcgctctgct ttttcgtcct ctttttggtc ggtatcggct ttttcttgct
      841 ttggaacctg ttgagcgcag gcatttcgaa gggttttgtc cagcgtttca agaagacggt
      901 gatcgatggg atcgtacgtt tcatagaacc ctcgttaacc tacaacccga agtcgtacat
      961 cacttggttg gactttcaac gaagtgacct gtttcgtgac cgggttgacc ggtacacggg
     1021 cgaagacctc gtctacggga agtacgggga aacggagata cggttgtcgg agatcaaggc
     1081 tgaaaaaaag gtcaggtctt cgaataaaaa cgcgacggaa cggtggcaaa cggtttttag
     1141 tggcaccttc ctcatcgcgg actttcacaa agattttttc ggcaagaccg tgatctttcc
     1201 ggactttgcc gaaaggcttt tcgggcctct cggtcaagca attcaaggaa tgcggctcaa
     1261 ccggggggag ttgatcaaac tcgaaaaccc cgagttcgaa aaatacttcg tggtctacgg
     1321 gagtgaccag atcgagtcgc gctatatcct atccccctcg tttatggaga gactggttac
     1381 ttatcgtaag aaagttggtt ctcaggttta cttttctttc gtcaactcct atatcttcat
     1441 cgcgatcccc acgaaaagaa accttttcga gccgaatctg ctaaaaaacc taacggattt
     1501 caaccacatc aaaacctact tcgaagacct ttccctgctg attggcgcgg tcgaagacct
     1561 taacctgaac acccggattt ggagcaagca accgtatgag aggtgagtta tggggacttc
     1621 ccttattttt cttctaattc tcgttgggat cgccctcttg tttattgggg tatggaatag
     1681 ttttatgagg aaaaagaatc gtgtccacaa cgcgtccgcc tcattggacg ccatccttaa
     1741 aaagcggtac gatctcatcc ccaacttgac cgaagtcgtg aaggggtacg ccgcctatga
     1801 gcaggccgta ctcgagtcgg tcctcagagt gcgaaacgaa gcgttaagag cgcggacgat
     1861 agaagaaaag gagcacgcgg atgggaaaat agcgaagggg atagaggaaa tcttcgtgat
     1921 tgcggaaaaa taccc
//
LOCUS       DAOPXN010000278         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_65462_length_1914_cov_5.513717, whole genome shotgun sequence.
ACCESSION   DAOPXN010000278 DAOPXN010000000
VERSION     DAOPXN010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65462_length_1914_cov_5.513717"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKM49_10105"
     CDS             <1..443
                     /locus_tag="PKM49_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012004064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HNY83188.1"
                     /translation="AIWLGNKFLAMPALIFMQFIMGGGSTIVLLSAAMASIPDYYFEA
                     ARLEGASSWKVFTKVTIPLLKPTILYALIMGTIANFQTFSNVYIMTRGGPEFSTISIG
                     YLVYTTAFREYNLGLASAMSMVMFGILALLGFLQFKWLGSNVEY"
     gene            458..1291
                     /locus_tag="PKM49_10110"
     CDS             458..1291
                     /locus_tag="PKM49_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HNY83189.1"
                     /translation="MTPWKAKQKVKMVLSLAVLTLFGLLAFIPLYWMVITAIKPASLT
                     LRFPPELFPKNPTLANFQELFQRPLILRWTFNSGVVAVSVTFLQLLLCTMAGYSLAKK
                     RFPLSQLLFFVYIASMMIPKQVTLVPLYILVSKFGMINTYWGLILPSVAAPFGVFLMR
                     QFLVTVPTEIIEAAKIDGASEWRTFYKIIVPISLPAMAVLGIFTFVAEWNDFLWPLIV
                     TNTQQMKTLQAGLALLQEEVPMQYAFLMSGATYAAVPMIIIFFAFSKYFLKGVTVGAI
                     K"
     gene            1442..>1914
                     /locus_tag="PKM49_10115"
     CDS             1442..>1914
                     /locus_tag="PKM49_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNY83190.1"
                     /translation="MEKKRFYVTTPIYYVNSEPHIGSAYTTIVADMIARYQRLRGYDV
                     YFLTGTDEHGQKILETSQKAGVDVQLFCDKWASKFKELWKDLDISNNDFIRTTQPRHT
                     YVVQAFVKRLFDRGHVYKGVYEGWYCIHEEAFWSEEDLIRDNGRRVCPTCKREVR"
BASE COUNT          441 a          488 c          488 g          497 t
ORIGIN      
        1 ccgccatttg gctggggaac aagtttctcg cgatgcccgc gttgatcttc atgcaattca
       61 ttatgggcgg cggttccacg atcgtcctcc tttctgccgc gatggcatcg attccggact
      121 attacttcga agccgccaga ctcgaagggg cctcatcgtg gaaagtcttt accaaagtga
      181 cgatacccct actcaagcca acgatcctat acgcgctcat tatgggaacg atcgcgaact
      241 tccaaacctt ttcgaacgta tacataatga ccagaggggg acccgagttc tccacgatta
      301 gcatcggata cttggtatac accacggcct tcagggaata caacctcggc ttggcttccg
      361 cgatgtcgat ggtgatgttc ggcattttgg ccctcctggg tttcttgcag ttcaaatggc
      421 ttggctccaa cgtcgagtat tagggggagg gttgagtatg acgccttgga aagcaaaaca
      481 gaaagtaaaa atggtcttgt ctttggccgt cttgacgctt tttggcctgc tggccttcat
      541 tccgttgtat tggatggtca tcaccgcgat caaacccgcc tccttgacct tgcgctttcc
      601 tcccgagctc tttccgaaaa acccgacgtt agcgaacttc caagagctct ttcaaagacc
      661 gttgatactg agatggacct ttaacagcgg ggtagtggcg gtgagcgtta cattcctcca
      721 gctgttgttg tgtacgatgg cggggtattc tctggcaaaa aagcgcttcc ccttgagcca
      781 gcttcttttt ttcgtctata tcgcttccat gatgatccct aaacaggtaa cgctcgttcc
      841 cctgtacatc ttggtctcga aattcggtat gatcaatacc tattgggggt tgatccttcc
      901 gagcgtcgcc gcgccttttg gggttttctt gatgcggcag ttcctggtga ccgttccgac
      961 agagatcatc gaagcggcga aaattgacgg tgcgagcgaa tggcggacct tctacaaaat
     1021 tatcgttcca atctctctac cggcgatggc cgtgttgggg atttttacct tcgtcgccga
     1081 atggaatgat tttttgtggc ctctgatcgt gacgaacacc caacagatga agacgctcca
     1141 agccggtttg gcgcttctgc aggaagaagt gccgatgcag tacgcgttcc tgatgtccgg
     1201 ggcaacctac gctgcggtcc cgatgattat catattcttt gccttctcca aatacttctt
     1261 gaaaggcgtt acggtcggag cgatcaaatg agtactttag aagctcgccc gccttccggg
     1321 agagatcgtc gcgccatcgg tgagcacgcg tcatagggcg taaaactccc tcgtctgtgg
     1381 tataattctg cggaaaatga aggatttccg atttttttgt tgcaaaggcg gaggaatcag
     1441 gatggaaaag aaaaggtttt acgtcaccac gcccatctat tatgtgaact cagaacccca
     1501 tatcggttcc gcttacacga ccatcgtcgc ggatatgatc gcgcgatacc aacgcttgcg
     1561 cggatacgac gtctacttct taaccggaac cgacgaacat gggcagaaga ttctggaaac
     1621 ctctcaaaaa gccggggttg atgttcagct cttctgcgat aaatgggcga gcaaattcaa
     1681 ggaactttgg aaagacttgg atatctcgaa taacgacttt atcagaacga cccaaccgcg
     1741 acatacctat gtcgttcaag ctttcgtgaa gcgcttattc gacagagggc acgtttataa
     1801 aggcgtctac gagggatggt actgcattca cgaagaggcc ttttggtcgg aagaggacct
     1861 tattcgagac aacggccgac gagtctgccc aacctgcaaa cgagaagtgc gctg
//
LOCUS       DAOPXN010000279         1869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_67910_length_1869_cov_5.677508, whole genome shotgun sequence.
ACCESSION   DAOPXN010000279 DAOPXN010000000
VERSION     DAOPXN010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67910_length_1869_cov_5.677508"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1166
                     /locus_tag="PKM49_10120"
     CDS             438..1166
                     /locus_tag="PKM49_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNY83191.1"
                     /translation="MDEIIQTIREELERLADPTVREREQRFFKEPVELYGVKANLVTK
                     IANATWKRVKAYDKPEIFAICEELYSSGKLEEASIASKWGQGFDAFTEKRDMERYLSW
                     IHQYVTNWATCDGLCNHAVGDILMKYPELVAHMYELAKSENRWARRAGAVSLILPARK
                     GYFKKEAFEICDILLHDPEDLVQKGYGWLLKEVSRLHGREVLDYVQKRKRTMPRTALR
                     YAIELMPEADRKAAMAKEPVEKSR"
     gene            complement(1265..1450)
                     /locus_tag="PKM49_10125"
     CDS             complement(1265..1450)
                     /locus_tag="PKM49_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83192.1"
                     /translation="MENGTLREFVLSKQGLGLSKDDIYRLALLSFGTQWENLILMILC
                     EIEREEKHQGTRASDPQ"
BASE COUNT          491 a          471 c          524 g          383 t
ORIGIN      
        1 tgccaactta aaagttggcg ttcccagaaa ccatgccaaa ctaaagtttg gcgttgccgg
       61 gcgttcccaa gaacctttac tctgtgttct ctgtgccctc cgtgtgaacc ctgcttcatt
      121 tttaagacat gccaacttaa aagttggcgt tccaaagagt atcgcgccgt cgcgaaaaga
      181 aaaagcgctt acgccgcgat gctgcgctcg ggaacactcc cagtgaaaag atagaactat
      241 gccaaacaaa agtttggcgt tcccagggtg ttcccaactt gacacacacc acataaaaga
      301 tgtagtttat gataggcttc ttctcagatt tcttcagttg agttcgttcc tcgagtggaa
      361 gggtatcagt cgaggagagg ctggcgtgcg tagagatgaa aaaacgaagc gagaagaacg
      421 agggagggga aagacagatg gacgagataa tccaaaccat ccgagaagaa ttggaacggt
      481 tggcggatcc gacggtaaga gagcgcgagc agcggttttt caaagaacct gtcgaactct
      541 atggagtcaa ggcgaacctc gtgacgaaaa tcgcgaacgc gacgtggaag cgggtgaaag
      601 cgtacgataa gcccgaaatt ttcgcgatat gcgaagagct gtacagttcc ggaaaattgg
      661 aagaggcttc cattgccagc aaatggggac agggcttcga tgcgttcacg gagaagcgag
      721 atatggagcg ctatctttcg tggatacacc aatacgtgac gaactgggcg acgtgcgacg
      781 ggctctgcaa ccacgccgta ggtgacatcc tcatgaaata ccccgaattg gtggcccata
      841 tgtatgaact tgccaagagc gaaaaccgat gggcgcggcg ggccggcgcc gtttcgctga
      901 tactcccagc gcgaaaaggg tacttcaaga aggaagcttt tgagatctgc gacatcctat
      961 tacacgatcc ggaagatttg gtacaaaaag ggtacggttg gctcttgaag gaagtgagcc
     1021 ggctacacgg gcgtgaggtc ttagactacg tacagaagcg caagcggacg atgccccgca
     1081 cggccctgcg ttatgcgatc gaactaatgc cggaagcgga ccggaaagcc gcgatggcaa
     1141 aggaaccggt cgagaaaagc cggtagggta gtattcggac accccgtgcg ggtgatcgat
     1201 tgcgacgagc gaggccgaaa atcccctcct ggcgacgggc ggaatcccat agaggtatca
     1261 ttcattattg aggatccgaa gcgcgcgtac cttgatgttt ttcctcgcgc tcgatttcgc
     1321 ataaaatcat caggatcaag ttctcccatt gagtaccgaa agacaacaaa gccagtcgat
     1381 agatatcgtc cttagacaaa cccagtcctt gtttcgacag aacgaactcg cgcagggtcc
     1441 cattttccac cgacactgcc tcctcttcgg gcgcaaaagc gataacctta cgtacttaag
     1501 tgtaccacat caacccgata gcgcccttcg aaagccgtcg gagcgccgga cttctgggca
     1561 cgcctttctg ggagcgcctt gggaacgctg agctttgtgg gtcatcgcag ggtaagtgat
     1621 ttttacgacc ccgcgatacg ctctttcttc ttctcaaaat cgtatcgcgc cgacgcgaga
     1681 agaagggtcg cttacgccgc gatgccaccc acgcgggcgc gcctgggaac acttggaaac
     1741 gccgcacctt agtgcggcat gttttctgag cacgcccttt tgggagctcc ctgggagcga
     1801 cgcactttag tacatccaga taaggcggaa atgttcagca ggaagaaagc aagcctgccg
     1861 gggaaaaag
//
LOCUS       DAOPXN010000280         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_68380_length_1861_cov_7.065891, whole genome shotgun sequence.
ACCESSION   DAOPXN010000280 DAOPXN010000000
VERSION     DAOPXN010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68380_length_1861_cov_7.065891"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1682
                     /gene="miaB"
                     /locus_tag="PKM49_10130"
     CDS             363..1682
                     /gene="miaB"
                     /locus_tag="PKM49_10130"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNY83193.1"
                     /translation="MRFYIETYGCQMNVNESEKMAGLLSNAGHVYTERMKDADLILVN
                     TCTVRQKSAEKVYGFIGRTLALRRINPSLRLGVCGCLAEAEGRALLRRKGVDFVFGTR
                     AYPAIPEMLERVRRSERFVERGDFLAQENACSPIRRFSSHHAWVTIIYGCDKFCSYCI
                     VPYTRGREKSREFSDVCAEVESLAKLGYREITFLGQNVDSYGKDLRENSVGFADLLSF
                     AAKVKGIERIWFLTSYPSDFTQGAIDAIANHPNIARAIHLPLQSGSDSILKKMNRRYS
                     RAEFIDLTERIRKAIPEAALSTDLIVGFPGESEEDFERSVDLVETVGFERLNLAMYSP
                     REGTLSQQTLPDDIPYSVKNKRLQFLLNLQKRINKALNTASLGKTQWVIAEKPTKPGS
                     PQLYGRTDRNRIVIFEGDADLIGKWVEVSIKKVTAGPLYGDISNIRD"
     gene            1706..>1861
                     /locus_tag="PKM49_10135"
     CDS             1706..>1861
                     /locus_tag="PKM49_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83194.1"
                     /translation="MQSNVKITPMVQQYLEIKGQFLDALLLFRLGDFYEAFFDDAKLV
                     SKTLQLVL"
BASE COUNT          443 a          518 c          511 g          389 t
ORIGIN      
        1 tcgtcccctt ctttcgcgtt tcttttattc tatcacaatc gaggttttcg gtgggggtat
       61 tgggaaagcc tgggaacgcc aactttcaag ttggcatgcc ttaaaaacag ggttcgcaca
      121 gagggcacag agaacacaga ggaaaaatag acaagggaca gtgttctcag ggaaaaagaa
      181 aagacgtgcc gaaccaaagt ccggcgttcc caagcgttcc caagcggtca aaggtaggga
      241 tcgcggggtc ccttcggtta ccccgcgatt accccttaag cactcccagt tcgcacgccc
      301 atacggattt tccgtgttat aattgaaaag caacggattc ggatagatcg gaggcgtttg
      361 atttgcgttt ttatatagag acctatggct gccagatgaa cgtgaacgag tcggagaaga
      421 tggcagggtt gttgtctaat gccgggcacg tctacaccga acgtatgaag gatgcggacc
      481 tcatcctcgt gaatacgtgc acggtgcgcc agaagtccgc cgaaaaggtc tatgggttca
      541 tcggtaggac cctcgcgttg cgccgaatca acccgtcttt gcggttgggc gtttgcggtt
      601 gcctggccga agcagaaggc cgcgccctct tgcgccgcaa aggtgtcgat ttcgttttcg
      661 gcacacgcgc ctatcccgct ataccggaga tgttggagcg ggtgcggcgg tcggaacgat
      721 tcgtcgagcg aggagacttt ctcgcccagg aaaacgcctg cagccctatc cggaggttct
      781 cttctcacca cgcgtgggtc accatcatct acggatgcga caagttctgc tcctattgta
      841 tcgtccccta cacacggggt agagagaaga gccgcgagtt ttcggacgtc tgcgccgaag
      901 tggagtcgct ggccaaactg gggtatcgcg agatcacctt tttagggcag aacgtcgatt
      961 cctacgggaa ggacttgcgc gaaaactccg tcggattcgc cgacctcctc tccttcgccg
     1021 cgaaggtgaa agggatagag cgtatctggt tcttgacatc gtacccgagc gacttcacac
     1081 aaggagcgat cgacgcgatc gcgaatcatc cgaatatcgc ccgggccatc cacctacctc
     1141 tccagtccgg cagcgactcg atcctgaaaa aaatgaaccg ccggtattcc cgggcggagt
     1201 tcatcgatct gaccgagcgc atacgaaagg ccattcccga ggctgcccta agcaccgacc
     1261 tcatcgtcgg attccccggg gagtcggaag aggacttcga aagaagcgtc gacttggttg
     1321 aaacggtcgg cttcgaacgc ctgaacctcg cgatgtattc tccccgagag gggacgctat
     1381 cccaacaaac gctccccgac gatatcccct attcggttaa aaataaacgg ctgcagttcc
     1441 tcctgaacct gcagaagcgg atcaacaaag ccctcaacac cgcgagttta ggaaaaacac
     1501 agtgggtcat cgccgaaaaa cccacgaagc ccggttcgcc acagctttac gggagaacag
     1561 accggaaccg aatcgtgatc ttcgaaggag acgccgacct gatcggcaaa tgggtcgagg
     1621 tctccatcaa aaaggtgacc gccggcccgc tctatggcga tatttcaaac attcgcgact
     1681 gaaccggcgc gcaaagagga gagctatgca atcgaacgtc aagatcaccc ctatggtgca
     1741 gcagtacctg gagatcaaag ggcagtttct agacgccctc cttctcttcc gcctcgggga
     1801 cttttacgag gccttcttcg atgacgcgaa gttggtttcc aaaacgctac agctcgtttt
     1861 g
//
LOCUS       DAOPXN010000281         1841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_69550_length_1841_cov_6.180291, whole genome shotgun sequence.
ACCESSION   DAOPXN010000281 DAOPXN010000000
VERSION     DAOPXN010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1841
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69550_length_1841_cov_6.180291"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..>1841)
                     /gene="nagZ"
                     /locus_tag="PKM49_10140"
     CDS             complement(379..>1841)
                     /gene="nagZ"
                     /locus_tag="PKM49_10140"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNY83195.1"
                     /translation="RKGVSGVIFFARNLVEPLQVWNLTRLVRELFPFEPFIMSDEEGG
                     DIKRLTSGVTRGINAFGLAATGNPENARRMTQAIGEDLRAVGINTALSPVLDVNSESE
                     NPIIGSRAFSDRPETVIEYARPYAQGFKEFGILTVGKHFPGHGPAKVDSHLDLPVVDL
                     SRGQLEEHLRPFATLVREGLLDMILSAHILYPQISDEPATLSRVFLTEILRKDLEFDG
                     LAITDCMEMNAMKVRFGVERATVRAFNAGMDLILISHTPELQRKAMAAFIDAVESGEI
                     SYRRVEESLERIERVRSRLYAQTPRSERSLPFSQVVDYFGRKTEELDMKLAEESIAIH
                     HGEMRPYLKDEPIFVFDFMPTITGIGEENTTAIAMKTAFGERFAQFDYYTLPYAESAK
                     EVLDRIEGKRGRMILITRTRGKAPAQKVQDLLNTLCERFPDNLHVSGRDPYDISVVER
                     SKTAIATFGLSPYQLKGLMRVLSGDSTPKGRMPTML"
BASE COUNT          381 a          502 c          518 g          440 t
ORIGIN      
        1 gcccgaatat tacacgcgcc aacggggatg accggcagag gtggagtagg gaggcgccca
       61 gcgaccgaaa gcgctcaccg gttaaaggtc acataaccct tttgacgaag ccgtttggaa
      121 agccgtatgc gggaaatccg cacgtacggt ttgaggtggc aggagaagga aaccgggaga
      181 gggtaagctg ccaggtagtg tgaaggacac tcacacacag aaatcgagag cggtacatct
      241 ctgtcttcat ggcgccttct cttgacccta cttggttacc ccgcgatgaa cctcttagtt
      301 atgtcaccct aaaaattgtc ctacacggca gcgccgttct ttctatcagt ttcgcgtacg
      361 cgtccctctg aaagcccttt acagcatggt cggcattcgg cctttgggtg tcgaatcgcc
      421 tgataaaacc cgcatcaggc ctttcaattg gtaaggggaa aggccaaatg tcgctatagc
      481 cgttttcgag cgctcgacca cgctgatatc gtaagggtcc cgtccgctca cgtgaaggtt
      541 gtctgggaac cgttcacaga gcgtgtttag caagtcttgc accttctggg caggcgcctt
      601 gcccctggtt cgggtgatga gaatcatacg gccacgtttt ccctcgatac ggtccaaaac
      661 ctcttttgcg gactctgcgt aaggaagggt atagtaatca aattgcgcga atcgttcgcc
      721 gaatgcggtt ttcatagcga ttgccgtcgt attctcttcg ccaattccgg tgatcgtggg
      781 cataaagtcg aacacgaaga tcggttcgtc ttttagatag ggacgcatct ctccatggtg
      841 gatcgctata ctctcttcag cgagtttcat atccagctct tcggtttttc taccaaagta
      901 gtccacaacc tggctaaatg ggagggatcg ttccgaacgt ggggtttgag cgtataaccg
      961 gctgcgtacc ctttcgatgc gttccaggct ttcttcgact cgccgatacg agatttctcc
     1021 actttctacc gcgtcgatga aggcggccat cgcttttctc tgcagttccg gcgtgtggct
     1081 gatcaagatc aaatccatcc ctgcgttgaa agcgcggacg gtggctcgct ccacaccgaa
     1141 acgcactttc atcgcattca tttccatgca gtcggtgatc gctagtccgt cgaactcgag
     1201 gtcctttcgg aggatttcgg tgagaaagac gcgagagagt gtggcgggtt cgtcgcttat
     1261 ttgagggtac aggatgtggg cgctcaaaat catatccaac agcccttctc ggaccaacgt
     1321 ggcgaagggg cggagatgct cttcgagctg ccctctggaa aggtcgacga cgggcaggtc
     1381 caagtgggag tccactttcg ccggaccatg gccaggaaag tgtttcccga cggtcaggat
     1441 gccgaattcc ttgaaccctt gcgcgtaggg cctcgcgtat tcgatcaccg tttccggccg
     1501 atccgagaaa gcgcgcgaac cgatgatcgg gttttccgac tcgctgttga cgtccagcac
     1561 ggggctcagg gcggtgttga tacccaccgc gcgcaggtct tctccgatgg cttgggtcat
     1621 ccgtcgggca ttttccgggt tgcccgtggc ggcgagccca aaggcgttga ttcccctcgt
     1681 caccccggac gtgagccgtt tgatgtctcc gccctcttcg tcagacatga tgaagggttc
     1741 gaaaggaaac agctctcgaa ccagtcgggt gaggttccat acttgtagcg gttcgaccag
     1801 gtttcgagcg aaaaaaatga cccccgagac tcccttccgc g
//
LOCUS       DAOPXN010000282         1837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_69796_length_1837_cov_5.144781, whole genome shotgun sequence.
ACCESSION   DAOPXN010000282 DAOPXN010000000
VERSION     DAOPXN010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69796_length_1837_cov_5.144781"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PKM49_10145"
     CDS             <1..571
                     /locus_tag="PKM49_10145"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNY83196.1"
                     /translation="VDHPRVVLIAGSVPYGIPLDLYPKLTKIAHEKDTEVYLEARGIL
                     LTHSVSTYCADVVRPDLRSDTRVFGKEVETMADYIEAGEKIVEKGAKLVVSSYQVVND
                     LVFTKDGVWLLSLADPRVDPSHLLGAGDTYMAAMIYHSRKYGTDLLERAKFGMAAALA
                     KTKYLFKKSPTIQEVEESLPKIQTKQIKG"
     gene            577..1059
                     /locus_tag="PKM49_10150"
     CDS             577..1059
                     /locus_tag="PKM49_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNY83197.1"
                     /translation="MLVKDAMVRDVSAIFEDEKIETFIRISALHLQHVFPVVDEKMKI
                     IGIISEDDVISETIPSYFGLLHSASFIPDTNQMKRELSEIKDEPIGKFIKRTVITIEE
                     NDTILHAADLLLRHKLLNLFVVDKDGRLTGIINRSRILLAVLHEVLEEKTAQEKPNRL
                     "
     gene            1056..>1837
                     /locus_tag="PKM49_10155"
     CDS             1056..>1837
                     /locus_tag="PKM49_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNY83198.1"
                     /translation="MIPSISFLTFGCKVNQYESDWMARQLANGYSSSTGEEKRPDLVI
                     VNTCTVTAEADRKNLQAIHRSKRRYPDAKVLVMGCHTQLNPEVYVGLADGIVGNGEKL
                     DIVHYAERVLAEPVFSEVNRSYWLRSPLRMCLEGRFGLSRAFALIQDGCTTGCTYCKI
                     YHARGPRSRSKPLEEALSEIRAFSENAYREIVLLGINVGQYQDGSNTLVDLLERALPA
                     FPNLRFRLSSINPEHLRPELLALWRYPNLCKHLHLSIQSGSAS"
BASE COUNT          469 a          478 c          469 g          421 t
ORIGIN      
        1 ggtcgaccac ccgagggtcg tattgatcgc cggttcggtc ccgtacggga ttccactcga
       61 tttgtacccg aagctcacaa aaatcgccca cgaaaaggac acagaggtgt acttggaggc
      121 gcggggcatt ctactcaccc attcggtcag tacgtactgc gccgacgtcg tacgccccga
      181 tctacgaagc gatacgcgag ttttcggaaa agaggtggaa acgatggccg attacatcga
      241 ggcaggcgaa aagatcgtcg aaaaaggggc caagttggtc gtttcttctt accaagttgt
      301 caacgacctt gtgtttacga aagacggagt ttggcttctt tcgctagccg atcctcgtgt
      361 ggacccttcg catttgttag gcgcgggaga cacctatatg gcggcgatga tttatcactc
      421 acgaaagtac ggcacggatc tgttggaaag ggcgaaattc ggaatggccg ccgcgttagc
      481 aaaaacgaaa tacctgttca aaaagagccc gaccatccaa gaggtcgaag agtcactacc
      541 aaaaatacaa actaaacaaa taaagggttg agagagatgc tcgtaaaaga tgcgatggta
      601 cgcgacgttt cagcgatttt tgaagacgaa aagatcgaga cctttatcag gatttcggct
      661 ttacaccttc aacatgtctt ccccgttgtc gatgaaaaga tgaaaatcat agggatcatc
      721 agcgaagacg atgtgatcag tgaaaccata ccctcctatt tcggcctttt acattccgct
      781 tctttcattc cggacaccaa ccaaatgaag cgcgagttat cggagatcaa agatgagcct
      841 atcgggaagt tcatcaaacg caccgtgatc acgatagaag agaatgacac gatcctccac
      901 gccgccgatc ttttgttgcg gcacaagctt ttaaacctgt tcgtggtgga caaagatggc
      961 cgcctaactg gcatcatcaa tcgttctagg attctcctcg ccgttcttca cgaggttttg
     1021 gaggaaaaaa ccgcgcagga gaaaccgaac cggctttgat tccttcgatc tcctttctaa
     1081 ccttcggctg caaggtgaat caatacgaat ccgattggat ggcccgccaa ttggcaaacg
     1141 gatactcgag ctcgaccgga gaagaaaaac gtccggacct cgtgatcgtg aacacctgta
     1201 cggtcactgc cgaagcggac cgcaagaacc ttcaagctat ccatcggagc aaacggcgtt
     1261 atccggacgc gaaagtgctc gttatggggt gtcataccca gttgaaccct gaggtgtacg
     1321 tagggctggc ggacgggata gtaggcaatg gagaaaagct cgacatcgtg cattatgcgg
     1381 agagagtcct cgctgaacct gttttttccg aggtaaatcg gtcctattgg ctgcgatccc
     1441 ctctgcgcat gtgcttagag ggacgttttg gtctatctcg cgcctttgcc ctcattcagg
     1501 atggatgtac caccgggtgc acctactgca agatttatca cgcgcgaggg cctcggagcc
     1561 gttcgaaacc ccttgaagaa gcgctttccg agattcgcgc cttttcggaa aacgcctacc
     1621 gcgagatcgt gttgttaggc atcaacgtcg gccagtatca agacgggtcc aacacactcg
     1681 tcgatttgtt ggaaagggcg ctcccagctt tcccgaatct acggtttcgt ctgagctcta
     1741 tcaaccccga acacctacgc ccagaactgc tcgccctttg gaggtacccg aacttgtgca
     1801 aacacctcca cctttccata caaagtggtt ctgcgtc
//
LOCUS       DAOPXN010000283         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_71367_length_1811_cov_6.496583, whole genome shotgun sequence.
ACCESSION   DAOPXN010000283 DAOPXN010000000
VERSION     DAOPXN010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71367_length_1811_cov_6.496583"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="prfA"
                     /locus_tag="PKM49_10160"
     CDS             <1..843
                     /gene="prfA"
                     /locus_tag="PKM49_10160"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNY83199.1"
                     /translation="EEIPSLEKRLKEDERALLVGILPPDPAAKNSAIVEIRAGTGGEE
                     AALFAGSLFRMYNRYSDIRHWKTEILDSNPTDLGGFKEIVFKIKGKGAYGALKYEGGV
                     HRVQRVPETEASGRIHTSSASVVVLPEITADMEIDISPEDIKIDVYRASGAGGQHVNR
                     TESAVRITHLPTGIVVTCQNERSQHQNKLQALDVLKAKLYEIRMSEEQQKLSQKRKHF
                     IGTGNRNEKIRTYNYPQSRLTDHRVNYTSYQLQAFLDGDIDELLAILQEEDTIERLKE
                     VFGE"
     gene            847..1368
                     /locus_tag="PKM49_10165"
     CDS             847..1368
                     /locus_tag="PKM49_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83200.1"
                     /translation="MLVEVLSGLWLAAILVCILLCQQGYILERAQSKNLELQRVSNGR
                     QMLCLFEGDFFARRVSPIYRCQNKAGTGYEIAFWERTQGVEKFVIYYFQTRKNYEVYY
                     AKRCVIDLGNILTTEDQAYMRGLIAKIEAGTFAIPDQRVGGTNLTSLCATAMLKKEGP
                     LLYYQDFETCFFF"
     gene            1389..>1811
                     /locus_tag="PKM49_10170"
     CDS             1389..>1811
                     /locus_tag="PKM49_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83201.1"
                     /translation="MKESALFLGVLIYLITLSFAAPTIAPKPQSVVIPNTLCLQTAKN
                     SLYAGYSDGKIACWNLATGELETLIPAPTNGSPVLSLAISPNGLLLAAGHQDGTLRLW
                     NLQPAQLYGEVIRENNAIWACLFTEDSRYVYTGNADGRL"
BASE COUNT          475 a          462 c          481 g          393 t
ORIGIN      
        1 gaggagattc cttctttgga aaagcggttg aaagaagacg aacgcgcgtt gttggtcgga
       61 atcctaccgc cggatccggc ggctaagaac agcgcgatcg tcgaaatccg tgccggaacc
      121 ggcggggaag aagccgcttt gttcgcggga agcctatttc ggatgtacaa ccgttactcc
      181 gatattcgcc attggaagac cgagatactc gatagtaatc ccacggatct aggcggattc
      241 aaagaaatcg ttttcaagat aaagggcaag ggagcctacg gcgcgctcaa gtacgagggg
      301 ggagtccatc gcgtgcaacg cgtgccagaa accgaagcga gcggaaggat ccatacctct
      361 tccgcttctg tggtagtctt gcccgaaatc accgcggata tggagatcga tatttctcca
      421 gaggatatca agatcgatgt ctatcgcgct tcgggggcgg ggggccaaca tgtcaatcgc
      481 acggagtccg cggtgcggat cacccacctt ccgaccggca tcgtcgttac ctgtcagaat
      541 gagcggtcac aacaccaaaa caagctgcag gccttggatg tcttaaaggc gaaactctat
      601 gagatacgaa tgtcggaaga acagcagaaa ctctctcaaa agcggaaaca tttcatcgga
      661 acggggaacc gaaacgagaa gatacggacc tacaattatc ctcaaagccg attgacagac
      721 catcgggtca attacacgtc ctatcagttg caagcctttt tggacggaga tatcgatgaa
      781 ctgctggcga tccttcaaga agaagacacg atcgagcgtc ttaaagaagt gttcggagag
      841 tgaccgatgc ttgtggaagt attgagtggc ctgtggcttg cggcgatact ggtttgtatc
      901 ctcttatgcc agcaaggcta tatcttggaa agagcgcaga gtaaaaacct ggaactgcag
      961 cgtgtttcca acggaagaca gatgctgtgt ctctttgagg gagatttttt cgcccgccgc
     1021 gtctctccga tctaccgttg ccaaaacaaa gcgggcacgg gctacgagat cgccttttgg
     1081 gaacgaacgc aaggcgtgga gaagttcgtg atctattatt ttcaaactcg gaagaactat
     1141 gaagtctatt acgccaaacg atgtgtcata gacctcggta acatactcac cactgaagac
     1201 caagcgtata tgagaggttt aatcgcgaag atcgaagccg gaactttcgc aataccggat
     1261 caacgcgtcg ggggaacaaa cctgacgtct ctgtgcgcca ccgcgatgct gaaaaaggaa
     1321 gggccgctcc tctattatca agatttcgaa acttgtttct tcttttagcg ccgaaaggag
     1381 aaagtcgctt gaaagaatcc gctctttttt taggggtttt gatctacctc ataacgcttt
     1441 ctttcgccgc accgacgatc gcgccgaaac cacaaagcgt cgtcataccc aacacattat
     1501 gccttcaaac ggcgaaaaac agcctgtacg ccggttattc cgatggaaag atcgcctgct
     1561 ggaacctcgc gaccggagag ttggaaaccc tcataccggc gccgaccaac ggctcgcccg
     1621 tgctttcgct cgccatctcc ccgaacggcc tattactggc cgcgggtcat caagacggta
     1681 cgttgcgcct ttggaatctc caaccggcac agctctacgg tgaagtcatc cgagaaaaca
     1741 acgcgatctg ggcttgcctt tttaccgaag acagccggta tgtttatacc ggaaacgcgg
     1801 acggaaggct t
//
LOCUS       DAOPXN010000284         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_72847_length_1788_cov_5.011541, whole genome shotgun sequence.
ACCESSION   DAOPXN010000284 DAOPXN010000000
VERSION     DAOPXN010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72847_length_1788_cov_5.011541"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="PKM49_10175"
     CDS             complement(<1..1184)
                     /locus_tag="PKM49_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83202.1"
                     /translation="MESSGIKKAIILCFSILVINLLFFIVAQLCLVSPGSGGSALPFF
                     TGLWPASFVVAFKREALHFYREHSPFWFAGFSLLNLSFHTFLLRSPSKKESDAPLANA
                     VEIVGLLAFGLVALFLASSQRAFVPGILFSPQNESLLFRWVVYFFVFFALGQNLDSAD
                     KTFFNRLLVIDGIGTLLLLFRIPMVGILLLAGNLCLFLVRSFRSSSIWLTIGYAAVLS
                     GLVFDGWHVYRSIPSPIGFFILGVLVYLFFLFLELLQASKRETAALKARIDALQAEKT
                     IQRLLLQEKEEEARKDSEALKTFEQLILTTNALYEKALLVKEDYLHELLEHARLLIPE
                     ADGGMLVLAENGGWRSHFSLGAVPQSLKRDPIDPSVFHVLKTHTATRFYDANVFLTTA
                     HVV"
     gene            complement(1211..>1788)
                     /locus_tag="PKM49_10180"
     CDS             complement(1211..>1788)
                     /locus_tag="PKM49_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNY83203.1"
                     /translation="TSKASQMIEDFLILVKSQQSKKKLQLSNSKRFSDFVDARFGFVG
                     ERKSQIPVFASAFDLPLACEVFLPIHDRTVLKGATAFLPFFEPIRIGDRLVISTDSFQ
                     GVASRYEPFADHHFHVCLDAGPDEEHAPFESAMQILLAADLYRTLELKRRVLRQFNWV
                     IKLNAPVEDSRQDVSVLYEEGYQGVRRFLRS"
BASE COUNT          482 a          459 c          503 g          344 t
ORIGIN      
        1 acaacgtggg ccgtcgtcag aaacacattc gcgtcataga atcgggtggc cgtgtgggtt
       61 ttcagcacgt ggaaaacaga ggggtcgatc ggatcccttt tcagggactg tggaaccgcg
      121 ccgagcgaga aatgcgagcg ccaaccgcca ttttccgcaa gaaccaacat tccgccatcg
      181 gcttccggga tcaagagccg ggcgtgttcg agaagctcgt gaaggtagtc ttctttaacg
      241 agtaacgctt tttcgtaaag cgcgttggtg gtgaggatga gttgttcgaa ggtcttgagg
      301 gcctccgaat cttttcttgc ctcctcctct ttttcctgaa ggagaagcct ttgtatcgtc
      361 ttttccgctt gaagggcgtc gatccgggct ttcaacgccg ccgtctctcg tttcgaagct
      421 tgtaaaagct ctaaaaacaa gaagaagaga tagacgagga ctcccagaat gaaaaaacct
      481 atcggactcg ggatactccg gtaaacgtgc caaccgtcga aaacgaggcc ggatagaacg
      541 gccgcgtagc cgatcgtcag ccatatcgac gaggagcgga aggaacgaac gagaaagagg
      601 cacagattac cggccaagag aaggataccg accatcggaa ttctgaaaag gagcaacagt
      661 gtgccaatac cgtcgataac gaggaggcga ttgaagaagg ttttatcggc ggagtcgaga
      721 ttctgtccga gcgcgaagaa aacgaaaaag taaacgaccc agcggaacag gaggctttcg
      781 ttctgtgggg agaaaagaat tccgggaacg aaggcccttt gagaggaggc caagaacagg
      841 gccaccaagc cgaaggccag aagaccaacg atttccaccg cgttggcgag gggcgcgtcc
      901 gactcctttt tactgggcga gcgaaggaga aaggtatgaa acgagaggtt cagcagggaa
      961 aaaccggcga accagaaagg ggaatgctcg cggtaaaaat gaagagcctc tctcttgaag
     1021 gcgacgacga aactcgcggg ccacagccct gtgaagaacg gcaacgccga tccgcccgaa
     1081 ccgggtgaga ccaggcacag ctgagctact ataaagaaca gcaggttaat caccaaaatc
     1141 gaaaaacaca gtatgatcgc ctttttgatt ccggacgact ccatccgaca cctcttccgg
     1201 caaacaggaa tcaggagcgc aaaaaacggc ggactccctg gtacccttcc tcatatagaa
     1261 ctgacacatc ctgccgagaa tcctcgacgg gtgcgtttag cttgatgacc caattgaact
     1321 gtctcaaaac ccttcttttc aattcgaggg tccgatacaa gtccgcggcc aagagtatct
     1381 gcatcgcaga ctcaaacggc gcgtgctctt cgtcgggacc ggcatccaaa cacacgtgga
     1441 aatggtggtc cgcgaagggc tcgtagcgtg aggctacccc ttggaaacta tcggtgctga
     1501 tcaccagtcg atctccgatc cggatgggtt caaaaaaggg gagaaaggcc gtcgccccct
     1561 ttaggaccgt gcggtcgtgt atgggcagaa agacctcgca ggcgagcggg aggtcaaagg
     1621 ctgaagcgaa gaccggtatc tgactctttc tctcccccac gaacccgaaa cgcgcgtcca
     1681 cgaagtccga aaacctctta gaattcgaga gttgaagctt ctttttcgat tgctgggact
     1741 tcaccagaat caggaagtcc tcgatcattt gcgacgcttt cgacgtct
//
LOCUS       DAOPXN010000285         1779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_73424_length_1779_cov_5.204756, whole genome shotgun sequence.
ACCESSION   DAOPXN010000285 DAOPXN010000000
VERSION     DAOPXN010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73424_length_1779_cov_5.204756"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PKM49_10185"
     CDS             complement(<1..1091)
                     /locus_tag="PKM49_10185"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011187651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HNY83204.1"
                     /translation="MKEIAHNYLKLALKDPNATFREGQWDSIECLLNRKRVLVVQRTG
                     WGKSMVYFLAAKLSRDRGAGPTLLISPLLSLMRNQLEAAERIGVNARSINSSNRGDWD
                     RIKRELINNEVDLLMIAPERLANPEFRRSVLTHISHNIGLFVIDEAHCISDWGHDFRP
                     DYRRIVRVLEAIPSNVPILATTATANNRVVNDVIAQLGERLLVQRGGLGRKSLKLQNI
                     KIPQMSARMAWLAKTIPLLPGSGIVYTLTQRDTERVASWLKINNIDAEAYHAGISGED
                     GDSGHKETLEQKLLSNEIKVLVATVALGMGFDKPDLGFVIHFQRPASVVHYYQQVGRA
                     GRAVNEAYGVLLCGEEDETITNYFIDTAFP"
BASE COUNT          441 a          463 c          398 g          477 t
ORIGIN      
        1 ggaaaagcag tatcaataaa ataattcgtg atcgtttcat cctcttctcc gcacagaagg
       61 acgccatagg cttcgttcac cgctctaccg gcgcggccta cctgttggta gtaatgaacg
      121 acggatgccg gccgttggaa gtggatgacg aaacccaaat ccggtttgtc gaaacccatt
      181 ccaagagcca cagtcgccac caatactttt atctcgttac tcagaagctt ttgctccagt
      241 gtctctttgt gaccgctatc gccatcctcc ccggagatac ccgcgtgata ggcttccgcg
      301 tcgatattat ttatctttag ccaactggcg acccgttccg tgtctcgttg cgtaagcgtg
      361 tagacgattc cgcttccagg caatagcgga atcgtcttcg ccagccacgc cattctcgcg
      421 gacatttggg gtatctttat gttttggagc ttaaggcttt tacggcctaa ccctcccctt
      481 tgtacaagaa gcctttcgcc taattgcgcg atgacgtcgt tgacgaccct gttattcgct
      541 gtcgctgttg ttgcgaggat cggaacattc gaagggatcg cttccaatac acggacgatc
      601 cgacgatagt ccggtcgaaa atcgtgtccc cagtcggaga tacaatgcgc ttcatcgatt
      661 acaaaaagcc cgatattgtg agagatgtgc gtcaggacgc tgcgacgaaa ttctggattt
      721 gccaaccgct cgggcgcgat cataagcaaa tccacctcat tattgattag ttcgcgcttt
      781 attcgatccc aatcacctct attactgcta ttgatggaac gcgcattaac tcctatcctt
      841 tctgcggctt caagttgatt tcgcatcagc gacagtaacg gagaaatcag taatgtcgga
      901 ccggcgcctc gatcacgcga tagtttcgcc gctaaaaaat agaccatact tttaccccaa
      961 ccggttcgtt gcacaactaa aacccgtttc cgattgagta agcattcgat actatcccac
     1021 tgcccttcac gaaacgtcgc gttaggatcc ttaagcgcta acttaaggta gttatgggct
     1081 atttccttga ttcccaattt cgtcccccca ttctattctt attcgaaaca cgacaactac
     1141 agtgttaaaa gcctgattcc gaaggcttat aaaaaacgga aatatcttta ggatctccga
     1201 gacttgggtt ttccaggtga ctctgtggca ataggaacga tgacaaatga tgagacatca
     1261 tttggttact gacgaaaaat ggaaatacat tcacgttcgc tctcatttta ttgtaccacg
     1321 aattcagctg cttgtttatc ctaagttcgg ggatatatcg aggtggaata agaaaaaaga
     1381 agaaaaagaa gaacgaaaaa acggtccccg gaagtagaca ccataaaagc ttttttaacc
     1441 cgtaaagggc gtaaagaagc caattggtaa ggtcaagaga aggcgccgtg aagtccgaga
     1501 tgtaccgctc tcgatttctg cgtgtgaatg tccttcacac acctggcagc ttacccactc
     1561 cctgtttcct tttcctgcca cctcaaaccg tacgtgcgga tttcccgcat acggctttgc
     1621 aaacgtcttc gtcaaaaggg ttatgtgacc tttaaccgat gagcgctttc ggtcggtggg
     1681 gtgcctccct gctccacctc tgccgatcat ccccgttgtt gcgcgtaata ttcgggcgag
     1741 gagggccttt ccagttgcct gaaatgtcct tgtaaccgt
//
LOCUS       DAOPXN010000286         1717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_77577_length_1717_cov_5.107100, whole genome shotgun sequence.
ACCESSION   DAOPXN010000286 DAOPXN010000000
VERSION     DAOPXN010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77577_length_1717_cov_5.107100"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1717)
                     /locus_tag="PKM49_10190"
     CDS             complement(<1..>1717)
                     /locus_tag="PKM49_10190"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNY83205.1"
                     /translation="ALEHSSHRKELSIVNTENGPTTVRTKQELTLHTPILYRYRLSNG
                     KTKIVKTTPGRAIFNELLPEEYKQYDKVFKKKEIKNLVYEIFKKFGIDRTADLLDDIK
                     DLGFHYATVSGLTISVKDVLISAQRKKIIEKSEKRISEVEEYYRYGFLTPLERYREII
                     KIWDESTSDVLKATVKNMADHPYNPLQMMVSSGARGNEDQLKQLAGMRGLMAGPAGNI
                     IEIPIRANFREGLSVLEFFISTHGGRKGAADTALRTSNAGYLTRRLVDVAQSVVVSES
                     DCGTHKGIEATALRDEEVEIEGLEKNIFGRVIAEPIYEPELGTPLPHPLVPGKVYERD
                     TMLTGDDAKYLAKYEKRVPVKKEYEFNLSEDIRKDRLPYSYIELKEHVMAPTGMLLFQ
                     AETPVNFHLLQECKNNGIQRIAVVEYPIVGNFSKQKALSKDGKRTIVVEDEKIDPATA
                     RLLVEEEVTELRVRPTIIIRNVFTCESEDGVCAKCYGMDLSNHQIVNIGEAVGTVAAQ
                     SIGEPGTQLTMRTFHTGGIASTADITQGLPRAEELFEARKKLKENPAVFSTVRGFVKD
                     IKQEEN"
BASE COUNT          312 a          474 c          432 g          499 t
ORIGIN      
        1 cgttttcttc ttgtttgata tcttttacga atccacgaac cgtactgaat acggccgggt
       61 tttctttgag ctttttacgc gcctcgaaca gctcttcggc ccgtggtaac ccttgtgtga
      121 tgtcggcggt tgatgcgata ccgcccgtat ggaatgtacg catcgtcagc tgcgtccccg
      181 gttcgccgat cgactgggcc gcgactgttc ctaccgcttc tccaatattg acgatttggt
      241 gattcgacaa atccattcca tagcatttcg cgcacacgcc atcctctgat tcacaggtga
      301 acacgtttcg gatgatgatc gtcggtcgga cccggagctc ggtgacctcc tcttcgacca
      361 gcaacctcgc ggtcgccggg tctatcttct cgtcttccac gacgatcgtg cgttttccgt
      421 ctttggagag cgccttctgt ttggaaaagt ttcccacgat cggatactcg acgacggcta
      481 tcctctggat accgttgttt ttgcattctt gaaggaggtg gaaattgacc ggcgtttcgg
      541 cctgaaacaa cagcatcccc gtcggcgcca taacgtgttc tttgagttcg atataggagt
      601 aaggcagtcg atctttccgg atatcttccg atagattgaa ctcgtactct tttttcaccg
      661 gcacgcgctt ttcgtacttg gccaggtatt tggcgtcatc gccggtcagc atcgtatccc
      721 gttcgtagac ctttcccggt actaaggggt gtggtaacgg ggttccgagc tcgggttcgt
      781 agatcggctc cgcgatgacc cggccgaaga tgtttttctc gagtccctcg atctctacct
      841 cttcgtctcg gagtgccgtc gcttctatac ccttgtgggt gccgcaatcg gattccgaga
      901 cgaccacgct ttgcgcaacg tccaccagcc tacgggtcag gtagcccgcg ttagaggtgc
      961 gtaaggccgt gtctgccgcc ccttttcttc cgccgtgggt ggagatgaaa aactccagaa
     1021 cgctcaatcc ttcacggaaa ttcgcgcgga tcgggatctc gatgatattc cccgccggtc
     1081 cggccatcag cccgcgcata ccggccaatt gtttcaactg gtcttcgttt cctctggcac
     1141 cggacgatac catcatctgc aagggattgt atgggtgatc cgccatattc tttacggtcg
     1201 ccttcaacac gtcgctggtc gactcgtccc atattttgat gatctctcgg taacgttcca
     1261 agggcgtcaa gaaaccgtat cgataatact cttccacttc gctgatccgt ttttccgact
     1321 tttctatgat ctttttccgc tgggcggaga ttaaaacgtc tttgaccgag atggtcaaac
     1381 cggatacggt ggcatagtgg aagccgaggt ccttgatgtc gtccaacaaa tccgcggtcc
     1441 gatcgattcc gaatttttta aagatctcgt agacgaggtt tttgatctcc ttttttttga
     1501 acactttatc gtattgtttg tactcttcgg gtagaagctc gttgaagatt gcccggccag
     1561 gagtcgtttt cacgatcttc gtcttcccgt tcgacagccg gtatcggtat agaattggcg
     1621 tgtgcagcgt caattcctgt ttggtacgca ccgtcgtcgg cccgttttcg gtgttgacga
     1681 tcgaaagctc ttttctatgc gagctgtgct ccaatgc
//
LOCUS       DAOPXN010000287         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_83570_length_1637_cov_5.075221, whole genome shotgun sequence.
ACCESSION   DAOPXN010000287 DAOPXN010000000
VERSION     DAOPXN010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83570_length_1637_cov_5.075221"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PKM49_10195"
     CDS             <1..303
                     /locus_tag="PKM49_10195"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNY83206.1"
                     /translation="ITERQVEELTTIVRELEARVPLKGFVIPGSTLSSAKLDICRTVA
                     RRAERRIITLAAKEEVPIPVRQYVNRLSDVLFMLARLEEQHVGKILYKNDVKLQTE"
     gene            522..1619
                     /locus_tag="PKM49_10200"
     CDS             522..1619
                     /locus_tag="PKM49_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNY83207.1"
                     /translation="MCRKQRRAARQSCRYRIWLVVILTVFIPSILLPGGSPGLELSNE
                     EISWLRENSDKLLLYYTEDFPPAGYRSETGDFAGLGADVLSLIEKELGVSFKKTPFQN
                     WNAQLDALRTGRSAIAPTIVETEERRQYIFFTDPFAVVPVVIITSKNTKGTVSFQSLA
                     GKKVCAVSGSAPEKLLQGESLLYGIEIVQVESVQQGLESVAFGEASAFVESLAVASYM
                     IERLGYSNLRVGGETNYRFEWKLGVSKEYPLLFSAIQKALRHIPESELETIRNRWISF
                     GRNGGLDPTLRLWIGFSIGFLIILAISLSVLSFLLKRRLTQKVADLRKSEEKYRQTAD
                     NSPAVVYQLRLDPEGYASFLLNMCVVITKTK"
BASE COUNT          420 a          398 c          434 g          385 t
ORIGIN      
        1 ataaccgaac gtcaggtgga ggaactcacg acgatcgttc gagaactgga ggcgcgtgtg
       61 ccgctgaaag gattcgtcat tcccggtagc accctttcat cggctaagct ggatatctgc
      121 cgcactgtcg ctcggagagc cgaacggcgt atcattacct tagcggctaa ggaggaagtc
      181 ccgatcccgg ttaggcagta cgtcaatcgc ctttccgatg tcttgtttat gttggcccgc
      241 cttgaagagc agcacgttgg aaagatcctg tacaagaacg acgtaaaact tcaaacggaa
      301 taaaacgaga gtctagacca aatgaaacgc gcgcctatgg agcgcccagt tgcccttggg
      361 cgctctttaa tgtttgaagc cttcggtttc tccattaaaa acgttaatga actaaagggc
      421 tgaaatttcc tcgttttgtt gtaaaataga agagcaagaa gaaagtggaa gaggtcttac
      481 tttttgtaag tgccataaag accgtgaaag cagggggact tatgtgtcga aaacaacgaa
      541 gggcggcgcg tcaaagctgt cgatatcgga tttggctcgt tgtcattctc accgtattca
      601 tcccgtctat cctattgccg ggaggttcac ccgggctaga gctatcaaat gaagagatct
      661 cttggcttcg agaaaattcg gataaactgc ttttatacta caccgaagac ttccctccgg
      721 ctgggtatcg gtcggaaacg ggtgacttcg caggtttggg cgccgatgtc ctttccttaa
      781 tcgaaaagga actcggggtt tctttcaaga aaacgccatt tcagaattgg aacgctcaac
      841 tcgacgccct acgaacagga cgtagcgcaa tcgcgcccac gattgtcgaa accgaggaaa
      901 ggcggcagta catctttttc accgatcctt tcgcggtcgt cccggtcgtg atcatcacct
      961 ccaagaacac taagggaacg gtctccttcc aaagcttggc gggtaagaag gtttgcgcgg
     1021 tctcaggatc ggcgcccgaa aagctcctac agggcgagag cttgttgtat ggaatcgaga
     1081 tcgtgcaggt agaaagcgtc caacaagggc tggaaagtgt tgcttttggc gaggccagcg
     1141 ccttcgtgga gagtctggcg gtagcctcgt atatgatcga acgtttaggc tactccaacc
     1201 tcagggtcgg gggcgaaacg aactaccgct tcgaatggaa acttggcgtc agcaaagaat
     1261 acccactttt gttcagcgcg attcagaagg cgctgcgtca catccccgaa tcagaactcg
     1321 agaccatccg aaatcgttgg atctcgtttg gcagaaacgg agggcttgac ccaacgcttc
     1381 ggctgtggat cggtttttcg atcggttttc tgatcatctt ggcgataagt ttgagcgtat
     1441 tgagctttct actcaagcgc cgtttgaccc aaaaggtcgc tgacttgcgg aagagcgaag
     1501 aaaagtaccg acaaacggca gacaacagtc cggccgtcgt ttaccagctc cgtttggacc
     1561 ccgaaggcta cgcttctttt ctactcaaca tgtgcgtggt cattacaaaa accaaataag
     1621 cgaaaaactg aatgaaa
//
LOCUS       DAOPXN010000288         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_85362_length_1615_cov_4.926282, whole genome shotgun sequence.
ACCESSION   DAOPXN010000288 DAOPXN010000000
VERSION     DAOPXN010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85362_length_1615_cov_4.926282"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /gene="smpB"
                     /locus_tag="PKM49_10205"
     CDS             <1..430
                     /gene="smpB"
                     /locus_tag="PKM49_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HNY83208.1"
                     /translation="NKQAYREYFILEKKEAGLSLLGTEVKSLREGHLNLDDAFCKFKG
                     HELFLIKAKISAYSHASFFNHDPERDRKLLLNKRELRQWKTKVMEKGLTIVPLKLYFN
                     EQNLAKVEIALVKGKRLFDKREDLKRKAVQRDIERSQKWN"
     gene            444..>1615
                     /locus_tag="PKM49_10210"
     CDS             444..>1615
                     /locus_tag="PKM49_10210"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HNY83209.1"
                     /translation="MHIDAIIAEIGSTTTLLSAVDGLNTRNPVFLGQGEHYTTVQEGD
                     VTLGIERALKLLEQKVGEPLVWDTFLATSSAAGGLRVTVHGLVYDMTVKAAREAALGA
                     GAIIVHVTAGKLSEEDFETTRSMKPNMVLIAGGVDHGETDTVLHNARLFAKSPLDCPY
                     VYAGNTAASDRVRKILEEGGKRVTLTENVYPKVDELNVEPVRKVLQDLFSKHIIHARG
                     MEKIDEMVKYPIIPTPAAVMRTAQLLARRFEDVLCVDIGGATTDIDSVTQGNSELAEY
                     ASTPEPYAKRTVEGDLGVYVNASHVASLIGPEIEVTYPNFRDLLRQLTPYPQNPEMET
                     FAAYLAKYCFTTALRRHAGRLKYYYGPLGRKKTLEGRDLTYVKALFGTGGILSRSR"
BASE COUNT          426 a          428 c          432 g          329 t
ORIGIN      
        1 taacaagcag gcctaccgag agtattttat cttagagaaa aaagaagccg gcctctcctt
       61 attgggaacc gaagtgaagt cgttgcgaga aggccatctc aacctcgacg acgccttttg
      121 caaattcaaa ggtcacgagc tgtttctcat taaagccaaa atcagcgctt attcgcacgc
      181 aagttttttc aatcacgatc cggagcgtga cagaaaactg ttgttgaaca agcgcgagtt
      241 gcgccaatgg aagaccaaag tgatggaaaa gggtctcacg atcgtaccct taaagcttta
      301 cttcaacgag caaaacctcg caaaagtgga gatcgccttg gtgaaaggaa aacgtctgtt
      361 cgacaagcgc gaggatctga agcgtaaggc tgttcaacga gatatcgagc gatctcagaa
      421 atggaattga gggagagcgc cagatgcata tcgacgcgat cattgctgaa ataggctcaa
      481 cgaccacact cctttccgcc gttgacggtc taaacacgcg caatccggtc tttcttggac
      541 aaggagagca ttatactacg gttcaggagg gagatgtcac cttagggatc gaacgggctc
      601 tgaaactttt agaacaaaag gtcggagaac ccctcgtctg ggacacgttt ctcgcgacga
      661 gcagcgcggc cggagggttg cgagtgaccg ttcacgggct ggtctacgac atgaccgtca
      721 aagccgcgag ggaagccgcc ctaggcgccg gcgccatcat cgtccacgtc accgcgggga
      781 aactctctga ggaagatttc gaaactaccc gatcgatgaa gcccaatatg gtcctgatcg
      841 ccggcggagt cgatcatggc gaaaccgaca ccgttcttca caacgccaga ctgtttgcga
      901 aaagcccgct ggactgccct tacgtttacg ccggaaacac cgcggcttcc gatcgggtac
      961 gaaaaatact ggaggaaggc ggaaaacggg tgaccctgac ggaaaacgtc tatcccaagg
     1021 tggatgaatt gaacgtcgag ccggttcgaa aagtcctgca agatctcttt tctaagcata
     1081 tcatccacgc ccgcggaatg gaaaaaatag atgagatggt taagtatccg atcatcccga
     1141 ctccggccgc cgtgatgcgg accgcccagc tcttggcgcg ccgtttcgag gacgtcctct
     1201 gcgtcgatat cgggggcgct accaccgaca tagattccgt gacgcaaggt aactcggaac
     1261 tcgcggagta cgcctccaca cccgagcctt atgcgaaaag aaccgtcgaa ggggacctcg
     1321 gcgtctacgt caacgcttct cacgtcgcat ccttaatcgg cccggaaata gaagtcacct
     1381 accccaactt ccgcgatcta ttgcgccagt taactcctta cccgcaaaac ccggagatgg
     1441 aaactttcgc ggcttatcta gcgaaatatt gctttacaac ggctttgcgg agacacgccg
     1501 gtaggttaaa gtactactac gggcctctag gcagaaagaa gacgttagag ggacgggatc
     1561 tgacctacgt gaaggccttg tttggaacgg gcggcatact gagccgttct cggtt
//
LOCUS       DAOPXN010000289         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Thermotogota bacterium isolate AMR_MDS_1339
            NODE_87951_length_1585_cov_3.315686, whole genome shotgun sequence.
ACCESSION   DAOPXN010000289 DAOPXN010000000
VERSION     DAOPXN010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854544
            Sequence Read Archive: SRR4236662
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermotogota bacterium (wastewater metagenome)
  ORGANISM  Thermotogota bacterium
            Bacteria; Thermotogota.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 02:18:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,044
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,985
            CDSs (with protein)               :: 1,985
            Genes (RNA)                       :: 45
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 13 of 14
            Pseudo Genes (internal stop)      :: 0 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Thermotogota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87951_length_1585_cov_3.315686"
                     /isolate="AMR_MDS_1339"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053689"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-05-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="PKM49_10215"
     CDS             complement(<1..706)
                     /locus_tag="PKM49_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNY83210.1"
                     /translation="MITIVVFTIFVIAYALIMQGKYPRSVVAFAAGILILLLKVSPEL
                     TVGSVGEYVDFNTIGLLIGMMIIVGVLKSTGFFEFITAWIVKLSKGRMLRITLLMLVA
                     SALLSSILDNVTTILVFAPIVFLIADSIQINPTPLILALLFSANIGGTSTILGDPPNL
                     LIGSAANAGFIQFSSVMLWPTLIVLAIYTLYFRKKNAALAQIPSEKLERLLSIDPSKA
                     ITDRKLFKQSLIVFGFV"
     gene            complement(822..>1585)
                     /locus_tag="PKM49_10220"
     CDS             complement(822..>1585)
                     /locus_tag="PKM49_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006487009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNY83211.1"
                     /translation="IVTGKPLELGGSLGRTEATGRGVRVCTEEVVKYLTDKGKVNKPL
                     NQMRIAVQGFGNVGQHTARLISEELGAKIVAISDISGGYYKDSGFDVNDLMGYCAKNK
                     VIKGYPNADREISNQELLASDVDILIPAALENQITGANANNVRAKIIVEAANGPITPE
                     GDEILLKKGVFIVPDFLANAGGVTVSYFEWVQGMQWYFWDIDEVRKALHRLMRKAFAD
                     VAGAKEKYNTDMRSAAYVVSIEKVATVTKLRGIYP"
BASE COUNT          386 a          421 c          404 g          374 t
ORIGIN      
        1 tgacgaaccc aaaaacgatc aggctctgct tgaacagttt tctatcggtg atcgcctttg
       61 aggggtctat cgaaagcaag cgctcgagtt tttccgatgg gatttgggcg agcgctgcat
      121 tcttcttacg gaaatataag gtgtagatgg ccaaaacgat caaggtgggc cacagcatca
      181 ccgacgaaaa ctggatgaat ccggcattcg cagcactccc gatgagcagg ttgggcggat
      241 ccccgaggat cgtcgatgta ccgcctatat tcgcggaaaa caacaacgcg agaatgagcg
      301 gggtcgggtt aatctgtatc gaatcggcta tgaggaagac gatgggggcg aagacgagga
      361 tggtggtgac gttatcaaga atgcttgaca ggagcgcgga ggcaaccagc atcaagaggg
      421 taatccgtaa catacggcct ttagacagtt tgacgatcca ggcggtgatg aactcaaaga
      481 atccggtcga ctttagaacg cctacgatga tcatcattcc tatgagaagg ccgatggtgt
      541 tgaaatcgac gtactcgccc accgatccaa cggtcagttc cggagaaacc ttcaatagga
      601 gaatgagaat tccggcggcg aacgccacta ccgatcgggg gtacttcccc tgcataatca
      661 aagcgtaggc gatgacgaag atcgtaaaca ccacaatggt tatcaataga ctccctccct
      721 catatctcga cacgccgaag tatatcacaa tcgtcgggat cgtaaaaagc ccataaggta
      781 aaaaaatagg ggctttcgcc cctatttcaa taaatgggcc tttaagggta gatccctcgc
      841 aacttcgtca ccgtggccac tttttctata gatacgacgt aggcagccga acgcatatcg
      901 gtgttgtact tctctttcgc ccccgccaca tcggcgaagg cctttctcat gagccgatgc
      961 aaggcttttc gtacctcgtc gatatcccag aaataccatt gcataccctg tacccattcg
     1021 aagtaggaaa ctgtcacacc accggcgttg gctaagaagt ccggaacgat gaaaacgcct
     1081 ttcttcagaa ggatttcgtc tccttccgga gtgatcggac catttgccgc ttcgacgata
     1141 attttcgctc tcacgttatt ggcgtttgca cccgtgatct gattctccaa agcggccgga
     1201 atcagaatat cgacatcgga ggcgagcaat tcttggtttg aaatctcgcg atctgcgttc
     1261 gggtagcctt tgatcacttt gtttttcgcg cagtagccca tcagatcgtt cacatcgaat
     1321 ccggaatcct tatagtaccc gccggagatg tcgctgatcg cgacgatctt cgcgccaagt
     1381 tcttcggaga tgagccgggc cgtgtgctgt cccacgttcc caaacccttg aaccgctatc
     1441 ctcatctgat tcaacggttt gttgaccttg cccttgtccg tcaggtactt gacgacctct
     1501 tccgtgcata cgcgcacgcc ccgcccggtg gcttcggtcc tgccaaggga gccgccgagc
     1561 tccagaggtt ttcctgtgac gatgc
//
